MLH1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MLH1
Entrez Gene ID	533652
Full Name	mutL homolog 1
General protein information	Preferred Names mutL homolog 1 Names DNA mismatch repair protein Mlh1 MutL protein homolog 1 mutL homolog 1, colon cancer, nonpolyposis type 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024982839.1	XP_024838607.1	DNA mismatch repair protein Mlh1 isoform X2
XM_024982840.1	XP_024838608.1	DNA mismatch repair protein Mlh1 isoform X2
XM_024982841.1	XP_024838609.1	DNA mismatch repair protein Mlh1 isoform X2
NM_001075994.2	NP_001069462.2	DNA mismatch repair protein Mlh1
XM_005222490.3	XP_005222547.1	DNA mismatch repair protein Mlh1 isoform X1
XM_005222490.4	XP_005222547.1	DNA mismatch repair protein Mlh1 isoform X1
XM_024982842.1	XP_024838610.1	DNA mismatch repair protein Mlh1 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mlh1	NP_112315.1
Drosophila melanogaster (fruit fly)	Mlh1	NP_477022.1
Magnaporthe oryzae (rice blast fungus)	MGG_07121	XP_003715311.1
Oryza sativa (rice)	Os01g0958900	NP_001045457.1
Xenopus tropicalis (tropical clawed frog)	mlh1	XP_002933455.2
Pan troglodytes (chimpanzee)	MLH1	XP_001170433.1
Canis lupus familiaris (dog)	MLH1	XP_534219.2
Danio rerio (zebrafish)	mlh1	NP_956953.1
Schizosaccharomyces pombe (fission yeast)	mlh1	NP_596199.1
Neurospora crassa	NCU08309	XP_962522.1
Gallus gallus (chicken)	MLH1	XP_418828.1
Saccharomyces cerevisiae (baker's yeast)	MLH1	NP_013890.1
Kluyveromyces lactis	KLLA0D09955g	XP_453504.1
Bos taurus (cattle)	MLH1	NP_001069462.2
Mus musculus (house mouse)	Mlh1	NP_081086.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012192	XP_320342.4
Homo sapiens (human)	MLH1	NP_000240.1
Eremothecium gossypii	AGOS_AFL199C	NP_985351.1
Arabidopsis thaliana (thale cress)	MLH1	NP_567345.2

Gene Ontology
Bibliography

Related articles in PubMed

Meiosis progression and donor age affect expression profile of DNA repair genes in bovine oocytes.
Bilotto S, Boni R, Russo GL, Lioi MB
Zygote (Cambridge, England)23(1)11-8(2015 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Meiosis progression and female age affect expression profile of DNA repair MLH1 gene in bovine oocytes.
Title: Meiosis progression and donor age affect expression profile of DNA repair genes in bovine oocytes.

The following MLH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40278	NM_001075994.2 Latest version!	Bos taurus mutL homolog 1 (MLH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa31336	XM_005222490.4 Latest version!	Bos taurus mutL homolog 1 (MLH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa31338	XM_024982840.1 Latest version!	Bos taurus mutL homolog 1 (MLH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa31338	XM_024982842.1 Latest version!	Bos taurus mutL homolog 1 (MLH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa31338	XM_024982839.1 Latest version!	Bos taurus mutL homolog 1 (MLH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa31338	XM_024982841.1 Latest version!	Bos taurus mutL homolog 1 (MLH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBa31336	XM_005222490.3	Bos taurus mutL homolog 1 (MLH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MLH1 ( NM_001075994.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069462.2 Bos taurus mutL homolog 1 (MLH1), mRNA.

