EWSR1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EWSR1
Entrez Gene ID	534073
Full Name	EWS RNA binding protein 1
General protein information	Preferred Names EWS RNA binding protein 1 Names RNA-binding protein EWS Ewing sarcoma breakpoint region 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005218075.3	XP_005218132.1	RNA-binding protein EWS isoform X1
XM_005218076.3	XP_005218133.1	RNA-binding protein EWS isoform X2
XM_005218077.3	XP_005218134.1	RNA-binding protein EWS isoform X3
XM_005218076.4	XP_005218133.1	RNA-binding protein EWS isoform X2
XM_005218077.4	XP_005218134.1	RNA-binding protein EWS isoform X3
XM_005218075.4	XP_005218132.1	RNA-binding protein EWS isoform X1
NM_001109800.2	NP_001103270.1	RNA-binding protein EWS

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EWSR1	XP_001173113.2
Bos taurus (cattle)	EWSR1	NP_001103270.1
Rattus norvegicus (Norway rat)	Ewsr1	NP_001020803.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of bovine transcripts preferentially expressed in testis and with a putative role in spermatogenesis.
Alves BC, Tobo PR, Rodrigues R, Ruiz JC, de Lima VF, Moreira-Filho CA
Theriogenology76(6)991-8(2011 Oct)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EWSR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EWSR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36657	NM_001109800.2 Latest version!	Bos taurus EWS RNA binding protein 1 (EWSR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa28036	XM_005218075.4 Latest version!	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa28037	XM_005218076.4 Latest version!	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa28038	XM_005218077.4 Latest version!	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa28037	XM_005218076.3	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa28038	XM_005218077.3	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa28036	XM_005218075.3	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EWSR1 ( NM_001109800.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001103270.1 Bos taurus EWS RNA binding protein 1 (EWSR1), mRNA.

Price & Availability↑

CloneID	OBa36657
Clone ID Related Accession (Same CDS sequence)	NM_001109800.2
Accession Version	NM_001109800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	RNA-binding protein EWS
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153844.1. On Aug 30, 2012 this sequence version replaced NM_001109800.1. ##Evidence-Data-START## Transcript exon combination :: BC153844.1, BC149284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGGCATATGG GCAGCAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGATA TTCTACTCCA 
GCTGCCCCCC AGGCATACAG TCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGACACC 
ACCACTGCTA CGGTTACTAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT 
CAGCCCGCTT ACCCGGCGTA TGGACAGCAG CCAGCAGCCA CCGCGCCTGC AAGACCGCAG 
GATGGTAACA AACCCGCTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG 
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTGCCTGG GAGCTACCCC 
ATGCAGCCGG TCTCAGCACC ACCATCCTAT CCTCCTACCA GCTACTCCTC TACGCAGCCG 
ACTAGTTATG ATCAGAGCAG TTACTCCCAG CAGAACACCT ACGGGCAGCC GAGCAGCTAT 
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCACC CACTAGTTAC 
CCCCCCCAGA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGTCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCGCTGG AGAGCGAGGT GGCTTCAATA AGCCTGGTGG ACCCATGGAT 
GAAGGACCAG ACCTTGATTT AGGCCCACCT GTAGATCCAG ATGAAGACTC TGACAACAGT 
GCCATTTATG TGCAAGGCCT GAACGACAGT GTGACTCTCG ATGACCTGGT GGACTTCTTT 
AAGCAGTGTG GAGTCGTTAA GATGAACAAG AGGACTGGAC AACCCATGAT CCATATTTAC 
TTGGACAAGG AAACAGGAAA GCCCAAAGGC GATGCTACGG TGTCTTACGA AGACCCAGCA 
ACTGCCAAAG CTGCTGTCGA GTGGTTTGAT GGGAAAGATT TCCAAGGGAG CAAACTTAAA 
GTGTCTCTTG CTCGGAAGAA GCCGCCCATG AACAGCATGC GGGGAGGGAT GCCGCCGCGC 
GAGGGCAGGG GGATGCCGCC GCCGCTCCGA GGAGGTCCAG GGGGCCCAGG AGGTCCTGGA 
GGACCCATGG GGCGCATGGG AGGCCGCGGA GGAGACAGAG GCGGCTTCCC ACCAAGAGGG 
CCTCGCGGTT CCCGGGGGAA CCCGTCTGGA GGAGGAAACG TCCAGCACCG AGCTGGAGAC 
TGGCAGTGCC CCAACCCGGG CTGTGGGAAC CAGAACTTCG CCTGGAGAAC AGAGTGCAAC 
CAGTGTAAGG CCCCAAAGCC TGAAGGCTTC CTCCCACCGC CTTTCCCGCC CCCAGGCGGT 
GACCGTGGCA GAGGTGGCCC TGGAGGCATG CGGGGAGGAA GAGGTGGCCT CATGGACCGC 
GGTGGTCCTG GTGGAATGTT CAGAGGCGGT CGTGGTGGAG ACAGAGGTGG CTTCCGTGGC 
GGCCGGGGCA TGGACCGCGG TGGCTTTGGT GGTGGAAGAC GAGGTGGCCC TGGGGGACCC 
CCTGGACCAC TGATGGAACA GATGGGAGGA CGACGAGGCG GGCGAGGAGG ACCTGGAAAG 
ATGGATAAAG GCGAGCACCG TCAGGAGCGC AGAGACCGGC CCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103270.1
CDS	56..2023
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVSAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLVDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPATAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	NM_001109800.2
Organism	Bos taurus(cattle)
Definition	Bos taurus EWS RNA binding protein 1 (EWSR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109800.2

ORF Insert Sequence:

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGGCATATGG GCAGCAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGATA TTCTACTCCA 
GCTGCCCCCC AGGCATACAG TCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGACACC 
ACCACTGCTA CGGTTACTAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT 
CAGCCCGCTT ACCCGGCGTA TGGACAGCAG CCAGCAGCCA CCGCGCCTGC AAGACCGCAG 
GATGGTAACA AACCCGCTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG 
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTGCCTGG GAGCTACCCC 
ATGCAGCCGG TCTCAGCACC ACCATCCTAT CCTCCTACCA GCTACTCCTC TACGCAGCCG 
ACTAGTTATG ATCAGAGCAG TTACTCCCAG CAGAACACCT ACGGGCAGCC GAGCAGCTAT 
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCACC CACTAGTTAC 
CCCCCCCAGA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGTCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCGCTGG AGAGCGAGGT GGCTTCAATA AGCCTGGTGG ACCCATGGAT 
GAAGGACCAG ACCTTGATTT AGGCCCACCT GTAGATCCAG ATGAAGACTC TGACAACAGT 
GCCATTTATG TGCAAGGCCT GAACGACAGT GTGACTCTCG ATGACCTGGT GGACTTCTTT 
AAGCAGTGTG GAGTCGTTAA GATGAACAAG AGGACTGGAC AACCCATGAT CCATATTTAC 
TTGGACAAGG AAACAGGAAA GCCCAAAGGC GATGCTACGG TGTCTTACGA AGACCCAGCA 
ACTGCCAAAG CTGCTGTCGA GTGGTTTGAT GGGAAAGATT TCCAAGGGAG CAAACTTAAA 
GTGTCTCTTG CTCGGAAGAA GCCGCCCATG AACAGCATGC GGGGAGGGAT GCCGCCGCGC 
GAGGGCAGGG GGATGCCGCC GCCGCTCCGA GGAGGTCCAG GGGGCCCAGG AGGTCCTGGA 
GGACCCATGG GGCGCATGGG AGGCCGCGGA GGAGACAGAG GCGGCTTCCC ACCAAGAGGG 
CCTCGCGGTT CCCGGGGGAA CCCGTCTGGA GGAGGAAACG TCCAGCACCG AGCTGGAGAC 
TGGCAGTGCC CCAACCCGGG CTGTGGGAAC CAGAACTTCG CCTGGAGAAC AGAGTGCAAC 
CAGTGTAAGG CCCCAAAGCC TGAAGGCTTC CTCCCACCGC CTTTCCCGCC CCCAGGCGGT 
GACCGTGGCA GAGGTGGCCC TGGAGGCATG CGGGGAGGAA GAGGTGGCCT CATGGACCGC 
GGTGGTCCTG GTGGAATGTT CAGAGGCGGT CGTGGTGGAG ACAGAGGTGG CTTCCGTGGC 
GGCCGGGGCA TGGACCGCGG TGGCTTTGGT GGTGGAAGAC GAGGTGGCCC TGGGGGACCC 
CCTGGACCAC TGATGGAACA GATGGGAGGA CGACGAGGCG GGCGAGGAGG ACCTGGAAAG 
ATGGATAAAG GCGAGCACCG TCAGGAGCGC AGAGACCGGC CCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EWSR1 ( XM_005218075.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218132.1 Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28036
Clone ID Related Accession (Same CDS sequence)	XM_005218075.3 , XM_005218075.4
Accession Version	XM_005218075.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	RNA-binding protein EWS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC 
ATGGATGAAG GACCAGACCT TGATTTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC 
AACAGTGCCA TTTATGTGCA AGGCCTGAAC GACAGTGTGA CTCTCGATGA CCTGGTGGAC 
TTCTTTAAGC AGTGTGGAGT CGTTAAGATG AACAAGAGGA CTGGACAACC CATGATCCAT 
ATTTACTTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CTACGGTGTC TTACGAAGAC 
CCAGCAACTG CCAAAGCTGC TGTCGAGTGG TTTGATGGGA AAGATTTCCA AGGGAGCAAA 
CTTAAAGTGT CTCTTGCTCG GAAGAAGCCG CCCATGAACA GCATGCGGGG AGGGATGCCG 
CCGCGCGAGG GCAGGGGGAT GCCGCCGCCG CTCCGAGGAG GTCCAGGGGG CCCAGGAGGT 
CCTGGAGGAC CCATGGGGCG CATGGGAGGC CGCGGAGGAG ACAGAGGCGG CTTCCCACCA 
AGAGGGCCTC GCGGTTCCCG GGGGAACCCG TCTGGAGGAG GAAACGTCCA GCACCGAGCT 
GGAGACTGGC AGTGCCCCAA CCCGGGCTGT GGGAACCAGA ACTTCGCCTG GAGAACAGAG 
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CACCGCCTTT CCCGCCCCCA 
GGCGGTGACC GTGGCAGAGG TGGCCCTGGA GGCATGCGGG GAGGAAGAGG TGGCCTCATG 
GACCGCGGTG GTCCTGGTGG AATGTTCAGA GGCGGTCGTG GTGGAGACAG AGGTGGCTTC 
CGTGGCGGCC GGGGCATGGA CCGCGGTGGC TTTGGTGGTG GAAGACGAGG TGGCCCTGGG 
GGACCCCCTG GACCACTGAT GGAACAGATG GGAGGACGAC GAGGCGGGCG AGGAGGACCT 
GGAAAGATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ACCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218132.1
CDS	112..2085
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVSAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLVDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPATAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_005218075.3
Organism	Bos taurus(cattle)
Definition	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218075.3

ORF Insert Sequence:

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC 
ATGGATGAAG GACCAGACCT TGATTTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC 
AACAGTGCCA TTTATGTGCA AGGCCTGAAC GACAGTGTGA CTCTCGATGA CCTGGTGGAC 
TTCTTTAAGC AGTGTGGAGT CGTTAAGATG AACAAGAGGA CTGGACAACC CATGATCCAT 
ATTTACTTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CTACGGTGTC TTACGAAGAC 
CCAGCAACTG CCAAAGCTGC TGTCGAGTGG TTTGATGGGA AAGATTTCCA AGGGAGCAAA 
CTTAAAGTGT CTCTTGCTCG GAAGAAGCCG CCCATGAACA GCATGCGGGG AGGGATGCCG 
CCGCGCGAGG GCAGGGGGAT GCCGCCGCCG CTCCGAGGAG GTCCAGGGGG CCCAGGAGGT 
CCTGGAGGAC CCATGGGGCG CATGGGAGGC CGCGGAGGAG ACAGAGGCGG CTTCCCACCA 
AGAGGGCCTC GCGGTTCCCG GGGGAACCCG TCTGGAGGAG GAAACGTCCA GCACCGAGCT 
GGAGACTGGC AGTGCCCCAA CCCGGGCTGT GGGAACCAGA ACTTCGCCTG GAGAACAGAG 
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CACCGCCTTT CCCGCCCCCA 
GGCGGTGACC GTGGCAGAGG TGGCCCTGGA GGCATGCGGG GAGGAAGAGG TGGCCTCATG 
GACCGCGGTG GTCCTGGTGG AATGTTCAGA GGCGGTCGTG GTGGAGACAG AGGTGGCTTC 
CGTGGCGGCC GGGGCATGGA CCGCGGTGGC TTTGGTGGTG GAAGACGAGG TGGCCCTGGG 
GGACCCCCTG GACCACTGAT GGAACAGATG GGAGGACGAC GAGGCGGGCG AGGAGGACCT 
GGAAAGATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ACCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EWSR1 ( XM_005218075.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218132.1 Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28036
Clone ID Related Accession (Same CDS sequence)	XM_005218075.3 , XM_005218075.4
Accession Version	XM_005218075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RNA-binding protein EWS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218075.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC 
ATGGATGAAG GACCAGACCT TGATTTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC 
AACAGTGCCA TTTATGTGCA AGGCCTGAAC GACAGTGTGA CTCTCGATGA CCTGGTGGAC 
TTCTTTAAGC AGTGTGGAGT CGTTAAGATG AACAAGAGGA CTGGACAACC CATGATCCAT 
ATTTACTTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CTACGGTGTC TTACGAAGAC 
CCAGCAACTG CCAAAGCTGC TGTCGAGTGG TTTGATGGGA AAGATTTCCA AGGGAGCAAA 
CTTAAAGTGT CTCTTGCTCG GAAGAAGCCG CCCATGAACA GCATGCGGGG AGGGATGCCG 
CCGCGCGAGG GCAGGGGGAT GCCGCCGCCG CTCCGAGGAG GTCCAGGGGG CCCAGGAGGT 
CCTGGAGGAC CCATGGGGCG CATGGGAGGC CGCGGAGGAG ACAGAGGCGG CTTCCCACCA 
AGAGGGCCTC GCGGTTCCCG GGGGAACCCG TCTGGAGGAG GAAACGTCCA GCACCGAGCT 
GGAGACTGGC AGTGCCCCAA CCCGGGCTGT GGGAACCAGA ACTTCGCCTG GAGAACAGAG 
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CACCGCCTTT CCCGCCCCCA 
GGCGGTGACC GTGGCAGAGG TGGCCCTGGA GGCATGCGGG GAGGAAGAGG TGGCCTCATG 
GACCGCGGTG GTCCTGGTGG AATGTTCAGA GGCGGTCGTG GTGGAGACAG AGGTGGCTTC 
CGTGGCGGCC GGGGCATGGA CCGCGGTGGC TTTGGTGGTG GAAGACGAGG TGGCCCTGGG 
GGACCCCCTG GACCACTGAT GGAACAGATG GGAGGACGAC GAGGCGGGCG AGGAGGACCT 
GGAAAGATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ACCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218132.1
CDS	99..2072
