WAPL cDNA ORF clone, Bos taurus(cattle)

The following WAPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WAPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa12497 XM_005193121.3
Latest version!
Bos taurus WAPL cohesin release factor (WAPL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12497 XM_024986914.1
Latest version!
Bos taurus WAPL cohesin release factor (WAPL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12497 XM_002698897.6
Latest version!
Bos taurus WAPL cohesin release factor (WAPL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12497 XM_005226523.4
Latest version!
Bos taurus WAPL cohesin release factor (WAPL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12497 XM_001789058.6
Latest version!
Bos taurus WAPL cohesin release factor (WAPL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12498 XM_005226524.4
Latest version!
Bos taurus WAPL cohesin release factor (WAPL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12498 XM_005193122.3
Latest version!
Bos taurus WAPL cohesin release factor (WAPL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa12497 XM_002698897.5 Bos taurus WAPL cohesin release factor (WAPL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12498 XM_005226524.3 Bos taurus WAPL cohesin release factor (WAPL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12497 XM_005226523.3 Bos taurus WAPL cohesin release factor (WAPL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa12497
    Clone ID Related Accession (Same CDS sequence) XM_024986914.1 , XM_005226523.4 , XM_002698897.5 , XM_001789058.6 , XM_002698897.6 , XM_005193121.3 , XM_005226523.3
    Accession Version XM_024986914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842682.1
    CDS42..3623
    Translation

    Target ORF information:

    RefSeq Version XM_024986914.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12497
    Clone ID Related Accession (Same CDS sequence) XM_024986914.1 , XM_005226523.4 , XM_002698897.5 , XM_001789058.6 , XM_002698897.6 , XM_005193121.3 , XM_005226523.3
    Accession Version XM_005226523.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226523.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226580.1
    CDS267..3848
    Translation

    Target ORF information:

    RefSeq Version XM_005226523.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226523.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12497
    Clone ID Related Accession (Same CDS sequence) XM_024986914.1 , XM_005226523.4 , XM_002698897.5 , XM_001789058.6 , XM_002698897.6 , XM_005193121.3 , XM_005226523.3
    Accession Version XM_002698897.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002698897.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002698943.2
    CDS499..4080
    Translation

    Target ORF information:

    RefSeq Version XM_002698897.5
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002698897.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12497
    Clone ID Related Accession (Same CDS sequence) XM_024986914.1 , XM_005226523.4 , XM_002698897.5 , XM_001789058.6 , XM_002698897.6 , XM_005193121.3 , XM_005226523.3
    Accession Version XM_001789058.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001789058.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001789110.3
    CDS499..4080
    Translation

    Target ORF information:

    RefSeq Version XM_001789058.6
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001789058.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12497
    Clone ID Related Accession (Same CDS sequence) XM_024986914.1 , XM_005226523.4 , XM_002698897.5 , XM_001789058.6 , XM_002698897.6 , XM_005193121.3 , XM_005226523.3
    Accession Version XM_002698897.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002698897.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002698943.2
    CDS489..4070
    Translation

    Target ORF information:

    RefSeq Version XM_002698897.6
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002698897.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12497
    Clone ID Related Accession (Same CDS sequence) XM_024986914.1 , XM_005226523.4 , XM_002698897.5 , XM_001789058.6 , XM_002698897.6 , XM_005193121.3 , XM_005226523.3
    Accession Version XM_005193121.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193178.1
    CDS189..3770
    Translation

    Target ORF information:

    RefSeq Version XM_005193121.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193121.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12497
    Clone ID Related Accession (Same CDS sequence) XM_024986914.1 , XM_005226523.4 , XM_002698897.5 , XM_001789058.6 , XM_002698897.6 , XM_005193121.3 , XM_005226523.3
    Accession Version XM_005226523.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226580.1
    CDS189..3770
    Translation

    Target ORF information:

    RefSeq Version XM_005226523.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226523.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAAAACT TGGATTTTAC AGAGGACTTG
    CCTGGTGTGC CTGAAAGTGT GAAGAAGCCT CCAAATAAAC AAGGAGATAA ATCAAAGGAA
    AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCTCCTA CAAAAGCCGT ATATAATGCT
    AGACATTGGA ATCATCCAGA CTCGGAAGAA CTACCTGCAC CACCAGTAGT AAAACCTCAG
    AGTGTCACAG TGAGGCTGTC TTCAAAGGAG CAAAATCAAA AAGATGATGG AGTTTTTAAG
    GCTCCTGCAC CACCACCCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA
    GATATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTGTATAC TGTTGTTCAG
    CACGTGAAGC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ACCAAGAGTT CACTGATGAC
    ATTGAGTACT TGTTAAGTGG CTTAAAGAGC ACACAGCCTC TAAACACACG TTGCCTTAGT
    GTTATTAGCT TGGCTACTAA ATGTGCCATG CCCAGTTTTC GAATGCACCT GAGAGCACAT
    GGAATGGTAG CAATGGTCTT TAAAACCTTG GATGATTCCC AGCATCATCA GAATCTGTCC
    CTTTGTACAG CTGCCCTCAT GTACATTCTG AGTAGAGATC GTTTAAACAT GGATCTTGAT
    AGAGCTAGCC TAGATCTGAT GATTCGACTC CTAGAACTGG AACAAGATGC TTCTTCAGCT
    AAGCTGCTGA ATGAAAAAGA CATGAACAAA ATCAAAGAAA AAATTCGAAG GCTCTGTGAA
    ACTGTACACA ATAAGCACCT TGATCTAGAA AATATAACGA CTGGGCATTT AGCTATGGAG
    ACTTTACTGT CCCTTACTTC TAAACGAGCA GGAGACTGGT TTAAAGAAGA ACTACGGCTT
    TTGGGTGGTC TGGATCATAT TGTAGATAAA GTGAAAGAAT GTGTGGATCA TTTAAGTAAA
    GATGAGGATG AAGAGAAATT AGTAGCCTCA TTATGGGGAG CAGAGAGATG TTTACGAGTT
    TTAGAAAGCG TAACAGTGCA TAATCCAGAG AATCAAAGCT ACTTGATTGC CTATAAAGAT
    TCACAACTCA TTGTTTCGTC AGCTAAGGCA TTACAACATT GTGAAGAGCT GATTAAGCAG
    TATAACCGTG CTGAGAACAG TATATGCTTA CCTGACAGTA AGCCTCTGCC TCAGCAGAAT
    GTAACTAACC ACGTGGGCAA AGCAGTGGAG GACTGCATGA GGGCCATCAT TGGGGTGCTG
    CTCAACTTAA CTAATGATAA TGAATGGGGC AGCACCAAAA CAGGAGAGCA GGATGGCTTG
    ATTGGCACAG CCATGAATTG TGTGCTTCAG GTTCCAAAGT ACCTACCTCA GGAGCAGAGA
    TTTGATATTC GAGTGCTGGG TTTAGGTCTA CTGATAAATC TAGTGGAATA TAGTGCTCGA
    AATCGGCACT GTCTTGTCAA CATGGAAACA TCATGTTCTT TTGATTCTTC CTTCTGTAGT
    GGAGAAGGAG ATGATAGTTT AAGGATAGCT GGACAAGTTC ATGCAGTTCA GGCTTTAGTG
    CAGCTGTTCC TTGAGCGAGA GCGAGCAGCA CAGTTGGCAG AAAGTAAAAC AGATGAACTG
    ATCAAAGATG CTCCCACCAC TCAGCATGAC AAGAGTGGGG AGTGGCAGGA GACGAGTGGG
    GAGATACAGT GGGTGTCAAC TGAGAAGGCT GATGGTGCAG AAGAGAAAAA TAAGAAGGAG
    GAGGACGATG AAGAACTTGA CCTCAATAAA GCCCTTCAGC ATGCTGGCAA GCACATGGAG
    GATTGCATTG TGGCCTCATA CACAGCACTG CTTCTTGGGT GTCTCTGCCA GGAGAGTCCA
    ATCAATGTGA CCACTGTGCG GGAGTATCTG CCAGAAGGGG ACTTTTCAAT AATGACAGAG
    ATGCTCAAAA AATTCCTGAG CTTCATGAAT CTTACCTGTG CTGTTGGAAC CACGGGCCAG
    AAGTCCATCT CTAGAGTGAT TGAGTACTTG GAACACTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12498
    Clone ID Related Accession (Same CDS sequence) XM_005226524.3 , XM_005193122.3 , XM_005226524.4
    Accession Version XM_005226524.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAGGACT TGCCTGGTGT GCCTGAAAGT
    GTGAAGAAGC CTCCAAATAA ACAAGGAGAT AAATCAAAGG AAAATACCAG AAAGATTTTT
    AGTGGCCCCA AACGGTCTCC TACAAAAGCC GTATATAATG CTAGACATTG GAATCATCCA
    GACTCGGAAG AACTACCTGC ACCACCAGTA GTAAAACCTC AGAGTGTCAC AGTGAGGCTG
    TCTTCAAAGG AGCAAAATCA AAAAGATGAT GGAGTTTTTA AGGCTCCTGC ACCACCACCC
    AAAGTGATAA AAACTGTGAC AATACCTACT CAGCCCTACC AAGATATAGT TACTGCACTG
    AAATGCAGAA AAGAAGACAA AGAATTGTAT ACTGTTGTTC AGCACGTGAA GCACTTCAAT
    GATGTGGTGG AATTTGGTGA AAACCAAGAG TTCACTGATG ACATTGAGTA CTTGTTAAGT
    GGCTTAAAGA GCACACAGCC TCTAAACACA CGTTGCCTTA GTGTTATTAG CTTGGCTACT
    AAATGTGCCA TGCCCAGTTT TCGAATGCAC CTGAGAGCAC ATGGAATGGT AGCAATGGTC
    TTTAAAACCT TGGATGATTC CCAGCATCAT CAGAATCTGT CCCTTTGTAC AGCTGCCCTC
    ATGTACATTC TGAGTAGAGA TCGTTTAAAC ATGGATCTTG ATAGAGCTAG CCTAGATCTG
    ATGATTCGAC TCCTAGAACT GGAACAAGAT GCTTCTTCAG CTAAGCTGCT GAATGAAAAA
    GACATGAACA AAATCAAAGA AAAAATTCGA AGGCTCTGTG AAACTGTACA CAATAAGCAC
    CTTGATCTAG AAAATATAAC GACTGGGCAT TTAGCTATGG AGACTTTACT GTCCCTTACT
    TCTAAACGAG CAGGAGACTG GTTTAAAGAA GAACTACGGC TTTTGGGTGG TCTGGATCAT
    ATTGTAGATA AAGTGAAAGA ATGTGTGGAT CATTTAAGTA AAGATGAGGA TGAAGAGAAA
    TTAGTAGCCT CATTATGGGG AGCAGAGAGA TGTTTACGAG TTTTAGAAAG CGTAACAGTG
    CATAATCCAG AGAATCAAAG CTACTTGATT GCCTATAAAG ATTCACAACT CATTGTTTCG
    TCAGCTAAGG CATTACAACA TTGTGAAGAG CTGATTAAGC AGTATAACCG TGCTGAGAAC
    AGTATATGCT TACCTGACAG TAAGCCTCTG CCTCAGCAGA ATGTAACTAA CCACGTGGGC
    AAAGCAGTGG AGGACTGCAT GAGGGCCATC ATTGGGGTGC TGCTCAACTT AACTAATGAT
    AATGAATGGG GCAGCACCAA AACAGGAGAG CAGGATGGCT TGATTGGCAC AGCCATGAAT
    TGTGTGCTTC AGGTTCCAAA GTACCTACCT CAGGAGCAGA GATTTGATAT TCGAGTGCTG
    GGTTTAGGTC TACTGATAAA TCTAGTGGAA TATAGTGCTC GAAATCGGCA CTGTCTTGTC
    AACATGGAAA CATCATGTTC TTTTGATTCT TCCTTCTGTA GTGGAGAAGG AGATGATAGT
    TTAAGGATAG CTGGACAAGT TCATGCAGTT CAGGCTTTAG TGCAGCTGTT CCTTGAGCGA
    GAGCGAGCAG CACAGTTGGC AGAAAGTAAA ACAGATGAAC TGATCAAAGA TGCTCCCACC
    ACTCAGCATG ACAAGAGTGG GGAGTGGCAG GAGACGAGTG GGGAGATACA GTGGGTGTCA
    ACTGAGAAGG CTGATGGTGC AGAAGAGAAA AATAAGAAGG AGGAGGACGA TGAAGAACTT
    GACCTCAATA AAGCCCTTCA GCATGCTGGC AAGCACATGG AGGATTGCAT TGTGGCCTCA
    TACACAGCAC TGCTTCTTGG GTGTCTCTGC CAGGAGAGTC CAATCAATGT GACCACTGTG
    CGGGAGTATC TGCCAGAAGG GGACTTTTCA ATAATGACAG AGATGCTCAA AAAATTCCTG
    AGCTTCATGA ATCTTACCTG TGCTGTTGGA ACCACGGGCC AGAAGTCCAT CTCTAGAGTG
    ATTGAGTACT TGGAACACTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226581.1
    CDS499..4062
    Translation