Price & Availability↑

CloneID	OBa40278
Clone ID Related Accession (Same CDS sequence)	NM_001075994.2
Accession Version	NM_001075994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	DNA mismatch repair protein Mlh1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV868803.1, DV934760.1, EH126978.1 and DV934932.1. On Dec 23, 2009 this sequence version replaced NM_001075994.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCGCTTG TAGCGGGGGT TATCCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCAGGGGAAG TTATCCAGCG GCCGGCTAAT GCCATCAAGG AAATGATTGA GAACTGTTTA 
GATGCAAAAT CCACAAGTAT TCAAGTGGTC GTTAAAGAGG GAGGCCTGAA GTTGATTCAG 
ATTCAAGACA ATGGCACTGG GATCAGGAAA GAAGATCTTG AGATTGTGTG TGAGAGGTTC 
ACCACAAGTA AACTGCAGTC CTTTGAGGAT TTAGCTCATA TTTCTACCTA TGGCTTTCGG 
GGTGAGGCTC TGGCCAGCAT AAGCCACGTG GCTCACGTCA CCATTACAAC CAAAACGGCT 
GACGGGAAGT GTGCATACAG AGCTCATTAC TCAGATGGAA AGCTGAAAGC ACCTCCTAAA 
CCGTGTGCAG GCAACCAAGG GACCCAGATC ACGGTGGAAG ACCTTTTTTA CAACATATCC 
ACGAGGAGGA AAGCTTTAAA AAACCCAAGT GAAGAGTATG GAAAAATTTT GGAAGTTGTT 
GGCAGGTATG CAGTACACAA TTCGGGCATT GGTTTCTCAG TTAAAAAACA AGGTGAAACT 
GTAGCTGACG TCAGGACCCT GCCAAATGCC ACCACCGTGG ACAACATTCG CTCCATCTTT 
GGAAATGCTG TTAGCCGAGA ATTGATAGAA GTTGAATGTG AGGATAAAAC CCTTGCCTTC 
AAAATGAATG GCTACATATC CAATGCAAAC TATTCAGTGA AGAAGTGCAT CTTCTTACTC 
TTCATCAACC ATCGTCTGGT AGAGTCAGCT TCCTTAAGAA AAGCCATAGA AACGGTGTAT 
GCAGCGTATT TGCCCAAAAG CACACACCCG TTCCTGTACC TCAGTCTAGA AATAAGCCCC 
CAGAATGTGG ATGTCAACGT GCACCCCACG AAGCACGAGG TTCACTTCCT GCATGAGGAC 
AGCATCCTGG AACGGCTGCA GCAGCACATC GAGAGCAGGC TCCTGGGCTC CAACGCCTCC 
AGGACCTACT TCACCCAGAC TTTACTGCCA GGTCTTCCTG GCCCCTCTGG GGAAGCAGTT 
AAATCCACAG CGAGTGTGAC ATCCTCATCC ACTGCTGGAA GTGGTGACAG AGTGTATGCC 
CACCAGATGG TCCGCACAGA CTGCCGGGAA CAGAAGCTCG ATGCCTTCCT GCAGCCTGTG 
AGCAAGGCCC TGTCCAGTCA GCCCCAGGCT GTCGTCCCAG AGCACAGGAC AGATGCTTCT 
AGCAGTGGGA CCAGGCAGCA GGACGAGGAG ATGCTTGAAC TCCCAGCTCC TGCTGCAGTG 
GCTGCGAAGA GTCAGGCCTT AGAGGACGAT GCAACAATGA GGGCTGCAGA CCTGGCCGAG 
AAGAGAGGGC CCTCTTCCAG CCCCGAGAAC CCCAGAAAGA GACCTCGGGA AGACTCTGAT 
GTGGAAATGG TGGAGGATGC ATCCCGAAAG GAGATGACAG CCGCTTGTAC CCCCCGGAGA 
AGGATCATCA ACCTTACCAG TGTTCTGAGT CTCCAGGAGG AGATCAACGA GCGGGGCCAT 
GAGACTCTCC GGGAGATGCT GCATAACCAC TCCTTTGTGG GCTGCGTGAA TCCTCAGTGG 
GCCTTGGCAC AGCATCAGAC CAAGTTATAC CTTCTCAACA CCACCAGACT TAGTGAAGAA 
CTGTTCTACC AGATTCTCGT TTATGATTTT GCCAATTTTG GTGTTCTGAG GTTATCGGAG 
CCAGCACCAC TCTTTGACCT GGCCATGCTC GCCTTAGACA GTCCCGAGAG TGGCTGGACA 
GAGGAGGATG GTCCCAAGGA AGGACTTGCT GAGTACATTG TTGAGTTTCT GAAAAAGAAG 
GCTGAGATGC TTGCAGACTA CTTCTCTCTG GAGATTGATG AGGAAGGGAA CCTGGTTGGA 
TTACCCCTTC TGATCGACAA CTATGTGCCC CCGCTGGAGG GTCTGCCTAT CTTCATCCTC 
CGACTGGCCA CAGAGGTGAA CTGGGATGAA GAAAAGGAAT GTTTTGAAAG CCTCAGTAAA 
GAATGTGCGA TGTTTTACTC CATCCGGAAG CAGTATGTGT CTGCAGAGTC AACCCTTTCA 
GGCCAGCAGA GTGAAGTGCC TGGCTCCACT GCAAACCCCT GGAAGTGGAC TGTGGAACAC 
GTCATTTATA AAGCCTTCCG CTCCCACCTG CTGCCTCCAA AACACTTCAC AGAAGATGGG 
AACATCCTGC AGCTTGCTAA CCTGCCTGAT CTCTACAAAG TCTTCGAGAG GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069462.2
CDS	85..2361
Translation	MSLVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVVVKEGGLKLIQIQDNGTGIRKEDLEIVCERFTTSKLQSFEDLAHISTYGFRGEALASISHVAHVTITTKTADGKCAYRAHYSDGKLKAPPKPCAGNQGTQITVEDLFYNISTRRKALKNPSEEYGKILEVVGRYAVHNSGIGFSVKKQGETVADVRTLPNATTVDNIRSIFGNAVSRELIEVECEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESASLRKAIETVYAAYLPKSTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEDSILERLQQHIESRLLGSNASRTYFTQTLLPGLPGPSGEAVKSTASVTSSSTAGSGDRVYAHQMVRTDCREQKLDAFLQPVSKALSSQPQAVVPEHRTDASSSGTRQQDEEMLELPAPAAVAAKSQALEDDATMRAADLAEKRGPSSSPENPRKRPREDSDVEMVEDASRKEMTAACTPRRRIINLTSVLSLQEEINERGHETLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTRLSEELFYQILVYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLVGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYVSAESTLSGQQSEVPGSTANPWKWTVEHVIYKAFRSHLLPPKHFTEDGNILQLANLPDLYKVFERC

Target ORF information:

RefSeq Version	NM_001075994.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mutL homolog 1 (MLH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075994.2

ORF Insert Sequence:

ATGTCGCTTG TAGCGGGGGT TATCCGGCGG CTGGACGAGA CAGTGGTGAA CCGCATCGCG 
GCAGGGGAAG TTATCCAGCG GCCGGCTAAT GCCATCAAGG AAATGATTGA GAACTGTTTA 
GATGCAAAAT CCACAAGTAT TCAAGTGGTC GTTAAAGAGG GAGGCCTGAA GTTGATTCAG 
ATTCAAGACA ATGGCACTGG GATCAGGAAA GAAGATCTTG AGATTGTGTG TGAGAGGTTC 
ACCACAAGTA AACTGCAGTC CTTTGAGGAT TTAGCTCATA TTTCTACCTA TGGCTTTCGG 
GGTGAGGCTC TGGCCAGCAT AAGCCACGTG GCTCACGTCA CCATTACAAC CAAAACGGCT 
GACGGGAAGT GTGCATACAG AGCTCATTAC TCAGATGGAA AGCTGAAAGC ACCTCCTAAA 
CCGTGTGCAG GCAACCAAGG GACCCAGATC ACGGTGGAAG ACCTTTTTTA CAACATATCC 
ACGAGGAGGA AAGCTTTAAA AAACCCAAGT GAAGAGTATG GAAAAATTTT GGAAGTTGTT 
GGCAGGTATG CAGTACACAA TTCGGGCATT GGTTTCTCAG TTAAAAAACA AGGTGAAACT 
GTAGCTGACG TCAGGACCCT GCCAAATGCC ACCACCGTGG ACAACATTCG CTCCATCTTT 
GGAAATGCTG TTAGCCGAGA ATTGATAGAA GTTGAATGTG AGGATAAAAC CCTTGCCTTC 
AAAATGAATG GCTACATATC CAATGCAAAC TATTCAGTGA AGAAGTGCAT CTTCTTACTC 
TTCATCAACC ATCGTCTGGT AGAGTCAGCT TCCTTAAGAA AAGCCATAGA AACGGTGTAT 
GCAGCGTATT TGCCCAAAAG CACACACCCG TTCCTGTACC TCAGTCTAGA AATAAGCCCC 
CAGAATGTGG ATGTCAACGT GCACCCCACG AAGCACGAGG TTCACTTCCT GCATGAGGAC 
AGCATCCTGG AACGGCTGCA GCAGCACATC GAGAGCAGGC TCCTGGGCTC CAACGCCTCC 
AGGACCTACT TCACCCAGAC TTTACTGCCA GGTCTTCCTG GCCCCTCTGG GGAAGCAGTT 
AAATCCACAG CGAGTGTGAC ATCCTCATCC ACTGCTGGAA GTGGTGACAG AGTGTATGCC 
CACCAGATGG TCCGCACAGA CTGCCGGGAA CAGAAGCTCG ATGCCTTCCT GCAGCCTGTG 
AGCAAGGCCC TGTCCAGTCA GCCCCAGGCT GTCGTCCCAG AGCACAGGAC AGATGCTTCT 
AGCAGTGGGA CCAGGCAGCA GGACGAGGAG ATGCTTGAAC TCCCAGCTCC TGCTGCAGTG 
GCTGCGAAGA GTCAGGCCTT AGAGGACGAT GCAACAATGA GGGCTGCAGA CCTGGCCGAG 
AAGAGAGGGC CCTCTTCCAG CCCCGAGAAC CCCAGAAAGA GACCTCGGGA AGACTCTGAT 
GTGGAAATGG TGGAGGATGC ATCCCGAAAG GAGATGACAG CCGCTTGTAC CCCCCGGAGA 
AGGATCATCA ACCTTACCAG TGTTCTGAGT CTCCAGGAGG AGATCAACGA GCGGGGCCAT 
GAGACTCTCC GGGAGATGCT GCATAACCAC TCCTTTGTGG GCTGCGTGAA TCCTCAGTGG 
GCCTTGGCAC AGCATCAGAC CAAGTTATAC CTTCTCAACA CCACCAGACT TAGTGAAGAA 
CTGTTCTACC AGATTCTCGT TTATGATTTT GCCAATTTTG GTGTTCTGAG GTTATCGGAG 
CCAGCACCAC TCTTTGACCT GGCCATGCTC GCCTTAGACA GTCCCGAGAG TGGCTGGACA 
GAGGAGGATG GTCCCAAGGA AGGACTTGCT GAGTACATTG TTGAGTTTCT GAAAAAGAAG 
GCTGAGATGC TTGCAGACTA CTTCTCTCTG GAGATTGATG AGGAAGGGAA CCTGGTTGGA 
TTACCCCTTC TGATCGACAA CTATGTGCCC CCGCTGGAGG GTCTGCCTAT CTTCATCCTC 
CGACTGGCCA CAGAGGTGAA CTGGGATGAA GAAAAGGAAT GTTTTGAAAG CCTCAGTAAA 
GAATGTGCGA TGTTTTACTC CATCCGGAAG CAGTATGTGT CTGCAGAGTC AACCCTTTCA 
GGCCAGCAGA GTGAAGTGCC TGGCTCCACT GCAAACCCCT GGAAGTGGAC TGTGGAACAC 
GTCATTTATA AAGCCTTCCG CTCCCACCTG CTGCCTCCAA AACACTTCAC AGAAGATGGG 
AACATCCTGC AGCTTGCTAA CCTGCCTGAT CTCTACAAAG TCTTCGAGAG GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_005222490.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222547.1 Bos taurus mutL homolog 1 (MLH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31336
Clone ID Related Accession (Same CDS sequence)	XM_005222490.3 , XM_005222490.4
Accession Version	XM_005222490.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA mismatch repair protein Mlh1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104540.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTATC AGTTTCTTGC TTATTCCATA AGTCGTCCAT CGACATTCAA CGATGCAAAA 
TCCACAAGTA TTCAAGTGGT CGTTAAAGAG GGAGGCCTGA AGTTGATTCA GATTCAAGAC 
AATGGCACTG GGATCAGGAA AGAAGATCTT GAGATTGTGT GTGAGAGGTT CACCACAAGT 
AAACTGCAGT CCTTTGAGGA TTTAGCTCAT ATTTCTACCT ATGGCTTTCG GGGTGAGGCT 