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVSAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLVDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPATAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_005218075.4
Organism	Bos taurus(cattle)
Definition	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218075.4

ORF Insert Sequence:

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC 
ATGGATGAAG GACCAGACCT TGATTTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC 
AACAGTGCCA TTTATGTGCA AGGCCTGAAC GACAGTGTGA CTCTCGATGA CCTGGTGGAC 
TTCTTTAAGC AGTGTGGAGT CGTTAAGATG AACAAGAGGA CTGGACAACC CATGATCCAT 
ATTTACTTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CTACGGTGTC TTACGAAGAC 
CCAGCAACTG CCAAAGCTGC TGTCGAGTGG TTTGATGGGA AAGATTTCCA AGGGAGCAAA 
CTTAAAGTGT CTCTTGCTCG GAAGAAGCCG CCCATGAACA GCATGCGGGG AGGGATGCCG 
CCGCGCGAGG GCAGGGGGAT GCCGCCGCCG CTCCGAGGAG GTCCAGGGGG CCCAGGAGGT 
CCTGGAGGAC CCATGGGGCG CATGGGAGGC CGCGGAGGAG ACAGAGGCGG CTTCCCACCA 
AGAGGGCCTC GCGGTTCCCG GGGGAACCCG TCTGGAGGAG GAAACGTCCA GCACCGAGCT 
GGAGACTGGC AGTGCCCCAA CCCGGGCTGT GGGAACCAGA ACTTCGCCTG GAGAACAGAG 
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CACCGCCTTT CCCGCCCCCA 
GGCGGTGACC GTGGCAGAGG TGGCCCTGGA GGCATGCGGG GAGGAAGAGG TGGCCTCATG 
GACCGCGGTG GTCCTGGTGG AATGTTCAGA GGCGGTCGTG GTGGAGACAG AGGTGGCTTC 
CGTGGCGGCC GGGGCATGGA CCGCGGTGGC TTTGGTGGTG GAAGACGAGG TGGCCCTGGG 
GGACCCCCTG GACCACTGAT GGAACAGATG GGAGGACGAC GAGGCGGGCG AGGAGGACCT 
GGAAAGATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ACCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EWSR1 ( XM_005218076.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218133.1 Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa28037
Clone ID Related Accession (Same CDS sequence)	XM_005218076.3 , XM_005218076.4
Accession Version	XM_005218076.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	RNA-binding protein EWS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGGCATATGG GCAGCAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGATA TTCTACTCCA 
GCTGCCCCCC AGGCATACAG TCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGACACC 
ACCACTGCTA CGGTTACTAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT 
CAGCCCGCTT ACCCGGCGTA TGGACAGCAG CCAGCAGCCA CCGCGCCTGC AAGACCGCAG 
GATGGTAACA AACCCGCTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG 
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTGCCTGG GAGCTACCCC 
ATGCAGCCGG TCTCAGCACC ACCATCCTAT CCTCCTACCA GCTACTCCTC TACGCAGCCG 
ACTAGTTATG ATCAGAGCAG TTACTCCCAG CAGAACACCT ACGGGCAGCC GAGCAGCTAT 
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCACC CACTAGTTAC 
CCCCCCCAGA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGTCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218133.1
CDS	112..2082
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVSAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLVDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPATAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_005218076.3
Organism	Bos taurus(cattle)
Definition	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218076.3

ORF Insert Sequence:

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGGCATATGG GCAGCAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGATA TTCTACTCCA 
GCTGCCCCCC AGGCATACAG TCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGACACC 
ACCACTGCTA CGGTTACTAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT 
CAGCCCGCTT ACCCGGCGTA TGGACAGCAG CCAGCAGCCA CCGCGCCTGC AAGACCGCAG 
GATGGTAACA AACCCGCTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG 
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTGCCTGG GAGCTACCCC 
ATGCAGCCGG TCTCAGCACC ACCATCCTAT CCTCCTACCA GCTACTCCTC TACGCAGCCG 
ACTAGTTATG ATCAGAGCAG TTACTCCCAG CAGAACACCT ACGGGCAGCC GAGCAGCTAT 
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCACC CACTAGTTAC 
CCCCCCCAGA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGTCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EWSR1 ( XM_005218076.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218133.1 Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa28037
Clone ID Related Accession (Same CDS sequence)	XM_005218076.3 , XM_005218076.4
Accession Version	XM_005218076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RNA-binding protein EWS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGGCATATGG GCAGCAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGATA TTCTACTCCA 
GCTGCCCCCC AGGCATACAG TCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGACACC 
ACCACTGCTA CGGTTACTAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT 
CAGCCCGCTT ACCCGGCGTA TGGACAGCAG CCAGCAGCCA CCGCGCCTGC AAGACCGCAG 
GATGGTAACA AACCCGCTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG 
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTGCCTGG GAGCTACCCC 
ATGCAGCCGG TCTCAGCACC ACCATCCTAT CCTCCTACCA GCTACTCCTC TACGCAGCCG 
ACTAGTTATG ATCAGAGCAG TTACTCCCAG CAGAACACCT ACGGGCAGCC GAGCAGCTAT 
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCACC CACTAGTTAC 
CCCCCCCAGA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGTCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218133.1
CDS	91..2061
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVSAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLVDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPATAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_005218076.4
Organism	Bos taurus(cattle)
Definition	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218076.4

ORF Insert Sequence:

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGGCATATGG GCAGCAAAGT 
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC 
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGATA TTCTACTCCA 
GCTGCCCCCC AGGCATACAG TCAGCCTGTC CAGGGGTACG GCACTGGTGC TTATGACACC 
ACCACTGCTA CGGTTACTAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT 
CAGCCCGCTT ACCCGGCGTA TGGACAGCAG CCAGCAGCCA CCGCGCCTGC AAGACCGCAG 
GATGGTAACA AACCCGCTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG 
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTGCCTGG GAGCTACCCC 
ATGCAGCCGG TCTCAGCACC ACCATCCTAT CCTCCTACCA GCTACTCCTC TACGCAGCCG 
ACTAGTTATG ATCAGAGCAG TTACTCCCAG CAGAACACCT ACGGGCAGCC GAGCAGCTAT 
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCACC CACTAGTTAC 
CCCCCCCAGA CTGGATCCTA CAGCCAGGCT CCAAGTCAAT ATAGTCAACA GAGCAGCAGC 
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG 
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG 
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA 
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EWSR1 ( XM_005218077.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218134.1 Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X3, mRNA.

