RIMS1 cDNA ORF clone, Bos taurus(cattle)

The following RIMS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIMS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa06027 XM_005210652.4
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa06027 XM_005199345.3
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa06029 XM_003586450.5
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa06029 XM_003582588.4
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa06029 XM_024996906.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa91431 XM_015464739.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa91431 XM_015472763.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa91431 XM_024996905.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa22456 XM_015464735.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22456 XM_015472759.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22457 XM_015464737.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa22457 XM_015472761.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa52635 XM_015472751.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52635 XM_015464727.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52636 XM_015464730.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52636 XM_015472754.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52637 XM_015472755.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52637 XM_015464731.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52638 XM_015472760.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52638 XM_015464736.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52639 XM_015464732.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52639 XM_015472756.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52640 XM_015472758.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52640 XM_015464734.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52641 XM_015464738.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa52641 XM_015472762.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa237965 XM_024996908.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa237966 XM_024996909.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa237967 XM_024996910.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa91427 XM_015464726.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91427 XM_015472750.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91428 XM_015472752.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X39, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91428 XM_015464728.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91429 XM_015464729.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91429 XM_015472753.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91430 XM_015464733.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91430 XM_015472757.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91433 XM_015472766.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa91433 XM_015464742.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa237941 XM_024996883.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237942 XM_024996884.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237943 XM_024996885.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237944 XM_024996886.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237945 XM_024996887.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237946 XM_024996888.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237947 XM_024996889.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237948 XM_024996890.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237949 XM_024996891.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237950 XM_024996892.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237951 XM_024996893.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237952 XM_015472759.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237953 XM_024996894.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237954 XM_024996895.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237955 XM_024996896.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237956 XM_024996897.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237957 XM_024996898.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237958 XM_024996899.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237959 XM_024996900.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237960 XM_024996901.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237961 XM_024996902.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237962 XM_024996903.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237963 XM_024996904.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa237968 XM_024996912.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa91426 XM_015464724.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91426 XM_015472748.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91432 XM_015464741.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91432 XM_015472765.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa22452 XM_015464725.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa22452 XM_015472749.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52634 XM_015464723.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52634 XM_015472747.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52643 XM_015472764.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa52643 XM_015464740.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa237933 XM_015472747.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237934 XM_015472748.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237935 XM_015472749.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237936 XM_015472750.2
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237937 XM_024996879.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237938 XM_024996880.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237939 XM_024996881.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237940 XM_024996882.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237964 XM_024996907.1
Latest version!
Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
View Old Accession Versions >>
OBa06027 XM_005210652.3 Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa52635 XM_015472751.1 Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52639 XM_015472756.1 Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa91428 XM_015472752.1 Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa06029 XM_003586450.4 Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa06027
    Clone ID Related Accession (Same CDS sequence) XM_005210652.3 , XM_005199345.3 , XM_005210652.4
    Accession Version XM_005210652.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGACTT CCGGAAGAGC CATCACCAAG AGCACAAGCA TGAGCGGAGA GATGTACACA 
    CTGGAGCATA ATGATGGCAG CCAGTCGGAC ACAGCTGTGG GCACCGTTGG AGCAGGTGGG
    AAGAAGAGGA GGTCCAGCCT GAGTGCCAAG GTGGTTGCCA TAGTGTCTCG AAGGAGCAGA
    AGCACATCCC AGCTGAGCCA AACAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210709.1
    CDS82..999
    Translation

    Target ORF information:

    RefSeq Version XM_005210652.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210652.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGACTT CCGGAAGAGC CATCACCAAG AGCACAAGCA TGAGCGGAGA GATGTACACA 
    CTGGAGCATA ATGATGGCAG CCAGTCGGAC ACAGCTGTGG GCACCGTTGG AGCAGGTGGG
    AAGAAGAGGA GGTCCAGCCT GAGTGCCAAG GTGGTTGCCA TAGTGTCTCG AAGGAGCAGA
    AGCACATCCC AGCTGAGCCA AACAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06027
    Clone ID Related Accession (Same CDS sequence) XM_005210652.3 , XM_005199345.3 , XM_005210652.4
    Accession Version XM_005199345.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGACTT CCGGAAGAGC CATCACCAAG AGCACAAGCA TGAGCGGAGA GATGTACACA 
    CTGGAGCATA ATGATGGCAG CCAGTCGGAC ACAGCTGTGG GCACCGTTGG AGCAGGTGGG
    AAGAAGAGGA GGTCCAGCCT GAGTGCCAAG GTGGTTGCCA TAGTGTCTCG AAGGAGCAGA
    AGCACATCCC AGCTGAGCCA AACAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199402.1
    CDS82..999
    Translation

    Target ORF information:

    RefSeq Version XM_005199345.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199345.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGACTT CCGGAAGAGC CATCACCAAG AGCACAAGCA TGAGCGGAGA GATGTACACA 
    CTGGAGCATA ATGATGGCAG CCAGTCGGAC ACAGCTGTGG GCACCGTTGG AGCAGGTGGG
    AAGAAGAGGA GGTCCAGCCT GAGTGCCAAG GTGGTTGCCA TAGTGTCTCG AAGGAGCAGA
    AGCACATCCC AGCTGAGCCA AACAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06027
    Clone ID Related Accession (Same CDS sequence) XM_005210652.3 , XM_005199345.3 , XM_005210652.4
    Accession Version XM_005210652.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210652.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGACTT CCGGAAGAGC CATCACCAAG AGCACAAGCA TGAGCGGAGA GATGTACACA 
    CTGGAGCATA ATGATGGCAG CCAGTCGGAC ACAGCTGTGG GCACCGTTGG AGCAGGTGGG
    AAGAAGAGGA GGTCCAGCCT GAGTGCCAAG GTGGTTGCCA TAGTGTCTCG AAGGAGCAGA
    AGCACATCCC AGCTGAGCCA AACAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210709.1
    CDS25..942
    Translation

    Target ORF information:

    RefSeq Version XM_005210652.4
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210652.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGACTT CCGGAAGAGC CATCACCAAG AGCACAAGCA TGAGCGGAGA GATGTACACA 
    CTGGAGCATA ATGATGGCAG CCAGTCGGAC ACAGCTGTGG GCACCGTTGG AGCAGGTGGG
    AAGAAGAGGA GGTCCAGCCT GAGTGCCAAG GTGGTTGCCA TAGTGTCTCG AAGGAGCAGA
    AGCACATCCC AGCTGAGCCA AACAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06029
    Clone ID Related Accession (Same CDS sequence) XM_003582588.4 , XM_003586450.4 , XM_024996906.1 , XM_003586450.5
    Accession Version XM_003582588.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582588.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582636.1
    CDS197..856
    Translation

    Target ORF information:

    RefSeq Version XM_003582588.4
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582588.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06029
    Clone ID Related Accession (Same CDS sequence) XM_003582588.4 , XM_003586450.4 , XM_024996906.1 , XM_003586450.5
    Accession Version XM_003586450.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586450.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586498.1
    CDS197..856
    Translation

    Target ORF information:

    RefSeq Version XM_003586450.4
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586450.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06029
    Clone ID Related Accession (Same CDS sequence) XM_003582588.4 , XM_003586450.4 , XM_024996906.1 , XM_003586450.5
    Accession Version XM_024996906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852674.1
    CDS199..858
    Translation

    Target ORF information:

    RefSeq Version XM_024996906.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06029
    Clone ID Related Accession (Same CDS sequence) XM_003582588.4 , XM_003586450.4 , XM_024996906.1 , XM_003586450.5
    Accession Version XM_003586450.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586450.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586498.1
    CDS208..867
    Translation

    Target ORF information:

    RefSeq Version XM_003586450.5
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586450.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC 
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91431
    Clone ID Related Accession (Same CDS sequence) XM_015472763.1 , XM_024996905.1 , XM_015464739.1
    Accession Version XM_015472763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT 
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328249.1
    CDS175..1371
    Translation

    Target ORF information:

    RefSeq Version XM_015472763.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT 
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91431
    Clone ID Related Accession (Same CDS sequence) XM_015472763.1 , XM_024996905.1 , XM_015464739.1
    Accession Version XM_024996905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT 
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852673.1
    CDS103..1299
    Translation

    Target ORF information:

    RefSeq Version XM_024996905.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT 
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91431
    Clone ID Related Accession (Same CDS sequence) XM_015472763.1 , XM_024996905.1 , XM_015464739.1
    Accession Version XM_015464739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT 
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320225.1
    CDS175..1371
    Translation

    Target ORF information:

    RefSeq Version XM_015464739.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT 
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22456
    Clone ID Related Accession (Same CDS sequence) XM_015464735.1 , XM_015472759.1
    Accession Version XM_015464735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320221.1
    CDS144..2693
    Translation

    Target ORF information:

    RefSeq Version XM_015464735.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22456
    Clone ID Related Accession (Same CDS sequence) XM_015464735.1 , XM_015472759.1
    Accession Version XM_015472759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328245.1
    CDS144..2693
    Translation

    Target ORF information:

    RefSeq Version XM_015472759.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22457
    Clone ID Related Accession (Same CDS sequence) XM_015472761.1 , XM_015464737.1
    Accession Version XM_015472761.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328247.1
    CDS144..2426
    Translation

    Target ORF information:

    RefSeq Version XM_015472761.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22457
    Clone ID Related Accession (Same CDS sequence) XM_015472761.1 , XM_015464737.1
    Accession Version XM_015464737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320223.1
    CDS144..2426
    Translation

    Target ORF information:

    RefSeq Version XM_015464737.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52635
    Clone ID Related Accession (Same CDS sequence) XM_015464727.1 , XM_015472751.1 , XM_015472751.2
    Accession Version XM_015464727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGGT TCTCCCACCA TGTCTTTCTA GAAGGGGATA CGCAGCCGCG
    AGAGCGACTG ATCAGCCAGT CCTTAGGGGG AAACATCCCG CTCGCTCCCG CTCGAGCGAG
    CACCCTAGTG TCAGAACACT CTGCTCTATG CACCACCTTG CCCCCGGAGG GTCCGCGCCA
    CCTTCTCCGC TCCTGACAAG GACGCACCGG CAGGCGAGCC CCACCCGGTC CCCGCCCATG
    GACGCCGCCT TCAGCAGCCG CAGGGGCAGG CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC
    AGCATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTG
    CATCGATTTA AGCAGACAAC AGGGTCTGGT TCTAGTCAAG AACTTGATCG CGAGCAATAT
    TCCAAGTATA ACGCACACAC AGATCAGTAC AGAAGCTGCG ACAACGTCTC CACCAGATCC
    TCAGACAGCG ACGTCAGCGA CGCGTCTGCC GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC
    AGCAGCACCA GCTTCCTGTC CGAGCAGTCT GAGCGGCCTC GGGGCAGGAT CAGTTCATTT
    ACCCCTAAAA TGCAAGGCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320213.1
    CDS144..3125
    Translation

    Target ORF information:

    RefSeq Version XM_015464727.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGGT TCTCCCACCA TGTCTTTCTA GAAGGGGATA CGCAGCCGCG
    AGAGCGACTG ATCAGCCAGT CCTTAGGGGG AAACATCCCG CTCGCTCCCG CTCGAGCGAG
    CACCCTAGTG TCAGAACACT CTGCTCTATG CACCACCTTG CCCCCGGAGG GTCCGCGCCA
    CCTTCTCCGC TCCTGACAAG GACGCACCGG CAGGCGAGCC CCACCCGGTC CCCGCCCATG
    GACGCCGCCT TCAGCAGCCG CAGGGGCAGG CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC
    AGCATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTG
    CATCGATTTA AGCAGACAAC AGGGTCTGGT TCTAGTCAAG AACTTGATCG CGAGCAATAT
    TCCAAGTATA ACGCACACAC AGATCAGTAC AGAAGCTGCG ACAACGTCTC CACCAGATCC
    TCAGACAGCG ACGTCAGCGA CGCGTCTGCC GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC
    AGCAGCACCA GCTTCCTGTC CGAGCAGTCT GAGCGGCCTC GGGGCAGGAT CAGTTCATTT
    ACCCCTAAAA TGCAAGGCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52635
    Clone ID Related Accession (Same CDS sequence) XM_015464727.1 , XM_015472751.1 , XM_015472751.2
    Accession Version XM_015472751.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGGT TCTCCCACCA TGTCTTTCTA GAAGGGGATA CGCAGCCGCG
    AGAGCGACTG ATCAGCCAGT CCTTAGGGGG AAACATCCCG CTCGCTCCCG CTCGAGCGAG
    CACCCTAGTG TCAGAACACT CTGCTCTATG CACCACCTTG CCCCCGGAGG GTCCGCGCCA
    CCTTCTCCGC TCCTGACAAG GACGCACCGG CAGGCGAGCC CCACCCGGTC CCCGCCCATG
    GACGCCGCCT TCAGCAGCCG CAGGGGCAGG CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC
    AGCATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTG
    CATCGATTTA AGCAGACAAC AGGGTCTGGT TCTAGTCAAG AACTTGATCG CGAGCAATAT
    TCCAAGTATA ACGCACACAC AGATCAGTAC AGAAGCTGCG ACAACGTCTC CACCAGATCC
    TCAGACAGCG ACGTCAGCGA CGCGTCTGCC GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC
    AGCAGCACCA GCTTCCTGTC CGAGCAGTCT GAGCGGCCTC GGGGCAGGAT CAGTTCATTT
    ACCCCTAAAA TGCAAGGCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328237.1
    CDS144..3125
    Translation

    Target ORF information:

    RefSeq Version XM_015472751.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGGT TCTCCCACCA TGTCTTTCTA GAAGGGGATA CGCAGCCGCG
    AGAGCGACTG ATCAGCCAGT CCTTAGGGGG AAACATCCCG CTCGCTCCCG CTCGAGCGAG
    CACCCTAGTG TCAGAACACT CTGCTCTATG CACCACCTTG CCCCCGGAGG GTCCGCGCCA
    CCTTCTCCGC TCCTGACAAG GACGCACCGG CAGGCGAGCC CCACCCGGTC CCCGCCCATG
    GACGCCGCCT TCAGCAGCCG CAGGGGCAGG CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC
    AGCATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTG
    CATCGATTTA AGCAGACAAC AGGGTCTGGT TCTAGTCAAG AACTTGATCG CGAGCAATAT
    TCCAAGTATA ACGCACACAC AGATCAGTAC AGAAGCTGCG ACAACGTCTC CACCAGATCC
    TCAGACAGCG ACGTCAGCGA CGCGTCTGCC GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC
    AGCAGCACCA GCTTCCTGTC CGAGCAGTCT GAGCGGCCTC GGGGCAGGAT CAGTTCATTT
    ACCCCTAAAA TGCAAGGCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52635
    Clone ID Related Accession (Same CDS sequence) XM_015464727.1 , XM_015472751.1 , XM_015472751.2
    Accession Version XM_015472751.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGGT TCTCCCACCA TGTCTTTCTA GAAGGGGATA CGCAGCCGCG
    AGAGCGACTG ATCAGCCAGT CCTTAGGGGG AAACATCCCG CTCGCTCCCG CTCGAGCGAG
    CACCCTAGTG TCAGAACACT CTGCTCTATG CACCACCTTG CCCCCGGAGG GTCCGCGCCA
    CCTTCTCCGC TCCTGACAAG GACGCACCGG CAGGCGAGCC CCACCCGGTC CCCGCCCATG
    GACGCCGCCT TCAGCAGCCG CAGGGGCAGG CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC
    AGCATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTG
    CATCGATTTA AGCAGACAAC AGGGTCTGGT TCTAGTCAAG AACTTGATCG CGAGCAATAT
    TCCAAGTATA ACGCACACAC AGATCAGTAC AGAAGCTGCG ACAACGTCTC CACCAGATCC
    TCAGACAGCG ACGTCAGCGA CGCGTCTGCC GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC
    AGCAGCACCA GCTTCCTGTC CGAGCAGTCT GAGCGGCCTC GGGGCAGGAT CAGTTCATTT
    ACCCCTAAAA TGCAAGGCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328237.1
    CDS162..3143
    Translation

    Target ORF information:

    RefSeq Version XM_015472751.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472751.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGGT TCTCCCACCA TGTCTTTCTA GAAGGGGATA CGCAGCCGCG
    AGAGCGACTG ATCAGCCAGT CCTTAGGGGG AAACATCCCG CTCGCTCCCG CTCGAGCGAG
    CACCCTAGTG TCAGAACACT CTGCTCTATG CACCACCTTG CCCCCGGAGG GTCCGCGCCA
    CCTTCTCCGC TCCTGACAAG GACGCACCGG CAGGCGAGCC CCACCCGGTC CCCGCCCATG
    GACGCCGCCT TCAGCAGCCG CAGGGGCAGG CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC
    AGCATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTG
    CATCGATTTA AGCAGACAAC AGGGTCTGGT TCTAGTCAAG AACTTGATCG CGAGCAATAT
    TCCAAGTATA ACGCACACAC AGATCAGTAC AGAAGCTGCG ACAACGTCTC CACCAGATCC
    TCAGACAGCG ACGTCAGCGA CGCGTCTGCC GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC
    AGCAGCACCA GCTTCCTGTC CGAGCAGTCT GAGCGGCCTC GGGGCAGGAT CAGTTCATTT
    ACCCCTAAAA TGCAAGGCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52636
    Clone ID Related Accession (Same CDS sequence) XM_015472754.1 , XM_015464730.1
    Accession Version XM_015472754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328240.1
    CDS144..2969
    Translation

    Target ORF information:

    RefSeq Version XM_015472754.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52636
    Clone ID Related Accession (Same CDS sequence) XM_015472754.1 , XM_015464730.1
    Accession Version XM_015464730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320216.1
    CDS144..2969
    Translation

    Target ORF information:

    RefSeq Version XM_015464730.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52637
    Clone ID Related Accession (Same CDS sequence) XM_015464731.1 , XM_015472755.1
    Accession Version XM_015464731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320217.1
    CDS144..2942
    Translation

    Target ORF information:

    RefSeq Version XM_015464731.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52637
    Clone ID Related Accession (Same CDS sequence) XM_015464731.1 , XM_015472755.1
    Accession Version XM_015472755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328241.1
    CDS144..2942
    Translation

    Target ORF information:

    RefSeq Version XM_015472755.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGCACT CCAGAAAGTC TGAAAGATCT AGCATCCAAA GCCAGACTAG GAAAGGCACT
    GCCTCTGATG CAGAAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52638
    Clone ID Related Accession (Same CDS sequence) XM_015464736.1 , XM_015472760.1
    Accession Version XM_015464736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320222.1
    CDS144..2939
    Translation

    Target ORF information:

    RefSeq Version XM_015464736.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52638
    Clone ID Related Accession (Same CDS sequence) XM_015464736.1 , XM_015472760.1
    Accession Version XM_015472760.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328246.1
    CDS144..2939
    Translation

    Target ORF information:

    RefSeq Version XM_015472760.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52639
    Clone ID Related Accession (Same CDS sequence) XM_015472756.1 , XM_015472756.2 , XM_015464732.1
    Accession Version XM_015472756.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328242.1
    CDS144..2870
    Translation

    Target ORF information:

    RefSeq Version XM_015472756.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52639
    Clone ID Related Accession (Same CDS sequence) XM_015472756.1 , XM_015472756.2 , XM_015464732.1
    Accession Version XM_015472756.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328242.1
    CDS161..2887
    Translation

    Target ORF information:

    RefSeq Version XM_015472756.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472756.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52639
    Clone ID Related Accession (Same CDS sequence) XM_015472756.1 , XM_015472756.2 , XM_015464732.1
    Accession Version XM_015464732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320218.1
    CDS144..2870
    Translation

