CD274 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD274
Entrez Gene ID	533834
Full Name	CD274 molecule
Synonyms	PD-L1
General protein information	Preferred Names CD274 molecule Names programmed cell death 1 ligand 1 programmed cell death-ligand 1 programmed death ligand 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010807832.2	XP_010806134.1	programmed cell death 1 ligand 1 isoform X1
XM_010807832.3	XP_010806134.2	programmed cell death 1 ligand 1 isoform X1
XM_024995830.1	XP_024851598.1	programmed cell death 1 ligand 1 isoform X1
XM_024995831.1	XP_024851599.1	programmed cell death 1 ligand 1 isoform X2
XM_005209965.4	XP_005210022.1	programmed cell death 1 ligand 1 isoform X3
XM_024995832.1	XP_024851600.1	programmed cell death 1 ligand 1 isoform X4
XM_005209965.3	XP_005210022.1	programmed cell death 1 ligand 1 isoform X2
NM_001163412.1	NP_001156884.1	programmed cell death 1 ligand 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CD274	NP_054862.1
Canis lupus familiaris (dog)	CD274	XP_005615991.1
Bos taurus (cattle)	CD274	NP_001156884.1
Mus musculus (house mouse)	Cd274	NP_068693.1
Gallus gallus (chicken)	CD274	XP_424811.4
Pan troglodytes (chimpanzee)	CD274	XP_001140705.1
Rattus norvegicus (Norway rat)	Cd274	NP_001178883.1
Danio rerio (zebrafish)	si:ch211-241b2.5	XP_003200855.2
	CD274	NP_001077358.1
Xenopus tropicalis (tropical clawed frog)	cd274	XP_004911464.1

Gene Ontology
Bibliography

Related articles in PubMed

Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-? production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
Goto S, Konnai S, Okagawa T, Nishimori A, Maekawa N, Gondaira S, Higuchi H, Koiwa M, Tajima M, Kohara J, Ogasawara S, Kato Y, Suzuki Y, Murata S, Ohashi K
Immunity, inflammation and disease5(3)355-363(2017 Sep)

Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes.
Ikebuchi R, Konnai S, Okagawa T, Yokoyama K, Nakajima C, Suzuki Y, Murata S, Ohashi K
Immunology142(4)551-61(2014 Aug)

Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade.
Ikebuchi R, Konnai S, Shirai T, Sunden Y, Murata S, Onuma M, Ohashi K
Veterinary research42103(2011 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provides novel information for the understanding of signalling through PD-L1.
Title: Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes.

The following CD274 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD274 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa21950	NM_001163412.1 Latest version!	Bos taurus CD274 molecule (CD274), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa21951	XM_005209965.4 Latest version!	Bos taurus CD274 molecule (CD274), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa52525	XM_010807832.2 Latest version!	Bos taurus CD274 molecule (CD274), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa237162	XM_024995830.1 Latest version!	Bos taurus CD274 molecule (CD274), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa237162	XM_010807832.3 Latest version!	Bos taurus CD274 molecule (CD274), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa237163	XM_024995831.1 Latest version!	Bos taurus CD274 molecule (CD274), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa237164	XM_024995832.1 Latest version!	Bos taurus CD274 molecule (CD274), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OBa21951	XM_005209965.3	Bos taurus CD274 molecule (CD274), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD274 ( NM_001163412.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001156884.1 Bos taurus CD274 molecule (CD274), mRNA.

Price & Availability↑

CloneID	OBa21950
Clone ID Related Accession (Same CDS sequence)	NM_001163412.1
Accession Version	NM_001163412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB510902.1. On Aug 8, 2009 this sequence version replaced XM_613366.4. ##Evidence-Data-START## Transcript exon combination :: AB510902.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGCTA CACAGTTTGA GGAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156884.1
CDS	61..930
Translation	MRIYSVLTFMAYCCLLKAFTITVSKDLYVVEYGSNVTLECRFPVDKQLNLLVLVVYWEMEDKKIIQFVNGKEDPNVQHSSYHGRAQLLKDQLFLGKAALQITDVKLQDAGVYCCLISYGGADYKRITLKVNAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPEPYLDPAKKRNHLVTLGALFLCLSVTLAVIFCLKRDVRMMDVEKCDTRDMNSKQQNATQFEET