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CloneID	OBa28038
Clone ID Related Accession (Same CDS sequence)	XM_005218077.3 , XM_005218077.4
Accession Version	XM_005218077.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	RNA-binding protein EWS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218134.1
CDS	112..2082
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVSAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLVDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPATAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_005218077.3
Organism	Bos taurus(cattle)
Definition	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218077.3

ORF Insert Sequence:

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EWSR1 ( XM_005218077.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218134.1 Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa28038
Clone ID Related Accession (Same CDS sequence)	XM_005218077.3 , XM_005218077.4
Accession Version	XM_005218077.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RNA-binding protein EWS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218077.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218134.1
CDS	99..2069
Translation	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPAAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPARPQDGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVSAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLVDFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPATAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Target ORF information:

RefSeq Version	XM_005218077.4
Organism	Bos taurus(cattle)
Definition	Bos taurus EWS RNA binding protein 1 (EWSR1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218077.4

ORF Insert Sequence:

ATGGCGTCCA CGGATTATAG TACATACAGC CAAGCTGCAG CCCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACTACCC AGCAGGCATA TGGGCAGCAA 
AGTTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA 
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG ATATTCTACT 
CCAGCTGCCC CCCAGGCATA CAGTCAGCCT GTCCAGGGGT ACGGCACTGG TGCTTATGAC 
ACCACCACTG CTACGGTTAC TACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC 
ACTCAGCCCG CTTACCCGGC GTATGGACAG CAGCCAGCAG CCACCGCGCC TGCAAGACCG 
CAGGATGGTA ACAAACCCGC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC 
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTGCC TGGGAGCTAC 
CCCATGCAGC CGGTCTCAGC ACCACCATCC TATCCTCCTA CCAGCTACTC CTCTACGCAG 
CCGACTAGTT ATGATCAGAG CAGTTACTCC CAGCAGAACA CCTACGGGCA GCCGAGCAGC 
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC ACCCACTAGT 
TACCCCCCCC AGACTGGATC CTACAGCCAG GCTCCAAGTC AATATAGTCA ACAGAGCAGC 
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG 
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC 
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA 
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG 
GATGAAGGAC CAGACCTTGA TTTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC 
AGTGCCATTT ATGTGCAAGG CCTGAACGAC AGTGTGACTC TCGATGACCT GGTGGACTTC 
TTTAAGCAGT GTGGAGTCGT TAAGATGAAC AAGAGGACTG GACAACCCAT GATCCATATT 
TACTTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCTA CGGTGTCTTA CGAAGACCCA 
GCAACTGCCA AAGCTGCTGT CGAGTGGTTT GATGGGAAAG ATTTCCAAGG GAGCAAACTT 
AAAGTGTCTC TTGCTCGGAA GAAGCCGCCC ATGAACAGCA TGCGGGGAGG GATGCCGCCG 
CGCGAGGGCA GGGGGATGCC GCCGCCGCTC CGAGGAGGTC CAGGGGGCCC AGGAGGTCCT 
GGAGGACCCA TGGGGCGCAT GGGAGGCCGC GGAGGAGACA GAGGCGGCTT CCCACCAAGA 
GGGCCTCGCG GTTCCCGGGG GAACCCGTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA 
GACTGGCAGT GCCCCAACCC GGGCTGTGGG AACCAGAACT TCGCCTGGAG AACAGAGTGC 
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCAC CGCCTTTCCC GCCCCCAGGC 
GGTGACCGTG GCAGAGGTGG CCCTGGAGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAC 
CGCGGTGGTC CTGGTGGAAT GTTCAGAGGC GGTCGTGGTG GAGACAGAGG TGGCTTCCGT 
GGCGGCCGGG GCATGGACCG CGGTGGCTTT GGTGGTGGAA GACGAGGTGG CCCTGGGGGA 
CCCCCTGGAC CACTGATGGA ACAGATGGGA GGACGACGAG GCGGGCGAGG AGGACCTGGA 
AAGATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGACC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of bovine transcripts preferentially expressed in testis and with a putative role in spermatogenesis.
Theriogenology76(6)991-8(2011 Oct)
Alves BC,Tobo PR,Rodrigues R,Ruiz JC,de Lima VF,Moreira-Filho CA

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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EWSR1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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