CTGGCCAGCA TAAGCCACGT GGCTCACGTC ACCATTACAA CCAAAACGGC TGACGGGAAG 
TGTGCATACA GAGCTCATTA CTCAGATGGA AAGCTGAAAG CACCTCCTAA ACCGTGTGCA 
GGCAACCAAG GGACCCAGAT CACGGTGGAA GACCTTTTTT ACAACATATC CACGAGGAGG 
AAAGCTTTAA AAAACCCAAG TGAAGAGTAT GGAAAAATTT TGGAAGTTGT TGGCAGGTAT 
GCAGTACACA ATTCGGGCAT TGGTTTCTCA GTTAAAAAAC AAGGTGAAAC TGTAGCTGAC 
GTCAGGACCC TGCCAAATGC CACCACCGTG GACAACATTC GCTCCATCTT TGGAAATGCT 
GTTAGCCGAG AATTGATAGA AGTTGAATGT GAGGATAAAA CCCTTGCCTT CAAAATGAAT 
GGCTACATAT CCAATGCAAA CTATTCAGTG AAGAAGTGCA TCTTCTTACT CTTCATCAAC 
CATCGTCTGG TAGAGTCAGC TTCCTTAAGA AAAGCCATAG AAACGGTGTA TGCAGCGTAT 
TTGCCCAAAA GCACACACCC GTTCCTGTAC CTCAGTCTAG AAATAAGCCC CCAGAATGTG 
GATGTCAACG TGCACCCCAC GAAGCACGAG GTTCACTTCC TGCATGAGGA CAGCATCCTG 
GAACGGCTGC AGCAGCACAT CGAGAGCAGG CTCCTGGGCT CCAACGCCTC CAGGACCTAC 
TTCACCCAGA CTTTACTGCC AGGTCTTCCT GGCCCCTCTG GGGAAGCAGT TAAATCCACA 
GCGAGTGTGA CATCCTCATC CACTGCTGGA AGTGGTGACA GAGTGTATGC CCACCAGATG 
GTCCGCACAG ACTGCCGGGA ACAGAAGCTC GATGCCTTCC TGCAGCCTGT GAGCAAGGCC 
CTGTCCAGTC AGCCCCAGGC TGTCGTCCCA GAGCACAGGA CAGATGCTTC TAGCAGTGGG 
ACCAGGCAGC AGGACGAGGA GATGCTTGAA CTCCCAGCTC CTGCTGCAGT GGCTGCGAAG 
AGTCAGGCCT TAGAGGACGA TGCAACAATG AGGGCTGCAG ACCTGGCCGA GAAGAGAGGG 
CCCTCTTCCA GCCCCGAGAA CCCCAGAAAG AGACCTCGGG AAGACTCTGA TGTGGAAATG 
GTGGAGGATG CATCCCGAAA GGAGATGACA GCCGCTTGTA CCCCCCGGAG AAGGATCATC 
AACCTTACCA GTGTTCTGAG TCTCCAGGAG GAGATCAACG AGCGGGGCCA TGAGACTCTC 
CGGGAGATGC TGCATAACCA CTCCTTTGTG GGCTGCGTGA ATCCTCAGTG GGCCTTGGCA 
CAGCATCAGA CCAAGTTATA CCTTCTCAAC ACCACCAGAC TTAGTGAAGA ACTGTTCTAC 
CAGATTCTCG TTTATGATTT TGCCAATTTT GGTGTTCTGA GGTTATCGGA GCCAGCACCA 
CTCTTTGACC TGGCCATGCT CGCCTTAGAC AGTCCCGAGA GTGGCTGGAC AGAGGAGGAT 
GGTCCCAAGG AAGGACTTGC TGAGTACATT GTTGAGTTTC TGAAAAAGAA GGCTGAGATG 
CTTGCAGACT ACTTCTCTCT GGAGATTGAT GAGGAAGGGA ACCTGGTTGG ATTACCCCTT 
CTGATCGACA ACTATGTGCC CCCGCTGGAG GGTCTGCCTA TCTTCATCCT CCGACTGGCC 
ACAGAGGTGA ACTGGGATGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGTGCG 
ATGTTTTACT CCATCCGGAA GCAGTATGTG TCTGCAGAGT CAACCCTTTC AGGCCAGCAG 
AGTGAAGTGC CTGGCTCCAC TGCAAACCCC TGGAAGTGGA CTGTGGAACA CGTCATTTAT 
AAAGCCTTCC GCTCCCACCT GCTGCCTCCA AAACACTTCA CAGAAGATGG GAACATCCTG 
CAGCTTGCTA ACCTGCCTGA TCTCTACAAA GTCTTCGAGA GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222547.1
CDS	441..2648
Translation	MGYQFLAYSISRPSTFNDAKSTSIQVVVKEGGLKLIQIQDNGTGIRKEDLEIVCERFTTSKLQSFEDLAHISTYGFRGEALASISHVAHVTITTKTADGKCAYRAHYSDGKLKAPPKPCAGNQGTQITVEDLFYNISTRRKALKNPSEEYGKILEVVGRYAVHNSGIGFSVKKQGETVADVRTLPNATTVDNIRSIFGNAVSRELIEVECEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESASLRKAIETVYAAYLPKSTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEDSILERLQQHIESRLLGSNASRTYFTQTLLPGLPGPSGEAVKSTASVTSSSTAGSGDRVYAHQMVRTDCREQKLDAFLQPVSKALSSQPQAVVPEHRTDASSSGTRQQDEEMLELPAPAAVAAKSQALEDDATMRAADLAEKRGPSSSPENPRKRPREDSDVEMVEDASRKEMTAACTPRRRIINLTSVLSLQEEINERGHETLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTRLSEELFYQILVYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLVGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYVSAESTLSGQQSEVPGSTANPWKWTVEHVIYKAFRSHLLPPKHFTEDGNILQLANLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_005222490.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mutL homolog 1 (MLH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222490.3

