CCDC158 cDNA ORF clone, Bos taurus(cattle)

The following CCDC158 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCDC158 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa52839 XM_010806251.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52839 XM_010797975.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52847 XM_010806249.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52847 XM_015463811.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa235595 XM_024993606.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235596 XM_024993607.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235597 XM_024993608.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa90109 XM_015463808.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90109 XM_015471691.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa52833 XM_010797969.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52833 XM_010806240.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52834 XM_010797970.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52834 XM_010806243.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52835 XM_010797971.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52835 XM_010806245.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52836 XM_010806246.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52836 XM_010797972.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52837 XM_010806248.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52837 XM_010797973.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52840 XM_015471690.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52840 XM_015463802.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52840 XM_015463801.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52840 XM_010806237.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52841 XM_010806238.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52841 XM_015463803.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52842 XM_010806239.2
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52842 XM_015463804.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52843 XM_010806241.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52843 XM_015463805.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52844 XM_010806242.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52844 XM_015463806.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52845 XM_010806244.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52845 XM_015463807.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52846 XM_015463810.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52846 XM_015471692.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52846 XM_010806247.3
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52846 XM_015463809.1
Latest version!
Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa52840 XM_010806237.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52840 XM_015471690.1 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52835 XM_010806245.1 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52846 XM_010806247.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52844 XM_010806242.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52834 XM_010806243.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52836 XM_010806246.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52833 XM_010806240.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52843 XM_010806241.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52841 XM_010806238.1 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52842 XM_010806239.1 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52845 XM_010806244.2 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa90109 XM_015471691.1 Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa52839
    Clone ID Related Accession (Same CDS sequence) XM_010806251.1 , XM_010797975.1
    Accession Version XM_010806251.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATAAGCAA CAACTCATTA AGGGAATCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804553.1
    CDS219..2912
    Translation

    Target ORF information:

    RefSeq Version XM_010806251.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806251.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATAAGCAA CAACTCATTA AGGGAATCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52839
    Clone ID Related Accession (Same CDS sequence) XM_010806251.1 , XM_010797975.1
    Accession Version XM_010797975.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATAAGCAA CAACTCATTA AGGGAATCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796277.1
    CDS219..2912
    Translation

    Target ORF information:

    RefSeq Version XM_010797975.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797975.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATAAGCAA CAACTCATTA AGGGAATCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52847
    Clone ID Related Accession (Same CDS sequence) XM_010806249.2 , XM_015463811.1
    Accession Version XM_010806249.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAGT AGAGGATCAG
    CTTGAAGCAC TGAAATCTGA ATCACAAAAC AAAATGGAAC TACTTCTTCA ACAACATCAA
    GATCGGATTG AACAGTTAAT AAGTGAACAT GAAGCTGAAA TAACAGGACT CACCGAGAAA
    GCTAGCAGTG CTCGGAGCCA AGCCAACAGT ATCCAGAGTC AATTGGAAAT CATTCAAGAG
    CAAGCAAGAA ACCAAAATTC AATGTATATG CGCCAACTCA GTGACCTGGA ATCTACTGTT
    TCTCAACTAC GTTCTGAATT AAGGGAAGCC AAAAGGATGT ATGAAGACAA GATAGAAGAG
    CTGGAGAAGC AGTTAGTTCT CGCAAACTCA GAGCTAACTG AAGCACGGAC GGAACGTGAT
    CAGTTCAGTC AGGAATCTGG AAATCTGGAC GACCAACTTC AAAAGCTCTT GGCCGACCTA
    CATAAACGAG AGAAGGAGCT GAGTCTGGAG AAGGAGCAGA ACAAGAGGCT GTGGGACCGG
    GACACGGGCA ACAGCATCAC CATCGACCAC CTGCGGCGGG AACTGGACGA CAGGAACATG
    GAGGTGCAGC GCCTGGAGGC CTTGCTCAAG GCCATGAAGA GCGAGTGTCA GGGCCAGATG
    GAGCGGCAGA TGGCAGCCAT TCAAGGAAAG AATGAAAGTC TAGAAAAAGT GTCCTCCTTG
    ACTGCTCAGC TTGAATCCAC CAAAGAGATG CTCCGCAAAG TGGTGGAAGA GTTGACAGCC
    AAGAAGATGA CCCTGGAGAG TTCGGAGAGG ACCGTGTCGG ACCTGACAGC TTCCCTCCAG
    GAAAAGGAGC GAGCCATCGA GGCCACCAAC GCAGAAATCA CGAAGCTGCG CTCCCGGGTG
    GACTTGAAGT TGCAGGAGCT GCAGCACCTG AAGAATGAAG GGGATCACCT CCGAAACGTG
    CAGACAGAGT GTGAGGCCCT CAGGCTGCAG ATGGCAGAGA AGGACAAAGT GATCGAGATC
    CTGCGACAGC AGATTGAGAA CATGACGCAG TTGGTGGGCC AGCACGGGCG GACGGCCGGT
    GCTATGCAAG TGGAGAAAGC TCAGCTGGAG AAGGAGATCA ATGATAGGAG GCTGGAGCTA
    CAGGAAGTTA AGATTTTGAA AGATAAAAGA GATGCAAAGA TACGGGAGCT TGAAGCCAGA
    GTGAGTGACC TGGAACTGGA AAAGGTGAAG CTGGTGAACG CAGGCTCCGA GAGGCTCCGT
    GCAGTGAAAG ACATCAAGCA AGAGAGAGAC CATTTATTAA GTGAGGTGAA AGCTAGTAGG
    AATGAGTTAA ACAGTCTTAC AGAGGACTAT GAAGTCTTAA AAAGGAATTT TCGAAACAAA
    AGTGAAGAAA TGGAAACTAC TACAAATAAG CTGAAAATGC AATTAAAATC TGCACAGTCT
    GAGCTGGAGC AAACAAGAAA TACATTAAAG TCAATGGAAG GATCTGATGG TCATGCTATG
    AAAGTGGCAA TGGGGATGCA AAAACAAATT ACAGCTAAAA GAGGTCAGAT AGATGCCCTT
    CAGAGCAAGA TACAATTTTT GGAAGAGGCA ATGACAAATG CAAACAAGGA GAAGCATTTT
    CTAAAAGAAG AGAAGACAAA ATTAAGCCAG GAATTGAGTA CTGTTTCAAC AGAGAAAAAC
    AAGATGGCCG GAGAACTAGA AGTCCTGCGA TCTCAGGAGC GCCGATTGAA AGAAAAGGTT
    GCTAATATGG AAGTTGCTCT TGATAAGGCA TCTTTGCAGT TTGCAGAATG TCAAGATATA
    ATACAGCGTC AGGAACAAGA ATCTGTGCGC CTAAAGCTTC AACACACATT GGATGTAAAA
    GAACTGCAGG GCCCTGGATA CACCTCAAAT TCTGCTGTGA AACCACGCCT TCTACAACCA
    TCACCTGCTG CTCGTTCTCA TTCCAATGTT CCATCTTCCC AGTCTACAGC CAGCTTCCTG
    TCTCATCACT CTATGAAACC TAACGCAAAG GAAGATCCAA CGAGGGACTT GAAACAGCTT
    CTCCAGGAGT TGAGAAGTGT GATCAACGAG GAGCCAGCCG TGCCTCTGGG CAAGAGCGAA
    GAAGACGGGA GAGCACCATC TCTGGGGGTC TCCTTTGTGG CTGTAAGCAA CAACTCATTA
    AGGGAATCCA CTGAAGGCAG CAAATCAAGT GAAACTTTTA GCAGAGAGCC AGTCACGTTA
    CACGTAGCAG AGTTGGAAGA TCCGCCTGGT TGCTTTGTAT TCCCATCTGC AGCTTACACA
    GAATCGTTGT CACGTATTTT AATTCTAAAG GTTTCTCAGG AGACAGGAAG TCCATCTGTG
    AAAAATTCAG CTTCCCGTTC ATTCCATTCT TCTCCTAAGA AGTCTCCAGT TCACTCACTC
    CTGACTAGTT CAGTTGAGGA TTCCATAGGT TCCTCACCAC AATATAGATC TACCAAACCT
    GTTCATTCAC CTGATTCTGT TAAAGTCTCA GCCAATAGAA ACAACCGGAA AGACATGCAG
    GAAGCTCCAA ACCAGACTGG AAAGCTTGCA AACTCTGGTA GAAGATTTAC AGATGAAGAA
    CCAAGCAATG TCTTCAATGA TCAGAAATCA AGAAAAGAGA ATACAGAAAG TGAAAGACCA
    GGAAAAAATG TTACTAAAAT GATACATTGC TTGCCTATTC TTTACAGAGA GATGATGCCC
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804551.1
    CDS219..3164
    Translation

    Target ORF information:

    RefSeq Version XM_010806249.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806249.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAGT AGAGGATCAG
    CTTGAAGCAC TGAAATCTGA ATCACAAAAC AAAATGGAAC TACTTCTTCA ACAACATCAA
    GATCGGATTG AACAGTTAAT AAGTGAACAT GAAGCTGAAA TAACAGGACT CACCGAGAAA
    GCTAGCAGTG CTCGGAGCCA AGCCAACAGT ATCCAGAGTC AATTGGAAAT CATTCAAGAG
    CAAGCAAGAA ACCAAAATTC AATGTATATG CGCCAACTCA GTGACCTGGA ATCTACTGTT
    TCTCAACTAC GTTCTGAATT AAGGGAAGCC AAAAGGATGT ATGAAGACAA GATAGAAGAG
    CTGGAGAAGC AGTTAGTTCT CGCAAACTCA GAGCTAACTG AAGCACGGAC GGAACGTGAT
    CAGTTCAGTC AGGAATCTGG AAATCTGGAC GACCAACTTC AAAAGCTCTT GGCCGACCTA
    CATAAACGAG AGAAGGAGCT GAGTCTGGAG AAGGAGCAGA ACAAGAGGCT GTGGGACCGG
    GACACGGGCA ACAGCATCAC CATCGACCAC CTGCGGCGGG AACTGGACGA CAGGAACATG
    GAGGTGCAGC GCCTGGAGGC CTTGCTCAAG GCCATGAAGA GCGAGTGTCA GGGCCAGATG
    GAGCGGCAGA TGGCAGCCAT TCAAGGAAAG AATGAAAGTC TAGAAAAAGT GTCCTCCTTG
    ACTGCTCAGC TTGAATCCAC CAAAGAGATG CTCCGCAAAG TGGTGGAAGA GTTGACAGCC
    AAGAAGATGA CCCTGGAGAG TTCGGAGAGG ACCGTGTCGG ACCTGACAGC TTCCCTCCAG
    GAAAAGGAGC GAGCCATCGA GGCCACCAAC GCAGAAATCA CGAAGCTGCG CTCCCGGGTG
    GACTTGAAGT TGCAGGAGCT GCAGCACCTG AAGAATGAAG GGGATCACCT CCGAAACGTG
    CAGACAGAGT GTGAGGCCCT CAGGCTGCAG ATGGCAGAGA AGGACAAAGT GATCGAGATC
    CTGCGACAGC AGATTGAGAA CATGACGCAG TTGGTGGGCC AGCACGGGCG GACGGCCGGT
    GCTATGCAAG TGGAGAAAGC TCAGCTGGAG AAGGAGATCA ATGATAGGAG GCTGGAGCTA
    CAGGAAGTTA AGATTTTGAA AGATAAAAGA GATGCAAAGA TACGGGAGCT TGAAGCCAGA
    GTGAGTGACC TGGAACTGGA AAAGGTGAAG CTGGTGAACG CAGGCTCCGA GAGGCTCCGT
    GCAGTGAAAG ACATCAAGCA AGAGAGAGAC CATTTATTAA GTGAGGTGAA AGCTAGTAGG
    AATGAGTTAA ACAGTCTTAC AGAGGACTAT GAAGTCTTAA AAAGGAATTT TCGAAACAAA
    AGTGAAGAAA TGGAAACTAC TACAAATAAG CTGAAAATGC AATTAAAATC TGCACAGTCT
    GAGCTGGAGC AAACAAGAAA TACATTAAAG TCAATGGAAG GATCTGATGG TCATGCTATG
    AAAGTGGCAA TGGGGATGCA AAAACAAATT ACAGCTAAAA GAGGTCAGAT AGATGCCCTT
    CAGAGCAAGA TACAATTTTT GGAAGAGGCA ATGACAAATG CAAACAAGGA GAAGCATTTT
    CTAAAAGAAG AGAAGACAAA ATTAAGCCAG GAATTGAGTA CTGTTTCAAC AGAGAAAAAC
    AAGATGGCCG GAGAACTAGA AGTCCTGCGA TCTCAGGAGC GCCGATTGAA AGAAAAGGTT
    GCTAATATGG AAGTTGCTCT TGATAAGGCA TCTTTGCAGT TTGCAGAATG TCAAGATATA
    ATACAGCGTC AGGAACAAGA ATCTGTGCGC CTAAAGCTTC AACACACATT GGATGTAAAA
    GAACTGCAGG GCCCTGGATA CACCTCAAAT TCTGCTGTGA AACCACGCCT TCTACAACCA
    TCACCTGCTG CTCGTTCTCA TTCCAATGTT CCATCTTCCC AGTCTACAGC CAGCTTCCTG
    TCTCATCACT CTATGAAACC TAACGCAAAG GAAGATCCAA CGAGGGACTT GAAACAGCTT
    CTCCAGGAGT TGAGAAGTGT GATCAACGAG GAGCCAGCCG TGCCTCTGGG CAAGAGCGAA
    GAAGACGGGA GAGCACCATC TCTGGGGGTC TCCTTTGTGG CTGTAAGCAA CAACTCATTA
    AGGGAATCCA CTGAAGGCAG CAAATCAAGT GAAACTTTTA GCAGAGAGCC AGTCACGTTA
    CACGTAGCAG AGTTGGAAGA TCCGCCTGGT TGCTTTGTAT TCCCATCTGC AGCTTACACA
    GAATCGTTGT CACGTATTTT AATTCTAAAG GTTTCTCAGG AGACAGGAAG TCCATCTGTG
    AAAAATTCAG CTTCCCGTTC ATTCCATTCT TCTCCTAAGA AGTCTCCAGT TCACTCACTC
    CTGACTAGTT CAGTTGAGGA TTCCATAGGT TCCTCACCAC AATATAGATC TACCAAACCT
    GTTCATTCAC CTGATTCTGT TAAAGTCTCA GCCAATAGAA ACAACCGGAA AGACATGCAG
    GAAGCTCCAA ACCAGACTGG AAAGCTTGCA AACTCTGGTA GAAGATTTAC AGATGAAGAA
    CCAAGCAATG TCTTCAATGA TCAGAAATCA AGAAAAGAGA ATACAGAAAG TGAAAGACCA
    GGAAAAAATG TTACTAAAAT GATACATTGC TTGCCTATTC TTTACAGAGA GATGATGCCC
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52847
    Clone ID Related Accession (Same CDS sequence) XM_010806249.2 , XM_015463811.1
    Accession Version XM_015463811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAGT AGAGGATCAG
    CTTGAAGCAC TGAAATCTGA ATCACAAAAC AAAATGGAAC TACTTCTTCA ACAACATCAA
    GATCGGATTG AACAGTTAAT AAGTGAACAT GAAGCTGAAA TAACAGGACT CACCGAGAAA
    GCTAGCAGTG CTCGGAGCCA AGCCAACAGT ATCCAGAGTC AATTGGAAAT CATTCAAGAG
    CAAGCAAGAA ACCAAAATTC AATGTATATG CGCCAACTCA GTGACCTGGA ATCTACTGTT
    TCTCAACTAC GTTCTGAATT AAGGGAAGCC AAAAGGATGT ATGAAGACAA GATAGAAGAG
    CTGGAGAAGC AGTTAGTTCT CGCAAACTCA GAGCTAACTG AAGCACGGAC GGAACGTGAT
    CAGTTCAGTC AGGAATCTGG AAATCTGGAC GACCAACTTC AAAAGCTCTT GGCCGACCTA
    CATAAACGAG AGAAGGAGCT GAGTCTGGAG AAGGAGCAGA ACAAGAGGCT GTGGGACCGG
    GACACGGGCA ACAGCATCAC CATCGACCAC CTGCGGCGGG AACTGGACGA CAGGAACATG
    GAGGTGCAGC GCCTGGAGGC CTTGCTCAAG GCCATGAAGA GCGAGTGTCA GGGCCAGATG
    GAGCGGCAGA TGGCAGCCAT TCAAGGAAAG AATGAAAGTC TAGAAAAAGT GTCCTCCTTG
    ACTGCTCAGC TTGAATCCAC CAAAGAGATG CTCCGCAAAG TGGTGGAAGA GTTGACAGCC
    AAGAAGATGA CCCTGGAGAG TTCGGAGAGG ACCGTGTCGG ACCTGACAGC TTCCCTCCAG
    GAAAAGGAGC GAGCCATCGA GGCCACCAAC GCAGAAATCA CGAAGCTGCG CTCCCGGGTG
    GACTTGAAGT TGCAGGAGCT GCAGCACCTG AAGAATGAAG GGGATCACCT CCGAAACGTG
    CAGACAGAGT GTGAGGCCCT CAGGCTGCAG ATGGCAGAGA AGGACAAAGT GATCGAGATC
    CTGCGACAGC AGATTGAGAA CATGACGCAG TTGGTGGGCC AGCACGGGCG GACGGCCGGT
    GCTATGCAAG TGGAGAAAGC TCAGCTGGAG AAGGAGATCA ATGATAGGAG GCTGGAGCTA
    CAGGAAGTTA AGATTTTGAA AGATAAAAGA GATGCAAAGA TACGGGAGCT TGAAGCCAGA
    GTGAGTGACC TGGAACTGGA AAAGGTGAAG CTGGTGAACG CAGGCTCCGA GAGGCTCCGT
    GCAGTGAAAG ACATCAAGCA AGAGAGAGAC CATTTATTAA GTGAGGTGAA AGCTAGTAGG
    AATGAGTTAA ACAGTCTTAC AGAGGACTAT GAAGTCTTAA AAAGGAATTT TCGAAACAAA
    AGTGAAGAAA TGGAAACTAC TACAAATAAG CTGAAAATGC AATTAAAATC TGCACAGTCT
    GAGCTGGAGC AAACAAGAAA TACATTAAAG TCAATGGAAG GATCTGATGG TCATGCTATG
    AAAGTGGCAA TGGGGATGCA AAAACAAATT ACAGCTAAAA GAGGTCAGAT AGATGCCCTT
    CAGAGCAAGA TACAATTTTT GGAAGAGGCA ATGACAAATG CAAACAAGGA GAAGCATTTT
    CTAAAAGAAG AGAAGACAAA ATTAAGCCAG GAATTGAGTA CTGTTTCAAC AGAGAAAAAC
    AAGATGGCCG GAGAACTAGA AGTCCTGCGA TCTCAGGAGC GCCGATTGAA AGAAAAGGTT
    GCTAATATGG AAGTTGCTCT TGATAAGGCA TCTTTGCAGT TTGCAGAATG TCAAGATATA
    ATACAGCGTC AGGAACAAGA ATCTGTGCGC CTAAAGCTTC AACACACATT GGATGTAAAA
    GAACTGCAGG GCCCTGGATA CACCTCAAAT TCTGCTGTGA AACCACGCCT TCTACAACCA
    TCACCTGCTG CTCGTTCTCA TTCCAATGTT CCATCTTCCC AGTCTACAGC CAGCTTCCTG
    TCTCATCACT CTATGAAACC TAACGCAAAG GAAGATCCAA CGAGGGACTT GAAACAGCTT
    CTCCAGGAGT TGAGAAGTGT GATCAACGAG GAGCCAGCCG TGCCTCTGGG CAAGAGCGAA
    GAAGACGGGA GAGCACCATC TCTGGGGGTC TCCTTTGTGG CTGTAAGCAA CAACTCATTA
    AGGGAATCCA CTGAAGGCAG CAAATCAAGT GAAACTTTTA GCAGAGAGCC AGTCACGTTA
    CACGTAGCAG AGTTGGAAGA TCCGCCTGGT TGCTTTGTAT TCCCATCTGC AGCTTACACA
    GAATCGTTGT CACGTATTTT AATTCTAAAG GTTTCTCAGG AGACAGGAAG TCCATCTGTG
    AAAAATTCAG CTTCCCGTTC ATTCCATTCT TCTCCTAAGA AGTCTCCAGT TCACTCACTC
    CTGACTAGTT CAGTTGAGGA TTCCATAGGT TCCTCACCAC AATATAGATC TACCAAACCT
    GTTCATTCAC CTGATTCTGT TAAAGTCTCA GCCAATAGAA ACAACCGGAA AGACATGCAG
    GAAGCTCCAA ACCAGACTGG AAAGCTTGCA AACTCTGGTA GAAGATTTAC AGATGAAGAA
    CCAAGCAATG TCTTCAATGA TCAGAAATCA AGAAAAGAGA ATACAGAAAG TGAAAGACCA
    GGAAAAAATG TTACTAAAAT GATACATTGC TTGCCTATTC TTTACAGAGA GATGATGCCC
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319297.1
    CDS219..3164
    Translation

