PRAG1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015460864.1	XP_015316350.1	tyrosine-protein kinase SgK223 isoform X2
XM_005226036.3	XP_005226093.1	tyrosine-protein kinase SgK223 isoform X1
XM_010826099.2	XP_010824401.2	tyrosine-protein kinase SgK223 isoform X1
XM_010820297.2	XP_010818599.1	tyrosine-protein kinase SgK223 isoform X1
XM_005226034.3	XP_005226091.1	tyrosine-protein kinase SgK223 isoform X1
XM_015469735.1	XP_015325221.1	tyrosine-protein kinase SgK223 isoform X1
XM_015469736.1	XP_015325222.1	tyrosine-protein kinase SgK223 isoform X1
XM_015469737.1	XP_015325223.1	tyrosine-protein kinase SgK223 isoform X2
XM_005226034.4	XP_005226091.1	tyrosine-protein kinase PRAG1 isoform X1
XM_015460864.2	XP_015316350.1	tyrosine-protein kinase PRAG1 isoform X2
XM_024986466.1	XP_024842234.1	tyrosine-protein kinase PRAG1 isoform X3
XM_005226036.4	XP_005226093.1	tyrosine-protein kinase PRAG1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa33957	XM_015469735.1 Latest version!	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa33957	XM_015469736.1 Latest version!	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa33957	XM_005226034.4 Latest version!	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa33957	XM_005226036.4 Latest version!	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa33957	XM_010826099.2 Latest version!	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa33957	XM_010820297.2 Latest version!	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa231499	XM_024986466.1 Latest version!	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa88845	XM_015469737.1 Latest version!	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa88845	XM_015460864.2 Latest version!	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OBa33957	XM_005226034.3	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OBa88845	XM_015460864.1	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa33957	XM_005226036.3	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
Hide Old Accession Versions >>

PRAG1 ( XM_005226036.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226093.1 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_005226036.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226093.1
CDS	363..4589
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005226036.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226036.3

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_010826099.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824401.2 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_010826099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646720.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824401.2
CDS	227..4453
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_010826099.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826099.2

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_010820297.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818599.1 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X3, mRNA.

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CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_010820297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818599.1
CDS	667..4893
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_010820297.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820297.2

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_005226034.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226091.1 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X1, mRNA.

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CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_005226034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226091.1
CDS	227..4453
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005226034.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226034.3

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_015469735.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325221.1 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_015469735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646720.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325221.1
CDS	363..4589
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_015469735.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469735.1

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_015469736.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325222.1 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_015469736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646720.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325222.1
CDS	667..4893
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_015469736.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469736.1

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_005226034.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226091.1 Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X1, mRNA.

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CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_005226034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase PRAG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226091.1
CDS	241..4467
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005226034.4
Organism	Bos taurus(cattle)
Definition	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226034.4

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_005226036.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226093.1 Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X2, mRNA.

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CloneID	OBa33957
Clone ID Related Accession (Same CDS sequence)	XM_005226036.3 , XM_010826099.2 , XM_010820297.2 , XM_005226034.3 , XM_015469735.1 , XM_015469736.1 , XM_005226034.4 , XM_005226036.4
Accession Version	XM_005226036.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase PRAG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226036.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226093.1
CDS	354..4580
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQVIWRRAPGKLPLPKQEDGPVVYLGSFRGVQKPAGPSGPTDGGHPRSPPAYAMVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_005226036.4
Organism	Bos taurus(cattle)
Definition	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226036.4

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GTCATCTGGA GACGAGCCCC CGGCAAGCTC 
CCCCTCCCAA AGCAGGAAGA CGGGCCAGTA GTTTATCTGG GCAGCTTTCG AGGCGTCCAG 
AAGCCTGCCG GGCCCTCGGG TCCCACAGAC GGGGGTCACC CCCGCAGCCC CCCTGCCTAT 
GCCATGGTCG GCCTGCATAG CCTAGAGCCC CGGGGGGACC GGAGTGCCGC CTTCCACCCG 
GTGAGCTTCC CCGACGAGAA GCTTGGACGA GACGATAAAC CAGTGATCCC CTACCAAGAG 
CTAAACTCCC CGCAGGAGAG CTTCCGGCAG AAGCTGGCCG CCTTCTCCGG GATGACTTCC 
GGCTGCCTCA AGGGCCTGGG GCCCTACCCG TCTGCTCAGC CCATGCGGGA GTCCCTGCCC 
TCGGAGGATG ACAGCGATCA AAGGTGCTCA CCCTCGGGGG ACAGCGAAGG GGGAGAATAC 
TGCTCCATCC TGGACTGCTG CCCCGGGAGC CCAGGCGCGG AGGACGCCGC CCGGGCCGGC 
GGTTCCCGAC ACAGACAGGG CGGCAGGGAC TGCTCGGCAG CCTGCTGGGA GCCGCGGGTG 
TGTGCCAGGC CACCTGAGGA GGAGAAGCAG GCTTTGAGTT TTCCCAGGGA CTGCTGCAGC 
CAGGGCCCGA CCGAGAGCCC GCCCCGCCTG GGCCCCAAAA AGCAGTCCCT CGCTTCAGAG 
GCTGCCAGCT CTTCGGATGG CCTCTCCTGT GGGAGCGCCA ACAGCCGCGC CAGCAGTCCC 
CTGGCCCCCC ATCCTGAGAG CGATTACTGC TCCCTCAGGA AGGAGCCTGG GCTGGGGAAG 
CAGCAGGACT CCGGCTGCCA CGGCGTGGCC TCCAGCAGGC GCCTTGGGCA GACTGGGGAG 
ACCCAGCCCC CCGCCCACTC CAGGGAGGCT GCTCAGTCTG AACCCATCTA TGCTGAGAGT 
ACCAAGAGGA AGAAGGCCGT TCCAGTGCCT TCCAGGCCAC AGGCCAAGGC AGAACAGGGG 
GCAGGCGCTC CGGGCCAGGC CCAGGTGCGG ACAGTTAACA CCTGGGCTCA GAAAGCCGCA 
TCTGGCTGGG GCCAGGTCAG GGAAGGCCCG GATGCGGCCC CCCAGATGGC GGCCACCATC 
ACAGTCATGG CCGCCCACCC AGAAGAGGAC CACCGGACAA TCTACCTGAG CAGCCCAGAC 
TCCTCTGTGG GGGTGCAGTG GCCACGCGGG CCCCCGAGCC AGCAGGCTCC TGAGGCAGGC 
GGAGAGGAGC CTTCAGCTGG GCAGGGGCTC GGCTCCAGGG AGAGCCGGCA TCGCAGTGCC 
AGCGAGACCA CGGCCAAGGG GAGACCTGCC ATCCCCCCCA AGATGTCGAG GAGCAGCCCT 
GGAGGGTCCC CGGTATCCCC CTCCGCCTAC CCACTGTCCG ACCTCAGTGA AGGGAACTCA 
GGCACCCCTG GGCCCCAGCC TCCATCCAAG GGCCCCGCTG ACCCCGCTTC TTCCTGCCGG 
GCCAATGGCA TCCCCTCCAG TGACTCTGCC AGGGGCCCCC CGCCTGCCAC CAGTACGTCA 
GCCTTGGAGC AGAGGCGGCC CCGGCCCCAG GCCGGTGCCT GGAGTCGCCA GTGCCGGATT 
GAGGAGGAGG ATGAGGTGGA GCAGGACTTG CTGAGTCAAA GTTGGGGGAG AGAGGTGGAA 
AATGGCCCCT TGGACCCGGC CAGCTCCTCC GCCTGGCACC GTCTTCGCCC CACAGATGGC 
TCCTCTGGGC AGAACAGCAA AGCTGGGACT GGGATGAGCA AGTCGGCCTC ATTTGCCTTT 
GAGTTCCCCA AGGACAGAAG CGGGATTGAG ACGTTCTCTC CTCCTCCGCC ACCTCCAAAG 
TCACGGCACC TTTTAAAAAT GAACAAGAGC AGCTCTGATT TGGAGAAGGT GAGCCAGGGC 
TCTGCCGAGA GCCTCAGCCC GTCCTTCAGG GGCGTCCACG TCAGCTTCAC CACGGGCTCC 
ACGGACAGCC TGGCCTCTGA CTCCAGGACC TGCAGCGATG GAGGTCCATC ATCTGAGCCA 
GCTCACTCGC CCACCAACAG CAGGAAGAAG CTCTTTGCCC CCGTCCCCTT CCCTTCAGGC 
TCCACAGAGG ATGTGTCCCC CGGCGACCCC CTGCAGCCCC CGCCTCTTCC CCAGAAGAAG 
CTCGTGAGTC GGGCGGCCTC TTCCCCTGAT GGCTTCTTCT GGACCCAGGG CTCCCCCAAA 
CCCAGAACAG CAAGTCCCAA GCTGAACCTC AGCCACTCGG AGACTAACGT CTGTGCCCAT 
GAGGACTCCC ACTTCAGCTA CGCCTCAGGC CCTGGCGGCC GCCACCAGCA CGTCTTCTCC 
GCTTCCGAGC CGCTGGAGAA AACTTCCAAA GGCAGTGGCC ACCGCGGCCC TGTGCTGGGG 
CTGGGGGGCA GCAAAGGCCT GCAGGGCAAA GGGGTCTCCT CCGCCTCGTC CTCTCAGCTG 
AGCGTGTCCA GCCAGGCCTC GGCGGGCAGC ACTCAGCTGC AGCTCCACAG CCTCCTGAAC 
AGCATCAGCA GCAAAGAGGG CACCTACGCC AAGCTGGGGG GGCTCTACGC CCAGTCCCTG 
GTCCGCCTCG TGGCCAGGTG CGAGGACCTC TTCATGGGTG GCCAGAAGAA GGAGCTGCAC 
TTCAGCGAGA ATAACTGGTC CCTGTTCAAG CTGACCTGTA ACAAGCCCTG CTGTGACTCG 
GGGGATGCCA TTTACTATTG CGCCACCTGC TCCGAGGACC CCGGCAGCAC CTACGCCGTG 
AAGATCTGCA AGACCCCCGA GCCCAAAGCA GCCTCCTACT GCAGCCCTTC AGTGCCCGTG 
CACTTCAATA TCCAGCAGGA CTGTGGCCAC TTTGTCGCCT CCGTGCCCTC CAGCATGCTC 
ACCTCTCCCG ACGCACACAA GGACCCTCTG TCTGCCCTGC CCTCACATCC CCCCGCCCAG 
GAGCAGGACT GCGTGGTGGT CATCACTCGG GAGGTGCCCC ACCAAACCGC CTCGGACTTT 
GTGCGGGACT TGGCCGCCAG CCACCAGTCT GAGCCGGAGG TTTACGAGCG GCGGGTGTGC 
TTCCTGCTCC TGCAGCTCTG CAACGGGCTG GAGCACCTGA AGGAGCATGG GATCATCCAC 
CGAGACCTGT GCCTGGAGAA CCTGCTGCTG GTGCATTGCG CCCCCCAGGC CTCCCCCGAG 
CCTTCCACCG CTGCCTCCCT CCCTCCCACC ACCACATCCC CTGCCCCTGC GGCTACCCCC 
TCCTCTGGCC CCTCAGCCAC CGTCCCTACC AGCGTCAACC CCACCCCTCC AGCCGCCCCC 
GCCTTTCAAG GAGGGTCCAG TGAGAAACAC TTGCCCCGGC TTATCATCAG CAACTTCTTG 
AAGGCCAAGC AGAAGCCAGG TGGTACCACC AACCTGCAGC AGAAGAAGAG CCAGGCACGC 
CTGGCCCCCG AGATCGTGTC GGCCTCCCAG TACCGCAAGT TCGACGAATT CCAAACGGGC 
ATCCTCATCT ACGAGCTGCT CCACCAGCCC AACCCATTCG AGGTGCGGGC GCAGCTCCGG 
GAGCAGGACT ACCGGCAGGA GGACTTGCCC CCGCTGCCCG CGCTGTCCCT CTACTCGCCG 
GGTCTGCAGC GGCTCGCCCA CCTGCTGCTG GAGGCCGACC CCATCAAGCG CATCCGCATC 
GGCGAGGCCA AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CCCGGCGCGA GCTGGTGGAG 
CCGCCGGGCG CCTCGGAGGA GGTGCTGTGC ACCACGCTCC ACAACTGGAT CGACATGAAG 
CGAGCCCTGA TGATGATGAA GTTCGCCGAG AAGGCGGTGG ACCGCCGGCG CGGGGTGGAG 
CTGGAGGACT GGCTCTGCTG CCAGTATCTG GCGTCGGCCG AGCCCACAGC CCTCTTACAA 
TCACTGAAGC TCCTACAGCT TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_024986466.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842234.1 Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X4, mRNA.

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CloneID	OBa231499
Clone ID Related Accession (Same CDS sequence)	XM_024986466.1
Accession Version	XM_024986466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase PRAG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGGCC TGCATAGCCT AGAGCCCCGG GGGGACCGGA GTGCCGCCTT CCACCCGGTG 
AGCTTCCCCG ACGAGAAGCT TGGACGAGAC GATAAACCAG TGATCCCCTA CCAAGAGCTA 
AACTCCCCGC AGGAGAGCTT CCGGCAGAAG CTGGCCGCCT TCTCCGGGAT GACTTCCGGC 
TGCCTCAAGG GCCTGGGGCC CTACCCGTCT GCTCAGCCCA TGCGGGAGTC CCTGCCCTCG 
GAGGATGACA GCGATCAAAG GTGCTCACCC TCGGGGGACA GCGAAGGGGG AGAATACTGC 
TCCATCCTGG ACTGCTGCCC CGGGAGCCCA GGCGCGGAGG ACGCCGCCCG GGCCGGCGGT 
TCCCGACACA GACAGGGCGG CAGGGACTGC TCGGCAGCCT GCTGGGAGCC GCGGGTGTGT 
GCCAGGCCAC CTGAGGAGGA GAAGCAGGCT TTGAGTTTTC CCAGGGACTG CTGCAGCCAG 
GGCCCGACCG AGAGCCCGCC CCGCCTGGGC CCCAAAAAGC AGTCCCTCGC TTCAGAGGCT 
GCCAGCTCTT CGGATGGCCT CTCCTGTGGG AGCGCCAACA GCCGCGCCAG CAGTCCCCTG 
GCCCCCCATC CTGAGAGCGA TTACTGCTCC CTCAGGAAGG AGCCTGGGCT GGGGAAGCAG 
CAGGACTCCG GCTGCCACGG CGTGGCCTCC AGCAGGCGCC TTGGGCAGAC TGGGGAGACC 
CAGCCCCCCG CCCACTCCAG GGAGGCTGCT CAGTCTGAAC CCATCTATGC TGAGAGTACC 
AAGAGGAAGA AGGCCGTTCC AGTGCCTTCC AGGCCACAGG CCAAGGCAGA ACAGGGGGCA 
GGCGCTCCGG GCCAGGCCCA GGTGCGGACA GTTAACACCT GGGCTCAGAA AGCCGCATCT 
GGCTGGGGCC AGGTCAGGGA AGGCCCGGAT GCGGCCCCCC AGATGGCGGC CACCATCACA 
GTCATGGCCG CCCACCCAGA AGAGGACCAC CGGACAATCT ACCTGAGCAG CCCAGACTCC 
TCTGTGGGGG TGCAGTGGCC ACGCGGGCCC CCGAGCCAGC AGGCTCCTGA GGCAGGCGGA 
GAGGAGCCTT CAGCTGGGCA GGGGCTCGGC TCCAGGGAGA GCCGGCATCG CAGTGCCAGC 
GAGACCACGG CCAAGGGGAG ACCTGCCATC CCCCCCAAGA TGTCGAGGAG CAGCCCTGGA 
GGGTCCCCGG TATCCCCCTC CGCCTACCCA CTGTCCGACC TCAGTGAAGG GAACTCAGGC 
ACCCCTGGGC CCCAGCCTCC ATCCAAGGGC CCCGCTGACC CCGCTTCTTC CTGCCGGGCC 
AATGGCATCC CCTCCAGTGA CTCTGCCAGG GGCCCCCCGC CTGCCACCAG TACGTCAGCC 
TTGGAGCAGA GGCGGCCCCG GCCCCAGGCC GGTGCCTGGA GTCGCCAGTG CCGGATTGAG 
GAGGAGGATG AGGTGGAGCA GGACTTGCTG AGTCAAAGTT GGGGGAGAGA GGTGGAAAAT 
GGCCCCTTGG ACCCGGCCAG CTCCTCCGCC TGGCACCGTC TTCGCCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGC TGGGACTGGG ATGAGCAAGT CGGCCTCATT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGCGG GATTGAGACG TTCTCTCCTC CTCCGCCACC TCCAAAGTCA 
CGGCACCTTT TAAAAATGAA CAAGAGCAGC TCTGATTTGG AGAAGGTGAG CCAGGGCTCT 
GCCGAGAGCC TCAGCCCGTC CTTCAGGGGC GTCCACGTCA GCTTCACCAC GGGCTCCACG 
GACAGCCTGG CCTCTGACTC CAGGACCTGC AGCGATGGAG GTCCATCATC TGAGCCAGCT 
CACTCGCCCA CCAACAGCAG GAAGAAGCTC TTTGCCCCCG TCCCCTTCCC TTCAGGCTCC 
ACAGAGGATG TGTCCCCCGG CGACCCCCTG CAGCCCCCGC CTCTTCCCCA GAAGAAGCTC 
GTGAGTCGGG CGGCCTCTTC CCCTGATGGC TTCTTCTGGA CCCAGGGCTC CCCCAAACCC 
AGAACAGCAA GTCCCAAGCT GAACCTCAGC CACTCGGAGA CTAACGTCTG TGCCCATGAG 
GACTCCCACT TCAGCTACGC CTCAGGCCCT GGCGGCCGCC ACCAGCACGT CTTCTCCGCT 
TCCGAGCCGC TGGAGAAAAC TTCCAAAGGC AGTGGCCACC GCGGCCCTGT GCTGGGGCTG 
GGGGGCAGCA AAGGCCTGCA GGGCAAAGGG GTCTCCTCCG CCTCGTCCTC TCAGCTGAGC 
GTGTCCAGCC AGGCCTCGGC GGGCAGCACT CAGCTGCAGC TCCACAGCCT CCTGAACAGC 
ATCAGCAGCA AAGAGGGCAC CTACGCCAAG CTGGGGGGGC TCTACGCCCA GTCCCTGGTC 
CGCCTCGTGG CCAGGTGCGA GGACCTCTTC ATGGGTGGCC AGAAGAAGGA GCTGCACTTC 
AGCGAGAATA ACTGGTCCCT GTTCAAGCTG ACCTGTAACA AGCCCTGCTG TGACTCGGGG 
GATGCCATTT ACTATTGCGC CACCTGCTCC GAGGACCCCG GCAGCACCTA CGCCGTGAAG 
ATCTGCAAGA CCCCCGAGCC CAAAGCAGCC TCCTACTGCA GCCCTTCAGT GCCCGTGCAC 
TTCAATATCC AGCAGGACTG TGGCCACTTT GTCGCCTCCG TGCCCTCCAG CATGCTCACC 
TCTCCCGACG CACACAAGGA CCCTCTGTCT GCCCTGCCCT CACATCCCCC CGCCCAGGAG 
CAGGACTGCG TGGTGGTCAT CACTCGGGAG GTGCCCCACC AAACCGCCTC GGACTTTGTG 
CGGGACTTGG CCGCCAGCCA CCAGTCTGAG CCGGAGGTTT ACGAGCGGCG GGTGTGCTTC 
CTGCTCCTGC AGCTCTGCAA CGGGCTGGAG CACCTGAAGG AGCATGGGAT CATCCACCGA 
GACCTGTGCC TGGAGAACCT GCTGCTGGTG CATTGCGCCC CCCAGGCCTC CCCCGAGCCT 
TCCACCGCTG CCTCCCTCCC TCCCACCACC ACATCCCCTG CCCCTGCGGC TACCCCCTCC 
TCTGGCCCCT CAGCCACCGT CCCTACCAGC GTCAACCCCA CCCCTCCAGC CGCCCCCGCC 
TTTCAAGGAG GGTCCAGTGA GAAACACTTG CCCCGGCTTA TCATCAGCAA CTTCTTGAAG 
GCCAAGCAGA AGCCAGGTGG TACCACCAAC CTGCAGCAGA AGAAGAGCCA GGCACGCCTG 
GCCCCCGAGA TCGTGTCGGC CTCCCAGTAC CGCAAGTTCG ACGAATTCCA AACGGGCATC 
CTCATCTACG AGCTGCTCCA CCAGCCCAAC CCATTCGAGG TGCGGGCGCA GCTCCGGGAG 
CAGGACTACC GGCAGGAGGA CTTGCCCCCG CTGCCCGCGC TGTCCCTCTA CTCGCCGGGT 
CTGCAGCGGC TCGCCCACCT GCTGCTGGAG GCCGACCCCA TCAAGCGCAT CCGCATCGGC 
GAGGCCAAGC GCGTGCTGCA GTGCCTGCTG TGGGGGCCCC GGCGCGAGCT GGTGGAGCCG 
CCGGGCGCCT CGGAGGAGGT GCTGTGCACC ACGCTCCACA ACTGGATCGA CATGAAGCGA 
GCCCTGATGA TGATGAAGTT CGCCGAGAAG GCGGTGGACC GCCGGCGCGG GGTGGAGCTG 
GAGGACTGGC TCTGCTGCCA GTATCTGGCG TCGGCCGAGC CCACAGCCCT CTTACAATCA 
CTGAAGCTCC TACAGCTTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842234.1
CDS	744..4487
Translation	MVGLHSLEPRGDRSAAFHPVSFPDEKLGRDDKPVIPYQELNSPQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_024986466.1
Organism	Bos taurus(cattle)
Definition	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986466.1

ORF Insert Sequence:

ATGGTCGGCC TGCATAGCCT AGAGCCCCGG GGGGACCGGA GTGCCGCCTT CCACCCGGTG 
AGCTTCCCCG ACGAGAAGCT TGGACGAGAC GATAAACCAG TGATCCCCTA CCAAGAGCTA 
AACTCCCCGC AGGAGAGCTT CCGGCAGAAG CTGGCCGCCT TCTCCGGGAT GACTTCCGGC 
TGCCTCAAGG GCCTGGGGCC CTACCCGTCT GCTCAGCCCA TGCGGGAGTC CCTGCCCTCG 
GAGGATGACA GCGATCAAAG GTGCTCACCC TCGGGGGACA GCGAAGGGGG AGAATACTGC 
TCCATCCTGG ACTGCTGCCC CGGGAGCCCA GGCGCGGAGG ACGCCGCCCG GGCCGGCGGT 
TCCCGACACA GACAGGGCGG CAGGGACTGC TCGGCAGCCT GCTGGGAGCC GCGGGTGTGT 
GCCAGGCCAC CTGAGGAGGA GAAGCAGGCT TTGAGTTTTC CCAGGGACTG CTGCAGCCAG 
GGCCCGACCG AGAGCCCGCC CCGCCTGGGC CCCAAAAAGC AGTCCCTCGC TTCAGAGGCT 
GCCAGCTCTT CGGATGGCCT CTCCTGTGGG AGCGCCAACA GCCGCGCCAG CAGTCCCCTG 
GCCCCCCATC CTGAGAGCGA TTACTGCTCC CTCAGGAAGG AGCCTGGGCT GGGGAAGCAG 
CAGGACTCCG GCTGCCACGG CGTGGCCTCC AGCAGGCGCC TTGGGCAGAC TGGGGAGACC 
CAGCCCCCCG CCCACTCCAG GGAGGCTGCT CAGTCTGAAC CCATCTATGC TGAGAGTACC 
AAGAGGAAGA AGGCCGTTCC AGTGCCTTCC AGGCCACAGG CCAAGGCAGA ACAGGGGGCA 
GGCGCTCCGG GCCAGGCCCA GGTGCGGACA GTTAACACCT GGGCTCAGAA AGCCGCATCT 
GGCTGGGGCC AGGTCAGGGA AGGCCCGGAT GCGGCCCCCC AGATGGCGGC CACCATCACA 
GTCATGGCCG CCCACCCAGA AGAGGACCAC CGGACAATCT ACCTGAGCAG CCCAGACTCC 
TCTGTGGGGG TGCAGTGGCC ACGCGGGCCC CCGAGCCAGC AGGCTCCTGA GGCAGGCGGA 
GAGGAGCCTT CAGCTGGGCA GGGGCTCGGC TCCAGGGAGA GCCGGCATCG CAGTGCCAGC 
GAGACCACGG CCAAGGGGAG ACCTGCCATC CCCCCCAAGA TGTCGAGGAG CAGCCCTGGA 
GGGTCCCCGG TATCCCCCTC CGCCTACCCA CTGTCCGACC TCAGTGAAGG GAACTCAGGC 
ACCCCTGGGC CCCAGCCTCC ATCCAAGGGC CCCGCTGACC CCGCTTCTTC CTGCCGGGCC 
AATGGCATCC CCTCCAGTGA CTCTGCCAGG GGCCCCCCGC CTGCCACCAG TACGTCAGCC 
TTGGAGCAGA GGCGGCCCCG GCCCCAGGCC GGTGCCTGGA GTCGCCAGTG CCGGATTGAG 
GAGGAGGATG AGGTGGAGCA GGACTTGCTG AGTCAAAGTT GGGGGAGAGA GGTGGAAAAT 
GGCCCCTTGG ACCCGGCCAG CTCCTCCGCC TGGCACCGTC TTCGCCCCAC AGATGGCTCC 
TCTGGGCAGA ACAGCAAAGC TGGGACTGGG ATGAGCAAGT CGGCCTCATT TGCCTTTGAG 
TTCCCCAAGG ACAGAAGCGG GATTGAGACG TTCTCTCCTC CTCCGCCACC TCCAAAGTCA 
CGGCACCTTT TAAAAATGAA CAAGAGCAGC TCTGATTTGG AGAAGGTGAG CCAGGGCTCT 
GCCGAGAGCC TCAGCCCGTC CTTCAGGGGC GTCCACGTCA GCTTCACCAC GGGCTCCACG 
GACAGCCTGG CCTCTGACTC CAGGACCTGC AGCGATGGAG GTCCATCATC TGAGCCAGCT 
CACTCGCCCA CCAACAGCAG GAAGAAGCTC TTTGCCCCCG TCCCCTTCCC TTCAGGCTCC 
ACAGAGGATG TGTCCCCCGG CGACCCCCTG CAGCCCCCGC CTCTTCCCCA GAAGAAGCTC 
GTGAGTCGGG CGGCCTCTTC CCCTGATGGC TTCTTCTGGA CCCAGGGCTC CCCCAAACCC 
AGAACAGCAA GTCCCAAGCT GAACCTCAGC CACTCGGAGA CTAACGTCTG TGCCCATGAG 
GACTCCCACT TCAGCTACGC CTCAGGCCCT GGCGGCCGCC ACCAGCACGT CTTCTCCGCT 
TCCGAGCCGC TGGAGAAAAC TTCCAAAGGC AGTGGCCACC GCGGCCCTGT GCTGGGGCTG 
GGGGGCAGCA AAGGCCTGCA GGGCAAAGGG GTCTCCTCCG CCTCGTCCTC TCAGCTGAGC 
GTGTCCAGCC AGGCCTCGGC GGGCAGCACT CAGCTGCAGC TCCACAGCCT CCTGAACAGC 
ATCAGCAGCA AAGAGGGCAC CTACGCCAAG CTGGGGGGGC TCTACGCCCA GTCCCTGGTC 
CGCCTCGTGG CCAGGTGCGA GGACCTCTTC ATGGGTGGCC AGAAGAAGGA GCTGCACTTC 
AGCGAGAATA ACTGGTCCCT GTTCAAGCTG ACCTGTAACA AGCCCTGCTG TGACTCGGGG 
GATGCCATTT ACTATTGCGC CACCTGCTCC GAGGACCCCG GCAGCACCTA CGCCGTGAAG 
ATCTGCAAGA CCCCCGAGCC CAAAGCAGCC TCCTACTGCA GCCCTTCAGT GCCCGTGCAC 
TTCAATATCC AGCAGGACTG TGGCCACTTT GTCGCCTCCG TGCCCTCCAG CATGCTCACC 
TCTCCCGACG CACACAAGGA CCCTCTGTCT GCCCTGCCCT CACATCCCCC CGCCCAGGAG 
CAGGACTGCG TGGTGGTCAT CACTCGGGAG GTGCCCCACC AAACCGCCTC GGACTTTGTG 
CGGGACTTGG CCGCCAGCCA CCAGTCTGAG CCGGAGGTTT ACGAGCGGCG GGTGTGCTTC 
CTGCTCCTGC AGCTCTGCAA CGGGCTGGAG CACCTGAAGG AGCATGGGAT CATCCACCGA 
GACCTGTGCC TGGAGAACCT GCTGCTGGTG CATTGCGCCC CCCAGGCCTC CCCCGAGCCT 
TCCACCGCTG CCTCCCTCCC TCCCACCACC ACATCCCCTG CCCCTGCGGC TACCCCCTCC 
TCTGGCCCCT CAGCCACCGT CCCTACCAGC GTCAACCCCA CCCCTCCAGC CGCCCCCGCC 
TTTCAAGGAG GGTCCAGTGA GAAACACTTG CCCCGGCTTA TCATCAGCAA CTTCTTGAAG 
GCCAAGCAGA AGCCAGGTGG TACCACCAAC CTGCAGCAGA AGAAGAGCCA GGCACGCCTG 
GCCCCCGAGA TCGTGTCGGC CTCCCAGTAC CGCAAGTTCG ACGAATTCCA AACGGGCATC 
CTCATCTACG AGCTGCTCCA CCAGCCCAAC CCATTCGAGG TGCGGGCGCA GCTCCGGGAG 
CAGGACTACC GGCAGGAGGA CTTGCCCCCG CTGCCCGCGC TGTCCCTCTA CTCGCCGGGT 
CTGCAGCGGC TCGCCCACCT GCTGCTGGAG GCCGACCCCA TCAAGCGCAT CCGCATCGGC 
GAGGCCAAGC GCGTGCTGCA GTGCCTGCTG TGGGGGCCCC GGCGCGAGCT GGTGGAGCCG 
CCGGGCGCCT CGGAGGAGGT GCTGTGCACC ACGCTCCACA ACTGGATCGA CATGAAGCGA 
GCCCTGATGA TGATGAAGTT CGCCGAGAAG GCGGTGGACC GCCGGCGCGG GGTGGAGCTG 
GAGGACTGGC TCTGCTGCCA GTATCTGGCG TCGGCCGAGC CCACAGCCCT CTTACAATCA 
CTGAAGCTCC TACAGCTTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_015460864.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316350.1 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa88845
Clone ID Related Accession (Same CDS sequence)	XM_015460864.1 , XM_015469737.1 , XM_015460864.2
Accession Version	XM_015460864.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GAGAGCTTCC GGCAGAAGCT GGCCGCCTTC 
TCCGGGATGA CTTCCGGCTG CCTCAAGGGC CTGGGGCCCT ACCCGTCTGC TCAGCCCATG 
CGGGAGTCCC TGCCCTCGGA GGATGACAGC GATCAAAGGT GCTCACCCTC GGGGGACAGC 
GAAGGGGGAG AATACTGCTC CATCCTGGAC TGCTGCCCCG GGAGCCCAGG CGCGGAGGAC 
GCCGCCCGGG CCGGCGGTTC CCGACACAGA CAGGGCGGCA GGGACTGCTC GGCAGCCTGC 
TGGGAGCCGC GGGTGTGTGC CAGGCCACCT GAGGAGGAGA AGCAGGCTTT GAGTTTTCCC 
AGGGACTGCT GCAGCCAGGG CCCGACCGAG AGCCCGCCCC GCCTGGGCCC CAAAAAGCAG 
TCCCTCGCTT CAGAGGCTGC CAGCTCTTCG GATGGCCTCT CCTGTGGGAG CGCCAACAGC 
CGCGCCAGCA GTCCCCTGGC CCCCCATCCT GAGAGCGATT ACTGCTCCCT CAGGAAGGAG 
CCTGGGCTGG GGAAGCAGCA GGACTCCGGC TGCCACGGCG TGGCCTCCAG CAGGCGCCTT 
GGGCAGACTG GGGAGACCCA GCCCCCCGCC CACTCCAGGG AGGCTGCTCA GTCTGAACCC 
ATCTATGCTG AGAGTACCAA GAGGAAGAAG GCCGTTCCAG TGCCTTCCAG GCCACAGGCC 
AAGGCAGAAC AGGGGGCAGG CGCTCCGGGC CAGGCCCAGG TGCGGACAGT TAACACCTGG 
GCTCAGAAAG CCGCATCTGG CTGGGGCCAG GTCAGGGAAG GCCCGGATGC GGCCCCCCAG 
ATGGCGGCCA CCATCACAGT CATGGCCGCC CACCCAGAAG AGGACCACCG GACAATCTAC 
CTGAGCAGCC CAGACTCCTC TGTGGGGGTG CAGTGGCCAC GCGGGCCCCC GAGCCAGCAG 
GCTCCTGAGG CAGGCGGAGA GGAGCCTTCA GCTGGGCAGG GGCTCGGCTC CAGGGAGAGC 
CGGCATCGCA GTGCCAGCGA GACCACGGCC AAGGGGAGAC CTGCCATCCC CCCCAAGATG 
TCGAGGAGCA GCCCTGGAGG GTCCCCGGTA TCCCCCTCCG CCTACCCACT GTCCGACCTC 
AGTGAAGGGA ACTCAGGCAC CCCTGGGCCC CAGCCTCCAT CCAAGGGCCC CGCTGACCCC 
GCTTCTTCCT GCCGGGCCAA TGGCATCCCC TCCAGTGACT CTGCCAGGGG CCCCCCGCCT 
GCCACCAGTA CGTCAGCCTT GGAGCAGAGG CGGCCCCGGC CCCAGGCCGG TGCCTGGAGT 
CGCCAGTGCC GGATTGAGGA GGAGGATGAG GTGGAGCAGG ACTTGCTGAG TCAAAGTTGG 
GGGAGAGAGG TGGAAAATGG CCCCTTGGAC CCGGCCAGCT CCTCCGCCTG GCACCGTCTT 
CGCCCCACAG ATGGCTCCTC TGGGCAGAAC AGCAAAGCTG GGACTGGGAT GAGCAAGTCG 
GCCTCATTTG CCTTTGAGTT CCCCAAGGAC AGAAGCGGGA TTGAGACGTT CTCTCCTCCT 
CCGCCACCTC CAAAGTCACG GCACCTTTTA AAAATGAACA AGAGCAGCTC TGATTTGGAG 
AAGGTGAGCC AGGGCTCTGC CGAGAGCCTC AGCCCGTCCT TCAGGGGCGT CCACGTCAGC 
TTCACCACGG GCTCCACGGA CAGCCTGGCC TCTGACTCCA GGACCTGCAG CGATGGAGGT 
CCATCATCTG AGCCAGCTCA CTCGCCCACC AACAGCAGGA AGAAGCTCTT TGCCCCCGTC 
CCCTTCCCTT CAGGCTCCAC AGAGGATGTG TCCCCCGGCG ACCCCCTGCA GCCCCCGCCT 
CTTCCCCAGA AGAAGCTCGT GAGTCGGGCG GCCTCTTCCC CTGATGGCTT CTTCTGGACC 
CAGGGCTCCC CCAAACCCAG AACAGCAAGT CCCAAGCTGA ACCTCAGCCA CTCGGAGACT 
AACGTCTGTG CCCATGAGGA CTCCCACTTC AGCTACGCCT CAGGCCCTGG CGGCCGCCAC 
CAGCACGTCT TCTCCGCTTC CGAGCCGCTG GAGAAAACTT CCAAAGGCAG TGGCCACCGC 
GGCCCTGTGC TGGGGCTGGG GGGCAGCAAA GGCCTGCAGG GCAAAGGGGT CTCCTCCGCC 
TCGTCCTCTC AGCTGAGCGT GTCCAGCCAG GCCTCGGCGG GCAGCACTCA GCTGCAGCTC 
CACAGCCTCC TGAACAGCAT CAGCAGCAAA GAGGGCACCT ACGCCAAGCT GGGGGGGCTC 
TACGCCCAGT CCCTGGTCCG CCTCGTGGCC AGGTGCGAGG ACCTCTTCAT GGGTGGCCAG 
AAGAAGGAGC TGCACTTCAG CGAGAATAAC TGGTCCCTGT TCAAGCTGAC CTGTAACAAG 
CCCTGCTGTG ACTCGGGGGA TGCCATTTAC TATTGCGCCA CCTGCTCCGA GGACCCCGGC 
AGCACCTACG CCGTGAAGAT CTGCAAGACC CCCGAGCCCA AAGCAGCCTC CTACTGCAGC 
CCTTCAGTGC CCGTGCACTT CAATATCCAG CAGGACTGTG GCCACTTTGT CGCCTCCGTG 
CCCTCCAGCA TGCTCACCTC TCCCGACGCA CACAAGGACC CTCTGTCTGC CCTGCCCTCA 
CATCCCCCCG CCCAGGAGCA GGACTGCGTG GTGGTCATCA CTCGGGAGGT GCCCCACCAA 
ACCGCCTCGG ACTTTGTGCG GGACTTGGCC GCCAGCCACC AGTCTGAGCC GGAGGTTTAC 
GAGCGGCGGG TGTGCTTCCT GCTCCTGCAG CTCTGCAACG GGCTGGAGCA CCTGAAGGAG 
CATGGGATCA TCCACCGAGA CCTGTGCCTG GAGAACCTGC TGCTGGTGCA TTGCGCCCCC 
CAGGCCTCCC CCGAGCCTTC CACCGCTGCC TCCCTCCCTC CCACCACCAC ATCCCCTGCC 
CCTGCGGCTA CCCCCTCCTC TGGCCCCTCA GCCACCGTCC CTACCAGCGT CAACCCCACC 
CCTCCAGCCG CCCCCGCCTT TCAAGGAGGG TCCAGTGAGA AACACTTGCC CCGGCTTATC 
ATCAGCAACT TCTTGAAGGC CAAGCAGAAG CCAGGTGGTA CCACCAACCT GCAGCAGAAG 
AAGAGCCAGG CACGCCTGGC CCCCGAGATC GTGTCGGCCT CCCAGTACCG CAAGTTCGAC 
GAATTCCAAA CGGGCATCCT CATCTACGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGGGCGCAGC TCCGGGAGCA GGACTACCGG CAGGAGGACT TGCCCCCGCT GCCCGCGCTG 
TCCCTCTACT CGCCGGGTCT GCAGCGGCTC GCCCACCTGC TGCTGGAGGC CGACCCCATC 
AAGCGCATCC GCATCGGCGA GGCCAAGCGC GTGCTGCAGT GCCTGCTGTG GGGGCCCCGG 
CGCGAGCTGG TGGAGCCGCC GGGCGCCTCG GAGGAGGTGC TGTGCACCAC GCTCCACAAC 
TGGATCGACA TGAAGCGAGC CCTGATGATG ATGAAGTTCG CCGAGAAGGC GGTGGACCGC 
CGGCGCGGGG TGGAGCTGGA GGACTGGCTC TGCTGCCAGT ATCTGGCGTC GGCCGAGCCC 
ACAGCCCTCT TACAATCACT GAAGCTCCTA CAGCTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316350.1
CDS	227..4168
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_015460864.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460864.1