    Target ORF information:

    RefSeq Version XM_005226524.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226524.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAGGACT TGCCTGGTGT GCCTGAAAGT
    GTGAAGAAGC CTCCAAATAA ACAAGGAGAT AAATCAAAGG AAAATACCAG AAAGATTTTT
    AGTGGCCCCA AACGGTCTCC TACAAAAGCC GTATATAATG CTAGACATTG GAATCATCCA
    GACTCGGAAG AACTACCTGC ACCACCAGTA GTAAAACCTC AGAGTGTCAC AGTGAGGCTG
    TCTTCAAAGG AGCAAAATCA AAAAGATGAT GGAGTTTTTA AGGCTCCTGC ACCACCACCC
    AAAGTGATAA AAACTGTGAC AATACCTACT CAGCCCTACC AAGATATAGT TACTGCACTG
    AAATGCAGAA AAGAAGACAA AGAATTGTAT ACTGTTGTTC AGCACGTGAA GCACTTCAAT
    GATGTGGTGG AATTTGGTGA AAACCAAGAG TTCACTGATG ACATTGAGTA CTTGTTAAGT
    GGCTTAAAGA GCACACAGCC TCTAAACACA CGTTGCCTTA GTGTTATTAG CTTGGCTACT
    AAATGTGCCA TGCCCAGTTT TCGAATGCAC CTGAGAGCAC ATGGAATGGT AGCAATGGTC
    TTTAAAACCT TGGATGATTC CCAGCATCAT CAGAATCTGT CCCTTTGTAC AGCTGCCCTC
    ATGTACATTC TGAGTAGAGA TCGTTTAAAC ATGGATCTTG ATAGAGCTAG CCTAGATCTG
    ATGATTCGAC TCCTAGAACT GGAACAAGAT GCTTCTTCAG CTAAGCTGCT GAATGAAAAA
    GACATGAACA AAATCAAAGA AAAAATTCGA AGGCTCTGTG AAACTGTACA CAATAAGCAC
    CTTGATCTAG AAAATATAAC GACTGGGCAT TTAGCTATGG AGACTTTACT GTCCCTTACT
    TCTAAACGAG CAGGAGACTG GTTTAAAGAA GAACTACGGC TTTTGGGTGG TCTGGATCAT
    ATTGTAGATA AAGTGAAAGA ATGTGTGGAT CATTTAAGTA AAGATGAGGA TGAAGAGAAA
    TTAGTAGCCT CATTATGGGG AGCAGAGAGA TGTTTACGAG TTTTAGAAAG CGTAACAGTG
    CATAATCCAG AGAATCAAAG CTACTTGATT GCCTATAAAG ATTCACAACT CATTGTTTCG
    TCAGCTAAGG CATTACAACA TTGTGAAGAG CTGATTAAGC AGTATAACCG TGCTGAGAAC
    AGTATATGCT TACCTGACAG TAAGCCTCTG CCTCAGCAGA ATGTAACTAA CCACGTGGGC
    AAAGCAGTGG AGGACTGCAT GAGGGCCATC ATTGGGGTGC TGCTCAACTT AACTAATGAT
    AATGAATGGG GCAGCACCAA AACAGGAGAG CAGGATGGCT TGATTGGCAC AGCCATGAAT
    TGTGTGCTTC AGGTTCCAAA GTACCTACCT CAGGAGCAGA GATTTGATAT TCGAGTGCTG
    GGTTTAGGTC TACTGATAAA TCTAGTGGAA TATAGTGCTC GAAATCGGCA CTGTCTTGTC
    AACATGGAAA CATCATGTTC TTTTGATTCT TCCTTCTGTA GTGGAGAAGG AGATGATAGT
    TTAAGGATAG CTGGACAAGT TCATGCAGTT CAGGCTTTAG TGCAGCTGTT CCTTGAGCGA
    GAGCGAGCAG CACAGTTGGC AGAAAGTAAA ACAGATGAAC TGATCAAAGA TGCTCCCACC
    ACTCAGCATG ACAAGAGTGG GGAGTGGCAG GAGACGAGTG GGGAGATACA GTGGGTGTCA
    ACTGAGAAGG CTGATGGTGC AGAAGAGAAA AATAAGAAGG AGGAGGACGA TGAAGAACTT
    GACCTCAATA AAGCCCTTCA GCATGCTGGC AAGCACATGG AGGATTGCAT TGTGGCCTCA
    TACACAGCAC TGCTTCTTGG GTGTCTCTGC CAGGAGAGTC CAATCAATGT GACCACTGTG
    CGGGAGTATC TGCCAGAAGG GGACTTTTCA ATAATGACAG AGATGCTCAA AAAATTCCTG
    AGCTTCATGA ATCTTACCTG TGCTGTTGGA ACCACGGGCC AGAAGTCCAT CTCTAGAGTG
    ATTGAGTACT TGGAACACTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12498
    Clone ID Related Accession (Same CDS sequence) XM_005226524.3 , XM_005193122.3 , XM_005226524.4
