PHLDB1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHLDB1
Entrez Gene ID	533996
Full Name	pleckstrin homology like domain family B member 1
General protein information	Preferred Names pleckstrin homology like domain family B member 1 Names pleckstrin homology-like domain family B member 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010812483.2	XP_010810785.1	pleckstrin homology-like domain family B member 1 isoform X2
XM_010812485.2	XP_010810787.1	pleckstrin homology-like domain family B member 1 isoform X4
XM_005215996.4	XP_005216053.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_024975344.1	XP_024831112.1	pleckstrin homology-like domain family B member 1 isoform X4
XM_010812485.3	XP_010810787.1	pleckstrin homology-like domain family B member 1 isoform X5
XM_005216005.4	XP_005216062.1	pleckstrin homology-like domain family B member 1 isoform X12
XM_010812482.2	XP_010810784.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_005215999.3	XP_005216056.1	pleckstrin homology-like domain family B member 1 isoform X6
XM_010812483.3	XP_010810785.1	pleckstrin homology-like domain family B member 1 isoform X2
XM_005216002.4	XP_005216059.1	pleckstrin homology-like domain family B member 1 isoform X8
XM_005216006.4	XP_005216063.1	pleckstrin homology-like domain family B member 1 isoform X13
XM_010812487.3	XP_010810789.1	pleckstrin homology-like domain family B member 1 isoform X14
XM_005216007.3	XP_005216064.1	pleckstrin homology-like domain family B member 1 isoform X15
XM_005216008.4	XP_005216065.1	pleckstrin homology-like domain family B member 1 isoform X16
XM_005216002.3	XP_005216059.1	pleckstrin homology-like domain family B member 1 isoform X7
XM_010812486.1	XP_010810788.1	pleckstrin homology-like domain family B member 1 isoform X8
XM_005216003.3	XP_005216060.1	pleckstrin homology-like domain family B member 1 isoform X9
XM_005216004.3	XP_005216061.1	pleckstrin homology-like domain family B member 1 isoform X10
XM_010812487.2	XP_010810789.1	pleckstrin homology-like domain family B member 1 isoform X13
XM_015474658.2	XP_015330144.1	pleckstrin homology-like domain family B member 1 isoform X6
XM_005216003.4	XP_005216060.1	pleckstrin homology-like domain family B member 1 isoform X10
XM_015474658.1	XP_015330144.1	pleckstrin homology-like domain family B member 1 isoform X5
XM_010812484.2	XP_010810786.1	pleckstrin homology-like domain family B member 1 isoform X3
XM_005216006.3	XP_005216063.1	pleckstrin homology-like domain family B member 1 isoform X12
XM_005216007.2	XP_005216064.1	pleckstrin homology-like domain family B member 1 isoform X14
NM_001206042.1	NP_001192971.1	pleckstrin homology-like domain family B member 1
XM_010812482.3	XP_010810784.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_005215999.4	XP_005216056.1	pleckstrin homology-like domain family B member 1 isoform X7
XM_010812486.2	XP_010810788.1	pleckstrin homology-like domain family B member 1 isoform X9
XM_005216004.4	XP_005216061.1	pleckstrin homology-like domain family B member 1 isoform X11
NM_001206042.2	NP_001192971.1	pleckstrin homology-like domain family B member 1
XM_005215996.3	XP_005216053.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_005216005.3	XP_005216062.1	pleckstrin homology-like domain family B member 1 isoform X11
XM_005216008.3	XP_005216065.1	pleckstrin homology-like domain family B member 1 isoform X15
XM_010812484.3	XP_010810786.1	pleckstrin homology-like domain family B member 1 isoform X3
XM_024975347.1	XP_024831115.1	pleckstrin homology-like domain family B member 1 isoform X17

