PIF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIF1
Entrez Gene ID	534330
Full Name	PIF1 5'-to-3' DNA helicase
General protein information	Preferred Names PIF1 5'-to-3' DNA helicase Names ATP-dependent DNA helicase PIF1 DNA helicase homolog PIF1 PIF1 5'-to-3' DNA helicase homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005211698.4	XP_005211755.2	ATP-dependent DNA helicase PIF1 isoform X1
NM_001206052.1	NP_001192981.1	ATP-dependent DNA helicase PIF1
XM_010809294.2	XP_010807596.1	ATP-dependent DNA helicase PIF1 isoform X2
XM_010809294.3	XP_010807596.1	ATP-dependent DNA helicase PIF1 isoform X2
XM_024997661.1	XP_024853429.1	ATP-dependent DNA helicase PIF1 isoform X3
XM_005211698.3	XP_005211755.2	ATP-dependent DNA helicase PIF1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pif1	NP_766041.1
Caenorhabditis elegans (roundworm)	pif-1	NP_490774.2
Xenopus tropicalis (tropical clawed frog)	pif1	NP_001016603.1
Homo sapiens (human)	PIF1	NP_001273426.1
	PIF1	XP_001108233.1
Gallus gallus (chicken)	PIF1	XP_426648.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007775	XP_317740.4
Xenopus tropicalis (tropical clawed frog)	LOC101730335	XP_004914011.1
Bos taurus (cattle)	PIF1	NP_001192981.1
Rattus norvegicus (Norway rat)	Pif1	NP_001037718.1
Danio rerio (zebrafish)	pif1	NP_942102.1
Pan troglodytes (chimpanzee)	PIF1	XP_510475.2
Canis lupus familiaris (dog)	PIF1	XP_003640106.1
Drosophila melanogaster (fruit fly)	CG3238	NP_608782.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PIF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219635	XM_024997661.1 Latest version!	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa01464	NM_001206052.1 Latest version!	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa57166	XM_005211698.4 Latest version!	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa57167	XM_010809294.3 Latest version!	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OBa57167	XM_010809294.2	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa57166	XM_005211698.3	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIF1 ( XM_024997661.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853429.1 Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa219635
Clone ID Related Accession (Same CDS sequence)	XM_024997661.1
Accession Version	XM_024997661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent DNA helicase PIF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCGG GCACCCAGGC AGCGGCCGCA GAATGCGAGG ACGGAGAACT GCGGTGCCGT 
GTGGCCGTGG AGGAGCTGAG CCCCGGTGGG CAGCCGCGAA GGCGCCAGTC CCTACGCACC 
GCGGAGCTAA GCCTGGGTCG TAACGAGCGC CGCGAGTTGA TGCTGAGGCT GCAGGCGCCC 
GGGCCCGCTG GGCGGCCACG CTGCTTCCCC GTGCGCGCCG CGCGCCTCTT CACCCGCTTC 