    Accession Version XM_005193122.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646758.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193122.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAGGACT TGCCTGGTGT GCCTGAAAGT
    GTGAAGAAGC CTCCAAATAA ACAAGGAGAT AAATCAAAGG AAAATACCAG AAAGATTTTT
    AGTGGCCCCA AACGGTCTCC TACAAAAGCC GTATATAATG CTAGACATTG GAATCATCCA
    GACTCGGAAG AACTACCTGC ACCACCAGTA GTAAAACCTC AGAGTGTCAC AGTGAGGCTG
    TCTTCAAAGG AGCAAAATCA AAAAGATGAT GGAGTTTTTA AGGCTCCTGC ACCACCACCC
    AAAGTGATAA AAACTGTGAC AATACCTACT CAGCCCTACC AAGATATAGT TACTGCACTG
    AAATGCAGAA AAGAAGACAA AGAATTGTAT ACTGTTGTTC AGCACGTGAA GCACTTCAAT
    GATGTGGTGG AATTTGGTGA AAACCAAGAG TTCACTGATG ACATTGAGTA CTTGTTAAGT
    GGCTTAAAGA GCACACAGCC TCTAAACACA CGTTGCCTTA GTGTTATTAG CTTGGCTACT
    AAATGTGCCA TGCCCAGTTT TCGAATGCAC CTGAGAGCAC ATGGAATGGT AGCAATGGTC
    TTTAAAACCT TGGATGATTC CCAGCATCAT CAGAATCTGT CCCTTTGTAC AGCTGCCCTC
    ATGTACATTC TGAGTAGAGA TCGTTTAAAC ATGGATCTTG ATAGAGCTAG CCTAGATCTG
    ATGATTCGAC TCCTAGAACT GGAACAAGAT GCTTCTTCAG CTAAGCTGCT GAATGAAAAA
    GACATGAACA AAATCAAAGA AAAAATTCGA AGGCTCTGTG AAACTGTACA CAATAAGCAC
    CTTGATCTAG AAAATATAAC GACTGGGCAT TTAGCTATGG AGACTTTACT GTCCCTTACT
    TCTAAACGAG CAGGAGACTG GTTTAAAGAA GAACTACGGC TTTTGGGTGG TCTGGATCAT
    ATTGTAGATA AAGTGAAAGA ATGTGTGGAT CATTTAAGTA AAGATGAGGA TGAAGAGAAA
    TTAGTAGCCT CATTATGGGG AGCAGAGAGA TGTTTACGAG TTTTAGAAAG CGTAACAGTG
    CATAATCCAG AGAATCAAAG CTACTTGATT GCCTATAAAG ATTCACAACT CATTGTTTCG
    TCAGCTAAGG CATTACAACA TTGTGAAGAG CTGATTAAGC AGTATAACCG TGCTGAGAAC
    AGTATATGCT TACCTGACAG TAAGCCTCTG CCTCAGCAGA ATGTAACTAA CCACGTGGGC
    AAAGCAGTGG AGGACTGCAT GAGGGCCATC ATTGGGGTGC TGCTCAACTT AACTAATGAT
    AATGAATGGG GCAGCACCAA AACAGGAGAG CAGGATGGCT TGATTGGCAC AGCCATGAAT
    TGTGTGCTTC AGGTTCCAAA GTACCTACCT CAGGAGCAGA GATTTGATAT TCGAGTGCTG
    GGTTTAGGTC TACTGATAAA TCTAGTGGAA TATAGTGCTC GAAATCGGCA CTGTCTTGTC
    AACATGGAAA CATCATGTTC TTTTGATTCT TCCTTCTGTA GTGGAGAAGG AGATGATAGT
    TTAAGGATAG CTGGACAAGT TCATGCAGTT CAGGCTTTAG TGCAGCTGTT CCTTGAGCGA
    GAGCGAGCAG CACAGTTGGC AGAAAGTAAA ACAGATGAAC TGATCAAAGA TGCTCCCACC
    ACTCAGCATG ACAAGAGTGG GGAGTGGCAG GAGACGAGTG GGGAGATACA GTGGGTGTCA
    ACTGAGAAGG CTGATGGTGC AGAAGAGAAA AATAAGAAGG AGGAGGACGA TGAAGAACTT
    GACCTCAATA AAGCCCTTCA GCATGCTGGC AAGCACATGG AGGATTGCAT TGTGGCCTCA
    TACACAGCAC TGCTTCTTGG GTGTCTCTGC CAGGAGAGTC CAATCAATGT GACCACTGTG
    CGGGAGTATC TGCCAGAAGG GGACTTTTCA ATAATGACAG AGATGCTCAA AAAATTCCTG
    AGCTTCATGA ATCTTACCTG TGCTGTTGGA ACCACGGGCC AGAAGTCCAT CTCTAGAGTG
    ATTGAGTACT TGGAACACTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193179.1
    CDS499..4062
    Translation

    Target ORF information:

    RefSeq Version XM_005193122.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193122.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAGGACT TGCCTGGTGT GCCTGAAAGT
    GTGAAGAAGC CTCCAAATAA ACAAGGAGAT AAATCAAAGG AAAATACCAG AAAGATTTTT
    AGTGGCCCCA AACGGTCTCC TACAAAAGCC GTATATAATG CTAGACATTG GAATCATCCA
    GACTCGGAAG AACTACCTGC ACCACCAGTA GTAAAACCTC AGAGTGTCAC AGTGAGGCTG
    TCTTCAAAGG AGCAAAATCA AAAAGATGAT GGAGTTTTTA AGGCTCCTGC ACCACCACCC
    AAAGTGATAA AAACTGTGAC AATACCTACT CAGCCCTACC AAGATATAGT TACTGCACTG
    AAATGCAGAA AAGAAGACAA AGAATTGTAT ACTGTTGTTC AGCACGTGAA GCACTTCAAT
    GATGTGGTGG AATTTGGTGA AAACCAAGAG TTCACTGATG ACATTGAGTA CTTGTTAAGT
    GGCTTAAAGA GCACACAGCC TCTAAACACA CGTTGCCTTA GTGTTATTAG CTTGGCTACT
    AAATGTGCCA TGCCCAGTTT TCGAATGCAC CTGAGAGCAC ATGGAATGGT AGCAATGGTC
    TTTAAAACCT TGGATGATTC CCAGCATCAT CAGAATCTGT CCCTTTGTAC AGCTGCCCTC
    ATGTACATTC TGAGTAGAGA TCGTTTAAAC ATGGATCTTG ATAGAGCTAG CCTAGATCTG
    ATGATTCGAC TCCTAGAACT GGAACAAGAT GCTTCTTCAG CTAAGCTGCT GAATGAAAAA
    GACATGAACA AAATCAAAGA AAAAATTCGA AGGCTCTGTG AAACTGTACA CAATAAGCAC
    CTTGATCTAG AAAATATAAC GACTGGGCAT TTAGCTATGG AGACTTTACT GTCCCTTACT
    TCTAAACGAG CAGGAGACTG GTTTAAAGAA GAACTACGGC TTTTGGGTGG TCTGGATCAT
    ATTGTAGATA AAGTGAAAGA ATGTGTGGAT CATTTAAGTA AAGATGAGGA TGAAGAGAAA
    TTAGTAGCCT CATTATGGGG AGCAGAGAGA TGTTTACGAG TTTTAGAAAG CGTAACAGTG
    CATAATCCAG AGAATCAAAG CTACTTGATT GCCTATAAAG ATTCACAACT CATTGTTTCG
    TCAGCTAAGG CATTACAACA TTGTGAAGAG CTGATTAAGC AGTATAACCG TGCTGAGAAC
    AGTATATGCT TACCTGACAG TAAGCCTCTG CCTCAGCAGA ATGTAACTAA CCACGTGGGC
    AAAGCAGTGG AGGACTGCAT GAGGGCCATC ATTGGGGTGC TGCTCAACTT AACTAATGAT
    AATGAATGGG GCAGCACCAA AACAGGAGAG CAGGATGGCT TGATTGGCAC AGCCATGAAT
    TGTGTGCTTC AGGTTCCAAA GTACCTACCT CAGGAGCAGA GATTTGATAT TCGAGTGCTG
    GGTTTAGGTC TACTGATAAA TCTAGTGGAA TATAGTGCTC GAAATCGGCA CTGTCTTGTC
    AACATGGAAA CATCATGTTC TTTTGATTCT TCCTTCTGTA GTGGAGAAGG AGATGATAGT
    TTAAGGATAG CTGGACAAGT TCATGCAGTT CAGGCTTTAG TGCAGCTGTT CCTTGAGCGA
    GAGCGAGCAG CACAGTTGGC AGAAAGTAAA ACAGATGAAC TGATCAAAGA TGCTCCCACC
    ACTCAGCATG ACAAGAGTGG GGAGTGGCAG GAGACGAGTG GGGAGATACA GTGGGTGTCA
    ACTGAGAAGG CTGATGGTGC AGAAGAGAAA AATAAGAAGG AGGAGGACGA TGAAGAACTT
    GACCTCAATA AAGCCCTTCA GCATGCTGGC AAGCACATGG AGGATTGCAT TGTGGCCTCA
    TACACAGCAC TGCTTCTTGG GTGTCTCTGC CAGGAGAGTC CAATCAATGT GACCACTGTG
    CGGGAGTATC TGCCAGAAGG GGACTTTTCA ATAATGACAG AGATGCTCAA AAAATTCCTG
    AGCTTCATGA ATCTTACCTG TGCTGTTGGA ACCACGGGCC AGAAGTCCAT CTCTAGAGTG
    ATTGAGTACT TGGAACACTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12498
    Clone ID Related Accession (Same CDS sequence) XM_005226524.3 , XM_005193122.3 , XM_005226524.4
    Accession Version XM_005226524.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product wings apart-like protein homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226524.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAGGACT TGCCTGGTGT GCCTGAAAGT
    GTGAAGAAGC CTCCAAATAA ACAAGGAGAT AAATCAAAGG AAAATACCAG AAAGATTTTT
    AGTGGCCCCA AACGGTCTCC TACAAAAGCC GTATATAATG CTAGACATTG GAATCATCCA
    GACTCGGAAG AACTACCTGC ACCACCAGTA GTAAAACCTC AGAGTGTCAC AGTGAGGCTG
    TCTTCAAAGG AGCAAAATCA AAAAGATGAT GGAGTTTTTA AGGCTCCTGC ACCACCACCC
    AAAGTGATAA AAACTGTGAC AATACCTACT CAGCCCTACC AAGATATAGT TACTGCACTG
    AAATGCAGAA AAGAAGACAA AGAATTGTAT ACTGTTGTTC AGCACGTGAA GCACTTCAAT
    GATGTGGTGG AATTTGGTGA AAACCAAGAG TTCACTGATG ACATTGAGTA CTTGTTAAGT
    GGCTTAAAGA GCACACAGCC TCTAAACACA CGTTGCCTTA GTGTTATTAG CTTGGCTACT
    AAATGTGCCA TGCCCAGTTT TCGAATGCAC CTGAGAGCAC ATGGAATGGT AGCAATGGTC
    TTTAAAACCT TGGATGATTC CCAGCATCAT CAGAATCTGT CCCTTTGTAC AGCTGCCCTC
    ATGTACATTC TGAGTAGAGA TCGTTTAAAC ATGGATCTTG ATAGAGCTAG CCTAGATCTG
    ATGATTCGAC TCCTAGAACT GGAACAAGAT GCTTCTTCAG CTAAGCTGCT GAATGAAAAA
    GACATGAACA AAATCAAAGA AAAAATTCGA AGGCTCTGTG AAACTGTACA CAATAAGCAC
    CTTGATCTAG AAAATATAAC GACTGGGCAT TTAGCTATGG AGACTTTACT GTCCCTTACT
    TCTAAACGAG CAGGAGACTG GTTTAAAGAA GAACTACGGC TTTTGGGTGG TCTGGATCAT
    ATTGTAGATA AAGTGAAAGA ATGTGTGGAT CATTTAAGTA AAGATGAGGA TGAAGAGAAA
    TTAGTAGCCT CATTATGGGG AGCAGAGAGA TGTTTACGAG TTTTAGAAAG CGTAACAGTG
    CATAATCCAG AGAATCAAAG CTACTTGATT GCCTATAAAG ATTCACAACT CATTGTTTCG
    TCAGCTAAGG CATTACAACA TTGTGAAGAG CTGATTAAGC AGTATAACCG TGCTGAGAAC
    AGTATATGCT TACCTGACAG TAAGCCTCTG CCTCAGCAGA ATGTAACTAA CCACGTGGGC
    AAAGCAGTGG AGGACTGCAT GAGGGCCATC ATTGGGGTGC TGCTCAACTT AACTAATGAT
    AATGAATGGG GCAGCACCAA AACAGGAGAG CAGGATGGCT TGATTGGCAC AGCCATGAAT
    TGTGTGCTTC AGGTTCCAAA GTACCTACCT CAGGAGCAGA GATTTGATAT TCGAGTGCTG
    GGTTTAGGTC TACTGATAAA TCTAGTGGAA TATAGTGCTC GAAATCGGCA CTGTCTTGTC
    AACATGGAAA CATCATGTTC TTTTGATTCT TCCTTCTGTA GTGGAGAAGG AGATGATAGT
    TTAAGGATAG CTGGACAAGT TCATGCAGTT CAGGCTTTAG TGCAGCTGTT CCTTGAGCGA
    GAGCGAGCAG CACAGTTGGC AGAAAGTAAA ACAGATGAAC TGATCAAAGA TGCTCCCACC
    ACTCAGCATG ACAAGAGTGG GGAGTGGCAG GAGACGAGTG GGGAGATACA GTGGGTGTCA
    ACTGAGAAGG CTGATGGTGC AGAAGAGAAA AATAAGAAGG AGGAGGACGA TGAAGAACTT
    GACCTCAATA AAGCCCTTCA GCATGCTGGC AAGCACATGG AGGATTGCAT TGTGGCCTCA
    TACACAGCAC TGCTTCTTGG GTGTCTCTGC CAGGAGAGTC CAATCAATGT GACCACTGTG
    CGGGAGTATC TGCCAGAAGG GGACTTTTCA ATAATGACAG AGATGCTCAA AAAATTCCTG
    AGCTTCATGA ATCTTACCTG TGCTGTTGGA ACCACGGGCC AGAAGTCCAT CTCTAGAGTG
    ATTGAGTACT TGGAACACTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226581.1
    CDS484..4047
    Translation

    Target ORF information:

    RefSeq Version XM_005226524.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WAPL cohesin release factor (WAPL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226524.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACATCCA GATTTGGGAA AACATACAGT AGGAAAGGAG GAAATGGAAG TTCAAAGTTT 
    GATGAAGTCT TTTCCAACAA ACGGACCACC CTTAGCACAA AGTGGGGAGA AACCACATTT
    ATGGCTAAAT TAGGACAGAA GAGGCCCAAT TTCAAACCAG ATATCCAAGA AGTTCCGAAG
    AAACCTAAGA TAGAAGAAGA AAATACTGGA GATCCTTTTG GATTTGATAG TGATGATGAA
    TCTCTACCAG TTTCTTCAAA GAATTTAGCT AAAGGTTCCT CTTACACGGA ATCTAGTGAA
    GCTGCTCAGT TGGAAGAGGT CACATCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGTG
    ATGGGTGAAG ACAGTGTTGT ATCTGATAAA TGCTTACGTT TGGAGGATAC TTTGCTTGGG
    AAAGAAAAGA GCACAAACCG TGTCTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA
    TTAATGACTT CAGATAAAGT GGAGAACTTT AGTGAAGAAC ATGAAAAGAA TAGTCACCAT
    ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACACAATG CAGAAACTAC AGTGGCTTCT
    GGATACAAAG CTGATGAAAT CAAGGAAAAT GATACTTGGA ATTCCCAACT TGGGAAAAGG
    TCAGAATCGC CATCAGAAAC ATCATCAATC AAGGGATCTG TAAGAACTGG TTTATATGAA
    TGGGATAATG ATTTTGAAGA TAGCAGATCC GAAGATTGTA TTTTAAATCT GGATAGTGAT
    CCTCTTTTGG AGATGAAGGA TGAGGATTTT AAAAATCGGT TGGAAAATCT AAATGAAGCC
    ATTGAGGAAG ACATGATGCA AAGTGTTCTT AGGCCAAGCA ACTGTAGGAC GTACTGTAGG
    GCCAATAAAG CAAAATCCTC ACAAGGAGCG TCGAGTTTTG ATAAGCTAAT GGACGGCACC
    AGTCAGGCCT TAGCCAAAGC AAACAGTGAA TCTAGTAAAG ATGGCCTGAA TCAGGCAAAG
    AAGGGCAGTG TAAGCTGTGG GACCAGTTTT AGAGGGACGG TTGGACGGAC TAGAGATTAT
    ACTGTTTTAC ATCCATCTTG CTTGTCAGTT TGTAATGTTA CCATCCAGGA TACTATGGAA
    CGCAGCATGG ATGAGTTCAC TGCTTCAACT CCTGCAGATT TGGGAGAAGC TGGCCGGCTA
    AGAAAAAAAG CAGATATTGC AACATCTAAG ACCACTACTA GATTTCGACC TAGTAATACT
    AAATCTAAAA AGGATGTTAA ACTTGAATTT TTTGGTTTTG AAGATCATGA TGAGACAGGA
    GGTGATGAGG GAGGTTCTGG GAGTTCTAAC TACAAAATTA AATATTTTGG CTTTGATGAT
    CTCAGTGAAA GTGAAGATGA TGAAGATGAT GACTGTCAGG TGGAAAGAAA GACAAGCAAA
    AAAAGAACTA AAACAGCTCC ATCACCCTCC TTGCAGCCTC CTCCAGAAAG CAATGATAAT
    TCCCAGGACA GTCAGTCTAG TTCTAATAAT GCAGAGGACT TGCCTGGTGT GCCTGAAAGT
    GTGAAGAAGC CTCCAAATAA ACAAGGAGAT AAATCAAAGG AAAATACCAG AAAGATTTTT
    AGTGGCCCCA AACGGTCTCC TACAAAAGCC GTATATAATG CTAGACATTG GAATCATCCA
    GACTCGGAAG AACTACCTGC ACCACCAGTA GTAAAACCTC AGAGTGTCAC AGTGAGGCTG
    TCTTCAAAGG AGCAAAATCA AAAAGATGAT GGAGTTTTTA AGGCTCCTGC ACCACCACCC
    AAAGTGATAA AAACTGTGAC AATACCTACT CAGCCCTACC AAGATATAGT TACTGCACTG
    AAATGCAGAA AAGAAGACAA AGAATTGTAT ACTGTTGTTC AGCACGTGAA GCACTTCAAT
    GATGTGGTGG AATTTGGTGA AAACCAAGAG TTCACTGATG ACATTGAGTA CTTGTTAAGT
    GGCTTAAAGA GCACACAGCC TCTAAACACA CGTTGCCTTA GTGTTATTAG CTTGGCTACT
    AAATGTGCCA TGCCCAGTTT TCGAATGCAC CTGAGAGCAC ATGGAATGGT AGCAATGGTC
    TTTAAAACCT TGGATGATTC CCAGCATCAT CAGAATCTGT CCCTTTGTAC AGCTGCCCTC
    ATGTACATTC TGAGTAGAGA TCGTTTAAAC ATGGATCTTG ATAGAGCTAG CCTAGATCTG
    ATGATTCGAC TCCTAGAACT GGAACAAGAT GCTTCTTCAG CTAAGCTGCT GAATGAAAAA
    GACATGAACA AAATCAAAGA AAAAATTCGA AGGCTCTGTG AAACTGTACA CAATAAGCAC
    CTTGATCTAG AAAATATAAC GACTGGGCAT TTAGCTATGG AGACTTTACT GTCCCTTACT
    TCTAAACGAG CAGGAGACTG GTTTAAAGAA GAACTACGGC TTTTGGGTGG TCTGGATCAT
    ATTGTAGATA AAGTGAAAGA ATGTGTGGAT CATTTAAGTA AAGATGAGGA TGAAGAGAAA
    TTAGTAGCCT CATTATGGGG AGCAGAGAGA TGTTTACGAG TTTTAGAAAG CGTAACAGTG
    CATAATCCAG AGAATCAAAG CTACTTGATT GCCTATAAAG ATTCACAACT CATTGTTTCG
    TCAGCTAAGG CATTACAACA TTGTGAAGAG CTGATTAAGC AGTATAACCG TGCTGAGAAC
    AGTATATGCT TACCTGACAG TAAGCCTCTG CCTCAGCAGA ATGTAACTAA CCACGTGGGC
    AAAGCAGTGG AGGACTGCAT GAGGGCCATC ATTGGGGTGC TGCTCAACTT AACTAATGAT
    AATGAATGGG GCAGCACCAA AACAGGAGAG CAGGATGGCT TGATTGGCAC AGCCATGAAT
    TGTGTGCTTC AGGTTCCAAA GTACCTACCT CAGGAGCAGA GATTTGATAT TCGAGTGCTG
    GGTTTAGGTC TACTGATAAA TCTAGTGGAA TATAGTGCTC GAAATCGGCA CTGTCTTGTC
    AACATGGAAA CATCATGTTC TTTTGATTCT TCCTTCTGTA GTGGAGAAGG AGATGATAGT
    TTAAGGATAG CTGGACAAGT TCATGCAGTT CAGGCTTTAG TGCAGCTGTT CCTTGAGCGA
    GAGCGAGCAG CACAGTTGGC AGAAAGTAAA ACAGATGAAC TGATCAAAGA TGCTCCCACC
    ACTCAGCATG ACAAGAGTGG GGAGTGGCAG GAGACGAGTG GGGAGATACA GTGGGTGTCA
    ACTGAGAAGG CTGATGGTGC AGAAGAGAAA AATAAGAAGG AGGAGGACGA TGAAGAACTT
    GACCTCAATA AAGCCCTTCA GCATGCTGGC AAGCACATGG AGGATTGCAT TGTGGCCTCA
    TACACAGCAC TGCTTCTTGG GTGTCTCTGC CAGGAGAGTC CAATCAATGT GACCACTGTG
    CGGGAGTATC TGCCAGAAGG GGACTTTTCA ATAATGACAG AGATGCTCAA AAAATTCCTG
    AGCTTCATGA ATCTTACCTG TGCTGTTGGA ACCACGGGCC AGAAGTCCAT CTCTAGAGTG
    ATTGAGTACT TGGAACACTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.