RNF216 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF216
Entrez Gene ID	534455
Full Name	ring finger protein 216
Synonyms	TRIAD3
General protein information	Preferred Names ring finger protein 216 Names E3 ubiquitin-protein ligase RNF216
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005225169.3	XP_005225226.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_010819581.2	XP_010817883.1	E3 ubiquitin-protein ligase RNF216 isoform X2
NM_001193258.1	NP_001180187.1	E3 ubiquitin-protein ligase RNF216
XM_024984994.1	XP_024840762.1	E3 ubiquitin-protein ligase RNF216 isoform X2
XM_005225169.4	XP_005225226.1	E3 ubiquitin-protein ligase RNF216 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rnf216	NP_996993.1
Xenopus tropicalis (tropical clawed frog)	rnf216	NP_001016834.1
Rattus norvegicus (Norway rat)	Rnf216	NP_001100592.1
	RNF216	XP_002803244.1
Danio rerio (zebrafish)	rnf216	XP_687871.3
Homo sapiens (human)	RNF216	NP_996994.1
Bos taurus (cattle)	RNF216	NP_001180187.1
Pan troglodytes (chimpanzee)	RNF216	XP_518955.2
Canis lupus familiaris (dog)	RNF216	XP_536887.2
Gallus gallus (chicken)	RNF216	XP_414793.4

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RNF216 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF216 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa38444	NM_001193258.1 Latest version!	Bos taurus ring finger protein 216 (RNF216), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa33285	XM_005225169.4 Latest version!	Bos taurus ring finger protein 216 (RNF216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa52741	XM_010819581.2 Latest version!	Bos taurus ring finger protein 216 (RNF216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa230338	XM_024984994.1 Latest version!	Bos taurus ring finger protein 216 (RNF216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBa33285	XM_005225169.3	Bos taurus ring finger protein 216 (RNF216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF216 ( NM_001193258.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180187.1 Bos taurus ring finger protein 216 (RNF216), mRNA.

Price & Availability↑

CloneID	OBa38444
Clone ID Related Accession (Same CDS sequence)	NM_001193258.1
Accession Version	NM_001193258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF216
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Jul 17, 2010 this sequence version replaced XM_883230.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.1621.1, SRR5571259.35928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAGA TGATTCTGAG GATGACTACA GTGAATTTCT AGATCTTGGG 
CCTCCTGGAG TTTCTGGATT CATGAAGCCA AGTGGCCAAA CAGAAAGAGA ACCTAAGCCT 
GGACCAAGTC ACAACCAAGC AACCAATGAC TTCATCAACC CCAGATCAGA GCAGAAAGTC 
ATTATCTTAA AAGAAGGTAA TCTTTTTCCA GAAGCTGATC CTTTGGACAC TCAGAACCAG 
TCATCTGAAG AGTCAGAGAC AGAGCTTTTA TCAAACCTCG GAGAATCATC TGATATCCTC 
GATGACCAGG CCATCGAAGA AGACTGCTGG TTAGACCACT CTTACTTCCA GTCTCTGAAC 
CAGCAGCCCC GTGAGGTACC AAGCCAGGTT GTACCTCAGG AGCGGCGTCC AGAAGCAGAG 
CTGGGCCCAC TGCTGTATCA GCATGAGCCC CGGGGGTCAG CTTTTCCAAG ACCGGCGTTT 
GCAAGACCAG AGCCCCAGCA GGATTGGATT CCAGACCCTG CTTCTCCTCG GCCTGCCCAT 
CCTCTGGGAG AGCTTGAAGA CCAACAACTA GCAATAGATG ATGAAGAACC AGGCCCAGCA 
TTTCCGCTAG AAGAAACTCG GCAGCCCAAT TTGGGAAACC TGTGGGGGCA AGAAGCTTCT 
TACGTAGACC GGGAACTCAT TGCACTGTTG GTGAAAGAGA CAGAAGTAAG ATTTCCTGAT 
GTAGCAAATG GGTATGTTGA GGAAATAATT CATTTGAAGA ATTACTATGA TTTGAATGTA 
CTTTGTAATT TTCTTCTGGA AAATCCAGAT TATCCAAAGA GAGAAGACAG AGTCCTTATG 
AATCCCAGTA GCAGCCTGCT CGCCAGCCAG GATGAGATGA AGTTGCCTAC AGTAGACTTT 
TTTGACTATT CTAAATTAGC TCCTCTTGAC CAGCACTGCT TCATCCAAGC TGCCGACCTC 
CTGATGGCCG ACTTCAAAAT GCTTAGCAGT CAGGACATCA AGTGGGCCCT GCACGAGCTC 
AAAGGACACT ATGCAATCAC CCGAAAGGCC TTTTCTGACG CCATTAAAAA ATGGCAGGAG 
CTATCGCCAG AAACCAGTGG AAAAAGAAAA AAGAGAAAAG AAATGAATCA GTATTCTTAC 
ATAGATTTCA AATTTGAACA AGGTGACAAA AAAATAGAGA AGAGGATGTT CTTTCTGGAA 
AATAAGCGGC GACATTGTAG GTCCTATGAC CGGCGTGCCC TCCTTCCAGC TGTGCAGCAG 
GAGCAGGAGT TCTACGAGCA GAAAATCAAA GAGATGGCAG AGCATGAAGA CTTTTTGCTT 
GCCCTGCAGA TGAATGAAGA ACAGTATCAA AAGGATGGCC AGCTGATTGA GTGCCGCTGC 
TGCTATGGGG AGTTCCCATT CGAGGAGCTG ACGCAGTGTG CTGACGCTCA CTTATTCTGC 
AAAGAGTGTC TCATCAGATA CGCCCAAGAG GCAGTCTTTG GATCTGGAAA GTCAGAGCTC 
AGCTGTATGG AGGGCAGCTG CACATGCTCA TTCCCAGCCA GTGAACTGGA GAAGGTGCTT 
CCCCAGACCA TCCTGTATAA ATACTATGAG CGAAAAGCAG AGGAAGAAGT TGCAGCAGCC 
TACGCTGATG AGCTTGTCAG GTGCCCCTCC TGCAGCTTTC CTGCTCTCCT GGACAGTGAC 
GTGAAGAGGT TCAGTTGTCC CAATCCTCGC TGTCGAAAGG AAACCTGTAG GAAGTGTCAA 
GGACTCTGGA AAGAGCACAA CGGCCTCACT TGTGAGGAGC TGGCTGAAAA AGATGACATC 
AAGTACCGAA CATCTATTGA AGAAAAAATG ACTGCTGCCC GCATTAGGAA ATGCCACAAG 
TGTGGGACCA GCCTCATCAA ATCTGAAGGC TGCAACCGCA TGTCCTGCCG CTGCGGTGCC 
CAGATGTGCT ACCTCTGCCG CGTGTCCATC AATGGCTACG ACCACTTCTG CCAGCACCCT 
CGCTCCCCAG GAGCCCCCTG CCAGGAGTGT TCCCGATGCT CTCTCTGGAC CGACCCCACT 
GAAGATGATG AGAAGCTAAT TGAGGAAATC CAGAAGGAGG CCGAAGAGGA ACAGAAAAGA 
AAGAATGGAG AGAACACCTT CAAACGCATC GGCCCCCCCT TGGAGAAGCC CCCTGAGAAA 
GTGCAAAGGA CCGAGGCCCT GCCGAGGCCC GTGCCCCAGA ACCTGCACCA GCCGCAGATA 
CCCGCCTACG CCTTCGTGCA CCCACCCTTC CCCCTGCCGC CCGTCCGGCC CGTGTTCAAC 
AACTTCCCCC TCAACATGGG CCCCATCCCG GCACCCTACG TGCCGCCCCT GCCCAATATG 
CGCGTCAACT ACGAATTTGC CCCCATCCAC CTGCCCCTGG AACACAACCT GCCCATGCAC 
TTTGGCCCCC AGCCTCGGCA TCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180187.1
CDS	187..2796
Translation	MAEGNSSEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAEDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDFINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPLLYQHEPRGSAFPRPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETRQPNLGNLWGQEASYVDRELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFVHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	NM_001193258.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 216 (RNF216), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193258.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAGA TGATTCTGAG GATGACTACA GTGAATTTCT AGATCTTGGG 
CCTCCTGGAG TTTCTGGATT CATGAAGCCA AGTGGCCAAA CAGAAAGAGA ACCTAAGCCT 
GGACCAAGTC ACAACCAAGC AACCAATGAC TTCATCAACC CCAGATCAGA GCAGAAAGTC 
ATTATCTTAA AAGAAGGTAA TCTTTTTCCA GAAGCTGATC CTTTGGACAC TCAGAACCAG 
TCATCTGAAG AGTCAGAGAC AGAGCTTTTA TCAAACCTCG GAGAATCATC TGATATCCTC 
GATGACCAGG CCATCGAAGA AGACTGCTGG TTAGACCACT CTTACTTCCA GTCTCTGAAC 
CAGCAGCCCC GTGAGGTACC AAGCCAGGTT GTACCTCAGG AGCGGCGTCC AGAAGCAGAG 
CTGGGCCCAC TGCTGTATCA GCATGAGCCC CGGGGGTCAG CTTTTCCAAG ACCGGCGTTT 
GCAAGACCAG AGCCCCAGCA GGATTGGATT CCAGACCCTG CTTCTCCTCG GCCTGCCCAT 
CCTCTGGGAG AGCTTGAAGA CCAACAACTA GCAATAGATG ATGAAGAACC AGGCCCAGCA 
TTTCCGCTAG AAGAAACTCG GCAGCCCAAT TTGGGAAACC TGTGGGGGCA AGAAGCTTCT 
TACGTAGACC GGGAACTCAT TGCACTGTTG GTGAAAGAGA CAGAAGTAAG ATTTCCTGAT 
GTAGCAAATG GGTATGTTGA GGAAATAATT CATTTGAAGA ATTACTATGA TTTGAATGTA 
CTTTGTAATT TTCTTCTGGA AAATCCAGAT TATCCAAAGA GAGAAGACAG AGTCCTTATG 
AATCCCAGTA GCAGCCTGCT CGCCAGCCAG GATGAGATGA AGTTGCCTAC AGTAGACTTT 
TTTGACTATT CTAAATTAGC TCCTCTTGAC CAGCACTGCT TCATCCAAGC TGCCGACCTC 
CTGATGGCCG ACTTCAAAAT GCTTAGCAGT CAGGACATCA AGTGGGCCCT GCACGAGCTC 
AAAGGACACT ATGCAATCAC CCGAAAGGCC TTTTCTGACG CCATTAAAAA ATGGCAGGAG 
CTATCGCCAG AAACCAGTGG AAAAAGAAAA AAGAGAAAAG AAATGAATCA GTATTCTTAC 
ATAGATTTCA AATTTGAACA AGGTGACAAA AAAATAGAGA AGAGGATGTT CTTTCTGGAA 
AATAAGCGGC GACATTGTAG GTCCTATGAC CGGCGTGCCC TCCTTCCAGC TGTGCAGCAG 
GAGCAGGAGT TCTACGAGCA GAAAATCAAA GAGATGGCAG AGCATGAAGA CTTTTTGCTT 
GCCCTGCAGA TGAATGAAGA ACAGTATCAA AAGGATGGCC AGCTGATTGA GTGCCGCTGC 
TGCTATGGGG AGTTCCCATT CGAGGAGCTG ACGCAGTGTG CTGACGCTCA CTTATTCTGC 
AAAGAGTGTC TCATCAGATA CGCCCAAGAG GCAGTCTTTG GATCTGGAAA GTCAGAGCTC 
AGCTGTATGG AGGGCAGCTG CACATGCTCA TTCCCAGCCA GTGAACTGGA GAAGGTGCTT 
CCCCAGACCA TCCTGTATAA ATACTATGAG CGAAAAGCAG AGGAAGAAGT TGCAGCAGCC 
TACGCTGATG AGCTTGTCAG GTGCCCCTCC TGCAGCTTTC CTGCTCTCCT GGACAGTGAC 
GTGAAGAGGT TCAGTTGTCC CAATCCTCGC TGTCGAAAGG AAACCTGTAG GAAGTGTCAA 
GGACTCTGGA AAGAGCACAA CGGCCTCACT TGTGAGGAGC TGGCTGAAAA AGATGACATC 
AAGTACCGAA CATCTATTGA AGAAAAAATG ACTGCTGCCC GCATTAGGAA ATGCCACAAG 
TGTGGGACCA GCCTCATCAA ATCTGAAGGC TGCAACCGCA TGTCCTGCCG CTGCGGTGCC 
CAGATGTGCT ACCTCTGCCG CGTGTCCATC AATGGCTACG ACCACTTCTG CCAGCACCCT 
CGCTCCCCAG GAGCCCCCTG CCAGGAGTGT TCCCGATGCT CTCTCTGGAC CGACCCCACT 
GAAGATGATG AGAAGCTAAT TGAGGAAATC CAGAAGGAGG CCGAAGAGGA ACAGAAAAGA 
AAGAATGGAG AGAACACCTT CAAACGCATC GGCCCCCCCT TGGAGAAGCC CCCTGAGAAA 
GTGCAAAGGA CCGAGGCCCT GCCGAGGCCC GTGCCCCAGA ACCTGCACCA GCCGCAGATA 
CCCGCCTACG CCTTCGTGCA CCCACCCTTC CCCCTGCCGC CCGTCCGGCC CGTGTTCAAC 
AACTTCCCCC TCAACATGGG CCCCATCCCG GCACCCTACG TGCCGCCCCT GCCCAATATG 
CGCGTCAACT ACGAATTTGC CCCCATCCAC CTGCCCCTGG AACACAACCT GCCCATGCAC 
TTTGGCCCCC AGCCTCGGCA TCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_005225169.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225226.1 Bos taurus ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa33285
Clone ID Related Accession (Same CDS sequence)	XM_005225169.3 , XM_005225169.4
Accession Version	XM_005225169.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CTTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
CTGCTGTATC AGCATGAGCC CCGGGGGTCA GCTTTTCCAA GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAACAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGCAGCCCAA TTTGGGAAAC CTGTGGGGGC AAGAAGCTTC TTACGTAGAC 
CGGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA GGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGACGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGTATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ACGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGCCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAAAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCGTGC ACCCACCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAACACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225226.1
CDS	208..2988
Translation	MAEGNSSEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDFINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPLLYQHEPRGSAFPRPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETRQPNLGNLWGQEASYVDRELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFVHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_005225169.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225169.3

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CTTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
CTGCTGTATC AGCATGAGCC CCGGGGGTCA GCTTTTCCAA GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAACAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGCAGCCCAA TTTGGGAAAC CTGTGGGGGC AAGAAGCTTC TTACGTAGAC 
CGGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA GGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGACGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGTATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ACGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGCCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAAAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCGTGC ACCCACCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAACACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_005225169.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225226.1 Bos taurus ring finger protein 216 (RNF216), transcript variant X1, mRNA.

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CloneID	OBa33285
Clone ID Related Accession (Same CDS sequence)	XM_005225169.3 , XM_005225169.4
Accession Version	XM_005225169.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225169.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CTTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
CTGCTGTATC AGCATGAGCC CCGGGGGTCA GCTTTTCCAA GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAACAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGCAGCCCAA TTTGGGAAAC CTGTGGGGGC AAGAAGCTTC TTACGTAGAC 
CGGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA GGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGACGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGTATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ACGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGCCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAAAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCGTGC ACCCACCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAACACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225226.1
CDS	218..2998
Translation	MAEGNSSEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDFINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPLLYQHEPRGSAFPRPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETRQPNLGNLWGQEASYVDRELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFVHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_005225169.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225169.4

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CTTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
CTGCTGTATC AGCATGAGCC CCGGGGGTCA GCTTTTCCAA GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAACAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGCAGCCCAA TTTGGGAAAC CTGTGGGGGC AAGAAGCTTC TTACGTAGAC 
CGGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA GGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGACGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGTATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ACGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGCCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAAAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCGTGC ACCCACCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAACACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_010819581.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817883.1 Bos taurus ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa52741
Clone ID Related Accession (Same CDS sequence)	XM_010819581.2
Accession Version	XM_010819581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCAA GTGGCCAAAC AGAAAGAGAA CCTAAGCCTG GACCAAGTCA CAACCAAGCA 
ACCAATGACT TCATCAACCC CAGATCAGAG CAGAAAGTCA TTATCTTAAA AGAAGGTAAT 
CTTTTTCCAG AAGCTGATCC TTTGGACACT CAGAACCAGT CATCTGAAGA GTCAGAGACA 
GAGCTTTTAT CAAACCTCGG AGAATCATCT GATATCCTCG ATGACCAGGC CATCGAAGAA 
GACTGCTGGT TAGACCACTC TTACTTCCAG TCTCTGAACC AGCAGCCCCG TGAGGTACCA 
AGCCAGGTTG TACCTCAGGA GCGGCGTCCA GAAGCAGAGC TGGGCCCACT GCTGTATCAG 
CATGAGCCCC GGGGGTCAGC TTTTCCAAGA CCGGCGTTTG CAAGACCAGA GCCCCAGCAG 
GATTGGATTC CAGACCCTGC TTCTCCTCGG CCTGCCCATC CTCTGGGAGA GCTTGAAGAC 
CAACAACTAG CAATAGATGA TGAAGAACCA GGCCCAGCAT TTCCGCTAGA AGAAACTCGG 
CAGCCCAATT TGGGAAACCT GTGGGGGCAA GAAGCTTCTT ACGTAGACCG GGAACTCATT 
GCACTGTTGG TGAAAGAGAC AGAAGTAAGA TTTCCTGATG TAGCAAATGG GTATGTTGAG 
GAAATAATTC ATTTGAAGAA TTACTATGAT TTGAATGTAC TTTGTAATTT TCTTCTGGAA 
AATCCAGATT ATCCAAAGAG AGAAGACAGA GTCCTTATGA ATCCCAGTAG CAGCCTGCTC 
GCCAGCCAGG ATGAGATGAA GTTGCCTACA GTAGACTTTT TTGACTATTC TAAATTAGCT 
CCTCTTGACC AGCACTGCTT CATCCAAGCT GCCGACCTCC TGATGGCCGA CTTCAAAATG 
CTTAGCAGTC AGGACATCAA GTGGGCCCTG CACGAGCTCA AAGGACACTA TGCAATCACC 
CGAAAGGCCT TTTCTGACGC CATTAAAAAA TGGCAGGAGC TATCGCCAGA AACCAGTGGA 
AAAAGAAAAA AGAGAAAAGA AATGAATCAG TATTCTTACA TAGATTTCAA ATTTGAACAA 
GGTGACAAAA AAATAGAGAA GAGGATGTTC TTTCTGGAAA ATAAGCGGCG ACATTGTAGG 
TCCTATGACC GGCGTGCCCT CCTTCCAGCT GTGCAGCAGG AGCAGGAGTT CTACGAGCAG 
AAAATCAAAG AGATGGCAGA GCATGAAGAC TTTTTGCTTG CCCTGCAGAT GAATGAAGAA 
CAGTATCAAA AGGATGGCCA GCTGATTGAG TGCCGCTGCT GCTATGGGGA GTTCCCATTC 
GAGGAGCTGA CGCAGTGTGC TGACGCTCAC TTATTCTGCA AAGAGTGTCT CATCAGATAC 
GCCCAAGAGG CAGTCTTTGG ATCTGGAAAG TCAGAGCTCA GCTGTATGGA GGGCAGCTGC 
ACATGCTCAT TCCCAGCCAG TGAACTGGAG AAGGTGCTTC CCCAGACCAT CCTGTATAAA 
TACTATGAGC GAAAAGCAGA GGAAGAAGTT GCAGCAGCCT ACGCTGATGA GCTTGTCAGG 