GCTGCAGCCG GGCGCAGCAC TCTGCGGTTC CCCGCCGACA GCACTCCCCG GGCCAGCGCC 
GTCCAGCTGC TGCTCTCCGA TTGCCCCCCG GACCGCCTGC GCCGCTTCCT GCACACGCTG 
CGCCTCAAGC TGGCGGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGCAC GCAGCTGCTT 
GGCCCGCGGC CCCGCGACTT CATCAGCATC AGCCCCGTGC AGCCCGAGGA GTTGCGGCGT 
GCGGCGGCCA CCCGGGTCAC GGATACCACG CCGGTGAAGC GGCCCACGGA ACCTCGGGCG 
GGAGCCAAGC CCCGCACCGA AGTCACAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGATTG 
CCCCCCACCA AACCAGAGCT TTCCAAGGAA CAGGCTGCTG TCCTGAGGGC TGTCCTGAAA 
GGCCAGAGCA TTTTCTTCAC TGGAAGTGCA GGGACGGGGA AGTCTTACCT GCTGAAGCGT 
ATCCTGGGCT CACTGCCTCC CACGGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGTACTAC CCTCCATGCC TTTGCAGGTA TTGGCTCAGG CCAGGCTCCC 
CTGGCCCAGT GTGTGGCCCT GGCCCAGCGG CCCGGTGTGC GGCAGGGTTG GCTGAACTGC 
CAGCGGCTAG TCATTGATGA GATCTCCATG GTGGAAGCGG ACCTGTTTGA CAAGCTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAT AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TCCTGCAGCT GCCACCTGTA ACCAAGGGAT CCCAGCTCCC GCAGTTCTGC 
TTCCAGGCCA AGAGCTGGAG GAGGTGTGTC CCAGTGACCC TGGAACTGAC TGAGGTGTGG 
AGGCAAACAG ACAAGACCTT CATCTCTCTG CTGCAAGCTG TGCGGCTGGG CAGGTGCTCA 
GATGAAGTCA CCCGCCAGCT CCGGGCCACA GCTGCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACAA GGCTCTGCAC CCACCAGGAT GACGTGGCCC TTACCAATGA GAGGCAGCTG 
CAGGAACTAC CAGGCGAAGT ACACAGCTTT GAGGCCATGG ACAGTGACCC TGAGCAAGCC 
CGGACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AGCTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAGAACTT GGCAGTGTCT CGGGGCTTGG TGAATGGTGC CCGAGGGGTG 
GTAGTCGGGT TCGAGGCCGA GGGGAGAGGG CTGCCCCAGG TACGGTTCCT GTGTGGAGTC 
ACCGAGGTCA TCCGTGCTGA CCGCTGGACG GTGCAGACCA CTGGAGGCCA GCTCCTCAGC 
CGGCAGCAGC TGCCCTTGCA GCTGGCCTGG GCCATTTCCA TTCACAAGAG CCAGGGCATG 
TCCCTGGATT GTGTGGAGAT GTCTCTGGGC CGTGTGTTTG CCAGCGGCCA GGCCTACGTG 
GCCCTTTCCC GGGCCCGCAG CCTGCAGGGC CTCCGTGTGT TGGACTTTGA CCCCATGGTG 
GTTCGCTGTG ACCCCCGCGT GCTGAGCTTC TATGCTACCC TGTGGCGGGA CAGGGCCCTC 
AGCCTGGTAA GAGGGAGTCA CCCAATGGTG GGCAGAATGT GGCCGTGGGT AGCCTGGGAG 
CTAAGAGGAA GGGAAGGCCA AAAACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853429.1
CDS	329..2278
Translation	MLSGTQAAAAECEDGELRCRVAVEELSPGGQPRRRQSLRTAELSLGRNERRELMLRLQAPGPAGRPRCFPVRAARLFTRFAAAGRSTLRFPADSTPRASAVQLLLSDCPPDRLRRFLHTLRLKLAAAPGPGPASARTQLLGPRPRDFISISPVQPEELRRAAATRVTDTTPVKRPTEPRAGAKPRTEVTRWPLPVKRLRLPPTKPELSKEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQLPQFCFQAKSWRRCVPVTLELTEVWRQTDKTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNERQLQELPGEVHSFEAMDSDPEQARTLDAQCPVSQLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIRADRWTVQTTGGQLLSRQQLPLQLAWAISIHKSQGMSLDCVEMSLGRVFASGQAYVALSRARSLQGLRVLDFDPMVVRCDPRVLSFYATLWRDRALSLVRGSHPMVGRMWPWVAWELRGREGQKH