Target ORF information:

RefSeq Version	NM_001163412.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163412.1

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGCTA CACAGTTTGA GGAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( XM_005209965.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210022.1 Bos taurus CD274 molecule (CD274), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa21951
Clone ID Related Accession (Same CDS sequence)	XM_005209965.4 , XM_005209965.3
Accession Version	XM_005209965.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209965.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGC TACACAGTTT GAGGAGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210022.1
CDS	191..892
Translation	MRIYSVLTFMAYCCLLKAFTITVSKDLYVVEYGSNVTLECRFPVDKQLNLLVLVVYWEMEDKKIIQFVNGKEDPNVQHSSYHGRAQLLKDQLFLGKAALQITDVKLQDAGVYCCLISYGGADYKRITLKVNAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPATQFEET

Target ORF information:

RefSeq Version	XM_005209965.4
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209965.4

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGC TACACAGTTT GAGGAGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( XM_005209965.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210022.1 Bos taurus CD274 molecule (CD274), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa21951
Clone ID Related Accession (Same CDS sequence)	XM_005209965.4 , XM_005209965.3
Accession Version	XM_005209965.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGC TACACAGTTT GAGGAGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210022.1
CDS	177..878
Translation	MRIYSVLTFMAYCCLLKAFTITVSKDLYVVEYGSNVTLECRFPVDKQLNLLVLVVYWEMEDKKIIQFVNGKEDPNVQHSSYHGRAQLLKDQLFLGKAALQITDVKLQDAGVYCCLISYGGADYKRITLKVNAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPATQFEET

Target ORF information:

RefSeq Version	XM_005209965.3
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209965.3

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGC TACACAGTTT GAGGAGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( XM_010807832.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806134.1 Bos taurus CD274 molecule (CD274), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa52525
Clone ID Related Accession (Same CDS sequence)	XM_010807832.2
Accession Version	XM_010807832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGGTA AGAATTTTAG CAGGGATTGG AAGTTGAAGA AAGGAAACAA AAAGTTAAAG 
AACAAGGGGA AAGCTATCAT TATAATCTCC CCTTATTTTA CTGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806134.1
CDS	176..1126
Translation	MRIYSVLTFMAYCCLLKAFTITVSKDLYVVEYGSNVTLECRFPVDKQLNLLVLVVYWEMEDKKIIQFVNGKEDPNVQHSSYHGRAQLLKDQLFLGKAALQITDVKLQDAGVYCCLISYGGADYKRITLKVNAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPEPYLDPAKKRNHLVTLGALFLCLSVTLAVIFCLKRDVRMMDVEKCDTRDMNSKQQNGKNFSRDWKLKKGNKKLKNKGKAIIIISPYFTEC

Target ORF information:

RefSeq Version	XM_010807832.2
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807832.2

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGGTA AGAATTTTAG CAGGGATTGG AAGTTGAAGA AAGGAAACAA AAAGTTAAAG 
AACAAGGGGA AAGCTATCAT TATAATCTCC CCTTATTTTA CTGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( XM_010807832.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806134.2 Bos taurus CD274 molecule (CD274), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa237162
Clone ID Related Accession (Same CDS sequence)	XM_010807832.3 , XM_024995830.1
Accession Version	XM_010807832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGGGA TTCTCAGCCT GTGGTTTGGG TTTATCAGAC CAGAGGTATG CAGTGGGCCA 
GGGGGCTGCA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806134.2
CDS	194..1108
Translation	MRIYSVLTFMAYCCLLKAFTITVSKDLYVVEYGSNVTLECRFPVDKQLNLLVLVVYWEMEDKKIIQFVNGKEDPNVQHSSYHGRAQLLKDQLFLGKAALQITDVKLQDAGVYCCLISYGGADYKRITLKVNAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPEPYLDPAKKRNHLVTLGALFLCLSVTLAVIFCLKRDVRMMDVEKCDTRDMNSKQQNGILSLWFGFIRPEVCSGPGGCR

Target ORF information:

RefSeq Version	XM_010807832.3
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807832.3