TGCCCCTCCT GCAGCTTTCC TGCTCTCCTG GACAGTGACG TGAAGAGGTT CAGTTGTCCC 
AATCCTCGCT GTCGAAAGGA AACCTGTAGG AAGTGTCAAG GACTCTGGAA AGAGCACAAC 
GGCCTCACTT GTGAGGAGCT GGCTGAAAAA GATGACATCA AGTACCGAAC ATCTATTGAA 
GAAAAAATGA CTGCTGCCCG CATTAGGAAA TGCCACAAGT GTGGGACCAG CCTCATCAAA 
TCTGAAGGCT GCAACCGCAT GTCCTGCCGC TGCGGTGCCC AGATGTGCTA CCTCTGCCGC 
GTGTCCATCA ATGGCTACGA CCACTTCTGC CAGCACCCTC GCTCCCCAGG AGCCCCCTGC 
CAGGAGTGTT CCCGATGCTC TCTCTGGACC GACCCCACTG AAGATGATGA GAAGCTAATT 
GAGGAAATCC AGAAGGAGGC CGAAGAGGAA CAGAAAAGAA AGAATGGAGA GAACACCTTC 
AAACGCATCG GCCCCCCCTT GGAGAAGCCC CCTGAGAAAG TGCAAAGGAC CGAGGCCCTG 
CCGAGGCCCG TGCCCCAGAA CCTGCACCAG CCGCAGATAC CCGCCTACGC CTTCGTGCAC 
CCACCCTTCC CCCTGCCGCC CGTCCGGCCC GTGTTCAACA ACTTCCCCCT CAACATGGGC 
CCCATCCCGG CACCCTACGT GCCGCCCCTG CCCAATATGC GCGTCAACTA CGAATTTGCC 
CCCATCCACC TGCCCCTGGA ACACAACCTG CCCATGCACT TTGGCCCCCA GCCTCGGCAT 
CGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817883.1
CDS	313..2661
Translation	MKPSGQTEREPKPGPSHNQATNDFINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPLLYQHEPRGSAFPRPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETRQPNLGNLWGQEASYVDRELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFVHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_010819581.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819581.2

ORF Insert Sequence:

ATGAAGCCAA GTGGCCAAAC AGAAAGAGAA CCTAAGCCTG GACCAAGTCA CAACCAAGCA 
ACCAATGACT TCATCAACCC CAGATCAGAG CAGAAAGTCA TTATCTTAAA AGAAGGTAAT 
CTTTTTCCAG AAGCTGATCC TTTGGACACT CAGAACCAGT CATCTGAAGA GTCAGAGACA 
GAGCTTTTAT CAAACCTCGG AGAATCATCT GATATCCTCG ATGACCAGGC CATCGAAGAA 
GACTGCTGGT TAGACCACTC TTACTTCCAG TCTCTGAACC AGCAGCCCCG TGAGGTACCA 
AGCCAGGTTG TACCTCAGGA GCGGCGTCCA GAAGCAGAGC TGGGCCCACT GCTGTATCAG 
CATGAGCCCC GGGGGTCAGC TTTTCCAAGA CCGGCGTTTG CAAGACCAGA GCCCCAGCAG 
GATTGGATTC CAGACCCTGC TTCTCCTCGG CCTGCCCATC CTCTGGGAGA GCTTGAAGAC 
CAACAACTAG CAATAGATGA TGAAGAACCA GGCCCAGCAT TTCCGCTAGA AGAAACTCGG 
CAGCCCAATT TGGGAAACCT GTGGGGGCAA GAAGCTTCTT ACGTAGACCG GGAACTCATT 
GCACTGTTGG TGAAAGAGAC AGAAGTAAGA TTTCCTGATG TAGCAAATGG GTATGTTGAG 
GAAATAATTC ATTTGAAGAA TTACTATGAT TTGAATGTAC TTTGTAATTT TCTTCTGGAA 
AATCCAGATT ATCCAAAGAG AGAAGACAGA GTCCTTATGA ATCCCAGTAG CAGCCTGCTC 
GCCAGCCAGG ATGAGATGAA GTTGCCTACA GTAGACTTTT TTGACTATTC TAAATTAGCT 
CCTCTTGACC AGCACTGCTT CATCCAAGCT GCCGACCTCC TGATGGCCGA CTTCAAAATG 
CTTAGCAGTC AGGACATCAA GTGGGCCCTG CACGAGCTCA AAGGACACTA TGCAATCACC 
CGAAAGGCCT TTTCTGACGC CATTAAAAAA TGGCAGGAGC TATCGCCAGA AACCAGTGGA 
AAAAGAAAAA AGAGAAAAGA AATGAATCAG TATTCTTACA TAGATTTCAA ATTTGAACAA 
GGTGACAAAA AAATAGAGAA GAGGATGTTC TTTCTGGAAA ATAAGCGGCG ACATTGTAGG 
TCCTATGACC GGCGTGCCCT CCTTCCAGCT GTGCAGCAGG AGCAGGAGTT CTACGAGCAG 
AAAATCAAAG AGATGGCAGA GCATGAAGAC TTTTTGCTTG CCCTGCAGAT GAATGAAGAA 
CAGTATCAAA AGGATGGCCA GCTGATTGAG TGCCGCTGCT GCTATGGGGA GTTCCCATTC 
GAGGAGCTGA CGCAGTGTGC TGACGCTCAC TTATTCTGCA AAGAGTGTCT CATCAGATAC 
GCCCAAGAGG CAGTCTTTGG ATCTGGAAAG TCAGAGCTCA GCTGTATGGA GGGCAGCTGC 
ACATGCTCAT TCCCAGCCAG TGAACTGGAG AAGGTGCTTC CCCAGACCAT CCTGTATAAA 
TACTATGAGC GAAAAGCAGA GGAAGAAGTT GCAGCAGCCT ACGCTGATGA GCTTGTCAGG 