Target ORF information:

RefSeq Version	XM_024997661.1
Organism	Bos taurus(cattle)
Definition	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997661.1

ORF Insert Sequence:

ATGCTTTCGG GCACCCAGGC AGCGGCCGCA GAATGCGAGG ACGGAGAACT GCGGTGCCGT 
GTGGCCGTGG AGGAGCTGAG CCCCGGTGGG CAGCCGCGAA GGCGCCAGTC CCTACGCACC 
GCGGAGCTAA GCCTGGGTCG TAACGAGCGC CGCGAGTTGA TGCTGAGGCT GCAGGCGCCC 
GGGCCCGCTG GGCGGCCACG CTGCTTCCCC GTGCGCGCCG CGCGCCTCTT CACCCGCTTC 
GCTGCAGCCG GGCGCAGCAC TCTGCGGTTC CCCGCCGACA GCACTCCCCG GGCCAGCGCC 
GTCCAGCTGC TGCTCTCCGA TTGCCCCCCG GACCGCCTGC GCCGCTTCCT GCACACGCTG 
CGCCTCAAGC TGGCGGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGCAC GCAGCTGCTT 
GGCCCGCGGC CCCGCGACTT CATCAGCATC AGCCCCGTGC AGCCCGAGGA GTTGCGGCGT 
GCGGCGGCCA CCCGGGTCAC GGATACCACG CCGGTGAAGC GGCCCACGGA ACCTCGGGCG 
GGAGCCAAGC CCCGCACCGA AGTCACAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGATTG 
CCCCCCACCA AACCAGAGCT TTCCAAGGAA CAGGCTGCTG TCCTGAGGGC TGTCCTGAAA 
GGCCAGAGCA TTTTCTTCAC TGGAAGTGCA GGGACGGGGA AGTCTTACCT GCTGAAGCGT 
ATCCTGGGCT CACTGCCTCC CACGGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGTACTAC CCTCCATGCC TTTGCAGGTA TTGGCTCAGG CCAGGCTCCC 
CTGGCCCAGT GTGTGGCCCT GGCCCAGCGG CCCGGTGTGC GGCAGGGTTG GCTGAACTGC 
CAGCGGCTAG TCATTGATGA GATCTCCATG GTGGAAGCGG ACCTGTTTGA CAAGCTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAT AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TCCTGCAGCT GCCACCTGTA ACCAAGGGAT CCCAGCTCCC GCAGTTCTGC 
TTCCAGGCCA AGAGCTGGAG GAGGTGTGTC CCAGTGACCC TGGAACTGAC TGAGGTGTGG 
AGGCAAACAG ACAAGACCTT CATCTCTCTG CTGCAAGCTG TGCGGCTGGG CAGGTGCTCA 
GATGAAGTCA CCCGCCAGCT CCGGGCCACA GCTGCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACAA GGCTCTGCAC CCACCAGGAT GACGTGGCCC TTACCAATGA GAGGCAGCTG 
CAGGAACTAC CAGGCGAAGT ACACAGCTTT GAGGCCATGG ACAGTGACCC TGAGCAAGCC 
CGGACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AGCTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAGAACTT GGCAGTGTCT CGGGGCTTGG TGAATGGTGC CCGAGGGGTG 
GTAGTCGGGT TCGAGGCCGA GGGGAGAGGG CTGCCCCAGG TACGGTTCCT GTGTGGAGTC 
ACCGAGGTCA TCCGTGCTGA CCGCTGGACG GTGCAGACCA CTGGAGGCCA GCTCCTCAGC 
CGGCAGCAGC TGCCCTTGCA GCTGGCCTGG GCCATTTCCA TTCACAAGAG CCAGGGCATG 
TCCCTGGATT GTGTGGAGAT GTCTCTGGGC CGTGTGTTTG CCAGCGGCCA GGCCTACGTG 
GCCCTTTCCC GGGCCCGCAG CCTGCAGGGC CTCCGTGTGT TGGACTTTGA CCCCATGGTG 
GTTCGCTGTG ACCCCCGCGT GCTGAGCTTC TATGCTACCC TGTGGCGGGA CAGGGCCCTC 
AGCCTGGTAA GAGGGAGTCA CCCAATGGTG GGCAGAATGT GGCCGTGGGT AGCCTGGGAG 
CTAAGAGGAA GGGAAGGCCA AAAACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_001206052.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192981.1 Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), mRNA.

Price & Availability↑

CloneID	OBa01464
Clone ID Related Accession (Same CDS sequence)	NM_001206052.1
Accession Version	NM_001206052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	ATP-dependent DNA helicase PIF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000010.1. On Apr 20, 2011 this sequence version replaced XM_614064.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145433, SAMN03145442 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTTTCGG GCACCCAGGC AGCGGCCGCA GAATGCGAGG ACGGAGAACT GCGGTGCCGT 
GTGGCCGTGG AGGAGCTGAG CCCCGGTGGG CAGCCGCGAA GGCGCCAGTC CCTACGCACC 
GCGGAGCTAA GCCTGGGTCG TAACGAGCGC CGCGAGTTGA TGCTGAGGCT GCAGGCGCCC 
GGGCCCGCTG GGCGGCCACG CTGCTTCCCC GTGCGCGCCG CGCGCCTCTT CACCCGCTTC 
GCTGCAGCCG GGCGCAGCAC TCTGCGGTTC CCCGCCGACA GCACTCCCCG GGCCAGCGCC 
GTCCAGCTGC TGCTCTCCGA TTGCCCCCCG GACCGCCTGC GCCGCTTCCT GCACACGCTG 
CGCCTCAAGC TGGCGGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGCAC GCAGCTGCTT 
GGCCCGCGGC CCCGCGACTT CATCAGCATC AGCCCCGTGC AGCCCGAGGA GTTGCGGCGT 
GCGGCGGCCA CCCGGGTCAC GGATACCACG CCGGTGAAGC GGCCCACGGA ACCTCGGGCG 
GGAGCCAAGC CCCGCACCGA AGTCACAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGATTG 
CCCCCCACCA AACCAGAGCT TTCCAAGGAA CAGGCTGCTG TCCTGAGGGC TGTCCTGAAA 
GGCCAGAGCA TTTTCTTCAC TGGAAGTGCA GGGACGGGGA AGTCTTACCT GCTGAAGCGT 
ATCCTGGGCT CACTGCCTCC CACGGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGTACTAC CCTCCATGCC TTTGCAGGTA TTGGCTCAGG CCAGGCTCCC 
CTGGCCCAGT GTGTGGCCCT GGCCCAGCGG CCCGGTGTGC GGCAGGGTTG GCTGAACTGC 
CAGCGGCTAG TCATTGATGA GATCTCCATG GTGGAAGCGG ACCTGTTTGA CAAGCTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAT AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TCCTGCAGCT GCCACCTGTA ACCAAGGGAT CCCAGCTCCC GCAGTTCTGC 
TTCCAGGCCA AGAGCTGGAG GAGGTGTGTC CCAGTGACCC TGGAACTGAC TGAGGTGTGG 
AGGCAAACAG ACAAGACCTT CATCTCTCTG CTGCAAGCTG TGCGGCTGGG CAGGTGCTCA 
GATGAAGTCA CCCGCCAGCT CCGGGCCACA GCTGCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACAA GGCTCTGCAC CCACCAGGAT GACGTGGCCC TTACCAATGA GAGGCAGCTG 
CAGGAACTAC CAGGCGAAGT ACACAGCTTT GAGGCCATGG ACAGTGACCC TGAGCAAGCC 
CGGACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AGCTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAGAACTT GGCAGTGTCT CGGGGCTTGG TGAATGGTGC CCGAGGGGTG 
GTAGTCGGGT TCGAGGCCGA GGGGAGAGGG CTGCCCCAGG TACGGTTCCT GTGTGGAGTC 
ACCGAGGTCA TCCGTGCTGA CCGCTGGACG GTGCAGACCA CTGGAGGCCA GCTCCTCAGC 
CGGCAGCAGC TGCCCTTGCA GCTGGCCTGG GCCATTTCCA TTCACAAGAG CCAGGGCATG 
TCCCTGGATT GTGTGGAGAT GTCTCTGGGC CGTGTGTTTG CCAGCGGCCA GGCCTACGTG 
GCCCTTTCCC GGGCCCGCAG CCTGCAGGGC CTCCGTGTGT TGGACTTTGA CCCCATGGTG 
GTTCGCTGTG ACCCCCGCGT GCTGAGCTTC TATGCTACCC TGTGGCGGGA CAGGGCCCTC 
AGCCTGGAGT CCCCAGATGA TGAGGAAGGA ACCTCAGACC AGGAGAACGT GGACCCGAAC 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192981.1
CDS	1..1926
Translation	MLSGTQAAAAECEDGELRCRVAVEELSPGGQPRRRQSLRTAELSLGRNERRELMLRLQAPGPAGRPRCFPVRAARLFTRFAAAGRSTLRFPADSTPRASAVQLLLSDCPPDRLRRFLHTLRLKLAAAPGPGPASARTQLLGPRPRDFISISPVQPEELRRAAATRVTDTTPVKRPTEPRAGAKPRTEVTRWPLPVKRLRLPPTKPELSKEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQLPQFCFQAKSWRRCVPVTLELTEVWRQTDKTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNERQLQELPGEVHSFEAMDSDPEQARTLDAQCPVSQLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIRADRWTVQTTGGQLLSRQQLPLQLAWAISIHKSQGMSLDCVEMSLGRVFASGQAYVALSRARSLQGLRVLDFDPMVVRCDPRVLSFYATLWRDRALSLESPDDEEGTSDQENVDPNL