ORF Insert Sequence:

ATGGGTTATC AGTTTCTTGC TTATTCCATA AGTCGTCCAT CGACATTCAA CGATGCAAAA 
TCCACAAGTA TTCAAGTGGT CGTTAAAGAG GGAGGCCTGA AGTTGATTCA GATTCAAGAC 
AATGGCACTG GGATCAGGAA AGAAGATCTT GAGATTGTGT GTGAGAGGTT CACCACAAGT 
AAACTGCAGT CCTTTGAGGA TTTAGCTCAT ATTTCTACCT ATGGCTTTCG GGGTGAGGCT 
CTGGCCAGCA TAAGCCACGT GGCTCACGTC ACCATTACAA CCAAAACGGC TGACGGGAAG 
TGTGCATACA GAGCTCATTA CTCAGATGGA AAGCTGAAAG CACCTCCTAA ACCGTGTGCA 
GGCAACCAAG GGACCCAGAT CACGGTGGAA GACCTTTTTT ACAACATATC CACGAGGAGG 
AAAGCTTTAA AAAACCCAAG TGAAGAGTAT GGAAAAATTT TGGAAGTTGT TGGCAGGTAT 
GCAGTACACA ATTCGGGCAT TGGTTTCTCA GTTAAAAAAC AAGGTGAAAC TGTAGCTGAC 
GTCAGGACCC TGCCAAATGC CACCACCGTG GACAACATTC GCTCCATCTT TGGAAATGCT 
GTTAGCCGAG AATTGATAGA AGTTGAATGT GAGGATAAAA CCCTTGCCTT CAAAATGAAT 
GGCTACATAT CCAATGCAAA CTATTCAGTG AAGAAGTGCA TCTTCTTACT CTTCATCAAC 
CATCGTCTGG TAGAGTCAGC TTCCTTAAGA AAAGCCATAG AAACGGTGTA TGCAGCGTAT 
TTGCCCAAAA GCACACACCC GTTCCTGTAC CTCAGTCTAG AAATAAGCCC CCAGAATGTG 
GATGTCAACG TGCACCCCAC GAAGCACGAG GTTCACTTCC TGCATGAGGA CAGCATCCTG 
GAACGGCTGC AGCAGCACAT CGAGAGCAGG CTCCTGGGCT CCAACGCCTC CAGGACCTAC 
TTCACCCAGA CTTTACTGCC AGGTCTTCCT GGCCCCTCTG GGGAAGCAGT TAAATCCACA 
GCGAGTGTGA CATCCTCATC CACTGCTGGA AGTGGTGACA GAGTGTATGC CCACCAGATG 
GTCCGCACAG ACTGCCGGGA ACAGAAGCTC GATGCCTTCC TGCAGCCTGT GAGCAAGGCC 
CTGTCCAGTC AGCCCCAGGC TGTCGTCCCA GAGCACAGGA CAGATGCTTC TAGCAGTGGG 
ACCAGGCAGC AGGACGAGGA GATGCTTGAA CTCCCAGCTC CTGCTGCAGT GGCTGCGAAG 
AGTCAGGCCT TAGAGGACGA TGCAACAATG AGGGCTGCAG ACCTGGCCGA GAAGAGAGGG 
CCCTCTTCCA GCCCCGAGAA CCCCAGAAAG AGACCTCGGG AAGACTCTGA TGTGGAAATG 
GTGGAGGATG CATCCCGAAA GGAGATGACA GCCGCTTGTA CCCCCCGGAG AAGGATCATC 
AACCTTACCA GTGTTCTGAG TCTCCAGGAG GAGATCAACG AGCGGGGCCA TGAGACTCTC 
CGGGAGATGC TGCATAACCA CTCCTTTGTG GGCTGCGTGA ATCCTCAGTG GGCCTTGGCA 
CAGCATCAGA CCAAGTTATA CCTTCTCAAC ACCACCAGAC TTAGTGAAGA ACTGTTCTAC 
CAGATTCTCG TTTATGATTT TGCCAATTTT GGTGTTCTGA GGTTATCGGA GCCAGCACCA 
CTCTTTGACC TGGCCATGCT CGCCTTAGAC AGTCCCGAGA GTGGCTGGAC AGAGGAGGAT 
GGTCCCAAGG AAGGACTTGC TGAGTACATT GTTGAGTTTC TGAAAAAGAA GGCTGAGATG 
CTTGCAGACT ACTTCTCTCT GGAGATTGAT GAGGAAGGGA ACCTGGTTGG ATTACCCCTT 
CTGATCGACA ACTATGTGCC CCCGCTGGAG GGTCTGCCTA TCTTCATCCT CCGACTGGCC 
ACAGAGGTGA ACTGGGATGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGTGCG 
ATGTTTTACT CCATCCGGAA GCAGTATGTG TCTGCAGAGT CAACCCTTTC AGGCCAGCAG 
AGTGAAGTGC CTGGCTCCAC TGCAAACCCC TGGAAGTGGA CTGTGGAACA CGTCATTTAT 
AAAGCCTTCC GCTCCCACCT GCTGCCTCCA AAACACTTCA CAGAAGATGG GAACATCCTG 
CAGCTTGCTA ACCTGCCTGA TCTCTACAAA GTCTTCGAGA GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_005222490.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222547.1 Bos taurus mutL homolog 1 (MLH1), transcript variant X1, mRNA.