    Target ORF information:

    RefSeq Version XM_015463811.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAGT AGAGGATCAG
    CTTGAAGCAC TGAAATCTGA ATCACAAAAC AAAATGGAAC TACTTCTTCA ACAACATCAA
    GATCGGATTG AACAGTTAAT AAGTGAACAT GAAGCTGAAA TAACAGGACT CACCGAGAAA
    GCTAGCAGTG CTCGGAGCCA AGCCAACAGT ATCCAGAGTC AATTGGAAAT CATTCAAGAG
    CAAGCAAGAA ACCAAAATTC AATGTATATG CGCCAACTCA GTGACCTGGA ATCTACTGTT
    TCTCAACTAC GTTCTGAATT AAGGGAAGCC AAAAGGATGT ATGAAGACAA GATAGAAGAG
    CTGGAGAAGC AGTTAGTTCT CGCAAACTCA GAGCTAACTG AAGCACGGAC GGAACGTGAT
    CAGTTCAGTC AGGAATCTGG AAATCTGGAC GACCAACTTC AAAAGCTCTT GGCCGACCTA
    CATAAACGAG AGAAGGAGCT GAGTCTGGAG AAGGAGCAGA ACAAGAGGCT GTGGGACCGG
    GACACGGGCA ACAGCATCAC CATCGACCAC CTGCGGCGGG AACTGGACGA CAGGAACATG
    GAGGTGCAGC GCCTGGAGGC CTTGCTCAAG GCCATGAAGA GCGAGTGTCA GGGCCAGATG
    GAGCGGCAGA TGGCAGCCAT TCAAGGAAAG AATGAAAGTC TAGAAAAAGT GTCCTCCTTG
    ACTGCTCAGC TTGAATCCAC CAAAGAGATG CTCCGCAAAG TGGTGGAAGA GTTGACAGCC
    AAGAAGATGA CCCTGGAGAG TTCGGAGAGG ACCGTGTCGG ACCTGACAGC TTCCCTCCAG
    GAAAAGGAGC GAGCCATCGA GGCCACCAAC GCAGAAATCA CGAAGCTGCG CTCCCGGGTG
    GACTTGAAGT TGCAGGAGCT GCAGCACCTG AAGAATGAAG GGGATCACCT CCGAAACGTG
    CAGACAGAGT GTGAGGCCCT CAGGCTGCAG ATGGCAGAGA AGGACAAAGT GATCGAGATC
    CTGCGACAGC AGATTGAGAA CATGACGCAG TTGGTGGGCC AGCACGGGCG GACGGCCGGT
    GCTATGCAAG TGGAGAAAGC TCAGCTGGAG AAGGAGATCA ATGATAGGAG GCTGGAGCTA
    CAGGAAGTTA AGATTTTGAA AGATAAAAGA GATGCAAAGA TACGGGAGCT TGAAGCCAGA
    GTGAGTGACC TGGAACTGGA AAAGGTGAAG CTGGTGAACG CAGGCTCCGA GAGGCTCCGT
    GCAGTGAAAG ACATCAAGCA AGAGAGAGAC CATTTATTAA GTGAGGTGAA AGCTAGTAGG
    AATGAGTTAA ACAGTCTTAC AGAGGACTAT GAAGTCTTAA AAAGGAATTT TCGAAACAAA
    AGTGAAGAAA TGGAAACTAC TACAAATAAG CTGAAAATGC AATTAAAATC TGCACAGTCT
    GAGCTGGAGC AAACAAGAAA TACATTAAAG TCAATGGAAG GATCTGATGG TCATGCTATG
    AAAGTGGCAA TGGGGATGCA AAAACAAATT ACAGCTAAAA GAGGTCAGAT AGATGCCCTT
    CAGAGCAAGA TACAATTTTT GGAAGAGGCA ATGACAAATG CAAACAAGGA GAAGCATTTT
    CTAAAAGAAG AGAAGACAAA ATTAAGCCAG GAATTGAGTA CTGTTTCAAC AGAGAAAAAC
    AAGATGGCCG GAGAACTAGA AGTCCTGCGA TCTCAGGAGC GCCGATTGAA AGAAAAGGTT
    GCTAATATGG AAGTTGCTCT TGATAAGGCA TCTTTGCAGT TTGCAGAATG TCAAGATATA
    ATACAGCGTC AGGAACAAGA ATCTGTGCGC CTAAAGCTTC AACACACATT GGATGTAAAA
    GAACTGCAGG GCCCTGGATA CACCTCAAAT TCTGCTGTGA AACCACGCCT TCTACAACCA
    TCACCTGCTG CTCGTTCTCA TTCCAATGTT CCATCTTCCC AGTCTACAGC CAGCTTCCTG
    TCTCATCACT CTATGAAACC TAACGCAAAG GAAGATCCAA CGAGGGACTT GAAACAGCTT
    CTCCAGGAGT TGAGAAGTGT GATCAACGAG GAGCCAGCCG TGCCTCTGGG CAAGAGCGAA
    GAAGACGGGA GAGCACCATC TCTGGGGGTC TCCTTTGTGG CTGTAAGCAA CAACTCATTA
    AGGGAATCCA CTGAAGGCAG CAAATCAAGT GAAACTTTTA GCAGAGAGCC AGTCACGTTA
    CACGTAGCAG AGTTGGAAGA TCCGCCTGGT TGCTTTGTAT TCCCATCTGC AGCTTACACA
    GAATCGTTGT CACGTATTTT AATTCTAAAG GTTTCTCAGG AGACAGGAAG TCCATCTGTG
    AAAAATTCAG CTTCCCGTTC ATTCCATTCT TCTCCTAAGA AGTCTCCAGT TCACTCACTC
    CTGACTAGTT CAGTTGAGGA TTCCATAGGT TCCTCACCAC AATATAGATC TACCAAACCT
    GTTCATTCAC CTGATTCTGT TAAAGTCTCA GCCAATAGAA ACAACCGGAA AGACATGCAG
    GAAGCTCCAA ACCAGACTGG AAAGCTTGCA AACTCTGGTA GAAGATTTAC AGATGAAGAA
    CCAAGCAATG TCTTCAATGA TCAGAAATCA AGAAAAGAGA ATACAGAAAG TGAAAGACCA
    GGAAAAAATG TTACTAAAAT GATACATTGC TTGCCTATTC TTTACAGAGA GATGATGCCC
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235595
    Clone ID Related Accession (Same CDS sequence) XM_024993606.1
    Accession Version XM_024993606.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGTTTGC AGAATGTCAA GATATAATAC AGCGTCAGGA ACAAGAATCT
    GTGCGCCTAA AGCTTCAACA CACATTGGAT GTAAAAGAAC TGCAGGGCCC TGGATACACC
    TCAAATTCTG CTGTGAAACC ACGCCTTCTA CAACCATCAC CTGCTGCTCG TTCTCATTCC
    AATGTTCCAT CTTCCCAGTC TACAGCCAGC TTCCTGTCTC ATCACTCTAT GAAACCTAAC
    GCAAAGGAAG ATCCAACGAG GGACTTGAAA CAGCTTCTCC AGGAGTTGAG AAGTGTGATC
    AACGAGGAGC CAGCCGTGCC TCTGGGCAAG AGCGAAGAAG ACGGGAGAGC ACCATCTCTG
    GGGGTCTCCT TTGTGGCTGT AAGCAACAAC TCATTAAGGG AATCCACTGA AGGCAGCAAA
    TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC ACGTTACACG TAGCAGAGTT GGAAGATCCG
    CCTGGTTGCT TTGTATTCCC ATCTGCAGCT TACACAGAAT CGTTGTCACG TATTTTAATT
    CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA TCTGTGAAAA ATTCAGCTTC CCGTTCATTC
    CATTCTTCTC CTAAGAAGTC TCCAGTTCAC TCACTCCTGA CTAGTTCAGT TGAGGATTCC
    ATAGGTTCCT CACCACAATA TAGATCTACC AAACCTGTTC ATTCACCTGA TTCTGTTAAA
    GTCTCAGCCA ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG
    CTTGCAAACT CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG
    AAATCAAGAA AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGATA
    CATTGCTTGC CTATTCTTTA CAGAGAGATG ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849374.1
    CDS240..3581
    Translation

    Target ORF information:

    RefSeq Version XM_024993606.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGTTTGC AGAATGTCAA GATATAATAC AGCGTCAGGA ACAAGAATCT
    GTGCGCCTAA AGCTTCAACA CACATTGGAT GTAAAAGAAC TGCAGGGCCC TGGATACACC
    TCAAATTCTG CTGTGAAACC ACGCCTTCTA CAACCATCAC CTGCTGCTCG TTCTCATTCC
    AATGTTCCAT CTTCCCAGTC TACAGCCAGC TTCCTGTCTC ATCACTCTAT GAAACCTAAC
    GCAAAGGAAG ATCCAACGAG GGACTTGAAA CAGCTTCTCC AGGAGTTGAG AAGTGTGATC
    AACGAGGAGC CAGCCGTGCC TCTGGGCAAG AGCGAAGAAG ACGGGAGAGC ACCATCTCTG
    GGGGTCTCCT TTGTGGCTGT AAGCAACAAC TCATTAAGGG AATCCACTGA AGGCAGCAAA
    TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC ACGTTACACG TAGCAGAGTT GGAAGATCCG
    CCTGGTTGCT TTGTATTCCC ATCTGCAGCT TACACAGAAT CGTTGTCACG TATTTTAATT
    CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA TCTGTGAAAA ATTCAGCTTC CCGTTCATTC
    CATTCTTCTC CTAAGAAGTC TCCAGTTCAC TCACTCCTGA CTAGTTCAGT TGAGGATTCC
    ATAGGTTCCT CACCACAATA TAGATCTACC AAACCTGTTC ATTCACCTGA TTCTGTTAAA
    GTCTCAGCCA ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG
    CTTGCAAACT CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG
    AAATCAAGAA AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGATA
    CATTGCTTGC CTATTCTTTA CAGAGAGATG ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235596
    Clone ID Related Accession (Same CDS sequence) XM_024993607.1
    Accession Version XM_024993607.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849375.1
    CDS239..3526
    Translation

    Target ORF information:

    RefSeq Version XM_024993607.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993607.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235597
    Clone ID Related Accession (Same CDS sequence) XM_024993608.1
    Accession Version XM_024993608.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGCTT CACAGTCTCA GCCAATAGAA
    ACAACCGGAA AGACATGCAG GAAGCTCCAA ACCAGACTGG AAAGCTTGCA AACTCTGGTA
    GAAGATTTAC AGATGAAGAA CCAAGCAATG TCTTCAATGA TCAGAAATCA AGAAAAGAGA
    ATACAGAAAG TGAAAGACCA GGAAAAAATG TTACTAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849376.1
    CDS239..3520
    Translation

    Target ORF information:

    RefSeq Version XM_024993608.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993608.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGCTT CACAGTCTCA GCCAATAGAA
    ACAACCGGAA AGACATGCAG GAAGCTCCAA ACCAGACTGG AAAGCTTGCA AACTCTGGTA
    GAAGATTTAC AGATGAAGAA CCAAGCAATG TCTTCAATGA TCAGAAATCA AGAAAAGAGA
    ATACAGAAAG TGAAAGACCA GGAAAAAATG TTACTAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90109
    Clone ID Related Accession (Same CDS sequence) XM_015471691.1 , XM_015463808.1 , XM_015471691.2
    Accession Version XM_015471691.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGTCT CAGCCAATAG AAACAACCGG
    AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG CAAACTCTGG TAGAAGATTT
    ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT CAAGAAAAGA GAATACAGAA
    AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGATACATT GCTTGCCTAT TCTTTACAGA
    GAGATGATGC CCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327177.1
    CDS219..3476
    Translation

    Target ORF information:

    RefSeq Version XM_015471691.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGTCT CAGCCAATAG AAACAACCGG
    AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG CAAACTCTGG TAGAAGATTT
    ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT CAAGAAAAGA GAATACAGAA
    AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGATACATT GCTTGCCTAT TCTTTACAGA
    GAGATGATGC CCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90109
    Clone ID Related Accession (Same CDS sequence) XM_015471691.1 , XM_015463808.1 , XM_015471691.2
    Accession Version XM_015463808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGTCT CAGCCAATAG AAACAACCGG
    AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG CAAACTCTGG TAGAAGATTT
    ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT CAAGAAAAGA GAATACAGAA
    AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGATACATT GCTTGCCTAT TCTTTACAGA
    GAGATGATGC CCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319294.1
    CDS219..3476
    Translation

    Target ORF information:

    RefSeq Version XM_015463808.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGTCT CAGCCAATAG AAACAACCGG
    AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG CAAACTCTGG TAGAAGATTT
    ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT CAAGAAAAGA GAATACAGAA
    AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGATACATT GCTTGCCTAT TCTTTACAGA
    GAGATGATGC CCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90109
    Clone ID Related Accession (Same CDS sequence) XM_015471691.1 , XM_015463808.1 , XM_015471691.2
    Accession Version XM_015471691.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGTCT CAGCCAATAG AAACAACCGG
    AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG CAAACTCTGG TAGAAGATTT
    ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT CAAGAAAAGA GAATACAGAA
    AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGATACATT GCTTGCCTAT TCTTTACAGA
    GAGATGATGC CCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327177.1
    CDS239..3496
    Translation

    Target ORF information:

    RefSeq Version XM_015471691.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471691.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGGA
    AGTCCATCTG TGAAAAATTC AGCTTCCCGT TCATTCCATT CTTCTCCTAA GAAGTCTCCA
    GTTCACTCAC TCCTGACTAG TTCAGTTGAG GATTCCATAG GTTCCTCACC ACAATATAGA
    TCTACCAAAC CTGTTCATTC ACCTGATTCT GTTAAAGTCT CAGCCAATAG AAACAACCGG
    AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG CAAACTCTGG TAGAAGATTT
    ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT CAAGAAAAGA GAATACAGAA
    AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGATACATT GCTTGCCTAT TCTTTACAGA
    GAGATGATGC CCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52833
    Clone ID Related Accession (Same CDS sequence) XM_010806240.3 , XM_010797969.2 , XM_010806240.2
    Accession Version XM_010806240.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806240.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804542.1
    CDS239..3586
    Translation

    Target ORF information:

    RefSeq Version XM_010806240.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806240.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52833
    Clone ID Related Accession (Same CDS sequence) XM_010806240.3 , XM_010797969.2 , XM_010806240.2
    Accession Version XM_010797969.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010797969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796271.1
    CDS219..3566
    Translation

    Target ORF information:

    RefSeq Version XM_010797969.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797969.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52833
    Clone ID Related Accession (Same CDS sequence) XM_010806240.3 , XM_010797969.2 , XM_010806240.2
    Accession Version XM_010806240.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804542.1
    CDS219..3566
    Translation

    Target ORF information:

    RefSeq Version XM_010806240.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806240.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCTTCACA GTCTCAGCCA
    ATAGAAACAA CCGGAAAGAC ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT
    CTGGTAGAAG ATTTACAGAT GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA
    AAGAGAATAC AGAAAGTGAA AGACCAGGAA AAAATGTTAC TAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52834
    Clone ID Related Accession (Same CDS sequence) XM_010806243.2 , XM_010797970.2 , XM_010806243.3
    Accession Version XM_010806243.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804545.1
    CDS218..3511
    Translation

    Target ORF information:

    RefSeq Version XM_010806243.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52834
    Clone ID Related Accession (Same CDS sequence) XM_010806243.2 , XM_010797970.2 , XM_010806243.3
    Accession Version XM_010797970.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010797970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796272.1
    CDS218..3511
    Translation

    Target ORF information:

    RefSeq Version XM_010797970.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797970.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52834
    Clone ID Related Accession (Same CDS sequence) XM_010806243.2 , XM_010797970.2 , XM_010806243.3
    Accession Version XM_010806243.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806243.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804545.1
    CDS239..3532
    Translation

    Target ORF information:

    RefSeq Version XM_010806243.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806243.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52835
    Clone ID Related Accession (Same CDS sequence) XM_010806245.2 , XM_010806245.1 , XM_010797971.2
    Accession Version XM_010806245.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCAATGTC TTCAATGATC
    AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804547.1
    CDS240..3479
    Translation

    Target ORF information:

    RefSeq Version XM_010806245.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCAATGTC TTCAATGATC
    AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52835
    Clone ID Related Accession (Same CDS sequence) XM_010806245.2 , XM_010806245.1 , XM_010797971.2
    Accession Version XM_010806245.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCAATGTC TTCAATGATC
    AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804547.1
    CDS219..3458
    Translation

    Target ORF information:

    RefSeq Version XM_010806245.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCAATGTC TTCAATGATC
    AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52835
    Clone ID Related Accession (Same CDS sequence) XM_010806245.2 , XM_010806245.1 , XM_010797971.2
    Accession Version XM_010797971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010797971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCAATGTC TTCAATGATC
    AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796273.1
    CDS219..3458
    Translation

    Target ORF information:

    RefSeq Version XM_010797971.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGCAATGTC TTCAATGATC
    AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52836
    Clone ID Related Accession (Same CDS sequence) XM_010806246.2 , XM_010797972.2 , XM_010806246.3
    Accession Version XM_010806246.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804548.1
    CDS218..3451
    Translation

    Target ORF information:

    RefSeq Version XM_010806246.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52836
    Clone ID Related Accession (Same CDS sequence) XM_010806246.2 , XM_010797972.2 , XM_010806246.3
    Accession Version XM_010797972.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010797972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796274.1
    CDS218..3451
    Translation

    Target ORF information:

    RefSeq Version XM_010797972.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797972.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52836
    Clone ID Related Accession (Same CDS sequence) XM_010806246.2 , XM_010797972.2 , XM_010806246.3
    Accession Version XM_010806246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804548.1
    CDS239..3472
    Translation

    Target ORF information:

    RefSeq Version XM_010806246.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGC TTCACAGTCT
    CAGCCAATAG AAACAACCGG AAAGACATGC AGGAAGCTCC AAACCAGACT GGAAAGCTTG
    CAAACTCTGG TAGAAGATTT ACAGATGAAG AACCAAGCAA TGTCTTCAAT GATCAGAAAT
    CAAGAAAAGA GAATACAGAA AGTGAAAGAC CAGGAAAAAA TGTTACTAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52837
    Clone ID Related Accession (Same CDS sequence) XM_010797973.2 , XM_010806248.2
    Accession Version XM_010797973.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010797973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGA AGTCCATCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796275.1
    CDS219..3221
    Translation

    Target ORF information:

    RefSeq Version XM_010797973.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797973.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGA AGTCCATCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52837
    Clone ID Related Accession (Same CDS sequence) XM_010797973.2 , XM_010806248.2
    Accession Version XM_010806248.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGA AGTCCATCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804550.1
    CDS219..3221
    Translation

    Target ORF information:

    RefSeq Version XM_010806248.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGA AGTCCATCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52840
    Clone ID Related Accession (Same CDS sequence) XM_010806237.3 , XM_015463802.1 , XM_010806237.2 , XM_015471690.1 , XM_015471690.2 , XM_015463801.1
    Accession Version XM_010806237.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804539.1
    CDS239..3622
    Translation

    Target ORF information:

    RefSeq Version XM_010806237.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806237.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52840
    Clone ID Related Accession (Same CDS sequence) XM_010806237.3 , XM_015463802.1 , XM_010806237.2 , XM_015471690.1 , XM_015471690.2 , XM_015463801.1
    Accession Version XM_015463802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319288.1
    CDS167..3550
    Translation

    Target ORF information:

    RefSeq Version XM_015463802.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52840
    Clone ID Related Accession (Same CDS sequence) XM_010806237.3 , XM_015463802.1 , XM_010806237.2 , XM_015471690.1 , XM_015471690.2 , XM_015463801.1
    Accession Version XM_010806237.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804539.1
    CDS219..3602
    Translation

    Target ORF information:

    RefSeq Version XM_010806237.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52840
    Clone ID Related Accession (Same CDS sequence) XM_010806237.3 , XM_015463802.1 , XM_010806237.2 , XM_015471690.1 , XM_015471690.2 , XM_015463801.1
    Accession Version XM_015471690.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327176.1
    CDS167..3550
    Translation

    Target ORF information:

    RefSeq Version XM_015471690.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52840
    Clone ID Related Accession (Same CDS sequence) XM_010806237.3 , XM_015463802.1 , XM_010806237.2 , XM_015471690.1 , XM_015471690.2 , XM_015463801.1
    Accession Version XM_015471690.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327176.1
    CDS177..3560
    Translation

    Target ORF information:

    RefSeq Version XM_015471690.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471690.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52840
    Clone ID Related Accession (Same CDS sequence) XM_010806237.3 , XM_015463802.1 , XM_010806237.2 , XM_015471690.1 , XM_015471690.2 , XM_015463801.1
    Accession Version XM_015463801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319287.1
    CDS219..3602
    Translation

    Target ORF information:

    RefSeq Version XM_015463801.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52841
    Clone ID Related Accession (Same CDS sequence) XM_010806238.1 , XM_015463803.1 , XM_010806238.2
    Accession Version XM_010806238.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGCT
    TACACAGAAT CGTTGTCACG TATTTTAATT CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA
    TCTGTGAAAA ATTCAGCTTC CCGTTCATTC CATTCTTCTC CTAAGAAGTC TCCAGTTCAC
    TCACTCCTGA CTAGTTCAGT TGAGGATTCC ATAGGTTCCT CACCACAATA TAGATCTACC
    AAACCTGTTC ATTCACCTGA TTCTGTTAAA GTCTCAGCCA ATAGAAACAA CCGGAAAGAC
    ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT CTGGTAGAAG ATTTACAGAT
    GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA AAGAGAATAC AGAAAGTGAA
    AGACCAGGAA AAAATGTTAC TAAAATGATA CATTGCTTGC CTATTCTTTA CAGAGAGATG
    ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804540.1
    CDS219..3590
    Translation

    Target ORF information:

    RefSeq Version XM_010806238.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGCT
    TACACAGAAT CGTTGTCACG TATTTTAATT CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA
    TCTGTGAAAA ATTCAGCTTC CCGTTCATTC CATTCTTCTC CTAAGAAGTC TCCAGTTCAC
    TCACTCCTGA CTAGTTCAGT TGAGGATTCC ATAGGTTCCT CACCACAATA TAGATCTACC
    AAACCTGTTC ATTCACCTGA TTCTGTTAAA GTCTCAGCCA ATAGAAACAA CCGGAAAGAC
    ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT CTGGTAGAAG ATTTACAGAT
    GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA AAGAGAATAC AGAAAGTGAA
    AGACCAGGAA AAAATGTTAC TAAAATGATA CATTGCTTGC CTATTCTTTA CAGAGAGATG
    ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52841
    Clone ID Related Accession (Same CDS sequence) XM_010806238.1 , XM_015463803.1 , XM_010806238.2
    Accession Version XM_015463803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGCT
    TACACAGAAT CGTTGTCACG TATTTTAATT CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA
    TCTGTGAAAA ATTCAGCTTC CCGTTCATTC CATTCTTCTC CTAAGAAGTC TCCAGTTCAC
    TCACTCCTGA CTAGTTCAGT TGAGGATTCC ATAGGTTCCT CACCACAATA TAGATCTACC
    AAACCTGTTC ATTCACCTGA TTCTGTTAAA GTCTCAGCCA ATAGAAACAA CCGGAAAGAC
    ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT CTGGTAGAAG ATTTACAGAT
    GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA AAGAGAATAC AGAAAGTGAA
    AGACCAGGAA AAAATGTTAC TAAAATGATA CATTGCTTGC CTATTCTTTA CAGAGAGATG
    ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319289.1
    CDS219..3590
    Translation

    Target ORF information:

    RefSeq Version XM_015463803.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGCT
    TACACAGAAT CGTTGTCACG TATTTTAATT CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA
    TCTGTGAAAA ATTCAGCTTC CCGTTCATTC CATTCTTCTC CTAAGAAGTC TCCAGTTCAC
    TCACTCCTGA CTAGTTCAGT TGAGGATTCC ATAGGTTCCT CACCACAATA TAGATCTACC
    AAACCTGTTC ATTCACCTGA TTCTGTTAAA GTCTCAGCCA ATAGAAACAA CCGGAAAGAC
    ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT CTGGTAGAAG ATTTACAGAT
    GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA AAGAGAATAC AGAAAGTGAA
    AGACCAGGAA AAAATGTTAC TAAAATGATA CATTGCTTGC CTATTCTTTA CAGAGAGATG
    ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52841
    Clone ID Related Accession (Same CDS sequence) XM_010806238.1 , XM_015463803.1 , XM_010806238.2
    Accession Version XM_010806238.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGCT
    TACACAGAAT CGTTGTCACG TATTTTAATT CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA
    TCTGTGAAAA ATTCAGCTTC CCGTTCATTC CATTCTTCTC CTAAGAAGTC TCCAGTTCAC
    TCACTCCTGA CTAGTTCAGT TGAGGATTCC ATAGGTTCCT CACCACAATA TAGATCTACC
    AAACCTGTTC ATTCACCTGA TTCTGTTAAA GTCTCAGCCA ATAGAAACAA CCGGAAAGAC
    ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT CTGGTAGAAG ATTTACAGAT
    GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA AAGAGAATAC AGAAAGTGAA
    AGACCAGGAA AAAATGTTAC TAAAATGATA CATTGCTTGC CTATTCTTTA CAGAGAGATG
    ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804540.1
    CDS240..3611
    Translation

    Target ORF information:

    RefSeq Version XM_010806238.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGTTTGC AGAATGTCAA
    GATATAATAC AGCGTCAGGA ACAAGAATCT GTGCGCCTAA AGCTTCAACA CACATTGGAT
    GTAAAAGAAC TGCAGGGCCC TGGATACACC TCAAATTCTG CTGTGAAACC ACGCCTTCTA
    CAACCATCAC CTGCTGCTCG TTCTCATTCC AATGTTCCAT CTTCCCAGTC TACAGCCAGC
    TTCCTGTCTC ATCACTCTAT GAAACCTAAC GCAAAGGAAG ATCCAACGAG GGACTTGAAA
    CAGCTTCTCC AGGAGTTGAG AAGTGTGATC AACGAGGAGC CAGCCGTGCC TCTGGGCAAG
    AGCGAAGAAG ACGGGAGAGC ACCATCTCTG GGGGTCTCCT TTGTGGCTGT AAGCAACAAC
    TCATTAAGGG AATCCACTGA AGGCAGCAAA TCAAGTGAAA CTTTTAGCAG AGAGCCAGTC
    ACGTTACACG TAGCAGAGTT GGAAGATCCG CCTGGTTGCT TTGTATTCCC ATCTGCAGCT
    TACACAGAAT CGTTGTCACG TATTTTAATT CTAAAGGTTT CTCAGGAGAC AGGAAGTCCA
    TCTGTGAAAA ATTCAGCTTC CCGTTCATTC CATTCTTCTC CTAAGAAGTC TCCAGTTCAC
    TCACTCCTGA CTAGTTCAGT TGAGGATTCC ATAGGTTCCT CACCACAATA TAGATCTACC
    AAACCTGTTC ATTCACCTGA TTCTGTTAAA GTCTCAGCCA ATAGAAACAA CCGGAAAGAC
    ATGCAGGAAG CTCCAAACCA GACTGGAAAG CTTGCAAACT CTGGTAGAAG ATTTACAGAT
    GAAGAACCAA GCAATGTCTT CAATGATCAG AAATCAAGAA AAGAGAATAC AGAAAGTGAA
    AGACCAGGAA AAAATGTTAC TAAAATGATA CATTGCTTGC CTATTCTTTA CAGAGAGATG
    ATGCCCGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52842
    Clone ID Related Accession (Same CDS sequence) XM_015463804.1 , XM_010806239.1 , XM_010806239.2
    Accession Version XM_015463804.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGCATC TTTGCAGTTT GCAGAATGTC AAGATATAAT ACAGCGTCAG
    GAACAAGAAT CTGTGCGCCT AAAGCTTCAA CACACATTGG ATGTAAAAGA ACTGCAGGGC
    CCTGGATACA CCTCAAATTC TGCTGTGAAA CCACGCCTTC TACAACCATC ACCTGCTGCT
    CGTTCTCATT CCAATGTTCC ATCTTCCCAG TCTACAGCCA GCTTCCTGTC TCATCACTCT
    ATGAAACCTA ACGCAAAGGA AGATCCAACG AGGGACTTGA AACAGCTTCT CCAGGAGTTG
    AGAAGTGTGA TCAACGAGGA GCCAGCCGTG CCTCTGGGCA AGAGCGAAGA AGACGGGAGA
    GCACCATCTC TGGGGGTCTC CTTTGTGGCT GTAAGCAACA ACTCATTAAG GGAATCCACT
    GAAGGCAGCA AATCAAGTGA AACTTTTAGC AGAGAGCCAG TCACGTTACA CGTAGCAGAG
    TTGGAAGATC CGCCTGGTTG CTTTGTATTC CCATCTGCAG CTTACACAGA ATCGTTGTCA
    CGTATTTTAA TTCTAAAGGT TTCTCAGGAG ACAGGAAGTC CATCTGTGAA AAATTCAGCT
    TCCCGTTCAT TCCATTCTTC TCCTAAGAAG TCTCCAGTTC ACTCACTCCT GACTAGTTCA
    GTTGAGGATT CCATAGGTTC CTCACCACAA TATAGATCTA CCAAACCTGT TCATTCACCT
    GATTCTGTTA AAGTCTCAGC CAATAGAAAC AACCGGAAAG ACATGCAGGA AGCTCCAAAC
    CAGACTGGAA AGCTTGCAAA CTCTGGTAGA AGATTTACAG ATGAAGAACC AAGCAATGTC
    TTCAATGATC AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT
    ACTAAAATGA TACATTGCTT GCCTATTCTT TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319290.1
    CDS219..3572
    Translation