Target ORF information:

RefSeq Version	NM_001206052.1
Organism	Bos taurus(cattle)
Definition	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206052.1

ORF Insert Sequence:

ATGCTTTCGG GCACCCAGGC AGCGGCCGCA GAATGCGAGG ACGGAGAACT GCGGTGCCGT 
GTGGCCGTGG AGGAGCTGAG CCCCGGTGGG CAGCCGCGAA GGCGCCAGTC CCTACGCACC 
GCGGAGCTAA GCCTGGGTCG TAACGAGCGC CGCGAGTTGA TGCTGAGGCT GCAGGCGCCC 
GGGCCCGCTG GGCGGCCACG CTGCTTCCCC GTGCGCGCCG CGCGCCTCTT CACCCGCTTC 
GCTGCAGCCG GGCGCAGCAC TCTGCGGTTC CCCGCCGACA GCACTCCCCG GGCCAGCGCC 
GTCCAGCTGC TGCTCTCCGA TTGCCCCCCG GACCGCCTGC GCCGCTTCCT GCACACGCTG 
CGCCTCAAGC TGGCGGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGCAC GCAGCTGCTT 
GGCCCGCGGC CCCGCGACTT CATCAGCATC AGCCCCGTGC AGCCCGAGGA GTTGCGGCGT 
GCGGCGGCCA CCCGGGTCAC GGATACCACG CCGGTGAAGC GGCCCACGGA ACCTCGGGCG 
GGAGCCAAGC CCCGCACCGA AGTCACAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGATTG 
CCCCCCACCA AACCAGAGCT TTCCAAGGAA CAGGCTGCTG TCCTGAGGGC TGTCCTGAAA 
GGCCAGAGCA TTTTCTTCAC TGGAAGTGCA GGGACGGGGA AGTCTTACCT GCTGAAGCGT 
ATCCTGGGCT CACTGCCTCC CACGGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGTACTAC CCTCCATGCC TTTGCAGGTA TTGGCTCAGG CCAGGCTCCC 
CTGGCCCAGT GTGTGGCCCT GGCCCAGCGG CCCGGTGTGC GGCAGGGTTG GCTGAACTGC 
CAGCGGCTAG TCATTGATGA GATCTCCATG GTGGAAGCGG ACCTGTTTGA CAAGCTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAT AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TCCTGCAGCT GCCACCTGTA ACCAAGGGAT CCCAGCTCCC GCAGTTCTGC 
TTCCAGGCCA AGAGCTGGAG GAGGTGTGTC CCAGTGACCC TGGAACTGAC TGAGGTGTGG 
AGGCAAACAG ACAAGACCTT CATCTCTCTG CTGCAAGCTG TGCGGCTGGG CAGGTGCTCA 
GATGAAGTCA CCCGCCAGCT CCGGGCCACA GCTGCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACAA GGCTCTGCAC CCACCAGGAT GACGTGGCCC TTACCAATGA GAGGCAGCTG 
CAGGAACTAC CAGGCGAAGT ACACAGCTTT GAGGCCATGG ACAGTGACCC TGAGCAAGCC 
CGGACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AGCTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAGAACTT GGCAGTGTCT CGGGGCTTGG TGAATGGTGC CCGAGGGGTG 
GTAGTCGGGT TCGAGGCCGA GGGGAGAGGG CTGCCCCAGG TACGGTTCCT GTGTGGAGTC 
ACCGAGGTCA TCCGTGCTGA CCGCTGGACG GTGCAGACCA CTGGAGGCCA GCTCCTCAGC 
CGGCAGCAGC TGCCCTTGCA GCTGGCCTGG GCCATTTCCA TTCACAAGAG CCAGGGCATG 
TCCCTGGATT GTGTGGAGAT GTCTCTGGGC CGTGTGTTTG CCAGCGGCCA GGCCTACGTG 
GCCCTTTCCC GGGCCCGCAG CCTGCAGGGC CTCCGTGTGT TGGACTTTGA CCCCATGGTG 
GTTCGCTGTG ACCCCCGCGT GCTGAGCTTC TATGCTACCC TGTGGCGGGA CAGGGCCCTC 
AGCCTGGAGT CCCCAGATGA TGAGGAAGGA ACCTCAGACC AGGAGAACGT GGACCCGAAC 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( XM_005211698.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211755.2 Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa57166
Clone ID Related Accession (Same CDS sequence)	XM_005211698.4 , XM_005211698.3
Accession Version	XM_005211698.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211698.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GTAAGAGGGA GTCACCCAAT GGTGGGCAGA ATGTGGCCGT GGGTAGCCTG GGAGCTAAGA 
GGAAGGGAAG GCCAAAAACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211755.2
CDS	135..2198
Translation	MILYSQNLKVSSVLLLPAGRLVSTGSSECWHEPAEAAAMLSGTQAAAAECEDGELRCRVAVEELSPGGQPRRRQSLRTAELSLGRNERRELMLRLQAPGPAGRPRCFPVRAARLFTRFAAAGRSTLRFPADSTPRASAVQLLLSDCPPDRLRRFLHTLRLKLAAAPGPGPASARTQLLGPRPRDFISISPVQPEELRRAAATRVTDTTPVKRPTEPRAGAKPRTEVTRWPLPVKRLRLPPTKPELSKEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQLPQFCFQAKSWRRCVPVTLELTEVWRQTDKTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNERQLQELPGEVHSFEAMDSDPEQARTLDAQCPVSQLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIRADRWTVQTTGGQLLSRQQLPLQLAWAISIHKSQGMSLDCVEMSLGRVFASGQAYVALSRARSLQGLRVLDFDPMVVRCDPRVLSFYATLWRDRALSLVRGSHPMVGRMWPWVAWELRGREGQKH