TGCCCCTCCT GCAGCTTTCC TGCTCTCCTG GACAGTGACG TGAAGAGGTT CAGTTGTCCC 
AATCCTCGCT GTCGAAAGGA AACCTGTAGG AAGTGTCAAG GACTCTGGAA AGAGCACAAC 
GGCCTCACTT GTGAGGAGCT GGCTGAAAAA GATGACATCA AGTACCGAAC ATCTATTGAA 
GAAAAAATGA CTGCTGCCCG CATTAGGAAA TGCCACAAGT GTGGGACCAG CCTCATCAAA 
TCTGAAGGCT GCAACCGCAT GTCCTGCCGC TGCGGTGCCC AGATGTGCTA CCTCTGCCGC 
GTGTCCATCA ATGGCTACGA CCACTTCTGC CAGCACCCTC GCTCCCCAGG AGCCCCCTGC 
CAGGAGTGTT CCCGATGCTC TCTCTGGACC GACCCCACTG AAGATGATGA GAAGCTAATT 
GAGGAAATCC AGAAGGAGGC CGAAGAGGAA CAGAAAAGAA AGAATGGAGA GAACACCTTC 
AAACGCATCG GCCCCCCCTT GGAGAAGCCC CCTGAGAAAG TGCAAAGGAC CGAGGCCCTG 
CCGAGGCCCG TGCCCCAGAA CCTGCACCAG CCGCAGATAC CCGCCTACGC CTTCGTGCAC 
CCACCCTTCC CCCTGCCGCC CGTCCGGCCC GTGTTCAACA ACTTCCCCCT CAACATGGGC 
CCCATCCCGG CACCCTACGT GCCGCCCCTG CCCAATATGC GCGTCAACTA CGAATTTGCC 
CCCATCCACC TGCCCCTGGA ACACAACCTG CCCATGCACT TTGGCCCCCA GCCTCGGCAT 
CGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_024984994.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840762.1 Bos taurus ring finger protein 216 (RNF216), transcript variant X2, mRNA.

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CloneID	OBa230338
Clone ID Related Accession (Same CDS sequence)	XM_024984994.1
Accession Version	XM_024984994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CTTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
CTGCTGTATC AGCATGAGCC CCGGGGGTCA GCTTTTCCAA GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAACAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGCAGCCCAA TTTGGGAAAC CTGTGGGGGC AAGAAGCTTC TTACGTAGAC 
CGGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA GGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGACGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGTATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ACGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840762.1
CDS	218..2389
Translation	MAEGNSSEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDFINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPLLYQHEPRGSAFPRPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETRQPNLGNLWGQEASYVDRELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSM

Target ORF information:

RefSeq Version	XM_024984994.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984994.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAG TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CTTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
CTGCTGTATC AGCATGAGCC CCGGGGGTCA GCTTTTCCAA GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAACAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGCAGCCCAA TTTGGGAAAC CTGTGGGGGC AAGAAGCTTC TTACGTAGAC 
CGGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA GGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGACGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGTATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ACGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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RNF216 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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