HERC4 cDNA ORF clone, Bos taurus(cattle)

The following HERC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa34230 XM_024986693.1
Latest version!
Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa34230 XM_010820591.3
Latest version!
Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52421 XM_010820592.2
Latest version!
Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa52422 XM_010820593.2
Latest version!
Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa88921 XM_015461000.1
Latest version!
Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBa40192 NM_001076894.3
Latest version!
Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa34229 XM_005226412.3
Latest version!
Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa34229 XM_005226412.2 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa34230 XM_010820591.2 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa40192 NM_001076894.2 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa52422 XM_010820593.1 Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa34230
    Clone ID Related Accession (Same CDS sequence) XM_010820591.3 , XM_024986693.1 , XM_010820591.2
    Accession Version XM_010820591.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCACTAAAT GACAAGGGCC AGGTGTATGC TTGGGGTCTC
    GATTCTGATG GACAACTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGAAAT
    ATTAAAAGTT TGTCGGATAT CCAGATAGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT ACTTGGAATC
    CCTTTCATGC AAATTGCGGC TGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATCTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCTGCACT AACCAAGGAA GGCGGAGTGT TTACTTTTGG AGCCGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTCAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGACAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTCACT GTGAAAGGAA ACTGGTTTCC TTATAATGGG
    CAGTGTCCAC CAGATTTTGA TTCTGAAGAA TATTTCTGCG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTATTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATATCCTG ATCCTTCGAA GCAGATCTGG ACTGTGAATG AAGCTTTAAT TCAAAAATGG
    CTCAGCTACC CCTCTGGAAG ATTTCCTGTA GAGATAGCCA ATGAAATTGA TGGAACATTT
    TCTTCATCTG GTTGTCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA
    ACGGGCACTA GATTTTCAGG GGTTGACATG AATGCTGCTA GACTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATAGCTCAG CAGGTGGCAG CTAGCTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGATTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG CGATTCCAAC AATTTCACCA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TGAACTTAGA AAAGGCACCG CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CGCTATTCCT CAAGATTGTA GAACTTTTTA AGGAGGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAGAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTA CATAGGGTAA ATGAGAAATC TGGACAGATT
    ATTCAATATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT ACGGAATGAT
    TATATCATCT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCGGTTAC AATCTGCACA TATCCATTTG TATTTGACGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAACCC TTGCCTAATT
    CTAGTGGTAC GTAGGGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAA
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGGGTAC GCAAAGAATT TTTCTTGCTT ATCATGAGGG AATTATTGGA TCCTAAATAT
    GGCATGTTTA GGTATTACGA AGATTCCAGA CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TATTCCACTT AATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TGCTAAAAAA GAAGCCATCC
    CTGGAAGATT TGAAAGAACT GGTCCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAC
    TATCCTGAAG ATGATGTTGA AGAGACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAACTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGAC AAGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCAG
    CTCTTTCAGC CTAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAATTGGAAA AGAATACAGA ATACAAAGGA GAATATTGGG CAGAACATCC TACAATAAAA
    ATGTTCTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TTTGTTATTT
    TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTAATACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ATACAGACAA AGAAACTCTA CGCTCTAAAC TGATTCAAGC TATTGATCAC
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818893.1
    CDS246..3209
    Translation

    Target ORF information:

    RefSeq Version XM_010820591.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820591.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCACTAAAT GACAAGGGCC AGGTGTATGC TTGGGGTCTC
    GATTCTGATG GACAACTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGAAAT
    ATTAAAAGTT TGTCGGATAT CCAGATAGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT ACTTGGAATC
    CCTTTCATGC AAATTGCGGC TGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATCTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCTGCACT AACCAAGGAA GGCGGAGTGT TTACTTTTGG AGCCGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTCAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGACAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTCACT GTGAAAGGAA ACTGGTTTCC TTATAATGGG
    CAGTGTCCAC CAGATTTTGA TTCTGAAGAA TATTTCTGCG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTATTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATATCCTG ATCCTTCGAA GCAGATCTGG ACTGTGAATG AAGCTTTAAT TCAAAAATGG
    CTCAGCTACC CCTCTGGAAG ATTTCCTGTA GAGATAGCCA ATGAAATTGA TGGAACATTT
    TCTTCATCTG GTTGTCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA
    ACGGGCACTA GATTTTCAGG GGTTGACATG AATGCTGCTA GACTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATAGCTCAG CAGGTGGCAG CTAGCTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGATTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG CGATTCCAAC AATTTCACCA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TGAACTTAGA AAAGGCACCG CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CGCTATTCCT CAAGATTGTA GAACTTTTTA AGGAGGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAGAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTA CATAGGGTAA ATGAGAAATC TGGACAGATT
    ATTCAATATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT ACGGAATGAT
    TATATCATCT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCGGTTAC AATCTGCACA TATCCATTTG TATTTGACGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAACCC TTGCCTAATT
    CTAGTGGTAC GTAGGGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAA
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGGGTAC GCAAAGAATT TTTCTTGCTT ATCATGAGGG AATTATTGGA TCCTAAATAT
    GGCATGTTTA GGTATTACGA AGATTCCAGA CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TATTCCACTT AATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TGCTAAAAAA GAAGCCATCC
    CTGGAAGATT TGAAAGAACT GGTCCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAC
    TATCCTGAAG ATGATGTTGA AGAGACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAACTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGAC AAGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCAG
    CTCTTTCAGC CTAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAATTGGAAA AGAATACAGA ATACAAAGGA GAATATTGGG CAGAACATCC TACAATAAAA
    ATGTTCTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TTTGTTATTT
    TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTAATACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ATACAGACAA AGAAACTCTA CGCTCTAAAC TGATTCAAGC TATTGATCAC
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34230
    Clone ID Related Accession (Same CDS sequence) XM_010820591.3 , XM_024986693.1 , XM_010820591.2
    Accession Version XM_024986693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCACTAAAT GACAAGGGCC AGGTGTATGC TTGGGGTCTC
    GATTCTGATG GACAACTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGAAAT
    ATTAAAAGTT TGTCGGATAT CCAGATAGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT ACTTGGAATC
    CCTTTCATGC AAATTGCGGC TGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATCTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCTGCACT AACCAAGGAA GGCGGAGTGT TTACTTTTGG AGCCGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTCAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGACAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTCACT GTGAAAGGAA ACTGGTTTCC TTATAATGGG
    CAGTGTCCAC CAGATTTTGA TTCTGAAGAA TATTTCTGCG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTATTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATATCCTG ATCCTTCGAA GCAGATCTGG ACTGTGAATG AAGCTTTAAT TCAAAAATGG
    CTCAGCTACC CCTCTGGAAG ATTTCCTGTA GAGATAGCCA ATGAAATTGA TGGAACATTT
    TCTTCATCTG GTTGTCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA
    ACGGGCACTA GATTTTCAGG GGTTGACATG AATGCTGCTA GACTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATAGCTCAG CAGGTGGCAG CTAGCTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGATTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG CGATTCCAAC AATTTCACCA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TGAACTTAGA AAAGGCACCG CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CGCTATTCCT CAAGATTGTA GAACTTTTTA AGGAGGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAGAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTA CATAGGGTAA ATGAGAAATC TGGACAGATT
    ATTCAATATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT ACGGAATGAT
    TATATCATCT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCGGTTAC AATCTGCACA TATCCATTTG TATTTGACGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAACCC TTGCCTAATT
    CTAGTGGTAC GTAGGGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAA
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGGGTAC GCAAAGAATT TTTCTTGCTT ATCATGAGGG AATTATTGGA TCCTAAATAT
    GGCATGTTTA GGTATTACGA AGATTCCAGA CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TATTCCACTT AATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TGCTAAAAAA GAAGCCATCC
    CTGGAAGATT TGAAAGAACT GGTCCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAC
    TATCCTGAAG ATGATGTTGA AGAGACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAACTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGAC AAGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCAG
    CTCTTTCAGC CTAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAATTGGAAA AGAATACAGA ATACAAAGGA GAATATTGGG CAGAACATCC TACAATAAAA
    ATGTTCTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TTTGTTATTT
    TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTAATACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ATACAGACAA AGAAACTCTA CGCTCTAAAC TGATTCAAGC TATTGATCAC
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842461.1
    CDS161..3124
    Translation

    Target ORF information:

    RefSeq Version XM_024986693.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCACTAAAT GACAAGGGCC AGGTGTATGC TTGGGGTCTC
    GATTCTGATG GACAACTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGAAAT
    ATTAAAAGTT TGTCGGATAT CCAGATAGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT ACTTGGAATC
    CCTTTCATGC AAATTGCGGC TGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATCTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCTGCACT AACCAAGGAA GGCGGAGTGT TTACTTTTGG AGCCGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTCAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGACAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTCACT GTGAAAGGAA ACTGGTTTCC TTATAATGGG
    CAGTGTCCAC CAGATTTTGA TTCTGAAGAA TATTTCTGCG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTATTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATATCCTG ATCCTTCGAA GCAGATCTGG ACTGTGAATG AAGCTTTAAT TCAAAAATGG
    CTCAGCTACC CCTCTGGAAG ATTTCCTGTA GAGATAGCCA ATGAAATTGA TGGAACATTT
    TCTTCATCTG GTTGTCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA
    ACGGGCACTA GATTTTCAGG GGTTGACATG AATGCTGCTA GACTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATAGCTCAG CAGGTGGCAG CTAGCTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGATTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG CGATTCCAAC AATTTCACCA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TGAACTTAGA AAAGGCACCG CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CGCTATTCCT CAAGATTGTA GAACTTTTTA AGGAGGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAGAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTA CATAGGGTAA ATGAGAAATC TGGACAGATT
    ATTCAATATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT ACGGAATGAT
    TATATCATCT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCGGTTAC AATCTGCACA TATCCATTTG TATTTGACGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAACCC TTGCCTAATT
    CTAGTGGTAC GTAGGGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAA
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGGGTAC GCAAAGAATT TTTCTTGCTT ATCATGAGGG AATTATTGGA TCCTAAATAT
    GGCATGTTTA GGTATTACGA AGATTCCAGA CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TATTCCACTT AATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TGCTAAAAAA GAAGCCATCC
    CTGGAAGATT TGAAAGAACT GGTCCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAC
    TATCCTGAAG ATGATGTTGA AGAGACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAACTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGAC AAGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCAG
    CTCTTTCAGC CTAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAATTGGAAA AGAATACAGA ATACAAAGGA GAATATTGGG CAGAACATCC TACAATAAAA
    ATGTTCTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TTTGTTATTT
    TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTAATACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ATACAGACAA AGAAACTCTA CGCTCTAAAC TGATTCAAGC TATTGATCAC
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34230
    Clone ID Related Accession (Same CDS sequence) XM_010820591.3 , XM_024986693.1 , XM_010820591.2
    Accession Version XM_010820591.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCACTAAAT GACAAGGGCC AGGTGTATGC TTGGGGTCTC
    GATTCTGATG GACAACTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGAAAT
    ATTAAAAGTT TGTCGGATAT CCAGATAGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT ACTTGGAATC
    CCTTTCATGC AAATTGCGGC TGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATCTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCTGCACT AACCAAGGAA GGCGGAGTGT TTACTTTTGG AGCCGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTCAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGACAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTCACT GTGAAAGGAA ACTGGTTTCC TTATAATGGG
    CAGTGTCCAC CAGATTTTGA TTCTGAAGAA TATTTCTGCG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTATTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATATCCTG ATCCTTCGAA GCAGATCTGG ACTGTGAATG AAGCTTTAAT TCAAAAATGG
    CTCAGCTACC CCTCTGGAAG ATTTCCTGTA GAGATAGCCA ATGAAATTGA TGGAACATTT
    TCTTCATCTG GTTGTCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA
    ACGGGCACTA GATTTTCAGG GGTTGACATG AATGCTGCTA GACTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATAGCTCAG CAGGTGGCAG CTAGCTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGATTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG CGATTCCAAC AATTTCACCA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TGAACTTAGA AAAGGCACCG CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CGCTATTCCT CAAGATTGTA GAACTTTTTA AGGAGGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAGAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTA CATAGGGTAA ATGAGAAATC TGGACAGATT
    ATTCAATATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT ACGGAATGAT
    TATATCATCT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCGGTTAC AATCTGCACA TATCCATTTG TATTTGACGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAACCC TTGCCTAATT
    CTAGTGGTAC GTAGGGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAA
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGGGTAC GCAAAGAATT TTTCTTGCTT ATCATGAGGG AATTATTGGA TCCTAAATAT
    GGCATGTTTA GGTATTACGA AGATTCCAGA CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TATTCCACTT AATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TGCTAAAAAA GAAGCCATCC
    CTGGAAGATT TGAAAGAACT GGTCCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAC
    TATCCTGAAG ATGATGTTGA AGAGACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAACTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGAC AAGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCAG
    CTCTTTCAGC CTAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAATTGGAAA AGAATACAGA ATACAAAGGA GAATATTGGG CAGAACATCC TACAATAAAA
    ATGTTCTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TTTGTTATTT
    TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTAATACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ATACAGACAA AGAAACTCTA CGCTCTAAAC TGATTCAAGC TATTGATCAC
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818893.1
    CDS118..3081
    Translation

    Target ORF information:

    RefSeq Version XM_010820591.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820591.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATTGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCACTAAAT GACAAGGGCC AGGTGTATGC TTGGGGTCTC
    GATTCTGATG GACAACTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGAAAT
    ATTAAAAGTT TGTCGGATAT CCAGATAGTA CAGGTTGCTT GTGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCAAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAATTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT ACTTGGAATC
    CCTTTCATGC AAATTGCGGC TGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATCTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCTGCACT AACCAAGGAA GGCGGAGTGT TTACTTTTGG AGCCGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCAAGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTCAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGACAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTCACT GTGAAAGGAA ACTGGTTTCC TTATAATGGG
    CAGTGTCCAC CAGATTTTGA TTCTGAAGAA TATTTCTGCG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTATTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATATCCTG ATCCTTCGAA GCAGATCTGG ACTGTGAATG AAGCTTTAAT TCAAAAATGG
    CTCAGCTACC CCTCTGGAAG ATTTCCTGTA GAGATAGCCA ATGAAATTGA TGGAACATTT
    TCTTCATCTG GTTGTCTAAA TGGAAGTTTT TTAGCTGTTA GCAATGATGA TCACTATAGA
    ACGGGCACTA GATTTTCAGG GGTTGACATG AATGCTGCTA GACTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATAGCTCAG CAGGTGGCAG CTAGCTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGCTC CTTACCTGAT GTTGAAGCAT TGAGATTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG CGATTCCAAC AATTTCACCA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TGAACTTAGA AAAGGCACCG CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CGCTATTCCT CAAGATTGTA GAACTTTTTA AGGAGGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAGAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTA CATAGGGTAA ATGAGAAATC TGGACAGATT
    ATTCAATATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT ACGGAATGAT
    TATATCATCT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCGGTTAC AATCTGCACA TATCCATTTG TATTTGACGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTTCTCCCA GTGATTGAAT CTGTGAACCC TTGCCTAATT
    CTAGTGGTAC GTAGGGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAA
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGGGTAC GCAAAGAATT TTTCTTGCTT ATCATGAGGG AATTATTGGA TCCTAAATAT
    GGCATGTTTA GGTATTACGA AGATTCCAGA CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TATTCCACTT AATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCATTTTCC TTTGGCTTTA TATAAGAAAC TGCTAAAAAA GAAGCCATCC
    CTGGAAGATT TGAAAGAACT GGTCCCTGAT GTTGGGAGAA GCATGCAACA GTTACTGGAC
    TATCCTGAAG ATGATGTTGA AGAGACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAACTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGAC AAGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTATTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA AGTCCTTCAG
    CTCTTTCAGC CTAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAATTGGAAA AGAATACAGA ATACAAAGGA GAATATTGGG CAGAACATCC TACAATAAAA
    ATGTTCTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TTTGTTATTT
    TTGACAGGTA GTGATCGCAT TCCTATTCTT GGTATGAAGA GTCTAATACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA GTATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ATACAGACAA AGAAACTCTA CGCTCTAAAC TGATTCAAGC TATTGATCAC
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52421
    Clone ID Related Accession (Same CDS sequence) XM_010820592.2
    Accession Version XM_010820592.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA CATTTGAAGA CAGTGATTTA TTCCACTTAA TTGGTGTTAT CTGTGGATTA
    GCAATTTATA ATTTTACTAT TGTGGACCTC CATTTTCCTT TGGCTTTATA TAAGAAACTG
    CTAAAAAAGA AGCCATCCCT GGAAGATTTG AAAGAACTGG TCCCTGATGT TGGGAGAAGC
    ATGCAACAGT TACTGGACTA TCCTGAAGAT GATGTTGAAG AGACATTTTG TCTTAATTTT
    ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AACTGGTTCT AAATGGTGCA
    GACACAGCTG TTAACAAACA AAATCGACAA GAGTTTGTTG ATGCTTATGT GGATTACATA
    TTCAATAAAT CAGTGGCTTC CTTATTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT
    GGAGGAAAAG TCCTTCAGCT CTTTCAGCCT AATGAACTAC AAGCAATGGT TATTGGAAAC
    ACAAATTATG ATTGGAAGGA ATTGGAAAAG AATACAGAAT ACAAAGGAGA ATATTGGGCA
    GAACATCCTA CAATAAAAAT GTTCTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA
    AAACAATTTT TGTTATTTTT GACAGGTAGT GATCGCATTC CTATTCTTGG TATGAAGAGT
    CTAATACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAGT ATCTCCCAGT TTCCCATACC
    TGTTTTAATC TTCTGGATCT TCCAAAATAT ACAGACAAAG AAACTCTACG CTCTAAACTG
    ATTCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818894.1
    CDS408..3269
    Translation

    Target ORF information:

    RefSeq Version XM_010820592.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820592.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA CATTTGAAGA CAGTGATTTA TTCCACTTAA TTGGTGTTAT CTGTGGATTA
    GCAATTTATA ATTTTACTAT TGTGGACCTC CATTTTCCTT TGGCTTTATA TAAGAAACTG
    CTAAAAAAGA AGCCATCCCT GGAAGATTTG AAAGAACTGG TCCCTGATGT TGGGAGAAGC
    ATGCAACAGT TACTGGACTA TCCTGAAGAT GATGTTGAAG AGACATTTTG TCTTAATTTT
    ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AACTGGTTCT AAATGGTGCA
    GACACAGCTG TTAACAAACA AAATCGACAA GAGTTTGTTG ATGCTTATGT GGATTACATA
    TTCAATAAAT CAGTGGCTTC CTTATTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT
    GGAGGAAAAG TCCTTCAGCT CTTTCAGCCT AATGAACTAC AAGCAATGGT TATTGGAAAC
    ACAAATTATG ATTGGAAGGA ATTGGAAAAG AATACAGAAT ACAAAGGAGA ATATTGGGCA
    GAACATCCTA CAATAAAAAT GTTCTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA
    AAACAATTTT TGTTATTTTT GACAGGTAGT GATCGCATTC CTATTCTTGG TATGAAGAGT
    CTAATACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAGT ATCTCCCAGT TTCCCATACC
    TGTTTTAATC TTCTGGATCT TCCAAAATAT ACAGACAAAG AAACTCTACG CTCTAAACTG
    ATTCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52422
    Clone ID Related Accession (Same CDS sequence) XM_010820593.1 , XM_010820593.2
    Accession Version XM_010820593.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGACAAG AGTTTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818895.1
    CDS408..3017
    Translation

    Target ORF information:

    RefSeq Version XM_010820593.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820593.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGACAAG AGTTTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52422
    Clone ID Related Accession (Same CDS sequence) XM_010820593.1 , XM_010820593.2
    Accession Version XM_010820593.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGACAAG AGTTTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818895.1
    CDS432..3041
    Translation

    Target ORF information:

    RefSeq Version XM_010820593.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820593.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGACAAG AGTTTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88921
    Clone ID Related Accession (Same CDS sequence) XM_015461000.1
    Accession Version XM_015461000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGGTAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316486.1
    CDS408..2048
    Translation

    Target ORF information:

    RefSeq Version XM_015461000.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGGTAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40192
    Clone ID Related Accession (Same CDS sequence) NM_001076894.2 , NM_001076894.3
    Accession Version NM_001076894.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC123842.1, EE248061.1 and CR850193.2. On Jan 4, 2008 this sequence version replaced NM_001076894.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAACTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGAC AAGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA AGTCCTTCAG CTCTTTCAGC CTAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAATTGGAAA AGAATACAGA ATACAAAGGA
    GAATATTGGG CAGAACATCC TACAATAAAA ATGTTCTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TTTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT
    GGTATGAAGA GTCTAATACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA GTATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGACAA AGAAACTCTA
    CGCTCTAAAC TGATTCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070362.2
    CDS358..3531
    Translation

    Target ORF information:

    RefSeq Version NM_001076894.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076894.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAACTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGAC AAGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA AGTCCTTCAG CTCTTTCAGC CTAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAATTGGAAA AGAATACAGA ATACAAAGGA
    GAATATTGGG CAGAACATCC TACAATAAAA ATGTTCTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TTTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT
    GGTATGAAGA GTCTAATACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA GTATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGACAA AGAAACTCTA
    CGCTCTAAAC TGATTCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40192
    Clone ID Related Accession (Same CDS sequence) NM_001076894.2 , NM_001076894.3
    Accession Version NM_001076894.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-01
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000028.1. On Jan 18, 2018 this sequence version replaced NM_001076894.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571265.32745.1, SRR5571257.49826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAACTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGAC AAGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA AGTCCTTCAG CTCTTTCAGC CTAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAATTGGAAA AGAATACAGA ATACAAAGGA
    GAATATTGGG CAGAACATCC TACAATAAAA ATGTTCTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TTTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT
    GGTATGAAGA GTCTAATACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA GTATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGACAA AGAAACTCTA
    CGCTCTAAAC TGATTCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070362.2
    CDS358..3531
    Translation

    Target ORF information:

    RefSeq Version NM_001076894.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076894.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCGGTTAC AATCTGCACA
    TATCCATTTG TATTTGACGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTTCTCCCA
    GTGATTGAAT CTGTGAACCC TTGCCTAATT CTAGTGGTAC GTAGGGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAA AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGGGTAC GCAAAGAATT TTTCTTGCTT
    ATCATGAGGG AATTATTGGA TCCTAAATAT GGCATGTTTA GGTATTACGA AGATTCCAGA
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TATTCCACTT AATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCATTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTAAAAAA GAAGCCATCC CTGGAAGATT TGAAAGAACT GGTCCCTGAT
    GTTGGGAGAA GCATGCAACA GTTACTGGAC TATCCTGAAG ATGATGTTGA AGAGACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAACTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGAC AAGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTATTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA AGTCCTTCAG CTCTTTCAGC CTAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAATTGGAAA AGAATACAGA ATACAAAGGA
    GAATATTGGG CAGAACATCC TACAATAAAA ATGTTCTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TTTGTTATTT TTGACAGGTA GTGATCGCAT TCCTATTCTT
    GGTATGAAGA GTCTAATACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA GTATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ATACAGACAA AGAAACTCTA
    CGCTCTAAAC TGATTCAAGC TATTGATCAC AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34229
    Clone ID Related Accession (Same CDS sequence) XM_005226412.3 , XM_005226412.2
    Accession Version XM_005226412.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC GGTTACAATC TGCACATATC CATTTGTATT TGACGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAACCCTTGC
    CTAATTCTAG TGGTACGTAG GGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAAAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GGGTACGCAA AGAATTTTTC TTGCTTATCA TGAGGGAATT ATTGGATCCT
    AAATATGGCA TGTTTAGGTA TTACGAAGAT TCCAGACTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTATT CCACTTAATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTGCT AAAAAAGAAG
    CCATCCCTGG AAGATTTGAA AGAACTGGTC CCTGATGTTG GGAGAAGCAT GCAACAGTTA
    CTGGACTATC CTGAAGATGA TGTTGAAGAG ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAA CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGACAAGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC
    CTTCAGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAT TGGAAAAGAA TACAGAATAC AAAGGAGAAT ATTGGGCAGA ACATCCTACA
    ATAAAAATGT TCTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTTTG
    TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT AATACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATATAC AGACAAAGAA ACTCTACGCT CTAAACTGAT TCAAGCTATT
    GATCACAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226469.1
    CDS432..3581
    Translation

    Target ORF information:

    RefSeq Version XM_005226412.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226412.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC GGTTACAATC TGCACATATC CATTTGTATT TGACGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAACCCTTGC
    CTAATTCTAG TGGTACGTAG GGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAAAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GGGTACGCAA AGAATTTTTC TTGCTTATCA TGAGGGAATT ATTGGATCCT
    AAATATGGCA TGTTTAGGTA TTACGAAGAT TCCAGACTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTATT CCACTTAATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTGCT AAAAAAGAAG
    CCATCCCTGG AAGATTTGAA AGAACTGGTC CCTGATGTTG GGAGAAGCAT GCAACAGTTA
    CTGGACTATC CTGAAGATGA TGTTGAAGAG ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAA CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGACAAGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC
    CTTCAGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAT TGGAAAAGAA TACAGAATAC AAAGGAGAAT ATTGGGCAGA ACATCCTACA
    ATAAAAATGT TCTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTTTG
    TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT AATACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATATAC AGACAAAGAA ACTCTACGCT CTAAACTGAT TCAAGCTATT
    GATCACAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa34229
    Clone ID Related Accession (Same CDS sequence) XM_005226412.3 , XM_005226412.2
    Accession Version XM_005226412.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC GGTTACAATC TGCACATATC CATTTGTATT TGACGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAACCCTTGC
    CTAATTCTAG TGGTACGTAG GGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAAAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GGGTACGCAA AGAATTTTTC TTGCTTATCA TGAGGGAATT ATTGGATCCT
    AAATATGGCA TGTTTAGGTA TTACGAAGAT TCCAGACTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTATT CCACTTAATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTGCT AAAAAAGAAG
    CCATCCCTGG AAGATTTGAA AGAACTGGTC CCTGATGTTG GGAGAAGCAT GCAACAGTTA
    CTGGACTATC CTGAAGATGA TGTTGAAGAG ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAA CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGACAAGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC
    CTTCAGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAT TGGAAAAGAA TACAGAATAC AAAGGAGAAT ATTGGGCAGA ACATCCTACA
    ATAAAAATGT TCTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTTTG
    TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT AATACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATATAC AGACAAAGAA ACTCTACGCT CTAAACTGAT TCAAGCTATT
    GATCACAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226469.1
    CDS408..3557
    Translation

    Target ORF information:

    RefSeq Version XM_005226412.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226412.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC TTCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTTCG AGATGTAGGA
    TGTGGACTCA GACATACTGT ATTTGTTCTG GATGATGGAA CAGTGTACAC GTGTGGATGT
    AATGATCTAG GGCAGCTAGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATTGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCACTAAAT
    GACAAGGGCC AGGTGTATGC TTGGGGTCTC GATTCTGATG GACAACTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCGGATAT CCAGATAGTA
    CAGGTTGCTT GTGGTTACTA TCATTCACTT GCACTTTCTA AAGCAAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAATTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT ACTTGGAATC CCTTTCATGC AAATTGCGGC TGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATCTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCTGCACT AACCAAGGAA
    GGCGGAGTGT TTACTTTTGG AGCCGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCAAGGAA AGTTTTTGAA CTTATGGGAA GCATTGTCAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGACAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTCACT
    GTGAAAGGAA ACTGGTTTCC TTATAATGGG CAGTGTCCAC CAGATTTTGA TTCTGAAGAA
    TATTTCTGCG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTATTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATATCCTG ATCCTTCGAA GCAGATCTGG
    ACTGTGAATG AAGCTTTAAT TCAAAAATGG CTCAGCTACC CCTCTGGAAG ATTTCCTGTA
    GAGATAGCCA ATGAAATTGA TGGAACATTT TCTTCATCTG GTTGTCTAAA TGGAAGTTTT
    TTAGCTGTTA GCAATGATGA TCACTATAGA ACGGGCACTA GATTTTCAGG GGTTGACATG
    AATGCTGCTA GACTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATAGCTCAG
    CAGGTGGCAG CTAGCTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGCTC CTTACCTGAT
    GTTGAAGCAT TGAGATTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG CGATTCCAAC
    AATTTCACCA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TGAACTTAGA AAAGGCACCG
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CGCTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAGGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAGAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTA
    CATAGGGTAA ATGAGAAATC TGGACAGATT ATTCAATATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT ACGGAATGAT TATATCATCT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC GGTTACAATC TGCACATATC CATTTGTATT TGACGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTT CTCCCAGTGA TTGAATCTGT GAACCCTTGC
    CTAATTCTAG TGGTACGTAG GGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAAAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GGGTACGCAA AGAATTTTTC TTGCTTATCA TGAGGGAATT ATTGGATCCT
    AAATATGGCA TGTTTAGGTA TTACGAAGAT TCCAGACTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTATT CCACTTAATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA TTTTCCTTTG GCTTTATATA AGAAACTGCT AAAAAAGAAG
    CCATCCCTGG AAGATTTGAA AGAACTGGTC CCTGATGTTG GGAGAAGCAT GCAACAGTTA
    CTGGACTATC CTGAAGATGA TGTTGAAGAG ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAA CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGACAAGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TATTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAAGTC
    CTTCAGCTCT TTCAGCCTAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAT TGGAAAAGAA TACAGAATAC AAAGGAGAAT ATTGGGCAGA ACATCCTACA
    ATAAAAATGT TCTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTTTG
    TTATTTTTGA CAGGTAGTGA TCGCATTCCT ATTCTTGGTA TGAAGAGTCT AATACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAGTAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATATAC AGACAAAGAA ACTCTACGCT CTAAACTGAT TCAAGCTATT
    GATCACAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.