Target ORF information:

RefSeq Version	XM_005211698.4
Organism	Bos taurus(cattle)
Definition	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211698.4

ORF Insert Sequence:

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GTAAGAGGGA GTCACCCAAT GGTGGGCAGA ATGTGGCCGT GGGTAGCCTG GGAGCTAAGA 
GGAAGGGAAG GCCAAAAACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( XM_005211698.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211755.2 Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa57166
Clone ID Related Accession (Same CDS sequence)	XM_005211698.4 , XM_005211698.3
Accession Version	XM_005211698.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GTAAGAGGGA GTCACCCAAT GGTGGGCAGA ATGTGGCCGT GGGTAGCCTG GGAGCTAAGA 
GGAAGGGAAG GCCAAAAACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211755.2
CDS	1646..3709
Translation	MILYSQNLKVSSVLLLPAGRLVSTGSSECWHEPAEAAAMLSGTQAAAAECEDGELRCRVAVEELSPGGQPRRRQSLRTAELSLGRNERRELMLRLQAPGPAGRPRCFPVRAARLFTRFAAAGRSTLRFPADSTPRASAVQLLLSDCPPDRLRRFLHTLRLKLAAAPGPGPASARTQLLGPRPRDFISISPVQPEELRRAAATRVTDTTPVKRPTEPRAGAKPRTEVTRWPLPVKRLRLPPTKPELSKEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQLPQFCFQAKSWRRCVPVTLELTEVWRQTDKTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNERQLQELPGEVHSFEAMDSDPEQARTLDAQCPVSQLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIRADRWTVQTTGGQLLSRQQLPLQLAWAISIHKSQGMSLDCVEMSLGRVFASGQAYVALSRARSLQGLRVLDFDPMVVRCDPRVLSFYATLWRDRALSLVRGSHPMVGRMWPWVAWELRGREGQKH

Target ORF information:

RefSeq Version	XM_005211698.3
Organism	Bos taurus(cattle)
Definition	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211698.3

ORF Insert Sequence:

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GTAAGAGGGA GTCACCCAAT GGTGGGCAGA ATGTGGCCGT GGGTAGCCTG GGAGCTAAGA 
GGAAGGGAAG GCCAAAAACA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( XM_010809294.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807596.1 Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa57167
Clone ID Related Accession (Same CDS sequence)	XM_010809294.2 , XM_010809294.3
Accession Version	XM_010809294.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-dependent DNA helicase PIF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104280.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GAGTCCCCAG ATGATGAGGA AGGAACCTCA GACCAGGAGA ACGTGGACCC GAACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807596.1
CDS	1646..3685
Translation	MILYSQNLKVSSVLLLPAGRLVSTGSSECWHEPAEAAAMLSGTQAAAAECEDGELRCRVAVEELSPGGQPRRRQSLRTAELSLGRNERRELMLRLQAPGPAGRPRCFPVRAARLFTRFAAAGRSTLRFPADSTPRASAVQLLLSDCPPDRLRRFLHTLRLKLAAAPGPGPASARTQLLGPRPRDFISISPVQPEELRRAAATRVTDTTPVKRPTEPRAGAKPRTEVTRWPLPVKRLRLPPTKPELSKEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQLPQFCFQAKSWRRCVPVTLELTEVWRQTDKTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNERQLQELPGEVHSFEAMDSDPEQARTLDAQCPVSQLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIRADRWTVQTTGGQLLSRQQLPLQLAWAISIHKSQGMSLDCVEMSLGRVFASGQAYVALSRARSLQGLRVLDFDPMVVRCDPRVLSFYATLWRDRALSLESPDDEEGTSDQENVDPNL

Target ORF information:

RefSeq Version	XM_010809294.2
Organism	Bos taurus(cattle)
Definition	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809294.2

ORF Insert Sequence:

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GAGTCCCCAG ATGATGAGGA AGGAACCTCA GACCAGGAGA ACGTGGACCC GAACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( XM_010809294.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807596.1 Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.

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CloneID	OBa57167
Clone ID Related Accession (Same CDS sequence)	XM_010809294.2 , XM_010809294.3
Accession Version	XM_010809294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-dependent DNA helicase PIF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GAGTCCCCAG ATGATGAGGA AGGAACCTCA GACCAGGAGA ACGTGGACCC GAACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807596.1
CDS	134..2173
Translation	MILYSQNLKVSSVLLLPAGRLVSTGSSECWHEPAEAAAMLSGTQAAAAECEDGELRCRVAVEELSPGGQPRRRQSLRTAELSLGRNERRELMLRLQAPGPAGRPRCFPVRAARLFTRFAAAGRSTLRFPADSTPRASAVQLLLSDCPPDRLRRFLHTLRLKLAAAPGPGPASARTQLLGPRPRDFISISPVQPEELRRAAATRVTDTTPVKRPTEPRAGAKPRTEVTRWPLPVKRLRLPPTKPELSKEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQLPQFCFQAKSWRRCVPVTLELTEVWRQTDKTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNERQLQELPGEVHSFEAMDSDPEQARTLDAQCPVSQLLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIRADRWTVQTTGGQLLSRQQLPLQLAWAISIHKSQGMSLDCVEMSLGRVFASGQAYVALSRARSLQGLRVLDFDPMVVRCDPRVLSFYATLWRDRALSLESPDDEEGTSDQENVDPNL

Target ORF information:

RefSeq Version	XM_010809294.3
Organism	Bos taurus(cattle)
Definition	Bos taurus PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809294.3

ORF Insert Sequence:

ATGATTCTCT ACTCCCAGAA TTTGAAAGTT TCCAGTGTGC TACTACTGCC TGCAGGCCGA 
CTTGTCTCAA CTGGTTCGTC CGAGTGCTGG CACGAACCTG CAGAGGCGGC GGCGATGCTT 
TCGGGCACCC AGGCAGCGGC CGCAGAATGC GAGGACGGAG AACTGCGGTG CCGTGTGGCC 
GTGGAGGAGC TGAGCCCCGG TGGGCAGCCG CGAAGGCGCC AGTCCCTACG CACCGCGGAG 
CTAAGCCTGG GTCGTAACGA GCGCCGCGAG TTGATGCTGA GGCTGCAGGC GCCCGGGCCC 
GCTGGGCGGC CACGCTGCTT CCCCGTGCGC GCCGCGCGCC TCTTCACCCG CTTCGCTGCA 
GCCGGGCGCA GCACTCTGCG GTTCCCCGCC GACAGCACTC CCCGGGCCAG CGCCGTCCAG 
CTGCTGCTCT CCGATTGCCC CCCGGACCGC CTGCGCCGCT TCCTGCACAC GCTGCGCCTC 
AAGCTGGCGG CGGCCCCGGG TCCCGGGCCG GCCTCCGCCC GCACGCAGCT GCTTGGCCCG 
CGGCCCCGCG ACTTCATCAG CATCAGCCCC GTGCAGCCCG AGGAGTTGCG GCGTGCGGCG 
GCCACCCGGG TCACGGATAC CACGCCGGTG AAGCGGCCCA CGGAACCTCG GGCGGGAGCC 
AAGCCCCGCA CCGAAGTCAC AAGGTGGCCC CTGCCTGTGA AGAGGCTGAG ATTGCCCCCC 
ACCAAACCAG AGCTTTCCAA GGAACAGGCT GCTGTCCTGA GGGCTGTCCT GAAAGGCCAG 
AGCATTTTCT TCACTGGAAG TGCAGGGACG GGGAAGTCTT ACCTGCTGAA GCGTATCCTG 
GGCTCACTGC CTCCCACGGG CACTGTGGCC ACTGCCAGCA CTGGGGTGGC AGCCTGCCAC 
ATCGGGGGTA CTACCCTCCA TGCCTTTGCA GGTATTGGCT CAGGCCAGGC TCCCCTGGCC 
CAGTGTGTGG CCCTGGCCCA GCGGCCCGGT GTGCGGCAGG GTTGGCTGAA CTGCCAGCGG 
CTAGTCATTG ATGAGATCTC CATGGTGGAA GCGGACCTGT TTGACAAGCT GGAGGCCGTG 
GCCAGAGCTG TCCGGCAGCA GAATAAGCCA TTCGGAGGGA TCCAGCTCAT CATCTGTGGG 
GACTTCCTGC AGCTGCCACC TGTAACCAAG GGATCCCAGC TCCCGCAGTT CTGCTTCCAG 
GCCAAGAGCT GGAGGAGGTG TGTCCCAGTG ACCCTGGAAC TGACTGAGGT GTGGAGGCAA 
ACAGACAAGA CCTTCATCTC TCTGCTGCAA GCTGTGCGGC TGGGCAGGTG CTCAGATGAA 
GTCACCCGCC AGCTCCGGGC CACAGCTGCC CACAAGGTGG GGCGAGATGG GATTGTGGCC 
ACAAGGCTCT GCACCCACCA GGATGACGTG GCCCTTACCA ATGAGAGGCA GCTGCAGGAA 
CTACCAGGCG AAGTACACAG CTTTGAGGCC ATGGACAGTG ACCCTGAGCA AGCCCGGACC 
CTGGATGCCC AGTGTCCTGT TAGCCAGCTC CTTCAGCTAA AGCTGGGGGC CCAGGTGATG 
CTGGTGAAGA ACTTGGCAGT GTCTCGGGGC TTGGTGAATG GTGCCCGAGG GGTGGTAGTC 
GGGTTCGAGG CCGAGGGGAG AGGGCTGCCC CAGGTACGGT TCCTGTGTGG AGTCACCGAG 
GTCATCCGTG CTGACCGCTG GACGGTGCAG ACCACTGGAG GCCAGCTCCT CAGCCGGCAG 
CAGCTGCCCT TGCAGCTGGC CTGGGCCATT TCCATTCACA AGAGCCAGGG CATGTCCCTG 
GATTGTGTGG AGATGTCTCT GGGCCGTGTG TTTGCCAGCG GCCAGGCCTA CGTGGCCCTT 
TCCCGGGCCC GCAGCCTGCA GGGCCTCCGT GTGTTGGACT TTGACCCCAT GGTGGTTCGC 
TGTGACCCCC GCGTGCTGAG CTTCTATGCT ACCCTGTGGC GGGACAGGGC CCTCAGCCTG 
GAGTCCCCAG ATGATGAGGA AGGAACCTCA GACCAGGAGA ACGTGGACCC GAACCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PIF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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FAX

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