    Target ORF information:

    RefSeq Version XM_015463804.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGCATC TTTGCAGTTT GCAGAATGTC AAGATATAAT ACAGCGTCAG
    GAACAAGAAT CTGTGCGCCT AAAGCTTCAA CACACATTGG ATGTAAAAGA ACTGCAGGGC
    CCTGGATACA CCTCAAATTC TGCTGTGAAA CCACGCCTTC TACAACCATC ACCTGCTGCT
    CGTTCTCATT CCAATGTTCC ATCTTCCCAG TCTACAGCCA GCTTCCTGTC TCATCACTCT
    ATGAAACCTA ACGCAAAGGA AGATCCAACG AGGGACTTGA AACAGCTTCT CCAGGAGTTG
    AGAAGTGTGA TCAACGAGGA GCCAGCCGTG CCTCTGGGCA AGAGCGAAGA AGACGGGAGA
    GCACCATCTC TGGGGGTCTC CTTTGTGGCT GTAAGCAACA ACTCATTAAG GGAATCCACT
    GAAGGCAGCA AATCAAGTGA AACTTTTAGC AGAGAGCCAG TCACGTTACA CGTAGCAGAG
    TTGGAAGATC CGCCTGGTTG CTTTGTATTC CCATCTGCAG CTTACACAGA ATCGTTGTCA
    CGTATTTTAA TTCTAAAGGT TTCTCAGGAG ACAGGAAGTC CATCTGTGAA AAATTCAGCT
    TCCCGTTCAT TCCATTCTTC TCCTAAGAAG TCTCCAGTTC ACTCACTCCT GACTAGTTCA
    GTTGAGGATT CCATAGGTTC CTCACCACAA TATAGATCTA CCAAACCTGT TCATTCACCT
    GATTCTGTTA AAGTCTCAGC CAATAGAAAC AACCGGAAAG ACATGCAGGA AGCTCCAAAC
    CAGACTGGAA AGCTTGCAAA CTCTGGTAGA AGATTTACAG ATGAAGAACC AAGCAATGTC
    TTCAATGATC AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT
    ACTAAAATGA TACATTGCTT GCCTATTCTT TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52842
    Clone ID Related Accession (Same CDS sequence) XM_015463804.1 , XM_010806239.1 , XM_010806239.2
    Accession Version XM_010806239.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGCATC TTTGCAGTTT GCAGAATGTC AAGATATAAT ACAGCGTCAG
    GAACAAGAAT CTGTGCGCCT AAAGCTTCAA CACACATTGG ATGTAAAAGA ACTGCAGGGC
    CCTGGATACA CCTCAAATTC TGCTGTGAAA CCACGCCTTC TACAACCATC ACCTGCTGCT
    CGTTCTCATT CCAATGTTCC ATCTTCCCAG TCTACAGCCA GCTTCCTGTC TCATCACTCT
    ATGAAACCTA ACGCAAAGGA AGATCCAACG AGGGACTTGA AACAGCTTCT CCAGGAGTTG
    AGAAGTGTGA TCAACGAGGA GCCAGCCGTG CCTCTGGGCA AGAGCGAAGA AGACGGGAGA
    GCACCATCTC TGGGGGTCTC CTTTGTGGCT GTAAGCAACA ACTCATTAAG GGAATCCACT
    GAAGGCAGCA AATCAAGTGA AACTTTTAGC AGAGAGCCAG TCACGTTACA CGTAGCAGAG
    TTGGAAGATC CGCCTGGTTG CTTTGTATTC CCATCTGCAG CTTACACAGA ATCGTTGTCA
    CGTATTTTAA TTCTAAAGGT TTCTCAGGAG ACAGGAAGTC CATCTGTGAA AAATTCAGCT
    TCCCGTTCAT TCCATTCTTC TCCTAAGAAG TCTCCAGTTC ACTCACTCCT GACTAGTTCA
    GTTGAGGATT CCATAGGTTC CTCACCACAA TATAGATCTA CCAAACCTGT TCATTCACCT
    GATTCTGTTA AAGTCTCAGC CAATAGAAAC AACCGGAAAG ACATGCAGGA AGCTCCAAAC
    CAGACTGGAA AGCTTGCAAA CTCTGGTAGA AGATTTACAG ATGAAGAACC AAGCAATGTC
    TTCAATGATC AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT
    ACTAAAATGA TACATTGCTT GCCTATTCTT TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804541.1
    CDS219..3572
    Translation

    Target ORF information:

    RefSeq Version XM_010806239.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGCATC TTTGCAGTTT GCAGAATGTC AAGATATAAT ACAGCGTCAG
    GAACAAGAAT CTGTGCGCCT AAAGCTTCAA CACACATTGG ATGTAAAAGA ACTGCAGGGC
    CCTGGATACA CCTCAAATTC TGCTGTGAAA CCACGCCTTC TACAACCATC ACCTGCTGCT
    CGTTCTCATT CCAATGTTCC ATCTTCCCAG TCTACAGCCA GCTTCCTGTC TCATCACTCT
    ATGAAACCTA ACGCAAAGGA AGATCCAACG AGGGACTTGA AACAGCTTCT CCAGGAGTTG
    AGAAGTGTGA TCAACGAGGA GCCAGCCGTG CCTCTGGGCA AGAGCGAAGA AGACGGGAGA
    GCACCATCTC TGGGGGTCTC CTTTGTGGCT GTAAGCAACA ACTCATTAAG GGAATCCACT
    GAAGGCAGCA AATCAAGTGA AACTTTTAGC AGAGAGCCAG TCACGTTACA CGTAGCAGAG
    TTGGAAGATC CGCCTGGTTG CTTTGTATTC CCATCTGCAG CTTACACAGA ATCGTTGTCA
    CGTATTTTAA TTCTAAAGGT TTCTCAGGAG ACAGGAAGTC CATCTGTGAA AAATTCAGCT
    TCCCGTTCAT TCCATTCTTC TCCTAAGAAG TCTCCAGTTC ACTCACTCCT GACTAGTTCA
    GTTGAGGATT CCATAGGTTC CTCACCACAA TATAGATCTA CCAAACCTGT TCATTCACCT
    GATTCTGTTA AAGTCTCAGC CAATAGAAAC AACCGGAAAG ACATGCAGGA AGCTCCAAAC
    CAGACTGGAA AGCTTGCAAA CTCTGGTAGA AGATTTACAG ATGAAGAACC AAGCAATGTC
    TTCAATGATC AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT
    ACTAAAATGA TACATTGCTT GCCTATTCTT TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52842
    Clone ID Related Accession (Same CDS sequence) XM_015463804.1 , XM_010806239.1 , XM_010806239.2
    Accession Version XM_010806239.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGCATC TTTGCAGTTT GCAGAATGTC AAGATATAAT ACAGCGTCAG
    GAACAAGAAT CTGTGCGCCT AAAGCTTCAA CACACATTGG ATGTAAAAGA ACTGCAGGGC
    CCTGGATACA CCTCAAATTC TGCTGTGAAA CCACGCCTTC TACAACCATC ACCTGCTGCT
    CGTTCTCATT CCAATGTTCC ATCTTCCCAG TCTACAGCCA GCTTCCTGTC TCATCACTCT
    ATGAAACCTA ACGCAAAGGA AGATCCAACG AGGGACTTGA AACAGCTTCT CCAGGAGTTG
    AGAAGTGTGA TCAACGAGGA GCCAGCCGTG CCTCTGGGCA AGAGCGAAGA AGACGGGAGA
    GCACCATCTC TGGGGGTCTC CTTTGTGGCT GTAAGCAACA ACTCATTAAG GGAATCCACT
    GAAGGCAGCA AATCAAGTGA AACTTTTAGC AGAGAGCCAG TCACGTTACA CGTAGCAGAG
    TTGGAAGATC CGCCTGGTTG CTTTGTATTC CCATCTGCAG CTTACACAGA ATCGTTGTCA
    CGTATTTTAA TTCTAAAGGT TTCTCAGGAG ACAGGAAGTC CATCTGTGAA AAATTCAGCT
    TCCCGTTCAT TCCATTCTTC TCCTAAGAAG TCTCCAGTTC ACTCACTCCT GACTAGTTCA
    GTTGAGGATT CCATAGGTTC CTCACCACAA TATAGATCTA CCAAACCTGT TCATTCACCT
    GATTCTGTTA AAGTCTCAGC CAATAGAAAC AACCGGAAAG ACATGCAGGA AGCTCCAAAC
    CAGACTGGAA AGCTTGCAAA CTCTGGTAGA AGATTTACAG ATGAAGAACC AAGCAATGTC
    TTCAATGATC AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT
    ACTAAAATGA TACATTGCTT GCCTATTCTT TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804541.1
    CDS240..3593
    Translation

    Target ORF information:

    RefSeq Version XM_010806239.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGCATC TTTGCAGTTT GCAGAATGTC AAGATATAAT ACAGCGTCAG
    GAACAAGAAT CTGTGCGCCT AAAGCTTCAA CACACATTGG ATGTAAAAGA ACTGCAGGGC
    CCTGGATACA CCTCAAATTC TGCTGTGAAA CCACGCCTTC TACAACCATC ACCTGCTGCT
    CGTTCTCATT CCAATGTTCC ATCTTCCCAG TCTACAGCCA GCTTCCTGTC TCATCACTCT
    ATGAAACCTA ACGCAAAGGA AGATCCAACG AGGGACTTGA AACAGCTTCT CCAGGAGTTG
    AGAAGTGTGA TCAACGAGGA GCCAGCCGTG CCTCTGGGCA AGAGCGAAGA AGACGGGAGA
    GCACCATCTC TGGGGGTCTC CTTTGTGGCT GTAAGCAACA ACTCATTAAG GGAATCCACT
    GAAGGCAGCA AATCAAGTGA AACTTTTAGC AGAGAGCCAG TCACGTTACA CGTAGCAGAG
    TTGGAAGATC CGCCTGGTTG CTTTGTATTC CCATCTGCAG CTTACACAGA ATCGTTGTCA
    CGTATTTTAA TTCTAAAGGT TTCTCAGGAG ACAGGAAGTC CATCTGTGAA AAATTCAGCT
    TCCCGTTCAT TCCATTCTTC TCCTAAGAAG TCTCCAGTTC ACTCACTCCT GACTAGTTCA
    GTTGAGGATT CCATAGGTTC CTCACCACAA TATAGATCTA CCAAACCTGT TCATTCACCT
    GATTCTGTTA AAGTCTCAGC CAATAGAAAC AACCGGAAAG ACATGCAGGA AGCTCCAAAC
    CAGACTGGAA AGCTTGCAAA CTCTGGTAGA AGATTTACAG ATGAAGAACC AAGCAATGTC
    TTCAATGATC AGAAATCAAG AAAAGAGAAT ACAGAAAGTG AAAGACCAGG AAAAAATGTT
    ACTAAAATGA TACATTGCTT GCCTATTCTT TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52843
    Clone ID Related Accession (Same CDS sequence) XM_015463805.1 , XM_010806241.3 , XM_010806241.2
    Accession Version XM_015463805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319291.1
    CDS219..3548
    Translation