Pathways from BioSystems

Homologies

Homo sapiens (human)	PHLDB1	NP_001138231.1
Gallus gallus (chicken)	PHLDB1	XP_417927.4
Danio rerio (zebrafish)	LOC570275	XP_005157508.1
Xenopus tropicalis (tropical clawed frog)	phldb1	NP_001123804.1
Canis lupus familiaris (dog)	PHLDB1	XP_005619762.1
Bos taurus (cattle)	PHLDB1	NP_001192971.1
Rattus norvegicus (Norway rat)	Phldb1	NP_001178507.1
Pan troglodytes (chimpanzee)	PHLDB1	XP_001162204.1
Mus musculus (house mouse)	Phldb1	NP_705765.3
	LOC706336	XP_002808134.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHLDB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa239028	NM_001206042.2 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222841	XM_015474658.2 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222842	XM_005215999.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222843	XM_005216002.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222844	XM_010812486.2 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222845	XM_005216003.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222846	XM_005216004.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222847	XM_005216005.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222848	XM_005216006.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222849	XM_010812487.3 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222850	XM_005216007.3 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222851	XM_005216008.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222852	XM_024975347.1 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222836	XM_005215996.4 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222836	XM_010812482.3 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222837	XM_010812483.3 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222838	XM_010812484.3 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222839	XM_024975344.1 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222840	XM_010812485.3 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa37883	NM_001206042.1 Latest version!	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26349	XM_005215996.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa26349	XM_010812482.2 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa26352	XM_005215999.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26355	XM_005216002.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26356	XM_005216003.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26357	XM_005216004.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26358	XM_005216005.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26359	XM_005216006.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26360	XM_005216007.2 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa26361	XM_005216008.3 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa52569	XM_010812483.2 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa52570	XM_010812484.2 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa52571	XM_010812485.2 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa52572	XM_010812486.1 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa52573	XM_010812487.2 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa92656	XM_015474658.1 Latest version!	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHLDB1 ( NM_001206042.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192971.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.

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CloneID	OBa239028
Clone ID Related Accession (Same CDS sequence)	NM_001206042.2
Accession Version	NM_001206042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On May 30, 2018 this sequence version replaced NM_001206042.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571258.2763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC 
ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192971.1
CDS	378..4520
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	NM_001206042.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206042.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC 
ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_015474658.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330144.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.

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CloneID	OBa222841
Clone ID Related Accession (Same CDS sequence)	XM_015474658.2
Accession Version	XM_015474658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG 
ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG 
GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC 
GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG 
CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG 
GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC 
CAGCTGCAGG TTTACCGCTC CAAAATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC 
CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG 
GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC 
AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC 
CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330144.1
CDS	392..4882
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_015474658.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474658.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG 
ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG 
GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC 
GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG 
CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG 
GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC 
CAGCTGCAGG TTTACCGCTC CAAAATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC 
CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG 
GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC 
AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC 
CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005215999.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216056.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.

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CloneID	OBa222842
Clone ID Related Accession (Same CDS sequence)	XM_005215999.4
Accession Version	XM_005215999.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215999.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAAATGGAT 
GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC 
TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG 
AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG 
CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC 
GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG 
CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC 
GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC 
TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG 
GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT 
GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA 
AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG 
CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC 
GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG 
TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG 
GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC 
TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG 
AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG 
GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC 
TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216056.1
CDS	392..4852
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005215999.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215999.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAAATGGAT 
GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC 
TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG 
AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG 
CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC 
GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG 
CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC 
GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC 
TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG 
GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT 
GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA 
AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG 
CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC 
GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG 
TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG 
GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC 
TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG 
AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG 
GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC 
TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216002.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216059.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa222843
Clone ID Related Accession (Same CDS sequence)	XM_005216002.4
Accession Version	XM_005216002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216002.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA 
GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC 
GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG 
CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG 
GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC 
ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT 
GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC 
GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC 
CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC 
CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA 
CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC 
GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC 
CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC 
TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG 
GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC 
AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC 
TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216059.1
CDS	391..4707
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216002.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216002.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA 
GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC 
GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG 
CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG 
GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC 
ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT 
GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC 
GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC 
CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC 
CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA 
CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC 
GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC 
CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC 
TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG 
GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC 
AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC 
TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812486.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810788.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.

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CloneID	OBa222844
Clone ID Related Accession (Same CDS sequence)	XM_010812486.2
Accession Version	XM_010812486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810788.1
CDS	149..4423
Translation	MLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812486.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812486.2

ORF Insert Sequence:

ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216003.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216060.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.

