CNOT1 cDNA ORF clone, Bos taurus(cattle)

The following CNOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa93372 XM_015475761.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa225087 XM_024978367.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225088 XM_024978368.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225089 XM_024978369.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225090 XM_024978370.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225091 XM_024978371.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225092 XM_024978372.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225093 XM_024978373.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225094 XM_024978374.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225095 XM_024978375.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa40207 NM_001206039.1
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa28465 XM_005218664.4
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa28467 XM_005218666.4
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa28468 XM_005218667.4
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa52564 XM_010814532.3
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa52565 XM_010814533.2
Latest version!
Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OBa52564 XM_010814532.2 Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa28465 XM_005218664.3 Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa28468 XM_005218667.3 Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa28467 XM_005218666.3 Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa93372
    Clone ID Related Accession (Same CDS sequence) XM_015475761.1
    Accession Version XM_015475761.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATGCAC CAGCAAGGAA TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331247.1
    CDS316..5751
    Translation

    Target ORF information:

    RefSeq Version XM_015475761.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATGCAC CAGCAAGGAA TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225087
    Clone ID Related Accession (Same CDS sequence) XM_024978367.1
    Accession Version XM_024978367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGAGTAAGAT GAAACCTTCG
    GACTTGTCTC AGGTGTGGCC AGAGGCAAAT CAGCACTTTA GTAAAGAGAT AGATGATGAA
    GCAAACAGCT ATTTCCAGAG AATATATAAT CACCCACCAC ATCCAACCAT GTCTGTTGAT
    GAGGTATTAG AAATGCTACA GAGATTTAAA GATTCTACCA TAAAGAGGGA ACGAGAAGTA
    TTTAACTGTA TGCTAAGGAA CTTGTTCGAA GAATATCGGT TCTTCCCCCA ATACCCTGAT
    AAAGAGTTAC ATATAACAGC CTGCCTGTTT GGTGGAATAA TCGAAAAAGG ACTGGTCACT
    TATATGGCAC TAGGTTTGGC CCTGCGATAC GTTCTTGAAG CCTTACGCAA GCCTTTTGGA
    TCCAAAATGT ATTATTTTGG GATTGCTGCA CTAGATAGAT TTAAAAATAG ATTGAAGGAC
    TATCCCCAGT ATTGTCAGCA CTTGGCTTCG ATCAGTCACT TTATGCAATT TCCACATCAT
    TTACAGGAGT ATATTGAGTA TGGACAGCAA TCTAGAGATC CTCCTGTGAA AATGCAGGGC
    TCTATCACAA CCCCTGGAAG TATTGCACTG GCTCAAGCCC AGGCCCAGGC CCAGGTTCCA
    GCAAAAGCTC CCCTTGCTGG CCAGGTTAGC ACTATGGTAA CCACCTCAAC AACCACCACT
    GTTGCTAAAA CGGTTACAGT CACCAGGCCA ACTGGAGTCA GCTTTAAGAA AGATGTGCCA
    CCTTCTATTA ATACAACAAA TATAGATACA TTGCTTGTGG CCACAGATCA AACTGAGAGA
    ATCGTAGAGC CCCCAGAAAA TATCCAGGAG AAAATTGCTT TTATCTTCAA CAACCTTTCC
    CAGTCAAATA TGACACAAAA GGTTGAAGAA CTAAAGGAAA CTGTGAAGGA AGAATTTATG
    CCTTGGGTTT CACAGTACCT GGTTATGAAG AGAGTCAGTA TTGAGCCAAA CTTTCACAGC
    CTGTATTCTA ACTTCCTTGA CACGCTGAAG AATCCTGAGT TTAATAAGAT GGTTCTGAAT
    GAGACCTATA GAAACATCAA AGTACTCCTG ACCTCTGATA AAGCTGCAGC CAATTTCTCT
    GATCGGTCTT TGCTGAAGAA CTTGGGACAT TGGCTAGGAA TGATTACACT AGCTAAAAAT
    AAACCCATCT TACACACTGA CTTGGATGTA AAATCTTTGC TGCTAGAGGC TTATGTTAAA
    GGACAGCAAG AGTTGCTCTA TGTAGTGCCC TTTGTTGCCA AAGTCTTAGA ATCTAGCATT
    CGTAGTGTGG TTTTTAGGCC ACCCAACCCC TGGACAATGG CTATTATGAA TGTATTAGCT
    GAGCTACATC AAGAACATGA CTTAAAGTTA AACTTGAAGT TTGAAATTGA GGTCCTCTGT
    AAGAATCTTG CATTAGACAT CAATGAGCTA AAACCTGGAA ACCTCCTAAA GGATAAAGAT
    CGCCTGAAGA ACTTAGATGA GCAACTCTCT GCTCCAAAGA AAGATGTCAA GCAGCCAGAG
    GAGCTCCCTC CTATCACAAC CACAACAACT TCTACAACAC CAGCTACCAG CACCACCTGT
    ACAGCCACTG TTCCACCACA GCCACAGTAC AGTTACCATG ACATCAATGT CTATTCCCTC
    GCAGGCCTGG CACCACACAT TACTCTAAAC CCAACAATTC CCTTGTTTCA GGCCCATCCA
    CAGCTGAAGC AGTGTGTGCG CCAAGCAATC GAACGGGCTG TCCAGGAGCT GGTCCATCCT
    GTGGTGGATC GATCAATTAA GATTGCCATG ACTACTTGTG AGCAAATAGT CAGGAAAGAT
    TTTGCCCTGG ATTCTGAGGA ATCTCGAATG CGAATAGCAG CTCATCACAT GATGCGGAAC
    TTGACAGCTG GAATGGCTAT GATTACTTGC AGGGAACCTT TACTCATGAG CATATCAACC
    AACCTGAAAA ACAGTTTTGC CTCAGCCCTT CGTACTGCAA GCCCACAACA AAGGGAAATG
    ATGGATCAGG CAGCTGCTCA ATTAGCTCAG GACAATTGTG AATTAGCTTG CTGTTTTATT
    CAGAAGACTG CAGTAGAAAA AGCAGGCCCT GAGATGGACA AGAGATTAGC AACTGAATTT
    GAGCTGAGAA AACATGCTAG GCAAGAAGGG CGAAGGTATT GTGATCCTGT TGTGTTAACA
    TACCAAGCTG AGCGGATGCC AGAGCAAATC AGGCTGAAAG TTGGTGGTGT GGACCCAAAG
    CAGCTGGCTG TTTATGAAGA GTTTGCACGC AATGTGCCTG GCTTCTTGCC TACAAATGAT
    TTAAGTCAGC CCACAGGATT TTTAGCCCAG CCCATGAAGC AAGCTTGGGC AACAGATGAT
    GTAGCTCAGA TTTATGATAA GTGTATTACA GAACTGGAGC AGCATCTTCA CGCTATCCCG
    CCAACTTTGG CCATGAACCC CCAAGCTCAG GCCCTCAGAA GTCTTCTGGA GGTTGTAGTA
    TTATCACGGA ACTCTCGAGA TGCCATTGCT GCTCTTGGAT TGCTCCAGAA GGCTGTAGAG
    GGCTTACTGG ATGCCACCAG TGGTGCTGAT GCTGACCTCC TTCTTCGCTA CAGAGAGTGC
    CACCTCTTGG TCTTGAAAGC TCTTCAAGAT GGCCGGGCAT ATGGGTCTCC ATGGTGTAAC
    AAACAGATCA CAAGGTGCCT AATTGAGTGT CGGGATGAAT ACAAATATAA TGTGGAGGCA
    GTGGAGTTGC TAATTCGCAA TCATTTGGTC AACATGCAAC AGTATGATCT TCACCTTGCT
    CAGTCAATGG AGAATGGTTT AAACTACATG GCTGTGGCAT TTGCTATGCA ATTGGTAAAA
    ATACTGCTGG TGGATGAAAG GAGTGTTGCC CATGTTACTG AGGCAGATCT GTTCCACACC
    ATTGAAACCC TCATGAGGAT TAATGCTCAC TCCAGAGGCA ATGCTCCTGA AGGATTGCCC
    CAACTGATGG AAGTAGTGCG ATCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGC
    CCTAACTTTA TGATGCATTC TGGGATCTCT CAAGCCTCAG AGTATGATGA TCCTCCAGGC
    CTGAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCAGCA
    GCTGGTCGTG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGGTAGA GCTTTTGGAA
    AGAAAGATGC ACCAGCAAGG AATCCTGAAG ACTGATGACC TCATAACAAG GTTCTTTCGT
    CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCAGAGCA GCAGCACAAT
    CCTGCTGCTA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTC
    CGACTCATTG CACTGCTAGT AAAGCACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT
    AATCTGCTGA ATAAGGTCCT CGGTATAGTA GTGGGAGTTC TCCTACAGGA TCATGATGTT
    CGGCAAAGTG AATTTCAGCA ACTTCCCTAC CACCGAATTT TTATCATGCT GCTCTTGGAA
    CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC
    AACACATTCC ACATTTTGAG GCCTACCAAA GCTCCTGGCT TTGTGTATGC TTGGCTTGAA
    CTGATCTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ACACGCCACA GCAGAAGGGA
    TGGCCTATGT ATGCACAGTT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA
    AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG GGTGCTGCTG
    GTTCTTTTGC ATGATTTTCC TGAGTTCCTT TGTGATTACC ATTATGGATT TTGTGACGTG
    ATCCCACCTA ATTGTATCCA GCTAAGAAAT CTGATCCTGA GTGCCTTCCC AAGAAACATG
    AGGCTCCCAG ACCCATTCAC TCCTAATCTG AAGGTGGATA TGTTGAGTGA AATTAACATC
    GCTCCCCGGA TTCTCACCAA TTTTACTGGA GTGATGCCAC CTCAATTCAA AAAGGATTTG
    GATTCCTATC TTAAAACTCG ATCACCTGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA
    CAGGTATCCA ATGAGCCTGG TAATCGCTAC AACCTCCAGC TCATTAATGC ACTGGTGCTC
    TATGTAGGGA CTCAGGCCAT TGCACACATC CACAACAAGG GCAGCACACC TTCAATGAGC
    ACCATCACGC ACTCAGCTCA CATGGACATC TTCCAGAACC TGGCTGTGGA TTTGGACACT
    GAAGGTCGGT ATCTCTTCTT GAATGCAATT GCAAATCAGC TGCGGTACCC AAATAGCCAC
    ACTCACTACT TCAGCTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAACAC TGAAGCTATT
    CAAGAACAGA TCACAAGGGT TCTTTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG
    GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAATCCAG CATTTAAATT CTGGAACCAT
    GAATTTGTAC ACTGTGCCCC AGAAATTGAA AAGTTATTCC AGTCCGTCGC ACAGTGCTGC
    ATGGGACAAA AGCAGGCTCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834135.1
    CDS328..7518
    Translation

    Target ORF information:

    RefSeq Version XM_024978367.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGAGTAAGAT GAAACCTTCG
    GACTTGTCTC AGGTGTGGCC AGAGGCAAAT CAGCACTTTA GTAAAGAGAT AGATGATGAA
    GCAAACAGCT ATTTCCAGAG AATATATAAT CACCCACCAC ATCCAACCAT GTCTGTTGAT
    GAGGTATTAG AAATGCTACA GAGATTTAAA GATTCTACCA TAAAGAGGGA ACGAGAAGTA
    TTTAACTGTA TGCTAAGGAA CTTGTTCGAA GAATATCGGT TCTTCCCCCA ATACCCTGAT
    AAAGAGTTAC ATATAACAGC CTGCCTGTTT GGTGGAATAA TCGAAAAAGG ACTGGTCACT
    TATATGGCAC TAGGTTTGGC CCTGCGATAC GTTCTTGAAG CCTTACGCAA GCCTTTTGGA
    TCCAAAATGT ATTATTTTGG GATTGCTGCA CTAGATAGAT TTAAAAATAG ATTGAAGGAC
    TATCCCCAGT ATTGTCAGCA CTTGGCTTCG ATCAGTCACT TTATGCAATT TCCACATCAT
    TTACAGGAGT ATATTGAGTA TGGACAGCAA TCTAGAGATC CTCCTGTGAA AATGCAGGGC
    TCTATCACAA CCCCTGGAAG TATTGCACTG GCTCAAGCCC AGGCCCAGGC CCAGGTTCCA
    GCAAAAGCTC CCCTTGCTGG CCAGGTTAGC ACTATGGTAA CCACCTCAAC AACCACCACT
    GTTGCTAAAA CGGTTACAGT CACCAGGCCA ACTGGAGTCA GCTTTAAGAA AGATGTGCCA
    CCTTCTATTA ATACAACAAA TATAGATACA TTGCTTGTGG CCACAGATCA AACTGAGAGA
    ATCGTAGAGC CCCCAGAAAA TATCCAGGAG AAAATTGCTT TTATCTTCAA CAACCTTTCC
    CAGTCAAATA TGACACAAAA GGTTGAAGAA CTAAAGGAAA CTGTGAAGGA AGAATTTATG
    CCTTGGGTTT CACAGTACCT GGTTATGAAG AGAGTCAGTA TTGAGCCAAA CTTTCACAGC
    CTGTATTCTA ACTTCCTTGA CACGCTGAAG AATCCTGAGT TTAATAAGAT GGTTCTGAAT
    GAGACCTATA GAAACATCAA AGTACTCCTG ACCTCTGATA AAGCTGCAGC CAATTTCTCT
    GATCGGTCTT TGCTGAAGAA CTTGGGACAT TGGCTAGGAA TGATTACACT AGCTAAAAAT
    AAACCCATCT TACACACTGA CTTGGATGTA AAATCTTTGC TGCTAGAGGC TTATGTTAAA
    GGACAGCAAG AGTTGCTCTA TGTAGTGCCC TTTGTTGCCA AAGTCTTAGA ATCTAGCATT
    CGTAGTGTGG TTTTTAGGCC ACCCAACCCC TGGACAATGG CTATTATGAA TGTATTAGCT
    GAGCTACATC AAGAACATGA CTTAAAGTTA AACTTGAAGT TTGAAATTGA GGTCCTCTGT
    AAGAATCTTG CATTAGACAT CAATGAGCTA AAACCTGGAA ACCTCCTAAA GGATAAAGAT
    CGCCTGAAGA ACTTAGATGA GCAACTCTCT GCTCCAAAGA AAGATGTCAA GCAGCCAGAG
    GAGCTCCCTC CTATCACAAC CACAACAACT TCTACAACAC CAGCTACCAG CACCACCTGT
    ACAGCCACTG TTCCACCACA GCCACAGTAC AGTTACCATG ACATCAATGT CTATTCCCTC
    GCAGGCCTGG CACCACACAT TACTCTAAAC CCAACAATTC CCTTGTTTCA GGCCCATCCA
    CAGCTGAAGC AGTGTGTGCG CCAAGCAATC GAACGGGCTG TCCAGGAGCT GGTCCATCCT
    GTGGTGGATC GATCAATTAA GATTGCCATG ACTACTTGTG AGCAAATAGT CAGGAAAGAT
    TTTGCCCTGG ATTCTGAGGA ATCTCGAATG CGAATAGCAG CTCATCACAT GATGCGGAAC
    TTGACAGCTG GAATGGCTAT GATTACTTGC AGGGAACCTT TACTCATGAG CATATCAACC
    AACCTGAAAA ACAGTTTTGC CTCAGCCCTT CGTACTGCAA GCCCACAACA AAGGGAAATG
    ATGGATCAGG CAGCTGCTCA ATTAGCTCAG GACAATTGTG AATTAGCTTG CTGTTTTATT
    CAGAAGACTG CAGTAGAAAA AGCAGGCCCT GAGATGGACA AGAGATTAGC AACTGAATTT
    GAGCTGAGAA AACATGCTAG GCAAGAAGGG CGAAGGTATT GTGATCCTGT TGTGTTAACA
    TACCAAGCTG AGCGGATGCC AGAGCAAATC AGGCTGAAAG TTGGTGGTGT GGACCCAAAG
    CAGCTGGCTG TTTATGAAGA GTTTGCACGC AATGTGCCTG GCTTCTTGCC TACAAATGAT
    TTAAGTCAGC CCACAGGATT TTTAGCCCAG CCCATGAAGC AAGCTTGGGC AACAGATGAT
    GTAGCTCAGA TTTATGATAA GTGTATTACA GAACTGGAGC AGCATCTTCA CGCTATCCCG
    CCAACTTTGG CCATGAACCC CCAAGCTCAG GCCCTCAGAA GTCTTCTGGA GGTTGTAGTA
    TTATCACGGA ACTCTCGAGA TGCCATTGCT GCTCTTGGAT TGCTCCAGAA GGCTGTAGAG
    GGCTTACTGG ATGCCACCAG TGGTGCTGAT GCTGACCTCC TTCTTCGCTA CAGAGAGTGC
    CACCTCTTGG TCTTGAAAGC TCTTCAAGAT GGCCGGGCAT ATGGGTCTCC ATGGTGTAAC
    AAACAGATCA CAAGGTGCCT AATTGAGTGT CGGGATGAAT ACAAATATAA TGTGGAGGCA
    GTGGAGTTGC TAATTCGCAA TCATTTGGTC AACATGCAAC AGTATGATCT TCACCTTGCT
    CAGTCAATGG AGAATGGTTT AAACTACATG GCTGTGGCAT TTGCTATGCA ATTGGTAAAA
    ATACTGCTGG TGGATGAAAG GAGTGTTGCC CATGTTACTG AGGCAGATCT GTTCCACACC
    ATTGAAACCC TCATGAGGAT TAATGCTCAC TCCAGAGGCA ATGCTCCTGA AGGATTGCCC
    CAACTGATGG AAGTAGTGCG ATCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGC
    CCTAACTTTA TGATGCATTC TGGGATCTCT CAAGCCTCAG AGTATGATGA TCCTCCAGGC
    CTGAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCAGCA
    GCTGGTCGTG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGGTAGA GCTTTTGGAA
    AGAAAGATGC ACCAGCAAGG AATCCTGAAG ACTGATGACC TCATAACAAG GTTCTTTCGT
    CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCAGAGCA GCAGCACAAT
    CCTGCTGCTA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTC
    CGACTCATTG CACTGCTAGT AAAGCACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT
    AATCTGCTGA ATAAGGTCCT CGGTATAGTA GTGGGAGTTC TCCTACAGGA TCATGATGTT
    CGGCAAAGTG AATTTCAGCA ACTTCCCTAC CACCGAATTT TTATCATGCT GCTCTTGGAA
    CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC
    AACACATTCC ACATTTTGAG GCCTACCAAA GCTCCTGGCT TTGTGTATGC TTGGCTTGAA
    CTGATCTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ACACGCCACA GCAGAAGGGA
    TGGCCTATGT ATGCACAGTT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA
    AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG GGTGCTGCTG
    GTTCTTTTGC ATGATTTTCC TGAGTTCCTT TGTGATTACC ATTATGGATT TTGTGACGTG
    ATCCCACCTA ATTGTATCCA GCTAAGAAAT CTGATCCTGA GTGCCTTCCC AAGAAACATG
    AGGCTCCCAG ACCCATTCAC TCCTAATCTG AAGGTGGATA TGTTGAGTGA AATTAACATC
    GCTCCCCGGA TTCTCACCAA TTTTACTGGA GTGATGCCAC CTCAATTCAA AAAGGATTTG
    GATTCCTATC TTAAAACTCG ATCACCTGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA
    CAGGTATCCA ATGAGCCTGG TAATCGCTAC AACCTCCAGC TCATTAATGC ACTGGTGCTC
    TATGTAGGGA CTCAGGCCAT TGCACACATC CACAACAAGG GCAGCACACC TTCAATGAGC
    ACCATCACGC ACTCAGCTCA CATGGACATC TTCCAGAACC TGGCTGTGGA TTTGGACACT
    GAAGGTCGGT ATCTCTTCTT GAATGCAATT GCAAATCAGC TGCGGTACCC AAATAGCCAC
    ACTCACTACT TCAGCTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAACAC TGAAGCTATT
    CAAGAACAGA TCACAAGGGT TCTTTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG
    GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAATCCAG CATTTAAATT CTGGAACCAT
    GAATTTGTAC ACTGTGCCCC AGAAATTGAA AAGTTATTCC AGTCCGTCGC ACAGTGCTGC
    ATGGGACAAA AGCAGGCTCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225088
    Clone ID Related Accession (Same CDS sequence) XM_024978368.1
    Accession Version XM_024978368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GGTGGTCTTG GCACAGGCAG CCTGACTGGT
    ATAGGAACTG GGGCTCTTGG ACTCCCTGCA GTGAATAGCG ATCCTTTTGT ACAGAGGAAA
    CTGGGCACCT CTGGACTGAA CCAGCCTACA TTCCAGCAGA GTAAGATGAA ACCTTCGGAC
    TTGTCTCAGG TGTGGCCAGA GGCAAATCAG CACTTTAGTA AAGAGATAGA TGATGAAGCA
    AACAGCTATT TCCAGAGAAT ATATAATCAC CCACCACATC CAACCATGTC TGTTGATGAG
    GTATTAGAAA TGCTACAGAG ATTTAAAGAT TCTACCATAA AGAGGGAACG AGAAGTATTT
    AACTGTATGC TAAGGAACTT GTTCGAAGAA TATCGGTTCT TCCCCCAATA CCCTGATAAA
    GAGTTACATA TAACAGCCTG CCTGTTTGGT GGAATAATCG AAAAAGGACT GGTCACTTAT
    ATGGCACTAG GTTTGGCCCT GCGATACGTT CTTGAAGCCT TACGCAAGCC TTTTGGATCC
    AAAATGTATT ATTTTGGGAT TGCTGCACTA GATAGATTTA AAAATAGATT GAAGGACTAT
    CCCCAGTATT GTCAGCACTT GGCTTCGATC AGTCACTTTA TGCAATTTCC ACATCATTTA
    CAGGAGTATA TTGAGTATGG ACAGCAATCT AGAGATCCTC CTGTGAAAAT GCAGGGCTCT
    ATCACAACCC CTGGAAGTAT TGCACTGGCT CAAGCCCAGG CCCAGGCCCA GGTTCCAGCA
    AAAGCTCCCC TTGCTGGCCA GGTTAGCACT ATGGTAACCA CCTCAACAAC CACCACTGTT
    GCTAAAACGG TTACAGTCAC CAGGCCAACT GGAGTCAGCT TTAAGAAAGA TGTGCCACCT
    TCTATTAATA CAACAAATAT AGATACATTG CTTGTGGCCA CAGATCAAAC TGAGAGAATC
    GTAGAGCCCC CAGAAAATAT CCAGGAGAAA ATTGCTTTTA TCTTCAACAA CCTTTCCCAG
    TCAAATATGA CACAAAAGGT TGAAGAACTA AAGGAAACTG TGAAGGAAGA ATTTATGCCT
    TGGGTTTCAC AGTACCTGGT TATGAAGAGA GTCAGTATTG AGCCAAACTT TCACAGCCTG
    TATTCTAACT TCCTTGACAC GCTGAAGAAT CCTGAGTTTA ATAAGATGGT TCTGAATGAG
    ACCTATAGAA ACATCAAAGT ACTCCTGACC TCTGATAAAG CTGCAGCCAA TTTCTCTGAT
    CGGTCTTTGC TGAAGAACTT GGGACATTGG CTAGGAATGA TTACACTAGC TAAAAATAAA
    CCCATCTTAC ACACTGACTT GGATGTAAAA TCTTTGCTGC TAGAGGCTTA TGTTAAAGGA
    CAGCAAGAGT TGCTCTATGT AGTGCCCTTT GTTGCCAAAG TCTTAGAATC TAGCATTCGT
    AGTGTGGTTT TTAGGCCACC CAACCCCTGG ACAATGGCTA TTATGAATGT ATTAGCTGAG
    CTACATCAAG AACATGACTT AAAGTTAAAC TTGAAGTTTG AAATTGAGGT CCTCTGTAAG
    AATCTTGCAT TAGACATCAA TGAGCTAAAA CCTGGAAACC TCCTAAAGGA TAAAGATCGC
    CTGAAGAACT TAGATGAGCA ACTCTCTGCT CCAAAGAAAG ATGTCAAGCA GCCAGAGGAG
    CTCCCTCCTA TCACAACCAC AACAACTTCT ACAACACCAG CTACCAGCAC CACCTGTACA
    GCCACTGTTC CACCACAGCC ACAGTACAGT TACCATGACA TCAATGTCTA TTCCCTCGCA
    GGCCTGGCAC CACACATTAC TCTAAACCCA ACAATTCCCT TGTTTCAGGC CCATCCACAG
    CTGAAGCAGT GTGTGCGCCA AGCAATCGAA CGGGCTGTCC AGGAGCTGGT CCATCCTGTG
    GTGGATCGAT CAATTAAGAT TGCCATGACT ACTTGTGAGC AAATAGTCAG GAAAGATTTT
    GCCCTGGATT CTGAGGAATC TCGAATGCGA ATAGCAGCTC ATCACATGAT GCGGAACTTG
    ACAGCTGGAA TGGCTATGAT TACTTGCAGG GAACCTTTAC TCATGAGCAT ATCAACCAAC
    CTGAAAAACA GTTTTGCCTC AGCCCTTCGT ACTGCAAGCC CACAACAAAG GGAAATGATG
    GATCAGGCAG CTGCTCAATT AGCTCAGGAC AATTGTGAAT TAGCTTGCTG TTTTATTCAG
    AAGACTGCAG TAGAAAAAGC AGGCCCTGAG ATGGACAAGA GATTAGCAAC TGAATTTGAG
    CTGAGAAAAC ATGCTAGGCA AGAAGGGCGA AGGTATTGTG ATCCTGTTGT GTTAACATAC
    CAAGCTGAGC GGATGCCAGA GCAAATCAGG CTGAAAGTTG GTGGTGTGGA CCCAAAGCAG
    CTGGCTGTTT ATGAAGAGTT TGCACGCAAT GTGCCTGGCT TCTTGCCTAC AAATGATTTA
    AGTCAGCCCA CAGGATTTTT AGCCCAGCCC ATGAAGCAAG CTTGGGCAAC AGATGATGTA
    GCTCAGATTT ATGATAAGTG TATTACAGAA CTGGAGCAGC ATCTTCACGC TATCCCGCCA
    ACTTTGGCCA TGAACCCCCA AGCTCAGGCC CTCAGAAGTC TTCTGGAGGT TGTAGTATTA
    TCACGGAACT CTCGAGATGC CATTGCTGCT CTTGGATTGC TCCAGAAGGC TGTAGAGGGC
    TTACTGGATG CCACCAGTGG TGCTGATGCT GACCTCCTTC TTCGCTACAG AGAGTGCCAC
    CTCTTGGTCT TGAAAGCTCT TCAAGATGGC CGGGCATATG GGTCTCCATG GTGTAACAAA
    CAGATCACAA GGTGCCTAAT TGAGTGTCGG GATGAATACA AATATAATGT GGAGGCAGTG
    GAGTTGCTAA TTCGCAATCA TTTGGTCAAC ATGCAACAGT ATGATCTTCA CCTTGCTCAG
    TCAATGGAGA ATGGTTTAAA CTACATGGCT GTGGCATTTG CTATGCAATT GGTAAAAATA
    CTGCTGGTGG ATGAAAGGAG TGTTGCCCAT GTTACTGAGG CAGATCTGTT CCACACCATT
    GAAACCCTCA TGAGGATTAA TGCTCACTCC AGAGGCAATG CTCCTGAAGG ATTGCCCCAA
    CTGATGGAAG TAGTGCGATC CAACTATGAA GCAATGATTG ATCGTGCTCA TGGAGGCCCT
    AACTTTATGA TGCATTCTGG GATCTCTCAA GCCTCAGAGT ATGATGATCC TCCAGGCCTG
    AGGGAGAAGG CAGAGTATCT TCTGAGGGAA TGGGTGAATC TCTACCATTC AGCAGCAGCT
    GGTCGTGACA GTACCAAAGC TTTCTCTGCG TTTGTTGGAC AGGTAGAGCT TTTGGAAAGA
    AAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834136.1
    CDS328..7515
    Translation

    Target ORF information:

    RefSeq Version XM_024978368.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GGTGGTCTTG GCACAGGCAG CCTGACTGGT
    ATAGGAACTG GGGCTCTTGG ACTCCCTGCA GTGAATAGCG ATCCTTTTGT ACAGAGGAAA
    CTGGGCACCT CTGGACTGAA CCAGCCTACA TTCCAGCAGA GTAAGATGAA ACCTTCGGAC
    TTGTCTCAGG TGTGGCCAGA GGCAAATCAG CACTTTAGTA AAGAGATAGA TGATGAAGCA
    AACAGCTATT TCCAGAGAAT ATATAATCAC CCACCACATC CAACCATGTC TGTTGATGAG
    GTATTAGAAA TGCTACAGAG ATTTAAAGAT TCTACCATAA AGAGGGAACG AGAAGTATTT
    AACTGTATGC TAAGGAACTT GTTCGAAGAA TATCGGTTCT TCCCCCAATA CCCTGATAAA
    GAGTTACATA TAACAGCCTG CCTGTTTGGT GGAATAATCG AAAAAGGACT GGTCACTTAT
    ATGGCACTAG GTTTGGCCCT GCGATACGTT CTTGAAGCCT TACGCAAGCC TTTTGGATCC
    AAAATGTATT ATTTTGGGAT TGCTGCACTA GATAGATTTA AAAATAGATT GAAGGACTAT
    CCCCAGTATT GTCAGCACTT GGCTTCGATC AGTCACTTTA TGCAATTTCC ACATCATTTA
    CAGGAGTATA TTGAGTATGG ACAGCAATCT AGAGATCCTC CTGTGAAAAT GCAGGGCTCT
    ATCACAACCC CTGGAAGTAT TGCACTGGCT CAAGCCCAGG CCCAGGCCCA GGTTCCAGCA
    AAAGCTCCCC TTGCTGGCCA GGTTAGCACT ATGGTAACCA CCTCAACAAC CACCACTGTT
    GCTAAAACGG TTACAGTCAC CAGGCCAACT GGAGTCAGCT TTAAGAAAGA TGTGCCACCT
    TCTATTAATA CAACAAATAT AGATACATTG CTTGTGGCCA CAGATCAAAC TGAGAGAATC
    GTAGAGCCCC CAGAAAATAT CCAGGAGAAA ATTGCTTTTA TCTTCAACAA CCTTTCCCAG
    TCAAATATGA CACAAAAGGT TGAAGAACTA AAGGAAACTG TGAAGGAAGA ATTTATGCCT
    TGGGTTTCAC AGTACCTGGT TATGAAGAGA GTCAGTATTG AGCCAAACTT TCACAGCCTG
    TATTCTAACT TCCTTGACAC GCTGAAGAAT CCTGAGTTTA ATAAGATGGT TCTGAATGAG
    ACCTATAGAA ACATCAAAGT ACTCCTGACC TCTGATAAAG CTGCAGCCAA TTTCTCTGAT
    CGGTCTTTGC TGAAGAACTT GGGACATTGG CTAGGAATGA TTACACTAGC TAAAAATAAA
    CCCATCTTAC ACACTGACTT GGATGTAAAA TCTTTGCTGC TAGAGGCTTA TGTTAAAGGA
    CAGCAAGAGT TGCTCTATGT AGTGCCCTTT GTTGCCAAAG TCTTAGAATC TAGCATTCGT
    AGTGTGGTTT TTAGGCCACC CAACCCCTGG ACAATGGCTA TTATGAATGT ATTAGCTGAG
    CTACATCAAG AACATGACTT AAAGTTAAAC TTGAAGTTTG AAATTGAGGT CCTCTGTAAG
    AATCTTGCAT TAGACATCAA TGAGCTAAAA CCTGGAAACC TCCTAAAGGA TAAAGATCGC
    CTGAAGAACT TAGATGAGCA ACTCTCTGCT CCAAAGAAAG ATGTCAAGCA GCCAGAGGAG
    CTCCCTCCTA TCACAACCAC AACAACTTCT ACAACACCAG CTACCAGCAC CACCTGTACA
    GCCACTGTTC CACCACAGCC ACAGTACAGT TACCATGACA TCAATGTCTA TTCCCTCGCA
    GGCCTGGCAC CACACATTAC TCTAAACCCA ACAATTCCCT TGTTTCAGGC CCATCCACAG
    CTGAAGCAGT GTGTGCGCCA AGCAATCGAA CGGGCTGTCC AGGAGCTGGT CCATCCTGTG
    GTGGATCGAT CAATTAAGAT TGCCATGACT ACTTGTGAGC AAATAGTCAG GAAAGATTTT
    GCCCTGGATT CTGAGGAATC TCGAATGCGA ATAGCAGCTC ATCACATGAT GCGGAACTTG
    ACAGCTGGAA TGGCTATGAT TACTTGCAGG GAACCTTTAC TCATGAGCAT ATCAACCAAC
    CTGAAAAACA GTTTTGCCTC AGCCCTTCGT ACTGCAAGCC CACAACAAAG GGAAATGATG
    GATCAGGCAG CTGCTCAATT AGCTCAGGAC AATTGTGAAT TAGCTTGCTG TTTTATTCAG
    AAGACTGCAG TAGAAAAAGC AGGCCCTGAG ATGGACAAGA GATTAGCAAC TGAATTTGAG
    CTGAGAAAAC ATGCTAGGCA AGAAGGGCGA AGGTATTGTG ATCCTGTTGT GTTAACATAC
    CAAGCTGAGC GGATGCCAGA GCAAATCAGG CTGAAAGTTG GTGGTGTGGA CCCAAAGCAG
    CTGGCTGTTT ATGAAGAGTT TGCACGCAAT GTGCCTGGCT TCTTGCCTAC AAATGATTTA
    AGTCAGCCCA CAGGATTTTT AGCCCAGCCC ATGAAGCAAG CTTGGGCAAC AGATGATGTA
    GCTCAGATTT ATGATAAGTG TATTACAGAA CTGGAGCAGC ATCTTCACGC TATCCCGCCA
    ACTTTGGCCA TGAACCCCCA AGCTCAGGCC CTCAGAAGTC TTCTGGAGGT TGTAGTATTA
    TCACGGAACT CTCGAGATGC CATTGCTGCT CTTGGATTGC TCCAGAAGGC TGTAGAGGGC
    TTACTGGATG CCACCAGTGG TGCTGATGCT GACCTCCTTC TTCGCTACAG AGAGTGCCAC
    CTCTTGGTCT TGAAAGCTCT TCAAGATGGC CGGGCATATG GGTCTCCATG GTGTAACAAA
    CAGATCACAA GGTGCCTAAT TGAGTGTCGG GATGAATACA AATATAATGT GGAGGCAGTG
    GAGTTGCTAA TTCGCAATCA TTTGGTCAAC ATGCAACAGT ATGATCTTCA CCTTGCTCAG
    TCAATGGAGA ATGGTTTAAA CTACATGGCT GTGGCATTTG CTATGCAATT GGTAAAAATA
    CTGCTGGTGG ATGAAAGGAG TGTTGCCCAT GTTACTGAGG CAGATCTGTT CCACACCATT
    GAAACCCTCA TGAGGATTAA TGCTCACTCC AGAGGCAATG CTCCTGAAGG ATTGCCCCAA
    CTGATGGAAG TAGTGCGATC CAACTATGAA GCAATGATTG ATCGTGCTCA TGGAGGCCCT
    AACTTTATGA TGCATTCTGG GATCTCTCAA GCCTCAGAGT ATGATGATCC TCCAGGCCTG
    AGGGAGAAGG CAGAGTATCT TCTGAGGGAA TGGGTGAATC TCTACCATTC AGCAGCAGCT
    GGTCGTGACA GTACCAAAGC TTTCTCTGCG TTTGTTGGAC AGGTAGAGCT TTTGGAAAGA
    AAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225089
    Clone ID Related Accession (Same CDS sequence) XM_024978369.1
    Accession Version XM_024978369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGACGGACTT GTCTCAGGTG
    TGGCCAGAGG CAAATCAGCA CTTTAGTAAA GAGATAGATG ATGAAGCAAA CAGCTATTTC
    CAGAGAATAT ATAATCACCC ACCACATCCA ACCATGTCTG TTGATGAGGT ATTAGAAATG
    CTACAGAGAT TTAAAGATTC TACCATAAAG AGGGAACGAG AAGTATTTAA CTGTATGCTA
    AGGAACTTGT TCGAAGAATA TCGGTTCTTC CCCCAATACC CTGATAAAGA GTTACATATA
    ACAGCCTGCC TGTTTGGTGG AATAATCGAA AAAGGACTGG TCACTTATAT GGCACTAGGT
    TTGGCCCTGC GATACGTTCT TGAAGCCTTA CGCAAGCCTT TTGGATCCAA AATGTATTAT
    TTTGGGATTG CTGCACTAGA TAGATTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT
    CAGCACTTGG CTTCGATCAG TCACTTTATG CAATTTCCAC ATCATTTACA GGAGTATATT
    GAGTATGGAC AGCAATCTAG AGATCCTCCT GTGAAAATGC AGGGCTCTAT CACAACCCCT
    GGAAGTATTG CACTGGCTCA AGCCCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCCCTT
    GCTGGCCAGG TTAGCACTAT GGTAACCACC TCAACAACCA CCACTGTTGC TAAAACGGTT
    ACAGTCACCA GGCCAACTGG AGTCAGCTTT AAGAAAGATG TGCCACCTTC TATTAATACA
    ACAAATATAG ATACATTGCT TGTGGCCACA GATCAAACTG AGAGAATCGT AGAGCCCCCA
    GAAAATATCC AGGAGAAAAT TGCTTTTATC TTCAACAACC TTTCCCAGTC AAATATGACA
    CAAAAGGTTG AAGAACTAAA GGAAACTGTG AAGGAAGAAT TTATGCCTTG GGTTTCACAG
    TACCTGGTTA TGAAGAGAGT CAGTATTGAG CCAAACTTTC ACAGCCTGTA TTCTAACTTC
    CTTGACACGC TGAAGAATCC TGAGTTTAAT AAGATGGTTC TGAATGAGAC CTATAGAAAC
    ATCAAAGTAC TCCTGACCTC TGATAAAGCT GCAGCCAATT TCTCTGATCG GTCTTTGCTG
    AAGAACTTGG GACATTGGCT AGGAATGATT ACACTAGCTA AAAATAAACC CATCTTACAC
    ACTGACTTGG ATGTAAAATC TTTGCTGCTA GAGGCTTATG TTAAAGGACA GCAAGAGTTG
    CTCTATGTAG TGCCCTTTGT TGCCAAAGTC TTAGAATCTA GCATTCGTAG TGTGGTTTTT
    AGGCCACCCA ACCCCTGGAC AATGGCTATT ATGAATGTAT TAGCTGAGCT ACATCAAGAA
    CATGACTTAA AGTTAAACTT GAAGTTTGAA ATTGAGGTCC TCTGTAAGAA TCTTGCATTA
    GACATCAATG AGCTAAAACC TGGAAACCTC CTAAAGGATA AAGATCGCCT GAAGAACTTA
    GATGAGCAAC TCTCTGCTCC AAAGAAAGAT GTCAAGCAGC CAGAGGAGCT CCCTCCTATC
    ACAACCACAA CAACTTCTAC AACACCAGCT ACCAGCACCA CCTGTACAGC CACTGTTCCA
    CCACAGCCAC AGTACAGTTA CCATGACATC AATGTCTATT CCCTCGCAGG CCTGGCACCA
    CACATTACTC TAAACCCAAC AATTCCCTTG TTTCAGGCCC ATCCACAGCT GAAGCAGTGT
    GTGCGCCAAG CAATCGAACG GGCTGTCCAG GAGCTGGTCC ATCCTGTGGT GGATCGATCA
    ATTAAGATTG CCATGACTAC TTGTGAGCAA ATAGTCAGGA AAGATTTTGC CCTGGATTCT
    GAGGAATCTC GAATGCGAAT AGCAGCTCAT CACATGATGC GGAACTTGAC AGCTGGAATG
    GCTATGATTA CTTGCAGGGA ACCTTTACTC ATGAGCATAT CAACCAACCT GAAAAACAGT
    TTTGCCTCAG CCCTTCGTAC TGCAAGCCCA CAACAAAGGG AAATGATGGA TCAGGCAGCT
    GCTCAATTAG CTCAGGACAA TTGTGAATTA GCTTGCTGTT TTATTCAGAA GACTGCAGTA
    GAAAAAGCAG GCCCTGAGAT GGACAAGAGA TTAGCAACTG AATTTGAGCT GAGAAAACAT
    GCTAGGCAAG AAGGGCGAAG GTATTGTGAT CCTGTTGTGT TAACATACCA AGCTGAGCGG
    ATGCCAGAGC AAATCAGGCT GAAAGTTGGT GGTGTGGACC CAAAGCAGCT GGCTGTTTAT
    GAAGAGTTTG CACGCAATGT GCCTGGCTTC TTGCCTACAA ATGATTTAAG TCAGCCCACA
    GGATTTTTAG CCCAGCCCAT GAAGCAAGCT TGGGCAACAG ATGATGTAGC TCAGATTTAT
    GATAAGTGTA TTACAGAACT GGAGCAGCAT CTTCACGCTA TCCCGCCAAC TTTGGCCATG
    AACCCCCAAG CTCAGGCCCT CAGAAGTCTT CTGGAGGTTG TAGTATTATC ACGGAACTCT
    CGAGATGCCA TTGCTGCTCT TGGATTGCTC CAGAAGGCTG TAGAGGGCTT ACTGGATGCC
    ACCAGTGGTG CTGATGCTGA CCTCCTTCTT CGCTACAGAG AGTGCCACCT CTTGGTCTTG
    AAAGCTCTTC AAGATGGCCG GGCATATGGG TCTCCATGGT GTAACAAACA GATCACAAGG
    TGCCTAATTG AGTGTCGGGA TGAATACAAA TATAATGTGG AGGCAGTGGA GTTGCTAATT
    CGCAATCATT TGGTCAACAT GCAACAGTAT GATCTTCACC TTGCTCAGTC AATGGAGAAT
    GGTTTAAACT ACATGGCTGT GGCATTTGCT ATGCAATTGG TAAAAATACT GCTGGTGGAT
    GAAAGGAGTG TTGCCCATGT TACTGAGGCA GATCTGTTCC ACACCATTGA AACCCTCATG
    AGGATTAATG CTCACTCCAG AGGCAATGCT CCTGAAGGAT TGCCCCAACT GATGGAAGTA
    GTGCGATCCA ACTATGAAGC AATGATTGAT CGTGCTCATG GAGGCCCTAA CTTTATGATG
    CATTCTGGGA TCTCTCAAGC CTCAGAGTAT GATGATCCTC CAGGCCTGAG GGAGAAGGCA
    GAGTATCTTC TGAGGGAATG GGTGAATCTC TACCATTCAG CAGCAGCTGG TCGTGACAGT
    ACCAAAGCTT TCTCTGCGTT TGTTGGACAG GTAGAGCTTT TGGAAAGAAA GATGCACCAG
    CAAGGAATCC TGAAGACTGA TGACCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG
    TGTGTTGAAA TCAGTTACCG TGCTCAGGCA GAGCAGCAGC ACAATCCTGC TGCTAATCCC
    ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTCCGACT CATTGCACTG
    CTAGTAAAGC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAATAAG
    GTCCTCGGTA TAGTAGTGGG AGTTCTCCTA CAGGATCATG ATGTTCGGCA AAGTGAATTT
    CAGCAACTTC CCTACCACCG AATTTTTATC ATGCTGCTCT TGGAACTCAA TGCACCTGAG
    CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAACAC ATTCCACATT
    TTGAGGCCTA CCAAAGCTCC TGGCTTTGTG TATGCTTGGC TTGAACTGAT CTCCCATCGG
    ATATTTATTG CAAGAATGCT GGCACACACG CCACAGCAGA AGGGATGGCC TATGTATGCA
    CAGTTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC
    AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGGGTGC TGCTGGTTCT TTTGCATGAT
    TTTCCTGAGT TCCTTTGTGA TTACCATTAT GGATTTTGTG ACGTGATCCC ACCTAATTGT
    ATCCAGCTAA GAAATCTGAT CCTGAGTGCC TTCCCAAGAA ACATGAGGCT CCCAGACCCA
    TTCACTCCTA ATCTGAAGGT GGATATGTTG AGTGAAATTA ACATCGCTCC CCGGATTCTC
    ACCAATTTTA CTGGAGTGAT GCCACCTCAA TTCAAAAAGG ATTTGGATTC CTATCTTAAA
    ACTCGATCAC CTGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAG
    CCTGGTAATC GCTACAACCT CCAGCTCATT AATGCACTGG TGCTCTATGT AGGGACTCAG
    GCCATTGCAC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACGCACTCA
    GCTCACATGG ACATCTTCCA GAACCTGGCT GTGGATTTGG ACACTGAAGG TCGGTATCTC
    TTCTTGAATG CAATTGCAAA TCAGCTGCGG TACCCAAATA GCCACACTCA CTACTTCAGC
    TGCACCATGC TGTACCTTTT TGCAGAGGCC AACACTGAAG CTATTCAAGA ACAGATCACA
    AGGGTTCTTT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC
    TTCATTGAGC TGATTAAAAA TCCAGCATTT AAATTCTGGA ACCATGAATT TGTACACTGT
    GCCCCAGAAA TTGAAAAGTT ATTCCAGTCC GTCGCACAGT GCTGCATGGG ACAAAAGCAG
    GCTCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834137.1
    CDS328..7503
    Translation

    Target ORF information:

    RefSeq Version XM_024978369.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGACGGACTT GTCTCAGGTG
    TGGCCAGAGG CAAATCAGCA CTTTAGTAAA GAGATAGATG ATGAAGCAAA CAGCTATTTC
    CAGAGAATAT ATAATCACCC ACCACATCCA ACCATGTCTG TTGATGAGGT ATTAGAAATG
    CTACAGAGAT TTAAAGATTC TACCATAAAG AGGGAACGAG AAGTATTTAA CTGTATGCTA
    AGGAACTTGT TCGAAGAATA TCGGTTCTTC CCCCAATACC CTGATAAAGA GTTACATATA
    ACAGCCTGCC TGTTTGGTGG AATAATCGAA AAAGGACTGG TCACTTATAT GGCACTAGGT
    TTGGCCCTGC GATACGTTCT TGAAGCCTTA CGCAAGCCTT TTGGATCCAA AATGTATTAT
    TTTGGGATTG CTGCACTAGA TAGATTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT
    CAGCACTTGG CTTCGATCAG TCACTTTATG CAATTTCCAC ATCATTTACA GGAGTATATT
    GAGTATGGAC AGCAATCTAG AGATCCTCCT GTGAAAATGC AGGGCTCTAT CACAACCCCT
    GGAAGTATTG CACTGGCTCA AGCCCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCCCTT
    GCTGGCCAGG TTAGCACTAT GGTAACCACC TCAACAACCA CCACTGTTGC TAAAACGGTT
    ACAGTCACCA GGCCAACTGG AGTCAGCTTT AAGAAAGATG TGCCACCTTC TATTAATACA
    ACAAATATAG ATACATTGCT TGTGGCCACA GATCAAACTG AGAGAATCGT AGAGCCCCCA
    GAAAATATCC AGGAGAAAAT TGCTTTTATC TTCAACAACC TTTCCCAGTC AAATATGACA
    CAAAAGGTTG AAGAACTAAA GGAAACTGTG AAGGAAGAAT TTATGCCTTG GGTTTCACAG
    TACCTGGTTA TGAAGAGAGT CAGTATTGAG CCAAACTTTC ACAGCCTGTA TTCTAACTTC
    CTTGACACGC TGAAGAATCC TGAGTTTAAT AAGATGGTTC TGAATGAGAC CTATAGAAAC
    ATCAAAGTAC TCCTGACCTC TGATAAAGCT GCAGCCAATT TCTCTGATCG GTCTTTGCTG
    AAGAACTTGG GACATTGGCT AGGAATGATT ACACTAGCTA AAAATAAACC CATCTTACAC
    ACTGACTTGG ATGTAAAATC TTTGCTGCTA GAGGCTTATG TTAAAGGACA GCAAGAGTTG
    CTCTATGTAG TGCCCTTTGT TGCCAAAGTC TTAGAATCTA GCATTCGTAG TGTGGTTTTT
    AGGCCACCCA ACCCCTGGAC AATGGCTATT ATGAATGTAT TAGCTGAGCT ACATCAAGAA
    CATGACTTAA AGTTAAACTT GAAGTTTGAA ATTGAGGTCC TCTGTAAGAA TCTTGCATTA
    GACATCAATG AGCTAAAACC TGGAAACCTC CTAAAGGATA AAGATCGCCT GAAGAACTTA
    GATGAGCAAC TCTCTGCTCC AAAGAAAGAT GTCAAGCAGC CAGAGGAGCT CCCTCCTATC
    ACAACCACAA CAACTTCTAC AACACCAGCT ACCAGCACCA CCTGTACAGC CACTGTTCCA
    CCACAGCCAC AGTACAGTTA CCATGACATC AATGTCTATT CCCTCGCAGG CCTGGCACCA
    CACATTACTC TAAACCCAAC AATTCCCTTG TTTCAGGCCC ATCCACAGCT GAAGCAGTGT
    GTGCGCCAAG CAATCGAACG GGCTGTCCAG GAGCTGGTCC ATCCTGTGGT GGATCGATCA
    ATTAAGATTG CCATGACTAC TTGTGAGCAA ATAGTCAGGA AAGATTTTGC CCTGGATTCT
    GAGGAATCTC GAATGCGAAT AGCAGCTCAT CACATGATGC GGAACTTGAC AGCTGGAATG
    GCTATGATTA CTTGCAGGGA ACCTTTACTC ATGAGCATAT CAACCAACCT GAAAAACAGT
    TTTGCCTCAG CCCTTCGTAC TGCAAGCCCA CAACAAAGGG AAATGATGGA TCAGGCAGCT
    GCTCAATTAG CTCAGGACAA TTGTGAATTA GCTTGCTGTT TTATTCAGAA GACTGCAGTA
    GAAAAAGCAG GCCCTGAGAT GGACAAGAGA TTAGCAACTG AATTTGAGCT GAGAAAACAT
    GCTAGGCAAG AAGGGCGAAG GTATTGTGAT CCTGTTGTGT TAACATACCA AGCTGAGCGG
    ATGCCAGAGC AAATCAGGCT GAAAGTTGGT GGTGTGGACC CAAAGCAGCT GGCTGTTTAT
    GAAGAGTTTG CACGCAATGT GCCTGGCTTC TTGCCTACAA ATGATTTAAG TCAGCCCACA
    GGATTTTTAG CCCAGCCCAT GAAGCAAGCT TGGGCAACAG ATGATGTAGC TCAGATTTAT
    GATAAGTGTA TTACAGAACT GGAGCAGCAT CTTCACGCTA TCCCGCCAAC TTTGGCCATG
    AACCCCCAAG CTCAGGCCCT CAGAAGTCTT CTGGAGGTTG TAGTATTATC ACGGAACTCT
    CGAGATGCCA TTGCTGCTCT TGGATTGCTC CAGAAGGCTG TAGAGGGCTT ACTGGATGCC
    ACCAGTGGTG CTGATGCTGA CCTCCTTCTT CGCTACAGAG AGTGCCACCT CTTGGTCTTG
    AAAGCTCTTC AAGATGGCCG GGCATATGGG TCTCCATGGT GTAACAAACA GATCACAAGG
    TGCCTAATTG AGTGTCGGGA TGAATACAAA TATAATGTGG AGGCAGTGGA GTTGCTAATT
    CGCAATCATT TGGTCAACAT GCAACAGTAT GATCTTCACC TTGCTCAGTC AATGGAGAAT
    GGTTTAAACT ACATGGCTGT GGCATTTGCT ATGCAATTGG TAAAAATACT GCTGGTGGAT
    GAAAGGAGTG TTGCCCATGT TACTGAGGCA GATCTGTTCC ACACCATTGA AACCCTCATG
    AGGATTAATG CTCACTCCAG AGGCAATGCT CCTGAAGGAT TGCCCCAACT GATGGAAGTA
    GTGCGATCCA ACTATGAAGC AATGATTGAT CGTGCTCATG GAGGCCCTAA CTTTATGATG
    CATTCTGGGA TCTCTCAAGC CTCAGAGTAT GATGATCCTC CAGGCCTGAG GGAGAAGGCA
    GAGTATCTTC TGAGGGAATG GGTGAATCTC TACCATTCAG CAGCAGCTGG TCGTGACAGT
    ACCAAAGCTT TCTCTGCGTT TGTTGGACAG GTAGAGCTTT TGGAAAGAAA GATGCACCAG
    CAAGGAATCC TGAAGACTGA TGACCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG
    TGTGTTGAAA TCAGTTACCG TGCTCAGGCA GAGCAGCAGC ACAATCCTGC TGCTAATCCC
    ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTCCGACT CATTGCACTG
    CTAGTAAAGC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAATAAG
    GTCCTCGGTA TAGTAGTGGG AGTTCTCCTA CAGGATCATG ATGTTCGGCA AAGTGAATTT
    CAGCAACTTC CCTACCACCG AATTTTTATC ATGCTGCTCT TGGAACTCAA TGCACCTGAG
    CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAACAC ATTCCACATT
    TTGAGGCCTA CCAAAGCTCC TGGCTTTGTG TATGCTTGGC TTGAACTGAT CTCCCATCGG
    ATATTTATTG CAAGAATGCT GGCACACACG CCACAGCAGA AGGGATGGCC TATGTATGCA
    CAGTTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC
    AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGGGTGC TGCTGGTTCT TTTGCATGAT
    TTTCCTGAGT TCCTTTGTGA TTACCATTAT GGATTTTGTG ACGTGATCCC ACCTAATTGT
    ATCCAGCTAA GAAATCTGAT CCTGAGTGCC TTCCCAAGAA ACATGAGGCT CCCAGACCCA
    TTCACTCCTA ATCTGAAGGT GGATATGTTG AGTGAAATTA ACATCGCTCC CCGGATTCTC
    ACCAATTTTA CTGGAGTGAT GCCACCTCAA TTCAAAAAGG ATTTGGATTC CTATCTTAAA
    ACTCGATCAC CTGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAG
    CCTGGTAATC GCTACAACCT CCAGCTCATT AATGCACTGG TGCTCTATGT AGGGACTCAG
    GCCATTGCAC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACGCACTCA
    GCTCACATGG ACATCTTCCA GAACCTGGCT GTGGATTTGG ACACTGAAGG TCGGTATCTC
    TTCTTGAATG CAATTGCAAA TCAGCTGCGG TACCCAAATA GCCACACTCA CTACTTCAGC
    TGCACCATGC TGTACCTTTT TGCAGAGGCC AACACTGAAG CTATTCAAGA ACAGATCACA
    AGGGTTCTTT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC
    TTCATTGAGC TGATTAAAAA TCCAGCATTT AAATTCTGGA ACCATGAATT TGTACACTGT
    GCCCCAGAAA TTGAAAAGTT ATTCCAGTCC GTCGCACAGT GCTGCATGGG ACAAAAGCAG
    GCTCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225090
    Clone ID Related Accession (Same CDS sequence) XM_024978370.1
    Accession Version XM_024978370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGAGTAAGAT GAAACCTTCG
    GACTTGTCTC AGGTGTGGCC AGAGGCAAAT CAGCACTTTA GTAAAGAGAT AGATGATGAA
    GCAAACAGCT ATTTCCAGAG AATATATAAT CACCCACCAC ATCCAACCAT GTCTGTTGAT
    GAGGTATTAG AAATGCTACA GAGATTTAAA GATTCTACCA TAAAGAGGGA ACGAGAAGTA
    TTTAACTGTA TGCTAAGGAA CTTGTTCGAA GAATATCGGT TCTTCCCCCA ATACCCTGAT
    AAAGAGTTAC ATATAACAGC CTGCCTGTTT GGTGGAATAA TCGAAAAAGG ACTGGTCACT
    TATATGGCAC TAGGTTTGGC CCTGCGATAC GTTCTTGAAG CCTTACGCAA GCCTTTTGGA
    TCCAAAATGT ATTATTTTGG GATTGCTGCA CTAGATAGAT TTAAAAATAG ATTGAAGGAC
    TATCCCCAGT ATTGTCAGCA CTTGGCTTCG ATCAGTCACT TTATGCAATT TCCACATCAT
    TTACAGGAGT ATATTGAGTA TGGACAGCAA TCTAGAGATC CTCCTGTGAA AATGCAGGGC
    TCTATCACAA CCCCTGGAAG TATTGCACTG GCTCAAGCCC AGGCCCAGGC CCAGGTTCCA
    GCAAAAGCTC CCCTTGCTGG CCAGGTTAGC ACTATGGTAA CCACCTCAAC AACCACCACT
    GTTGCTAAAA CGGTTACAGT CACCAGGCCA ACTGGAGTCA GCTTTAAGAA AGATGTGCCA
    CCTTCTATTA ATACAACAAA TATAGATACA TTGCTTGTGG CCACAGATCA AACTGAGAGA
    ATCGTAGAGC CCCCAGAAAA TATCCAGGAG AAAATTGCTT TTATCTTCAA CAACCTTTCC
    CAGTCAAATA TGACACAAAA GGTTGAAGAA CTAAAGGAAA CTGTGAAGGA AGAATTTATG
    CCTTGGGTTT CACAGTACCT GGTTATGAAG AGAGTCAGTA TTGAGCCAAA CTTTCACAGC
    CTGTATTCTA ACTTCCTTGA CACGCTGAAG AATCCTGAGT TTAATAAGAT GGTTCTGAAT
    GAGACCTATA GAAACATCAA AGTACTCCTG ACCTCTGATA AAGCTGCAGC CAATTTCTCT
    GATCGGTCTT TGCTGAAGAA CTTGGGACAT TGGCTAGGAA TGATTACACT AGCTAAAAAT
    AAACCCATCT TACACACTGA CTTGGATGTA AAATCTTTGC TGCTAGAGGC TTATGTTAAA
    GGACAGCAAG AGTTGCTCTA TGTAGTGCCC TTTGTTGCCA AAGTCTTAGA ATCTAGCATT
    CGTAGTGTGG TTTTTAGGCC ACCCAACCCC TGGACAATGG CTATTATGAA TGTATTAGCT
    GAGCTACATC AAGAACATGA CTTAAAGTTA AACTTGAAGT TTGAAATTGA GGTCCTCTGT
    AAGAATCTTG CATTAGACAT CAATGAGCTA AAACCTGGAA ACCTCCTAAA GGATAAAGAT
    CGCCTGAAGA ACTTAGATGA GCAACTCTCT GCTCCAAAGA AAGATGTCAA GCAGCCAGAG
    GAGCTCCCTC CTATCACAAC CACAACAACT TCTACAACAC CAGCTACCAG CACCACCTGT
    ACAGCCACTG TTCCACCACA GCCACAGTAC AGTTACCATG ACATCAATGT CTATTCCCTC
    GCAGGCCTGG CACCACACAT TACTCTAAAC CCAACAATTC CCTTGTTTCA GGCCCATCCA
    CAGCTGAAGC AGTGTGTGCG CCAAGCAATC GAACGGGCTG TCCAGGAGCT GGTCCATCCT
    GTGGTGGATC GATCAATTAA GATTGCCATG ACTACTTGTG AGCAAATAGT CAGGAAAGAT
    TTTGCCCTGG ATTCTGAGGA ATCTCGAATG CGAATAGCAG CTCATCACAT GATGCGGAAC
    TTGACAGCTG GAATGGCTAT GATTACTTGC AGGGAACCTT TACTCATGAG CATATCAACC
    AACCTGAAAA ACAGTTTTGC CTCAGCCCTT CGTACTGCAA GCCCACAACA AAGGGAAATG
    ATGGATCAGG CAGCTGCTCA ATTAGCTCAG GACAATTGTG AATTAGCTTG CTGTTTTATT
    CAGAAGACTG CAGTAGAAAA AGCAGGCCCT GAGATGGACA AGAGATTAGC AACTGAATTT
    GAGCTGAGAA AACATGCTAG GCAAGAAGGG CGAAGGTATT GTGATCCTGT TGTGTTAACA
    TACCAAGCTG AGCGGATGCC AGAGCAAATC AGGCTGAAAG TTGGTGGTGT GGACCCAAAG
    CAGCTGGCTG TTTATGAAGA GTTTGCACGC AATGTGCCTG GCTTCTTGCC TACAAATGAT
    TTAAGTCAGC CCACAGGATT TTTAGCCCAG CCCATGAAGC AAGCTTGGGC AACAGATGAT
    GTAGCTCAGA TTTATGATAA GTGTATTACA GAACTGGAGC AGCATCTTCA CGCTATCCCG
    CCAACTTTGG CCATGAACCC CCAAGCTCAG GCCCTCAGAA GTCTTCTGGA GGTTGTAGTA
    TTATCACGGA ACTCTCGAGA TGCCATTGCT GCTCTTGGAT TGCTCCAGAA GGCTGTAGAG
    GGCTTACTGG ATGCCACCAG TGGTGCTGAT GCTGACCTCC TTCTTCGCTA CAGAGAGTGC
    CACCTCTTGG TCTTGAAAGC TCTTCAAGAT GGCCGGGCAT ATGGGTCTCC ATGGTGTAAC
    AAACAGATCA CAAGGTGCCT AATTGAGTGT CGGGATGAAT ACAAATATAA TGTGGAGGCA
    GTGGAGTTGC TAATTCGCAA TCATTTGGTC AACATGCAAC AGTATGATCT TCACCTTGCT
    CAGTCAATGG AGAATGGTTT AAACTACATG GCTGTGGCAT TTGCTATGCA ATTGGTAAAA
    ATACTGCTGG TGGATGAAAG GAGTGTTGCC CATGTTACTG AGGCAGATCT GTTCCACACC
    ATTGAAACCC TCATGAGGAT TAATGCTCAC TCCAGAGGCA ATGCTCCTGA AGGATTGCCC
    CAACTGATGG AAGTAGTGCG ATCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGC
    CCTAACTTTA TGATGCATTC TGGGATCTCT CAAGCCTCAG AGTATGATGA TCCTCCAGGC
    CTGAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCAGCA
    GCTGGTCGTG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGATGCA CCAGCAAGGA
    ATCCTGAAGA CTGATGACCT CATAACAAGG TTCTTTCGTC TGTGTACTGA AATGTGTGTT
    GAAATCAGTT ACCGTGCTCA GGCAGAGCAG CAGCACAATC CTGCTGCTAA TCCCACCATG
    ATCCGAGCCA AGTGCTATCA CAACCTGGAT GCCTTTGTCC GACTCATTGC ACTGCTAGTA
    AAGCACTCAG GGGAGGCCAC CAACACTGTC ACAAAGATTA ATCTGCTGAA TAAGGTCCTC
    GGTATAGTAG TGGGAGTTCT CCTACAGGAT CATGATGTTC GGCAAAGTGA ATTTCAGCAA
    CTTCCCTACC ACCGAATTTT TATCATGCTG CTCTTGGAAC TCAATGCACC TGAGCATGTG
    TTGGAAACCA TTAATTTCCA GACACTTACA GCTTTCTGCA ACACATTCCA CATTTTGAGG
    CCTACCAAAG CTCCTGGCTT TGTGTATGCT TGGCTTGAAC TGATCTCCCA TCGGATATTT
    ATTGCAAGAA TGCTGGCACA CACGCCACAG CAGAAGGGAT GGCCTATGTA TGCACAGTTA
    CTGATTGATT TATTCAAATA TTTAGCGCCT TTCCTTAGAA ATGTGGAACT CACCAAACCT
    ATGCAAATCC TCTACAAGGG CACTTTAAGG GTGCTGCTGG TTCTTTTGCA TGATTTTCCT
    GAGTTCCTTT GTGATTACCA TTATGGATTT TGTGACGTGA TCCCACCTAA TTGTATCCAG
    CTAAGAAATC TGATCCTGAG TGCCTTCCCA AGAAACATGA GGCTCCCAGA CCCATTCACT
    CCTAATCTGA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCCCGGAT TCTCACCAAT
    TTTACTGGAG TGATGCCACC TCAATTCAAA AAGGATTTGG ATTCCTATCT TAAAACTCGA
    TCACCTGTCA CTTTCCTGTC TGATCTGCGC AGCAACCTAC AGGTATCCAA TGAGCCTGGT
    AATCGCTACA ACCTCCAGCT CATTAATGCA CTGGTGCTCT ATGTAGGGAC TCAGGCCATT
    GCACACATCC ACAACAAGGG CAGCACACCT TCAATGAGCA CCATCACGCA CTCAGCTCAC
    ATGGACATCT TCCAGAACCT GGCTGTGGAT TTGGACACTG AAGGTCGGTA TCTCTTCTTG
    AATGCAATTG CAAATCAGCT GCGGTACCCA AATAGCCACA CTCACTACTT CAGCTGCACC
    ATGCTGTACC TTTTTGCAGA GGCCAACACT GAAGCTATTC AAGAACAGAT CACAAGGGTT
    CTTTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTTCTTAT TACCTTCATT
    GAGCTGATTA AAAATCCAGC ATTTAAATTC TGGAACCATG AATTTGTACA CTGTGCCCCA
    GAAATTGAAA AGTTATTCCA GTCCGTCGCA CAGTGCTGCA TGGGACAAAA GCAGGCTCAG
    CAAGTAATGG AAGGGACAGG TGCCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834138.1
    CDS328..7497
    Translation

    Target ORF information:

    RefSeq Version XM_024978370.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGAGTAAGAT GAAACCTTCG
    GACTTGTCTC AGGTGTGGCC AGAGGCAAAT CAGCACTTTA GTAAAGAGAT AGATGATGAA
    GCAAACAGCT ATTTCCAGAG AATATATAAT CACCCACCAC ATCCAACCAT GTCTGTTGAT
    GAGGTATTAG AAATGCTACA GAGATTTAAA GATTCTACCA TAAAGAGGGA ACGAGAAGTA
    TTTAACTGTA TGCTAAGGAA CTTGTTCGAA GAATATCGGT TCTTCCCCCA ATACCCTGAT
    AAAGAGTTAC ATATAACAGC CTGCCTGTTT GGTGGAATAA TCGAAAAAGG ACTGGTCACT
    TATATGGCAC TAGGTTTGGC CCTGCGATAC GTTCTTGAAG CCTTACGCAA GCCTTTTGGA
    TCCAAAATGT ATTATTTTGG GATTGCTGCA CTAGATAGAT TTAAAAATAG ATTGAAGGAC
    TATCCCCAGT ATTGTCAGCA CTTGGCTTCG ATCAGTCACT TTATGCAATT TCCACATCAT
    TTACAGGAGT ATATTGAGTA TGGACAGCAA TCTAGAGATC CTCCTGTGAA AATGCAGGGC
    TCTATCACAA CCCCTGGAAG TATTGCACTG GCTCAAGCCC AGGCCCAGGC CCAGGTTCCA
    GCAAAAGCTC CCCTTGCTGG CCAGGTTAGC ACTATGGTAA CCACCTCAAC AACCACCACT
    GTTGCTAAAA CGGTTACAGT CACCAGGCCA ACTGGAGTCA GCTTTAAGAA AGATGTGCCA
    CCTTCTATTA ATACAACAAA TATAGATACA TTGCTTGTGG CCACAGATCA AACTGAGAGA
    ATCGTAGAGC CCCCAGAAAA TATCCAGGAG AAAATTGCTT TTATCTTCAA CAACCTTTCC
    CAGTCAAATA TGACACAAAA GGTTGAAGAA CTAAAGGAAA CTGTGAAGGA AGAATTTATG
    CCTTGGGTTT CACAGTACCT GGTTATGAAG AGAGTCAGTA TTGAGCCAAA CTTTCACAGC
    CTGTATTCTA ACTTCCTTGA CACGCTGAAG AATCCTGAGT TTAATAAGAT GGTTCTGAAT
    GAGACCTATA GAAACATCAA AGTACTCCTG ACCTCTGATA AAGCTGCAGC CAATTTCTCT
    GATCGGTCTT TGCTGAAGAA CTTGGGACAT TGGCTAGGAA TGATTACACT AGCTAAAAAT
    AAACCCATCT TACACACTGA CTTGGATGTA AAATCTTTGC TGCTAGAGGC TTATGTTAAA
    GGACAGCAAG AGTTGCTCTA TGTAGTGCCC TTTGTTGCCA AAGTCTTAGA ATCTAGCATT
    CGTAGTGTGG TTTTTAGGCC ACCCAACCCC TGGACAATGG CTATTATGAA TGTATTAGCT
    GAGCTACATC AAGAACATGA CTTAAAGTTA AACTTGAAGT TTGAAATTGA GGTCCTCTGT
    AAGAATCTTG CATTAGACAT CAATGAGCTA AAACCTGGAA ACCTCCTAAA GGATAAAGAT
    CGCCTGAAGA ACTTAGATGA GCAACTCTCT GCTCCAAAGA AAGATGTCAA GCAGCCAGAG
    GAGCTCCCTC CTATCACAAC CACAACAACT TCTACAACAC CAGCTACCAG CACCACCTGT
    ACAGCCACTG TTCCACCACA GCCACAGTAC AGTTACCATG ACATCAATGT CTATTCCCTC
    GCAGGCCTGG CACCACACAT TACTCTAAAC CCAACAATTC CCTTGTTTCA GGCCCATCCA
    CAGCTGAAGC AGTGTGTGCG CCAAGCAATC GAACGGGCTG TCCAGGAGCT GGTCCATCCT
    GTGGTGGATC GATCAATTAA GATTGCCATG ACTACTTGTG AGCAAATAGT CAGGAAAGAT
    TTTGCCCTGG ATTCTGAGGA ATCTCGAATG CGAATAGCAG CTCATCACAT GATGCGGAAC
    TTGACAGCTG GAATGGCTAT GATTACTTGC AGGGAACCTT TACTCATGAG CATATCAACC
    AACCTGAAAA ACAGTTTTGC CTCAGCCCTT CGTACTGCAA GCCCACAACA AAGGGAAATG
    ATGGATCAGG CAGCTGCTCA ATTAGCTCAG GACAATTGTG AATTAGCTTG CTGTTTTATT
    CAGAAGACTG CAGTAGAAAA AGCAGGCCCT GAGATGGACA AGAGATTAGC AACTGAATTT
    GAGCTGAGAA AACATGCTAG GCAAGAAGGG CGAAGGTATT GTGATCCTGT TGTGTTAACA
    TACCAAGCTG AGCGGATGCC AGAGCAAATC AGGCTGAAAG TTGGTGGTGT GGACCCAAAG
    CAGCTGGCTG TTTATGAAGA GTTTGCACGC AATGTGCCTG GCTTCTTGCC TACAAATGAT
    TTAAGTCAGC CCACAGGATT TTTAGCCCAG CCCATGAAGC AAGCTTGGGC AACAGATGAT
    GTAGCTCAGA TTTATGATAA GTGTATTACA GAACTGGAGC AGCATCTTCA CGCTATCCCG
    CCAACTTTGG CCATGAACCC CCAAGCTCAG GCCCTCAGAA GTCTTCTGGA GGTTGTAGTA
    TTATCACGGA ACTCTCGAGA TGCCATTGCT GCTCTTGGAT TGCTCCAGAA GGCTGTAGAG
    GGCTTACTGG ATGCCACCAG TGGTGCTGAT GCTGACCTCC TTCTTCGCTA CAGAGAGTGC
    CACCTCTTGG TCTTGAAAGC TCTTCAAGAT GGCCGGGCAT ATGGGTCTCC ATGGTGTAAC
    AAACAGATCA CAAGGTGCCT AATTGAGTGT CGGGATGAAT ACAAATATAA TGTGGAGGCA
    GTGGAGTTGC TAATTCGCAA TCATTTGGTC AACATGCAAC AGTATGATCT TCACCTTGCT
    CAGTCAATGG AGAATGGTTT AAACTACATG GCTGTGGCAT TTGCTATGCA ATTGGTAAAA
    ATACTGCTGG TGGATGAAAG GAGTGTTGCC CATGTTACTG AGGCAGATCT GTTCCACACC
    ATTGAAACCC TCATGAGGAT TAATGCTCAC TCCAGAGGCA ATGCTCCTGA AGGATTGCCC
    CAACTGATGG AAGTAGTGCG ATCCAACTAT GAAGCAATGA TTGATCGTGC TCATGGAGGC
    CCTAACTTTA TGATGCATTC TGGGATCTCT CAAGCCTCAG AGTATGATGA TCCTCCAGGC
    CTGAGGGAGA AGGCAGAGTA TCTTCTGAGG GAATGGGTGA ATCTCTACCA TTCAGCAGCA
    GCTGGTCGTG ACAGTACCAA AGCTTTCTCT GCGTTTGTTG GACAGATGCA CCAGCAAGGA
    ATCCTGAAGA CTGATGACCT CATAACAAGG TTCTTTCGTC TGTGTACTGA AATGTGTGTT
    GAAATCAGTT ACCGTGCTCA GGCAGAGCAG CAGCACAATC CTGCTGCTAA TCCCACCATG
    ATCCGAGCCA AGTGCTATCA CAACCTGGAT GCCTTTGTCC GACTCATTGC ACTGCTAGTA
    AAGCACTCAG GGGAGGCCAC CAACACTGTC ACAAAGATTA ATCTGCTGAA TAAGGTCCTC
    GGTATAGTAG TGGGAGTTCT CCTACAGGAT CATGATGTTC GGCAAAGTGA ATTTCAGCAA
    CTTCCCTACC ACCGAATTTT TATCATGCTG CTCTTGGAAC TCAATGCACC TGAGCATGTG
    TTGGAAACCA TTAATTTCCA GACACTTACA GCTTTCTGCA ACACATTCCA CATTTTGAGG
    CCTACCAAAG CTCCTGGCTT TGTGTATGCT TGGCTTGAAC TGATCTCCCA TCGGATATTT
    ATTGCAAGAA TGCTGGCACA CACGCCACAG CAGAAGGGAT GGCCTATGTA TGCACAGTTA
    CTGATTGATT TATTCAAATA TTTAGCGCCT TTCCTTAGAA ATGTGGAACT CACCAAACCT
    ATGCAAATCC TCTACAAGGG CACTTTAAGG GTGCTGCTGG TTCTTTTGCA TGATTTTCCT
    GAGTTCCTTT GTGATTACCA TTATGGATTT TGTGACGTGA TCCCACCTAA TTGTATCCAG
    CTAAGAAATC TGATCCTGAG TGCCTTCCCA AGAAACATGA GGCTCCCAGA CCCATTCACT
    CCTAATCTGA AGGTGGATAT GTTGAGTGAA ATTAACATCG CTCCCCGGAT TCTCACCAAT
    TTTACTGGAG TGATGCCACC TCAATTCAAA AAGGATTTGG ATTCCTATCT TAAAACTCGA
    TCACCTGTCA CTTTCCTGTC TGATCTGCGC AGCAACCTAC AGGTATCCAA TGAGCCTGGT
    AATCGCTACA ACCTCCAGCT CATTAATGCA CTGGTGCTCT ATGTAGGGAC TCAGGCCATT
    GCACACATCC ACAACAAGGG CAGCACACCT TCAATGAGCA CCATCACGCA CTCAGCTCAC
    ATGGACATCT TCCAGAACCT GGCTGTGGAT TTGGACACTG AAGGTCGGTA TCTCTTCTTG
    AATGCAATTG CAAATCAGCT GCGGTACCCA AATAGCCACA CTCACTACTT CAGCTGCACC
    ATGCTGTACC TTTTTGCAGA GGCCAACACT GAAGCTATTC AAGAACAGAT CACAAGGGTT
    CTTTTGGAAC GGTTGATTGT AAATAGGCCA CATCCTTGGG GTCTTCTTAT TACCTTCATT
    GAGCTGATTA AAAATCCAGC ATTTAAATTC TGGAACCATG AATTTGTACA CTGTGCCCCA
    GAAATTGAAA AGTTATTCCA GTCCGTCGCA CAGTGCTGCA TGGGACAAAA GCAGGCTCAG
    CAAGTAATGG AAGGGACAGG TGCCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225091
    Clone ID Related Accession (Same CDS sequence) XM_024978371.1
    Accession Version XM_024978371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GGTGGTCTTG GCACAGGCAG CCTGACTGGT
    ATAGGAACTG GGGCTCTTGG ACTCCCTGCA GTGAATAGCG ATCCTTTTGT ACAGAGGAAA
    CTGGGCACCT CTGGACTGAA CCAGCCTACA TTCCAGCAGA GTAAGATGAA ACCTTCGGAC
    TTGTCTCAGG TGTGGCCAGA GGCAAATCAG CACTTTAGTA AAGAGATAGA TGATGAAGCA
    AACAGCTATT TCCAGAGAAT ATATAATCAC CCACCACATC CAACCATGTC TGTTGATGAG
    GTATTAGAAA TGCTACAGAG ATTTAAAGAT TCTACCATAA AGAGGGAACG AGAAGTATTT
    AACTGTATGC TAAGGAACTT GTTCGAAGAA TATCGGTTCT TCCCCCAATA CCCTGATAAA
    GAGTTACATA TAACAGCCTG CCTGTTTGGT GGAATAATCG AAAAAGGACT GGTCACTTAT
    ATGGCACTAG GTTTGGCCCT GCGATACGTT CTTGAAGCCT TACGCAAGCC TTTTGGATCC
    AAAATGTATT ATTTTGGGAT TGCTGCACTA GATAGATTTA AAAATAGATT GAAGGACTAT
    CCCCAGTATT GTCAGCACTT GGCTTCGATC AGTCACTTTA TGCAATTTCC ACATCATTTA
    CAGGAGTATA TTGAGTATGG ACAGCAATCT AGAGATCCTC CTGTGAAAAT GCAGGGCTCT
    ATCACAACCC CTGGAAGTAT TGCACTGGCT CAAGCCCAGG CCCAGGCCCA GGTTCCAGCA
    AAAGCTCCCC TTGCTGGCCA GGTTAGCACT ATGGTAACCA CCTCAACAAC CACCACTGTT
    GCTAAAACGG TTACAGTCAC CAGGCCAACT GGAGTCAGCT TTAAGAAAGA TGTGCCACCT
    TCTATTAATA CAACAAATAT AGATACATTG CTTGTGGCCA CAGATCAAAC TGAGAGAATC
    GTAGAGCCCC CAGAAAATAT CCAGGAGAAA ATTGCTTTTA TCTTCAACAA CCTTTCCCAG
    TCAAATATGA CACAAAAGGT TGAAGAACTA AAGGAAACTG TGAAGGAAGA ATTTATGCCT
    TGGGTTTCAC AGTACCTGGT TATGAAGAGA GTCAGTATTG AGCCAAACTT TCACAGCCTG
    TATTCTAACT TCCTTGACAC GCTGAAGAAT CCTGAGTTTA ATAAGATGGT TCTGAATGAG
    ACCTATAGAA ACATCAAAGT ACTCCTGACC TCTGATAAAG CTGCAGCCAA TTTCTCTGAT
    CGGTCTTTGC TGAAGAACTT GGGACATTGG CTAGGAATGA TTACACTAGC TAAAAATAAA
    CCCATCTTAC ACACTGACTT GGATGTAAAA TCTTTGCTGC TAGAGGCTTA TGTTAAAGGA
    CAGCAAGAGT TGCTCTATGT AGTGCCCTTT GTTGCCAAAG TCTTAGAATC TAGCATTCGT
    AGTGTGGTTT TTAGGCCACC CAACCCCTGG ACAATGGCTA TTATGAATGT ATTAGCTGAG
    CTACATCAAG AACATGACTT AAAGTTAAAC TTGAAGTTTG AAATTGAGGT CCTCTGTAAG
    AATCTTGCAT TAGACATCAA TGAGCTAAAA CCTGGAAACC TCCTAAAGGA TAAAGATCGC
    CTGAAGAACT TAGATGAGCA ACTCTCTGCT CCAAAGAAAG ATGTCAAGCA GCCAGAGGAG
    CTCCCTCCTA TCACAACCAC AACAACTTCT ACAACACCAG CTACCAGCAC CACCTGTACA
    GCCACTGTTC CACCACAGCC ACAGTACAGT TACCATGACA TCAATGTCTA TTCCCTCGCA
    GGCCTGGCAC CACACATTAC TCTAAACCCA ACAATTCCCT TGTTTCAGGC CCATCCACAG
    CTGAAGCAGT GTGTGCGCCA AGCAATCGAA CGGGCTGTCC AGGAGCTGGT CCATCCTGTG
    GTGGATCGAT CAATTAAGAT TGCCATGACT ACTTGTGAGC AAATAGTCAG GAAAGATTTT
    GCCCTGGATT CTGAGGAATC TCGAATGCGA ATAGCAGCTC ATCACATGAT GCGGAACTTG
    ACAGCTGGAA TGGCTATGAT TACTTGCAGG GAACCTTTAC TCATGAGCAT ATCAACCAAC
    CTGAAAAACA GTTTTGCCTC AGCCCTTCGT ACTGCAAGCC CACAACAAAG GGAAATGATG
    GATCAGGCAG CTGCTCAATT AGCTCAGGAC AATTGTGAAT TAGCTTGCTG TTTTATTCAG
    AAGACTGCAG TAGAAAAAGC AGGCCCTGAG ATGGACAAGA GATTAGCAAC TGAATTTGAG
    CTGAGAAAAC ATGCTAGGCA AGAAGGGCGA AGGTATTGTG ATCCTGTTGT GTTAACATAC
    CAAGCTGAGC GGATGCCAGA GCAAATCAGG CTGAAAGTTG GTGGTGTGGA CCCAAAGCAG
    CTGGCTGTTT ATGAAGAGTT TGCACGCAAT GTGCCTGGCT TCTTGCCTAC AAATGATTTA
    AGTCAGCCCA CAGGATTTTT AGCCCAGCCC ATGAAGCAAG CTTGGGCAAC AGATGATGTA
    GCTCAGATTT ATGATAAGTG TATTACAGAA CTGGAGCAGC ATCTTCACGC TATCCCGCCA
    ACTTTGGCCA TGAACCCCCA AGCTCAGGCC CTCAGAAGTC TTCTGGAGGT TGTAGTATTA
    TCACGGAACT CTCGAGATGC CATTGCTGCT CTTGGATTGC TCCAGAAGGC TGTAGAGGGC
    TTACTGGATG CCACCAGTGG TGCTGATGCT GACCTCCTTC TTCGCTACAG AGAGTGCCAC
    CTCTTGGTCT TGAAAGCTCT TCAAGATGGC CGGGCATATG GGTCTCCATG GTGTAACAAA
    CAGATCACAA GGTGCCTAAT TGAGTGTCGG GATGAATACA AATATAATGT GGAGGCAGTG
    GAGTTGCTAA TTCGCAATCA TTTGGTCAAC ATGCAACAGT ATGATCTTCA CCTTGCTCAG
    TCAATGGAGA ATGGTTTAAA CTACATGGCT GTGGCATTTG CTATGCAATT GGTAAAAATA
    CTGCTGGTGG ATGAAAGGAG TGTTGCCCAT GTTACTGAGG CAGATCTGTT CCACACCATT
    GAAACCCTCA TGAGGATTAA TGCTCACTCC AGAGGCAATG CTCCTGAAGG ATTGCCCCAA
    CTGATGGAAG TAGTGCGATC CAACTATGAA GCAATGATTG ATCGTGCTCA TGGAGGCCCT
    AACTTTATGA TGCATTCTGG GATCTCTCAA GCCTCAGAGT ATGATGATCC TCCAGGCCTG
    AGGGAGAAGG CAGAGTATCT TCTGAGGGAA TGGGTGAATC TCTACCATTC AGCAGCAGCT
    GGTCGTGACA GTACCAAAGC TTTCTCTGCG TTTGTTGGAC AGATGCACCA GCAAGGAATC
    CTGAAGACTG ATGACCTCAT AACAAGGTTC TTTCGTCTGT GTACTGAAAT GTGTGTTGAA
    ATCAGTTACC GTGCTCAGGC AGAGCAGCAG CACAATCCTG CTGCTAATCC CACCATGATC
    CGAGCCAAGT GCTATCACAA CCTGGATGCC TTTGTCCGAC TCATTGCACT GCTAGTAAAG
    CACTCAGGGG AGGCCACCAA CACTGTCACA AAGATTAATC TGCTGAATAA GGTCCTCGGT
    ATAGTAGTGG GAGTTCTCCT ACAGGATCAT GATGTTCGGC AAAGTGAATT TCAGCAACTT
    CCCTACCACC GAATTTTTAT CATGCTGCTC TTGGAACTCA ATGCACCTGA GCATGTGTTG
    GAAACCATTA ATTTCCAGAC ACTTACAGCT TTCTGCAACA CATTCCACAT TTTGAGGCCT
    ACCAAAGCTC CTGGCTTTGT GTATGCTTGG CTTGAACTGA TCTCCCATCG GATATTTATT
    GCAAGAATGC TGGCACACAC GCCACAGCAG AAGGGATGGC CTATGTATGC ACAGTTACTG
    ATTGATTTAT TCAAATATTT AGCGCCTTTC CTTAGAAATG TGGAACTCAC CAAACCTATG
    CAAATCCTCT ACAAGGGCAC TTTAAGGGTG CTGCTGGTTC TTTTGCATGA TTTTCCTGAG
    TTCCTTTGTG ATTACCATTA TGGATTTTGT GACGTGATCC CACCTAATTG TATCCAGCTA
    AGAAATCTGA TCCTGAGTGC CTTCCCAAGA AACATGAGGC TCCCAGACCC ATTCACTCCT
    AATCTGAAGG TGGATATGTT GAGTGAAATT AACATCGCTC CCCGGATTCT CACCAATTTT
    ACTGGAGTGA TGCCACCTCA ATTCAAAAAG GATTTGGATT CCTATCTTAA AACTCGATCA
    CCTGTCACTT TCCTGTCTGA TCTGCGCAGC AACCTACAGG TATCCAATGA GCCTGGTAAT
    CGCTACAACC TCCAGCTCAT TAATGCACTG GTGCTCTATG TAGGGACTCA GGCCATTGCA
    CACATCCACA ACAAGGGCAG CACACCTTCA ATGAGCACCA TCACGCACTC AGCTCACATG
    GACATCTTCC AGAACCTGGC TGTGGATTTG GACACTGAAG GTCGGTATCT CTTCTTGAAT
    GCAATTGCAA ATCAGCTGCG GTACCCAAAT AGCCACACTC ACTACTTCAG CTGCACCATG
    CTGTACCTTT TTGCAGAGGC CAACACTGAA GCTATTCAAG AACAGATCAC AAGGGTTCTT
    TTGGAACGGT TGATTGTAAA TAGGCCACAT CCTTGGGGTC TTCTTATTAC CTTCATTGAG
    CTGATTAAAA ATCCAGCATT TAAATTCTGG AACCATGAAT TTGTACACTG TGCCCCAGAA
    ATTGAAAAGT TATTCCAGTC CGTCGCACAG TGCTGCATGG GACAAAAGCA GGCTCAGCAA
    GTAATGGAAG GGACAGGTGC CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834139.1
    CDS328..7494
    Translation

    Target ORF information:

    RefSeq Version XM_024978371.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GGTGGTCTTG GCACAGGCAG CCTGACTGGT
    ATAGGAACTG GGGCTCTTGG ACTCCCTGCA GTGAATAGCG ATCCTTTTGT ACAGAGGAAA
    CTGGGCACCT CTGGACTGAA CCAGCCTACA TTCCAGCAGA GTAAGATGAA ACCTTCGGAC
    TTGTCTCAGG TGTGGCCAGA GGCAAATCAG CACTTTAGTA AAGAGATAGA TGATGAAGCA
    AACAGCTATT TCCAGAGAAT ATATAATCAC CCACCACATC CAACCATGTC TGTTGATGAG
    GTATTAGAAA TGCTACAGAG ATTTAAAGAT TCTACCATAA AGAGGGAACG AGAAGTATTT
    AACTGTATGC TAAGGAACTT GTTCGAAGAA TATCGGTTCT TCCCCCAATA CCCTGATAAA
    GAGTTACATA TAACAGCCTG CCTGTTTGGT GGAATAATCG AAAAAGGACT GGTCACTTAT
    ATGGCACTAG GTTTGGCCCT GCGATACGTT CTTGAAGCCT TACGCAAGCC TTTTGGATCC
    AAAATGTATT ATTTTGGGAT TGCTGCACTA GATAGATTTA AAAATAGATT GAAGGACTAT
    CCCCAGTATT GTCAGCACTT GGCTTCGATC AGTCACTTTA TGCAATTTCC ACATCATTTA
    CAGGAGTATA TTGAGTATGG ACAGCAATCT AGAGATCCTC CTGTGAAAAT GCAGGGCTCT
    ATCACAACCC CTGGAAGTAT TGCACTGGCT CAAGCCCAGG CCCAGGCCCA GGTTCCAGCA
    AAAGCTCCCC TTGCTGGCCA GGTTAGCACT ATGGTAACCA CCTCAACAAC CACCACTGTT
    GCTAAAACGG TTACAGTCAC CAGGCCAACT GGAGTCAGCT TTAAGAAAGA TGTGCCACCT
    TCTATTAATA CAACAAATAT AGATACATTG CTTGTGGCCA CAGATCAAAC TGAGAGAATC
    GTAGAGCCCC CAGAAAATAT CCAGGAGAAA ATTGCTTTTA TCTTCAACAA CCTTTCCCAG
    TCAAATATGA CACAAAAGGT TGAAGAACTA AAGGAAACTG TGAAGGAAGA ATTTATGCCT
    TGGGTTTCAC AGTACCTGGT TATGAAGAGA GTCAGTATTG AGCCAAACTT TCACAGCCTG
    TATTCTAACT TCCTTGACAC GCTGAAGAAT CCTGAGTTTA ATAAGATGGT TCTGAATGAG
    ACCTATAGAA ACATCAAAGT ACTCCTGACC TCTGATAAAG CTGCAGCCAA TTTCTCTGAT
    CGGTCTTTGC TGAAGAACTT GGGACATTGG CTAGGAATGA TTACACTAGC TAAAAATAAA
    CCCATCTTAC ACACTGACTT GGATGTAAAA TCTTTGCTGC TAGAGGCTTA TGTTAAAGGA
    CAGCAAGAGT TGCTCTATGT AGTGCCCTTT GTTGCCAAAG TCTTAGAATC TAGCATTCGT
    AGTGTGGTTT TTAGGCCACC CAACCCCTGG ACAATGGCTA TTATGAATGT ATTAGCTGAG
    CTACATCAAG AACATGACTT AAAGTTAAAC TTGAAGTTTG AAATTGAGGT CCTCTGTAAG
    AATCTTGCAT TAGACATCAA TGAGCTAAAA CCTGGAAACC TCCTAAAGGA TAAAGATCGC
    CTGAAGAACT TAGATGAGCA ACTCTCTGCT CCAAAGAAAG ATGTCAAGCA GCCAGAGGAG
    CTCCCTCCTA TCACAACCAC AACAACTTCT ACAACACCAG CTACCAGCAC CACCTGTACA
    GCCACTGTTC CACCACAGCC ACAGTACAGT TACCATGACA TCAATGTCTA TTCCCTCGCA
    GGCCTGGCAC CACACATTAC TCTAAACCCA ACAATTCCCT TGTTTCAGGC CCATCCACAG
    CTGAAGCAGT GTGTGCGCCA AGCAATCGAA CGGGCTGTCC AGGAGCTGGT CCATCCTGTG
    GTGGATCGAT CAATTAAGAT TGCCATGACT ACTTGTGAGC AAATAGTCAG GAAAGATTTT
    GCCCTGGATT CTGAGGAATC TCGAATGCGA ATAGCAGCTC ATCACATGAT GCGGAACTTG
    ACAGCTGGAA TGGCTATGAT TACTTGCAGG GAACCTTTAC TCATGAGCAT ATCAACCAAC
    CTGAAAAACA GTTTTGCCTC AGCCCTTCGT ACTGCAAGCC CACAACAAAG GGAAATGATG
    GATCAGGCAG CTGCTCAATT AGCTCAGGAC AATTGTGAAT TAGCTTGCTG TTTTATTCAG
    AAGACTGCAG TAGAAAAAGC AGGCCCTGAG ATGGACAAGA GATTAGCAAC TGAATTTGAG
    CTGAGAAAAC ATGCTAGGCA AGAAGGGCGA AGGTATTGTG ATCCTGTTGT GTTAACATAC
    CAAGCTGAGC GGATGCCAGA GCAAATCAGG CTGAAAGTTG GTGGTGTGGA CCCAAAGCAG
    CTGGCTGTTT ATGAAGAGTT TGCACGCAAT GTGCCTGGCT TCTTGCCTAC AAATGATTTA
    AGTCAGCCCA CAGGATTTTT AGCCCAGCCC ATGAAGCAAG CTTGGGCAAC AGATGATGTA
    GCTCAGATTT ATGATAAGTG TATTACAGAA CTGGAGCAGC ATCTTCACGC TATCCCGCCA
    ACTTTGGCCA TGAACCCCCA AGCTCAGGCC CTCAGAAGTC TTCTGGAGGT TGTAGTATTA
    TCACGGAACT CTCGAGATGC CATTGCTGCT CTTGGATTGC TCCAGAAGGC TGTAGAGGGC
    TTACTGGATG CCACCAGTGG TGCTGATGCT GACCTCCTTC TTCGCTACAG AGAGTGCCAC
    CTCTTGGTCT TGAAAGCTCT TCAAGATGGC CGGGCATATG GGTCTCCATG GTGTAACAAA
    CAGATCACAA GGTGCCTAAT TGAGTGTCGG GATGAATACA AATATAATGT GGAGGCAGTG
    GAGTTGCTAA TTCGCAATCA TTTGGTCAAC ATGCAACAGT ATGATCTTCA CCTTGCTCAG
    TCAATGGAGA ATGGTTTAAA CTACATGGCT GTGGCATTTG CTATGCAATT GGTAAAAATA
    CTGCTGGTGG ATGAAAGGAG TGTTGCCCAT GTTACTGAGG CAGATCTGTT CCACACCATT
    GAAACCCTCA TGAGGATTAA TGCTCACTCC AGAGGCAATG CTCCTGAAGG ATTGCCCCAA
    CTGATGGAAG TAGTGCGATC CAACTATGAA GCAATGATTG ATCGTGCTCA TGGAGGCCCT
    AACTTTATGA TGCATTCTGG GATCTCTCAA GCCTCAGAGT ATGATGATCC TCCAGGCCTG
    AGGGAGAAGG CAGAGTATCT TCTGAGGGAA TGGGTGAATC TCTACCATTC AGCAGCAGCT
    GGTCGTGACA GTACCAAAGC TTTCTCTGCG TTTGTTGGAC AGATGCACCA GCAAGGAATC
    CTGAAGACTG ATGACCTCAT AACAAGGTTC TTTCGTCTGT GTACTGAAAT GTGTGTTGAA
    ATCAGTTACC GTGCTCAGGC AGAGCAGCAG CACAATCCTG CTGCTAATCC CACCATGATC
    CGAGCCAAGT GCTATCACAA CCTGGATGCC TTTGTCCGAC TCATTGCACT GCTAGTAAAG
    CACTCAGGGG AGGCCACCAA CACTGTCACA AAGATTAATC TGCTGAATAA GGTCCTCGGT
    ATAGTAGTGG GAGTTCTCCT ACAGGATCAT GATGTTCGGC AAAGTGAATT TCAGCAACTT
    CCCTACCACC GAATTTTTAT CATGCTGCTC TTGGAACTCA ATGCACCTGA GCATGTGTTG
    GAAACCATTA ATTTCCAGAC ACTTACAGCT TTCTGCAACA CATTCCACAT TTTGAGGCCT
    ACCAAAGCTC CTGGCTTTGT GTATGCTTGG CTTGAACTGA TCTCCCATCG GATATTTATT
    GCAAGAATGC TGGCACACAC GCCACAGCAG AAGGGATGGC CTATGTATGC ACAGTTACTG
    ATTGATTTAT TCAAATATTT AGCGCCTTTC CTTAGAAATG TGGAACTCAC CAAACCTATG
    CAAATCCTCT ACAAGGGCAC TTTAAGGGTG CTGCTGGTTC TTTTGCATGA TTTTCCTGAG
    TTCCTTTGTG ATTACCATTA TGGATTTTGT GACGTGATCC CACCTAATTG TATCCAGCTA
    AGAAATCTGA TCCTGAGTGC CTTCCCAAGA AACATGAGGC TCCCAGACCC ATTCACTCCT
    AATCTGAAGG TGGATATGTT GAGTGAAATT AACATCGCTC CCCGGATTCT CACCAATTTT
    ACTGGAGTGA TGCCACCTCA ATTCAAAAAG GATTTGGATT CCTATCTTAA AACTCGATCA
    CCTGTCACTT TCCTGTCTGA TCTGCGCAGC AACCTACAGG TATCCAATGA GCCTGGTAAT
    CGCTACAACC TCCAGCTCAT TAATGCACTG GTGCTCTATG TAGGGACTCA GGCCATTGCA
    CACATCCACA ACAAGGGCAG CACACCTTCA ATGAGCACCA TCACGCACTC AGCTCACATG
    GACATCTTCC AGAACCTGGC TGTGGATTTG GACACTGAAG GTCGGTATCT CTTCTTGAAT
    GCAATTGCAA ATCAGCTGCG GTACCCAAAT AGCCACACTC ACTACTTCAG CTGCACCATG
    CTGTACCTTT TTGCAGAGGC CAACACTGAA GCTATTCAAG AACAGATCAC AAGGGTTCTT
    TTGGAACGGT TGATTGTAAA TAGGCCACAT CCTTGGGGTC TTCTTATTAC CTTCATTGAG
    CTGATTAAAA ATCCAGCATT TAAATTCTGG AACCATGAAT TTGTACACTG TGCCCCAGAA
    ATTGAAAAGT TATTCCAGTC CGTCGCACAG TGCTGCATGG GACAAAAGCA GGCTCAGCAA
    GTAATGGAAG GGACAGGTGC CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225092
    Clone ID Related Accession (Same CDS sequence) XM_024978372.1
    Accession Version XM_024978372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGACGGACTT GTCTCAGGTG
    TGGCCAGAGG CAAATCAGCA CTTTAGTAAA GAGATAGATG ATGAAGCAAA CAGCTATTTC
    CAGAGAATAT ATAATCACCC ACCACATCCA ACCATGTCTG TTGATGAGGT ATTAGAAATG
    CTACAGAGAT TTAAAGATTC TACCATAAAG AGGGAACGAG AAGTATTTAA CTGTATGCTA
    AGGAACTTGT TCGAAGAATA TCGGTTCTTC CCCCAATACC CTGATAAAGA GTTACATATA
    ACAGCCTGCC TGTTTGGTGG AATAATCGAA AAAGGACTGG TCACTTATAT GGCACTAGGT
    TTGGCCCTGC GATACGTTCT TGAAGCCTTA CGCAAGCCTT TTGGATCCAA AATGTATTAT
    TTTGGGATTG CTGCACTAGA TAGATTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT
    CAGCACTTGG CTTCGATCAG TCACTTTATG CAATTTCCAC ATCATTTACA GGAGTATATT
    GAGTATGGAC AGCAATCTAG AGATCCTCCT GTGAAAATGC AGGGCTCTAT CACAACCCCT
    GGAAGTATTG CACTGGCTCA AGCCCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCCCTT
    GCTGGCCAGG TTAGCACTAT GGTAACCACC TCAACAACCA CCACTGTTGC TAAAACGGTT
    ACAGTCACCA GGCCAACTGG AGTCAGCTTT AAGAAAGATG TGCCACCTTC TATTAATACA
    ACAAATATAG ATACATTGCT TGTGGCCACA GATCAAACTG AGAGAATCGT AGAGCCCCCA
    GAAAATATCC AGGAGAAAAT TGCTTTTATC TTCAACAACC TTTCCCAGTC AAATATGACA
    CAAAAGGTTG AAGAACTAAA GGAAACTGTG AAGGAAGAAT TTATGCCTTG GGTTTCACAG
    TACCTGGTTA TGAAGAGAGT CAGTATTGAG CCAAACTTTC ACAGCCTGTA TTCTAACTTC
    CTTGACACGC TGAAGAATCC TGAGTTTAAT AAGATGGTTC TGAATGAGAC CTATAGAAAC
    ATCAAAGTAC TCCTGACCTC TGATAAAGCT GCAGCCAATT TCTCTGATCG GTCTTTGCTG
    AAGAACTTGG GACATTGGCT AGGAATGATT ACACTAGCTA AAAATAAACC CATCTTACAC
    ACTGACTTGG ATGTAAAATC TTTGCTGCTA GAGGCTTATG TTAAAGGACA GCAAGAGTTG
    CTCTATGTAG TGCCCTTTGT TGCCAAAGTC TTAGAATCTA GCATTCGTAG TGTGGTTTTT
    AGGCCACCCA ACCCCTGGAC AATGGCTATT ATGAATGTAT TAGCTGAGCT ACATCAAGAA
    CATGACTTAA AGTTAAACTT GAAGTTTGAA ATTGAGGTCC TCTGTAAGAA TCTTGCATTA
    GACATCAATG AGCTAAAACC TGGAAACCTC CTAAAGGATA AAGATCGCCT GAAGAACTTA
    GATGAGCAAC TCTCTGCTCC AAAGAAAGAT GTCAAGCAGC CAGAGGAGCT CCCTCCTATC
    ACAACCACAA CAACTTCTAC AACACCAGCT ACCAGCACCA CCTGTACAGC CACTGTTCCA
    CCACAGCCAC AGTACAGTTA CCATGACATC AATGTCTATT CCCTCGCAGG CCTGGCACCA
    CACATTACTC TAAACCCAAC AATTCCCTTG TTTCAGGCCC ATCCACAGCT GAAGCAGTGT
    GTGCGCCAAG CAATCGAACG GGCTGTCCAG GAGCTGGTCC ATCCTGTGGT GGATCGATCA
    ATTAAGATTG CCATGACTAC TTGTGAGCAA ATAGTCAGGA AAGATTTTGC CCTGGATTCT
    GAGGAATCTC GAATGCGAAT AGCAGCTCAT CACATGATGC GGAACTTGAC AGCTGGAATG
    GCTATGATTA CTTGCAGGGA ACCTTTACTC ATGAGCATAT CAACCAACCT GAAAAACAGT
    TTTGCCTCAG CCCTTCGTAC TGCAAGCCCA CAACAAAGGG AAATGATGGA TCAGGCAGCT
    GCTCAATTAG CTCAGGACAA TTGTGAATTA GCTTGCTGTT TTATTCAGAA GACTGCAGTA
    GAAAAAGCAG GCCCTGAGAT GGACAAGAGA TTAGCAACTG AATTTGAGCT GAGAAAACAT
    GCTAGGCAAG AAGGGCGAAG GTATTGTGAT CCTGTTGTGT TAACATACCA AGCTGAGCGG
    ATGCCAGAGC AAATCAGGCT GAAAGTTGGT GGTGTGGACC CAAAGCAGCT GGCTGTTTAT
    GAAGAGTTTG CACGCAATGT GCCTGGCTTC TTGCCTACAA ATGATTTAAG TCAGCCCACA
    GGATTTTTAG CCCAGCCCAT GAAGCAAGCT TGGGCAACAG ATGATGTAGC TCAGATTTAT
    GATAAGTGTA TTACAGAACT GGAGCAGCAT CTTCACGCTA TCCCGCCAAC TTTGGCCATG
    AACCCCCAAG CTCAGGCCCT CAGAAGTCTT CTGGAGGTTG TAGTATTATC ACGGAACTCT
    CGAGATGCCA TTGCTGCTCT TGGATTGCTC CAGAAGGCTG TAGAGGGCTT ACTGGATGCC
    ACCAGTGGTG CTGATGCTGA CCTCCTTCTT CGCTACAGAG AGTGCCACCT CTTGGTCTTG
    AAAGCTCTTC AAGATGGCCG GGCATATGGG TCTCCATGGT GTAACAAACA GATCACAAGG
    TGCCTAATTG AGTGTCGGGA TGAATACAAA TATAATGTGG AGGCAGTGGA GTTGCTAATT
    CGCAATCATT TGGTCAACAT GCAACAGTAT GATCTTCACC TTGCTCAGTC AATGGAGAAT
    GGTTTAAACT ACATGGCTGT GGCATTTGCT ATGCAATTGG TAAAAATACT GCTGGTGGAT
    GAAAGGAGTG TTGCCCATGT TACTGAGGCA GATCTGTTCC ACACCATTGA AACCCTCATG
    AGGATTAATG CTCACTCCAG AGGCAATGCT CCTGAAGGAT TGCCCCAACT GATGGAAGTA
    GTGCGATCCA ACTATGAAGC AATGATTGAT CGTGCTCATG GAGGCCCTAA CTTTATGATG
    CATTCTGGGA TCTCTCAAGC CTCAGAGTAT GATGATCCTC CAGGCCTGAG GGAGAAGGCA
    GAGTATCTTC TGAGGGAATG GGTGAATCTC TACCATTCAG CAGCAGCTGG TCGTGACAGT
    ACCAAAGCTT TCTCTGCGTT TGTTGGACAG ATGCACCAGC AAGGAATCCT GAAGACTGAT
    GACCTCATAA CAAGGTTCTT TCGTCTGTGT ACTGAAATGT GTGTTGAAAT CAGTTACCGT
    GCTCAGGCAG AGCAGCAGCA CAATCCTGCT GCTAATCCCA CCATGATCCG AGCCAAGTGC
    TATCACAACC TGGATGCCTT TGTCCGACTC ATTGCACTGC TAGTAAAGCA CTCAGGGGAG
    GCCACCAACA CTGTCACAAA GATTAATCTG CTGAATAAGG TCCTCGGTAT AGTAGTGGGA
    GTTCTCCTAC AGGATCATGA TGTTCGGCAA AGTGAATTTC AGCAACTTCC CTACCACCGA
    ATTTTTATCA TGCTGCTCTT GGAACTCAAT GCACCTGAGC ATGTGTTGGA AACCATTAAT
    TTCCAGACAC TTACAGCTTT CTGCAACACA TTCCACATTT TGAGGCCTAC CAAAGCTCCT
    GGCTTTGTGT ATGCTTGGCT TGAACTGATC TCCCATCGGA TATTTATTGC AAGAATGCTG
    GCACACACGC CACAGCAGAA GGGATGGCCT ATGTATGCAC AGTTACTGAT TGATTTATTC
    AAATATTTAG CGCCTTTCCT TAGAAATGTG GAACTCACCA AACCTATGCA AATCCTCTAC
    AAGGGCACTT TAAGGGTGCT GCTGGTTCTT TTGCATGATT TTCCTGAGTT CCTTTGTGAT
    TACCATTATG GATTTTGTGA CGTGATCCCA CCTAATTGTA TCCAGCTAAG AAATCTGATC
    CTGAGTGCCT TCCCAAGAAA CATGAGGCTC CCAGACCCAT TCACTCCTAA TCTGAAGGTG
    GATATGTTGA GTGAAATTAA CATCGCTCCC CGGATTCTCA CCAATTTTAC TGGAGTGATG
    CCACCTCAAT TCAAAAAGGA TTTGGATTCC TATCTTAAAA CTCGATCACC TGTCACTTTC
    CTGTCTGATC TGCGCAGCAA CCTACAGGTA TCCAATGAGC CTGGTAATCG CTACAACCTC
    CAGCTCATTA ATGCACTGGT GCTCTATGTA GGGACTCAGG CCATTGCACA CATCCACAAC
    AAGGGCAGCA CACCTTCAAT GAGCACCATC ACGCACTCAG CTCACATGGA CATCTTCCAG
    AACCTGGCTG TGGATTTGGA CACTGAAGGT CGGTATCTCT TCTTGAATGC AATTGCAAAT
    CAGCTGCGGT ACCCAAATAG CCACACTCAC TACTTCAGCT GCACCATGCT GTACCTTTTT
    GCAGAGGCCA ACACTGAAGC TATTCAAGAA CAGATCACAA GGGTTCTTTT GGAACGGTTG
    ATTGTAAATA GGCCACATCC TTGGGGTCTT CTTATTACCT TCATTGAGCT GATTAAAAAT
    CCAGCATTTA AATTCTGGAA CCATGAATTT GTACACTGTG CCCCAGAAAT TGAAAAGTTA
    TTCCAGTCCG TCGCACAGTG CTGCATGGGA CAAAAGCAGG CTCAGCAAGT AATGGAAGGG
    ACAGGTGCCA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834140.1
    CDS328..7482
    Translation

    Target ORF information:

    RefSeq Version XM_024978372.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGACGGACTT GTCTCAGGTG
    TGGCCAGAGG CAAATCAGCA CTTTAGTAAA GAGATAGATG ATGAAGCAAA CAGCTATTTC
    CAGAGAATAT ATAATCACCC ACCACATCCA ACCATGTCTG TTGATGAGGT ATTAGAAATG
    CTACAGAGAT TTAAAGATTC TACCATAAAG AGGGAACGAG AAGTATTTAA CTGTATGCTA
    AGGAACTTGT TCGAAGAATA TCGGTTCTTC CCCCAATACC CTGATAAAGA GTTACATATA
    ACAGCCTGCC TGTTTGGTGG AATAATCGAA AAAGGACTGG TCACTTATAT GGCACTAGGT
    TTGGCCCTGC GATACGTTCT TGAAGCCTTA CGCAAGCCTT TTGGATCCAA AATGTATTAT
    TTTGGGATTG CTGCACTAGA TAGATTTAAA AATAGATTGA AGGACTATCC CCAGTATTGT
    CAGCACTTGG CTTCGATCAG TCACTTTATG CAATTTCCAC ATCATTTACA GGAGTATATT
    GAGTATGGAC AGCAATCTAG AGATCCTCCT GTGAAAATGC AGGGCTCTAT CACAACCCCT
    GGAAGTATTG CACTGGCTCA AGCCCAGGCC CAGGCCCAGG TTCCAGCAAA AGCTCCCCTT
    GCTGGCCAGG TTAGCACTAT GGTAACCACC TCAACAACCA CCACTGTTGC TAAAACGGTT
    ACAGTCACCA GGCCAACTGG AGTCAGCTTT AAGAAAGATG TGCCACCTTC TATTAATACA
    ACAAATATAG ATACATTGCT TGTGGCCACA GATCAAACTG AGAGAATCGT AGAGCCCCCA
    GAAAATATCC AGGAGAAAAT TGCTTTTATC TTCAACAACC TTTCCCAGTC AAATATGACA
    CAAAAGGTTG AAGAACTAAA GGAAACTGTG AAGGAAGAAT TTATGCCTTG GGTTTCACAG
    TACCTGGTTA TGAAGAGAGT CAGTATTGAG CCAAACTTTC ACAGCCTGTA TTCTAACTTC
    CTTGACACGC TGAAGAATCC TGAGTTTAAT AAGATGGTTC TGAATGAGAC CTATAGAAAC
    ATCAAAGTAC TCCTGACCTC TGATAAAGCT GCAGCCAATT TCTCTGATCG GTCTTTGCTG
    AAGAACTTGG GACATTGGCT AGGAATGATT ACACTAGCTA AAAATAAACC CATCTTACAC
    ACTGACTTGG ATGTAAAATC TTTGCTGCTA GAGGCTTATG TTAAAGGACA GCAAGAGTTG
    CTCTATGTAG TGCCCTTTGT TGCCAAAGTC TTAGAATCTA GCATTCGTAG TGTGGTTTTT
    AGGCCACCCA ACCCCTGGAC AATGGCTATT ATGAATGTAT TAGCTGAGCT ACATCAAGAA
    CATGACTTAA AGTTAAACTT GAAGTTTGAA ATTGAGGTCC TCTGTAAGAA TCTTGCATTA
    GACATCAATG AGCTAAAACC TGGAAACCTC CTAAAGGATA AAGATCGCCT GAAGAACTTA
    GATGAGCAAC TCTCTGCTCC AAAGAAAGAT GTCAAGCAGC CAGAGGAGCT CCCTCCTATC
    ACAACCACAA CAACTTCTAC AACACCAGCT ACCAGCACCA CCTGTACAGC CACTGTTCCA
    CCACAGCCAC AGTACAGTTA CCATGACATC AATGTCTATT CCCTCGCAGG CCTGGCACCA
    CACATTACTC TAAACCCAAC AATTCCCTTG TTTCAGGCCC ATCCACAGCT GAAGCAGTGT
    GTGCGCCAAG CAATCGAACG GGCTGTCCAG GAGCTGGTCC ATCCTGTGGT GGATCGATCA
    ATTAAGATTG CCATGACTAC TTGTGAGCAA ATAGTCAGGA AAGATTTTGC CCTGGATTCT
    GAGGAATCTC GAATGCGAAT AGCAGCTCAT CACATGATGC GGAACTTGAC AGCTGGAATG
    GCTATGATTA CTTGCAGGGA ACCTTTACTC ATGAGCATAT CAACCAACCT GAAAAACAGT
    TTTGCCTCAG CCCTTCGTAC TGCAAGCCCA CAACAAAGGG AAATGATGGA TCAGGCAGCT
    GCTCAATTAG CTCAGGACAA TTGTGAATTA GCTTGCTGTT TTATTCAGAA GACTGCAGTA
    GAAAAAGCAG GCCCTGAGAT GGACAAGAGA TTAGCAACTG AATTTGAGCT GAGAAAACAT
    GCTAGGCAAG AAGGGCGAAG GTATTGTGAT CCTGTTGTGT TAACATACCA AGCTGAGCGG
    ATGCCAGAGC AAATCAGGCT GAAAGTTGGT GGTGTGGACC CAAAGCAGCT GGCTGTTTAT
    GAAGAGTTTG CACGCAATGT GCCTGGCTTC TTGCCTACAA ATGATTTAAG TCAGCCCACA
    GGATTTTTAG CCCAGCCCAT GAAGCAAGCT TGGGCAACAG ATGATGTAGC TCAGATTTAT
    GATAAGTGTA TTACAGAACT GGAGCAGCAT CTTCACGCTA TCCCGCCAAC TTTGGCCATG
    AACCCCCAAG CTCAGGCCCT CAGAAGTCTT CTGGAGGTTG TAGTATTATC ACGGAACTCT
    CGAGATGCCA TTGCTGCTCT TGGATTGCTC CAGAAGGCTG TAGAGGGCTT ACTGGATGCC
    ACCAGTGGTG CTGATGCTGA CCTCCTTCTT CGCTACAGAG AGTGCCACCT CTTGGTCTTG
    AAAGCTCTTC AAGATGGCCG GGCATATGGG TCTCCATGGT GTAACAAACA GATCACAAGG
    TGCCTAATTG AGTGTCGGGA TGAATACAAA TATAATGTGG AGGCAGTGGA GTTGCTAATT
    CGCAATCATT TGGTCAACAT GCAACAGTAT GATCTTCACC TTGCTCAGTC AATGGAGAAT
    GGTTTAAACT ACATGGCTGT GGCATTTGCT ATGCAATTGG TAAAAATACT GCTGGTGGAT
    GAAAGGAGTG TTGCCCATGT TACTGAGGCA GATCTGTTCC ACACCATTGA AACCCTCATG
    AGGATTAATG CTCACTCCAG AGGCAATGCT CCTGAAGGAT TGCCCCAACT GATGGAAGTA
    GTGCGATCCA ACTATGAAGC AATGATTGAT CGTGCTCATG GAGGCCCTAA CTTTATGATG
    CATTCTGGGA TCTCTCAAGC CTCAGAGTAT GATGATCCTC CAGGCCTGAG GGAGAAGGCA
    GAGTATCTTC TGAGGGAATG GGTGAATCTC TACCATTCAG CAGCAGCTGG TCGTGACAGT
    ACCAAAGCTT TCTCTGCGTT TGTTGGACAG ATGCACCAGC AAGGAATCCT GAAGACTGAT
    GACCTCATAA CAAGGTTCTT TCGTCTGTGT ACTGAAATGT GTGTTGAAAT CAGTTACCGT
    GCTCAGGCAG AGCAGCAGCA CAATCCTGCT GCTAATCCCA CCATGATCCG AGCCAAGTGC
    TATCACAACC TGGATGCCTT TGTCCGACTC ATTGCACTGC TAGTAAAGCA CTCAGGGGAG
    GCCACCAACA CTGTCACAAA GATTAATCTG CTGAATAAGG TCCTCGGTAT AGTAGTGGGA
    GTTCTCCTAC AGGATCATGA TGTTCGGCAA AGTGAATTTC AGCAACTTCC CTACCACCGA
    ATTTTTATCA TGCTGCTCTT GGAACTCAAT GCACCTGAGC ATGTGTTGGA AACCATTAAT
    TTCCAGACAC TTACAGCTTT CTGCAACACA TTCCACATTT TGAGGCCTAC CAAAGCTCCT
    GGCTTTGTGT ATGCTTGGCT TGAACTGATC TCCCATCGGA TATTTATTGC AAGAATGCTG
    GCACACACGC CACAGCAGAA GGGATGGCCT ATGTATGCAC AGTTACTGAT TGATTTATTC
    AAATATTTAG CGCCTTTCCT TAGAAATGTG GAACTCACCA AACCTATGCA AATCCTCTAC
    AAGGGCACTT TAAGGGTGCT GCTGGTTCTT TTGCATGATT TTCCTGAGTT CCTTTGTGAT
    TACCATTATG GATTTTGTGA CGTGATCCCA CCTAATTGTA TCCAGCTAAG AAATCTGATC
    CTGAGTGCCT TCCCAAGAAA CATGAGGCTC CCAGACCCAT TCACTCCTAA TCTGAAGGTG
    GATATGTTGA GTGAAATTAA CATCGCTCCC CGGATTCTCA CCAATTTTAC TGGAGTGATG
    CCACCTCAAT TCAAAAAGGA TTTGGATTCC TATCTTAAAA CTCGATCACC TGTCACTTTC
    CTGTCTGATC TGCGCAGCAA CCTACAGGTA TCCAATGAGC CTGGTAATCG CTACAACCTC
    CAGCTCATTA ATGCACTGGT GCTCTATGTA GGGACTCAGG CCATTGCACA CATCCACAAC
    AAGGGCAGCA CACCTTCAAT GAGCACCATC ACGCACTCAG CTCACATGGA CATCTTCCAG
    AACCTGGCTG TGGATTTGGA CACTGAAGGT CGGTATCTCT TCTTGAATGC AATTGCAAAT
    CAGCTGCGGT ACCCAAATAG CCACACTCAC TACTTCAGCT GCACCATGCT GTACCTTTTT
    GCAGAGGCCA ACACTGAAGC TATTCAAGAA CAGATCACAA GGGTTCTTTT GGAACGGTTG
    ATTGTAAATA GGCCACATCC TTGGGGTCTT CTTATTACCT TCATTGAGCT GATTAAAAAT
    CCAGCATTTA AATTCTGGAA CCATGAATTT GTACACTGTG CCCCAGAAAT TGAAAAGTTA
    TTCCAGTCCG TCGCACAGTG CTGCATGGGA CAAAAGCAGG CTCAGCAAGT AATGGAAGGG
    ACAGGTGCCA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225093
    Clone ID Related Accession (Same CDS sequence) XM_024978373.1
    Accession Version XM_024978373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGTAAAGA GATAGATGAT
    GAAGCAAACA GCTATTTCCA GAGAATATAT AATCACCCAC CACATCCAAC CATGTCTGTT
    GATGAGGTAT TAGAAATGCT ACAGAGATTT AAAGATTCTA CCATAAAGAG GGAACGAGAA
    GTATTTAACT GTATGCTAAG GAACTTGTTC GAAGAATATC GGTTCTTCCC CCAATACCCT
    GATAAAGAGT TACATATAAC AGCCTGCCTG TTTGGTGGAA TAATCGAAAA AGGACTGGTC
    ACTTATATGG CACTAGGTTT GGCCCTGCGA TACGTTCTTG AAGCCTTACG CAAGCCTTTT
    GGATCCAAAA TGTATTATTT TGGGATTGCT GCACTAGATA GATTTAAAAA TAGATTGAAG
    GACTATCCCC AGTATTGTCA GCACTTGGCT TCGATCAGTC ACTTTATGCA ATTTCCACAT
    CATTTACAGG AGTATATTGA GTATGGACAG CAATCTAGAG ATCCTCCTGT GAAAATGCAG
    GGCTCTATCA CAACCCCTGG AAGTATTGCA CTGGCTCAAG CCCAGGCCCA GGCCCAGGTT
    CCAGCAAAAG CTCCCCTTGC TGGCCAGGTT AGCACTATGG TAACCACCTC AACAACCACC
    ACTGTTGCTA AAACGGTTAC AGTCACCAGG CCAACTGGAG TCAGCTTTAA GAAAGATGTG
    CCACCTTCTA TTAATACAAC AAATATAGAT ACATTGCTTG TGGCCACAGA TCAAACTGAG
    AGAATCGTAG AGCCCCCAGA AAATATCCAG GAGAAAATTG CTTTTATCTT CAACAACCTT
    TCCCAGTCAA ATATGACACA AAAGGTTGAA GAACTAAAGG AAACTGTGAA GGAAGAATTT
    ATGCCTTGGG TTTCACAGTA CCTGGTTATG AAGAGAGTCA GTATTGAGCC AAACTTTCAC
    AGCCTGTATT CTAACTTCCT TGACACGCTG AAGAATCCTG AGTTTAATAA GATGGTTCTG
    AATGAGACCT ATAGAAACAT CAAAGTACTC CTGACCTCTG ATAAAGCTGC AGCCAATTTC
    TCTGATCGGT CTTTGCTGAA GAACTTGGGA CATTGGCTAG GAATGATTAC ACTAGCTAAA
    AATAAACCCA TCTTACACAC TGACTTGGAT GTAAAATCTT TGCTGCTAGA GGCTTATGTT
    AAAGGACAGC AAGAGTTGCT CTATGTAGTG CCCTTTGTTG CCAAAGTCTT AGAATCTAGC
    ATTCGTAGTG TGGTTTTTAG GCCACCCAAC CCCTGGACAA TGGCTATTAT GAATGTATTA
    GCTGAGCTAC ATCAAGAACA TGACTTAAAG TTAAACTTGA AGTTTGAAAT TGAGGTCCTC
    TGTAAGAATC TTGCATTAGA CATCAATGAG CTAAAACCTG GAAACCTCCT AAAGGATAAA
    GATCGCCTGA AGAACTTAGA TGAGCAACTC TCTGCTCCAA AGAAAGATGT CAAGCAGCCA
    GAGGAGCTCC CTCCTATCAC AACCACAACA ACTTCTACAA CACCAGCTAC CAGCACCACC
    TGTACAGCCA CTGTTCCACC ACAGCCACAG TACAGTTACC ATGACATCAA TGTCTATTCC
    CTCGCAGGCC TGGCACCACA CATTACTCTA AACCCAACAA TTCCCTTGTT TCAGGCCCAT
    CCACAGCTGA AGCAGTGTGT GCGCCAAGCA ATCGAACGGG CTGTCCAGGA GCTGGTCCAT
    CCTGTGGTGG ATCGATCAAT TAAGATTGCC ATGACTACTT GTGAGCAAAT AGTCAGGAAA
    GATTTTGCCC TGGATTCTGA GGAATCTCGA ATGCGAATAG CAGCTCATCA CATGATGCGG
    AACTTGACAG CTGGAATGGC TATGATTACT TGCAGGGAAC CTTTACTCAT GAGCATATCA
    ACCAACCTGA AAAACAGTTT TGCCTCAGCC CTTCGTACTG CAAGCCCACA ACAAAGGGAA
    ATGATGGATC AGGCAGCTGC TCAATTAGCT CAGGACAATT GTGAATTAGC TTGCTGTTTT
    ATTCAGAAGA CTGCAGTAGA AAAAGCAGGC CCTGAGATGG ACAAGAGATT AGCAACTGAA
    TTTGAGCTGA GAAAACATGC TAGGCAAGAA GGGCGAAGGT ATTGTGATCC TGTTGTGTTA
    ACATACCAAG CTGAGCGGAT GCCAGAGCAA ATCAGGCTGA AAGTTGGTGG TGTGGACCCA
    AAGCAGCTGG CTGTTTATGA AGAGTTTGCA CGCAATGTGC CTGGCTTCTT GCCTACAAAT
    GATTTAAGTC AGCCCACAGG ATTTTTAGCC CAGCCCATGA AGCAAGCTTG GGCAACAGAT
    GATGTAGCTC AGATTTATGA TAAGTGTATT ACAGAACTGG AGCAGCATCT TCACGCTATC
    CCGCCAACTT TGGCCATGAA CCCCCAAGCT CAGGCCCTCA GAAGTCTTCT GGAGGTTGTA
    GTATTATCAC GGAACTCTCG AGATGCCATT GCTGCTCTTG GATTGCTCCA GAAGGCTGTA
    GAGGGCTTAC TGGATGCCAC CAGTGGTGCT GATGCTGACC TCCTTCTTCG CTACAGAGAG
    TGCCACCTCT TGGTCTTGAA AGCTCTTCAA GATGGCCGGG CATATGGGTC TCCATGGTGT
    AACAAACAGA TCACAAGGTG CCTAATTGAG TGTCGGGATG AATACAAATA TAATGTGGAG
    GCAGTGGAGT TGCTAATTCG CAATCATTTG GTCAACATGC AACAGTATGA TCTTCACCTT
    GCTCAGTCAA TGGAGAATGG TTTAAACTAC ATGGCTGTGG CATTTGCTAT GCAATTGGTA
    AAAATACTGC TGGTGGATGA AAGGAGTGTT GCCCATGTTA CTGAGGCAGA TCTGTTCCAC
    ACCATTGAAA CCCTCATGAG GATTAATGCT CACTCCAGAG GCAATGCTCC TGAAGGATTG
    CCCCAACTGA TGGAAGTAGT GCGATCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA
    GGCCCTAACT TTATGATGCA TTCTGGGATC TCTCAAGCCT CAGAGTATGA TGATCCTCCA
    GGCCTGAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCA
    GCAGCTGGTC GTGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGAT GCACCAGCAA
    GGAATCCTGA AGACTGATGA CCTCATAACA AGGTTCTTTC GTCTGTGTAC TGAAATGTGT
    GTTGAAATCA GTTACCGTGC TCAGGCAGAG CAGCAGCACA ATCCTGCTGC TAATCCCACC
    ATGATCCGAG CCAAGTGCTA TCACAACCTG GATGCCTTTG TCCGACTCAT TGCACTGCTA
    GTAAAGCACT CAGGGGAGGC CACCAACACT GTCACAAAGA TTAATCTGCT GAATAAGGTC
    CTCGGTATAG TAGTGGGAGT TCTCCTACAG GATCATGATG TTCGGCAAAG TGAATTTCAG
    CAACTTCCCT ACCACCGAAT TTTTATCATG CTGCTCTTGG AACTCAATGC ACCTGAGCAT
    GTGTTGGAAA CCATTAATTT CCAGACACTT ACAGCTTTCT GCAACACATT CCACATTTTG
    AGGCCTACCA AAGCTCCTGG CTTTGTGTAT GCTTGGCTTG AACTGATCTC CCATCGGATA
    TTTATTGCAA GAATGCTGGC ACACACGCCA CAGCAGAAGG GATGGCCTAT GTATGCACAG
    TTACTGATTG ATTTATTCAA ATATTTAGCG CCTTTCCTTA GAAATGTGGA ACTCACCAAA
    CCTATGCAAA TCCTCTACAA GGGCACTTTA AGGGTGCTGC TGGTTCTTTT GCATGATTTT
    CCTGAGTTCC TTTGTGATTA CCATTATGGA TTTTGTGACG TGATCCCACC TAATTGTATC
    CAGCTAAGAA ATCTGATCCT GAGTGCCTTC CCAAGAAACA TGAGGCTCCC AGACCCATTC
    ACTCCTAATC TGAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCCCG GATTCTCACC
    AATTTTACTG GAGTGATGCC ACCTCAATTC AAAAAGGATT TGGATTCCTA TCTTAAAACT
    CGATCACCTG TCACTTTCCT GTCTGATCTG CGCAGCAACC TACAGGTATC CAATGAGCCT
    GGTAATCGCT ACAACCTCCA GCTCATTAAT GCACTGGTGC TCTATGTAGG GACTCAGGCC
    ATTGCACACA TCCACAACAA GGGCAGCACA CCTTCAATGA GCACCATCAC GCACTCAGCT
    CACATGGACA TCTTCCAGAA CCTGGCTGTG GATTTGGACA CTGAAGGTCG GTATCTCTTC
    TTGAATGCAA TTGCAAATCA GCTGCGGTAC CCAAATAGCC ACACTCACTA CTTCAGCTGC
    ACCATGCTGT ACCTTTTTGC AGAGGCCAAC ACTGAAGCTA TTCAAGAACA GATCACAAGG
    GTTCTTTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTTCT TATTACCTTC
    ATTGAGCTGA TTAAAAATCC AGCATTTAAA TTCTGGAACC ATGAATTTGT ACACTGTGCC
    CCAGAAATTG AAAAGTTATT CCAGTCCGTC GCACAGTGCT GCATGGGACA AAAGCAGGCT
    CAGCAAGTAA TGGAAGGGAC AGGTGCCAGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834141.1
    CDS328..7440
    Translation

    Target ORF information:

    RefSeq Version XM_024978373.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    ACGGACTTGT CTCAGGTGTG GCCAGAGGCA AATCAGCACT TTAGTAAAGA GATAGATGAT
    GAAGCAAACA GCTATTTCCA GAGAATATAT AATCACCCAC CACATCCAAC CATGTCTGTT
    GATGAGGTAT TAGAAATGCT ACAGAGATTT AAAGATTCTA CCATAAAGAG GGAACGAGAA
    GTATTTAACT GTATGCTAAG GAACTTGTTC GAAGAATATC GGTTCTTCCC CCAATACCCT
    GATAAAGAGT TACATATAAC AGCCTGCCTG TTTGGTGGAA TAATCGAAAA AGGACTGGTC
    ACTTATATGG CACTAGGTTT GGCCCTGCGA TACGTTCTTG AAGCCTTACG CAAGCCTTTT
    GGATCCAAAA TGTATTATTT TGGGATTGCT GCACTAGATA GATTTAAAAA TAGATTGAAG
    GACTATCCCC AGTATTGTCA GCACTTGGCT TCGATCAGTC ACTTTATGCA ATTTCCACAT
    CATTTACAGG AGTATATTGA GTATGGACAG CAATCTAGAG ATCCTCCTGT GAAAATGCAG
    GGCTCTATCA CAACCCCTGG AAGTATTGCA CTGGCTCAAG CCCAGGCCCA GGCCCAGGTT
    CCAGCAAAAG CTCCCCTTGC TGGCCAGGTT AGCACTATGG TAACCACCTC AACAACCACC
    ACTGTTGCTA AAACGGTTAC AGTCACCAGG CCAACTGGAG TCAGCTTTAA GAAAGATGTG
    CCACCTTCTA TTAATACAAC AAATATAGAT ACATTGCTTG TGGCCACAGA TCAAACTGAG
    AGAATCGTAG AGCCCCCAGA AAATATCCAG GAGAAAATTG CTTTTATCTT CAACAACCTT
    TCCCAGTCAA ATATGACACA AAAGGTTGAA GAACTAAAGG AAACTGTGAA GGAAGAATTT
    ATGCCTTGGG TTTCACAGTA CCTGGTTATG AAGAGAGTCA GTATTGAGCC AAACTTTCAC
    AGCCTGTATT CTAACTTCCT TGACACGCTG AAGAATCCTG AGTTTAATAA GATGGTTCTG
    AATGAGACCT ATAGAAACAT CAAAGTACTC CTGACCTCTG ATAAAGCTGC AGCCAATTTC
    TCTGATCGGT CTTTGCTGAA GAACTTGGGA CATTGGCTAG GAATGATTAC ACTAGCTAAA
    AATAAACCCA TCTTACACAC TGACTTGGAT GTAAAATCTT TGCTGCTAGA GGCTTATGTT
    AAAGGACAGC AAGAGTTGCT CTATGTAGTG CCCTTTGTTG CCAAAGTCTT AGAATCTAGC
    ATTCGTAGTG TGGTTTTTAG GCCACCCAAC CCCTGGACAA TGGCTATTAT GAATGTATTA
    GCTGAGCTAC ATCAAGAACA TGACTTAAAG TTAAACTTGA AGTTTGAAAT TGAGGTCCTC
    TGTAAGAATC TTGCATTAGA CATCAATGAG CTAAAACCTG GAAACCTCCT AAAGGATAAA
    GATCGCCTGA AGAACTTAGA TGAGCAACTC TCTGCTCCAA AGAAAGATGT CAAGCAGCCA
    GAGGAGCTCC CTCCTATCAC AACCACAACA ACTTCTACAA CACCAGCTAC CAGCACCACC
    TGTACAGCCA CTGTTCCACC ACAGCCACAG TACAGTTACC ATGACATCAA TGTCTATTCC
    CTCGCAGGCC TGGCACCACA CATTACTCTA AACCCAACAA TTCCCTTGTT TCAGGCCCAT
    CCACAGCTGA AGCAGTGTGT GCGCCAAGCA ATCGAACGGG CTGTCCAGGA GCTGGTCCAT
    CCTGTGGTGG ATCGATCAAT TAAGATTGCC ATGACTACTT GTGAGCAAAT AGTCAGGAAA
    GATTTTGCCC TGGATTCTGA GGAATCTCGA ATGCGAATAG CAGCTCATCA CATGATGCGG
    AACTTGACAG CTGGAATGGC TATGATTACT TGCAGGGAAC CTTTACTCAT GAGCATATCA
    ACCAACCTGA AAAACAGTTT TGCCTCAGCC CTTCGTACTG CAAGCCCACA ACAAAGGGAA
    ATGATGGATC AGGCAGCTGC TCAATTAGCT CAGGACAATT GTGAATTAGC TTGCTGTTTT
    ATTCAGAAGA CTGCAGTAGA AAAAGCAGGC CCTGAGATGG ACAAGAGATT AGCAACTGAA
    TTTGAGCTGA GAAAACATGC TAGGCAAGAA GGGCGAAGGT ATTGTGATCC TGTTGTGTTA
    ACATACCAAG CTGAGCGGAT GCCAGAGCAA ATCAGGCTGA AAGTTGGTGG TGTGGACCCA
    AAGCAGCTGG CTGTTTATGA AGAGTTTGCA CGCAATGTGC CTGGCTTCTT GCCTACAAAT
    GATTTAAGTC AGCCCACAGG ATTTTTAGCC CAGCCCATGA AGCAAGCTTG GGCAACAGAT
    GATGTAGCTC AGATTTATGA TAAGTGTATT ACAGAACTGG AGCAGCATCT TCACGCTATC
    CCGCCAACTT TGGCCATGAA CCCCCAAGCT CAGGCCCTCA GAAGTCTTCT GGAGGTTGTA
    GTATTATCAC GGAACTCTCG AGATGCCATT GCTGCTCTTG GATTGCTCCA GAAGGCTGTA
    GAGGGCTTAC TGGATGCCAC CAGTGGTGCT GATGCTGACC TCCTTCTTCG CTACAGAGAG
    TGCCACCTCT TGGTCTTGAA AGCTCTTCAA GATGGCCGGG CATATGGGTC TCCATGGTGT
    AACAAACAGA TCACAAGGTG CCTAATTGAG TGTCGGGATG AATACAAATA TAATGTGGAG
    GCAGTGGAGT TGCTAATTCG CAATCATTTG GTCAACATGC AACAGTATGA TCTTCACCTT
    GCTCAGTCAA TGGAGAATGG TTTAAACTAC ATGGCTGTGG CATTTGCTAT GCAATTGGTA
    AAAATACTGC TGGTGGATGA AAGGAGTGTT GCCCATGTTA CTGAGGCAGA TCTGTTCCAC
    ACCATTGAAA CCCTCATGAG GATTAATGCT CACTCCAGAG GCAATGCTCC TGAAGGATTG
    CCCCAACTGA TGGAAGTAGT GCGATCCAAC TATGAAGCAA TGATTGATCG TGCTCATGGA
    GGCCCTAACT TTATGATGCA TTCTGGGATC TCTCAAGCCT CAGAGTATGA TGATCCTCCA
    GGCCTGAGGG AGAAGGCAGA GTATCTTCTG AGGGAATGGG TGAATCTCTA CCATTCAGCA
    GCAGCTGGTC GTGACAGTAC CAAAGCTTTC TCTGCGTTTG TTGGACAGAT GCACCAGCAA
    GGAATCCTGA AGACTGATGA CCTCATAACA AGGTTCTTTC GTCTGTGTAC TGAAATGTGT
    GTTGAAATCA GTTACCGTGC TCAGGCAGAG CAGCAGCACA ATCCTGCTGC TAATCCCACC
    ATGATCCGAG CCAAGTGCTA TCACAACCTG GATGCCTTTG TCCGACTCAT TGCACTGCTA
    GTAAAGCACT CAGGGGAGGC CACCAACACT GTCACAAAGA TTAATCTGCT GAATAAGGTC
    CTCGGTATAG TAGTGGGAGT TCTCCTACAG GATCATGATG TTCGGCAAAG TGAATTTCAG
    CAACTTCCCT ACCACCGAAT TTTTATCATG CTGCTCTTGG AACTCAATGC ACCTGAGCAT
    GTGTTGGAAA CCATTAATTT CCAGACACTT ACAGCTTTCT GCAACACATT CCACATTTTG
    AGGCCTACCA AAGCTCCTGG CTTTGTGTAT GCTTGGCTTG AACTGATCTC CCATCGGATA
    TTTATTGCAA GAATGCTGGC ACACACGCCA CAGCAGAAGG GATGGCCTAT GTATGCACAG
    TTACTGATTG ATTTATTCAA ATATTTAGCG CCTTTCCTTA GAAATGTGGA ACTCACCAAA
    CCTATGCAAA TCCTCTACAA GGGCACTTTA AGGGTGCTGC TGGTTCTTTT GCATGATTTT
    CCTGAGTTCC TTTGTGATTA CCATTATGGA TTTTGTGACG TGATCCCACC TAATTGTATC
    CAGCTAAGAA ATCTGATCCT GAGTGCCTTC CCAAGAAACA TGAGGCTCCC AGACCCATTC
    ACTCCTAATC TGAAGGTGGA TATGTTGAGT GAAATTAACA TCGCTCCCCG GATTCTCACC
    AATTTTACTG GAGTGATGCC ACCTCAATTC AAAAAGGATT TGGATTCCTA TCTTAAAACT
    CGATCACCTG TCACTTTCCT GTCTGATCTG CGCAGCAACC TACAGGTATC CAATGAGCCT
    GGTAATCGCT ACAACCTCCA GCTCATTAAT GCACTGGTGC TCTATGTAGG GACTCAGGCC
    ATTGCACACA TCCACAACAA GGGCAGCACA CCTTCAATGA GCACCATCAC GCACTCAGCT
    CACATGGACA TCTTCCAGAA CCTGGCTGTG GATTTGGACA CTGAAGGTCG GTATCTCTTC
    TTGAATGCAA TTGCAAATCA GCTGCGGTAC CCAAATAGCC ACACTCACTA CTTCAGCTGC
    ACCATGCTGT ACCTTTTTGC AGAGGCCAAC ACTGAAGCTA TTCAAGAACA GATCACAAGG
    GTTCTTTTGG AACGGTTGAT TGTAAATAGG CCACATCCTT GGGGTCTTCT TATTACCTTC
    ATTGAGCTGA TTAAAAATCC AGCATTTAAA TTCTGGAACC ATGAATTTGT ACACTGTGCC
    CCAGAAATTG AAAAGTTATT CCAGTCCGTC GCACAGTGCT GCATGGGACA AAAGCAGGCT
    CAGCAAGTAA TGGAAGGGAC AGGTGCCAGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225094
    Clone ID Related Accession (Same CDS sequence) XM_024978374.1
    Accession Version XM_024978374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATACTCAGTT TCTGATTCAG
    GAGTGTGCGT CACTGATTAC GAAGCCAAAT TTTATCTCGA CGCTGTCCTA TGCCATTGAT
    AATCCATTGC ACTATCAGAA GAGTTTAAAG CCTGCACCCC ACTTATTTGC CCAGCTGAGT
    AAAGTTCTCA AGTTAAGCAA AGTACAAGAG GTAATTTTTG GCCTTGCCCT CTTGAATTCT
    TCCAGCACAG ATCTCAGAGG TTTTGCTGCC CAGTTTATCA AACAGAAGCT TCCAGATCTT
    CTGCGTTCTT ACATTGACGC AGACGTCAGT GGAAATCAGG AAGGTGGCTT CCAAGATATA
    GCAATAGAGG TCCTACACCT CCTCCTGTCC CATCTCCTCT TTGGGCAGAA GGGAGCCTTT
    GGAGTTGGAC AGGAACAGAT AGACGCTTTT CTTAAGACGC TGCGCAGAGA TTTTCCCCAG
    GAACGCTGTC CTGTGGTGCT TGCACCACTT TTATACCCTG AAAAACGGGA CATTCTAATG
    GACAGAATCC TGCCTGATTC CGGAGGGGTA GCTAAAACCA TGATGGAGAG CTCTTTGGCT
    GATTTTATGC AAGAAGTGGG CTATGGCTTT TGTGCAAGTA TTGAAGAATG TCGCAATATA
    ATCATGCAGT TTGGTGTTCG GGAGGTCACA GCTGCCCAGG TTGCAAGGGT TTTAGGAATG
    ATGGCTCGAA CTCATTCAGG ATTAACAGAC GGAATTCCAT TACAGAGTAT CTCTGCTCCT
    GGCAGTGGAA TCTGGAGTGA TGGGAAAGAT AAAAGTGATG GAGCACAGGC ACACACATGG
    AATGTAGAAG TCTTGATCGA TGTTCTCAAA GAACTGAACC CGAGTTTGAA TTTCAAGGAA
    GTAACTTATG AACTGGACCA TCCTGGGTTT CAAATTCGTG ATAGTAAAGG ACTTCATAAT
    GTAGTCTATG GCATTCAAAG GGGTCTGGGT ATGGAAGTGT TCCCTGTAGA TCTCATATAC
    AGACCTTGGA AACACGCAGA AGGCCAGCTC TCCTTCATTC AGCATTCCCT TATAAATCCA
    GAGATCTTCT GTTTTGCCGA CTATCCCTGT CATACTGTTG CCACTGATAT TCTGAAAGCA
    CCACCAGAGG ATGACAATCG AGAAATTGCC ACATGGAAGA GCTTGGATTT GATTGAATCT
    CTGCTAAGGC TCGCAGAGGT AGGGCAATAT GAGCAAGTCA AACAGCTCTT CAGCTTCCCT
    ATCAAACACT GTCCAGACAT GCTGGTATTG GCCTTATTAC AAATCAACAC CTCCTGGCAC
    ACCTTGCGCC ACGAACTCAT CTCCACTCTG ATGCCAATTT TCCTTGGAAA CCACCCTAAC
    TCAGCTATAA TTTTGCACTA TGCATGGCAT GGACAGGGAC AGTCTCCCTC AATCCGCCAA
    CTTATCATGC ATGCAATGGC AGAATGGTAC ATGCGAGGGG AGCAGTATGA CCAGGCCAAG
    TTGTCTCGAA TACTTGATGT GGCCCAGGAC TTGAAGGTTT ATTTGAAAAA ACACAGGGTA
    AAGTTTATTG TGAAGGCTTT GTCAATGCTG CTAAATGGTA CTCCATTTGC CTTTGTTATT
    GACCTTGCTG CACTTGCTTC ACGTCGTGAA TACCTCAAAC TTGACAAATG GCTCACAGAT
    AAAATTCGAG AGCATGGAGA GCCTTTTATC CAGGCTTGTA TGACTTTTTT AAAGAGACGG
    TGTCCTTCTA TTTTGGGGGG ACTTGCCCCA GAGAAAGATC AGCCTAAAAG TGCTCAACTT
    CCTCCAGAAA CCCTCGCGAC AATGTTGGCC TGTTTACAAG CTTGTGCAGG GAGTGTTTCT
    CAGGAGTTAT CAGAAACCAT CCTCACCATG GTGGCCAATT GCAGTAACGT TATGAACAAG
    GCCAGGCAGC CACCACCGGG AGTTATGCCA AAAGGACGCC CTCCTAGTGC TAGCAGCTTG
    GACGCCATTT CTCCCGTTCA GATTGACCCT CTTGCTGGAA TGGCATCTCT TAGTATAGGT
    GGTTCAGCTG CCCCTCACAC CCAGAGTATG CAGGGCTTTC CTCCAAATTT GGGTTCTGCA
    TTCAGTACCC CACAGTCACC AGCAAAAGCA TTTCCACCCC TTTCAACCCC AAATCAGACA
    ACTGCATTCA GTGGTATTGG AGGACTTTCA TCACAGCTTC CAGTAGGTGG TCTTGGCACA
    GGCAGCCTGA CTGGTATAGG AACTGGGGCT CTTGGACTCC CTGCAGTGAA TAGCGATCCT
    TTTGTACAGA GGAAACTGGG CACCTCTGGA CTGAACCAGC CTACATTCCA GCAGAGTAAG
    ATGAAACCTT CGGACTTGTC TCAGGTGTGG CCAGAGGCAA ATCAGCACTT TAGTAAAGAG
    ATAGATGATG AAGCAAACAG CTATTTCCAG AGAATATATA ATCACCCACC ACATCCAACC
    ATGTCTGTTG ATGAGGTATT AGAAATGCTA CAGAGATTTA AAGATTCTAC CATAAAGAGG
    GAACGAGAAG TATTTAACTG TATGCTAAGG AACTTGTTCG AAGAATATCG GTTCTTCCCC
    CAATACCCTG ATAAAGAGTT ACATATAACA GCCTGCCTGT TTGGTGGAAT AATCGAAAAA
    GGACTGGTCA CTTATATGGC ACTAGGTTTG GCCCTGCGAT ACGTTCTTGA AGCCTTACGC
    AAGCCTTTTG GATCCAAAAT GTATTATTTT GGGATTGCTG CACTAGATAG ATTTAAAAAT
    AGATTGAAGG ACTATCCCCA GTATTGTCAG CACTTGGCTT CGATCAGTCA CTTTATGCAA
    TTTCCACATC ATTTACAGGA GTATATTGAG TATGGACAGC AATCTAGAGA TCCTCCTGTG
    AAAATGCAGG GCTCTATCAC AACCCCTGGA AGTATTGCAC TGGCTCAAGC CCAGGCCCAG
    GCCCAGGTTC CAGCAAAAGC TCCCCTTGCT GGCCAGGTTA GCACTATGGT AACCACCTCA
    ACAACCACCA CTGTTGCTAA AACGGTTACA GTCACCAGGC CAACTGGAGT CAGCTTTAAG
    AAAGATGTGC CACCTTCTAT TAATACAACA AATATAGATA CATTGCTTGT GGCCACAGAT
    CAAACTGAGA GAATCGTAGA GCCCCCAGAA AATATCCAGG AGAAAATTGC TTTTATCTTC
    AACAACCTTT CCCAGTCAAA TATGACACAA AAGGTTGAAG AACTAAAGGA AACTGTGAAG
    GAAGAATTTA TGCCTTGGGT TTCACAGTAC CTGGTTATGA AGAGAGTCAG TATTGAGCCA
    AACTTTCACA GCCTGTATTC TAACTTCCTT GACACGCTGA AGAATCCTGA GTTTAATAAG
    ATGGTTCTGA ATGAGACCTA TAGAAACATC AAAGTACTCC TGACCTCTGA TAAAGCTGCA
    GCCAATTTCT CTGATCGGTC TTTGCTGAAG AACTTGGGAC ATTGGCTAGG AATGATTACA
    CTAGCTAAAA ATAAACCCAT CTTACACACT GACTTGGATG TAAAATCTTT GCTGCTAGAG
    GCTTATGTTA AAGGACAGCA AGAGTTGCTC TATGTAGTGC CCTTTGTTGC CAAAGTCTTA
    GAATCTAGCA TTCGTAGTGT GGTTTTTAGG CCACCCAACC CCTGGACAAT GGCTATTATG
    AATGTATTAG CTGAGCTACA TCAAGAACAT GACTTAAAGT TAAACTTGAA GTTTGAAATT
    GAGGTCCTCT GTAAGAATCT TGCATTAGAC ATCAATGAGC TAAAACCTGG AAACCTCCTA
    AAGGATAAAG ATCGCCTGAA GAACTTAGAT GAGCAACTCT CTGCTCCAAA GAAAGATGTC
    AAGCAGCCAG AGGAGCTCCC TCCTATCACA ACCACAACAA CTTCTACAAC ACCAGCTACC
    AGCACCACCT GTACAGCCAC TGTTCCACCA CAGCCACAGT ACAGTTACCA TGACATCAAT
    GTCTATTCCC TCGCAGGCCT GGCACCACAC ATTACTCTAA ACCCAACAAT TCCCTTGTTT
    CAGGCCCATC CACAGCTGAA GCAGTGTGTG CGCCAAGCAA TCGAACGGGC TGTCCAGGAG
    CTGGTCCATC CTGTGGTGGA TCGATCAATT AAGATTGCCA TGACTACTTG TGAGCAAATA
    GTCAGGAAAG ATTTTGCCCT GGATTCTGAG GAATCTCGAA TGCGAATAGC AGCTCATCAC
    ATGATGCGGA ACTTGACAGC TGGAATGGCT ATGATTACTT GCAGGGAACC TTTACTCATG
    AGCATATCAA CCAACCTGAA AAACAGTTTT GCCTCAGCCC TTCGTACTGC AAGCCCACAA
    CAAAGGGAAA TGATGGATCA GGCAGCTGCT CAATTAGCTC AGGACAATTG TGAATTAGCT
    TGCTGTTTTA TTCAGAAGAC TGCAGTAGAA AAAGCAGGCC CTGAGATGGA CAAGAGATTA
    GCAACTGAAT TTGAGCTGAG AAAACATGCT AGGCAAGAAG GGCGAAGGTA TTGTGATCCT
    GTTGTGTTAA CATACCAAGC TGAGCGGATG CCAGAGCAAA TCAGGCTGAA AGTTGGTGGT
    GTGGACCCAA AGCAGCTGGC TGTTTATGAA GAGTTTGCAC GCAATGTGCC TGGCTTCTTG
    CCTACAAATG ATTTAAGTCA GCCCACAGGA TTTTTAGCCC AGCCCATGAA GCAAGCTTGG
    GCAACAGATG ATGTAGCTCA GATTTATGAT AAGTGTATTA CAGAACTGGA GCAGCATCTT
    CACGCTATCC CGCCAACTTT GGCCATGAAC CCCCAAGCTC AGGCCCTCAG AAGTCTTCTG
    GAGGTTGTAG TATTATCACG GAACTCTCGA GATGCCATTG CTGCTCTTGG ATTGCTCCAG
    AAGGCTGTAG AGGGCTTACT GGATGCCACC AGTGGTGCTG ATGCTGACCT CCTTCTTCGC
    TACAGAGAGT GCCACCTCTT GGTCTTGAAA GCTCTTCAAG ATGGCCGGGC ATATGGGTCT
    CCATGGTGTA ACAAACAGAT CACAAGGTGC CTAATTGAGT GTCGGGATGA ATACAAATAT
    AATGTGGAGG CAGTGGAGTT GCTAATTCGC AATCATTTGG TCAACATGCA ACAGTATGAT
    CTTCACCTTG CTCAGTCAAT GGAGAATGGT TTAAACTACA TGGCTGTGGC ATTTGCTATG
    CAATTGGTAA AAATACTGCT GGTGGATGAA AGGAGTGTTG CCCATGTTAC TGAGGCAGAT
    CTGTTCCACA CCATTGAAAC CCTCATGAGG ATTAATGCTC ACTCCAGAGG CAATGCTCCT
    GAAGGATTGC CCCAACTGAT GGAAGTAGTG CGATCCAACT ATGAAGCAAT GATTGATCGT
    GCTCATGGAG GCCCTAACTT TATGATGCAT TCTGGGATCT CTCAAGCCTC AGAGTATGAT
    GATCCTCCAG GCCTGAGGGA GAAGGCAGAG TATCTTCTGA GGGAATGGGT GAATCTCTAC
    CATTCAGCAG CAGCTGGTCG TGACAGTACC AAAGCTTTCT CTGCGTTTGT TGGACAGGTA
    GAGCTTTTGG AAAGAAAGAT GCACCAGCAA GGAATCCTGA AGACTGATGA CCTCATAACA
    AGGTTCTTTC GTCTGTGTAC TGAAATGTGT GTTGAAATCA GTTACCGTGC TCAGGCAGAG
    CAGCAGCACA ATCCTGCTGC TAATCCCACC ATGATCCGAG CCAAGTGCTA TCACAACCTG
    GATGCCTTTG TCCGACTCAT TGCACTGCTA GTAAAGCACT CAGGGGAGGC CACCAACACT
    GTCACAAAGA TTAATCTGCT GAATAAGGTC CTCGGTATAG TAGTGGGAGT TCTCCTACAG
    GATCATGATG TTCGGCAAAG TGAATTTCAG CAACTTCCCT ACCACCGAAT TTTTATCATG
    CTGCTCTTGG AACTCAATGC ACCTGAGCAT GTGTTGGAAA CCATTAATTT CCAGACACTT
    ACAGCTTTCT GCAACACATT CCACATTTTG AGGCCTACCA AAGCTCCTGG CTTTGTGTAT
    GCTTGGCTTG AACTGATCTC CCATCGGATA TTTATTGCAA GAATGCTGGC ACACACGCCA
    CAGCAGAAGG GATGGCCTAT GTATGCACAG TTACTGATTG ATTTATTCAA ATATTTAGCG
    CCTTTCCTTA GAAATGTGGA ACTCACCAAA CCTATGCAAA TCCTCTACAA GGGCACTTTA
    AGGGTGCTGC TGGTTCTTTT GCATGATTTT CCTGAGTTCC TTTGTGATTA CCATTATGGA
    TTTTGTGACG TGATCCCACC TAATTGTATC CAGCTAAGAA ATCTGATCCT GAGTGCCTTC
    CCAAGAAACA TGAGGCTCCC AGACCCATTC ACTCCTAATC TGAAGGTGGA TATGTTGAGT
    GAAATTAACA TCGCTCCCCG GATTCTCACC AATTTTACTG GAGTGATGCC ACCTCAATTC
    AAAAAGGATT TGGATTCCTA TCTTAAAACT CGATCACCTG TCACTTTCCT GTCTGATCTG
    CGCAGCAACC TACAGGTATC CAATGAGCCT GGTAATCGCT ACAACCTCCA GCTCATTAAT
    GCACTGGTGC TCTATGTAGG GACTCAGGCC ATTGCACACA TCCACAACAA GGGCAGCACA
    CCTTCAATGA GCACCATCAC GCACTCAGCT CACATGGACA TCTTCCAGAA CCTGGCTGTG
    GATTTGGACA CTGAAGGTCG GTATCTCTTC TTGAATGCAA TTGCAAATCA GCTGCGGTAC
    CCAAATAGCC ACACTCACTA CTTCAGCTGC ACCATGCTGT ACCTTTTTGC AGAGGCCAAC
    ACTGAAGCTA TTCAAGAACA GATCACAAGG GTTCTTTTGG AACGGTTGAT TGTAAATAGG
    CCACATCCTT GGGGTCTTCT TATTACCTTC ATTGAGCTGA TTAAAAATCC AGCATTTAAA
    TTCTGGAACC ATGAATTTGT ACACTGTGCC CCAGAAATTG AAAAGTTATT CCAGTCCGTC
    GCACAGTGCT GCATGGGACA AAAGCAGGCT CAGCAAGTAA TGGAAGGGAC AGGTGCCAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834142.1
    CDS328..7410
    Translation

    Target ORF information:

    RefSeq Version XM_024978374.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATACTCAGTT TCTGATTCAG
    GAGTGTGCGT CACTGATTAC GAAGCCAAAT TTTATCTCGA CGCTGTCCTA TGCCATTGAT
    AATCCATTGC ACTATCAGAA GAGTTTAAAG CCTGCACCCC ACTTATTTGC CCAGCTGAGT
    AAAGTTCTCA AGTTAAGCAA AGTACAAGAG GTAATTTTTG GCCTTGCCCT CTTGAATTCT
    TCCAGCACAG ATCTCAGAGG TTTTGCTGCC CAGTTTATCA AACAGAAGCT TCCAGATCTT
    CTGCGTTCTT ACATTGACGC AGACGTCAGT GGAAATCAGG AAGGTGGCTT CCAAGATATA
    GCAATAGAGG TCCTACACCT CCTCCTGTCC CATCTCCTCT TTGGGCAGAA GGGAGCCTTT
    GGAGTTGGAC AGGAACAGAT AGACGCTTTT CTTAAGACGC TGCGCAGAGA TTTTCCCCAG
    GAACGCTGTC CTGTGGTGCT TGCACCACTT TTATACCCTG AAAAACGGGA CATTCTAATG
    GACAGAATCC TGCCTGATTC CGGAGGGGTA GCTAAAACCA TGATGGAGAG CTCTTTGGCT
    GATTTTATGC AAGAAGTGGG CTATGGCTTT TGTGCAAGTA TTGAAGAATG TCGCAATATA
    ATCATGCAGT TTGGTGTTCG GGAGGTCACA GCTGCCCAGG TTGCAAGGGT TTTAGGAATG
    ATGGCTCGAA CTCATTCAGG ATTAACAGAC GGAATTCCAT TACAGAGTAT CTCTGCTCCT
    GGCAGTGGAA TCTGGAGTGA TGGGAAAGAT AAAAGTGATG GAGCACAGGC ACACACATGG
    AATGTAGAAG TCTTGATCGA TGTTCTCAAA GAACTGAACC CGAGTTTGAA TTTCAAGGAA
    GTAACTTATG AACTGGACCA TCCTGGGTTT CAAATTCGTG ATAGTAAAGG ACTTCATAAT
    GTAGTCTATG GCATTCAAAG GGGTCTGGGT ATGGAAGTGT TCCCTGTAGA TCTCATATAC
    AGACCTTGGA AACACGCAGA AGGCCAGCTC TCCTTCATTC AGCATTCCCT TATAAATCCA
    GAGATCTTCT GTTTTGCCGA CTATCCCTGT CATACTGTTG CCACTGATAT TCTGAAAGCA
    CCACCAGAGG ATGACAATCG AGAAATTGCC ACATGGAAGA GCTTGGATTT GATTGAATCT
    CTGCTAAGGC TCGCAGAGGT AGGGCAATAT GAGCAAGTCA AACAGCTCTT CAGCTTCCCT
    ATCAAACACT GTCCAGACAT GCTGGTATTG GCCTTATTAC AAATCAACAC CTCCTGGCAC
    ACCTTGCGCC ACGAACTCAT CTCCACTCTG ATGCCAATTT TCCTTGGAAA CCACCCTAAC
    TCAGCTATAA TTTTGCACTA TGCATGGCAT GGACAGGGAC AGTCTCCCTC AATCCGCCAA
    CTTATCATGC ATGCAATGGC AGAATGGTAC ATGCGAGGGG AGCAGTATGA CCAGGCCAAG
    TTGTCTCGAA TACTTGATGT GGCCCAGGAC TTGAAGGTTT ATTTGAAAAA ACACAGGGTA
    AAGTTTATTG TGAAGGCTTT GTCAATGCTG CTAAATGGTA CTCCATTTGC CTTTGTTATT
    GACCTTGCTG CACTTGCTTC ACGTCGTGAA TACCTCAAAC TTGACAAATG GCTCACAGAT
    AAAATTCGAG AGCATGGAGA GCCTTTTATC CAGGCTTGTA TGACTTTTTT AAAGAGACGG
    TGTCCTTCTA TTTTGGGGGG ACTTGCCCCA GAGAAAGATC AGCCTAAAAG TGCTCAACTT
    CCTCCAGAAA CCCTCGCGAC AATGTTGGCC TGTTTACAAG CTTGTGCAGG GAGTGTTTCT
    CAGGAGTTAT CAGAAACCAT CCTCACCATG GTGGCCAATT GCAGTAACGT TATGAACAAG
    GCCAGGCAGC CACCACCGGG AGTTATGCCA AAAGGACGCC CTCCTAGTGC TAGCAGCTTG
    GACGCCATTT CTCCCGTTCA GATTGACCCT CTTGCTGGAA TGGCATCTCT TAGTATAGGT
    GGTTCAGCTG CCCCTCACAC CCAGAGTATG CAGGGCTTTC CTCCAAATTT GGGTTCTGCA
    TTCAGTACCC CACAGTCACC AGCAAAAGCA TTTCCACCCC TTTCAACCCC AAATCAGACA
    ACTGCATTCA GTGGTATTGG AGGACTTTCA TCACAGCTTC CAGTAGGTGG TCTTGGCACA
    GGCAGCCTGA CTGGTATAGG AACTGGGGCT CTTGGACTCC CTGCAGTGAA TAGCGATCCT
    TTTGTACAGA GGAAACTGGG CACCTCTGGA CTGAACCAGC CTACATTCCA GCAGAGTAAG
    ATGAAACCTT CGGACTTGTC TCAGGTGTGG CCAGAGGCAA ATCAGCACTT TAGTAAAGAG
    ATAGATGATG AAGCAAACAG CTATTTCCAG AGAATATATA ATCACCCACC ACATCCAACC
    ATGTCTGTTG ATGAGGTATT AGAAATGCTA CAGAGATTTA AAGATTCTAC CATAAAGAGG
    GAACGAGAAG TATTTAACTG TATGCTAAGG AACTTGTTCG AAGAATATCG GTTCTTCCCC
    CAATACCCTG ATAAAGAGTT ACATATAACA GCCTGCCTGT TTGGTGGAAT AATCGAAAAA
    GGACTGGTCA CTTATATGGC ACTAGGTTTG GCCCTGCGAT ACGTTCTTGA AGCCTTACGC
    AAGCCTTTTG GATCCAAAAT GTATTATTTT GGGATTGCTG CACTAGATAG ATTTAAAAAT
    AGATTGAAGG ACTATCCCCA GTATTGTCAG CACTTGGCTT CGATCAGTCA CTTTATGCAA
    TTTCCACATC ATTTACAGGA GTATATTGAG TATGGACAGC AATCTAGAGA TCCTCCTGTG
    AAAATGCAGG GCTCTATCAC AACCCCTGGA AGTATTGCAC TGGCTCAAGC CCAGGCCCAG
    GCCCAGGTTC CAGCAAAAGC TCCCCTTGCT GGCCAGGTTA GCACTATGGT AACCACCTCA
    ACAACCACCA CTGTTGCTAA AACGGTTACA GTCACCAGGC CAACTGGAGT CAGCTTTAAG
    AAAGATGTGC CACCTTCTAT TAATACAACA AATATAGATA CATTGCTTGT GGCCACAGAT
    CAAACTGAGA GAATCGTAGA GCCCCCAGAA AATATCCAGG AGAAAATTGC TTTTATCTTC
    AACAACCTTT CCCAGTCAAA TATGACACAA AAGGTTGAAG AACTAAAGGA AACTGTGAAG
    GAAGAATTTA TGCCTTGGGT TTCACAGTAC CTGGTTATGA AGAGAGTCAG TATTGAGCCA
    AACTTTCACA GCCTGTATTC TAACTTCCTT GACACGCTGA AGAATCCTGA GTTTAATAAG
    ATGGTTCTGA ATGAGACCTA TAGAAACATC AAAGTACTCC TGACCTCTGA TAAAGCTGCA
    GCCAATTTCT CTGATCGGTC TTTGCTGAAG AACTTGGGAC ATTGGCTAGG AATGATTACA
    CTAGCTAAAA ATAAACCCAT CTTACACACT GACTTGGATG TAAAATCTTT GCTGCTAGAG
    GCTTATGTTA AAGGACAGCA AGAGTTGCTC TATGTAGTGC CCTTTGTTGC CAAAGTCTTA
    GAATCTAGCA TTCGTAGTGT GGTTTTTAGG CCACCCAACC CCTGGACAAT GGCTATTATG
    AATGTATTAG CTGAGCTACA TCAAGAACAT GACTTAAAGT TAAACTTGAA GTTTGAAATT
    GAGGTCCTCT GTAAGAATCT TGCATTAGAC ATCAATGAGC TAAAACCTGG AAACCTCCTA
    AAGGATAAAG ATCGCCTGAA GAACTTAGAT GAGCAACTCT CTGCTCCAAA GAAAGATGTC
    AAGCAGCCAG AGGAGCTCCC TCCTATCACA ACCACAACAA CTTCTACAAC ACCAGCTACC
    AGCACCACCT GTACAGCCAC TGTTCCACCA CAGCCACAGT ACAGTTACCA TGACATCAAT
    GTCTATTCCC TCGCAGGCCT GGCACCACAC ATTACTCTAA ACCCAACAAT TCCCTTGTTT
    CAGGCCCATC CACAGCTGAA GCAGTGTGTG CGCCAAGCAA TCGAACGGGC TGTCCAGGAG
    CTGGTCCATC CTGTGGTGGA TCGATCAATT AAGATTGCCA TGACTACTTG TGAGCAAATA
    GTCAGGAAAG ATTTTGCCCT GGATTCTGAG GAATCTCGAA TGCGAATAGC AGCTCATCAC
    ATGATGCGGA ACTTGACAGC TGGAATGGCT ATGATTACTT GCAGGGAACC TTTACTCATG
    AGCATATCAA CCAACCTGAA AAACAGTTTT GCCTCAGCCC TTCGTACTGC AAGCCCACAA
    CAAAGGGAAA TGATGGATCA GGCAGCTGCT CAATTAGCTC AGGACAATTG TGAATTAGCT
    TGCTGTTTTA TTCAGAAGAC TGCAGTAGAA AAAGCAGGCC CTGAGATGGA CAAGAGATTA
    GCAACTGAAT TTGAGCTGAG AAAACATGCT AGGCAAGAAG GGCGAAGGTA TTGTGATCCT
    GTTGTGTTAA CATACCAAGC TGAGCGGATG CCAGAGCAAA TCAGGCTGAA AGTTGGTGGT
    GTGGACCCAA AGCAGCTGGC TGTTTATGAA GAGTTTGCAC GCAATGTGCC TGGCTTCTTG
    CCTACAAATG ATTTAAGTCA GCCCACAGGA TTTTTAGCCC AGCCCATGAA GCAAGCTTGG
    GCAACAGATG ATGTAGCTCA GATTTATGAT AAGTGTATTA CAGAACTGGA GCAGCATCTT
    CACGCTATCC CGCCAACTTT GGCCATGAAC CCCCAAGCTC AGGCCCTCAG AAGTCTTCTG
    GAGGTTGTAG TATTATCACG GAACTCTCGA GATGCCATTG CTGCTCTTGG ATTGCTCCAG
    AAGGCTGTAG AGGGCTTACT GGATGCCACC AGTGGTGCTG ATGCTGACCT CCTTCTTCGC
    TACAGAGAGT GCCACCTCTT GGTCTTGAAA GCTCTTCAAG ATGGCCGGGC ATATGGGTCT
    CCATGGTGTA ACAAACAGAT CACAAGGTGC CTAATTGAGT GTCGGGATGA ATACAAATAT
    AATGTGGAGG CAGTGGAGTT GCTAATTCGC AATCATTTGG TCAACATGCA ACAGTATGAT
    CTTCACCTTG CTCAGTCAAT GGAGAATGGT TTAAACTACA TGGCTGTGGC ATTTGCTATG
    CAATTGGTAA AAATACTGCT GGTGGATGAA AGGAGTGTTG CCCATGTTAC TGAGGCAGAT
    CTGTTCCACA CCATTGAAAC CCTCATGAGG ATTAATGCTC ACTCCAGAGG CAATGCTCCT
    GAAGGATTGC CCCAACTGAT GGAAGTAGTG CGATCCAACT ATGAAGCAAT GATTGATCGT
    GCTCATGGAG GCCCTAACTT TATGATGCAT TCTGGGATCT CTCAAGCCTC AGAGTATGAT
    GATCCTCCAG GCCTGAGGGA GAAGGCAGAG TATCTTCTGA GGGAATGGGT GAATCTCTAC
    CATTCAGCAG CAGCTGGTCG TGACAGTACC AAAGCTTTCT CTGCGTTTGT TGGACAGGTA
    GAGCTTTTGG AAAGAAAGAT GCACCAGCAA GGAATCCTGA AGACTGATGA CCTCATAACA
    AGGTTCTTTC GTCTGTGTAC TGAAATGTGT GTTGAAATCA GTTACCGTGC TCAGGCAGAG
    CAGCAGCACA ATCCTGCTGC TAATCCCACC ATGATCCGAG CCAAGTGCTA TCACAACCTG
    GATGCCTTTG TCCGACTCAT TGCACTGCTA GTAAAGCACT CAGGGGAGGC CACCAACACT
    GTCACAAAGA TTAATCTGCT GAATAAGGTC CTCGGTATAG TAGTGGGAGT TCTCCTACAG
    GATCATGATG TTCGGCAAAG TGAATTTCAG CAACTTCCCT ACCACCGAAT TTTTATCATG
    CTGCTCTTGG AACTCAATGC ACCTGAGCAT GTGTTGGAAA CCATTAATTT CCAGACACTT
    ACAGCTTTCT GCAACACATT CCACATTTTG AGGCCTACCA AAGCTCCTGG CTTTGTGTAT
    GCTTGGCTTG AACTGATCTC CCATCGGATA TTTATTGCAA GAATGCTGGC ACACACGCCA
    CAGCAGAAGG GATGGCCTAT GTATGCACAG TTACTGATTG ATTTATTCAA ATATTTAGCG
    CCTTTCCTTA GAAATGTGGA ACTCACCAAA CCTATGCAAA TCCTCTACAA GGGCACTTTA
    AGGGTGCTGC TGGTTCTTTT GCATGATTTT CCTGAGTTCC TTTGTGATTA CCATTATGGA
    TTTTGTGACG TGATCCCACC TAATTGTATC CAGCTAAGAA ATCTGATCCT GAGTGCCTTC
    CCAAGAAACA TGAGGCTCCC AGACCCATTC ACTCCTAATC TGAAGGTGGA TATGTTGAGT
    GAAATTAACA TCGCTCCCCG GATTCTCACC AATTTTACTG GAGTGATGCC ACCTCAATTC
    AAAAAGGATT TGGATTCCTA TCTTAAAACT CGATCACCTG TCACTTTCCT GTCTGATCTG
    CGCAGCAACC TACAGGTATC CAATGAGCCT GGTAATCGCT ACAACCTCCA GCTCATTAAT
    GCACTGGTGC TCTATGTAGG GACTCAGGCC ATTGCACACA TCCACAACAA GGGCAGCACA
    CCTTCAATGA GCACCATCAC GCACTCAGCT CACATGGACA TCTTCCAGAA CCTGGCTGTG
    GATTTGGACA CTGAAGGTCG GTATCTCTTC TTGAATGCAA TTGCAAATCA GCTGCGGTAC
    CCAAATAGCC ACACTCACTA CTTCAGCTGC ACCATGCTGT ACCTTTTTGC AGAGGCCAAC
    ACTGAAGCTA TTCAAGAACA GATCACAAGG GTTCTTTTGG AACGGTTGAT TGTAAATAGG
    CCACATCCTT GGGGTCTTCT TATTACCTTC ATTGAGCTGA TTAAAAATCC AGCATTTAAA
    TTCTGGAACC ATGAATTTGT ACACTGTGCC CCAGAAATTG AAAAGTTATT CCAGTCCGTC
    GCACAGTGCT GCATGGGACA AAAGCAGGCT CAGCAAGTAA TGGAAGGGAC AGGTGCCAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225095
    Clone ID Related Accession (Same CDS sequence) XM_024978375.1
    Accession Version XM_024978375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGAGTAAGAT GAAACCTTCG
    GACTTGTCTC AGGTGTGGCC AGAGGCAAAT CAGCACTTTA GTAAAGAGAT AGATGATGAA
    GCAAACAGCT ATTTCCAGAG AATATATAAT CACCCACCAC ATCCAACCAT GTCTGTTGAT
    GAGGTATTAG AAATGCTACA GAGATTTAAA GATTCTACCA TAAAGAGGGA ACGAGAAGTA
    TTTAACTGTA TGCTAAGGAA CTTGTTCGAA GAATATCGGT TCTTCCCCCA ATACCCTGAT
    AAAGAGTTAC ATATAACAGC CTGCCTGTTT GGTGGAATAA TCGAAAAAGG ACTGGTCACT
    TATATGGCAC TAGGTTTGGC CCTGCGATAC GTTCTTGAAG CCTTACGCAA GCCTTTTGGA
    TCCAAAATGT ATTATTTTGG GATTGCTGCA CTAGATAGAT TTAAAAATAG ATTGAAGGAC
    TATCCCCAGT ATTGTCAGCA CTTGGCTTCG ATCAGTCACT TTATGCAATT TCCACATCAT
    TTACAGGAGT ATATTGAGTA TGGACAGCAA TCTAGAGATC CTCCTGTGAA AATGCAGGGC
    TCTATCACAA CCCCTGGAAG TATTGCACTG GCTCAAGCCC AGGCCCAGGC CCAGGTTCCA
    GCAAAAGCTC CCCTTGCTGG CCAGGTTAGC ACTATGGTAA CCACCTCAAC AACCACCACT
    GTTGCTAAAA CGGTTACAGT CACCAGGCCA ACTGGAGTCA GCTTTAAGAA AGATGTGCCA
    CCTTCTATTA ATACAACAAA TATAGATACA TTGCTTGTGG CCACAGATCA AACTGAGAGA
    ATCGTAGAGC CCCCAGAAAA TATCCAGGAG AAAATTGCTT TTATCTTCAA CAACCTTTCC
    CAGTCAAATA TGACACAAAA GGTTGAAGAA CTAAAGGAAA CTGTGAAGGA AGAATTTATG
    CCTTGGGTTT CACAGTACCT GGTTATGAAG AGAGTCAGTA TTGAGCCAAA CTTTCACAGC
    CTGTATTCTA ACTTCCTTGA CACGCTGAAG AATCCTGAGT TTAATAAGAT GGTTCTGAAT
    GAGACCTATA GAAACATCAA AGTACTCCTG ACCTCTGATA AAGCTGCAGC CAATTTCTCT
    GATCGGTCTT TGCTGAAGAA CTTGGGACAT TGGCTAGGAA TGATTACACT AGCTAAAAAT
    AAACCCATCT TACACACTGA CTTGGATGTA AAATCTTTGC TGCTAGAGGC TTATGTTAAA
    GGACAGCAAG AGTTGCTCTA TGTAGTGCCC TTTGTTGCCA AAGTCTTAGA ATCTAGCATT
    CGTAGTGTGG TTTTTAGGCC ACCCAACCCC TGGACAATGG CTATTATGAA TGTATTAGCT
    GAGCTACATC AAGAACATGA CTTAAAGTTA AACTTGAAGT TTGAAATTGA GGTCCTCTGT
    AAGAATCTTG CATTAGACAT CAATGAGCTA AAACCTGGAA ACCTCCTAAA GGATAAAGAT
    CGCCTGAAGA ACTTAGATGA GCAACTCTCT GCTCCAAAGA AAGATGTCAA GCAGCCAGAG
    GAGCTCCCTC CTATCACAAC CACAACAACT TCTACAACAC CAGCTACCAG CACCACCTGT
    ACAGCCACTG TTCCACCACA GCCACAGTAC AGTTACCATG ACATCAATGT CTATTCCCTC
    GCAGGCCTGG CACCACACAT TACTCTAAAC CCAACAATTC CCTTGTTTCA GGCCCATCCA
    CAGCTGAAGC AGTGTGTGCG CCAAGCAATC GAACGGGCTG TCCAGGAGCT GGTCCATCCT
    GTGGTGGATC GATCAATTAA GATTGCCATG ACTACTTGTG AGCAAATAGT CAGGAAAGAT
    TTTGCCCTGG ATTCTGAGGA ATCTCGAATG CGAATAGCAG CTCATCACAT GATGCGGAAC
    TTGACAGCTG GAATGGCTAT GATTACTTGC AGGGAACCTT TACTCATGAG CATATCAACC
    AACCTGAAAA ACAGTTTTGC CTCAGCCCTT CGTACTGCAA GCCCACAACA AAGGGAAATG
    ATGGATCAGG CAGCTGCTCA ATTAGCTCAG GACAATTGTG AATTAGCTTG CTGTTTTATT
    CAGAAGACTG CAGTAGAAAA AGCAGGCCCT GAGATGGACA AGAGATTAGC AACTGAATTT
    GAGCTGAGAA AACATGCTAG GCAAGAAGGG CGAAGGTATT GTGATCCTGT TGTGTTAACA
    TACCAAGCTG AGCGGATGCC AGAGCAAATC AGGCTGAAAG TTGGTGGTGT GGACCCAAAG
    CAGCTGGCTG TTTATGAAGA GTTTGCACGC AATGTGCCTG GCTTCTTGCC TACAAATGAT
    TTAAGTCAGC CCACAGGATT TTTAGCCCAG CCCATGAAGC AAGCTTGGGC AACAGATGAT
    GTAGCTCAGA TTTATGATAA GTGTATTACA GAACTGGAGC AGCATCTTCA CGCTATCCCG
    CCAACTTTGG CCATGAACCC CCAAGCTCAG GCCCTCAGAA GTCTTCTGGA GGTTGTAGTA
    TTATCACGGA ACTCTCGAGA TGCCATTGCT GCTCTTGGAT TGCTCCAGAA GGCTGTAGAG
    GGCTTACTGG ATGCCACCAG TGGTGCTGAT GCTGACCTCC TTCTTCGCTA CAGAGAGTGC
    CACCTCTTGG TCTTGAAAGC TCTTCAAGAT GGCCGGGCAT ATGGGTCTCC ATGGTGTAAC
    AAACAGATCA CAAGGTGCCT AATTGAGTGT CGGGATGAAT ACAAATATAA TGTGGAGGCA
    GTGGAGTTGC TAATTCGCAA TCATTTGGTC AACATGCAAC AGTATGATCT TCACCTTGCT
    CAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834143.1
    CDS328..7095
    Translation

    Target ORF information:

    RefSeq Version XM_024978375.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGTTTAT TTGAAAAAAC ACAGGGTAAA GTTTATTGTG
    AAGGCTTTGT CAATGCTGCT AAATGGTACT CCATTTGCCT TTGTTATTGA CCTTGCTGCA
    CTTGCTTCAC GTCGTGAATA CCTCAAACTT GACAAATGGC TCACAGATAA AATTCGAGAG
    CATGGAGAGC CTTTTATCCA GGCTTGTATG ACTTTTTTAA AGAGACGGTG TCCTTCTATT
    TTGGGGGGAC TTGCCCCAGA GAAAGATCAG CCTAAAAGTG CTCAACTTCC TCCAGAAACC
    CTCGCGACAA TGTTGGCCTG TTTACAAGCT TGTGCAGGGA GTGTTTCTCA GGAGTTATCA
    GAAACCATCC TCACCATGGT GGCCAATTGC AGTAACGTTA TGAACAAGGC CAGGCAGCCA
    CCACCGGGAG TTATGCCAAA AGGACGCCCT CCTAGTGCTA GCAGCTTGGA CGCCATTTCT
    CCCGTTCAGA TTGACCCTCT TGCTGGAATG GCATCTCTTA GTATAGGTGG TTCAGCTGCC
    CCTCACACCC AGAGTATGCA GGGCTTTCCT CCAAATTTGG GTTCTGCATT CAGTACCCCA
    CAGTCACCAG CAAAAGCATT TCCACCCCTT TCAACCCCAA ATCAGACAAC TGCATTCAGT
    GGTATTGGAG GACTTTCATC ACAGCTTCCA GTAGGTGGTC TTGGCACAGG CAGCCTGACT
    GGTATAGGAA CTGGGGCTCT TGGACTCCCT GCAGTGAATA GCGATCCTTT TGTACAGAGG
    AAACTGGGCA CCTCTGGACT GAACCAGCCT ACATTCCAGC AGAGTAAGAT GAAACCTTCG
    GACTTGTCTC AGGTGTGGCC AGAGGCAAAT CAGCACTTTA GTAAAGAGAT AGATGATGAA
    GCAAACAGCT ATTTCCAGAG AATATATAAT CACCCACCAC ATCCAACCAT GTCTGTTGAT
    GAGGTATTAG AAATGCTACA GAGATTTAAA GATTCTACCA TAAAGAGGGA ACGAGAAGTA
    TTTAACTGTA TGCTAAGGAA CTTGTTCGAA GAATATCGGT TCTTCCCCCA ATACCCTGAT
    AAAGAGTTAC ATATAACAGC CTGCCTGTTT GGTGGAATAA TCGAAAAAGG ACTGGTCACT
    TATATGGCAC TAGGTTTGGC CCTGCGATAC GTTCTTGAAG CCTTACGCAA GCCTTTTGGA
    TCCAAAATGT ATTATTTTGG GATTGCTGCA CTAGATAGAT TTAAAAATAG ATTGAAGGAC
    TATCCCCAGT ATTGTCAGCA CTTGGCTTCG ATCAGTCACT TTATGCAATT TCCACATCAT
    TTACAGGAGT ATATTGAGTA TGGACAGCAA TCTAGAGATC CTCCTGTGAA AATGCAGGGC
    TCTATCACAA CCCCTGGAAG TATTGCACTG GCTCAAGCCC AGGCCCAGGC CCAGGTTCCA
    GCAAAAGCTC CCCTTGCTGG CCAGGTTAGC ACTATGGTAA CCACCTCAAC AACCACCACT
    GTTGCTAAAA CGGTTACAGT CACCAGGCCA ACTGGAGTCA GCTTTAAGAA AGATGTGCCA
    CCTTCTATTA ATACAACAAA TATAGATACA TTGCTTGTGG CCACAGATCA AACTGAGAGA
    ATCGTAGAGC CCCCAGAAAA TATCCAGGAG AAAATTGCTT TTATCTTCAA CAACCTTTCC
    CAGTCAAATA TGACACAAAA GGTTGAAGAA CTAAAGGAAA CTGTGAAGGA AGAATTTATG
    CCTTGGGTTT CACAGTACCT GGTTATGAAG AGAGTCAGTA TTGAGCCAAA CTTTCACAGC
    CTGTATTCTA ACTTCCTTGA CACGCTGAAG AATCCTGAGT TTAATAAGAT GGTTCTGAAT
    GAGACCTATA GAAACATCAA AGTACTCCTG ACCTCTGATA AAGCTGCAGC CAATTTCTCT
    GATCGGTCTT TGCTGAAGAA CTTGGGACAT TGGCTAGGAA TGATTACACT AGCTAAAAAT
    AAACCCATCT TACACACTGA CTTGGATGTA AAATCTTTGC TGCTAGAGGC TTATGTTAAA
    GGACAGCAAG AGTTGCTCTA TGTAGTGCCC TTTGTTGCCA AAGTCTTAGA ATCTAGCATT
    CGTAGTGTGG TTTTTAGGCC ACCCAACCCC TGGACAATGG CTATTATGAA TGTATTAGCT
    GAGCTACATC AAGAACATGA CTTAAAGTTA AACTTGAAGT TTGAAATTGA GGTCCTCTGT
    AAGAATCTTG CATTAGACAT CAATGAGCTA AAACCTGGAA ACCTCCTAAA GGATAAAGAT
    CGCCTGAAGA ACTTAGATGA GCAACTCTCT GCTCCAAAGA AAGATGTCAA GCAGCCAGAG
    GAGCTCCCTC CTATCACAAC CACAACAACT TCTACAACAC CAGCTACCAG CACCACCTGT
    ACAGCCACTG TTCCACCACA GCCACAGTAC AGTTACCATG ACATCAATGT CTATTCCCTC
    GCAGGCCTGG CACCACACAT TACTCTAAAC CCAACAATTC CCTTGTTTCA GGCCCATCCA
    CAGCTGAAGC AGTGTGTGCG CCAAGCAATC GAACGGGCTG TCCAGGAGCT GGTCCATCCT
    GTGGTGGATC GATCAATTAA GATTGCCATG ACTACTTGTG AGCAAATAGT CAGGAAAGAT
    TTTGCCCTGG ATTCTGAGGA ATCTCGAATG CGAATAGCAG CTCATCACAT GATGCGGAAC
    TTGACAGCTG GAATGGCTAT GATTACTTGC AGGGAACCTT TACTCATGAG CATATCAACC
    AACCTGAAAA ACAGTTTTGC CTCAGCCCTT CGTACTGCAA GCCCACAACA AAGGGAAATG
    ATGGATCAGG CAGCTGCTCA ATTAGCTCAG GACAATTGTG AATTAGCTTG CTGTTTTATT
    CAGAAGACTG CAGTAGAAAA AGCAGGCCCT GAGATGGACA AGAGATTAGC AACTGAATTT
    GAGCTGAGAA AACATGCTAG GCAAGAAGGG CGAAGGTATT GTGATCCTGT TGTGTTAACA
    TACCAAGCTG AGCGGATGCC AGAGCAAATC AGGCTGAAAG TTGGTGGTGT GGACCCAAAG
    CAGCTGGCTG TTTATGAAGA GTTTGCACGC AATGTGCCTG GCTTCTTGCC TACAAATGAT
    TTAAGTCAGC CCACAGGATT TTTAGCCCAG CCCATGAAGC AAGCTTGGGC AACAGATGAT
    GTAGCTCAGA TTTATGATAA GTGTATTACA GAACTGGAGC AGCATCTTCA CGCTATCCCG
    CCAACTTTGG CCATGAACCC CCAAGCTCAG GCCCTCAGAA GTCTTCTGGA GGTTGTAGTA
    TTATCACGGA ACTCTCGAGA TGCCATTGCT GCTCTTGGAT TGCTCCAGAA GGCTGTAGAG
    GGCTTACTGG ATGCCACCAG TGGTGCTGAT GCTGACCTCC TTCTTCGCTA CAGAGAGTGC
    CACCTCTTGG TCTTGAAAGC TCTTCAAGAT GGCCGGGCAT ATGGGTCTCC ATGGTGTAAC
    AAACAGATCA CAAGGTGCCT AATTGAGTGT CGGGATGAAT ACAAATATAA TGTGGAGGCA
    GTGGAGTTGC TAATTCGCAA TCATTTGGTC AACATGCAAC AGTATGATCT TCACCTTGCT
    CAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40207
    Clone ID Related Accession (Same CDS sequence) NM_001206039.1
    Accession Version NM_001206039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000018.1. On Apr 20, 2011 this sequence version replaced XM_613555.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.48866.1, SRR5765710.213.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATTGCC CCAACTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG
    ATTGATCGTG CTCATGGAGG CCCTAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA
    GAGTATGATG ATCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG
    AATCTCTACC ATTCAGCAGC AGCTGGTCGT GACAGTACCA AAGCTTTCTC TGCGTTTGTT
    GGACAGATGC ACCAGCAAGG AATCCTGAAG ACTGATGACC TCATAACAAG GTTCTTTCGT
    CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCAGAGCA GCAGCACAAT
    CCTGCTGCTA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTC
    CGACTCATTG CACTGCTAGT AAAGCACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT
    AATCTGCTGA ATAAGGTCCT CGGTATAGTA GTGGGAGTTC TCCTACAGGA TCATGATGTT
    CGGCAAAGTG AATTTCAGCA ACTTCCCTAC CACCGAATTT TTATCATGCT GCTCTTGGAA
    CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC
    AACACATTCC ACATTTTGAG GCCTACCAAA GCTCCTGGCT TTGTGTATGC TTGGCTTGAA
    CTGATCTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ACACGCCACA GCAGAAGGGA
    TGGCCTATGT ATGCACAGTT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA
    AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG GGTGCTGCTG
    GTTCTTTTGC ATGATTTTCC TGAGTTCCTT TGTGATTACC ATTATGGATT TTGTGACGTG
    ATCCCACCTA ATTGTATCCA GCTAAGAAAT CTGATCCTGA GTGCCTTCCC AAGAAACATG
    AGGCTCCCAG ACCCATTCAC TCCTAATCTG AAGGTGGATA TGTTGAGTGA AATTAACATC
    GCTCCCCGGA TTCTCACCAA TTTTACTGGA GTGATGCCAC CTCAATTCAA AAAGGATTTG
    GATTCCTATC TTAAAACTCG ATCACCTGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA
    CAGGTATCCA ATGAGCCTGG TAATCGCTAC AACCTCCAGC TCATTAATGC ACTGGTGCTC
    TATGTAGGGA CTCAGGCCAT TGCACACATC CACAACAAGG GCAGCACACC TTCAATGAGC
    ACCATCACGC ACTCAGCTCA CATGGACATC TTCCAGAACC TGGCTGTGGA TTTGGACACT
    GAAGGTCGGT ATCTCTTCTT GAATGCAATT GCAAATCAGC TGCGGTACCC AAATAGCCAC
    ACTCACTACT TCAGCTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAACAC TGAAGCTATT
    CAAGAACAGA TCACAAGGGT TCTTTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG
    GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAATCCAG CATTTAAATT CTGGAACCAT
    GAATTTGTAC ACTGTGCCCC AGAAATTGAA AAGTTATTCC AGTCCGTCGC ACAGTGCTGC
    ATGGGACAAA AGCAGGCTCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192968.1
    CDS292..7422
    Translation

    Target ORF information:

    RefSeq Version NM_001206039.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATTGCC CCAACTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG
    ATTGATCGTG CTCATGGAGG CCCTAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA
    GAGTATGATG ATCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG
    AATCTCTACC ATTCAGCAGC AGCTGGTCGT GACAGTACCA AAGCTTTCTC TGCGTTTGTT
    GGACAGATGC ACCAGCAAGG AATCCTGAAG ACTGATGACC TCATAACAAG GTTCTTTCGT
    CTGTGTACTG AAATGTGTGT TGAAATCAGT TACCGTGCTC AGGCAGAGCA GCAGCACAAT
    CCTGCTGCTA ATCCCACCAT GATCCGAGCC AAGTGCTATC ACAACCTGGA TGCCTTTGTC
    CGACTCATTG CACTGCTAGT AAAGCACTCA GGGGAGGCCA CCAACACTGT CACAAAGATT
    AATCTGCTGA ATAAGGTCCT CGGTATAGTA GTGGGAGTTC TCCTACAGGA TCATGATGTT
    CGGCAAAGTG AATTTCAGCA ACTTCCCTAC CACCGAATTT TTATCATGCT GCTCTTGGAA
    CTCAATGCAC CTGAGCATGT GTTGGAAACC ATTAATTTCC AGACACTTAC AGCTTTCTGC
    AACACATTCC ACATTTTGAG GCCTACCAAA GCTCCTGGCT TTGTGTATGC TTGGCTTGAA
    CTGATCTCCC ATCGGATATT TATTGCAAGA ATGCTGGCAC ACACGCCACA GCAGAAGGGA
    TGGCCTATGT ATGCACAGTT ACTGATTGAT TTATTCAAAT ATTTAGCGCC TTTCCTTAGA
    AATGTGGAAC TCACCAAACC TATGCAAATC CTCTACAAGG GCACTTTAAG GGTGCTGCTG
    GTTCTTTTGC ATGATTTTCC TGAGTTCCTT TGTGATTACC ATTATGGATT TTGTGACGTG
    ATCCCACCTA ATTGTATCCA GCTAAGAAAT CTGATCCTGA GTGCCTTCCC AAGAAACATG
    AGGCTCCCAG ACCCATTCAC TCCTAATCTG AAGGTGGATA TGTTGAGTGA AATTAACATC
    GCTCCCCGGA TTCTCACCAA TTTTACTGGA GTGATGCCAC CTCAATTCAA AAAGGATTTG
    GATTCCTATC TTAAAACTCG ATCACCTGTC ACTTTCCTGT CTGATCTGCG CAGCAACCTA
    CAGGTATCCA ATGAGCCTGG TAATCGCTAC AACCTCCAGC TCATTAATGC ACTGGTGCTC
    TATGTAGGGA CTCAGGCCAT TGCACACATC CACAACAAGG GCAGCACACC TTCAATGAGC
    ACCATCACGC ACTCAGCTCA CATGGACATC TTCCAGAACC TGGCTGTGGA TTTGGACACT
    GAAGGTCGGT ATCTCTTCTT GAATGCAATT GCAAATCAGC TGCGGTACCC AAATAGCCAC
    ACTCACTACT TCAGCTGCAC CATGCTGTAC CTTTTTGCAG AGGCCAACAC TGAAGCTATT
    CAAGAACAGA TCACAAGGGT TCTTTTGGAA CGGTTGATTG TAAATAGGCC ACATCCTTGG
    GGTCTTCTTA TTACCTTCAT TGAGCTGATT AAAAATCCAG CATTTAAATT CTGGAACCAT
    GAATTTGTAC ACTGTGCCCC AGAAATTGAA AAGTTATTCC AGTCCGTCGC ACAGTGCTGC
    ATGGGACAAA AGCAGGCTCA GCAAGTAATG GAAGGGACAG GTGCCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28465
    Clone ID Related Accession (Same CDS sequence) XM_005218664.4 , XM_005218664.3
    Accession Version XM_005218664.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218664.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GGTAGAGCTT
    TTGGAAAGAA AGATGCACCA GCAAGGAATC CTGAAGACTG ATGACCTCAT AACAAGGTTC
    TTTCGTCTGT GTACTGAAAT GTGTGTTGAA ATCAGTTACC GTGCTCAGGC AGAGCAGCAG
    CACAATCCTG CTGCTAATCC CACCATGATC CGAGCCAAGT GCTATCACAA CCTGGATGCC
    TTTGTCCGAC TCATTGCACT GCTAGTAAAG CACTCAGGGG AGGCCACCAA CACTGTCACA
    AAGATTAATC TGCTGAATAA GGTCCTCGGT ATAGTAGTGG GAGTTCTCCT ACAGGATCAT
    GATGTTCGGC AAAGTGAATT TCAGCAACTT CCCTACCACC GAATTTTTAT CATGCTGCTC
    TTGGAACTCA ATGCACCTGA GCATGTGTTG GAAACCATTA ATTTCCAGAC ACTTACAGCT
    TTCTGCAACA CATTCCACAT TTTGAGGCCT ACCAAAGCTC CTGGCTTTGT GTATGCTTGG
    CTTGAACTGA TCTCCCATCG GATATTTATT GCAAGAATGC TGGCACACAC GCCACAGCAG
    AAGGGATGGC CTATGTATGC ACAGTTACTG ATTGATTTAT TCAAATATTT AGCGCCTTTC
    CTTAGAAATG TGGAACTCAC CAAACCTATG CAAATCCTCT ACAAGGGCAC TTTAAGGGTG
    CTGCTGGTTC TTTTGCATGA TTTTCCTGAG TTCCTTTGTG ATTACCATTA TGGATTTTGT
    GACGTGATCC CACCTAATTG TATCCAGCTA AGAAATCTGA TCCTGAGTGC CTTCCCAAGA
    AACATGAGGC TCCCAGACCC ATTCACTCCT AATCTGAAGG TGGATATGTT GAGTGAAATT
    AACATCGCTC CCCGGATTCT CACCAATTTT ACTGGAGTGA TGCCACCTCA ATTCAAAAAG
    GATTTGGATT CCTATCTTAA AACTCGATCA CCTGTCACTT TCCTGTCTGA TCTGCGCAGC
    AACCTACAGG TATCCAATGA GCCTGGTAAT CGCTACAACC TCCAGCTCAT TAATGCACTG
    GTGCTCTATG TAGGGACTCA GGCCATTGCA CACATCCACA ACAAGGGCAG CACACCTTCA
    ATGAGCACCA TCACGCACTC AGCTCACATG GACATCTTCC AGAACCTGGC TGTGGATTTG
    GACACTGAAG GTCGGTATCT CTTCTTGAAT GCAATTGCAA ATCAGCTGCG GTACCCAAAT
    AGCCACACTC ACTACTTCAG CTGCACCATG CTGTACCTTT TTGCAGAGGC CAACACTGAA
    GCTATTCAAG AACAGATCAC AAGGGTTCTT TTGGAACGGT TGATTGTAAA TAGGCCACAT
    CCTTGGGGTC TTCTTATTAC CTTCATTGAG CTGATTAAAA ATCCAGCATT TAAATTCTGG
    AACCATGAAT TTGTACACTG TGCCCCAGAA ATTGAAAAGT TATTCCAGTC CGTCGCACAG
    TGCTGCATGG GACAAAAGCA GGCTCAGCAA GTAATGGAAG GGACAGGTGC CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218721.1
    CDS334..7470
    Translation

    Target ORF information:

    RefSeq Version XM_005218664.4
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218664.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GGTAGAGCTT
    TTGGAAAGAA AGATGCACCA GCAAGGAATC CTGAAGACTG ATGACCTCAT AACAAGGTTC
    TTTCGTCTGT GTACTGAAAT GTGTGTTGAA ATCAGTTACC GTGCTCAGGC AGAGCAGCAG
    CACAATCCTG CTGCTAATCC CACCATGATC CGAGCCAAGT GCTATCACAA CCTGGATGCC
    TTTGTCCGAC TCATTGCACT GCTAGTAAAG CACTCAGGGG AGGCCACCAA CACTGTCACA
    AAGATTAATC TGCTGAATAA GGTCCTCGGT ATAGTAGTGG GAGTTCTCCT ACAGGATCAT
    GATGTTCGGC AAAGTGAATT TCAGCAACTT CCCTACCACC GAATTTTTAT CATGCTGCTC
    TTGGAACTCA ATGCACCTGA GCATGTGTTG GAAACCATTA ATTTCCAGAC ACTTACAGCT
    TTCTGCAACA CATTCCACAT TTTGAGGCCT ACCAAAGCTC CTGGCTTTGT GTATGCTTGG
    CTTGAACTGA TCTCCCATCG GATATTTATT GCAAGAATGC TGGCACACAC GCCACAGCAG
    AAGGGATGGC CTATGTATGC ACAGTTACTG ATTGATTTAT TCAAATATTT AGCGCCTTTC
    CTTAGAAATG TGGAACTCAC CAAACCTATG CAAATCCTCT ACAAGGGCAC TTTAAGGGTG
    CTGCTGGTTC TTTTGCATGA TTTTCCTGAG TTCCTTTGTG ATTACCATTA TGGATTTTGT
    GACGTGATCC CACCTAATTG TATCCAGCTA AGAAATCTGA TCCTGAGTGC CTTCCCAAGA
    AACATGAGGC TCCCAGACCC ATTCACTCCT AATCTGAAGG TGGATATGTT GAGTGAAATT
    AACATCGCTC CCCGGATTCT CACCAATTTT ACTGGAGTGA TGCCACCTCA ATTCAAAAAG
    GATTTGGATT CCTATCTTAA AACTCGATCA CCTGTCACTT TCCTGTCTGA TCTGCGCAGC
    AACCTACAGG TATCCAATGA GCCTGGTAAT CGCTACAACC TCCAGCTCAT TAATGCACTG
    GTGCTCTATG TAGGGACTCA GGCCATTGCA CACATCCACA ACAAGGGCAG CACACCTTCA
    ATGAGCACCA TCACGCACTC AGCTCACATG GACATCTTCC AGAACCTGGC TGTGGATTTG
    GACACTGAAG GTCGGTATCT CTTCTTGAAT GCAATTGCAA ATCAGCTGCG GTACCCAAAT
    AGCCACACTC ACTACTTCAG CTGCACCATG CTGTACCTTT TTGCAGAGGC CAACACTGAA
    GCTATTCAAG AACAGATCAC AAGGGTTCTT TTGGAACGGT TGATTGTAAA TAGGCCACAT
    CCTTGGGGTC TTCTTATTAC CTTCATTGAG CTGATTAAAA ATCCAGCATT TAAATTCTGG
    AACCATGAAT TTGTACACTG TGCCCCAGAA ATTGAAAAGT TATTCCAGTC CGTCGCACAG
    TGCTGCATGG GACAAAAGCA GGCTCAGCAA GTAATGGAAG GGACAGGTGC CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28465
    Clone ID Related Accession (Same CDS sequence) XM_005218664.4 , XM_005218664.3
    Accession Version XM_005218664.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GGTAGAGCTT
    TTGGAAAGAA AGATGCACCA GCAAGGAATC CTGAAGACTG ATGACCTCAT AACAAGGTTC
    TTTCGTCTGT GTACTGAAAT GTGTGTTGAA ATCAGTTACC GTGCTCAGGC AGAGCAGCAG
    CACAATCCTG CTGCTAATCC CACCATGATC CGAGCCAAGT GCTATCACAA CCTGGATGCC
    TTTGTCCGAC TCATTGCACT GCTAGTAAAG CACTCAGGGG AGGCCACCAA CACTGTCACA
    AAGATTAATC TGCTGAATAA GGTCCTCGGT ATAGTAGTGG GAGTTCTCCT ACAGGATCAT
    GATGTTCGGC AAAGTGAATT TCAGCAACTT CCCTACCACC GAATTTTTAT CATGCTGCTC
    TTGGAACTCA ATGCACCTGA GCATGTGTTG GAAACCATTA ATTTCCAGAC ACTTACAGCT
    TTCTGCAACA CATTCCACAT TTTGAGGCCT ACCAAAGCTC CTGGCTTTGT GTATGCTTGG
    CTTGAACTGA TCTCCCATCG GATATTTATT GCAAGAATGC TGGCACACAC GCCACAGCAG
    AAGGGATGGC CTATGTATGC ACAGTTACTG ATTGATTTAT TCAAATATTT AGCGCCTTTC
    CTTAGAAATG TGGAACTCAC CAAACCTATG CAAATCCTCT ACAAGGGCAC TTTAAGGGTG
    CTGCTGGTTC TTTTGCATGA TTTTCCTGAG TTCCTTTGTG ATTACCATTA TGGATTTTGT
    GACGTGATCC CACCTAATTG TATCCAGCTA AGAAATCTGA TCCTGAGTGC CTTCCCAAGA
    AACATGAGGC TCCCAGACCC ATTCACTCCT AATCTGAAGG TGGATATGTT GAGTGAAATT
    AACATCGCTC CCCGGATTCT CACCAATTTT ACTGGAGTGA TGCCACCTCA ATTCAAAAAG
    GATTTGGATT CCTATCTTAA AACTCGATCA CCTGTCACTT TCCTGTCTGA TCTGCGCAGC
    AACCTACAGG TATCCAATGA GCCTGGTAAT CGCTACAACC TCCAGCTCAT TAATGCACTG
    GTGCTCTATG TAGGGACTCA GGCCATTGCA CACATCCACA ACAAGGGCAG CACACCTTCA
    ATGAGCACCA TCACGCACTC AGCTCACATG GACATCTTCC AGAACCTGGC TGTGGATTTG
    GACACTGAAG GTCGGTATCT CTTCTTGAAT GCAATTGCAA ATCAGCTGCG GTACCCAAAT
    AGCCACACTC ACTACTTCAG CTGCACCATG CTGTACCTTT TTGCAGAGGC CAACACTGAA
    GCTATTCAAG AACAGATCAC AAGGGTTCTT TTGGAACGGT TGATTGTAAA TAGGCCACAT
    CCTTGGGGTC TTCTTATTAC CTTCATTGAG CTGATTAAAA ATCCAGCATT TAAATTCTGG
    AACCATGAAT TTGTACACTG TGCCCCAGAA ATTGAAAAGT TATTCCAGTC CGTCGCACAG
    TGCTGCATGG GACAAAAGCA GGCTCAGCAA GTAATGGAAG GGACAGGTGC CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218721.1
    CDS311..7447
    Translation

    Target ORF information:

    RefSeq Version XM_005218664.3
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218664.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GGTAGAGCTT
    TTGGAAAGAA AGATGCACCA GCAAGGAATC CTGAAGACTG ATGACCTCAT AACAAGGTTC
    TTTCGTCTGT GTACTGAAAT GTGTGTTGAA ATCAGTTACC GTGCTCAGGC AGAGCAGCAG
    CACAATCCTG CTGCTAATCC CACCATGATC CGAGCCAAGT GCTATCACAA CCTGGATGCC
    TTTGTCCGAC TCATTGCACT GCTAGTAAAG CACTCAGGGG AGGCCACCAA CACTGTCACA
    AAGATTAATC TGCTGAATAA GGTCCTCGGT ATAGTAGTGG GAGTTCTCCT ACAGGATCAT
    GATGTTCGGC AAAGTGAATT TCAGCAACTT CCCTACCACC GAATTTTTAT CATGCTGCTC
    TTGGAACTCA ATGCACCTGA GCATGTGTTG GAAACCATTA ATTTCCAGAC ACTTACAGCT
    TTCTGCAACA CATTCCACAT TTTGAGGCCT ACCAAAGCTC CTGGCTTTGT GTATGCTTGG
    CTTGAACTGA TCTCCCATCG GATATTTATT GCAAGAATGC TGGCACACAC GCCACAGCAG
    AAGGGATGGC CTATGTATGC ACAGTTACTG ATTGATTTAT TCAAATATTT AGCGCCTTTC
    CTTAGAAATG TGGAACTCAC CAAACCTATG CAAATCCTCT ACAAGGGCAC TTTAAGGGTG
    CTGCTGGTTC TTTTGCATGA TTTTCCTGAG TTCCTTTGTG ATTACCATTA TGGATTTTGT
    GACGTGATCC CACCTAATTG TATCCAGCTA AGAAATCTGA TCCTGAGTGC CTTCCCAAGA
    AACATGAGGC TCCCAGACCC ATTCACTCCT AATCTGAAGG TGGATATGTT GAGTGAAATT
    AACATCGCTC CCCGGATTCT CACCAATTTT ACTGGAGTGA TGCCACCTCA ATTCAAAAAG
    GATTTGGATT CCTATCTTAA AACTCGATCA CCTGTCACTT TCCTGTCTGA TCTGCGCAGC
    AACCTACAGG TATCCAATGA GCCTGGTAAT CGCTACAACC TCCAGCTCAT TAATGCACTG
    GTGCTCTATG TAGGGACTCA GGCCATTGCA CACATCCACA ACAAGGGCAG CACACCTTCA
    ATGAGCACCA TCACGCACTC AGCTCACATG GACATCTTCC AGAACCTGGC TGTGGATTTG
    GACACTGAAG GTCGGTATCT CTTCTTGAAT GCAATTGCAA ATCAGCTGCG GTACCCAAAT
    AGCCACACTC ACTACTTCAG CTGCACCATG CTGTACCTTT TTGCAGAGGC CAACACTGAA
    GCTATTCAAG AACAGATCAC AAGGGTTCTT TTGGAACGGT TGATTGTAAA TAGGCCACAT
    CCTTGGGGTC TTCTTATTAC CTTCATTGAG CTGATTAAAA ATCCAGCATT TAAATTCTGG
    AACCATGAAT TTGTACACTG TGCCCCAGAA ATTGAAAAGT TATTCCAGTC CGTCGCACAG
    TGCTGCATGG GACAAAAGCA GGCTCAGCAA GTAATGGAAG GGACAGGTGC CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28467
    Clone ID Related Accession (Same CDS sequence) XM_005218666.3 , XM_005218666.4
    Accession Version XM_005218666.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGT
    AAAGAGATAG ATGATGAAGC AAACAGCTAT TTCCAGAGAA TATATAATCA CCCACCACAT
    CCAACCATGT CTGTTGATGA GGTATTAGAA ATGCTACAGA GATTTAAAGA TTCTACCATA
    AAGAGGGAAC GAGAAGTATT TAACTGTATG CTAAGGAACT TGTTCGAAGA ATATCGGTTC
    TTCCCCCAAT ACCCTGATAA AGAGTTACAT ATAACAGCCT GCCTGTTTGG TGGAATAATC
    GAAAAAGGAC TGGTCACTTA TATGGCACTA GGTTTGGCCC TGCGATACGT TCTTGAAGCC
    TTACGCAAGC CTTTTGGATC CAAAATGTAT TATTTTGGGA TTGCTGCACT AGATAGATTT
    AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCGAT CAGTCACTTT
    ATGCAATTTC CACATCATTT ACAGGAGTAT ATTGAGTATG GACAGCAATC TAGAGATCCT
    CCTGTGAAAA TGCAGGGCTC TATCACAACC CCTGGAAGTA TTGCACTGGC TCAAGCCCAG
    GCCCAGGCCC AGGTTCCAGC AAAAGCTCCC CTTGCTGGCC AGGTTAGCAC TATGGTAACC
    ACCTCAACAA CCACCACTGT TGCTAAAACG GTTACAGTCA CCAGGCCAAC TGGAGTCAGC
    TTTAAGAAAG ATGTGCCACC TTCTATTAAT ACAACAAATA TAGATACATT GCTTGTGGCC
    ACAGATCAAA CTGAGAGAAT CGTAGAGCCC CCAGAAAATA TCCAGGAGAA AATTGCTTTT
    ATCTTCAACA ACCTTTCCCA GTCAAATATG ACACAAAAGG TTGAAGAACT AAAGGAAACT
    GTGAAGGAAG AATTTATGCC TTGGGTTTCA CAGTACCTGG TTATGAAGAG AGTCAGTATT
    GAGCCAAACT TTCACAGCCT GTATTCTAAC TTCCTTGACA CGCTGAAGAA TCCTGAGTTT
    AATAAGATGG TTCTGAATGA GACCTATAGA AACATCAAAG TACTCCTGAC CTCTGATAAA
    GCTGCAGCCA ATTTCTCTGA TCGGTCTTTG CTGAAGAACT TGGGACATTG GCTAGGAATG
    ATTACACTAG CTAAAAATAA ACCCATCTTA CACACTGACT TGGATGTAAA ATCTTTGCTG
    CTAGAGGCTT ATGTTAAAGG ACAGCAAGAG TTGCTCTATG TAGTGCCCTT TGTTGCCAAA
    GTCTTAGAAT CTAGCATTCG TAGTGTGGTT TTTAGGCCAC CCAACCCCTG GACAATGGCT
    ATTATGAATG TATTAGCTGA GCTACATCAA GAACATGACT TAAAGTTAAA CTTGAAGTTT
    GAAATTGAGG TCCTCTGTAA GAATCTTGCA TTAGACATCA ATGAGCTAAA ACCTGGAAAC
    CTCCTAAAGG ATAAAGATCG CCTGAAGAAC TTAGATGAGC AACTCTCTGC TCCAAAGAAA
    GATGTCAAGC AGCCAGAGGA GCTCCCTCCT ATCACAACCA CAACAACTTC TACAACACCA
    GCTACCAGCA CCACCTGTAC AGCCACTGTT CCACCACAGC CACAGTACAG TTACCATGAC
    ATCAATGTCT ATTCCCTCGC AGGCCTGGCA CCACACATTA CTCTAAACCC AACAATTCCC
    TTGTTTCAGG CCCATCCACA GCTGAAGCAG TGTGTGCGCC AAGCAATCGA ACGGGCTGTC
    CAGGAGCTGG TCCATCCTGT GGTGGATCGA TCAATTAAGA TTGCCATGAC TACTTGTGAG
    CAAATAGTCA GGAAAGATTT TGCCCTGGAT TCTGAGGAAT CTCGAATGCG AATAGCAGCT
    CATCACATGA TGCGGAACTT GACAGCTGGA ATGGCTATGA TTACTTGCAG GGAACCTTTA
    CTCATGAGCA TATCAACCAA CCTGAAAAAC AGTTTTGCCT CAGCCCTTCG TACTGCAAGC
    CCACAACAAA GGGAAATGAT GGATCAGGCA GCTGCTCAAT TAGCTCAGGA CAATTGTGAA
    TTAGCTTGCT GTTTTATTCA GAAGACTGCA GTAGAAAAAG CAGGCCCTGA GATGGACAAG
    AGATTAGCAA CTGAATTTGA GCTGAGAAAA CATGCTAGGC AAGAAGGGCG AAGGTATTGT
    GATCCTGTTG TGTTAACATA CCAAGCTGAG CGGATGCCAG AGCAAATCAG GCTGAAAGTT
    GGTGGTGTGG ACCCAAAGCA GCTGGCTGTT TATGAAGAGT TTGCACGCAA TGTGCCTGGC
    TTCTTGCCTA CAAATGATTT AAGTCAGCCC ACAGGATTTT TAGCCCAGCC CATGAAGCAA
    GCTTGGGCAA CAGATGATGT AGCTCAGATT TATGATAAGT GTATTACAGA ACTGGAGCAG
    CATCTTCACG CTATCCCGCC AACTTTGGCC ATGAACCCCC AAGCTCAGGC CCTCAGAAGT
    CTTCTGGAGG TTGTAGTATT ATCACGGAAC TCTCGAGATG CCATTGCTGC TCTTGGATTG
    CTCCAGAAGG CTGTAGAGGG CTTACTGGAT GCCACCAGTG GTGCTGATGC TGACCTCCTT
    CTTCGCTACA GAGAGTGCCA CCTCTTGGTC TTGAAAGCTC TTCAAGATGG CCGGGCATAT
    GGGTCTCCAT GGTGTAACAA ACAGATCACA AGGTGCCTAA TTGAGTGTCG GGATGAATAC
    AAATATAATG TGGAGGCAGT GGAGTTGCTA ATTCGCAATC ATTTGGTCAA CATGCAACAG
    TATGATCTTC ACCTTGCTCA GTCAATGGAG AATGGTTTAA ACTACATGGC TGTGGCATTT
    GCTATGCAAT TGGTAAAAAT ACTGCTGGTG GATGAAAGGA GTGTTGCCCA TGTTACTGAG
    GCAGATCTGT TCCACACCAT TGAAACCCTC ATGAGGATTA ATGCTCACTC CAGAGGCAAT
    GCTCCTGAAG GATTGCCCCA ACTGATGGAA GTAGTGCGAT CCAACTATGA AGCAATGATT
    GATCGTGCTC ATGGAGGCCC TAACTTTATG ATGCATTCTG GGATCTCTCA AGCCTCAGAG
    TATGATGATC CTCCAGGCCT GAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT
    CTCTACCATT CAGCAGCAGC TGGTCGTGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA
    CAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218723.1
    CDS311..7438
    Translation

    Target ORF information:

    RefSeq Version XM_005218666.3
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218666.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGT
    AAAGAGATAG ATGATGAAGC AAACAGCTAT TTCCAGAGAA TATATAATCA CCCACCACAT
    CCAACCATGT CTGTTGATGA GGTATTAGAA ATGCTACAGA GATTTAAAGA TTCTACCATA
    AAGAGGGAAC GAGAAGTATT TAACTGTATG CTAAGGAACT TGTTCGAAGA ATATCGGTTC
    TTCCCCCAAT ACCCTGATAA AGAGTTACAT ATAACAGCCT GCCTGTTTGG TGGAATAATC
    GAAAAAGGAC TGGTCACTTA TATGGCACTA GGTTTGGCCC TGCGATACGT TCTTGAAGCC
    TTACGCAAGC CTTTTGGATC CAAAATGTAT TATTTTGGGA TTGCTGCACT AGATAGATTT
    AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCGAT CAGTCACTTT
    ATGCAATTTC CACATCATTT ACAGGAGTAT ATTGAGTATG GACAGCAATC TAGAGATCCT
    CCTGTGAAAA TGCAGGGCTC TATCACAACC CCTGGAAGTA TTGCACTGGC TCAAGCCCAG
    GCCCAGGCCC AGGTTCCAGC AAAAGCTCCC CTTGCTGGCC AGGTTAGCAC TATGGTAACC
    ACCTCAACAA CCACCACTGT TGCTAAAACG GTTACAGTCA CCAGGCCAAC TGGAGTCAGC
    TTTAAGAAAG ATGTGCCACC TTCTATTAAT ACAACAAATA TAGATACATT GCTTGTGGCC
    ACAGATCAAA CTGAGAGAAT CGTAGAGCCC CCAGAAAATA TCCAGGAGAA AATTGCTTTT
    ATCTTCAACA ACCTTTCCCA GTCAAATATG ACACAAAAGG TTGAAGAACT AAAGGAAACT
    GTGAAGGAAG AATTTATGCC TTGGGTTTCA CAGTACCTGG TTATGAAGAG AGTCAGTATT
    GAGCCAAACT TTCACAGCCT GTATTCTAAC TTCCTTGACA CGCTGAAGAA TCCTGAGTTT
    AATAAGATGG TTCTGAATGA GACCTATAGA AACATCAAAG TACTCCTGAC CTCTGATAAA
    GCTGCAGCCA ATTTCTCTGA TCGGTCTTTG CTGAAGAACT TGGGACATTG GCTAGGAATG
    ATTACACTAG CTAAAAATAA ACCCATCTTA CACACTGACT TGGATGTAAA ATCTTTGCTG
    CTAGAGGCTT ATGTTAAAGG ACAGCAAGAG TTGCTCTATG TAGTGCCCTT TGTTGCCAAA
    GTCTTAGAAT CTAGCATTCG TAGTGTGGTT TTTAGGCCAC CCAACCCCTG GACAATGGCT
    ATTATGAATG TATTAGCTGA GCTACATCAA GAACATGACT TAAAGTTAAA CTTGAAGTTT
    GAAATTGAGG TCCTCTGTAA GAATCTTGCA TTAGACATCA ATGAGCTAAA ACCTGGAAAC
    CTCCTAAAGG ATAAAGATCG CCTGAAGAAC TTAGATGAGC AACTCTCTGC TCCAAAGAAA
    GATGTCAAGC AGCCAGAGGA GCTCCCTCCT ATCACAACCA CAACAACTTC TACAACACCA
    GCTACCAGCA CCACCTGTAC AGCCACTGTT CCACCACAGC CACAGTACAG TTACCATGAC
    ATCAATGTCT ATTCCCTCGC AGGCCTGGCA CCACACATTA CTCTAAACCC AACAATTCCC
    TTGTTTCAGG CCCATCCACA GCTGAAGCAG TGTGTGCGCC AAGCAATCGA ACGGGCTGTC
    CAGGAGCTGG TCCATCCTGT GGTGGATCGA TCAATTAAGA TTGCCATGAC TACTTGTGAG
    CAAATAGTCA GGAAAGATTT TGCCCTGGAT TCTGAGGAAT CTCGAATGCG AATAGCAGCT
    CATCACATGA TGCGGAACTT GACAGCTGGA ATGGCTATGA TTACTTGCAG GGAACCTTTA
    CTCATGAGCA TATCAACCAA CCTGAAAAAC AGTTTTGCCT CAGCCCTTCG TACTGCAAGC
    CCACAACAAA GGGAAATGAT GGATCAGGCA GCTGCTCAAT TAGCTCAGGA CAATTGTGAA
    TTAGCTTGCT GTTTTATTCA GAAGACTGCA GTAGAAAAAG CAGGCCCTGA GATGGACAAG
    AGATTAGCAA CTGAATTTGA GCTGAGAAAA CATGCTAGGC AAGAAGGGCG AAGGTATTGT
    GATCCTGTTG TGTTAACATA CCAAGCTGAG CGGATGCCAG AGCAAATCAG GCTGAAAGTT
    GGTGGTGTGG ACCCAAAGCA GCTGGCTGTT TATGAAGAGT TTGCACGCAA TGTGCCTGGC
    TTCTTGCCTA CAAATGATTT AAGTCAGCCC ACAGGATTTT TAGCCCAGCC CATGAAGCAA
    GCTTGGGCAA CAGATGATGT AGCTCAGATT TATGATAAGT GTATTACAGA ACTGGAGCAG
    CATCTTCACG CTATCCCGCC AACTTTGGCC ATGAACCCCC AAGCTCAGGC CCTCAGAAGT
    CTTCTGGAGG TTGTAGTATT ATCACGGAAC TCTCGAGATG CCATTGCTGC TCTTGGATTG
    CTCCAGAAGG CTGTAGAGGG CTTACTGGAT GCCACCAGTG GTGCTGATGC TGACCTCCTT
    CTTCGCTACA GAGAGTGCCA CCTCTTGGTC TTGAAAGCTC TTCAAGATGG CCGGGCATAT
    GGGTCTCCAT GGTGTAACAA ACAGATCACA AGGTGCCTAA TTGAGTGTCG GGATGAATAC
    AAATATAATG TGGAGGCAGT GGAGTTGCTA ATTCGCAATC ATTTGGTCAA CATGCAACAG
    TATGATCTTC ACCTTGCTCA GTCAATGGAG AATGGTTTAA ACTACATGGC TGTGGCATTT
    GCTATGCAAT TGGTAAAAAT ACTGCTGGTG GATGAAAGGA GTGTTGCCCA TGTTACTGAG
    GCAGATCTGT TCCACACCAT TGAAACCCTC ATGAGGATTA ATGCTCACTC CAGAGGCAAT
    GCTCCTGAAG GATTGCCCCA ACTGATGGAA GTAGTGCGAT CCAACTATGA AGCAATGATT
    GATCGTGCTC ATGGAGGCCC TAACTTTATG ATGCATTCTG GGATCTCTCA AGCCTCAGAG
    TATGATGATC CTCCAGGCCT GAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT
    CTCTACCATT CAGCAGCAGC TGGTCGTGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA
    CAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28467
    Clone ID Related Accession (Same CDS sequence) XM_005218666.3 , XM_005218666.4
    Accession Version XM_005218666.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218666.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGT
    AAAGAGATAG ATGATGAAGC AAACAGCTAT TTCCAGAGAA TATATAATCA CCCACCACAT
    CCAACCATGT CTGTTGATGA GGTATTAGAA ATGCTACAGA GATTTAAAGA TTCTACCATA
    AAGAGGGAAC GAGAAGTATT TAACTGTATG CTAAGGAACT TGTTCGAAGA ATATCGGTTC
    TTCCCCCAAT ACCCTGATAA AGAGTTACAT ATAACAGCCT GCCTGTTTGG TGGAATAATC
    GAAAAAGGAC TGGTCACTTA TATGGCACTA GGTTTGGCCC TGCGATACGT TCTTGAAGCC
    TTACGCAAGC CTTTTGGATC CAAAATGTAT TATTTTGGGA TTGCTGCACT AGATAGATTT
    AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCGAT CAGTCACTTT
    ATGCAATTTC CACATCATTT ACAGGAGTAT ATTGAGTATG GACAGCAATC TAGAGATCCT
    CCTGTGAAAA TGCAGGGCTC TATCACAACC CCTGGAAGTA TTGCACTGGC TCAAGCCCAG
    GCCCAGGCCC AGGTTCCAGC AAAAGCTCCC CTTGCTGGCC AGGTTAGCAC TATGGTAACC
    ACCTCAACAA CCACCACTGT TGCTAAAACG GTTACAGTCA CCAGGCCAAC TGGAGTCAGC
    TTTAAGAAAG ATGTGCCACC TTCTATTAAT ACAACAAATA TAGATACATT GCTTGTGGCC
    ACAGATCAAA CTGAGAGAAT CGTAGAGCCC CCAGAAAATA TCCAGGAGAA AATTGCTTTT
    ATCTTCAACA ACCTTTCCCA GTCAAATATG ACACAAAAGG TTGAAGAACT AAAGGAAACT
    GTGAAGGAAG AATTTATGCC TTGGGTTTCA CAGTACCTGG TTATGAAGAG AGTCAGTATT
    GAGCCAAACT TTCACAGCCT GTATTCTAAC TTCCTTGACA CGCTGAAGAA TCCTGAGTTT
    AATAAGATGG TTCTGAATGA GACCTATAGA AACATCAAAG TACTCCTGAC CTCTGATAAA
    GCTGCAGCCA ATTTCTCTGA TCGGTCTTTG CTGAAGAACT TGGGACATTG GCTAGGAATG
    ATTACACTAG CTAAAAATAA ACCCATCTTA CACACTGACT TGGATGTAAA ATCTTTGCTG
    CTAGAGGCTT ATGTTAAAGG ACAGCAAGAG TTGCTCTATG TAGTGCCCTT TGTTGCCAAA
    GTCTTAGAAT CTAGCATTCG TAGTGTGGTT TTTAGGCCAC CCAACCCCTG GACAATGGCT
    ATTATGAATG TATTAGCTGA GCTACATCAA GAACATGACT TAAAGTTAAA CTTGAAGTTT
    GAAATTGAGG TCCTCTGTAA GAATCTTGCA TTAGACATCA ATGAGCTAAA ACCTGGAAAC
    CTCCTAAAGG ATAAAGATCG CCTGAAGAAC TTAGATGAGC AACTCTCTGC TCCAAAGAAA
    GATGTCAAGC AGCCAGAGGA GCTCCCTCCT ATCACAACCA CAACAACTTC TACAACACCA
    GCTACCAGCA CCACCTGTAC AGCCACTGTT CCACCACAGC CACAGTACAG TTACCATGAC
    ATCAATGTCT ATTCCCTCGC AGGCCTGGCA CCACACATTA CTCTAAACCC AACAATTCCC
    TTGTTTCAGG CCCATCCACA GCTGAAGCAG TGTGTGCGCC AAGCAATCGA ACGGGCTGTC
    CAGGAGCTGG TCCATCCTGT GGTGGATCGA TCAATTAAGA TTGCCATGAC TACTTGTGAG
    CAAATAGTCA GGAAAGATTT TGCCCTGGAT TCTGAGGAAT CTCGAATGCG AATAGCAGCT
    CATCACATGA TGCGGAACTT GACAGCTGGA ATGGCTATGA TTACTTGCAG GGAACCTTTA
    CTCATGAGCA TATCAACCAA CCTGAAAAAC AGTTTTGCCT CAGCCCTTCG TACTGCAAGC
    CCACAACAAA GGGAAATGAT GGATCAGGCA GCTGCTCAAT TAGCTCAGGA CAATTGTGAA
    TTAGCTTGCT GTTTTATTCA GAAGACTGCA GTAGAAAAAG CAGGCCCTGA GATGGACAAG
    AGATTAGCAA CTGAATTTGA GCTGAGAAAA CATGCTAGGC AAGAAGGGCG AAGGTATTGT
    GATCCTGTTG TGTTAACATA CCAAGCTGAG CGGATGCCAG AGCAAATCAG GCTGAAAGTT
    GGTGGTGTGG ACCCAAAGCA GCTGGCTGTT TATGAAGAGT TTGCACGCAA TGTGCCTGGC
    TTCTTGCCTA CAAATGATTT AAGTCAGCCC ACAGGATTTT TAGCCCAGCC CATGAAGCAA
    GCTTGGGCAA CAGATGATGT AGCTCAGATT TATGATAAGT GTATTACAGA ACTGGAGCAG
    CATCTTCACG CTATCCCGCC AACTTTGGCC ATGAACCCCC AAGCTCAGGC CCTCAGAAGT
    CTTCTGGAGG TTGTAGTATT ATCACGGAAC TCTCGAGATG CCATTGCTGC TCTTGGATTG
    CTCCAGAAGG CTGTAGAGGG CTTACTGGAT GCCACCAGTG GTGCTGATGC TGACCTCCTT
    CTTCGCTACA GAGAGTGCCA CCTCTTGGTC TTGAAAGCTC TTCAAGATGG CCGGGCATAT
    GGGTCTCCAT GGTGTAACAA ACAGATCACA AGGTGCCTAA TTGAGTGTCG GGATGAATAC
    AAATATAATG TGGAGGCAGT GGAGTTGCTA ATTCGCAATC ATTTGGTCAA CATGCAACAG
    TATGATCTTC ACCTTGCTCA GTCAATGGAG AATGGTTTAA ACTACATGGC TGTGGCATTT
    GCTATGCAAT TGGTAAAAAT ACTGCTGGTG GATGAAAGGA GTGTTGCCCA TGTTACTGAG
    GCAGATCTGT TCCACACCAT TGAAACCCTC ATGAGGATTA ATGCTCACTC CAGAGGCAAT
    GCTCCTGAAG GATTGCCCCA ACTGATGGAA GTAGTGCGAT CCAACTATGA AGCAATGATT
    GATCGTGCTC ATGGAGGCCC TAACTTTATG ATGCATTCTG GGATCTCTCA AGCCTCAGAG
    TATGATGATC CTCCAGGCCT GAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT
    CTCTACCATT CAGCAGCAGC TGGTCGTGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA
    CAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218723.1
    CDS328..7455
    Translation

    Target ORF information:

    RefSeq Version XM_005218666.4
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218666.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGT
    AAAGAGATAG ATGATGAAGC AAACAGCTAT TTCCAGAGAA TATATAATCA CCCACCACAT
    CCAACCATGT CTGTTGATGA GGTATTAGAA ATGCTACAGA GATTTAAAGA TTCTACCATA
    AAGAGGGAAC GAGAAGTATT TAACTGTATG CTAAGGAACT TGTTCGAAGA ATATCGGTTC
    TTCCCCCAAT ACCCTGATAA AGAGTTACAT ATAACAGCCT GCCTGTTTGG TGGAATAATC
    GAAAAAGGAC TGGTCACTTA TATGGCACTA GGTTTGGCCC TGCGATACGT TCTTGAAGCC
    TTACGCAAGC CTTTTGGATC CAAAATGTAT TATTTTGGGA TTGCTGCACT AGATAGATTT
    AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCGAT CAGTCACTTT
    ATGCAATTTC CACATCATTT ACAGGAGTAT ATTGAGTATG GACAGCAATC TAGAGATCCT
    CCTGTGAAAA TGCAGGGCTC TATCACAACC CCTGGAAGTA TTGCACTGGC TCAAGCCCAG
    GCCCAGGCCC AGGTTCCAGC AAAAGCTCCC CTTGCTGGCC AGGTTAGCAC TATGGTAACC
    ACCTCAACAA CCACCACTGT TGCTAAAACG GTTACAGTCA CCAGGCCAAC TGGAGTCAGC
    TTTAAGAAAG ATGTGCCACC TTCTATTAAT ACAACAAATA TAGATACATT GCTTGTGGCC
    ACAGATCAAA CTGAGAGAAT CGTAGAGCCC CCAGAAAATA TCCAGGAGAA AATTGCTTTT
    ATCTTCAACA ACCTTTCCCA GTCAAATATG ACACAAAAGG TTGAAGAACT AAAGGAAACT
    GTGAAGGAAG AATTTATGCC TTGGGTTTCA CAGTACCTGG TTATGAAGAG AGTCAGTATT
    GAGCCAAACT TTCACAGCCT GTATTCTAAC TTCCTTGACA CGCTGAAGAA TCCTGAGTTT
    AATAAGATGG TTCTGAATGA GACCTATAGA AACATCAAAG TACTCCTGAC CTCTGATAAA
    GCTGCAGCCA ATTTCTCTGA TCGGTCTTTG CTGAAGAACT TGGGACATTG GCTAGGAATG
    ATTACACTAG CTAAAAATAA ACCCATCTTA CACACTGACT TGGATGTAAA ATCTTTGCTG
    CTAGAGGCTT ATGTTAAAGG ACAGCAAGAG TTGCTCTATG TAGTGCCCTT TGTTGCCAAA
    GTCTTAGAAT CTAGCATTCG TAGTGTGGTT TTTAGGCCAC CCAACCCCTG GACAATGGCT
    ATTATGAATG TATTAGCTGA GCTACATCAA GAACATGACT TAAAGTTAAA CTTGAAGTTT
    GAAATTGAGG TCCTCTGTAA GAATCTTGCA TTAGACATCA ATGAGCTAAA ACCTGGAAAC
    CTCCTAAAGG ATAAAGATCG CCTGAAGAAC TTAGATGAGC AACTCTCTGC TCCAAAGAAA
    GATGTCAAGC AGCCAGAGGA GCTCCCTCCT ATCACAACCA CAACAACTTC TACAACACCA
    GCTACCAGCA CCACCTGTAC AGCCACTGTT CCACCACAGC CACAGTACAG TTACCATGAC
    ATCAATGTCT ATTCCCTCGC AGGCCTGGCA CCACACATTA CTCTAAACCC AACAATTCCC
    TTGTTTCAGG CCCATCCACA GCTGAAGCAG TGTGTGCGCC AAGCAATCGA ACGGGCTGTC
    CAGGAGCTGG TCCATCCTGT GGTGGATCGA TCAATTAAGA TTGCCATGAC TACTTGTGAG
    CAAATAGTCA GGAAAGATTT TGCCCTGGAT TCTGAGGAAT CTCGAATGCG AATAGCAGCT
    CATCACATGA TGCGGAACTT GACAGCTGGA ATGGCTATGA TTACTTGCAG GGAACCTTTA
    CTCATGAGCA TATCAACCAA CCTGAAAAAC AGTTTTGCCT CAGCCCTTCG TACTGCAAGC
    CCACAACAAA GGGAAATGAT GGATCAGGCA GCTGCTCAAT TAGCTCAGGA CAATTGTGAA
    TTAGCTTGCT GTTTTATTCA GAAGACTGCA GTAGAAAAAG CAGGCCCTGA GATGGACAAG
    AGATTAGCAA CTGAATTTGA GCTGAGAAAA CATGCTAGGC AAGAAGGGCG AAGGTATTGT
    GATCCTGTTG TGTTAACATA CCAAGCTGAG CGGATGCCAG AGCAAATCAG GCTGAAAGTT
    GGTGGTGTGG ACCCAAAGCA GCTGGCTGTT TATGAAGAGT TTGCACGCAA TGTGCCTGGC
    TTCTTGCCTA CAAATGATTT AAGTCAGCCC ACAGGATTTT TAGCCCAGCC CATGAAGCAA
    GCTTGGGCAA CAGATGATGT AGCTCAGATT TATGATAAGT GTATTACAGA ACTGGAGCAG
    CATCTTCACG CTATCCCGCC AACTTTGGCC ATGAACCCCC AAGCTCAGGC CCTCAGAAGT
    CTTCTGGAGG TTGTAGTATT ATCACGGAAC TCTCGAGATG CCATTGCTGC TCTTGGATTG
    CTCCAGAAGG CTGTAGAGGG CTTACTGGAT GCCACCAGTG GTGCTGATGC TGACCTCCTT
    CTTCGCTACA GAGAGTGCCA CCTCTTGGTC TTGAAAGCTC TTCAAGATGG CCGGGCATAT
    GGGTCTCCAT GGTGTAACAA ACAGATCACA AGGTGCCTAA TTGAGTGTCG GGATGAATAC
    AAATATAATG TGGAGGCAGT GGAGTTGCTA ATTCGCAATC ATTTGGTCAA CATGCAACAG
    TATGATCTTC ACCTTGCTCA GTCAATGGAG AATGGTTTAA ACTACATGGC TGTGGCATTT
    GCTATGCAAT TGGTAAAAAT ACTGCTGGTG GATGAAAGGA GTGTTGCCCA TGTTACTGAG
    GCAGATCTGT TCCACACCAT TGAAACCCTC ATGAGGATTA ATGCTCACTC CAGAGGCAAT
    GCTCCTGAAG GATTGCCCCA ACTGATGGAA GTAGTGCGAT CCAACTATGA AGCAATGATT
    GATCGTGCTC ATGGAGGCCC TAACTTTATG ATGCATTCTG GGATCTCTCA AGCCTCAGAG
    TATGATGATC CTCCAGGCCT GAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT
    CTCTACCATT CAGCAGCAGC TGGTCGTGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA
    CAGATGCACC AGCAAGGAAT CCTGAAGACT GATGACCTCA TAACAAGGTT CTTTCGTCTG
    TGTACTGAAA TGTGTGTTGA AATCAGTTAC CGTGCTCAGG CAGAGCAGCA GCACAATCCT
    GCTGCTAATC CCACCATGAT CCGAGCCAAG TGCTATCACA ACCTGGATGC CTTTGTCCGA
    CTCATTGCAC TGCTAGTAAA GCACTCAGGG GAGGCCACCA ACACTGTCAC AAAGATTAAT
    CTGCTGAATA AGGTCCTCGG TATAGTAGTG GGAGTTCTCC TACAGGATCA TGATGTTCGG
    CAAAGTGAAT TTCAGCAACT TCCCTACCAC CGAATTTTTA TCATGCTGCT CTTGGAACTC
    AATGCACCTG AGCATGTGTT GGAAACCATT AATTTCCAGA CACTTACAGC TTTCTGCAAC
    ACATTCCACA TTTTGAGGCC TACCAAAGCT CCTGGCTTTG TGTATGCTTG GCTTGAACTG
    ATCTCCCATC GGATATTTAT TGCAAGAATG CTGGCACACA CGCCACAGCA GAAGGGATGG
    CCTATGTATG CACAGTTACT GATTGATTTA TTCAAATATT TAGCGCCTTT CCTTAGAAAT
    GTGGAACTCA CCAAACCTAT GCAAATCCTC TACAAGGGCA CTTTAAGGGT GCTGCTGGTT
    CTTTTGCATG ATTTTCCTGA GTTCCTTTGT GATTACCATT ATGGATTTTG TGACGTGATC
    CCACCTAATT GTATCCAGCT AAGAAATCTG ATCCTGAGTG CCTTCCCAAG AAACATGAGG
    CTCCCAGACC CATTCACTCC TAATCTGAAG GTGGATATGT TGAGTGAAAT TAACATCGCT
    CCCCGGATTC TCACCAATTT TACTGGAGTG ATGCCACCTC AATTCAAAAA GGATTTGGAT
    TCCTATCTTA AAACTCGATC ACCTGTCACT TTCCTGTCTG ATCTGCGCAG CAACCTACAG
    GTATCCAATG AGCCTGGTAA TCGCTACAAC CTCCAGCTCA TTAATGCACT GGTGCTCTAT
    GTAGGGACTC AGGCCATTGC ACACATCCAC AACAAGGGCA GCACACCTTC AATGAGCACC
    ATCACGCACT CAGCTCACAT GGACATCTTC CAGAACCTGG CTGTGGATTT GGACACTGAA
    GGTCGGTATC TCTTCTTGAA TGCAATTGCA AATCAGCTGC GGTACCCAAA TAGCCACACT
    CACTACTTCA GCTGCACCAT GCTGTACCTT TTTGCAGAGG CCAACACTGA AGCTATTCAA
    GAACAGATCA CAAGGGTTCT TTTGGAACGG TTGATTGTAA ATAGGCCACA TCCTTGGGGT
    CTTCTTATTA CCTTCATTGA GCTGATTAAA AATCCAGCAT TTAAATTCTG GAACCATGAA
    TTTGTACACT GTGCCCCAGA AATTGAAAAG TTATTCCAGT CCGTCGCACA GTGCTGCATG
    GGACAAAAGC AGGCTCAGCA AGTAATGGAA GGGACAGGTG CCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28468
    Clone ID Related Accession (Same CDS sequence) XM_005218667.4 , XM_005218667.3
    Accession Version XM_005218667.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218667.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GATGCACCAG
    CAAGGAATCC TGAAGACTGA TGACCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG
    TGTGTTGAAA TCAGTTACCG TGCTCAGGCA GAGCAGCAGC ACAATCCTGC TGCTAATCCC
    ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTCCGACT CATTGCACTG
    CTAGTAAAGC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAATAAG
    GTCCTCGGTA TAGTAGTGGG AGTTCTCCTA CAGGATCATG ATGTTCGGCA AAGTGAATTT
    CAGCAACTTC CCTACCACCG AATTTTTATC ATGCTGCTCT TGGAACTCAA TGCACCTGAG
    CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAACAC ATTCCACATT
    TTGAGGCCTA CCAAAGCTCC TGGCTTTGTG TATGCTTGGC TTGAACTGAT CTCCCATCGG
    ATATTTATTG CAAGAATGCT GGCACACACG CCACAGCAGA AGGGATGGCC TATGTATGCA
    CAGTTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC
    AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGGGTGC TGCTGGTTCT TTTGCATGAT
    TTTCCTGAGT TCCTTTGTGA TTACCATTAT GGATTTTGTG ACGTGATCCC ACCTAATTGT
    ATCCAGCTAA GAAATCTGAT CCTGAGTGCC TTCCCAAGAA ACATGAGGCT CCCAGACCCA
    TTCACTCCTA ATCTGAAGGT GGATATGTTG AGTGAAATTA ACATCGCTCC CCGGATTCTC
    ACCAATTTTA CTGGAGTGAT GCCACCTCAA TTCAAAAAGG ATTTGGATTC CTATCTTAAA
    ACTCGATCAC CTGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAG
    CCTGGTAATC GCTACAACCT CCAGCTCATT AATGCACTGG TGCTCTATGT AGGGACTCAG
    GCCATTGCAC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACGCACTCA
    GCTCACATGG ACATCTTCCA GAACCTGGCT GTGGATTTGG ACACTGAAGG TCGGTATCTC
    TTCTTGAATG CAATTGCAAA TCAGCTGCGG TACCCAAATA GCCACACTCA CTACTTCAGC
    TGCACCATGC TGTACCTTTT TGCAGAGGCC AACACTGAAG CTATTCAAGA ACAGATCACA
    AGGGTTCTTT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC
    TTCATTGAGC TGATTAAAAA TCCAGCATTT AAATTCTGGA ACCATGAATT TGTACACTGT
    GCCCCAGAAA TTGAAAAGTT ATTCCAGTCC GTCGCACAGT GCTGCATGGG ACAAAAGCAG
    GCTCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218724.1
    CDS327..7442
    Translation

    Target ORF information:

    RefSeq Version XM_005218667.4
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218667.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GATGCACCAG
    CAAGGAATCC TGAAGACTGA TGACCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG
    TGTGTTGAAA TCAGTTACCG TGCTCAGGCA GAGCAGCAGC ACAATCCTGC TGCTAATCCC
    ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTCCGACT CATTGCACTG
    CTAGTAAAGC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAATAAG
    GTCCTCGGTA TAGTAGTGGG AGTTCTCCTA CAGGATCATG ATGTTCGGCA AAGTGAATTT
    CAGCAACTTC CCTACCACCG AATTTTTATC ATGCTGCTCT TGGAACTCAA TGCACCTGAG
    CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAACAC ATTCCACATT
    TTGAGGCCTA CCAAAGCTCC TGGCTTTGTG TATGCTTGGC TTGAACTGAT CTCCCATCGG
    ATATTTATTG CAAGAATGCT GGCACACACG CCACAGCAGA AGGGATGGCC TATGTATGCA
    CAGTTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC
    AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGGGTGC TGCTGGTTCT TTTGCATGAT
    TTTCCTGAGT TCCTTTGTGA TTACCATTAT GGATTTTGTG ACGTGATCCC ACCTAATTGT
    ATCCAGCTAA GAAATCTGAT CCTGAGTGCC TTCCCAAGAA ACATGAGGCT CCCAGACCCA
    TTCACTCCTA ATCTGAAGGT GGATATGTTG AGTGAAATTA ACATCGCTCC CCGGATTCTC
    ACCAATTTTA CTGGAGTGAT GCCACCTCAA TTCAAAAAGG ATTTGGATTC CTATCTTAAA
    ACTCGATCAC CTGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAG
    CCTGGTAATC GCTACAACCT CCAGCTCATT AATGCACTGG TGCTCTATGT AGGGACTCAG
    GCCATTGCAC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACGCACTCA
    GCTCACATGG ACATCTTCCA GAACCTGGCT GTGGATTTGG ACACTGAAGG TCGGTATCTC
    TTCTTGAATG CAATTGCAAA TCAGCTGCGG TACCCAAATA GCCACACTCA CTACTTCAGC
    TGCACCATGC TGTACCTTTT TGCAGAGGCC AACACTGAAG CTATTCAAGA ACAGATCACA
    AGGGTTCTTT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC
    TTCATTGAGC TGATTAAAAA TCCAGCATTT AAATTCTGGA ACCATGAATT TGTACACTGT
    GCCCCAGAAA TTGAAAAGTT ATTCCAGTCC GTCGCACAGT GCTGCATGGG ACAAAAGCAG
    GCTCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28468
    Clone ID Related Accession (Same CDS sequence) XM_005218667.4 , XM_005218667.3
    Accession Version XM_005218667.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GATGCACCAG
    CAAGGAATCC TGAAGACTGA TGACCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG
    TGTGTTGAAA TCAGTTACCG TGCTCAGGCA GAGCAGCAGC ACAATCCTGC TGCTAATCCC
    ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTCCGACT CATTGCACTG
    CTAGTAAAGC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAATAAG
    GTCCTCGGTA TAGTAGTGGG AGTTCTCCTA CAGGATCATG ATGTTCGGCA AAGTGAATTT
    CAGCAACTTC CCTACCACCG AATTTTTATC ATGCTGCTCT TGGAACTCAA TGCACCTGAG
    CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAACAC ATTCCACATT
    TTGAGGCCTA CCAAAGCTCC TGGCTTTGTG TATGCTTGGC TTGAACTGAT CTCCCATCGG
    ATATTTATTG CAAGAATGCT GGCACACACG CCACAGCAGA AGGGATGGCC TATGTATGCA
    CAGTTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC
    AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGGGTGC TGCTGGTTCT TTTGCATGAT
    TTTCCTGAGT TCCTTTGTGA TTACCATTAT GGATTTTGTG ACGTGATCCC ACCTAATTGT
    ATCCAGCTAA GAAATCTGAT CCTGAGTGCC TTCCCAAGAA ACATGAGGCT CCCAGACCCA
    TTCACTCCTA ATCTGAAGGT GGATATGTTG AGTGAAATTA ACATCGCTCC CCGGATTCTC
    ACCAATTTTA CTGGAGTGAT GCCACCTCAA TTCAAAAAGG ATTTGGATTC CTATCTTAAA
    ACTCGATCAC CTGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAG
    CCTGGTAATC GCTACAACCT CCAGCTCATT AATGCACTGG TGCTCTATGT AGGGACTCAG
    GCCATTGCAC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACGCACTCA
    GCTCACATGG ACATCTTCCA GAACCTGGCT GTGGATTTGG ACACTGAAGG TCGGTATCTC
    TTCTTGAATG CAATTGCAAA TCAGCTGCGG TACCCAAATA GCCACACTCA CTACTTCAGC
    TGCACCATGC TGTACCTTTT TGCAGAGGCC AACACTGAAG CTATTCAAGA ACAGATCACA
    AGGGTTCTTT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC
    TTCATTGAGC TGATTAAAAA TCCAGCATTT AAATTCTGGA ACCATGAATT TGTACACTGT
    GCCCCAGAAA TTGAAAAGTT ATTCCAGTCC GTCGCACAGT GCTGCATGGG ACAAAAGCAG
    GCTCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218724.1
    CDS311..7426
    Translation

    Target ORF information:

    RefSeq Version XM_005218667.3
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218667.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGACGGACT TGTCTCAGGT GTGGCCAGAG GCAAATCAGC ACTTTAGTAA AGAGATAGAT
    GATGAAGCAA ACAGCTATTT CCAGAGAATA TATAATCACC CACCACATCC AACCATGTCT
    GTTGATGAGG TATTAGAAAT GCTACAGAGA TTTAAAGATT CTACCATAAA GAGGGAACGA
    GAAGTATTTA ACTGTATGCT AAGGAACTTG TTCGAAGAAT ATCGGTTCTT CCCCCAATAC
    CCTGATAAAG AGTTACATAT AACAGCCTGC CTGTTTGGTG GAATAATCGA AAAAGGACTG
    GTCACTTATA TGGCACTAGG TTTGGCCCTG CGATACGTTC TTGAAGCCTT ACGCAAGCCT
    TTTGGATCCA AAATGTATTA TTTTGGGATT GCTGCACTAG ATAGATTTAA AAATAGATTG
    AAGGACTATC CCCAGTATTG TCAGCACTTG GCTTCGATCA GTCACTTTAT GCAATTTCCA
    CATCATTTAC AGGAGTATAT TGAGTATGGA CAGCAATCTA GAGATCCTCC TGTGAAAATG
    CAGGGCTCTA TCACAACCCC TGGAAGTATT GCACTGGCTC AAGCCCAGGC CCAGGCCCAG
    GTTCCAGCAA AAGCTCCCCT TGCTGGCCAG GTTAGCACTA TGGTAACCAC CTCAACAACC
    ACCACTGTTG CTAAAACGGT TACAGTCACC AGGCCAACTG GAGTCAGCTT TAAGAAAGAT
    GTGCCACCTT CTATTAATAC AACAAATATA GATACATTGC TTGTGGCCAC AGATCAAACT
    GAGAGAATCG TAGAGCCCCC AGAAAATATC CAGGAGAAAA TTGCTTTTAT CTTCAACAAC
    CTTTCCCAGT CAAATATGAC ACAAAAGGTT GAAGAACTAA AGGAAACTGT GAAGGAAGAA
    TTTATGCCTT GGGTTTCACA GTACCTGGTT ATGAAGAGAG TCAGTATTGA GCCAAACTTT
    CACAGCCTGT ATTCTAACTT CCTTGACACG CTGAAGAATC CTGAGTTTAA TAAGATGGTT
    CTGAATGAGA CCTATAGAAA CATCAAAGTA CTCCTGACCT CTGATAAAGC TGCAGCCAAT
    TTCTCTGATC GGTCTTTGCT GAAGAACTTG GGACATTGGC TAGGAATGAT TACACTAGCT
    AAAAATAAAC CCATCTTACA CACTGACTTG GATGTAAAAT CTTTGCTGCT AGAGGCTTAT
    GTTAAAGGAC AGCAAGAGTT GCTCTATGTA GTGCCCTTTG TTGCCAAAGT CTTAGAATCT
    AGCATTCGTA GTGTGGTTTT TAGGCCACCC AACCCCTGGA CAATGGCTAT TATGAATGTA
    TTAGCTGAGC TACATCAAGA ACATGACTTA AAGTTAAACT TGAAGTTTGA AATTGAGGTC
    CTCTGTAAGA ATCTTGCATT AGACATCAAT GAGCTAAAAC CTGGAAACCT CCTAAAGGAT
    AAAGATCGCC TGAAGAACTT AGATGAGCAA CTCTCTGCTC CAAAGAAAGA TGTCAAGCAG
    CCAGAGGAGC TCCCTCCTAT CACAACCACA ACAACTTCTA CAACACCAGC TACCAGCACC
    ACCTGTACAG CCACTGTTCC ACCACAGCCA CAGTACAGTT ACCATGACAT CAATGTCTAT
    TCCCTCGCAG GCCTGGCACC ACACATTACT CTAAACCCAA CAATTCCCTT GTTTCAGGCC
    CATCCACAGC TGAAGCAGTG TGTGCGCCAA GCAATCGAAC GGGCTGTCCA GGAGCTGGTC
    CATCCTGTGG TGGATCGATC AATTAAGATT GCCATGACTA CTTGTGAGCA AATAGTCAGG
    AAAGATTTTG CCCTGGATTC TGAGGAATCT CGAATGCGAA TAGCAGCTCA TCACATGATG
    CGGAACTTGA CAGCTGGAAT GGCTATGATT ACTTGCAGGG AACCTTTACT CATGAGCATA
    TCAACCAACC TGAAAAACAG TTTTGCCTCA GCCCTTCGTA CTGCAAGCCC ACAACAAAGG
    GAAATGATGG ATCAGGCAGC TGCTCAATTA GCTCAGGACA ATTGTGAATT AGCTTGCTGT
    TTTATTCAGA AGACTGCAGT AGAAAAAGCA GGCCCTGAGA TGGACAAGAG ATTAGCAACT
    GAATTTGAGC TGAGAAAACA TGCTAGGCAA GAAGGGCGAA GGTATTGTGA TCCTGTTGTG
    TTAACATACC AAGCTGAGCG GATGCCAGAG CAAATCAGGC TGAAAGTTGG TGGTGTGGAC
    CCAAAGCAGC TGGCTGTTTA TGAAGAGTTT GCACGCAATG TGCCTGGCTT CTTGCCTACA
    AATGATTTAA GTCAGCCCAC AGGATTTTTA GCCCAGCCCA TGAAGCAAGC TTGGGCAACA
    GATGATGTAG CTCAGATTTA TGATAAGTGT ATTACAGAAC TGGAGCAGCA TCTTCACGCT
    ATCCCGCCAA CTTTGGCCAT GAACCCCCAA GCTCAGGCCC TCAGAAGTCT TCTGGAGGTT
    GTAGTATTAT CACGGAACTC TCGAGATGCC ATTGCTGCTC TTGGATTGCT CCAGAAGGCT
    GTAGAGGGCT TACTGGATGC CACCAGTGGT GCTGATGCTG ACCTCCTTCT TCGCTACAGA
    GAGTGCCACC TCTTGGTCTT GAAAGCTCTT CAAGATGGCC GGGCATATGG GTCTCCATGG
    TGTAACAAAC AGATCACAAG GTGCCTAATT GAGTGTCGGG ATGAATACAA ATATAATGTG
    GAGGCAGTGG AGTTGCTAAT TCGCAATCAT TTGGTCAACA TGCAACAGTA TGATCTTCAC
    CTTGCTCAGT CAATGGAGAA TGGTTTAAAC TACATGGCTG TGGCATTTGC TATGCAATTG
    GTAAAAATAC TGCTGGTGGA TGAAAGGAGT GTTGCCCATG TTACTGAGGC AGATCTGTTC
    CACACCATTG AAACCCTCAT GAGGATTAAT GCTCACTCCA GAGGCAATGC TCCTGAAGGA
    TTGCCCCAAC TGATGGAAGT AGTGCGATCC AACTATGAAG CAATGATTGA TCGTGCTCAT
    GGAGGCCCTA ACTTTATGAT GCATTCTGGG ATCTCTCAAG CCTCAGAGTA TGATGATCCT
    CCAGGCCTGA GGGAGAAGGC AGAGTATCTT CTGAGGGAAT GGGTGAATCT CTACCATTCA
    GCAGCAGCTG GTCGTGACAG TACCAAAGCT TTCTCTGCGT TTGTTGGACA GATGCACCAG
    CAAGGAATCC TGAAGACTGA TGACCTCATA ACAAGGTTCT TTCGTCTGTG TACTGAAATG
    TGTGTTGAAA TCAGTTACCG TGCTCAGGCA GAGCAGCAGC ACAATCCTGC TGCTAATCCC
    ACCATGATCC GAGCCAAGTG CTATCACAAC CTGGATGCCT TTGTCCGACT CATTGCACTG
    CTAGTAAAGC ACTCAGGGGA GGCCACCAAC ACTGTCACAA AGATTAATCT GCTGAATAAG
    GTCCTCGGTA TAGTAGTGGG AGTTCTCCTA CAGGATCATG ATGTTCGGCA AAGTGAATTT
    CAGCAACTTC CCTACCACCG AATTTTTATC ATGCTGCTCT TGGAACTCAA TGCACCTGAG
    CATGTGTTGG AAACCATTAA TTTCCAGACA CTTACAGCTT TCTGCAACAC ATTCCACATT
    TTGAGGCCTA CCAAAGCTCC TGGCTTTGTG TATGCTTGGC TTGAACTGAT CTCCCATCGG
    ATATTTATTG CAAGAATGCT GGCACACACG CCACAGCAGA AGGGATGGCC TATGTATGCA
    CAGTTACTGA TTGATTTATT CAAATATTTA GCGCCTTTCC TTAGAAATGT GGAACTCACC
    AAACCTATGC AAATCCTCTA CAAGGGCACT TTAAGGGTGC TGCTGGTTCT TTTGCATGAT
    TTTCCTGAGT TCCTTTGTGA TTACCATTAT GGATTTTGTG ACGTGATCCC ACCTAATTGT
    ATCCAGCTAA GAAATCTGAT CCTGAGTGCC TTCCCAAGAA ACATGAGGCT CCCAGACCCA
    TTCACTCCTA ATCTGAAGGT GGATATGTTG AGTGAAATTA ACATCGCTCC CCGGATTCTC
    ACCAATTTTA CTGGAGTGAT GCCACCTCAA TTCAAAAAGG ATTTGGATTC CTATCTTAAA
    ACTCGATCAC CTGTCACTTT CCTGTCTGAT CTGCGCAGCA ACCTACAGGT ATCCAATGAG
    CCTGGTAATC GCTACAACCT CCAGCTCATT AATGCACTGG TGCTCTATGT AGGGACTCAG
    GCCATTGCAC ACATCCACAA CAAGGGCAGC ACACCTTCAA TGAGCACCAT CACGCACTCA
    GCTCACATGG ACATCTTCCA GAACCTGGCT GTGGATTTGG ACACTGAAGG TCGGTATCTC
    TTCTTGAATG CAATTGCAAA TCAGCTGCGG TACCCAAATA GCCACACTCA CTACTTCAGC
    TGCACCATGC TGTACCTTTT TGCAGAGGCC AACACTGAAG CTATTCAAGA ACAGATCACA
    AGGGTTCTTT TGGAACGGTT GATTGTAAAT AGGCCACATC CTTGGGGTCT TCTTATTACC
    TTCATTGAGC TGATTAAAAA TCCAGCATTT AAATTCTGGA ACCATGAATT TGTACACTGT
    GCCCCAGAAA TTGAAAAGTT ATTCCAGTCC GTCGCACAGT GCTGCATGGG ACAAAAGCAG
    GCTCAGCAAG TAATGGAAGG GACAGGTGCC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52564
    Clone ID Related Accession (Same CDS sequence) XM_010814532.2 , XM_010814532.3
    Accession Version XM_010814532.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATTGCC CCAACTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG
    ATTGATCGTG CTCATGGAGG CCCTAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA
    GAGTATGATG ATCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG
    AATCTCTACC ATTCAGCAGC AGCTGGTCGT GACAGTACCA AAGCTTTCTC TGCGTTTGTT
    GGACAGGTAG AGCTTTTGGA AAGAAAGATG CACCAGCAAG GAATCCTGAA GACTGATGAC
    CTCATAACAA GGTTCTTTCG TCTGTGTACT GAAATGTGTG TTGAAATCAG TTACCGTGCT
    CAGGCAGAGC AGCAGCACAA TCCTGCTGCT AATCCCACCA TGATCCGAGC CAAGTGCTAT
    CACAACCTGG ATGCCTTTGT CCGACTCATT GCACTGCTAG TAAAGCACTC AGGGGAGGCC
    ACCAACACTG TCACAAAGAT TAATCTGCTG AATAAGGTCC TCGGTATAGT AGTGGGAGTT
    CTCCTACAGG ATCATGATGT TCGGCAAAGT GAATTTCAGC AACTTCCCTA CCACCGAATT
    TTTATCATGC TGCTCTTGGA ACTCAATGCA CCTGAGCATG TGTTGGAAAC CATTAATTTC
    CAGACACTTA CAGCTTTCTG CAACACATTC CACATTTTGA GGCCTACCAA AGCTCCTGGC
    TTTGTGTATG CTTGGCTTGA ACTGATCTCC CATCGGATAT TTATTGCAAG AATGCTGGCA
    CACACGCCAC AGCAGAAGGG ATGGCCTATG TATGCACAGT TACTGATTGA TTTATTCAAA
    TATTTAGCGC CTTTCCTTAG AAATGTGGAA CTCACCAAAC CTATGCAAAT CCTCTACAAG
    GGCACTTTAA GGGTGCTGCT GGTTCTTTTG CATGATTTTC CTGAGTTCCT TTGTGATTAC
    CATTATGGAT TTTGTGACGT GATCCCACCT AATTGTATCC AGCTAAGAAA TCTGATCCTG
    AGTGCCTTCC CAAGAAACAT GAGGCTCCCA GACCCATTCA CTCCTAATCT GAAGGTGGAT
    ATGTTGAGTG AAATTAACAT CGCTCCCCGG ATTCTCACCA ATTTTACTGG AGTGATGCCA
    CCTCAATTCA AAAAGGATTT GGATTCCTAT CTTAAAACTC GATCACCTGT CACTTTCCTG
    TCTGATCTGC GCAGCAACCT ACAGGTATCC AATGAGCCTG GTAATCGCTA CAACCTCCAG
    CTCATTAATG CACTGGTGCT CTATGTAGGG ACTCAGGCCA TTGCACACAT CCACAACAAG
    GGCAGCACAC CTTCAATGAG CACCATCACG CACTCAGCTC ACATGGACAT CTTCCAGAAC
    CTGGCTGTGG ATTTGGACAC TGAAGGTCGG TATCTCTTCT TGAATGCAAT TGCAAATCAG
    CTGCGGTACC CAAATAGCCA CACTCACTAC TTCAGCTGCA CCATGCTGTA CCTTTTTGCA
    GAGGCCAACA CTGAAGCTAT TCAAGAACAG ATCACAAGGG TTCTTTTGGA ACGGTTGATT
    GTAAATAGGC CACATCCTTG GGGTCTTCTT ATTACCTTCA TTGAGCTGAT TAAAAATCCA
    GCATTTAAAT TCTGGAACCA TGAATTTGTA CACTGTGCCC CAGAAATTGA AAAGTTATTC
    CAGTCCGTCG CACAGTGCTG CATGGGACAA AAGCAGGCTC AGCAAGTAAT GGAAGGGACA
    GGTGCCAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812834.1
    CDS311..7462
    Translation

    Target ORF information:

    RefSeq Version XM_010814532.2
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATTGCC CCAACTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG
    ATTGATCGTG CTCATGGAGG CCCTAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA
    GAGTATGATG ATCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG
    AATCTCTACC ATTCAGCAGC AGCTGGTCGT GACAGTACCA AAGCTTTCTC TGCGTTTGTT
    GGACAGGTAG AGCTTTTGGA AAGAAAGATG CACCAGCAAG GAATCCTGAA GACTGATGAC
    CTCATAACAA GGTTCTTTCG TCTGTGTACT GAAATGTGTG TTGAAATCAG TTACCGTGCT
    CAGGCAGAGC AGCAGCACAA TCCTGCTGCT AATCCCACCA TGATCCGAGC CAAGTGCTAT
    CACAACCTGG ATGCCTTTGT CCGACTCATT GCACTGCTAG TAAAGCACTC AGGGGAGGCC
    ACCAACACTG TCACAAAGAT TAATCTGCTG AATAAGGTCC TCGGTATAGT AGTGGGAGTT
    CTCCTACAGG ATCATGATGT TCGGCAAAGT GAATTTCAGC AACTTCCCTA CCACCGAATT
    TTTATCATGC TGCTCTTGGA ACTCAATGCA CCTGAGCATG TGTTGGAAAC CATTAATTTC
    CAGACACTTA CAGCTTTCTG CAACACATTC CACATTTTGA GGCCTACCAA AGCTCCTGGC
    TTTGTGTATG CTTGGCTTGA ACTGATCTCC CATCGGATAT TTATTGCAAG AATGCTGGCA
    CACACGCCAC AGCAGAAGGG ATGGCCTATG TATGCACAGT TACTGATTGA TTTATTCAAA
    TATTTAGCGC CTTTCCTTAG AAATGTGGAA CTCACCAAAC CTATGCAAAT CCTCTACAAG
    GGCACTTTAA GGGTGCTGCT GGTTCTTTTG CATGATTTTC CTGAGTTCCT TTGTGATTAC
    CATTATGGAT TTTGTGACGT GATCCCACCT AATTGTATCC AGCTAAGAAA TCTGATCCTG
    AGTGCCTTCC CAAGAAACAT GAGGCTCCCA GACCCATTCA CTCCTAATCT GAAGGTGGAT
    ATGTTGAGTG AAATTAACAT CGCTCCCCGG ATTCTCACCA ATTTTACTGG AGTGATGCCA
    CCTCAATTCA AAAAGGATTT GGATTCCTAT CTTAAAACTC GATCACCTGT CACTTTCCTG
    TCTGATCTGC GCAGCAACCT ACAGGTATCC AATGAGCCTG GTAATCGCTA CAACCTCCAG
    CTCATTAATG CACTGGTGCT CTATGTAGGG ACTCAGGCCA TTGCACACAT CCACAACAAG
    GGCAGCACAC CTTCAATGAG CACCATCACG CACTCAGCTC ACATGGACAT CTTCCAGAAC
    CTGGCTGTGG ATTTGGACAC TGAAGGTCGG TATCTCTTCT TGAATGCAAT TGCAAATCAG
    CTGCGGTACC CAAATAGCCA CACTCACTAC TTCAGCTGCA CCATGCTGTA CCTTTTTGCA
    GAGGCCAACA CTGAAGCTAT TCAAGAACAG ATCACAAGGG TTCTTTTGGA ACGGTTGATT
    GTAAATAGGC CACATCCTTG GGGTCTTCTT ATTACCTTCA TTGAGCTGAT TAAAAATCCA
    GCATTTAAAT TCTGGAACCA TGAATTTGTA CACTGTGCCC CAGAAATTGA AAAGTTATTC
    CAGTCCGTCG CACAGTGCTG CATGGGACAA AAGCAGGCTC AGCAAGTAAT GGAAGGGACA
    GGTGCCAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52564
    Clone ID Related Accession (Same CDS sequence) XM_010814532.2 , XM_010814532.3
    Accession Version XM_010814532.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814532.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATTGCC CCAACTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG
    ATTGATCGTG CTCATGGAGG CCCTAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA
    GAGTATGATG ATCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG
    AATCTCTACC ATTCAGCAGC AGCTGGTCGT GACAGTACCA AAGCTTTCTC TGCGTTTGTT
    GGACAGGTAG AGCTTTTGGA AAGAAAGATG CACCAGCAAG GAATCCTGAA GACTGATGAC
    CTCATAACAA GGTTCTTTCG TCTGTGTACT GAAATGTGTG TTGAAATCAG TTACCGTGCT
    CAGGCAGAGC AGCAGCACAA TCCTGCTGCT AATCCCACCA TGATCCGAGC CAAGTGCTAT
    CACAACCTGG ATGCCTTTGT CCGACTCATT GCACTGCTAG TAAAGCACTC AGGGGAGGCC
    ACCAACACTG TCACAAAGAT TAATCTGCTG AATAAGGTCC TCGGTATAGT AGTGGGAGTT
    CTCCTACAGG ATCATGATGT TCGGCAAAGT GAATTTCAGC AACTTCCCTA CCACCGAATT
    TTTATCATGC TGCTCTTGGA ACTCAATGCA CCTGAGCATG TGTTGGAAAC CATTAATTTC
    CAGACACTTA CAGCTTTCTG CAACACATTC CACATTTTGA GGCCTACCAA AGCTCCTGGC
    TTTGTGTATG CTTGGCTTGA ACTGATCTCC CATCGGATAT TTATTGCAAG AATGCTGGCA
    CACACGCCAC AGCAGAAGGG ATGGCCTATG TATGCACAGT TACTGATTGA TTTATTCAAA
    TATTTAGCGC CTTTCCTTAG AAATGTGGAA CTCACCAAAC CTATGCAAAT CCTCTACAAG
    GGCACTTTAA GGGTGCTGCT GGTTCTTTTG CATGATTTTC CTGAGTTCCT TTGTGATTAC
    CATTATGGAT TTTGTGACGT GATCCCACCT AATTGTATCC AGCTAAGAAA TCTGATCCTG
    AGTGCCTTCC CAAGAAACAT GAGGCTCCCA GACCCATTCA CTCCTAATCT GAAGGTGGAT
    ATGTTGAGTG AAATTAACAT CGCTCCCCGG ATTCTCACCA ATTTTACTGG AGTGATGCCA
    CCTCAATTCA AAAAGGATTT GGATTCCTAT CTTAAAACTC GATCACCTGT CACTTTCCTG
    TCTGATCTGC GCAGCAACCT ACAGGTATCC AATGAGCCTG GTAATCGCTA CAACCTCCAG
    CTCATTAATG CACTGGTGCT CTATGTAGGG ACTCAGGCCA TTGCACACAT CCACAACAAG
    GGCAGCACAC CTTCAATGAG CACCATCACG CACTCAGCTC ACATGGACAT CTTCCAGAAC
    CTGGCTGTGG ATTTGGACAC TGAAGGTCGG TATCTCTTCT TGAATGCAAT TGCAAATCAG
    CTGCGGTACC CAAATAGCCA CACTCACTAC TTCAGCTGCA CCATGCTGTA CCTTTTTGCA
    GAGGCCAACA CTGAAGCTAT TCAAGAACAG ATCACAAGGG TTCTTTTGGA ACGGTTGATT
    GTAAATAGGC CACATCCTTG GGGTCTTCTT ATTACCTTCA TTGAGCTGAT TAAAAATCCA
    GCATTTAAAT TCTGGAACCA TGAATTTGTA CACTGTGCCC CAGAAATTGA AAAGTTATTC
    CAGTCCGTCG CACAGTGCTG CATGGGACAA AAGCAGGCTC AGCAAGTAAT GGAAGGGACA
    GGTGCCAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812834.1
    CDS334..7485
    Translation

    Target ORF information:

    RefSeq Version XM_010814532.3
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814532.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGTAGGTGGT
    CTTGGCACAG GCAGCCTGAC TGGTATAGGA ACTGGGGCTC TTGGACTCCC TGCAGTGAAT
    AGCGATCCTT TTGTACAGAG GAAACTGGGC ACCTCTGGAC TGAACCAGCC TACATTCCAG
    CAGAGTAAGA TGAAACCTTC GGACTTGTCT CAGGTGTGGC CAGAGGCAAA TCAGCACTTT
    AGTAAAGAGA TAGATGATGA AGCAAACAGC TATTTCCAGA GAATATATAA TCACCCACCA
    CATCCAACCA TGTCTGTTGA TGAGGTATTA GAAATGCTAC AGAGATTTAA AGATTCTACC
    ATAAAGAGGG AACGAGAAGT ATTTAACTGT ATGCTAAGGA ACTTGTTCGA AGAATATCGG
    TTCTTCCCCC AATACCCTGA TAAAGAGTTA CATATAACAG CCTGCCTGTT TGGTGGAATA
    ATCGAAAAAG GACTGGTCAC TTATATGGCA CTAGGTTTGG CCCTGCGATA CGTTCTTGAA
    GCCTTACGCA AGCCTTTTGG ATCCAAAATG TATTATTTTG GGATTGCTGC ACTAGATAGA
    TTTAAAAATA GATTGAAGGA CTATCCCCAG TATTGTCAGC ACTTGGCTTC GATCAGTCAC
    TTTATGCAAT TTCCACATCA TTTACAGGAG TATATTGAGT ATGGACAGCA ATCTAGAGAT
    CCTCCTGTGA AAATGCAGGG CTCTATCACA ACCCCTGGAA GTATTGCACT GGCTCAAGCC
    CAGGCCCAGG CCCAGGTTCC AGCAAAAGCT CCCCTTGCTG GCCAGGTTAG CACTATGGTA
    ACCACCTCAA CAACCACCAC TGTTGCTAAA ACGGTTACAG TCACCAGGCC AACTGGAGTC
    AGCTTTAAGA AAGATGTGCC ACCTTCTATT AATACAACAA ATATAGATAC ATTGCTTGTG
    GCCACAGATC AAACTGAGAG AATCGTAGAG CCCCCAGAAA ATATCCAGGA GAAAATTGCT
    TTTATCTTCA ACAACCTTTC CCAGTCAAAT ATGACACAAA AGGTTGAAGA ACTAAAGGAA
    ACTGTGAAGG AAGAATTTAT GCCTTGGGTT TCACAGTACC TGGTTATGAA GAGAGTCAGT
    ATTGAGCCAA ACTTTCACAG CCTGTATTCT AACTTCCTTG ACACGCTGAA GAATCCTGAG
    TTTAATAAGA TGGTTCTGAA TGAGACCTAT AGAAACATCA AAGTACTCCT GACCTCTGAT
    AAAGCTGCAG CCAATTTCTC TGATCGGTCT TTGCTGAAGA ACTTGGGACA TTGGCTAGGA
    ATGATTACAC TAGCTAAAAA TAAACCCATC TTACACACTG ACTTGGATGT AAAATCTTTG
    CTGCTAGAGG CTTATGTTAA AGGACAGCAA GAGTTGCTCT ATGTAGTGCC CTTTGTTGCC
    AAAGTCTTAG AATCTAGCAT TCGTAGTGTG GTTTTTAGGC CACCCAACCC CTGGACAATG
    GCTATTATGA ATGTATTAGC TGAGCTACAT CAAGAACATG ACTTAAAGTT AAACTTGAAG
    TTTGAAATTG AGGTCCTCTG TAAGAATCTT GCATTAGACA TCAATGAGCT AAAACCTGGA
    AACCTCCTAA AGGATAAAGA TCGCCTGAAG AACTTAGATG AGCAACTCTC TGCTCCAAAG
    AAAGATGTCA AGCAGCCAGA GGAGCTCCCT CCTATCACAA CCACAACAAC TTCTACAACA
    CCAGCTACCA GCACCACCTG TACAGCCACT GTTCCACCAC AGCCACAGTA CAGTTACCAT
    GACATCAATG TCTATTCCCT CGCAGGCCTG GCACCACACA TTACTCTAAA CCCAACAATT
    CCCTTGTTTC AGGCCCATCC ACAGCTGAAG CAGTGTGTGC GCCAAGCAAT CGAACGGGCT
    GTCCAGGAGC TGGTCCATCC TGTGGTGGAT CGATCAATTA AGATTGCCAT GACTACTTGT
    GAGCAAATAG TCAGGAAAGA TTTTGCCCTG GATTCTGAGG AATCTCGAAT GCGAATAGCA
    GCTCATCACA TGATGCGGAA CTTGACAGCT GGAATGGCTA TGATTACTTG CAGGGAACCT
    TTACTCATGA GCATATCAAC CAACCTGAAA AACAGTTTTG CCTCAGCCCT TCGTACTGCA
    AGCCCACAAC AAAGGGAAAT GATGGATCAG GCAGCTGCTC AATTAGCTCA GGACAATTGT
    GAATTAGCTT GCTGTTTTAT TCAGAAGACT GCAGTAGAAA AAGCAGGCCC TGAGATGGAC
    AAGAGATTAG CAACTGAATT TGAGCTGAGA AAACATGCTA GGCAAGAAGG GCGAAGGTAT
    TGTGATCCTG TTGTGTTAAC ATACCAAGCT GAGCGGATGC CAGAGCAAAT CAGGCTGAAA
    GTTGGTGGTG TGGACCCAAA GCAGCTGGCT GTTTATGAAG AGTTTGCACG CAATGTGCCT
    GGCTTCTTGC CTACAAATGA TTTAAGTCAG CCCACAGGAT TTTTAGCCCA GCCCATGAAG
    CAAGCTTGGG CAACAGATGA TGTAGCTCAG ATTTATGATA AGTGTATTAC AGAACTGGAG
    CAGCATCTTC ACGCTATCCC GCCAACTTTG GCCATGAACC CCCAAGCTCA GGCCCTCAGA
    AGTCTTCTGG AGGTTGTAGT ATTATCACGG AACTCTCGAG ATGCCATTGC TGCTCTTGGA
    TTGCTCCAGA AGGCTGTAGA GGGCTTACTG GATGCCACCA GTGGTGCTGA TGCTGACCTC
    CTTCTTCGCT ACAGAGAGTG CCACCTCTTG GTCTTGAAAG CTCTTCAAGA TGGCCGGGCA
    TATGGGTCTC CATGGTGTAA CAAACAGATC ACAAGGTGCC TAATTGAGTG TCGGGATGAA
    TACAAATATA ATGTGGAGGC AGTGGAGTTG CTAATTCGCA ATCATTTGGT CAACATGCAA
    CAGTATGATC TTCACCTTGC TCAGTCAATG GAGAATGGTT TAAACTACAT GGCTGTGGCA
    TTTGCTATGC AATTGGTAAA AATACTGCTG GTGGATGAAA GGAGTGTTGC CCATGTTACT
    GAGGCAGATC TGTTCCACAC CATTGAAACC CTCATGAGGA TTAATGCTCA CTCCAGAGGC
    AATGCTCCTG AAGGATTGCC CCAACTGATG GAAGTAGTGC GATCCAACTA TGAAGCAATG
    ATTGATCGTG CTCATGGAGG CCCTAACTTT ATGATGCATT CTGGGATCTC TCAAGCCTCA
    GAGTATGATG ATCCTCCAGG CCTGAGGGAG AAGGCAGAGT ATCTTCTGAG GGAATGGGTG
    AATCTCTACC ATTCAGCAGC AGCTGGTCGT GACAGTACCA AAGCTTTCTC TGCGTTTGTT
    GGACAGGTAG AGCTTTTGGA AAGAAAGATG CACCAGCAAG GAATCCTGAA GACTGATGAC
    CTCATAACAA GGTTCTTTCG TCTGTGTACT GAAATGTGTG TTGAAATCAG TTACCGTGCT
    CAGGCAGAGC AGCAGCACAA TCCTGCTGCT AATCCCACCA TGATCCGAGC CAAGTGCTAT
    CACAACCTGG ATGCCTTTGT CCGACTCATT GCACTGCTAG TAAAGCACTC AGGGGAGGCC
    ACCAACACTG TCACAAAGAT TAATCTGCTG AATAAGGTCC TCGGTATAGT AGTGGGAGTT
    CTCCTACAGG ATCATGATGT TCGGCAAAGT GAATTTCAGC AACTTCCCTA CCACCGAATT
    TTTATCATGC TGCTCTTGGA ACTCAATGCA CCTGAGCATG TGTTGGAAAC CATTAATTTC
    CAGACACTTA CAGCTTTCTG CAACACATTC CACATTTTGA GGCCTACCAA AGCTCCTGGC
    TTTGTGTATG CTTGGCTTGA ACTGATCTCC CATCGGATAT TTATTGCAAG AATGCTGGCA
    CACACGCCAC AGCAGAAGGG ATGGCCTATG TATGCACAGT TACTGATTGA TTTATTCAAA
    TATTTAGCGC CTTTCCTTAG AAATGTGGAA CTCACCAAAC CTATGCAAAT CCTCTACAAG
    GGCACTTTAA GGGTGCTGCT GGTTCTTTTG CATGATTTTC CTGAGTTCCT TTGTGATTAC
    CATTATGGAT TTTGTGACGT GATCCCACCT AATTGTATCC AGCTAAGAAA TCTGATCCTG
    AGTGCCTTCC CAAGAAACAT GAGGCTCCCA GACCCATTCA CTCCTAATCT GAAGGTGGAT
    ATGTTGAGTG AAATTAACAT CGCTCCCCGG ATTCTCACCA ATTTTACTGG AGTGATGCCA
    CCTCAATTCA AAAAGGATTT GGATTCCTAT CTTAAAACTC GATCACCTGT CACTTTCCTG
    TCTGATCTGC GCAGCAACCT ACAGGTATCC AATGAGCCTG GTAATCGCTA CAACCTCCAG
    CTCATTAATG CACTGGTGCT CTATGTAGGG ACTCAGGCCA TTGCACACAT CCACAACAAG
    GGCAGCACAC CTTCAATGAG CACCATCACG CACTCAGCTC ACATGGACAT CTTCCAGAAC
    CTGGCTGTGG ATTTGGACAC TGAAGGTCGG TATCTCTTCT TGAATGCAAT TGCAAATCAG
    CTGCGGTACC CAAATAGCCA CACTCACTAC TTCAGCTGCA CCATGCTGTA CCTTTTTGCA
    GAGGCCAACA CTGAAGCTAT TCAAGAACAG ATCACAAGGG TTCTTTTGGA ACGGTTGATT
    GTAAATAGGC CACATCCTTG GGGTCTTCTT ATTACCTTCA TTGAGCTGAT TAAAAATCCA
    GCATTTAAAT TCTGGAACCA TGAATTTGTA CACTGTGCCC CAGAAATTGA AAAGTTATTC
    CAGTCCGTCG CACAGTGCTG CATGGGACAA AAGCAGGCTC AGCAAGTAAT GGAAGGGACA
    GGTGCCAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52565
    Clone ID Related Accession (Same CDS sequence) XM_010814533.2
    Accession Version XM_010814533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product CCR4-NOT transcription complex subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGT
    AAAGAGATAG ATGATGAAGC AAACAGCTAT TTCCAGAGAA TATATAATCA CCCACCACAT
    CCAACCATGT CTGTTGATGA GGTATTAGAA ATGCTACAGA GATTTAAAGA TTCTACCATA
    AAGAGGGAAC GAGAAGTATT TAACTGTATG CTAAGGAACT TGTTCGAAGA ATATCGGTTC
    TTCCCCCAAT ACCCTGATAA AGAGTTACAT ATAACAGCCT GCCTGTTTGG TGGAATAATC
    GAAAAAGGAC TGGTCACTTA TATGGCACTA GGTTTGGCCC TGCGATACGT TCTTGAAGCC
    TTACGCAAGC CTTTTGGATC CAAAATGTAT TATTTTGGGA TTGCTGCACT AGATAGATTT
    AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCGAT CAGTCACTTT
    ATGCAATTTC CACATCATTT ACAGGAGTAT ATTGAGTATG GACAGCAATC TAGAGATCCT
    CCTGTGAAAA TGCAGGGCTC TATCACAACC CCTGGAAGTA TTGCACTGGC TCAAGCCCAG
    GCCCAGGCCC AGGTTCCAGC AAAAGCTCCC CTTGCTGGCC AGGTTAGCAC TATGGTAACC
    ACCTCAACAA CCACCACTGT TGCTAAAACG GTTACAGTCA CCAGGCCAAC TGGAGTCAGC
    TTTAAGAAAG ATGTGCCACC TTCTATTAAT ACAACAAATA TAGATACATT GCTTGTGGCC
    ACAGATCAAA CTGAGAGAAT CGTAGAGCCC CCAGAAAATA TCCAGGAGAA AATTGCTTTT
    ATCTTCAACA ACCTTTCCCA GTCAAATATG ACACAAAAGG TTGAAGAACT AAAGGAAACT
    GTGAAGGAAG AATTTATGCC TTGGGTTTCA CAGTACCTGG TTATGAAGAG AGTCAGTATT
    GAGCCAAACT TTCACAGCCT GTATTCTAAC TTCCTTGACA CGCTGAAGAA TCCTGAGTTT
    AATAAGATGG TTCTGAATGA GACCTATAGA AACATCAAAG TACTCCTGAC CTCTGATAAA
    GCTGCAGCCA ATTTCTCTGA TCGGTCTTTG CTGAAGAACT TGGGACATTG GCTAGGAATG
    ATTACACTAG CTAAAAATAA ACCCATCTTA CACACTGACT TGGATGTAAA ATCTTTGCTG
    CTAGAGGCTT ATGTTAAAGG ACAGCAAGAG TTGCTCTATG TAGTGCCCTT TGTTGCCAAA
    GTCTTAGAAT CTAGCATTCG TAGTGTGGTT TTTAGGCCAC CCAACCCCTG GACAATGGCT
    ATTATGAATG TATTAGCTGA GCTACATCAA GAACATGACT TAAAGTTAAA CTTGAAGTTT
    GAAATTGAGG TCCTCTGTAA GAATCTTGCA TTAGACATCA ATGAGCTAAA ACCTGGAAAC
    CTCCTAAAGG ATAAAGATCG CCTGAAGAAC TTAGATGAGC AACTCTCTGC TCCAAAGAAA
    GATGTCAAGC AGCCAGAGGA GCTCCCTCCT ATCACAACCA CAACAACTTC TACAACACCA
    GCTACCAGCA CCACCTGTAC AGCCACTGTT CCACCACAGC CACAGTACAG TTACCATGAC
    ATCAATGTCT ATTCCCTCGC AGGCCTGGCA CCACACATTA CTCTAAACCC AACAATTCCC
    TTGTTTCAGG CCCATCCACA GCTGAAGCAG TGTGTGCGCC AAGCAATCGA ACGGGCTGTC
    CAGGAGCTGG TCCATCCTGT GGTGGATCGA TCAATTAAGA TTGCCATGAC TACTTGTGAG
    CAAATAGTCA GGAAAGATTT TGCCCTGGAT TCTGAGGAAT CTCGAATGCG AATAGCAGCT
    CATCACATGA TGCGGAACTT GACAGCTGGA ATGGCTATGA TTACTTGCAG GGAACCTTTA
    CTCATGAGCA TATCAACCAA CCTGAAAAAC AGTTTTGCCT CAGCCCTTCG TACTGCAAGC
    CCACAACAAA GGGAAATGAT GGATCAGGCA GCTGCTCAAT TAGCTCAGGA CAATTGTGAA
    TTAGCTTGCT GTTTTATTCA GAAGACTGCA GTAGAAAAAG CAGGCCCTGA GATGGACAAG
    AGATTAGCAA CTGAATTTGA GCTGAGAAAA CATGCTAGGC AAGAAGGGCG AAGGTATTGT
    GATCCTGTTG TGTTAACATA CCAAGCTGAG CGGATGCCAG AGCAAATCAG GCTGAAAGTT
    GGTGGTGTGG ACCCAAAGCA GCTGGCTGTT TATGAAGAGT TTGCACGCAA TGTGCCTGGC
    TTCTTGCCTA CAAATGATTT AAGTCAGCCC ACAGGATTTT TAGCCCAGCC CATGAAGCAA
    GCTTGGGCAA CAGATGATGT AGCTCAGATT TATGATAAGT GTATTACAGA ACTGGAGCAG
    CATCTTCACG CTATCCCGCC AACTTTGGCC ATGAACCCCC AAGCTCAGGC CCTCAGAAGT
    CTTCTGGAGG TTGTAGTATT ATCACGGAAC TCTCGAGATG CCATTGCTGC TCTTGGATTG
    CTCCAGAAGG CTGTAGAGGG CTTACTGGAT GCCACCAGTG GTGCTGATGC TGACCTCCTT
    CTTCGCTACA GAGAGTGCCA CCTCTTGGTC TTGAAAGCTC TTCAAGATGG CCGGGCATAT
    GGGTCTCCAT GGTGTAACAA ACAGATCACA AGGTGCCTAA TTGAGTGTCG GGATGAATAC
    AAATATAATG TGGAGGCAGT GGAGTTGCTA ATTCGCAATC ATTTGGTCAA CATGCAACAG
    TATGATCTTC ACCTTGCTCA GTCAATGGAG AATGGTTTAA ACTACATGGC TGTGGCATTT
    GCTATGCAAT TGGTAAAAAT ACTGCTGGTG GATGAAAGGA GTGTTGCCCA TGTTACTGAG
    GCAGATCTGT TCCACACCAT TGAAACCCTC ATGAGGATTA ATGCTCACTC CAGAGGCAAT
    GCTCCTGAAG GATTGCCCCA ACTGATGGAA GTAGTGCGAT CCAACTATGA AGCAATGATT
    GATCGTGCTC ATGGAGGCCC TAACTTTATG ATGCATTCTG GGATCTCTCA AGCCTCAGAG
    TATGATGATC CTCCAGGCCT GAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT
    CTCTACCATT CAGCAGCAGC TGGTCGTGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA
    CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCCTGAAGAC TGATGACCTC
    ATAACAAGGT TCTTTCGTCT GTGTACTGAA ATGTGTGTTG AAATCAGTTA CCGTGCTCAG
    GCAGAGCAGC AGCACAATCC TGCTGCTAAT CCCACCATGA TCCGAGCCAA GTGCTATCAC
    AACCTGGATG CCTTTGTCCG ACTCATTGCA CTGCTAGTAA AGCACTCAGG GGAGGCCACC
    AACACTGTCA CAAAGATTAA TCTGCTGAAT AAGGTCCTCG GTATAGTAGT GGGAGTTCTC
    CTACAGGATC ATGATGTTCG GCAAAGTGAA TTTCAGCAAC TTCCCTACCA CCGAATTTTT
    ATCATGCTGC TCTTGGAACT CAATGCACCT GAGCATGTGT TGGAAACCAT TAATTTCCAG
    ACACTTACAG CTTTCTGCAA CACATTCCAC ATTTTGAGGC CTACCAAAGC TCCTGGCTTT
    GTGTATGCTT GGCTTGAACT GATCTCCCAT CGGATATTTA TTGCAAGAAT GCTGGCACAC
    ACGCCACAGC AGAAGGGATG GCCTATGTAT GCACAGTTAC TGATTGATTT ATTCAAATAT
    TTAGCGCCTT TCCTTAGAAA TGTGGAACTC ACCAAACCTA TGCAAATCCT CTACAAGGGC
    ACTTTAAGGG TGCTGCTGGT TCTTTTGCAT GATTTTCCTG AGTTCCTTTG TGATTACCAT
    TATGGATTTT GTGACGTGAT CCCACCTAAT TGTATCCAGC TAAGAAATCT GATCCTGAGT
    GCCTTCCCAA GAAACATGAG GCTCCCAGAC CCATTCACTC CTAATCTGAA GGTGGATATG
    TTGAGTGAAA TTAACATCGC TCCCCGGATT CTCACCAATT TTACTGGAGT GATGCCACCT
    CAATTCAAAA AGGATTTGGA TTCCTATCTT AAAACTCGAT CACCTGTCAC TTTCCTGTCT
    GATCTGCGCA GCAACCTACA GGTATCCAAT GAGCCTGGTA ATCGCTACAA CCTCCAGCTC
    ATTAATGCAC TGGTGCTCTA TGTAGGGACT CAGGCCATTG CACACATCCA CAACAAGGGC
    AGCACACCTT CAATGAGCAC CATCACGCAC TCAGCTCACA TGGACATCTT CCAGAACCTG
    GCTGTGGATT TGGACACTGA AGGTCGGTAT CTCTTCTTGA ATGCAATTGC AAATCAGCTG
    CGGTACCCAA ATAGCCACAC TCACTACTTC AGCTGCACCA TGCTGTACCT TTTTGCAGAG
    GCCAACACTG AAGCTATTCA AGAACAGATC ACAAGGGTTC TTTTGGAACG GTTGATTGTA
    AATAGGCCAC ATCCTTGGGG TCTTCTTATT ACCTTCATTG AGCTGATTAA AAATCCAGCA
    TTTAAATTCT GGAACCATGA ATTTGTACAC TGTGCCCCAG AAATTGAAAA GTTATTCCAG
    TCCGTCGCAC AGTGCTGCAT GGGACAAAAG CAGGCTCAGC AAGTAATGGA AGGGACAGGT
    GCCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812835.1
    CDS311..7459
    Translation

    Target ORF information:

    RefSeq Version XM_010814533.2
    Organism Bos taurus(cattle)
    Definition Bos taurus CCR4-NOT transcription complex subunit 1 (CNOT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA 
    ACCAAGAAAA ACTACCGAGC CAGCCAGCAG GAAATACAGC ATATTGTGAA TCGGCACGGT
    CCTGAGGCAG ACAGGCATTT ATTACGCTGC CTATTTTCGC ATGTGGATTT CAGTGGCGAT
    GGTAAAAGTA GTGGCAAAGA TTTTCATCAG ACTCAGTTTC TGATTCAGGA GTGTGCGTCA
    CTGATTACGA AGCCAAATTT TATCTCGACG CTGTCCTATG CCATTGATAA TCCATTGCAC
    TATCAGAAGA GTTTAAAGCC TGCACCCCAC TTATTTGCCC AGCTGAGTAA AGTTCTCAAG
    TTAAGCAAAG TACAAGAGGT AATTTTTGGC CTTGCCCTCT TGAATTCTTC CAGCACAGAT
    CTCAGAGGTT TTGCTGCCCA GTTTATCAAA CAGAAGCTTC CAGATCTTCT GCGTTCTTAC
    ATTGACGCAG ACGTCAGTGG AAATCAGGAA GGTGGCTTCC AAGATATAGC AATAGAGGTC
    CTACACCTCC TCCTGTCCCA TCTCCTCTTT GGGCAGAAGG GAGCCTTTGG AGTTGGACAG
    GAACAGATAG ACGCTTTTCT TAAGACGCTG CGCAGAGATT TTCCCCAGGA ACGCTGTCCT
    GTGGTGCTTG CACCACTTTT ATACCCTGAA AAACGGGACA TTCTAATGGA CAGAATCCTG
    CCTGATTCCG GAGGGGTAGC TAAAACCATG ATGGAGAGCT CTTTGGCTGA TTTTATGCAA
    GAAGTGGGCT ATGGCTTTTG TGCAAGTATT GAAGAATGTC GCAATATAAT CATGCAGTTT
    GGTGTTCGGG AGGTCACAGC TGCCCAGGTT GCAAGGGTTT TAGGAATGAT GGCTCGAACT
    CATTCAGGAT TAACAGACGG AATTCCATTA CAGAGTATCT CTGCTCCTGG CAGTGGAATC
    TGGAGTGATG GGAAAGATAA AAGTGATGGA GCACAGGCAC ACACATGGAA TGTAGAAGTC
    TTGATCGATG TTCTCAAAGA ACTGAACCCG AGTTTGAATT TCAAGGAAGT AACTTATGAA
    CTGGACCATC CTGGGTTTCA AATTCGTGAT AGTAAAGGAC TTCATAATGT AGTCTATGGC
    ATTCAAAGGG GTCTGGGTAT GGAAGTGTTC CCTGTAGATC TCATATACAG ACCTTGGAAA
    CACGCAGAAG GCCAGCTCTC CTTCATTCAG CATTCCCTTA TAAATCCAGA GATCTTCTGT
    TTTGCCGACT ATCCCTGTCA TACTGTTGCC ACTGATATTC TGAAAGCACC ACCAGAGGAT
    GACAATCGAG AAATTGCCAC ATGGAAGAGC TTGGATTTGA TTGAATCTCT GCTAAGGCTC
    GCAGAGGTAG GGCAATATGA GCAAGTCAAA CAGCTCTTCA GCTTCCCTAT CAAACACTGT
    CCAGACATGC TGGTATTGGC CTTATTACAA ATCAACACCT CCTGGCACAC CTTGCGCCAC
    GAACTCATCT CCACTCTGAT GCCAATTTTC CTTGGAAACC ACCCTAACTC AGCTATAATT
    TTGCACTATG CATGGCATGG ACAGGGACAG TCTCCCTCAA TCCGCCAACT TATCATGCAT
    GCAATGGCAG AATGGTACAT GCGAGGGGAG CAGTATGACC AGGCCAAGTT GTCTCGAATA
    CTTGATGTGG CCCAGGACTT GAAGGCTTTG TCAATGCTGC TAAATGGTAC TCCATTTGCC
    TTTGTTATTG ACCTTGCTGC ACTTGCTTCA CGTCGTGAAT ACCTCAAACT TGACAAATGG
    CTCACAGATA AAATTCGAGA GCATGGAGAG CCTTTTATCC AGGCTTGTAT GACTTTTTTA
    AAGAGACGGT GTCCTTCTAT TTTGGGGGGA CTTGCCCCAG AGAAAGATCA GCCTAAAAGT
    GCTCAACTTC CTCCAGAAAC CCTCGCGACA ATGTTGGCCT GTTTACAAGC TTGTGCAGGG
    AGTGTTTCTC AGGAGTTATC AGAAACCATC CTCACCATGG TGGCCAATTG CAGTAACGTT
    ATGAACAAGG CCAGGCAGCC ACCACCGGGA GTTATGCCAA AAGGACGCCC TCCTAGTGCT
    AGCAGCTTGG ACGCCATTTC TCCCGTTCAG ATTGACCCTC TTGCTGGAAT GGCATCTCTT
    AGTATAGGTG GTTCAGCTGC CCCTCACACC CAGAGTATGC AGGGCTTTCC TCCAAATTTG
    GGTTCTGCAT TCAGTACCCC ACAGTCACCA GCAAAAGCAT TTCCACCCCT TTCAACCCCA
    AATCAGACAA CTGCATTCAG TGGTATTGGA GGACTTTCAT CACAGCTTCC AGGTGGTCTT
    GGCACAGGCA GCCTGACTGG TATAGGAACT GGGGCTCTTG GACTCCCTGC AGTGAATAGC
    GATCCTTTTG TACAGAGGAA ACTGGGCACC TCTGGACTGA ACCAGCCTAC ATTCCAGCAG
    AGTAAGATGA AACCTTCGGA CTTGTCTCAG GTGTGGCCAG AGGCAAATCA GCACTTTAGT
    AAAGAGATAG ATGATGAAGC AAACAGCTAT TTCCAGAGAA TATATAATCA CCCACCACAT
    CCAACCATGT CTGTTGATGA GGTATTAGAA ATGCTACAGA GATTTAAAGA TTCTACCATA
    AAGAGGGAAC GAGAAGTATT TAACTGTATG CTAAGGAACT TGTTCGAAGA ATATCGGTTC
    TTCCCCCAAT ACCCTGATAA AGAGTTACAT ATAACAGCCT GCCTGTTTGG TGGAATAATC
    GAAAAAGGAC TGGTCACTTA TATGGCACTA GGTTTGGCCC TGCGATACGT TCTTGAAGCC
    TTACGCAAGC CTTTTGGATC CAAAATGTAT TATTTTGGGA TTGCTGCACT AGATAGATTT
    AAAAATAGAT TGAAGGACTA TCCCCAGTAT TGTCAGCACT TGGCTTCGAT CAGTCACTTT
    ATGCAATTTC CACATCATTT ACAGGAGTAT ATTGAGTATG GACAGCAATC TAGAGATCCT
    CCTGTGAAAA TGCAGGGCTC TATCACAACC CCTGGAAGTA TTGCACTGGC TCAAGCCCAG
    GCCCAGGCCC AGGTTCCAGC AAAAGCTCCC CTTGCTGGCC AGGTTAGCAC TATGGTAACC
    ACCTCAACAA CCACCACTGT TGCTAAAACG GTTACAGTCA CCAGGCCAAC TGGAGTCAGC
    TTTAAGAAAG ATGTGCCACC TTCTATTAAT ACAACAAATA TAGATACATT GCTTGTGGCC
    ACAGATCAAA CTGAGAGAAT CGTAGAGCCC CCAGAAAATA TCCAGGAGAA AATTGCTTTT
    ATCTTCAACA ACCTTTCCCA GTCAAATATG ACACAAAAGG TTGAAGAACT AAAGGAAACT
    GTGAAGGAAG AATTTATGCC TTGGGTTTCA CAGTACCTGG TTATGAAGAG AGTCAGTATT
    GAGCCAAACT TTCACAGCCT GTATTCTAAC TTCCTTGACA CGCTGAAGAA TCCTGAGTTT
    AATAAGATGG TTCTGAATGA GACCTATAGA AACATCAAAG TACTCCTGAC CTCTGATAAA
    GCTGCAGCCA ATTTCTCTGA TCGGTCTTTG CTGAAGAACT TGGGACATTG GCTAGGAATG
    ATTACACTAG CTAAAAATAA ACCCATCTTA CACACTGACT TGGATGTAAA ATCTTTGCTG
    CTAGAGGCTT ATGTTAAAGG ACAGCAAGAG TTGCTCTATG TAGTGCCCTT TGTTGCCAAA
    GTCTTAGAAT CTAGCATTCG TAGTGTGGTT TTTAGGCCAC CCAACCCCTG GACAATGGCT
    ATTATGAATG TATTAGCTGA GCTACATCAA GAACATGACT TAAAGTTAAA CTTGAAGTTT
    GAAATTGAGG TCCTCTGTAA GAATCTTGCA TTAGACATCA ATGAGCTAAA ACCTGGAAAC
    CTCCTAAAGG ATAAAGATCG CCTGAAGAAC TTAGATGAGC AACTCTCTGC TCCAAAGAAA
    GATGTCAAGC AGCCAGAGGA GCTCCCTCCT ATCACAACCA CAACAACTTC TACAACACCA
    GCTACCAGCA CCACCTGTAC AGCCACTGTT CCACCACAGC CACAGTACAG TTACCATGAC
    ATCAATGTCT ATTCCCTCGC AGGCCTGGCA CCACACATTA CTCTAAACCC AACAATTCCC
    TTGTTTCAGG CCCATCCACA GCTGAAGCAG TGTGTGCGCC AAGCAATCGA ACGGGCTGTC
    CAGGAGCTGG TCCATCCTGT GGTGGATCGA TCAATTAAGA TTGCCATGAC TACTTGTGAG
    CAAATAGTCA GGAAAGATTT TGCCCTGGAT TCTGAGGAAT CTCGAATGCG AATAGCAGCT
    CATCACATGA TGCGGAACTT GACAGCTGGA ATGGCTATGA TTACTTGCAG GGAACCTTTA
    CTCATGAGCA TATCAACCAA CCTGAAAAAC AGTTTTGCCT CAGCCCTTCG TACTGCAAGC
    CCACAACAAA GGGAAATGAT GGATCAGGCA GCTGCTCAAT TAGCTCAGGA CAATTGTGAA
    TTAGCTTGCT GTTTTATTCA GAAGACTGCA GTAGAAAAAG CAGGCCCTGA GATGGACAAG
    AGATTAGCAA CTGAATTTGA GCTGAGAAAA CATGCTAGGC AAGAAGGGCG AAGGTATTGT
    GATCCTGTTG TGTTAACATA CCAAGCTGAG CGGATGCCAG AGCAAATCAG GCTGAAAGTT
    GGTGGTGTGG ACCCAAAGCA GCTGGCTGTT TATGAAGAGT TTGCACGCAA TGTGCCTGGC
    TTCTTGCCTA CAAATGATTT AAGTCAGCCC ACAGGATTTT TAGCCCAGCC CATGAAGCAA
    GCTTGGGCAA CAGATGATGT AGCTCAGATT TATGATAAGT GTATTACAGA ACTGGAGCAG
    CATCTTCACG CTATCCCGCC AACTTTGGCC ATGAACCCCC AAGCTCAGGC CCTCAGAAGT
    CTTCTGGAGG TTGTAGTATT ATCACGGAAC TCTCGAGATG CCATTGCTGC TCTTGGATTG
    CTCCAGAAGG CTGTAGAGGG CTTACTGGAT GCCACCAGTG GTGCTGATGC TGACCTCCTT
    CTTCGCTACA GAGAGTGCCA CCTCTTGGTC TTGAAAGCTC TTCAAGATGG CCGGGCATAT
    GGGTCTCCAT GGTGTAACAA ACAGATCACA AGGTGCCTAA TTGAGTGTCG GGATGAATAC
    AAATATAATG TGGAGGCAGT GGAGTTGCTA ATTCGCAATC ATTTGGTCAA CATGCAACAG
    TATGATCTTC ACCTTGCTCA GTCAATGGAG AATGGTTTAA ACTACATGGC TGTGGCATTT
    GCTATGCAAT TGGTAAAAAT ACTGCTGGTG GATGAAAGGA GTGTTGCCCA TGTTACTGAG
    GCAGATCTGT TCCACACCAT TGAAACCCTC ATGAGGATTA ATGCTCACTC CAGAGGCAAT
    GCTCCTGAAG GATTGCCCCA ACTGATGGAA GTAGTGCGAT CCAACTATGA AGCAATGATT
    GATCGTGCTC ATGGAGGCCC TAACTTTATG ATGCATTCTG GGATCTCTCA AGCCTCAGAG
    TATGATGATC CTCCAGGCCT GAGGGAGAAG GCAGAGTATC TTCTGAGGGA ATGGGTGAAT
    CTCTACCATT CAGCAGCAGC TGGTCGTGAC AGTACCAAAG CTTTCTCTGC GTTTGTTGGA
    CAGGTAGAGC TTTTGGAAAG AAAGATGCAC CAGCAAGGAA TCCTGAAGAC TGATGACCTC
    ATAACAAGGT TCTTTCGTCT GTGTACTGAA ATGTGTGTTG AAATCAGTTA CCGTGCTCAG
    GCAGAGCAGC AGCACAATCC TGCTGCTAAT CCCACCATGA TCCGAGCCAA GTGCTATCAC
    AACCTGGATG CCTTTGTCCG ACTCATTGCA CTGCTAGTAA AGCACTCAGG GGAGGCCACC
    AACACTGTCA CAAAGATTAA TCTGCTGAAT AAGGTCCTCG GTATAGTAGT GGGAGTTCTC
    CTACAGGATC ATGATGTTCG GCAAAGTGAA TTTCAGCAAC TTCCCTACCA CCGAATTTTT
    ATCATGCTGC TCTTGGAACT CAATGCACCT GAGCATGTGT TGGAAACCAT TAATTTCCAG
    ACACTTACAG CTTTCTGCAA CACATTCCAC ATTTTGAGGC CTACCAAAGC TCCTGGCTTT
    GTGTATGCTT GGCTTGAACT GATCTCCCAT CGGATATTTA TTGCAAGAAT GCTGGCACAC
    ACGCCACAGC AGAAGGGATG GCCTATGTAT GCACAGTTAC TGATTGATTT ATTCAAATAT
    TTAGCGCCTT TCCTTAGAAA TGTGGAACTC ACCAAACCTA TGCAAATCCT CTACAAGGGC
    ACTTTAAGGG TGCTGCTGGT TCTTTTGCAT GATTTTCCTG AGTTCCTTTG TGATTACCAT
    TATGGATTTT GTGACGTGAT CCCACCTAAT TGTATCCAGC TAAGAAATCT GATCCTGAGT
    GCCTTCCCAA GAAACATGAG GCTCCCAGAC CCATTCACTC CTAATCTGAA GGTGGATATG
    TTGAGTGAAA TTAACATCGC TCCCCGGATT CTCACCAATT TTACTGGAGT GATGCCACCT
    CAATTCAAAA AGGATTTGGA TTCCTATCTT AAAACTCGAT CACCTGTCAC TTTCCTGTCT
    GATCTGCGCA GCAACCTACA GGTATCCAAT GAGCCTGGTA ATCGCTACAA CCTCCAGCTC
    ATTAATGCAC TGGTGCTCTA TGTAGGGACT CAGGCCATTG CACACATCCA CAACAAGGGC
    AGCACACCTT CAATGAGCAC CATCACGCAC TCAGCTCACA TGGACATCTT CCAGAACCTG
    GCTGTGGATT TGGACACTGA AGGTCGGTAT CTCTTCTTGA ATGCAATTGC AAATCAGCTG
    CGGTACCCAA ATAGCCACAC TCACTACTTC AGCTGCACCA TGCTGTACCT TTTTGCAGAG
    GCCAACACTG AAGCTATTCA AGAACAGATC ACAAGGGTTC TTTTGGAACG GTTGATTGTA
    AATAGGCCAC ATCCTTGGGG TCTTCTTATT ACCTTCATTG AGCTGATTAA AAATCCAGCA
    TTTAAATTCT GGAACCATGA ATTTGTACAC TGTGCCCCAG AAATTGAAAA GTTATTCCAG
    TCCGTCGCAC AGTGCTGCAT GGGACAAAAG CAGGCTCAGC AAGTAATGGA AGGGACAGGT
    GCCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.