    Target ORF information:

    RefSeq Version XM_015463805.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52843
    Clone ID Related Accession (Same CDS sequence) XM_015463805.1 , XM_010806241.3 , XM_010806241.2
    Accession Version XM_010806241.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804543.1
    CDS239..3568
    Translation

    Target ORF information:

    RefSeq Version XM_010806241.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806241.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52843
    Clone ID Related Accession (Same CDS sequence) XM_015463805.1 , XM_010806241.3 , XM_010806241.2
    Accession Version XM_010806241.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804543.1
    CDS219..3548
    Translation

    Target ORF information:

    RefSeq Version XM_010806241.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806241.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAAGCAACA ACTCATTAAG GGAATCCACT GAAGGCAGCA AATCAAGTGA AACTTTTAGC
    AGAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52844
    Clone ID Related Accession (Same CDS sequence) XM_015463806.1 , XM_010806242.2 , XM_010806242.3
    Accession Version XM_015463806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319292.1
    CDS219..3542
    Translation

    Target ORF information:

    RefSeq Version XM_015463806.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52844
    Clone ID Related Accession (Same CDS sequence) XM_015463806.1 , XM_010806242.2 , XM_010806242.3
    Accession Version XM_010806242.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804544.1
    CDS219..3542
    Translation

    Target ORF information:

    RefSeq Version XM_010806242.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806242.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52844
    Clone ID Related Accession (Same CDS sequence) XM_015463806.1 , XM_010806242.2 , XM_010806242.3
    Accession Version XM_010806242.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806242.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804544.1
    CDS239..3562
    Translation

    Target ORF information:

    RefSeq Version XM_010806242.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806242.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG CTTACACAGA ATCGTTGTCA CGTATTTTAA TTCTAAAGGT TTCTCAGGAG
    ACAGGAAGTC CATCTGTGAA AAATTCAGCT TCCCGTTCAT TCCATTCTTC TCCTAAGAAG
    TCTCCAGTTC ACTCACTCCT GACTAGTTCA GTTGAGGATT CCATAGGTTC CTCACCACAA
    TATAGATCTA CCAAACCTGT TCATTCACCT GATTCTGTTA AAGTCTCAGC CAATAGAAAC
    AACCGGAAAG ACATGCAGGA AGCTCCAAAC CAGACTGGAA AGCTTGCAAA CTCTGGTAGA
    AGATTTACAG ATGAAGAACC AAGCAATGTC TTCAATGATC AGAAATCAAG AAAAGAGAAT
    ACAGAAAGTG AAAGACCAGG AAAAAATGTT ACTAAAATGA TACATTGCTT GCCTATTCTT
    TACAGAGAGA TGATGCCCGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52845
    Clone ID Related Accession (Same CDS sequence) XM_015463807.1 , XM_010806244.2 , XM_010806244.3
    Accession Version XM_015463807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319293.1
    CDS219..3488
    Translation

    Target ORF information:

    RefSeq Version XM_015463807.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52845
    Clone ID Related Accession (Same CDS sequence) XM_015463807.1 , XM_010806244.2 , XM_010806244.3
    Accession Version XM_010806244.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804546.1
    CDS219..3488
    Translation

    Target ORF information:

    RefSeq Version XM_010806244.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806244.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52845
    Clone ID Related Accession (Same CDS sequence) XM_015463807.1 , XM_010806244.2 , XM_010806244.3
    Accession Version XM_010806244.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806244.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804546.1
    CDS239..3508
    Translation

    Target ORF information:

    RefSeq Version XM_010806244.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806244.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACAAA AACCTTGTGA ACCCAAAAAT GAACATCTTT TGCCAAGCAG TGGCATCCCA 
    TCCAATGCAG GTTCTTCAAG TCCATTTTTT ATGTCATCTA TTCGTGGAAC AATAATTGAA
    AACACATCTT CAACTGGGAC TATAACACAA ATTCCTTTTT TCCCTAAATA TGAAGTGGAA
    CTTGATTCTC ATAGAAAAGT CATTCCTTAC CCTGGAAAGG AACACATTGA GCGTGTTTTG
    GAAGAATATT CACATCAAGT CAAAGATTTG CAGAGAAGAC TAAATGAAAG CAATGAATTG
    CATGAGAAAC AAAAGTTTTA CTTAAGACAA TCAGTCATTG ATTTGCAAAC AAAACTTCAA
    GAGATGCAGA TGGAGAGAGA TGCCATGGCT GACATCAGAC GAAGGGAGAG TCAGTCCCAG
    GAGGATTTAC GAAATCAGCT TCAAAATACA GTTCATGAAC TTGAAGCTGC CAAATGCCTT
    AAGGACGATA TGCTGAAAGA TAGCAGCACA CAGATAGAGC AGCTAAGAAA GATGGTGCTT
    AGTCATGAGG GAGTGCTTCA AGAAATCCGA TCAGTCCTAG TTGACTTTGA AGAAGCCTCA
    GGCAAGAAAG TATTTGAACA TGACAGCATG TCTACCATCC ACTCCCGAAA CATGGCCTCA
    GCTATTAGTA AAGTCCTAAG AGAATTAGAC ACAGAGATTT CTTATCTTAA AGGGAGGATA
    TTTCCAGTAG AGGATCAGCT TGAAGCACTG AAATCTGAAT CACAAAACAA AATGGAACTA
    CTTCTTCAAC AACATCAAGA TCGGATTGAA CAGTTAATAA GTGAACATGA AGCTGAAATA
    ACAGGACTCA CCGAGAAAGC TAGCAGTGCT CGGAGCCAAG CCAACAGTAT CCAGAGTCAA
    TTGGAAATCA TTCAAGAGCA AGCAAGAAAC CAAAATTCAA TGTATATGCG CCAACTCAGT
    GACCTGGAAT CTACTGTTTC TCAACTACGT TCTGAATTAA GGGAAGCCAA AAGGATGTAT
    GAAGACAAGA TAGAAGAGCT GGAGAAGCAG TTAGTTCTCG CAAACTCAGA GCTAACTGAA
    GCACGGACGG AACGTGATCA GTTCAGTCAG GAATCTGGAA ATCTGGACGA CCAACTTCAA
    AAGCTCTTGG CCGACCTACA TAAACGAGAG AAGGAGCTGA GTCTGGAGAA GGAGCAGAAC
    AAGAGGCTGT GGGACCGGGA CACGGGCAAC AGCATCACCA TCGACCACCT GCGGCGGGAA
    CTGGACGACA GGAACATGGA GGTGCAGCGC CTGGAGGCCT TGCTCAAGGC CATGAAGAGC
    GAGTGTCAGG GCCAGATGGA GCGGCAGATG GCAGCCATTC AAGGAAAGAA TGAAAGTCTA
    GAAAAAGTGT CCTCCTTGAC TGCTCAGCTT GAATCCACCA AAGAGATGCT CCGCAAAGTG
    GTGGAAGAGT TGACAGCCAA GAAGATGACC CTGGAGAGTT CGGAGAGGAC CGTGTCGGAC
    CTGACAGCTT CCCTCCAGGA AAAGGAGCGA GCCATCGAGG CCACCAACGC AGAAATCACG
    AAGCTGCGCT CCCGGGTGGA CTTGAAGTTG CAGGAGCTGC AGCACCTGAA GAATGAAGGG
    GATCACCTCC GAAACGTGCA GACAGAGTGT GAGGCCCTCA GGCTGCAGAT GGCAGAGAAG
    GACAAAGTGA TCGAGATCCT GCGACAGCAG ATTGAGAACA TGACGCAGTT GGTGGGCCAG
    CACGGGCGGA CGGCCGGTGC TATGCAAGTG GAGAAAGCTC AGCTGGAGAA GGAGATCAAT
    GATAGGAGGC TGGAGCTACA GGAAGTTAAG ATTTTGAAAG ATAAAAGAGA TGCAAAGATA
    CGGGAGCTTG AAGCCAGAGT GAGTGACCTG GAACTGGAAA AGGTGAAGCT GGTGAACGCA
    GGCTCCGAGA GGCTCCGTGC AGTGAAAGAC ATCAAGCAAG AGAGAGACCA TTTATTAAGT
    GAGGTGAAAG CTAGTAGGAA TGAGTTAAAC AGTCTTACAG AGGACTATGA AGTCTTAAAA
    AGGAATTTTC GAAACAAAAG TGAAGAAATG GAAACTACTA CAAATAAGCT GAAAATGCAA
    TTAAAATCTG CACAGTCTGA GCTGGAGCAA ACAAGAAATA CATTAAAGTC AATGGAAGGA
    TCTGATGGTC ATGCTATGAA AGTGGCAATG GGGATGCAAA AACAAATTAC AGCTAAAAGA
    GGTCAGATAG ATGCCCTTCA GAGCAAGATA CAATTTTTGG AAGAGGCAAT GACAAATGCA
    AACAAGGAGA AGCATTTTCT AAAAGAAGAG AAGACAAAAT TAAGCCAGGA ATTGAGTACT
    GTTTCAACAG AGAAAAACAA GATGGCCGGA GAACTAGAAG TCCTGCGATC TCAGGAGCGC
    CGATTGAAAG AAAAGGTTGC TAATATGGAA GTTGCTCTTG ATAAGGCATC TTTGCAGTTT
    GCAGAATGTC AAGATATAAT ACAGCGTCAG GAACAAGAAT CTGTGCGCCT AAAGCTTCAA
    CACACATTGG ATGTAAAAGA ACTGCAGGGC CCTGGATACA CCTCAAATTC TGCTGTGAAA
    CCACGCCTTC TACAACCATC ACCTGCTGCT CGTTCTCATT CCAATGTTCC ATCTTCCCAG
    TCTACAGCCA GCTTCCTGTC TCATCACTCT ATGAAACCTA ACGCAAAGGA AGATCCAACG
    AGGGACTTGA AACAGCTTCT CCAGGAGTTG AGAAGTGTGA TCAACGAGGA GCCAGCCGTG
    CCTCTGGGCA AGAGCGAAGA AGACGGGAGA GCACCATCTC TGGGGGTCTC CTTTGTGGCT
    GTAGAGCCAG TCACGTTACA CGTAGCAGAG TTGGAAGATC CGCCTGGTTG CTTTGTATTC
    CCATCTGCAG GAAGTCCATC TGTGAAAAAT TCAGCTTCCC GTTCATTCCA TTCTTCTCCT
    AAGAAGTCTC CAGTTCACTC ACTCCTGACT AGTTCAGTTG AGGATTCCAT AGGTTCCTCA
    CCACAATATA GATCTACCAA ACCTGTTCAT TCACCTGATT CTGTTAAAGT CTCAGCCAAT
    AGAAACAACC GGAAAGACAT GCAGGAAGCT CCAAACCAGA CTGGAAAGCT TGCAAACTCT
    GGTAGAAGAT TTACAGATGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ATCAAGAAAA
    GAGAATACAG AAAGTGAAAG ACCAGGAAAA AATGTTACTA AAATGATACA TTGCTTGCCT
    ATTCTTTACA GAGAGATGAT GCCCGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52846
    Clone ID Related Accession (Same CDS sequence) XM_015463810.1 , XM_010806247.3 , XM_010806247.2 , XM_015471692.1 , XM_015463809.1
    Accession Version XM_015463810.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319296.1
    CDS158..3178
    Translation