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CloneID	OBa31336
Clone ID Related Accession (Same CDS sequence)	XM_005222490.3 , XM_005222490.4
Accession Version	XM_005222490.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA mismatch repair protein Mlh1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222490.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTATC AGTTTCTTGC TTATTCCATA AGTCGTCCAT CGACATTCAA CGATGCAAAA 
TCCACAAGTA TTCAAGTGGT CGTTAAAGAG GGAGGCCTGA AGTTGATTCA GATTCAAGAC 
AATGGCACTG GGATCAGGAA AGAAGATCTT GAGATTGTGT GTGAGAGGTT CACCACAAGT 
AAACTGCAGT CCTTTGAGGA TTTAGCTCAT ATTTCTACCT ATGGCTTTCG GGGTGAGGCT 
CTGGCCAGCA TAAGCCACGT GGCTCACGTC ACCATTACAA CCAAAACGGC TGACGGGAAG 
TGTGCATACA GAGCTCATTA CTCAGATGGA AAGCTGAAAG CACCTCCTAA ACCGTGTGCA 
GGCAACCAAG GGACCCAGAT CACGGTGGAA GACCTTTTTT ACAACATATC CACGAGGAGG 
AAAGCTTTAA AAAACCCAAG TGAAGAGTAT GGAAAAATTT TGGAAGTTGT TGGCAGGTAT 
GCAGTACACA ATTCGGGCAT TGGTTTCTCA GTTAAAAAAC AAGGTGAAAC TGTAGCTGAC 
GTCAGGACCC TGCCAAATGC CACCACCGTG GACAACATTC GCTCCATCTT TGGAAATGCT 
GTTAGCCGAG AATTGATAGA AGTTGAATGT GAGGATAAAA CCCTTGCCTT CAAAATGAAT 
GGCTACATAT CCAATGCAAA CTATTCAGTG AAGAAGTGCA TCTTCTTACT CTTCATCAAC 
CATCGTCTGG TAGAGTCAGC TTCCTTAAGA AAAGCCATAG AAACGGTGTA TGCAGCGTAT 
TTGCCCAAAA GCACACACCC GTTCCTGTAC CTCAGTCTAG AAATAAGCCC CCAGAATGTG 
GATGTCAACG TGCACCCCAC GAAGCACGAG GTTCACTTCC TGCATGAGGA CAGCATCCTG 
GAACGGCTGC AGCAGCACAT CGAGAGCAGG CTCCTGGGCT CCAACGCCTC CAGGACCTAC 
TTCACCCAGA CTTTACTGCC AGGTCTTCCT GGCCCCTCTG GGGAAGCAGT TAAATCCACA 
GCGAGTGTGA CATCCTCATC CACTGCTGGA AGTGGTGACA GAGTGTATGC CCACCAGATG 
GTCCGCACAG ACTGCCGGGA ACAGAAGCTC GATGCCTTCC TGCAGCCTGT GAGCAAGGCC 
CTGTCCAGTC AGCCCCAGGC TGTCGTCCCA GAGCACAGGA CAGATGCTTC TAGCAGTGGG 
ACCAGGCAGC AGGACGAGGA GATGCTTGAA CTCCCAGCTC CTGCTGCAGT GGCTGCGAAG 
AGTCAGGCCT TAGAGGACGA TGCAACAATG AGGGCTGCAG ACCTGGCCGA GAAGAGAGGG 
CCCTCTTCCA GCCCCGAGAA CCCCAGAAAG AGACCTCGGG AAGACTCTGA TGTGGAAATG 
GTGGAGGATG CATCCCGAAA GGAGATGACA GCCGCTTGTA CCCCCCGGAG AAGGATCATC 
AACCTTACCA GTGTTCTGAG TCTCCAGGAG GAGATCAACG AGCGGGGCCA TGAGACTCTC 
CGGGAGATGC TGCATAACCA CTCCTTTGTG GGCTGCGTGA ATCCTCAGTG GGCCTTGGCA 
CAGCATCAGA CCAAGTTATA CCTTCTCAAC ACCACCAGAC TTAGTGAAGA ACTGTTCTAC 
CAGATTCTCG TTTATGATTT TGCCAATTTT GGTGTTCTGA GGTTATCGGA GCCAGCACCA 
CTCTTTGACC TGGCCATGCT CGCCTTAGAC AGTCCCGAGA GTGGCTGGAC AGAGGAGGAT 
GGTCCCAAGG AAGGACTTGC TGAGTACATT GTTGAGTTTC TGAAAAAGAA GGCTGAGATG 
CTTGCAGACT ACTTCTCTCT GGAGATTGAT GAGGAAGGGA ACCTGGTTGG ATTACCCCTT 
CTGATCGACA ACTATGTGCC CCCGCTGGAG GGTCTGCCTA TCTTCATCCT CCGACTGGCC 
ACAGAGGTGA ACTGGGATGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGTGCG 
ATGTTTTACT CCATCCGGAA GCAGTATGTG TCTGCAGAGT CAACCCTTTC AGGCCAGCAG 
AGTGAAGTGC CTGGCTCCAC TGCAAACCCC TGGAAGTGGA CTGTGGAACA CGTCATTTAT 
AAAGCCTTCC GCTCCCACCT GCTGCCTCCA AAACACTTCA CAGAAGATGG GAACATCCTG 
CAGCTTGCTA ACCTGCCTGA TCTCTACAAA GTCTTCGAGA GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222547.1
CDS	770..2977
Translation	MGYQFLAYSISRPSTFNDAKSTSIQVVVKEGGLKLIQIQDNGTGIRKEDLEIVCERFTTSKLQSFEDLAHISTYGFRGEALASISHVAHVTITTKTADGKCAYRAHYSDGKLKAPPKPCAGNQGTQITVEDLFYNISTRRKALKNPSEEYGKILEVVGRYAVHNSGIGFSVKKQGETVADVRTLPNATTVDNIRSIFGNAVSRELIEVECEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESASLRKAIETVYAAYLPKSTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEDSILERLQQHIESRLLGSNASRTYFTQTLLPGLPGPSGEAVKSTASVTSSSTAGSGDRVYAHQMVRTDCREQKLDAFLQPVSKALSSQPQAVVPEHRTDASSSGTRQQDEEMLELPAPAAVAAKSQALEDDATMRAADLAEKRGPSSSPENPRKRPREDSDVEMVEDASRKEMTAACTPRRRIINLTSVLSLQEEINERGHETLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTRLSEELFYQILVYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLVGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYVSAESTLSGQQSEVPGSTANPWKWTVEHVIYKAFRSHLLPPKHFTEDGNILQLANLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_005222490.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mutL homolog 1 (MLH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222490.4

ORF Insert Sequence:

ATGGGTTATC AGTTTCTTGC TTATTCCATA AGTCGTCCAT CGACATTCAA CGATGCAAAA 
TCCACAAGTA TTCAAGTGGT CGTTAAAGAG GGAGGCCTGA AGTTGATTCA GATTCAAGAC 
AATGGCACTG GGATCAGGAA AGAAGATCTT GAGATTGTGT GTGAGAGGTT CACCACAAGT 
AAACTGCAGT CCTTTGAGGA TTTAGCTCAT ATTTCTACCT ATGGCTTTCG GGGTGAGGCT 
CTGGCCAGCA TAAGCCACGT GGCTCACGTC ACCATTACAA CCAAAACGGC TGACGGGAAG 
TGTGCATACA GAGCTCATTA CTCAGATGGA AAGCTGAAAG CACCTCCTAA ACCGTGTGCA 
GGCAACCAAG GGACCCAGAT CACGGTGGAA GACCTTTTTT ACAACATATC CACGAGGAGG 
AAAGCTTTAA AAAACCCAAG TGAAGAGTAT GGAAAAATTT TGGAAGTTGT TGGCAGGTAT 
GCAGTACACA ATTCGGGCAT TGGTTTCTCA GTTAAAAAAC AAGGTGAAAC TGTAGCTGAC 
GTCAGGACCC TGCCAAATGC CACCACCGTG GACAACATTC GCTCCATCTT TGGAAATGCT 
GTTAGCCGAG AATTGATAGA AGTTGAATGT GAGGATAAAA CCCTTGCCTT CAAAATGAAT 
GGCTACATAT CCAATGCAAA CTATTCAGTG AAGAAGTGCA TCTTCTTACT CTTCATCAAC 
CATCGTCTGG TAGAGTCAGC TTCCTTAAGA AAAGCCATAG AAACGGTGTA TGCAGCGTAT 
TTGCCCAAAA GCACACACCC GTTCCTGTAC CTCAGTCTAG AAATAAGCCC CCAGAATGTG 
GATGTCAACG TGCACCCCAC GAAGCACGAG GTTCACTTCC TGCATGAGGA CAGCATCCTG 
GAACGGCTGC AGCAGCACAT CGAGAGCAGG CTCCTGGGCT CCAACGCCTC CAGGACCTAC 
TTCACCCAGA CTTTACTGCC AGGTCTTCCT GGCCCCTCTG GGGAAGCAGT TAAATCCACA 
GCGAGTGTGA CATCCTCATC CACTGCTGGA AGTGGTGACA GAGTGTATGC CCACCAGATG 
GTCCGCACAG ACTGCCGGGA ACAGAAGCTC GATGCCTTCC TGCAGCCTGT GAGCAAGGCC 
CTGTCCAGTC AGCCCCAGGC TGTCGTCCCA GAGCACAGGA CAGATGCTTC TAGCAGTGGG 
ACCAGGCAGC AGGACGAGGA GATGCTTGAA CTCCCAGCTC CTGCTGCAGT GGCTGCGAAG 
AGTCAGGCCT TAGAGGACGA TGCAACAATG AGGGCTGCAG ACCTGGCCGA GAAGAGAGGG 
CCCTCTTCCA GCCCCGAGAA CCCCAGAAAG AGACCTCGGG AAGACTCTGA TGTGGAAATG 
GTGGAGGATG CATCCCGAAA GGAGATGACA GCCGCTTGTA CCCCCCGGAG AAGGATCATC 
AACCTTACCA GTGTTCTGAG TCTCCAGGAG GAGATCAACG AGCGGGGCCA TGAGACTCTC 
CGGGAGATGC TGCATAACCA CTCCTTTGTG GGCTGCGTGA ATCCTCAGTG GGCCTTGGCA 
CAGCATCAGA CCAAGTTATA CCTTCTCAAC ACCACCAGAC TTAGTGAAGA ACTGTTCTAC 
CAGATTCTCG TTTATGATTT TGCCAATTTT GGTGTTCTGA GGTTATCGGA GCCAGCACCA 
CTCTTTGACC TGGCCATGCT CGCCTTAGAC AGTCCCGAGA GTGGCTGGAC AGAGGAGGAT 
GGTCCCAAGG AAGGACTTGC TGAGTACATT GTTGAGTTTC TGAAAAAGAA GGCTGAGATG 
CTTGCAGACT ACTTCTCTCT GGAGATTGAT GAGGAAGGGA ACCTGGTTGG ATTACCCCTT 
CTGATCGACA ACTATGTGCC CCCGCTGGAG GGTCTGCCTA TCTTCATCCT CCGACTGGCC 
ACAGAGGTGA ACTGGGATGA AGAAAAGGAA TGTTTTGAAA GCCTCAGTAA AGAATGTGCG 
ATGTTTTACT CCATCCGGAA GCAGTATGTG TCTGCAGAGT CAACCCTTTC AGGCCAGCAG 
AGTGAAGTGC CTGGCTCCAC TGCAAACCCC TGGAAGTGGA CTGTGGAACA CGTCATTTAT 
AAAGCCTTCC GCTCCCACCT GCTGCCTCCA AAACACTTCA CAGAAGATGG GAACATCCTG 
CAGCTTGCTA ACCTGCCTGA TCTCTACAAA GTCTTCGAGA GGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_024982839.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838607.1 Bos taurus mutL homolog 1 (MLH1), transcript variant X2, mRNA.

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CloneID	OBa31338
Clone ID Related Accession (Same CDS sequence)	XM_024982839.1 , XM_024982840.1 , XM_024982841.1 , XM_024982842.1
Accession Version	XM_024982839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA mismatch repair protein Mlh1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838607.1
CDS	1429..2982
Translation	MNGYISNANYSVKKCIFLLFINHRLVESASLRKAIETVYAAYLPKSTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEDSILERLQQHIESRLLGSNASRTYFTQTLLPGLPGPSGEAVKSTASVTSSSTAGSGDRVYAHQMVRTDCREQKLDAFLQPVSKALSSQPQAVVPEHRTDASSSGTRQQDEEMLELPAPAAVAAKSQALEDDATMRAADLAEKRGPSSSPENPRKRPREDSDVEMVEDASRKEMTAACTPRRRIINLTSVLSLQEEINERGHETLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTRLSEELFYQILVYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLVGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYVSAESTLSGQQSEVPGSTANPWKWTVEHVIYKAFRSHLLPPKHFTEDGNILQLANLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_024982839.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mutL homolog 1 (MLH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982839.1

ORF Insert Sequence:

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_024982840.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838608.1 Bos taurus mutL homolog 1 (MLH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa31338
Clone ID Related Accession (Same CDS sequence)	XM_024982839.1 , XM_024982840.1 , XM_024982841.1 , XM_024982842.1
Accession Version	XM_024982840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA mismatch repair protein Mlh1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838608.1
CDS	743..2296
Translation	MNGYISNANYSVKKCIFLLFINHRLVESASLRKAIETVYAAYLPKSTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEDSILERLQQHIESRLLGSNASRTYFTQTLLPGLPGPSGEAVKSTASVTSSSTAGSGDRVYAHQMVRTDCREQKLDAFLQPVSKALSSQPQAVVPEHRTDASSSGTRQQDEEMLELPAPAAVAAKSQALEDDATMRAADLAEKRGPSSSPENPRKRPREDSDVEMVEDASRKEMTAACTPRRRIINLTSVLSLQEEINERGHETLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTRLSEELFYQILVYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLVGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYVSAESTLSGQQSEVPGSTANPWKWTVEHVIYKAFRSHLLPPKHFTEDGNILQLANLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_024982840.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mutL homolog 1 (MLH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982840.1

ORF Insert Sequence:

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_024982841.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838609.1 Bos taurus mutL homolog 1 (MLH1), transcript variant X4, mRNA.

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CloneID	OBa31338
Clone ID Related Accession (Same CDS sequence)	XM_024982839.1 , XM_024982840.1 , XM_024982841.1 , XM_024982842.1
Accession Version	XM_024982841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA mismatch repair protein Mlh1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838609.1
CDS	1038..2591
Translation	MNGYISNANYSVKKCIFLLFINHRLVESASLRKAIETVYAAYLPKSTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEDSILERLQQHIESRLLGSNASRTYFTQTLLPGLPGPSGEAVKSTASVTSSSTAGSGDRVYAHQMVRTDCREQKLDAFLQPVSKALSSQPQAVVPEHRTDASSSGTRQQDEEMLELPAPAAVAAKSQALEDDATMRAADLAEKRGPSSSPENPRKRPREDSDVEMVEDASRKEMTAACTPRRRIINLTSVLSLQEEINERGHETLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTRLSEELFYQILVYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLVGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYVSAESTLSGQQSEVPGSTANPWKWTVEHVIYKAFRSHLLPPKHFTEDGNILQLANLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_024982841.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mutL homolog 1 (MLH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982841.1

ORF Insert Sequence:

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLH1 ( XM_024982842.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838610.1 Bos taurus mutL homolog 1 (MLH1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa31338
Clone ID Related Accession (Same CDS sequence)	XM_024982839.1 , XM_024982840.1 , XM_024982841.1 , XM_024982842.1
Accession Version	XM_024982842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA mismatch repair protein Mlh1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838610.1
CDS	625..2178
Translation	MNGYISNANYSVKKCIFLLFINHRLVESASLRKAIETVYAAYLPKSTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEDSILERLQQHIESRLLGSNASRTYFTQTLLPGLPGPSGEAVKSTASVTSSSTAGSGDRVYAHQMVRTDCREQKLDAFLQPVSKALSSQPQAVVPEHRTDASSSGTRQQDEEMLELPAPAAVAAKSQALEDDATMRAADLAEKRGPSSSPENPRKRPREDSDVEMVEDASRKEMTAACTPRRRIINLTSVLSLQEEINERGHETLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTRLSEELFYQILVYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLVGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYVSAESTLSGQQSEVPGSTANPWKWTVEHVIYKAFRSHLLPPKHFTEDGNILQLANLPDLYKVFERC

Target ORF information:

RefSeq Version	XM_024982842.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mutL homolog 1 (MLH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982842.1

ORF Insert Sequence:

ATGAATGGCT ACATATCCAA TGCAAACTAT TCAGTGAAGA AGTGCATCTT CTTACTCTTC 
ATCAACCATC GTCTGGTAGA GTCAGCTTCC TTAAGAAAAG CCATAGAAAC GGTGTATGCA 
GCGTATTTGC CCAAAAGCAC ACACCCGTTC CTGTACCTCA GTCTAGAAAT AAGCCCCCAG 
AATGTGGATG TCAACGTGCA CCCCACGAAG CACGAGGTTC ACTTCCTGCA TGAGGACAGC 
ATCCTGGAAC GGCTGCAGCA GCACATCGAG AGCAGGCTCC TGGGCTCCAA CGCCTCCAGG 
ACCTACTTCA CCCAGACTTT ACTGCCAGGT CTTCCTGGCC CCTCTGGGGA AGCAGTTAAA 
TCCACAGCGA GTGTGACATC CTCATCCACT GCTGGAAGTG GTGACAGAGT GTATGCCCAC 
CAGATGGTCC GCACAGACTG CCGGGAACAG AAGCTCGATG CCTTCCTGCA GCCTGTGAGC 
AAGGCCCTGT CCAGTCAGCC CCAGGCTGTC GTCCCAGAGC ACAGGACAGA TGCTTCTAGC 
AGTGGGACCA GGCAGCAGGA CGAGGAGATG CTTGAACTCC CAGCTCCTGC TGCAGTGGCT 
GCGAAGAGTC AGGCCTTAGA GGACGATGCA ACAATGAGGG CTGCAGACCT GGCCGAGAAG 
AGAGGGCCCT CTTCCAGCCC CGAGAACCCC AGAAAGAGAC CTCGGGAAGA CTCTGATGTG 
GAAATGGTGG AGGATGCATC CCGAAAGGAG ATGACAGCCG CTTGTACCCC CCGGAGAAGG 
ATCATCAACC TTACCAGTGT TCTGAGTCTC CAGGAGGAGA TCAACGAGCG GGGCCATGAG 
ACTCTCCGGG AGATGCTGCA TAACCACTCC TTTGTGGGCT GCGTGAATCC TCAGTGGGCC 
TTGGCACAGC ATCAGACCAA GTTATACCTT CTCAACACCA CCAGACTTAG TGAAGAACTG 
TTCTACCAGA TTCTCGTTTA TGATTTTGCC AATTTTGGTG TTCTGAGGTT ATCGGAGCCA 
GCACCACTCT TTGACCTGGC CATGCTCGCC TTAGACAGTC CCGAGAGTGG CTGGACAGAG 
GAGGATGGTC CCAAGGAAGG ACTTGCTGAG TACATTGTTG AGTTTCTGAA AAAGAAGGCT 
GAGATGCTTG CAGACTACTT CTCTCTGGAG ATTGATGAGG AAGGGAACCT GGTTGGATTA 
CCCCTTCTGA TCGACAACTA TGTGCCCCCG CTGGAGGGTC TGCCTATCTT CATCCTCCGA 
CTGGCCACAG AGGTGAACTG GGATGAAGAA AAGGAATGTT TTGAAAGCCT CAGTAAAGAA 
TGTGCGATGT TTTACTCCAT CCGGAAGCAG TATGTGTCTG CAGAGTCAAC CCTTTCAGGC 
CAGCAGAGTG AAGTGCCTGG CTCCACTGCA AACCCCTGGA AGTGGACTGT GGAACACGTC 
ATTTATAAAG CCTTCCGCTC CCACCTGCTG CCTCCAAAAC ACTTCACAGA AGATGGGAAC 
ATCCTGCAGC TTGCTAACCT GCCTGATCTC TACAAAGTCT TCGAGAGGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Meiosis progression and donor age affect expression profile of DNA repair genes in bovine oocytes.
Zygote (Cambridge, England)23(1)11-8(2015 Feb)
Bilotto S,Boni R,Russo GL,Lioi MB

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MLH1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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