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GAGAGCTTCC GGCAGAAGCT GGCCGCCTTC 
TCCGGGATGA CTTCCGGCTG CCTCAAGGGC CTGGGGCCCT ACCCGTCTGC TCAGCCCATG 
CGGGAGTCCC TGCCCTCGGA GGATGACAGC GATCAAAGGT GCTCACCCTC GGGGGACAGC 
GAAGGGGGAG AATACTGCTC CATCCTGGAC TGCTGCCCCG GGAGCCCAGG CGCGGAGGAC 
GCCGCCCGGG CCGGCGGTTC CCGACACAGA CAGGGCGGCA GGGACTGCTC GGCAGCCTGC 
TGGGAGCCGC GGGTGTGTGC CAGGCCACCT GAGGAGGAGA AGCAGGCTTT GAGTTTTCCC 
AGGGACTGCT GCAGCCAGGG CCCGACCGAG AGCCCGCCCC GCCTGGGCCC CAAAAAGCAG 
TCCCTCGCTT CAGAGGCTGC CAGCTCTTCG GATGGCCTCT CCTGTGGGAG CGCCAACAGC 
CGCGCCAGCA GTCCCCTGGC CCCCCATCCT GAGAGCGATT ACTGCTCCCT CAGGAAGGAG 
CCTGGGCTGG GGAAGCAGCA GGACTCCGGC TGCCACGGCG TGGCCTCCAG CAGGCGCCTT 
GGGCAGACTG GGGAGACCCA GCCCCCCGCC CACTCCAGGG AGGCTGCTCA GTCTGAACCC 
ATCTATGCTG AGAGTACCAA GAGGAAGAAG GCCGTTCCAG TGCCTTCCAG GCCACAGGCC 
AAGGCAGAAC AGGGGGCAGG CGCTCCGGGC CAGGCCCAGG TGCGGACAGT TAACACCTGG 
GCTCAGAAAG CCGCATCTGG CTGGGGCCAG GTCAGGGAAG GCCCGGATGC GGCCCCCCAG 
ATGGCGGCCA CCATCACAGT CATGGCCGCC CACCCAGAAG AGGACCACCG GACAATCTAC 
CTGAGCAGCC CAGACTCCTC TGTGGGGGTG CAGTGGCCAC GCGGGCCCCC GAGCCAGCAG 
GCTCCTGAGG CAGGCGGAGA GGAGCCTTCA GCTGGGCAGG GGCTCGGCTC CAGGGAGAGC 
CGGCATCGCA GTGCCAGCGA GACCACGGCC AAGGGGAGAC CTGCCATCCC CCCCAAGATG 
TCGAGGAGCA GCCCTGGAGG GTCCCCGGTA TCCCCCTCCG CCTACCCACT GTCCGACCTC 
AGTGAAGGGA ACTCAGGCAC CCCTGGGCCC CAGCCTCCAT CCAAGGGCCC CGCTGACCCC 
GCTTCTTCCT GCCGGGCCAA TGGCATCCCC TCCAGTGACT CTGCCAGGGG CCCCCCGCCT 
GCCACCAGTA CGTCAGCCTT GGAGCAGAGG CGGCCCCGGC CCCAGGCCGG TGCCTGGAGT 
CGCCAGTGCC GGATTGAGGA GGAGGATGAG GTGGAGCAGG ACTTGCTGAG TCAAAGTTGG 
GGGAGAGAGG TGGAAAATGG CCCCTTGGAC CCGGCCAGCT CCTCCGCCTG GCACCGTCTT 
CGCCCCACAG ATGGCTCCTC TGGGCAGAAC AGCAAAGCTG GGACTGGGAT GAGCAAGTCG 
GCCTCATTTG CCTTTGAGTT CCCCAAGGAC AGAAGCGGGA TTGAGACGTT CTCTCCTCCT 
CCGCCACCTC CAAAGTCACG GCACCTTTTA AAAATGAACA AGAGCAGCTC TGATTTGGAG 
AAGGTGAGCC AGGGCTCTGC CGAGAGCCTC AGCCCGTCCT TCAGGGGCGT CCACGTCAGC 
TTCACCACGG GCTCCACGGA CAGCCTGGCC TCTGACTCCA GGACCTGCAG CGATGGAGGT 
CCATCATCTG AGCCAGCTCA CTCGCCCACC AACAGCAGGA AGAAGCTCTT TGCCCCCGTC 
CCCTTCCCTT CAGGCTCCAC AGAGGATGTG TCCCCCGGCG ACCCCCTGCA GCCCCCGCCT 
CTTCCCCAGA AGAAGCTCGT GAGTCGGGCG GCCTCTTCCC CTGATGGCTT CTTCTGGACC 
CAGGGCTCCC CCAAACCCAG AACAGCAAGT CCCAAGCTGA ACCTCAGCCA CTCGGAGACT 
AACGTCTGTG CCCATGAGGA CTCCCACTTC AGCTACGCCT CAGGCCCTGG CGGCCGCCAC 
CAGCACGTCT TCTCCGCTTC CGAGCCGCTG GAGAAAACTT CCAAAGGCAG TGGCCACCGC 
GGCCCTGTGC TGGGGCTGGG GGGCAGCAAA GGCCTGCAGG GCAAAGGGGT CTCCTCCGCC 
TCGTCCTCTC AGCTGAGCGT GTCCAGCCAG GCCTCGGCGG GCAGCACTCA GCTGCAGCTC 
CACAGCCTCC TGAACAGCAT CAGCAGCAAA GAGGGCACCT ACGCCAAGCT GGGGGGGCTC 
TACGCCCAGT CCCTGGTCCG CCTCGTGGCC AGGTGCGAGG ACCTCTTCAT GGGTGGCCAG 
AAGAAGGAGC TGCACTTCAG CGAGAATAAC TGGTCCCTGT TCAAGCTGAC CTGTAACAAG 
CCCTGCTGTG ACTCGGGGGA TGCCATTTAC TATTGCGCCA CCTGCTCCGA GGACCCCGGC 
AGCACCTACG CCGTGAAGAT CTGCAAGACC CCCGAGCCCA AAGCAGCCTC CTACTGCAGC 
CCTTCAGTGC CCGTGCACTT CAATATCCAG CAGGACTGTG GCCACTTTGT CGCCTCCGTG 
CCCTCCAGCA TGCTCACCTC TCCCGACGCA CACAAGGACC CTCTGTCTGC CCTGCCCTCA 
CATCCCCCCG CCCAGGAGCA GGACTGCGTG GTGGTCATCA CTCGGGAGGT GCCCCACCAA 
ACCGCCTCGG ACTTTGTGCG GGACTTGGCC GCCAGCCACC AGTCTGAGCC GGAGGTTTAC 
GAGCGGCGGG TGTGCTTCCT GCTCCTGCAG CTCTGCAACG GGCTGGAGCA CCTGAAGGAG 
CATGGGATCA TCCACCGAGA CCTGTGCCTG GAGAACCTGC TGCTGGTGCA TTGCGCCCCC 
CAGGCCTCCC CCGAGCCTTC CACCGCTGCC TCCCTCCCTC CCACCACCAC ATCCCCTGCC 
CCTGCGGCTA CCCCCTCCTC TGGCCCCTCA GCCACCGTCC CTACCAGCGT CAACCCCACC 
CCTCCAGCCG CCCCCGCCTT TCAAGGAGGG TCCAGTGAGA AACACTTGCC CCGGCTTATC 
ATCAGCAACT TCTTGAAGGC CAAGCAGAAG CCAGGTGGTA CCACCAACCT GCAGCAGAAG 
AAGAGCCAGG CACGCCTGGC CCCCGAGATC GTGTCGGCCT CCCAGTACCG CAAGTTCGAC 
GAATTCCAAA CGGGCATCCT CATCTACGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGGGCGCAGC TCCGGGAGCA GGACTACCGG CAGGAGGACT TGCCCCCGCT GCCCGCGCTG 
TCCCTCTACT CGCCGGGTCT GCAGCGGCTC GCCCACCTGC TGCTGGAGGC CGACCCCATC 
AAGCGCATCC GCATCGGCGA GGCCAAGCGC GTGCTGCAGT GCCTGCTGTG GGGGCCCCGG 
CGCGAGCTGG TGGAGCCGCC GGGCGCCTCG GAGGAGGTGC TGTGCACCAC GCTCCACAAC 
TGGATCGACA TGAAGCGAGC CCTGATGATG ATGAAGTTCG CCGAGAAGGC GGTGGACCGC 
CGGCGCGGGG TGGAGCTGGA GGACTGGCTC TGCTGCCAGT ATCTGGCGTC GGCCGAGCCC 
ACAGCCCTCT TACAATCACT GAAGCTCCTA CAGCTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_015469737.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325223.1 Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X4, mRNA.

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CloneID	OBa88845
Clone ID Related Accession (Same CDS sequence)	XM_015460864.1 , XM_015469737.1 , XM_015460864.2
Accession Version	XM_015469737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase SgK223 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646720.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GAGAGCTTCC GGCAGAAGCT GGCCGCCTTC 
TCCGGGATGA CTTCCGGCTG CCTCAAGGGC CTGGGGCCCT ACCCGTCTGC TCAGCCCATG 
CGGGAGTCCC TGCCCTCGGA GGATGACAGC GATCAAAGGT GCTCACCCTC GGGGGACAGC 
GAAGGGGGAG AATACTGCTC CATCCTGGAC TGCTGCCCCG GGAGCCCAGG CGCGGAGGAC 
GCCGCCCGGG CCGGCGGTTC CCGACACAGA CAGGGCGGCA GGGACTGCTC GGCAGCCTGC 
TGGGAGCCGC GGGTGTGTGC CAGGCCACCT GAGGAGGAGA AGCAGGCTTT GAGTTTTCCC 
AGGGACTGCT GCAGCCAGGG CCCGACCGAG AGCCCGCCCC GCCTGGGCCC CAAAAAGCAG 
TCCCTCGCTT CAGAGGCTGC CAGCTCTTCG GATGGCCTCT CCTGTGGGAG CGCCAACAGC 
CGCGCCAGCA GTCCCCTGGC CCCCCATCCT GAGAGCGATT ACTGCTCCCT CAGGAAGGAG 
CCTGGGCTGG GGAAGCAGCA GGACTCCGGC TGCCACGGCG TGGCCTCCAG CAGGCGCCTT 
GGGCAGACTG GGGAGACCCA GCCCCCCGCC CACTCCAGGG AGGCTGCTCA GTCTGAACCC 
ATCTATGCTG AGAGTACCAA GAGGAAGAAG GCCGTTCCAG TGCCTTCCAG GCCACAGGCC 
AAGGCAGAAC AGGGGGCAGG CGCTCCGGGC CAGGCCCAGG TGCGGACAGT TAACACCTGG 
GCTCAGAAAG CCGCATCTGG CTGGGGCCAG GTCAGGGAAG GCCCGGATGC GGCCCCCCAG 
ATGGCGGCCA CCATCACAGT CATGGCCGCC CACCCAGAAG AGGACCACCG GACAATCTAC 
CTGAGCAGCC CAGACTCCTC TGTGGGGGTG CAGTGGCCAC GCGGGCCCCC GAGCCAGCAG 
GCTCCTGAGG CAGGCGGAGA GGAGCCTTCA GCTGGGCAGG GGCTCGGCTC CAGGGAGAGC 
CGGCATCGCA GTGCCAGCGA GACCACGGCC AAGGGGAGAC CTGCCATCCC CCCCAAGATG 
TCGAGGAGCA GCCCTGGAGG GTCCCCGGTA TCCCCCTCCG CCTACCCACT GTCCGACCTC 
AGTGAAGGGA ACTCAGGCAC CCCTGGGCCC CAGCCTCCAT CCAAGGGCCC CGCTGACCCC 
GCTTCTTCCT GCCGGGCCAA TGGCATCCCC TCCAGTGACT CTGCCAGGGG CCCCCCGCCT 
GCCACCAGTA CGTCAGCCTT GGAGCAGAGG CGGCCCCGGC CCCAGGCCGG TGCCTGGAGT 
CGCCAGTGCC GGATTGAGGA GGAGGATGAG GTGGAGCAGG ACTTGCTGAG TCAAAGTTGG 
GGGAGAGAGG TGGAAAATGG CCCCTTGGAC CCGGCCAGCT CCTCCGCCTG GCACCGTCTT 
CGCCCCACAG ATGGCTCCTC TGGGCAGAAC AGCAAAGCTG GGACTGGGAT GAGCAAGTCG 
GCCTCATTTG CCTTTGAGTT CCCCAAGGAC AGAAGCGGGA TTGAGACGTT CTCTCCTCCT 
CCGCCACCTC CAAAGTCACG GCACCTTTTA AAAATGAACA AGAGCAGCTC TGATTTGGAG 
AAGGTGAGCC AGGGCTCTGC CGAGAGCCTC AGCCCGTCCT TCAGGGGCGT CCACGTCAGC 
TTCACCACGG GCTCCACGGA CAGCCTGGCC TCTGACTCCA GGACCTGCAG CGATGGAGGT 
CCATCATCTG AGCCAGCTCA CTCGCCCACC AACAGCAGGA AGAAGCTCTT TGCCCCCGTC 
CCCTTCCCTT CAGGCTCCAC AGAGGATGTG TCCCCCGGCG ACCCCCTGCA GCCCCCGCCT 
CTTCCCCAGA AGAAGCTCGT GAGTCGGGCG GCCTCTTCCC CTGATGGCTT CTTCTGGACC 
CAGGGCTCCC CCAAACCCAG AACAGCAAGT CCCAAGCTGA ACCTCAGCCA CTCGGAGACT 
AACGTCTGTG CCCATGAGGA CTCCCACTTC AGCTACGCCT CAGGCCCTGG CGGCCGCCAC 
CAGCACGTCT TCTCCGCTTC CGAGCCGCTG GAGAAAACTT CCAAAGGCAG TGGCCACCGC 
GGCCCTGTGC TGGGGCTGGG GGGCAGCAAA GGCCTGCAGG GCAAAGGGGT CTCCTCCGCC 
TCGTCCTCTC AGCTGAGCGT GTCCAGCCAG GCCTCGGCGG GCAGCACTCA GCTGCAGCTC 
CACAGCCTCC TGAACAGCAT CAGCAGCAAA GAGGGCACCT ACGCCAAGCT GGGGGGGCTC 
TACGCCCAGT CCCTGGTCCG CCTCGTGGCC AGGTGCGAGG ACCTCTTCAT GGGTGGCCAG 
AAGAAGGAGC TGCACTTCAG CGAGAATAAC TGGTCCCTGT TCAAGCTGAC CTGTAACAAG 
CCCTGCTGTG ACTCGGGGGA TGCCATTTAC TATTGCGCCA CCTGCTCCGA GGACCCCGGC 
AGCACCTACG CCGTGAAGAT CTGCAAGACC CCCGAGCCCA AAGCAGCCTC CTACTGCAGC 
CCTTCAGTGC CCGTGCACTT CAATATCCAG CAGGACTGTG GCCACTTTGT CGCCTCCGTG 
CCCTCCAGCA TGCTCACCTC TCCCGACGCA CACAAGGACC CTCTGTCTGC CCTGCCCTCA 
CATCCCCCCG CCCAGGAGCA GGACTGCGTG GTGGTCATCA CTCGGGAGGT GCCCCACCAA 
ACCGCCTCGG ACTTTGTGCG GGACTTGGCC GCCAGCCACC AGTCTGAGCC GGAGGTTTAC 
GAGCGGCGGG TGTGCTTCCT GCTCCTGCAG CTCTGCAACG GGCTGGAGCA CCTGAAGGAG 
CATGGGATCA TCCACCGAGA CCTGTGCCTG GAGAACCTGC TGCTGGTGCA TTGCGCCCCC 
CAGGCCTCCC CCGAGCCTTC CACCGCTGCC TCCCTCCCTC CCACCACCAC ATCCCCTGCC 
CCTGCGGCTA CCCCCTCCTC TGGCCCCTCA GCCACCGTCC CTACCAGCGT CAACCCCACC 
CCTCCAGCCG CCCCCGCCTT TCAAGGAGGG TCCAGTGAGA AACACTTGCC CCGGCTTATC 
ATCAGCAACT TCTTGAAGGC CAAGCAGAAG CCAGGTGGTA CCACCAACCT GCAGCAGAAG 
AAGAGCCAGG CACGCCTGGC CCCCGAGATC GTGTCGGCCT CCCAGTACCG CAAGTTCGAC 
GAATTCCAAA CGGGCATCCT CATCTACGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGGGCGCAGC TCCGGGAGCA GGACTACCGG CAGGAGGACT TGCCCCCGCT GCCCGCGCTG 
TCCCTCTACT CGCCGGGTCT GCAGCGGCTC GCCCACCTGC TGCTGGAGGC CGACCCCATC 
AAGCGCATCC GCATCGGCGA GGCCAAGCGC GTGCTGCAGT GCCTGCTGTG GGGGCCCCGG 
CGCGAGCTGG TGGAGCCGCC GGGCGCCTCG GAGGAGGTGC TGTGCACCAC GCTCCACAAC 
TGGATCGACA TGAAGCGAGC CCTGATGATG ATGAAGTTCG CCGAGAAGGC GGTGGACCGC 
CGGCGCGGGG TGGAGCTGGA GGACTGGCTC TGCTGCCAGT ATCTGGCGTC GGCCGAGCCC 
ACAGCCCTCT TACAATCACT GAAGCTCCTA CAGCTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325223.1
CDS	227..4168
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_015469737.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine-protein kinase SgK223 (LOC533821), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469737.1

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GAGAGCTTCC GGCAGAAGCT GGCCGCCTTC 
TCCGGGATGA CTTCCGGCTG CCTCAAGGGC CTGGGGCCCT ACCCGTCTGC TCAGCCCATG 
CGGGAGTCCC TGCCCTCGGA GGATGACAGC GATCAAAGGT GCTCACCCTC GGGGGACAGC 
GAAGGGGGAG AATACTGCTC CATCCTGGAC TGCTGCCCCG GGAGCCCAGG CGCGGAGGAC 
GCCGCCCGGG CCGGCGGTTC CCGACACAGA CAGGGCGGCA GGGACTGCTC GGCAGCCTGC 
TGGGAGCCGC GGGTGTGTGC CAGGCCACCT GAGGAGGAGA AGCAGGCTTT GAGTTTTCCC 
AGGGACTGCT GCAGCCAGGG CCCGACCGAG AGCCCGCCCC GCCTGGGCCC CAAAAAGCAG 
TCCCTCGCTT CAGAGGCTGC CAGCTCTTCG GATGGCCTCT CCTGTGGGAG CGCCAACAGC 
CGCGCCAGCA GTCCCCTGGC CCCCCATCCT GAGAGCGATT ACTGCTCCCT CAGGAAGGAG 
CCTGGGCTGG GGAAGCAGCA GGACTCCGGC TGCCACGGCG TGGCCTCCAG CAGGCGCCTT 
GGGCAGACTG GGGAGACCCA GCCCCCCGCC CACTCCAGGG AGGCTGCTCA GTCTGAACCC 
ATCTATGCTG AGAGTACCAA GAGGAAGAAG GCCGTTCCAG TGCCTTCCAG GCCACAGGCC 
AAGGCAGAAC AGGGGGCAGG CGCTCCGGGC CAGGCCCAGG TGCGGACAGT TAACACCTGG 
GCTCAGAAAG CCGCATCTGG CTGGGGCCAG GTCAGGGAAG GCCCGGATGC GGCCCCCCAG 
ATGGCGGCCA CCATCACAGT CATGGCCGCC CACCCAGAAG AGGACCACCG GACAATCTAC 
CTGAGCAGCC CAGACTCCTC TGTGGGGGTG CAGTGGCCAC GCGGGCCCCC GAGCCAGCAG 
GCTCCTGAGG CAGGCGGAGA GGAGCCTTCA GCTGGGCAGG GGCTCGGCTC CAGGGAGAGC 
CGGCATCGCA GTGCCAGCGA GACCACGGCC AAGGGGAGAC CTGCCATCCC CCCCAAGATG 
TCGAGGAGCA GCCCTGGAGG GTCCCCGGTA TCCCCCTCCG CCTACCCACT GTCCGACCTC 
AGTGAAGGGA ACTCAGGCAC CCCTGGGCCC CAGCCTCCAT CCAAGGGCCC CGCTGACCCC 
GCTTCTTCCT GCCGGGCCAA TGGCATCCCC TCCAGTGACT CTGCCAGGGG CCCCCCGCCT 
GCCACCAGTA CGTCAGCCTT GGAGCAGAGG CGGCCCCGGC CCCAGGCCGG TGCCTGGAGT 
CGCCAGTGCC GGATTGAGGA GGAGGATGAG GTGGAGCAGG ACTTGCTGAG TCAAAGTTGG 
GGGAGAGAGG TGGAAAATGG CCCCTTGGAC CCGGCCAGCT CCTCCGCCTG GCACCGTCTT 
CGCCCCACAG ATGGCTCCTC TGGGCAGAAC AGCAAAGCTG GGACTGGGAT GAGCAAGTCG 
GCCTCATTTG CCTTTGAGTT CCCCAAGGAC AGAAGCGGGA TTGAGACGTT CTCTCCTCCT 
CCGCCACCTC CAAAGTCACG GCACCTTTTA AAAATGAACA AGAGCAGCTC TGATTTGGAG 
AAGGTGAGCC AGGGCTCTGC CGAGAGCCTC AGCCCGTCCT TCAGGGGCGT CCACGTCAGC 
TTCACCACGG GCTCCACGGA CAGCCTGGCC TCTGACTCCA GGACCTGCAG CGATGGAGGT 
CCATCATCTG AGCCAGCTCA CTCGCCCACC AACAGCAGGA AGAAGCTCTT TGCCCCCGTC 
CCCTTCCCTT CAGGCTCCAC AGAGGATGTG TCCCCCGGCG ACCCCCTGCA GCCCCCGCCT 
CTTCCCCAGA AGAAGCTCGT GAGTCGGGCG GCCTCTTCCC CTGATGGCTT CTTCTGGACC 
CAGGGCTCCC CCAAACCCAG AACAGCAAGT CCCAAGCTGA ACCTCAGCCA CTCGGAGACT 
AACGTCTGTG CCCATGAGGA CTCCCACTTC AGCTACGCCT CAGGCCCTGG CGGCCGCCAC 
CAGCACGTCT TCTCCGCTTC CGAGCCGCTG GAGAAAACTT CCAAAGGCAG TGGCCACCGC 
GGCCCTGTGC TGGGGCTGGG GGGCAGCAAA GGCCTGCAGG GCAAAGGGGT CTCCTCCGCC 
TCGTCCTCTC AGCTGAGCGT GTCCAGCCAG GCCTCGGCGG GCAGCACTCA GCTGCAGCTC 
CACAGCCTCC TGAACAGCAT CAGCAGCAAA GAGGGCACCT ACGCCAAGCT GGGGGGGCTC 
TACGCCCAGT CCCTGGTCCG CCTCGTGGCC AGGTGCGAGG ACCTCTTCAT GGGTGGCCAG 
AAGAAGGAGC TGCACTTCAG CGAGAATAAC TGGTCCCTGT TCAAGCTGAC CTGTAACAAG 
CCCTGCTGTG ACTCGGGGGA TGCCATTTAC TATTGCGCCA CCTGCTCCGA GGACCCCGGC 
AGCACCTACG CCGTGAAGAT CTGCAAGACC CCCGAGCCCA AAGCAGCCTC CTACTGCAGC 
CCTTCAGTGC CCGTGCACTT CAATATCCAG CAGGACTGTG GCCACTTTGT CGCCTCCGTG 
CCCTCCAGCA TGCTCACCTC TCCCGACGCA CACAAGGACC CTCTGTCTGC CCTGCCCTCA 
CATCCCCCCG CCCAGGAGCA GGACTGCGTG GTGGTCATCA CTCGGGAGGT GCCCCACCAA 
ACCGCCTCGG ACTTTGTGCG GGACTTGGCC GCCAGCCACC AGTCTGAGCC GGAGGTTTAC 
GAGCGGCGGG TGTGCTTCCT GCTCCTGCAG CTCTGCAACG GGCTGGAGCA CCTGAAGGAG 
CATGGGATCA TCCACCGAGA CCTGTGCCTG GAGAACCTGC TGCTGGTGCA TTGCGCCCCC 
CAGGCCTCCC CCGAGCCTTC CACCGCTGCC TCCCTCCCTC CCACCACCAC ATCCCCTGCC 
CCTGCGGCTA CCCCCTCCTC TGGCCCCTCA GCCACCGTCC CTACCAGCGT CAACCCCACC 
CCTCCAGCCG CCCCCGCCTT TCAAGGAGGG TCCAGTGAGA AACACTTGCC CCGGCTTATC 
ATCAGCAACT TCTTGAAGGC CAAGCAGAAG CCAGGTGGTA CCACCAACCT GCAGCAGAAG 
AAGAGCCAGG CACGCCTGGC CCCCGAGATC GTGTCGGCCT CCCAGTACCG CAAGTTCGAC 
GAATTCCAAA CGGGCATCCT CATCTACGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGGGCGCAGC TCCGGGAGCA GGACTACCGG CAGGAGGACT TGCCCCCGCT GCCCGCGCTG 
TCCCTCTACT CGCCGGGTCT GCAGCGGCTC GCCCACCTGC TGCTGGAGGC CGACCCCATC 
AAGCGCATCC GCATCGGCGA GGCCAAGCGC GTGCTGCAGT GCCTGCTGTG GGGGCCCCGG 
CGCGAGCTGG TGGAGCCGCC GGGCGCCTCG GAGGAGGTGC TGTGCACCAC GCTCCACAAC 
TGGATCGACA TGAAGCGAGC CCTGATGATG ATGAAGTTCG CCGAGAAGGC GGTGGACCGC 
CGGCGCGGGG TGGAGCTGGA GGACTGGCTC TGCTGCCAGT ATCTGGCGTC GGCCGAGCCC 
ACAGCCCTCT TACAATCACT GAAGCTCCTA CAGCTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRAG1 ( XM_015460864.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316350.1 Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X3, mRNA.

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CloneID	OBa88845
Clone ID Related Accession (Same CDS sequence)	XM_015460864.1 , XM_015469737.1 , XM_015460864.2
Accession Version	XM_015460864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase PRAG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GAGAGCTTCC GGCAGAAGCT GGCCGCCTTC 
TCCGGGATGA CTTCCGGCTG CCTCAAGGGC CTGGGGCCCT ACCCGTCTGC TCAGCCCATG 
CGGGAGTCCC TGCCCTCGGA GGATGACAGC GATCAAAGGT GCTCACCCTC GGGGGACAGC 
GAAGGGGGAG AATACTGCTC CATCCTGGAC TGCTGCCCCG GGAGCCCAGG CGCGGAGGAC 
GCCGCCCGGG CCGGCGGTTC CCGACACAGA CAGGGCGGCA GGGACTGCTC GGCAGCCTGC 
TGGGAGCCGC GGGTGTGTGC CAGGCCACCT GAGGAGGAGA AGCAGGCTTT GAGTTTTCCC 
AGGGACTGCT GCAGCCAGGG CCCGACCGAG AGCCCGCCCC GCCTGGGCCC CAAAAAGCAG 
TCCCTCGCTT CAGAGGCTGC CAGCTCTTCG GATGGCCTCT CCTGTGGGAG CGCCAACAGC 
CGCGCCAGCA GTCCCCTGGC CCCCCATCCT GAGAGCGATT ACTGCTCCCT CAGGAAGGAG 
CCTGGGCTGG GGAAGCAGCA GGACTCCGGC TGCCACGGCG TGGCCTCCAG CAGGCGCCTT 
GGGCAGACTG GGGAGACCCA GCCCCCCGCC CACTCCAGGG AGGCTGCTCA GTCTGAACCC 
ATCTATGCTG AGAGTACCAA GAGGAAGAAG GCCGTTCCAG TGCCTTCCAG GCCACAGGCC 
AAGGCAGAAC AGGGGGCAGG CGCTCCGGGC CAGGCCCAGG TGCGGACAGT TAACACCTGG 
GCTCAGAAAG CCGCATCTGG CTGGGGCCAG GTCAGGGAAG GCCCGGATGC GGCCCCCCAG 
ATGGCGGCCA CCATCACAGT CATGGCCGCC CACCCAGAAG AGGACCACCG GACAATCTAC 
CTGAGCAGCC CAGACTCCTC TGTGGGGGTG CAGTGGCCAC GCGGGCCCCC GAGCCAGCAG 
GCTCCTGAGG CAGGCGGAGA GGAGCCTTCA GCTGGGCAGG GGCTCGGCTC CAGGGAGAGC 
CGGCATCGCA GTGCCAGCGA GACCACGGCC AAGGGGAGAC CTGCCATCCC CCCCAAGATG 
TCGAGGAGCA GCCCTGGAGG GTCCCCGGTA TCCCCCTCCG CCTACCCACT GTCCGACCTC 
AGTGAAGGGA ACTCAGGCAC CCCTGGGCCC CAGCCTCCAT CCAAGGGCCC CGCTGACCCC 
GCTTCTTCCT GCCGGGCCAA TGGCATCCCC TCCAGTGACT CTGCCAGGGG CCCCCCGCCT 
GCCACCAGTA CGTCAGCCTT GGAGCAGAGG CGGCCCCGGC CCCAGGCCGG TGCCTGGAGT 
CGCCAGTGCC GGATTGAGGA GGAGGATGAG GTGGAGCAGG ACTTGCTGAG TCAAAGTTGG 
GGGAGAGAGG TGGAAAATGG CCCCTTGGAC CCGGCCAGCT CCTCCGCCTG GCACCGTCTT 
CGCCCCACAG ATGGCTCCTC TGGGCAGAAC AGCAAAGCTG GGACTGGGAT GAGCAAGTCG 
GCCTCATTTG CCTTTGAGTT CCCCAAGGAC AGAAGCGGGA TTGAGACGTT CTCTCCTCCT 
CCGCCACCTC CAAAGTCACG GCACCTTTTA AAAATGAACA AGAGCAGCTC TGATTTGGAG 
AAGGTGAGCC AGGGCTCTGC CGAGAGCCTC AGCCCGTCCT TCAGGGGCGT CCACGTCAGC 
TTCACCACGG GCTCCACGGA CAGCCTGGCC TCTGACTCCA GGACCTGCAG CGATGGAGGT 
CCATCATCTG AGCCAGCTCA CTCGCCCACC AACAGCAGGA AGAAGCTCTT TGCCCCCGTC 
CCCTTCCCTT CAGGCTCCAC AGAGGATGTG TCCCCCGGCG ACCCCCTGCA GCCCCCGCCT 
CTTCCCCAGA AGAAGCTCGT GAGTCGGGCG GCCTCTTCCC CTGATGGCTT CTTCTGGACC 
CAGGGCTCCC CCAAACCCAG AACAGCAAGT CCCAAGCTGA ACCTCAGCCA CTCGGAGACT 
AACGTCTGTG CCCATGAGGA CTCCCACTTC AGCTACGCCT CAGGCCCTGG CGGCCGCCAC 
CAGCACGTCT TCTCCGCTTC CGAGCCGCTG GAGAAAACTT CCAAAGGCAG TGGCCACCGC 
GGCCCTGTGC TGGGGCTGGG GGGCAGCAAA GGCCTGCAGG GCAAAGGGGT CTCCTCCGCC 
TCGTCCTCTC AGCTGAGCGT GTCCAGCCAG GCCTCGGCGG GCAGCACTCA GCTGCAGCTC 
CACAGCCTCC TGAACAGCAT CAGCAGCAAA GAGGGCACCT ACGCCAAGCT GGGGGGGCTC 
TACGCCCAGT CCCTGGTCCG CCTCGTGGCC AGGTGCGAGG ACCTCTTCAT GGGTGGCCAG 
AAGAAGGAGC TGCACTTCAG CGAGAATAAC TGGTCCCTGT TCAAGCTGAC CTGTAACAAG 
CCCTGCTGTG ACTCGGGGGA TGCCATTTAC TATTGCGCCA CCTGCTCCGA GGACCCCGGC 
AGCACCTACG CCGTGAAGAT CTGCAAGACC CCCGAGCCCA AAGCAGCCTC CTACTGCAGC 
CCTTCAGTGC CCGTGCACTT CAATATCCAG CAGGACTGTG GCCACTTTGT CGCCTCCGTG 
CCCTCCAGCA TGCTCACCTC TCCCGACGCA CACAAGGACC CTCTGTCTGC CCTGCCCTCA 
CATCCCCCCG CCCAGGAGCA GGACTGCGTG GTGGTCATCA CTCGGGAGGT GCCCCACCAA 
ACCGCCTCGG ACTTTGTGCG GGACTTGGCC GCCAGCCACC AGTCTGAGCC GGAGGTTTAC 
GAGCGGCGGG TGTGCTTCCT GCTCCTGCAG CTCTGCAACG GGCTGGAGCA CCTGAAGGAG 
CATGGGATCA TCCACCGAGA CCTGTGCCTG GAGAACCTGC TGCTGGTGCA TTGCGCCCCC 
CAGGCCTCCC CCGAGCCTTC CACCGCTGCC TCCCTCCCTC CCACCACCAC ATCCCCTGCC 
CCTGCGGCTA CCCCCTCCTC TGGCCCCTCA GCCACCGTCC CTACCAGCGT CAACCCCACC 
CCTCCAGCCG CCCCCGCCTT TCAAGGAGGG TCCAGTGAGA AACACTTGCC CCGGCTTATC 
ATCAGCAACT TCTTGAAGGC CAAGCAGAAG CCAGGTGGTA CCACCAACCT GCAGCAGAAG 
AAGAGCCAGG CACGCCTGGC CCCCGAGATC GTGTCGGCCT CCCAGTACCG CAAGTTCGAC 
GAATTCCAAA CGGGCATCCT CATCTACGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGGGCGCAGC TCCGGGAGCA GGACTACCGG CAGGAGGACT TGCCCCCGCT GCCCGCGCTG 
TCCCTCTACT CGCCGGGTCT GCAGCGGCTC GCCCACCTGC TGCTGGAGGC CGACCCCATC 
AAGCGCATCC GCATCGGCGA GGCCAAGCGC GTGCTGCAGT GCCTGCTGTG GGGGCCCCGG 
CGCGAGCTGG TGGAGCCGCC GGGCGCCTCG GAGGAGGTGC TGTGCACCAC GCTCCACAAC 
TGGATCGACA TGAAGCGAGC CCTGATGATG ATGAAGTTCG CCGAGAAGGC GGTGGACCGC 
CGGCGCGGGG TGGAGCTGGA GGACTGGCTC TGCTGCCAGT ATCTGGCGTC GGCCGAGCCC 
ACAGCCCTCT TACAATCACT GAAGCTCCTA CAGCTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316350.1
CDS	241..4182
Translation	MLETIPLNPESPKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVPGRPQPRVGCLPPPPRLHPRPESDRPEDDGLSGSPYSKPTIAVKPTMMSSEASDGWTEAGMSADVAQESFRQKLAAFSGMTSGCLKGLGPYPSAQPMRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPGAEDAARAGGSRHRQGGRDCSAACWEPRVCARPPEEEKQALSFPRDCCSQGPTESPPRLGPKKQSLASEAASSSDGLSCGSANSRASSPLAPHPESDYCSLRKEPGLGKQQDSGCHGVASSRRLGQTGETQPPAHSREAAQSEPIYAESTKRKKAVPVPSRPQAKAEQGAGAPGQAQVRTVNTWAQKAASGWGQVREGPDAAPQMAATITVMAAHPEEDHRTIYLSSPDSSVGVQWPRGPPSQQAPEAGGEEPSAGQGLGSRESRHRSASETTAKGRPAIPPKMSRSSPGGSPVSPSAYPLSDLSEGNSGTPGPQPPSKGPADPASSCRANGIPSSDSARGPPPATSTSALEQRRPRPQAGAWSRQCRIEEEDEVEQDLLSQSWGREVENGPLDPASSSAWHRLRPTDGSSGQNSKAGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSEPAHSPTNSRKKLFAPVPFPSGSTEDVSPGDPLQPPPLPQKKLVSRAASSPDGFFWTQGSPKPRTASPKLNLSHSETNVCAHEDSHFSYASGPGGRHQHVFSASEPLEKTSKGSGHRGPVLGLGGSKGLQGKGVSSASSSQLSVSSQASAGSTQLQLHSLLNSISSKEGTYAKLGGLYAQSLVRLVARCEDLFMGGQKKELHFSENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKTPEPKAASYCSPSVPVHFNIQQDCGHFVASVPSSMLTSPDAHKDPLSALPSHPPAQEQDCVVVITREVPHQTASDFVRDLAASHQSEPEVYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCAPQASPEPSTAASLPPTTTSPAPAATPSSGPSATVPTSVNPTPPAAPAFQGGSSEKHLPRLIISNFLKAKQKPGGTTNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLREQDYRQEDLPPLPALSLYSPGLQRLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVEPPGASEEVLCTTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPTALLQSLKLLQLL

Target ORF information:

RefSeq Version	XM_015460864.2
Organism	Bos taurus(cattle)
Definition	Bos taurus PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460864.2

ORF Insert Sequence:

ATGCTCGAGA CCATTCCACT GAACCCCGAG AGCCCGAAGA TGTCTGCGTG CAGCGACTTT 
GTGGAGCACA TCTGGAAGCC CGGGTCCTGC AAAAACTGCT TCTGCCTGCG CAGCGACCAT 
CAACTGGTGC CTGGCCGCCC CCAGCCCCGA GTTGGCTGCC TGCCCCCTCC GCCTCGCCTG 
CACCCCAGGC CGGAGAGCGA CCGCCCGGAA GATGACGGCC TGAGCGGTTC GCCCTACTCC 
AAGCCCACCA TTGCCGTGAA GCCCACCATG ATGAGCTCTG AGGCCTCCGA TGGGTGGACA 
GAGGCCGGCA TGAGTGCCGA CGTCGCACAG GAGAGCTTCC GGCAGAAGCT GGCCGCCTTC 
TCCGGGATGA CTTCCGGCTG CCTCAAGGGC CTGGGGCCCT ACCCGTCTGC TCAGCCCATG 
CGGGAGTCCC TGCCCTCGGA GGATGACAGC GATCAAAGGT GCTCACCCTC GGGGGACAGC 
GAAGGGGGAG AATACTGCTC CATCCTGGAC TGCTGCCCCG GGAGCCCAGG CGCGGAGGAC 
GCCGCCCGGG CCGGCGGTTC CCGACACAGA CAGGGCGGCA GGGACTGCTC GGCAGCCTGC 
TGGGAGCCGC GGGTGTGTGC CAGGCCACCT GAGGAGGAGA AGCAGGCTTT GAGTTTTCCC 
AGGGACTGCT GCAGCCAGGG CCCGACCGAG AGCCCGCCCC GCCTGGGCCC CAAAAAGCAG 
TCCCTCGCTT CAGAGGCTGC CAGCTCTTCG GATGGCCTCT CCTGTGGGAG CGCCAACAGC 
CGCGCCAGCA GTCCCCTGGC CCCCCATCCT GAGAGCGATT ACTGCTCCCT CAGGAAGGAG 
CCTGGGCTGG GGAAGCAGCA GGACTCCGGC TGCCACGGCG TGGCCTCCAG CAGGCGCCTT 
GGGCAGACTG GGGAGACCCA GCCCCCCGCC CACTCCAGGG AGGCTGCTCA GTCTGAACCC 
ATCTATGCTG AGAGTACCAA GAGGAAGAAG GCCGTTCCAG TGCCTTCCAG GCCACAGGCC 
AAGGCAGAAC AGGGGGCAGG CGCTCCGGGC CAGGCCCAGG TGCGGACAGT TAACACCTGG 
GCTCAGAAAG CCGCATCTGG CTGGGGCCAG GTCAGGGAAG GCCCGGATGC GGCCCCCCAG 
ATGGCGGCCA CCATCACAGT CATGGCCGCC CACCCAGAAG AGGACCACCG GACAATCTAC 
CTGAGCAGCC CAGACTCCTC TGTGGGGGTG CAGTGGCCAC GCGGGCCCCC GAGCCAGCAG 
GCTCCTGAGG CAGGCGGAGA GGAGCCTTCA GCTGGGCAGG GGCTCGGCTC CAGGGAGAGC 
CGGCATCGCA GTGCCAGCGA GACCACGGCC AAGGGGAGAC CTGCCATCCC CCCCAAGATG 
TCGAGGAGCA GCCCTGGAGG GTCCCCGGTA TCCCCCTCCG CCTACCCACT GTCCGACCTC 
AGTGAAGGGA ACTCAGGCAC CCCTGGGCCC CAGCCTCCAT CCAAGGGCCC CGCTGACCCC 
GCTTCTTCCT GCCGGGCCAA TGGCATCCCC TCCAGTGACT CTGCCAGGGG CCCCCCGCCT 
GCCACCAGTA CGTCAGCCTT GGAGCAGAGG CGGCCCCGGC CCCAGGCCGG TGCCTGGAGT 
CGCCAGTGCC GGATTGAGGA GGAGGATGAG GTGGAGCAGG ACTTGCTGAG TCAAAGTTGG 
GGGAGAGAGG TGGAAAATGG CCCCTTGGAC CCGGCCAGCT CCTCCGCCTG GCACCGTCTT 
CGCCCCACAG ATGGCTCCTC TGGGCAGAAC AGCAAAGCTG GGACTGGGAT GAGCAAGTCG 
GCCTCATTTG CCTTTGAGTT CCCCAAGGAC AGAAGCGGGA TTGAGACGTT CTCTCCTCCT 
CCGCCACCTC CAAAGTCACG GCACCTTTTA AAAATGAACA AGAGCAGCTC TGATTTGGAG 
AAGGTGAGCC AGGGCTCTGC CGAGAGCCTC AGCCCGTCCT TCAGGGGCGT CCACGTCAGC 
TTCACCACGG GCTCCACGGA CAGCCTGGCC TCTGACTCCA GGACCTGCAG CGATGGAGGT 
CCATCATCTG AGCCAGCTCA CTCGCCCACC AACAGCAGGA AGAAGCTCTT TGCCCCCGTC 
CCCTTCCCTT CAGGCTCCAC AGAGGATGTG TCCCCCGGCG ACCCCCTGCA GCCCCCGCCT 
CTTCCCCAGA AGAAGCTCGT GAGTCGGGCG GCCTCTTCCC CTGATGGCTT CTTCTGGACC 
CAGGGCTCCC CCAAACCCAG AACAGCAAGT CCCAAGCTGA ACCTCAGCCA CTCGGAGACT 
AACGTCTGTG CCCATGAGGA CTCCCACTTC AGCTACGCCT CAGGCCCTGG CGGCCGCCAC 
CAGCACGTCT TCTCCGCTTC CGAGCCGCTG GAGAAAACTT CCAAAGGCAG TGGCCACCGC 
GGCCCTGTGC TGGGGCTGGG GGGCAGCAAA GGCCTGCAGG GCAAAGGGGT CTCCTCCGCC 
TCGTCCTCTC AGCTGAGCGT GTCCAGCCAG GCCTCGGCGG GCAGCACTCA GCTGCAGCTC 
CACAGCCTCC TGAACAGCAT CAGCAGCAAA GAGGGCACCT ACGCCAAGCT GGGGGGGCTC 
TACGCCCAGT CCCTGGTCCG CCTCGTGGCC AGGTGCGAGG ACCTCTTCAT GGGTGGCCAG 
AAGAAGGAGC TGCACTTCAG CGAGAATAAC TGGTCCCTGT TCAAGCTGAC CTGTAACAAG 
CCCTGCTGTG ACTCGGGGGA TGCCATTTAC TATTGCGCCA CCTGCTCCGA GGACCCCGGC 
AGCACCTACG CCGTGAAGAT CTGCAAGACC CCCGAGCCCA AAGCAGCCTC CTACTGCAGC 
CCTTCAGTGC CCGTGCACTT CAATATCCAG CAGGACTGTG GCCACTTTGT CGCCTCCGTG 
CCCTCCAGCA TGCTCACCTC TCCCGACGCA CACAAGGACC CTCTGTCTGC CCTGCCCTCA 
CATCCCCCCG CCCAGGAGCA GGACTGCGTG GTGGTCATCA CTCGGGAGGT GCCCCACCAA 
ACCGCCTCGG ACTTTGTGCG GGACTTGGCC GCCAGCCACC AGTCTGAGCC GGAGGTTTAC 
GAGCGGCGGG TGTGCTTCCT GCTCCTGCAG CTCTGCAACG GGCTGGAGCA CCTGAAGGAG 
CATGGGATCA TCCACCGAGA CCTGTGCCTG GAGAACCTGC TGCTGGTGCA TTGCGCCCCC 
CAGGCCTCCC CCGAGCCTTC CACCGCTGCC TCCCTCCCTC CCACCACCAC ATCCCCTGCC 
CCTGCGGCTA CCCCCTCCTC TGGCCCCTCA GCCACCGTCC CTACCAGCGT CAACCCCACC 
CCTCCAGCCG CCCCCGCCTT TCAAGGAGGG TCCAGTGAGA AACACTTGCC CCGGCTTATC 
ATCAGCAACT TCTTGAAGGC CAAGCAGAAG CCAGGTGGTA CCACCAACCT GCAGCAGAAG 
AAGAGCCAGG CACGCCTGGC CCCCGAGATC GTGTCGGCCT CCCAGTACCG CAAGTTCGAC 
GAATTCCAAA CGGGCATCCT CATCTACGAG CTGCTCCACC AGCCCAACCC ATTCGAGGTG 
CGGGCGCAGC TCCGGGAGCA GGACTACCGG CAGGAGGACT TGCCCCCGCT GCCCGCGCTG 
TCCCTCTACT CGCCGGGTCT GCAGCGGCTC GCCCACCTGC TGCTGGAGGC CGACCCCATC 
AAGCGCATCC GCATCGGCGA GGCCAAGCGC GTGCTGCAGT GCCTGCTGTG GGGGCCCCGG 
CGCGAGCTGG TGGAGCCGCC GGGCGCCTCG GAGGAGGTGC TGTGCACCAC GCTCCACAAC 
TGGATCGACA TGAAGCGAGC CCTGATGATG ATGAAGTTCG CCGAGAAGGC GGTGGACCGC 
CGGCGCGGGG TGGAGCTGGA GGACTGGCTC TGCTGCCAGT ATCTGGCGTC GGCCGAGCCC 
ACAGCCCTCT TACAATCACT GAAGCTCCTA CAGCTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bos taurus (cattle)	LOC533821	XP_005226093.1
Canis lupus familiaris (dog)	LOC482885	XP_540000.3
Xenopus tropicalis (tropical clawed frog)	LOC100486686	XP_002936500.1
Mus musculus (house mouse)	D8Ertd82e	NP_766499.2
Rattus norvegicus (Norway rat)	Pragmin	NP_001100785.1
Homo sapiens (human)	SGK223	NP_001074295.1
	LOC706349	XP_001094718.1
Pan troglodytes (chimpanzee)	LOC472692	XP_001136236.1
Gallus gallus (chicken)	SGK223	XP_420588.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PRAG1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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