    Target ORF information:

    RefSeq Version XM_015463810.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463810.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52846
    Clone ID Related Accession (Same CDS sequence) XM_015463810.1 , XM_010806247.3 , XM_010806247.2 , XM_015471692.1 , XM_015463809.1
    Accession Version XM_010806247.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804549.1
    CDS240..3260
    Translation

    Target ORF information:

    RefSeq Version XM_010806247.3
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806247.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52846
    Clone ID Related Accession (Same CDS sequence) XM_015463810.1 , XM_010806247.3 , XM_010806247.2 , XM_015471692.1 , XM_015463809.1
    Accession Version XM_010806247.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804549.1
    CDS220..3240
    Translation

    Target ORF information:

    RefSeq Version XM_010806247.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52846
    Clone ID Related Accession (Same CDS sequence) XM_015463810.1 , XM_010806247.3 , XM_010806247.2 , XM_015471692.1 , XM_015463809.1
    Accession Version XM_015471692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327178.1
    CDS158..3178
    Translation

    Target ORF information:

    RefSeq Version XM_015471692.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52846
    Clone ID Related Accession (Same CDS sequence) XM_015463810.1 , XM_010806247.3 , XM_010806247.2 , XM_015471692.1 , XM_015463809.1
    Accession Version XM_015463809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 158 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644952.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319295.1
    CDS220..3240
    Translation

    Target ORF information:

    RefSeq Version XM_015463809.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 158 (CCDC158), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAGTCA GTCCCAGGAG 
    GATTTACGAA ATCAGCTTCA AAATACAGTT CATGAACTTG AAGCTGCCAA ATGCCTTAAG
    GACGATATGC TGAAAGATAG CAGCACACAG ATAGAGCAGC TAAGAAAGAT GGTGCTTAGT
    CATGAGGGAG TGCTTCAAGA AATCCGATCA GTCCTAGTTG ACTTTGAAGA AGCCTCAGGC
    AAGAAAGTAT TTGAACATGA CAGCATGTCT ACCATCCACT CCCGAAACAT GGCCTCAGCT
    ATTAGTAAAG TCCTAAGAGA ATTAGACACA GAGATTTCTT ATCTTAAAGG GAGGATATTT
    CCAGTAGAGG ATCAGCTTGA AGCACTGAAA TCTGAATCAC AAAACAAAAT GGAACTACTT
    CTTCAACAAC ATCAAGATCG GATTGAACAG TTAATAAGTG AACATGAAGC TGAAATAACA
    GGACTCACCG AGAAAGCTAG CAGTGCTCGG AGCCAAGCCA ACAGTATCCA GAGTCAATTG
    GAAATCATTC AAGAGCAAGC AAGAAACCAA AATTCAATGT ATATGCGCCA ACTCAGTGAC
    CTGGAATCTA CTGTTTCTCA ACTACGTTCT GAATTAAGGG AAGCCAAAAG GATGTATGAA
    GACAAGATAG AAGAGCTGGA GAAGCAGTTA GTTCTCGCAA ACTCAGAGCT AACTGAAGCA
    CGGACGGAAC GTGATCAGTT CAGTCAGGAA TCTGGAAATC TGGACGACCA ACTTCAAAAG
    CTCTTGGCCG ACCTACATAA ACGAGAGAAG GAGCTGAGTC TGGAGAAGGA GCAGAACAAG
    AGGCTGTGGG ACCGGGACAC GGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAACTG
    GACGACAGGA ACATGGAGGT GCAGCGCCTG GAGGCCTTGC TCAAGGCCAT GAAGAGCGAG
    TGTCAGGGCC AGATGGAGCG GCAGATGGCA GCCATTCAAG GAAAGAATGA AAGTCTAGAA
    AAAGTGTCCT CCTTGACTGC TCAGCTTGAA TCCACCAAAG AGATGCTCCG CAAAGTGGTG
    GAAGAGTTGA CAGCCAAGAA GATGACCCTG GAGAGTTCGG AGAGGACCGT GTCGGACCTG
    ACAGCTTCCC TCCAGGAAAA GGAGCGAGCC ATCGAGGCCA CCAACGCAGA AATCACGAAG
    CTGCGCTCCC GGGTGGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT
    CACCTCCGAA ACGTGCAGAC AGAGTGTGAG GCCCTCAGGC TGCAGATGGC AGAGAAGGAC
    AAAGTGATCG AGATCCTGCG ACAGCAGATT GAGAACATGA CGCAGTTGGT GGGCCAGCAC
    GGGCGGACGG CCGGTGCTAT GCAAGTGGAG AAAGCTCAGC TGGAGAAGGA GATCAATGAT
    AGGAGGCTGG AGCTACAGGA AGTTAAGATT TTGAAAGATA AAAGAGATGC AAAGATACGG
    GAGCTTGAAG CCAGAGTGAG TGACCTGGAA CTGGAAAAGG TGAAGCTGGT GAACGCAGGC
    TCCGAGAGGC TCCGTGCAGT GAAAGACATC AAGCAAGAGA GAGACCATTT ATTAAGTGAG
    GTGAAAGCTA GTAGGAATGA GTTAAACAGT CTTACAGAGG ACTATGAAGT CTTAAAAAGG
    AATTTTCGAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAGCTGAA AATGCAATTA
    AAATCTGCAC AGTCTGAGCT GGAGCAAACA AGAAATACAT TAAAGTCAAT GGAAGGATCT
    GATGGTCATG CTATGAAAGT GGCAATGGGG ATGCAAAAAC AAATTACAGC TAAAAGAGGT
    CAGATAGATG CCCTTCAGAG CAAGATACAA TTTTTGGAAG AGGCAATGAC AAATGCAAAC
    AAGGAGAAGC ATTTTCTAAA AGAAGAGAAG ACAAAATTAA GCCAGGAATT GAGTACTGTT
    TCAACAGAGA AAAACAAGAT GGCCGGAGAA CTAGAAGTCC TGCGATCTCA GGAGCGCCGA
    TTGAAAGAAA AGGTTGCTAA TATGGAAGTT GCTCTTGATA AGGCATCTTT GCAGTTTGCA
    GAATGTCAAG ATATAATACA GCGTCAGGAA CAAGAATCTG TGCGCCTAAA GCTTCAACAC
    ACATTGGATG TAAAAGAACT GCAGGGCCCT GGATACACCT CAAATTCTGC TGTGAAACCA
    CGCCTTCTAC AACCATCACC TGCTGCTCGT TCTCATTCCA ATGTTCCATC TTCCCAGTCT
    ACAGCCAGCT TCCTGTCTCA TCACTCTATG AAACCTAACG CAAAGGAAGA TCCAACGAGG
    GACTTGAAAC AGCTTCTCCA GGAGTTGAGA AGTGTGATCA ACGAGGAGCC AGCCGTGCCT
    CTGGGCAAGA GCGAAGAAGA CGGGAGAGCA CCATCTCTGG GGGTCTCCTT TGTGGCTGTA
    AGCAACAACT CATTAAGGGA ATCCACTGAA GGCAGCAAAT CAAGTGAAAC TTTTAGCAGA
    GAGCCAGTCA CGTTACACGT AGCAGAGTTG GAAGATCCGC CTGGTTGCTT TGTATTCCCA
    TCTGCAGCTT ACACAGAATC GTTGTCACGT ATTTTAATTC TAAAGGTTTC TCAGGAGACA
    GGAAGTCCAT CTGTGAAAAA TTCAGCTTCC CGTTCATTCC ATTCTTCTCC TAAGAAGTCT
    CCAGTTCACT CACTCCTGAC TAGTTCAGTT GAGGATTCCA TAGGTTCCTC ACCACAATAT
    AGATCTACCA AACCTGTTCA TTCACCTGAT TCTGTTAAAG TCTCAGCCAA TAGAAACAAC
    CGGAAAGACA TGCAGGAAGC TCCAAACCAG ACTGGAAAGC TTGCAAACTC TGGTAGAAGA
    TTTACAGATG AAGAACCAAG CAATGTCTTC AATGATCAGA AATCAAGAAA AGAGAATACA
    GAAAGTGAAA GACCAGGAAA AAATGTTACT AAAATGATAC ATTGCTTGCC TATTCTTTAC
    AGAGAGATGA TGCCCGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.