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGGGA TTCTCAGCCT GTGGTTTGGG TTTATCAGAC CAGAGGTATG CAGTGGGCCA 
GGGGGCTGCA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( XM_024995830.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851598.1 Bos taurus CD274 molecule (CD274), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa237162
Clone ID Related Accession (Same CDS sequence)	XM_010807832.3 , XM_024995830.1
Accession Version	XM_024995830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGGGA TTCTCAGCCT GTGGTTTGGG TTTATCAGAC CAGAGGTATG CAGTGGGCCA 
GGGGGCTGCA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851598.1
CDS	183..1097
Translation	MRIYSVLTFMAYCCLLKAFTITVSKDLYVVEYGSNVTLECRFPVDKQLNLLVLVVYWEMEDKKIIQFVNGKEDPNVQHSSYHGRAQLLKDQLFLGKAALQITDVKLQDAGVYCCLISYGGADYKRITLKVNAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPEPYLDPAKKRNHLVTLGALFLCLSVTLAVIFCLKRDVRMMDVEKCDTRDMNSKQQNGILSLWFGFIRPEVCSGPGGCR

Target ORF information:

RefSeq Version	XM_024995830.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995830.1

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGA ACCATATCTA GATCCAGCAA AAAAGAGGAA TCACTTGGTG 
ACTCTGGGAG CTCTCTTCTT ATGCCTGAGT GTAACCCTGG CAGTCATCTT CTGTCTGAAA 
AGAGATGTGA GAATGATGGA TGTAGAAAAA TGTGACACCC GAGATATGAA TTCAAAGCAA 
CAAAATGGGA TTCTCAGCCT GTGGTTTGGG TTTATCAGAC CAGAGGTATG CAGTGGGCCA 
GGGGGCTGCA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( XM_024995831.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851599.1 Bos taurus CD274 molecule (CD274), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa237163
Clone ID Related Accession (Same CDS sequence)	XM_024995831.1
Accession Version	XM_024995831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGG GATTCTCAGC CTGTGGTTTG GGTTTATCAG ACCAGAGGTA 
TGCAGTGGGC CAGGGGGCTG CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851599.1
CDS	196..942
Translation	MRIYSVLTFMAYCCLLKAFTITVSKDLYVVEYGSNVTLECRFPVDKQLNLLVLVVYWEMEDKKIIQFVNGKEDPNVQHSSYHGRAQLLKDQLFLGKAALQITDVKLQDAGVYCCLISYGGADYKRITLKVNAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPGILSLWFGFIRPEVCSGPGGCR

Target ORF information:

RefSeq Version	XM_024995831.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995831.1

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCATTTACT 
ATCACAGTTT CCAAGGACCT GTATGTGGTA GAATATGGCA GCAATGTGAC GTTGGAATGC 
AGATTTCCAG TAGACAAACA ATTAAACCTG CTTGTGTTAG TTGTTTACTG GGAAATGGAG 
GATAAGAAAA TTATTCAGTT TGTGAATGGG AAGGAAGACC CAAACGTTCA GCACAGTAGC 
TACCATGGGA GGGCCCAACT GTTGAAGGAC CAGCTCTTCT TGGGAAAGGC TGCACTTCAG 
ATAACAGATG TGAAATTGCA GGATGCGGGG GTTTACTGTT GCTTGATCAG CTATGGCGGT 
GCCGACTACA AGCGGATTAC TTTGAAAGTC AACGCTCCAT ACCGCAAAAT CTACCATACA 
ATTTCTGTGG ATCCAGTCAC CTCTGAACAT GAACTAACGT GTCAGGCTGA GGGTTACCCT 
GAAGCTGATG TCATCTGGAC AAGTAGCGAT CACCAAGTCC TGAGTGGCAA AACCAGCATC 
ACCAGTTCAA AGAGGGAGGA AAAGCTTTTC AATGTGACCA GCACACTGAG AATCAACACA 
ACAGCTGACA AAATTTTCTA CTGCACTTTT CGGAGATTAG GTCATGAGGA AAACAACACA 
GCTGAGTTGG TCATCCCAGG GATTCTCAGC CTGTGGTTTG GGTTTATCAG ACCAGAGGTA 
TGCAGTGGGC CAGGGGGCTG CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD274 ( XM_024995832.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851600.1 Bos taurus CD274 molecule (CD274), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa237164
Clone ID Related Accession (Same CDS sequence)	XM_024995832.1
Accession Version	XM_024995832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	programmed cell death 1 ligand 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCTCCATAC 
CGCAAAATCT ACCATACAAT TTCTGTGGAT CCAGTCACCT CTGAACATGA ACTAACGTGT 
CAGGCTGAGG GTTACCCTGA AGCTGATGTC ATCTGGACAA GTAGCGATCA CCAAGTCCTG 
AGTGGCAAAA CCAGCATCAC CAGTTCAAAG AGGGAGGAAA AGCTTTTCAA TGTGACCAGC 
ACACTGAGAA TCAACACAAC AGCTGACAAA ATTTTCTACT GCACTTTTCG GAGATTAGGT 
CATGAGGAAA ACAACACAGC TGAGTTGGTC ATCCCAGAAC CATATCTAGA TCCAGCAAAA 
AAGAGGAATC ACTTGGTGAC TCTGGGAGCT CTCTTCTTAT GCCTGAGTGT AACCCTGGCA 
GTCATCTTCT GTCTGAAAAG AGATGTGAGA ATGATGGATG TAGAAAAATG TGACACCCGA 
GATATGAATT CAAAGCAACA AAATGGGATT CTCAGCCTGT GGTTTGGGTT TATCAGACCA 
GAGGTATGCA GTGGGCCAGG GGGCTGCAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851600.1
CDS	239..811
Translation	MRIYSVLTFMAYCCLLKAPYRKIYHTISVDPVTSEHELTCQAEGYPEADVIWTSSDHQVLSGKTSITSSKREEKLFNVTSTLRINTTADKIFYCTFRRLGHEENNTAELVIPEPYLDPAKKRNHLVTLGALFLCLSVTLAVIFCLKRDVRMMDVEKCDTRDMNSKQQNGILSLWFGFIRPEVCSGPGGCR

Target ORF information:

RefSeq Version	XM_024995832.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD274 molecule (CD274), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995832.1

ORF Insert Sequence:

ATGAGGATAT ATAGTGTCTT AACATTCATG GCTTACTGTT GTTTGCTGAA AGCTCCATAC 
CGCAAAATCT ACCATACAAT TTCTGTGGAT CCAGTCACCT CTGAACATGA ACTAACGTGT 
CAGGCTGAGG GTTACCCTGA AGCTGATGTC ATCTGGACAA GTAGCGATCA CCAAGTCCTG 
AGTGGCAAAA CCAGCATCAC CAGTTCAAAG AGGGAGGAAA AGCTTTTCAA TGTGACCAGC 
ACACTGAGAA TCAACACAAC AGCTGACAAA ATTTTCTACT GCACTTTTCG GAGATTAGGT 
CATGAGGAAA ACAACACAGC TGAGTTGGTC ATCCCAGAAC CATATCTAGA TCCAGCAAAA 
AAGAGGAATC ACTTGGTGAC TCTGGGAGCT CTCTTCTTAT GCCTGAGTGT AACCCTGGCA 
GTCATCTTCT GTCTGAAAAG AGATGTGAGA ATGATGGATG TAGAAAAATG TGACACCCGA 
GATATGAATT CAAAGCAACA AAATGGGATT CTCAGCCTGT GGTTTGGGTT TATCAGACCA 
GAGGTATGCA GTGGGCCAGG GGGCTGCAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-? production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
Immunity, inflammation and disease5(3)355-363(2017 Sep)
Goto S,Konnai S,Okagawa T,Nishimori A,Maekawa N,Gondaira S,Higuchi H,Koiwa M,Tajima M,Kohara J,Ogasawara S,Kato Y,Suzuki Y,Murata S,Ohashi K

Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes.
Immunology142(4)551-61(2014 Aug)
Ikebuchi R,Konnai S,Okagawa T,Yokoyama K,Nakajima C,Suzuki Y,Murata S,Ohashi K

Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade.
Veterinary research42103(2011 Sep)
Ikebuchi R,Konnai S,Shirai T,Sunden Y,Murata S,Onuma M,Ohashi K

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CD274 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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