    Target ORF information:

    RefSeq Version XM_015464732.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52640
    Clone ID Related Accession (Same CDS sequence) XM_015464734.1 , XM_015472758.1
    Accession Version XM_015464734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320220.1
    CDS144..2717
    Translation

    Target ORF information:

    RefSeq Version XM_015464734.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52640
    Clone ID Related Accession (Same CDS sequence) XM_015464734.1 , XM_015472758.1
    Accession Version XM_015472758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328244.1
    CDS144..2717
    Translation

    Target ORF information:

    RefSeq Version XM_015472758.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52641
    Clone ID Related Accession (Same CDS sequence) XM_015472762.1 , XM_015464738.1
    Accession Version XM_015472762.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGAG
    TCAGGCCACA AGAAGTTGAA AAGCACCATC CAGAGAAGCA CTGAGACAGG AATGGCAGCT
    GAGATGAGGA AGATGGTGAG GCAGCCAAGC CGGGAGTCCA CGGACGGCAG CATCAACAGC
    TACAGCTCCG AGGGAAATTT AATATTTCCT GGAGTGAGAC TAGGCGCAGA TAGTCAATTC
    AGTGATTTTC TTGATGGATT GGGACCAGCC CAGCTTGTTG GCCGCCAAAC CCTTGCTACC
    CCTGCAATGG GTGATATACA GATAGGAATG GAGGATAAAA AGGGCCAGTT AGAAGTTGAA
    GTGATTCGAG CACGAAGCCT CACGCAGAAA CCCGGCTCCA AATCCACACC TGCTCCTTAT
    GTCAAAGTGT ATCTTTTGGA GAATGGGGCC TGCATAGCCA AGAAGAAGAC AAGAATTGCA
    CGAAAGACCC TTGACCCTTT GTACCAACAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT
    AAAGTCCTTC AGGTGATTGT CTGGGGGGAC TACGGCAGAA TGGACCACAA ATGCTTCATG
    GGGGTGGCTC AGATCTTGTT GGAGGAACTC GATCTTTCCA GCATGGTCAT CGGCTGGTAC
    AAGCTGTTCC CACCGTCATC ACTGGTCGAC CCCACGCTCA CCCCTCTGAC CCGCCGGGCT
    TCCCAGTCAT CTCTGGAAAG TTCCACTGGG CCCCCCTGCA TCCGGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328248.1
    CDS144..2354
    Translation

    Target ORF information:

    RefSeq Version XM_015472762.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGAG
    TCAGGCCACA AGAAGTTGAA AAGCACCATC CAGAGAAGCA CTGAGACAGG AATGGCAGCT
    GAGATGAGGA AGATGGTGAG GCAGCCAAGC CGGGAGTCCA CGGACGGCAG CATCAACAGC
    TACAGCTCCG AGGGAAATTT AATATTTCCT GGAGTGAGAC TAGGCGCAGA TAGTCAATTC
    AGTGATTTTC TTGATGGATT GGGACCAGCC CAGCTTGTTG GCCGCCAAAC CCTTGCTACC
    CCTGCAATGG GTGATATACA GATAGGAATG GAGGATAAAA AGGGCCAGTT AGAAGTTGAA
    GTGATTCGAG CACGAAGCCT CACGCAGAAA CCCGGCTCCA AATCCACACC TGCTCCTTAT
    GTCAAAGTGT ATCTTTTGGA GAATGGGGCC TGCATAGCCA AGAAGAAGAC AAGAATTGCA
    CGAAAGACCC TTGACCCTTT GTACCAACAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT
    AAAGTCCTTC AGGTGATTGT CTGGGGGGAC TACGGCAGAA TGGACCACAA ATGCTTCATG
    GGGGTGGCTC AGATCTTGTT GGAGGAACTC GATCTTTCCA GCATGGTCAT CGGCTGGTAC
    AAGCTGTTCC CACCGTCATC ACTGGTCGAC CCCACGCTCA CCCCTCTGAC CCGCCGGGCT
    TCCCAGTCAT CTCTGGAAAG TTCCACTGGG CCCCCCTGCA TCCGGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52641
    Clone ID Related Accession (Same CDS sequence) XM_015472762.1 , XM_015464738.1
    Accession Version XM_015464738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGAG
    TCAGGCCACA AGAAGTTGAA AAGCACCATC CAGAGAAGCA CTGAGACAGG AATGGCAGCT
    GAGATGAGGA AGATGGTGAG GCAGCCAAGC CGGGAGTCCA CGGACGGCAG CATCAACAGC
    TACAGCTCCG AGGGAAATTT AATATTTCCT GGAGTGAGAC TAGGCGCAGA TAGTCAATTC
    AGTGATTTTC TTGATGGATT GGGACCAGCC CAGCTTGTTG GCCGCCAAAC CCTTGCTACC
    CCTGCAATGG GTGATATACA GATAGGAATG GAGGATAAAA AGGGCCAGTT AGAAGTTGAA
    GTGATTCGAG CACGAAGCCT CACGCAGAAA CCCGGCTCCA AATCCACACC TGCTCCTTAT
    GTCAAAGTGT ATCTTTTGGA GAATGGGGCC TGCATAGCCA AGAAGAAGAC AAGAATTGCA
    CGAAAGACCC TTGACCCTTT GTACCAACAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT
    AAAGTCCTTC AGGTGATTGT CTGGGGGGAC TACGGCAGAA TGGACCACAA ATGCTTCATG
    GGGGTGGCTC AGATCTTGTT GGAGGAACTC GATCTTTCCA GCATGGTCAT CGGCTGGTAC
    AAGCTGTTCC CACCGTCATC ACTGGTCGAC CCCACGCTCA CCCCTCTGAC CCGCCGGGCT
    TCCCAGTCAT CTCTGGAAAG TTCCACTGGG CCCCCCTGCA TCCGGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320224.1
    CDS144..2354
    Translation

    Target ORF information:

    RefSeq Version XM_015464738.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGAG
    TCAGGCCACA AGAAGTTGAA AAGCACCATC CAGAGAAGCA CTGAGACAGG AATGGCAGCT
    GAGATGAGGA AGATGGTGAG GCAGCCAAGC CGGGAGTCCA CGGACGGCAG CATCAACAGC
    TACAGCTCCG AGGGAAATTT AATATTTCCT GGAGTGAGAC TAGGCGCAGA TAGTCAATTC
    AGTGATTTTC TTGATGGATT GGGACCAGCC CAGCTTGTTG GCCGCCAAAC CCTTGCTACC
    CCTGCAATGG GTGATATACA GATAGGAATG GAGGATAAAA AGGGCCAGTT AGAAGTTGAA
    GTGATTCGAG CACGAAGCCT CACGCAGAAA CCCGGCTCCA AATCCACACC TGCTCCTTAT
    GTCAAAGTGT ATCTTTTGGA GAATGGGGCC TGCATAGCCA AGAAGAAGAC AAGAATTGCA
    CGAAAGACCC TTGACCCTTT GTACCAACAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT
    AAAGTCCTTC AGGTGATTGT CTGGGGGGAC TACGGCAGAA TGGACCACAA ATGCTTCATG
    GGGGTGGCTC AGATCTTGTT GGAGGAACTC GATCTTTCCA GCATGGTCAT CGGCTGGTAC
    AAGCTGTTCC CACCGTCATC ACTGGTCGAC CCCACGCTCA CCCCTCTGAC CCGCCGGGCT
    TCCCAGTCAT CTCTGGAAAG TTCCACTGGG CCCCCCTGCA TCCGGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237965
    Clone ID Related Accession (Same CDS sequence) XM_024996908.1
    Accession Version XM_024996908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGGTTCT CCCACCATGT CTTTCTAGAA GGGGATACGC AGCCGCGAGA
    GCGACTGATC AGCCAGTCCT TAGGGGGAAA CATCCCGCTC GCTCCCGCTC GAGCGAGCAC
    CCTAGTGTCA GAACACTCTG CTCTATGCAC CACCTTGCCC CCGGAGGGTC CGCGCCACCT
    TCTCCGCTCC TGACAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852676.1
    CDS162..3140
    Translation

    Target ORF information:

    RefSeq Version XM_024996908.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGGTTCT CCCACCATGT CTTTCTAGAA GGGGATACGC AGCCGCGAGA
    GCGACTGATC AGCCAGTCCT TAGGGGGAAA CATCCCGCTC GCTCCCGCTC GAGCGAGCAC
    CCTAGTGTCA GAACACTCTG CTCTATGCAC CACCTTGCCC CCGGAGGGTC CGCGCCACCT
    TCTCCGCTCC TGACAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237966
    Clone ID Related Accession (Same CDS sequence) XM_024996909.1
    Accession Version XM_024996909.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X39
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGACATCTTG TTAGGCACTA TAAAACATTA
    CCGCCTAAGA TGCCCTTATT AGAGACCTGT TCTCACTGGG ATATTTACAG CTCAATTCTG
    CCTGCACGTA CCAAAACCCA AACCCTTCAC CATGAATGTT TTCACTCGAG AGCACTGAGG
    TTTACTGATG AGATTCTGGT TGGTGACCTG CAGCCCTCCC TTGACAGGGC GAGGAGTGCT
    AGTACCAACT GCTTGAGACC AGATGCTAGT TTGCATTCAC CAGAACGAGA AAGGCACTCC
    AGAAAGTCTG AAAGATCTAG CATCCAAAGC CAGACTAGGA AAGGCACTGC CTCTGATGCA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852677.1
    CDS162..3128
    Translation

    Target ORF information:

    RefSeq Version XM_024996909.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGACATCTTG TTAGGCACTA TAAAACATTA
    CCGCCTAAGA TGCCCTTATT AGAGACCTGT TCTCACTGGG ATATTTACAG CTCAATTCTG
    CCTGCACGTA CCAAAACCCA AACCCTTCAC CATGAATGTT TTCACTCGAG AGCACTGAGG
    TTTACTGATG AGATTCTGGT TGGTGACCTG CAGCCCTCCC TTGACAGGGC GAGGAGTGCT
    AGTACCAACT GCTTGAGACC AGATGCTAGT TTGCATTCAC CAGAACGAGA AAGGCACTCC
    AGAAAGTCTG AAAGATCTAG CATCCAAAGC CAGACTAGGA AAGGCACTGC CTCTGATGCA
    GAAAGGACGC ACCGGCAGGC GAGCCCCACC CGGTCCCCGC CCATGGACGC CGCCTTCAGC
    AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG CCCGTGCGAA GCGGCAGCAT AGAACAAGCA
    AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTGCATCG ATTTAAGCAG
    ACAACAGGGT CTGGTTCTAG TCAAGAACTT GATCGCGAGC AATATTCCAA GTATAACGCA
    CACACAGATC AGTACAGAAG CTGCGACAAC GTCTCCACCA GATCCTCAGA CAGCGACGTC
    AGCGACGCGT CTGCCGTGTC CCGAACCAGC AGTGCCTCCC GCCTCAGCAG CACCAGCTTC
    CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC AGGATCAGTT CATTTACCCC TAAAATGCAA
    GGCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237967
    Clone ID Related Accession (Same CDS sequence) XM_024996910.1
    Accession Version XM_024996910.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X40
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGA CGCACCGGCA GGCGAGCCCC
    ACCCGGTCCC CGCCCATGGA CGCCGCCTTC AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG
    GTGCCCGTGC GAAGCGGCAG CATAGAACAA GCAAGCTTAG TAGTGGAGGA GCGAACGAGA
    CAGATGAAAA TGAAAGTGCA TCGATTTAAG CAGACAACAG GGTCTGGTTC TAGTCAAGAA
    CTTGATCGCG AGCAATATTC CAAGTATAAC GCACACACAG ATCAGTACAG AAGCTGCGAC
    AACGTCTCCA CCAGATCCTC AGACAGCGAC GTCAGCGACG CGTCTGCCGT GTCCCGAACC
    AGCAGTGCCT CCCGCCTCAG CAGCACCAGC TTCCTGTCCG AGCAGTCTGA GCGGCCTCGG
    GGCAGGATCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852678.1
    CDS161..3013
    Translation

    Target ORF information:

    RefSeq Version XM_024996910.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGA CGCACCGGCA GGCGAGCCCC
    ACCCGGTCCC CGCCCATGGA CGCCGCCTTC AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG
    GTGCCCGTGC GAAGCGGCAG CATAGAACAA GCAAGCTTAG TAGTGGAGGA GCGAACGAGA
    CAGATGAAAA TGAAAGTGCA TCGATTTAAG CAGACAACAG GGTCTGGTTC TAGTCAAGAA
    CTTGATCGCG AGCAATATTC CAAGTATAAC GCACACACAG ATCAGTACAG AAGCTGCGAC
    AACGTCTCCA CCAGATCCTC AGACAGCGAC GTCAGCGACG CGTCTGCCGT GTCCCGAACC
    AGCAGTGCCT CCCGCCTCAG CAGCACCAGC TTCCTGTCCG AGCAGTCTGA GCGGCCTCGG
    GGCAGGATCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91427
    Clone ID Related Accession (Same CDS sequence) XM_015472750.1 , XM_015464726.1
    Accession Version XM_015472750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAATAG GTTATAGGTC TAGCACTCGA
    GAAAGCAAAC CTTCGACATT AACCGTGCCA GAGCAGCAGA GAACAACTCA TCACCGCTCA
    CGGTCGGTGT CTCCTCATCG CGGCGACGAT CAGGGGAGGC CACGGTCACG TTTACCAAAT
    GTGCCATTAC AGAGGAGTTT AGATGAAATT CATCCAACAA GAAGATCACG TTCTCCAACC
    AGACACCGTG ATGCCTCCCG AAGCCCAATT GATCAGAGAA TCAGAGATGT GGACAGTCAG
    TGTTTATCAG AACAAGACAG TGAGCTTCTC ATGCTTCCCA GAGCAAAACG AGGACGAAGT
    GCAGAATGCC TACACACCAC CAGTGACCTG CAGCCCTCCC TTGACAGGGC GAGGAGTGCT
    AGTACCAACT GCTTGAGACC AGATGCTAGT TTGCATTCAC CAGAACGAGA AAGGGGTAGA
    TGGTCCCCCT CCCTAGATAG GAGACGACCT CCTAGCCCCA GGATTCAAAT CCAGCATGCA
    TCTCCGGAGA ATGACAGGCA CTCCAGAAAG TCTGAAAGAT CTAGCATCCA AAGCCAGACT
    AGGAAAGGCA CTGCCTCTGA TGCAGAAAGG GTTCTCCCAC CATGTCTTTC TAGAAGGGGA
    TACGCAGCCG CGAGAGCGAC TGATCAGCCA GTCCTTAGGG GGAAACATCC CGCTCGCTCC
    CGCTCGAGCG AGCACCCTAG TGTCAGAACA CTCTGCTCTA TGCACCACCT TGCCCCCGGA
    GGGTCCGCGC CACCTTCTCC GCTCCTGACA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328236.1
    CDS144..3137
    Translation

    Target ORF information:

    RefSeq Version XM_015472750.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAATAG GTTATAGGTC TAGCACTCGA
    GAAAGCAAAC CTTCGACATT AACCGTGCCA GAGCAGCAGA GAACAACTCA TCACCGCTCA
    CGGTCGGTGT CTCCTCATCG CGGCGACGAT CAGGGGAGGC CACGGTCACG TTTACCAAAT
    GTGCCATTAC AGAGGAGTTT AGATGAAATT CATCCAACAA GAAGATCACG TTCTCCAACC
    AGACACCGTG ATGCCTCCCG AAGCCCAATT GATCAGAGAA TCAGAGATGT GGACAGTCAG
    TGTTTATCAG AACAAGACAG TGAGCTTCTC ATGCTTCCCA GAGCAAAACG AGGACGAAGT
    GCAGAATGCC TACACACCAC CAGTGACCTG CAGCCCTCCC TTGACAGGGC GAGGAGTGCT
    AGTACCAACT GCTTGAGACC AGATGCTAGT TTGCATTCAC CAGAACGAGA AAGGGGTAGA
    TGGTCCCCCT CCCTAGATAG GAGACGACCT CCTAGCCCCA GGATTCAAAT CCAGCATGCA
    TCTCCGGAGA ATGACAGGCA CTCCAGAAAG TCTGAAAGAT CTAGCATCCA AAGCCAGACT
    AGGAAAGGCA CTGCCTCTGA TGCAGAAAGG GTTCTCCCAC CATGTCTTTC TAGAAGGGGA
    TACGCAGCCG CGAGAGCGAC TGATCAGCCA GTCCTTAGGG GGAAACATCC CGCTCGCTCC
    CGCTCGAGCG AGCACCCTAG TGTCAGAACA CTCTGCTCTA TGCACCACCT TGCCCCCGGA
    GGGTCCGCGC CACCTTCTCC GCTCCTGACA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91427
    Clone ID Related Accession (Same CDS sequence) XM_015472750.1 , XM_015464726.1
    Accession Version XM_015464726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAATAG GTTATAGGTC TAGCACTCGA
    GAAAGCAAAC CTTCGACATT AACCGTGCCA GAGCAGCAGA GAACAACTCA TCACCGCTCA
    CGGTCGGTGT CTCCTCATCG CGGCGACGAT CAGGGGAGGC CACGGTCACG TTTACCAAAT
    GTGCCATTAC AGAGGAGTTT AGATGAAATT CATCCAACAA GAAGATCACG TTCTCCAACC
    AGACACCGTG ATGCCTCCCG AAGCCCAATT GATCAGAGAA TCAGAGATGT GGACAGTCAG
    TGTTTATCAG AACAAGACAG TGAGCTTCTC ATGCTTCCCA GAGCAAAACG AGGACGAAGT
    GCAGAATGCC TACACACCAC CAGTGACCTG CAGCCCTCCC TTGACAGGGC GAGGAGTGCT
    AGTACCAACT GCTTGAGACC AGATGCTAGT TTGCATTCAC CAGAACGAGA AAGGGGTAGA
    TGGTCCCCCT CCCTAGATAG GAGACGACCT CCTAGCCCCA GGATTCAAAT CCAGCATGCA
    TCTCCGGAGA ATGACAGGCA CTCCAGAAAG TCTGAAAGAT CTAGCATCCA AAGCCAGACT
    AGGAAAGGCA CTGCCTCTGA TGCAGAAAGG GTTCTCCCAC CATGTCTTTC TAGAAGGGGA
    TACGCAGCCG CGAGAGCGAC TGATCAGCCA GTCCTTAGGG GGAAACATCC CGCTCGCTCC
    CGCTCGAGCG AGCACCCTAG TGTCAGAACA CTCTGCTCTA TGCACCACCT TGCCCCCGGA
    GGGTCCGCGC CACCTTCTCC GCTCCTGACA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320212.1
    CDS144..3137
    Translation

    Target ORF information:

    RefSeq Version XM_015464726.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAATAG GTTATAGGTC TAGCACTCGA
    GAAAGCAAAC CTTCGACATT AACCGTGCCA GAGCAGCAGA GAACAACTCA TCACCGCTCA
    CGGTCGGTGT CTCCTCATCG CGGCGACGAT CAGGGGAGGC CACGGTCACG TTTACCAAAT
    GTGCCATTAC AGAGGAGTTT AGATGAAATT CATCCAACAA GAAGATCACG TTCTCCAACC
    AGACACCGTG ATGCCTCCCG AAGCCCAATT GATCAGAGAA TCAGAGATGT GGACAGTCAG
    TGTTTATCAG AACAAGACAG TGAGCTTCTC ATGCTTCCCA GAGCAAAACG AGGACGAAGT
    GCAGAATGCC TACACACCAC CAGTGACCTG CAGCCCTCCC TTGACAGGGC GAGGAGTGCT
    AGTACCAACT GCTTGAGACC AGATGCTAGT TTGCATTCAC CAGAACGAGA AAGGGGTAGA
    TGGTCCCCCT CCCTAGATAG GAGACGACCT CCTAGCCCCA GGATTCAAAT CCAGCATGCA
    TCTCCGGAGA ATGACAGGCA CTCCAGAAAG TCTGAAAGAT CTAGCATCCA AAGCCAGACT
    AGGAAAGGCA CTGCCTCTGA TGCAGAAAGG GTTCTCCCAC CATGTCTTTC TAGAAGGGGA
    TACGCAGCCG CGAGAGCGAC TGATCAGCCA GTCCTTAGGG GGAAACATCC CGCTCGCTCC
    CGCTCGAGCG AGCACCCTAG TGTCAGAACA CTCTGCTCTA TGCACCACCT TGCCCCCGGA
    GGGTCCGCGC CACCTTCTCC GCTCCTGACA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91428
    Clone ID Related Accession (Same CDS sequence) XM_015464728.1 , XM_015472752.2 , XM_015472752.1
    Accession Version XM_015464728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320214.1
    CDS144..3026
    Translation

    Target ORF information:

    RefSeq Version XM_015464728.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91428
    Clone ID Related Accession (Same CDS sequence) XM_015464728.1 , XM_015472752.2 , XM_015472752.1
    Accession Version XM_015472752.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328238.1
    CDS161..3043
    Translation

    Target ORF information:

    RefSeq Version XM_015472752.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X39, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91428
    Clone ID Related Accession (Same CDS sequence) XM_015464728.1 , XM_015472752.2 , XM_015472752.1
    Accession Version XM_015472752.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328238.1
    CDS144..3026
    Translation

    Target ORF information:

    RefSeq Version XM_015472752.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91429
    Clone ID Related Accession (Same CDS sequence) XM_015464729.1 , XM_015472753.1
    Accession Version XM_015464729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320215.1
    CDS144..2972
    Translation

    Target ORF information:

    RefSeq Version XM_015464729.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91429
    Clone ID Related Accession (Same CDS sequence) XM_015464729.1 , XM_015472753.1
    Accession Version XM_015472753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328239.1
    CDS144..2972
    Translation

    Target ORF information:

    RefSeq Version XM_015472753.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91430
    Clone ID Related Accession (Same CDS sequence) XM_015472757.1 , XM_015464733.1
    Accession Version XM_015472757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328243.1
    CDS144..2789
    Translation

    Target ORF information:

    RefSeq Version XM_015472757.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91430
    Clone ID Related Accession (Same CDS sequence) XM_015472757.1 , XM_015464733.1
    Accession Version XM_015464733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320219.1
    CDS144..2789
    Translation

    Target ORF information:

    RefSeq Version XM_015464733.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91433
    Clone ID Related Accession (Same CDS sequence) XM_015464742.1 , XM_015472766.1
    Accession Version XM_015464742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320228.1
    CDS144..2690
    Translation

    Target ORF information:

    RefSeq Version XM_015464742.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91433
    Clone ID Related Accession (Same CDS sequence) XM_015464742.1 , XM_015472766.1
    Accession Version XM_015472766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328252.1
    CDS144..2690
    Translation

    Target ORF information:

    RefSeq Version XM_015472766.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237941
    Clone ID Related Accession (Same CDS sequence) XM_024996883.1
    Accession Version XM_024996883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGGTTCT CCCACCATGT CTTTCTAGAA GGGGATACGC AGCCGCGAGA
    GCGACTGATC AGCCAGTCCT TAGGGGGAAA CATCCCGCTC GCTCCCGCTC GAGCGAGCAC
    CCTAGTGTCA GAACACTCTG CTCTATGCAC CACCTTGCCC CCGGAGGGTC CGCGCCACCT
    TCTCCGCTCC TGACAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852651.1
    CDS6..4484
    Translation

    Target ORF information:

    RefSeq Version XM_024996883.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGGTTCT CCCACCATGT CTTTCTAGAA GGGGATACGC AGCCGCGAGA
    GCGACTGATC AGCCAGTCCT TAGGGGGAAA CATCCCGCTC GCTCCCGCTC GAGCGAGCAC
    CCTAGTGTCA GAACACTCTG CTCTATGCAC CACCTTGCCC CCGGAGGGTC CGCGCCACCT
    TCTCCGCTCC TGACAAGGAC GCACCGGCAG GCGAGCCCCA CCCGGTCCCC GCCCATGGAC
    GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC
    ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTGCAT
    CGATTTAAGC AGACAACAGG GTCTGGTTCT AGTCAAGAAC TTGATCGCGA GCAATATTCC
    AAGTATAACG CACACACAGA TCAGTACAGA AGCTGCGACA ACGTCTCCAC CAGATCCTCA
    GACAGCGACG TCAGCGACGC GTCTGCCGTG TCCCGAACCA GCAGTGCCTC CCGCCTCAGC
    AGCACCAGCT TCCTGTCCGA GCAGTCTGAG CGGCCTCGGG GCAGGATCAG TTCATTTACC
    CCTAAAATGC AAGGCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237942
    Clone ID Related Accession (Same CDS sequence) XM_024996884.1
    Accession Version XM_024996884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852652.1
    CDS6..4481
    Translation

    Target ORF information:

    RefSeq Version XM_024996884.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237943
    Clone ID Related Accession (Same CDS sequence) XM_024996885.1
    Accession Version XM_024996885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852653.1
    CDS6..4454
    Translation

    Target ORF information:

    RefSeq Version XM_024996885.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237944
    Clone ID Related Accession (Same CDS sequence) XM_024996886.1
    Accession Version XM_024996886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852654.1
    CDS6..4451
    Translation

    Target ORF information:

    RefSeq Version XM_024996886.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237945
    Clone ID Related Accession (Same CDS sequence) XM_024996887.1
    Accession Version XM_024996887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852655.1
    CDS6..4424
    Translation

    Target ORF information:

    RefSeq Version XM_024996887.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237946
    Clone ID Related Accession (Same CDS sequence) XM_024996888.1
    Accession Version XM_024996888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852656.1
    CDS5..4381
    Translation

    Target ORF information:

    RefSeq Version XM_024996888.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237947
    Clone ID Related Accession (Same CDS sequence) XM_024996889.1
    Accession Version XM_024996889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852657.1
    CDS5..4378
    Translation

    Target ORF information:

    RefSeq Version XM_024996889.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGTTCAT TTACCCCTAA AATGCAAGGC AGACGGATGG GGACTTCCGG AAGAGCCATC
    ACCAAGAGCA CAAGCATGAG CGGAGAGATG TACACACTGG AGCATAATGA TGGCAGCCAG
    TCGGACACAG CTGTGGGCAC CGTTGGAGCA GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT
    GCCAAGGTGG TTGCCATAGT GTCTCGAAGG AGCAGAAGCA CATCCCAGCT GAGCCAAACA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237948
    Clone ID Related Accession (Same CDS sequence) XM_024996890.1
    Accession Version XM_024996890.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGAC GGATGGGGAC TTCCGGAAGA GCCATCACCA AGAGCACAAG CATGAGCGGA
    GAGATGTACA CACTGGAGCA TAATGATGGC AGCCAGTCGG ACACAGCTGT GGGCACCGTT
    GGAGCAGGTG GGAAGAAGAG GAGGTCCAGC CTGAGTGCCA AGGTGGTTGC CATAGTGTCT
    CGAAGGAGCA GAAGCACATC CCAGCTGAGC CAAACAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852658.1
    CDS5..4354
    Translation

    Target ORF information:

    RefSeq Version XM_024996890.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996890.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGAC GGATGGGGAC TTCCGGAAGA GCCATCACCA AGAGCACAAG CATGAGCGGA
    GAGATGTACA CACTGGAGCA TAATGATGGC AGCCAGTCGG ACACAGCTGT GGGCACCGTT
    GGAGCAGGTG GGAAGAAGAG GAGGTCCAGC CTGAGTGCCA AGGTGGTTGC CATAGTGTCT
    CGAAGGAGCA GAAGCACATC CCAGCTGAGC CAAACAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237949
    Clone ID Related Accession (Same CDS sequence) XM_024996891.1
    Accession Version XM_024996891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852659.1
    CDS6..4352
    Translation

    Target ORF information:

    RefSeq Version XM_024996891.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG
    AAAATGAAAG TGCATCGATT TAAGCAGACA ACAGGGTCTG GTTCTAGTCA AGAACTTGAT
    CGCGAGCAAT ATTCCAAGTA TAACGCACAC ACAGATCAGT ACAGAAGCTG CGACAACGTC
    TCCACCAGAT CCTCAGACAG CGACGTCAGC GACGCGTCTG CCGTGTCCCG AACCAGCAGT
    GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG
    ATCAGACGGA TGGGGACTTC CGGAAGAGCC ATCACCAAGA GCACAAGCAT GAGCGGAGAG
    ATGTACACAC TGGAGCATAA TGATGGCAGC CAGTCGGACA CAGCTGTGGG CACCGTTGGA
    GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA
    AGGAGCAGAA GCACATCCCA GCTGAGCCAA ACAGAGTCAG GCCACAAGAA GTTGAAAAGC
    ACCATCCAGA GAAGCACTGA GACAGGAATG GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG
    CCAAGCCGGG AGTCCACGGA CGGCAGCATC AACAGCTACA GCTCCGAGGG AAATTTAATA
    TTTCCTGGAG TGAGACTAGG CGCAGATAGT CAATTCAGTG ATTTTCTTGA TGGATTGGGA
    CCAGCCCAGC TTGTTGGCCG CCAAACCCTT GCTACCCCTG CAATGGGTGA TATACAGATA
    GGAATGGAGG ATAAAAAGGG CCAGTTAGAA GTTGAAGTGA TTCGAGCACG AAGCCTCACG
    CAGAAACCCG GCTCCAAATC CACACCTGCT CCTTATGTCA AAGTGTATCT TTTGGAGAAT
    GGGGCCTGCA TAGCCAAGAA GAAGACAAGA ATTGCACGAA AGACCCTTGA CCCTTTGTAC
    CAACAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TCCTTCAGGT GATTGTCTGG
    GGGGACTACG GCAGAATGGA CCACAAATGC TTCATGGGGG TGGCTCAGAT CTTGTTGGAG
    GAACTCGATC TTTCCAGCAT GGTCATCGGC TGGTACAAGC TGTTCCCACC GTCATCACTG
    GTCGACCCCA CGCTCACCCC TCTGACCCGC CGGGCTTCCC AGTCATCTCT GGAAAGTTCC
    ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237950
    Clone ID Related Accession (Same CDS sequence) XM_024996892.1
    Accession Version XM_024996892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852660.1
    CDS5..4300
    Translation

    Target ORF information:

    RefSeq Version XM_024996892.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237951
    Clone ID Related Accession (Same CDS sequence) XM_024996893.1
    Accession Version XM_024996893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4293bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGCAAGCT TAGTAGTGGA GGAGCGAACG AGACAGATGA AAATGAAAGT GCATCGATTT
    AAGCAGACAA CAGGGTCTGG TTCTAGTCAA GAACTTGATC GCGAGCAATA TTCCAAGTAT
    AACGCACACA CAGATCAGTA CAGAAGCTGC GACAACGTCT CCACCAGATC CTCAGACAGC
    GACGTCAGCG ACGCGTCTGC CGTGTCCCGA ACCAGCAGTG CCTCCCGCCT CAGCAGCACC
    AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT CGGGGCAGGA TCAGTTCATT TACCCCTAAA
    ATGCAAGGCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852661.1
    CDS6..4298
    Translation

    Target ORF information:

    RefSeq Version XM_024996893.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGCAAGCT TAGTAGTGGA GGAGCGAACG AGACAGATGA AAATGAAAGT GCATCGATTT
    AAGCAGACAA CAGGGTCTGG TTCTAGTCAA GAACTTGATC GCGAGCAATA TTCCAAGTAT
    AACGCACACA CAGATCAGTA CAGAAGCTGC GACAACGTCT CCACCAGATC CTCAGACAGC
    GACGTCAGCG ACGCGTCTGC CGTGTCCCGA ACCAGCAGTG CCTCCCGCCT CAGCAGCACC
    AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT CGGGGCAGGA TCAGTTCATT TACCCCTAAA
    ATGCAAGGCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237952
    Clone ID Related Accession (Same CDS sequence) XM_015472759.2
    Accession Version XM_015472759.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGAGT CAGGCCACAA GAAGTTGAAA AGCACCATCC AGAGAAGCAC TGAGACAGGA
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328245.2
    CDS6..4205
    Translation

    Target ORF information:

    RefSeq Version XM_015472759.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472759.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGAGT CAGGCCACAA GAAGTTGAAA AGCACCATCC AGAGAAGCAC TGAGACAGGA
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237953
    Clone ID Related Accession (Same CDS sequence) XM_024996894.1
    Accession Version XM_024996894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGGT TCTCCCACCA
    TGTCTTTCTA GAAGGGGATA CGCAGCCGCG AGAGCGACTG ATCAGCCAGT CCTTAGGGGG
    AAACATCCCG CTCGCTCCCG CTCGAGCGAG CACCCTAGTG TCAGAACACT CTGCTCTATG
    CACCACCTTG CCCCCGGAGG GTCCGCGCCA CCTTCTCCGC TCCTGACAAG GACGCACCGG
    CAGGCGAGCC CCACCCGGTC CCCGCCCATG GACGCCGCCT TCAGCAGCCG CAGGGGCAGG
    CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC AGCATAGAAC AAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852662.1
    CDS6..4121
    Translation

    Target ORF information:

    RefSeq Version XM_024996894.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGGT TCTCCCACCA
    TGTCTTTCTA GAAGGGGATA CGCAGCCGCG AGAGCGACTG ATCAGCCAGT CCTTAGGGGG
    AAACATCCCG CTCGCTCCCG CTCGAGCGAG CACCCTAGTG TCAGAACACT CTGCTCTATG
    CACCACCTTG CCCCCGGAGG GTCCGCGCCA CCTTCTCCGC TCCTGACAAG GACGCACCGG
    CAGGCGAGCC CCACCCGGTC CCCGCCCATG GACGCCGCCT TCAGCAGCCG CAGGGGCAGG
    CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC AGCATAGAAC AAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237954
    Clone ID Related Accession (Same CDS sequence) XM_024996895.1
    Accession Version XM_024996895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852663.1
    CDS6..4118
    Translation

    Target ORF information:

    RefSeq Version XM_024996895.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237955
    Clone ID Related Accession (Same CDS sequence) XM_024996896.1
    Accession Version XM_024996896.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852664.1
    CDS5..4021
    Translation

    Target ORF information:

    RefSeq Version XM_024996896.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237956
    Clone ID Related Accession (Same CDS sequence) XM_024996897.1
    Accession Version XM_024996897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGGGTAGAT GGTCCCCCTC CCTAGATAGG
    AGACGACCTC CTAGCCCCAG GATTCAAATC CAGCATGCAT CTCCGGAGAA TGACAGGCAC
    TCCAGAAAGT CTGAAAGATC TAGCATCCAA AGCCAGACTA GGAAAGGCAC TGCCTCTGAT
    GCAGAAAGGA CGCACCGGCA GGCGAGCCCC ACCCGGTCCC CGCCCATGGA CGCCGCCTTC
    AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG GTGCCCGTGC GAAGCGGCAG CATAGAACAA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852665.1
    CDS5..4018
    Translation

    Target ORF information:

    RefSeq Version XM_024996897.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGGGTAGAT GGTCCCCCTC CCTAGATAGG
    AGACGACCTC CTAGCCCCAG GATTCAAATC CAGCATGCAT CTCCGGAGAA TGACAGGCAC
    TCCAGAAAGT CTGAAAGATC TAGCATCCAA AGCCAGACTA GGAAAGGCAC TGCCTCTGAT
    GCAGAAAGGA CGCACCGGCA GGCGAGCCCC ACCCGGTCCC CGCCCATGGA CGCCGCCTTC
    AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG GTGCCCGTGC GAAGCGGCAG CATAGAACAA
    GAGTCAGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGAA GCACTGAGAC AGGAATGGCA
    GCTGAGATGA GGAAGATGGT GAGGCAGCCA AGCCGGGAGT CCACGGACGG CAGCATCAAC
    AGCTACAGCT CCGAGGGAAA TTTAATATTT CCTGGAGTGA GACTAGGCGC AGATAGTCAA
    TTCAGTGATT TTCTTGATGG ATTGGGACCA GCCCAGCTTG TTGGCCGCCA AACCCTTGCT
    ACCCCTGCAA TGGGTGATAT ACAGATAGGA ATGGAGGATA AAAAGGGCCA GTTAGAAGTT
    GAAGTGATTC GAGCACGAAG CCTCACGCAG AAACCCGGCT CCAAATCCAC ACCTGCTCCT
    TATGTCAAAG TGTATCTTTT GGAGAATGGG GCCTGCATAG CCAAGAAGAA GACAAGAATT
    GCACGAAAGA CCCTTGACCC TTTGTACCAA CAGTCCCTGG TTTTTGATGA AAGTCCACAG
    GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG GACTACGGCA GAATGGACCA CAAATGCTTC
    ATGGGGGTGG CTCAGATCTT GTTGGAGGAA CTCGATCTTT CCAGCATGGT CATCGGCTGG
    TACAAGCTGT TCCCACCGTC ATCACTGGTC GACCCCACGC TCACCCCTCT GACCCGCCGG
    GCTTCCCAGT CATCTCTGGA AAGTTCCACT GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237957
    Clone ID Related Accession (Same CDS sequence) XM_024996898.1
    Accession Version XM_024996898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852666.1
    CDS5..3937
    Translation

    Target ORF information:

    RefSeq Version XM_024996898.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237958
    Clone ID Related Accession (Same CDS sequence) XM_024996899.1
    Accession Version XM_024996899.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852667.1
    CDS5..3934
    Translation

    Target ORF information:

    RefSeq Version XM_024996899.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996899.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGAGT CAGGCCACAA GAAGTTGAAA
    AGCACCATCC AGAGAAGCAC TGAGACAGGA ATGGCAGCTG AGATGAGGAA GATGGTGAGG
    CAGCCAAGCC GGGAGTCCAC GGACGGCAGC ATCAACAGCT ACAGCTCCGA GGGAAATTTA
    ATATTTCCTG GAGTGAGACT AGGCGCAGAT AGTCAATTCA GTGATTTTCT TGATGGATTG
    GGACCAGCCC AGCTTGTTGG CCGCCAAACC CTTGCTACCC CTGCAATGGG TGATATACAG
    ATAGGAATGG AGGATAAAAA GGGCCAGTTA GAAGTTGAAG TGATTCGAGC ACGAAGCCTC
    ACGCAGAAAC CCGGCTCCAA ATCCACACCT GCTCCTTATG TCAAAGTGTA TCTTTTGGAG
    AATGGGGCCT GCATAGCCAA GAAGAAGACA AGAATTGCAC GAAAGACCCT TGACCCTTTG
    TACCAACAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTCCTTCA GGTGATTGTC
    TGGGGGGACT ACGGCAGAAT GGACCACAAA TGCTTCATGG GGGTGGCTCA GATCTTGTTG
    GAGGAACTCG ATCTTTCCAG CATGGTCATC GGCTGGTACA AGCTGTTCCC ACCGTCATCA
    CTGGTCGACC CCACGCTCAC CCCTCTGACC CGCCGGGCTT CCCAGTCATC TCTGGAAAGT
    TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237959
    Clone ID Related Accession (Same CDS sequence) XM_024996900.1
    Accession Version XM_024996900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGAG TCAGGCCACA AGAAGTTGAA AAGCACCATC
    CAGAGAAGCA CTGAGACAGG AATGGCAGCT GAGATGAGGA AGATGGTGAG GCAGCCAAGC
    CGGGAGTCCA CGGACGGCAG CATCAACAGC TACAGCTCCG AGGGAAATTT AATATTTCCT
    GGAGTGAGAC TAGGCGCAGA TAGTCAATTC AGTGATTTTC TTGATGGATT GGGACCAGCC
    CAGCTTGTTG GCCGCCAAAC CCTTGCTACC CCTGCAATGG GTGATATACA GATAGGAATG
    GAGGATAAAA AGGGCCAGTT AGAAGTTGAA GTGATTCGAG CACGAAGCCT CACGCAGAAA
    CCCGGCTCCA AATCCACACC TGCTCCTTAT GTCAAAGTGT ATCTTTTGGA GAATGGGGCC
    TGCATAGCCA AGAAGAAGAC AAGAATTGCA CGAAAGACCC TTGACCCTTT GTACCAACAG
    TCCCTGGTTT TTGATGAAAG TCCACAGGGT AAAGTCCTTC AGGTGATTGT CTGGGGGGAC
    TACGGCAGAA TGGACCACAA ATGCTTCATG GGGGTGGCTC AGATCTTGTT GGAGGAACTC
    GATCTTTCCA GCATGGTCAT CGGCTGGTAC AAGCTGTTCC CACCGTCATC ACTGGTCGAC
    CCCACGCTCA CCCCTCTGAC CCGCCGGGCT TCCCAGTCAT CTCTGGAAAG TTCCACTGGG
    CCCCCCTGCA TCCGGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852668.1
    CDS5..3865
    Translation

    Target ORF information:

    RefSeq Version XM_024996900.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGAG TCAGGCCACA AGAAGTTGAA AAGCACCATC
    CAGAGAAGCA CTGAGACAGG AATGGCAGCT GAGATGAGGA AGATGGTGAG GCAGCCAAGC
    CGGGAGTCCA CGGACGGCAG CATCAACAGC TACAGCTCCG AGGGAAATTT AATATTTCCT
    GGAGTGAGAC TAGGCGCAGA TAGTCAATTC AGTGATTTTC TTGATGGATT GGGACCAGCC
    CAGCTTGTTG GCCGCCAAAC CCTTGCTACC CCTGCAATGG GTGATATACA GATAGGAATG
    GAGGATAAAA AGGGCCAGTT AGAAGTTGAA GTGATTCGAG CACGAAGCCT CACGCAGAAA
    CCCGGCTCCA AATCCACACC TGCTCCTTAT GTCAAAGTGT ATCTTTTGGA GAATGGGGCC
    TGCATAGCCA AGAAGAAGAC AAGAATTGCA CGAAAGACCC TTGACCCTTT GTACCAACAG
    TCCCTGGTTT TTGATGAAAG TCCACAGGGT AAAGTCCTTC AGGTGATTGT CTGGGGGGAC
    TACGGCAGAA TGGACCACAA ATGCTTCATG GGGGTGGCTC AGATCTTGTT GGAGGAACTC
    GATCTTTCCA GCATGGTCAT CGGCTGGTAC AAGCTGTTCC CACCGTCATC ACTGGTCGAC
    CCCACGCTCA CCCCTCTGAC CCGCCGGGCT TCCCAGTCAT CTCTGGAAAG TTCCACTGGG
    CCCCCCTGCA TCCGGTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237960
    Clone ID Related Accession (Same CDS sequence) XM_024996901.1
    Accession Version XM_024996901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852669.1
    CDS5..3862
    Translation

    Target ORF information:

    RefSeq Version XM_024996901.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGACGCACC GGCAGGCGAG CCCCACCCGG
    TCCCCGCCCA TGGACGCCGC CTTCAGCAGC CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC
    GTGCGAAGCG GCAGCATAGA ACAAGAGTCA GGCCACAAGA AGTTGAAAAG CACCATCCAG
    AGAAGCACTG AGACAGGAAT GGCAGCTGAG ATGAGGAAGA TGGTGAGGCA GCCAAGCCGG
    GAGTCCACGG ACGGCAGCAT CAACAGCTAC AGCTCCGAGG GAAATTTAAT ATTTCCTGGA
    GTGAGACTAG GCGCAGATAG TCAATTCAGT GATTTTCTTG ATGGATTGGG ACCAGCCCAG
    CTTGTTGGCC GCCAAACCCT TGCTACCCCT GCAATGGGTG ATATACAGAT AGGAATGGAG
    GATAAAAAGG GCCAGTTAGA AGTTGAAGTG ATTCGAGCAC GAAGCCTCAC GCAGAAACCC
    GGCTCCAAAT CCACACCTGC TCCTTATGTC AAAGTGTATC TTTTGGAGAA TGGGGCCTGC
    ATAGCCAAGA AGAAGACAAG AATTGCACGA AAGACCCTTG ACCCTTTGTA CCAACAGTCC
    CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTCCTTCAGG TGATTGTCTG GGGGGACTAC
    GGCAGAATGG ACCACAAATG CTTCATGGGG GTGGCTCAGA TCTTGTTGGA GGAACTCGAT
    CTTTCCAGCA TGGTCATCGG CTGGTACAAG CTGTTCCCAC CGTCATCACT GGTCGACCCC
    ACGCTCACCC CTCTGACCCG CCGGGCTTCC CAGTCATCTC TGGAAAGTTC CACTGGGCCC
    CCCTGCATCC GGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237961
    Clone ID Related Accession (Same CDS sequence) XM_024996902.1
    Accession Version XM_024996902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGGCA GACGGATGGG
    GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852670.1
    CDS1125..4970
    Translation

    Target ORF information:

    RefSeq Version XM_024996902.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGGCA GACGGATGGG
    GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237962
    Clone ID Related Accession (Same CDS sequence) XM_024996903.1
    Accession Version XM_024996903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGAGT CAGGCCACAA GAAGTTGAAA AGCACCATCC AGAGAAGCAC TGAGACAGGA
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852671.1
    CDS5..3784
    Translation

    Target ORF information:

    RefSeq Version XM_024996903.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGGCACTCCA GAAAGTCTGA AAGATCTAGC ATCCAAAGCC AGACTAGGAA AGGCACTGCC
    TCTGATGCAG AAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGAGT CAGGCCACAA GAAGTTGAAA AGCACCATCC AGAGAAGCAC TGAGACAGGA
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237963
    Clone ID Related Accession (Same CDS sequence) XM_024996904.1
    Accession Version XM_024996904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852672.1
    CDS5..3781
    Translation

    Target ORF information:

    RefSeq Version XM_024996904.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237968
    Clone ID Related Accession (Same CDS sequence) XM_024996912.1
    Accession Version XM_024996912.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X41
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852680.1
    CDS6..4427
    Translation

    Target ORF information:

    RefSeq Version XM_024996912.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG ACGGATGGGG ACTTCCGGAA GAGCCATCAC CAAGAGCACA
    AGCATGAGCG GAGAGATGTA CACACTGGAG CATAATGATG GCAGCCAGTC GGACACAGCT
    GTGGGCACCG TTGGAGCAGG TGGGAAGAAG AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT
    GCCATAGTGT CTCGAAGGAG CAGAAGCACA TCCCAGCTGA GCCAAACAGA GTCAGGCCAC
    AAGAAGTTGA AAAGCACCAT CCAGAGAAGC ACTGAGACAG GAATGGCAGC TGAGATGAGG
    AAGATGGTGA GGCAGCCAAG CCGGGAGTCC ACGGACGGCA GCATCAACAG CTACAGCTCC
    GAGGGAAATT TAATATTTCC TGGAGTGAGA CTAGGCGCAG ATAGTCAATT CAGTGATTTT
    CTTGATGGAT TGGGACCAGC CCAGCTTGTT GGCCGCCAAA CCCTTGCTAC CCCTGCAATG
    GGTGATATAC AGATAGGAAT GGAGGATAAA AAGGGCCAGT TAGAAGTTGA AGTGATTCGA
    GCACGAAGCC TCACGCAGAA ACCCGGCTCC AAATCCACAC CTGCTCCTTA TGTCAAAGTG
    TATCTTTTGG AGAATGGGGC CTGCATAGCC AAGAAGAAGA CAAGAATTGC ACGAAAGACC
    CTTGACCCTT TGTACCAACA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTCCTT
    CAGGTGATTG TCTGGGGGGA CTACGGCAGA ATGGACCACA AATGCTTCAT GGGGGTGGCT
    CAGATCTTGT TGGAGGAACT CGATCTTTCC AGCATGGTCA TCGGCTGGTA CAAGCTGTTC
    CCACCGTCAT CACTGGTCGA CCCCACGCTC ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA
    TCTCTGGAAA GTTCCACTGG GCCCCCCTGC ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91426
    Clone ID Related Accession (Same CDS sequence) XM_015472748.1 , XM_015464724.1
    Accession Version XM_015472748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328234.1
    CDS144..3206
    Translation

    Target ORF information:

    RefSeq Version XM_015472748.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91426
    Clone ID Related Accession (Same CDS sequence) XM_015472748.1 , XM_015464724.1
    Accession Version XM_015464724.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320210.1
    CDS144..3206
    Translation

    Target ORF information:

    RefSeq Version XM_015464724.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464724.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGTTCATT TACCCCTAAA ATGCAAGGCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91432
    Clone ID Related Accession (Same CDS sequence) XM_015472765.1 , XM_015464741.1
    Accession Version XM_015472765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328251.1
    CDS144..3179
    Translation

    Target ORF information:

    RefSeq Version XM_015472765.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91432
    Clone ID Related Accession (Same CDS sequence) XM_015472765.1 , XM_015464741.1
    Accession Version XM_015464741.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320227.1
    CDS144..3179
    Translation

    Target ORF information:

    RefSeq Version XM_015464741.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464741.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGG TTATAGGTCT
    AGCACTCGAG AAAGCAAACC TTCGACATTA ACCGTGCCAG AGCAGCAGAG AACAACTCAT
    CACCGCTCAC GGTCGGTGTC TCCTCATCGC GGCGACGATC AGGGGAGGCC ACGGTCACGT
    TTACCAAATG TGCCATTACA GAGGAGTTTA GATGAAATTC ATCCAACAAG AAGATCACGT
    TCTCCAACCA GACACCGTGA TGCCTCCCGA AGCCCAATTG ATCAGAGAAT CAGAGATGTG
    GACAGTCAGT GTTTATCAGA ACAAGACAGT GAGCTTCTCA TGCTTCCCAG AGCAAAACGA
    GGACGAAGTG CAGAATGCCT ACACACCACC AGTGACCTGC AGCCCTCCCT TGACAGGGCG
    AGGAGTGCTA GTACCAACTG CTTGAGACCA GATGCTAGTT TGCATTCACC AGAACGAGAA
    AGGGGTAGAT GGTCCCCCTC CCTAGATAGG AGACGACCTC CTAGCCCCAG GATTCAAATC
    CAGCATGCAT CTCCGGAGAA TGACAGGCAC TCCAGAAAGT CTGAAAGATC TAGCATCCAA
    AGCCAGACTA GGAAAGGCAC TGCCTCTGAT GCAGAAAGGG TTCTCCCACC ATGTCTTTCT
    AGAAGGGGAT ACGCAGCCGC GAGAGCGACT GATCAGCCAG TCCTTAGGGG GAAACATCCC
    GCTCGCTCCC GCTCGAGCGA GCACCCTAGT GTCAGAACAC TCTGCTCTAT GCACCACCTT
    GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG CTCCTGACAA GGACGCACCG GCAGGCGAGC
    CCCACCCGGT CCCCGCCCAT GGACGCCGCC TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG
    CAGGTGCCCG TGCGAAGCGG CAGCATAGAA CAAGCAAGCT TAGTAGTGGA GGAGCGAACG
    AGACAGATGA AAATGAAAGT GCATCGATTT AAGCAGACAA CAGGGTCTGG TTCTAGTCAA
    GAACTTGATC GCGAGCAATA TTCCAAGTAT AACGCACACA CAGATCAGTA CAGAAGCTGC
    GACAACGTCT CCACCAGATC CTCAGACAGC GACGTCAGCG ACGCGTCTGC CGTGTCCCGA
    ACCAGCAGTG CCTCCCGCCT CAGCAGCACC AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT
    CGGGGCAGGA TCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22452
    Clone ID Related Accession (Same CDS sequence) XM_015464725.1 , XM_015472749.1
    Accession Version XM_015464725.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320211.1
    CDS144..3182
    Translation

    Target ORF information:

    RefSeq Version XM_015464725.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22452
    Clone ID Related Accession (Same CDS sequence) XM_015464725.1 , XM_015472749.1
    Accession Version XM_015472749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328235.1
    CDS144..3182
    Translation

    Target ORF information:

    RefSeq Version XM_015472749.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGACG GATGGGGACT TCCGGAAGAG CCATCACCAA GAGCACAAGC
    ATGAGCGGAG AGATGTACAC ACTGGAGCAT AATGATGGCA GCCAGTCGGA CACAGCTGTG
    GGCACCGTTG GAGCAGGTGG GAAGAAGAGG AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC
    ATAGTGTCTC GAAGGAGCAG AAGCACATCC CAGCTGAGCC AAACAGAGTC AGGCCACAAG
    AAGTTGAAAA GCACCATCCA GAGAAGCACT GAGACAGGAA TGGCAGCTGA GATGAGGAAG
    ATGGTGAGGC AGCCAAGCCG GGAGTCCACG GACGGCAGCA TCAACAGCTA CAGCTCCGAG
    GGAAATTTAA TATTTCCTGG AGTGAGACTA GGCGCAGATA GTCAATTCAG TGATTTTCTT
    GATGGATTGG GACCAGCCCA GCTTGTTGGC CGCCAAACCC TTGCTACCCC TGCAATGGGT
    GATATACAGA TAGGAATGGA GGATAAAAAG GGCCAGTTAG AAGTTGAAGT GATTCGAGCA
    CGAAGCCTCA CGCAGAAACC CGGCTCCAAA TCCACACCTG CTCCTTATGT CAAAGTGTAT
    CTTTTGGAGA ATGGGGCCTG CATAGCCAAG AAGAAGACAA GAATTGCACG AAAGACCCTT
    GACCCTTTGT ACCAACAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTCCTTCAG
    GTGATTGTCT GGGGGGACTA CGGCAGAATG GACCACAAAT GCTTCATGGG GGTGGCTCAG
    ATCTTGTTGG AGGAACTCGA TCTTTCCAGC ATGGTCATCG GCTGGTACAA GCTGTTCCCA
    CCGTCATCAC TGGTCGACCC CACGCTCACC CCTCTGACCC GCCGGGCTTC CCAGTCATCT
    CTGGAAAGTT CCACTGGGCC CCCCTGCATC CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52634
    Clone ID Related Accession (Same CDS sequence) XM_015464723.1 , XM_015472747.1
    Accession Version XM_015464723.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320209.1
    CDS144..3209
    Translation

    Target ORF information:

    RefSeq Version XM_015464723.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464723.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52634
    Clone ID Related Accession (Same CDS sequence) XM_015464723.1 , XM_015472747.1
    Accession Version XM_015472747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328233.1
    CDS144..3209
    Translation

    Target ORF information:

    RefSeq Version XM_015472747.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGTGACC TGCAGCCCTC CCTTGACAGG
    GCGAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATGCTA GTTTGCATTC ACCAGAACGA
    GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGACGAC CTCCTAGCCC CAGGATTCAA
    ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATCTAGCATC
    CAAAGCCAGA CTAGGAAAGG CACTGCCTCT GATGCAGAAA GGGTTCTCCC ACCATGTCTT
    TCTAGAAGGG GATACGCAGC CGCGAGAGCG ACTGATCAGC CAGTCCTTAG GGGGAAACAT
    CCCGCTCGCT CCCGCTCGAG CGAGCACCCT AGTGTCAGAA CACTCTGCTC TATGCACCAC
    CTTGCCCCCG GAGGGTCCGC GCCACCTTCT CCGCTCCTGA CAAGGACGCA CCGGCAGGCG
    AGCCCCACCC GGTCCCCGCC CATGGACGCC GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC
    CCGCAGGTGC CCGTGCGAAG CGGCAGCATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA
    ACGAGACAGA TGAAAATGAA AGTGCATCGA TTTAAGCAGA CAACAGGGTC TGGTTCTAGT
    CAAGAACTTG ATCGCGAGCA ATATTCCAAG TATAACGCAC ACACAGATCA GTACAGAAGC
    TGCGACAACG TCTCCACCAG ATCCTCAGAC AGCGACGTCA GCGACGCGTC TGCCGTGTCC
    CGAACCAGCA GTGCCTCCCG CCTCAGCAGC ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG
    CCTCGGGGCA GGATCAGTTC ATTTACCCCT AAAATGCAAG GCAGACGGAT GGGGACTTCC
    GGAAGAGCCA TCACCAAGAG CACAAGCATG AGCGGAGAGA TGTACACACT GGAGCATAAT
    GATGGCAGCC AGTCGGACAC AGCTGTGGGC ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG
    TCCAGCCTGA GTGCCAAGGT GGTTGCCATA GTGTCTCGAA GGAGCAGAAG CACATCCCAG
    CTGAGCCAAA CAGAGTCAGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG AAGCACTGAG
    ACAGGAATGG CAGCTGAGAT GAGGAAGATG GTGAGGCAGC CAAGCCGGGA GTCCACGGAC
    GGCAGCATCA ACAGCTACAG CTCCGAGGGA AATTTAATAT TTCCTGGAGT GAGACTAGGC
    GCAGATAGTC AATTCAGTGA TTTTCTTGAT GGATTGGGAC CAGCCCAGCT TGTTGGCCGC
    CAAACCCTTG CTACCCCTGC AATGGGTGAT ATACAGATAG GAATGGAGGA TAAAAAGGGC
    CAGTTAGAAG TTGAAGTGAT TCGAGCACGA AGCCTCACGC AGAAACCCGG CTCCAAATCC
    ACACCTGCTC CTTATGTCAA AGTGTATCTT TTGGAGAATG GGGCCTGCAT AGCCAAGAAG
    AAGACAAGAA TTGCACGAAA GACCCTTGAC CCTTTGTACC AACAGTCCCT GGTTTTTGAT
    GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG ATTGTCTGGG GGGACTACGG CAGAATGGAC
    CACAAATGCT TCATGGGGGT GGCTCAGATC TTGTTGGAGG AACTCGATCT TTCCAGCATG
    GTCATCGGCT GGTACAAGCT GTTCCCACCG TCATCACTGG TCGACCCCAC GCTCACCCCT
    CTGACCCGCC GGGCTTCCCA GTCATCTCTG GAAAGTTCCA CTGGGCCCCC CTGCATCCGG
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52643
    Clone ID Related Accession (Same CDS sequence) XM_015464740.1 , XM_015472764.1
    Accession Version XM_015464740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645519.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGACATC TTGTTAGGCA CTATAAAACA
    TTACCGCCTA AGATGCCCTT ATTAGAGACC TGTTCTCACT GGGATATTTA CAGCTCAATT
    CTGCCTGCAC GTACCAAAAC CCAAACCCTT CACCATGAAT GTTTTCACTC GAGAGCACTG
    AGGTTTACTG ATGAGATTCT GGTTGGTGAC CTGCAGCCCT CCCTTGACAG GGCGAGGAGT
    GCTAGTACCA ACTGCTTGAG ACCAGATGCT AGTTTGCATT CACCAGAACG AGAAAGGGGT
    AGATGGTCCC CCTCCCTAGA TAGGAGACGA CCTCCTAGCC CCAGGATTCA AATCCAGCAT
    GCATCTCCGG AGAATGACAG GCACTCCAGA AAGTCTGAAA GATCTAGCAT CCAAAGCCAG
    ACTAGGAAAG GCACTGCCTC TGATGCAGAA AGGGTTCTCC CACCATGTCT TTCTAGAAGG
    GGATACGCAG CCGCGAGAGC GACTGATCAG CCAGTCCTTA GGGGGAAACA TCCCGCTCGC
    TCCCGCTCGA GCGAGCACCC TAGTGTCAGA ACACTCTGCT CTATGCACCA CCTTGCCCCC
    GGAGGGTCCG CGCCACCTTC TCCGCTCCTG ACAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320226.1
    CDS144..3380
    Translation

    Target ORF information:

    RefSeq Version XM_015464740.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGACATC TTGTTAGGCA CTATAAAACA
    TTACCGCCTA AGATGCCCTT ATTAGAGACC TGTTCTCACT GGGATATTTA CAGCTCAATT
    CTGCCTGCAC GTACCAAAAC CCAAACCCTT CACCATGAAT GTTTTCACTC GAGAGCACTG
    AGGTTTACTG ATGAGATTCT GGTTGGTGAC CTGCAGCCCT CCCTTGACAG GGCGAGGAGT
    GCTAGTACCA ACTGCTTGAG ACCAGATGCT AGTTTGCATT CACCAGAACG AGAAAGGGGT
    AGATGGTCCC CCTCCCTAGA TAGGAGACGA CCTCCTAGCC CCAGGATTCA AATCCAGCAT
    GCATCTCCGG AGAATGACAG GCACTCCAGA AAGTCTGAAA GATCTAGCAT CCAAAGCCAG
    ACTAGGAAAG GCACTGCCTC TGATGCAGAA AGGGTTCTCC CACCATGTCT TTCTAGAAGG
    GGATACGCAG CCGCGAGAGC GACTGATCAG CCAGTCCTTA GGGGGAAACA TCCCGCTCGC
    TCCCGCTCGA GCGAGCACCC TAGTGTCAGA ACACTCTGCT CTATGCACCA CCTTGCCCCC
    GGAGGGTCCG CGCCACCTTC TCCGCTCCTG ACAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52643
    Clone ID Related Accession (Same CDS sequence) XM_015464740.1 , XM_015472764.1
    Accession Version XM_015472764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGACATC TTGTTAGGCA CTATAAAACA
    TTACCGCCTA AGATGCCCTT ATTAGAGACC TGTTCTCACT GGGATATTTA CAGCTCAATT
    CTGCCTGCAC GTACCAAAAC CCAAACCCTT CACCATGAAT GTTTTCACTC GAGAGCACTG
    AGGTTTACTG ATGAGATTCT GGTTGGTGAC CTGCAGCCCT CCCTTGACAG GGCGAGGAGT
    GCTAGTACCA ACTGCTTGAG ACCAGATGCT AGTTTGCATT CACCAGAACG AGAAAGGGGT
    AGATGGTCCC CCTCCCTAGA TAGGAGACGA CCTCCTAGCC CCAGGATTCA AATCCAGCAT
    GCATCTCCGG AGAATGACAG GCACTCCAGA AAGTCTGAAA GATCTAGCAT CCAAAGCCAG
    ACTAGGAAAG GCACTGCCTC TGATGCAGAA AGGGTTCTCC CACCATGTCT TTCTAGAAGG
    GGATACGCAG CCGCGAGAGC GACTGATCAG CCAGTCCTTA GGGGGAAACA TCCCGCTCGC
    TCCCGCTCGA GCGAGCACCC TAGTGTCAGA ACACTCTGCT CTATGCACCA CCTTGCCCCC
    GGAGGGTCCG CGCCACCTTC TCCGCTCCTG ACAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328250.1
    CDS144..3380
    Translation

    Target ORF information:

    RefSeq Version XM_015472764.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCCAAAG AATCAGGTGC ATTACTGGGT CTGAAAGTTG TTGGAGGAAA AATGACTGAC 
    TTAGGACGCC TCGGTGCCTT CATCACCAAA GTAAAGAAGG GTAGCCTTGC AGATGTGGTT
    GGACACTTGA GAGCAGGGGA TGAAGTTCTA GAATGGAATG GTAAACCCCT GCCGGGAGCT
    ACAAATGAAG AAGTTTACAA CATTATTTTA GAATCAAAAT CAGAACCTCA AGTTGAAATT
    ATTGTTTCAA GGCCTATTGG TGACATTCCA AGGATTCCTG AGAGCTCCCA TCCACCACTG
    GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG AAGATGGAAA GGCCTTCCAT TTCTGTAATT
    TCTCCCACAA GCCCTGGAGC TCTCAAAGAT GCCCCACAAG TATTACCAGG ACAACTTTCT
    GTGAAGCTGT GGTATGACAA GGTGGGCCAC CAACTGATTG TCAATGTTCT GCAAGCAACA
    GACCTACCCC CTAGAGTAGA TGGGCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT
    CCAGATAGAA GTGATAAAAG TAAAAGGAGA ACCAAAACGG TAAAGAAAGT CCTCGAACCA
    AAGTGGAATC AGACTTTTGT CTATTCGCAC GTGCATCGTA GAGACTTTAG AGAACGGATG
    TTGGAAATAA CCGTGTGGGA CCAGCCCAGG GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA
    GAGATCCTCA TAGAGTTGGA AACAGCGCTT CTAGACGATG AGCCACATTG GTATAAACTT
    CAGACTCACG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA
    CACACCCACG GCGAAAGCGC CAGCAAGAAG CTGCAAAGGT CCCAGCGAAT CAGTGACAGT
    GACATGTCAG ACTATGAGGT TGATGATGCG ATTGGAGTGG TCCCTCCAGT AGGTTATAGG
    TCTAGCACTC GAGAAAGCAA ACCTTCGACA TTAACCGTGC CAGAGCAGCA GAGAACAACT
    CATCACCGCT CACGGTCGGT GTCTCCTCAT CGCGGCGACG ATCAGGGGAG GCCACGGTCA
    CGTTTACCAA ATGTGCCATT ACAGAGGAGT TTAGATGAAA TTCATCCAAC AAGAAGATCA
    CGTTCTCCAA CCAGACACCG TGATGCCTCC CGAAGCCCAA TTGATCAGAG AATCAGAGAT
    GTGGACAGTC AGTGTTTATC AGAACAAGAC AGTGAGCTTC TCATGCTTCC CAGAGCAAAA
    CGAGGACGAA GTGCAGAATG CCTACACACC ACCAGACATC TTGTTAGGCA CTATAAAACA
    TTACCGCCTA AGATGCCCTT ATTAGAGACC TGTTCTCACT GGGATATTTA CAGCTCAATT
    CTGCCTGCAC GTACCAAAAC CCAAACCCTT CACCATGAAT GTTTTCACTC GAGAGCACTG
    AGGTTTACTG ATGAGATTCT GGTTGGTGAC CTGCAGCCCT CCCTTGACAG GGCGAGGAGT
    GCTAGTACCA ACTGCTTGAG ACCAGATGCT AGTTTGCATT CACCAGAACG AGAAAGGGGT
    AGATGGTCCC CCTCCCTAGA TAGGAGACGA CCTCCTAGCC CCAGGATTCA AATCCAGCAT
    GCATCTCCGG AGAATGACAG GCACTCCAGA AAGTCTGAAA GATCTAGCAT CCAAAGCCAG
    ACTAGGAAAG GCACTGCCTC TGATGCAGAA AGGGTTCTCC CACCATGTCT TTCTAGAAGG
    GGATACGCAG CCGCGAGAGC GACTGATCAG CCAGTCCTTA GGGGGAAACA TCCCGCTCGC
    TCCCGCTCGA GCGAGCACCC TAGTGTCAGA ACACTCTGCT CTATGCACCA CCTTGCCCCC
    GGAGGGTCCG CGCCACCTTC TCCGCTCCTG ACAAGGACGC ACCGGCAGGC GAGCCCCACC
    CGGTCCCCGC CCATGGACGC CGCCTTCAGC AGCCGCAGGG GCAGGCAGCT CCCGCAGGTG
    CCCGTGCGAA GCGGCAGCAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG
    ATGAAAATGA AAGTGCATCG ATTTAAGCAG ACAACAGGGT CTGGTTCTAG TCAAGAACTT
    GATCGCGAGC AATATTCCAA GTATAACGCA CACACAGATC AGTACAGAAG CTGCGACAAC
    GTCTCCACCA GATCCTCAGA CAGCGACGTC AGCGACGCGT CTGCCGTGTC CCGAACCAGC
    AGTGCCTCCC GCCTCAGCAG CACCAGCTTC CTGTCCGAGC AGTCTGAGCG GCCTCGGGGC
    AGGATCAGTT CATTTACCCC TAAAATGCAA GGCAGACGGA TGGGGACTTC CGGAAGAGCC
    ATCACCAAGA GCACAAGCAT GAGCGGAGAG ATGTACACAC TGGAGCATAA TGATGGCAGC
    CAGTCGGACA CAGCTGTGGG CACCGTTGGA GCAGGTGGGA AGAAGAGGAG GTCCAGCCTG
    AGTGCCAAGG TGGTTGCCAT AGTGTCTCGA AGGAGCAGAA GCACATCCCA GCTGAGCCAA
    ACAGAGTCAG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GAAGCACTGA GACAGGAATG
    GCAGCTGAGA TGAGGAAGAT GGTGAGGCAG CCAAGCCGGG AGTCCACGGA CGGCAGCATC
    AACAGCTACA GCTCCGAGGG AAATTTAATA TTTCCTGGAG TGAGACTAGG CGCAGATAGT
    CAATTCAGTG ATTTTCTTGA TGGATTGGGA CCAGCCCAGC TTGTTGGCCG CCAAACCCTT
    GCTACCCCTG CAATGGGTGA TATACAGATA GGAATGGAGG ATAAAAAGGG CCAGTTAGAA
    GTTGAAGTGA TTCGAGCACG AAGCCTCACG CAGAAACCCG GCTCCAAATC CACACCTGCT
    CCTTATGTCA AAGTGTATCT TTTGGAGAAT GGGGCCTGCA TAGCCAAGAA GAAGACAAGA
    ATTGCACGAA AGACCCTTGA CCCTTTGTAC CAACAGTCCC TGGTTTTTGA TGAAAGTCCA
    CAGGGTAAAG TCCTTCAGGT GATTGTCTGG GGGGACTACG GCAGAATGGA CCACAAATGC
    TTCATGGGGG TGGCTCAGAT CTTGTTGGAG GAACTCGATC TTTCCAGCAT GGTCATCGGC
    TGGTACAAGC TGTTCCCACC GTCATCACTG GTCGACCCCA CGCTCACCCC TCTGACCCGC
    CGGGCTTCCC AGTCATCTCT GGAAAGTTCC ACTGGGCCCC CCTGCATCCG GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237933
    Clone ID Related Accession (Same CDS sequence) XM_015472747.2
    Accession Version XM_015472747.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328233.2
    CDS6..4721
    Translation

    Target ORF information:

    RefSeq Version XM_015472747.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472747.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGTTC ATTTACCCCT
    AAAATGCAAG GCAGACGGAT GGGGACTTCC GGAAGAGCCA TCACCAAGAG CACAAGCATG
    AGCGGAGAGA TGTACACACT GGAGCATAAT GATGGCAGCC AGTCGGACAC AGCTGTGGGC
    ACCGTTGGAG CAGGTGGGAA GAAGAGGAGG TCCAGCCTGA GTGCCAAGGT GGTTGCCATA
    GTGTCTCGAA GGAGCAGAAG CACATCCCAG CTGAGCCAAA CAGAGTCAGG CCACAAGAAG
    TTGAAAAGCA CCATCCAGAG AAGCACTGAG ACAGGAATGG CAGCTGAGAT GAGGAAGATG
    GTGAGGCAGC CAAGCCGGGA GTCCACGGAC GGCAGCATCA ACAGCTACAG CTCCGAGGGA
    AATTTAATAT TTCCTGGAGT GAGACTAGGC GCAGATAGTC AATTCAGTGA TTTTCTTGAT
    GGATTGGGAC CAGCCCAGCT TGTTGGCCGC CAAACCCTTG CTACCCCTGC AATGGGTGAT
    ATACAGATAG GAATGGAGGA TAAAAAGGGC CAGTTAGAAG TTGAAGTGAT TCGAGCACGA
    AGCCTCACGC AGAAACCCGG CTCCAAATCC ACACCTGCTC CTTATGTCAA AGTGTATCTT
    TTGGAGAATG GGGCCTGCAT AGCCAAGAAG AAGACAAGAA TTGCACGAAA GACCCTTGAC
    CCTTTGTACC AACAGTCCCT GGTTTTTGAT GAAAGTCCAC AGGGTAAAGT CCTTCAGGTG
    ATTGTCTGGG GGGACTACGG CAGAATGGAC CACAAATGCT TCATGGGGGT GGCTCAGATC
    TTGTTGGAGG AACTCGATCT TTCCAGCATG GTCATCGGCT GGTACAAGCT GTTCCCACCG
    TCATCACTGG TCGACCCCAC GCTCACCCCT CTGACCCGCC GGGCTTCCCA GTCATCTCTG
    GAAAGTTCCA CTGGGCCCCC CTGCATCCGG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237934
    Clone ID Related Accession (Same CDS sequence) XM_015472748.2
    Accession Version XM_015472748.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGGGTAGAT GGTCCCCCTC CCTAGATAGG
    AGACGACCTC CTAGCCCCAG GATTCAAATC CAGCATGCAT CTCCGGAGAA TGACAGGCAC
    TCCAGAAAGT CTGAAAGATC TAGCATCCAA AGCCAGACTA GGAAAGGCAC TGCCTCTGAT
    GCAGAAAGGG TTCTCCCACC ATGTCTTTCT AGAAGGGGAT ACGCAGCCGC GAGAGCGACT
    GATCAGCCAG TCCTTAGGGG GAAACATCCC GCTCGCTCCC GCTCGAGCGA GCACCCTAGT
    GTCAGAACAC TCTGCTCTAT GCACCACCTT GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG
    CTCCTGACAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGCAAGCT TAGTAGTGGA GGAGCGAACG AGACAGATGA AAATGAAAGT GCATCGATTT
    AAGCAGACAA CAGGGTCTGG TTCTAGTCAA GAACTTGATC GCGAGCAATA TTCCAAGTAT
    AACGCACACA CAGATCAGTA CAGAAGCTGC GACAACGTCT CCACCAGATC CTCAGACAGC
    GACGTCAGCG ACGCGTCTGC CGTGTCCCGA ACCAGCAGTG CCTCCCGCCT CAGCAGCACC
    AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT CGGGGCAGGA TCAGTTCATT TACCCCTAAA
    ATGCAAGGCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328234.2
    CDS7..4719
    Translation

    Target ORF information:

    RefSeq Version XM_015472748.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472748.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGGGTAGAT GGTCCCCCTC CCTAGATAGG
    AGACGACCTC CTAGCCCCAG GATTCAAATC CAGCATGCAT CTCCGGAGAA TGACAGGCAC
    TCCAGAAAGT CTGAAAGATC TAGCATCCAA AGCCAGACTA GGAAAGGCAC TGCCTCTGAT
    GCAGAAAGGG TTCTCCCACC ATGTCTTTCT AGAAGGGGAT ACGCAGCCGC GAGAGCGACT
    GATCAGCCAG TCCTTAGGGG GAAACATCCC GCTCGCTCCC GCTCGAGCGA GCACCCTAGT
    GTCAGAACAC TCTGCTCTAT GCACCACCTT GCCCCCGGAG GGTCCGCGCC ACCTTCTCCG
    CTCCTGACAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGCAAGCT TAGTAGTGGA GGAGCGAACG AGACAGATGA AAATGAAAGT GCATCGATTT
    AAGCAGACAA CAGGGTCTGG TTCTAGTCAA GAACTTGATC GCGAGCAATA TTCCAAGTAT
    AACGCACACA CAGATCAGTA CAGAAGCTGC GACAACGTCT CCACCAGATC CTCAGACAGC
    GACGTCAGCG ACGCGTCTGC CGTGTCCCGA ACCAGCAGTG CCTCCCGCCT CAGCAGCACC
    AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT CGGGGCAGGA TCAGTTCATT TACCCCTAAA
    ATGCAAGGCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237935
    Clone ID Related Accession (Same CDS sequence) XM_015472749.2
    Accession Version XM_015472749.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328235.2
    CDS7..4695
    Translation

    Target ORF information:

    RefSeq Version XM_015472749.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472749.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGGTTCTCCC ACCATGTCTT TCTAGAAGGG GATACGCAGC CGCGAGAGCG
    ACTGATCAGC CAGTCCTTAG GGGGAAACAT CCCGCTCGCT CCCGCTCGAG CGAGCACCCT
    AGTGTCAGAA CACTCTGCTC TATGCACCAC CTTGCCCCCG GAGGGTCCGC GCCACCTTCT
    CCGCTCCTGA CAAGGACGCA CCGGCAGGCG AGCCCCACCC GGTCCCCGCC CATGGACGCC
    GCCTTCAGCA GCCGCAGGGG CAGGCAGCTC CCGCAGGTGC CCGTGCGAAG CGGCAGCATA
    GAACAAGCAA GCTTAGTAGT GGAGGAGCGA ACGAGACAGA TGAAAATGAA AGTGCATCGA
    TTTAAGCAGA CAACAGGGTC TGGTTCTAGT CAAGAACTTG ATCGCGAGCA ATATTCCAAG
    TATAACGCAC ACACAGATCA GTACAGAAGC TGCGACAACG TCTCCACCAG ATCCTCAGAC
    AGCGACGTCA GCGACGCGTC TGCCGTGTCC CGAACCAGCA GTGCCTCCCG CCTCAGCAGC
    ACCAGCTTCC TGTCCGAGCA GTCTGAGCGG CCTCGGGGCA GGATCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237936
    Clone ID Related Accession (Same CDS sequence) XM_015472750.2
    Accession Version XM_015472750.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAATAG GTTATAGGTC TAGCACTCGA GAAAGCAAAC CTTCGACATT AACCGTGCCA
    GAGCAGCAGA GAACAACTCA TCACCGCTCA CGGTCGGTGT CTCCTCATCG CGGCGACGAT
    CAGGGGAGGC CACGGTCACG TTTACCAAAT GTGCCATTAC AGAGGAGTTT AGATGAAATT
    CATCCAACAA GAAGATCACG TTCTCCAACC AGACACCGTG ATGCCTCCCG AAGCCCAATT
    GATCAGAGAA TCAGAGATGT GGACAGTCAG TGTTTATCAG AACAAGACAG TGAGCTTCTC
    ATGCTTCCCA GAGCAAAACG AGGACGAAGT GCAGAATGCC TACACACCAC CAGTGACCTG
    CAGCCCTCCC TTGACAGGGC GAGGAGTGCT AGTACCAACT GCTTGAGACC AGATGCTAGT
    TTGCATTCAC CAGAACGAGA AAGGGGTAGA TGGTCCCCCT CCCTAGATAG GAGACGACCT
    CCTAGCCCCA GGATTCAAAT CCAGCATGCA TCTCCGGAGA ATGACAGGCA CTCCAGAAAG
    TCTGAAAGAT CTAGCATCCA AAGCCAGACT AGGAAAGGCA CTGCCTCTGA TGCAGAAAGG
    GTTCTCCCAC CATGTCTTTC TAGAAGGGGA TACGCAGCCG CGAGAGCGAC TGATCAGCCA
    GTCCTTAGGG GGAAACATCC CGCTCGCTCC CGCTCGAGCG AGCACCCTAG TGTCAGAACA
    CTCTGCTCTA TGCACCACCT TGCCCCCGGA GGGTCCGCGC CACCTTCTCC GCTCCTGACA
    AGGACGCACC GGCAGGCGAG CCCCACCCGG TCCCCGCCCA TGGACGCCGC CTTCAGCAGC
    CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC GTGCGAAGCG GCAGCATAGA ACAAGCAAGC
    TTAGTAGTGG AGGAGCGAAC GAGACAGATG AAAATGAAAG TGCATCGATT TAAGCAGACA
    ACAGGGTCTG GTTCTAGTCA AGAACTTGAT CGCGAGCAAT ATTCCAAGTA TAACGCACAC
    ACAGATCAGT ACAGAAGCTG CGACAACGTC TCCACCAGAT CCTCAGACAG CGACGTCAGC
    GACGCGTCTG CCGTGTCCCG AACCAGCAGT GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG
    TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG ATCAGTTCAT TTACCCCTAA AATGCAAGGC
    AGACGGATGG GGACTTCCGG AAGAGCCATC ACCAAGAGCA CAAGCATGAG CGGAGAGATG
    TACACACTGG AGCATAATGA TGGCAGCCAG TCGGACACAG CTGTGGGCAC CGTTGGAGCA
    GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT GCCAAGGTGG TTGCCATAGT GTCTCGAAGG
    AGCAGAAGCA CATCCCAGCT GAGCCAAACA GAGTCAGGCC ACAAGAAGTT GAAAAGCACC
    ATCCAGAGAA GCACTGAGAC AGGAATGGCA GCTGAGATGA GGAAGATGGT GAGGCAGCCA
    AGCCGGGAGT CCACGGACGG CAGCATCAAC AGCTACAGCT CCGAGGGAAA TTTAATATTT
    CCTGGAGTGA GACTAGGCGC AGATAGTCAA TTCAGTGATT TTCTTGATGG ATTGGGACCA
    GCCCAGCTTG TTGGCCGCCA AACCCTTGCT ACCCCTGCAA TGGGTGATAT ACAGATAGGA
    ATGGAGGATA AAAAGGGCCA GTTAGAAGTT GAAGTGATTC GAGCACGAAG CCTCACGCAG
    AAACCCGGCT CCAAATCCAC ACCTGCTCCT TATGTCAAAG TGTATCTTTT GGAGAATGGG
    GCCTGCATAG CCAAGAAGAA GACAAGAATT GCACGAAAGA CCCTTGACCC TTTGTACCAA
    CAGTCCCTGG TTTTTGATGA AAGTCCACAG GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG
    GACTACGGCA GAATGGACCA CAAATGCTTC ATGGGGGTGG CTCAGATCTT GTTGGAGGAA
    CTCGATCTTT CCAGCATGGT CATCGGCTGG TACAAGCTGT TCCCACCGTC ATCACTGGTC
    GACCCCACGC TCACCCCTCT GACCCGCCGG GCTTCCCAGT CATCTCTGGA AAGTTCCACT
    GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328236.2
    CDS7..4650
    Translation

    Target ORF information:

    RefSeq Version XM_015472750.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472750.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAATAG GTTATAGGTC TAGCACTCGA GAAAGCAAAC CTTCGACATT AACCGTGCCA
    GAGCAGCAGA GAACAACTCA TCACCGCTCA CGGTCGGTGT CTCCTCATCG CGGCGACGAT
    CAGGGGAGGC CACGGTCACG TTTACCAAAT GTGCCATTAC AGAGGAGTTT AGATGAAATT
    CATCCAACAA GAAGATCACG TTCTCCAACC AGACACCGTG ATGCCTCCCG AAGCCCAATT
    GATCAGAGAA TCAGAGATGT GGACAGTCAG TGTTTATCAG AACAAGACAG TGAGCTTCTC
    ATGCTTCCCA GAGCAAAACG AGGACGAAGT GCAGAATGCC TACACACCAC CAGTGACCTG
    CAGCCCTCCC TTGACAGGGC GAGGAGTGCT AGTACCAACT GCTTGAGACC AGATGCTAGT
    TTGCATTCAC CAGAACGAGA AAGGGGTAGA TGGTCCCCCT CCCTAGATAG GAGACGACCT
    CCTAGCCCCA GGATTCAAAT CCAGCATGCA TCTCCGGAGA ATGACAGGCA CTCCAGAAAG
    TCTGAAAGAT CTAGCATCCA AAGCCAGACT AGGAAAGGCA CTGCCTCTGA TGCAGAAAGG
    GTTCTCCCAC CATGTCTTTC TAGAAGGGGA TACGCAGCCG CGAGAGCGAC TGATCAGCCA
    GTCCTTAGGG GGAAACATCC CGCTCGCTCC CGCTCGAGCG AGCACCCTAG TGTCAGAACA
    CTCTGCTCTA TGCACCACCT TGCCCCCGGA GGGTCCGCGC CACCTTCTCC GCTCCTGACA
    AGGACGCACC GGCAGGCGAG CCCCACCCGG TCCCCGCCCA TGGACGCCGC CTTCAGCAGC
    CGCAGGGGCA GGCAGCTCCC GCAGGTGCCC GTGCGAAGCG GCAGCATAGA ACAAGCAAGC
    TTAGTAGTGG AGGAGCGAAC GAGACAGATG AAAATGAAAG TGCATCGATT TAAGCAGACA
    ACAGGGTCTG GTTCTAGTCA AGAACTTGAT CGCGAGCAAT ATTCCAAGTA TAACGCACAC
    ACAGATCAGT ACAGAAGCTG CGACAACGTC TCCACCAGAT CCTCAGACAG CGACGTCAGC
    GACGCGTCTG CCGTGTCCCG AACCAGCAGT GCCTCCCGCC TCAGCAGCAC CAGCTTCCTG
    TCCGAGCAGT CTGAGCGGCC TCGGGGCAGG ATCAGTTCAT TTACCCCTAA AATGCAAGGC
    AGACGGATGG GGACTTCCGG AAGAGCCATC ACCAAGAGCA CAAGCATGAG CGGAGAGATG
    TACACACTGG AGCATAATGA TGGCAGCCAG TCGGACACAG CTGTGGGCAC CGTTGGAGCA
    GGTGGGAAGA AGAGGAGGTC CAGCCTGAGT GCCAAGGTGG TTGCCATAGT GTCTCGAAGG
    AGCAGAAGCA CATCCCAGCT GAGCCAAACA GAGTCAGGCC ACAAGAAGTT GAAAAGCACC
    ATCCAGAGAA GCACTGAGAC AGGAATGGCA GCTGAGATGA GGAAGATGGT GAGGCAGCCA
    AGCCGGGAGT CCACGGACGG CAGCATCAAC AGCTACAGCT CCGAGGGAAA TTTAATATTT
    CCTGGAGTGA GACTAGGCGC AGATAGTCAA TTCAGTGATT TTCTTGATGG ATTGGGACCA
    GCCCAGCTTG TTGGCCGCCA AACCCTTGCT ACCCCTGCAA TGGGTGATAT ACAGATAGGA
    ATGGAGGATA AAAAGGGCCA GTTAGAAGTT GAAGTGATTC GAGCACGAAG CCTCACGCAG
    AAACCCGGCT CCAAATCCAC ACCTGCTCCT TATGTCAAAG TGTATCTTTT GGAGAATGGG
    GCCTGCATAG CCAAGAAGAA GACAAGAATT GCACGAAAGA CCCTTGACCC TTTGTACCAA
    CAGTCCCTGG TTTTTGATGA AAGTCCACAG GGTAAAGTCC TTCAGGTGAT TGTCTGGGGG
    GACTACGGCA GAATGGACCA CAAATGCTTC ATGGGGGTGG CTCAGATCTT GTTGGAGGAA
    CTCGATCTTT CCAGCATGGT CATCGGCTGG TACAAGCTGT TCCCACCGTC ATCACTGGTC
    GACCCCACGC TCACCCCTCT GACCCGCCGG GCTTCCCAGT CATCTCTGGA AAGTTCCACT
    GGGCCCCCCT GCATCCGGTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237937
    Clone ID Related Accession (Same CDS sequence) XM_024996879.1
    Accession Version XM_024996879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGGT TCTCCCACCA
    TGTCTTTCTA GAAGGGGATA CGCAGCCGCG AGAGCGACTG ATCAGCCAGT CCTTAGGGGG
    AAACATCCCG CTCGCTCCCG CTCGAGCGAG CACCCTAGTG TCAGAACACT CTGCTCTATG
    CACCACCTTG CCCCCGGAGG GTCCGCGCCA CCTTCTCCGC TCCTGACAAG GACGCACCGG
    CAGGCGAGCC CCACCCGGTC CCCGCCCATG GACGCCGCCT TCAGCAGCCG CAGGGGCAGG
    CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC AGCATAGAAC AAGCAAGCTT AGTAGTGGAG
    GAGCGAACGA GACAGATGAA AATGAAAGTG CATCGATTTA AGCAGACAAC AGGGTCTGGT
    TCTAGTCAAG AACTTGATCG CGAGCAATAT TCCAAGTATA ACGCACACAC AGATCAGTAC
    AGAAGCTGCG ACAACGTCTC CACCAGATCC TCAGACAGCG ACGTCAGCGA CGCGTCTGCC
    GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC AGCAGCACCA GCTTCCTGTC CGAGCAGTCT
    GAGCGGCCTC GGGGCAGGAT CAGTTCATTT ACCCCTAAAA TGCAAGGCAG ACGGATGGGG
    ACTTCCGGAA GAGCCATCAC CAAGAGCACA AGCATGAGCG GAGAGATGTA CACACTGGAG
    CATAATGATG GCAGCCAGTC GGACACAGCT GTGGGCACCG TTGGAGCAGG TGGGAAGAAG
    AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT GCCATAGTGT CTCGAAGGAG CAGAAGCACA
    TCCCAGCTGA GCCAAACAGA GTCAGGCCAC AAGAAGTTGA AAAGCACCAT CCAGAGAAGC
    ACTGAGACAG GAATGGCAGC TGAGATGAGG AAGATGGTGA GGCAGCCAAG CCGGGAGTCC
    ACGGACGGCA GCATCAACAG CTACAGCTCC GAGGGAAATT TAATATTTCC TGGAGTGAGA
    CTAGGCGCAG ATAGTCAATT CAGTGATTTT CTTGATGGAT TGGGACCAGC CCAGCTTGTT
    GGCCGCCAAA CCCTTGCTAC CCCTGCAATG GGTGATATAC AGATAGGAAT GGAGGATAAA
    AAGGGCCAGT TAGAAGTTGA AGTGATTCGA GCACGAAGCC TCACGCAGAA ACCCGGCTCC
    AAATCCACAC CTGCTCCTTA TGTCAAAGTG TATCTTTTGG AGAATGGGGC CTGCATAGCC
    AAGAAGAAGA CAAGAATTGC ACGAAAGACC CTTGACCCTT TGTACCAACA GTCCCTGGTT
    TTTGATGAAA GTCCACAGGG TAAAGTCCTT CAGGTGATTG TCTGGGGGGA CTACGGCAGA
    ATGGACCACA AATGCTTCAT GGGGGTGGCT CAGATCTTGT TGGAGGAACT CGATCTTTCC
    AGCATGGTCA TCGGCTGGTA CAAGCTGTTC CCACCGTCAT CACTGGTCGA CCCCACGCTC
    ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA TCTCTGGAAA GTTCCACTGG GCCCCCCTGC
    ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852647.1
    CDS6..4637
    Translation

    Target ORF information:

    RefSeq Version XM_024996879.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGCACT CCAGAAAGTC TGAAAGATCT
    AGCATCCAAA GCCAGACTAG GAAAGGCACT GCCTCTGATG CAGAAAGGGT TCTCCCACCA
    TGTCTTTCTA GAAGGGGATA CGCAGCCGCG AGAGCGACTG ATCAGCCAGT CCTTAGGGGG
    AAACATCCCG CTCGCTCCCG CTCGAGCGAG CACCCTAGTG TCAGAACACT CTGCTCTATG
    CACCACCTTG CCCCCGGAGG GTCCGCGCCA CCTTCTCCGC TCCTGACAAG GACGCACCGG
    CAGGCGAGCC CCACCCGGTC CCCGCCCATG GACGCCGCCT TCAGCAGCCG CAGGGGCAGG
    CAGCTCCCGC AGGTGCCCGT GCGAAGCGGC AGCATAGAAC AAGCAAGCTT AGTAGTGGAG
    GAGCGAACGA GACAGATGAA AATGAAAGTG CATCGATTTA AGCAGACAAC AGGGTCTGGT
    TCTAGTCAAG AACTTGATCG CGAGCAATAT TCCAAGTATA ACGCACACAC AGATCAGTAC
    AGAAGCTGCG ACAACGTCTC CACCAGATCC TCAGACAGCG ACGTCAGCGA CGCGTCTGCC
    GTGTCCCGAA CCAGCAGTGC CTCCCGCCTC AGCAGCACCA GCTTCCTGTC CGAGCAGTCT
    GAGCGGCCTC GGGGCAGGAT CAGTTCATTT ACCCCTAAAA TGCAAGGCAG ACGGATGGGG
    ACTTCCGGAA GAGCCATCAC CAAGAGCACA AGCATGAGCG GAGAGATGTA CACACTGGAG
    CATAATGATG GCAGCCAGTC GGACACAGCT GTGGGCACCG TTGGAGCAGG TGGGAAGAAG
    AGGAGGTCCA GCCTGAGTGC CAAGGTGGTT GCCATAGTGT CTCGAAGGAG CAGAAGCACA
    TCCCAGCTGA GCCAAACAGA GTCAGGCCAC AAGAAGTTGA AAAGCACCAT CCAGAGAAGC
    ACTGAGACAG GAATGGCAGC TGAGATGAGG AAGATGGTGA GGCAGCCAAG CCGGGAGTCC
    ACGGACGGCA GCATCAACAG CTACAGCTCC GAGGGAAATT TAATATTTCC TGGAGTGAGA
    CTAGGCGCAG ATAGTCAATT CAGTGATTTT CTTGATGGAT TGGGACCAGC CCAGCTTGTT
    GGCCGCCAAA CCCTTGCTAC CCCTGCAATG GGTGATATAC AGATAGGAAT GGAGGATAAA
    AAGGGCCAGT TAGAAGTTGA AGTGATTCGA GCACGAAGCC TCACGCAGAA ACCCGGCTCC
    AAATCCACAC CTGCTCCTTA TGTCAAAGTG TATCTTTTGG AGAATGGGGC CTGCATAGCC
    AAGAAGAAGA CAAGAATTGC ACGAAAGACC CTTGACCCTT TGTACCAACA GTCCCTGGTT
    TTTGATGAAA GTCCACAGGG TAAAGTCCTT CAGGTGATTG TCTGGGGGGA CTACGGCAGA
    ATGGACCACA AATGCTTCAT GGGGGTGGCT CAGATCTTGT TGGAGGAACT CGATCTTTCC
    AGCATGGTCA TCGGCTGGTA CAAGCTGTTC CCACCGTCAT CACTGGTCGA CCCCACGCTC
    ACCCCTCTGA CCCGCCGGGC TTCCCAGTCA TCTCTGGAAA GTTCCACTGG GCCCCCCTGC
    ATCCGGTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237938
    Clone ID Related Accession (Same CDS sequence) XM_024996880.1
    Accession Version XM_024996880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852648.1
    CDS6..4634
    Translation

    Target ORF information:

    RefSeq Version XM_024996880.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGCACTCCA GAAAGTCTGA AAGATCTAGC
    ATCCAAAGCC AGACTAGGAA AGGCACTGCC TCTGATGCAG AAAGGGTTCT CCCACCATGT
    CTTTCTAGAA GGGGATACGC AGCCGCGAGA GCGACTGATC AGCCAGTCCT TAGGGGGAAA
    CATCCCGCTC GCTCCCGCTC GAGCGAGCAC CCTAGTGTCA GAACACTCTG CTCTATGCAC
    CACCTTGCCC CCGGAGGGTC CGCGCCACCT TCTCCGCTCC TGACAAGGAC GCACCGGCAG
    GCGAGCCCCA CCCGGTCCCC GCCCATGGAC GCCGCCTTCA GCAGCCGCAG GGGCAGGCAG
    CTCCCGCAGG TGCCCGTGCG AAGCGGCAGC ATAGAACAAG CAAGCTTAGT AGTGGAGGAG
    CGAACGAGAC AGATGAAAAT GAAAGTGCAT CGATTTAAGC AGACAACAGG GTCTGGTTCT
    AGTCAAGAAC TTGATCGCGA GCAATATTCC AAGTATAACG CACACACAGA TCAGTACAGA
    AGCTGCGACA ACGTCTCCAC CAGATCCTCA GACAGCGACG TCAGCGACGC GTCTGCCGTG
    TCCCGAACCA GCAGTGCCTC CCGCCTCAGC AGCACCAGCT TCCTGTCCGA GCAGTCTGAG
    CGGCCTCGGG GCAGGATCAG TTCATTTACC CCTAAAATGC AAGGCAGACG GATGGGGACT
    TCCGGAAGAG CCATCACCAA GAGCACAAGC ATGAGCGGAG AGATGTACAC ACTGGAGCAT
    AATGATGGCA GCCAGTCGGA CACAGCTGTG GGCACCGTTG GAGCAGGTGG GAAGAAGAGG
    AGGTCCAGCC TGAGTGCCAA GGTGGTTGCC ATAGTGTCTC GAAGGAGCAG AAGCACATCC
    CAGCTGAGCC AAACAGAGTC AGGCCACAAG AAGTTGAAAA GCACCATCCA GAGAAGCACT
    GAGACAGGAA TGGCAGCTGA GATGAGGAAG ATGGTGAGGC AGCCAAGCCG GGAGTCCACG
    GACGGCAGCA TCAACAGCTA CAGCTCCGAG GGAAATTTAA TATTTCCTGG AGTGAGACTA
    GGCGCAGATA GTCAATTCAG TGATTTTCTT GATGGATTGG GACCAGCCCA GCTTGTTGGC
    CGCCAAACCC TTGCTACCCC TGCAATGGGT GATATACAGA TAGGAATGGA GGATAAAAAG
    GGCCAGTTAG AAGTTGAAGT GATTCGAGCA CGAAGCCTCA CGCAGAAACC CGGCTCCAAA
    TCCACACCTG CTCCTTATGT CAAAGTGTAT CTTTTGGAGA ATGGGGCCTG CATAGCCAAG
    AAGAAGACAA GAATTGCACG AAAGACCCTT GACCCTTTGT ACCAACAGTC CCTGGTTTTT
    GATGAAAGTC CACAGGGTAA AGTCCTTCAG GTGATTGTCT GGGGGGACTA CGGCAGAATG
    GACCACAAAT GCTTCATGGG GGTGGCTCAG ATCTTGTTGG AGGAACTCGA TCTTTCCAGC
    ATGGTCATCG GCTGGTACAA GCTGTTCCCA CCGTCATCAC TGGTCGACCC CACGCTCACC
    CCTCTGACCC GCCGGGCTTC CCAGTCATCT CTGGAAAGTT CCACTGGGCC CCCCTGCATC
    CGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237939
    Clone ID Related Accession (Same CDS sequence) XM_024996881.1
    Accession Version XM_024996881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGCAAGCT TAGTAGTGGA GGAGCGAACG AGACAGATGA AAATGAAAGT GCATCGATTT
    AAGCAGACAA CAGGGTCTGG TTCTAGTCAA GAACTTGATC GCGAGCAATA TTCCAAGTAT
    AACGCACACA CAGATCAGTA CAGAAGCTGC GACAACGTCT CCACCAGATC CTCAGACAGC
    GACGTCAGCG ACGCGTCTGC CGTGTCCCGA ACCAGCAGTG CCTCCCGCCT CAGCAGCACC
    AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT CGGGGCAGGA TCAGTTCATT TACCCCTAAA
    ATGCAAGGCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852649.1
    CDS6..4538
    Translation

    Target ORF information:

    RefSeq Version XM_024996881.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGT AGGTTATAGG TCTAGCACTC GAGAAAGCAA ACCTTCGACA
    TTAACCGTGC CAGAGCAGCA GAGAACAACT CATCACCGCT CACGGTCGGT GTCTCCTCAT
    CGCGGCGACG ATCAGGGGAG GCCACGGTCA CGTTTACCAA ATGTGCCATT ACAGAGGAGT
    TTAGATGAAA TTCATCCAAC AAGAAGATCA CGTTCTCCAA CCAGACACCG TGATGCCTCC
    CGAAGCCCAA TTGATCAGAG AATCAGAGAT GTGGACAGTC AGTGTTTATC AGAACAAGAC
    AGTGAGCTTC TCATGCTTCC CAGAGCAAAA CGAGGACGAA GTGCAGAATG CCTACACACC
    ACCAGTGACC TGCAGCCCTC CCTTGACAGG GCGAGGAGTG CTAGTACCAA CTGCTTGAGA
    CCAGATGCTA GTTTGCATTC ACCAGAACGA GAAAGGGGTA GATGGTCCCC CTCCCTAGAT
    AGGAGACGAC CTCCTAGCCC CAGGATTCAA ATCCAGCATG CATCTCCGGA GAATGACAGG
    CACTCCAGAA AGTCTGAAAG ATCTAGCATC CAAAGCCAGA CTAGGAAAGG CACTGCCTCT
    GATGCAGAAA GGACGCACCG GCAGGCGAGC CCCACCCGGT CCCCGCCCAT GGACGCCGCC
    TTCAGCAGCC GCAGGGGCAG GCAGCTCCCG CAGGTGCCCG TGCGAAGCGG CAGCATAGAA
    CAAGCAAGCT TAGTAGTGGA GGAGCGAACG AGACAGATGA AAATGAAAGT GCATCGATTT
    AAGCAGACAA CAGGGTCTGG TTCTAGTCAA GAACTTGATC GCGAGCAATA TTCCAAGTAT
    AACGCACACA CAGATCAGTA CAGAAGCTGC GACAACGTCT CCACCAGATC CTCAGACAGC
    GACGTCAGCG ACGCGTCTGC CGTGTCCCGA ACCAGCAGTG CCTCCCGCCT CAGCAGCACC
    AGCTTCCTGT CCGAGCAGTC TGAGCGGCCT CGGGGCAGGA TCAGTTCATT TACCCCTAAA
    ATGCAAGGCA GACGGATGGG GACTTCCGGA AGAGCCATCA CCAAGAGCAC AAGCATGAGC
    GGAGAGATGT ACACACTGGA GCATAATGAT GGCAGCCAGT CGGACACAGC TGTGGGCACC
    GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC AGCCTGAGTG CCAAGGTGGT TGCCATAGTG
    TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG AGCCAAACAG AGTCAGGCCA CAAGAAGTTG
    AAAAGCACCA TCCAGAGAAG CACTGAGACA GGAATGGCAG CTGAGATGAG GAAGATGGTG
    AGGCAGCCAA GCCGGGAGTC CACGGACGGC AGCATCAACA GCTACAGCTC CGAGGGAAAT
    TTAATATTTC CTGGAGTGAG ACTAGGCGCA GATAGTCAAT TCAGTGATTT TCTTGATGGA
    TTGGGACCAG CCCAGCTTGT TGGCCGCCAA ACCCTTGCTA CCCCTGCAAT GGGTGATATA
    CAGATAGGAA TGGAGGATAA AAAGGGCCAG TTAGAAGTTG AAGTGATTCG AGCACGAAGC
    CTCACGCAGA AACCCGGCTC CAAATCCACA CCTGCTCCTT ATGTCAAAGT GTATCTTTTG
    GAGAATGGGG CCTGCATAGC CAAGAAGAAG ACAAGAATTG CACGAAAGAC CCTTGACCCT
    TTGTACCAAC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTCCT TCAGGTGATT
    GTCTGGGGGG ACTACGGCAG AATGGACCAC AAATGCTTCA TGGGGGTGGC TCAGATCTTG
    TTGGAGGAAC TCGATCTTTC CAGCATGGTC ATCGGCTGGT ACAAGCTGTT CCCACCGTCA
    TCACTGGTCG ACCCCACGCT CACCCCTCTG ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA
    AGTTCCACTG GGCCCCCCTG CATCCGGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237940
    Clone ID Related Accession (Same CDS sequence) XM_024996882.1
    Accession Version XM_024996882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGGGTAGAT GGTCCCCCTC CCTAGATAGG
    AGACGACCTC CTAGCCCCAG GATTCAAATC CAGCATGCAT CTCCGGAGAA TGACAGGCAC
    TCCAGAAAGT CTGAAAGATC TAGCATCCAA AGCCAGACTA GGAAAGGCAC TGCCTCTGAT
    GCAGAAAGGA CGCACCGGCA GGCGAGCCCC ACCCGGTCCC CGCCCATGGA CGCCGCCTTC
    AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG GTGCCCGTGC GAAGCGGCAG CATAGAACAA
    GCAAGCTTAG TAGTGGAGGA GCGAACGAGA CAGATGAAAA TGAAAGTGCA TCGATTTAAG
    CAGACAACAG GGTCTGGTTC TAGTCAAGAA CTTGATCGCG AGCAATATTC CAAGTATAAC
    GCACACACAG ATCAGTACAG AAGCTGCGAC AACGTCTCCA CCAGATCCTC AGACAGCGAC
    GTCAGCGACG CGTCTGCCGT GTCCCGAACC AGCAGTGCCT CCCGCCTCAG CAGCACCAGC
    TTCCTGTCCG AGCAGTCTGA GCGGCCTCGG GGCAGGATCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852650.1
    CDS6..4508
    Translation

    Target ORF information:

    RefSeq Version XM_024996882.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGCCCCA GGTGTGTGGT CAGGGACATG GTGAAGCCTG CTGCCTGCAA AGCCCCAAGA 
    AATGTTGAAC ACCAGCCCCA CCAGCCTCCA CCGAGATTGC ATCAACAATT TGAAAGCTAT
    AAAGAACAAG TGAGGAAAAT AGGGGAAGAA GCACGGCGCT ACCAAGGAGA GCACAAGGAC
    GATGCTCCGA CCTGCGGGAT TTGCCACAAG ACCAAGTTCG CCGACGGCTG TGGCCACCTG
    TGCTCCTACT GCCGCACCAA GTTCTGCGCG CGCTGTGGGG GCCGCGTGTC TCTGCGGTCC
    AGCAACGAGG ACAAAGTGGT TATGTGGGTA TGCAATTTGT GTCGAAAGCA ACAGGAAATC
    TTAACCAAGT CAGGGGCGTG GTTCTTCGGC AGCGGCCCTC AGCAGCCCAG TCAGGACGGA
    ACGCTCAGCG ACACGGCTAC AGGGGCGGGT TCCGAGGCGC CTAGAGAGAA GAAAGCCCGG
    CTCCAAGAGC GCTCGAGGTC GCAGACCCCC CTGAGCAGCG CCGCGGCCTC CGCCCAGGAC
    CAGGCTACCC CCGGAGCACA GGTGGACAGG ACCAAGGGCG CTGAGCCCGC CCCGCAAGCC
    GGGGGCCCTG AGCAGAAGCA GGCATCCTCC AGATCTAGAA GCGAGCCTCC CAGAGACAGG
    AAGAAGACCC CAGGGCTGGC TGAGCAGAAT GGCAAAGGCG CTCCAAAGGG CGAGCGCAAA
    CGCGCGCCCA GGACCTCGGC GCCTCCTGCA GAGGCGCCCG CGGAAGAACG GGAGCGGAAG
    GAAAGGCGGG AAAGCCGAAG GCTGGAGAAA GGCCGGTCCC AGGACTACTC CGTCGTGCCG
    GAGAAACGCG AGGAAGGCCC GGTGGCTGAA GCGGAGAAGC AGCGGAAAGA GGAGGAGTAC
    CAGACCCGGT ACCGCAGCGA CCCGAACCTG GCGCGGTACC CCGTCCAGCC GCCGCCCGAG
    GAGCAGCAGA TGCGCATGCA CGCGCGGGTG TCCCGCGCCC GGCACGAGCG GCGCCACAGC
    GACGTGTCCC TGCCGCGCAC CGAGGCGGCC GCGGGGCCCG AGGGCAGGCC CGGCCCGCGC
    GCGCCCCACG CCGCCCGGGG CTCGCCGCCG GCCTCGCCGC GCGCCTACTC GGCTGAGAGG
    ACCGCGGAGG CCCGGGCGCC AGGAGCCCAG CCGCTCGCCG ACTCCAGTCC GCCGGCGCCC
    CGGCCAGGCC CGGGCCCCGC GGAGGTCCCG GAGCCGCTCC GGAAGCAGAG CCGCCTGGAC
    CCCGGCTCGG CCGTCCTGGT GCGCAAGGCC AAGCGCGAGA AAGTGGAGAC CATGCTGCGC
    AACGACTCGC TCAGCTCCGA CCAGTCCGAG TCTGTGCGGC CGTCGCCGCC CAAGCCGCAC
    CGCGCCAAGA GGGGCGGCAA GCGGCGCCAA ATGTCCGTGA GCAGCTCGGA AGAAGAGGGT
    GTGTCAACGC CGGAGTACAC GAGCTGCGAG GACGTGGAGC TGGAGAGCGA GAGCGTCAGC
    GAGAAGGGTG ACTTGGATTA TTACTGGCTG GATCCCGCCA CGTGGCATAG CCGGGAAACA
    TCACCTATTA GCTCGCATCC TGTAACGTGG CAGCCTTCTA AAGAAGGGGA TCGATTAATT
    GGACGTGTTA TTCTTAACAA GAGAACAACC ATGCCCAAAG AATCAGGTGC ATTACTGGGT
    CTGAAAGTTG TTGGAGGAAA AATGACTGAC TTAGGACGCC TCGGTGCCTT CATCACCAAA
    GTAAAGAAGG GTAGCCTTGC AGATGTGGTT GGACACTTGA GAGCAGGGGA TGAAGTTCTA
    GAATGGAATG GTAAACCCCT GCCGGGAGCT ACAAATGAAG AAGTTTACAA CATTATTTTA
    GAATCAAAAT CAGAACCTCA AGTTGAAATT ATTGTTTCAA GGCCTATTGG TGACATTCCA
    AGGATTCCTG AGAGCTCCCA TCCACCACTG GAGTCCAGTT CTAGTTCCTT TGAGTCTCAG
    AAGATGGAAA GGCCTTCCAT TTCTGTAATT TCTCCCACAA GCCCTGGAGC TCTCAAAGAT
    GCCCCACAAG TATTACCAGG ACAACTTTCT GTGAAGCTGT GGTATGACAA GGTGGGCCAC
    CAACTGATTG TCAATGTTCT GCAAGCAACA GACCTACCCC CTAGAGTAGA TGGGCGTCCC
    AGGAATCCCT ATGTAAAAAT GTATTTTCTT CCAGATAGAA GTGATAAAAG TAAAAGGAGA
    ACCAAAACGG TAAAGAAAGT CCTCGAACCA AAGTGGAATC AGACTTTTGT CTATTCGCAC
    GTGCATCGTA GAGACTTTAG AGAACGGATG TTGGAAATAA CCGTGTGGGA CCAGCCCAGG
    GTGCAGGAAG AGGAGAGTGA GTTCCTTGGA GAGATCCTCA TAGAGTTGGA AACAGCGCTT
    CTAGACGATG AGCCACATTG GTATAAACTT CAGACTCACG ACGAATCTTC ACTACCTCTG
    CCTCAGCCAT CGCCCTTCAT GCCGCGGCGA CACACCCACG GCGAAAGCGC CAGCAAGAAG
    CTGCAAAGGT CCCAGCGAAT CAGTGACAGT GACATGTCAG ACTATGAGGT TGATGATGCG
    ATTGGAGTGG TCCCTCCAGG TTATAGGTCT AGCACTCGAG AAAGCAAACC TTCGACATTA
    ACCGTGCCAG AGCAGCAGAG AACAACTCAT CACCGCTCAC GGTCGGTGTC TCCTCATCGC
    GGCGACGATC AGGGGAGGCC ACGGTCACGT TTACCAAATG TGCCATTACA GAGGAGTTTA
    GATGAAATTC ATCCAACAAG AAGATCACGT TCTCCAACCA GACACCGTGA TGCCTCCCGA
    AGCCCAATTG ATCAGAGAAT CAGAGATGTG GACAGTCAGT GTTTATCAGA ACAAGACAGT
    GAGCTTCTCA TGCTTCCCAG AGCAAAACGA GGACGAAGTG CAGAATGCCT ACACACCACC
    AGTGACCTGC AGCCCTCCCT TGACAGGGCG AGGAGTGCTA GTACCAACTG CTTGAGACCA
    GATGCTAGTT TGCATTCACC AGAACGAGAA AGGGGTAGAT GGTCCCCCTC CCTAGATAGG
    AGACGACCTC CTAGCCCCAG GATTCAAATC CAGCATGCAT CTCCGGAGAA TGACAGGCAC
    TCCAGAAAGT CTGAAAGATC TAGCATCCAA AGCCAGACTA GGAAAGGCAC TGCCTCTGAT
    GCAGAAAGGA CGCACCGGCA GGCGAGCCCC ACCCGGTCCC CGCCCATGGA CGCCGCCTTC
    AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG GTGCCCGTGC GAAGCGGCAG CATAGAACAA
    GCAAGCTTAG TAGTGGAGGA GCGAACGAGA CAGATGAAAA TGAAAGTGCA TCGATTTAAG
    CAGACAACAG GGTCTGGTTC TAGTCAAGAA CTTGATCGCG AGCAATATTC CAAGTATAAC
    GCACACACAG ATCAGTACAG AAGCTGCGAC AACGTCTCCA CCAGATCCTC AGACAGCGAC
    GTCAGCGACG CGTCTGCCGT GTCCCGAACC AGCAGTGCCT CCCGCCTCAG CAGCACCAGC
    TTCCTGTCCG AGCAGTCTGA GCGGCCTCGG GGCAGGATCA GACGGATGGG GACTTCCGGA
    AGAGCCATCA CCAAGAGCAC AAGCATGAGC GGAGAGATGT ACACACTGGA GCATAATGAT
    GGCAGCCAGT CGGACACAGC TGTGGGCACC GTTGGAGCAG GTGGGAAGAA GAGGAGGTCC
    AGCCTGAGTG CCAAGGTGGT TGCCATAGTG TCTCGAAGGA GCAGAAGCAC ATCCCAGCTG
    AGCCAAACAG AGTCAGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGAAG CACTGAGACA
    GGAATGGCAG CTGAGATGAG GAAGATGGTG AGGCAGCCAA GCCGGGAGTC CACGGACGGC
    AGCATCAACA GCTACAGCTC CGAGGGAAAT TTAATATTTC CTGGAGTGAG ACTAGGCGCA
    GATAGTCAAT TCAGTGATTT TCTTGATGGA TTGGGACCAG CCCAGCTTGT TGGCCGCCAA
    ACCCTTGCTA CCCCTGCAAT GGGTGATATA CAGATAGGAA TGGAGGATAA AAAGGGCCAG
    TTAGAAGTTG AAGTGATTCG AGCACGAAGC CTCACGCAGA AACCCGGCTC CAAATCCACA
    CCTGCTCCTT ATGTCAAAGT GTATCTTTTG GAGAATGGGG CCTGCATAGC CAAGAAGAAG
    ACAAGAATTG CACGAAAGAC CCTTGACCCT TTGTACCAAC AGTCCCTGGT TTTTGATGAA
    AGTCCACAGG GTAAAGTCCT TCAGGTGATT GTCTGGGGGG ACTACGGCAG AATGGACCAC
    AAATGCTTCA TGGGGGTGGC TCAGATCTTG TTGGAGGAAC TCGATCTTTC CAGCATGGTC
    ATCGGCTGGT ACAAGCTGTT CCCACCGTCA TCACTGGTCG ACCCCACGCT CACCCCTCTG
    ACCCGCCGGG CTTCCCAGTC ATCTCTGGAA AGTTCCACTG GGCCCCCCTG CATCCGGTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237964
    Clone ID Related Accession (Same CDS sequence) XM_024996907.1
    Accession Version XM_024996907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulating synaptic membrane exocytosis protein 1 isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCTCGG CCGTGGGGCC CCGGGGTCCC CGCCCGCCCA CCGTGCCTCC CCCGATGCCC 
    GAGCTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATCATCCT GGCGGTGATG
    GACCGGCAGA AGGAGGAAGA GGAGAAGGAA GAAGCCATGC TCAAGTGTGT GGTCAGGGAC
    ATGGTGAAGC CTGCTGCCTG CAAAGCCCCA AGAAATGTTG AACACCAGCC CCACCAGCCT
    CCACCGAGAT TGCATCAACA ATTTGAAAGC TATAAAGAAC AAGTGAGGAA AATAGGGGAA
    GAAGCACGGC GCTACCAAGG AGAGCACAAG GACGATGCTC CGACCTGCGG GATTTGCCAC
    AAGACCAAGT TCGCCGACGG CTGTGGCCAC CTGTGCTCCT ACTGCCGCAC CAAGTTCTGC
    GCGCGCTGTG GGGGCCGCGT GTCTCTGCGG TCCAGCAACG AGGACAAAGT GGTTATGTGG
    GTATGCAATT TGTGTCGAAA GCAACAGGAA ATCTTAACCA AGTCAGGGGC GTGGTTCTTC
    GGCAGCGGCC CTCAGCAGCC CAGTCAGGAC GGAACGCTCA GCGACACGGC TACAGGGGCG
    GGTTCCGAGG CGCCTAGAGA GAAGAAAGCC CGGCTCCAAG AGCGCTCGAG GTCGCAGACC
    CCCCTGAGCA GCGCCGCGGC CTCCGCCCAG GACCAGGCTA CCCCCGGAGC ACAGGTGGAC
    AGGACCAAGG GCGCTGAGCC CGCCCCGCAA GCCGGGGGCC CTGAGCAGAA GCAGGCATCC
    TCCAGATCTA GAAGCGAGCC TCCCAGAGAC AGGAAGAAGA CCCCAGGGCT GGCTGAGCAG
    AATGGCAAAG GCGCTCCAAA GGGCGAGCGC AAACGCGCGC CCAGGACCTC GGCGCCTCCT
    GCAGAGGCGC CCGCGGAAGA ACGGGAGCGG AAGGAAAGGC GGGAAAGCCG AAGGCTGGAG
    AAAGGCCGGT CCCAGGACTA CTCCGTCGTG CCGGAGAAAC GCGAGGAAGG CCCGGTGGCT
    GAAGCGGAGA AGCAGCGGAA AGAGGAGGAG TACCAGACCC GGTACCGCAG CGACCCGAAC
    CTGGCGCGGT ACCCCGTCCA GCCGCCGCCC GAGGAGCAGC AGATGCGCAT GCACGCGCGG
    GTGTCCCGCG CCCGGCACGA GCGGCGCCAC AGCGACGTGT CCCTGCCGCG CACCGAGGCG
    GCCGCGGGGC CCGAGGGCAG GCCCGGCCCG CGCGCGCCCC ACGCCGCCCG GGGCTCGCCG
    CCGGCCTCGC CGCGCGCCTA CTCGGCTGAG AGGACCGCGG AGGCCCGGGC GCCAGGAGCC
    CAGCCGCTCG CCGACTCCAG TCCGCCGGCG CCCCGGCCAG GCCCGGGCCC CGCGGAGGTC
    CCGGAGCCGC TCCGGAAGCA GAGCCGCCTG GACCCCGGCT CGGCCGTCCT GGTGCGCAAG
    GCCAAGCGCG AGAAAGTGGA GACCATGCTG CGCAACGACT CGCTCAGCTC CGACCAGTCC
    GAGTCTGTGC GGCCGTCGCC GCCCAAGCCG CACCGCGCCA AGAGGGGCGG CAAGCGGCGC
    CAAATGTCCG TGAGCAGCTC GGAAGAAGAG GGTGTGTCAA CGCCGGAGTA CACGAGCTGC
    GAGGACGTGG AGCTGGAGAG CGAGAGCGTC AGCGAGAAGG GTGACTTGGA TTATTACTGG
    CTGGATCCCG CCACGTGGCA TAGCCGGGAA ACATCACCTA TTAGCTCGCA TCCTGTAACG
    TGGCAGCCTT CTAAAGAAGG GGATCGATTA ATTGGACGTG TTATTCTTAA CAAGAGAACA
    ACCATGCCCA AAGAATCAGG TGCATTACTG GGTCTGAAAG TTGTTGGAGG AAAAATGACT
    GACTTAGGAC GCCTCGGTGC CTTCATCACC AAAGTAAAGA AGGGTAGCCT TGCAGATGTG
    GTTGGACACT TGAGAGCAGG GGATGAAGTT CTAGAATGGA ATGGTAAACC CCTGCCGGGA
    GCTACAAATG AAGAAGTTTA CAACATTATT TTAGAATCAA AATCAGAACC TCAAGTTGAA
    ATTATTGTTT CAAGGCCTAT TGGTGACATT CCAAGGATTC CTGAGAGCTC CCATCCACCA
    CTGGAGTCCA GTTCTAGTTC CTTTGAGTCT CAGAAGATGG AAAGGCCTTC CATTTCTGTA
    ATTTCTCCCA CAAGCCCTGG AGCTCTCAAA GATGCCCCAC AAGTATTACC AGGACAACTT
    TCTGTGAAGC TGTGGTATGA CAAGGTGGGC CACCAACTGA TTGTCAATGT TCTGCAAGCA
    ACAGACCTAC CCCCTAGAGT AGATGGGCGT CCCAGGAATC CCTATGTAAA AATGTATTTT
    CTTCCAGATA GAAGTGATAA AAGTAAAAGG AGAACCAAAA CGGTAAAGAA AGTCCTCGAA
    CCAAAGTGGA ATCAGACTTT TGTCTATTCG CACGTGCATC GTAGAGACTT TAGAGAACGG
    ATGTTGGAAA TAACCGTGTG GGACCAGCCC AGGGTGCAGG AAGAGGAGAG TGAGTTCCTT
    GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTCTAGACG ATGAGCCACA TTGGTATAAA
    CTTCAGACTC ACGACGAATC TTCACTACCT CTGCCTCAGC CATCGCCCTT CATGCCGCGG
    CGACACACCC ACGGCGAAAG CGCCAGCAAG AAGCTGCAAA GGTCCCAGCG AATCAGTGAC
    AGTGACATGT CAGACTATGA GGTTGATGAT GCGATTGGAG TGGTCCCTCC AGTAGGTTAT
    AGGTCTAGCA CTCGAGAAAG CAAACCTTCG ACATTAACCG TGCCAGAGCA GCAGAGAACA
    ACTCATCACC GCTCACGGTC GGTGTCTCCT CATCGCGGCG ACGATCAGGG GAGGCCACGG
    TCACGTTTAC CAAATGTGCC ATTACAGAGG AGTTTAGATG AAATTCATCC AACAAGAAGA
    TCACGTTCTC CAACCAGACA CCGTGATGCC TCCCGAAGCC CAATTGATCA GAGAATCAGA
    GATGTGGACA GTCAGTGTTT ATCAGAACAA GACAGTGAGC TTCTCATGCT TCCCAGAGCA
    AAACGAGGAC GAAGTGCAGA ATGCCTACAC ACCACCAGAC ATCTTGTTAG GCACTATAAA
    ACATTACCGC CTAAGATGCC CTTATTAGAG ACCTGTTCTC ACTGGGATAT TTACAGCTCA
    ATTCTGCCTG CACGTACCAA AACCCAAACC CTTCACCATG AATGTTTTCA CTCGAGAGCA
    CTGAGGTTTA CTGATGAGAT TCTGGTTGGT GACCTGCAGC CCTCCCTTGA CAGGGCGAGG
    AGTGCTAGTA CCAACTGCTT GAGACCAGAT GCTAGTTTGC ATTCACCAGA ACGAGAAAGG
    GGTAGATGGT CCCCCTCCCT AGATAGGAGA CGACCTCCTA GCCCCAGGAT TCAAATCCAG
    CATGCATCTC CGGAGAATGA CAGGCACTCC AGAAAGTCTG AAAGATCTAG CATCCAAAGC
    CAGACTAGGA AAGGCACTGC CTCTGATGCA GAAAGGGTTC TCCCACCATG TCTTTCTAGA
    AGGGGATACG CAGCCGCGAG AGCGACTGAT CAGCCAGTCC TTAGGGGGAA ACATCCCGCT
    CGCTCCCGCT CGAGCGAGCA CCCTAGTGTC AGAACACTCT GCTCTATGCA CCACCTTGCC
    CCCGGAGGGT CCGCGCCACC TTCTCCGCTC CTGACAAGGA CGCACCGGCA GGCGAGCCCC
    ACCCGGTCCC CGCCCATGGA CGCCGCCTTC AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG
    GTGCCCGTGC GAAGCGGCAG CATAGAACAA GCAAGCTTAG TAGTGGAGGA GCGAACGAGA
    CAGATGAAAA TGAAAGTGCA TCGATTTAAG CAGACAACAG GGTCTGGTTC TAGTCAAGAA
    CTTGATCGCG AGCAATATTC CAAGTATAAC GCACACACAG ATCAGTACAG AAGCTGCGAC
    AACGTCTCCA CCAGATCCTC AGACAGCGAC GTCAGCGACG CGTCTGCCGT GTCCCGAACC
    AGCAGTGCCT CCCGCCTCAG CAGCACCAGC TTCCTGTCCG AGCAGTCTGA GCGGCCTCGG
    GGCAGGATCA GTTCATTTAC CCCTAAAATG CAAGGCAGAC GGATGGGGAC TTCCGGAAGA
    GCCATCACCA AGAGCACAAG CATGAGCGGA GAGATGTACA CACTGGAGCA TAATGATGGC
    AGCCAGTCGG ACACAGCTGT GGGCACCGTT GGAGCAGGTG GGAAGAAGAG GAGGTCCAGC
    CTGAGTGCCA AGGTGGTTGC CATAGTGTCT CGAAGGAGCA GAAGCACATC CCAGCTGAGC
    CAAACAGAGT CAGGCCACAA GAAGTTGAAA AGCACCATCC AGAGAAGCAC TGAGACAGGA
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852675.1
    CDS262..5301
    Translation

    Target ORF information:

    RefSeq Version XM_024996907.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCTCGG CCGTGGGGCC CCGGGGTCCC CGCCCGCCCA CCGTGCCTCC CCCGATGCCC 
    GAGCTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATCATCCT GGCGGTGATG
    GACCGGCAGA AGGAGGAAGA GGAGAAGGAA GAAGCCATGC TCAAGTGTGT GGTCAGGGAC
    ATGGTGAAGC CTGCTGCCTG CAAAGCCCCA AGAAATGTTG AACACCAGCC CCACCAGCCT
    CCACCGAGAT TGCATCAACA ATTTGAAAGC TATAAAGAAC AAGTGAGGAA AATAGGGGAA
    GAAGCACGGC GCTACCAAGG AGAGCACAAG GACGATGCTC CGACCTGCGG GATTTGCCAC
    AAGACCAAGT TCGCCGACGG CTGTGGCCAC CTGTGCTCCT ACTGCCGCAC CAAGTTCTGC
    GCGCGCTGTG GGGGCCGCGT GTCTCTGCGG TCCAGCAACG AGGACAAAGT GGTTATGTGG
    GTATGCAATT TGTGTCGAAA GCAACAGGAA ATCTTAACCA AGTCAGGGGC GTGGTTCTTC
    GGCAGCGGCC CTCAGCAGCC CAGTCAGGAC GGAACGCTCA GCGACACGGC TACAGGGGCG
    GGTTCCGAGG CGCCTAGAGA GAAGAAAGCC CGGCTCCAAG AGCGCTCGAG GTCGCAGACC
    CCCCTGAGCA GCGCCGCGGC CTCCGCCCAG GACCAGGCTA CCCCCGGAGC ACAGGTGGAC
    AGGACCAAGG GCGCTGAGCC CGCCCCGCAA GCCGGGGGCC CTGAGCAGAA GCAGGCATCC
    TCCAGATCTA GAAGCGAGCC TCCCAGAGAC AGGAAGAAGA CCCCAGGGCT GGCTGAGCAG
    AATGGCAAAG GCGCTCCAAA GGGCGAGCGC AAACGCGCGC CCAGGACCTC GGCGCCTCCT
    GCAGAGGCGC CCGCGGAAGA ACGGGAGCGG AAGGAAAGGC GGGAAAGCCG AAGGCTGGAG
    AAAGGCCGGT CCCAGGACTA CTCCGTCGTG CCGGAGAAAC GCGAGGAAGG CCCGGTGGCT
    GAAGCGGAGA AGCAGCGGAA AGAGGAGGAG TACCAGACCC GGTACCGCAG CGACCCGAAC
    CTGGCGCGGT ACCCCGTCCA GCCGCCGCCC GAGGAGCAGC AGATGCGCAT GCACGCGCGG
    GTGTCCCGCG CCCGGCACGA GCGGCGCCAC AGCGACGTGT CCCTGCCGCG CACCGAGGCG
    GCCGCGGGGC CCGAGGGCAG GCCCGGCCCG CGCGCGCCCC ACGCCGCCCG GGGCTCGCCG
    CCGGCCTCGC CGCGCGCCTA CTCGGCTGAG AGGACCGCGG AGGCCCGGGC GCCAGGAGCC
    CAGCCGCTCG CCGACTCCAG TCCGCCGGCG CCCCGGCCAG GCCCGGGCCC CGCGGAGGTC
    CCGGAGCCGC TCCGGAAGCA GAGCCGCCTG GACCCCGGCT CGGCCGTCCT GGTGCGCAAG
    GCCAAGCGCG AGAAAGTGGA GACCATGCTG CGCAACGACT CGCTCAGCTC CGACCAGTCC
    GAGTCTGTGC GGCCGTCGCC GCCCAAGCCG CACCGCGCCA AGAGGGGCGG CAAGCGGCGC
    CAAATGTCCG TGAGCAGCTC GGAAGAAGAG GGTGTGTCAA CGCCGGAGTA CACGAGCTGC
    GAGGACGTGG AGCTGGAGAG CGAGAGCGTC AGCGAGAAGG GTGACTTGGA TTATTACTGG
    CTGGATCCCG CCACGTGGCA TAGCCGGGAA ACATCACCTA TTAGCTCGCA TCCTGTAACG
    TGGCAGCCTT CTAAAGAAGG GGATCGATTA ATTGGACGTG TTATTCTTAA CAAGAGAACA
    ACCATGCCCA AAGAATCAGG TGCATTACTG GGTCTGAAAG TTGTTGGAGG AAAAATGACT
    GACTTAGGAC GCCTCGGTGC CTTCATCACC AAAGTAAAGA AGGGTAGCCT TGCAGATGTG
    GTTGGACACT TGAGAGCAGG GGATGAAGTT CTAGAATGGA ATGGTAAACC CCTGCCGGGA
    GCTACAAATG AAGAAGTTTA CAACATTATT TTAGAATCAA AATCAGAACC TCAAGTTGAA
    ATTATTGTTT CAAGGCCTAT TGGTGACATT CCAAGGATTC CTGAGAGCTC CCATCCACCA
    CTGGAGTCCA GTTCTAGTTC CTTTGAGTCT CAGAAGATGG AAAGGCCTTC CATTTCTGTA
    ATTTCTCCCA CAAGCCCTGG AGCTCTCAAA GATGCCCCAC AAGTATTACC AGGACAACTT
    TCTGTGAAGC TGTGGTATGA CAAGGTGGGC CACCAACTGA TTGTCAATGT TCTGCAAGCA
    ACAGACCTAC CCCCTAGAGT AGATGGGCGT CCCAGGAATC CCTATGTAAA AATGTATTTT
    CTTCCAGATA GAAGTGATAA AAGTAAAAGG AGAACCAAAA CGGTAAAGAA AGTCCTCGAA
    CCAAAGTGGA ATCAGACTTT TGTCTATTCG CACGTGCATC GTAGAGACTT TAGAGAACGG
    ATGTTGGAAA TAACCGTGTG GGACCAGCCC AGGGTGCAGG AAGAGGAGAG TGAGTTCCTT
    GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTCTAGACG ATGAGCCACA TTGGTATAAA
    CTTCAGACTC ACGACGAATC TTCACTACCT CTGCCTCAGC CATCGCCCTT CATGCCGCGG
    CGACACACCC ACGGCGAAAG CGCCAGCAAG AAGCTGCAAA GGTCCCAGCG AATCAGTGAC
    AGTGACATGT CAGACTATGA GGTTGATGAT GCGATTGGAG TGGTCCCTCC AGTAGGTTAT
    AGGTCTAGCA CTCGAGAAAG CAAACCTTCG ACATTAACCG TGCCAGAGCA GCAGAGAACA
    ACTCATCACC GCTCACGGTC GGTGTCTCCT CATCGCGGCG ACGATCAGGG GAGGCCACGG
    TCACGTTTAC CAAATGTGCC ATTACAGAGG AGTTTAGATG AAATTCATCC AACAAGAAGA
    TCACGTTCTC CAACCAGACA CCGTGATGCC TCCCGAAGCC CAATTGATCA GAGAATCAGA
    GATGTGGACA GTCAGTGTTT ATCAGAACAA GACAGTGAGC TTCTCATGCT TCCCAGAGCA
    AAACGAGGAC GAAGTGCAGA ATGCCTACAC ACCACCAGAC ATCTTGTTAG GCACTATAAA
    ACATTACCGC CTAAGATGCC CTTATTAGAG ACCTGTTCTC ACTGGGATAT TTACAGCTCA
    ATTCTGCCTG CACGTACCAA AACCCAAACC CTTCACCATG AATGTTTTCA CTCGAGAGCA
    CTGAGGTTTA CTGATGAGAT TCTGGTTGGT GACCTGCAGC CCTCCCTTGA CAGGGCGAGG
    AGTGCTAGTA CCAACTGCTT GAGACCAGAT GCTAGTTTGC ATTCACCAGA ACGAGAAAGG
    GGTAGATGGT CCCCCTCCCT AGATAGGAGA CGACCTCCTA GCCCCAGGAT TCAAATCCAG
    CATGCATCTC CGGAGAATGA CAGGCACTCC AGAAAGTCTG AAAGATCTAG CATCCAAAGC
    CAGACTAGGA AAGGCACTGC CTCTGATGCA GAAAGGGTTC TCCCACCATG TCTTTCTAGA
    AGGGGATACG CAGCCGCGAG AGCGACTGAT CAGCCAGTCC TTAGGGGGAA ACATCCCGCT
    CGCTCCCGCT CGAGCGAGCA CCCTAGTGTC AGAACACTCT GCTCTATGCA CCACCTTGCC
    CCCGGAGGGT CCGCGCCACC TTCTCCGCTC CTGACAAGGA CGCACCGGCA GGCGAGCCCC
    ACCCGGTCCC CGCCCATGGA CGCCGCCTTC AGCAGCCGCA GGGGCAGGCA GCTCCCGCAG
    GTGCCCGTGC GAAGCGGCAG CATAGAACAA GCAAGCTTAG TAGTGGAGGA GCGAACGAGA
    CAGATGAAAA TGAAAGTGCA TCGATTTAAG CAGACAACAG GGTCTGGTTC TAGTCAAGAA
    CTTGATCGCG AGCAATATTC CAAGTATAAC GCACACACAG ATCAGTACAG AAGCTGCGAC
    AACGTCTCCA CCAGATCCTC AGACAGCGAC GTCAGCGACG CGTCTGCCGT GTCCCGAACC
    AGCAGTGCCT CCCGCCTCAG CAGCACCAGC TTCCTGTCCG AGCAGTCTGA GCGGCCTCGG
    GGCAGGATCA GTTCATTTAC CCCTAAAATG CAAGGCAGAC GGATGGGGAC TTCCGGAAGA
    GCCATCACCA AGAGCACAAG CATGAGCGGA GAGATGTACA CACTGGAGCA TAATGATGGC
    AGCCAGTCGG ACACAGCTGT GGGCACCGTT GGAGCAGGTG GGAAGAAGAG GAGGTCCAGC
    CTGAGTGCCA AGGTGGTTGC CATAGTGTCT CGAAGGAGCA GAAGCACATC CCAGCTGAGC
    CAAACAGAGT CAGGCCACAA GAAGTTGAAA AGCACCATCC AGAGAAGCAC TGAGACAGGA
    ATGGCAGCTG AGATGAGGAA GATGGTGAGG CAGCCAAGCC GGGAGTCCAC GGACGGCAGC
    ATCAACAGCT ACAGCTCCGA GGGAAATTTA ATATTTCCTG GAGTGAGACT AGGCGCAGAT
    AGTCAATTCA GTGATTTTCT TGATGGATTG GGACCAGCCC AGCTTGTTGG CCGCCAAACC
    CTTGCTACCC CTGCAATGGG TGATATACAG ATAGGAATGG AGGATAAAAA GGGCCAGTTA
    GAAGTTGAAG TGATTCGAGC ACGAAGCCTC ACGCAGAAAC CCGGCTCCAA ATCCACACCT
    GCTCCTTATG TCAAAGTGTA TCTTTTGGAG AATGGGGCCT GCATAGCCAA GAAGAAGACA
    AGAATTGCAC GAAAGACCCT TGACCCTTTG TACCAACAGT CCCTGGTTTT TGATGAAAGT
    CCACAGGGTA AAGTCCTTCA GGTGATTGTC TGGGGGGACT ACGGCAGAAT GGACCACAAA
    TGCTTCATGG GGGTGGCTCA GATCTTGTTG GAGGAACTCG ATCTTTCCAG CATGGTCATC
    GGCTGGTACA AGCTGTTCCC ACCGTCATCA CTGGTCGACC CCACGCTCAC CCCTCTGACC
    CGCCGGGCTT CCCAGTCATC TCTGGAAAGT TCCACTGGGC CCCCCTGCAT CCGGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.