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CloneID	OBa222845
Clone ID Related Accession (Same CDS sequence)	XM_005216003.4
Accession Version	XM_005216003.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216003.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG 
CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA 
CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT 
GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG 
CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG 
CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG 
CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG 
CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC 
AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC 
CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC 
CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG 
TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216060.1
CDS	391..4662
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216003.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216003.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG 
CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA 
CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT 
GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG 
CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG 
CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG 
CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG 
CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC 
AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC 
CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC 
CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG 
TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216004.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216061.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.

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CloneID	OBa222846
Clone ID Related Accession (Same CDS sequence)	XM_005216004.4
Accession Version	XM_005216004.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG 
CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG 
GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC 
TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC 
GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC 
AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC 
CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG 
AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG 
GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG 
CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG 
ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA 
GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC 
TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG 
CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC 
TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG 
TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG 
ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG 
GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216061.1
CDS	391..4578
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216004.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216004.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG 
CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG 
GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC 
TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC 
GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC 
AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC 
CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG 
AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG 
GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG 
CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG 
ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA 
GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC 
TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG 
CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC 
TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG 
TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG 
ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG 
GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216005.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216062.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa222847
Clone ID Related Accession (Same CDS sequence)	XM_005216005.4
Accession Version	XM_005216005.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216005.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC 
GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG 
GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG 
TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC 
ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG 
CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG 
GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG 
AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG 
GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG 
CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG 
AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG 
CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG 
GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG 
GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC 
CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC 
ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC 
ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG 
CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC 
ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216062.1
CDS	391..4566
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216005.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216005.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC 
GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG 
GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG 
TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC 
ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG 
CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG 
GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG 
AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG 
GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG 
CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG 
AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG 
CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG 
GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG 
GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC 
CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC 
ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC 
ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG 
CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC 
ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216006.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216063.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.

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CloneID	OBa222848
Clone ID Related Accession (Same CDS sequence)	XM_005216006.4
Accession Version	XM_005216006.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216063.1
CDS	391..4521
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216006.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216006.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812487.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810789.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.

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CloneID	OBa222849
Clone ID Related Accession (Same CDS sequence)	XM_010812487.3
Accession Version	XM_010812487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810789.1
CDS	390..4499
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812487.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812487.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216007.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216064.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa222850
Clone ID Related Accession (Same CDS sequence)	XM_005216007.3
Accession Version	XM_005216007.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT 
CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG 
CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC 
CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC 
TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC 
GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC 
TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG 
GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG 
GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG 
GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC 
AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG 
AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT 
CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG 
ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT 
TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG 
GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG 
ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC 
ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA 
GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216064.1
CDS	390..4436
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216007.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216007.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT 
CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG 
CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC 
CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC 
TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC 
GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC 
TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG 
GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG 
GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG 
GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC 
AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG 
AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT 
CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG 
ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT 
TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG 
GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG 
ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC 
ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA 
GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216008.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216065.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.

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CloneID	OBa222851
Clone ID Related Accession (Same CDS sequence)	XM_005216008.4
Accession Version	XM_005216008.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216008.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG 
ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG 
TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG 
CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG 
GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC 
TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC 
ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA 
CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG 
AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT 
GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT 
TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT 
GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC 
AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG 
CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC 
CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG 
GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA 
GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT 
ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216065.1
CDS	390..4358
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216008.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216008.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG 
ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG 
TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG 
CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG 
GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC 
TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC 
ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA 
CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG 
AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT 
GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT 
TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT 
GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC 
AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG 
CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC 
CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG 
GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA 
GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT 
ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_024975347.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831115.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X18, mRNA.

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CloneID	OBa222852
Clone ID Related Accession (Same CDS sequence)	XM_024975347.1
Accession Version	XM_024975347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC 
ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831115.1
CDS	148..3930
Translation	MLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_024975347.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975347.1

ORF Insert Sequence:

ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC 
ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005215996.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216053.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222836
Clone ID Related Accession (Same CDS sequence)	XM_005215996.4 , XM_010812482.3
Accession Version	XM_005215996.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215996.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216053.1
CDS	392..5026
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005215996.4
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215996.4

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812482.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810784.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa222836
Clone ID Related Accession (Same CDS sequence)	XM_005215996.4 , XM_010812482.3
Accession Version	XM_010812482.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812482.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810784.1
CDS	278..4912
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812482.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812482.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812483.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810785.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.

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CloneID	OBa222837
Clone ID Related Accession (Same CDS sequence)	XM_010812483.3
Accession Version	XM_010812483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC 
GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC 
ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810785.1
CDS	392..4993
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812483.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812483.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC 
GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC 
ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812484.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810786.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.

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CloneID	OBa222838
Clone ID Related Accession (Same CDS sequence)	XM_010812484.3
Accession Version	XM_010812484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT 
GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC 
TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC 
AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT 
GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG 
AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG 
CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG 
GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC 
CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC 
TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC 
AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG 
GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810786.1
CDS	392..4981
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812484.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812484.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT 
GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC 
TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC 
AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT 
GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG 
AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG 
CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG 
GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC 
CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC 
TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC 
AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG 
GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_024975344.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831112.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.

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CloneID	OBa222839
Clone ID Related Accession (Same CDS sequence)	XM_024975344.1
Accession Version	XM_024975344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT 
GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC 
TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC 
AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT 
GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG 
AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG 
CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG 
GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC 
CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC 
TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC 
AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAGCC CGAACCCGGC CCTCACCTTC 
TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831112.1
CDS	392..4948
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_024975344.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975344.1

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT 
GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC 
TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC 
AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT 
GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG 
AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG 
CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG 
GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC 
CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC 
TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC 
AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAGCC CGAACCCGGC CCTCACCTTC 
TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812485.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810787.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa222840
Clone ID Related Accession (Same CDS sequence)	XM_010812485.3
Accession Version	XM_010812485.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC 
TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT 
CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA 
GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC 
ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC 
CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAAA TGGATGGCGA GGCCACCAGC 
CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC 
TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC 
CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC 
GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG 
CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT 
CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC 
ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA 
GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC 
GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC 
TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC 
AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC 
AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC 
CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810787.1
CDS	392..4897
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812485.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812485.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC 
TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT 
CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA 
GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC 
ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC 
CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAAA TGGATGGCGA GGCCACCAGC 
CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC 
TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC 
CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC 
GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG 
CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT 
CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC 
ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA 
GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC 
GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC 
TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC 
AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC 
AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC 
CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( NM_001206042.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192971.1 Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.

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CloneID	OBa37883
Clone ID Related Accession (Same CDS sequence)	NM_001206042.1
Accession Version	NM_001206042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02040474.1. On Apr 20, 2011 this sequence version replaced XM_613593.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822089, SAMN02822090 [ECO:0000348] ##Evidence-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC 
ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192971.1
CDS	378..4520
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	NM_001206042.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206042.1

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC 
ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812482.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810784.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.

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CloneID	OBa26349
Clone ID Related Accession (Same CDS sequence)	XM_010812482.2 , XM_005215996.3
Accession Version	XM_010812482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810784.1
CDS	281..4915
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812482.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812482.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005215996.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216053.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.

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CloneID	OBa26349
Clone ID Related Accession (Same CDS sequence)	XM_010812482.2 , XM_005215996.3
Accession Version	XM_005215996.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216053.1
CDS	395..5029
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005215996.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215996.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005215999.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216056.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa26352
Clone ID Related Accession (Same CDS sequence)	XM_005215999.3
Accession Version	XM_005215999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAGATGGAT 
GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC 
TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG 
AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG 
CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC 
GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG 
CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC 
GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC 
TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG 
GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT 
GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA 
AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG 
CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC 
GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG 
TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG 
GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC 
TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG 
AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG 
GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC 
TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216056.1
CDS	395..4855
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005215999.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215999.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAGATGGAT 
GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC 
TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG 
AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG 
CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC 
GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG 
CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC 
GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC 
TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG 
GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT 
GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA 
AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG 
CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC 
GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG 
TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG 
GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC 
TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG 
AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG 
GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC 
TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216002.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216059.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa26355
Clone ID Related Accession (Same CDS sequence)	XM_005216002.3
Accession Version	XM_005216002.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216002.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA 
GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC 
GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG 
CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG 
GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC 
ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT 
GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC 
GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC 
CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC 
CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA 
CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC 
GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC 
CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC 
TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG 
GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC 
AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC 
TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216059.1
CDS	394..4710
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216002.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216002.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA 
GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC 
GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG 
CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG 
GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC 
ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT 
GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC 
GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC 
CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC 
CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA 
CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC 
GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC 
CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC 
TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG 
GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC 
AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC 
TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216003.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216060.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.

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CloneID	OBa26356
Clone ID Related Accession (Same CDS sequence)	XM_005216003.3
Accession Version	XM_005216003.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG 
CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA 
CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT 
GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG 
CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG 
CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG 
CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG 
CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC 
AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC 
CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC 
CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG 
TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216060.1
CDS	394..4665
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216003.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216003.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC 
CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC 
AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC 
ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG 
CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA 
CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT 
GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG 
CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG 
CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG 
CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG 
CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC 
AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC 
CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC 
CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG 
TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216004.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216061.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa26357
Clone ID Related Accession (Same CDS sequence)	XM_005216004.3
Accession Version	XM_005216004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG 
CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG 
GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC 
TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC 
GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC 
AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC 
CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG 
AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG 
GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG 
CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG 
ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA 
GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC 
TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG 
CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC 
TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG 
TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG 
ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG 
GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216061.1
CDS	393..4580
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216004.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216004.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG 
CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG 
GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC 
TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC 
GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC 
AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC 
CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG 
AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG 
GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG 
CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG 
ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA 
GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC 
TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG 
CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC 
TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG 
TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG 
ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG 
GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216005.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216062.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa26358
Clone ID Related Accession (Same CDS sequence)	XM_005216005.3
Accession Version	XM_005216005.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216005.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC 
GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG 
GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG 
TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC 
ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG 
CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG 
GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG 
AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG 
GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG 
CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG 
AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG 
CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG 
GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG 
GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC 
CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC 
ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC 
ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG 
CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC 
ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216062.1
CDS	393..4568
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216005.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216005.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC 
GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG 
GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG 
TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC 
ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG 
CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG 
GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG 
AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG 
GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG 
CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG 
AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG 
CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG 
GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG 
GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC 
CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC 
ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC 
ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG 
CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC 
ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216006.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216063.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.

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CloneID	OBa26359
Clone ID Related Accession (Same CDS sequence)	XM_005216006.3
Accession Version	XM_005216006.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216063.1
CDS	393..4523
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216006.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216006.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC 
CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC 
AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC 
CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216007.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216064.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X15, mRNA.

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CloneID	OBa26360
Clone ID Related Accession (Same CDS sequence)	XM_005216007.2
Accession Version	XM_005216007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT 
CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG 
CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC 
CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC 
TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC 
GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC 
TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG 
GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG 
GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG 
GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC 
AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG 
AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT 
CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG 
ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT 
TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG 
GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG 
ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC 
ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA 
GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216064.1
CDS	393..4439
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216007.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216007.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT 
CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG 
CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC 
CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC 
TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC 
GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC 
TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG 
GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG 
GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG 
GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC 
AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG 
AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT 
CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG 
ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT 
TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG 
GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG 
ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC 
ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA 
GAGGGCTACA CTCAGTTTAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005216008.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216065.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X16, mRNA.

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CloneID	OBa26361
Clone ID Related Accession (Same CDS sequence)	XM_005216008.3
Accession Version	XM_005216008.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG 
ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG 
TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG 
CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG 
GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC 
TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC 
ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA 
CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG 
AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT 
GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT 
TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT 
GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC 
AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG 
CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC 
CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG 
GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA 
GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT 
ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216065.1
CDS	392..4360
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005216008.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216008.3

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG 
ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC 
TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA 
CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC 
ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG 
ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG 
TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG 
CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG 
GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC 
TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC 
ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA 
CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG 
AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT 
GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT 
TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT 
GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC 
AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG 
CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC 
CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG 
GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA 
GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT 
ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812483.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810785.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa52569
Clone ID Related Accession (Same CDS sequence)	XM_010812483.2
Accession Version	XM_010812483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC 
GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC 
ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810785.1
CDS	395..4996
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812483.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812483.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC 
GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC 
ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812484.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810786.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.

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CloneID	OBa52570
Clone ID Related Accession (Same CDS sequence)	XM_010812484.2
Accession Version	XM_010812484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT 
GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC 
TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC 
AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT 
GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG 
AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG 
CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG 
GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC 
CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC 
TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC 
AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG 
GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810786.1
CDS	395..4984
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812484.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812484.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT 
GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC 
TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC 
AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT 
GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG 
AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG 
CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG 
GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC 
CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC 
TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC 
AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG 
GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812485.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810787.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.

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CloneID	OBa52571
Clone ID Related Accession (Same CDS sequence)	XM_010812485.2
Accession Version	XM_010812485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC 
TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT 
CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA 
GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC 
ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC 
CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGCGA GGCCACCAGC 
CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC 
TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC 
CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC 
GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG 
CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT 
CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC 
ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA 
GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC 
GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC 
TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC 
AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC 
AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC 
CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810787.1
CDS	395..4900
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812485.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812485.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC 
TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT 
CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA 
GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC 
ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC 
CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGCGA GGCCACCAGC 
CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC 
TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC 
CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC 
GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG 
CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT 
CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC 
ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA 
GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC 
GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC 
TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC 
AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC 
AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC 
CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812486.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810788.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa52572
Clone ID Related Accession (Same CDS sequence)	XM_010812486.1
Accession Version	XM_010812486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810788.1
CDS	148..4422
Translation	MLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEADLLISESSEVGLGTAALGPFPESSQAGASSLPLTPPASTQLCLKAQEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812486.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812486.1

ORF Insert Sequence:

ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT 
CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA 
GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC 
TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG 
CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG 
CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG 
GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG 
TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT 
CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC 
ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC 
CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG 
GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG 
GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC 
CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG 
ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG 
GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG 
TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG 
AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG 
CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG 
CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC 
CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG 
AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT 
CAGTTTATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_010812487.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810789.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa52573
Clone ID Related Accession (Same CDS sequence)	XM_010812487.2
Accession Version	XM_010812487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810789.1
CDS	393..4502
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_010812487.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812487.2

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC 
CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC 
CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG 
CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG 
ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG 
CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT 
ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG 
CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC 
CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG 
CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC 
AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT 
ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC 
CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG 
CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG 
AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC 
ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC 
TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG 
TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA 
GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG 
AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA 
CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG 
GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT 
GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC 
TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG 
GCAGAGGGCT ACACTCAGTT TATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_015474658.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330144.1 Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.

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CloneID	OBa92656
Clone ID Related Accession (Same CDS sequence)	XM_015474658.1
Accession Version	XM_015474658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	pleckstrin homology-like domain family B member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG 
ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG 
GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC 
GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG 
CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG 
GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC 
CAGCTGCAGG TTTACCGCTC CAAGATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC 
CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG 
GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC 
AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC 
CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330144.1
CDS	395..4885
Translation	MDTLNRNQVGPGCKTPGLVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGCMLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGPESESLVNGNHTPQHASRGPSACGSHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSLPPLVPARSSSYHLGLQPPQSRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRPGGQLPVVAISLSEYPASGARGPPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPGAERVLTTSPSRQLVGRTFSDGSATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPVSTRTAPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEAAAALALAGRRPSRGLAAGTGAPGRGSEEPGGAAQRLWESVERSDEENLKEECSSTESTQQEHEDAPGTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETSIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKERLVVLDSQAGQIRSQAVQESEHLARDKNAALQLLQKEKEKLAMLERRYHSLTGGRPFPKTTSTLKEYVTLEQLKVMWGTSPVPTGPAPGLPPWAPASQDLVPTTCLPPALPSSSSFASVTPSPQMEKLLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSTLLAQNGTSSLPRNLAATLQDIETKRQLALQQKVESLPAEPLPTDDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGVAPFPTGPAGFPPLMHHSILHHLPASRERGEEGEHAYDTLSLESSDSMETSISTGGNSVCSPDNMSSASGLDMGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKKRFLSFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_015474658.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474658.1

ORF Insert Sequence:

ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG 
GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG 
GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC 
AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC 
ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA 
GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT 
GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG 
GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA 
CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC 
ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA 
GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG 
CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC 
CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG 
AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG 
GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG 
ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC 
AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC 
CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC 
TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT 
CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG 
AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC 
AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT 
TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG 
CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG 
GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG 
AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG 
GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG 
CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG 
TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG 
GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC 
ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG 
AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC 
CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA 
GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG 
GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG 
GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG 
GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG 
CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG 
CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG 
GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG 
GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG 
GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT 
GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC 
CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG 
ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG 
GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC 
GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG 
CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG 
GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC 
CAGCTGCAGG TTTACCGCTC CAAGATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC 
CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG 
GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC 
AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC 
CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT 
GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG 
GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA 
GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC 
GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG 
AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG 
CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC 
CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC 
TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG 
AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG 
AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PHLDB1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: