MICAL2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MICAL2
Entrez Gene ID	534041
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 2
Synonyms	MICAL-2
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 2 Names [F-actin]-monooxygenase MICAL2 protein-methionine sulfoxide oxidase MICAL2 MICAL C-terminal-like protein [F-actin]-methionine sulfoxide oxidase MICAL2 microtubule associated monoxygenase, calponin and LIM domain containing 2 molecule interacting with CasL protein 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015466513.1	XP_015321999.1	protein-methionine sulfoxide oxidase MICAL2 isoform X15
XM_010822805.2	XP_010821107.2	protein-methionine sulfoxide oxidase MICAL2 isoform X2
XM_015466502.1	XP_015321988.1	protein-methionine sulfoxide oxidase MICAL2 isoform X5
XM_015466507.1	XP_015321993.1	protein-methionine sulfoxide oxidase MICAL2 isoform X10
XM_010812539.2	XP_010810841.1	protein-methionine sulfoxide oxidase MICAL2 isoform X4
XM_002693051.5	XP_002693097.1	protein-methionine sulfoxide oxidase MICAL2 isoform X11
XM_010812544.3	XP_010810846.2	F-actin-monooxygenase MICAL2 isoform X5
XM_010812546.3	XP_010810848.1	F-actin-monooxygenase MICAL2 isoform X8
XM_005216093.4	XP_005216150.1	F-actin-monooxygenase MICAL2 isoform X9
XM_015474699.1	XP_015330185.1	protein-methionine sulfoxide oxidase MICAL2 isoform X5
XM_010812547.2	XP_010810849.1	protein-methionine sulfoxide oxidase MICAL2 isoform X15
XM_015466509.1	XP_015321995.1	protein-methionine sulfoxide oxidase MICAL2 isoform X11
XM_015466510.1	XP_015321996.1	protein-methionine sulfoxide oxidase MICAL2 isoform X12
XM_010812542.3	XP_010810844.2	F-actin-monooxygenase MICAL2 isoform X3
XM_010812543.3	XP_010810845.2	F-actin-monooxygenase MICAL2 isoform X4
XM_010812545.3	XP_010810847.1	F-actin-monooxygenase MICAL2 isoform X6
XM_010812538.2	XP_010810840.1	protein-methionine sulfoxide oxidase MICAL2 isoform X2
XM_005216092.3	XP_005216149.1	protein-methionine sulfoxide oxidase MICAL2 isoform X12
XM_010812546.2	XP_010810848.1	protein-methionine sulfoxide oxidase MICAL2 isoform X13
XM_010812541.2	XP_010810843.1	protein-methionine sulfoxide oxidase MICAL2 isoform X7
XM_010812542.2	XP_010810844.1	protein-methionine sulfoxide oxidase MICAL2 isoform X8
XM_015466498.1	XP_015321984.1	protein-methionine sulfoxide oxidase MICAL2 isoform X1
XM_015466499.1	XP_015321985.1	protein-methionine sulfoxide oxidase MICAL2 isoform X1
XM_015466503.1	XP_015321989.1	protein-methionine sulfoxide oxidase MICAL2 isoform X6
XM_015466504.1	XP_015321990.1	protein-methionine sulfoxide oxidase MICAL2 isoform X7
XM_010812538.3	XP_010810840.2	F-actin-monooxygenase MICAL2 isoform X1
XM_024975804.1	XP_024831572.1	F-actin-monooxygenase MICAL2 isoform X7
XM_015466500.1	XP_015321986.1	protein-methionine sulfoxide oxidase MICAL2 isoform X3
XM_015466505.1	XP_015321991.1	protein-methionine sulfoxide oxidase MICAL2 isoform X8
XM_015466508.1	XP_015321994.1	protein-methionine sulfoxide oxidase MICAL2 isoform X11
XM_010812537.2	XP_010810839.1	protein-methionine sulfoxide oxidase MICAL2 isoform X1
XM_015474698.1	XP_015330184.1	protein-methionine sulfoxide oxidase MICAL2 isoform X3
XM_010812540.2	XP_010810842.1	protein-methionine sulfoxide oxidase MICAL2 isoform X6
XM_010812543.2	XP_010810845.1	protein-methionine sulfoxide oxidase MICAL2 isoform X9
XM_010812544.2	XP_010810846.1	protein-methionine sulfoxide oxidase MICAL2 isoform X10
XM_010812541.3	XP_010810843.2	F-actin-monooxygenase MICAL2 isoform X2
XM_002693051.6	XP_002693097.1	F-actin-monooxygenase MICAL2 isoform X6
XM_015466511.1	XP_015321997.1	protein-methionine sulfoxide oxidase MICAL2 isoform X13
XM_015474697.1	XP_015330183.1	protein-methionine sulfoxide oxidase MICAL2 isoform X1
XM_010812545.2	XP_010810847.1	protein-methionine sulfoxide oxidase MICAL2 isoform X11
XM_005216093.3	XP_005216150.1	protein-methionine sulfoxide oxidase MICAL2 isoform X14
XM_015466501.1	XP_015321987.1	protein-methionine sulfoxide oxidase MICAL2 isoform X4
XM_015466506.1	XP_015321992.1	protein-methionine sulfoxide oxidase MICAL2 isoform X9
XM_015466512.1	XP_015321998.1	protein-methionine sulfoxide oxidase MICAL2 isoform X14
XM_005216092.4	XP_005216149.1	F-actin-monooxygenase MICAL2 isoform X7
XM_010812547.3	XP_010810849.1	F-actin-monooxygenase MICAL2 isoform X10

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MICAL2	XP_005633730.1
Bos taurus (cattle)	MICAL2	XP_002693097.1
Pan troglodytes (chimpanzee)	MICAL2	XP_003951886.1
Mus musculus (house mouse)	Mical2	NP_001180234.1
Rattus norvegicus (Norway rat)	Mical2	NP_001132980.1
Gallus gallus (chicken)	MICAL2	XP_003641364.2
Xenopus tropicalis (tropical clawed frog)	LOC100487018	XP_002941765.2
Homo sapiens (human)	MICAL2	NP_055447.1
Danio rerio (zebrafish)	mical2b	NP_001258842.1

Gene Ontology

The following MICAL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MICAL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa08131	XM_015466512.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa08131	XM_005216093.4 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa52590	XM_015466511.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa52590	XM_010812546.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa52591	XM_015466513.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa52591	XM_010812547.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa08129	XM_015466509.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08129	XM_010812545.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08129	XM_015466508.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08129	XM_002693051.6 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08130	XM_024975804.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08130	XM_005216092.4 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08130	XM_015466510.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa223227	XM_010812538.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223228	XM_010812541.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223229	XM_010812542.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223230	XM_010812543.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223231	XM_010812544.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa92667	XM_015474698.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92667	XM_015466500.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92668	XM_015466502.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92668	XM_015474699.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa52582	XM_015474697.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52582	XM_015466499.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52582	XM_015466498.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52582	XM_010812537.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52583	XM_010822805.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52583	XM_010812538.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52584	XM_010812539.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52584	XM_015466501.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52585	XM_015466503.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52585	XM_010812540.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52586	XM_015466504.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52586	XM_010812541.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52587	XM_015466505.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52587	XM_010812542.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52588	XM_015466506.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52588	XM_010812543.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52589	XM_015466507.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52589	XM_010812544.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OBa52590	XM_010812546.2	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa08129	XM_002693051.5	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08129	XM_010812545.2	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa08131	XM_005216093.3	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa08130	XM_005216092.3	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa52591	XM_010812547.2	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MICAL2 ( XM_005216093.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216150.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.

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CloneID	OBa08131
Clone ID Related Accession (Same CDS sequence)	XM_005216093.4 , XM_005216093.3 , XM_015466512.1
Accession Version	XM_005216093.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216093.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGGTA TCCCCAATTC CTTCTTTAGG AAGGCACTAA GCTGGCCCCT CAGGCTGCCG 
TGGGGCCTGC TTCACCTTCC CCGGAGGCTG ATTAACTGGA TTCAGGGACT CCTACGCGCC 
ACCAGCCTGC ATATCAGGGA CAATGCTTAC AACTACTGCT ACACCTACGA GCTCCTGAGC 
CTCGGGCTGC CCCTGCTCTG GGTGTTCTCC GAGGTCCTGG CTTCCATGTA CCGGGAGTCG 
GAGGAGTCCC TCCAGAGCAT CCGCGACTGG CTGCTCAGAC GCGTCCCGGC CAAGCTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216150.1
CDS	312..3194
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPGIPNSFFRKALSWPLRLPWGLLHLPRRLINWIQGLLRATSLHIRDNAYNYCYTYELLSLGLPLLWVFSEVLASMYRESEESLQSIRDWLLRRVPAKLH

Target ORF information:

RefSeq Version	XM_005216093.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216093.4

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGGTA TCCCCAATTC CTTCTTTAGG AAGGCACTAA GCTGGCCCCT CAGGCTGCCG 
TGGGGCCTGC TTCACCTTCC CCGGAGGCTG ATTAACTGGA TTCAGGGACT CCTACGCGCC 
ACCAGCCTGC ATATCAGGGA CAATGCTTAC AACTACTGCT ACACCTACGA GCTCCTGAGC 
CTCGGGCTGC CCCTGCTCTG GGTGTTCTCC GAGGTCCTGG CTTCCATGTA CCGGGAGTCG 
GAGGAGTCCC TCCAGAGCAT CCGCGACTGG CTGCTCAGAC GCGTCCCGGC CAAGCTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_005216093.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216150.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa08131
Clone ID Related Accession (Same CDS sequence)	XM_005216093.4 , XM_005216093.3 , XM_015466512.1
Accession Version	XM_005216093.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGGTA TCCCCAATTC CTTCTTTAGG AAGGCACTAA GCTGGCCCCT CAGGCTGCCG 
TGGGGCCTGC TTCACCTTCC CCGGAGGCTG ATTAACTGGA TTCAGGGACT CCTACGCGCC 
ACCAGCCTGC ATATCAGGGA CAATGCTTAC AACTACTGCT ACACCTACGA GCTCCTGAGC 
CTCGGGCTGC CCCTGCTCTG GGTGTTCTCC GAGGTCCTGG CTTCCATGTA CCGGGAGTCG 
GAGGAGTCCC TCCAGAGCAT CCGCGACTGG CTGCTCAGAC GCGTCCCGGC CAAGCTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216150.1
CDS	324..3206
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPGIPNSFFRKALSWPLRLPWGLLHLPRRLINWIQGLLRATSLHIRDNAYNYCYTYELLSLGLPLLWVFSEVLASMYRESEESLQSIRDWLLRRVPAKLH

Target ORF information:

RefSeq Version	XM_005216093.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216093.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGGTA TCCCCAATTC CTTCTTTAGG AAGGCACTAA GCTGGCCCCT CAGGCTGCCG 
TGGGGCCTGC TTCACCTTCC CCGGAGGCTG ATTAACTGGA TTCAGGGACT CCTACGCGCC 
ACCAGCCTGC ATATCAGGGA CAATGCTTAC AACTACTGCT ACACCTACGA GCTCCTGAGC 
CTCGGGCTGC CCCTGCTCTG GGTGTTCTCC GAGGTCCTGG CTTCCATGTA CCGGGAGTCG 
GAGGAGTCCC TCCAGAGCAT CCGCGACTGG CTGCTCAGAC GCGTCCCGGC CAAGCTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466512.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321998.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa08131
Clone ID Related Accession (Same CDS sequence)	XM_005216093.4 , XM_005216093.3 , XM_015466512.1
Accession Version	XM_015466512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGGTA TCCCCAATTC CTTCTTTAGG AAGGCACTAA GCTGGCCCCT CAGGCTGCCG 
TGGGGCCTGC TTCACCTTCC CCGGAGGCTG ATTAACTGGA TTCAGGGACT CCTACGCGCC 
ACCAGCCTGC ATATCAGGGA CAATGCTTAC AACTACTGCT ACACCTACGA GCTCCTGAGC 
CTCGGGCTGC CCCTGCTCTG GGTGTTCTCC GAGGTCCTGG CTTCCATGTA CCGGGAGTCG 
GAGGAGTCCC TCCAGAGCAT CCGCGACTGG CTGCTCAGAC GCGTCCCGGC CAAGCTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321998.1
CDS	324..3206
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPGIPNSFFRKALSWPLRLPWGLLHLPRRLINWIQGLLRATSLHIRDNAYNYCYTYELLSLGLPLLWVFSEVLASMYRESEESLQSIRDWLLRRVPAKLH

Target ORF information:

RefSeq Version	XM_015466512.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466512.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGGTA TCCCCAATTC CTTCTTTAGG AAGGCACTAA GCTGGCCCCT CAGGCTGCCG 
TGGGGCCTGC TTCACCTTCC CCGGAGGCTG ATTAACTGGA TTCAGGGACT CCTACGCGCC 
ACCAGCCTGC ATATCAGGGA CAATGCTTAC AACTACTGCT ACACCTACGA GCTCCTGAGC 
CTCGGGCTGC CCCTGCTCTG GGTGTTCTCC GAGGTCCTGG CTTCCATGTA CCGGGAGTCG 
GAGGAGTCCC TCCAGAGCAT CCGCGACTGG CTGCTCAGAC GCGTCCCGGC CAAGCTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812546.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810848.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa52590
Clone ID Related Accession (Same CDS sequence)	XM_010812546.3 , XM_010812546.2 , XM_015466511.1
Accession Version	XM_010812546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG GTATCCCCAA TTCCTTCTTT AGGAAGGCAC TAAGCTGGCC CCTCAGGCTG 
CCGTGGGGCC TGCTTCACCT TCCCCGGAGG CTGATTAACT GGATTCAGGG ACTCCTACGC 
GCCACCAGCC TGCATATCAG GGACAATGCT TACAACTACT GCTACACCTA CGAGCTCCTG 
AGCCTCGGGC TGCCCCTGCT CTGGGTGTTC TCCGAGGTCC TGGCTTCCAT GTACCGGGAG 
TCGGAGGAGT CCCTCCAGAG CATCCGCGAC TGGCTGCTCA GACGCGTCCC GGCCAAGCTG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810848.1
CDS	306..3251
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPGIPNSFFRKALSWPLRLPWGLLHLPRRLINWIQGLLRATSLHIRDNAYNYCYTYELLSLGLPLLWVFSEVLASMYRESEESLQSIRDWLLRRVPAKLH

Target ORF information:

RefSeq Version	XM_010812546.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812546.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG GTATCCCCAA TTCCTTCTTT AGGAAGGCAC TAAGCTGGCC CCTCAGGCTG 
CCGTGGGGCC TGCTTCACCT TCCCCGGAGG CTGATTAACT GGATTCAGGG ACTCCTACGC 
GCCACCAGCC TGCATATCAG GGACAATGCT TACAACTACT GCTACACCTA CGAGCTCCTG 
AGCCTCGGGC TGCCCCTGCT CTGGGTGTTC TCCGAGGTCC TGGCTTCCAT GTACCGGGAG 
TCGGAGGAGT CCCTCCAGAG CATCCGCGAC TGGCTGCTCA GACGCGTCCC GGCCAAGCTG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812546.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810848.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa52590
Clone ID Related Accession (Same CDS sequence)	XM_010812546.3 , XM_010812546.2 , XM_015466511.1
Accession Version	XM_010812546.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG GTATCCCCAA TTCCTTCTTT AGGAAGGCAC TAAGCTGGCC CCTCAGGCTG 
CCGTGGGGCC TGCTTCACCT TCCCCGGAGG CTGATTAACT GGATTCAGGG ACTCCTACGC 
GCCACCAGCC TGCATATCAG GGACAATGCT TACAACTACT GCTACACCTA CGAGCTCCTG 
AGCCTCGGGC TGCCCCTGCT CTGGGTGTTC TCCGAGGTCC TGGCTTCCAT GTACCGGGAG 
TCGGAGGAGT CCCTCCAGAG CATCCGCGAC TGGCTGCTCA GACGCGTCCC GGCCAAGCTG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810848.1
CDS	324..3269
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPGIPNSFFRKALSWPLRLPWGLLHLPRRLINWIQGLLRATSLHIRDNAYNYCYTYELLSLGLPLLWVFSEVLASMYRESEESLQSIRDWLLRRVPAKLH

Target ORF information:

RefSeq Version	XM_010812546.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812546.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG GTATCCCCAA TTCCTTCTTT AGGAAGGCAC TAAGCTGGCC CCTCAGGCTG 
CCGTGGGGCC TGCTTCACCT TCCCCGGAGG CTGATTAACT GGATTCAGGG ACTCCTACGC 
GCCACCAGCC TGCATATCAG GGACAATGCT TACAACTACT GCTACACCTA CGAGCTCCTG 
AGCCTCGGGC TGCCCCTGCT CTGGGTGTTC TCCGAGGTCC TGGCTTCCAT GTACCGGGAG 
TCGGAGGAGT CCCTCCAGAG CATCCGCGAC TGGCTGCTCA GACGCGTCCC GGCCAAGCTG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466511.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321997.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa52590
Clone ID Related Accession (Same CDS sequence)	XM_010812546.3 , XM_010812546.2 , XM_015466511.1
Accession Version	XM_015466511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG GTATCCCCAA TTCCTTCTTT AGGAAGGCAC TAAGCTGGCC CCTCAGGCTG 
CCGTGGGGCC TGCTTCACCT TCCCCGGAGG CTGATTAACT GGATTCAGGG ACTCCTACGC 
GCCACCAGCC TGCATATCAG GGACAATGCT TACAACTACT GCTACACCTA CGAGCTCCTG 
AGCCTCGGGC TGCCCCTGCT CTGGGTGTTC TCCGAGGTCC TGGCTTCCAT GTACCGGGAG 
TCGGAGGAGT CCCTCCAGAG CATCCGCGAC TGGCTGCTCA GACGCGTCCC GGCCAAGCTG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321997.1
CDS	324..3269
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPGIPNSFFRKALSWPLRLPWGLLHLPRRLINWIQGLLRATSLHIRDNAYNYCYTYELLSLGLPLLWVFSEVLASMYRESEESLQSIRDWLLRRVPAKLH

Target ORF information:

RefSeq Version	XM_015466511.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466511.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG GTATCCCCAA TTCCTTCTTT AGGAAGGCAC TAAGCTGGCC CCTCAGGCTG 
CCGTGGGGCC TGCTTCACCT TCCCCGGAGG CTGATTAACT GGATTCAGGG ACTCCTACGC 
GCCACCAGCC TGCATATCAG GGACAATGCT TACAACTACT GCTACACCTA CGAGCTCCTG 
AGCCTCGGGC TGCCCCTGCT CTGGGTGTTC TCCGAGGTCC TGGCTTCCAT GTACCGGGAG 
TCGGAGGAGT CCCTCCAGAG CATCCGCGAC TGGCTGCTCA GACGCGTCCC GGCCAAGCTG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466513.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321999.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa52591
Clone ID Related Accession (Same CDS sequence)	XM_015466513.1 , XM_010812547.2 , XM_010812547.3
Accession Version	XM_015466513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGTC 
CATTTCAGCC TTCCAGTGCT GCACCCACTT CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321999.1
CDS	324..3122
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_015466513.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466513.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGTC 
CATTTCAGCC TTCCAGTGCT GCACCCACTT CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812547.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810849.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa52591
Clone ID Related Accession (Same CDS sequence)	XM_015466513.1 , XM_010812547.2 , XM_010812547.3
Accession Version	XM_010812547.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGTC 
CATTTCAGCC TTCCAGTGCT GCACCCACTT CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810849.1
CDS	324..3122
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_010812547.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812547.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGTC 
CATTTCAGCC TTCCAGTGCT GCACCCACTT CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812547.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810849.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa52591
Clone ID Related Accession (Same CDS sequence)	XM_015466513.1 , XM_010812547.2 , XM_010812547.3
Accession Version	XM_010812547.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGTC 
CATTTCAGCC TTCCAGTGCT GCACCCACTT CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810849.1
CDS	305..3103
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_010812547.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812547.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGTC 
CATTTCAGCC TTCCAGTGCT GCACCCACTT CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_002693051.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002693097.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X13, mRNA.

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CloneID	OBa08129
Clone ID Related Accession (Same CDS sequence)	XM_002693051.5 , XM_015466509.1 , XM_010812545.3 , XM_015466508.1 , XM_002693051.6 , XM_010812545.2
Accession Version	XM_002693051.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002693051.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002693097.1
CDS	325..3693
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_002693051.5
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002693051.5

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466509.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321995.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X13, mRNA.

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CloneID	OBa08129
Clone ID Related Accession (Same CDS sequence)	XM_002693051.5 , XM_015466509.1 , XM_010812545.3 , XM_015466508.1 , XM_002693051.6 , XM_010812545.2
Accession Version	XM_015466509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321995.1
CDS	325..3693
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_015466509.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466509.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812545.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810847.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa08129
Clone ID Related Accession (Same CDS sequence)	XM_002693051.5 , XM_015466509.1 , XM_010812545.3 , XM_015466508.1 , XM_002693051.6 , XM_010812545.2
Accession Version	XM_010812545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810847.1
CDS	307..3675
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_010812545.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812545.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466508.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321994.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.

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CloneID	OBa08129
Clone ID Related Accession (Same CDS sequence)	XM_002693051.5 , XM_015466509.1 , XM_010812545.3 , XM_015466508.1 , XM_002693051.6 , XM_010812545.2
Accession Version	XM_015466508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321994.1
CDS	325..3693
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_015466508.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466508.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_002693051.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002693097.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.

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CloneID	OBa08129
Clone ID Related Accession (Same CDS sequence)	XM_002693051.5 , XM_015466509.1 , XM_010812545.3 , XM_015466508.1 , XM_002693051.6 , XM_010812545.2
Accession Version	XM_002693051.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002693051.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002693097.1
CDS	307..3675
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_002693051.6
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002693051.6

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812545.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810847.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.

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CloneID	OBa08129
Clone ID Related Accession (Same CDS sequence)	XM_002693051.5 , XM_015466509.1 , XM_010812545.3 , XM_015466508.1 , XM_002693051.6 , XM_010812545.2
Accession Version	XM_010812545.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810847.1
CDS	325..3693
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_010812545.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812545.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGTC CATTTCAGCC TTCCAGTGCT GCACCCACTT 
CTTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466510.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321996.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa08130
Clone ID Related Accession (Same CDS sequence)	XM_015466510.1 , XM_005216092.3 , XM_024975804.1 , XM_005216092.4
Accession Version	XM_015466510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321996.1
CDS	325..3630
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_015466510.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466510.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_005216092.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216149.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa08130
Clone ID Related Accession (Same CDS sequence)	XM_015466510.1 , XM_005216092.3 , XM_024975804.1 , XM_005216092.4
Accession Version	XM_005216092.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216149.1
CDS	325..3630
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_005216092.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216092.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_024975804.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831572.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa08130
Clone ID Related Accession (Same CDS sequence)	XM_015466510.1 , XM_005216092.3 , XM_024975804.1 , XM_005216092.4
Accession Version	XM_024975804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831572.1
CDS	307..3612
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_024975804.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975804.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_005216092.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216149.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa08130
Clone ID Related Accession (Same CDS sequence)	XM_015466510.1 , XM_005216092.3 , XM_024975804.1 , XM_005216092.4
Accession Version	XM_005216092.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216092.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216149.1
CDS	307..3612
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPVHFSLPVLHPLLG

Target ORF information:

RefSeq Version	XM_005216092.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216092.4

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGTCCAT TTCAGCCTTC CAGTGCTGCA CCCACTTCTT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812538.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810840.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.

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CloneID	OBa223227
Clone ID Related Accession (Same CDS sequence)	XM_010812538.3
Accession Version	XM_010812538.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTTGG AGAAGGCGCT GAGAGGAGAA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810840.2
CDS	314..6226
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRLEKALRGEADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812538.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812538.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTTGG AGAAGGCGCT GAGAGGAGAA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812541.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810843.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.

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CloneID	OBa223228
Clone ID Related Accession (Same CDS sequence)	XM_010812541.3
Accession Version	XM_010812541.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812541.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGAT 
GAGCCGACCT CCCCCAAGAA GCCCAAGAGC ATCCCGGAGC CTGAGCCTGG GGATGTAGCA 
GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG TGGACCGCAG TGAGAATCAG CCCCGGGGAG 
GATGTGGTTG GACAGGATGT GCTGGCCGTA CGCGTCCTGG TCACCAGCGA TGACAGCAGC 
TCCGATGCAG AATTGGACCG TGGTGACAGT GAGGCCTCCA GTGTGGAGCC CTTTGATGAA 
AGACCCCAGC AACCGGAACT CCAGCTCCCA CCCCTCCTGA AGCCCTTCAC CCGGCACAGC 
TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC TCTCCACATG ACCTCGGGGA GCCCGAAGCT 
GAGCCTGCTC TGCAGCGGGC CAATAGTTTC CAGTGTCCAA CTCCCAGCGA ATACCAGAGT 
GGGAGAAGAT TCCAGTCCAA TTTCATGCCG GTGAACAGCA AAATGACCGT GTCGCCTCCA 
AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT TCTCCACCCT CTTCTTCCTC GATTGGTGAC 
CTGAATGCCT TGGACAGTCC CTCCCTGCCT CAGGTGACAG TTTCCAACAC CGCCTCACAA 
ATCTCCAGAG GTCACTGCAG TCCATCCACC CCCATCTTTC TGAGGCGTGC CAAAGCCCAT 
GGACCACCCA AGGATCTCCC CCTGCGTCTG CCTCGTGGGC AGGTACTGGA GCGGACAGAA 
TACTGCCTGG TGAGGCCTGG TGGAAATGGC CTCCAGAGCT CCAGGCCACC CAGCCCTACA 
GAGATGGCTT CTGATGGGTG TCAGGAGGCA GTGGCCCCTC CTAGGGACAT CAGAAGCATC 
CACAGAGGCC CACACCCCGT TGAAGGGAAG GACAGGTGCT TGCCAGACCA CCCACTGTCT 
CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG AGTACCAGAC CCAGGAGGGT AGAGGATGGT 
GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG 
AAGACTTGGT TGTTGGATTG GAACGACTCC AACCTTGAGA ACTTGTACCT GGAAACCGGA 
GCACGACTTT CCCAAAAGAG CACTAGAAAT GGTTCAGGAG GCCATGTGCT GAAACCAGTC 
CACCCCTTGC TGCTTCCTCT GGCAGAGAAA GAGACCTTGC CAGCCCAGAG ACAGTCTCAG 
GAGAAGATGG GGGCCCCAGC TGAACGGGCT CCTGGGGAAC GAAGCATGGC CCCACCCAAG 
TCTCCACTGC GGCTTATAGC CAACGCCATC CGGAGATCCC TGGAGCCGCT CCTCCCCAAC 
TCTGACAGTG GGAGGAAGGC CTGGGCCAAG CCAGAGTCAA AGACTTTGCC CACCAGCCCA 
CCTCATGCTT ACACTTGCTC ATTCAGCTTT CGGAAAAGCA GCTCCAGTAA AGACAGCGAT 
CAGCCATCCC CCAAGAGAGA CGTGGCAAGC AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC 
CCGACTGCAA GGACTGCCCA GCCCTCAGCC CCGAGCCCTC CTGCCCCCGC CCTTTGCACT 
CACAGTTTGC CCAGCCGGTC ATCCAAGGTG TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC 
AAGATGGAAG ATGTCCCCAC ACTCCTGGAG AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT 
GCTTCCAGGG TTCCCAAGAA AAGGACCTCA CTCTTCTCCT CCTTCAGACT CAAAGACAAA 
TCTTTCGAGA GTTCCCTCCA AGAATCTGGA CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC 
AGCCCCAAGG GGAAGGTGCA GCCCATGGGC AATGCCCAGC CCCCAGAGAA GCAGGGGCAG 
CCAATCAGTA GCACCTGCCT GGGGCAGAGG GTCCATCCTG TTTCTCCAGA GAAAGATGCA 
AGTCCCACTC ACGTCCCCAT CAGAGCGCAG GGGACAGGGA CTGTCTCTTC CACCTCTTCC 
TCCACCACCT CCTCTGCAGA TGAGGAATTC CATCCCCAGC CTTCCTCGCG GTCAAAGGAG 
AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG GAAAAAGCAA CAAAGCAATT GGTTAAGCAA 
GAAGAATTGA AAAGACTTCA TAAAGCTCAG GCCATCCAGA GGCAGTTGGA GGAAGTGGAG 
GAGCAGCAGA GGGCTTTCGA GATCCAGGGC GTGAGGTTGG AGAAGGCGCT GAGAGGAGAA 
GCAGCAGATT CAGGCACACA GGATGAAGCA CAGCTTTTGC AGGAATGGCT TAAGCTGGTT 
CTGGAGAAGA ATAAATTAAT GCGATATGAG TCAGAGCTCC TGATCATGGC CCACGAACTA 
GAACTGGAAG ATCATCAAAG CAGACTAGAG CAGAAATTAA GAGAGAAAAT GCTCCAGGAA 
GAGAGCCAGA AAGATGAGAA TGATCTGAAT GAAGAGCAAG AAATACTCAC CGAGATGATG 
CAAGTGATTG AGCAAAGGGA CAAATTGGTG GATTCCTTAG AGGAACAACG CATCAGAGAG 
AAGGCCGAAG ACCAGCACTT TGAAAGCTTC ATATTCTCTA GGGGCTGTCA GCTGAGCAGG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810843.2
CDS	313..5658
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRLEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812541.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812541.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGAT 
GAGCCGACCT CCCCCAAGAA GCCCAAGAGC ATCCCGGAGC CTGAGCCTGG GGATGTAGCA 
GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG TGGACCGCAG TGAGAATCAG CCCCGGGGAG 
GATGTGGTTG GACAGGATGT GCTGGCCGTA CGCGTCCTGG TCACCAGCGA TGACAGCAGC 
TCCGATGCAG AATTGGACCG TGGTGACAGT GAGGCCTCCA GTGTGGAGCC CTTTGATGAA 
AGACCCCAGC AACCGGAACT CCAGCTCCCA CCCCTCCTGA AGCCCTTCAC CCGGCACAGC 
TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC TCTCCACATG ACCTCGGGGA GCCCGAAGCT 
GAGCCTGCTC TGCAGCGGGC CAATAGTTTC CAGTGTCCAA CTCCCAGCGA ATACCAGAGT 
GGGAGAAGAT TCCAGTCCAA TTTCATGCCG GTGAACAGCA AAATGACCGT GTCGCCTCCA 
AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT TCTCCACCCT CTTCTTCCTC GATTGGTGAC 
CTGAATGCCT TGGACAGTCC CTCCCTGCCT CAGGTGACAG TTTCCAACAC CGCCTCACAA 
ATCTCCAGAG GTCACTGCAG TCCATCCACC CCCATCTTTC TGAGGCGTGC CAAAGCCCAT 
GGACCACCCA AGGATCTCCC CCTGCGTCTG CCTCGTGGGC AGGTACTGGA GCGGACAGAA 
TACTGCCTGG TGAGGCCTGG TGGAAATGGC CTCCAGAGCT CCAGGCCACC CAGCCCTACA 
GAGATGGCTT CTGATGGGTG TCAGGAGGCA GTGGCCCCTC CTAGGGACAT CAGAAGCATC 
CACAGAGGCC CACACCCCGT TGAAGGGAAG GACAGGTGCT TGCCAGACCA CCCACTGTCT 
CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG AGTACCAGAC CCAGGAGGGT AGAGGATGGT 
GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG 
AAGACTTGGT TGTTGGATTG GAACGACTCC AACCTTGAGA ACTTGTACCT GGAAACCGGA 
GCACGACTTT CCCAAAAGAG CACTAGAAAT GGTTCAGGAG GCCATGTGCT GAAACCAGTC 
CACCCCTTGC TGCTTCCTCT GGCAGAGAAA GAGACCTTGC CAGCCCAGAG ACAGTCTCAG 
GAGAAGATGG GGGCCCCAGC TGAACGGGCT CCTGGGGAAC GAAGCATGGC CCCACCCAAG 
TCTCCACTGC GGCTTATAGC CAACGCCATC CGGAGATCCC TGGAGCCGCT CCTCCCCAAC 
TCTGACAGTG GGAGGAAGGC CTGGGCCAAG CCAGAGTCAA AGACTTTGCC CACCAGCCCA 
CCTCATGCTT ACACTTGCTC ATTCAGCTTT CGGAAAAGCA GCTCCAGTAA AGACAGCGAT 
CAGCCATCCC CCAAGAGAGA CGTGGCAAGC AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC 
CCGACTGCAA GGACTGCCCA GCCCTCAGCC CCGAGCCCTC CTGCCCCCGC CCTTTGCACT 
CACAGTTTGC CCAGCCGGTC ATCCAAGGTG TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC 
AAGATGGAAG ATGTCCCCAC ACTCCTGGAG AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT 
GCTTCCAGGG TTCCCAAGAA AAGGACCTCA CTCTTCTCCT CCTTCAGACT CAAAGACAAA 
TCTTTCGAGA GTTCCCTCCA AGAATCTGGA CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC 
AGCCCCAAGG GGAAGGTGCA GCCCATGGGC AATGCCCAGC CCCCAGAGAA GCAGGGGCAG 
CCAATCAGTA GCACCTGCCT GGGGCAGAGG GTCCATCCTG TTTCTCCAGA GAAAGATGCA 
AGTCCCACTC ACGTCCCCAT CAGAGCGCAG GGGACAGGGA CTGTCTCTTC CACCTCTTCC 
TCCACCACCT CCTCTGCAGA TGAGGAATTC CATCCCCAGC CTTCCTCGCG GTCAAAGGAG 
AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG GAAAAAGCAA CAAAGCAATT GGTTAAGCAA 
GAAGAATTGA AAAGACTTCA TAAAGCTCAG GCCATCCAGA GGCAGTTGGA GGAAGTGGAG 
GAGCAGCAGA GGGCTTTCGA GATCCAGGGC GTGAGGTTGG AGAAGGCGCT GAGAGGAGAA 
GCAGCAGATT CAGGCACACA GGATGAAGCA CAGCTTTTGC AGGAATGGCT TAAGCTGGTT 
CTGGAGAAGA ATAAATTAAT GCGATATGAG TCAGAGCTCC TGATCATGGC CCACGAACTA 
GAACTGGAAG ATCATCAAAG CAGACTAGAG CAGAAATTAA GAGAGAAAAT GCTCCAGGAA 
GAGAGCCAGA AAGATGAGAA TGATCTGAAT GAAGAGCAAG AAATACTCAC CGAGATGATG 
CAAGTGATTG AGCAAAGGGA CAAATTGGTG GATTCCTTAG AGGAACAACG CATCAGAGAG 
AAGGCCGAAG ACCAGCACTT TGAAAGCTTC ATATTCTCTA GGGGCTGTCA GCTGAGCAGG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812542.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810844.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.

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CloneID	OBa223229
Clone ID Related Accession (Same CDS sequence)	XM_010812542.3
Accession Version	XM_010812542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812542.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGCT GACAGTAGGG 
AAAGTATCCA GCGGAATAGG GGCTGCAGCT GAAGTTCTGG TCAATCTGTA CTTGAATGAT 
CACAGACCTA AGACTCAGGC CACCTCTCCA GACCTGGAGT CCGTTCGGAA GACGTTTCCC 
CTGAACGTGG GTGGCAGCGA CACCTGTTAC TTCTGCAAGA AGCGCGTGTA CGTCATGGAG 
CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC 
ACCACCTTGC ACCTGGCCAC CTACGCCTTC GACGTTGACG AAGGCAAGTT TTTCTGCAAG 
CCTCATTTCA TTCACTGTAA GACCAACAGC CAGCAACGGA AGAGACGGGC AGAGTTGAAG 
CAACAAAAAG AGGAGGAGGG GATGTGGAAA GAGCAAGAAG CCGCTAGGCG AGACCCTCCC 
TCGGAAAGTT CTTGCGCTGC CGCTGCCATC GCCACGCCGG AAGGCAGCCC CCCAGATGAG 
CCGACCTCCC CCAAGAAGCC CAAGAGCATC CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT 
GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG ACCGCAGTGA GAATCAGCCC CGGGGAGGAT 
GTGGTTGGAC AGGATGTGCT GGCCGTACGC GTCCTGGTCA CCAGCGATGA CAGCAGCTCC 
GATGCAGAAT TGGACCGTGG TGACAGTGAG GCCTCCAGTG TGGAGCCCTT TGATGAAAGA 
CCCCAGCAAC CGGAACTCCA GCTCCCACCC CTCCTGAAGC CCTTCACCCG GCACAGCTCC 
CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT CCACATGACC TCGGGGAGCC CGAAGCTGAG 
CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG TGTCCAACTC CCAGCGAATA CCAGAGTGGG 
AGAAGATTCC AGTCCAATTT CATGCCGGTG AACAGCAAAA TGACCGTGTC GCCTCCAAAG 
CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT CCACCCTCTT CTTCCTCGAT TGGTGACCTG 
AATGCCTTGG ACAGTCCCTC CCTGCCTCAG GTGACAGTTT CCAACACCGC CTCACAAATC 
TCCAGAGGTC ACTGCAGTCC ATCCACCCCC ATCTTTCTGA GGCGTGCCAA AGCCCATGGA 
CCACCCAAGG ATCTCCCCCT GCGTCTGCCT CGTGGGCAGG TACTGGAGCG GACAGAATAC 
TGCCTGGTGA GGCCTGGTGG AAATGGCCTC CAGAGCTCCA GGCCACCCAG CCCTACAGAG 
ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG GCCCCTCCTA GGGACATCAG AAGCATCCAC 
AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC AGGTGCTTGC CAGACCACCC ACTGTCTCCC 
TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT ACCAGACCCA GGAGGGTAGA GGATGGTGGG 
TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA AAGAAGTTGG TTCTCACTCC GGAGCAGAAG 
ACTTGGTTGT TGGATTGGAA CGACTCCAAC CTTGAGAACT TGTACCTGGA AACCGGAGCA 
CGACTTTCCC AAAAGAGCAC TAGAAATGGT TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC 
CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG ACCTTGCCAG CCCAGAGACA GTCTCAGGAG 
AAGATGGGGG CCCCAGCTGA ACGGGCTCCT GGGGAACGAA GCATGGCCCC ACCCAAGTCT 
CCACTGCGGC TTATAGCCAA CGCCATCCGG AGATCCCTGG AGCCGCTCCT CCCCAACTCT 
GACAGTGGGA GGAAGGCCTG GGCCAAGCCA GAGTCAAAGA CTTTGCCCAC CAGCCCACCT 
CATGCTTACA CTTGCTCATT CAGCTTTCGG AAAAGCAGCT CCAGTAAAGA CAGCGATCAG 
CCATCCCCCA AGAGAGACGT GGCAAGCAAG GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG 
ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG AGCCCTCCTG CCCCCGCCCT TTGCACTCAC 
AGTTTGCCCA GCCGGTCATC CAAGGTGTTT CCTGCACTTG TGCCCCCACC CTGTGGCAAG 
ATGGAAGATG TCCCCACACT CCTGGAGAAA GTGAGTTTGC AGGAGACAGT CCCAGATGCT 
TCCAGGGTTC CCAAGAAAAG GACCTCACTC TTCTCCTCCT TCAGACTCAA AGACAAATCT 
TTCGAGAGTT CCCTCCAAGA ATCTGGACCA AGAAAGGACT CCCAAGACCT CTTCAGCAGC 
CCCAAGGGGA AGGTGCAGCC CATGGGCAAT GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA 
ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC CATCCTGTTT CTCCAGAGAA AGATGCAAGT 
CCCACTCACG TCCCCATCAG AGCGCAGGGG ACAGGGACTG TCTCTTCCAC CTCTTCCTCC 
ACCACCTCCT CTGCAGATGA GGAATTCCAT CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG 
AAGGCACTTA GAAGAAGAAG GAAGCTGGAA AAAGCAACAA AGCAATTGGT TAAGCAAGAA 
GAATTGAAAA GACTTCATAA AGCTCAGGCC ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG 
CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG AGGTTGGAGA AGGCGCTGAG AGGAGAAGCA 
GCAGATTCAG GCACACAGGA TGAAGCACAG CTTTTGCAGG AATGGCTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCA GAGCTCCTGA TCATGGCCCA CGAACTAGAA 
CTGGAAGATC ATCAAAGCAG ACTAGAGCAG AAATTAAGAG AGAAAATGCT CCAGGAAGAG 
AGCCAGAAAG ATGAGAATGA TCTGAATGAA GAGCAAGAAA TACTCACCGA GATGATGCAA 
GTGATTGAGC AAAGGGACAA ATTGGTGGAT TCCTTAGAGG AACAACGCAT CAGAGAGAAG 
GCCGAAGACC AGCACTTTGA AAGCTTCATA TTCTCTAGGG GCTGTCAGCT GAGCAGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810844.2
CDS	313..5595
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRLEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812542.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812542.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGCT GACAGTAGGG 
AAAGTATCCA GCGGAATAGG GGCTGCAGCT GAAGTTCTGG TCAATCTGTA CTTGAATGAT 
CACAGACCTA AGACTCAGGC CACCTCTCCA GACCTGGAGT CCGTTCGGAA GACGTTTCCC 
CTGAACGTGG GTGGCAGCGA CACCTGTTAC TTCTGCAAGA AGCGCGTGTA CGTCATGGAG 
CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC 
ACCACCTTGC ACCTGGCCAC CTACGCCTTC GACGTTGACG AAGGCAAGTT TTTCTGCAAG 
CCTCATTTCA TTCACTGTAA GACCAACAGC CAGCAACGGA AGAGACGGGC AGAGTTGAAG 
CAACAAAAAG AGGAGGAGGG GATGTGGAAA GAGCAAGAAG CCGCTAGGCG AGACCCTCCC 
TCGGAAAGTT CTTGCGCTGC CGCTGCCATC GCCACGCCGG AAGGCAGCCC CCCAGATGAG 
CCGACCTCCC CCAAGAAGCC CAAGAGCATC CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT 
GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG ACCGCAGTGA GAATCAGCCC CGGGGAGGAT 
GTGGTTGGAC AGGATGTGCT GGCCGTACGC GTCCTGGTCA CCAGCGATGA CAGCAGCTCC 
GATGCAGAAT TGGACCGTGG TGACAGTGAG GCCTCCAGTG TGGAGCCCTT TGATGAAAGA 
CCCCAGCAAC CGGAACTCCA GCTCCCACCC CTCCTGAAGC CCTTCACCCG GCACAGCTCC 
CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT CCACATGACC TCGGGGAGCC CGAAGCTGAG 
CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG TGTCCAACTC CCAGCGAATA CCAGAGTGGG 
AGAAGATTCC AGTCCAATTT CATGCCGGTG AACAGCAAAA TGACCGTGTC GCCTCCAAAG 
CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT CCACCCTCTT CTTCCTCGAT TGGTGACCTG 
AATGCCTTGG ACAGTCCCTC CCTGCCTCAG GTGACAGTTT CCAACACCGC CTCACAAATC 
TCCAGAGGTC ACTGCAGTCC ATCCACCCCC ATCTTTCTGA GGCGTGCCAA AGCCCATGGA 
CCACCCAAGG ATCTCCCCCT GCGTCTGCCT CGTGGGCAGG TACTGGAGCG GACAGAATAC 
TGCCTGGTGA GGCCTGGTGG AAATGGCCTC CAGAGCTCCA GGCCACCCAG CCCTACAGAG 
ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG GCCCCTCCTA GGGACATCAG AAGCATCCAC 
AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC AGGTGCTTGC CAGACCACCC ACTGTCTCCC 
TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT ACCAGACCCA GGAGGGTAGA GGATGGTGGG 
TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA AAGAAGTTGG TTCTCACTCC GGAGCAGAAG 
ACTTGGTTGT TGGATTGGAA CGACTCCAAC CTTGAGAACT TGTACCTGGA AACCGGAGCA 
CGACTTTCCC AAAAGAGCAC TAGAAATGGT TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC 
CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG ACCTTGCCAG CCCAGAGACA GTCTCAGGAG 
AAGATGGGGG CCCCAGCTGA ACGGGCTCCT GGGGAACGAA GCATGGCCCC ACCCAAGTCT 
CCACTGCGGC TTATAGCCAA CGCCATCCGG AGATCCCTGG AGCCGCTCCT CCCCAACTCT 
GACAGTGGGA GGAAGGCCTG GGCCAAGCCA GAGTCAAAGA CTTTGCCCAC CAGCCCACCT 
CATGCTTACA CTTGCTCATT CAGCTTTCGG AAAAGCAGCT CCAGTAAAGA CAGCGATCAG 
CCATCCCCCA AGAGAGACGT GGCAAGCAAG GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG 
ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG AGCCCTCCTG CCCCCGCCCT TTGCACTCAC 
AGTTTGCCCA GCCGGTCATC CAAGGTGTTT CCTGCACTTG TGCCCCCACC CTGTGGCAAG 
ATGGAAGATG TCCCCACACT CCTGGAGAAA GTGAGTTTGC AGGAGACAGT CCCAGATGCT 
TCCAGGGTTC CCAAGAAAAG GACCTCACTC TTCTCCTCCT TCAGACTCAA AGACAAATCT 
TTCGAGAGTT CCCTCCAAGA ATCTGGACCA AGAAAGGACT CCCAAGACCT CTTCAGCAGC 
CCCAAGGGGA AGGTGCAGCC CATGGGCAAT GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA 
ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC CATCCTGTTT CTCCAGAGAA AGATGCAAGT 
CCCACTCACG TCCCCATCAG AGCGCAGGGG ACAGGGACTG TCTCTTCCAC CTCTTCCTCC 
ACCACCTCCT CTGCAGATGA GGAATTCCAT CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG 
AAGGCACTTA GAAGAAGAAG GAAGCTGGAA AAAGCAACAA AGCAATTGGT TAAGCAAGAA 
GAATTGAAAA GACTTCATAA AGCTCAGGCC ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG 
CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG AGGTTGGAGA AGGCGCTGAG AGGAGAAGCA 
GCAGATTCAG GCACACAGGA TGAAGCACAG CTTTTGCAGG AATGGCTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCA GAGCTCCTGA TCATGGCCCA CGAACTAGAA 
CTGGAAGATC ATCAAAGCAG ACTAGAGCAG AAATTAAGAG AGAAAATGCT CCAGGAAGAG 
AGCCAGAAAG ATGAGAATGA TCTGAATGAA GAGCAAGAAA TACTCACCGA GATGATGCAA 
GTGATTGAGC AAAGGGACAA ATTGGTGGAT TCCTTAGAGG AACAACGCAT CAGAGAGAAG 
GCCGAAGACC AGCACTTTGA AAGCTTCATA TTCTCTAGGG GCTGTCAGCT GAGCAGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812543.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810845.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.

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CloneID	OBa223230
Clone ID Related Accession (Same CDS sequence)	XM_010812543.3
Accession Version	XM_010812543.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG AAGCCCAAGA GCATCCCGGA GCCTGAGCCT 
GGGGATGTAG CAGGTGGAGC CGCCTCCCCC CTGCCCTCTG AGTGGACCGC AGTGAGAATC 
AGCCCCGGGG AGGATGTGGT TGGACAGGAT GTGCTGGCCG TACGCGTCCT GGTCACCAGC 
GATGACAGCA GCTCCGATGC AGAATTGGAC CGTGGTGACA GTGAGGCCTC CAGTGTGGAG 
CCCTTTGATG AAAGACCCCA GCAACCGGAA CTCCAGCTCC CACCCCTCCT GAAGCCCTTC 
ACCCGGCACA GCTCCCTGAG GGAGGCCCTG CCCAGGGCAG TCTCTCCACA TGACCTCGGG 
GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG GCCAATAGTT TCCAGTGTCC AACTCCCAGC 
GAATACCAGA GTGGGAGAAG ATTCCAGTCC AATTTCATGC CGGTGAACAG CAAAATGACC 
GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC CCGCCTTCTC CTTCTCCACC CTCTTCTTCC 
TCGATTGGTG ACCTGAATGC CTTGGACAGT CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC 
ACCGCCTCAC AAATCTCCAG AGGTCACTGC AGTCCATCCA CCCCCATCTT TCTGAGGCGT 
GCCAAAGCCC ATGGACCACC CAAGGATCTC CCCCTGCGTC TGCCTCGTGG GCAGGTACTG 
GAGCGGACAG AATACTGCCT GGTGAGGCCT GGTGGAAATG GCCTCCAGAG CTCCAGGCCA 
CCCAGCCCTA CAGAGATGGC TTCTGATGGG TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC 
ATCAGAAGCA TCCACAGAGG CCCACACCCC GTTGAAGGGA AGGACAGGTG CTTGCCAGAC 
CACCCACTGT CTCCCTGGGC TGGAGAGGAG CCTGGAGAAG GGAGTACCAG ACCCAGGAGG 
GTAGAGGATG GTGGGTTAGA GCTGGCAGAG GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC 
ACTCCGGAGC AGAAGACTTG GTTGTTGGAT TGGAACGACT CCAACCTTGA GAACTTGTAC 
CTGGAAACCG GAGCACGACT TTCCCAAAAG AGCACTAGAA ATGGTTCAGG AGGCCATGTG 
CTGAAACCAG TCCACCCCTT GCTGCTTCCT CTGGCAGAGA AAGAGACCTT GCCAGCCCAG 
AGACAGTCTC AGGAGAAGAT GGGGGCCCCA GCTGAACGGG CTCCTGGGGA ACGAAGCATG 
GCCCCACCCA AGTCTCCACT GCGGCTTATA GCCAACGCCA TCCGGAGATC CCTGGAGCCG 
CTCCTCCCCA ACTCTGACAG TGGGAGGAAG GCCTGGGCCA AGCCAGAGTC AAAGACTTTG 
CCCACCAGCC CACCTCATGC TTACACTTGC TCATTCAGCT TTCGGAAAAG CAGCTCCAGT 
AAAGACAGCG ATCAGCCATC CCCCAAGAGA GACGTGGCAA GCAAGGCCTC AGCCTTCTTC 
TCCCTGGGCT CCCCGACTGC AAGGACTGCC CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC 
GCCCTTTGCA CTCACAGTTT GCCCAGCCGG TCATCCAAGG TGTTTCCTGC ACTTGTGCCC 
CCACCCTGTG GCAAGATGGA AGATGTCCCC ACACTCCTGG AGAAAGTGAG TTTGCAGGAG 
ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG AAAAGGACCT CACTCTTCTC CTCCTTCAGA 
CTCAAAGACA AATCTTTCGA GAGTTCCCTC CAAGAATCTG GACCAAGAAA GGACTCCCAA 
GACCTCTTCA GCAGCCCCAA GGGGAAGGTG CAGCCCATGG GCAATGCCCA GCCCCCAGAG 
AAGCAGGGGC AGCCAATCAG TAGCACCTGC CTGGGGCAGA GGGTCCATCC TGTTTCTCCA 
GAGAAAGATG CAAGTCCCAC TCACGTCCCC ATCAGAGCGC AGGGGACAGG GACTGTCTCT 
TCCACCTCTT CCTCCACCAC CTCCTCTGCA GATGAGGAAT TCCATCCCCA GCCTTCCTCG 
CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA AGAAGGAAGC TGGAAAAAGC AACAAAGCAA 
TTGGTTAAGC AAGAAGAATT GAAAAGACTT CATAAAGCTC AGGCCATCCA GAGGCAGTTG 
GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC GAGATCCAGG GCGTGAGGTT GGAGAAGGCG 
CTGAGAGGAG AAGCAGCAGA TTCAGGCACA CAGGATGAAG CACAGCTTTT GCAGGAATGG 
CTTAAGCTGG TTCTGGAGAA GAATAAATTA ATGCGATATG AGTCAGAGCT CCTGATCATG 
GCCCACGAAC TAGAACTGGA AGATCATCAA AGCAGACTAG AGCAGAAATT AAGAGAGAAA 
ATGCTCCAGG AAGAGAGCCA GAAAGATGAG AATGATCTGA ATGAAGAGCA AGAAATACTC 
ACCGAGATGA TGCAAGTGAT TGAGCAAAGG GACAAATTGG TGGATTCCTT AGAGGAACAA 
CGCATCAGAG AGAAGGCCGA AGACCAGCAC TTTGAAAGCT TCATATTCTC TAGGGGCTGT 
CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810845.2
CDS	313..5550
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRLEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812543.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812543.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG AAGCCCAAGA GCATCCCGGA GCCTGAGCCT 
GGGGATGTAG CAGGTGGAGC CGCCTCCCCC CTGCCCTCTG AGTGGACCGC AGTGAGAATC 
AGCCCCGGGG AGGATGTGGT TGGACAGGAT GTGCTGGCCG TACGCGTCCT GGTCACCAGC 
GATGACAGCA GCTCCGATGC AGAATTGGAC CGTGGTGACA GTGAGGCCTC CAGTGTGGAG 
CCCTTTGATG AAAGACCCCA GCAACCGGAA CTCCAGCTCC CACCCCTCCT GAAGCCCTTC 
ACCCGGCACA GCTCCCTGAG GGAGGCCCTG CCCAGGGCAG TCTCTCCACA TGACCTCGGG 
GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG GCCAATAGTT TCCAGTGTCC AACTCCCAGC 
GAATACCAGA GTGGGAGAAG ATTCCAGTCC AATTTCATGC CGGTGAACAG CAAAATGACC 
GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC CCGCCTTCTC CTTCTCCACC CTCTTCTTCC 
TCGATTGGTG ACCTGAATGC CTTGGACAGT CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC 
ACCGCCTCAC AAATCTCCAG AGGTCACTGC AGTCCATCCA CCCCCATCTT TCTGAGGCGT 
GCCAAAGCCC ATGGACCACC CAAGGATCTC CCCCTGCGTC TGCCTCGTGG GCAGGTACTG 
GAGCGGACAG AATACTGCCT GGTGAGGCCT GGTGGAAATG GCCTCCAGAG CTCCAGGCCA 
CCCAGCCCTA CAGAGATGGC TTCTGATGGG TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC 
ATCAGAAGCA TCCACAGAGG CCCACACCCC GTTGAAGGGA AGGACAGGTG CTTGCCAGAC 
CACCCACTGT CTCCCTGGGC TGGAGAGGAG CCTGGAGAAG GGAGTACCAG ACCCAGGAGG 
GTAGAGGATG GTGGGTTAGA GCTGGCAGAG GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC 
ACTCCGGAGC AGAAGACTTG GTTGTTGGAT TGGAACGACT CCAACCTTGA GAACTTGTAC 
CTGGAAACCG GAGCACGACT TTCCCAAAAG AGCACTAGAA ATGGTTCAGG AGGCCATGTG 
CTGAAACCAG TCCACCCCTT GCTGCTTCCT CTGGCAGAGA AAGAGACCTT GCCAGCCCAG 
AGACAGTCTC AGGAGAAGAT GGGGGCCCCA GCTGAACGGG CTCCTGGGGA ACGAAGCATG 
GCCCCACCCA AGTCTCCACT GCGGCTTATA GCCAACGCCA TCCGGAGATC CCTGGAGCCG 
CTCCTCCCCA ACTCTGACAG TGGGAGGAAG GCCTGGGCCA AGCCAGAGTC AAAGACTTTG 
CCCACCAGCC CACCTCATGC TTACACTTGC TCATTCAGCT TTCGGAAAAG CAGCTCCAGT 
AAAGACAGCG ATCAGCCATC CCCCAAGAGA GACGTGGCAA GCAAGGCCTC AGCCTTCTTC 
TCCCTGGGCT CCCCGACTGC AAGGACTGCC CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC 
GCCCTTTGCA CTCACAGTTT GCCCAGCCGG TCATCCAAGG TGTTTCCTGC ACTTGTGCCC 
CCACCCTGTG GCAAGATGGA AGATGTCCCC ACACTCCTGG AGAAAGTGAG TTTGCAGGAG 
ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG AAAAGGACCT CACTCTTCTC CTCCTTCAGA 
CTCAAAGACA AATCTTTCGA GAGTTCCCTC CAAGAATCTG GACCAAGAAA GGACTCCCAA 
GACCTCTTCA GCAGCCCCAA GGGGAAGGTG CAGCCCATGG GCAATGCCCA GCCCCCAGAG 
AAGCAGGGGC AGCCAATCAG TAGCACCTGC CTGGGGCAGA GGGTCCATCC TGTTTCTCCA 
GAGAAAGATG CAAGTCCCAC TCACGTCCCC ATCAGAGCGC AGGGGACAGG GACTGTCTCT 
TCCACCTCTT CCTCCACCAC CTCCTCTGCA GATGAGGAAT TCCATCCCCA GCCTTCCTCG 
CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA AGAAGGAAGC TGGAAAAAGC AACAAAGCAA 
TTGGTTAAGC AAGAAGAATT GAAAAGACTT CATAAAGCTC AGGCCATCCA GAGGCAGTTG 
GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC GAGATCCAGG GCGTGAGGTT GGAGAAGGCG 
CTGAGAGGAG AAGCAGCAGA TTCAGGCACA CAGGATGAAG CACAGCTTTT GCAGGAATGG 
CTTAAGCTGG TTCTGGAGAA GAATAAATTA ATGCGATATG AGTCAGAGCT CCTGATCATG 
GCCCACGAAC TAGAACTGGA AGATCATCAA AGCAGACTAG AGCAGAAATT AAGAGAGAAA 
ATGCTCCAGG AAGAGAGCCA GAAAGATGAG AATGATCTGA ATGAAGAGCA AGAAATACTC 
ACCGAGATGA TGCAAGTGAT TGAGCAAAGG GACAAATTGG TGGATTCCTT AGAGGAACAA 
CGCATCAGAG AGAAGGCCGA AGACCAGCAC TTTGAAAGCT TCATATTCTC TAGGGGCTGT 
CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812544.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810846.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.

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CloneID	OBa223231
Clone ID Related Accession (Same CDS sequence)	XM_010812544.3
Accession Version	XM_010812544.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812544.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGATG AGCCGACCTC CCCCAAGAAG CCCAAGAGCA TCCCGGAGCC TGAGCCTGGG 
GATGTAGCAG GTGGAGCCGC CTCCCCCCTG CCCTCTGAGT GGACCGCAGT GAGAATCAGC 
CCCGGGGAGG ATGTGGTTGG ACAGGATGTG CTGGCCGTAC GCGTCCTGGT CACCAGCGAT 
GACAGCAGCT CCGATGCAGA ATTGGACCGT GGTGACAGTG AGGCCTCCAG TGTGGAGCCC 
TTTGATGAAA GACCCCAGCA ACCGGAACTC CAGCTCCCAC CCCTCCTGAA GCCCTTCACC 
CGGCACAGCT CCCTGAGGGA GGCCCTGCCC AGGGCAGTCT CTCCACATGA CCTCGGGGAG 
CCCGAAGCTG AGCCTGCTCT GCAGCGGGCC AATAGTTTCC AGTGTCCAAC TCCCAGCGAA 
TACCAGAGTG GGAGAAGATT CCAGTCCAAT TTCATGCCGG TGAACAGCAA AATGACCGTG 
TCGCCTCCAA AGCAAGCTTG TCCTCCCCCG CCTTCTCCTT CTCCACCCTC TTCTTCCTCG 
ATTGGTGACC TGAATGCCTT GGACAGTCCC TCCCTGCCTC AGGTGACAGT TTCCAACACC 
GCCTCACAAA TCTCCAGAGG TCACTGCAGT CCATCCACCC CCATCTTTCT GAGGCGTGCC 
AAAGCCCATG GACCACCCAA GGATCTCCCC CTGCGTCTGC CTCGTGGGCA GGTACTGGAG 
CGGACAGAAT ACTGCCTGGT GAGGCCTGGT GGAAATGGCC TCCAGAGCTC CAGGCCACCC 
AGCCCTACAG AGATGGCTTC TGATGGGTGT CAGGAGGCAG TGGCCCCTCC TAGGGACATC 
AGAAGCATCC ACAGAGGCCC ACACCCCGTT GAAGGGAAGG ACAGGTGCTT GCCAGACCAC 
CCACTGTCTC CCTGGGCTGG AGAGGAGCCT GGAGAAGGGA GTACCAGACC CAGGAGGGTA 
GAGGATGGTG GGTTAGAGCT GGCAGAGGAG AAGTCAGGCT TAAAGAAGTT GGTTCTCACT 
CCGGAGCAGA AGACTTGGTT GTTGGATTGG AACGACTCCA ACCTTGAGAA CTTGTACCTG 
GAAACCGGAG CACGACTTTC CCAAAAGAGC ACTAGAAATG GTTCAGGAGG CCATGTGCTG 
AAACCAGTCC ACCCCTTGCT GCTTCCTCTG GCAGAGAAAG AGACCTTGCC AGCCCAGAGA 
CAGTCTCAGG AGAAGATGGG GGCCCCAGCT GAACGGGCTC CTGGGGAACG AAGCATGGCC 
CCACCCAAGT CTCCACTGCG GCTTATAGCC AACGCCATCC GGAGATCCCT GGAGCCGCTC 
CTCCCCAACT CTGACAGTGG GAGGAAGGCC TGGGCCAAGC CAGAGTCAAA GACTTTGCCC 
ACCAGCCCAC CTCATGCTTA CACTTGCTCA TTCAGCTTTC GGAAAAGCAG CTCCAGTAAA 
GACAGCGATC AGCCATCCCC CAAGAGAGAC GTGGCAAGCA AGGCCTCAGC CTTCTTCTCC 
CTGGGCTCCC CGACTGCAAG GACTGCCCAG CCCTCAGCCC CGAGCCCTCC TGCCCCCGCC 
CTTTGCACTC ACAGTTTGCC CAGCCGGTCA TCCAAGGTGT TTCCTGCACT TGTGCCCCCA 
CCCTGTGGCA AGATGGAAGA TGTCCCCACA CTCCTGGAGA AAGTGAGTTT GCAGGAGACA 
GTCCCAGATG CTTCCAGGGT TCCCAAGAAA AGGACCTCAC TCTTCTCCTC CTTCAGACTC 
AAAGACAAAT CTTTCGAGAG TTCCCTCCAA GAATCTGGAC CAAGAAAGGA CTCCCAAGAC 
CTCTTCAGCA GCCCCAAGGG GAAGGTGCAG CCCATGGGCA ATGCCCAGCC CCCAGAGAAG 
CAGGGGCAGC CAATCAGTAG CACCTGCCTG GGGCAGAGGG TCCATCCTGT TTCTCCAGAG 
AAAGATGCAA GTCCCACTCA CGTCCCCATC AGAGCGCAGG GGACAGGGAC TGTCTCTTCC 
ACCTCTTCCT CCACCACCTC CTCTGCAGAT GAGGAATTCC ATCCCCAGCC TTCCTCGCGG 
TCAAAGGAGA GGAAGGCACT TAGAAGAAGA AGGAAGCTGG AAAAAGCAAC AAAGCAATTG 
GTTAAGCAAG AAGAATTGAA AAGACTTCAT AAAGCTCAGG CCATCCAGAG GCAGTTGGAG 
GAAGTGGAGG AGCAGCAGAG GGCTTTCGAG ATCCAGGGCG TGAGGTTGGA GAAGGCGCTG 
AGAGGAGAAG CAGCAGATTC AGGCACACAG GATGAAGCAC AGCTTTTGCA GGAATGGCTT 
AAGCTGGTTC TGGAGAAGAA TAAATTAATG CGATATGAGT CAGAGCTCCT GATCATGGCC 
CACGAACTAG AACTGGAAGA TCATCAAAGC AGACTAGAGC AGAAATTAAG AGAGAAAATG 
CTCCAGGAAG AGAGCCAGAA AGATGAGAAT GATCTGAATG AAGAGCAAGA AATACTCACC 
GAGATGATGC AAGTGATTGA GCAAAGGGAC AAATTGGTGG ATTCCTTAGA GGAACAACGC 
ATCAGAGAGA AGGCCGAAGA CCAGCACTTT GAAAGCTTCA TATTCTCTAG GGGCTGTCAG 
CTGAGCAGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810846.2
CDS	313..5487
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRLEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812544.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812544.3

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGATG AGCCGACCTC CCCCAAGAAG CCCAAGAGCA TCCCGGAGCC TGAGCCTGGG 
GATGTAGCAG GTGGAGCCGC CTCCCCCCTG CCCTCTGAGT GGACCGCAGT GAGAATCAGC 
CCCGGGGAGG ATGTGGTTGG ACAGGATGTG CTGGCCGTAC GCGTCCTGGT CACCAGCGAT 
GACAGCAGCT CCGATGCAGA ATTGGACCGT GGTGACAGTG AGGCCTCCAG TGTGGAGCCC 
TTTGATGAAA GACCCCAGCA ACCGGAACTC CAGCTCCCAC CCCTCCTGAA GCCCTTCACC 
CGGCACAGCT CCCTGAGGGA GGCCCTGCCC AGGGCAGTCT CTCCACATGA CCTCGGGGAG 
CCCGAAGCTG AGCCTGCTCT GCAGCGGGCC AATAGTTTCC AGTGTCCAAC TCCCAGCGAA 
TACCAGAGTG GGAGAAGATT CCAGTCCAAT TTCATGCCGG TGAACAGCAA AATGACCGTG 
TCGCCTCCAA AGCAAGCTTG TCCTCCCCCG CCTTCTCCTT CTCCACCCTC TTCTTCCTCG 
ATTGGTGACC TGAATGCCTT GGACAGTCCC TCCCTGCCTC AGGTGACAGT TTCCAACACC 
GCCTCACAAA TCTCCAGAGG TCACTGCAGT CCATCCACCC CCATCTTTCT GAGGCGTGCC 
AAAGCCCATG GACCACCCAA GGATCTCCCC CTGCGTCTGC CTCGTGGGCA GGTACTGGAG 
CGGACAGAAT ACTGCCTGGT GAGGCCTGGT GGAAATGGCC TCCAGAGCTC CAGGCCACCC 
AGCCCTACAG AGATGGCTTC TGATGGGTGT CAGGAGGCAG TGGCCCCTCC TAGGGACATC 
AGAAGCATCC ACAGAGGCCC ACACCCCGTT GAAGGGAAGG ACAGGTGCTT GCCAGACCAC 
CCACTGTCTC CCTGGGCTGG AGAGGAGCCT GGAGAAGGGA GTACCAGACC CAGGAGGGTA 
GAGGATGGTG GGTTAGAGCT GGCAGAGGAG AAGTCAGGCT TAAAGAAGTT GGTTCTCACT 
CCGGAGCAGA AGACTTGGTT GTTGGATTGG AACGACTCCA ACCTTGAGAA CTTGTACCTG 
GAAACCGGAG CACGACTTTC CCAAAAGAGC ACTAGAAATG GTTCAGGAGG CCATGTGCTG 
AAACCAGTCC ACCCCTTGCT GCTTCCTCTG GCAGAGAAAG AGACCTTGCC AGCCCAGAGA 
CAGTCTCAGG AGAAGATGGG GGCCCCAGCT GAACGGGCTC CTGGGGAACG AAGCATGGCC 
CCACCCAAGT CTCCACTGCG GCTTATAGCC AACGCCATCC GGAGATCCCT GGAGCCGCTC 
CTCCCCAACT CTGACAGTGG GAGGAAGGCC TGGGCCAAGC CAGAGTCAAA GACTTTGCCC 
ACCAGCCCAC CTCATGCTTA CACTTGCTCA TTCAGCTTTC GGAAAAGCAG CTCCAGTAAA 
GACAGCGATC AGCCATCCCC CAAGAGAGAC GTGGCAAGCA AGGCCTCAGC CTTCTTCTCC 
CTGGGCTCCC CGACTGCAAG GACTGCCCAG CCCTCAGCCC CGAGCCCTCC TGCCCCCGCC 
CTTTGCACTC ACAGTTTGCC CAGCCGGTCA TCCAAGGTGT TTCCTGCACT TGTGCCCCCA 
CCCTGTGGCA AGATGGAAGA TGTCCCCACA CTCCTGGAGA AAGTGAGTTT GCAGGAGACA 
GTCCCAGATG CTTCCAGGGT TCCCAAGAAA AGGACCTCAC TCTTCTCCTC CTTCAGACTC 
AAAGACAAAT CTTTCGAGAG TTCCCTCCAA GAATCTGGAC CAAGAAAGGA CTCCCAAGAC 
CTCTTCAGCA GCCCCAAGGG GAAGGTGCAG CCCATGGGCA ATGCCCAGCC CCCAGAGAAG 
CAGGGGCAGC CAATCAGTAG CACCTGCCTG GGGCAGAGGG TCCATCCTGT TTCTCCAGAG 
AAAGATGCAA GTCCCACTCA CGTCCCCATC AGAGCGCAGG GGACAGGGAC TGTCTCTTCC 
ACCTCTTCCT CCACCACCTC CTCTGCAGAT GAGGAATTCC ATCCCCAGCC TTCCTCGCGG 
TCAAAGGAGA GGAAGGCACT TAGAAGAAGA AGGAAGCTGG AAAAAGCAAC AAAGCAATTG 
GTTAAGCAAG AAGAATTGAA AAGACTTCAT AAAGCTCAGG CCATCCAGAG GCAGTTGGAG 
GAAGTGGAGG AGCAGCAGAG GGCTTTCGAG ATCCAGGGCG TGAGGTTGGA GAAGGCGCTG 
AGAGGAGAAG CAGCAGATTC AGGCACACAG GATGAAGCAC AGCTTTTGCA GGAATGGCTT 
AAGCTGGTTC TGGAGAAGAA TAAATTAATG CGATATGAGT CAGAGCTCCT GATCATGGCC 
CACGAACTAG AACTGGAAGA TCATCAAAGC AGACTAGAGC AGAAATTAAG AGAGAAAATG 
CTCCAGGAAG AGAGCCAGAA AGATGAGAAT GATCTGAATG AAGAGCAAGA AATACTCACC 
GAGATGATGC AAGTGATTGA GCAAAGGGAC AAATTGGTGG ATTCCTTAGA GGAACAACGC 
ATCAGAGAGA AGGCCGAAGA CCAGCACTTT GAAAGCTTCA TATTCTCTAG GGGCTGTCAG 
CTGAGCAGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466500.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321986.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.

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CloneID	OBa92667
Clone ID Related Accession (Same CDS sequence)	XM_015466500.1 , XM_015474698.1
Accession Version	XM_015466500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGATGAG CCGACCTCCC CCAAGAAGCC CAAGAGCATC 
CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG 
ACCGCAGTGA GAATCAGCCC CGGGGAGGAT GTGGTTGGAC AGGATGTGCT GGCCGTACGC 
GTCCTGGTCA CCAGCGATGA CAGCAGCTCC GATGCAGAAT TGGACCGTGG TGACAGTGAG 
GCCTCCAGTG TGGAGCCCTT TGATGAAAGA CCCCAGCAAC CGGAACTCCA GCTCCCACCC 
CTCCTGAAGC CCTTCACCCG GCACAGCTCC CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT 
CCACATGACC TCGGGGAGCC CGAAGCTGAG CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG 
TGTCCAACTC CCAGCGAATA CCAGAGTGGG AGAAGATTCC AGTCCAATTT CATGCCGGTG 
AACAGCAAAA TGACCGTGTC GCCTCCAAAG CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT 
CCACCCTCTT CTTCCTCGAT TGGTGACCTG AATGCCTTGG ACAGTCCCTC CCTGCCTCAG 
GTGACAGTTT CCAACACCGC CTCACAAATC TCCAGAGGTC ACTGCAGTCC ATCCACCCCC 
ATCTTTCTGA GGCGTGCCAA AGCCCATGGA CCACCCAAGG ATCTCCCCCT GCGTCTGCCT 
CGTGGGCAGG TACTGGAGCG GACAGAATAC TGCCTGGTGA GGCCTGGTGG AAATGGCCTC 
CAGAGCTCCA GGCCACCCAG CCCTACAGAG ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG 
GCCCCTCCTA GGGACATCAG AAGCATCCAC AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC 
AGGTGCTTGC CAGACCACCC ACTGTCTCCC TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT 
ACCAGACCCA GGAGGGTAGA GGATGGTGGG TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA 
AAGAAGTTGG TTCTCACTCC GGAGCAGAAG ACTTGGTTGT TGGATTGGAA CGACTCCAAC 
CTTGAGAACT TGTACCTGGA AACCGGAGCA CGACTTTCCC AAAAGAGCAC TAGAAATGGT 
TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG 
ACCTTGCCAG CCCAGAGACA GTCTCAGGAG AAGATGGGGG CCCCAGCTGA ACGGGCTCCT 
GGGGAACGAA GCATGGCCCC ACCCAAGTCT CCACTGCGGC TTATAGCCAA CGCCATCCGG 
AGATCCCTGG AGCCGCTCCT CCCCAACTCT GACAGTGGGA GGAAGGCCTG GGCCAAGCCA 
GAGTCAAAGA CTTTGCCCAC CAGCCCACCT CATGCTTACA CTTGCTCATT CAGCTTTCGG 
AAAAGCAGCT CCAGTAAAGA CAGCGATCAG CCATCCCCCA AGAGAGACGT GGCAAGCAAG 
GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG 
AGCCCTCCTG CCCCCGCCCT TTGCACTCAC AGTTTGCCCA GCCGGTCATC CAAGGTGTTT 
CCTGCACTTG TGCCCCCACC CTGTGGCAAG ATGGAAGATG TCCCCACACT CCTGGAGAAA 
GTGAGTTTGC AGGAGACAGT CCCAGATGCT TCCAGGGTTC CCAAGAAAAG GACCTCACTC 
TTCTCCTCCT TCAGACTCAA AGACAAATCT TTCGAGAGTT CCCTCCAAGA ATCTGGACCA 
AGAAAGGACT CCCAAGACCT CTTCAGCAGC CCCAAGGGGA AGGTGCAGCC CATGGGCAAT 
GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC 
CATCCTGTTT CTCCAGAGAA AGATGCAAGT CCCACTCACG TCCCCATCAG AGCGCAGGGG 
ACAGGGACTG TCTCTTCCAC CTCTTCCTCC ACCACCTCCT CTGCAGATGA GGAATTCCAT 
CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG AAGGCACTTA GAAGAAGAAG GAAGCTGGAA 
AAAGCAACAA AGCAATTGGT TAAGCAAGAA GAATTGAAAA GACTTCATAA AGCTCAGGCC 
ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG 
AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321986.1
CDS	330..6182
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466500.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466500.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGATGAG CCGACCTCCC CCAAGAAGCC CAAGAGCATC 
CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG 
ACCGCAGTGA GAATCAGCCC CGGGGAGGAT GTGGTTGGAC AGGATGTGCT GGCCGTACGC 
GTCCTGGTCA CCAGCGATGA CAGCAGCTCC GATGCAGAAT TGGACCGTGG TGACAGTGAG 
GCCTCCAGTG TGGAGCCCTT TGATGAAAGA CCCCAGCAAC CGGAACTCCA GCTCCCACCC 
CTCCTGAAGC CCTTCACCCG GCACAGCTCC CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT 
CCACATGACC TCGGGGAGCC CGAAGCTGAG CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG 
TGTCCAACTC CCAGCGAATA CCAGAGTGGG AGAAGATTCC AGTCCAATTT CATGCCGGTG 
AACAGCAAAA TGACCGTGTC GCCTCCAAAG CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT 
CCACCCTCTT CTTCCTCGAT TGGTGACCTG AATGCCTTGG ACAGTCCCTC CCTGCCTCAG 
GTGACAGTTT CCAACACCGC CTCACAAATC TCCAGAGGTC ACTGCAGTCC ATCCACCCCC 
ATCTTTCTGA GGCGTGCCAA AGCCCATGGA CCACCCAAGG ATCTCCCCCT GCGTCTGCCT 
CGTGGGCAGG TACTGGAGCG GACAGAATAC TGCCTGGTGA GGCCTGGTGG AAATGGCCTC 
CAGAGCTCCA GGCCACCCAG CCCTACAGAG ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG 
GCCCCTCCTA GGGACATCAG AAGCATCCAC AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC 
AGGTGCTTGC CAGACCACCC ACTGTCTCCC TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT 
ACCAGACCCA GGAGGGTAGA GGATGGTGGG TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA 
AAGAAGTTGG TTCTCACTCC GGAGCAGAAG ACTTGGTTGT TGGATTGGAA CGACTCCAAC 
CTTGAGAACT TGTACCTGGA AACCGGAGCA CGACTTTCCC AAAAGAGCAC TAGAAATGGT 
TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG 
ACCTTGCCAG CCCAGAGACA GTCTCAGGAG AAGATGGGGG CCCCAGCTGA ACGGGCTCCT 
GGGGAACGAA GCATGGCCCC ACCCAAGTCT CCACTGCGGC TTATAGCCAA CGCCATCCGG 
AGATCCCTGG AGCCGCTCCT CCCCAACTCT GACAGTGGGA GGAAGGCCTG GGCCAAGCCA 
GAGTCAAAGA CTTTGCCCAC CAGCCCACCT CATGCTTACA CTTGCTCATT CAGCTTTCGG 
AAAAGCAGCT CCAGTAAAGA CAGCGATCAG CCATCCCCCA AGAGAGACGT GGCAAGCAAG 
GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG 
AGCCCTCCTG CCCCCGCCCT TTGCACTCAC AGTTTGCCCA GCCGGTCATC CAAGGTGTTT 
CCTGCACTTG TGCCCCCACC CTGTGGCAAG ATGGAAGATG TCCCCACACT CCTGGAGAAA 
GTGAGTTTGC AGGAGACAGT CCCAGATGCT TCCAGGGTTC CCAAGAAAAG GACCTCACTC 
TTCTCCTCCT TCAGACTCAA AGACAAATCT TTCGAGAGTT CCCTCCAAGA ATCTGGACCA 
AGAAAGGACT CCCAAGACCT CTTCAGCAGC CCCAAGGGGA AGGTGCAGCC CATGGGCAAT 
GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC 
CATCCTGTTT CTCCAGAGAA AGATGCAAGT CCCACTCACG TCCCCATCAG AGCGCAGGGG 
ACAGGGACTG TCTCTTCCAC CTCTTCCTCC ACCACCTCCT CTGCAGATGA GGAATTCCAT 
CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG AAGGCACTTA GAAGAAGAAG GAAGCTGGAA 
AAAGCAACAA AGCAATTGGT TAAGCAAGAA GAATTGAAAA GACTTCATAA AGCTCAGGCC 
ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG 
AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015474698.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330184.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.

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CloneID	OBa92667
Clone ID Related Accession (Same CDS sequence)	XM_015466500.1 , XM_015474698.1
Accession Version	XM_015474698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGATGAG CCGACCTCCC CCAAGAAGCC CAAGAGCATC 
CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG 
ACCGCAGTGA GAATCAGCCC CGGGGAGGAT GTGGTTGGAC AGGATGTGCT GGCCGTACGC 
GTCCTGGTCA CCAGCGATGA CAGCAGCTCC GATGCAGAAT TGGACCGTGG TGACAGTGAG 
GCCTCCAGTG TGGAGCCCTT TGATGAAAGA CCCCAGCAAC CGGAACTCCA GCTCCCACCC 
CTCCTGAAGC CCTTCACCCG GCACAGCTCC CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT 
CCACATGACC TCGGGGAGCC CGAAGCTGAG CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG 
TGTCCAACTC CCAGCGAATA CCAGAGTGGG AGAAGATTCC AGTCCAATTT CATGCCGGTG 
AACAGCAAAA TGACCGTGTC GCCTCCAAAG CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT 
CCACCCTCTT CTTCCTCGAT TGGTGACCTG AATGCCTTGG ACAGTCCCTC CCTGCCTCAG 
GTGACAGTTT CCAACACCGC CTCACAAATC TCCAGAGGTC ACTGCAGTCC ATCCACCCCC 
ATCTTTCTGA GGCGTGCCAA AGCCCATGGA CCACCCAAGG ATCTCCCCCT GCGTCTGCCT 
CGTGGGCAGG TACTGGAGCG GACAGAATAC TGCCTGGTGA GGCCTGGTGG AAATGGCCTC 
CAGAGCTCCA GGCCACCCAG CCCTACAGAG ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG 
GCCCCTCCTA GGGACATCAG AAGCATCCAC AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC 
AGGTGCTTGC CAGACCACCC ACTGTCTCCC TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT 
ACCAGACCCA GGAGGGTAGA GGATGGTGGG TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA 
AAGAAGTTGG TTCTCACTCC GGAGCAGAAG ACTTGGTTGT TGGATTGGAA CGACTCCAAC 
CTTGAGAACT TGTACCTGGA AACCGGAGCA CGACTTTCCC AAAAGAGCAC TAGAAATGGT 
TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG 
ACCTTGCCAG CCCAGAGACA GTCTCAGGAG AAGATGGGGG CCCCAGCTGA ACGGGCTCCT 
GGGGAACGAA GCATGGCCCC ACCCAAGTCT CCACTGCGGC TTATAGCCAA CGCCATCCGG 
AGATCCCTGG AGCCGCTCCT CCCCAACTCT GACAGTGGGA GGAAGGCCTG GGCCAAGCCA 
GAGTCAAAGA CTTTGCCCAC CAGCCCACCT CATGCTTACA CTTGCTCATT CAGCTTTCGG 
AAAAGCAGCT CCAGTAAAGA CAGCGATCAG CCATCCCCCA AGAGAGACGT GGCAAGCAAG 
GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG 
AGCCCTCCTG CCCCCGCCCT TTGCACTCAC AGTTTGCCCA GCCGGTCATC CAAGGTGTTT 
CCTGCACTTG TGCCCCCACC CTGTGGCAAG ATGGAAGATG TCCCCACACT CCTGGAGAAA 
GTGAGTTTGC AGGAGACAGT CCCAGATGCT TCCAGGGTTC CCAAGAAAAG GACCTCACTC 
TTCTCCTCCT TCAGACTCAA AGACAAATCT TTCGAGAGTT CCCTCCAAGA ATCTGGACCA 
AGAAAGGACT CCCAAGACCT CTTCAGCAGC CCCAAGGGGA AGGTGCAGCC CATGGGCAAT 
GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC 
CATCCTGTTT CTCCAGAGAA AGATGCAAGT CCCACTCACG TCCCCATCAG AGCGCAGGGG 
ACAGGGACTG TCTCTTCCAC CTCTTCCTCC ACCACCTCCT CTGCAGATGA GGAATTCCAT 
CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG AAGGCACTTA GAAGAAGAAG GAAGCTGGAA 
AAAGCAACAA AGCAATTGGT TAAGCAAGAA GAATTGAAAA GACTTCATAA AGCTCAGGCC 
ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG 
AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330184.1
CDS	330..6182
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015474698.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474698.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGCT GACAGTAGGG AAAGTATCCA GCGGAATAGG GGCTGCAGCT 
GAAGTTCTGG TCAATCTGTA CTTGAATGAT CACAGACCTA AGACTCAGGC CACCTCTCCA 
GACCTGGAGT CCGTTCGGAA GACGTTTCCC CTGAACGTGG GTGGCAGCGA CACCTGTTAC 
TTCTGCAAGA AGCGCGTGTA CGTCATGGAG CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC 
CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC ACCACCTTGC ACCTGGCCAC CTACGCCTTC 
GACGTTGACG AAGGCAAGTT TTTCTGCAAG CCTCATTTCA TTCACTGTAA GACCAACAGC 
CAGCAACGGA AGAGACGGGC AGAGTTGAAG CAACAAAAAG AGGAGGAGGG GATGTGGAAA 
GAGCAAGAAG CCGCTAGGCG AGACCCTCCC TCGGAAAGTT CTTGCGCTGC CGCTGCCATC 
GCCACGCCGG AAGGCAGCCC CCCAGATGAG CCGACCTCCC CCAAGAAGCC CAAGAGCATC 
CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG 
ACCGCAGTGA GAATCAGCCC CGGGGAGGAT GTGGTTGGAC AGGATGTGCT GGCCGTACGC 
GTCCTGGTCA CCAGCGATGA CAGCAGCTCC GATGCAGAAT TGGACCGTGG TGACAGTGAG 
GCCTCCAGTG TGGAGCCCTT TGATGAAAGA CCCCAGCAAC CGGAACTCCA GCTCCCACCC 
CTCCTGAAGC CCTTCACCCG GCACAGCTCC CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT 
CCACATGACC TCGGGGAGCC CGAAGCTGAG CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG 
TGTCCAACTC CCAGCGAATA CCAGAGTGGG AGAAGATTCC AGTCCAATTT CATGCCGGTG 
AACAGCAAAA TGACCGTGTC GCCTCCAAAG CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT 
CCACCCTCTT CTTCCTCGAT TGGTGACCTG AATGCCTTGG ACAGTCCCTC CCTGCCTCAG 
GTGACAGTTT CCAACACCGC CTCACAAATC TCCAGAGGTC ACTGCAGTCC ATCCACCCCC 
ATCTTTCTGA GGCGTGCCAA AGCCCATGGA CCACCCAAGG ATCTCCCCCT GCGTCTGCCT 
CGTGGGCAGG TACTGGAGCG GACAGAATAC TGCCTGGTGA GGCCTGGTGG AAATGGCCTC 
CAGAGCTCCA GGCCACCCAG CCCTACAGAG ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG 
GCCCCTCCTA GGGACATCAG AAGCATCCAC AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC 
AGGTGCTTGC CAGACCACCC ACTGTCTCCC TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT 
ACCAGACCCA GGAGGGTAGA GGATGGTGGG TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA 
AAGAAGTTGG TTCTCACTCC GGAGCAGAAG ACTTGGTTGT TGGATTGGAA CGACTCCAAC 
CTTGAGAACT TGTACCTGGA AACCGGAGCA CGACTTTCCC AAAAGAGCAC TAGAAATGGT 
TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG 
ACCTTGCCAG CCCAGAGACA GTCTCAGGAG AAGATGGGGG CCCCAGCTGA ACGGGCTCCT 
GGGGAACGAA GCATGGCCCC ACCCAAGTCT CCACTGCGGC TTATAGCCAA CGCCATCCGG 
AGATCCCTGG AGCCGCTCCT CCCCAACTCT GACAGTGGGA GGAAGGCCTG GGCCAAGCCA 
GAGTCAAAGA CTTTGCCCAC CAGCCCACCT CATGCTTACA CTTGCTCATT CAGCTTTCGG 
AAAAGCAGCT CCAGTAAAGA CAGCGATCAG CCATCCCCCA AGAGAGACGT GGCAAGCAAG 
GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG 
AGCCCTCCTG CCCCCGCCCT TTGCACTCAC AGTTTGCCCA GCCGGTCATC CAAGGTGTTT 
CCTGCACTTG TGCCCCCACC CTGTGGCAAG ATGGAAGATG TCCCCACACT CCTGGAGAAA 
GTGAGTTTGC AGGAGACAGT CCCAGATGCT TCCAGGGTTC CCAAGAAAAG GACCTCACTC 
TTCTCCTCCT TCAGACTCAA AGACAAATCT TTCGAGAGTT CCCTCCAAGA ATCTGGACCA 
AGAAAGGACT CCCAAGACCT CTTCAGCAGC CCCAAGGGGA AGGTGCAGCC CATGGGCAAT 
GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC 
CATCCTGTTT CTCCAGAGAA AGATGCAAGT CCCACTCACG TCCCCATCAG AGCGCAGGGG 
ACAGGGACTG TCTCTTCCAC CTCTTCCTCC ACCACCTCCT CTGCAGATGA GGAATTCCAT 
CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG AAGGCACTTA GAAGAAGAAG GAAGCTGGAA 
AAAGCAACAA AGCAATTGGT TAAGCAAGAA GAATTGAAAA GACTTCATAA AGCTCAGGCC 
ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG 
AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466502.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321988.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.

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CloneID	OBa92668
Clone ID Related Accession (Same CDS sequence)	XM_015466502.1 , XM_015474699.1
Accession Version	XM_015466502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GGAGTCCGTT CGGAAGACGT TTCCCCTGAA CGTGGGTGGC 
AGCGACACCT GTTACTTCTG CAAGAAGCGC GTGTACGTCA TGGAGCGGCT GAGCGCGGAG 
GGCCGCTTCT TCCACCGGGA GTGTTTCCGC TGCAGCGTCT GCGCCACCAC CTTGCACCTG 
GCCACCTACG CCTTCGACGT TGACGAAGGC AAGTTTTTCT GCAAGCCTCA TTTCATTCAC 
TGTAAGACCA ACAGCCAGCA ACGGAAGAGA CGGGCAGAGT TGAAGCAACA AAAAGAGGAG 
GAGGGGATGT GGAAAGAGCA AGAAGCCGCT AGGCGAGACC CTCCCTCGGA AAGTTCTTGC 
GCTGCCGCTG CCATCGCCAC GCCGGAAGGC AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG 
AAGCCCAAGA GCATCCCGGA GCCTGAGCCT GGGGATGTAG CAGGTGGAGC CGCCTCCCCC 
CTGCCCTCTG AGTGGACCGC AGTGAGAATC AGCCCCGGGG AGGATGTGGT TGGACAGGAT 
GTGCTGGCCG TACGCGTCCT GGTCACCAGC GATGACAGCA GCTCCGATGC AGAATTGGAC 
CGTGGTGACA GTGAGGCCTC CAGTGTGGAG CCCTTTGATG AAAGACCCCA GCAACCGGAA 
CTCCAGCTCC CACCCCTCCT GAAGCCCTTC ACCCGGCACA GCTCCCTGAG GGAGGCCCTG 
CCCAGGGCAG TCTCTCCACA TGACCTCGGG GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG 
GCCAATAGTT TCCAGTGTCC AACTCCCAGC GAATACCAGA GTGGGAGAAG ATTCCAGTCC 
AATTTCATGC CGGTGAACAG CAAAATGACC GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC 
CCGCCTTCTC CTTCTCCACC CTCTTCTTCC TCGATTGGTG ACCTGAATGC CTTGGACAGT 
CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC ACCGCCTCAC AAATCTCCAG AGGTCACTGC 
AGTCCATCCA CCCCCATCTT TCTGAGGCGT GCCAAAGCCC ATGGACCACC CAAGGATCTC 
CCCCTGCGTC TGCCTCGTGG GCAGGTACTG GAGCGGACAG AATACTGCCT GGTGAGGCCT 
GGTGGAAATG GCCTCCAGAG CTCCAGGCCA CCCAGCCCTA CAGAGATGGC TTCTGATGGG 
TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC ATCAGAAGCA TCCACAGAGG CCCACACCCC 
GTTGAAGGGA AGGACAGGTG CTTGCCAGAC CACCCACTGT CTCCCTGGGC TGGAGAGGAG 
CCTGGAGAAG GGAGTACCAG ACCCAGGAGG GTAGAGGATG GTGGGTTAGA GCTGGCAGAG 
GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC ACTCCGGAGC AGAAGACTTG GTTGTTGGAT 
TGGAACGACT CCAACCTTGA GAACTTGTAC CTGGAAACCG GAGCACGACT TTCCCAAAAG 
AGCACTAGAA ATGGTTCAGG AGGCCATGTG CTGAAACCAG TCCACCCCTT GCTGCTTCCT 
CTGGCAGAGA AAGAGACCTT GCCAGCCCAG AGACAGTCTC AGGAGAAGAT GGGGGCCCCA 
GCTGAACGGG CTCCTGGGGA ACGAAGCATG GCCCCACCCA AGTCTCCACT GCGGCTTATA 
GCCAACGCCA TCCGGAGATC CCTGGAGCCG CTCCTCCCCA ACTCTGACAG TGGGAGGAAG 
GCCTGGGCCA AGCCAGAGTC AAAGACTTTG CCCACCAGCC CACCTCATGC TTACACTTGC 
TCATTCAGCT TTCGGAAAAG CAGCTCCAGT AAAGACAGCG ATCAGCCATC CCCCAAGAGA 
GACGTGGCAA GCAAGGCCTC AGCCTTCTTC TCCCTGGGCT CCCCGACTGC AAGGACTGCC 
CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC GCCCTTTGCA CTCACAGTTT GCCCAGCCGG 
TCATCCAAGG TGTTTCCTGC ACTTGTGCCC CCACCCTGTG GCAAGATGGA AGATGTCCCC 
ACACTCCTGG AGAAAGTGAG TTTGCAGGAG ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG 
AAAAGGACCT CACTCTTCTC CTCCTTCAGA CTCAAAGACA AATCTTTCGA GAGTTCCCTC 
CAAGAATCTG GACCAAGAAA GGACTCCCAA GACCTCTTCA GCAGCCCCAA GGGGAAGGTG 
CAGCCCATGG GCAATGCCCA GCCCCCAGAG AAGCAGGGGC AGCCAATCAG TAGCACCTGC 
CTGGGGCAGA GGGTCCATCC TGTTTCTCCA GAGAAAGATG CAAGTCCCAC TCACGTCCCC 
ATCAGAGCGC AGGGGACAGG GACTGTCTCT TCCACCTCTT CCTCCACCAC CTCCTCTGCA 
GATGAGGAAT TCCATCCCCA GCCTTCCTCG CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA 
AGAAGGAAGC TGGAAAAAGC AACAAAGCAA TTGGTTAAGC AAGAAGAATT GAAAAGACTT 
CATAAAGCTC AGGCCATCCA GAGGCAGTTG GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC 
GAGATCCAGG GCGTGAGGTG GGAGAAGGCG CTGAGAGGAG AAGCAGCAGA TTCAGGCACA 
CAGGATGAAG CACAGCTTTT GCAGGAATGG CTTAAGCTGG TTCTGGAGAA GAATAAATTA 
ATGCGATATG AGTCAGAGCT CCTGATCATG GCCCACGAAC TAGAACTGGA AGATCATCAA 
AGCAGACTAG AGCAGAAATT AAGAGAGAAA ATGCTCCAGG AAGAGAGCCA GAAAGATGAG 
AATGATCTGA ATGAAGAGCA AGAAATACTC ACCGAGATGA TGCAAGTGAT TGAGCAAAGG 
GACAAATTGG TGGATTCCTT AGAGGAACAA CGCATCAGAG AGAAGGCCGA AGACCAGCAC 
TTTGAAAGCT TCATATTCTC TAGGGGCTGT CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321988.1
CDS	330..6137
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466502.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466502.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GGAGTCCGTT CGGAAGACGT TTCCCCTGAA CGTGGGTGGC 
AGCGACACCT GTTACTTCTG CAAGAAGCGC GTGTACGTCA TGGAGCGGCT GAGCGCGGAG 
GGCCGCTTCT TCCACCGGGA GTGTTTCCGC TGCAGCGTCT GCGCCACCAC CTTGCACCTG 
GCCACCTACG CCTTCGACGT TGACGAAGGC AAGTTTTTCT GCAAGCCTCA TTTCATTCAC 
TGTAAGACCA ACAGCCAGCA ACGGAAGAGA CGGGCAGAGT TGAAGCAACA AAAAGAGGAG 
GAGGGGATGT GGAAAGAGCA AGAAGCCGCT AGGCGAGACC CTCCCTCGGA AAGTTCTTGC 
GCTGCCGCTG CCATCGCCAC GCCGGAAGGC AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG 
AAGCCCAAGA GCATCCCGGA GCCTGAGCCT GGGGATGTAG CAGGTGGAGC CGCCTCCCCC 
CTGCCCTCTG AGTGGACCGC AGTGAGAATC AGCCCCGGGG AGGATGTGGT TGGACAGGAT 
GTGCTGGCCG TACGCGTCCT GGTCACCAGC GATGACAGCA GCTCCGATGC AGAATTGGAC 
CGTGGTGACA GTGAGGCCTC CAGTGTGGAG CCCTTTGATG AAAGACCCCA GCAACCGGAA 
CTCCAGCTCC CACCCCTCCT GAAGCCCTTC ACCCGGCACA GCTCCCTGAG GGAGGCCCTG 
CCCAGGGCAG TCTCTCCACA TGACCTCGGG GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG 
GCCAATAGTT TCCAGTGTCC AACTCCCAGC GAATACCAGA GTGGGAGAAG ATTCCAGTCC 
AATTTCATGC CGGTGAACAG CAAAATGACC GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC 
CCGCCTTCTC CTTCTCCACC CTCTTCTTCC TCGATTGGTG ACCTGAATGC CTTGGACAGT 
CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC ACCGCCTCAC AAATCTCCAG AGGTCACTGC 
AGTCCATCCA CCCCCATCTT TCTGAGGCGT GCCAAAGCCC ATGGACCACC CAAGGATCTC 
CCCCTGCGTC TGCCTCGTGG GCAGGTACTG GAGCGGACAG AATACTGCCT GGTGAGGCCT 
GGTGGAAATG GCCTCCAGAG CTCCAGGCCA CCCAGCCCTA CAGAGATGGC TTCTGATGGG 
TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC ATCAGAAGCA TCCACAGAGG CCCACACCCC 
GTTGAAGGGA AGGACAGGTG CTTGCCAGAC CACCCACTGT CTCCCTGGGC TGGAGAGGAG 
CCTGGAGAAG GGAGTACCAG ACCCAGGAGG GTAGAGGATG GTGGGTTAGA GCTGGCAGAG 
GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC ACTCCGGAGC AGAAGACTTG GTTGTTGGAT 
TGGAACGACT CCAACCTTGA GAACTTGTAC CTGGAAACCG GAGCACGACT TTCCCAAAAG 
AGCACTAGAA ATGGTTCAGG AGGCCATGTG CTGAAACCAG TCCACCCCTT GCTGCTTCCT 
CTGGCAGAGA AAGAGACCTT GCCAGCCCAG AGACAGTCTC AGGAGAAGAT GGGGGCCCCA 
GCTGAACGGG CTCCTGGGGA ACGAAGCATG GCCCCACCCA AGTCTCCACT GCGGCTTATA 
GCCAACGCCA TCCGGAGATC CCTGGAGCCG CTCCTCCCCA ACTCTGACAG TGGGAGGAAG 
GCCTGGGCCA AGCCAGAGTC AAAGACTTTG CCCACCAGCC CACCTCATGC TTACACTTGC 
TCATTCAGCT TTCGGAAAAG CAGCTCCAGT AAAGACAGCG ATCAGCCATC CCCCAAGAGA 
GACGTGGCAA GCAAGGCCTC AGCCTTCTTC TCCCTGGGCT CCCCGACTGC AAGGACTGCC 
CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC GCCCTTTGCA CTCACAGTTT GCCCAGCCGG 
TCATCCAAGG TGTTTCCTGC ACTTGTGCCC CCACCCTGTG GCAAGATGGA AGATGTCCCC 
ACACTCCTGG AGAAAGTGAG TTTGCAGGAG ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG 
AAAAGGACCT CACTCTTCTC CTCCTTCAGA CTCAAAGACA AATCTTTCGA GAGTTCCCTC 
CAAGAATCTG GACCAAGAAA GGACTCCCAA GACCTCTTCA GCAGCCCCAA GGGGAAGGTG 
CAGCCCATGG GCAATGCCCA GCCCCCAGAG AAGCAGGGGC AGCCAATCAG TAGCACCTGC 
CTGGGGCAGA GGGTCCATCC TGTTTCTCCA GAGAAAGATG CAAGTCCCAC TCACGTCCCC 
ATCAGAGCGC AGGGGACAGG GACTGTCTCT TCCACCTCTT CCTCCACCAC CTCCTCTGCA 
GATGAGGAAT TCCATCCCCA GCCTTCCTCG CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA 
AGAAGGAAGC TGGAAAAAGC AACAAAGCAA TTGGTTAAGC AAGAAGAATT GAAAAGACTT 
CATAAAGCTC AGGCCATCCA GAGGCAGTTG GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC 
GAGATCCAGG GCGTGAGGTG GGAGAAGGCG CTGAGAGGAG AAGCAGCAGA TTCAGGCACA 
CAGGATGAAG CACAGCTTTT GCAGGAATGG CTTAAGCTGG TTCTGGAGAA GAATAAATTA 
ATGCGATATG AGTCAGAGCT CCTGATCATG GCCCACGAAC TAGAACTGGA AGATCATCAA 
AGCAGACTAG AGCAGAAATT AAGAGAGAAA ATGCTCCAGG AAGAGAGCCA GAAAGATGAG 
AATGATCTGA ATGAAGAGCA AGAAATACTC ACCGAGATGA TGCAAGTGAT TGAGCAAAGG 
GACAAATTGG TGGATTCCTT AGAGGAACAA CGCATCAGAG AGAAGGCCGA AGACCAGCAC 
TTTGAAAGCT TCATATTCTC TAGGGGCTGT CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015474699.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330185.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.

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CloneID	OBa92668
Clone ID Related Accession (Same CDS sequence)	XM_015466502.1 , XM_015474699.1
Accession Version	XM_015474699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GGAGTCCGTT CGGAAGACGT TTCCCCTGAA CGTGGGTGGC 
AGCGACACCT GTTACTTCTG CAAGAAGCGC GTGTACGTCA TGGAGCGGCT GAGCGCGGAG 
GGCCGCTTCT TCCACCGGGA GTGTTTCCGC TGCAGCGTCT GCGCCACCAC CTTGCACCTG 
GCCACCTACG CCTTCGACGT TGACGAAGGC AAGTTTTTCT GCAAGCCTCA TTTCATTCAC 
TGTAAGACCA ACAGCCAGCA ACGGAAGAGA CGGGCAGAGT TGAAGCAACA AAAAGAGGAG 
GAGGGGATGT GGAAAGAGCA AGAAGCCGCT AGGCGAGACC CTCCCTCGGA AAGTTCTTGC 
GCTGCCGCTG CCATCGCCAC GCCGGAAGGC AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG 
AAGCCCAAGA GCATCCCGGA GCCTGAGCCT GGGGATGTAG CAGGTGGAGC CGCCTCCCCC 
CTGCCCTCTG AGTGGACCGC AGTGAGAATC AGCCCCGGGG AGGATGTGGT TGGACAGGAT 
GTGCTGGCCG TACGCGTCCT GGTCACCAGC GATGACAGCA GCTCCGATGC AGAATTGGAC 
CGTGGTGACA GTGAGGCCTC CAGTGTGGAG CCCTTTGATG AAAGACCCCA GCAACCGGAA 
CTCCAGCTCC CACCCCTCCT GAAGCCCTTC ACCCGGCACA GCTCCCTGAG GGAGGCCCTG 
CCCAGGGCAG TCTCTCCACA TGACCTCGGG GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG 
GCCAATAGTT TCCAGTGTCC AACTCCCAGC GAATACCAGA GTGGGAGAAG ATTCCAGTCC 
AATTTCATGC CGGTGAACAG CAAAATGACC GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC 
CCGCCTTCTC CTTCTCCACC CTCTTCTTCC TCGATTGGTG ACCTGAATGC CTTGGACAGT 
CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC ACCGCCTCAC AAATCTCCAG AGGTCACTGC 
AGTCCATCCA CCCCCATCTT TCTGAGGCGT GCCAAAGCCC ATGGACCACC CAAGGATCTC 
CCCCTGCGTC TGCCTCGTGG GCAGGTACTG GAGCGGACAG AATACTGCCT GGTGAGGCCT 
GGTGGAAATG GCCTCCAGAG CTCCAGGCCA CCCAGCCCTA CAGAGATGGC TTCTGATGGG 
TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC ATCAGAAGCA TCCACAGAGG CCCACACCCC 
GTTGAAGGGA AGGACAGGTG CTTGCCAGAC CACCCACTGT CTCCCTGGGC TGGAGAGGAG 
CCTGGAGAAG GGAGTACCAG ACCCAGGAGG GTAGAGGATG GTGGGTTAGA GCTGGCAGAG 
GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC ACTCCGGAGC AGAAGACTTG GTTGTTGGAT 
TGGAACGACT CCAACCTTGA GAACTTGTAC CTGGAAACCG GAGCACGACT TTCCCAAAAG 
AGCACTAGAA ATGGTTCAGG AGGCCATGTG CTGAAACCAG TCCACCCCTT GCTGCTTCCT 
CTGGCAGAGA AAGAGACCTT GCCAGCCCAG AGACAGTCTC AGGAGAAGAT GGGGGCCCCA 
GCTGAACGGG CTCCTGGGGA ACGAAGCATG GCCCCACCCA AGTCTCCACT GCGGCTTATA 
GCCAACGCCA TCCGGAGATC CCTGGAGCCG CTCCTCCCCA ACTCTGACAG TGGGAGGAAG 
GCCTGGGCCA AGCCAGAGTC AAAGACTTTG CCCACCAGCC CACCTCATGC TTACACTTGC 
TCATTCAGCT TTCGGAAAAG CAGCTCCAGT AAAGACAGCG ATCAGCCATC CCCCAAGAGA 
GACGTGGCAA GCAAGGCCTC AGCCTTCTTC TCCCTGGGCT CCCCGACTGC AAGGACTGCC 
CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC GCCCTTTGCA CTCACAGTTT GCCCAGCCGG 
TCATCCAAGG TGTTTCCTGC ACTTGTGCCC CCACCCTGTG GCAAGATGGA AGATGTCCCC 
ACACTCCTGG AGAAAGTGAG TTTGCAGGAG ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG 
AAAAGGACCT CACTCTTCTC CTCCTTCAGA CTCAAAGACA AATCTTTCGA GAGTTCCCTC 
CAAGAATCTG GACCAAGAAA GGACTCCCAA GACCTCTTCA GCAGCCCCAA GGGGAAGGTG 
CAGCCCATGG GCAATGCCCA GCCCCCAGAG AAGCAGGGGC AGCCAATCAG TAGCACCTGC 
CTGGGGCAGA GGGTCCATCC TGTTTCTCCA GAGAAAGATG CAAGTCCCAC TCACGTCCCC 
ATCAGAGCGC AGGGGACAGG GACTGTCTCT TCCACCTCTT CCTCCACCAC CTCCTCTGCA 
GATGAGGAAT TCCATCCCCA GCCTTCCTCG CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA 
AGAAGGAAGC TGGAAAAAGC AACAAAGCAA TTGGTTAAGC AAGAAGAATT GAAAAGACTT 
CATAAAGCTC AGGCCATCCA GAGGCAGTTG GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC 
GAGATCCAGG GCGTGAGGTG GGAGAAGGCG CTGAGAGGAG AAGCAGCAGA TTCAGGCACA 
CAGGATGAAG CACAGCTTTT GCAGGAATGG CTTAAGCTGG TTCTGGAGAA GAATAAATTA 
ATGCGATATG AGTCAGAGCT CCTGATCATG GCCCACGAAC TAGAACTGGA AGATCATCAA 
AGCAGACTAG AGCAGAAATT AAGAGAGAAA ATGCTCCAGG AAGAGAGCCA GAAAGATGAG 
AATGATCTGA ATGAAGAGCA AGAAATACTC ACCGAGATGA TGCAAGTGAT TGAGCAAAGG 
GACAAATTGG TGGATTCCTT AGAGGAACAA CGCATCAGAG AGAAGGCCGA AGACCAGCAC 
TTTGAAAGCT TCATATTCTC TAGGGGCTGT CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330185.1
CDS	330..6137
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015474699.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474699.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GGAGTCCGTT CGGAAGACGT TTCCCCTGAA CGTGGGTGGC 
AGCGACACCT GTTACTTCTG CAAGAAGCGC GTGTACGTCA TGGAGCGGCT GAGCGCGGAG 
GGCCGCTTCT TCCACCGGGA GTGTTTCCGC TGCAGCGTCT GCGCCACCAC CTTGCACCTG 
GCCACCTACG CCTTCGACGT TGACGAAGGC AAGTTTTTCT GCAAGCCTCA TTTCATTCAC 
TGTAAGACCA ACAGCCAGCA ACGGAAGAGA CGGGCAGAGT TGAAGCAACA AAAAGAGGAG 
GAGGGGATGT GGAAAGAGCA AGAAGCCGCT AGGCGAGACC CTCCCTCGGA AAGTTCTTGC 
GCTGCCGCTG CCATCGCCAC GCCGGAAGGC AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG 
AAGCCCAAGA GCATCCCGGA GCCTGAGCCT GGGGATGTAG CAGGTGGAGC CGCCTCCCCC 
CTGCCCTCTG AGTGGACCGC AGTGAGAATC AGCCCCGGGG AGGATGTGGT TGGACAGGAT 
GTGCTGGCCG TACGCGTCCT GGTCACCAGC GATGACAGCA GCTCCGATGC AGAATTGGAC 
CGTGGTGACA GTGAGGCCTC CAGTGTGGAG CCCTTTGATG AAAGACCCCA GCAACCGGAA 
CTCCAGCTCC CACCCCTCCT GAAGCCCTTC ACCCGGCACA GCTCCCTGAG GGAGGCCCTG 
CCCAGGGCAG TCTCTCCACA TGACCTCGGG GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG 
GCCAATAGTT TCCAGTGTCC AACTCCCAGC GAATACCAGA GTGGGAGAAG ATTCCAGTCC 
AATTTCATGC CGGTGAACAG CAAAATGACC GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC 
CCGCCTTCTC CTTCTCCACC CTCTTCTTCC TCGATTGGTG ACCTGAATGC CTTGGACAGT 
CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC ACCGCCTCAC AAATCTCCAG AGGTCACTGC 
AGTCCATCCA CCCCCATCTT TCTGAGGCGT GCCAAAGCCC ATGGACCACC CAAGGATCTC 
CCCCTGCGTC TGCCTCGTGG GCAGGTACTG GAGCGGACAG AATACTGCCT GGTGAGGCCT 
GGTGGAAATG GCCTCCAGAG CTCCAGGCCA CCCAGCCCTA CAGAGATGGC TTCTGATGGG 
TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC ATCAGAAGCA TCCACAGAGG CCCACACCCC 
GTTGAAGGGA AGGACAGGTG CTTGCCAGAC CACCCACTGT CTCCCTGGGC TGGAGAGGAG 
CCTGGAGAAG GGAGTACCAG ACCCAGGAGG GTAGAGGATG GTGGGTTAGA GCTGGCAGAG 
GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC ACTCCGGAGC AGAAGACTTG GTTGTTGGAT 
TGGAACGACT CCAACCTTGA GAACTTGTAC CTGGAAACCG GAGCACGACT TTCCCAAAAG 
AGCACTAGAA ATGGTTCAGG AGGCCATGTG CTGAAACCAG TCCACCCCTT GCTGCTTCCT 
CTGGCAGAGA AAGAGACCTT GCCAGCCCAG AGACAGTCTC AGGAGAAGAT GGGGGCCCCA 
GCTGAACGGG CTCCTGGGGA ACGAAGCATG GCCCCACCCA AGTCTCCACT GCGGCTTATA 
GCCAACGCCA TCCGGAGATC CCTGGAGCCG CTCCTCCCCA ACTCTGACAG TGGGAGGAAG 
GCCTGGGCCA AGCCAGAGTC AAAGACTTTG CCCACCAGCC CACCTCATGC TTACACTTGC 
TCATTCAGCT TTCGGAAAAG CAGCTCCAGT AAAGACAGCG ATCAGCCATC CCCCAAGAGA 
GACGTGGCAA GCAAGGCCTC AGCCTTCTTC TCCCTGGGCT CCCCGACTGC AAGGACTGCC 
CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC GCCCTTTGCA CTCACAGTTT GCCCAGCCGG 
TCATCCAAGG TGTTTCCTGC ACTTGTGCCC CCACCCTGTG GCAAGATGGA AGATGTCCCC 
ACACTCCTGG AGAAAGTGAG TTTGCAGGAG ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG 
AAAAGGACCT CACTCTTCTC CTCCTTCAGA CTCAAAGACA AATCTTTCGA GAGTTCCCTC 
CAAGAATCTG GACCAAGAAA GGACTCCCAA GACCTCTTCA GCAGCCCCAA GGGGAAGGTG 
CAGCCCATGG GCAATGCCCA GCCCCCAGAG AAGCAGGGGC AGCCAATCAG TAGCACCTGC 
CTGGGGCAGA GGGTCCATCC TGTTTCTCCA GAGAAAGATG CAAGTCCCAC TCACGTCCCC 
ATCAGAGCGC AGGGGACAGG GACTGTCTCT TCCACCTCTT CCTCCACCAC CTCCTCTGCA 
GATGAGGAAT TCCATCCCCA GCCTTCCTCG CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA 
AGAAGGAAGC TGGAAAAAGC AACAAAGCAA TTGGTTAAGC AAGAAGAATT GAAAAGACTT 
CATAAAGCTC AGGCCATCCA GAGGCAGTTG GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC 
GAGATCCAGG GCGTGAGGTG GGAGAAGGCG CTGAGAGGAG AAGCAGCAGA TTCAGGCACA 
CAGGATGAAG CACAGCTTTT GCAGGAATGG CTTAAGCTGG TTCTGGAGAA GAATAAATTA 
ATGCGATATG AGTCAGAGCT CCTGATCATG GCCCACGAAC TAGAACTGGA AGATCATCAA 
AGCAGACTAG AGCAGAAATT AAGAGAGAAA ATGCTCCAGG AAGAGAGCCA GAAAGATGAG 
AATGATCTGA ATGAAGAGCA AGAAATACTC ACCGAGATGA TGCAAGTGAT TGAGCAAAGG 
GACAAATTGG TGGATTCCTT AGAGGAACAA CGCATCAGAG AGAAGGCCGA AGACCAGCAC 
TTTGAAAGCT TCATATTCTC TAGGGGCTGT CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466498.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321984.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.

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CloneID	OBa52582
Clone ID Related Accession (Same CDS sequence)	XM_015466498.1 , XM_015466499.1 , XM_010812537.2 , XM_015474697.1
Accession Version	XM_015466498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321984.1
CDS	330..6245
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466498.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466498.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466499.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321985.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.

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CloneID	OBa52582
Clone ID Related Accession (Same CDS sequence)	XM_015466498.1 , XM_015466499.1 , XM_010812537.2 , XM_015474697.1
Accession Version	XM_015466499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321985.1
CDS	326..6241
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466499.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466499.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812537.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810839.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.

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CloneID	OBa52582
Clone ID Related Accession (Same CDS sequence)	XM_015466498.1 , XM_015466499.1 , XM_010812537.2 , XM_015474697.1
Accession Version	XM_010812537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810839.1
CDS	330..6245
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812537.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812537.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015474697.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330183.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.

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CloneID	OBa52582
Clone ID Related Accession (Same CDS sequence)	XM_015466498.1 , XM_015466499.1 , XM_010812537.2 , XM_015474697.1
Accession Version	XM_015474697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330183.1
CDS	326..6241
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015474697.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474697.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGCAGATT CAGGCACACA GGATGAAGCA 
CAGCTTTTGC AGGAATGGCT TAAGCTGGTT CTGGAGAAGA ATAAATTAAT GCGATATGAG 
TCAGAGCTCC TGATCATGGC CCACGAACTA GAACTGGAAG ATCATCAAAG CAGACTAGAG 
CAGAAATTAA GAGAGAAAAT GCTCCAGGAA GAGAGCCAGA AAGATGAGAA TGATCTGAAT 
GAAGAGCAAG AAATACTCAC CGAGATGATG CAAGTGATTG AGCAAAGGGA CAAATTGGTG 
GATTCCTTAG AGGAACAACG CATCAGAGAG AAGGCCGAAG ACCAGCACTT TGAAAGCTTC 
ATATTCTCTA GGGGCTGTCA GCTGAGCAGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010822805.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821107.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52583
Clone ID Related Accession (Same CDS sequence)	XM_010822805.2 , XM_010812538.2
Accession Version	XM_010822805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821107.2
CDS	330..6242
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010822805.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822805.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812538.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810840.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.

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CloneID	OBa52583
Clone ID Related Accession (Same CDS sequence)	XM_010822805.2 , XM_010812538.2
Accession Version	XM_010812538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810840.1
CDS	330..6242
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812538.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812538.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGAATAAACT CCTCTCTAAA AGCCTGTCTC ATACTCATCC TCCGTCTTCT 
CCCTCCTGCC TTCCATCTCC TGATTCAGCT GCTACTTCCT CTCCATCAAC TGTTGACTCT 
GTTTCTCCTG CCAGAAAGGC AAAGAAGTCA CCTTCAGGTT CCCATTTCCA ACCCAGCCAC 
TTAAGAACAT TGCGGCCTCA GCTGACAGTA GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA 
GCTGAAGTTC TGGTCAATCT GTACTTGAAT GATCACAGAC CTAAGACTCA GGCCACCTCT 
CCAGACCTGG AGTCCGTTCG GAAGACGTTT CCCCTGAACG TGGGTGGCAG CGACACCTGT 
TACTTCTGCA AGAAGCGCGT GTACGTCATG GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC 
CACCGGGAGT GTTTCCGCTG CAGCGTCTGC GCCACCACCT TGCACCTGGC CACCTACGCC 
TTCGACGTTG ACGAAGGCAA GTTTTTCTGC AAGCCTCATT TCATTCACTG TAAGACCAAC 
AGCCAGCAAC GGAAGAGACG GGCAGAGTTG AAGCAACAAA AAGAGGAGGA GGGGATGTGG 
AAAGAGCAAG AAGCCGCTAG GCGAGACCCT CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC 
ATCGCCACGC CGGAAGGCAG CCCCCCAGAT GAGCCGACCT CCCCCAAGAA GCCCAAGAGC 
ATCCCGGAGC CTGAGCCTGG GGATGTAGCA GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG 
TGGACCGCAG TGAGAATCAG CCCCGGGGAG GATGTGGTTG GACAGGATGT GCTGGCCGTA 
CGCGTCCTGG TCACCAGCGA TGACAGCAGC TCCGATGCAG AATTGGACCG TGGTGACAGT 
GAGGCCTCCA GTGTGGAGCC CTTTGATGAA AGACCCCAGC AACCGGAACT CCAGCTCCCA 
CCCCTCCTGA AGCCCTTCAC CCGGCACAGC TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC 
TCTCCACATG ACCTCGGGGA GCCCGAAGCT GAGCCTGCTC TGCAGCGGGC CAATAGTTTC 
CAGTGTCCAA CTCCCAGCGA ATACCAGAGT GGGAGAAGAT TCCAGTCCAA TTTCATGCCG 
GTGAACAGCA AAATGACCGT GTCGCCTCCA AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT 
TCTCCACCCT CTTCTTCCTC GATTGGTGAC CTGAATGCCT TGGACAGTCC CTCCCTGCCT 
CAGGTGACAG TTTCCAACAC CGCCTCACAA ATCTCCAGAG GTCACTGCAG TCCATCCACC 
CCCATCTTTC TGAGGCGTGC CAAAGCCCAT GGACCACCCA AGGATCTCCC CCTGCGTCTG 
CCTCGTGGGC AGGTACTGGA GCGGACAGAA TACTGCCTGG TGAGGCCTGG TGGAAATGGC 
CTCCAGAGCT CCAGGCCACC CAGCCCTACA GAGATGGCTT CTGATGGGTG TCAGGAGGCA 
GTGGCCCCTC CTAGGGACAT CAGAAGCATC CACAGAGGCC CACACCCCGT TGAAGGGAAG 
GACAGGTGCT TGCCAGACCA CCCACTGTCT CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG 
AGTACCAGAC CCAGGAGGGT AGAGGATGGT GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC 
TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG AAGACTTGGT TGTTGGATTG GAACGACTCC 
AACCTTGAGA ACTTGTACCT GGAAACCGGA GCACGACTTT CCCAAAAGAG CACTAGAAAT 
GGTTCAGGAG GCCATGTGCT GAAACCAGTC CACCCCTTGC TGCTTCCTCT GGCAGAGAAA 
GAGACCTTGC CAGCCCAGAG ACAGTCTCAG GAGAAGATGG GGGCCCCAGC TGAACGGGCT 
CCTGGGGAAC GAAGCATGGC CCCACCCAAG TCTCCACTGC GGCTTATAGC CAACGCCATC 
CGGAGATCCC TGGAGCCGCT CCTCCCCAAC TCTGACAGTG GGAGGAAGGC CTGGGCCAAG 
CCAGAGTCAA AGACTTTGCC CACCAGCCCA CCTCATGCTT ACACTTGCTC ATTCAGCTTT 
CGGAAAAGCA GCTCCAGTAA AGACAGCGAT CAGCCATCCC CCAAGAGAGA CGTGGCAAGC 
AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC CCGACTGCAA GGACTGCCCA GCCCTCAGCC 
CCGAGCCCTC CTGCCCCCGC CCTTTGCACT CACAGTTTGC CCAGCCGGTC ATCCAAGGTG 
TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC AAGATGGAAG ATGTCCCCAC ACTCCTGGAG 
AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT GCTTCCAGGG TTCCCAAGAA AAGGACCTCA 
CTCTTCTCCT CCTTCAGACT CAAAGACAAA TCTTTCGAGA GTTCCCTCCA AGAATCTGGA 
CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC AGCCCCAAGG GGAAGGTGCA GCCCATGGGC 
AATGCCCAGC CCCCAGAGAA GCAGGGGCAG CCAATCAGTA GCACCTGCCT GGGGCAGAGG 
GTCCATCCTG TTTCTCCAGA GAAAGATGCA AGTCCCACTC ACGTCCCCAT CAGAGCGCAG 
GGGACAGGGA CTGTCTCTTC CACCTCTTCC TCCACCACCT CCTCTGCAGA TGAGGAATTC 
CATCCCCAGC CTTCCTCGCG GTCAAAGGAG AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG 
GAAAAAGCAA CAAAGCAATT GGTTAAGCAA GAAGAATTGA AAAGACTTCA TAAAGCTCAG 
GCCATCCAGA GGCAGTTGGA GGAAGTGGAG GAGCAGCAGA GGGCTTTCGA GATCCAGGGC 
GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA GCAGATTCAG GCACACAGGA TGAAGCACAG 
CTTTTGCAGG AATGGCTTAA GCTGGTTCTG GAGAAGAATA AATTAATGCG ATATGAGTCA 
GAGCTCCTGA TCATGGCCCA CGAACTAGAA CTGGAAGATC ATCAAAGCAG ACTAGAGCAG 
AAATTAAGAG AGAAAATGCT CCAGGAAGAG AGCCAGAAAG ATGAGAATGA TCTGAATGAA 
GAGCAAGAAA TACTCACCGA GATGATGCAA GTGATTGAGC AAAGGGACAA ATTGGTGGAT 
TCCTTAGAGG AACAACGCAT CAGAGAGAAG GCCGAAGACC AGCACTTTGA AAGCTTCATA 
TTCTCTAGGG GCTGTCAGCT GAGCAGGACG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812539.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810841.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.

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CloneID	OBa52584
Clone ID Related Accession (Same CDS sequence)	XM_010812539.2 , XM_015466501.1
Accession Version	XM_010812539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAG GTTCAGAAGA 
AAAGAATTCC GTGGGAAGCT GGCTATTGCA ATCACCGCCA ACTTCATCAA CAGAAACAGC 
ACAGCTGAGG CTAAGGTGGA AGAGATTAGC GGTGTGGCTT TCATCTTCAA TCAGAAATTT 
TTTCAGGATC TTAAAAAAGA AACAGGGATT GATCTTGAGA ACATTGTTTA CTACAAGGAC 
TGCACCCACT ATTTTGTCAT GACTGCCAAG AGGCAGAGCT TGCTTGACAA AGGTGTCATC 
ATTAATGACT ATACCGACAC AGAGACGCTG CTGCGCGCGG AGAATGTGAA CCAGGACAAC 
CTGCTTTCCT ACGCACGCGA GGCTGCAGAC TTCGCCACCA ATTACCAGCT GCCGTCTTTA 
GACTTTGCCA TGAACCATTA CGGGCAGCCC GACGTGGCCA TGTTCGACTT CACCTCCATG 
TACGCCTCCG AGAATGCGGC CTTGGTGCGC GAGCGGCAGT CACACCTGCT GCTCGTGGCC 
CTCGTGGGTG ACAGCTTGCT TGAGCCATTC TGGCCCATGG GCACTGGCTG TGCCCGTGGC 
TTCCTGGCAG CCTTTGACAC TGCATGGATG GTGAAAAGCT GGGACCAGGG CACTTCTCCC 
CTGGAGCTTC TGGCTGAAAG AGAAAGTCTT TACCGACTGT TACCTCAAAC GACCCCAGAG 
AACATCAACA AGAACTTTGA GCAGTACACA TTAGACCCGG CGACACGGTA CCCAAACCTC 
AACTCAAACA ACATCAAGCC GAATCAGGTG AAGCATTTGT ACATTACTAA GGAGCTGCAC 
CAGTATCCTC TTGAGAGGCT GGGCTCAGTG AGGAGATCCG TCGGCCTCTC CAGGCGAGAG 
TCAGACGTCA GGCCCAGCAA GCTCTTAACC TGGTGCCAGC AGCAGACCGA GGGCTACCAG 
CATGTCAATG TCACCGACCT GACCACGTCC TGGCGGAGTG GCCTGGCCCT GTGTGCCATC 
ATCCACCGCT TCCGGCCTGA CCTGATCAAC TTTGACTCTT TGAATGAAGA CCACGCTGTG 
GAGAACAACC AGCTGGCATT TGACGTGGCG GAGCGTGAGT TTGGCATCGC CCCAGTGATC 
ACAGGCAAAG AGATGGCATC GGCCCAGGAG CCTGACAAGC TCAGTATGGT CATGTACCTC 
TCCAAGTTCT ACGAGCTCTT CCGGGGCACC CCTCTGAGGC CTGTGGATTC TTGGCGTAAA 
AACTATGAAG AGAATGCCGA CCTCGGCTTG GCCAAGTCAT CCATTCCTCA TCACTATCTC 
AACCTCACGT TTCCGAGGAA GAGGACTCCG CGAGTGGACA GTCGAACCGA AGAGAATGAC 
ATGAACAAGC GGAGGCGGAA GGGCTTCAAC AACCTGCACG AGCCTTCAGC CTTTTCCAGC 
CAGAGCTTGG GCTCCAATCA AGAGGGCATT AAGGAAGGTG GGAATCAGAA CAAAGTCAAG 
TCCATGGCAA GTCAGCTGCT GGCCAAGTTC GAGGAGAGCT CACGGAACCC CTCAATCCTG 
AGGCAGGAGC ACCGTGTCTC AGGGATAGGT AAGCCTATAC TGCGCTCTTC CTCTGACCCT 
CCTGTTAACT CTCGCTGTCC CAAGCCGGAG GAGCCCACAC CCAGCCCATC ACCACCTCTG 
AAAAGGCAGT TCCCCTCCGT GGTTGTGACA GGACACGTGC TCCGAGAGCT AAAGCAAGTG 
TCTGCTGGTG GCGAGTGTCC AGCCAGGCCC TGGAGAGCCC GAGCCAAGTC TGACCTGCAG 
TTGGGCGGGC CAGAAAACCT CGCCAGCCTG CCTCCCACCT GCCCGGGAGC GCTGGCTCTC 
TCTGGGGTGC TGCGGCGGCT GCAGCAAGTG GAGGAAAAGG TTCTCCAGAA AAGGGCCCAG 
AACTTGGCCA ACAGGGAATT TCACAAAAAG AACATTAAGG AGAAGGCGGC TCACCTTGCC 
TCCATGTTTG GACACGGGGA TTTCCCACAG AATAAACTCC TCTCTAAAAG CCTGTCTCAT 
ACTCATCCTC CGTCTTCTCC CTCCTGCCTT CCATCTCCTG ATTCAGCTGC TACTTCCTCT 
CCATCAACTG TTGACTCTGT TTCTCCTGCC AGAAAGGCAA AGAAGTCACC TTCAGGTTCC 
CATTTCCAAC CCAGCCACTT AAGAACATTG CGGCCTCAGC TGACAGTAGG GAAAGTATCC 
AGCGGAATAG GGGCTGCAGC TGAAGTTCTG GTCAATCTGT ACTTGAATGA TCACAGACCT 
AAGACTCAGG CCACCTCTCC AGACCTGGAG TCCGTTCGGA AGACGTTTCC CCTGAACGTG 
GGTGGCAGCG ACACCTGTTA CTTCTGCAAG AAGCGCGTGT ACGTCATGGA GCGGCTGAGC 
GCGGAGGGCC GCTTCTTCCA CCGGGAGTGT TTCCGCTGCA GCGTCTGCGC CACCACCTTG 
CACCTGGCCA CCTACGCCTT CGACGTTGAC GAAGGCAAGT TTTTCTGCAA GCCTCATTTC 
ATTCACTGTA AGACCAACAG CCAGCAACGG AAGAGACGGG CAGAGTTGAA GCAACAAAAA 
GAGGAGGAGG GGATGTGGAA AGAGCAAGAA GCCGCTAGGC GAGACCCTCC CTCGGAAAGT 
TCTTGCGCTG CCGCTGCCAT CGCCACGCCG GAAGGCAGCC CCCCAGATGA GCCGACCTCC 
CCCAAGAAGC CCAAGAGCAT CCCGGAGCCT GAGCCTGGGG ATGTAGCAGG TGGAGCCGCC 
TCCCCCCTGC CCTCTGAGTG GACCGCAGTG AGAATCAGCC CCGGGGAGGA TGTGGTTGGA 
CAGGATGTGC TGGCCGTACG CGTCCTGGTC ACCAGCGATG ACAGCAGCTC CGATGCAGAA 
TTGGACCGTG GTGACAGTGA GGCCTCCAGT GTGGAGCCCT TTGATGAAAG ACCCCAGCAA 
CCGGAACTCC AGCTCCCACC CCTCCTGAAG CCCTTCACCC GGCACAGCTC CCTGAGGGAG 
GCCCTGCCCA GGGCAGTCTC TCCACATGAC CTCGGGGAGC CCGAAGCTGA GCCTGCTCTG 
CAGCGGGCCA ATAGTTTCCA GTGTCCAACT CCCAGCGAAT ACCAGAGTGG GAGAAGATTC 
CAGTCCAATT TCATGCCGGT GAACAGCAAA ATGACCGTGT CGCCTCCAAA GCAAGCTTGT 
CCTCCCCCGC CTTCTCCTTC TCCACCCTCT TCTTCCTCGA TTGGTGACCT GAATGCCTTG 
GACAGTCCCT CCCTGCCTCA GGTGACAGTT TCCAACACCG CCTCACAAAT CTCCAGAGGT 
CACTGCAGTC CATCCACCCC CATCTTTCTG AGGCGTGCCA AAGCCCATGG ACCACCCAAG 
GATCTCCCCC TGCGTCTGCC TCGTGGGCAG GTACTGGAGC GGACAGAATA CTGCCTGGTG 
AGGCCTGGTG GAAATGGCCT CCAGAGCTCC AGGCCACCCA GCCCTACAGA GATGGCTTCT 
GATGGGTGTC AGGAGGCAGT GGCCCCTCCT AGGGACATCA GAAGCATCCA CAGAGGCCCA 
CACCCCGTTG AAGGGAAGGA CAGGTGCTTG CCAGACCACC CACTGTCTCC CTGGGCTGGA 
GAGGAGCCTG GAGAAGGGAG TACCAGACCC AGGAGGGTAG AGGATGGTGG GTTAGAGCTG 
GCAGAGGAGA AGTCAGGCTT AAAGAAGTTG GTTCTCACTC CGGAGCAGAA GACTTGGTTG 
TTGGATTGGA ACGACTCCAA CCTTGAGAAC TTGTACCTGG AAACCGGAGC ACGACTTTCC 
CAAAAGAGCA CTAGAAATGG TTCAGGAGGC CATGTGCTGA AACCAGTCCA CCCCTTGCTG 
CTTCCTCTGG CAGAGAAAGA GACCTTGCCA GCCCAGAGAC AGTCTCAGGA GAAGATGGGG 
GCCCCAGCTG AACGGGCTCC TGGGGAACGA AGCATGGCCC CACCCAAGTC TCCACTGCGG 
CTTATAGCCA ACGCCATCCG GAGATCCCTG GAGCCGCTCC TCCCCAACTC TGACAGTGGG 
AGGAAGGCCT GGGCCAAGCC AGAGTCAAAG ACTTTGCCCA CCAGCCCACC TCATGCTTAC 
ACTTGCTCAT TCAGCTTTCG GAAAAGCAGC TCCAGTAAAG ACAGCGATCA GCCATCCCCC 
AAGAGAGACG TGGCAAGCAA GGCCTCAGCC TTCTTCTCCC TGGGCTCCCC GACTGCAAGG 
ACTGCCCAGC CCTCAGCCCC GAGCCCTCCT GCCCCCGCCC TTTGCACTCA CAGTTTGCCC 
AGCCGGTCAT CCAAGGTGTT TCCTGCACTT GTGCCCCCAC CCTGTGGCAA GATGGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGAGACAG TCCCAGATGC TTCCAGGGTT 
CCCAAGAAAA GGACCTCACT CTTCTCCTCC TTCAGACTCA AAGACAAATC TTTCGAGAGT 
TCCCTCCAAG AATCTGGACC AAGAAAGGAC TCCCAAGACC TCTTCAGCAG CCCCAAGGGG 
AAGGTGCAGC CCATGGGCAA TGCCCAGCCC CCAGAGAAGC AGGGGCAGCC AATCAGTAGC 
ACCTGCCTGG GGCAGAGGGT CCATCCTGTT TCTCCAGAGA AAGATGCAAG TCCCACTCAC 
GTCCCCATCA GAGCGCAGGG GACAGGGACT GTCTCTTCCA CCTCTTCCTC CACCACCTCC 
TCTGCAGATG AGGAATTCCA TCCCCAGCCT TCCTCGCGGT CAAAGGAGAG GAAGGCACTT 
AGAAGAAGAA GGAAGCTGGA AAAAGCAACA AAGCAATTGG TTAAGCAAGA AGAATTGAAA 
AGACTTCATA AAGCTCAGGC CATCCAGAGG CAGTTGGAGG AAGTGGAGGA GCAGCAGAGG 
GCTTTCGAGA TCCAGGGCGT GAGGTGGGAG AAGGCGCTGA GAGGAGAAGC AGCAGATTCA 
GGCACACAGG ATGAAGCACA GCTTTTGCAG GAATGGCTTA AGCTGGTTCT GGAGAAGAAT 
AAATTAATGC GATATGAGTC AGAGCTCCTG ATCATGGCCC ACGAACTAGA ACTGGAAGAT 
CATCAAAGCA GACTAGAGCA GAAATTAAGA GAGAAAATGC TCCAGGAAGA GAGCCAGAAA 
GATGAGAATG ATCTGAATGA AGAGCAAGAA ATACTCACCG AGATGATGCA AGTGATTGAG 
CAAAGGGACA AATTGGTGGA TTCCTTAGAG GAACAACGCA TCAGAGAGAA GGCCGAAGAC 
CAGCACTTTG AAAGCTTCAT ATTCTCTAGG GGCTGTCAGC TGAGCAGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810841.1
CDS	330..6143
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQRFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812539.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812539.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAG GTTCAGAAGA 
AAAGAATTCC GTGGGAAGCT GGCTATTGCA ATCACCGCCA ACTTCATCAA CAGAAACAGC 
ACAGCTGAGG CTAAGGTGGA AGAGATTAGC GGTGTGGCTT TCATCTTCAA TCAGAAATTT 
TTTCAGGATC TTAAAAAAGA AACAGGGATT GATCTTGAGA ACATTGTTTA CTACAAGGAC 
TGCACCCACT ATTTTGTCAT GACTGCCAAG AGGCAGAGCT TGCTTGACAA AGGTGTCATC 
ATTAATGACT ATACCGACAC AGAGACGCTG CTGCGCGCGG AGAATGTGAA CCAGGACAAC 
CTGCTTTCCT ACGCACGCGA GGCTGCAGAC TTCGCCACCA ATTACCAGCT GCCGTCTTTA 
GACTTTGCCA TGAACCATTA CGGGCAGCCC GACGTGGCCA TGTTCGACTT CACCTCCATG 
TACGCCTCCG AGAATGCGGC CTTGGTGCGC GAGCGGCAGT CACACCTGCT GCTCGTGGCC 
CTCGTGGGTG ACAGCTTGCT TGAGCCATTC TGGCCCATGG GCACTGGCTG TGCCCGTGGC 
TTCCTGGCAG CCTTTGACAC TGCATGGATG GTGAAAAGCT GGGACCAGGG CACTTCTCCC 
CTGGAGCTTC TGGCTGAAAG AGAAAGTCTT TACCGACTGT TACCTCAAAC GACCCCAGAG 
AACATCAACA AGAACTTTGA GCAGTACACA TTAGACCCGG CGACACGGTA CCCAAACCTC 
AACTCAAACA ACATCAAGCC GAATCAGGTG AAGCATTTGT ACATTACTAA GGAGCTGCAC 
CAGTATCCTC TTGAGAGGCT GGGCTCAGTG AGGAGATCCG TCGGCCTCTC CAGGCGAGAG 
TCAGACGTCA GGCCCAGCAA GCTCTTAACC TGGTGCCAGC AGCAGACCGA GGGCTACCAG 
CATGTCAATG TCACCGACCT GACCACGTCC TGGCGGAGTG GCCTGGCCCT GTGTGCCATC 
ATCCACCGCT TCCGGCCTGA CCTGATCAAC TTTGACTCTT TGAATGAAGA CCACGCTGTG 
GAGAACAACC AGCTGGCATT TGACGTGGCG GAGCGTGAGT TTGGCATCGC CCCAGTGATC 
ACAGGCAAAG AGATGGCATC GGCCCAGGAG CCTGACAAGC TCAGTATGGT CATGTACCTC 
TCCAAGTTCT ACGAGCTCTT CCGGGGCACC CCTCTGAGGC CTGTGGATTC TTGGCGTAAA 
AACTATGAAG AGAATGCCGA CCTCGGCTTG GCCAAGTCAT CCATTCCTCA TCACTATCTC 
AACCTCACGT TTCCGAGGAA GAGGACTCCG CGAGTGGACA GTCGAACCGA AGAGAATGAC 
ATGAACAAGC GGAGGCGGAA GGGCTTCAAC AACCTGCACG AGCCTTCAGC CTTTTCCAGC 
CAGAGCTTGG GCTCCAATCA AGAGGGCATT AAGGAAGGTG GGAATCAGAA CAAAGTCAAG 
TCCATGGCAA GTCAGCTGCT GGCCAAGTTC GAGGAGAGCT CACGGAACCC CTCAATCCTG 
AGGCAGGAGC ACCGTGTCTC AGGGATAGGT AAGCCTATAC TGCGCTCTTC CTCTGACCCT 
CCTGTTAACT CTCGCTGTCC CAAGCCGGAG GAGCCCACAC CCAGCCCATC ACCACCTCTG 
AAAAGGCAGT TCCCCTCCGT GGTTGTGACA GGACACGTGC TCCGAGAGCT AAAGCAAGTG 
TCTGCTGGTG GCGAGTGTCC AGCCAGGCCC TGGAGAGCCC GAGCCAAGTC TGACCTGCAG 
TTGGGCGGGC CAGAAAACCT CGCCAGCCTG CCTCCCACCT GCCCGGGAGC GCTGGCTCTC 
TCTGGGGTGC TGCGGCGGCT GCAGCAAGTG GAGGAAAAGG TTCTCCAGAA AAGGGCCCAG 
AACTTGGCCA ACAGGGAATT TCACAAAAAG AACATTAAGG AGAAGGCGGC TCACCTTGCC 
TCCATGTTTG GACACGGGGA TTTCCCACAG AATAAACTCC TCTCTAAAAG CCTGTCTCAT 
ACTCATCCTC CGTCTTCTCC CTCCTGCCTT CCATCTCCTG ATTCAGCTGC TACTTCCTCT 
CCATCAACTG TTGACTCTGT TTCTCCTGCC AGAAAGGCAA AGAAGTCACC TTCAGGTTCC 
CATTTCCAAC CCAGCCACTT AAGAACATTG CGGCCTCAGC TGACAGTAGG GAAAGTATCC 
AGCGGAATAG GGGCTGCAGC TGAAGTTCTG GTCAATCTGT ACTTGAATGA TCACAGACCT 
AAGACTCAGG CCACCTCTCC AGACCTGGAG TCCGTTCGGA AGACGTTTCC CCTGAACGTG 
GGTGGCAGCG ACACCTGTTA CTTCTGCAAG AAGCGCGTGT ACGTCATGGA GCGGCTGAGC 
GCGGAGGGCC GCTTCTTCCA CCGGGAGTGT TTCCGCTGCA GCGTCTGCGC CACCACCTTG 
CACCTGGCCA CCTACGCCTT CGACGTTGAC GAAGGCAAGT TTTTCTGCAA GCCTCATTTC 
ATTCACTGTA AGACCAACAG CCAGCAACGG AAGAGACGGG CAGAGTTGAA GCAACAAAAA 
GAGGAGGAGG GGATGTGGAA AGAGCAAGAA GCCGCTAGGC GAGACCCTCC CTCGGAAAGT 
TCTTGCGCTG CCGCTGCCAT CGCCACGCCG GAAGGCAGCC CCCCAGATGA GCCGACCTCC 
CCCAAGAAGC CCAAGAGCAT CCCGGAGCCT GAGCCTGGGG ATGTAGCAGG TGGAGCCGCC 
TCCCCCCTGC CCTCTGAGTG GACCGCAGTG AGAATCAGCC CCGGGGAGGA TGTGGTTGGA 
CAGGATGTGC TGGCCGTACG CGTCCTGGTC ACCAGCGATG ACAGCAGCTC CGATGCAGAA 
TTGGACCGTG GTGACAGTGA GGCCTCCAGT GTGGAGCCCT TTGATGAAAG ACCCCAGCAA 
CCGGAACTCC AGCTCCCACC CCTCCTGAAG CCCTTCACCC GGCACAGCTC CCTGAGGGAG 
GCCCTGCCCA GGGCAGTCTC TCCACATGAC CTCGGGGAGC CCGAAGCTGA GCCTGCTCTG 
CAGCGGGCCA ATAGTTTCCA GTGTCCAACT CCCAGCGAAT ACCAGAGTGG GAGAAGATTC 
CAGTCCAATT TCATGCCGGT GAACAGCAAA ATGACCGTGT CGCCTCCAAA GCAAGCTTGT 
CCTCCCCCGC CTTCTCCTTC TCCACCCTCT TCTTCCTCGA TTGGTGACCT GAATGCCTTG 
GACAGTCCCT CCCTGCCTCA GGTGACAGTT TCCAACACCG CCTCACAAAT CTCCAGAGGT 
CACTGCAGTC CATCCACCCC CATCTTTCTG AGGCGTGCCA AAGCCCATGG ACCACCCAAG 
GATCTCCCCC TGCGTCTGCC TCGTGGGCAG GTACTGGAGC GGACAGAATA CTGCCTGGTG 
AGGCCTGGTG GAAATGGCCT CCAGAGCTCC AGGCCACCCA GCCCTACAGA GATGGCTTCT 
GATGGGTGTC AGGAGGCAGT GGCCCCTCCT AGGGACATCA GAAGCATCCA CAGAGGCCCA 
CACCCCGTTG AAGGGAAGGA CAGGTGCTTG CCAGACCACC CACTGTCTCC CTGGGCTGGA 
GAGGAGCCTG GAGAAGGGAG TACCAGACCC AGGAGGGTAG AGGATGGTGG GTTAGAGCTG 
GCAGAGGAGA AGTCAGGCTT AAAGAAGTTG GTTCTCACTC CGGAGCAGAA GACTTGGTTG 
TTGGATTGGA ACGACTCCAA CCTTGAGAAC TTGTACCTGG AAACCGGAGC ACGACTTTCC 
CAAAAGAGCA CTAGAAATGG TTCAGGAGGC CATGTGCTGA AACCAGTCCA CCCCTTGCTG 
CTTCCTCTGG CAGAGAAAGA GACCTTGCCA GCCCAGAGAC AGTCTCAGGA GAAGATGGGG 
GCCCCAGCTG AACGGGCTCC TGGGGAACGA AGCATGGCCC CACCCAAGTC TCCACTGCGG 
CTTATAGCCA ACGCCATCCG GAGATCCCTG GAGCCGCTCC TCCCCAACTC TGACAGTGGG 
AGGAAGGCCT GGGCCAAGCC AGAGTCAAAG ACTTTGCCCA CCAGCCCACC TCATGCTTAC 
ACTTGCTCAT TCAGCTTTCG GAAAAGCAGC TCCAGTAAAG ACAGCGATCA GCCATCCCCC 
AAGAGAGACG TGGCAAGCAA GGCCTCAGCC TTCTTCTCCC TGGGCTCCCC GACTGCAAGG 
ACTGCCCAGC CCTCAGCCCC GAGCCCTCCT GCCCCCGCCC TTTGCACTCA CAGTTTGCCC 
AGCCGGTCAT CCAAGGTGTT TCCTGCACTT GTGCCCCCAC CCTGTGGCAA GATGGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGAGACAG TCCCAGATGC TTCCAGGGTT 
CCCAAGAAAA GGACCTCACT CTTCTCCTCC TTCAGACTCA AAGACAAATC TTTCGAGAGT 
TCCCTCCAAG AATCTGGACC AAGAAAGGAC TCCCAAGACC TCTTCAGCAG CCCCAAGGGG 
AAGGTGCAGC CCATGGGCAA TGCCCAGCCC CCAGAGAAGC AGGGGCAGCC AATCAGTAGC 
ACCTGCCTGG GGCAGAGGGT CCATCCTGTT TCTCCAGAGA AAGATGCAAG TCCCACTCAC 
GTCCCCATCA GAGCGCAGGG GACAGGGACT GTCTCTTCCA CCTCTTCCTC CACCACCTCC 
TCTGCAGATG AGGAATTCCA TCCCCAGCCT TCCTCGCGGT CAAAGGAGAG GAAGGCACTT 
AGAAGAAGAA GGAAGCTGGA AAAAGCAACA AAGCAATTGG TTAAGCAAGA AGAATTGAAA 
AGACTTCATA AAGCTCAGGC CATCCAGAGG CAGTTGGAGG AAGTGGAGGA GCAGCAGAGG 
GCTTTCGAGA TCCAGGGCGT GAGGTGGGAG AAGGCGCTGA GAGGAGAAGC AGCAGATTCA 
GGCACACAGG ATGAAGCACA GCTTTTGCAG GAATGGCTTA AGCTGGTTCT GGAGAAGAAT 
AAATTAATGC GATATGAGTC AGAGCTCCTG ATCATGGCCC ACGAACTAGA ACTGGAAGAT 
CATCAAAGCA GACTAGAGCA GAAATTAAGA GAGAAAATGC TCCAGGAAGA GAGCCAGAAA 
GATGAGAATG ATCTGAATGA AGAGCAAGAA ATACTCACCG AGATGATGCA AGTGATTGAG 
CAAAGGGACA AATTGGTGGA TTCCTTAGAG GAACAACGCA TCAGAGAGAA GGCCGAAGAC 
CAGCACTTTG AAAGCTTCAT ATTCTCTAGG GGCTGTCAGC TGAGCAGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466501.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321987.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.

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CloneID	OBa52584
Clone ID Related Accession (Same CDS sequence)	XM_010812539.2 , XM_015466501.1
Accession Version	XM_015466501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAG GTTCAGAAGA 
AAAGAATTCC GTGGGAAGCT GGCTATTGCA ATCACCGCCA ACTTCATCAA CAGAAACAGC 
ACAGCTGAGG CTAAGGTGGA AGAGATTAGC GGTGTGGCTT TCATCTTCAA TCAGAAATTT 
TTTCAGGATC TTAAAAAAGA AACAGGGATT GATCTTGAGA ACATTGTTTA CTACAAGGAC 
TGCACCCACT ATTTTGTCAT GACTGCCAAG AGGCAGAGCT TGCTTGACAA AGGTGTCATC 
ATTAATGACT ATACCGACAC AGAGACGCTG CTGCGCGCGG AGAATGTGAA CCAGGACAAC 
CTGCTTTCCT ACGCACGCGA GGCTGCAGAC TTCGCCACCA ATTACCAGCT GCCGTCTTTA 
GACTTTGCCA TGAACCATTA CGGGCAGCCC GACGTGGCCA TGTTCGACTT CACCTCCATG 
TACGCCTCCG AGAATGCGGC CTTGGTGCGC GAGCGGCAGT CACACCTGCT GCTCGTGGCC 
CTCGTGGGTG ACAGCTTGCT TGAGCCATTC TGGCCCATGG GCACTGGCTG TGCCCGTGGC 
TTCCTGGCAG CCTTTGACAC TGCATGGATG GTGAAAAGCT GGGACCAGGG CACTTCTCCC 
CTGGAGCTTC TGGCTGAAAG AGAAAGTCTT TACCGACTGT TACCTCAAAC GACCCCAGAG 
AACATCAACA AGAACTTTGA GCAGTACACA TTAGACCCGG CGACACGGTA CCCAAACCTC 
AACTCAAACA ACATCAAGCC GAATCAGGTG AAGCATTTGT ACATTACTAA GGAGCTGCAC 
CAGTATCCTC TTGAGAGGCT GGGCTCAGTG AGGAGATCCG TCGGCCTCTC CAGGCGAGAG 
TCAGACGTCA GGCCCAGCAA GCTCTTAACC TGGTGCCAGC AGCAGACCGA GGGCTACCAG 
CATGTCAATG TCACCGACCT GACCACGTCC TGGCGGAGTG GCCTGGCCCT GTGTGCCATC 
ATCCACCGCT TCCGGCCTGA CCTGATCAAC TTTGACTCTT TGAATGAAGA CCACGCTGTG 
GAGAACAACC AGCTGGCATT TGACGTGGCG GAGCGTGAGT TTGGCATCGC CCCAGTGATC 
ACAGGCAAAG AGATGGCATC GGCCCAGGAG CCTGACAAGC TCAGTATGGT CATGTACCTC 
TCCAAGTTCT ACGAGCTCTT CCGGGGCACC CCTCTGAGGC CTGTGGATTC TTGGCGTAAA 
AACTATGAAG AGAATGCCGA CCTCGGCTTG GCCAAGTCAT CCATTCCTCA TCACTATCTC 
AACCTCACGT TTCCGAGGAA GAGGACTCCG CGAGTGGACA GTCGAACCGA AGAGAATGAC 
ATGAACAAGC GGAGGCGGAA GGGCTTCAAC AACCTGCACG AGCCTTCAGC CTTTTCCAGC 
CAGAGCTTGG GCTCCAATCA AGAGGGCATT AAGGAAGGTG GGAATCAGAA CAAAGTCAAG 
TCCATGGCAA GTCAGCTGCT GGCCAAGTTC GAGGAGAGCT CACGGAACCC CTCAATCCTG 
AGGCAGGAGC ACCGTGTCTC AGGGATAGGT AAGCCTATAC TGCGCTCTTC CTCTGACCCT 
CCTGTTAACT CTCGCTGTCC CAAGCCGGAG GAGCCCACAC CCAGCCCATC ACCACCTCTG 
AAAAGGCAGT TCCCCTCCGT GGTTGTGACA GGACACGTGC TCCGAGAGCT AAAGCAAGTG 
TCTGCTGGTG GCGAGTGTCC AGCCAGGCCC TGGAGAGCCC GAGCCAAGTC TGACCTGCAG 
TTGGGCGGGC CAGAAAACCT CGCCAGCCTG CCTCCCACCT GCCCGGGAGC GCTGGCTCTC 
TCTGGGGTGC TGCGGCGGCT GCAGCAAGTG GAGGAAAAGG TTCTCCAGAA AAGGGCCCAG 
AACTTGGCCA ACAGGGAATT TCACAAAAAG AACATTAAGG AGAAGGCGGC TCACCTTGCC 
TCCATGTTTG GACACGGGGA TTTCCCACAG AATAAACTCC TCTCTAAAAG CCTGTCTCAT 
ACTCATCCTC CGTCTTCTCC CTCCTGCCTT CCATCTCCTG ATTCAGCTGC TACTTCCTCT 
CCATCAACTG TTGACTCTGT TTCTCCTGCC AGAAAGGCAA AGAAGTCACC TTCAGGTTCC 
CATTTCCAAC CCAGCCACTT AAGAACATTG CGGCCTCAGC TGACAGTAGG GAAAGTATCC 
AGCGGAATAG GGGCTGCAGC TGAAGTTCTG GTCAATCTGT ACTTGAATGA TCACAGACCT 
AAGACTCAGG CCACCTCTCC AGACCTGGAG TCCGTTCGGA AGACGTTTCC CCTGAACGTG 
GGTGGCAGCG ACACCTGTTA CTTCTGCAAG AAGCGCGTGT ACGTCATGGA GCGGCTGAGC 
GCGGAGGGCC GCTTCTTCCA CCGGGAGTGT TTCCGCTGCA GCGTCTGCGC CACCACCTTG 
CACCTGGCCA CCTACGCCTT CGACGTTGAC GAAGGCAAGT TTTTCTGCAA GCCTCATTTC 
ATTCACTGTA AGACCAACAG CCAGCAACGG AAGAGACGGG CAGAGTTGAA GCAACAAAAA 
GAGGAGGAGG GGATGTGGAA AGAGCAAGAA GCCGCTAGGC GAGACCCTCC CTCGGAAAGT 
TCTTGCGCTG CCGCTGCCAT CGCCACGCCG GAAGGCAGCC CCCCAGATGA GCCGACCTCC 
CCCAAGAAGC CCAAGAGCAT CCCGGAGCCT GAGCCTGGGG ATGTAGCAGG TGGAGCCGCC 
TCCCCCCTGC CCTCTGAGTG GACCGCAGTG AGAATCAGCC CCGGGGAGGA TGTGGTTGGA 
CAGGATGTGC TGGCCGTACG CGTCCTGGTC ACCAGCGATG ACAGCAGCTC CGATGCAGAA 
TTGGACCGTG GTGACAGTGA GGCCTCCAGT GTGGAGCCCT TTGATGAAAG ACCCCAGCAA 
CCGGAACTCC AGCTCCCACC CCTCCTGAAG CCCTTCACCC GGCACAGCTC CCTGAGGGAG 
GCCCTGCCCA GGGCAGTCTC TCCACATGAC CTCGGGGAGC CCGAAGCTGA GCCTGCTCTG 
CAGCGGGCCA ATAGTTTCCA GTGTCCAACT CCCAGCGAAT ACCAGAGTGG GAGAAGATTC 
CAGTCCAATT TCATGCCGGT GAACAGCAAA ATGACCGTGT CGCCTCCAAA GCAAGCTTGT 
CCTCCCCCGC CTTCTCCTTC TCCACCCTCT TCTTCCTCGA TTGGTGACCT GAATGCCTTG 
GACAGTCCCT CCCTGCCTCA GGTGACAGTT TCCAACACCG CCTCACAAAT CTCCAGAGGT 
CACTGCAGTC CATCCACCCC CATCTTTCTG AGGCGTGCCA AAGCCCATGG ACCACCCAAG 
GATCTCCCCC TGCGTCTGCC TCGTGGGCAG GTACTGGAGC GGACAGAATA CTGCCTGGTG 
AGGCCTGGTG GAAATGGCCT CCAGAGCTCC AGGCCACCCA GCCCTACAGA GATGGCTTCT 
GATGGGTGTC AGGAGGCAGT GGCCCCTCCT AGGGACATCA GAAGCATCCA CAGAGGCCCA 
CACCCCGTTG AAGGGAAGGA CAGGTGCTTG CCAGACCACC CACTGTCTCC CTGGGCTGGA 
GAGGAGCCTG GAGAAGGGAG TACCAGACCC AGGAGGGTAG AGGATGGTGG GTTAGAGCTG 
GCAGAGGAGA AGTCAGGCTT AAAGAAGTTG GTTCTCACTC CGGAGCAGAA GACTTGGTTG 
TTGGATTGGA ACGACTCCAA CCTTGAGAAC TTGTACCTGG AAACCGGAGC ACGACTTTCC 
CAAAAGAGCA CTAGAAATGG TTCAGGAGGC CATGTGCTGA AACCAGTCCA CCCCTTGCTG 
CTTCCTCTGG CAGAGAAAGA GACCTTGCCA GCCCAGAGAC AGTCTCAGGA GAAGATGGGG 
GCCCCAGCTG AACGGGCTCC TGGGGAACGA AGCATGGCCC CACCCAAGTC TCCACTGCGG 
CTTATAGCCA ACGCCATCCG GAGATCCCTG GAGCCGCTCC TCCCCAACTC TGACAGTGGG 
AGGAAGGCCT GGGCCAAGCC AGAGTCAAAG ACTTTGCCCA CCAGCCCACC TCATGCTTAC 
ACTTGCTCAT TCAGCTTTCG GAAAAGCAGC TCCAGTAAAG ACAGCGATCA GCCATCCCCC 
AAGAGAGACG TGGCAAGCAA GGCCTCAGCC TTCTTCTCCC TGGGCTCCCC GACTGCAAGG 
ACTGCCCAGC CCTCAGCCCC GAGCCCTCCT GCCCCCGCCC TTTGCACTCA CAGTTTGCCC 
AGCCGGTCAT CCAAGGTGTT TCCTGCACTT GTGCCCCCAC CCTGTGGCAA GATGGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGAGACAG TCCCAGATGC TTCCAGGGTT 
CCCAAGAAAA GGACCTCACT CTTCTCCTCC TTCAGACTCA AAGACAAATC TTTCGAGAGT 
TCCCTCCAAG AATCTGGACC AAGAAAGGAC TCCCAAGACC TCTTCAGCAG CCCCAAGGGG 
AAGGTGCAGC CCATGGGCAA TGCCCAGCCC CCAGAGAAGC AGGGGCAGCC AATCAGTAGC 
ACCTGCCTGG GGCAGAGGGT CCATCCTGTT TCTCCAGAGA AAGATGCAAG TCCCACTCAC 
GTCCCCATCA GAGCGCAGGG GACAGGGACT GTCTCTTCCA CCTCTTCCTC CACCACCTCC 
TCTGCAGATG AGGAATTCCA TCCCCAGCCT TCCTCGCGGT CAAAGGAGAG GAAGGCACTT 
AGAAGAAGAA GGAAGCTGGA AAAAGCAACA AAGCAATTGG TTAAGCAAGA AGAATTGAAA 
AGACTTCATA AAGCTCAGGC CATCCAGAGG CAGTTGGAGG AAGTGGAGGA GCAGCAGAGG 
GCTTTCGAGA TCCAGGGCGT GAGGTGGGAG AAGGCGCTGA GAGGAGAAGC AGCAGATTCA 
GGCACACAGG ATGAAGCACA GCTTTTGCAG GAATGGCTTA AGCTGGTTCT GGAGAAGAAT 
AAATTAATGC GATATGAGTC AGAGCTCCTG ATCATGGCCC ACGAACTAGA ACTGGAAGAT 
CATCAAAGCA GACTAGAGCA GAAATTAAGA GAGAAAATGC TCCAGGAAGA GAGCCAGAAA 
GATGAGAATG ATCTGAATGA AGAGCAAGAA ATACTCACCG AGATGATGCA AGTGATTGAG 
CAAAGGGACA AATTGGTGGA TTCCTTAGAG GAACAACGCA TCAGAGAGAA GGCCGAAGAC 
CAGCACTTTG AAAGCTTCAT ATTCTCTAGG GGCTGTCAGC TGAGCAGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321987.1
CDS	330..6143
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQRFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQNKLLSKSLSHTHPPSSPSCLPSPDSAATSSPSTVDSVSPARKAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466501.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466501.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAG GTTCAGAAGA 
AAAGAATTCC GTGGGAAGCT GGCTATTGCA ATCACCGCCA ACTTCATCAA CAGAAACAGC 
ACAGCTGAGG CTAAGGTGGA AGAGATTAGC GGTGTGGCTT TCATCTTCAA TCAGAAATTT 
TTTCAGGATC TTAAAAAAGA AACAGGGATT GATCTTGAGA ACATTGTTTA CTACAAGGAC 
TGCACCCACT ATTTTGTCAT GACTGCCAAG AGGCAGAGCT TGCTTGACAA AGGTGTCATC 
ATTAATGACT ATACCGACAC AGAGACGCTG CTGCGCGCGG AGAATGTGAA CCAGGACAAC 
CTGCTTTCCT ACGCACGCGA GGCTGCAGAC TTCGCCACCA ATTACCAGCT GCCGTCTTTA 
GACTTTGCCA TGAACCATTA CGGGCAGCCC GACGTGGCCA TGTTCGACTT CACCTCCATG 
TACGCCTCCG AGAATGCGGC CTTGGTGCGC GAGCGGCAGT CACACCTGCT GCTCGTGGCC 
CTCGTGGGTG ACAGCTTGCT TGAGCCATTC TGGCCCATGG GCACTGGCTG TGCCCGTGGC 
TTCCTGGCAG CCTTTGACAC TGCATGGATG GTGAAAAGCT GGGACCAGGG CACTTCTCCC 
CTGGAGCTTC TGGCTGAAAG AGAAAGTCTT TACCGACTGT TACCTCAAAC GACCCCAGAG 
AACATCAACA AGAACTTTGA GCAGTACACA TTAGACCCGG CGACACGGTA CCCAAACCTC 
AACTCAAACA ACATCAAGCC GAATCAGGTG AAGCATTTGT ACATTACTAA GGAGCTGCAC 
CAGTATCCTC TTGAGAGGCT GGGCTCAGTG AGGAGATCCG TCGGCCTCTC CAGGCGAGAG 
TCAGACGTCA GGCCCAGCAA GCTCTTAACC TGGTGCCAGC AGCAGACCGA GGGCTACCAG 
CATGTCAATG TCACCGACCT GACCACGTCC TGGCGGAGTG GCCTGGCCCT GTGTGCCATC 
ATCCACCGCT TCCGGCCTGA CCTGATCAAC TTTGACTCTT TGAATGAAGA CCACGCTGTG 
GAGAACAACC AGCTGGCATT TGACGTGGCG GAGCGTGAGT TTGGCATCGC CCCAGTGATC 
ACAGGCAAAG AGATGGCATC GGCCCAGGAG CCTGACAAGC TCAGTATGGT CATGTACCTC 
TCCAAGTTCT ACGAGCTCTT CCGGGGCACC CCTCTGAGGC CTGTGGATTC TTGGCGTAAA 
AACTATGAAG AGAATGCCGA CCTCGGCTTG GCCAAGTCAT CCATTCCTCA TCACTATCTC 
AACCTCACGT TTCCGAGGAA GAGGACTCCG CGAGTGGACA GTCGAACCGA AGAGAATGAC 
ATGAACAAGC GGAGGCGGAA GGGCTTCAAC AACCTGCACG AGCCTTCAGC CTTTTCCAGC 
CAGAGCTTGG GCTCCAATCA AGAGGGCATT AAGGAAGGTG GGAATCAGAA CAAAGTCAAG 
TCCATGGCAA GTCAGCTGCT GGCCAAGTTC GAGGAGAGCT CACGGAACCC CTCAATCCTG 
AGGCAGGAGC ACCGTGTCTC AGGGATAGGT AAGCCTATAC TGCGCTCTTC CTCTGACCCT 
CCTGTTAACT CTCGCTGTCC CAAGCCGGAG GAGCCCACAC CCAGCCCATC ACCACCTCTG 
AAAAGGCAGT TCCCCTCCGT GGTTGTGACA GGACACGTGC TCCGAGAGCT AAAGCAAGTG 
TCTGCTGGTG GCGAGTGTCC AGCCAGGCCC TGGAGAGCCC GAGCCAAGTC TGACCTGCAG 
TTGGGCGGGC CAGAAAACCT CGCCAGCCTG CCTCCCACCT GCCCGGGAGC GCTGGCTCTC 
TCTGGGGTGC TGCGGCGGCT GCAGCAAGTG GAGGAAAAGG TTCTCCAGAA AAGGGCCCAG 
AACTTGGCCA ACAGGGAATT TCACAAAAAG AACATTAAGG AGAAGGCGGC TCACCTTGCC 
TCCATGTTTG GACACGGGGA TTTCCCACAG AATAAACTCC TCTCTAAAAG CCTGTCTCAT 
ACTCATCCTC CGTCTTCTCC CTCCTGCCTT CCATCTCCTG ATTCAGCTGC TACTTCCTCT 
CCATCAACTG TTGACTCTGT TTCTCCTGCC AGAAAGGCAA AGAAGTCACC TTCAGGTTCC 
CATTTCCAAC CCAGCCACTT AAGAACATTG CGGCCTCAGC TGACAGTAGG GAAAGTATCC 
AGCGGAATAG GGGCTGCAGC TGAAGTTCTG GTCAATCTGT ACTTGAATGA TCACAGACCT 
AAGACTCAGG CCACCTCTCC AGACCTGGAG TCCGTTCGGA AGACGTTTCC CCTGAACGTG 
GGTGGCAGCG ACACCTGTTA CTTCTGCAAG AAGCGCGTGT ACGTCATGGA GCGGCTGAGC 
GCGGAGGGCC GCTTCTTCCA CCGGGAGTGT TTCCGCTGCA GCGTCTGCGC CACCACCTTG 
CACCTGGCCA CCTACGCCTT CGACGTTGAC GAAGGCAAGT TTTTCTGCAA GCCTCATTTC 
ATTCACTGTA AGACCAACAG CCAGCAACGG AAGAGACGGG CAGAGTTGAA GCAACAAAAA 
GAGGAGGAGG GGATGTGGAA AGAGCAAGAA GCCGCTAGGC GAGACCCTCC CTCGGAAAGT 
TCTTGCGCTG CCGCTGCCAT CGCCACGCCG GAAGGCAGCC CCCCAGATGA GCCGACCTCC 
CCCAAGAAGC CCAAGAGCAT CCCGGAGCCT GAGCCTGGGG ATGTAGCAGG TGGAGCCGCC 
TCCCCCCTGC CCTCTGAGTG GACCGCAGTG AGAATCAGCC CCGGGGAGGA TGTGGTTGGA 
CAGGATGTGC TGGCCGTACG CGTCCTGGTC ACCAGCGATG ACAGCAGCTC CGATGCAGAA 
TTGGACCGTG GTGACAGTGA GGCCTCCAGT GTGGAGCCCT TTGATGAAAG ACCCCAGCAA 
CCGGAACTCC AGCTCCCACC CCTCCTGAAG CCCTTCACCC GGCACAGCTC CCTGAGGGAG 
GCCCTGCCCA GGGCAGTCTC TCCACATGAC CTCGGGGAGC CCGAAGCTGA GCCTGCTCTG 
CAGCGGGCCA ATAGTTTCCA GTGTCCAACT CCCAGCGAAT ACCAGAGTGG GAGAAGATTC 
CAGTCCAATT TCATGCCGGT GAACAGCAAA ATGACCGTGT CGCCTCCAAA GCAAGCTTGT 
CCTCCCCCGC CTTCTCCTTC TCCACCCTCT TCTTCCTCGA TTGGTGACCT GAATGCCTTG 
GACAGTCCCT CCCTGCCTCA GGTGACAGTT TCCAACACCG CCTCACAAAT CTCCAGAGGT 
CACTGCAGTC CATCCACCCC CATCTTTCTG AGGCGTGCCA AAGCCCATGG ACCACCCAAG 
GATCTCCCCC TGCGTCTGCC TCGTGGGCAG GTACTGGAGC GGACAGAATA CTGCCTGGTG 
AGGCCTGGTG GAAATGGCCT CCAGAGCTCC AGGCCACCCA GCCCTACAGA GATGGCTTCT 
GATGGGTGTC AGGAGGCAGT GGCCCCTCCT AGGGACATCA GAAGCATCCA CAGAGGCCCA 
CACCCCGTTG AAGGGAAGGA CAGGTGCTTG CCAGACCACC CACTGTCTCC CTGGGCTGGA 
GAGGAGCCTG GAGAAGGGAG TACCAGACCC AGGAGGGTAG AGGATGGTGG GTTAGAGCTG 
GCAGAGGAGA AGTCAGGCTT AAAGAAGTTG GTTCTCACTC CGGAGCAGAA GACTTGGTTG 
TTGGATTGGA ACGACTCCAA CCTTGAGAAC TTGTACCTGG AAACCGGAGC ACGACTTTCC 
CAAAAGAGCA CTAGAAATGG TTCAGGAGGC CATGTGCTGA AACCAGTCCA CCCCTTGCTG 
CTTCCTCTGG CAGAGAAAGA GACCTTGCCA GCCCAGAGAC AGTCTCAGGA GAAGATGGGG 
GCCCCAGCTG AACGGGCTCC TGGGGAACGA AGCATGGCCC CACCCAAGTC TCCACTGCGG 
CTTATAGCCA ACGCCATCCG GAGATCCCTG GAGCCGCTCC TCCCCAACTC TGACAGTGGG 
AGGAAGGCCT GGGCCAAGCC AGAGTCAAAG ACTTTGCCCA CCAGCCCACC TCATGCTTAC 
ACTTGCTCAT TCAGCTTTCG GAAAAGCAGC TCCAGTAAAG ACAGCGATCA GCCATCCCCC 
AAGAGAGACG TGGCAAGCAA GGCCTCAGCC TTCTTCTCCC TGGGCTCCCC GACTGCAAGG 
ACTGCCCAGC CCTCAGCCCC GAGCCCTCCT GCCCCCGCCC TTTGCACTCA CAGTTTGCCC 
AGCCGGTCAT CCAAGGTGTT TCCTGCACTT GTGCCCCCAC CCTGTGGCAA GATGGAAGAT 
GTCCCCACAC TCCTGGAGAA AGTGAGTTTG CAGGAGACAG TCCCAGATGC TTCCAGGGTT 
CCCAAGAAAA GGACCTCACT CTTCTCCTCC TTCAGACTCA AAGACAAATC TTTCGAGAGT 
TCCCTCCAAG AATCTGGACC AAGAAAGGAC TCCCAAGACC TCTTCAGCAG CCCCAAGGGG 
AAGGTGCAGC CCATGGGCAA TGCCCAGCCC CCAGAGAAGC AGGGGCAGCC AATCAGTAGC 
ACCTGCCTGG GGCAGAGGGT CCATCCTGTT TCTCCAGAGA AAGATGCAAG TCCCACTCAC 
GTCCCCATCA GAGCGCAGGG GACAGGGACT GTCTCTTCCA CCTCTTCCTC CACCACCTCC 
TCTGCAGATG AGGAATTCCA TCCCCAGCCT TCCTCGCGGT CAAAGGAGAG GAAGGCACTT 
AGAAGAAGAA GGAAGCTGGA AAAAGCAACA AAGCAATTGG TTAAGCAAGA AGAATTGAAA 
AGACTTCATA AAGCTCAGGC CATCCAGAGG CAGTTGGAGG AAGTGGAGGA GCAGCAGAGG 
GCTTTCGAGA TCCAGGGCGT GAGGTGGGAG AAGGCGCTGA GAGGAGAAGC AGCAGATTCA 
GGCACACAGG ATGAAGCACA GCTTTTGCAG GAATGGCTTA AGCTGGTTCT GGAGAAGAAT 
AAATTAATGC GATATGAGTC AGAGCTCCTG ATCATGGCCC ACGAACTAGA ACTGGAAGAT 
CATCAAAGCA GACTAGAGCA GAAATTAAGA GAGAAAATGC TCCAGGAAGA GAGCCAGAAA 
GATGAGAATG ATCTGAATGA AGAGCAAGAA ATACTCACCG AGATGATGCA AGTGATTGAG 
CAAAGGGACA AATTGGTGGA TTCCTTAGAG GAACAACGCA TCAGAGAGAA GGCCGAAGAC 
CAGCACTTTG AAAGCTTCAT ATTCTCTAGG GGCTGTCAGC TGAGCAGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466503.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321989.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.

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CloneID	OBa52585
Clone ID Related Accession (Same CDS sequence)	XM_015466503.1 , XM_010812540.2
Accession Version	XM_015466503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGCTGACAGT AGGGAAAGTA TCCAGCGGAA TAGGGGCTGC AGCTGAAGTT 
CTGGTCAATC TGTACTTGAA TGATCACAGA CCTAAGACTC AGGCCACCTC TCCAGACCTG 
GAGTCCGTTC GGAAGACGTT TCCCCTGAAC GTGGGTGGCA GCGACACCTG TTACTTCTGC 
AAGAAGCGCG TGTACGTCAT GGAGCGGCTG AGCGCGGAGG GCCGCTTCTT CCACCGGGAG 
TGTTTCCGCT GCAGCGTCTG CGCCACCACC TTGCACCTGG CCACCTACGC CTTCGACGTT 
GACGAAGGCA AGTTTTTCTG CAAGCCTCAT TTCATTCACT GTAAGACCAA CAGCCAGCAA 
CGGAAGAGAC GGGCAGAGTT GAAGCAACAA AAAGAGGAGG AGGGGATGTG GAAAGAGCAA 
GAAGCCGCTA GGCGAGACCC TCCCTCGGAA AGTTCTTGCG CTGCCGCTGC CATCGCCACG 
CCGGAAGGCA GCCCCCCAGA TGAGCCGACC TCCCCCAAGA AGCCCAAGAG CATCCCGGAG 
CCTGAGCCTG GGGATGTAGC AGGTGGAGCC GCCTCCCCCC TGCCCTCTGA GTGGACCGCA 
GTGAGAATCA GCCCCGGGGA GGATGTGGTT GGACAGGATG TGCTGGCCGT ACGCGTCCTG 
GTCACCAGCG ATGACAGCAG CTCCGATGCA GAATTGGACC GTGGTGACAG TGAGGCCTCC 
AGTGTGGAGC CCTTTGATGA AAGACCCCAG CAACCGGAAC TCCAGCTCCC ACCCCTCCTG 
AAGCCCTTCA CCCGGCACAG CTCCCTGAGG GAGGCCCTGC CCAGGGCAGT CTCTCCACAT 
GACCTCGGGG AGCCCGAAGC TGAGCCTGCT CTGCAGCGGG CCAATAGTTT CCAGTGTCCA 
ACTCCCAGCG AATACCAGAG TGGGAGAAGA TTCCAGTCCA ATTTCATGCC GGTGAACAGC 
AAAATGACCG TGTCGCCTCC AAAGCAAGCT TGTCCTCCCC CGCCTTCTCC TTCTCCACCC 
TCTTCTTCCT CGATTGGTGA CCTGAATGCC TTGGACAGTC CCTCCCTGCC TCAGGTGACA 
GTTTCCAACA CCGCCTCACA AATCTCCAGA GGTCACTGCA GTCCATCCAC CCCCATCTTT 
CTGAGGCGTG CCAAAGCCCA TGGACCACCC AAGGATCTCC CCCTGCGTCT GCCTCGTGGG 
CAGGTACTGG AGCGGACAGA ATACTGCCTG GTGAGGCCTG GTGGAAATGG CCTCCAGAGC 
TCCAGGCCAC CCAGCCCTAC AGAGATGGCT TCTGATGGGT GTCAGGAGGC AGTGGCCCCT 
CCTAGGGACA TCAGAAGCAT CCACAGAGGC CCACACCCCG TTGAAGGGAA GGACAGGTGC 
TTGCCAGACC ACCCACTGTC TCCCTGGGCT GGAGAGGAGC CTGGAGAAGG GAGTACCAGA 
CCCAGGAGGG TAGAGGATGG TGGGTTAGAG CTGGCAGAGG AGAAGTCAGG CTTAAAGAAG 
TTGGTTCTCA CTCCGGAGCA GAAGACTTGG TTGTTGGATT GGAACGACTC CAACCTTGAG 
AACTTGTACC TGGAAACCGG AGCACGACTT TCCCAAAAGA GCACTAGAAA TGGTTCAGGA 
GGCCATGTGC TGAAACCAGT CCACCCCTTG CTGCTTCCTC TGGCAGAGAA AGAGACCTTG 
CCAGCCCAGA GACAGTCTCA GGAGAAGATG GGGGCCCCAG CTGAACGGGC TCCTGGGGAA 
CGAAGCATGG CCCCACCCAA GTCTCCACTG CGGCTTATAG CCAACGCCAT CCGGAGATCC 
CTGGAGCCGC TCCTCCCCAA CTCTGACAGT GGGAGGAAGG CCTGGGCCAA GCCAGAGTCA 
AAGACTTTGC CCACCAGCCC ACCTCATGCT TACACTTGCT CATTCAGCTT TCGGAAAAGC 
AGCTCCAGTA AAGACAGCGA TCAGCCATCC CCCAAGAGAG ACGTGGCAAG CAAGGCCTCA 
GCCTTCTTCT CCCTGGGCTC CCCGACTGCA AGGACTGCCC AGCCCTCAGC CCCGAGCCCT 
CCTGCCCCCG CCCTTTGCAC TCACAGTTTG CCCAGCCGGT CATCCAAGGT GTTTCCTGCA 
CTTGTGCCCC CACCCTGTGG CAAGATGGAA GATGTCCCCA CACTCCTGGA GAAAGTGAGT 
TTGCAGGAGA CAGTCCCAGA TGCTTCCAGG GTTCCCAAGA AAAGGACCTC ACTCTTCTCC 
TCCTTCAGAC TCAAAGACAA ATCTTTCGAG AGTTCCCTCC AAGAATCTGG ACCAAGAAAG 
GACTCCCAAG ACCTCTTCAG CAGCCCCAAG GGGAAGGTGC AGCCCATGGG CAATGCCCAG 
CCCCCAGAGA AGCAGGGGCA GCCAATCAGT AGCACCTGCC TGGGGCAGAG GGTCCATCCT 
GTTTCTCCAG AGAAAGATGC AAGTCCCACT CACGTCCCCA TCAGAGCGCA GGGGACAGGG 
ACTGTCTCTT CCACCTCTTC CTCCACCACC TCCTCTGCAG ATGAGGAATT CCATCCCCAG 
CCTTCCTCGC GGTCAAAGGA GAGGAAGGCA CTTAGAAGAA GAAGGAAGCT GGAAAAAGCA 
ACAAAGCAAT TGGTTAAGCA AGAAGAATTG AAAAGACTTC ATAAAGCTCA GGCCATCCAG 
AGGCAGTTGG AGGAAGTGGA GGAGCAGCAG AGGGCTTTCG AGATCCAGGG CGTGAGGTGG 
GAGAAGGCGC TGAGAGGAGA AGCAGCAGAT TCAGGCACAC AGGATGAAGC ACAGCTTTTG 
CAGGAATGGC TTAAGCTGGT TCTGGAGAAG AATAAATTAA TGCGATATGA GTCAGAGCTC 
CTGATCATGG CCCACGAACT AGAACTGGAA GATCATCAAA GCAGACTAGA GCAGAAATTA 
AGAGAGAAAA TGCTCCAGGA AGAGAGCCAG AAAGATGAGA ATGATCTGAA TGAAGAGCAA 
GAAATACTCA CCGAGATGAT GCAAGTGATT GAGCAAAGGG ACAAATTGGT GGATTCCTTA 
GAGGAACAAC GCATCAGAGA GAAGGCCGAA GACCAGCACT TTGAAAGCTT CATATTCTCT 
AGGGGCTGTC AGCTGAGCAG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321989.1
CDS	330..6056
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466503.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466503.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGCTGACAGT AGGGAAAGTA TCCAGCGGAA TAGGGGCTGC AGCTGAAGTT 
CTGGTCAATC TGTACTTGAA TGATCACAGA CCTAAGACTC AGGCCACCTC TCCAGACCTG 
GAGTCCGTTC GGAAGACGTT TCCCCTGAAC GTGGGTGGCA GCGACACCTG TTACTTCTGC 
AAGAAGCGCG TGTACGTCAT GGAGCGGCTG AGCGCGGAGG GCCGCTTCTT CCACCGGGAG 
TGTTTCCGCT GCAGCGTCTG CGCCACCACC TTGCACCTGG CCACCTACGC CTTCGACGTT 
GACGAAGGCA AGTTTTTCTG CAAGCCTCAT TTCATTCACT GTAAGACCAA CAGCCAGCAA 
CGGAAGAGAC GGGCAGAGTT GAAGCAACAA AAAGAGGAGG AGGGGATGTG GAAAGAGCAA 
GAAGCCGCTA GGCGAGACCC TCCCTCGGAA AGTTCTTGCG CTGCCGCTGC CATCGCCACG 
CCGGAAGGCA GCCCCCCAGA TGAGCCGACC TCCCCCAAGA AGCCCAAGAG CATCCCGGAG 
CCTGAGCCTG GGGATGTAGC AGGTGGAGCC GCCTCCCCCC TGCCCTCTGA GTGGACCGCA 
GTGAGAATCA GCCCCGGGGA GGATGTGGTT GGACAGGATG TGCTGGCCGT ACGCGTCCTG 
GTCACCAGCG ATGACAGCAG CTCCGATGCA GAATTGGACC GTGGTGACAG TGAGGCCTCC 
AGTGTGGAGC CCTTTGATGA AAGACCCCAG CAACCGGAAC TCCAGCTCCC ACCCCTCCTG 
AAGCCCTTCA CCCGGCACAG CTCCCTGAGG GAGGCCCTGC CCAGGGCAGT CTCTCCACAT 
GACCTCGGGG AGCCCGAAGC TGAGCCTGCT CTGCAGCGGG CCAATAGTTT CCAGTGTCCA 
ACTCCCAGCG AATACCAGAG TGGGAGAAGA TTCCAGTCCA ATTTCATGCC GGTGAACAGC 
AAAATGACCG TGTCGCCTCC AAAGCAAGCT TGTCCTCCCC CGCCTTCTCC TTCTCCACCC 
TCTTCTTCCT CGATTGGTGA CCTGAATGCC TTGGACAGTC CCTCCCTGCC TCAGGTGACA 
GTTTCCAACA CCGCCTCACA AATCTCCAGA GGTCACTGCA GTCCATCCAC CCCCATCTTT 
CTGAGGCGTG CCAAAGCCCA TGGACCACCC AAGGATCTCC CCCTGCGTCT GCCTCGTGGG 
CAGGTACTGG AGCGGACAGA ATACTGCCTG GTGAGGCCTG GTGGAAATGG CCTCCAGAGC 
TCCAGGCCAC CCAGCCCTAC AGAGATGGCT TCTGATGGGT GTCAGGAGGC AGTGGCCCCT 
CCTAGGGACA TCAGAAGCAT CCACAGAGGC CCACACCCCG TTGAAGGGAA GGACAGGTGC 
TTGCCAGACC ACCCACTGTC TCCCTGGGCT GGAGAGGAGC CTGGAGAAGG GAGTACCAGA 
CCCAGGAGGG TAGAGGATGG TGGGTTAGAG CTGGCAGAGG AGAAGTCAGG CTTAAAGAAG 
TTGGTTCTCA CTCCGGAGCA GAAGACTTGG TTGTTGGATT GGAACGACTC CAACCTTGAG 
AACTTGTACC TGGAAACCGG AGCACGACTT TCCCAAAAGA GCACTAGAAA TGGTTCAGGA 
GGCCATGTGC TGAAACCAGT CCACCCCTTG CTGCTTCCTC TGGCAGAGAA AGAGACCTTG 
CCAGCCCAGA GACAGTCTCA GGAGAAGATG GGGGCCCCAG CTGAACGGGC TCCTGGGGAA 
CGAAGCATGG CCCCACCCAA GTCTCCACTG CGGCTTATAG CCAACGCCAT CCGGAGATCC 
CTGGAGCCGC TCCTCCCCAA CTCTGACAGT GGGAGGAAGG CCTGGGCCAA GCCAGAGTCA 
AAGACTTTGC CCACCAGCCC ACCTCATGCT TACACTTGCT CATTCAGCTT TCGGAAAAGC 
AGCTCCAGTA AAGACAGCGA TCAGCCATCC CCCAAGAGAG ACGTGGCAAG CAAGGCCTCA 
GCCTTCTTCT CCCTGGGCTC CCCGACTGCA AGGACTGCCC AGCCCTCAGC CCCGAGCCCT 
CCTGCCCCCG CCCTTTGCAC TCACAGTTTG CCCAGCCGGT CATCCAAGGT GTTTCCTGCA 
CTTGTGCCCC CACCCTGTGG CAAGATGGAA GATGTCCCCA CACTCCTGGA GAAAGTGAGT 
TTGCAGGAGA CAGTCCCAGA TGCTTCCAGG GTTCCCAAGA AAAGGACCTC ACTCTTCTCC 
TCCTTCAGAC TCAAAGACAA ATCTTTCGAG AGTTCCCTCC AAGAATCTGG ACCAAGAAAG 
GACTCCCAAG ACCTCTTCAG CAGCCCCAAG GGGAAGGTGC AGCCCATGGG CAATGCCCAG 
CCCCCAGAGA AGCAGGGGCA GCCAATCAGT AGCACCTGCC TGGGGCAGAG GGTCCATCCT 
GTTTCTCCAG AGAAAGATGC AAGTCCCACT CACGTCCCCA TCAGAGCGCA GGGGACAGGG 
ACTGTCTCTT CCACCTCTTC CTCCACCACC TCCTCTGCAG ATGAGGAATT CCATCCCCAG 
CCTTCCTCGC GGTCAAAGGA GAGGAAGGCA CTTAGAAGAA GAAGGAAGCT GGAAAAAGCA 
ACAAAGCAAT TGGTTAAGCA AGAAGAATTG AAAAGACTTC ATAAAGCTCA GGCCATCCAG 
AGGCAGTTGG AGGAAGTGGA GGAGCAGCAG AGGGCTTTCG AGATCCAGGG CGTGAGGTGG 
GAGAAGGCGC TGAGAGGAGA AGCAGCAGAT TCAGGCACAC AGGATGAAGC ACAGCTTTTG 
CAGGAATGGC TTAAGCTGGT TCTGGAGAAG AATAAATTAA TGCGATATGA GTCAGAGCTC 
CTGATCATGG CCCACGAACT AGAACTGGAA GATCATCAAA GCAGACTAGA GCAGAAATTA 
AGAGAGAAAA TGCTCCAGGA AGAGAGCCAG AAAGATGAGA ATGATCTGAA TGAAGAGCAA 
GAAATACTCA CCGAGATGAT GCAAGTGATT GAGCAAAGGG ACAAATTGGT GGATTCCTTA 
GAGGAACAAC GCATCAGAGA GAAGGCCGAA GACCAGCACT TTGAAAGCTT CATATTCTCT 
AGGGGCTGTC AGCTGAGCAG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812540.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810842.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.

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CloneID	OBa52585
Clone ID Related Accession (Same CDS sequence)	XM_015466503.1 , XM_010812540.2
Accession Version	XM_010812540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGCTGACAGT AGGGAAAGTA TCCAGCGGAA TAGGGGCTGC AGCTGAAGTT 
CTGGTCAATC TGTACTTGAA TGATCACAGA CCTAAGACTC AGGCCACCTC TCCAGACCTG 
GAGTCCGTTC GGAAGACGTT TCCCCTGAAC GTGGGTGGCA GCGACACCTG TTACTTCTGC 
AAGAAGCGCG TGTACGTCAT GGAGCGGCTG AGCGCGGAGG GCCGCTTCTT CCACCGGGAG 
TGTTTCCGCT GCAGCGTCTG CGCCACCACC TTGCACCTGG CCACCTACGC CTTCGACGTT 
GACGAAGGCA AGTTTTTCTG CAAGCCTCAT TTCATTCACT GTAAGACCAA CAGCCAGCAA 
CGGAAGAGAC GGGCAGAGTT GAAGCAACAA AAAGAGGAGG AGGGGATGTG GAAAGAGCAA 
GAAGCCGCTA GGCGAGACCC TCCCTCGGAA AGTTCTTGCG CTGCCGCTGC CATCGCCACG 
CCGGAAGGCA GCCCCCCAGA TGAGCCGACC TCCCCCAAGA AGCCCAAGAG CATCCCGGAG 
CCTGAGCCTG GGGATGTAGC AGGTGGAGCC GCCTCCCCCC TGCCCTCTGA GTGGACCGCA 
GTGAGAATCA GCCCCGGGGA GGATGTGGTT GGACAGGATG TGCTGGCCGT ACGCGTCCTG 
GTCACCAGCG ATGACAGCAG CTCCGATGCA GAATTGGACC GTGGTGACAG TGAGGCCTCC 
AGTGTGGAGC CCTTTGATGA AAGACCCCAG CAACCGGAAC TCCAGCTCCC ACCCCTCCTG 
AAGCCCTTCA CCCGGCACAG CTCCCTGAGG GAGGCCCTGC CCAGGGCAGT CTCTCCACAT 
GACCTCGGGG AGCCCGAAGC TGAGCCTGCT CTGCAGCGGG CCAATAGTTT CCAGTGTCCA 
ACTCCCAGCG AATACCAGAG TGGGAGAAGA TTCCAGTCCA ATTTCATGCC GGTGAACAGC 
AAAATGACCG TGTCGCCTCC AAAGCAAGCT TGTCCTCCCC CGCCTTCTCC TTCTCCACCC 
TCTTCTTCCT CGATTGGTGA CCTGAATGCC TTGGACAGTC CCTCCCTGCC TCAGGTGACA 
GTTTCCAACA CCGCCTCACA AATCTCCAGA GGTCACTGCA GTCCATCCAC CCCCATCTTT 
CTGAGGCGTG CCAAAGCCCA TGGACCACCC AAGGATCTCC CCCTGCGTCT GCCTCGTGGG 
CAGGTACTGG AGCGGACAGA ATACTGCCTG GTGAGGCCTG GTGGAAATGG CCTCCAGAGC 
TCCAGGCCAC CCAGCCCTAC AGAGATGGCT TCTGATGGGT GTCAGGAGGC AGTGGCCCCT 
CCTAGGGACA TCAGAAGCAT CCACAGAGGC CCACACCCCG TTGAAGGGAA GGACAGGTGC 
TTGCCAGACC ACCCACTGTC TCCCTGGGCT GGAGAGGAGC CTGGAGAAGG GAGTACCAGA 
CCCAGGAGGG TAGAGGATGG TGGGTTAGAG CTGGCAGAGG AGAAGTCAGG CTTAAAGAAG 
TTGGTTCTCA CTCCGGAGCA GAAGACTTGG TTGTTGGATT GGAACGACTC CAACCTTGAG 
AACTTGTACC TGGAAACCGG AGCACGACTT TCCCAAAAGA GCACTAGAAA TGGTTCAGGA 
GGCCATGTGC TGAAACCAGT CCACCCCTTG CTGCTTCCTC TGGCAGAGAA AGAGACCTTG 
CCAGCCCAGA GACAGTCTCA GGAGAAGATG GGGGCCCCAG CTGAACGGGC TCCTGGGGAA 
CGAAGCATGG CCCCACCCAA GTCTCCACTG CGGCTTATAG CCAACGCCAT CCGGAGATCC 
CTGGAGCCGC TCCTCCCCAA CTCTGACAGT GGGAGGAAGG CCTGGGCCAA GCCAGAGTCA 
AAGACTTTGC CCACCAGCCC ACCTCATGCT TACACTTGCT CATTCAGCTT TCGGAAAAGC 
AGCTCCAGTA AAGACAGCGA TCAGCCATCC CCCAAGAGAG ACGTGGCAAG CAAGGCCTCA 
GCCTTCTTCT CCCTGGGCTC CCCGACTGCA AGGACTGCCC AGCCCTCAGC CCCGAGCCCT 
CCTGCCCCCG CCCTTTGCAC TCACAGTTTG CCCAGCCGGT CATCCAAGGT GTTTCCTGCA 
CTTGTGCCCC CACCCTGTGG CAAGATGGAA GATGTCCCCA CACTCCTGGA GAAAGTGAGT 
TTGCAGGAGA CAGTCCCAGA TGCTTCCAGG GTTCCCAAGA AAAGGACCTC ACTCTTCTCC 
TCCTTCAGAC TCAAAGACAA ATCTTTCGAG AGTTCCCTCC AAGAATCTGG ACCAAGAAAG 
GACTCCCAAG ACCTCTTCAG CAGCCCCAAG GGGAAGGTGC AGCCCATGGG CAATGCCCAG 
CCCCCAGAGA AGCAGGGGCA GCCAATCAGT AGCACCTGCC TGGGGCAGAG GGTCCATCCT 
GTTTCTCCAG AGAAAGATGC AAGTCCCACT CACGTCCCCA TCAGAGCGCA GGGGACAGGG 
ACTGTCTCTT CCACCTCTTC CTCCACCACC TCCTCTGCAG ATGAGGAATT CCATCCCCAG 
CCTTCCTCGC GGTCAAAGGA GAGGAAGGCA CTTAGAAGAA GAAGGAAGCT GGAAAAAGCA 
ACAAAGCAAT TGGTTAAGCA AGAAGAATTG AAAAGACTTC ATAAAGCTCA GGCCATCCAG 
AGGCAGTTGG AGGAAGTGGA GGAGCAGCAG AGGGCTTTCG AGATCCAGGG CGTGAGGTGG 
GAGAAGGCGC TGAGAGGAGA AGCAGCAGAT TCAGGCACAC AGGATGAAGC ACAGCTTTTG 
CAGGAATGGC TTAAGCTGGT TCTGGAGAAG AATAAATTAA TGCGATATGA GTCAGAGCTC 
CTGATCATGG CCCACGAACT AGAACTGGAA GATCATCAAA GCAGACTAGA GCAGAAATTA 
AGAGAGAAAA TGCTCCAGGA AGAGAGCCAG AAAGATGAGA ATGATCTGAA TGAAGAGCAA 
GAAATACTCA CCGAGATGAT GCAAGTGATT GAGCAAAGGG ACAAATTGGT GGATTCCTTA 
GAGGAACAAC GCATCAGAGA GAAGGCCGAA GACCAGCACT TTGAAAGCTT CATATTCTCT 
AGGGGCTGTC AGCTGAGCAG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810842.1
CDS	330..6056
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQEHRVSGIGKPILRSSSDPPVNSRCPKPEEPTPSPSPPLKRQFPSVVVTGHVLRELKQVSAGGECPARPWRARAKSDLQLGGPENLASLPPTCPGALALSGVLRRLQQVEEKVLQKRAQNLANREFHKKNIKEKAAHLASMFGHGDFPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812540.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812540.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GCACCGTGTC 
TCAGGGATAG GTAAGCCTAT ACTGCGCTCT TCCTCTGACC CTCCTGTTAA CTCTCGCTGT 
CCCAAGCCGG AGGAGCCCAC ACCCAGCCCA TCACCACCTC TGAAAAGGCA GTTCCCCTCC 
GTGGTTGTGA CAGGACACGT GCTCCGAGAG CTAAAGCAAG TGTCTGCTGG TGGCGAGTGT 
CCAGCCAGGC CCTGGAGAGC CCGAGCCAAG TCTGACCTGC AGTTGGGCGG GCCAGAAAAC 
CTCGCCAGCC TGCCTCCCAC CTGCCCGGGA GCGCTGGCTC TCTCTGGGGT GCTGCGGCGG 
CTGCAGCAAG TGGAGGAAAA GGTTCTCCAG AAAAGGGCCC AGAACTTGGC CAACAGGGAA 
TTTCACAAAA AGAACATTAA GGAGAAGGCG GCTCACCTTG CCTCCATGTT TGGACACGGG 
GATTTCCCAC AGCTGACAGT AGGGAAAGTA TCCAGCGGAA TAGGGGCTGC AGCTGAAGTT 
CTGGTCAATC TGTACTTGAA TGATCACAGA CCTAAGACTC AGGCCACCTC TCCAGACCTG 
GAGTCCGTTC GGAAGACGTT TCCCCTGAAC GTGGGTGGCA GCGACACCTG TTACTTCTGC 
AAGAAGCGCG TGTACGTCAT GGAGCGGCTG AGCGCGGAGG GCCGCTTCTT CCACCGGGAG 
TGTTTCCGCT GCAGCGTCTG CGCCACCACC TTGCACCTGG CCACCTACGC CTTCGACGTT 
GACGAAGGCA AGTTTTTCTG CAAGCCTCAT TTCATTCACT GTAAGACCAA CAGCCAGCAA 
CGGAAGAGAC GGGCAGAGTT GAAGCAACAA AAAGAGGAGG AGGGGATGTG GAAAGAGCAA 
GAAGCCGCTA GGCGAGACCC TCCCTCGGAA AGTTCTTGCG CTGCCGCTGC CATCGCCACG 
CCGGAAGGCA GCCCCCCAGA TGAGCCGACC TCCCCCAAGA AGCCCAAGAG CATCCCGGAG 
CCTGAGCCTG GGGATGTAGC AGGTGGAGCC GCCTCCCCCC TGCCCTCTGA GTGGACCGCA 
GTGAGAATCA GCCCCGGGGA GGATGTGGTT GGACAGGATG TGCTGGCCGT ACGCGTCCTG 
GTCACCAGCG ATGACAGCAG CTCCGATGCA GAATTGGACC GTGGTGACAG TGAGGCCTCC 
AGTGTGGAGC CCTTTGATGA AAGACCCCAG CAACCGGAAC TCCAGCTCCC ACCCCTCCTG 
AAGCCCTTCA CCCGGCACAG CTCCCTGAGG GAGGCCCTGC CCAGGGCAGT CTCTCCACAT 
GACCTCGGGG AGCCCGAAGC TGAGCCTGCT CTGCAGCGGG CCAATAGTTT CCAGTGTCCA 
ACTCCCAGCG AATACCAGAG TGGGAGAAGA TTCCAGTCCA ATTTCATGCC GGTGAACAGC 
AAAATGACCG TGTCGCCTCC AAAGCAAGCT TGTCCTCCCC CGCCTTCTCC TTCTCCACCC 
TCTTCTTCCT CGATTGGTGA CCTGAATGCC TTGGACAGTC CCTCCCTGCC TCAGGTGACA 
GTTTCCAACA CCGCCTCACA AATCTCCAGA GGTCACTGCA GTCCATCCAC CCCCATCTTT 
CTGAGGCGTG CCAAAGCCCA TGGACCACCC AAGGATCTCC CCCTGCGTCT GCCTCGTGGG 
CAGGTACTGG AGCGGACAGA ATACTGCCTG GTGAGGCCTG GTGGAAATGG CCTCCAGAGC 
TCCAGGCCAC CCAGCCCTAC AGAGATGGCT TCTGATGGGT GTCAGGAGGC AGTGGCCCCT 
CCTAGGGACA TCAGAAGCAT CCACAGAGGC CCACACCCCG TTGAAGGGAA GGACAGGTGC 
TTGCCAGACC ACCCACTGTC TCCCTGGGCT GGAGAGGAGC CTGGAGAAGG GAGTACCAGA 
CCCAGGAGGG TAGAGGATGG TGGGTTAGAG CTGGCAGAGG AGAAGTCAGG CTTAAAGAAG 
TTGGTTCTCA CTCCGGAGCA GAAGACTTGG TTGTTGGATT GGAACGACTC CAACCTTGAG 
AACTTGTACC TGGAAACCGG AGCACGACTT TCCCAAAAGA GCACTAGAAA TGGTTCAGGA 
GGCCATGTGC TGAAACCAGT CCACCCCTTG CTGCTTCCTC TGGCAGAGAA AGAGACCTTG 
CCAGCCCAGA GACAGTCTCA GGAGAAGATG GGGGCCCCAG CTGAACGGGC TCCTGGGGAA 
CGAAGCATGG CCCCACCCAA GTCTCCACTG CGGCTTATAG CCAACGCCAT CCGGAGATCC 
CTGGAGCCGC TCCTCCCCAA CTCTGACAGT GGGAGGAAGG CCTGGGCCAA GCCAGAGTCA 
AAGACTTTGC CCACCAGCCC ACCTCATGCT TACACTTGCT CATTCAGCTT TCGGAAAAGC 
AGCTCCAGTA AAGACAGCGA TCAGCCATCC CCCAAGAGAG ACGTGGCAAG CAAGGCCTCA 
GCCTTCTTCT CCCTGGGCTC CCCGACTGCA AGGACTGCCC AGCCCTCAGC CCCGAGCCCT 
CCTGCCCCCG CCCTTTGCAC TCACAGTTTG CCCAGCCGGT CATCCAAGGT GTTTCCTGCA 
CTTGTGCCCC CACCCTGTGG CAAGATGGAA GATGTCCCCA CACTCCTGGA GAAAGTGAGT 
TTGCAGGAGA CAGTCCCAGA TGCTTCCAGG GTTCCCAAGA AAAGGACCTC ACTCTTCTCC 
TCCTTCAGAC TCAAAGACAA ATCTTTCGAG AGTTCCCTCC AAGAATCTGG ACCAAGAAAG 
GACTCCCAAG ACCTCTTCAG CAGCCCCAAG GGGAAGGTGC AGCCCATGGG CAATGCCCAG 
CCCCCAGAGA AGCAGGGGCA GCCAATCAGT AGCACCTGCC TGGGGCAGAG GGTCCATCCT 
GTTTCTCCAG AGAAAGATGC AAGTCCCACT CACGTCCCCA TCAGAGCGCA GGGGACAGGG 
ACTGTCTCTT CCACCTCTTC CTCCACCACC TCCTCTGCAG ATGAGGAATT CCATCCCCAG 
CCTTCCTCGC GGTCAAAGGA GAGGAAGGCA CTTAGAAGAA GAAGGAAGCT GGAAAAAGCA 
ACAAAGCAAT TGGTTAAGCA AGAAGAATTG AAAAGACTTC ATAAAGCTCA GGCCATCCAG 
AGGCAGTTGG AGGAAGTGGA GGAGCAGCAG AGGGCTTTCG AGATCCAGGG CGTGAGGTGG 
GAGAAGGCGC TGAGAGGAGA AGCAGCAGAT TCAGGCACAC AGGATGAAGC ACAGCTTTTG 
CAGGAATGGC TTAAGCTGGT TCTGGAGAAG AATAAATTAA TGCGATATGA GTCAGAGCTC 
CTGATCATGG CCCACGAACT AGAACTGGAA GATCATCAAA GCAGACTAGA GCAGAAATTA 
AGAGAGAAAA TGCTCCAGGA AGAGAGCCAG AAAGATGAGA ATGATCTGAA TGAAGAGCAA 
GAAATACTCA CCGAGATGAT GCAAGTGATT GAGCAAAGGG ACAAATTGGT GGATTCCTTA 
GAGGAACAAC GCATCAGAGA GAAGGCCGAA GACCAGCACT TTGAAAGCTT CATATTCTCT 
AGGGGCTGTC AGCTGAGCAG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812541.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810843.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.

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CloneID	OBa52586
Clone ID Related Accession (Same CDS sequence)	XM_010812541.2 , XM_015466504.1
Accession Version	XM_010812541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGAT 
GAGCCGACCT CCCCCAAGAA GCCCAAGAGC ATCCCGGAGC CTGAGCCTGG GGATGTAGCA 
GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG TGGACCGCAG TGAGAATCAG CCCCGGGGAG 
GATGTGGTTG GACAGGATGT GCTGGCCGTA CGCGTCCTGG TCACCAGCGA TGACAGCAGC 
TCCGATGCAG AATTGGACCG TGGTGACAGT GAGGCCTCCA GTGTGGAGCC CTTTGATGAA 
AGACCCCAGC AACCGGAACT CCAGCTCCCA CCCCTCCTGA AGCCCTTCAC CCGGCACAGC 
TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC TCTCCACATG ACCTCGGGGA GCCCGAAGCT 
GAGCCTGCTC TGCAGCGGGC CAATAGTTTC CAGTGTCCAA CTCCCAGCGA ATACCAGAGT 
GGGAGAAGAT TCCAGTCCAA TTTCATGCCG GTGAACAGCA AAATGACCGT GTCGCCTCCA 
AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT TCTCCACCCT CTTCTTCCTC GATTGGTGAC 
CTGAATGCCT TGGACAGTCC CTCCCTGCCT CAGGTGACAG TTTCCAACAC CGCCTCACAA 
ATCTCCAGAG GTCACTGCAG TCCATCCACC CCCATCTTTC TGAGGCGTGC CAAAGCCCAT 
GGACCACCCA AGGATCTCCC CCTGCGTCTG CCTCGTGGGC AGGTACTGGA GCGGACAGAA 
TACTGCCTGG TGAGGCCTGG TGGAAATGGC CTCCAGAGCT CCAGGCCACC CAGCCCTACA 
GAGATGGCTT CTGATGGGTG TCAGGAGGCA GTGGCCCCTC CTAGGGACAT CAGAAGCATC 
CACAGAGGCC CACACCCCGT TGAAGGGAAG GACAGGTGCT TGCCAGACCA CCCACTGTCT 
CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG AGTACCAGAC CCAGGAGGGT AGAGGATGGT 
GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG 
AAGACTTGGT TGTTGGATTG GAACGACTCC AACCTTGAGA ACTTGTACCT GGAAACCGGA 
GCACGACTTT CCCAAAAGAG CACTAGAAAT GGTTCAGGAG GCCATGTGCT GAAACCAGTC 
CACCCCTTGC TGCTTCCTCT GGCAGAGAAA GAGACCTTGC CAGCCCAGAG ACAGTCTCAG 
GAGAAGATGG GGGCCCCAGC TGAACGGGCT CCTGGGGAAC GAAGCATGGC CCCACCCAAG 
TCTCCACTGC GGCTTATAGC CAACGCCATC CGGAGATCCC TGGAGCCGCT CCTCCCCAAC 
TCTGACAGTG GGAGGAAGGC CTGGGCCAAG CCAGAGTCAA AGACTTTGCC CACCAGCCCA 
CCTCATGCTT ACACTTGCTC ATTCAGCTTT CGGAAAAGCA GCTCCAGTAA AGACAGCGAT 
CAGCCATCCC CCAAGAGAGA CGTGGCAAGC AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC 
CCGACTGCAA GGACTGCCCA GCCCTCAGCC CCGAGCCCTC CTGCCCCCGC CCTTTGCACT 
CACAGTTTGC CCAGCCGGTC ATCCAAGGTG TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC 
AAGATGGAAG ATGTCCCCAC ACTCCTGGAG AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT 
GCTTCCAGGG TTCCCAAGAA AAGGACCTCA CTCTTCTCCT CCTTCAGACT CAAAGACAAA 
TCTTTCGAGA GTTCCCTCCA AGAATCTGGA CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC 
AGCCCCAAGG GGAAGGTGCA GCCCATGGGC AATGCCCAGC CCCCAGAGAA GCAGGGGCAG 
CCAATCAGTA GCACCTGCCT GGGGCAGAGG GTCCATCCTG TTTCTCCAGA GAAAGATGCA 
AGTCCCACTC ACGTCCCCAT CAGAGCGCAG GGGACAGGGA CTGTCTCTTC CACCTCTTCC 
TCCACCACCT CCTCTGCAGA TGAGGAATTC CATCCCCAGC CTTCCTCGCG GTCAAAGGAG 
AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG GAAAAAGCAA CAAAGCAATT GGTTAAGCAA 
GAAGAATTGA AAAGACTTCA TAAAGCTCAG GCCATCCAGA GGCAGTTGGA GGAAGTGGAG 
GAGCAGCAGA GGGCTTTCGA GATCCAGGGC GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA 
GCAGCAGATT CAGGCACACA GGATGAAGCA CAGCTTTTGC AGGAATGGCT TAAGCTGGTT 
CTGGAGAAGA ATAAATTAAT GCGATATGAG TCAGAGCTCC TGATCATGGC CCACGAACTA 
GAACTGGAAG ATCATCAAAG CAGACTAGAG CAGAAATTAA GAGAGAAAAT GCTCCAGGAA 
GAGAGCCAGA AAGATGAGAA TGATCTGAAT GAAGAGCAAG AAATACTCAC CGAGATGATG 
CAAGTGATTG AGCAAAGGGA CAAATTGGTG GATTCCTTAG AGGAACAACG CATCAGAGAG 
AAGGCCGAAG ACCAGCACTT TGAAAGCTTC ATATTCTCTA GGGGCTGTCA GCTGAGCAGG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810843.1
CDS	330..5675
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812541.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812541.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGAT 
GAGCCGACCT CCCCCAAGAA GCCCAAGAGC ATCCCGGAGC CTGAGCCTGG GGATGTAGCA 
GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG TGGACCGCAG TGAGAATCAG CCCCGGGGAG 
GATGTGGTTG GACAGGATGT GCTGGCCGTA CGCGTCCTGG TCACCAGCGA TGACAGCAGC 
TCCGATGCAG AATTGGACCG TGGTGACAGT GAGGCCTCCA GTGTGGAGCC CTTTGATGAA 
AGACCCCAGC AACCGGAACT CCAGCTCCCA CCCCTCCTGA AGCCCTTCAC CCGGCACAGC 
TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC TCTCCACATG ACCTCGGGGA GCCCGAAGCT 
GAGCCTGCTC TGCAGCGGGC CAATAGTTTC CAGTGTCCAA CTCCCAGCGA ATACCAGAGT 
GGGAGAAGAT TCCAGTCCAA TTTCATGCCG GTGAACAGCA AAATGACCGT GTCGCCTCCA 
AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT TCTCCACCCT CTTCTTCCTC GATTGGTGAC 
CTGAATGCCT TGGACAGTCC CTCCCTGCCT CAGGTGACAG TTTCCAACAC CGCCTCACAA 
ATCTCCAGAG GTCACTGCAG TCCATCCACC CCCATCTTTC TGAGGCGTGC CAAAGCCCAT 
GGACCACCCA AGGATCTCCC CCTGCGTCTG CCTCGTGGGC AGGTACTGGA GCGGACAGAA 
TACTGCCTGG TGAGGCCTGG TGGAAATGGC CTCCAGAGCT CCAGGCCACC CAGCCCTACA 
GAGATGGCTT CTGATGGGTG TCAGGAGGCA GTGGCCCCTC CTAGGGACAT CAGAAGCATC 
CACAGAGGCC CACACCCCGT TGAAGGGAAG GACAGGTGCT TGCCAGACCA CCCACTGTCT 
CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG AGTACCAGAC CCAGGAGGGT AGAGGATGGT 
GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG 
AAGACTTGGT TGTTGGATTG GAACGACTCC AACCTTGAGA ACTTGTACCT GGAAACCGGA 
GCACGACTTT CCCAAAAGAG CACTAGAAAT GGTTCAGGAG GCCATGTGCT GAAACCAGTC 
CACCCCTTGC TGCTTCCTCT GGCAGAGAAA GAGACCTTGC CAGCCCAGAG ACAGTCTCAG 
GAGAAGATGG GGGCCCCAGC TGAACGGGCT CCTGGGGAAC GAAGCATGGC CCCACCCAAG 
TCTCCACTGC GGCTTATAGC CAACGCCATC CGGAGATCCC TGGAGCCGCT CCTCCCCAAC 
TCTGACAGTG GGAGGAAGGC CTGGGCCAAG CCAGAGTCAA AGACTTTGCC CACCAGCCCA 
CCTCATGCTT ACACTTGCTC ATTCAGCTTT CGGAAAAGCA GCTCCAGTAA AGACAGCGAT 
CAGCCATCCC CCAAGAGAGA CGTGGCAAGC AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC 
CCGACTGCAA GGACTGCCCA GCCCTCAGCC CCGAGCCCTC CTGCCCCCGC CCTTTGCACT 
CACAGTTTGC CCAGCCGGTC ATCCAAGGTG TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC 
AAGATGGAAG ATGTCCCCAC ACTCCTGGAG AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT 
GCTTCCAGGG TTCCCAAGAA AAGGACCTCA CTCTTCTCCT CCTTCAGACT CAAAGACAAA 
TCTTTCGAGA GTTCCCTCCA AGAATCTGGA CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC 
AGCCCCAAGG GGAAGGTGCA GCCCATGGGC AATGCCCAGC CCCCAGAGAA GCAGGGGCAG 
CCAATCAGTA GCACCTGCCT GGGGCAGAGG GTCCATCCTG TTTCTCCAGA GAAAGATGCA 
AGTCCCACTC ACGTCCCCAT CAGAGCGCAG GGGACAGGGA CTGTCTCTTC CACCTCTTCC 
TCCACCACCT CCTCTGCAGA TGAGGAATTC CATCCCCAGC CTTCCTCGCG GTCAAAGGAG 
AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG GAAAAAGCAA CAAAGCAATT GGTTAAGCAA 
GAAGAATTGA AAAGACTTCA TAAAGCTCAG GCCATCCAGA GGCAGTTGGA GGAAGTGGAG 
GAGCAGCAGA GGGCTTTCGA GATCCAGGGC GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA 
GCAGCAGATT CAGGCACACA GGATGAAGCA CAGCTTTTGC AGGAATGGCT TAAGCTGGTT 
CTGGAGAAGA ATAAATTAAT GCGATATGAG TCAGAGCTCC TGATCATGGC CCACGAACTA 
GAACTGGAAG ATCATCAAAG CAGACTAGAG CAGAAATTAA GAGAGAAAAT GCTCCAGGAA 
GAGAGCCAGA AAGATGAGAA TGATCTGAAT GAAGAGCAAG AAATACTCAC CGAGATGATG 
CAAGTGATTG AGCAAAGGGA CAAATTGGTG GATTCCTTAG AGGAACAACG CATCAGAGAG 
AAGGCCGAAG ACCAGCACTT TGAAAGCTTC ATATTCTCTA GGGGCTGTCA GCTGAGCAGG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466504.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321990.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.

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CloneID	OBa52586
Clone ID Related Accession (Same CDS sequence)	XM_010812541.2 , XM_015466504.1
Accession Version	XM_015466504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGAT 
GAGCCGACCT CCCCCAAGAA GCCCAAGAGC ATCCCGGAGC CTGAGCCTGG GGATGTAGCA 
GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG TGGACCGCAG TGAGAATCAG CCCCGGGGAG 
GATGTGGTTG GACAGGATGT GCTGGCCGTA CGCGTCCTGG TCACCAGCGA TGACAGCAGC 
TCCGATGCAG AATTGGACCG TGGTGACAGT GAGGCCTCCA GTGTGGAGCC CTTTGATGAA 
AGACCCCAGC AACCGGAACT CCAGCTCCCA CCCCTCCTGA AGCCCTTCAC CCGGCACAGC 
TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC TCTCCACATG ACCTCGGGGA GCCCGAAGCT 
GAGCCTGCTC TGCAGCGGGC CAATAGTTTC CAGTGTCCAA CTCCCAGCGA ATACCAGAGT 
GGGAGAAGAT TCCAGTCCAA TTTCATGCCG GTGAACAGCA AAATGACCGT GTCGCCTCCA 
AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT TCTCCACCCT CTTCTTCCTC GATTGGTGAC 
CTGAATGCCT TGGACAGTCC CTCCCTGCCT CAGGTGACAG TTTCCAACAC CGCCTCACAA 
ATCTCCAGAG GTCACTGCAG TCCATCCACC CCCATCTTTC TGAGGCGTGC CAAAGCCCAT 
GGACCACCCA AGGATCTCCC CCTGCGTCTG CCTCGTGGGC AGGTACTGGA GCGGACAGAA 
TACTGCCTGG TGAGGCCTGG TGGAAATGGC CTCCAGAGCT CCAGGCCACC CAGCCCTACA 
GAGATGGCTT CTGATGGGTG TCAGGAGGCA GTGGCCCCTC CTAGGGACAT CAGAAGCATC 
CACAGAGGCC CACACCCCGT TGAAGGGAAG GACAGGTGCT TGCCAGACCA CCCACTGTCT 
CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG AGTACCAGAC CCAGGAGGGT AGAGGATGGT 
GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG 
AAGACTTGGT TGTTGGATTG GAACGACTCC AACCTTGAGA ACTTGTACCT GGAAACCGGA 
GCACGACTTT CCCAAAAGAG CACTAGAAAT GGTTCAGGAG GCCATGTGCT GAAACCAGTC 
CACCCCTTGC TGCTTCCTCT GGCAGAGAAA GAGACCTTGC CAGCCCAGAG ACAGTCTCAG 
GAGAAGATGG GGGCCCCAGC TGAACGGGCT CCTGGGGAAC GAAGCATGGC CCCACCCAAG 
TCTCCACTGC GGCTTATAGC CAACGCCATC CGGAGATCCC TGGAGCCGCT CCTCCCCAAC 
TCTGACAGTG GGAGGAAGGC CTGGGCCAAG CCAGAGTCAA AGACTTTGCC CACCAGCCCA 
CCTCATGCTT ACACTTGCTC ATTCAGCTTT CGGAAAAGCA GCTCCAGTAA AGACAGCGAT 
CAGCCATCCC CCAAGAGAGA CGTGGCAAGC AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC 
CCGACTGCAA GGACTGCCCA GCCCTCAGCC CCGAGCCCTC CTGCCCCCGC CCTTTGCACT 
CACAGTTTGC CCAGCCGGTC ATCCAAGGTG TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC 
AAGATGGAAG ATGTCCCCAC ACTCCTGGAG AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT 
GCTTCCAGGG TTCCCAAGAA AAGGACCTCA CTCTTCTCCT CCTTCAGACT CAAAGACAAA 
TCTTTCGAGA GTTCCCTCCA AGAATCTGGA CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC 
AGCCCCAAGG GGAAGGTGCA GCCCATGGGC AATGCCCAGC CCCCAGAGAA GCAGGGGCAG 
CCAATCAGTA GCACCTGCCT GGGGCAGAGG GTCCATCCTG TTTCTCCAGA GAAAGATGCA 
AGTCCCACTC ACGTCCCCAT CAGAGCGCAG GGGACAGGGA CTGTCTCTTC CACCTCTTCC 
TCCACCACCT CCTCTGCAGA TGAGGAATTC CATCCCCAGC CTTCCTCGCG GTCAAAGGAG 
AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG GAAAAAGCAA CAAAGCAATT GGTTAAGCAA 
GAAGAATTGA AAAGACTTCA TAAAGCTCAG GCCATCCAGA GGCAGTTGGA GGAAGTGGAG 
GAGCAGCAGA GGGCTTTCGA GATCCAGGGC GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA 
GCAGCAGATT CAGGCACACA GGATGAAGCA CAGCTTTTGC AGGAATGGCT TAAGCTGGTT 
CTGGAGAAGA ATAAATTAAT GCGATATGAG TCAGAGCTCC TGATCATGGC CCACGAACTA 
GAACTGGAAG ATCATCAAAG CAGACTAGAG CAGAAATTAA GAGAGAAAAT GCTCCAGGAA 
GAGAGCCAGA AAGATGAGAA TGATCTGAAT GAAGAGCAAG AAATACTCAC CGAGATGATG 
CAAGTGATTG AGCAAAGGGA CAAATTGGTG GATTCCTTAG AGGAACAACG CATCAGAGAG 
AAGGCCGAAG ACCAGCACTT TGAAAGCTTC ATATTCTCTA GGGGCTGTCA GCTGAGCAGG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321990.1
CDS	330..5675
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466504.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466504.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GCTGACAGTA 
GGGAAAGTAT CCAGCGGAAT AGGGGCTGCA GCTGAAGTTC TGGTCAATCT GTACTTGAAT 
GATCACAGAC CTAAGACTCA GGCCACCTCT CCAGACCTGG AGTCCGTTCG GAAGACGTTT 
CCCCTGAACG TGGGTGGCAG CGACACCTGT TACTTCTGCA AGAAGCGCGT GTACGTCATG 
GAGCGGCTGA GCGCGGAGGG CCGCTTCTTC CACCGGGAGT GTTTCCGCTG CAGCGTCTGC 
GCCACCACCT TGCACCTGGC CACCTACGCC TTCGACGTTG ACGAAGGCAA GTTTTTCTGC 
AAGCCTCATT TCATTCACTG TAAGACCAAC AGCCAGCAAC GGAAGAGACG GGCAGAGTTG 
AAGCAACAAA AAGAGGAGGA GGGGATGTGG AAAGAGCAAG AAGCCGCTAG GCGAGACCCT 
CCCTCGGAAA GTTCTTGCGC TGCCGCTGCC ATCGCCACGC CGGAAGGCAG CCCCCCAGAT 
GAGCCGACCT CCCCCAAGAA GCCCAAGAGC ATCCCGGAGC CTGAGCCTGG GGATGTAGCA 
GGTGGAGCCG CCTCCCCCCT GCCCTCTGAG TGGACCGCAG TGAGAATCAG CCCCGGGGAG 
GATGTGGTTG GACAGGATGT GCTGGCCGTA CGCGTCCTGG TCACCAGCGA TGACAGCAGC 
TCCGATGCAG AATTGGACCG TGGTGACAGT GAGGCCTCCA GTGTGGAGCC CTTTGATGAA 
AGACCCCAGC AACCGGAACT CCAGCTCCCA CCCCTCCTGA AGCCCTTCAC CCGGCACAGC 
TCCCTGAGGG AGGCCCTGCC CAGGGCAGTC TCTCCACATG ACCTCGGGGA GCCCGAAGCT 
GAGCCTGCTC TGCAGCGGGC CAATAGTTTC CAGTGTCCAA CTCCCAGCGA ATACCAGAGT 
GGGAGAAGAT TCCAGTCCAA TTTCATGCCG GTGAACAGCA AAATGACCGT GTCGCCTCCA 
AAGCAAGCTT GTCCTCCCCC GCCTTCTCCT TCTCCACCCT CTTCTTCCTC GATTGGTGAC 
CTGAATGCCT TGGACAGTCC CTCCCTGCCT CAGGTGACAG TTTCCAACAC CGCCTCACAA 
ATCTCCAGAG GTCACTGCAG TCCATCCACC CCCATCTTTC TGAGGCGTGC CAAAGCCCAT 
GGACCACCCA AGGATCTCCC CCTGCGTCTG CCTCGTGGGC AGGTACTGGA GCGGACAGAA 
TACTGCCTGG TGAGGCCTGG TGGAAATGGC CTCCAGAGCT CCAGGCCACC CAGCCCTACA 
GAGATGGCTT CTGATGGGTG TCAGGAGGCA GTGGCCCCTC CTAGGGACAT CAGAAGCATC 
CACAGAGGCC CACACCCCGT TGAAGGGAAG GACAGGTGCT TGCCAGACCA CCCACTGTCT 
CCCTGGGCTG GAGAGGAGCC TGGAGAAGGG AGTACCAGAC CCAGGAGGGT AGAGGATGGT 
GGGTTAGAGC TGGCAGAGGA GAAGTCAGGC TTAAAGAAGT TGGTTCTCAC TCCGGAGCAG 
AAGACTTGGT TGTTGGATTG GAACGACTCC AACCTTGAGA ACTTGTACCT GGAAACCGGA 
GCACGACTTT CCCAAAAGAG CACTAGAAAT GGTTCAGGAG GCCATGTGCT GAAACCAGTC 
CACCCCTTGC TGCTTCCTCT GGCAGAGAAA GAGACCTTGC CAGCCCAGAG ACAGTCTCAG 
GAGAAGATGG GGGCCCCAGC TGAACGGGCT CCTGGGGAAC GAAGCATGGC CCCACCCAAG 
TCTCCACTGC GGCTTATAGC CAACGCCATC CGGAGATCCC TGGAGCCGCT CCTCCCCAAC 
TCTGACAGTG GGAGGAAGGC CTGGGCCAAG CCAGAGTCAA AGACTTTGCC CACCAGCCCA 
CCTCATGCTT ACACTTGCTC ATTCAGCTTT CGGAAAAGCA GCTCCAGTAA AGACAGCGAT 
CAGCCATCCC CCAAGAGAGA CGTGGCAAGC AAGGCCTCAG CCTTCTTCTC CCTGGGCTCC 
CCGACTGCAA GGACTGCCCA GCCCTCAGCC CCGAGCCCTC CTGCCCCCGC CCTTTGCACT 
CACAGTTTGC CCAGCCGGTC ATCCAAGGTG TTTCCTGCAC TTGTGCCCCC ACCCTGTGGC 
AAGATGGAAG ATGTCCCCAC ACTCCTGGAG AAAGTGAGTT TGCAGGAGAC AGTCCCAGAT 
GCTTCCAGGG TTCCCAAGAA AAGGACCTCA CTCTTCTCCT CCTTCAGACT CAAAGACAAA 
TCTTTCGAGA GTTCCCTCCA AGAATCTGGA CCAAGAAAGG ACTCCCAAGA CCTCTTCAGC 
AGCCCCAAGG GGAAGGTGCA GCCCATGGGC AATGCCCAGC CCCCAGAGAA GCAGGGGCAG 
CCAATCAGTA GCACCTGCCT GGGGCAGAGG GTCCATCCTG TTTCTCCAGA GAAAGATGCA 
AGTCCCACTC ACGTCCCCAT CAGAGCGCAG GGGACAGGGA CTGTCTCTTC CACCTCTTCC 
TCCACCACCT CCTCTGCAGA TGAGGAATTC CATCCCCAGC CTTCCTCGCG GTCAAAGGAG 
AGGAAGGCAC TTAGAAGAAG AAGGAAGCTG GAAAAAGCAA CAAAGCAATT GGTTAAGCAA 
GAAGAATTGA AAAGACTTCA TAAAGCTCAG GCCATCCAGA GGCAGTTGGA GGAAGTGGAG 
GAGCAGCAGA GGGCTTTCGA GATCCAGGGC GTGAGGTGGG AGAAGGCGCT GAGAGGAGAA 
GCAGCAGATT CAGGCACACA GGATGAAGCA CAGCTTTTGC AGGAATGGCT TAAGCTGGTT 
CTGGAGAAGA ATAAATTAAT GCGATATGAG TCAGAGCTCC TGATCATGGC CCACGAACTA 
GAACTGGAAG ATCATCAAAG CAGACTAGAG CAGAAATTAA GAGAGAAAAT GCTCCAGGAA 
GAGAGCCAGA AAGATGAGAA TGATCTGAAT GAAGAGCAAG AAATACTCAC CGAGATGATG 
CAAGTGATTG AGCAAAGGGA CAAATTGGTG GATTCCTTAG AGGAACAACG CATCAGAGAG 
AAGGCCGAAG ACCAGCACTT TGAAAGCTTC ATATTCTCTA GGGGCTGTCA GCTGAGCAGG 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812542.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810844.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.

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CloneID	OBa52587
Clone ID Related Accession (Same CDS sequence)	XM_010812542.2 , XM_015466505.1
Accession Version	XM_010812542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGCT GACAGTAGGG 
AAAGTATCCA GCGGAATAGG GGCTGCAGCT GAAGTTCTGG TCAATCTGTA CTTGAATGAT 
CACAGACCTA AGACTCAGGC CACCTCTCCA GACCTGGAGT CCGTTCGGAA GACGTTTCCC 
CTGAACGTGG GTGGCAGCGA CACCTGTTAC TTCTGCAAGA AGCGCGTGTA CGTCATGGAG 
CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC 
ACCACCTTGC ACCTGGCCAC CTACGCCTTC GACGTTGACG AAGGCAAGTT TTTCTGCAAG 
CCTCATTTCA TTCACTGTAA GACCAACAGC CAGCAACGGA AGAGACGGGC AGAGTTGAAG 
CAACAAAAAG AGGAGGAGGG GATGTGGAAA GAGCAAGAAG CCGCTAGGCG AGACCCTCCC 
TCGGAAAGTT CTTGCGCTGC CGCTGCCATC GCCACGCCGG AAGGCAGCCC CCCAGATGAG 
CCGACCTCCC CCAAGAAGCC CAAGAGCATC CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT 
GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG ACCGCAGTGA GAATCAGCCC CGGGGAGGAT 
GTGGTTGGAC AGGATGTGCT GGCCGTACGC GTCCTGGTCA CCAGCGATGA CAGCAGCTCC 
GATGCAGAAT TGGACCGTGG TGACAGTGAG GCCTCCAGTG TGGAGCCCTT TGATGAAAGA 
CCCCAGCAAC CGGAACTCCA GCTCCCACCC CTCCTGAAGC CCTTCACCCG GCACAGCTCC 
CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT CCACATGACC TCGGGGAGCC CGAAGCTGAG 
CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG TGTCCAACTC CCAGCGAATA CCAGAGTGGG 
AGAAGATTCC AGTCCAATTT CATGCCGGTG AACAGCAAAA TGACCGTGTC GCCTCCAAAG 
CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT CCACCCTCTT CTTCCTCGAT TGGTGACCTG 
AATGCCTTGG ACAGTCCCTC CCTGCCTCAG GTGACAGTTT CCAACACCGC CTCACAAATC 
TCCAGAGGTC ACTGCAGTCC ATCCACCCCC ATCTTTCTGA GGCGTGCCAA AGCCCATGGA 
CCACCCAAGG ATCTCCCCCT GCGTCTGCCT CGTGGGCAGG TACTGGAGCG GACAGAATAC 
TGCCTGGTGA GGCCTGGTGG AAATGGCCTC CAGAGCTCCA GGCCACCCAG CCCTACAGAG 
ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG GCCCCTCCTA GGGACATCAG AAGCATCCAC 
AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC AGGTGCTTGC CAGACCACCC ACTGTCTCCC 
TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT ACCAGACCCA GGAGGGTAGA GGATGGTGGG 
TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA AAGAAGTTGG TTCTCACTCC GGAGCAGAAG 
ACTTGGTTGT TGGATTGGAA CGACTCCAAC CTTGAGAACT TGTACCTGGA AACCGGAGCA 
CGACTTTCCC AAAAGAGCAC TAGAAATGGT TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC 
CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG ACCTTGCCAG CCCAGAGACA GTCTCAGGAG 
AAGATGGGGG CCCCAGCTGA ACGGGCTCCT GGGGAACGAA GCATGGCCCC ACCCAAGTCT 
CCACTGCGGC TTATAGCCAA CGCCATCCGG AGATCCCTGG AGCCGCTCCT CCCCAACTCT 
GACAGTGGGA GGAAGGCCTG GGCCAAGCCA GAGTCAAAGA CTTTGCCCAC CAGCCCACCT 
CATGCTTACA CTTGCTCATT CAGCTTTCGG AAAAGCAGCT CCAGTAAAGA CAGCGATCAG 
CCATCCCCCA AGAGAGACGT GGCAAGCAAG GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG 
ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG AGCCCTCCTG CCCCCGCCCT TTGCACTCAC 
AGTTTGCCCA GCCGGTCATC CAAGGTGTTT CCTGCACTTG TGCCCCCACC CTGTGGCAAG 
ATGGAAGATG TCCCCACACT CCTGGAGAAA GTGAGTTTGC AGGAGACAGT CCCAGATGCT 
TCCAGGGTTC CCAAGAAAAG GACCTCACTC TTCTCCTCCT TCAGACTCAA AGACAAATCT 
TTCGAGAGTT CCCTCCAAGA ATCTGGACCA AGAAAGGACT CCCAAGACCT CTTCAGCAGC 
CCCAAGGGGA AGGTGCAGCC CATGGGCAAT GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA 
ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC CATCCTGTTT CTCCAGAGAA AGATGCAAGT 
CCCACTCACG TCCCCATCAG AGCGCAGGGG ACAGGGACTG TCTCTTCCAC CTCTTCCTCC 
ACCACCTCCT CTGCAGATGA GGAATTCCAT CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG 
AAGGCACTTA GAAGAAGAAG GAAGCTGGAA AAAGCAACAA AGCAATTGGT TAAGCAAGAA 
GAATTGAAAA GACTTCATAA AGCTCAGGCC ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG 
CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA 
GCAGATTCAG GCACACAGGA TGAAGCACAG CTTTTGCAGG AATGGCTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCA GAGCTCCTGA TCATGGCCCA CGAACTAGAA 
CTGGAAGATC ATCAAAGCAG ACTAGAGCAG AAATTAAGAG AGAAAATGCT CCAGGAAGAG 
AGCCAGAAAG ATGAGAATGA TCTGAATGAA GAGCAAGAAA TACTCACCGA GATGATGCAA 
GTGATTGAGC AAAGGGACAA ATTGGTGGAT TCCTTAGAGG AACAACGCAT CAGAGAGAAG 
GCCGAAGACC AGCACTTTGA AAGCTTCATA TTCTCTAGGG GCTGTCAGCT GAGCAGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810844.1
CDS	330..5612
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812542.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812542.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGCT GACAGTAGGG 
AAAGTATCCA GCGGAATAGG GGCTGCAGCT GAAGTTCTGG TCAATCTGTA CTTGAATGAT 
CACAGACCTA AGACTCAGGC CACCTCTCCA GACCTGGAGT CCGTTCGGAA GACGTTTCCC 
CTGAACGTGG GTGGCAGCGA CACCTGTTAC TTCTGCAAGA AGCGCGTGTA CGTCATGGAG 
CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC 
ACCACCTTGC ACCTGGCCAC CTACGCCTTC GACGTTGACG AAGGCAAGTT TTTCTGCAAG 
CCTCATTTCA TTCACTGTAA GACCAACAGC CAGCAACGGA AGAGACGGGC AGAGTTGAAG 
CAACAAAAAG AGGAGGAGGG GATGTGGAAA GAGCAAGAAG CCGCTAGGCG AGACCCTCCC 
TCGGAAAGTT CTTGCGCTGC CGCTGCCATC GCCACGCCGG AAGGCAGCCC CCCAGATGAG 
CCGACCTCCC CCAAGAAGCC CAAGAGCATC CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT 
GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG ACCGCAGTGA GAATCAGCCC CGGGGAGGAT 
GTGGTTGGAC AGGATGTGCT GGCCGTACGC GTCCTGGTCA CCAGCGATGA CAGCAGCTCC 
GATGCAGAAT TGGACCGTGG TGACAGTGAG GCCTCCAGTG TGGAGCCCTT TGATGAAAGA 
CCCCAGCAAC CGGAACTCCA GCTCCCACCC CTCCTGAAGC CCTTCACCCG GCACAGCTCC 
CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT CCACATGACC TCGGGGAGCC CGAAGCTGAG 
CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG TGTCCAACTC CCAGCGAATA CCAGAGTGGG 
AGAAGATTCC AGTCCAATTT CATGCCGGTG AACAGCAAAA TGACCGTGTC GCCTCCAAAG 
CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT CCACCCTCTT CTTCCTCGAT TGGTGACCTG 
AATGCCTTGG ACAGTCCCTC CCTGCCTCAG GTGACAGTTT CCAACACCGC CTCACAAATC 
TCCAGAGGTC ACTGCAGTCC ATCCACCCCC ATCTTTCTGA GGCGTGCCAA AGCCCATGGA 
CCACCCAAGG ATCTCCCCCT GCGTCTGCCT CGTGGGCAGG TACTGGAGCG GACAGAATAC 
TGCCTGGTGA GGCCTGGTGG AAATGGCCTC CAGAGCTCCA GGCCACCCAG CCCTACAGAG 
ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG GCCCCTCCTA GGGACATCAG AAGCATCCAC 
AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC AGGTGCTTGC CAGACCACCC ACTGTCTCCC 
TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT ACCAGACCCA GGAGGGTAGA GGATGGTGGG 
TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA AAGAAGTTGG TTCTCACTCC GGAGCAGAAG 
ACTTGGTTGT TGGATTGGAA CGACTCCAAC CTTGAGAACT TGTACCTGGA AACCGGAGCA 
CGACTTTCCC AAAAGAGCAC TAGAAATGGT TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC 
CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG ACCTTGCCAG CCCAGAGACA GTCTCAGGAG 
AAGATGGGGG CCCCAGCTGA ACGGGCTCCT GGGGAACGAA GCATGGCCCC ACCCAAGTCT 
CCACTGCGGC TTATAGCCAA CGCCATCCGG AGATCCCTGG AGCCGCTCCT CCCCAACTCT 
GACAGTGGGA GGAAGGCCTG GGCCAAGCCA GAGTCAAAGA CTTTGCCCAC CAGCCCACCT 
CATGCTTACA CTTGCTCATT CAGCTTTCGG AAAAGCAGCT CCAGTAAAGA CAGCGATCAG 
CCATCCCCCA AGAGAGACGT GGCAAGCAAG GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG 
ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG AGCCCTCCTG CCCCCGCCCT TTGCACTCAC 
AGTTTGCCCA GCCGGTCATC CAAGGTGTTT CCTGCACTTG TGCCCCCACC CTGTGGCAAG 
ATGGAAGATG TCCCCACACT CCTGGAGAAA GTGAGTTTGC AGGAGACAGT CCCAGATGCT 
TCCAGGGTTC CCAAGAAAAG GACCTCACTC TTCTCCTCCT TCAGACTCAA AGACAAATCT 
TTCGAGAGTT CCCTCCAAGA ATCTGGACCA AGAAAGGACT CCCAAGACCT CTTCAGCAGC 
CCCAAGGGGA AGGTGCAGCC CATGGGCAAT GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA 
ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC CATCCTGTTT CTCCAGAGAA AGATGCAAGT 
CCCACTCACG TCCCCATCAG AGCGCAGGGG ACAGGGACTG TCTCTTCCAC CTCTTCCTCC 
ACCACCTCCT CTGCAGATGA GGAATTCCAT CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG 
AAGGCACTTA GAAGAAGAAG GAAGCTGGAA AAAGCAACAA AGCAATTGGT TAAGCAAGAA 
GAATTGAAAA GACTTCATAA AGCTCAGGCC ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG 
CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA 
GCAGATTCAG GCACACAGGA TGAAGCACAG CTTTTGCAGG AATGGCTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCA GAGCTCCTGA TCATGGCCCA CGAACTAGAA 
CTGGAAGATC ATCAAAGCAG ACTAGAGCAG AAATTAAGAG AGAAAATGCT CCAGGAAGAG 
AGCCAGAAAG ATGAGAATGA TCTGAATGAA GAGCAAGAAA TACTCACCGA GATGATGCAA 
GTGATTGAGC AAAGGGACAA ATTGGTGGAT TCCTTAGAGG AACAACGCAT CAGAGAGAAG 
GCCGAAGACC AGCACTTTGA AAGCTTCATA TTCTCTAGGG GCTGTCAGCT GAGCAGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466505.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321991.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.

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CloneID	OBa52587
Clone ID Related Accession (Same CDS sequence)	XM_010812542.2 , XM_015466505.1
Accession Version	XM_015466505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGCT GACAGTAGGG 
AAAGTATCCA GCGGAATAGG GGCTGCAGCT GAAGTTCTGG TCAATCTGTA CTTGAATGAT 
CACAGACCTA AGACTCAGGC CACCTCTCCA GACCTGGAGT CCGTTCGGAA GACGTTTCCC 
CTGAACGTGG GTGGCAGCGA CACCTGTTAC TTCTGCAAGA AGCGCGTGTA CGTCATGGAG 
CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC 
ACCACCTTGC ACCTGGCCAC CTACGCCTTC GACGTTGACG AAGGCAAGTT TTTCTGCAAG 
CCTCATTTCA TTCACTGTAA GACCAACAGC CAGCAACGGA AGAGACGGGC AGAGTTGAAG 
CAACAAAAAG AGGAGGAGGG GATGTGGAAA GAGCAAGAAG CCGCTAGGCG AGACCCTCCC 
TCGGAAAGTT CTTGCGCTGC CGCTGCCATC GCCACGCCGG AAGGCAGCCC CCCAGATGAG 
CCGACCTCCC CCAAGAAGCC CAAGAGCATC CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT 
GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG ACCGCAGTGA GAATCAGCCC CGGGGAGGAT 
GTGGTTGGAC AGGATGTGCT GGCCGTACGC GTCCTGGTCA CCAGCGATGA CAGCAGCTCC 
GATGCAGAAT TGGACCGTGG TGACAGTGAG GCCTCCAGTG TGGAGCCCTT TGATGAAAGA 
CCCCAGCAAC CGGAACTCCA GCTCCCACCC CTCCTGAAGC CCTTCACCCG GCACAGCTCC 
CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT CCACATGACC TCGGGGAGCC CGAAGCTGAG 
CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG TGTCCAACTC CCAGCGAATA CCAGAGTGGG 
AGAAGATTCC AGTCCAATTT CATGCCGGTG AACAGCAAAA TGACCGTGTC GCCTCCAAAG 
CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT CCACCCTCTT CTTCCTCGAT TGGTGACCTG 
AATGCCTTGG ACAGTCCCTC CCTGCCTCAG GTGACAGTTT CCAACACCGC CTCACAAATC 
TCCAGAGGTC ACTGCAGTCC ATCCACCCCC ATCTTTCTGA GGCGTGCCAA AGCCCATGGA 
CCACCCAAGG ATCTCCCCCT GCGTCTGCCT CGTGGGCAGG TACTGGAGCG GACAGAATAC 
TGCCTGGTGA GGCCTGGTGG AAATGGCCTC CAGAGCTCCA GGCCACCCAG CCCTACAGAG 
ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG GCCCCTCCTA GGGACATCAG AAGCATCCAC 
AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC AGGTGCTTGC CAGACCACCC ACTGTCTCCC 
TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT ACCAGACCCA GGAGGGTAGA GGATGGTGGG 
TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA AAGAAGTTGG TTCTCACTCC GGAGCAGAAG 
ACTTGGTTGT TGGATTGGAA CGACTCCAAC CTTGAGAACT TGTACCTGGA AACCGGAGCA 
CGACTTTCCC AAAAGAGCAC TAGAAATGGT TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC 
CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG ACCTTGCCAG CCCAGAGACA GTCTCAGGAG 
AAGATGGGGG CCCCAGCTGA ACGGGCTCCT GGGGAACGAA GCATGGCCCC ACCCAAGTCT 
CCACTGCGGC TTATAGCCAA CGCCATCCGG AGATCCCTGG AGCCGCTCCT CCCCAACTCT 
GACAGTGGGA GGAAGGCCTG GGCCAAGCCA GAGTCAAAGA CTTTGCCCAC CAGCCCACCT 
CATGCTTACA CTTGCTCATT CAGCTTTCGG AAAAGCAGCT CCAGTAAAGA CAGCGATCAG 
CCATCCCCCA AGAGAGACGT GGCAAGCAAG GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG 
ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG AGCCCTCCTG CCCCCGCCCT TTGCACTCAC 
AGTTTGCCCA GCCGGTCATC CAAGGTGTTT CCTGCACTTG TGCCCCCACC CTGTGGCAAG 
ATGGAAGATG TCCCCACACT CCTGGAGAAA GTGAGTTTGC AGGAGACAGT CCCAGATGCT 
TCCAGGGTTC CCAAGAAAAG GACCTCACTC TTCTCCTCCT TCAGACTCAA AGACAAATCT 
TTCGAGAGTT CCCTCCAAGA ATCTGGACCA AGAAAGGACT CCCAAGACCT CTTCAGCAGC 
CCCAAGGGGA AGGTGCAGCC CATGGGCAAT GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA 
ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC CATCCTGTTT CTCCAGAGAA AGATGCAAGT 
CCCACTCACG TCCCCATCAG AGCGCAGGGG ACAGGGACTG TCTCTTCCAC CTCTTCCTCC 
ACCACCTCCT CTGCAGATGA GGAATTCCAT CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG 
AAGGCACTTA GAAGAAGAAG GAAGCTGGAA AAAGCAACAA AGCAATTGGT TAAGCAAGAA 
GAATTGAAAA GACTTCATAA AGCTCAGGCC ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG 
CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA 
GCAGATTCAG GCACACAGGA TGAAGCACAG CTTTTGCAGG AATGGCTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCA GAGCTCCTGA TCATGGCCCA CGAACTAGAA 
CTGGAAGATC ATCAAAGCAG ACTAGAGCAG AAATTAAGAG AGAAAATGCT CCAGGAAGAG 
AGCCAGAAAG ATGAGAATGA TCTGAATGAA GAGCAAGAAA TACTCACCGA GATGATGCAA 
GTGATTGAGC AAAGGGACAA ATTGGTGGAT TCCTTAGAGG AACAACGCAT CAGAGAGAAG 
GCCGAAGACC AGCACTTTGA AAGCTTCATA TTCTCTAGGG GCTGTCAGCT GAGCAGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321991.1
CDS	330..5612
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQLTVGKVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466505.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466505.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGCT GACAGTAGGG 
AAAGTATCCA GCGGAATAGG GGCTGCAGCT GAAGTTCTGG TCAATCTGTA CTTGAATGAT 
CACAGACCTA AGACTCAGGC CACCTCTCCA GACCTGGAGT CCGTTCGGAA GACGTTTCCC 
CTGAACGTGG GTGGCAGCGA CACCTGTTAC TTCTGCAAGA AGCGCGTGTA CGTCATGGAG 
CGGCTGAGCG CGGAGGGCCG CTTCTTCCAC CGGGAGTGTT TCCGCTGCAG CGTCTGCGCC 
ACCACCTTGC ACCTGGCCAC CTACGCCTTC GACGTTGACG AAGGCAAGTT TTTCTGCAAG 
CCTCATTTCA TTCACTGTAA GACCAACAGC CAGCAACGGA AGAGACGGGC AGAGTTGAAG 
CAACAAAAAG AGGAGGAGGG GATGTGGAAA GAGCAAGAAG CCGCTAGGCG AGACCCTCCC 
TCGGAAAGTT CTTGCGCTGC CGCTGCCATC GCCACGCCGG AAGGCAGCCC CCCAGATGAG 
CCGACCTCCC CCAAGAAGCC CAAGAGCATC CCGGAGCCTG AGCCTGGGGA TGTAGCAGGT 
GGAGCCGCCT CCCCCCTGCC CTCTGAGTGG ACCGCAGTGA GAATCAGCCC CGGGGAGGAT 
GTGGTTGGAC AGGATGTGCT GGCCGTACGC GTCCTGGTCA CCAGCGATGA CAGCAGCTCC 
GATGCAGAAT TGGACCGTGG TGACAGTGAG GCCTCCAGTG TGGAGCCCTT TGATGAAAGA 
CCCCAGCAAC CGGAACTCCA GCTCCCACCC CTCCTGAAGC CCTTCACCCG GCACAGCTCC 
CTGAGGGAGG CCCTGCCCAG GGCAGTCTCT CCACATGACC TCGGGGAGCC CGAAGCTGAG 
CCTGCTCTGC AGCGGGCCAA TAGTTTCCAG TGTCCAACTC CCAGCGAATA CCAGAGTGGG 
AGAAGATTCC AGTCCAATTT CATGCCGGTG AACAGCAAAA TGACCGTGTC GCCTCCAAAG 
CAAGCTTGTC CTCCCCCGCC TTCTCCTTCT CCACCCTCTT CTTCCTCGAT TGGTGACCTG 
AATGCCTTGG ACAGTCCCTC CCTGCCTCAG GTGACAGTTT CCAACACCGC CTCACAAATC 
TCCAGAGGTC ACTGCAGTCC ATCCACCCCC ATCTTTCTGA GGCGTGCCAA AGCCCATGGA 
CCACCCAAGG ATCTCCCCCT GCGTCTGCCT CGTGGGCAGG TACTGGAGCG GACAGAATAC 
TGCCTGGTGA GGCCTGGTGG AAATGGCCTC CAGAGCTCCA GGCCACCCAG CCCTACAGAG 
ATGGCTTCTG ATGGGTGTCA GGAGGCAGTG GCCCCTCCTA GGGACATCAG AAGCATCCAC 
AGAGGCCCAC ACCCCGTTGA AGGGAAGGAC AGGTGCTTGC CAGACCACCC ACTGTCTCCC 
TGGGCTGGAG AGGAGCCTGG AGAAGGGAGT ACCAGACCCA GGAGGGTAGA GGATGGTGGG 
TTAGAGCTGG CAGAGGAGAA GTCAGGCTTA AAGAAGTTGG TTCTCACTCC GGAGCAGAAG 
ACTTGGTTGT TGGATTGGAA CGACTCCAAC CTTGAGAACT TGTACCTGGA AACCGGAGCA 
CGACTTTCCC AAAAGAGCAC TAGAAATGGT TCAGGAGGCC ATGTGCTGAA ACCAGTCCAC 
CCCTTGCTGC TTCCTCTGGC AGAGAAAGAG ACCTTGCCAG CCCAGAGACA GTCTCAGGAG 
AAGATGGGGG CCCCAGCTGA ACGGGCTCCT GGGGAACGAA GCATGGCCCC ACCCAAGTCT 
CCACTGCGGC TTATAGCCAA CGCCATCCGG AGATCCCTGG AGCCGCTCCT CCCCAACTCT 
GACAGTGGGA GGAAGGCCTG GGCCAAGCCA GAGTCAAAGA CTTTGCCCAC CAGCCCACCT 
CATGCTTACA CTTGCTCATT CAGCTTTCGG AAAAGCAGCT CCAGTAAAGA CAGCGATCAG 
CCATCCCCCA AGAGAGACGT GGCAAGCAAG GCCTCAGCCT TCTTCTCCCT GGGCTCCCCG 
ACTGCAAGGA CTGCCCAGCC CTCAGCCCCG AGCCCTCCTG CCCCCGCCCT TTGCACTCAC 
AGTTTGCCCA GCCGGTCATC CAAGGTGTTT CCTGCACTTG TGCCCCCACC CTGTGGCAAG 
ATGGAAGATG TCCCCACACT CCTGGAGAAA GTGAGTTTGC AGGAGACAGT CCCAGATGCT 
TCCAGGGTTC CCAAGAAAAG GACCTCACTC TTCTCCTCCT TCAGACTCAA AGACAAATCT 
TTCGAGAGTT CCCTCCAAGA ATCTGGACCA AGAAAGGACT CCCAAGACCT CTTCAGCAGC 
CCCAAGGGGA AGGTGCAGCC CATGGGCAAT GCCCAGCCCC CAGAGAAGCA GGGGCAGCCA 
ATCAGTAGCA CCTGCCTGGG GCAGAGGGTC CATCCTGTTT CTCCAGAGAA AGATGCAAGT 
CCCACTCACG TCCCCATCAG AGCGCAGGGG ACAGGGACTG TCTCTTCCAC CTCTTCCTCC 
ACCACCTCCT CTGCAGATGA GGAATTCCAT CCCCAGCCTT CCTCGCGGTC AAAGGAGAGG 
AAGGCACTTA GAAGAAGAAG GAAGCTGGAA AAAGCAACAA AGCAATTGGT TAAGCAAGAA 
GAATTGAAAA GACTTCATAA AGCTCAGGCC ATCCAGAGGC AGTTGGAGGA AGTGGAGGAG 
CAGCAGAGGG CTTTCGAGAT CCAGGGCGTG AGGTGGGAGA AGGCGCTGAG AGGAGAAGCA 
GCAGATTCAG GCACACAGGA TGAAGCACAG CTTTTGCAGG AATGGCTTAA GCTGGTTCTG 
GAGAAGAATA AATTAATGCG ATATGAGTCA GAGCTCCTGA TCATGGCCCA CGAACTAGAA 
CTGGAAGATC ATCAAAGCAG ACTAGAGCAG AAATTAAGAG AGAAAATGCT CCAGGAAGAG 
AGCCAGAAAG ATGAGAATGA TCTGAATGAA GAGCAAGAAA TACTCACCGA GATGATGCAA 
GTGATTGAGC AAAGGGACAA ATTGGTGGAT TCCTTAGAGG AACAACGCAT CAGAGAGAAG 
GCCGAAGACC AGCACTTTGA AAGCTTCATA TTCTCTAGGG GCTGTCAGCT GAGCAGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812543.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810845.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.

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CloneID	OBa52588
Clone ID Related Accession (Same CDS sequence)	XM_010812543.2 , XM_015466506.1
Accession Version	XM_010812543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG AAGCCCAAGA GCATCCCGGA GCCTGAGCCT 
GGGGATGTAG CAGGTGGAGC CGCCTCCCCC CTGCCCTCTG AGTGGACCGC AGTGAGAATC 
AGCCCCGGGG AGGATGTGGT TGGACAGGAT GTGCTGGCCG TACGCGTCCT GGTCACCAGC 
GATGACAGCA GCTCCGATGC AGAATTGGAC CGTGGTGACA GTGAGGCCTC CAGTGTGGAG 
CCCTTTGATG AAAGACCCCA GCAACCGGAA CTCCAGCTCC CACCCCTCCT GAAGCCCTTC 
ACCCGGCACA GCTCCCTGAG GGAGGCCCTG CCCAGGGCAG TCTCTCCACA TGACCTCGGG 
GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG GCCAATAGTT TCCAGTGTCC AACTCCCAGC 
GAATACCAGA GTGGGAGAAG ATTCCAGTCC AATTTCATGC CGGTGAACAG CAAAATGACC 
GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC CCGCCTTCTC CTTCTCCACC CTCTTCTTCC 
TCGATTGGTG ACCTGAATGC CTTGGACAGT CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC 
ACCGCCTCAC AAATCTCCAG AGGTCACTGC AGTCCATCCA CCCCCATCTT TCTGAGGCGT 
GCCAAAGCCC ATGGACCACC CAAGGATCTC CCCCTGCGTC TGCCTCGTGG GCAGGTACTG 
GAGCGGACAG AATACTGCCT GGTGAGGCCT GGTGGAAATG GCCTCCAGAG CTCCAGGCCA 
CCCAGCCCTA CAGAGATGGC TTCTGATGGG TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC 
ATCAGAAGCA TCCACAGAGG CCCACACCCC GTTGAAGGGA AGGACAGGTG CTTGCCAGAC 
CACCCACTGT CTCCCTGGGC TGGAGAGGAG CCTGGAGAAG GGAGTACCAG ACCCAGGAGG 
GTAGAGGATG GTGGGTTAGA GCTGGCAGAG GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC 
ACTCCGGAGC AGAAGACTTG GTTGTTGGAT TGGAACGACT CCAACCTTGA GAACTTGTAC 
CTGGAAACCG GAGCACGACT TTCCCAAAAG AGCACTAGAA ATGGTTCAGG AGGCCATGTG 
CTGAAACCAG TCCACCCCTT GCTGCTTCCT CTGGCAGAGA AAGAGACCTT GCCAGCCCAG 
AGACAGTCTC AGGAGAAGAT GGGGGCCCCA GCTGAACGGG CTCCTGGGGA ACGAAGCATG 
GCCCCACCCA AGTCTCCACT GCGGCTTATA GCCAACGCCA TCCGGAGATC CCTGGAGCCG 
CTCCTCCCCA ACTCTGACAG TGGGAGGAAG GCCTGGGCCA AGCCAGAGTC AAAGACTTTG 
CCCACCAGCC CACCTCATGC TTACACTTGC TCATTCAGCT TTCGGAAAAG CAGCTCCAGT 
AAAGACAGCG ATCAGCCATC CCCCAAGAGA GACGTGGCAA GCAAGGCCTC AGCCTTCTTC 
TCCCTGGGCT CCCCGACTGC AAGGACTGCC CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC 
GCCCTTTGCA CTCACAGTTT GCCCAGCCGG TCATCCAAGG TGTTTCCTGC ACTTGTGCCC 
CCACCCTGTG GCAAGATGGA AGATGTCCCC ACACTCCTGG AGAAAGTGAG TTTGCAGGAG 
ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG AAAAGGACCT CACTCTTCTC CTCCTTCAGA 
CTCAAAGACA AATCTTTCGA GAGTTCCCTC CAAGAATCTG GACCAAGAAA GGACTCCCAA 
GACCTCTTCA GCAGCCCCAA GGGGAAGGTG CAGCCCATGG GCAATGCCCA GCCCCCAGAG 
AAGCAGGGGC AGCCAATCAG TAGCACCTGC CTGGGGCAGA GGGTCCATCC TGTTTCTCCA 
GAGAAAGATG CAAGTCCCAC TCACGTCCCC ATCAGAGCGC AGGGGACAGG GACTGTCTCT 
TCCACCTCTT CCTCCACCAC CTCCTCTGCA GATGAGGAAT TCCATCCCCA GCCTTCCTCG 
CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA AGAAGGAAGC TGGAAAAAGC AACAAAGCAA 
TTGGTTAAGC AAGAAGAATT GAAAAGACTT CATAAAGCTC AGGCCATCCA GAGGCAGTTG 
GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC GAGATCCAGG GCGTGAGGTG GGAGAAGGCG 
CTGAGAGGAG AAGCAGCAGA TTCAGGCACA CAGGATGAAG CACAGCTTTT GCAGGAATGG 
CTTAAGCTGG TTCTGGAGAA GAATAAATTA ATGCGATATG AGTCAGAGCT CCTGATCATG 
GCCCACGAAC TAGAACTGGA AGATCATCAA AGCAGACTAG AGCAGAAATT AAGAGAGAAA 
ATGCTCCAGG AAGAGAGCCA GAAAGATGAG AATGATCTGA ATGAAGAGCA AGAAATACTC 
ACCGAGATGA TGCAAGTGAT TGAGCAAAGG GACAAATTGG TGGATTCCTT AGAGGAACAA 
CGCATCAGAG AGAAGGCCGA AGACCAGCAC TTTGAAAGCT TCATATTCTC TAGGGGCTGT 
CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810845.1
CDS	330..5567
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812543.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812543.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG AAGCCCAAGA GCATCCCGGA GCCTGAGCCT 
GGGGATGTAG CAGGTGGAGC CGCCTCCCCC CTGCCCTCTG AGTGGACCGC AGTGAGAATC 
AGCCCCGGGG AGGATGTGGT TGGACAGGAT GTGCTGGCCG TACGCGTCCT GGTCACCAGC 
GATGACAGCA GCTCCGATGC AGAATTGGAC CGTGGTGACA GTGAGGCCTC CAGTGTGGAG 
CCCTTTGATG AAAGACCCCA GCAACCGGAA CTCCAGCTCC CACCCCTCCT GAAGCCCTTC 
ACCCGGCACA GCTCCCTGAG GGAGGCCCTG CCCAGGGCAG TCTCTCCACA TGACCTCGGG 
GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG GCCAATAGTT TCCAGTGTCC AACTCCCAGC 
GAATACCAGA GTGGGAGAAG ATTCCAGTCC AATTTCATGC CGGTGAACAG CAAAATGACC 
GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC CCGCCTTCTC CTTCTCCACC CTCTTCTTCC 
TCGATTGGTG ACCTGAATGC CTTGGACAGT CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC 
ACCGCCTCAC AAATCTCCAG AGGTCACTGC AGTCCATCCA CCCCCATCTT TCTGAGGCGT 
GCCAAAGCCC ATGGACCACC CAAGGATCTC CCCCTGCGTC TGCCTCGTGG GCAGGTACTG 
GAGCGGACAG AATACTGCCT GGTGAGGCCT GGTGGAAATG GCCTCCAGAG CTCCAGGCCA 
CCCAGCCCTA CAGAGATGGC TTCTGATGGG TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC 
ATCAGAAGCA TCCACAGAGG CCCACACCCC GTTGAAGGGA AGGACAGGTG CTTGCCAGAC 
CACCCACTGT CTCCCTGGGC TGGAGAGGAG CCTGGAGAAG GGAGTACCAG ACCCAGGAGG 
GTAGAGGATG GTGGGTTAGA GCTGGCAGAG GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC 
ACTCCGGAGC AGAAGACTTG GTTGTTGGAT TGGAACGACT CCAACCTTGA GAACTTGTAC 
CTGGAAACCG GAGCACGACT TTCCCAAAAG AGCACTAGAA ATGGTTCAGG AGGCCATGTG 
CTGAAACCAG TCCACCCCTT GCTGCTTCCT CTGGCAGAGA AAGAGACCTT GCCAGCCCAG 
AGACAGTCTC AGGAGAAGAT GGGGGCCCCA GCTGAACGGG CTCCTGGGGA ACGAAGCATG 
GCCCCACCCA AGTCTCCACT GCGGCTTATA GCCAACGCCA TCCGGAGATC CCTGGAGCCG 
CTCCTCCCCA ACTCTGACAG TGGGAGGAAG GCCTGGGCCA AGCCAGAGTC AAAGACTTTG 
CCCACCAGCC CACCTCATGC TTACACTTGC TCATTCAGCT TTCGGAAAAG CAGCTCCAGT 
AAAGACAGCG ATCAGCCATC CCCCAAGAGA GACGTGGCAA GCAAGGCCTC AGCCTTCTTC 
TCCCTGGGCT CCCCGACTGC AAGGACTGCC CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC 
GCCCTTTGCA CTCACAGTTT GCCCAGCCGG TCATCCAAGG TGTTTCCTGC ACTTGTGCCC 
CCACCCTGTG GCAAGATGGA AGATGTCCCC ACACTCCTGG AGAAAGTGAG TTTGCAGGAG 
ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG AAAAGGACCT CACTCTTCTC CTCCTTCAGA 
CTCAAAGACA AATCTTTCGA GAGTTCCCTC CAAGAATCTG GACCAAGAAA GGACTCCCAA 
GACCTCTTCA GCAGCCCCAA GGGGAAGGTG CAGCCCATGG GCAATGCCCA GCCCCCAGAG 
AAGCAGGGGC AGCCAATCAG TAGCACCTGC CTGGGGCAGA GGGTCCATCC TGTTTCTCCA 
GAGAAAGATG CAAGTCCCAC TCACGTCCCC ATCAGAGCGC AGGGGACAGG GACTGTCTCT 
TCCACCTCTT CCTCCACCAC CTCCTCTGCA GATGAGGAAT TCCATCCCCA GCCTTCCTCG 
CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA AGAAGGAAGC TGGAAAAAGC AACAAAGCAA 
TTGGTTAAGC AAGAAGAATT GAAAAGACTT CATAAAGCTC AGGCCATCCA GAGGCAGTTG 
GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC GAGATCCAGG GCGTGAGGTG GGAGAAGGCG 
CTGAGAGGAG AAGCAGCAGA TTCAGGCACA CAGGATGAAG CACAGCTTTT GCAGGAATGG 
CTTAAGCTGG TTCTGGAGAA GAATAAATTA ATGCGATATG AGTCAGAGCT CCTGATCATG 
GCCCACGAAC TAGAACTGGA AGATCATCAA AGCAGACTAG AGCAGAAATT AAGAGAGAAA 
ATGCTCCAGG AAGAGAGCCA GAAAGATGAG AATGATCTGA ATGAAGAGCA AGAAATACTC 
ACCGAGATGA TGCAAGTGAT TGAGCAAAGG GACAAATTGG TGGATTCCTT AGAGGAACAA 
CGCATCAGAG AGAAGGCCGA AGACCAGCAC TTTGAAAGCT TCATATTCTC TAGGGGCTGT 
CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466506.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321992.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.

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CloneID	OBa52588
Clone ID Related Accession (Same CDS sequence)	XM_010812543.2 , XM_015466506.1
Accession Version	XM_015466506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG AAGCCCAAGA GCATCCCGGA GCCTGAGCCT 
GGGGATGTAG CAGGTGGAGC CGCCTCCCCC CTGCCCTCTG AGTGGACCGC AGTGAGAATC 
AGCCCCGGGG AGGATGTGGT TGGACAGGAT GTGCTGGCCG TACGCGTCCT GGTCACCAGC 
GATGACAGCA GCTCCGATGC AGAATTGGAC CGTGGTGACA GTGAGGCCTC CAGTGTGGAG 
CCCTTTGATG AAAGACCCCA GCAACCGGAA CTCCAGCTCC CACCCCTCCT GAAGCCCTTC 
ACCCGGCACA GCTCCCTGAG GGAGGCCCTG CCCAGGGCAG TCTCTCCACA TGACCTCGGG 
GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG GCCAATAGTT TCCAGTGTCC AACTCCCAGC 
GAATACCAGA GTGGGAGAAG ATTCCAGTCC AATTTCATGC CGGTGAACAG CAAAATGACC 
GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC CCGCCTTCTC CTTCTCCACC CTCTTCTTCC 
TCGATTGGTG ACCTGAATGC CTTGGACAGT CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC 
ACCGCCTCAC AAATCTCCAG AGGTCACTGC AGTCCATCCA CCCCCATCTT TCTGAGGCGT 
GCCAAAGCCC ATGGACCACC CAAGGATCTC CCCCTGCGTC TGCCTCGTGG GCAGGTACTG 
GAGCGGACAG AATACTGCCT GGTGAGGCCT GGTGGAAATG GCCTCCAGAG CTCCAGGCCA 
CCCAGCCCTA CAGAGATGGC TTCTGATGGG TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC 
ATCAGAAGCA TCCACAGAGG CCCACACCCC GTTGAAGGGA AGGACAGGTG CTTGCCAGAC 
CACCCACTGT CTCCCTGGGC TGGAGAGGAG CCTGGAGAAG GGAGTACCAG ACCCAGGAGG 
GTAGAGGATG GTGGGTTAGA GCTGGCAGAG GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC 
ACTCCGGAGC AGAAGACTTG GTTGTTGGAT TGGAACGACT CCAACCTTGA GAACTTGTAC 
CTGGAAACCG GAGCACGACT TTCCCAAAAG AGCACTAGAA ATGGTTCAGG AGGCCATGTG 
CTGAAACCAG TCCACCCCTT GCTGCTTCCT CTGGCAGAGA AAGAGACCTT GCCAGCCCAG 
AGACAGTCTC AGGAGAAGAT GGGGGCCCCA GCTGAACGGG CTCCTGGGGA ACGAAGCATG 
GCCCCACCCA AGTCTCCACT GCGGCTTATA GCCAACGCCA TCCGGAGATC CCTGGAGCCG 
CTCCTCCCCA ACTCTGACAG TGGGAGGAAG GCCTGGGCCA AGCCAGAGTC AAAGACTTTG 
CCCACCAGCC CACCTCATGC TTACACTTGC TCATTCAGCT TTCGGAAAAG CAGCTCCAGT 
AAAGACAGCG ATCAGCCATC CCCCAAGAGA GACGTGGCAA GCAAGGCCTC AGCCTTCTTC 
TCCCTGGGCT CCCCGACTGC AAGGACTGCC CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC 
GCCCTTTGCA CTCACAGTTT GCCCAGCCGG TCATCCAAGG TGTTTCCTGC ACTTGTGCCC 
CCACCCTGTG GCAAGATGGA AGATGTCCCC ACACTCCTGG AGAAAGTGAG TTTGCAGGAG 
ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG AAAAGGACCT CACTCTTCTC CTCCTTCAGA 
CTCAAAGACA AATCTTTCGA GAGTTCCCTC CAAGAATCTG GACCAAGAAA GGACTCCCAA 
GACCTCTTCA GCAGCCCCAA GGGGAAGGTG CAGCCCATGG GCAATGCCCA GCCCCCAGAG 
AAGCAGGGGC AGCCAATCAG TAGCACCTGC CTGGGGCAGA GGGTCCATCC TGTTTCTCCA 
GAGAAAGATG CAAGTCCCAC TCACGTCCCC ATCAGAGCGC AGGGGACAGG GACTGTCTCT 
TCCACCTCTT CCTCCACCAC CTCCTCTGCA GATGAGGAAT TCCATCCCCA GCCTTCCTCG 
CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA AGAAGGAAGC TGGAAAAAGC AACAAAGCAA 
TTGGTTAAGC AAGAAGAATT GAAAAGACTT CATAAAGCTC AGGCCATCCA GAGGCAGTTG 
GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC GAGATCCAGG GCGTGAGGTG GGAGAAGGCG 
CTGAGAGGAG AAGCAGCAGA TTCAGGCACA CAGGATGAAG CACAGCTTTT GCAGGAATGG 
CTTAAGCTGG TTCTGGAGAA GAATAAATTA ATGCGATATG AGTCAGAGCT CCTGATCATG 
GCCCACGAAC TAGAACTGGA AGATCATCAA AGCAGACTAG AGCAGAAATT AAGAGAGAAA 
ATGCTCCAGG AAGAGAGCCA GAAAGATGAG AATGATCTGA ATGAAGAGCA AGAAATACTC 
ACCGAGATGA TGCAAGTGAT TGAGCAAAGG GACAAATTGG TGGATTCCTT AGAGGAACAA 
CGCATCAGAG AGAAGGCCGA AGACCAGCAC TTTGAAAGCT TCATATTCTC TAGGGGCTGT 
CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321992.1
CDS	330..5567
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQAKKSPSGSHFQPSHLRTLRPQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466506.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466506.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGC AAAGAAGTCA 
CCTTCAGGTT CCCATTTCCA ACCCAGCCAC TTAAGAACAT TGCGGCCTCA GGAGTCCGTT 
CGGAAGACGT TTCCCCTGAA CGTGGGTGGC AGCGACACCT GTTACTTCTG CAAGAAGCGC 
GTGTACGTCA TGGAGCGGCT GAGCGCGGAG GGCCGCTTCT TCCACCGGGA GTGTTTCCGC 
TGCAGCGTCT GCGCCACCAC CTTGCACCTG GCCACCTACG CCTTCGACGT TGACGAAGGC 
AAGTTTTTCT GCAAGCCTCA TTTCATTCAC TGTAAGACCA ACAGCCAGCA ACGGAAGAGA 
CGGGCAGAGT TGAAGCAACA AAAAGAGGAG GAGGGGATGT GGAAAGAGCA AGAAGCCGCT 
AGGCGAGACC CTCCCTCGGA AAGTTCTTGC GCTGCCGCTG CCATCGCCAC GCCGGAAGGC 
AGCCCCCCAG ATGAGCCGAC CTCCCCCAAG AAGCCCAAGA GCATCCCGGA GCCTGAGCCT 
GGGGATGTAG CAGGTGGAGC CGCCTCCCCC CTGCCCTCTG AGTGGACCGC AGTGAGAATC 
AGCCCCGGGG AGGATGTGGT TGGACAGGAT GTGCTGGCCG TACGCGTCCT GGTCACCAGC 
GATGACAGCA GCTCCGATGC AGAATTGGAC CGTGGTGACA GTGAGGCCTC CAGTGTGGAG 
CCCTTTGATG AAAGACCCCA GCAACCGGAA CTCCAGCTCC CACCCCTCCT GAAGCCCTTC 
ACCCGGCACA GCTCCCTGAG GGAGGCCCTG CCCAGGGCAG TCTCTCCACA TGACCTCGGG 
GAGCCCGAAG CTGAGCCTGC TCTGCAGCGG GCCAATAGTT TCCAGTGTCC AACTCCCAGC 
GAATACCAGA GTGGGAGAAG ATTCCAGTCC AATTTCATGC CGGTGAACAG CAAAATGACC 
GTGTCGCCTC CAAAGCAAGC TTGTCCTCCC CCGCCTTCTC CTTCTCCACC CTCTTCTTCC 
TCGATTGGTG ACCTGAATGC CTTGGACAGT CCCTCCCTGC CTCAGGTGAC AGTTTCCAAC 
ACCGCCTCAC AAATCTCCAG AGGTCACTGC AGTCCATCCA CCCCCATCTT TCTGAGGCGT 
GCCAAAGCCC ATGGACCACC CAAGGATCTC CCCCTGCGTC TGCCTCGTGG GCAGGTACTG 
GAGCGGACAG AATACTGCCT GGTGAGGCCT GGTGGAAATG GCCTCCAGAG CTCCAGGCCA 
CCCAGCCCTA CAGAGATGGC TTCTGATGGG TGTCAGGAGG CAGTGGCCCC TCCTAGGGAC 
ATCAGAAGCA TCCACAGAGG CCCACACCCC GTTGAAGGGA AGGACAGGTG CTTGCCAGAC 
CACCCACTGT CTCCCTGGGC TGGAGAGGAG CCTGGAGAAG GGAGTACCAG ACCCAGGAGG 
GTAGAGGATG GTGGGTTAGA GCTGGCAGAG GAGAAGTCAG GCTTAAAGAA GTTGGTTCTC 
ACTCCGGAGC AGAAGACTTG GTTGTTGGAT TGGAACGACT CCAACCTTGA GAACTTGTAC 
CTGGAAACCG GAGCACGACT TTCCCAAAAG AGCACTAGAA ATGGTTCAGG AGGCCATGTG 
CTGAAACCAG TCCACCCCTT GCTGCTTCCT CTGGCAGAGA AAGAGACCTT GCCAGCCCAG 
AGACAGTCTC AGGAGAAGAT GGGGGCCCCA GCTGAACGGG CTCCTGGGGA ACGAAGCATG 
GCCCCACCCA AGTCTCCACT GCGGCTTATA GCCAACGCCA TCCGGAGATC CCTGGAGCCG 
CTCCTCCCCA ACTCTGACAG TGGGAGGAAG GCCTGGGCCA AGCCAGAGTC AAAGACTTTG 
CCCACCAGCC CACCTCATGC TTACACTTGC TCATTCAGCT TTCGGAAAAG CAGCTCCAGT 
AAAGACAGCG ATCAGCCATC CCCCAAGAGA GACGTGGCAA GCAAGGCCTC AGCCTTCTTC 
TCCCTGGGCT CCCCGACTGC AAGGACTGCC CAGCCCTCAG CCCCGAGCCC TCCTGCCCCC 
GCCCTTTGCA CTCACAGTTT GCCCAGCCGG TCATCCAAGG TGTTTCCTGC ACTTGTGCCC 
CCACCCTGTG GCAAGATGGA AGATGTCCCC ACACTCCTGG AGAAAGTGAG TTTGCAGGAG 
ACAGTCCCAG ATGCTTCCAG GGTTCCCAAG AAAAGGACCT CACTCTTCTC CTCCTTCAGA 
CTCAAAGACA AATCTTTCGA GAGTTCCCTC CAAGAATCTG GACCAAGAAA GGACTCCCAA 
GACCTCTTCA GCAGCCCCAA GGGGAAGGTG CAGCCCATGG GCAATGCCCA GCCCCCAGAG 
AAGCAGGGGC AGCCAATCAG TAGCACCTGC CTGGGGCAGA GGGTCCATCC TGTTTCTCCA 
GAGAAAGATG CAAGTCCCAC TCACGTCCCC ATCAGAGCGC AGGGGACAGG GACTGTCTCT 
TCCACCTCTT CCTCCACCAC CTCCTCTGCA GATGAGGAAT TCCATCCCCA GCCTTCCTCG 
CGGTCAAAGG AGAGGAAGGC ACTTAGAAGA AGAAGGAAGC TGGAAAAAGC AACAAAGCAA 
TTGGTTAAGC AAGAAGAATT GAAAAGACTT CATAAAGCTC AGGCCATCCA GAGGCAGTTG 
GAGGAAGTGG AGGAGCAGCA GAGGGCTTTC GAGATCCAGG GCGTGAGGTG GGAGAAGGCG 
CTGAGAGGAG AAGCAGCAGA TTCAGGCACA CAGGATGAAG CACAGCTTTT GCAGGAATGG 
CTTAAGCTGG TTCTGGAGAA GAATAAATTA ATGCGATATG AGTCAGAGCT CCTGATCATG 
GCCCACGAAC TAGAACTGGA AGATCATCAA AGCAGACTAG AGCAGAAATT AAGAGAGAAA 
ATGCTCCAGG AAGAGAGCCA GAAAGATGAG AATGATCTGA ATGAAGAGCA AGAAATACTC 
ACCGAGATGA TGCAAGTGAT TGAGCAAAGG GACAAATTGG TGGATTCCTT AGAGGAACAA 
CGCATCAGAG AGAAGGCCGA AGACCAGCAC TTTGAAAGCT TCATATTCTC TAGGGGCTGT 
CAGCTGAGCA GGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_015466507.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321993.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.

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CloneID	OBa52589
Clone ID Related Accession (Same CDS sequence)	XM_015466507.1 , XM_010812544.2
Accession Version	XM_015466507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGATG AGCCGACCTC CCCCAAGAAG CCCAAGAGCA TCCCGGAGCC TGAGCCTGGG 
GATGTAGCAG GTGGAGCCGC CTCCCCCCTG CCCTCTGAGT GGACCGCAGT GAGAATCAGC 
CCCGGGGAGG ATGTGGTTGG ACAGGATGTG CTGGCCGTAC GCGTCCTGGT CACCAGCGAT 
GACAGCAGCT CCGATGCAGA ATTGGACCGT GGTGACAGTG AGGCCTCCAG TGTGGAGCCC 
TTTGATGAAA GACCCCAGCA ACCGGAACTC CAGCTCCCAC CCCTCCTGAA GCCCTTCACC 
CGGCACAGCT CCCTGAGGGA GGCCCTGCCC AGGGCAGTCT CTCCACATGA CCTCGGGGAG 
CCCGAAGCTG AGCCTGCTCT GCAGCGGGCC AATAGTTTCC AGTGTCCAAC TCCCAGCGAA 
TACCAGAGTG GGAGAAGATT CCAGTCCAAT TTCATGCCGG TGAACAGCAA AATGACCGTG 
TCGCCTCCAA AGCAAGCTTG TCCTCCCCCG CCTTCTCCTT CTCCACCCTC TTCTTCCTCG 
ATTGGTGACC TGAATGCCTT GGACAGTCCC TCCCTGCCTC AGGTGACAGT TTCCAACACC 
GCCTCACAAA TCTCCAGAGG TCACTGCAGT CCATCCACCC CCATCTTTCT GAGGCGTGCC 
AAAGCCCATG GACCACCCAA GGATCTCCCC CTGCGTCTGC CTCGTGGGCA GGTACTGGAG 
CGGACAGAAT ACTGCCTGGT GAGGCCTGGT GGAAATGGCC TCCAGAGCTC CAGGCCACCC 
AGCCCTACAG AGATGGCTTC TGATGGGTGT CAGGAGGCAG TGGCCCCTCC TAGGGACATC 
AGAAGCATCC ACAGAGGCCC ACACCCCGTT GAAGGGAAGG ACAGGTGCTT GCCAGACCAC 
CCACTGTCTC CCTGGGCTGG AGAGGAGCCT GGAGAAGGGA GTACCAGACC CAGGAGGGTA 
GAGGATGGTG GGTTAGAGCT GGCAGAGGAG AAGTCAGGCT TAAAGAAGTT GGTTCTCACT 
CCGGAGCAGA AGACTTGGTT GTTGGATTGG AACGACTCCA ACCTTGAGAA CTTGTACCTG 
GAAACCGGAG CACGACTTTC CCAAAAGAGC ACTAGAAATG GTTCAGGAGG CCATGTGCTG 
AAACCAGTCC ACCCCTTGCT GCTTCCTCTG GCAGAGAAAG AGACCTTGCC AGCCCAGAGA 
CAGTCTCAGG AGAAGATGGG GGCCCCAGCT GAACGGGCTC CTGGGGAACG AAGCATGGCC 
CCACCCAAGT CTCCACTGCG GCTTATAGCC AACGCCATCC GGAGATCCCT GGAGCCGCTC 
CTCCCCAACT CTGACAGTGG GAGGAAGGCC TGGGCCAAGC CAGAGTCAAA GACTTTGCCC 
ACCAGCCCAC CTCATGCTTA CACTTGCTCA TTCAGCTTTC GGAAAAGCAG CTCCAGTAAA 
GACAGCGATC AGCCATCCCC CAAGAGAGAC GTGGCAAGCA AGGCCTCAGC CTTCTTCTCC 
CTGGGCTCCC CGACTGCAAG GACTGCCCAG CCCTCAGCCC CGAGCCCTCC TGCCCCCGCC 
CTTTGCACTC ACAGTTTGCC CAGCCGGTCA TCCAAGGTGT TTCCTGCACT TGTGCCCCCA 
CCCTGTGGCA AGATGGAAGA TGTCCCCACA CTCCTGGAGA AAGTGAGTTT GCAGGAGACA 
GTCCCAGATG CTTCCAGGGT TCCCAAGAAA AGGACCTCAC TCTTCTCCTC CTTCAGACTC 
AAAGACAAAT CTTTCGAGAG TTCCCTCCAA GAATCTGGAC CAAGAAAGGA CTCCCAAGAC 
CTCTTCAGCA GCCCCAAGGG GAAGGTGCAG CCCATGGGCA ATGCCCAGCC CCCAGAGAAG 
CAGGGGCAGC CAATCAGTAG CACCTGCCTG GGGCAGAGGG TCCATCCTGT TTCTCCAGAG 
AAAGATGCAA GTCCCACTCA CGTCCCCATC AGAGCGCAGG GGACAGGGAC TGTCTCTTCC 
ACCTCTTCCT CCACCACCTC CTCTGCAGAT GAGGAATTCC ATCCCCAGCC TTCCTCGCGG 
TCAAAGGAGA GGAAGGCACT TAGAAGAAGA AGGAAGCTGG AAAAAGCAAC AAAGCAATTG 
GTTAAGCAAG AAGAATTGAA AAGACTTCAT AAAGCTCAGG CCATCCAGAG GCAGTTGGAG 
GAAGTGGAGG AGCAGCAGAG GGCTTTCGAG ATCCAGGGCG TGAGGTGGGA GAAGGCGCTG 
AGAGGAGAAG CAGCAGATTC AGGCACACAG GATGAAGCAC AGCTTTTGCA GGAATGGCTT 
AAGCTGGTTC TGGAGAAGAA TAAATTAATG CGATATGAGT CAGAGCTCCT GATCATGGCC 
CACGAACTAG AACTGGAAGA TCATCAAAGC AGACTAGAGC AGAAATTAAG AGAGAAAATG 
CTCCAGGAAG AGAGCCAGAA AGATGAGAAT GATCTGAATG AAGAGCAAGA AATACTCACC 
GAGATGATGC AAGTGATTGA GCAAAGGGAC AAATTGGTGG ATTCCTTAGA GGAACAACGC 
ATCAGAGAGA AGGCCGAAGA CCAGCACTTT GAAAGCTTCA TATTCTCTAG GGGCTGTCAG 
CTGAGCAGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321993.1
CDS	330..5504
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_015466507.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466507.1

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGATG AGCCGACCTC CCCCAAGAAG CCCAAGAGCA TCCCGGAGCC TGAGCCTGGG 
GATGTAGCAG GTGGAGCCGC CTCCCCCCTG CCCTCTGAGT GGACCGCAGT GAGAATCAGC 
CCCGGGGAGG ATGTGGTTGG ACAGGATGTG CTGGCCGTAC GCGTCCTGGT CACCAGCGAT 
GACAGCAGCT CCGATGCAGA ATTGGACCGT GGTGACAGTG AGGCCTCCAG TGTGGAGCCC 
TTTGATGAAA GACCCCAGCA ACCGGAACTC CAGCTCCCAC CCCTCCTGAA GCCCTTCACC 
CGGCACAGCT CCCTGAGGGA GGCCCTGCCC AGGGCAGTCT CTCCACATGA CCTCGGGGAG 
CCCGAAGCTG AGCCTGCTCT GCAGCGGGCC AATAGTTTCC AGTGTCCAAC TCCCAGCGAA 
TACCAGAGTG GGAGAAGATT CCAGTCCAAT TTCATGCCGG TGAACAGCAA AATGACCGTG 
TCGCCTCCAA AGCAAGCTTG TCCTCCCCCG CCTTCTCCTT CTCCACCCTC TTCTTCCTCG 
ATTGGTGACC TGAATGCCTT GGACAGTCCC TCCCTGCCTC AGGTGACAGT TTCCAACACC 
GCCTCACAAA TCTCCAGAGG TCACTGCAGT CCATCCACCC CCATCTTTCT GAGGCGTGCC 
AAAGCCCATG GACCACCCAA GGATCTCCCC CTGCGTCTGC CTCGTGGGCA GGTACTGGAG 
CGGACAGAAT ACTGCCTGGT GAGGCCTGGT GGAAATGGCC TCCAGAGCTC CAGGCCACCC 
AGCCCTACAG AGATGGCTTC TGATGGGTGT CAGGAGGCAG TGGCCCCTCC TAGGGACATC 
AGAAGCATCC ACAGAGGCCC ACACCCCGTT GAAGGGAAGG ACAGGTGCTT GCCAGACCAC 
CCACTGTCTC CCTGGGCTGG AGAGGAGCCT GGAGAAGGGA GTACCAGACC CAGGAGGGTA 
GAGGATGGTG GGTTAGAGCT GGCAGAGGAG AAGTCAGGCT TAAAGAAGTT GGTTCTCACT 
CCGGAGCAGA AGACTTGGTT GTTGGATTGG AACGACTCCA ACCTTGAGAA CTTGTACCTG 
GAAACCGGAG CACGACTTTC CCAAAAGAGC ACTAGAAATG GTTCAGGAGG CCATGTGCTG 
AAACCAGTCC ACCCCTTGCT GCTTCCTCTG GCAGAGAAAG AGACCTTGCC AGCCCAGAGA 
CAGTCTCAGG AGAAGATGGG GGCCCCAGCT GAACGGGCTC CTGGGGAACG AAGCATGGCC 
CCACCCAAGT CTCCACTGCG GCTTATAGCC AACGCCATCC GGAGATCCCT GGAGCCGCTC 
CTCCCCAACT CTGACAGTGG GAGGAAGGCC TGGGCCAAGC CAGAGTCAAA GACTTTGCCC 
ACCAGCCCAC CTCATGCTTA CACTTGCTCA TTCAGCTTTC GGAAAAGCAG CTCCAGTAAA 
GACAGCGATC AGCCATCCCC CAAGAGAGAC GTGGCAAGCA AGGCCTCAGC CTTCTTCTCC 
CTGGGCTCCC CGACTGCAAG GACTGCCCAG CCCTCAGCCC CGAGCCCTCC TGCCCCCGCC 
CTTTGCACTC ACAGTTTGCC CAGCCGGTCA TCCAAGGTGT TTCCTGCACT TGTGCCCCCA 
CCCTGTGGCA AGATGGAAGA TGTCCCCACA CTCCTGGAGA AAGTGAGTTT GCAGGAGACA 
GTCCCAGATG CTTCCAGGGT TCCCAAGAAA AGGACCTCAC TCTTCTCCTC CTTCAGACTC 
AAAGACAAAT CTTTCGAGAG TTCCCTCCAA GAATCTGGAC CAAGAAAGGA CTCCCAAGAC 
CTCTTCAGCA GCCCCAAGGG GAAGGTGCAG CCCATGGGCA ATGCCCAGCC CCCAGAGAAG 
CAGGGGCAGC CAATCAGTAG CACCTGCCTG GGGCAGAGGG TCCATCCTGT TTCTCCAGAG 
AAAGATGCAA GTCCCACTCA CGTCCCCATC AGAGCGCAGG GGACAGGGAC TGTCTCTTCC 
ACCTCTTCCT CCACCACCTC CTCTGCAGAT GAGGAATTCC ATCCCCAGCC TTCCTCGCGG 
TCAAAGGAGA GGAAGGCACT TAGAAGAAGA AGGAAGCTGG AAAAAGCAAC AAAGCAATTG 
GTTAAGCAAG AAGAATTGAA AAGACTTCAT AAAGCTCAGG CCATCCAGAG GCAGTTGGAG 
GAAGTGGAGG AGCAGCAGAG GGCTTTCGAG ATCCAGGGCG TGAGGTGGGA GAAGGCGCTG 
AGAGGAGAAG CAGCAGATTC AGGCACACAG GATGAAGCAC AGCTTTTGCA GGAATGGCTT 
AAGCTGGTTC TGGAGAAGAA TAAATTAATG CGATATGAGT CAGAGCTCCT GATCATGGCC 
CACGAACTAG AACTGGAAGA TCATCAAAGC AGACTAGAGC AGAAATTAAG AGAGAAAATG 
CTCCAGGAAG AGAGCCAGAA AGATGAGAAT GATCTGAATG AAGAGCAAGA AATACTCACC 
GAGATGATGC AAGTGATTGA GCAAAGGGAC AAATTGGTGG ATTCCTTAGA GGAACAACGC 
ATCAGAGAGA AGGCCGAAGA CCAGCACTTT GAAAGCTTCA TATTCTCTAG GGGCTGTCAG 
CTGAGCAGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL2 ( XM_010812544.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810846.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa52589
Clone ID Related Accession (Same CDS sequence)	XM_015466507.1 , XM_010812544.2
Accession Version	XM_010812544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGATG AGCCGACCTC CCCCAAGAAG CCCAAGAGCA TCCCGGAGCC TGAGCCTGGG 
GATGTAGCAG GTGGAGCCGC CTCCCCCCTG CCCTCTGAGT GGACCGCAGT GAGAATCAGC 
CCCGGGGAGG ATGTGGTTGG ACAGGATGTG CTGGCCGTAC GCGTCCTGGT CACCAGCGAT 
GACAGCAGCT CCGATGCAGA ATTGGACCGT GGTGACAGTG AGGCCTCCAG TGTGGAGCCC 
TTTGATGAAA GACCCCAGCA ACCGGAACTC CAGCTCCCAC CCCTCCTGAA GCCCTTCACC 
CGGCACAGCT CCCTGAGGGA GGCCCTGCCC AGGGCAGTCT CTCCACATGA CCTCGGGGAG 
CCCGAAGCTG AGCCTGCTCT GCAGCGGGCC AATAGTTTCC AGTGTCCAAC TCCCAGCGAA 
TACCAGAGTG GGAGAAGATT CCAGTCCAAT TTCATGCCGG TGAACAGCAA AATGACCGTG 
TCGCCTCCAA AGCAAGCTTG TCCTCCCCCG CCTTCTCCTT CTCCACCCTC TTCTTCCTCG 
ATTGGTGACC TGAATGCCTT GGACAGTCCC TCCCTGCCTC AGGTGACAGT TTCCAACACC 
GCCTCACAAA TCTCCAGAGG TCACTGCAGT CCATCCACCC CCATCTTTCT GAGGCGTGCC 
AAAGCCCATG GACCACCCAA GGATCTCCCC CTGCGTCTGC CTCGTGGGCA GGTACTGGAG 
CGGACAGAAT ACTGCCTGGT GAGGCCTGGT GGAAATGGCC TCCAGAGCTC CAGGCCACCC 
AGCCCTACAG AGATGGCTTC TGATGGGTGT CAGGAGGCAG TGGCCCCTCC TAGGGACATC 
AGAAGCATCC ACAGAGGCCC ACACCCCGTT GAAGGGAAGG ACAGGTGCTT GCCAGACCAC 
CCACTGTCTC CCTGGGCTGG AGAGGAGCCT GGAGAAGGGA GTACCAGACC CAGGAGGGTA 
GAGGATGGTG GGTTAGAGCT GGCAGAGGAG AAGTCAGGCT TAAAGAAGTT GGTTCTCACT 
CCGGAGCAGA AGACTTGGTT GTTGGATTGG AACGACTCCA ACCTTGAGAA CTTGTACCTG 
GAAACCGGAG CACGACTTTC CCAAAAGAGC ACTAGAAATG GTTCAGGAGG CCATGTGCTG 
AAACCAGTCC ACCCCTTGCT GCTTCCTCTG GCAGAGAAAG AGACCTTGCC AGCCCAGAGA 
CAGTCTCAGG AGAAGATGGG GGCCCCAGCT GAACGGGCTC CTGGGGAACG AAGCATGGCC 
CCACCCAAGT CTCCACTGCG GCTTATAGCC AACGCCATCC GGAGATCCCT GGAGCCGCTC 
CTCCCCAACT CTGACAGTGG GAGGAAGGCC TGGGCCAAGC CAGAGTCAAA GACTTTGCCC 
ACCAGCCCAC CTCATGCTTA CACTTGCTCA TTCAGCTTTC GGAAAAGCAG CTCCAGTAAA 
GACAGCGATC AGCCATCCCC CAAGAGAGAC GTGGCAAGCA AGGCCTCAGC CTTCTTCTCC 
CTGGGCTCCC CGACTGCAAG GACTGCCCAG CCCTCAGCCC CGAGCCCTCC TGCCCCCGCC 
CTTTGCACTC ACAGTTTGCC CAGCCGGTCA TCCAAGGTGT TTCCTGCACT TGTGCCCCCA 
CCCTGTGGCA AGATGGAAGA TGTCCCCACA CTCCTGGAGA AAGTGAGTTT GCAGGAGACA 
GTCCCAGATG CTTCCAGGGT TCCCAAGAAA AGGACCTCAC TCTTCTCCTC CTTCAGACTC 
AAAGACAAAT CTTTCGAGAG TTCCCTCCAA GAATCTGGAC CAAGAAAGGA CTCCCAAGAC 
CTCTTCAGCA GCCCCAAGGG GAAGGTGCAG CCCATGGGCA ATGCCCAGCC CCCAGAGAAG 
CAGGGGCAGC CAATCAGTAG CACCTGCCTG GGGCAGAGGG TCCATCCTGT TTCTCCAGAG 
AAAGATGCAA GTCCCACTCA CGTCCCCATC AGAGCGCAGG GGACAGGGAC TGTCTCTTCC 
ACCTCTTCCT CCACCACCTC CTCTGCAGAT GAGGAATTCC ATCCCCAGCC TTCCTCGCGG 
TCAAAGGAGA GGAAGGCACT TAGAAGAAGA AGGAAGCTGG AAAAAGCAAC AAAGCAATTG 
GTTAAGCAAG AAGAATTGAA AAGACTTCAT AAAGCTCAGG CCATCCAGAG GCAGTTGGAG 
GAAGTGGAGG AGCAGCAGAG GGCTTTCGAG ATCCAGGGCG TGAGGTGGGA GAAGGCGCTG 
AGAGGAGAAG CAGCAGATTC AGGCACACAG GATGAAGCAC AGCTTTTGCA GGAATGGCTT 
AAGCTGGTTC TGGAGAAGAA TAAATTAATG CGATATGAGT CAGAGCTCCT GATCATGGCC 
CACGAACTAG AACTGGAAGA TCATCAAAGC AGACTAGAGC AGAAATTAAG AGAGAAAATG 
CTCCAGGAAG AGAGCCAGAA AGATGAGAAT GATCTGAATG AAGAGCAAGA AATACTCACC 
GAGATGATGC AAGTGATTGA GCAAAGGGAC AAATTGGTGG ATTCCTTAGA GGAACAACGC 
ATCAGAGAGA AGGCCGAAGA CCAGCACTTT GAAAGCTTCA TATTCTCTAG GGGCTGTCAG 
CTGAGCAGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810846.1
CDS	330..5504
Translation	MGENEDEKQTQAGQLFENFVQASTCKGTLQAFNILTRQLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGRSCMNTKCLIIGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALILGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPVDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKKETGIDLENIVYYKDCTHYFVMTAKRQSLLDKGVIINDYTDTETLLRAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTSMYASENAALVRERQSHLLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWDQGTSPLELLAERESLYRLLPQTTPENINKNFEQYTLDPATRYPNLNSNNIKPNQVKHLYITKELHQYPLERLGSVRRSVGLSRRESDVRPSKLLTWCQQQTEGYQHVNVTDLTTSWRSGLALCAIIHRFRPDLINFDSLNEDHAVENNQLAFDVAEREFGIAPVITGKEMASAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYEENADLGLAKSSIPHHYLNLTFPRKRTPRVDSRTEENDMNKRRRKGFNNLHEPSAFSSQSLGSNQEGIKEGGNQNKVKSMASQLLAKFEESSRNPSILRQESVRKTFPLNVGGSDTCYFCKKRVYVMERLSAEGRFFHRECFRCSVCATTLHLATYAFDVDEGKFFCKPHFIHCKTNSQQRKRRAELKQQKEEEGMWKEQEAARRDPPSESSCAAAAIATPEGSPPDEPTSPKKPKSIPEPEPGDVAGGAASPLPSEWTAVRISPGEDVVGQDVLAVRVLVTSDDSSSDAELDRGDSEASSVEPFDERPQQPELQLPPLLKPFTRHSSLREALPRAVSPHDLGEPEAEPALQRANSFQCPTPSEYQSGRRFQSNFMPVNSKMTVSPPKQACPPPPSPSPPSSSSIGDLNALDSPSLPQVTVSNTASQISRGHCSPSTPIFLRRAKAHGPPKDLPLRLPRGQVLERTEYCLVRPGGNGLQSSRPPSPTEMASDGCQEAVAPPRDIRSIHRGPHPVEGKDRCLPDHPLSPWAGEEPGEGSTRPRRVEDGGLELAEEKSGLKKLVLTPEQKTWLLDWNDSNLENLYLETGARLSQKSTRNGSGGHVLKPVHPLLLPLAEKETLPAQRQSQEKMGAPAERAPGERSMAPPKSPLRLIANAIRRSLEPLLPNSDSGRKAWAKPESKTLPTSPPHAYTCSFSFRKSSSSKDSDQPSPKRDVASKASAFFSLGSPTARTAQPSAPSPPAPALCTHSLPSRSSKVFPALVPPPCGKMEDVPTLLEKVSLQETVPDASRVPKKRTSLFSSFRLKDKSFESSLQESGPRKDSQDLFSSPKGKVQPMGNAQPPEKQGQPISSTCLGQRVHPVSPEKDASPTHVPIRAQGTGTVSSTSSSTTSSADEEFHPQPSSRSKERKALRRRRKLEKATKQLVKQEELKRLHKAQAIQRQLEEVEEQQRAFEIQGVRWEKALRGEAADSGTQDEAQLLQEWLKLVLEKNKLMRYESELLIMAHELELEDHQSRLEQKLREKMLQEESQKDENDLNEEQEILTEMMQVIEQRDKLVDSLEEQRIREKAEDQHFESFIFSRGCQLSRT

Target ORF information:

RefSeq Version	XM_010812544.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 2 (MICAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812544.2

ORF Insert Sequence:

ATGGGGGAGA ATGAGGATGA GAAGCAGACC CAGGCCGGGC AGTTATTCGA GAACTTTGTC 
CAAGCTTCCA CATGCAAAGG TACCCTTCAG GCCTTCAACA TCCTCACCCG ACAACTGGAC 
CTAGACCCTC TGGACCACAG AAACTTTTAT TCCAAGCTCA AGTCCAAAGT GACCACCTGG 
AAGGCCAAGG CCCTGTGGTA CAAACTTGAC AAGCGTGGGT CCCACAAAGA GTATAAGCGA 
GGAAGGTCTT GTATGAACAC CAAGTGTCTC ATCATTGGCG GAGGACCTTG TGGTCTGCGT 
ACTGCCATTG AACTTGCCTA CCTGGGGGCC AAAGTGGTCG TGGTGGAGAA GAGGGACACT 
TTCTCCCGGA ACAACGTGCT GCACCTCTGG CCTTTCACCA TCCATGACCT GCGAGGCCTG 
GGAGCCAAGA AGTTCTATGG GAAGTTTTGT GCTGGTTCCA TCGACCATAT CAGTATTCGT 
CAGTTGCAGC TCATCCTGTT CAAGGTGGCC TTGATATTGG GGGTTGAAAT CCATGTGAAT 
GTGGAGTTTG TGAAGGTTCT AGAGCCTCCT GAAGATCAAG AAAATCAAAA AATTGGCTGG 
CGGGCAGAAT TTCTCCCTGT AGATCACTCT CTGTCGGAAT TTGAGTTTGA TGTCATCATT 
GGCGCAGATG GCCGCAGGAA CACACTGGAA GGGTTCAGAA GAAAAGAATT CCGTGGGAAG 
CTGGCTATTG CAATCACCGC CAACTTCATC AACAGAAACA GCACAGCTGA GGCTAAGGTG 
GAAGAGATTA GCGGTGTGGC TTTCATCTTC AATCAGAAAT TTTTTCAGGA TCTTAAAAAA 
GAAACAGGGA TTGATCTTGA GAACATTGTT TACTACAAGG ACTGCACCCA CTATTTTGTC 
ATGACTGCCA AGAGGCAGAG CTTGCTTGAC AAAGGTGTCA TCATTAATGA CTATACCGAC 
ACAGAGACGC TGCTGCGCGC GGAGAATGTG AACCAGGACA ACCTGCTTTC CTACGCACGC 
GAGGCTGCAG ACTTCGCCAC CAATTACCAG CTGCCGTCTT TAGACTTTGC CATGAACCAT 
TACGGGCAGC CCGACGTGGC CATGTTCGAC TTCACCTCCA TGTACGCCTC CGAGAATGCG 
GCCTTGGTGC GCGAGCGGCA GTCACACCTG CTGCTCGTGG CCCTCGTGGG TGACAGCTTG 
CTTGAGCCAT TCTGGCCCAT GGGCACTGGC TGTGCCCGTG GCTTCCTGGC AGCCTTTGAC 
ACTGCATGGA TGGTGAAAAG CTGGGACCAG GGCACTTCTC CCCTGGAGCT TCTGGCTGAA 
AGAGAAAGTC TTTACCGACT GTTACCTCAA ACGACCCCAG AGAACATCAA CAAGAACTTT 
GAGCAGTACA CATTAGACCC GGCGACACGG TACCCAAACC TCAACTCAAA CAACATCAAG 
CCGAATCAGG TGAAGCATTT GTACATTACT AAGGAGCTGC ACCAGTATCC TCTTGAGAGG 
CTGGGCTCAG TGAGGAGATC CGTCGGCCTC TCCAGGCGAG AGTCAGACGT CAGGCCCAGC 
AAGCTCTTAA CCTGGTGCCA GCAGCAGACC GAGGGCTACC AGCATGTCAA TGTCACCGAC 
CTGACCACGT CCTGGCGGAG TGGCCTGGCC CTGTGTGCCA TCATCCACCG CTTCCGGCCT 
GACCTGATCA ACTTTGACTC TTTGAATGAA GACCACGCTG TGGAGAACAA CCAGCTGGCA 
TTTGACGTGG CGGAGCGTGA GTTTGGCATC GCCCCAGTGA TCACAGGCAA AGAGATGGCA 
TCGGCCCAGG AGCCTGACAA GCTCAGTATG GTCATGTACC TCTCCAAGTT CTACGAGCTC 
TTCCGGGGCA CCCCTCTGAG GCCTGTGGAT TCTTGGCGTA AAAACTATGA AGAGAATGCC 
GACCTCGGCT TGGCCAAGTC ATCCATTCCT CATCACTATC TCAACCTCAC GTTTCCGAGG 
AAGAGGACTC CGCGAGTGGA CAGTCGAACC GAAGAGAATG ACATGAACAA GCGGAGGCGG 
AAGGGCTTCA ACAACCTGCA CGAGCCTTCA GCCTTTTCCA GCCAGAGCTT GGGCTCCAAT 
CAAGAGGGCA TTAAGGAAGG TGGGAATCAG AACAAAGTCA AGTCCATGGC AAGTCAGCTG 
CTGGCCAAGT TCGAGGAGAG CTCACGGAAC CCCTCAATCC TGAGGCAGGA GTCCGTTCGG 
AAGACGTTTC CCCTGAACGT GGGTGGCAGC GACACCTGTT ACTTCTGCAA GAAGCGCGTG 
TACGTCATGG AGCGGCTGAG CGCGGAGGGC CGCTTCTTCC ACCGGGAGTG TTTCCGCTGC 
AGCGTCTGCG CCACCACCTT GCACCTGGCC ACCTACGCCT TCGACGTTGA CGAAGGCAAG 
TTTTTCTGCA AGCCTCATTT CATTCACTGT AAGACCAACA GCCAGCAACG GAAGAGACGG 
GCAGAGTTGA AGCAACAAAA AGAGGAGGAG GGGATGTGGA AAGAGCAAGA AGCCGCTAGG 
CGAGACCCTC CCTCGGAAAG TTCTTGCGCT GCCGCTGCCA TCGCCACGCC GGAAGGCAGC 
CCCCCAGATG AGCCGACCTC CCCCAAGAAG CCCAAGAGCA TCCCGGAGCC TGAGCCTGGG 
GATGTAGCAG GTGGAGCCGC CTCCCCCCTG CCCTCTGAGT GGACCGCAGT GAGAATCAGC 
CCCGGGGAGG ATGTGGTTGG ACAGGATGTG CTGGCCGTAC GCGTCCTGGT CACCAGCGAT 
GACAGCAGCT CCGATGCAGA ATTGGACCGT GGTGACAGTG AGGCCTCCAG TGTGGAGCCC 
TTTGATGAAA GACCCCAGCA ACCGGAACTC CAGCTCCCAC CCCTCCTGAA GCCCTTCACC 
CGGCACAGCT CCCTGAGGGA GGCCCTGCCC AGGGCAGTCT CTCCACATGA CCTCGGGGAG 
CCCGAAGCTG AGCCTGCTCT GCAGCGGGCC AATAGTTTCC AGTGTCCAAC TCCCAGCGAA 
TACCAGAGTG GGAGAAGATT CCAGTCCAAT TTCATGCCGG TGAACAGCAA AATGACCGTG 
TCGCCTCCAA AGCAAGCTTG TCCTCCCCCG CCTTCTCCTT CTCCACCCTC TTCTTCCTCG 
ATTGGTGACC TGAATGCCTT GGACAGTCCC TCCCTGCCTC AGGTGACAGT TTCCAACACC 
GCCTCACAAA TCTCCAGAGG TCACTGCAGT CCATCCACCC CCATCTTTCT GAGGCGTGCC 
AAAGCCCATG GACCACCCAA GGATCTCCCC CTGCGTCTGC CTCGTGGGCA GGTACTGGAG 
CGGACAGAAT ACTGCCTGGT GAGGCCTGGT GGAAATGGCC TCCAGAGCTC CAGGCCACCC 
AGCCCTACAG AGATGGCTTC TGATGGGTGT CAGGAGGCAG TGGCCCCTCC TAGGGACATC 
AGAAGCATCC ACAGAGGCCC ACACCCCGTT GAAGGGAAGG ACAGGTGCTT GCCAGACCAC 
CCACTGTCTC CCTGGGCTGG AGAGGAGCCT GGAGAAGGGA GTACCAGACC CAGGAGGGTA 
GAGGATGGTG GGTTAGAGCT GGCAGAGGAG AAGTCAGGCT TAAAGAAGTT GGTTCTCACT 
CCGGAGCAGA AGACTTGGTT GTTGGATTGG AACGACTCCA ACCTTGAGAA CTTGTACCTG 
GAAACCGGAG CACGACTTTC CCAAAAGAGC ACTAGAAATG GTTCAGGAGG CCATGTGCTG 
AAACCAGTCC ACCCCTTGCT GCTTCCTCTG GCAGAGAAAG AGACCTTGCC AGCCCAGAGA 
CAGTCTCAGG AGAAGATGGG GGCCCCAGCT GAACGGGCTC CTGGGGAACG AAGCATGGCC 
CCACCCAAGT CTCCACTGCG GCTTATAGCC AACGCCATCC GGAGATCCCT GGAGCCGCTC 
CTCCCCAACT CTGACAGTGG GAGGAAGGCC TGGGCCAAGC CAGAGTCAAA GACTTTGCCC 
ACCAGCCCAC CTCATGCTTA CACTTGCTCA TTCAGCTTTC GGAAAAGCAG CTCCAGTAAA 
GACAGCGATC AGCCATCCCC CAAGAGAGAC GTGGCAAGCA AGGCCTCAGC CTTCTTCTCC 
CTGGGCTCCC CGACTGCAAG GACTGCCCAG CCCTCAGCCC CGAGCCCTCC TGCCCCCGCC 
CTTTGCACTC ACAGTTTGCC CAGCCGGTCA TCCAAGGTGT TTCCTGCACT TGTGCCCCCA 
CCCTGTGGCA AGATGGAAGA TGTCCCCACA CTCCTGGAGA AAGTGAGTTT GCAGGAGACA 
GTCCCAGATG CTTCCAGGGT TCCCAAGAAA AGGACCTCAC TCTTCTCCTC CTTCAGACTC 
AAAGACAAAT CTTTCGAGAG TTCCCTCCAA GAATCTGGAC CAAGAAAGGA CTCCCAAGAC 
CTCTTCAGCA GCCCCAAGGG GAAGGTGCAG CCCATGGGCA ATGCCCAGCC CCCAGAGAAG 
CAGGGGCAGC CAATCAGTAG CACCTGCCTG GGGCAGAGGG TCCATCCTGT TTCTCCAGAG 
AAAGATGCAA GTCCCACTCA CGTCCCCATC AGAGCGCAGG GGACAGGGAC TGTCTCTTCC 
ACCTCTTCCT CCACCACCTC CTCTGCAGAT GAGGAATTCC ATCCCCAGCC TTCCTCGCGG 
TCAAAGGAGA GGAAGGCACT TAGAAGAAGA AGGAAGCTGG AAAAAGCAAC AAAGCAATTG 
GTTAAGCAAG AAGAATTGAA AAGACTTCAT AAAGCTCAGG CCATCCAGAG GCAGTTGGAG 
GAAGTGGAGG AGCAGCAGAG GGCTTTCGAG ATCCAGGGCG TGAGGTGGGA GAAGGCGCTG 
AGAGGAGAAG CAGCAGATTC AGGCACACAG GATGAAGCAC AGCTTTTGCA GGAATGGCTT 
AAGCTGGTTC TGGAGAAGAA TAAATTAATG CGATATGAGT CAGAGCTCCT GATCATGGCC 
CACGAACTAG AACTGGAAGA TCATCAAAGC AGACTAGAGC AGAAATTAAG AGAGAAAATG 
CTCCAGGAAG AGAGCCAGAA AGATGAGAAT GATCTGAATG AAGAGCAAGA AATACTCACC 
GAGATGATGC AAGTGATTGA GCAAAGGGAC AAATTGGTGG ATTCCTTAGA GGAACAACGC 
ATCAGAGAGA AGGCCGAAGA CCAGCACTTT GAAAGCTTCA TATTCTCTAG GGGCTGTCAG 
CTGAGCAGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MICAL2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: