EDRF1 cDNA ORF clone, Bos taurus(cattle)

The following EDRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231039 XM_024985862.1
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa12241 XM_003584125.4
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12241 XM_003587917.5
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12242 XM_005225865.4
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12242 XM_005193098.3
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12243 XM_002698564.6
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12243 XM_864253.8
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12244 XM_005193100.3
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa12244 XM_005225867.4
Latest version!
Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa12241 XM_003587917.4 Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12243 XM_002698564.5 Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12242 XM_005225865.3 Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa12244 XM_005225867.3 Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231039
    Clone ID Related Accession (Same CDS sequence) XM_024985862.1
    Accession Version XM_024985862.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTATCCTT GATGAAGACA TAGTCCTTAA AACCAACAAG
    CAGGTTTACT CCCAGCTCCT GAGAGCCACT GCGAACAAAA ACGCCTCTCT CTTGGAAAGA
    GTCGACCTCC TCATCCATCT GCTGGACCAG CTGGCTTGTG GCAGAGGCAG CAGCGCCGTC
    GTCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841630.1
    CDS135..3743
    Translation

    Target ORF information:

    RefSeq Version XM_024985862.1
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTATCCTT GATGAAGACA TAGTCCTTAA AACCAACAAG
    CAGGTTTACT CCCAGCTCCT GAGAGCCACT GCGAACAAAA ACGCCTCTCT CTTGGAAAGA
    GTCGACCTCC TCATCCATCT GCTGGACCAG CTGGCTTGTG GCAGAGGCAG CAGCGCCGTC
    GTCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12241
    Clone ID Related Accession (Same CDS sequence) XM_003587917.4 , XM_003584125.4 , XM_003587917.5
    Accession Version XM_003587917.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587917.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTAGTA TCCTTGATGA AGACATAGTC CTTAAAACCA ACAAGCAGGT TTACTCCCAG
    CTCCTGAGAG CCACTGCGAA CAAAAACGCC TCTCTCTTGG AAAGAGTCGA CCTCCTCATC
    CATCTGCTGG ACCAGCTGGC TTGTGGCAGA GGCAGCAGCG CCGTCGTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587965.1
    CDS107..3820
    Translation

    Target ORF information:

    RefSeq Version XM_003587917.4
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587917.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTAGTA TCCTTGATGA AGACATAGTC CTTAAAACCA ACAAGCAGGT TTACTCCCAG
    CTCCTGAGAG CCACTGCGAA CAAAAACGCC TCTCTCTTGG AAAGAGTCGA CCTCCTCATC
    CATCTGCTGG ACCAGCTGGC TTGTGGCAGA GGCAGCAGCG CCGTCGTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12241
    Clone ID Related Accession (Same CDS sequence) XM_003587917.4 , XM_003584125.4 , XM_003587917.5
    Accession Version XM_003584125.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003584125.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTAGTA TCCTTGATGA AGACATAGTC CTTAAAACCA ACAAGCAGGT TTACTCCCAG
    CTCCTGAGAG CCACTGCGAA CAAAAACGCC TCTCTCTTGG AAAGAGTCGA CCTCCTCATC
    CATCTGCTGG ACCAGCTGGC TTGTGGCAGA GGCAGCAGCG CCGTCGTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003584173.1
    CDS107..3820
    Translation

    Target ORF information:

    RefSeq Version XM_003584125.4
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003584125.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTAGTA TCCTTGATGA AGACATAGTC CTTAAAACCA ACAAGCAGGT TTACTCCCAG
    CTCCTGAGAG CCACTGCGAA CAAAAACGCC TCTCTCTTGG AAAGAGTCGA CCTCCTCATC
    CATCTGCTGG ACCAGCTGGC TTGTGGCAGA GGCAGCAGCG CCGTCGTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12241
    Clone ID Related Accession (Same CDS sequence) XM_003587917.4 , XM_003584125.4 , XM_003587917.5
    Accession Version XM_003587917.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587917.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTAGTA TCCTTGATGA AGACATAGTC CTTAAAACCA ACAAGCAGGT TTACTCCCAG
    CTCCTGAGAG CCACTGCGAA CAAAAACGCC TCTCTCTTGG AAAGAGTCGA CCTCCTCATC
    CATCTGCTGG ACCAGCTGGC TTGTGGCAGA GGCAGCAGCG CCGTCGTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587965.1
    CDS135..3848
    Translation

    Target ORF information:

    RefSeq Version XM_003587917.5
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587917.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTAGTA TCCTTGATGA AGACATAGTC CTTAAAACCA ACAAGCAGGT TTACTCCCAG
    CTCCTGAGAG CCACTGCGAA CAAAAACGCC TCTCTCTTGG AAAGAGTCGA CCTCCTCATC
    CATCTGCTGG ACCAGCTGGC TTGTGGCAGA GGCAGCAGCG CCGTCGTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12242
    Clone ID Related Accession (Same CDS sequence) XM_005193098.3 , XM_005225865.4 , XM_005225865.3
    Accession Version XM_005193098.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTATCC TTGATGAAGA CATAGTCCTT AAAACCAACA AGCAGGTTTA CTCCCAGCTC
    CTGAGAGCCA CTGCGAACAA AAACGCCTCT CTCTTGGAAA GAGTCGACCT CCTCATCCAT
    CTGCTGGACC AGCTGGCTTG TGGCAGAGGC AGCAGCGCCG TCGTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193155.1
    CDS107..3817
    Translation

    Target ORF information:

    RefSeq Version XM_005193098.3
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193098.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTATCC TTGATGAAGA CATAGTCCTT AAAACCAACA AGCAGGTTTA CTCCCAGCTC
    CTGAGAGCCA CTGCGAACAA AAACGCCTCT CTCTTGGAAA GAGTCGACCT CCTCATCCAT
    CTGCTGGACC AGCTGGCTTG TGGCAGAGGC AGCAGCGCCG TCGTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12242
    Clone ID Related Accession (Same CDS sequence) XM_005193098.3 , XM_005225865.4 , XM_005225865.3
    Accession Version XM_005225865.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTATCC TTGATGAAGA CATAGTCCTT AAAACCAACA AGCAGGTTTA CTCCCAGCTC
    CTGAGAGCCA CTGCGAACAA AAACGCCTCT CTCTTGGAAA GAGTCGACCT CCTCATCCAT
    CTGCTGGACC AGCTGGCTTG TGGCAGAGGC AGCAGCGCCG TCGTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225922.1
    CDS135..3845
    Translation

    Target ORF information:

    RefSeq Version XM_005225865.4
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225865.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTATCC TTGATGAAGA CATAGTCCTT AAAACCAACA AGCAGGTTTA CTCCCAGCTC
    CTGAGAGCCA CTGCGAACAA AAACGCCTCT CTCTTGGAAA GAGTCGACCT CCTCATCCAT
    CTGCTGGACC AGCTGGCTTG TGGCAGAGGC AGCAGCGCCG TCGTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12242
    Clone ID Related Accession (Same CDS sequence) XM_005193098.3 , XM_005225865.4 , XM_005225865.3
    Accession Version XM_005225865.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTATCC TTGATGAAGA CATAGTCCTT AAAACCAACA AGCAGGTTTA CTCCCAGCTC
    CTGAGAGCCA CTGCGAACAA AAACGCCTCT CTCTTGGAAA GAGTCGACCT CCTCATCCAT
    CTGCTGGACC AGCTGGCTTG TGGCAGAGGC AGCAGCGCCG TCGTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225922.1
    CDS107..3817
    Translation

    Target ORF information:

    RefSeq Version XM_005225865.3
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225865.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGGTTGGTG ATGAGCAGCT TAGGAAGCAG CACCGGGTGC TGGCAGACCT TCATTACAGC
    AAAGCTGGAA AGCTTTTTCA GCTTCTGAAA GACGCTCCGT GTGAACTCCT TAGAGTACAG
    TTAGAAAGAG TAGCCTTTGC AGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGGAAA
    TTAAAAACGC TATCTGGGGC CCTTGATATA ATGGTGAGAA CTAAGCATGC GTTCCAGCTT
    ATCAGAAAGG AGCTTGTGGA GGAATTTGAA CAGCCTAAGA GTGAAGAGAC CCCTCCAGCC
    GCTGATTCTT CACCTAGTCT TAATCGAGAA GAAGTGACAA AACTGCTCAG TATATTTGAA
    TCTCGCTTGT CATTCCTTCT CCTTCAGTCC ATCAAGCTGC TATCTTCAAC TAAGAAGAAA
    ACAAGTATCC TTGATGAAGA CATAGTCCTT AAAACCAACA AGCAGGTTTA CTCCCAGCTC
    CTGAGAGCCA CTGCGAACAA AAACGCCTCT CTCTTGGAAA GAGTCGACCT CCTCATCCAT
    CTGCTGGACC AGCTGGCTTG TGGCAGAGGC AGCAGCGCCG TCGTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12243
    Clone ID Related Accession (Same CDS sequence) XM_864253.8 , XM_002698564.5 , XM_002698564.6
    Accession Version XM_864253.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_864253.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTAGTATC CTTGATGAAG ACATAGTCCT TAAAACCAAC
    AAGCAGGTTT ACTCCCAGCT CCTGAGAGCC ACTGCGAACA AAAACGCCTC TCTCTTGGAA
    AGAGTCGACC TCCTCATCCA TCTGCTGGAC CAGCTGGCTT GTGGCAGAGG CAGCAGCGCC
    GTCGTCCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_869346.3
    CDS107..3718
    Translation

    Target ORF information:

    RefSeq Version XM_864253.8
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_864253.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTAGTATC CTTGATGAAG ACATAGTCCT TAAAACCAAC
    AAGCAGGTTT ACTCCCAGCT CCTGAGAGCC ACTGCGAACA AAAACGCCTC TCTCTTGGAA
    AGAGTCGACC TCCTCATCCA TCTGCTGGAC CAGCTGGCTT GTGGCAGAGG CAGCAGCGCC
    GTCGTCCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12243
    Clone ID Related Accession (Same CDS sequence) XM_864253.8 , XM_002698564.5 , XM_002698564.6
    Accession Version XM_002698564.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002698564.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTAGTATC CTTGATGAAG ACATAGTCCT TAAAACCAAC
    AAGCAGGTTT ACTCCCAGCT CCTGAGAGCC ACTGCGAACA AAAACGCCTC TCTCTTGGAA
    AGAGTCGACC TCCTCATCCA TCTGCTGGAC CAGCTGGCTT GTGGCAGAGG CAGCAGCGCC
    GTCGTCCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002698610.1
    CDS107..3718
    Translation

    Target ORF information:

    RefSeq Version XM_002698564.5
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002698564.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTAGTATC CTTGATGAAG ACATAGTCCT TAAAACCAAC
    AAGCAGGTTT ACTCCCAGCT CCTGAGAGCC ACTGCGAACA AAAACGCCTC TCTCTTGGAA
    AGAGTCGACC TCCTCATCCA TCTGCTGGAC CAGCTGGCTT GTGGCAGAGG CAGCAGCGCC
    GTCGTCCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12243
    Clone ID Related Accession (Same CDS sequence) XM_864253.8 , XM_002698564.5 , XM_002698564.6
    Accession Version XM_002698564.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002698564.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTAGTATC CTTGATGAAG ACATAGTCCT TAAAACCAAC
    AAGCAGGTTT ACTCCCAGCT CCTGAGAGCC ACTGCGAACA AAAACGCCTC TCTCTTGGAA
    AGAGTCGACC TCCTCATCCA TCTGCTGGAC CAGCTGGCTT GTGGCAGAGG CAGCAGCGCC
    GTCGTCCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002698610.1
    CDS135..3746
    Translation

    Target ORF information:

    RefSeq Version XM_002698564.6
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002698564.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTTTGTGGAC GTTTGAAGAC
    ATACACATGT TAGTTGGCTC CAACATGCCT ATCTTTGGAG GCGGCCGATA TCCAGCAGTC
    AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TTACTGGAAT TGACTATTGG
    TTGGACAACC TGATATGCAA CGTGCCAGAG CTTGTCATGT GTTTTCATGT AAATGGAATT
    GTACAGAAGT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA CTCTAATTTT
    TCTACTAAAG TCATAAAAGA CATTGCACAA AATATTTTAT CATTTTTAAA ATCTAATTGT
    ACCAAGGAAG GACATACCTA TTGGCTATTT AAAGCAAGTG GCAGTGATAT AGTGAAGCTC
    TACGATCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG
    CCAGTGGCTA TTCTTTTATA TAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT
    AAGAAACACT ACGGGACAGT TCGAACCCTG CTTCTGAATT GTGTTAAATT GCTGGACAAA
    AGCAGACATC CTCAAATTAT TGCTTCAGCC AATTACATGC TTTCAGAACT TTTTCAATTG
    GATGAACCTA AAAAAGAAGA AAGTTCAGAA TCTCCATTAA ATGAGAATTC TGATGAAAGT
    TACAGTGAAG AGGAGGAGGA GATGCCAGAC AGCGATGAAA ACGGGTCATA CAGCACTAGC
    TCTGACCCAT CGGACGATAA CAAAGCAGTA GCTATAATTA AGTCTGTTGG AGAGCTGTCC
    GTTCCAGAAA AATACAAATC CATTCATCAA ATCAGGCCCA GTTGTGCGTT TCCGGTTTGT
    CATGACACAG AAGAGCGCTG TAGGCTTGTG CTGAGCTACG TTCTGGAGGG TTTAAAGTCT
    GTGGACAGCA GCATCAAAAA AGAAAGTGAT CTCCCTGCCG CTGACCCCAA CACCCCAATC
    CCATTAAAAT ATGAGGATGA ATCCACAAGA GGGGGTCCTG AGGGTCTAGA GAAGCAGATG
    GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGTA ATTATTCCAG TCAATCTGGA
    ATGATCCCTG GTTCTTGGCA ACATAAAATG AAGCTGCAGC TGATTCTCAA GTCATCAAAG
    GCCTATTACG TCTTGTCTGA TGCAGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA
    TACATTAAAT TAGCTTTGCA GAGCCATGAT ACTTACTGCT GCCTCTGCAC CAATATGCTT
    TCTGAAGTGC TGCTGTTTCT TTCTCAGTAT TTGACGCTCT GCGGTGATAT CCAGCTAATG
    CTGGCCCAGA ATGCAAGCAA CAGGGCGGCA CACCTTGAGG AGTTCAATTA CCAAACGAAG
    GAGGACCAGG AGATCCTGCA CAGCCTCCAC AGGGAGTCCA GCTGCCAGGG ATTTGCATGG
    GCCACTGATT TGTCTACAGA CTTAGAAAGT CAACTTTCAG TTAGTTGTAA ATGTTATGAG
    GCCGCTAATG AAATCTTGCA GTTTAGTGAC TTAAAAAGTC AAAATCCAGA ACACTATGTA
    CAAGTACTGA AGAGAATGGG TAACATTAGA AATGAAATCG GTGTCTTTTA CATGAATCAG
    GCTGCTGCGT TGCAGAGTGA GAGACTAGTG AGCAAAACTG TGTCCACTGC AGAGCAACAG
    TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA GTCAATCGAC
    GATGCCACAA ATGCGGCCCT TTTATTATGT AACACGGGAC GACTCATGCG GATTTGTGCA
    CAGGCGCACT GTGGCGCAGG TGATGAATTT AAACGCGAAT TTTCACCGGA AGAAGGCTTG
    TATTACAATA AGGCTGTTGA TTACTACCTG AAAGCTCTAA GGTCCTTGGG AACACGGGAC
    ATACACCCAG CCGTCTGGGA CTCGGTGAAT TGGGAACTGT CCACTACTTA TTTTACGATG
    GCAACTCTAC AGCAAGATTA TGCTCCATTA TCTAGAAAAG CTCAAGAGCA GATTGAAAAA
    GAAGTCAGCG AGGCGATGAT GAAGTCCCTG AAATACTGCG ACGTGGACTC GGTGTCTGCC
    CGACAGCCCC TCTGTCAGTA CCGAGCTGCA ACCATCCATC ACAGGTTGGC GTCCATGTAT
    CACAGCTGCC TGCGGAACCA GGTTGGTGAT GAGCAGCTTA GGAAGCAGCA CCGGGTGCTG
    GCAGACCTTC ATTACAGCAA AGCTGGAAAG CTTTTTCAGC TTCTGAAAGA CGCTCCGTGT
    GAACTCCTTA GAGTACAGTT AGAAAGAGTA GCCTTTGCAG AATTTCAGAT GACCAGTCAG
    AATAGCAATG TTGGGAAATT AAAAACGCTA TCTGGGGCCC TTGATATAAT GGTGAGAACT
    AAGCATGCGT TCCAGCTTAT CAGAAAGGAG CTTGTGGAGG AATTTGAACA GCCTAAGAGT
    GAAGAGACCC CTCCAGCCGC TGATTCTTCA CCTAGTCTTA ATCGAGAAGA AGTGACAAAA
    CTGCTCAGTA TATTTGAATC TCGCTTGTCA TTCCTTCTCC TTCAGTCCAT CAAGCTGCTA
    TCTTCAACTA AGAAGAAAAC AAGTAGTATC CTTGATGAAG ACATAGTCCT TAAAACCAAC
    AAGCAGGTTT ACTCCCAGCT CCTGAGAGCC ACTGCGAACA AAAACGCCTC TCTCTTGGAA
    AGAGTCGACC TCCTCATCCA TCTGCTGGAC CAGCTGGCTT GTGGCAGAGG CAGCAGCGCC
    GTCGTCCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12244
    Clone ID Related Accession (Same CDS sequence) XM_005193100.3 , XM_005225867.3 , XM_005225867.4
    Accession Version XM_005193100.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGATTTGTA TGCATTGGCT TAGTGTTGGA TTGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193157.1
    CDS107..3265
    Translation

    Target ORF information:

    RefSeq Version XM_005193100.3
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193100.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGATTTGTA TGCATTGGCT TAGTGTTGGA TTGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12244
    Clone ID Related Accession (Same CDS sequence) XM_005193100.3 , XM_005225867.3 , XM_005225867.4
    Accession Version XM_005225867.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGATTTGTA TGCATTGGCT TAGTGTTGGA TTGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225924.1
    CDS107..3265
    Translation

    Target ORF information:

    RefSeq Version XM_005225867.3
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGATTTGTA TGCATTGGCT TAGTGTTGGA TTGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12244
    Clone ID Related Accession (Same CDS sequence) XM_005193100.3 , XM_005225867.3 , XM_005225867.4
    Accession Version XM_005225867.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product erythroid differentiation-related factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGATTTGTA TGCATTGGCT TAGTGTTGGA TTGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225924.1
    CDS136..3294
    Translation

    Target ORF information:

    RefSeq Version XM_005225867.4
    Organism Bos taurus(cattle)
    Definition Bos taurus erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225867.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGACT CTAAGGAGGC CGGGGCCGAG GCTCCGCCGG CCGGGGCCGC TGCTCGGGGA 
    GGGCTCAGCC TCCTGTCCCA AGGGGAGTCG GAGGAACCTT CTGCACAGGG ATCAGCTTTA
    TTTCTCGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT
    CGGACAGCAT TTGCACGCCT CGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG
    TTACGAGAGA GTGCCAAGCT AGGACCGGCA GGAACTACCA TTCTTGGCAA TAGTAGGAAA
    AGCAAGCCAT TTTCCAGTTT TGGGATGGCA TACGACTTTA TTGATTCAGT GGGAAACGAT
    GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAGCTTC TGAAGATTCC CTACAGCAAA
    TCACACGTGA GCATGGCTGT GCACCGCATA GGAAGGACTC TCTTGTTAGA CGAGTTAGAT
    ATCCAGGAAC TCTTTATGAG ATCATCTCAG ACTGGTGATT GGACATGGTT AAAAGAGTTT
    TATCAAAGAC TTATTGATCA GAAATGGCAG AGGAAGAAAA AAAGTAAAGA ACACTGGTAT
    CAGAAGGCTA TTCTTTCGAA GTTTTTGTAT TACAGTATCA ATGGCGATGG AGCTGCTCAG
    CCTGTCCCGT CTGCCACAGA ACAGCAGGAG TCGTCCAGCT CTGATCAGAC CAATGATTCA
    GATGGGGCTT CATGGCCTGC CCCCTTTGAA ATGCCTTCTT CAGTCTCTGA AGATCCCAGT
    GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA
    GCGCCTAAAG AACAAAACCT GACTCCTTTA TTCAATGACG GGGAGAACAG TCAGGGTCTT
    AAAAATGATT TTGTTCGGAA TATTTTGTGG ACGTTTGAAG ACATACACAT GTTAGTTGGC
    TCCAACATGC CTATCTTTGG AGGCGGCCGA TATCCAGCAG TCAGCTTACG TCTCAGGGAT
    AACAACAAAC CAATTAATGT GCTTACTGGA ATTGACTATT GGTTGGACAA CCTGATATGC
    AACGTGCCAG AGCTTGTCAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA GTATGAAATG
    ATAAAGACAG AAGAAATTCC CAATTTGGAA AACTCTAATT TTTCTACTAA AGTCATAAAA
    GACATTGCAC AAAATATTTT ATCATTTTTA AAATCTAATT GTACCAAGGA AGGACATACC
    TATTGGCTAT TTAAAGCAAG TGGCAGTGAT ATAGTGAAGC TCTACGATCT CACTACTCTT
    TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCAGTGGC TATTCTTTTA
    TATAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTACGGGACA
    GTTCGAACCC TGCTTCTGAA TTGTGTTAAA TTGCTGGACA AAAGCAGACA TCCTCAAATT
    ATTGCTTCAG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAAGAA
    GAAAGTTCAG AATCTCCATT AAATGAGAAT TCTGATGAAA GTTACAGTGA AGAGGAGGAG
    GAGATGCCAG ACAGCGATGA AAACGGGTCA TACAGCACTA GCTCTGACCC ATCGGACGAT
    AACAAAGCAG TAGCTATAAT TAAGTCTGTT GGAGAGCTGT CCGTTCCAGA AAAATACAAA
    TCCATTCATC AAATCAGGCC CAGTTGTGCG TTTCCGGTTT GTCATGACAC AGAAGAGCGC
    TGTAGGCTTG TGCTGAGCTA CGTTCTGGAG GGTTTAAAGT CTGTGGACAG CAGCATCAAA
    AAAGAAAGTG ATCTCCCTGC CGCTGACCCC AACACCCCAA TCCCATTAAA ATATGAGGAT
    GAATCCACAA GAGGGGGTCC TGAGGGTCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA
    ATGGGCTCCC TTCAGAAGGG TAATTATTCC AGTCAATCTG GAATGATCCC TGGTTCTTGG
    CAACATAAAA TGAAGCTGCA GCTGATTCTC AAGTCATCAA AGGCCTATTA CGTCTTGTCT
    GATGCAGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG
    CAGAGCCATG ATACTTACTG CTGCCTCTGC ACCAATATGC TTTCTGAAGT GCTGCTGTTT
    CTTTCTCAGT ATTTGACGCT CTGCGGTGAT ATCCAGCTAA TGCTGGCCCA GAATGCAAGC
    AACAGGGCGG CACACCTTGA GGAGTTCAAT TACCAAACGA AGGAGGACCA GGAGATCCTG
    CACAGCCTCC ACAGGGAGTC CAGCTGCCAG GGATTTGCAT GGGCCACTGA TTTGTCTACA
    GACTTAGAAA GTCAACTTTC AGTTAGTTGT AAATGTTATG AGGCCGCTAA TGAAATCTTG
    CAGTTTAGTG ACTTAAAAAG TCAAAATCCA GAACACTATG TACAAGTACT GAAGAGAATG
    GGTAACATTA GAAATGAAAT CGGTGTCTTT TACATGAATC AGGCTGCTGC GTTGCAGAGT
    GAGAGACTAG TGAGCAAAAC TGTGTCCACT GCAGAGCAAC AGTTGTGGAA AAAAAGCTTT
    TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAGTCAATCG ACGATGCCAC AAATGCGGCC
    CTTTTATTAT GTAACACGGG ACGACTCATG CGGATTTGTG CACAGGCGCA CTGTGGCGCA
    GGTGATGAAT TTAAACGCGA ATTTTCACCG GAAGAAGGCT TGTATTACAA TAAGGCTGTT
    GATTACTACC TGAAAGCTCT AAGGTCCTTG GGAACACGGG ACATACACCC AGCCGTCTGG
    GACTCGGTGA ATTGGGAACT GTCCACTACT TATTTTACGA TGGCAACTCT ACAGCAAGAT
    TATGCTCCAT TATCTAGAAA AGCTCAAGAG CAGATTGAAA AAGAAGTCAG CGAGGCGATG
    ATGAAGTCCC TGAAATACTG CGACGTGGAC TCGGTGTCTG CCCGACAGCC CCTCTGTCAG
    TACCGAGCTG CAACCATCCA TCACAGGTTG GCGTCCATGT ATCACAGCTG CCTGCGGAAC
    CAGATTTGTA TGCATTGGCT TAGTGTTGGA TTGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.