APC cDNA ORF clone, Bos taurus(cattle)

The following APC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219624 XM_024997644.1
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa219625 XM_024997645.1
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa39178 NM_001075986.2
Latest version!
Bos taurus adenomatous polyposis coli (APC), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219610 XM_015472979.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219611 XM_015472982.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219611 XM_015472980.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219611 XM_015472981.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219612 XM_015472983.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219613 XM_010808816.3
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219614 XM_024997640.1
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219615 XM_005211196.4
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219616 XM_024997641.1
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219616 XM_005211197.4
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219617 XM_015472986.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219618 XM_015472987.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219619 XM_024997642.1
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219620 XM_005211202.4
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219621 XM_005211203.4
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219622 XM_015472988.2
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa219623 XM_024997643.1
Latest version!
Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22863 XM_005211196.3
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22864 XM_015472985.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22864 XM_005211197.3
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22868 XM_005211202.3
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22869 XM_005211203.3
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52405 XM_010808816.2
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91575 XM_015472979.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91576 XM_015472981.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91576 XM_015472982.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91576 XM_015472980.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91577 XM_015472983.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91578 XM_015472984.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91579 XM_015472986.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91580 XM_015472987.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91581 XM_015472988.1
Latest version!
Bos taurus adenomatous polyposis coli (APC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219624
    Clone ID Related Accession (Same CDS sequence) XM_024997644.1
    Accession Version XM_024997644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAAGCTTT CGTTTGATGA AGAGCATAGA CATGCCATGA ATGAGCTTGG TAGGAAGGCT 
    ACCCGGGGCA TTTCATCCCA GGAGCTAGGG CAGGGGCTTT CAGGGGGACT ACAGGCCATT
    GCAGAATTAT TGCAAGTGGA CTGTGAAATG TATGGGCTTA CTAATGACCA CTACAGTATT
    ACTTTAAGAC GATATGCAGG AATGGCTTTG ACAAACTTGA CTTTCGGAGA TGTAGCCAAC
    AAGGCTACTC TCTGCTCTAT GAAAGGCTGC ATGAGAGCGC TCGTGGCTCA GCTCCAGTCT
    GAAAGCGAGG ACTTGCAGCA GGTTATTGCG AGTGTTTTGA GGAATCTGTC TTGGAGAGCA
    GATGTAAATA GTAAAAAGAC TTTGCGTGAA GTTGGAAGTG TGAAAGCATT GATGGAATGT
    GCTTTGGAAG TGAAAAAGGA ATCAACCCTC AAAAGTGTAT TGAGTGCCTT ATGGAACTTG
    TCAGCACACT GCACTGAGAA TAAAGCTGAC ATATGTGCTG TGGATGGTGC GCTTGCATTT
    TTGGTTGGCA CTCTCACTTA TCGCAGCCAG ACCAATACTT TAGCCATTAT TGAAAGTGGA
    GGTGGTATAT TACGGAATGT ATCCAGCTTG ATAGCTACAA ATGAGGACCA CAGGCAAATC
    CTAAGAGAGA ATAATTGCTT ACAGACCTTA TTACAACACT TGAAATCTCA CAGTTTGACA
    ATAGTCAGCA ATGCATGCGG AACCTTGTGG AATCTCTCAG CAAGAAACCC TAAAGACCAG
    GAAGCATTGT GGGACATGGG AGCAGTCAGC ATGCTCAAGA ACCTCATTCA TTCAAAGCAC
    AAGATGATTG CTATGGGAAG TGCTGCAGCT TTAAGGAATC TCATGGCAAA TAGACCTGCA
    AAGTATAAAG ATGCCAATAT CATGTCTCCT GGTTCAAGTT TGCCTTCTCT ACATGTCAGG
    AAACAAAAGG CCCTGGAAGC AGAATTAGAT GCTCAGCATT TATCAGAAAC TTTTGACAAT
    ATTGACAATT TAAGTCCCAA GGCATCTCAT CGTAGCAAGC AGAGACACAA GCAAAATCTC
    TATGGTGACT ATGTTTTTGA CACCAATCGA CATGATGATA ACAGGTCAGA CAATTTTAAT
    ACTGGAAACA TGACTGTCCT ATCACCGTAC TTAAACACTA CAGTATTGCC CAGCTCTTCT
    TCATCAAGGG GAAGTTTAGA TAGCTCTCGT TCTGAGAAAG ATAGAAGTCT GGAGAGAGAA
    CGAGGTATTA GCCTAGGCAA CTATCACCCA GCAACAGAAA ACCCAGGAAC CTCTTCAAAG
    CGAGGTTTGC AGATTTCTAC CACTGCAGCC CAGATTGCCA AAGTCATGGA AGAAGTATCA
    GCTATTCATA CCTCTCAGGA AGACAGAAGT TCTGGGTCTA CCACAGAACT ACACTGTGGG
    ACAGATGAGA GGAATGCACT AAGAAGAAGC TCTACCACCC ACACACATGC AAACACGTAC
    AACTTTACCA AGTCAGAAAA CTCAAACAGA ACATGTCCAA TACCATATGC CAAAGTAGAA
    TATAAGAGAT CTTCAAATGA TAGTTTAAAT AGTGTCAGCA GTAGTGATGG TTATGGTAAA
    AGAGGTCAGA TGAAACCTTC AATTGAATCC TATTCTGAAG ATGATGAAAG TAAATTTTGC
    AGCTATGGTC AGTATCCAGC TGACCTAGCC CATAAAATAC ATAGTGCAAA TCATATGGAT
    GATAATGATG GAGAACTAGA TACACCAATA AATTACAGTC TCAAATATTC CGATGAACAG
    TTGAACTCTG GGAGGCAGAG CCCTTCACAG AATGAAAGAT GGGCAAGACC CAAACATATA
    CTAGAAGATG AAATAAAACC GAATGAGCAG AGACAATCAA GGAGTCAAAG CACAGCTTAT
    CCCGTGTATC CTGAGAGCAC TGATGATAAA CACCTCAAGT TCCAACCACA CTTTGGGCAG
    CAAGAATGTG TTTCCCCATA CAGGTCAAGA GCAGCCAATG GATCTGAAAC AAATCGAGTA
    GGCTCTAATC ATGGAATTAG TCAAAATGTG AACCAGTCTT TGTGTCAAGA AGATGACTAT
    GAAGACGATA AACCAACCAA CTATAGCGAA CGTTACTCTG AGGAAGGGCA GCATGAGGAA
    GAAGAGAGGC CAACCAATTA TAGCATAAAA TACAGTGAAG AAAAACATCA CGTGGATCAG
    CCTATTGATT ATAGTTTAAA ATACACCACA GACATTCCTT CTTCACAGAA ACCAGCATTT
    TCATTCTCAA AGAATTCATC TGGACAGAGC ACAAAAACTG AACACATCTC TTCAAGCAGC
    GAGAATACAT CCACAACTTC ATCTAATGCC AAGAGGCAGA ATCAGCTGCA TCCAAGCTCA
    GCACAGAGCA GAAGTGGTCA GACCCCAAAA GCCACCTCTT CCTCTTGCAA AGTCCCCTCT
    ATCAACCAAG AAACAATACA GACTTACTGT GTAGAAGATA CCCCAATATG TTTTTCAAGA
    TGCAGTTCAT TATCATCTCT GTCATCTGCT GAAGATGAAG TAGGGTGTGA TCAGACAACA
    CAAGAAGCAG AGTCTGCTAA CACTCTGCAA ATAGCTGAAA TCAAGGACAA CAGCGGACCT
    AGGTCAAATG AAGATTCTGT GAGTAAAGTT CCAGCAGGGT CACAGCACAT TAGAACCAAA
    TCCAGCAGAC TCCAGGCTTC TGGTCTGTCT TCAGAGTCAG CCAGGCACAA AGCTGTTGAA
    TTTTCTTCAG GGGCCAAATC TCCTTCCAAG AGCGGTGCTC AGACACCCAA AAGTCCACCA
    GAGCACTACG TTCAGGAGAC CCCACTCATG TTTAGCAGAT GTACTTCAGT CAGTTCACTC
    GACAGTTTTG AGAGTCGCTC GATTGCCAGC TCTGTTCAGA GTGAACCCTG CAGTGGAATG
    GTAAGTGGCA TCATAAGCCC CAGTGACCTC CCAGATAGCC CTGGACAAAC CATGCCACCA
    AGCAGAAGCA AAACCCCTCC GCCACCGCCT CCGCCTCCTC CTCAGACAGT TCAAACGAAG
    CAGGAAGTAC CTAAAAATAA AGCACCTAGT GCTGAAAAGA GAGAAAGTGG ACCCAAGCAA
    GCTGCTGTAA ATGCTGCAGT ACAAAGGGTC CAGGTTCTTC CAGAGGCTGA TACTCTGTTA
    CATTTTGCCA CAGAGAGTAC TCCTGATGGA TTTTCTTGTT CATCTAGCCT GAGCGCTCTG
    AGCCTTGATG AGCCATTTAT TCAGAAAGAT GTGGAACTAA GAATAATGCC TCCAGTTCAG
    GAAAATGACA ACGGGAATGA AACAGAAAGT GAGCAGCCTG AAGAATCAAA TGAAAACCAG
    GAAAAAGAGG CAGAAAAACC CACTGATTCT GAAAAAGATC TTTTAGATGA GTCAGATGAT
    GATGATATTG AAATACTAGA AGAGTGTATA ATTTCTGCCA TGCCAACAAA ATCTTCACGC
    AAAGCCAAAA AACCAGCCCA GACTACTTCA AAATTACCTC CACCTGTGGC AAGGAAACCA
    AGTCAGCTCC CTGTATACAA ACTTCTGCCA TCGCAAAACA GGTTACAGGC ACAAAAGCAT
    GTTAGTTTTA CACCAGGAGA TGATATGCCA CGGGTGTATT GTGTAGAAGG GACACCTATA
    AACTTTTCCA CAGCTACATC TCTGAGTGAT CTAACGATAG AATCCCCTCC AAATGAGTTA
    GCTGCTGGAG AAGGGGTTAG AGCAGGGGCA CAGTCAAGTG AGTTTGAAAA ACGAGATACC
    ATTCCTACTG AAGGCAGAAG TACAGATGAG GCTCAACGAG GGAAAGCCTC ATCTGTCACT
    GTGCCTGAAC TGGACGACAG CAAAACAGAA GAAGGTGATA TTCTTGCAGA ATGCATTAAT
    TCTGCCATGC CCAAAGGAAA AAGTCACAAG CCTTTCCGTG TGAAAAAGAT CATGGACCAG
    GTCCAGCAAG CATCTATGTC TTCATCTGGA ACTAACAAAA ATCAATTAGA TGGTAAGACG
    AAGAAACCTA CTTCACCAGT AAAACCTATA CCACAAAATA CTGAATACAG GACACGTGTA
    AGAAAAAATA CAGACTCAAA AAATAATCTA AATGCAGAAA GAAATTTCTC GGAAAACAAA
    GACTCAAAGA AACAGCACTT GAAAAATAAT TCCAAGGACT TCAATGATAA ACTGCCAAAT
    AATGAAGACA GAGTCAGAGG AAGTTTTACT TTTGATTCAC CCCATCATTA CACGCCCATT
    GAAGGCACTC CGTACTGTTT TTCACGAAAT GACTCTCTGA GTTCTCTAGA TTTTGATGAT
    GATGATGTTG ACCTTTCCAG GGAAAAGGCT GAATTAAGAA AGGGGAAGGA AAATAAAGAA
    TCAGAAGCTA AAGTGACCAA CCACACAGAA CTAACCTCAA ACCAACAATC AGCTAGTAAG
    ACACCAGCTG TTACAAAGCA GCCAATAAAT AGAGGTCAGT CTAAACCCGT GCTGCAGAAG
    CAGTCCACTT TTCCCCAGTC TTCCAAAGAT ATACCAGACA GAGGGGCAGC AACAGATGAG
    AAATTACAGA ATTTCGCTAT TGAAAATACT CCAGTTTGCT TTTCTCGAAA TTCCTCTCTA
    AGCTCTCTTA GTGACATTGA TCAAGAAAAC AACAACAACA AGGAAAATGA ACCTGTCAAA
    GAGACAGAGC CCCCTGCCTC ACAGGGAGAA CCAGGTAAAC CCCAGGCCTC AGGTTATGCT
    CCTAAATCGT TTCACGTGGA AGACACCCCT GTTTGTTTCT CAAGAAACAG TTCTCTCAGT
    TCTCTTAGTA TTGATTCTGA AGATGACCTG CTGCAGGAAT GTATAAGTTC TGCAATGCCA
    AAAAAGAAAA AGCCTTCAAG GCTCAAGCCC GATAATGAAA AGCATAGTCC CAGAAATATG
    GGTGGCATAT TAGCAGAAGA TTTGACACTC GATTTGAAAG ATATACAGAG ACCAGATTCA
    GAACATGGTT TATCCCCAGA TTCAGAAAAT TTTGATTGGA AAGCTATTCA GGAAGGTGCA
    AATTCCATAG TAAGTAGTTT ACATCAAGCT GCTGCCGCTG CATGTTTATC TCGACAAGCT
    TCATCTGATT CAGATTCTAT CCTTTCATTG AAATCGGGTA TCTCTCTGGG ATCACCATTT
    CACCTTACAC CTGATCAAGA AGAAAAACCC TTTACGAGTA ATAAAGGTCC ACGAATTCTA
    AAACCTGGGG AGAAAAGTAC ATTGGAAACT AAAAAAATAG AATCTGAAAA TAAAGGAATA
    AAAGGAGGCA AAAAGGTTTA TAAAAGTTTG ATTACTGGAA AAGTTCGATC TAATTCGGAA
    ATTTCGAGCC AAATGAAACA ACCCCTTCAA ACAAACATGC CTTCAATCTC TCGAGGTAGG
    ACAATGATTC ATATTCCAGG AGTTCGGAAT AGCTCTTCAA GTACAAGCCC AGTTTCTAAA
    AAAGGCCCGC CCCTCAAGAC TCCAGCCTCC AAAAGCCCTA GTGAAGGTCA GCCGGCTACC
    ACCTCTCCCA GAGGAACCAA GCCATCAGTG AAGTCAGAAT TAAGCCCTGT TACAAGGCAG
    GCATCCCAGA CAGCAGGATC AAACAAAGGA CCTTCTAGAT CAGGATCTAG AGATTCCACT
    CCTTCAAGAC CTGCCCAGCA ACCATTAAGT AGACCAATGC AGTCTCCAGG GCGAAACTCA
    ATCTCTCCTG GTAGAAATGG AATAAGTCCC CCTAACAAAT TATCTCAACT ACCAAGGACG
    TCATCCCCTA GTACTGCTTC AACTAAGTCC TCAGGTTCTG GGAAAATGTC TTACACATCT
    CCTGGCAGAC AGATGAGCCA GCAGAACCTC ACCAAACAAA CAGGCTTATC CAAGAATGGC
    AGTGGTATCC CAAGAAGTGA ATCTGCCTCC AAAGGGCTAA ATCAAATGAG TAATAGTAAT
    GGATCCAATA AAAAAGTAGA ACTTTCTAGA ATGTCTTCAA CAAAGTCAAG TGGAAGTGAA
    TCCGATAGGT CAGAGAGACC TGTATTAGTA CGCCAATCAA CTTTCATCAA AGAAGCTCCA
    AGCCCAACCC TAAGGAGAAA ACTGGAGGAA TCTGCTTCAT TTGAATCTCT TTCTCCATCT
    TCCAGACCCG ATTCTCCCAC ACGGTCCCAG GCTCATACTC CAGTTTTAAG TCCTTCCCTT
    CCTGATATGT CTCTATCTAC ACATTCGTCT CTTCAGTCTG GTGGATGGCG AAAACTCCCA
    CCTAACCTCA GTCCCACCAT AGAGTATAAT GATGGAAGAC CAGTAAAGCG CCATGATATA
    GCACGCTCTC ATTCCGAAAG TCCTTCCAGA CTTCCCATCA ATAGGTCAGG GACCTGGAAA
    CGTGAGCACA GCAAACACTC ATCATCACTT CCTCGAGTAA GCACTTGGAG AAGAACTGGA
    AGTTCATCCT CAATTCTTTC TGCTTCATCA GAATCTAGTG AAAAGGCAAA AAGTGAGGAC
    GAAAAACAAG TGAACTCTAT TTCAGGAAGC AAACAAACTA AAGAAAACCA GGTATCCACA
    AAAGGAACAT GGAGAAAAAT AAAAGAAAGT GAAATTTCTC CCACAAATAG TACTTCTCAG
    ACCACTTCTT CAGGTGCTGC AAATGGTGCT GAATCAAAGA CTCTGATTTA TCAAATGGCA
    CCTGCTGTTT CTAAAACAGA GGATGTTTGG GTGAGAATTG AGGATTGCCC CATTAACAAC
    CCTAGATCTG GAAGATCTCC AACAGGAAAT ACTCCCCCCG TGATTGACAC TGTTTCAGAA
    AAGGGAAACC CGAACCCTAA AGATTCAAAA GATAATCAGG GAAAACAAAA TGTGAGCAAT
    GGTAGTGCCC CTACACGCAC CATGGGTCTG GAAAACCGCC TGAATTCCTT TATTCAGGTA
    GATCCTCCAG ACCAAAAAGG AACTGAGACA AAACCGGGAC ACAGTAATAA CCCTGTCCCT
    GCATCCGAGA CTAGTGAAAG TTCTATAGCC GAGCGTACCC CGTTTAGTTC TAGCAGCTCA
    AGCAAGCACA GTTCACCAAG TGGGACTGTC GCCGCCAGGG TGAGTCCTTT TAATTACAAC
    CCAAGCCCAA GGAAGAGCAG CACAGATGGC ACTTCAGCCC GACCGTCTCA GATCCCAACG
    CCAGTGAGCA ACAACACAAA GAAACGCGAC TCAAAACCCG ACAGCACAGA ACCCAGCGGG
    ACTCAAAGTC CTAAACGCCA TTCTGGGTCT TACCTTGTGA CATCTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853412.1
    CDS147..7397
    Translation

    Target ORF information:

    RefSeq Version XM_024997644.1
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAAGCTTT CGTTTGATGA AGAGCATAGA CATGCCATGA ATGAGCTTGG TAGGAAGGCT 
    ACCCGGGGCA TTTCATCCCA GGAGCTAGGG CAGGGGCTTT CAGGGGGACT ACAGGCCATT
    GCAGAATTAT TGCAAGTGGA CTGTGAAATG TATGGGCTTA CTAATGACCA CTACAGTATT
    ACTTTAAGAC GATATGCAGG AATGGCTTTG ACAAACTTGA CTTTCGGAGA TGTAGCCAAC
    AAGGCTACTC TCTGCTCTAT GAAAGGCTGC ATGAGAGCGC TCGTGGCTCA GCTCCAGTCT
    GAAAGCGAGG ACTTGCAGCA GGTTATTGCG AGTGTTTTGA GGAATCTGTC TTGGAGAGCA
    GATGTAAATA GTAAAAAGAC TTTGCGTGAA GTTGGAAGTG TGAAAGCATT GATGGAATGT
    GCTTTGGAAG TGAAAAAGGA ATCAACCCTC AAAAGTGTAT TGAGTGCCTT ATGGAACTTG
    TCAGCACACT GCACTGAGAA TAAAGCTGAC ATATGTGCTG TGGATGGTGC GCTTGCATTT
    TTGGTTGGCA CTCTCACTTA TCGCAGCCAG ACCAATACTT TAGCCATTAT TGAAAGTGGA
    GGTGGTATAT TACGGAATGT ATCCAGCTTG ATAGCTACAA ATGAGGACCA CAGGCAAATC
    CTAAGAGAGA ATAATTGCTT ACAGACCTTA TTACAACACT TGAAATCTCA CAGTTTGACA
    ATAGTCAGCA ATGCATGCGG AACCTTGTGG AATCTCTCAG CAAGAAACCC TAAAGACCAG
    GAAGCATTGT GGGACATGGG AGCAGTCAGC ATGCTCAAGA ACCTCATTCA TTCAAAGCAC
    AAGATGATTG CTATGGGAAG TGCTGCAGCT TTAAGGAATC TCATGGCAAA TAGACCTGCA
    AAGTATAAAG ATGCCAATAT CATGTCTCCT GGTTCAAGTT TGCCTTCTCT ACATGTCAGG
    AAACAAAAGG CCCTGGAAGC AGAATTAGAT GCTCAGCATT TATCAGAAAC TTTTGACAAT
    ATTGACAATT TAAGTCCCAA GGCATCTCAT CGTAGCAAGC AGAGACACAA GCAAAATCTC
    TATGGTGACT ATGTTTTTGA CACCAATCGA CATGATGATA ACAGGTCAGA CAATTTTAAT
    ACTGGAAACA TGACTGTCCT ATCACCGTAC TTAAACACTA CAGTATTGCC CAGCTCTTCT
    TCATCAAGGG GAAGTTTAGA TAGCTCTCGT TCTGAGAAAG ATAGAAGTCT GGAGAGAGAA
    CGAGGTATTA GCCTAGGCAA CTATCACCCA GCAACAGAAA ACCCAGGAAC CTCTTCAAAG
    CGAGGTTTGC AGATTTCTAC CACTGCAGCC CAGATTGCCA AAGTCATGGA AGAAGTATCA
    GCTATTCATA CCTCTCAGGA AGACAGAAGT TCTGGGTCTA CCACAGAACT ACACTGTGGG
    ACAGATGAGA GGAATGCACT AAGAAGAAGC TCTACCACCC ACACACATGC AAACACGTAC
    AACTTTACCA AGTCAGAAAA CTCAAACAGA ACATGTCCAA TACCATATGC CAAAGTAGAA
    TATAAGAGAT CTTCAAATGA TAGTTTAAAT AGTGTCAGCA GTAGTGATGG TTATGGTAAA
    AGAGGTCAGA TGAAACCTTC AATTGAATCC TATTCTGAAG ATGATGAAAG TAAATTTTGC
    AGCTATGGTC AGTATCCAGC TGACCTAGCC CATAAAATAC ATAGTGCAAA TCATATGGAT
    GATAATGATG GAGAACTAGA TACACCAATA AATTACAGTC TCAAATATTC CGATGAACAG
    TTGAACTCTG GGAGGCAGAG CCCTTCACAG AATGAAAGAT GGGCAAGACC CAAACATATA
    CTAGAAGATG AAATAAAACC GAATGAGCAG AGACAATCAA GGAGTCAAAG CACAGCTTAT
    CCCGTGTATC CTGAGAGCAC TGATGATAAA CACCTCAAGT TCCAACCACA CTTTGGGCAG
    CAAGAATGTG TTTCCCCATA CAGGTCAAGA GCAGCCAATG GATCTGAAAC AAATCGAGTA
    GGCTCTAATC ATGGAATTAG TCAAAATGTG AACCAGTCTT TGTGTCAAGA AGATGACTAT
    GAAGACGATA AACCAACCAA CTATAGCGAA CGTTACTCTG AGGAAGGGCA GCATGAGGAA
    GAAGAGAGGC CAACCAATTA TAGCATAAAA TACAGTGAAG AAAAACATCA CGTGGATCAG
    CCTATTGATT ATAGTTTAAA ATACACCACA GACATTCCTT CTTCACAGAA ACCAGCATTT
    TCATTCTCAA AGAATTCATC TGGACAGAGC ACAAAAACTG AACACATCTC TTCAAGCAGC
    GAGAATACAT CCACAACTTC ATCTAATGCC AAGAGGCAGA ATCAGCTGCA TCCAAGCTCA
    GCACAGAGCA GAAGTGGTCA GACCCCAAAA GCCACCTCTT CCTCTTGCAA AGTCCCCTCT
    ATCAACCAAG AAACAATACA GACTTACTGT GTAGAAGATA CCCCAATATG TTTTTCAAGA
    TGCAGTTCAT TATCATCTCT GTCATCTGCT GAAGATGAAG TAGGGTGTGA TCAGACAACA
    CAAGAAGCAG AGTCTGCTAA CACTCTGCAA ATAGCTGAAA TCAAGGACAA CAGCGGACCT
    AGGTCAAATG AAGATTCTGT GAGTAAAGTT CCAGCAGGGT CACAGCACAT TAGAACCAAA
    TCCAGCAGAC TCCAGGCTTC TGGTCTGTCT TCAGAGTCAG CCAGGCACAA AGCTGTTGAA
    TTTTCTTCAG GGGCCAAATC TCCTTCCAAG AGCGGTGCTC AGACACCCAA AAGTCCACCA
    GAGCACTACG TTCAGGAGAC CCCACTCATG TTTAGCAGAT GTACTTCAGT CAGTTCACTC
    GACAGTTTTG AGAGTCGCTC GATTGCCAGC TCTGTTCAGA GTGAACCCTG CAGTGGAATG
    GTAAGTGGCA TCATAAGCCC CAGTGACCTC CCAGATAGCC CTGGACAAAC CATGCCACCA
    AGCAGAAGCA AAACCCCTCC GCCACCGCCT CCGCCTCCTC CTCAGACAGT TCAAACGAAG
    CAGGAAGTAC CTAAAAATAA AGCACCTAGT GCTGAAAAGA GAGAAAGTGG ACCCAAGCAA
    GCTGCTGTAA ATGCTGCAGT ACAAAGGGTC CAGGTTCTTC CAGAGGCTGA TACTCTGTTA
    CATTTTGCCA CAGAGAGTAC TCCTGATGGA TTTTCTTGTT CATCTAGCCT GAGCGCTCTG
    AGCCTTGATG AGCCATTTAT TCAGAAAGAT GTGGAACTAA GAATAATGCC TCCAGTTCAG
    GAAAATGACA ACGGGAATGA AACAGAAAGT GAGCAGCCTG AAGAATCAAA TGAAAACCAG
    GAAAAAGAGG CAGAAAAACC CACTGATTCT GAAAAAGATC TTTTAGATGA GTCAGATGAT
    GATGATATTG AAATACTAGA AGAGTGTATA ATTTCTGCCA TGCCAACAAA ATCTTCACGC
    AAAGCCAAAA AACCAGCCCA GACTACTTCA AAATTACCTC CACCTGTGGC AAGGAAACCA
    AGTCAGCTCC CTGTATACAA ACTTCTGCCA TCGCAAAACA GGTTACAGGC ACAAAAGCAT
    GTTAGTTTTA CACCAGGAGA TGATATGCCA CGGGTGTATT GTGTAGAAGG GACACCTATA
    AACTTTTCCA CAGCTACATC TCTGAGTGAT CTAACGATAG AATCCCCTCC AAATGAGTTA
    GCTGCTGGAG AAGGGGTTAG AGCAGGGGCA CAGTCAAGTG AGTTTGAAAA ACGAGATACC
    ATTCCTACTG AAGGCAGAAG TACAGATGAG GCTCAACGAG GGAAAGCCTC ATCTGTCACT
    GTGCCTGAAC TGGACGACAG CAAAACAGAA GAAGGTGATA TTCTTGCAGA ATGCATTAAT
    TCTGCCATGC CCAAAGGAAA AAGTCACAAG CCTTTCCGTG TGAAAAAGAT CATGGACCAG
    GTCCAGCAAG CATCTATGTC TTCATCTGGA ACTAACAAAA ATCAATTAGA TGGTAAGACG
    AAGAAACCTA CTTCACCAGT AAAACCTATA CCACAAAATA CTGAATACAG GACACGTGTA
    AGAAAAAATA CAGACTCAAA AAATAATCTA AATGCAGAAA GAAATTTCTC GGAAAACAAA
    GACTCAAAGA AACAGCACTT GAAAAATAAT TCCAAGGACT TCAATGATAA ACTGCCAAAT
    AATGAAGACA GAGTCAGAGG AAGTTTTACT TTTGATTCAC CCCATCATTA CACGCCCATT
    GAAGGCACTC CGTACTGTTT TTCACGAAAT GACTCTCTGA GTTCTCTAGA TTTTGATGAT
    GATGATGTTG ACCTTTCCAG GGAAAAGGCT GAATTAAGAA AGGGGAAGGA AAATAAAGAA
    TCAGAAGCTA AAGTGACCAA CCACACAGAA CTAACCTCAA ACCAACAATC AGCTAGTAAG
    ACACCAGCTG TTACAAAGCA GCCAATAAAT AGAGGTCAGT CTAAACCCGT GCTGCAGAAG
    CAGTCCACTT TTCCCCAGTC TTCCAAAGAT ATACCAGACA GAGGGGCAGC AACAGATGAG
    AAATTACAGA ATTTCGCTAT TGAAAATACT CCAGTTTGCT TTTCTCGAAA TTCCTCTCTA
    AGCTCTCTTA GTGACATTGA TCAAGAAAAC AACAACAACA AGGAAAATGA ACCTGTCAAA
    GAGACAGAGC CCCCTGCCTC ACAGGGAGAA CCAGGTAAAC CCCAGGCCTC AGGTTATGCT
    CCTAAATCGT TTCACGTGGA AGACACCCCT GTTTGTTTCT CAAGAAACAG TTCTCTCAGT
    TCTCTTAGTA TTGATTCTGA AGATGACCTG CTGCAGGAAT GTATAAGTTC TGCAATGCCA
    AAAAAGAAAA AGCCTTCAAG GCTCAAGCCC GATAATGAAA AGCATAGTCC CAGAAATATG
    GGTGGCATAT TAGCAGAAGA TTTGACACTC GATTTGAAAG ATATACAGAG ACCAGATTCA
    GAACATGGTT TATCCCCAGA TTCAGAAAAT TTTGATTGGA AAGCTATTCA GGAAGGTGCA
    AATTCCATAG TAAGTAGTTT ACATCAAGCT GCTGCCGCTG CATGTTTATC TCGACAAGCT
    TCATCTGATT CAGATTCTAT CCTTTCATTG AAATCGGGTA TCTCTCTGGG ATCACCATTT
    CACCTTACAC CTGATCAAGA AGAAAAACCC TTTACGAGTA ATAAAGGTCC ACGAATTCTA
    AAACCTGGGG AGAAAAGTAC ATTGGAAACT AAAAAAATAG AATCTGAAAA TAAAGGAATA
    AAAGGAGGCA AAAAGGTTTA TAAAAGTTTG ATTACTGGAA AAGTTCGATC TAATTCGGAA
    ATTTCGAGCC AAATGAAACA ACCCCTTCAA ACAAACATGC CTTCAATCTC TCGAGGTAGG
    ACAATGATTC ATATTCCAGG AGTTCGGAAT AGCTCTTCAA GTACAAGCCC AGTTTCTAAA
    AAAGGCCCGC CCCTCAAGAC TCCAGCCTCC AAAAGCCCTA GTGAAGGTCA GCCGGCTACC
    ACCTCTCCCA GAGGAACCAA GCCATCAGTG AAGTCAGAAT TAAGCCCTGT TACAAGGCAG
    GCATCCCAGA CAGCAGGATC AAACAAAGGA CCTTCTAGAT CAGGATCTAG AGATTCCACT
    CCTTCAAGAC CTGCCCAGCA ACCATTAAGT AGACCAATGC AGTCTCCAGG GCGAAACTCA
    ATCTCTCCTG GTAGAAATGG AATAAGTCCC CCTAACAAAT TATCTCAACT ACCAAGGACG
    TCATCCCCTA GTACTGCTTC AACTAAGTCC TCAGGTTCTG GGAAAATGTC TTACACATCT
    CCTGGCAGAC AGATGAGCCA GCAGAACCTC ACCAAACAAA CAGGCTTATC CAAGAATGGC
    AGTGGTATCC CAAGAAGTGA ATCTGCCTCC AAAGGGCTAA ATCAAATGAG TAATAGTAAT
    GGATCCAATA AAAAAGTAGA ACTTTCTAGA ATGTCTTCAA CAAAGTCAAG TGGAAGTGAA
    TCCGATAGGT CAGAGAGACC TGTATTAGTA CGCCAATCAA CTTTCATCAA AGAAGCTCCA
    AGCCCAACCC TAAGGAGAAA ACTGGAGGAA TCTGCTTCAT TTGAATCTCT TTCTCCATCT
    TCCAGACCCG ATTCTCCCAC ACGGTCCCAG GCTCATACTC CAGTTTTAAG TCCTTCCCTT
    CCTGATATGT CTCTATCTAC ACATTCGTCT CTTCAGTCTG GTGGATGGCG AAAACTCCCA
    CCTAACCTCA GTCCCACCAT AGAGTATAAT GATGGAAGAC CAGTAAAGCG CCATGATATA
    GCACGCTCTC ATTCCGAAAG TCCTTCCAGA CTTCCCATCA ATAGGTCAGG GACCTGGAAA
    CGTGAGCACA GCAAACACTC ATCATCACTT CCTCGAGTAA GCACTTGGAG AAGAACTGGA
    AGTTCATCCT CAATTCTTTC TGCTTCATCA GAATCTAGTG AAAAGGCAAA AAGTGAGGAC
    GAAAAACAAG TGAACTCTAT TTCAGGAAGC AAACAAACTA AAGAAAACCA GGTATCCACA
    AAAGGAACAT GGAGAAAAAT AAAAGAAAGT GAAATTTCTC CCACAAATAG TACTTCTCAG
    ACCACTTCTT CAGGTGCTGC AAATGGTGCT GAATCAAAGA CTCTGATTTA TCAAATGGCA
    CCTGCTGTTT CTAAAACAGA GGATGTTTGG GTGAGAATTG AGGATTGCCC CATTAACAAC
    CCTAGATCTG GAAGATCTCC AACAGGAAAT ACTCCCCCCG TGATTGACAC TGTTTCAGAA
    AAGGGAAACC CGAACCCTAA AGATTCAAAA GATAATCAGG GAAAACAAAA TGTGAGCAAT
    GGTAGTGCCC CTACACGCAC CATGGGTCTG GAAAACCGCC TGAATTCCTT TATTCAGGTA
    GATCCTCCAG ACCAAAAAGG AACTGAGACA AAACCGGGAC ACAGTAATAA CCCTGTCCCT
    GCATCCGAGA CTAGTGAAAG TTCTATAGCC GAGCGTACCC CGTTTAGTTC TAGCAGCTCA
    AGCAAGCACA GTTCACCAAG TGGGACTGTC GCCGCCAGGG TGAGTCCTTT TAATTACAAC
    CCAAGCCCAA GGAAGAGCAG CACAGATGGC ACTTCAGCCC GACCGTCTCA GATCCCAACG
    CCAGTGAGCA ACAACACAAA GAAACGCGAC TCAAAACCCG ACAGCACAGA ACCCAGCGGG
    ACTCAAAGTC CTAAACGCCA TTCTGGGTCT TACCTTGTGA CATCTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219625
    Clone ID Related Accession (Same CDS sequence) XM_024997645.1
    Accession Version XM_024997645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAAGCTTT CGTTTGATGA AGAGCATAGA CATGCCATGA ATGAGCTTGG GGGACTACAG 
    GCCATTGCAG AATTATTGCA AGTGGACTGT GAAATGTATG GGCTTACTAA TGACCACTAC
    AGTATTACTT TAAGACGATA TGCAGGAATG GCTTTGACAA ACTTGACTTT CGGAGATGTA
    GCCAACAAGG CTACTCTCTG CTCTATGAAA GGCTGCATGA GAGCGCTCGT GGCTCAGCTC
    CAGTCTGAAA GCGAGGACTT GCAGCAGGTT ATTGCGAGTG TTTTGAGGAA TCTGTCTTGG
    AGAGCAGATG TAAATAGTAA AAAGACTTTG CGTGAAGTTG GAAGTGTGAA AGCATTGATG
    GAATGTGCTT TGGAAGTGAA AAAGGAATCA ACCCTCAAAA GTGTATTGAG TGCCTTATGG
    AACTTGTCAG CACACTGCAC TGAGAATAAA GCTGACATAT GTGCTGTGGA TGGTGCGCTT
    GCATTTTTGG TTGGCACTCT CACTTATCGC AGCCAGACCA ATACTTTAGC CATTATTGAA
    AGTGGAGGTG GTATATTACG GAATGTATCC AGCTTGATAG CTACAAATGA GGACCACAGG
    CAAATCCTAA GAGAGAATAA TTGCTTACAG ACCTTATTAC AACACTTGAA ATCTCACAGT
    TTGACAATAG TCAGCAATGC ATGCGGAACC TTGTGGAATC TCTCAGCAAG AAACCCTAAA
    GACCAGGAAG CATTGTGGGA CATGGGAGCA GTCAGCATGC TCAAGAACCT CATTCATTCA
    AAGCACAAGA TGATTGCTAT GGGAAGTGCT GCAGCTTTAA GGAATCTCAT GGCAAATAGA
    CCTGCAAAGT ATAAAGATGC CAATATCATG TCTCCTGGTT CAAGTTTGCC TTCTCTACAT
    GTCAGGAAAC AAAAGGCCCT GGAAGCAGAA TTAGATGCTC AGCATTTATC AGAAACTTTT
    GACAATATTG ACAATTTAAG TCCCAAGGCA TCTCATCGTA GCAAGCAGAG ACACAAGCAA
    AATCTCTATG GTGACTATGT TTTTGACACC AATCGACATG ATGATAACAG GTCAGACAAT
    TTTAATACTG GAAACATGAC TGTCCTATCA CCGTACTTAA ACACTACAGT ATTGCCCAGC
    TCTTCTTCAT CAAGGGGAAG TTTAGATAGC TCTCGTTCTG AGAAAGATAG AAGTCTGGAG
    AGAGAACGAG GTATTAGCCT AGGCAACTAT CACCCAGCAA CAGAAAACCC AGGAACCTCT
    TCAAAGCGAG GTTTGCAGAT TTCTACCACT GCAGCCCAGA TTGCCAAAGT CATGGAAGAA
    GTATCAGCTA TTCATACCTC TCAGGAAGAC AGAAGTTCTG GGTCTACCAC AGAACTACAC
    TGTGGGACAG ATGAGAGGAA TGCACTAAGA AGAAGCTCTA CCACCCACAC ACATGCAAAC
    ACGTACAACT TTACCAAGTC AGAAAACTCA AACAGAACAT GTCCAATACC ATATGCCAAA
    GTAGAATATA AGAGATCTTC AAATGATAGT TTAAATAGTG TCAGCAGTAG TGATGGTTAT
    GGTAAAAGAG GTCAGATGAA ACCTTCAATT GAATCCTATT CTGAAGATGA TGAAAGTAAA
    TTTTGCAGCT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AAATACATAG TGCAAATCAT
    ATGGATGATA ATGATGGAGA ACTAGATACA CCAATAAATT ACAGTCTCAA ATATTCCGAT
    GAACAGTTGA ACTCTGGGAG GCAGAGCCCT TCACAGAATG AAAGATGGGC AAGACCCAAA
    CATATACTAG AAGATGAAAT AAAACCGAAT GAGCAGAGAC AATCAAGGAG TCAAAGCACA
    GCTTATCCCG TGTATCCTGA GAGCACTGAT GATAAACACC TCAAGTTCCA ACCACACTTT
    GGGCAGCAAG AATGTGTTTC CCCATACAGG TCAAGAGCAG CCAATGGATC TGAAACAAAT
    CGAGTAGGCT CTAATCATGG AATTAGTCAA AATGTGAACC AGTCTTTGTG TCAAGAAGAT
    GACTATGAAG ACGATAAACC AACCAACTAT AGCGAACGTT ACTCTGAGGA AGGGCAGCAT
    GAGGAAGAAG AGAGGCCAAC CAATTATAGC ATAAAATACA GTGAAGAAAA ACATCACGTG
    GATCAGCCTA TTGATTATAG TTTAAAATAC ACCACAGACA TTCCTTCTTC ACAGAAACCA
    GCATTTTCAT TCTCAAAGAA TTCATCTGGA CAGAGCACAA AAACTGAACA CATCTCTTCA
    AGCAGCGAGA ATACATCCAC AACTTCATCT AATGCCAAGA GGCAGAATCA GCTGCATCCA
    AGCTCAGCAC AGAGCAGAAG TGGTCAGACC CCAAAAGCCA CCTCTTCCTC TTGCAAAGTC
    CCCTCTATCA ACCAAGAAAC AATACAGACT TACTGTGTAG AAGATACCCC AATATGTTTT
    TCAAGATGCA GTTCATTATC ATCTCTGTCA TCTGCTGAAG ATGAAGTAGG GTGTGATCAG
    ACAACACAAG AAGCAGAGTC TGCTAACACT CTGCAAATAG CTGAAATCAA GGACAACAGC
    GGACCTAGGT CAAATGAAGA TTCTGTGAGT AAAGTTCCAG CAGGGTCACA GCACATTAGA
    ACCAAATCCA GCAGACTCCA GGCTTCTGGT CTGTCTTCAG AGTCAGCCAG GCACAAAGCT
    GTTGAATTTT CTTCAGGGGC CAAATCTCCT TCCAAGAGCG GTGCTCAGAC ACCCAAAAGT
    CCACCAGAGC ACTACGTTCA GGAGACCCCA CTCATGTTTA GCAGATGTAC TTCAGTCAGT
    TCACTCGACA GTTTTGAGAG TCGCTCGATT GCCAGCTCTG TTCAGAGTGA ACCCTGCAGT
    GGAATGGTAA GTGGCATCAT AAGCCCCAGT GACCTCCCAG ATAGCCCTGG ACAAACCATG
    CCACCAAGCA GAAGCAAAAC CCCTCCGCCA CCGCCTCCGC CTCCTCCTCA GACAGTTCAA
    ACGAAGCAGG AAGTACCTAA AAATAAAGCA CCTAGTGCTG AAAAGAGAGA AAGTGGACCC
    AAGCAAGCTG CTGTAAATGC TGCAGTACAA AGGGTCCAGG TTCTTCCAGA GGCTGATACT
    CTGTTACATT TTGCCACAGA GAGTACTCCT GATGGATTTT CTTGTTCATC TAGCCTGAGC
    GCTCTGAGCC TTGATGAGCC ATTTATTCAG AAAGATGTGG AACTAAGAAT AATGCCTCCA
    GTTCAGGAAA ATGACAACGG GAATGAAACA GAAAGTGAGC AGCCTGAAGA ATCAAATGAA
    AACCAGGAAA AAGAGGCAGA AAAACCCACT GATTCTGAAA AAGATCTTTT AGATGAGTCA
    GATGATGATG ATATTGAAAT ACTAGAAGAG TGTATAATTT CTGCCATGCC AACAAAATCT
    TCACGCAAAG CCAAAAAACC AGCCCAGACT ACTTCAAAAT TACCTCCACC TGTGGCAAGG
    AAACCAAGTC AGCTCCCTGT ATACAAACTT CTGCCATCGC AAAACAGGTT ACAGGCACAA
    AAGCATGTTA GTTTTACACC AGGAGATGAT ATGCCACGGG TGTATTGTGT AGAAGGGACA
    CCTATAAACT TTTCCACAGC TACATCTCTG AGTGATCTAA CGATAGAATC CCCTCCAAAT
    GAGTTAGCTG CTGGAGAAGG GGTTAGAGCA GGGGCACAGT CAAGTGAGTT TGAAAAACGA
    GATACCATTC CTACTGAAGG CAGAAGTACA GATGAGGCTC AACGAGGGAA AGCCTCATCT
    GTCACTGTGC CTGAACTGGA CGACAGCAAA ACAGAAGAAG GTGATATTCT TGCAGAATGC
    ATTAATTCTG CCATGCCCAA AGGAAAAAGT CACAAGCCTT TCCGTGTGAA AAAGATCATG
    GACCAGGTCC AGCAAGCATC TATGTCTTCA TCTGGAACTA ACAAAAATCA ATTAGATGGT
    AAGACGAAGA AACCTACTTC ACCAGTAAAA CCTATACCAC AAAATACTGA ATACAGGACA
    CGTGTAAGAA AAAATACAGA CTCAAAAAAT AATCTAAATG CAGAAAGAAA TTTCTCGGAA
    AACAAAGACT CAAAGAAACA GCACTTGAAA AATAATTCCA AGGACTTCAA TGATAAACTG
    CCAAATAATG AAGACAGAGT CAGAGGAAGT TTTACTTTTG ATTCACCCCA TCATTACACG
    CCCATTGAAG GCACTCCGTA CTGTTTTTCA CGAAATGACT CTCTGAGTTC TCTAGATTTT
    GATGATGATG ATGTTGACCT TTCCAGGGAA AAGGCTGAAT TAAGAAAGGG GAAGGAAAAT
    AAAGAATCAG AAGCTAAAGT GACCAACCAC ACAGAACTAA CCTCAAACCA ACAATCAGCT
    AGTAAGACAC CAGCTGTTAC AAAGCAGCCA ATAAATAGAG GTCAGTCTAA ACCCGTGCTG
    CAGAAGCAGT CCACTTTTCC CCAGTCTTCC AAAGATATAC CAGACAGAGG GGCAGCAACA
    GATGAGAAAT TACAGAATTT CGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC
    TCTCTAAGCT CTCTTAGTGA CATTGATCAA GAAAACAACA ACAACAAGGA AAATGAACCT
    GTCAAAGAGA CAGAGCCCCC TGCCTCACAG GGAGAACCAG GTAAACCCCA GGCCTCAGGT
    TATGCTCCTA AATCGTTTCA CGTGGAAGAC ACCCCTGTTT GTTTCTCAAG AAACAGTTCT
    CTCAGTTCTC TTAGTATTGA TTCTGAAGAT GACCTGCTGC AGGAATGTAT AAGTTCTGCA
    ATGCCAAAAA AGAAAAAGCC TTCAAGGCTC AAGCCCGATA ATGAAAAGCA TAGTCCCAGA
    AATATGGGTG GCATATTAGC AGAAGATTTG ACACTCGATT TGAAAGATAT ACAGAGACCA
    GATTCAGAAC ATGGTTTATC CCCAGATTCA GAAAATTTTG ATTGGAAAGC TATTCAGGAA
    GGTGCAAATT CCATAGTAAG TAGTTTACAT CAAGCTGCTG CCGCTGCATG TTTATCTCGA
    CAAGCTTCAT CTGATTCAGA TTCTATCCTT TCATTGAAAT CGGGTATCTC TCTGGGATCA
    CCATTTCACC TTACACCTGA TCAAGAAGAA AAACCCTTTA CGAGTAATAA AGGTCCACGA
    ATTCTAAAAC CTGGGGAGAA AAGTACATTG GAAACTAAAA AAATAGAATC TGAAAATAAA
    GGAATAAAAG GAGGCAAAAA GGTTTATAAA AGTTTGATTA CTGGAAAAGT TCGATCTAAT
    TCGGAAATTT CGAGCCAAAT GAAACAACCC CTTCAAACAA ACATGCCTTC AATCTCTCGA
    GGTAGGACAA TGATTCATAT TCCAGGAGTT CGGAATAGCT CTTCAAGTAC AAGCCCAGTT
    TCTAAAAAAG GCCCGCCCCT CAAGACTCCA GCCTCCAAAA GCCCTAGTGA AGGTCAGCCG
    GCTACCACCT CTCCCAGAGG AACCAAGCCA TCAGTGAAGT CAGAATTAAG CCCTGTTACA
    AGGCAGGCAT CCCAGACAGC AGGATCAAAC AAAGGACCTT CTAGATCAGG ATCTAGAGAT
    TCCACTCCTT CAAGACCTGC CCAGCAACCA TTAAGTAGAC CAATGCAGTC TCCAGGGCGA
    AACTCAATCT CTCCTGGTAG AAATGGAATA AGTCCCCCTA ACAAATTATC TCAACTACCA
    AGGACGTCAT CCCCTAGTAC TGCTTCAACT AAGTCCTCAG GTTCTGGGAA AATGTCTTAC
    ACATCTCCTG GCAGACAGAT GAGCCAGCAG AACCTCACCA AACAAACAGG CTTATCCAAG
    AATGGCAGTG GTATCCCAAG AAGTGAATCT GCCTCCAAAG GGCTAAATCA AATGAGTAAT
    AGTAATGGAT CCAATAAAAA AGTAGAACTT TCTAGAATGT CTTCAACAAA GTCAAGTGGA
    AGTGAATCCG ATAGGTCAGA GAGACCTGTA TTAGTACGCC AATCAACTTT CATCAAAGAA
    GCTCCAAGCC CAACCCTAAG GAGAAAACTG GAGGAATCTG CTTCATTTGA ATCTCTTTCT
    CCATCTTCCA GACCCGATTC TCCCACACGG TCCCAGGCTC ATACTCCAGT TTTAAGTCCT
    TCCCTTCCTG ATATGTCTCT ATCTACACAT TCGTCTCTTC AGTCTGGTGG ATGGCGAAAA
    CTCCCACCTA ACCTCAGTCC CACCATAGAG TATAATGATG GAAGACCAGT AAAGCGCCAT
    GATATAGCAC GCTCTCATTC CGAAAGTCCT TCCAGACTTC CCATCAATAG GTCAGGGACC
    TGGAAACGTG AGCACAGCAA ACACTCATCA TCACTTCCTC GAGTAAGCAC TTGGAGAAGA
    ACTGGAAGTT CATCCTCAAT TCTTTCTGCT TCATCAGAAT CTAGTGAAAA GGCAAAAAGT
    GAGGACGAAA AACAAGTGAA CTCTATTTCA GGAAGCAAAC AAACTAAAGA AAACCAGGTA
    TCCACAAAAG GAACATGGAG AAAAATAAAA GAAAGTGAAA TTTCTCCCAC AAATAGTACT
    TCTCAGACCA CTTCTTCAGG TGCTGCAAAT GGTGCTGAAT CAAAGACTCT GATTTATCAA
    ATGGCACCTG CTGTTTCTAA AACAGAGGAT GTTTGGGTGA GAATTGAGGA TTGCCCCATT
    AACAACCCTA GATCTGGAAG ATCTCCAACA GGAAATACTC CCCCCGTGAT TGACACTGTT
    TCAGAAAAGG GAAACCCGAA CCCTAAAGAT TCAAAAGATA ATCAGGGAAA ACAAAATGTG
    AGCAATGGTA GTGCCCCTAC ACGCACCATG GGTCTGGAAA ACCGCCTGAA TTCCTTTATT
    CAGGTAGATC CTCCAGACCA AAAAGGAACT GAGACAAAAC CGGGACACAG TAATAACCCT
    GTCCCTGCAT CCGAGACTAG TGAAAGTTCT ATAGCCGAGC GTACCCCGTT TAGTTCTAGC
    AGCTCAAGCA AGCACAGTTC ACCAAGTGGG ACTGTCGCCG CCAGGGTGAG TCCTTTTAAT
    TACAACCCAA GCCCAAGGAA GAGCAGCACA GATGGCACTT CAGCCCGACC GTCTCAGATC
    CCAACGCCAG TGAGCAACAA CACAAAGAAA CGCGACTCAA AACCCGACAG CACAGAACCC
    AGCGGGACTC AAAGTCCTAA ACGCCATTCT GGGTCTTACC TTGTGACATC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853413.1
    CDS147..7343
    Translation

    Target ORF information:

    RefSeq Version XM_024997645.1
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAAGCTTT CGTTTGATGA AGAGCATAGA CATGCCATGA ATGAGCTTGG GGGACTACAG 
    GCCATTGCAG AATTATTGCA AGTGGACTGT GAAATGTATG GGCTTACTAA TGACCACTAC
    AGTATTACTT TAAGACGATA TGCAGGAATG GCTTTGACAA ACTTGACTTT CGGAGATGTA
    GCCAACAAGG CTACTCTCTG CTCTATGAAA GGCTGCATGA GAGCGCTCGT GGCTCAGCTC
    CAGTCTGAAA GCGAGGACTT GCAGCAGGTT ATTGCGAGTG TTTTGAGGAA TCTGTCTTGG
    AGAGCAGATG TAAATAGTAA AAAGACTTTG CGTGAAGTTG GAAGTGTGAA AGCATTGATG
    GAATGTGCTT TGGAAGTGAA AAAGGAATCA ACCCTCAAAA GTGTATTGAG TGCCTTATGG
    AACTTGTCAG CACACTGCAC TGAGAATAAA GCTGACATAT GTGCTGTGGA TGGTGCGCTT
    GCATTTTTGG TTGGCACTCT CACTTATCGC AGCCAGACCA ATACTTTAGC CATTATTGAA
    AGTGGAGGTG GTATATTACG GAATGTATCC AGCTTGATAG CTACAAATGA GGACCACAGG
    CAAATCCTAA GAGAGAATAA TTGCTTACAG ACCTTATTAC AACACTTGAA ATCTCACAGT
    TTGACAATAG TCAGCAATGC ATGCGGAACC TTGTGGAATC TCTCAGCAAG AAACCCTAAA
    GACCAGGAAG CATTGTGGGA CATGGGAGCA GTCAGCATGC TCAAGAACCT CATTCATTCA
    AAGCACAAGA TGATTGCTAT GGGAAGTGCT GCAGCTTTAA GGAATCTCAT GGCAAATAGA
    CCTGCAAAGT ATAAAGATGC CAATATCATG TCTCCTGGTT CAAGTTTGCC TTCTCTACAT
    GTCAGGAAAC AAAAGGCCCT GGAAGCAGAA TTAGATGCTC AGCATTTATC AGAAACTTTT
    GACAATATTG ACAATTTAAG TCCCAAGGCA TCTCATCGTA GCAAGCAGAG ACACAAGCAA
    AATCTCTATG GTGACTATGT TTTTGACACC AATCGACATG ATGATAACAG GTCAGACAAT
    TTTAATACTG GAAACATGAC TGTCCTATCA CCGTACTTAA ACACTACAGT ATTGCCCAGC
    TCTTCTTCAT CAAGGGGAAG TTTAGATAGC TCTCGTTCTG AGAAAGATAG AAGTCTGGAG
    AGAGAACGAG GTATTAGCCT AGGCAACTAT CACCCAGCAA CAGAAAACCC AGGAACCTCT
    TCAAAGCGAG GTTTGCAGAT TTCTACCACT GCAGCCCAGA TTGCCAAAGT CATGGAAGAA
    GTATCAGCTA TTCATACCTC TCAGGAAGAC AGAAGTTCTG GGTCTACCAC AGAACTACAC
    TGTGGGACAG ATGAGAGGAA TGCACTAAGA AGAAGCTCTA CCACCCACAC ACATGCAAAC
    ACGTACAACT TTACCAAGTC AGAAAACTCA AACAGAACAT GTCCAATACC ATATGCCAAA
    GTAGAATATA AGAGATCTTC AAATGATAGT TTAAATAGTG TCAGCAGTAG TGATGGTTAT
    GGTAAAAGAG GTCAGATGAA ACCTTCAATT GAATCCTATT CTGAAGATGA TGAAAGTAAA
    TTTTGCAGCT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AAATACATAG TGCAAATCAT
    ATGGATGATA ATGATGGAGA ACTAGATACA CCAATAAATT ACAGTCTCAA ATATTCCGAT
    GAACAGTTGA ACTCTGGGAG GCAGAGCCCT TCACAGAATG AAAGATGGGC AAGACCCAAA
    CATATACTAG AAGATGAAAT AAAACCGAAT GAGCAGAGAC AATCAAGGAG TCAAAGCACA
    GCTTATCCCG TGTATCCTGA GAGCACTGAT GATAAACACC TCAAGTTCCA ACCACACTTT
    GGGCAGCAAG AATGTGTTTC CCCATACAGG TCAAGAGCAG CCAATGGATC TGAAACAAAT
    CGAGTAGGCT CTAATCATGG AATTAGTCAA AATGTGAACC AGTCTTTGTG TCAAGAAGAT
    GACTATGAAG ACGATAAACC AACCAACTAT AGCGAACGTT ACTCTGAGGA AGGGCAGCAT
    GAGGAAGAAG AGAGGCCAAC CAATTATAGC ATAAAATACA GTGAAGAAAA ACATCACGTG
    GATCAGCCTA TTGATTATAG TTTAAAATAC ACCACAGACA TTCCTTCTTC ACAGAAACCA
    GCATTTTCAT TCTCAAAGAA TTCATCTGGA CAGAGCACAA AAACTGAACA CATCTCTTCA
    AGCAGCGAGA ATACATCCAC AACTTCATCT AATGCCAAGA GGCAGAATCA GCTGCATCCA
    AGCTCAGCAC AGAGCAGAAG TGGTCAGACC CCAAAAGCCA CCTCTTCCTC TTGCAAAGTC
    CCCTCTATCA ACCAAGAAAC AATACAGACT TACTGTGTAG AAGATACCCC AATATGTTTT
    TCAAGATGCA GTTCATTATC ATCTCTGTCA TCTGCTGAAG ATGAAGTAGG GTGTGATCAG
    ACAACACAAG AAGCAGAGTC TGCTAACACT CTGCAAATAG CTGAAATCAA GGACAACAGC
    GGACCTAGGT CAAATGAAGA TTCTGTGAGT AAAGTTCCAG CAGGGTCACA GCACATTAGA
    ACCAAATCCA GCAGACTCCA GGCTTCTGGT CTGTCTTCAG AGTCAGCCAG GCACAAAGCT
    GTTGAATTTT CTTCAGGGGC CAAATCTCCT TCCAAGAGCG GTGCTCAGAC ACCCAAAAGT
    CCACCAGAGC ACTACGTTCA GGAGACCCCA CTCATGTTTA GCAGATGTAC TTCAGTCAGT
    TCACTCGACA GTTTTGAGAG TCGCTCGATT GCCAGCTCTG TTCAGAGTGA ACCCTGCAGT
    GGAATGGTAA GTGGCATCAT AAGCCCCAGT GACCTCCCAG ATAGCCCTGG ACAAACCATG
    CCACCAAGCA GAAGCAAAAC CCCTCCGCCA CCGCCTCCGC CTCCTCCTCA GACAGTTCAA
    ACGAAGCAGG AAGTACCTAA AAATAAAGCA CCTAGTGCTG AAAAGAGAGA AAGTGGACCC
    AAGCAAGCTG CTGTAAATGC TGCAGTACAA AGGGTCCAGG TTCTTCCAGA GGCTGATACT
    CTGTTACATT TTGCCACAGA GAGTACTCCT GATGGATTTT CTTGTTCATC TAGCCTGAGC
    GCTCTGAGCC TTGATGAGCC ATTTATTCAG AAAGATGTGG AACTAAGAAT AATGCCTCCA
    GTTCAGGAAA ATGACAACGG GAATGAAACA GAAAGTGAGC AGCCTGAAGA ATCAAATGAA
    AACCAGGAAA AAGAGGCAGA AAAACCCACT GATTCTGAAA AAGATCTTTT AGATGAGTCA
    GATGATGATG ATATTGAAAT ACTAGAAGAG TGTATAATTT CTGCCATGCC AACAAAATCT
    TCACGCAAAG CCAAAAAACC AGCCCAGACT ACTTCAAAAT TACCTCCACC TGTGGCAAGG
    AAACCAAGTC AGCTCCCTGT ATACAAACTT CTGCCATCGC AAAACAGGTT ACAGGCACAA
    AAGCATGTTA GTTTTACACC AGGAGATGAT ATGCCACGGG TGTATTGTGT AGAAGGGACA
    CCTATAAACT TTTCCACAGC TACATCTCTG AGTGATCTAA CGATAGAATC CCCTCCAAAT
    GAGTTAGCTG CTGGAGAAGG GGTTAGAGCA GGGGCACAGT CAAGTGAGTT TGAAAAACGA
    GATACCATTC CTACTGAAGG CAGAAGTACA GATGAGGCTC AACGAGGGAA AGCCTCATCT
    GTCACTGTGC CTGAACTGGA CGACAGCAAA ACAGAAGAAG GTGATATTCT TGCAGAATGC
    ATTAATTCTG CCATGCCCAA AGGAAAAAGT CACAAGCCTT TCCGTGTGAA AAAGATCATG
    GACCAGGTCC AGCAAGCATC TATGTCTTCA TCTGGAACTA ACAAAAATCA ATTAGATGGT
    AAGACGAAGA AACCTACTTC ACCAGTAAAA CCTATACCAC AAAATACTGA ATACAGGACA
    CGTGTAAGAA AAAATACAGA CTCAAAAAAT AATCTAAATG CAGAAAGAAA TTTCTCGGAA
    AACAAAGACT CAAAGAAACA GCACTTGAAA AATAATTCCA AGGACTTCAA TGATAAACTG
    CCAAATAATG AAGACAGAGT CAGAGGAAGT TTTACTTTTG ATTCACCCCA TCATTACACG
    CCCATTGAAG GCACTCCGTA CTGTTTTTCA CGAAATGACT CTCTGAGTTC TCTAGATTTT
    GATGATGATG ATGTTGACCT TTCCAGGGAA AAGGCTGAAT TAAGAAAGGG GAAGGAAAAT
    AAAGAATCAG AAGCTAAAGT GACCAACCAC ACAGAACTAA CCTCAAACCA ACAATCAGCT
    AGTAAGACAC CAGCTGTTAC AAAGCAGCCA ATAAATAGAG GTCAGTCTAA ACCCGTGCTG
    CAGAAGCAGT CCACTTTTCC CCAGTCTTCC AAAGATATAC CAGACAGAGG GGCAGCAACA
    GATGAGAAAT TACAGAATTT CGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC
    TCTCTAAGCT CTCTTAGTGA CATTGATCAA GAAAACAACA ACAACAAGGA AAATGAACCT
    GTCAAAGAGA CAGAGCCCCC TGCCTCACAG GGAGAACCAG GTAAACCCCA GGCCTCAGGT
    TATGCTCCTA AATCGTTTCA CGTGGAAGAC ACCCCTGTTT GTTTCTCAAG AAACAGTTCT
    CTCAGTTCTC TTAGTATTGA TTCTGAAGAT GACCTGCTGC AGGAATGTAT AAGTTCTGCA
    ATGCCAAAAA AGAAAAAGCC TTCAAGGCTC AAGCCCGATA ATGAAAAGCA TAGTCCCAGA
    AATATGGGTG GCATATTAGC AGAAGATTTG ACACTCGATT TGAAAGATAT ACAGAGACCA
    GATTCAGAAC ATGGTTTATC CCCAGATTCA GAAAATTTTG ATTGGAAAGC TATTCAGGAA
    GGTGCAAATT CCATAGTAAG TAGTTTACAT CAAGCTGCTG CCGCTGCATG TTTATCTCGA
    CAAGCTTCAT CTGATTCAGA TTCTATCCTT TCATTGAAAT CGGGTATCTC TCTGGGATCA
    CCATTTCACC TTACACCTGA TCAAGAAGAA AAACCCTTTA CGAGTAATAA AGGTCCACGA
    ATTCTAAAAC CTGGGGAGAA AAGTACATTG GAAACTAAAA AAATAGAATC TGAAAATAAA
    GGAATAAAAG GAGGCAAAAA GGTTTATAAA AGTTTGATTA CTGGAAAAGT TCGATCTAAT
    TCGGAAATTT CGAGCCAAAT GAAACAACCC CTTCAAACAA ACATGCCTTC AATCTCTCGA
    GGTAGGACAA TGATTCATAT TCCAGGAGTT CGGAATAGCT CTTCAAGTAC AAGCCCAGTT
    TCTAAAAAAG GCCCGCCCCT CAAGACTCCA GCCTCCAAAA GCCCTAGTGA AGGTCAGCCG
    GCTACCACCT CTCCCAGAGG AACCAAGCCA TCAGTGAAGT CAGAATTAAG CCCTGTTACA
    AGGCAGGCAT CCCAGACAGC AGGATCAAAC AAAGGACCTT CTAGATCAGG ATCTAGAGAT
    TCCACTCCTT CAAGACCTGC CCAGCAACCA TTAAGTAGAC CAATGCAGTC TCCAGGGCGA
    AACTCAATCT CTCCTGGTAG AAATGGAATA AGTCCCCCTA ACAAATTATC TCAACTACCA
    AGGACGTCAT CCCCTAGTAC TGCTTCAACT AAGTCCTCAG GTTCTGGGAA AATGTCTTAC
    ACATCTCCTG GCAGACAGAT GAGCCAGCAG AACCTCACCA AACAAACAGG CTTATCCAAG
    AATGGCAGTG GTATCCCAAG AAGTGAATCT GCCTCCAAAG GGCTAAATCA AATGAGTAAT
    AGTAATGGAT CCAATAAAAA AGTAGAACTT TCTAGAATGT CTTCAACAAA GTCAAGTGGA
    AGTGAATCCG ATAGGTCAGA GAGACCTGTA TTAGTACGCC AATCAACTTT CATCAAAGAA
    GCTCCAAGCC CAACCCTAAG GAGAAAACTG GAGGAATCTG CTTCATTTGA ATCTCTTTCT
    CCATCTTCCA GACCCGATTC TCCCACACGG TCCCAGGCTC ATACTCCAGT TTTAAGTCCT
    TCCCTTCCTG ATATGTCTCT ATCTACACAT TCGTCTCTTC AGTCTGGTGG ATGGCGAAAA
    CTCCCACCTA ACCTCAGTCC CACCATAGAG TATAATGATG GAAGACCAGT AAAGCGCCAT
    GATATAGCAC GCTCTCATTC CGAAAGTCCT TCCAGACTTC CCATCAATAG GTCAGGGACC
    TGGAAACGTG AGCACAGCAA ACACTCATCA TCACTTCCTC GAGTAAGCAC TTGGAGAAGA
    ACTGGAAGTT CATCCTCAAT TCTTTCTGCT TCATCAGAAT CTAGTGAAAA GGCAAAAAGT
    GAGGACGAAA AACAAGTGAA CTCTATTTCA GGAAGCAAAC AAACTAAAGA AAACCAGGTA
    TCCACAAAAG GAACATGGAG AAAAATAAAA GAAAGTGAAA TTTCTCCCAC AAATAGTACT
    TCTCAGACCA CTTCTTCAGG TGCTGCAAAT GGTGCTGAAT CAAAGACTCT GATTTATCAA
    ATGGCACCTG CTGTTTCTAA AACAGAGGAT GTTTGGGTGA GAATTGAGGA TTGCCCCATT
    AACAACCCTA GATCTGGAAG ATCTCCAACA GGAAATACTC CCCCCGTGAT TGACACTGTT
    TCAGAAAAGG GAAACCCGAA CCCTAAAGAT TCAAAAGATA ATCAGGGAAA ACAAAATGTG
    AGCAATGGTA GTGCCCCTAC ACGCACCATG GGTCTGGAAA ACCGCCTGAA TTCCTTTATT
    CAGGTAGATC CTCCAGACCA AAAAGGAACT GAGACAAAAC CGGGACACAG TAATAACCCT
    GTCCCTGCAT CCGAGACTAG TGAAAGTTCT ATAGCCGAGC GTACCCCGTT TAGTTCTAGC
    AGCTCAAGCA AGCACAGTTC ACCAAGTGGG ACTGTCGCCG CCAGGGTGAG TCCTTTTAAT
    TACAACCCAA GCCCAAGGAA GAGCAGCACA GATGGCACTT CAGCCCGACC GTCTCAGATC
    CCAACGCCAG TGAGCAACAA CACAAAGAAA CGCGACTCAA AACCCGACAG CACAGAACCC
    AGCGGGACTC AAAGTCCTAA ACGCCATTCT GGGTCTTACC TTGTGACATC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39178
    Clone ID Related Accession (Same CDS sequence) NM_001075986.2
    Accession Version NM_001075986.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03000697.1 and AAFC03020263.1. On Dec 12, 2009 this sequence version replaced NM_001075986.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CTCCTCCACC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001069454.2
    CDS126..8681
    Translation

    Target ORF information:

    RefSeq Version NM_001075986.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001075986.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CTCCTCCACC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219610
    Clone ID Related Accession (Same CDS sequence) XM_015472979.2
    Accession Version XM_015472979.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGTA GGAAGGCTAC CCGGGGCATT
    TCATCCCAGG AGCTAGGGCA GGGGCTTTCA GGGGGACTAC AGGCCATTGC AGAATTATTG
    CAAGTGGACT GTGAAATGTA TGGGCTTACT AATGACCACT ACAGTATTAC TTTAAGACGA
    TATGCAGGAA TGGCTTTGAC AAACTTGACT TTCGGAGATG TAGCCAACAA GGCTACTCTC
    TGCTCTATGA AAGGCTGCAT GAGAGCGCTC GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC
    TTGCAGCAGG TTATTGCGAG TGTTTTGAGG AATCTGTCTT GGAGAGCAGA TGTAAATAGT
    AAAAAGACTT TGCGTGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTG
    AAAAAGGAAT CAACCCTCAA AAGTGTATTG AGTGCCTTAT GGAACTTGTC AGCACACTGC
    ACTGAGAATA AAGCTGACAT ATGTGCTGTG GATGGTGCGC TTGCATTTTT GGTTGGCACT
    CTCACTTATC GCAGCCAGAC CAATACTTTA GCCATTATTG AAAGTGGAGG TGGTATATTA
    CGGAATGTAT CCAGCTTGAT AGCTACAAAT GAGGACCACA GGCAAATCCT AAGAGAGAAT
    AATTGCTTAC AGACCTTATT ACAACACTTG AAATCTCACA GTTTGACAAT AGTCAGCAAT
    GCATGCGGAA CCTTGTGGAA TCTCTCAGCA AGAAACCCTA AAGACCAGGA AGCATTGTGG
    GACATGGGAG CAGTCAGCAT GCTCAAGAAC CTCATTCATT CAAAGCACAA GATGATTGCT
    ATGGGAAGTG CTGCAGCTTT AAGGAATCTC ATGGCAAATA GACCTGCAAA GTATAAAGAT
    GCCAATATCA TGTCTCCTGG TTCAAGTTTG CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC
    CTGGAAGCAG AATTAGATGC TCAGCATTTA TCAGAAACTT TTGACAATAT TGACAATTTA
    AGTCCCAAGG CATCTCATCG TAGCAAGCAG AGACACAAGC AAAATCTCTA TGGTGACTAT
    GTTTTTGACA CCAATCGACA TGATGATAAC AGGTCAGACA ATTTTAATAC TGGAAACATG
    ACTGTCCTAT CACCGTACTT AAACACTACA GTATTGCCCA GCTCTTCTTC ATCAAGGGGA
    AGTTTAGATA GCTCTCGTTC TGAGAAAGAT AGAAGTCTGG AGAGAGAACG AGGTATTAGC
    CTAGGCAACT ATCACCCAGC AACAGAAAAC CCAGGAACCT CTTCAAAGCG AGGTTTGCAG
    ATTTCTACCA CTGCAGCCCA GATTGCCAAA GTCATGGAAG AAGTATCAGC TATTCATACC
    TCTCAGGAAG ACAGAAGTTC TGGGTCTACC ACAGAACTAC ACTGTGGGAC AGATGAGAGG
    AATGCACTAA GAAGAAGCTC TACCACCCAC ACACATGCAA ACACGTACAA CTTTACCAAG
    TCAGAAAACT CAAACAGAAC ATGTCCAATA CCATATGCCA AAGTAGAATA TAAGAGATCT
    TCAAATGATA GTTTAAATAG TGTCAGCAGT AGTGATGGTT ATGGTAAAAG AGGTCAGATG
    AAACCTTCAA TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG CTATGGTCAG
    TATCCAGCTG ACCTAGCCCA TAAAATACAT AGTGCAAATC ATATGGATGA TAATGATGGA
    GAACTAGATA CACCAATAAA TTACAGTCTC AAATATTCCG ATGAACAGTT GAACTCTGGG
    AGGCAGAGCC CTTCACAGAA TGAAAGATGG GCAAGACCCA AACATATACT AGAAGATGAA
    ATAAAACCGA ATGAGCAGAG ACAATCAAGG AGTCAAAGCA CAGCTTATCC CGTGTATCCT
    GAGAGCACTG ATGATAAACA CCTCAAGTTC CAACCACACT TTGGGCAGCA AGAATGTGTT
    TCCCCATACA GGTCAAGAGC AGCCAATGGA TCTGAAACAA ATCGAGTAGG CTCTAATCAT
    GGAATTAGTC AAAATGTGAA CCAGTCTTTG TGTCAAGAAG ATGACTATGA AGACGATAAA
    CCAACCAACT ATAGCGAACG TTACTCTGAG GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA
    ACCAATTATA GCATAAAATA CAGTGAAGAA AAACATCACG TGGATCAGCC TATTGATTAT
    AGTTTAAAAT ACACCACAGA CATTCCTTCT TCACAGAAAC CAGCATTTTC ATTCTCAAAG
    AATTCATCTG GACAGAGCAC AAAAACTGAA CACATCTCTT CAAGCAGCGA GAATACATCC
    ACAACTTCAT CTAATGCCAA GAGGCAGAAT CAGCTGCATC CAAGCTCAGC ACAGAGCAGA
    AGTGGTCAGA CCCCAAAAGC CACCTCTTCC TCTTGCAAAG TCCCCTCTAT CAACCAAGAA
    ACAATACAGA CTTACTGTGT AGAAGATACC CCAATATGTT TTTCAAGATG CAGTTCATTA
    TCATCTCTGT CATCTGCTGA AGATGAAGTA GGGTGTGATC AGACAACACA AGAAGCAGAG
    TCTGCTAACA CTCTGCAAAT AGCTGAAATC AAGGACAACA GCGGACCTAG GTCAAATGAA
    GATTCTGTGA GTAAAGTTCC AGCAGGGTCA CAGCACATTA GAACCAAATC CAGCAGACTC
    CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC AGGCACAAAG CTGTTGAATT TTCTTCAGGG
    GCCAAATCTC CTTCCAAGAG CGGTGCTCAG ACACCCAAAA GTCCACCAGA GCACTACGTT
    CAGGAGACCC CACTCATGTT TAGCAGATGT ACTTCAGTCA GTTCACTCGA CAGTTTTGAG
    AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT GAACCCTGCA GTGGAATGGT AAGTGGCATC
    ATAAGCCCCA GTGACCTCCC AGATAGCCCT GGACAAACCA TGCCACCAAG CAGAAGCAAA
    ACCCCTCCGC CACCGCCTCC GCCTCCTCCT CAGACAGTTC AAACGAAGCA GGAAGTACCT
    AAAAATAAAG CACCTAGTGC TGAAAAGAGA GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT
    GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA GAGGCTGATA CTCTGTTACA TTTTGCCACA
    GAGAGTACTC CTGATGGATT TTCTTGTTCA TCTAGCCTGA GCGCTCTGAG CCTTGATGAG
    CCATTTATTC AGAAAGATGT GGAACTAAGA ATAATGCCTC CAGTTCAGGA AAATGACAAC
    GGGAATGAAA CAGAAAGTGA GCAGCCTGAA GAATCAAATG AAAACCAGGA AAAAGAGGCA
    GAAAAACCCA CTGATTCTGA AAAAGATCTT TTAGATGAGT CAGATGATGA TGATATTGAA
    ATACTAGAAG AGTGTATAAT TTCTGCCATG CCAACAAAAT CTTCACGCAA AGCCAAAAAA
    CCAGCCCAGA CTACTTCAAA ATTACCTCCA CCTGTGGCAA GGAAACCAAG TCAGCTCCCT
    GTATACAAAC TTCTGCCATC GCAAAACAGG TTACAGGCAC AAAAGCATGT TAGTTTTACA
    CCAGGAGATG ATATGCCACG GGTGTATTGT GTAGAAGGGA CACCTATAAA CTTTTCCACA
    GCTACATCTC TGAGTGATCT AACGATAGAA TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA
    GGGGTTAGAG CAGGGGCACA GTCAAGTGAG TTTGAAAAAC GAGATACCAT TCCTACTGAA
    GGCAGAAGTA CAGATGAGGC TCAACGAGGG AAAGCCTCAT CTGTCACTGT GCCTGAACTG
    GACGACAGCA AAACAGAAGA AGGTGATATT CTTGCAGAAT GCATTAATTC TGCCATGCCC
    AAAGGAAAAA GTCACAAGCC TTTCCGTGTG AAAAAGATCA TGGACCAGGT CCAGCAAGCA
    TCTATGTCTT CATCTGGAAC TAACAAAAAT CAATTAGATG GTAAGACGAA GAAACCTACT
    TCACCAGTAA AACCTATACC ACAAAATACT GAATACAGGA CACGTGTAAG AAAAAATACA
    GACTCAAAAA ATAATCTAAA TGCAGAAAGA AATTTCTCGG AAAACAAAGA CTCAAAGAAA
    CAGCACTTGA AAAATAATTC CAAGGACTTC AATGATAAAC TGCCAAATAA TGAAGACAGA
    GTCAGAGGAA GTTTTACTTT TGATTCACCC CATCATTACA CGCCCATTGA AGGCACTCCG
    TACTGTTTTT CACGAAATGA CTCTCTGAGT TCTCTAGATT TTGATGATGA TGATGTTGAC
    CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG GGGAAGGAAA ATAAAGAATC AGAAGCTAAA
    GTGACCAACC ACACAGAACT AACCTCAAAC CAACAATCAG CTAGTAAGAC ACCAGCTGTT
    ACAAAGCAGC CAATAAATAG AGGTCAGTCT AAACCCGTGC TGCAGAAGCA GTCCACTTTT
    CCCCAGTCTT CCAAAGATAT ACCAGACAGA GGGGCAGCAA CAGATGAGAA ATTACAGAAT
    TTCGCTATTG AAAATACTCC AGTTTGCTTT TCTCGAAATT CCTCTCTAAG CTCTCTTAGT
    GACATTGATC AAGAAAACAA CAACAACAAG GAAAATGAAC CTGTCAAAGA GACAGAGCCC
    CCTGCCTCAC AGGGAGAACC AGGTAAACCC CAGGCCTCAG GTTATGCTCC TAAATCGTTT
    CACGTGGAAG ACACCCCTGT TTGTTTCTCA AGAAACAGTT CTCTCAGTTC TCTTAGTATT
    GATTCTGAAG ATGACCTGCT GCAGGAATGT ATAAGTTCTG CAATGCCAAA AAAGAAAAAG
    CCTTCAAGGC TCAAGCCCGA TAATGAAAAG CATAGTCCCA GAAATATGGG TGGCATATTA
    GCAGAAGATT TGACACTCGA TTTGAAAGAT ATACAGAGAC CAGATTCAGA ACATGGTTTA
    TCCCCAGATT CAGAAAATTT TGATTGGAAA GCTATTCAGG AAGGTGCAAA TTCCATAGTA
    AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA TGTTTATCTC GACAAGCTTC ATCTGATTCA
    GATTCTATCC TTTCATTGAA ATCGGGTATC TCTCTGGGAT CACCATTTCA CCTTACACCT
    GATCAAGAAG AAAAACCCTT TACGAGTAAT AAAGGTCCAC GAATTCTAAA ACCTGGGGAG
    AAAAGTACAT TGGAAACTAA AAAAATAGAA TCTGAAAATA AAGGAATAAA AGGAGGCAAA
    AAGGTTTATA AAAGTTTGAT TACTGGAAAA GTTCGATCTA ATTCGGAAAT TTCGAGCCAA
    ATGAAACAAC CCCTTCAAAC AAACATGCCT TCAATCTCTC GAGGTAGGAC AATGATTCAT
    ATTCCAGGAG TTCGGAATAG CTCTTCAAGT ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC
    CTCAAGACTC CAGCCTCCAA AAGCCCTAGT GAAGGTCAGC CGGCTACCAC CTCTCCCAGA
    GGAACCAAGC CATCAGTGAA GTCAGAATTA AGCCCTGTTA CAAGGCAGGC ATCCCAGACA
    GCAGGATCAA ACAAAGGACC TTCTAGATCA GGATCTAGAG ATTCCACTCC TTCAAGACCT
    GCCCAGCAAC CATTAAGTAG ACCAATGCAG TCTCCAGGGC GAAACTCAAT CTCTCCTGGT
    AGAAATGGAA TAAGTCCCCC TAACAAATTA TCTCAACTAC CAAGGACGTC ATCCCCTAGT
    ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG AAAATGTCTT ACACATCTCC TGGCAGACAG
    ATGAGCCAGC AGAACCTCAC CAAACAAACA GGCTTATCCA AGAATGGCAG TGGTATCCCA
    AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT CAAATGAGTA ATAGTAATGG ATCCAATAAA
    AAAGTAGAAC TTTCTAGAAT GTCTTCAACA AAGTCAAGTG GAAGTGAATC CGATAGGTCA
    GAGAGACCTG TATTAGTACG CCAATCAACT TTCATCAAAG AAGCTCCAAG CCCAACCCTA
    AGGAGAAAAC TGGAGGAATC TGCTTCATTT GAATCTCTTT CTCCATCTTC CAGACCCGAT
    TCTCCCACAC GGTCCCAGGC TCATACTCCA GTTTTAAGTC CTTCCCTTCC TGATATGTCT
    CTATCTACAC ATTCGTCTCT TCAGTCTGGT GGATGGCGAA AACTCCCACC TAACCTCAGT
    CCCACCATAG AGTATAATGA TGGAAGACCA GTAAAGCGCC ATGATATAGC ACGCTCTCAT
    TCCGAAAGTC CTTCCAGACT TCCCATCAAT AGGTCAGGGA CCTGGAAACG TGAGCACAGC
    AAACACTCAT CATCACTTCC TCGAGTAAGC ACTTGGAGAA GAACTGGAAG TTCATCCTCA
    ATTCTTTCTG CTTCATCAGA ATCTAGTGAA AAGGCAAAAA GTGAGGACGA AAAACAAGTG
    AACTCTATTT CAGGAAGCAA ACAAACTAAA GAAAACCAGG TATCCACAAA AGGAACATGG
    AGAAAAATAA AAGAAAGTGA AATTTCTCCC ACAAATAGTA CTTCTCAGAC CACTTCTTCA
    GGTGCTGCAA ATGGTGCTGA ATCAAAGACT CTGATTTATC AAATGGCACC TGCTGTTTCT
    AAAACAGAGG ATGTTTGGGT GAGAATTGAG GATTGCCCCA TTAACAACCC TAGATCTGGA
    AGATCTCCAA CAGGAAATAC TCCCCCCGTG ATTGACACTG TTTCAGAAAA GGGAAACCCG
    AACCCTAAAG ATTCAAAAGA TAATCAGGGA AAACAAAATG TGAGCAATGG TAGTGCCCCT
    ACACGCACCA TGGGTCTGGA AAACCGCCTG AATTCCTTTA TTCAGGTAGA TCCTCCAGAC
    CAAAAAGGAA CTGAGACAAA ACCGGGACAC AGTAATAACC CTGTCCCTGC ATCCGAGACT
    AGTGAAAGTT CTATAGCCGA GCGTACCCCG TTTAGTTCTA GCAGCTCAAG CAAGCACAGT
    TCACCAAGTG GGACTGTCGC CGCCAGGGTG AGTCCTTTTA ATTACAACCC AAGCCCAAGG
    AAGAGCAGCA CAGATGGCAC TTCAGCCCGA CCGTCTCAGA TCCCAACGCC AGTGAGCAAC
    AACACAAAGA AACGCGACTC AAAACCCGAC AGCACAGAAC CCAGCGGGAC TCAAAGTCCT
    AAACGCCATT CTGGGTCTTA CCTTGTGACA TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328465.1
    CDS132..8870
    Translation

    Target ORF information:

    RefSeq Version XM_015472979.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472979.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGTA GGAAGGCTAC CCGGGGCATT
    TCATCCCAGG AGCTAGGGCA GGGGCTTTCA GGGGGACTAC AGGCCATTGC AGAATTATTG
    CAAGTGGACT GTGAAATGTA TGGGCTTACT AATGACCACT ACAGTATTAC TTTAAGACGA
    TATGCAGGAA TGGCTTTGAC AAACTTGACT TTCGGAGATG TAGCCAACAA GGCTACTCTC
    TGCTCTATGA AAGGCTGCAT GAGAGCGCTC GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC
    TTGCAGCAGG TTATTGCGAG TGTTTTGAGG AATCTGTCTT GGAGAGCAGA TGTAAATAGT
    AAAAAGACTT TGCGTGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTG
    AAAAAGGAAT CAACCCTCAA AAGTGTATTG AGTGCCTTAT GGAACTTGTC AGCACACTGC
    ACTGAGAATA AAGCTGACAT ATGTGCTGTG GATGGTGCGC TTGCATTTTT GGTTGGCACT
    CTCACTTATC GCAGCCAGAC CAATACTTTA GCCATTATTG AAAGTGGAGG TGGTATATTA
    CGGAATGTAT CCAGCTTGAT AGCTACAAAT GAGGACCACA GGCAAATCCT AAGAGAGAAT
    AATTGCTTAC AGACCTTATT ACAACACTTG AAATCTCACA GTTTGACAAT AGTCAGCAAT
    GCATGCGGAA CCTTGTGGAA TCTCTCAGCA AGAAACCCTA AAGACCAGGA AGCATTGTGG
    GACATGGGAG CAGTCAGCAT GCTCAAGAAC CTCATTCATT CAAAGCACAA GATGATTGCT
    ATGGGAAGTG CTGCAGCTTT AAGGAATCTC ATGGCAAATA GACCTGCAAA GTATAAAGAT
    GCCAATATCA TGTCTCCTGG TTCAAGTTTG CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC
    CTGGAAGCAG AATTAGATGC TCAGCATTTA TCAGAAACTT TTGACAATAT TGACAATTTA
    AGTCCCAAGG CATCTCATCG TAGCAAGCAG AGACACAAGC AAAATCTCTA TGGTGACTAT
    GTTTTTGACA CCAATCGACA TGATGATAAC AGGTCAGACA ATTTTAATAC TGGAAACATG
    ACTGTCCTAT CACCGTACTT AAACACTACA GTATTGCCCA GCTCTTCTTC ATCAAGGGGA
    AGTTTAGATA GCTCTCGTTC TGAGAAAGAT AGAAGTCTGG AGAGAGAACG AGGTATTAGC
    CTAGGCAACT ATCACCCAGC AACAGAAAAC CCAGGAACCT CTTCAAAGCG AGGTTTGCAG
    ATTTCTACCA CTGCAGCCCA GATTGCCAAA GTCATGGAAG AAGTATCAGC TATTCATACC
    TCTCAGGAAG ACAGAAGTTC TGGGTCTACC ACAGAACTAC ACTGTGGGAC AGATGAGAGG
    AATGCACTAA GAAGAAGCTC TACCACCCAC ACACATGCAA ACACGTACAA CTTTACCAAG
    TCAGAAAACT CAAACAGAAC ATGTCCAATA CCATATGCCA AAGTAGAATA TAAGAGATCT
    TCAAATGATA GTTTAAATAG TGTCAGCAGT AGTGATGGTT ATGGTAAAAG AGGTCAGATG
    AAACCTTCAA TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG CTATGGTCAG
    TATCCAGCTG ACCTAGCCCA TAAAATACAT AGTGCAAATC ATATGGATGA TAATGATGGA
    GAACTAGATA CACCAATAAA TTACAGTCTC AAATATTCCG ATGAACAGTT GAACTCTGGG
    AGGCAGAGCC CTTCACAGAA TGAAAGATGG GCAAGACCCA AACATATACT AGAAGATGAA
    ATAAAACCGA ATGAGCAGAG ACAATCAAGG AGTCAAAGCA CAGCTTATCC CGTGTATCCT
    GAGAGCACTG ATGATAAACA CCTCAAGTTC CAACCACACT TTGGGCAGCA AGAATGTGTT
    TCCCCATACA GGTCAAGAGC AGCCAATGGA TCTGAAACAA ATCGAGTAGG CTCTAATCAT
    GGAATTAGTC AAAATGTGAA CCAGTCTTTG TGTCAAGAAG ATGACTATGA AGACGATAAA
    CCAACCAACT ATAGCGAACG TTACTCTGAG GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA
    ACCAATTATA GCATAAAATA CAGTGAAGAA AAACATCACG TGGATCAGCC TATTGATTAT
    AGTTTAAAAT ACACCACAGA CATTCCTTCT TCACAGAAAC CAGCATTTTC ATTCTCAAAG
    AATTCATCTG GACAGAGCAC AAAAACTGAA CACATCTCTT CAAGCAGCGA GAATACATCC
    ACAACTTCAT CTAATGCCAA GAGGCAGAAT CAGCTGCATC CAAGCTCAGC ACAGAGCAGA
    AGTGGTCAGA CCCCAAAAGC CACCTCTTCC TCTTGCAAAG TCCCCTCTAT CAACCAAGAA
    ACAATACAGA CTTACTGTGT AGAAGATACC CCAATATGTT TTTCAAGATG CAGTTCATTA
    TCATCTCTGT CATCTGCTGA AGATGAAGTA GGGTGTGATC AGACAACACA AGAAGCAGAG
    TCTGCTAACA CTCTGCAAAT AGCTGAAATC AAGGACAACA GCGGACCTAG GTCAAATGAA
    GATTCTGTGA GTAAAGTTCC AGCAGGGTCA CAGCACATTA GAACCAAATC CAGCAGACTC
    CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC AGGCACAAAG CTGTTGAATT TTCTTCAGGG
    GCCAAATCTC CTTCCAAGAG CGGTGCTCAG ACACCCAAAA GTCCACCAGA GCACTACGTT
    CAGGAGACCC CACTCATGTT TAGCAGATGT ACTTCAGTCA GTTCACTCGA CAGTTTTGAG
    AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT GAACCCTGCA GTGGAATGGT AAGTGGCATC
    ATAAGCCCCA GTGACCTCCC AGATAGCCCT GGACAAACCA TGCCACCAAG CAGAAGCAAA
    ACCCCTCCGC CACCGCCTCC GCCTCCTCCT CAGACAGTTC AAACGAAGCA GGAAGTACCT
    AAAAATAAAG CACCTAGTGC TGAAAAGAGA GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT
    GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA GAGGCTGATA CTCTGTTACA TTTTGCCACA
    GAGAGTACTC CTGATGGATT TTCTTGTTCA TCTAGCCTGA GCGCTCTGAG CCTTGATGAG
    CCATTTATTC AGAAAGATGT GGAACTAAGA ATAATGCCTC CAGTTCAGGA AAATGACAAC
    GGGAATGAAA CAGAAAGTGA GCAGCCTGAA GAATCAAATG AAAACCAGGA AAAAGAGGCA
    GAAAAACCCA CTGATTCTGA AAAAGATCTT TTAGATGAGT CAGATGATGA TGATATTGAA
    ATACTAGAAG AGTGTATAAT TTCTGCCATG CCAACAAAAT CTTCACGCAA AGCCAAAAAA
    CCAGCCCAGA CTACTTCAAA ATTACCTCCA CCTGTGGCAA GGAAACCAAG TCAGCTCCCT
    GTATACAAAC TTCTGCCATC GCAAAACAGG TTACAGGCAC AAAAGCATGT TAGTTTTACA
    CCAGGAGATG ATATGCCACG GGTGTATTGT GTAGAAGGGA CACCTATAAA CTTTTCCACA
    GCTACATCTC TGAGTGATCT AACGATAGAA TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA
    GGGGTTAGAG CAGGGGCACA GTCAAGTGAG TTTGAAAAAC GAGATACCAT TCCTACTGAA
    GGCAGAAGTA CAGATGAGGC TCAACGAGGG AAAGCCTCAT CTGTCACTGT GCCTGAACTG
    GACGACAGCA AAACAGAAGA AGGTGATATT CTTGCAGAAT GCATTAATTC TGCCATGCCC
    AAAGGAAAAA GTCACAAGCC TTTCCGTGTG AAAAAGATCA TGGACCAGGT CCAGCAAGCA
    TCTATGTCTT CATCTGGAAC TAACAAAAAT CAATTAGATG GTAAGACGAA GAAACCTACT
    TCACCAGTAA AACCTATACC ACAAAATACT GAATACAGGA CACGTGTAAG AAAAAATACA
    GACTCAAAAA ATAATCTAAA TGCAGAAAGA AATTTCTCGG AAAACAAAGA CTCAAAGAAA
    CAGCACTTGA AAAATAATTC CAAGGACTTC AATGATAAAC TGCCAAATAA TGAAGACAGA
    GTCAGAGGAA GTTTTACTTT TGATTCACCC CATCATTACA CGCCCATTGA AGGCACTCCG
    TACTGTTTTT CACGAAATGA CTCTCTGAGT TCTCTAGATT TTGATGATGA TGATGTTGAC
    CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG GGGAAGGAAA ATAAAGAATC AGAAGCTAAA
    GTGACCAACC ACACAGAACT AACCTCAAAC CAACAATCAG CTAGTAAGAC ACCAGCTGTT
    ACAAAGCAGC CAATAAATAG AGGTCAGTCT AAACCCGTGC TGCAGAAGCA GTCCACTTTT
    CCCCAGTCTT CCAAAGATAT ACCAGACAGA GGGGCAGCAA CAGATGAGAA ATTACAGAAT
    TTCGCTATTG AAAATACTCC AGTTTGCTTT TCTCGAAATT CCTCTCTAAG CTCTCTTAGT
    GACATTGATC AAGAAAACAA CAACAACAAG GAAAATGAAC CTGTCAAAGA GACAGAGCCC
    CCTGCCTCAC AGGGAGAACC AGGTAAACCC CAGGCCTCAG GTTATGCTCC TAAATCGTTT
    CACGTGGAAG ACACCCCTGT TTGTTTCTCA AGAAACAGTT CTCTCAGTTC TCTTAGTATT
    GATTCTGAAG ATGACCTGCT GCAGGAATGT ATAAGTTCTG CAATGCCAAA AAAGAAAAAG
    CCTTCAAGGC TCAAGCCCGA TAATGAAAAG CATAGTCCCA GAAATATGGG TGGCATATTA
    GCAGAAGATT TGACACTCGA TTTGAAAGAT ATACAGAGAC CAGATTCAGA ACATGGTTTA
    TCCCCAGATT CAGAAAATTT TGATTGGAAA GCTATTCAGG AAGGTGCAAA TTCCATAGTA
    AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA TGTTTATCTC GACAAGCTTC ATCTGATTCA
    GATTCTATCC TTTCATTGAA ATCGGGTATC TCTCTGGGAT CACCATTTCA CCTTACACCT
    GATCAAGAAG AAAAACCCTT TACGAGTAAT AAAGGTCCAC GAATTCTAAA ACCTGGGGAG
    AAAAGTACAT TGGAAACTAA AAAAATAGAA TCTGAAAATA AAGGAATAAA AGGAGGCAAA
    AAGGTTTATA AAAGTTTGAT TACTGGAAAA GTTCGATCTA ATTCGGAAAT TTCGAGCCAA
    ATGAAACAAC CCCTTCAAAC AAACATGCCT TCAATCTCTC GAGGTAGGAC AATGATTCAT
    ATTCCAGGAG TTCGGAATAG CTCTTCAAGT ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC
    CTCAAGACTC CAGCCTCCAA AAGCCCTAGT GAAGGTCAGC CGGCTACCAC CTCTCCCAGA
    GGAACCAAGC CATCAGTGAA GTCAGAATTA AGCCCTGTTA CAAGGCAGGC ATCCCAGACA
    GCAGGATCAA ACAAAGGACC TTCTAGATCA GGATCTAGAG ATTCCACTCC TTCAAGACCT
    GCCCAGCAAC CATTAAGTAG ACCAATGCAG TCTCCAGGGC GAAACTCAAT CTCTCCTGGT
    AGAAATGGAA TAAGTCCCCC TAACAAATTA TCTCAACTAC CAAGGACGTC ATCCCCTAGT
    ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG AAAATGTCTT ACACATCTCC TGGCAGACAG
    ATGAGCCAGC AGAACCTCAC CAAACAAACA GGCTTATCCA AGAATGGCAG TGGTATCCCA
    AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT CAAATGAGTA ATAGTAATGG ATCCAATAAA
    AAAGTAGAAC TTTCTAGAAT GTCTTCAACA AAGTCAAGTG GAAGTGAATC CGATAGGTCA
    GAGAGACCTG TATTAGTACG CCAATCAACT TTCATCAAAG AAGCTCCAAG CCCAACCCTA
    AGGAGAAAAC TGGAGGAATC TGCTTCATTT GAATCTCTTT CTCCATCTTC CAGACCCGAT
    TCTCCCACAC GGTCCCAGGC TCATACTCCA GTTTTAAGTC CTTCCCTTCC TGATATGTCT
    CTATCTACAC ATTCGTCTCT TCAGTCTGGT GGATGGCGAA AACTCCCACC TAACCTCAGT
    CCCACCATAG AGTATAATGA TGGAAGACCA GTAAAGCGCC ATGATATAGC ACGCTCTCAT
    TCCGAAAGTC CTTCCAGACT TCCCATCAAT AGGTCAGGGA CCTGGAAACG TGAGCACAGC
    AAACACTCAT CATCACTTCC TCGAGTAAGC ACTTGGAGAA GAACTGGAAG TTCATCCTCA
    ATTCTTTCTG CTTCATCAGA ATCTAGTGAA AAGGCAAAAA GTGAGGACGA AAAACAAGTG
    AACTCTATTT CAGGAAGCAA ACAAACTAAA GAAAACCAGG TATCCACAAA AGGAACATGG
    AGAAAAATAA AAGAAAGTGA AATTTCTCCC ACAAATAGTA CTTCTCAGAC CACTTCTTCA
    GGTGCTGCAA ATGGTGCTGA ATCAAAGACT CTGATTTATC AAATGGCACC TGCTGTTTCT
    AAAACAGAGG ATGTTTGGGT GAGAATTGAG GATTGCCCCA TTAACAACCC TAGATCTGGA
    AGATCTCCAA CAGGAAATAC TCCCCCCGTG ATTGACACTG TTTCAGAAAA GGGAAACCCG
    AACCCTAAAG ATTCAAAAGA TAATCAGGGA AAACAAAATG TGAGCAATGG TAGTGCCCCT
    ACACGCACCA TGGGTCTGGA AAACCGCCTG AATTCCTTTA TTCAGGTAGA TCCTCCAGAC
    CAAAAAGGAA CTGAGACAAA ACCGGGACAC AGTAATAACC CTGTCCCTGC ATCCGAGACT
    AGTGAAAGTT CTATAGCCGA GCGTACCCCG TTTAGTTCTA GCAGCTCAAG CAAGCACAGT
    TCACCAAGTG GGACTGTCGC CGCCAGGGTG AGTCCTTTTA ATTACAACCC AAGCCCAAGG
    AAGAGCAGCA CAGATGGCAC TTCAGCCCGA CCGTCTCAGA TCCCAACGCC AGTGAGCAAC
    AACACAAAGA AACGCGACTC AAAACCCGAC AGCACAGAAC CCAGCGGGAC TCAAAGTCCT
    AAACGCCATT CTGGGTCTTA CCTTGTGACA TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219611
    Clone ID Related Accession (Same CDS sequence) XM_015472980.2 , XM_015472982.2 , XM_015472981.2
    Accession Version XM_015472980.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    481
    541
    601
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1801
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    1981
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    7921
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    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CACCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328466.1
    CDS329..9037
    Translation

    Target ORF information:

    RefSeq Version XM_015472980.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472980.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CACCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219611
    Clone ID Related Accession (Same CDS sequence) XM_015472980.2 , XM_015472982.2 , XM_015472981.2
    Accession Version XM_015472982.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CACCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328468.1
    CDS43..8751
    Translation

    Target ORF information:

    RefSeq Version XM_015472982.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472982.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CACCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219611
    Clone ID Related Accession (Same CDS sequence) XM_015472980.2 , XM_015472982.2 , XM_015472981.2
    Accession Version XM_015472981.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CACCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328467.1
    CDS95..8803
    Translation

    Target ORF information:

    RefSeq Version XM_015472981.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472981.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CACCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219612
    Clone ID Related Accession (Same CDS sequence) XM_015472983.2
    Accession Version XM_015472983.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    541
    601
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    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
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    4141
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    5041
    5101
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
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    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
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    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGGG GACTACAGGC CATTGCAGAA
    TTATTGCAAG TGGACTGTGA AATGTATGGG CTTACTAATG ACCACTACAG TATTACTTTA
    AGACGATATG CAGGAATGGC TTTGACAAAC TTGACTTTCG GAGATGTAGC CAACAAGGCT
    ACTCTCTGCT CTATGAAAGG CTGCATGAGA GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC
    GAGGACTTGC AGCAGGTTAT TGCGAGTGTT TTGAGGAATC TGTCTTGGAG AGCAGATGTA
    AATAGTAAAA AGACTTTGCG TGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG
    GAAGTGAAAA AGGAATCAAC CCTCAAAAGT GTATTGAGTG CCTTATGGAA CTTGTCAGCA
    CACTGCACTG AGAATAAAGC TGACATATGT GCTGTGGATG GTGCGCTTGC ATTTTTGGTT
    GGCACTCTCA CTTATCGCAG CCAGACCAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGT
    ATATTACGGA ATGTATCCAG CTTGATAGCT ACAAATGAGG ACCACAGGCA AATCCTAAGA
    GAGAATAATT GCTTACAGAC CTTATTACAA CACTTGAAAT CTCACAGTTT GACAATAGTC
    AGCAATGCAT GCGGAACCTT GTGGAATCTC TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA
    TTGTGGGACA TGGGAGCAGT CAGCATGCTC AAGAACCTCA TTCATTCAAA GCACAAGATG
    ATTGCTATGG GAAGTGCTGC AGCTTTAAGG AATCTCATGG CAAATAGACC TGCAAAGTAT
    AAAGATGCCA ATATCATGTC TCCTGGTTCA AGTTTGCCTT CTCTACATGT CAGGAAACAA
    AAGGCCCTGG AAGCAGAATT AGATGCTCAG CATTTATCAG AAACTTTTGA CAATATTGAC
    AATTTAAGTC CCAAGGCATC TCATCGTAGC AAGCAGAGAC ACAAGCAAAA TCTCTATGGT
    GACTATGTTT TTGACACCAA TCGACATGAT GATAACAGGT CAGACAATTT TAATACTGGA
    AACATGACTG TCCTATCACC GTACTTAAAC ACTACAGTAT TGCCCAGCTC TTCTTCATCA
    AGGGGAAGTT TAGATAGCTC TCGTTCTGAG AAAGATAGAA GTCTGGAGAG AGAACGAGGT
    ATTAGCCTAG GCAACTATCA CCCAGCAACA GAAAACCCAG GAACCTCTTC AAAGCGAGGT
    TTGCAGATTT CTACCACTGC AGCCCAGATT GCCAAAGTCA TGGAAGAAGT ATCAGCTATT
    CATACCTCTC AGGAAGACAG AAGTTCTGGG TCTACCACAG AACTACACTG TGGGACAGAT
    GAGAGGAATG CACTAAGAAG AAGCTCTACC ACCCACACAC ATGCAAACAC GTACAACTTT
    ACCAAGTCAG AAAACTCAAA CAGAACATGT CCAATACCAT ATGCCAAAGT AGAATATAAG
    AGATCTTCAA ATGATAGTTT AAATAGTGTC AGCAGTAGTG ATGGTTATGG TAAAAGAGGT
    CAGATGAAAC CTTCAATTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGCTAT
    GGTCAGTATC CAGCTGACCT AGCCCATAAA ATACATAGTG CAAATCATAT GGATGATAAT
    GATGGAGAAC TAGATACACC AATAAATTAC AGTCTCAAAT ATTCCGATGA ACAGTTGAAC
    TCTGGGAGGC AGAGCCCTTC ACAGAATGAA AGATGGGCAA GACCCAAACA TATACTAGAA
    GATGAAATAA AACCGAATGA GCAGAGACAA TCAAGGAGTC AAAGCACAGC TTATCCCGTG
    TATCCTGAGA GCACTGATGA TAAACACCTC AAGTTCCAAC CACACTTTGG GCAGCAAGAA
    TGTGTTTCCC CATACAGGTC AAGAGCAGCC AATGGATCTG AAACAAATCG AGTAGGCTCT
    AATCATGGAA TTAGTCAAAA TGTGAACCAG TCTTTGTGTC AAGAAGATGA CTATGAAGAC
    GATAAACCAA CCAACTATAG CGAACGTTAC TCTGAGGAAG GGCAGCATGA GGAAGAAGAG
    AGGCCAACCA ATTATAGCAT AAAATACAGT GAAGAAAAAC ATCACGTGGA TCAGCCTATT
    GATTATAGTT TAAAATACAC CACAGACATT CCTTCTTCAC AGAAACCAGC ATTTTCATTC
    TCAAAGAATT CATCTGGACA GAGCACAAAA ACTGAACACA TCTCTTCAAG CAGCGAGAAT
    ACATCCACAA CTTCATCTAA TGCCAAGAGG CAGAATCAGC TGCATCCAAG CTCAGCACAG
    AGCAGAAGTG GTCAGACCCC AAAAGCCACC TCTTCCTCTT GCAAAGTCCC CTCTATCAAC
    CAAGAAACAA TACAGACTTA CTGTGTAGAA GATACCCCAA TATGTTTTTC AAGATGCAGT
    TCATTATCAT CTCTGTCATC TGCTGAAGAT GAAGTAGGGT GTGATCAGAC AACACAAGAA
    GCAGAGTCTG CTAACACTCT GCAAATAGCT GAAATCAAGG ACAACAGCGG ACCTAGGTCA
    AATGAAGATT CTGTGAGTAA AGTTCCAGCA GGGTCACAGC ACATTAGAAC CAAATCCAGC
    AGACTCCAGG CTTCTGGTCT GTCTTCAGAG TCAGCCAGGC ACAAAGCTGT TGAATTTTCT
    TCAGGGGCCA AATCTCCTTC CAAGAGCGGT GCTCAGACAC CCAAAAGTCC ACCAGAGCAC
    TACGTTCAGG AGACCCCACT CATGTTTAGC AGATGTACTT CAGTCAGTTC ACTCGACAGT
    TTTGAGAGTC GCTCGATTGC CAGCTCTGTT CAGAGTGAAC CCTGCAGTGG AATGGTAAGT
    GGCATCATAA GCCCCAGTGA CCTCCCAGAT AGCCCTGGAC AAACCATGCC ACCAAGCAGA
    AGCAAAACCC CTCCGCCACC GCCTCCGCCT CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA
    GTACCTAAAA ATAAAGCACC TAGTGCTGAA AAGAGAGAAA GTGGACCCAA GCAAGCTGCT
    GTAAATGCTG CAGTACAAAG GGTCCAGGTT CTTCCAGAGG CTGATACTCT GTTACATTTT
    GCCACAGAGA GTACTCCTGA TGGATTTTCT TGTTCATCTA GCCTGAGCGC TCTGAGCCTT
    GATGAGCCAT TTATTCAGAA AGATGTGGAA CTAAGAATAA TGCCTCCAGT TCAGGAAAAT
    GACAACGGGA ATGAAACAGA AAGTGAGCAG CCTGAAGAAT CAAATGAAAA CCAGGAAAAA
    GAGGCAGAAA AACCCACTGA TTCTGAAAAA GATCTTTTAG ATGAGTCAGA TGATGATGAT
    ATTGAAATAC TAGAAGAGTG TATAATTTCT GCCATGCCAA CAAAATCTTC ACGCAAAGCC
    AAAAAACCAG CCCAGACTAC TTCAAAATTA CCTCCACCTG TGGCAAGGAA ACCAAGTCAG
    CTCCCTGTAT ACAAACTTCT GCCATCGCAA AACAGGTTAC AGGCACAAAA GCATGTTAGT
    TTTACACCAG GAGATGATAT GCCACGGGTG TATTGTGTAG AAGGGACACC TATAAACTTT
    TCCACAGCTA CATCTCTGAG TGATCTAACG ATAGAATCCC CTCCAAATGA GTTAGCTGCT
    GGAGAAGGGG TTAGAGCAGG GGCACAGTCA AGTGAGTTTG AAAAACGAGA TACCATTCCT
    ACTGAAGGCA GAAGTACAGA TGAGGCTCAA CGAGGGAAAG CCTCATCTGT CACTGTGCCT
    GAACTGGACG ACAGCAAAAC AGAAGAAGGT GATATTCTTG CAGAATGCAT TAATTCTGCC
    ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC CGTGTGAAAA AGATCATGGA CCAGGTCCAG
    CAAGCATCTA TGTCTTCATC TGGAACTAAC AAAAATCAAT TAGATGGTAA GACGAAGAAA
    CCTACTTCAC CAGTAAAACC TATACCACAA AATACTGAAT ACAGGACACG TGTAAGAAAA
    AATACAGACT CAAAAAATAA TCTAAATGCA GAAAGAAATT TCTCGGAAAA CAAAGACTCA
    AAGAAACAGC ACTTGAAAAA TAATTCCAAG GACTTCAATG ATAAACTGCC AAATAATGAA
    GACAGAGTCA GAGGAAGTTT TACTTTTGAT TCACCCCATC ATTACACGCC CATTGAAGGC
    ACTCCGTACT GTTTTTCACG AAATGACTCT CTGAGTTCTC TAGATTTTGA TGATGATGAT
    GTTGACCTTT CCAGGGAAAA GGCTGAATTA AGAAAGGGGA AGGAAAATAA AGAATCAGAA
    GCTAAAGTGA CCAACCACAC AGAACTAACC TCAAACCAAC AATCAGCTAG TAAGACACCA
    GCTGTTACAA AGCAGCCAAT AAATAGAGGT CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC
    ACTTTTCCCC AGTCTTCCAA AGATATACCA GACAGAGGGG CAGCAACAGA TGAGAAATTA
    CAGAATTTCG CTATTGAAAA TACTCCAGTT TGCTTTTCTC GAAATTCCTC TCTAAGCTCT
    CTTAGTGACA TTGATCAAGA AAACAACAAC AACAAGGAAA ATGAACCTGT CAAAGAGACA
    GAGCCCCCTG CCTCACAGGG AGAACCAGGT AAACCCCAGG CCTCAGGTTA TGCTCCTAAA
    TCGTTTCACG TGGAAGACAC CCCTGTTTGT TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT
    AGTATTGATT CTGAAGATGA CCTGCTGCAG GAATGTATAA GTTCTGCAAT GCCAAAAAAG
    AAAAAGCCTT CAAGGCTCAA GCCCGATAAT GAAAAGCATA GTCCCAGAAA TATGGGTGGC
    ATATTAGCAG AAGATTTGAC ACTCGATTTG AAAGATATAC AGAGACCAGA TTCAGAACAT
    GGTTTATCCC CAGATTCAGA AAATTTTGAT TGGAAAGCTA TTCAGGAAGG TGCAAATTCC
    ATAGTAAGTA GTTTACATCA AGCTGCTGCC GCTGCATGTT TATCTCGACA AGCTTCATCT
    GATTCAGATT CTATCCTTTC ATTGAAATCG GGTATCTCTC TGGGATCACC ATTTCACCTT
    ACACCTGATC AAGAAGAAAA ACCCTTTACG AGTAATAAAG GTCCACGAAT TCTAAAACCT
    GGGGAGAAAA GTACATTGGA AACTAAAAAA ATAGAATCTG AAAATAAAGG AATAAAAGGA
    GGCAAAAAGG TTTATAAAAG TTTGATTACT GGAAAAGTTC GATCTAATTC GGAAATTTCG
    AGCCAAATGA AACAACCCCT TCAAACAAAC ATGCCTTCAA TCTCTCGAGG TAGGACAATG
    ATTCATATTC CAGGAGTTCG GAATAGCTCT TCAAGTACAA GCCCAGTTTC TAAAAAAGGC
    CCGCCCCTCA AGACTCCAGC CTCCAAAAGC CCTAGTGAAG GTCAGCCGGC TACCACCTCT
    CCCAGAGGAA CCAAGCCATC AGTGAAGTCA GAATTAAGCC CTGTTACAAG GCAGGCATCC
    CAGACAGCAG GATCAAACAA AGGACCTTCT AGATCAGGAT CTAGAGATTC CACTCCTTCA
    AGACCTGCCC AGCAACCATT AAGTAGACCA ATGCAGTCTC CAGGGCGAAA CTCAATCTCT
    CCTGGTAGAA ATGGAATAAG TCCCCCTAAC AAATTATCTC AACTACCAAG GACGTCATCC
    CCTAGTACTG CTTCAACTAA GTCCTCAGGT TCTGGGAAAA TGTCTTACAC ATCTCCTGGC
    AGACAGATGA GCCAGCAGAA CCTCACCAAA CAAACAGGCT TATCCAAGAA TGGCAGTGGT
    ATCCCAAGAA GTGAATCTGC CTCCAAAGGG CTAAATCAAA TGAGTAATAG TAATGGATCC
    AATAAAAAAG TAGAACTTTC TAGAATGTCT TCAACAAAGT CAAGTGGAAG TGAATCCGAT
    AGGTCAGAGA GACCTGTATT AGTACGCCAA TCAACTTTCA TCAAAGAAGC TCCAAGCCCA
    ACCCTAAGGA GAAAACTGGA GGAATCTGCT TCATTTGAAT CTCTTTCTCC ATCTTCCAGA
    CCCGATTCTC CCACACGGTC CCAGGCTCAT ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT
    ATGTCTCTAT CTACACATTC GTCTCTTCAG TCTGGTGGAT GGCGAAAACT CCCACCTAAC
    CTCAGTCCCA CCATAGAGTA TAATGATGGA AGACCAGTAA AGCGCCATGA TATAGCACGC
    TCTCATTCCG AAAGTCCTTC CAGACTTCCC ATCAATAGGT CAGGGACCTG GAAACGTGAG
    CACAGCAAAC ACTCATCATC ACTTCCTCGA GTAAGCACTT GGAGAAGAAC TGGAAGTTCA
    TCCTCAATTC TTTCTGCTTC ATCAGAATCT AGTGAAAAGG CAAAAAGTGA GGACGAAAAA
    CAAGTGAACT CTATTTCAGG AAGCAAACAA ACTAAAGAAA ACCAGGTATC CACAAAAGGA
    ACATGGAGAA AAATAAAAGA AAGTGAAATT TCTCCCACAA ATAGTACTTC TCAGACCACT
    TCTTCAGGTG CTGCAAATGG TGCTGAATCA AAGACTCTGA TTTATCAAAT GGCACCTGCT
    GTTTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGATT GCCCCATTAA CAACCCTAGA
    TCTGGAAGAT CTCCAACAGG AAATACTCCC CCCGTGATTG ACACTGTTTC AGAAAAGGGA
    AACCCGAACC CTAAAGATTC AAAAGATAAT CAGGGAAAAC AAAATGTGAG CAATGGTAGT
    GCCCCTACAC GCACCATGGG TCTGGAAAAC CGCCTGAATT CCTTTATTCA GGTAGATCCT
    CCAGACCAAA AAGGAACTGA GACAAAACCG GGACACAGTA ATAACCCTGT CCCTGCATCC
    GAGACTAGTG AAAGTTCTAT AGCCGAGCGT ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG
    CACAGTTCAC CAAGTGGGAC TGTCGCCGCC AGGGTGAGTC CTTTTAATTA CAACCCAAGC
    CCAAGGAAGA GCAGCACAGA TGGCACTTCA GCCCGACCGT CTCAGATCCC AACGCCAGTG
    AGCAACAACA CAAAGAAACG CGACTCAAAA CCCGACAGCA CAGAACCCAG CGGGACTCAA
    AGTCCTAAAC GCCATTCTGG GTCTTACCTT GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328469.1
    CDS132..8816
    Translation

    Target ORF information:

    RefSeq Version XM_015472983.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472983.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGGG GACTACAGGC CATTGCAGAA
    TTATTGCAAG TGGACTGTGA AATGTATGGG CTTACTAATG ACCACTACAG TATTACTTTA
    AGACGATATG CAGGAATGGC TTTGACAAAC TTGACTTTCG GAGATGTAGC CAACAAGGCT
    ACTCTCTGCT CTATGAAAGG CTGCATGAGA GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC
    GAGGACTTGC AGCAGGTTAT TGCGAGTGTT TTGAGGAATC TGTCTTGGAG AGCAGATGTA
    AATAGTAAAA AGACTTTGCG TGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG
    GAAGTGAAAA AGGAATCAAC CCTCAAAAGT GTATTGAGTG CCTTATGGAA CTTGTCAGCA
    CACTGCACTG AGAATAAAGC TGACATATGT GCTGTGGATG GTGCGCTTGC ATTTTTGGTT
    GGCACTCTCA CTTATCGCAG CCAGACCAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGT
    ATATTACGGA ATGTATCCAG CTTGATAGCT ACAAATGAGG ACCACAGGCA AATCCTAAGA
    GAGAATAATT GCTTACAGAC CTTATTACAA CACTTGAAAT CTCACAGTTT GACAATAGTC
    AGCAATGCAT GCGGAACCTT GTGGAATCTC TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA
    TTGTGGGACA TGGGAGCAGT CAGCATGCTC AAGAACCTCA TTCATTCAAA GCACAAGATG
    ATTGCTATGG GAAGTGCTGC AGCTTTAAGG AATCTCATGG CAAATAGACC TGCAAAGTAT
    AAAGATGCCA ATATCATGTC TCCTGGTTCA AGTTTGCCTT CTCTACATGT CAGGAAACAA
    AAGGCCCTGG AAGCAGAATT AGATGCTCAG CATTTATCAG AAACTTTTGA CAATATTGAC
    AATTTAAGTC CCAAGGCATC TCATCGTAGC AAGCAGAGAC ACAAGCAAAA TCTCTATGGT
    GACTATGTTT TTGACACCAA TCGACATGAT GATAACAGGT CAGACAATTT TAATACTGGA
    AACATGACTG TCCTATCACC GTACTTAAAC ACTACAGTAT TGCCCAGCTC TTCTTCATCA
    AGGGGAAGTT TAGATAGCTC TCGTTCTGAG AAAGATAGAA GTCTGGAGAG AGAACGAGGT
    ATTAGCCTAG GCAACTATCA CCCAGCAACA GAAAACCCAG GAACCTCTTC AAAGCGAGGT
    TTGCAGATTT CTACCACTGC AGCCCAGATT GCCAAAGTCA TGGAAGAAGT ATCAGCTATT
    CATACCTCTC AGGAAGACAG AAGTTCTGGG TCTACCACAG AACTACACTG TGGGACAGAT
    GAGAGGAATG CACTAAGAAG AAGCTCTACC ACCCACACAC ATGCAAACAC GTACAACTTT
    ACCAAGTCAG AAAACTCAAA CAGAACATGT CCAATACCAT ATGCCAAAGT AGAATATAAG
    AGATCTTCAA ATGATAGTTT AAATAGTGTC AGCAGTAGTG ATGGTTATGG TAAAAGAGGT
    CAGATGAAAC CTTCAATTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGCTAT
    GGTCAGTATC CAGCTGACCT AGCCCATAAA ATACATAGTG CAAATCATAT GGATGATAAT
    GATGGAGAAC TAGATACACC AATAAATTAC AGTCTCAAAT ATTCCGATGA ACAGTTGAAC
    TCTGGGAGGC AGAGCCCTTC ACAGAATGAA AGATGGGCAA GACCCAAACA TATACTAGAA
    GATGAAATAA AACCGAATGA GCAGAGACAA TCAAGGAGTC AAAGCACAGC TTATCCCGTG
    TATCCTGAGA GCACTGATGA TAAACACCTC AAGTTCCAAC CACACTTTGG GCAGCAAGAA
    TGTGTTTCCC CATACAGGTC AAGAGCAGCC AATGGATCTG AAACAAATCG AGTAGGCTCT
    AATCATGGAA TTAGTCAAAA TGTGAACCAG TCTTTGTGTC AAGAAGATGA CTATGAAGAC
    GATAAACCAA CCAACTATAG CGAACGTTAC TCTGAGGAAG GGCAGCATGA GGAAGAAGAG
    AGGCCAACCA ATTATAGCAT AAAATACAGT GAAGAAAAAC ATCACGTGGA TCAGCCTATT
    GATTATAGTT TAAAATACAC CACAGACATT CCTTCTTCAC AGAAACCAGC ATTTTCATTC
    TCAAAGAATT CATCTGGACA GAGCACAAAA ACTGAACACA TCTCTTCAAG CAGCGAGAAT
    ACATCCACAA CTTCATCTAA TGCCAAGAGG CAGAATCAGC TGCATCCAAG CTCAGCACAG
    AGCAGAAGTG GTCAGACCCC AAAAGCCACC TCTTCCTCTT GCAAAGTCCC CTCTATCAAC
    CAAGAAACAA TACAGACTTA CTGTGTAGAA GATACCCCAA TATGTTTTTC AAGATGCAGT
    TCATTATCAT CTCTGTCATC TGCTGAAGAT GAAGTAGGGT GTGATCAGAC AACACAAGAA
    GCAGAGTCTG CTAACACTCT GCAAATAGCT GAAATCAAGG ACAACAGCGG ACCTAGGTCA
    AATGAAGATT CTGTGAGTAA AGTTCCAGCA GGGTCACAGC ACATTAGAAC CAAATCCAGC
    AGACTCCAGG CTTCTGGTCT GTCTTCAGAG TCAGCCAGGC ACAAAGCTGT TGAATTTTCT
    TCAGGGGCCA AATCTCCTTC CAAGAGCGGT GCTCAGACAC CCAAAAGTCC ACCAGAGCAC
    TACGTTCAGG AGACCCCACT CATGTTTAGC AGATGTACTT CAGTCAGTTC ACTCGACAGT
    TTTGAGAGTC GCTCGATTGC CAGCTCTGTT CAGAGTGAAC CCTGCAGTGG AATGGTAAGT
    GGCATCATAA GCCCCAGTGA CCTCCCAGAT AGCCCTGGAC AAACCATGCC ACCAAGCAGA
    AGCAAAACCC CTCCGCCACC GCCTCCGCCT CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA
    GTACCTAAAA ATAAAGCACC TAGTGCTGAA AAGAGAGAAA GTGGACCCAA GCAAGCTGCT
    GTAAATGCTG CAGTACAAAG GGTCCAGGTT CTTCCAGAGG CTGATACTCT GTTACATTTT
    GCCACAGAGA GTACTCCTGA TGGATTTTCT TGTTCATCTA GCCTGAGCGC TCTGAGCCTT
    GATGAGCCAT TTATTCAGAA AGATGTGGAA CTAAGAATAA TGCCTCCAGT TCAGGAAAAT
    GACAACGGGA ATGAAACAGA AAGTGAGCAG CCTGAAGAAT CAAATGAAAA CCAGGAAAAA
    GAGGCAGAAA AACCCACTGA TTCTGAAAAA GATCTTTTAG ATGAGTCAGA TGATGATGAT
    ATTGAAATAC TAGAAGAGTG TATAATTTCT GCCATGCCAA CAAAATCTTC ACGCAAAGCC
    AAAAAACCAG CCCAGACTAC TTCAAAATTA CCTCCACCTG TGGCAAGGAA ACCAAGTCAG
    CTCCCTGTAT ACAAACTTCT GCCATCGCAA AACAGGTTAC AGGCACAAAA GCATGTTAGT
    TTTACACCAG GAGATGATAT GCCACGGGTG TATTGTGTAG AAGGGACACC TATAAACTTT
    TCCACAGCTA CATCTCTGAG TGATCTAACG ATAGAATCCC CTCCAAATGA GTTAGCTGCT
    GGAGAAGGGG TTAGAGCAGG GGCACAGTCA AGTGAGTTTG AAAAACGAGA TACCATTCCT
    ACTGAAGGCA GAAGTACAGA TGAGGCTCAA CGAGGGAAAG CCTCATCTGT CACTGTGCCT
    GAACTGGACG ACAGCAAAAC AGAAGAAGGT GATATTCTTG CAGAATGCAT TAATTCTGCC
    ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC CGTGTGAAAA AGATCATGGA CCAGGTCCAG
    CAAGCATCTA TGTCTTCATC TGGAACTAAC AAAAATCAAT TAGATGGTAA GACGAAGAAA
    CCTACTTCAC CAGTAAAACC TATACCACAA AATACTGAAT ACAGGACACG TGTAAGAAAA
    AATACAGACT CAAAAAATAA TCTAAATGCA GAAAGAAATT TCTCGGAAAA CAAAGACTCA
    AAGAAACAGC ACTTGAAAAA TAATTCCAAG GACTTCAATG ATAAACTGCC AAATAATGAA
    GACAGAGTCA GAGGAAGTTT TACTTTTGAT TCACCCCATC ATTACACGCC CATTGAAGGC
    ACTCCGTACT GTTTTTCACG AAATGACTCT CTGAGTTCTC TAGATTTTGA TGATGATGAT
    GTTGACCTTT CCAGGGAAAA GGCTGAATTA AGAAAGGGGA AGGAAAATAA AGAATCAGAA
    GCTAAAGTGA CCAACCACAC AGAACTAACC TCAAACCAAC AATCAGCTAG TAAGACACCA
    GCTGTTACAA AGCAGCCAAT AAATAGAGGT CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC
    ACTTTTCCCC AGTCTTCCAA AGATATACCA GACAGAGGGG CAGCAACAGA TGAGAAATTA
    CAGAATTTCG CTATTGAAAA TACTCCAGTT TGCTTTTCTC GAAATTCCTC TCTAAGCTCT
    CTTAGTGACA TTGATCAAGA AAACAACAAC AACAAGGAAA ATGAACCTGT CAAAGAGACA
    GAGCCCCCTG CCTCACAGGG AGAACCAGGT AAACCCCAGG CCTCAGGTTA TGCTCCTAAA
    TCGTTTCACG TGGAAGACAC CCCTGTTTGT TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT
    AGTATTGATT CTGAAGATGA CCTGCTGCAG GAATGTATAA GTTCTGCAAT GCCAAAAAAG
    AAAAAGCCTT CAAGGCTCAA GCCCGATAAT GAAAAGCATA GTCCCAGAAA TATGGGTGGC
    ATATTAGCAG AAGATTTGAC ACTCGATTTG AAAGATATAC AGAGACCAGA TTCAGAACAT
    GGTTTATCCC CAGATTCAGA AAATTTTGAT TGGAAAGCTA TTCAGGAAGG TGCAAATTCC
    ATAGTAAGTA GTTTACATCA AGCTGCTGCC GCTGCATGTT TATCTCGACA AGCTTCATCT
    GATTCAGATT CTATCCTTTC ATTGAAATCG GGTATCTCTC TGGGATCACC ATTTCACCTT
    ACACCTGATC AAGAAGAAAA ACCCTTTACG AGTAATAAAG GTCCACGAAT TCTAAAACCT
    GGGGAGAAAA GTACATTGGA AACTAAAAAA ATAGAATCTG AAAATAAAGG AATAAAAGGA
    GGCAAAAAGG TTTATAAAAG TTTGATTACT GGAAAAGTTC GATCTAATTC GGAAATTTCG
    AGCCAAATGA AACAACCCCT TCAAACAAAC ATGCCTTCAA TCTCTCGAGG TAGGACAATG
    ATTCATATTC CAGGAGTTCG GAATAGCTCT TCAAGTACAA GCCCAGTTTC TAAAAAAGGC
    CCGCCCCTCA AGACTCCAGC CTCCAAAAGC CCTAGTGAAG GTCAGCCGGC TACCACCTCT
    CCCAGAGGAA CCAAGCCATC AGTGAAGTCA GAATTAAGCC CTGTTACAAG GCAGGCATCC
    CAGACAGCAG GATCAAACAA AGGACCTTCT AGATCAGGAT CTAGAGATTC CACTCCTTCA
    AGACCTGCCC AGCAACCATT AAGTAGACCA ATGCAGTCTC CAGGGCGAAA CTCAATCTCT
    CCTGGTAGAA ATGGAATAAG TCCCCCTAAC AAATTATCTC AACTACCAAG GACGTCATCC
    CCTAGTACTG CTTCAACTAA GTCCTCAGGT TCTGGGAAAA TGTCTTACAC ATCTCCTGGC
    AGACAGATGA GCCAGCAGAA CCTCACCAAA CAAACAGGCT TATCCAAGAA TGGCAGTGGT
    ATCCCAAGAA GTGAATCTGC CTCCAAAGGG CTAAATCAAA TGAGTAATAG TAATGGATCC
    AATAAAAAAG TAGAACTTTC TAGAATGTCT TCAACAAAGT CAAGTGGAAG TGAATCCGAT
    AGGTCAGAGA GACCTGTATT AGTACGCCAA TCAACTTTCA TCAAAGAAGC TCCAAGCCCA
    ACCCTAAGGA GAAAACTGGA GGAATCTGCT TCATTTGAAT CTCTTTCTCC ATCTTCCAGA
    CCCGATTCTC CCACACGGTC CCAGGCTCAT ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT
    ATGTCTCTAT CTACACATTC GTCTCTTCAG TCTGGTGGAT GGCGAAAACT CCCACCTAAC
    CTCAGTCCCA CCATAGAGTA TAATGATGGA AGACCAGTAA AGCGCCATGA TATAGCACGC
    TCTCATTCCG AAAGTCCTTC CAGACTTCCC ATCAATAGGT CAGGGACCTG GAAACGTGAG
    CACAGCAAAC ACTCATCATC ACTTCCTCGA GTAAGCACTT GGAGAAGAAC TGGAAGTTCA
    TCCTCAATTC TTTCTGCTTC ATCAGAATCT AGTGAAAAGG CAAAAAGTGA GGACGAAAAA
    CAAGTGAACT CTATTTCAGG AAGCAAACAA ACTAAAGAAA ACCAGGTATC CACAAAAGGA
    ACATGGAGAA AAATAAAAGA AAGTGAAATT TCTCCCACAA ATAGTACTTC TCAGACCACT
    TCTTCAGGTG CTGCAAATGG TGCTGAATCA AAGACTCTGA TTTATCAAAT GGCACCTGCT
    GTTTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGATT GCCCCATTAA CAACCCTAGA
    TCTGGAAGAT CTCCAACAGG AAATACTCCC CCCGTGATTG ACACTGTTTC AGAAAAGGGA
    AACCCGAACC CTAAAGATTC AAAAGATAAT CAGGGAAAAC AAAATGTGAG CAATGGTAGT
    GCCCCTACAC GCACCATGGG TCTGGAAAAC CGCCTGAATT CCTTTATTCA GGTAGATCCT
    CCAGACCAAA AAGGAACTGA GACAAAACCG GGACACAGTA ATAACCCTGT CCCTGCATCC
    GAGACTAGTG AAAGTTCTAT AGCCGAGCGT ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG
    CACAGTTCAC CAAGTGGGAC TGTCGCCGCC AGGGTGAGTC CTTTTAATTA CAACCCAAGC
    CCAAGGAAGA GCAGCACAGA TGGCACTTCA GCCCGACCGT CTCAGATCCC AACGCCAGTG
    AGCAACAACA CAAAGAAACG CGACTCAAAA CCCGACAGCA CAGAACCCAG CGGGACTCAA
    AGTCCTAAAC GCCATTCTGG GTCTTACCTT GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219613
    Clone ID Related Accession (Same CDS sequence) XM_010808816.3
    Accession Version XM_010808816.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCACCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807118.1
    CDS132..8771
    Translation

    Target ORF information:

    RefSeq Version XM_010808816.3
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808816.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCACCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219614
    Clone ID Related Accession (Same CDS sequence) XM_024997640.1
    Accession Version XM_024997640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1501
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    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGT AGGAAGGCTA CCCGGGGCAT TTCATCCCAG
    GAGCTAGGGC AGGGGCTTTC AGGGGGACTA CAGGCCATTG CAGAATTATT GCAAGTGGAC
    TGTGAAATGT ATGGGCTTAC TAATGACCAC TACAGTATTA CTTTAAGACG ATATGCAGGA
    ATGGCTTTGA CAAACTTGAC TTTCGGAGAT GTAGCCAACA AGGCTACTCT CTGCTCTATG
    AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG
    GTTATTGCGA GTGTTTTGAG GAATCTGTCT TGGAGAGCAG ATGTAAATAG TAAAAAGACT
    TTGCGTGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT GAAAAAGGAA
    TCAACCCTCA AAAGTGTATT GAGTGCCTTA TGGAACTTGT CAGCACACTG CACTGAGAAT
    AAAGCTGACA TATGTGCTGT GGATGGTGCG CTTGCATTTT TGGTTGGCAC TCTCACTTAT
    CGCAGCCAGA CCAATACTTT AGCCATTATT GAAAGTGGAG GTGGTATATT ACGGAATGTA
    TCCAGCTTGA TAGCTACAAA TGAGGACCAC AGGCAAATCC TAAGAGAGAA TAATTGCTTA
    CAGACCTTAT TACAACACTT GAAATCTCAC AGTTTGACAA TAGTCAGCAA TGCATGCGGA
    ACCTTGTGGA ATCTCTCAGC AAGAAACCCT AAAGACCAGG AAGCATTGTG GGACATGGGA
    GCAGTCAGCA TGCTCAAGAA CCTCATTCAT TCAAAGCACA AGATGATTGC TATGGGAAGT
    GCTGCAGCTT TAAGGAATCT CATGGCAAAT AGACCTGCAA AGTATAAAGA TGCCAATATC
    ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA CATGTCAGGA AACAAAAGGC CCTGGAAGCA
    GAATTAGATG CTCAGCATTT ATCAGAAACT TTTGACAATA TTGACAATTT AAGTCCCAAG
    GCATCTCATC GTAGCAAGCA GAGACACAAG CAAAATCTCT ATGGTGACTA TGTTTTTGAC
    ACCAATCGAC ATGATGATAA CAGGTCAGAC AATTTTAATA CTGGAAACAT GACTGTCCTA
    TCACCGTACT TAAACACTAC AGTATTGCCC AGCTCTTCTT CATCAAGGGG AAGTTTAGAT
    AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG GAGAGAGAAC GAGGTATTAG CCTAGGCAAC
    TATCACCCAG CAACAGAAAA CCCAGGAACC TCTTCAAAGC GAGGTTTGCA GATTTCTACC
    ACTGCAGCCC AGATTGCCAA AGTCATGGAA GAAGTATCAG CTATTCATAC CTCTCAGGAA
    GACAGAAGTT CTGGGTCTAC CACAGAACTA CACTGTGGGA CAGATGAGAG GAATGCACTA
    AGAAGAAGCT CTACCACCCA CACACATGCA AACACGTACA ACTTTACCAA GTCAGAAAAC
    TCAAACAGAA CATGTCCAAT ACCATATGCC AAAGTAGAAT ATAAGAGATC TTCAAATGAT
    AGTTTAAATA GTGTCAGCAG TAGTGATGGT TATGGTAAAA GAGGTCAGAT GAAACCTTCA
    ATTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GCTATGGTCA GTATCCAGCT
    GACCTAGCCC ATAAAATACA TAGTGCAAAT CATATGGATG ATAATGATGG AGAACTAGAT
    ACACCAATAA ATTACAGTCT CAAATATTCC GATGAACAGT TGAACTCTGG GAGGCAGAGC
    CCTTCACAGA ATGAAAGATG GGCAAGACCC AAACATATAC TAGAAGATGA AATAAAACCG
    AATGAGCAGA GACAATCAAG GAGTCAAAGC ACAGCTTATC CCGTGTATCC TGAGAGCACT
    GATGATAAAC ACCTCAAGTT CCAACCACAC TTTGGGCAGC AAGAATGTGT TTCCCCATAC
    AGGTCAAGAG CAGCCAATGG ATCTGAAACA AATCGAGTAG GCTCTAATCA TGGAATTAGT
    CAAAATGTGA ACCAGTCTTT GTGTCAAGAA GATGACTATG AAGACGATAA ACCAACCAAC
    TATAGCGAAC GTTACTCTGA GGAAGGGCAG CATGAGGAAG AAGAGAGGCC AACCAATTAT
    AGCATAAAAT ACAGTGAAGA AAAACATCAC GTGGATCAGC CTATTGATTA TAGTTTAAAA
    TACACCACAG ACATTCCTTC TTCACAGAAA CCAGCATTTT CATTCTCAAA GAATTCATCT
    GGACAGAGCA CAAAAACTGA ACACATCTCT TCAAGCAGCG AGAATACATC CACAACTTCA
    TCTAATGCCA AGAGGCAGAA TCAGCTGCAT CCAAGCTCAG CACAGAGCAG AAGTGGTCAG
    ACCCCAAAAG CCACCTCTTC CTCTTGCAAA GTCCCCTCTA TCAACCAAGA AACAATACAG
    ACTTACTGTG TAGAAGATAC CCCAATATGT TTTTCAAGAT GCAGTTCATT ATCATCTCTG
    TCATCTGCTG AAGATGAAGT AGGGTGTGAT CAGACAACAC AAGAAGCAGA GTCTGCTAAC
    ACTCTGCAAA TAGCTGAAAT CAAGGACAAC AGCGGACCTA GGTCAAATGA AGATTCTGTG
    AGTAAAGTTC CAGCAGGGTC ACAGCACATT AGAACCAAAT CCAGCAGACT CCAGGCTTCT
    GGTCTGTCTT CAGAGTCAGC CAGGCACAAA GCTGTTGAAT TTTCTTCAGG GGCCAAATCT
    CCTTCCAAGA GCGGTGCTCA GACACCCAAA AGTCCACCAG AGCACTACGT TCAGGAGACC
    CCACTCATGT TTAGCAGATG TACTTCAGTC AGTTCACTCG ACAGTTTTGA GAGTCGCTCG
    ATTGCCAGCT CTGTTCAGAG TGAACCCTGC AGTGGAATGG TAAGTGGCAT CATAAGCCCC
    AGTGACCTCC CAGATAGCCC TGGACAAACC ATGCCACCAA GCAGAAGCAA AACCCCTCCG
    CCACCGCCTC CGCCTCCTCC TCAGACAGTT CAAACGAAGC AGGAAGTACC TAAAAATAAA
    GCACCTAGTG CTGAAAAGAG AGAAAGTGGA CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA
    CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT ACTCTGTTAC ATTTTGCCAC AGAGAGTACT
    CCTGATGGAT TTTCTTGTTC ATCTAGCCTG AGCGCTCTGA GCCTTGATGA GCCATTTATT
    CAGAAAGATG TGGAACTAAG AATAATGCCT CCAGTTCAGG AAAATGACAA CGGGAATGAA
    ACAGAAAGTG AGCAGCCTGA AGAATCAAAT GAAAACCAGG AAAAAGAGGC AGAAAAACCC
    ACTGATTCTG AAAAAGATCT TTTAGATGAG TCAGATGATG ATGATATTGA AATACTAGAA
    GAGTGTATAA TTTCTGCCAT GCCAACAAAA TCTTCACGCA AAGCCAAAAA ACCAGCCCAG
    ACTACTTCAA AATTACCTCC ACCTGTGGCA AGGAAACCAA GTCAGCTCCC TGTATACAAA
    CTTCTGCCAT CGCAAAACAG GTTACAGGCA CAAAAGCATG TTAGTTTTAC ACCAGGAGAT
    GATATGCCAC GGGTGTATTG TGTAGAAGGG ACACCTATAA ACTTTTCCAC AGCTACATCT
    CTGAGTGATC TAACGATAGA ATCCCCTCCA AATGAGTTAG CTGCTGGAGA AGGGGTTAGA
    GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA CGAGATACCA TTCCTACTGA AGGCAGAAGT
    ACAGATGAGG CTCAACGAGG GAAAGCCTCA TCTGTCACTG TGCCTGAACT GGACGACAGC
    AAAACAGAAG AAGGTGATAT TCTTGCAGAA TGCATTAATT CTGCCATGCC CAAAGGAAAA
    AGTCACAAGC CTTTCCGTGT GAAAAAGATC ATGGACCAGG TCCAGCAAGC ATCTATGTCT
    TCATCTGGAA CTAACAAAAA TCAATTAGAT GGTAAGACGA AGAAACCTAC TTCACCAGTA
    AAACCTATAC CACAAAATAC TGAATACAGG ACACGTGTAA GAAAAAATAC AGACTCAAAA
    AATAATCTAA ATGCAGAAAG AAATTTCTCG GAAAACAAAG ACTCAAAGAA ACAGCACTTG
    AAAAATAATT CCAAGGACTT CAATGATAAA CTGCCAAATA ATGAAGACAG AGTCAGAGGA
    AGTTTTACTT TTGATTCACC CCATCATTAC ACGCCCATTG AAGGCACTCC GTACTGTTTT
    TCACGAAATG ACTCTCTGAG TTCTCTAGAT TTTGATGATG ATGATGTTGA CCTTTCCAGG
    GAAAAGGCTG AATTAAGAAA GGGGAAGGAA AATAAAGAAT CAGAAGCTAA AGTGACCAAC
    CACACAGAAC TAACCTCAAA CCAACAATCA GCTAGTAAGA CACCAGCTGT TACAAAGCAG
    CCAATAAATA GAGGTCAGTC TAAACCCGTG CTGCAGAAGC AGTCCACTTT TCCCCAGTCT
    TCCAAAGATA TACCAGACAG AGGGGCAGCA ACAGATGAGA AATTACAGAA TTTCGCTATT
    GAAAATACTC CAGTTTGCTT TTCTCGAAAT TCCTCTCTAA GCTCTCTTAG TGACATTGAT
    CAAGAAAACA ACAACAACAA GGAAAATGAA CCTGTCAAAG AGACAGAGCC CCCTGCCTCA
    CAGGGAGAAC CAGGTAAACC CCAGGCCTCA GGTTATGCTC CTAAATCGTT TCACGTGGAA
    GACACCCCTG TTTGTTTCTC AAGAAACAGT TCTCTCAGTT CTCTTAGTAT TGATTCTGAA
    GATGACCTGC TGCAGGAATG TATAAGTTCT GCAATGCCAA AAAAGAAAAA GCCTTCAAGG
    CTCAAGCCCG ATAATGAAAA GCATAGTCCC AGAAATATGG GTGGCATATT AGCAGAAGAT
    TTGACACTCG ATTTGAAAGA TATACAGAGA CCAGATTCAG AACATGGTTT ATCCCCAGAT
    TCAGAAAATT TTGATTGGAA AGCTATTCAG GAAGGTGCAA ATTCCATAGT AAGTAGTTTA
    CATCAAGCTG CTGCCGCTGC ATGTTTATCT CGACAAGCTT CATCTGATTC AGATTCTATC
    CTTTCATTGA AATCGGGTAT CTCTCTGGGA TCACCATTTC ACCTTACACC TGATCAAGAA
    GAAAAACCCT TTACGAGTAA TAAAGGTCCA CGAATTCTAA AACCTGGGGA GAAAAGTACA
    TTGGAAACTA AAAAAATAGA ATCTGAAAAT AAAGGAATAA AAGGAGGCAA AAAGGTTTAT
    AAAAGTTTGA TTACTGGAAA AGTTCGATCT AATTCGGAAA TTTCGAGCCA AATGAAACAA
    CCCCTTCAAA CAAACATGCC TTCAATCTCT CGAGGTAGGA CAATGATTCA TATTCCAGGA
    GTTCGGAATA GCTCTTCAAG TACAAGCCCA GTTTCTAAAA AAGGCCCGCC CCTCAAGACT
    CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG CCGGCTACCA CCTCTCCCAG AGGAACCAAG
    CCATCAGTGA AGTCAGAATT AAGCCCTGTT ACAAGGCAGG CATCCCAGAC AGCAGGATCA
    AACAAAGGAC CTTCTAGATC AGGATCTAGA GATTCCACTC CTTCAAGACC TGCCCAGCAA
    CCATTAAGTA GACCAATGCA GTCTCCAGGG CGAAACTCAA TCTCTCCTGG TAGAAATGGA
    ATAAGTCCCC CTAACAAATT ATCTCAACTA CCAAGGACGT CATCCCCTAG TACTGCTTCA
    ACTAAGTCCT CAGGTTCTGG GAAAATGTCT TACACATCTC CTGGCAGACA GATGAGCCAG
    CAGAACCTCA CCAAACAAAC AGGCTTATCC AAGAATGGCA GTGGTATCCC AAGAAGTGAA
    TCTGCCTCCA AAGGGCTAAA TCAAATGAGT AATAGTAATG GATCCAATAA AAAAGTAGAA
    CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT GGAAGTGAAT CCGATAGGTC AGAGAGACCT
    GTATTAGTAC GCCAATCAAC TTTCATCAAA GAAGCTCCAA GCCCAACCCT AAGGAGAAAA
    CTGGAGGAAT CTGCTTCATT TGAATCTCTT TCTCCATCTT CCAGACCCGA TTCTCCCACA
    CGGTCCCAGG CTCATACTCC AGTTTTAAGT CCTTCCCTTC CTGATATGTC TCTATCTACA
    CATTCGTCTC TTCAGTCTGG TGGATGGCGA AAACTCCCAC CTAACCTCAG TCCCACCATA
    GAGTATAATG ATGGAAGACC AGTAAAGCGC CATGATATAG CACGCTCTCA TTCCGAAAGT
    CCTTCCAGAC TTCCCATCAA TAGGTCAGGG ACCTGGAAAC GTGAGCACAG CAAACACTCA
    TCATCACTTC CTCGAGTAAG CACTTGGAGA AGAACTGGAA GTTCATCCTC AATTCTTTCT
    GCTTCATCAG AATCTAGTGA AAAGGCAAAA AGTGAGGACG AAAAACAAGT GAACTCTATT
    TCAGGAAGCA AACAAACTAA AGAAAACCAG GTATCCACAA AAGGAACATG GAGAAAAATA
    AAAGAAAGTG AAATTTCTCC CACAAATAGT ACTTCTCAGA CCACTTCTTC AGGTGCTGCA
    AATGGTGCTG AATCAAAGAC TCTGATTTAT CAAATGGCAC CTGCTGTTTC TAAAACAGAG
    GATGTTTGGG TGAGAATTGA GGATTGCCCC ATTAACAACC CTAGATCTGG AAGATCTCCA
    ACAGGAAATA CTCCCCCCGT GATTGACACT GTTTCAGAAA AGGGAAACCC GAACCCTAAA
    GATTCAAAAG ATAATCAGGG AAAACAAAAT GTGAGCAATG GTAGTGCCCC TACACGCACC
    ATGGGTCTGG AAAACCGCCT GAATTCCTTT ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA
    ACTGAGACAA AACCGGGACA CAGTAATAAC CCTGTCCCTG CATCCGAGAC TAGTGAAAGT
    TCTATAGCCG AGCGTACCCC GTTTAGTTCT AGCAGCTCAA GCAAGCACAG TTCACCAAGT
    GGGACTGTCG CCGCCAGGGT GAGTCCTTTT AATTACAACC CAAGCCCAAG GAAGAGCAGC
    ACAGATGGCA CTTCAGCCCG ACCGTCTCAG ATCCCAACGC CAGTGAGCAA CAACACAAAG
    AAACGCGACT CAAAACCCGA CAGCACAGAA CCCAGCGGGA CTCAAAGTCC TAAACGCCAT
    TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853408.1
    CDS329..8938
    Translation

    Target ORF information:

    RefSeq Version XM_024997640.1
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGT AGGAAGGCTA CCCGGGGCAT TTCATCCCAG
    GAGCTAGGGC AGGGGCTTTC AGGGGGACTA CAGGCCATTG CAGAATTATT GCAAGTGGAC
    TGTGAAATGT ATGGGCTTAC TAATGACCAC TACAGTATTA CTTTAAGACG ATATGCAGGA
    ATGGCTTTGA CAAACTTGAC TTTCGGAGAT GTAGCCAACA AGGCTACTCT CTGCTCTATG
    AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG
    GTTATTGCGA GTGTTTTGAG GAATCTGTCT TGGAGAGCAG ATGTAAATAG TAAAAAGACT
    TTGCGTGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT GAAAAAGGAA
    TCAACCCTCA AAAGTGTATT GAGTGCCTTA TGGAACTTGT CAGCACACTG CACTGAGAAT
    AAAGCTGACA TATGTGCTGT GGATGGTGCG CTTGCATTTT TGGTTGGCAC TCTCACTTAT
    CGCAGCCAGA CCAATACTTT AGCCATTATT GAAAGTGGAG GTGGTATATT ACGGAATGTA
    TCCAGCTTGA TAGCTACAAA TGAGGACCAC AGGCAAATCC TAAGAGAGAA TAATTGCTTA
    CAGACCTTAT TACAACACTT GAAATCTCAC AGTTTGACAA TAGTCAGCAA TGCATGCGGA
    ACCTTGTGGA ATCTCTCAGC AAGAAACCCT AAAGACCAGG AAGCATTGTG GGACATGGGA
    GCAGTCAGCA TGCTCAAGAA CCTCATTCAT TCAAAGCACA AGATGATTGC TATGGGAAGT
    GCTGCAGCTT TAAGGAATCT CATGGCAAAT AGACCTGCAA AGTATAAAGA TGCCAATATC
    ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA CATGTCAGGA AACAAAAGGC CCTGGAAGCA
    GAATTAGATG CTCAGCATTT ATCAGAAACT TTTGACAATA TTGACAATTT AAGTCCCAAG
    GCATCTCATC GTAGCAAGCA GAGACACAAG CAAAATCTCT ATGGTGACTA TGTTTTTGAC
    ACCAATCGAC ATGATGATAA CAGGTCAGAC AATTTTAATA CTGGAAACAT GACTGTCCTA
    TCACCGTACT TAAACACTAC AGTATTGCCC AGCTCTTCTT CATCAAGGGG AAGTTTAGAT
    AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG GAGAGAGAAC GAGGTATTAG CCTAGGCAAC
    TATCACCCAG CAACAGAAAA CCCAGGAACC TCTTCAAAGC GAGGTTTGCA GATTTCTACC
    ACTGCAGCCC AGATTGCCAA AGTCATGGAA GAAGTATCAG CTATTCATAC CTCTCAGGAA
    GACAGAAGTT CTGGGTCTAC CACAGAACTA CACTGTGGGA CAGATGAGAG GAATGCACTA
    AGAAGAAGCT CTACCACCCA CACACATGCA AACACGTACA ACTTTACCAA GTCAGAAAAC
    TCAAACAGAA CATGTCCAAT ACCATATGCC AAAGTAGAAT ATAAGAGATC TTCAAATGAT
    AGTTTAAATA GTGTCAGCAG TAGTGATGGT TATGGTAAAA GAGGTCAGAT GAAACCTTCA
    ATTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GCTATGGTCA GTATCCAGCT
    GACCTAGCCC ATAAAATACA TAGTGCAAAT CATATGGATG ATAATGATGG AGAACTAGAT
    ACACCAATAA ATTACAGTCT CAAATATTCC GATGAACAGT TGAACTCTGG GAGGCAGAGC
    CCTTCACAGA ATGAAAGATG GGCAAGACCC AAACATATAC TAGAAGATGA AATAAAACCG
    AATGAGCAGA GACAATCAAG GAGTCAAAGC ACAGCTTATC CCGTGTATCC TGAGAGCACT
    GATGATAAAC ACCTCAAGTT CCAACCACAC TTTGGGCAGC AAGAATGTGT TTCCCCATAC
    AGGTCAAGAG CAGCCAATGG ATCTGAAACA AATCGAGTAG GCTCTAATCA TGGAATTAGT
    CAAAATGTGA ACCAGTCTTT GTGTCAAGAA GATGACTATG AAGACGATAA ACCAACCAAC
    TATAGCGAAC GTTACTCTGA GGAAGGGCAG CATGAGGAAG AAGAGAGGCC AACCAATTAT
    AGCATAAAAT ACAGTGAAGA AAAACATCAC GTGGATCAGC CTATTGATTA TAGTTTAAAA
    TACACCACAG ACATTCCTTC TTCACAGAAA CCAGCATTTT CATTCTCAAA GAATTCATCT
    GGACAGAGCA CAAAAACTGA ACACATCTCT TCAAGCAGCG AGAATACATC CACAACTTCA
    TCTAATGCCA AGAGGCAGAA TCAGCTGCAT CCAAGCTCAG CACAGAGCAG AAGTGGTCAG
    ACCCCAAAAG CCACCTCTTC CTCTTGCAAA GTCCCCTCTA TCAACCAAGA AACAATACAG
    ACTTACTGTG TAGAAGATAC CCCAATATGT TTTTCAAGAT GCAGTTCATT ATCATCTCTG
    TCATCTGCTG AAGATGAAGT AGGGTGTGAT CAGACAACAC AAGAAGCAGA GTCTGCTAAC
    ACTCTGCAAA TAGCTGAAAT CAAGGACAAC AGCGGACCTA GGTCAAATGA AGATTCTGTG
    AGTAAAGTTC CAGCAGGGTC ACAGCACATT AGAACCAAAT CCAGCAGACT CCAGGCTTCT
    GGTCTGTCTT CAGAGTCAGC CAGGCACAAA GCTGTTGAAT TTTCTTCAGG GGCCAAATCT
    CCTTCCAAGA GCGGTGCTCA GACACCCAAA AGTCCACCAG AGCACTACGT TCAGGAGACC
    CCACTCATGT TTAGCAGATG TACTTCAGTC AGTTCACTCG ACAGTTTTGA GAGTCGCTCG
    ATTGCCAGCT CTGTTCAGAG TGAACCCTGC AGTGGAATGG TAAGTGGCAT CATAAGCCCC
    AGTGACCTCC CAGATAGCCC TGGACAAACC ATGCCACCAA GCAGAAGCAA AACCCCTCCG
    CCACCGCCTC CGCCTCCTCC TCAGACAGTT CAAACGAAGC AGGAAGTACC TAAAAATAAA
    GCACCTAGTG CTGAAAAGAG AGAAAGTGGA CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA
    CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT ACTCTGTTAC ATTTTGCCAC AGAGAGTACT
    CCTGATGGAT TTTCTTGTTC ATCTAGCCTG AGCGCTCTGA GCCTTGATGA GCCATTTATT
    CAGAAAGATG TGGAACTAAG AATAATGCCT CCAGTTCAGG AAAATGACAA CGGGAATGAA
    ACAGAAAGTG AGCAGCCTGA AGAATCAAAT GAAAACCAGG AAAAAGAGGC AGAAAAACCC
    ACTGATTCTG AAAAAGATCT TTTAGATGAG TCAGATGATG ATGATATTGA AATACTAGAA
    GAGTGTATAA TTTCTGCCAT GCCAACAAAA TCTTCACGCA AAGCCAAAAA ACCAGCCCAG
    ACTACTTCAA AATTACCTCC ACCTGTGGCA AGGAAACCAA GTCAGCTCCC TGTATACAAA
    CTTCTGCCAT CGCAAAACAG GTTACAGGCA CAAAAGCATG TTAGTTTTAC ACCAGGAGAT
    GATATGCCAC GGGTGTATTG TGTAGAAGGG ACACCTATAA ACTTTTCCAC AGCTACATCT
    CTGAGTGATC TAACGATAGA ATCCCCTCCA AATGAGTTAG CTGCTGGAGA AGGGGTTAGA
    GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA CGAGATACCA TTCCTACTGA AGGCAGAAGT
    ACAGATGAGG CTCAACGAGG GAAAGCCTCA TCTGTCACTG TGCCTGAACT GGACGACAGC
    AAAACAGAAG AAGGTGATAT TCTTGCAGAA TGCATTAATT CTGCCATGCC CAAAGGAAAA
    AGTCACAAGC CTTTCCGTGT GAAAAAGATC ATGGACCAGG TCCAGCAAGC ATCTATGTCT
    TCATCTGGAA CTAACAAAAA TCAATTAGAT GGTAAGACGA AGAAACCTAC TTCACCAGTA
    AAACCTATAC CACAAAATAC TGAATACAGG ACACGTGTAA GAAAAAATAC AGACTCAAAA
    AATAATCTAA ATGCAGAAAG AAATTTCTCG GAAAACAAAG ACTCAAAGAA ACAGCACTTG
    AAAAATAATT CCAAGGACTT CAATGATAAA CTGCCAAATA ATGAAGACAG AGTCAGAGGA
    AGTTTTACTT TTGATTCACC CCATCATTAC ACGCCCATTG AAGGCACTCC GTACTGTTTT
    TCACGAAATG ACTCTCTGAG TTCTCTAGAT TTTGATGATG ATGATGTTGA CCTTTCCAGG
    GAAAAGGCTG AATTAAGAAA GGGGAAGGAA AATAAAGAAT CAGAAGCTAA AGTGACCAAC
    CACACAGAAC TAACCTCAAA CCAACAATCA GCTAGTAAGA CACCAGCTGT TACAAAGCAG
    CCAATAAATA GAGGTCAGTC TAAACCCGTG CTGCAGAAGC AGTCCACTTT TCCCCAGTCT
    TCCAAAGATA TACCAGACAG AGGGGCAGCA ACAGATGAGA AATTACAGAA TTTCGCTATT
    GAAAATACTC CAGTTTGCTT TTCTCGAAAT TCCTCTCTAA GCTCTCTTAG TGACATTGAT
    CAAGAAAACA ACAACAACAA GGAAAATGAA CCTGTCAAAG AGACAGAGCC CCCTGCCTCA
    CAGGGAGAAC CAGGTAAACC CCAGGCCTCA GGTTATGCTC CTAAATCGTT TCACGTGGAA
    GACACCCCTG TTTGTTTCTC AAGAAACAGT TCTCTCAGTT CTCTTAGTAT TGATTCTGAA
    GATGACCTGC TGCAGGAATG TATAAGTTCT GCAATGCCAA AAAAGAAAAA GCCTTCAAGG
    CTCAAGCCCG ATAATGAAAA GCATAGTCCC AGAAATATGG GTGGCATATT AGCAGAAGAT
    TTGACACTCG ATTTGAAAGA TATACAGAGA CCAGATTCAG AACATGGTTT ATCCCCAGAT
    TCAGAAAATT TTGATTGGAA AGCTATTCAG GAAGGTGCAA ATTCCATAGT AAGTAGTTTA
    CATCAAGCTG CTGCCGCTGC ATGTTTATCT CGACAAGCTT CATCTGATTC AGATTCTATC
    CTTTCATTGA AATCGGGTAT CTCTCTGGGA TCACCATTTC ACCTTACACC TGATCAAGAA
    GAAAAACCCT TTACGAGTAA TAAAGGTCCA CGAATTCTAA AACCTGGGGA GAAAAGTACA
    TTGGAAACTA AAAAAATAGA ATCTGAAAAT AAAGGAATAA AAGGAGGCAA AAAGGTTTAT
    AAAAGTTTGA TTACTGGAAA AGTTCGATCT AATTCGGAAA TTTCGAGCCA AATGAAACAA
    CCCCTTCAAA CAAACATGCC TTCAATCTCT CGAGGTAGGA CAATGATTCA TATTCCAGGA
    GTTCGGAATA GCTCTTCAAG TACAAGCCCA GTTTCTAAAA AAGGCCCGCC CCTCAAGACT
    CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG CCGGCTACCA CCTCTCCCAG AGGAACCAAG
    CCATCAGTGA AGTCAGAATT AAGCCCTGTT ACAAGGCAGG CATCCCAGAC AGCAGGATCA
    AACAAAGGAC CTTCTAGATC AGGATCTAGA GATTCCACTC CTTCAAGACC TGCCCAGCAA
    CCATTAAGTA GACCAATGCA GTCTCCAGGG CGAAACTCAA TCTCTCCTGG TAGAAATGGA
    ATAAGTCCCC CTAACAAATT ATCTCAACTA CCAAGGACGT CATCCCCTAG TACTGCTTCA
    ACTAAGTCCT CAGGTTCTGG GAAAATGTCT TACACATCTC CTGGCAGACA GATGAGCCAG
    CAGAACCTCA CCAAACAAAC AGGCTTATCC AAGAATGGCA GTGGTATCCC AAGAAGTGAA
    TCTGCCTCCA AAGGGCTAAA TCAAATGAGT AATAGTAATG GATCCAATAA AAAAGTAGAA
    CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT GGAAGTGAAT CCGATAGGTC AGAGAGACCT
    GTATTAGTAC GCCAATCAAC TTTCATCAAA GAAGCTCCAA GCCCAACCCT AAGGAGAAAA
    CTGGAGGAAT CTGCTTCATT TGAATCTCTT TCTCCATCTT CCAGACCCGA TTCTCCCACA
    CGGTCCCAGG CTCATACTCC AGTTTTAAGT CCTTCCCTTC CTGATATGTC TCTATCTACA
    CATTCGTCTC TTCAGTCTGG TGGATGGCGA AAACTCCCAC CTAACCTCAG TCCCACCATA
    GAGTATAATG ATGGAAGACC AGTAAAGCGC CATGATATAG CACGCTCTCA TTCCGAAAGT
    CCTTCCAGAC TTCCCATCAA TAGGTCAGGG ACCTGGAAAC GTGAGCACAG CAAACACTCA
    TCATCACTTC CTCGAGTAAG CACTTGGAGA AGAACTGGAA GTTCATCCTC AATTCTTTCT
    GCTTCATCAG AATCTAGTGA AAAGGCAAAA AGTGAGGACG AAAAACAAGT GAACTCTATT
    TCAGGAAGCA AACAAACTAA AGAAAACCAG GTATCCACAA AAGGAACATG GAGAAAAATA
    AAAGAAAGTG AAATTTCTCC CACAAATAGT ACTTCTCAGA CCACTTCTTC AGGTGCTGCA
    AATGGTGCTG AATCAAAGAC TCTGATTTAT CAAATGGCAC CTGCTGTTTC TAAAACAGAG
    GATGTTTGGG TGAGAATTGA GGATTGCCCC ATTAACAACC CTAGATCTGG AAGATCTCCA
    ACAGGAAATA CTCCCCCCGT GATTGACACT GTTTCAGAAA AGGGAAACCC GAACCCTAAA
    GATTCAAAAG ATAATCAGGG AAAACAAAAT GTGAGCAATG GTAGTGCCCC TACACGCACC
    ATGGGTCTGG AAAACCGCCT GAATTCCTTT ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA
    ACTGAGACAA AACCGGGACA CAGTAATAAC CCTGTCCCTG CATCCGAGAC TAGTGAAAGT
    TCTATAGCCG AGCGTACCCC GTTTAGTTCT AGCAGCTCAA GCAAGCACAG TTCACCAAGT
    GGGACTGTCG CCGCCAGGGT GAGTCCTTTT AATTACAACC CAAGCCCAAG GAAGAGCAGC
    ACAGATGGCA CTTCAGCCCG ACCGTCTCAG ATCCCAACGC CAGTGAGCAA CAACACAAAG
    AAACGCGACT CAAAACCCGA CAGCACAGAA CCCAGCGGGA CTCAAAGTCC TAAACGCCAT
    TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219615
    Clone ID Related Accession (Same CDS sequence) XM_005211196.4
    Accession Version XM_005211196.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211196.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGG
    GGACTACAGG CCATTGCAGA ATTATTGCAA GTGGACTGTG AAATGTATGG GCTTACTAAT
    GACCACTACA GTATTACTTT AAGACGATAT GCAGGAATGG CTTTGACAAA CTTGACTTTC
    GGAGATGTAG CCAACAAGGC TACTCTCTGC TCTATGAAAG GCTGCATGAG AGCGCTCGTG
    GCTCAGCTCC AGTCTGAAAG CGAGGACTTG CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT
    CTGTCTTGGA GAGCAGATGT AAATAGTAAA AAGACTTTGC GTGAAGTTGG AAGTGTGAAA
    GCATTGATGG AATGTGCTTT GGAAGTGAAA AAGGAATCAA CCCTCAAAAG TGTATTGAGT
    GCCTTATGGA ACTTGTCAGC ACACTGCACT GAGAATAAAG CTGACATATG TGCTGTGGAT
    GGTGCGCTTG CATTTTTGGT TGGCACTCTC ACTTATCGCA GCCAGACCAA TACTTTAGCC
    ATTATTGAAA GTGGAGGTGG TATATTACGG AATGTATCCA GCTTGATAGC TACAAATGAG
    GACCACAGGC AAATCCTAAG AGAGAATAAT TGCTTACAGA CCTTATTACA ACACTTGAAA
    TCTCACAGTT TGACAATAGT CAGCAATGCA TGCGGAACCT TGTGGAATCT CTCAGCAAGA
    AACCCTAAAG ACCAGGAAGC ATTGTGGGAC ATGGGAGCAG TCAGCATGCT CAAGAACCTC
    ATTCATTCAA AGCACAAGAT GATTGCTATG GGAAGTGCTG CAGCTTTAAG GAATCTCATG
    GCAAATAGAC CTGCAAAGTA TAAAGATGCC AATATCATGT CTCCTGGTTC AAGTTTGCCT
    TCTCTACATG TCAGGAAACA AAAGGCCCTG GAAGCAGAAT TAGATGCTCA GCATTTATCA
    GAAACTTTTG ACAATATTGA CAATTTAAGT CCCAAGGCAT CTCATCGTAG CAAGCAGAGA
    CACAAGCAAA ATCTCTATGG TGACTATGTT TTTGACACCA ATCGACATGA TGATAACAGG
    TCAGACAATT TTAATACTGG AAACATGACT GTCCTATCAC CGTACTTAAA CACTACAGTA
    TTGCCCAGCT CTTCTTCATC AAGGGGAAGT TTAGATAGCT CTCGTTCTGA GAAAGATAGA
    AGTCTGGAGA GAGAACGAGG TATTAGCCTA GGCAACTATC ACCCAGCAAC AGAAAACCCA
    GGAACCTCTT CAAAGCGAGG TTTGCAGATT TCTACCACTG CAGCCCAGAT TGCCAAAGTC
    ATGGAAGAAG TATCAGCTAT TCATACCTCT CAGGAAGACA GAAGTTCTGG GTCTACCACA
    GAACTACACT GTGGGACAGA TGAGAGGAAT GCACTAAGAA GAAGCTCTAC CACCCACACA
    CATGCAAACA CGTACAACTT TACCAAGTCA GAAAACTCAA ACAGAACATG TCCAATACCA
    TATGCCAAAG TAGAATATAA GAGATCTTCA AATGATAGTT TAAATAGTGT CAGCAGTAGT
    GATGGTTATG GTAAAAGAGG TCAGATGAAA CCTTCAATTG AATCCTATTC TGAAGATGAT
    GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT CCAGCTGACC TAGCCCATAA AATACATAGT
    GCAAATCATA TGGATGATAA TGATGGAGAA CTAGATACAC CAATAAATTA CAGTCTCAAA
    TATTCCGATG AACAGTTGAA CTCTGGGAGG CAGAGCCCTT CACAGAATGA AAGATGGGCA
    AGACCCAAAC ATATACTAGA AGATGAAATA AAACCGAATG AGCAGAGACA ATCAAGGAGT
    CAAAGCACAG CTTATCCCGT GTATCCTGAG AGCACTGATG ATAAACACCT CAAGTTCCAA
    CCACACTTTG GGCAGCAAGA ATGTGTTTCC CCATACAGGT CAAGAGCAGC CAATGGATCT
    GAAACAAATC GAGTAGGCTC TAATCATGGA ATTAGTCAAA ATGTGAACCA GTCTTTGTGT
    CAAGAAGATG ACTATGAAGA CGATAAACCA ACCAACTATA GCGAACGTTA CTCTGAGGAA
    GGGCAGCATG AGGAAGAAGA GAGGCCAACC AATTATAGCA TAAAATACAG TGAAGAAAAA
    CATCACGTGG ATCAGCCTAT TGATTATAGT TTAAAATACA CCACAGACAT TCCTTCTTCA
    CAGAAACCAG CATTTTCATT CTCAAAGAAT TCATCTGGAC AGAGCACAAA AACTGAACAC
    ATCTCTTCAA GCAGCGAGAA TACATCCACA ACTTCATCTA ATGCCAAGAG GCAGAATCAG
    CTGCATCCAA GCTCAGCACA GAGCAGAAGT GGTCAGACCC CAAAAGCCAC CTCTTCCTCT
    TGCAAAGTCC CCTCTATCAA CCAAGAAACA ATACAGACTT ACTGTGTAGA AGATACCCCA
    ATATGTTTTT CAAGATGCAG TTCATTATCA TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG
    TGTGATCAGA CAACACAAGA AGCAGAGTCT GCTAACACTC TGCAAATAGC TGAAATCAAG
    GACAACAGCG GACCTAGGTC AAATGAAGAT TCTGTGAGTA AAGTTCCAGC AGGGTCACAG
    CACATTAGAA CCAAATCCAG CAGACTCCAG GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG
    CACAAAGCTG TTGAATTTTC TTCAGGGGCC AAATCTCCTT CCAAGAGCGG TGCTCAGACA
    CCCAAAAGTC CACCAGAGCA CTACGTTCAG GAGACCCCAC TCATGTTTAG CAGATGTACT
    TCAGTCAGTT CACTCGACAG TTTTGAGAGT CGCTCGATTG CCAGCTCTGT TCAGAGTGAA
    CCCTGCAGTG GAATGGTAAG TGGCATCATA AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA
    CAAACCATGC CACCAAGCAG AAGCAAAACC CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG
    ACAGTTCAAA CGAAGCAGGA AGTACCTAAA AATAAAGCAC CTAGTGCTGA AAAGAGAGAA
    AGTGGACCCA AGCAAGCTGC TGTAAATGCT GCAGTACAAA GGGTCCAGGT TCTTCCAGAG
    GCTGATACTC TGTTACATTT TGCCACAGAG AGTACTCCTG ATGGATTTTC TTGTTCATCT
    AGCCTGAGCG CTCTGAGCCT TGATGAGCCA TTTATTCAGA AAGATGTGGA ACTAAGAATA
    ATGCCTCCAG TTCAGGAAAA TGACAACGGG AATGAAACAG AAAGTGAGCA GCCTGAAGAA
    TCAAATGAAA ACCAGGAAAA AGAGGCAGAA AAACCCACTG ATTCTGAAAA AGATCTTTTA
    GATGAGTCAG ATGATGATGA TATTGAAATA CTAGAAGAGT GTATAATTTC TGCCATGCCA
    ACAAAATCTT CACGCAAAGC CAAAAAACCA GCCCAGACTA CTTCAAAATT ACCTCCACCT
    GTGGCAAGGA AACCAAGTCA GCTCCCTGTA TACAAACTTC TGCCATCGCA AAACAGGTTA
    CAGGCACAAA AGCATGTTAG TTTTACACCA GGAGATGATA TGCCACGGGT GTATTGTGTA
    GAAGGGACAC CTATAAACTT TTCCACAGCT ACATCTCTGA GTGATCTAAC GATAGAATCC
    CCTCCAAATG AGTTAGCTGC TGGAGAAGGG GTTAGAGCAG GGGCACAGTC AAGTGAGTTT
    GAAAAACGAG ATACCATTCC TACTGAAGGC AGAAGTACAG ATGAGGCTCA ACGAGGGAAA
    GCCTCATCTG TCACTGTGCC TGAACTGGAC GACAGCAAAA CAGAAGAAGG TGATATTCTT
    GCAGAATGCA TTAATTCTGC CATGCCCAAA GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA
    AAGATCATGG ACCAGGTCCA GCAAGCATCT ATGTCTTCAT CTGGAACTAA CAAAAATCAA
    TTAGATGGTA AGACGAAGAA ACCTACTTCA CCAGTAAAAC CTATACCACA AAATACTGAA
    TACAGGACAC GTGTAAGAAA AAATACAGAC TCAAAAAATA ATCTAAATGC AGAAAGAAAT
    TTCTCGGAAA ACAAAGACTC AAAGAAACAG CACTTGAAAA ATAATTCCAA GGACTTCAAT
    GATAAACTGC CAAATAATGA AGACAGAGTC AGAGGAAGTT TTACTTTTGA TTCACCCCAT
    CATTACACGC CCATTGAAGG CACTCCGTAC TGTTTTTCAC GAAATGACTC TCTGAGTTCT
    CTAGATTTTG ATGATGATGA TGTTGACCTT TCCAGGGAAA AGGCTGAATT AAGAAAGGGG
    AAGGAAAATA AAGAATCAGA AGCTAAAGTG ACCAACCACA CAGAACTAAC CTCAAACCAA
    CAATCAGCTA GTAAGACACC AGCTGTTACA AAGCAGCCAA TAAATAGAGG TCAGTCTAAA
    CCCGTGCTGC AGAAGCAGTC CACTTTTCCC CAGTCTTCCA AAGATATACC AGACAGAGGG
    GCAGCAACAG ATGAGAAATT ACAGAATTTC GCTATTGAAA ATACTCCAGT TTGCTTTTCT
    CGAAATTCCT CTCTAAGCTC TCTTAGTGAC ATTGATCAAG AAAACAACAA CAACAAGGAA
    AATGAACCTG TCAAAGAGAC AGAGCCCCCT GCCTCACAGG GAGAACCAGG TAAACCCCAG
    GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC GTGGAAGACA CCCCTGTTTG TTTCTCAAGA
    AACAGTTCTC TCAGTTCTCT TAGTATTGAT TCTGAAGATG ACCTGCTGCA GGAATGTATA
    AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT TCAAGGCTCA AGCCCGATAA TGAAAAGCAT
    AGTCCCAGAA ATATGGGTGG CATATTAGCA GAAGATTTGA CACTCGATTT GAAAGATATA
    CAGAGACCAG ATTCAGAACA TGGTTTATCC CCAGATTCAG AAAATTTTGA TTGGAAAGCT
    ATTCAGGAAG GTGCAAATTC CATAGTAAGT AGTTTACATC AAGCTGCTGC CGCTGCATGT
    TTATCTCGAC AAGCTTCATC TGATTCAGAT TCTATCCTTT CATTGAAATC GGGTATCTCT
    CTGGGATCAC CATTTCACCT TACACCTGAT CAAGAAGAAA AACCCTTTAC GAGTAATAAA
    GGTCCACGAA TTCTAAAACC TGGGGAGAAA AGTACATTGG AAACTAAAAA AATAGAATCT
    GAAAATAAAG GAATAAAAGG AGGCAAAAAG GTTTATAAAA GTTTGATTAC TGGAAAAGTT
    CGATCTAATT CGGAAATTTC GAGCCAAATG AAACAACCCC TTCAAACAAA CATGCCTTCA
    ATCTCTCGAG GTAGGACAAT GATTCATATT CCAGGAGTTC GGAATAGCTC TTCAAGTACA
    AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC AAGACTCCAG CCTCCAAAAG CCCTAGTGAA
    GGTCAGCCGG CTACCACCTC TCCCAGAGGA ACCAAGCCAT CAGTGAAGTC AGAATTAAGC
    CCTGTTACAA GGCAGGCATC CCAGACAGCA GGATCAAACA AAGGACCTTC TAGATCAGGA
    TCTAGAGATT CCACTCCTTC AAGACCTGCC CAGCAACCAT TAAGTAGACC AATGCAGTCT
    CCAGGGCGAA ACTCAATCTC TCCTGGTAGA AATGGAATAA GTCCCCCTAA CAAATTATCT
    CAACTACCAA GGACGTCATC CCCTAGTACT GCTTCAACTA AGTCCTCAGG TTCTGGGAAA
    ATGTCTTACA CATCTCCTGG CAGACAGATG AGCCAGCAGA ACCTCACCAA ACAAACAGGC
    TTATCCAAGA ATGGCAGTGG TATCCCAAGA AGTGAATCTG CCTCCAAAGG GCTAAATCAA
    ATGAGTAATA GTAATGGATC CAATAAAAAA GTAGAACTTT CTAGAATGTC TTCAACAAAG
    TCAAGTGGAA GTGAATCCGA TAGGTCAGAG AGACCTGTAT TAGTACGCCA ATCAACTTTC
    ATCAAAGAAG CTCCAAGCCC AACCCTAAGG AGAAAACTGG AGGAATCTGC TTCATTTGAA
    TCTCTTTCTC CATCTTCCAG ACCCGATTCT CCCACACGGT CCCAGGCTCA TACTCCAGTT
    TTAAGTCCTT CCCTTCCTGA TATGTCTCTA TCTACACATT CGTCTCTTCA GTCTGGTGGA
    TGGCGAAAAC TCCCACCTAA CCTCAGTCCC ACCATAGAGT ATAATGATGG AAGACCAGTA
    AAGCGCCATG ATATAGCACG CTCTCATTCC GAAAGTCCTT CCAGACTTCC CATCAATAGG
    TCAGGGACCT GGAAACGTGA GCACAGCAAA CACTCATCAT CACTTCCTCG AGTAAGCACT
    TGGAGAAGAA CTGGAAGTTC ATCCTCAATT CTTTCTGCTT CATCAGAATC TAGTGAAAAG
    GCAAAAAGTG AGGACGAAAA ACAAGTGAAC TCTATTTCAG GAAGCAAACA AACTAAAGAA
    AACCAGGTAT CCACAAAAGG AACATGGAGA AAAATAAAAG AAAGTGAAAT TTCTCCCACA
    AATAGTACTT CTCAGACCAC TTCTTCAGGT GCTGCAAATG GTGCTGAATC AAAGACTCTG
    ATTTATCAAA TGGCACCTGC TGTTTCTAAA ACAGAGGATG TTTGGGTGAG AATTGAGGAT
    TGCCCCATTA ACAACCCTAG ATCTGGAAGA TCTCCAACAG GAAATACTCC CCCCGTGATT
    GACACTGTTT CAGAAAAGGG AAACCCGAAC CCTAAAGATT CAAAAGATAA TCAGGGAAAA
    CAAAATGTGA GCAATGGTAG TGCCCCTACA CGCACCATGG GTCTGGAAAA CCGCCTGAAT
    TCCTTTATTC AGGTAGATCC TCCAGACCAA AAAGGAACTG AGACAAAACC GGGACACAGT
    AATAACCCTG TCCCTGCATC CGAGACTAGT GAAAGTTCTA TAGCCGAGCG TACCCCGTTT
    AGTTCTAGCA GCTCAAGCAA GCACAGTTCA CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT
    CCTTTTAATT ACAACCCAAG CCCAAGGAAG AGCAGCACAG ATGGCACTTC AGCCCGACCG
    TCTCAGATCC CAACGCCAGT GAGCAACAAC ACAAAGAAAC GCGACTCAAA ACCCGACAGC
    ACAGAACCCA GCGGGACTCA AAGTCCTAAA CGCCATTCTG GGTCTTACCT TGTGACATCT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211253.1
    CDS132..8717
    Translation

    Target ORF information:

    RefSeq Version XM_005211196.4
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211196.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGG
    GGACTACAGG CCATTGCAGA ATTATTGCAA GTGGACTGTG AAATGTATGG GCTTACTAAT
    GACCACTACA GTATTACTTT AAGACGATAT GCAGGAATGG CTTTGACAAA CTTGACTTTC
    GGAGATGTAG CCAACAAGGC TACTCTCTGC TCTATGAAAG GCTGCATGAG AGCGCTCGTG
    GCTCAGCTCC AGTCTGAAAG CGAGGACTTG CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT
    CTGTCTTGGA GAGCAGATGT AAATAGTAAA AAGACTTTGC GTGAAGTTGG AAGTGTGAAA
    GCATTGATGG AATGTGCTTT GGAAGTGAAA AAGGAATCAA CCCTCAAAAG TGTATTGAGT
    GCCTTATGGA ACTTGTCAGC ACACTGCACT GAGAATAAAG CTGACATATG TGCTGTGGAT
    GGTGCGCTTG CATTTTTGGT TGGCACTCTC ACTTATCGCA GCCAGACCAA TACTTTAGCC
    ATTATTGAAA GTGGAGGTGG TATATTACGG AATGTATCCA GCTTGATAGC TACAAATGAG
    GACCACAGGC AAATCCTAAG AGAGAATAAT TGCTTACAGA CCTTATTACA ACACTTGAAA
    TCTCACAGTT TGACAATAGT CAGCAATGCA TGCGGAACCT TGTGGAATCT CTCAGCAAGA
    AACCCTAAAG ACCAGGAAGC ATTGTGGGAC ATGGGAGCAG TCAGCATGCT CAAGAACCTC
    ATTCATTCAA AGCACAAGAT GATTGCTATG GGAAGTGCTG CAGCTTTAAG GAATCTCATG
    GCAAATAGAC CTGCAAAGTA TAAAGATGCC AATATCATGT CTCCTGGTTC AAGTTTGCCT
    TCTCTACATG TCAGGAAACA AAAGGCCCTG GAAGCAGAAT TAGATGCTCA GCATTTATCA
    GAAACTTTTG ACAATATTGA CAATTTAAGT CCCAAGGCAT CTCATCGTAG CAAGCAGAGA
    CACAAGCAAA ATCTCTATGG TGACTATGTT TTTGACACCA ATCGACATGA TGATAACAGG
    TCAGACAATT TTAATACTGG AAACATGACT GTCCTATCAC CGTACTTAAA CACTACAGTA
    TTGCCCAGCT CTTCTTCATC AAGGGGAAGT TTAGATAGCT CTCGTTCTGA GAAAGATAGA
    AGTCTGGAGA GAGAACGAGG TATTAGCCTA GGCAACTATC ACCCAGCAAC AGAAAACCCA
    GGAACCTCTT CAAAGCGAGG TTTGCAGATT TCTACCACTG CAGCCCAGAT TGCCAAAGTC
    ATGGAAGAAG TATCAGCTAT TCATACCTCT CAGGAAGACA GAAGTTCTGG GTCTACCACA
    GAACTACACT GTGGGACAGA TGAGAGGAAT GCACTAAGAA GAAGCTCTAC CACCCACACA
    CATGCAAACA CGTACAACTT TACCAAGTCA GAAAACTCAA ACAGAACATG TCCAATACCA
    TATGCCAAAG TAGAATATAA GAGATCTTCA AATGATAGTT TAAATAGTGT CAGCAGTAGT
    GATGGTTATG GTAAAAGAGG TCAGATGAAA CCTTCAATTG AATCCTATTC TGAAGATGAT
    GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT CCAGCTGACC TAGCCCATAA AATACATAGT
    GCAAATCATA TGGATGATAA TGATGGAGAA CTAGATACAC CAATAAATTA CAGTCTCAAA
    TATTCCGATG AACAGTTGAA CTCTGGGAGG CAGAGCCCTT CACAGAATGA AAGATGGGCA
    AGACCCAAAC ATATACTAGA AGATGAAATA AAACCGAATG AGCAGAGACA ATCAAGGAGT
    CAAAGCACAG CTTATCCCGT GTATCCTGAG AGCACTGATG ATAAACACCT CAAGTTCCAA
    CCACACTTTG GGCAGCAAGA ATGTGTTTCC CCATACAGGT CAAGAGCAGC CAATGGATCT
    GAAACAAATC GAGTAGGCTC TAATCATGGA ATTAGTCAAA ATGTGAACCA GTCTTTGTGT
    CAAGAAGATG ACTATGAAGA CGATAAACCA ACCAACTATA GCGAACGTTA CTCTGAGGAA
    GGGCAGCATG AGGAAGAAGA GAGGCCAACC AATTATAGCA TAAAATACAG TGAAGAAAAA
    CATCACGTGG ATCAGCCTAT TGATTATAGT TTAAAATACA CCACAGACAT TCCTTCTTCA
    CAGAAACCAG CATTTTCATT CTCAAAGAAT TCATCTGGAC AGAGCACAAA AACTGAACAC
    ATCTCTTCAA GCAGCGAGAA TACATCCACA ACTTCATCTA ATGCCAAGAG GCAGAATCAG
    CTGCATCCAA GCTCAGCACA GAGCAGAAGT GGTCAGACCC CAAAAGCCAC CTCTTCCTCT
    TGCAAAGTCC CCTCTATCAA CCAAGAAACA ATACAGACTT ACTGTGTAGA AGATACCCCA
    ATATGTTTTT CAAGATGCAG TTCATTATCA TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG
    TGTGATCAGA CAACACAAGA AGCAGAGTCT GCTAACACTC TGCAAATAGC TGAAATCAAG
    GACAACAGCG GACCTAGGTC AAATGAAGAT TCTGTGAGTA AAGTTCCAGC AGGGTCACAG
    CACATTAGAA CCAAATCCAG CAGACTCCAG GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG
    CACAAAGCTG TTGAATTTTC TTCAGGGGCC AAATCTCCTT CCAAGAGCGG TGCTCAGACA
    CCCAAAAGTC CACCAGAGCA CTACGTTCAG GAGACCCCAC TCATGTTTAG CAGATGTACT
    TCAGTCAGTT CACTCGACAG TTTTGAGAGT CGCTCGATTG CCAGCTCTGT TCAGAGTGAA
    CCCTGCAGTG GAATGGTAAG TGGCATCATA AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA
    CAAACCATGC CACCAAGCAG AAGCAAAACC CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG
    ACAGTTCAAA CGAAGCAGGA AGTACCTAAA AATAAAGCAC CTAGTGCTGA AAAGAGAGAA
    AGTGGACCCA AGCAAGCTGC TGTAAATGCT GCAGTACAAA GGGTCCAGGT TCTTCCAGAG
    GCTGATACTC TGTTACATTT TGCCACAGAG AGTACTCCTG ATGGATTTTC TTGTTCATCT
    AGCCTGAGCG CTCTGAGCCT TGATGAGCCA TTTATTCAGA AAGATGTGGA ACTAAGAATA
    ATGCCTCCAG TTCAGGAAAA TGACAACGGG AATGAAACAG AAAGTGAGCA GCCTGAAGAA
    TCAAATGAAA ACCAGGAAAA AGAGGCAGAA AAACCCACTG ATTCTGAAAA AGATCTTTTA
    GATGAGTCAG ATGATGATGA TATTGAAATA CTAGAAGAGT GTATAATTTC TGCCATGCCA
    ACAAAATCTT CACGCAAAGC CAAAAAACCA GCCCAGACTA CTTCAAAATT ACCTCCACCT
    GTGGCAAGGA AACCAAGTCA GCTCCCTGTA TACAAACTTC TGCCATCGCA AAACAGGTTA
    CAGGCACAAA AGCATGTTAG TTTTACACCA GGAGATGATA TGCCACGGGT GTATTGTGTA
    GAAGGGACAC CTATAAACTT TTCCACAGCT ACATCTCTGA GTGATCTAAC GATAGAATCC
    CCTCCAAATG AGTTAGCTGC TGGAGAAGGG GTTAGAGCAG GGGCACAGTC AAGTGAGTTT
    GAAAAACGAG ATACCATTCC TACTGAAGGC AGAAGTACAG ATGAGGCTCA ACGAGGGAAA
    GCCTCATCTG TCACTGTGCC TGAACTGGAC GACAGCAAAA CAGAAGAAGG TGATATTCTT
    GCAGAATGCA TTAATTCTGC CATGCCCAAA GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA
    AAGATCATGG ACCAGGTCCA GCAAGCATCT ATGTCTTCAT CTGGAACTAA CAAAAATCAA
    TTAGATGGTA AGACGAAGAA ACCTACTTCA CCAGTAAAAC CTATACCACA AAATACTGAA
    TACAGGACAC GTGTAAGAAA AAATACAGAC TCAAAAAATA ATCTAAATGC AGAAAGAAAT
    TTCTCGGAAA ACAAAGACTC AAAGAAACAG CACTTGAAAA ATAATTCCAA GGACTTCAAT
    GATAAACTGC CAAATAATGA AGACAGAGTC AGAGGAAGTT TTACTTTTGA TTCACCCCAT
    CATTACACGC CCATTGAAGG CACTCCGTAC TGTTTTTCAC GAAATGACTC TCTGAGTTCT
    CTAGATTTTG ATGATGATGA TGTTGACCTT TCCAGGGAAA AGGCTGAATT AAGAAAGGGG
    AAGGAAAATA AAGAATCAGA AGCTAAAGTG ACCAACCACA CAGAACTAAC CTCAAACCAA
    CAATCAGCTA GTAAGACACC AGCTGTTACA AAGCAGCCAA TAAATAGAGG TCAGTCTAAA
    CCCGTGCTGC AGAAGCAGTC CACTTTTCCC CAGTCTTCCA AAGATATACC AGACAGAGGG
    GCAGCAACAG ATGAGAAATT ACAGAATTTC GCTATTGAAA ATACTCCAGT TTGCTTTTCT
    CGAAATTCCT CTCTAAGCTC TCTTAGTGAC ATTGATCAAG AAAACAACAA CAACAAGGAA
    AATGAACCTG TCAAAGAGAC AGAGCCCCCT GCCTCACAGG GAGAACCAGG TAAACCCCAG
    GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC GTGGAAGACA CCCCTGTTTG TTTCTCAAGA
    AACAGTTCTC TCAGTTCTCT TAGTATTGAT TCTGAAGATG ACCTGCTGCA GGAATGTATA
    AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT TCAAGGCTCA AGCCCGATAA TGAAAAGCAT
    AGTCCCAGAA ATATGGGTGG CATATTAGCA GAAGATTTGA CACTCGATTT GAAAGATATA
    CAGAGACCAG ATTCAGAACA TGGTTTATCC CCAGATTCAG AAAATTTTGA TTGGAAAGCT
    ATTCAGGAAG GTGCAAATTC CATAGTAAGT AGTTTACATC AAGCTGCTGC CGCTGCATGT
    TTATCTCGAC AAGCTTCATC TGATTCAGAT TCTATCCTTT CATTGAAATC GGGTATCTCT
    CTGGGATCAC CATTTCACCT TACACCTGAT CAAGAAGAAA AACCCTTTAC GAGTAATAAA
    GGTCCACGAA TTCTAAAACC TGGGGAGAAA AGTACATTGG AAACTAAAAA AATAGAATCT
    GAAAATAAAG GAATAAAAGG AGGCAAAAAG GTTTATAAAA GTTTGATTAC TGGAAAAGTT
    CGATCTAATT CGGAAATTTC GAGCCAAATG AAACAACCCC TTCAAACAAA CATGCCTTCA
    ATCTCTCGAG GTAGGACAAT GATTCATATT CCAGGAGTTC GGAATAGCTC TTCAAGTACA
    AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC AAGACTCCAG CCTCCAAAAG CCCTAGTGAA
    GGTCAGCCGG CTACCACCTC TCCCAGAGGA ACCAAGCCAT CAGTGAAGTC AGAATTAAGC
    CCTGTTACAA GGCAGGCATC CCAGACAGCA GGATCAAACA AAGGACCTTC TAGATCAGGA
    TCTAGAGATT CCACTCCTTC AAGACCTGCC CAGCAACCAT TAAGTAGACC AATGCAGTCT
    CCAGGGCGAA ACTCAATCTC TCCTGGTAGA AATGGAATAA GTCCCCCTAA CAAATTATCT
    CAACTACCAA GGACGTCATC CCCTAGTACT GCTTCAACTA AGTCCTCAGG TTCTGGGAAA
    ATGTCTTACA CATCTCCTGG CAGACAGATG AGCCAGCAGA ACCTCACCAA ACAAACAGGC
    TTATCCAAGA ATGGCAGTGG TATCCCAAGA AGTGAATCTG CCTCCAAAGG GCTAAATCAA
    ATGAGTAATA GTAATGGATC CAATAAAAAA GTAGAACTTT CTAGAATGTC TTCAACAAAG
    TCAAGTGGAA GTGAATCCGA TAGGTCAGAG AGACCTGTAT TAGTACGCCA ATCAACTTTC
    ATCAAAGAAG CTCCAAGCCC AACCCTAAGG AGAAAACTGG AGGAATCTGC TTCATTTGAA
    TCTCTTTCTC CATCTTCCAG ACCCGATTCT CCCACACGGT CCCAGGCTCA TACTCCAGTT
    TTAAGTCCTT CCCTTCCTGA TATGTCTCTA TCTACACATT CGTCTCTTCA GTCTGGTGGA
    TGGCGAAAAC TCCCACCTAA CCTCAGTCCC ACCATAGAGT ATAATGATGG AAGACCAGTA
    AAGCGCCATG ATATAGCACG CTCTCATTCC GAAAGTCCTT CCAGACTTCC CATCAATAGG
    TCAGGGACCT GGAAACGTGA GCACAGCAAA CACTCATCAT CACTTCCTCG AGTAAGCACT
    TGGAGAAGAA CTGGAAGTTC ATCCTCAATT CTTTCTGCTT CATCAGAATC TAGTGAAAAG
    GCAAAAAGTG AGGACGAAAA ACAAGTGAAC TCTATTTCAG GAAGCAAACA AACTAAAGAA
    AACCAGGTAT CCACAAAAGG AACATGGAGA AAAATAAAAG AAAGTGAAAT TTCTCCCACA
    AATAGTACTT CTCAGACCAC TTCTTCAGGT GCTGCAAATG GTGCTGAATC AAAGACTCTG
    ATTTATCAAA TGGCACCTGC TGTTTCTAAA ACAGAGGATG TTTGGGTGAG AATTGAGGAT
    TGCCCCATTA ACAACCCTAG ATCTGGAAGA TCTCCAACAG GAAATACTCC CCCCGTGATT
    GACACTGTTT CAGAAAAGGG AAACCCGAAC CCTAAAGATT CAAAAGATAA TCAGGGAAAA
    CAAAATGTGA GCAATGGTAG TGCCCCTACA CGCACCATGG GTCTGGAAAA CCGCCTGAAT
    TCCTTTATTC AGGTAGATCC TCCAGACCAA AAAGGAACTG AGACAAAACC GGGACACAGT
    AATAACCCTG TCCCTGCATC CGAGACTAGT GAAAGTTCTA TAGCCGAGCG TACCCCGTTT
    AGTTCTAGCA GCTCAAGCAA GCACAGTTCA CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT
    CCTTTTAATT ACAACCCAAG CCCAAGGAAG AGCAGCACAG ATGGCACTTC AGCCCGACCG
    TCTCAGATCC CAACGCCAGT GAGCAACAAC ACAAAGAAAC GCGACTCAAA ACCCGACAGC
    ACAGAACCCA GCGGGACTCA AAGTCCTAAA CGCCATTCTG GGTCTTACCT TGTGACATCT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219616
    Clone ID Related Accession (Same CDS sequence) XM_005211197.4 , XM_024997641.1
    Accession Version XM_005211197.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211197.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211254.1
    CDS329..8884
    Translation

    Target ORF information:

    RefSeq Version XM_005211197.4
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211197.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219616
    Clone ID Related Accession (Same CDS sequence) XM_005211197.4 , XM_024997641.1
    Accession Version XM_024997641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1321
    1381
    1441
    1501
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    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853409.1
    CDS93..8648
    Translation

    Target ORF information:

    RefSeq Version XM_024997641.1
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219617
    Clone ID Related Accession (Same CDS sequence) XM_015472986.2
    Accession Version XM_015472986.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGATACGAGC TTACTGTGAA
    ACCTGTTGGG AGTGGCAGGA AGCCCATGAA CAAGGGATGG ACCAGGACAA AAATCCAATG
    CCAGCTCCTG TTGAACATCA GATCTGCCCT GCTGTCTGTG TTCTAATGAA GCTTTCGTTT
    GATGAAGAGC ATAGACATGC CATGAATGAG CTTGGTAGGA AGGCTACCCG GGGCATTTCA
    TCCCAGGAGC TAGGGCAGGG GCTTTCAGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328472.1
    CDS132..8567
    Translation

    Target ORF information:

    RefSeq Version XM_015472986.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472986.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGATACGAGC TTACTGTGAA
    ACCTGTTGGG AGTGGCAGGA AGCCCATGAA CAAGGGATGG ACCAGGACAA AAATCCAATG
    CCAGCTCCTG TTGAACATCA GATCTGCCCT GCTGTCTGTG TTCTAATGAA GCTTTCGTTT
    GATGAAGAGC ATAGACATGC CATGAATGAG CTTGGTAGGA AGGCTACCCG GGGCATTTCA
    TCCCAGGAGC TAGGGCAGGG GCTTTCAGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCAC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219618
    Clone ID Related Accession (Same CDS sequence) XM_015472987.2
    Accession Version XM_015472987.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTGCCATG TTCTTCTAGA TGATCCTGAG TTAGCTAGAG GACTAAGAAG ACATGAAATT 
    TATGTCAGAT CATTGCTTCT TGCTGACCTT GACAAAGAAG AAAAGGAAAA AGACTGGTAT
    TATGCTCAAC TTCAGAATCT CACTAAAAGA ATAGATAGCC TTCCTTTAAC TGAAAATTTT
    TCCTTACAAA CAGATATGAC CAGAAGGCAG TTGGAATATG AGGCAAGGCA AATCAGAGTT
    GCAATGGAAG AACAACTAGG TACTTGCCAG GATATGGAAA AACGAGCACA GCGAAGAATA
    ACCAGAATTC AACAAATAGA AAAGGACATA CTTCGTATAC GACAGCTTTT ACAGTCCCAA
    GCAACAGAAG CAGAGAGGTC ATCTCAGAGC AAGCATGAAG CGGGCTCACA TGAAGCTGAA
    CGGCAGAATG AAGGTCAAGG AGTGGCAGAA ATCAACATGG CAACCTCGGG TAGCGGTCAG
    GTTACTGCTT CTCTGGTCCC TCTGAAGGAT GTCGGTGCAG ACTTAATCAT ACTAAAAAGG
    CCCAGGAAAG ATTCTCAAGA CTCGGGTTCT GGCTTACAGG GTTCAACTAC ACGAATAGAT
    CATGAAACAG CCAGTGTTTT GAGTTCTAGT AGCACACATT CTGCTCCTCG AAGGCTGACG
    AGTCATCTGG GAACCAAGGT GGAAATGGTG TATTCATTGT TGTCAATGCT TGGTACTCAT
    GATAAGGATG ATATGTCGCG AACTTTGCTA GCTATGTCTA GCTCCCAAGA CAGCTGTATA
    TCCATGCGAC AGTCTGGATG TCTTCCTCTC CTCATCCAGC TTTTACATGG CAATGACAAA
    GACTCTGTGT TGTTGGGAAA TTCCCGGGGC AGTAAAGAGG CTCGGGCCAG GGCCAGTGCA
    GCACTCCACA ACATCATTCA CTCACAGCCT GATGACAAGA GAGGCAGGCG TGAAATCCGA
    GTCCTTCATC TTTTGGAACA GATACGAGCT TACTGTGAAA CCTGTTGGGA GTGGCAGGAA
    GCCCATGAAC AAGGGATGGA CCAGGACAAA AATCCAATGC CAGCTCCTGT TGAACATCAG
    ATCTGCCCTG CTGTCTGTGT TCTAATGAAG CTTTCGTTTG ATGAAGAGCA TAGACATGCC
    ATGAATGAGC TTGGTAGGAA GGCTACCCGG GGCATTTCAT CCCAGGAGCT AGGGCAGGGG
    CTTTCAGGGG GACTACAGGC CATTGCAGAA TTATTGCAAG TGGACTGTGA AATGTATGGG
    CTTACTAATG ACCACTACAG TATTACTTTA AGACGATATG CAGGAATGGC TTTGACAAAC
    TTGACTTTCG GAGATGTAGC CAACAAGGCT ACTCTCTGCT CTATGAAAGG CTGCATGAGA
    GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC GAGGACTTGC AGCAGGTTAT TGCGAGTGTT
    TTGAGGAATC TGTCTTGGAG AGCAGATGTA AATAGTAAAA AGACTTTGCG TGAAGTTGGA
    AGTGTGAAAG CATTGATGGA ATGTGCTTTG GAAGTGAAAA AGGAATCAAC CCTCAAAAGT
    GTATTGAGTG CCTTATGGAA CTTGTCAGCA CACTGCACTG AGAATAAAGC TGACATATGT
    GCTGTGGATG GTGCGCTTGC ATTTTTGGTT GGCACTCTCA CTTATCGCAG CCAGACCAAT
    ACTTTAGCCA TTATTGAAAG TGGAGGTGGT ATATTACGGA ATGTATCCAG CTTGATAGCT
    ACAAATGAGG ACCACAGGCA AATCCTAAGA GAGAATAATT GCTTACAGAC CTTATTACAA
    CACTTGAAAT CTCACAGTTT GACAATAGTC AGCAATGCAT GCGGAACCTT GTGGAATCTC
    TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA TTGTGGGACA TGGGAGCAGT CAGCATGCTC
    AAGAACCTCA TTCATTCAAA GCACAAGATG ATTGCTATGG GAAGTGCTGC AGCTTTAAGG
    AATCTCATGG CAAATAGACC TGCAAAGTAT AAAGATGCCA ATATCATGTC TCCTGGTTCA
    AGTTTGCCTT CTCTACATGT CAGGAAACAA AAGGCCCTGG AAGCAGAATT AGATGCTCAG
    CATTTATCAG AAACTTTTGA CAATATTGAC AATTTAAGTC CCAAGGCATC TCATCGTAGC
    AAGCAGAGAC ACAAGCAAAA TCTCTATGGT GACTATGTTT TTGACACCAA TCGACATGAT
    GATAACAGGT CAGACAATTT TAATACTGGA AACATGACTG TCCTATCACC GTACTTAAAC
    ACTACAGTAT TGCCCAGCTC TTCTTCATCA AGGGGAAGTT TAGATAGCTC TCGTTCTGAG
    AAAGATAGAA GTCTGGAGAG AGAACGAGGT ATTAGCCTAG GCAACTATCA CCCAGCAACA
    GAAAACCCAG GAACCTCTTC AAAGCGAGGT TTGCAGATTT CTACCACTGC AGCCCAGATT
    GCCAAAGTCA TGGAAGAAGT ATCAGCTATT CATACCTCTC AGGAAGACAG AAGTTCTGGG
    TCTACCACAG AACTACACTG TGGGACAGAT GAGAGGAATG CACTAAGAAG AAGCTCTACC
    ACCCACACAC ATGCAAACAC GTACAACTTT ACCAAGTCAG AAAACTCAAA CAGAACATGT
    CCAATACCAT ATGCCAAAGT AGAATATAAG AGATCTTCAA ATGATAGTTT AAATAGTGTC
    AGCAGTAGTG ATGGTTATGG TAAAAGAGGT CAGATGAAAC CTTCAATTGA ATCCTATTCT
    GAAGATGATG AAAGTAAATT TTGCAGCTAT GGTCAGTATC CAGCTGACCT AGCCCATAAA
    ATACATAGTG CAAATCATAT GGATGATAAT GATGGAGAAC TAGATACACC AATAAATTAC
    AGTCTCAAAT ATTCCGATGA ACAGTTGAAC TCTGGGAGGC AGAGCCCTTC ACAGAATGAA
    AGATGGGCAA GACCCAAACA TATACTAGAA GATGAAATAA AACCGAATGA GCAGAGACAA
    TCAAGGAGTC AAAGCACAGC TTATCCCGTG TATCCTGAGA GCACTGATGA TAAACACCTC
    AAGTTCCAAC CACACTTTGG GCAGCAAGAA TGTGTTTCCC CATACAGGTC AAGAGCAGCC
    AATGGATCTG AAACAAATCG AGTAGGCTCT AATCATGGAA TTAGTCAAAA TGTGAACCAG
    TCTTTGTGTC AAGAAGATGA CTATGAAGAC GATAAACCAA CCAACTATAG CGAACGTTAC
    TCTGAGGAAG GGCAGCATGA GGAAGAAGAG AGGCCAACCA ATTATAGCAT AAAATACAGT
    GAAGAAAAAC ATCACGTGGA TCAGCCTATT GATTATAGTT TAAAATACAC CACAGACATT
    CCTTCTTCAC AGAAACCAGC ATTTTCATTC TCAAAGAATT CATCTGGACA GAGCACAAAA
    ACTGAACACA TCTCTTCAAG CAGCGAGAAT ACATCCACAA CTTCATCTAA TGCCAAGAGG
    CAGAATCAGC TGCATCCAAG CTCAGCACAG AGCAGAAGTG GTCAGACCCC AAAAGCCACC
    TCTTCCTCTT GCAAAGTCCC CTCTATCAAC CAAGAAACAA TACAGACTTA CTGTGTAGAA
    GATACCCCAA TATGTTTTTC AAGATGCAGT TCATTATCAT CTCTGTCATC TGCTGAAGAT
    GAAGTAGGGT GTGATCAGAC AACACAAGAA GCAGAGTCTG CTAACACTCT GCAAATAGCT
    GAAATCAAGG ACAACAGCGG ACCTAGGTCA AATGAAGATT CTGTGAGTAA AGTTCCAGCA
    GGGTCACAGC ACATTAGAAC CAAATCCAGC AGACTCCAGG CTTCTGGTCT GTCTTCAGAG
    TCAGCCAGGC ACAAAGCTGT TGAATTTTCT TCAGGGGCCA AATCTCCTTC CAAGAGCGGT
    GCTCAGACAC CCAAAAGTCC ACCAGAGCAC TACGTTCAGG AGACCCCACT CATGTTTAGC
    AGATGTACTT CAGTCAGTTC ACTCGACAGT TTTGAGAGTC GCTCGATTGC CAGCTCTGTT
    CAGAGTGAAC CCTGCAGTGG AATGGTAAGT GGCATCATAA GCCCCAGTGA CCTCCCAGAT
    AGCCCTGGAC AAACCATGCC ACCAAGCAGA AGCAAAACCC CTCCGCCACC GCCTCCGCCT
    CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA GTACCTAAAA ATAAAGCACC TAGTGCTGAA
    AAGAGAGAAA GTGGACCCAA GCAAGCTGCT GTAAATGCTG CAGTACAAAG GGTCCAGGTT
    CTTCCAGAGG CTGATACTCT GTTACATTTT GCCACAGAGA GTACTCCTGA TGGATTTTCT
    TGTTCATCTA GCCTGAGCGC TCTGAGCCTT GATGAGCCAT TTATTCAGAA AGATGTGGAA
    CTAAGAATAA TGCCTCCAGT TCAGGAAAAT GACAACGGGA ATGAAACAGA AAGTGAGCAG
    CCTGAAGAAT CAAATGAAAA CCAGGAAAAA GAGGCAGAAA AACCCACTGA TTCTGAAAAA
    GATCTTTTAG ATGAGTCAGA TGATGATGAT ATTGAAATAC TAGAAGAGTG TATAATTTCT
    GCCATGCCAA CAAAATCTTC ACGCAAAGCC AAAAAACCAG CCCAGACTAC TTCAAAATTA
    CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTCCCTGTAT ACAAACTTCT GCCATCGCAA
    AACAGGTTAC AGGCACAAAA GCATGTTAGT TTTACACCAG GAGATGATAT GCCACGGGTG
    TATTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCTA CATCTCTGAG TGATCTAACG
    ATAGAATCCC CTCCAAATGA GTTAGCTGCT GGAGAAGGGG TTAGAGCAGG GGCACAGTCA
    AGTGAGTTTG AAAAACGAGA TACCATTCCT ACTGAAGGCA GAAGTACAGA TGAGGCTCAA
    CGAGGGAAAG CCTCATCTGT CACTGTGCCT GAACTGGACG ACAGCAAAAC AGAAGAAGGT
    GATATTCTTG CAGAATGCAT TAATTCTGCC ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC
    CGTGTGAAAA AGATCATGGA CCAGGTCCAG CAAGCATCTA TGTCTTCATC TGGAACTAAC
    AAAAATCAAT TAGATGGTAA GACGAAGAAA CCTACTTCAC CAGTAAAACC TATACCACAA
    AATACTGAAT ACAGGACACG TGTAAGAAAA AATACAGACT CAAAAAATAA TCTAAATGCA
    GAAAGAAATT TCTCGGAAAA CAAAGACTCA AAGAAACAGC ACTTGAAAAA TAATTCCAAG
    GACTTCAATG ATAAACTGCC AAATAATGAA GACAGAGTCA GAGGAAGTTT TACTTTTGAT
    TCACCCCATC ATTACACGCC CATTGAAGGC ACTCCGTACT GTTTTTCACG AAATGACTCT
    CTGAGTTCTC TAGATTTTGA TGATGATGAT GTTGACCTTT CCAGGGAAAA GGCTGAATTA
    AGAAAGGGGA AGGAAAATAA AGAATCAGAA GCTAAAGTGA CCAACCACAC AGAACTAACC
    TCAAACCAAC AATCAGCTAG TAAGACACCA GCTGTTACAA AGCAGCCAAT AAATAGAGGT
    CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC ACTTTTCCCC AGTCTTCCAA AGATATACCA
    GACAGAGGGG CAGCAACAGA TGAGAAATTA CAGAATTTCG CTATTGAAAA TACTCCAGTT
    TGCTTTTCTC GAAATTCCTC TCTAAGCTCT CTTAGTGACA TTGATCAAGA AAACAACAAC
    AACAAGGAAA ATGAACCTGT CAAAGAGACA GAGCCCCCTG CCTCACAGGG AGAACCAGGT
    AAACCCCAGG CCTCAGGTTA TGCTCCTAAA TCGTTTCACG TGGAAGACAC CCCTGTTTGT
    TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT AGTATTGATT CTGAAGATGA CCTGCTGCAG
    GAATGTATAA GTTCTGCAAT GCCAAAAAAG AAAAAGCCTT CAAGGCTCAA GCCCGATAAT
    GAAAAGCATA GTCCCAGAAA TATGGGTGGC ATATTAGCAG AAGATTTGAC ACTCGATTTG
    AAAGATATAC AGAGACCAGA TTCAGAACAT GGTTTATCCC CAGATTCAGA AAATTTTGAT
    TGGAAAGCTA TTCAGGAAGG TGCAAATTCC ATAGTAAGTA GTTTACATCA AGCTGCTGCC
    GCTGCATGTT TATCTCGACA AGCTTCATCT GATTCAGATT CTATCCTTTC ATTGAAATCG
    GGTATCTCTC TGGGATCACC ATTTCACCTT ACACCTGATC AAGAAGAAAA ACCCTTTACG
    AGTAATAAAG GTCCACGAAT TCTAAAACCT GGGGAGAAAA GTACATTGGA AACTAAAAAA
    ATAGAATCTG AAAATAAAGG AATAAAAGGA GGCAAAAAGG TTTATAAAAG TTTGATTACT
    GGAAAAGTTC GATCTAATTC GGAAATTTCG AGCCAAATGA AACAACCCCT TCAAACAAAC
    ATGCCTTCAA TCTCTCGAGG TAGGACAATG ATTCATATTC CAGGAGTTCG GAATAGCTCT
    TCAAGTACAA GCCCAGTTTC TAAAAAAGGC CCGCCCCTCA AGACTCCAGC CTCCAAAAGC
    CCTAGTGAAG GTCAGCCGGC TACCACCTCT CCCAGAGGAA CCAAGCCATC AGTGAAGTCA
    GAATTAAGCC CTGTTACAAG GCAGGCATCC CAGACAGCAG GATCAAACAA AGGACCTTCT
    AGATCAGGAT CTAGAGATTC CACTCCTTCA AGACCTGCCC AGCAACCATT AAGTAGACCA
    ATGCAGTCTC CAGGGCGAAA CTCAATCTCT CCTGGTAGAA ATGGAATAAG TCCCCCTAAC
    AAATTATCTC AACTACCAAG GACGTCATCC CCTAGTACTG CTTCAACTAA GTCCTCAGGT
    TCTGGGAAAA TGTCTTACAC ATCTCCTGGC AGACAGATGA GCCAGCAGAA CCTCACCAAA
    CAAACAGGCT TATCCAAGAA TGGCAGTGGT ATCCCAAGAA GTGAATCTGC CTCCAAAGGG
    CTAAATCAAA TGAGTAATAG TAATGGATCC AATAAAAAAG TAGAACTTTC TAGAATGTCT
    TCAACAAAGT CAAGTGGAAG TGAATCCGAT AGGTCAGAGA GACCTGTATT AGTACGCCAA
    TCAACTTTCA TCAAAGAAGC TCCAAGCCCA ACCCTAAGGA GAAAACTGGA GGAATCTGCT
    TCATTTGAAT CTCTTTCTCC ATCTTCCAGA CCCGATTCTC CCACACGGTC CCAGGCTCAT
    ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT ATGTCTCTAT CTACACATTC GTCTCTTCAG
    TCTGGTGGAT GGCGAAAACT CCCACCTAAC CTCAGTCCCA CCATAGAGTA TAATGATGGA
    AGACCAGTAA AGCGCCATGA TATAGCACGC TCTCATTCCG AAAGTCCTTC CAGACTTCCC
    ATCAATAGGT CAGGGACCTG GAAACGTGAG CACAGCAAAC ACTCATCATC ACTTCCTCGA
    GTAAGCACTT GGAGAAGAAC TGGAAGTTCA TCCTCAATTC TTTCTGCTTC ATCAGAATCT
    AGTGAAAAGG CAAAAAGTGA GGACGAAAAA CAAGTGAACT CTATTTCAGG AAGCAAACAA
    ACTAAAGAAA ACCAGGTATC CACAAAAGGA ACATGGAGAA AAATAAAAGA AAGTGAAATT
    TCTCCCACAA ATAGTACTTC TCAGACCACT TCTTCAGGTG CTGCAAATGG TGCTGAATCA
    AAGACTCTGA TTTATCAAAT GGCACCTGCT GTTTCTAAAA CAGAGGATGT TTGGGTGAGA
    ATTGAGGATT GCCCCATTAA CAACCCTAGA TCTGGAAGAT CTCCAACAGG AAATACTCCC
    CCCGTGATTG ACACTGTTTC AGAAAAGGGA AACCCGAACC CTAAAGATTC AAAAGATAAT
    CAGGGAAAAC AAAATGTGAG CAATGGTAGT GCCCCTACAC GCACCATGGG TCTGGAAAAC
    CGCCTGAATT CCTTTATTCA GGTAGATCCT CCAGACCAAA AAGGAACTGA GACAAAACCG
    GGACACAGTA ATAACCCTGT CCCTGCATCC GAGACTAGTG AAAGTTCTAT AGCCGAGCGT
    ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG CACAGTTCAC CAAGTGGGAC TGTCGCCGCC
    AGGGTGAGTC CTTTTAATTA CAACCCAAGC CCAAGGAAGA GCAGCACAGA TGGCACTTCA
    GCCCGACCGT CTCAGATCCC AACGCCAGTG AGCAACAACA CAAAGAAACG CGACTCAAAA
    CCCGACAGCA CAGAACCCAG CGGGACTCAA AGTCCTAAAC GCCATTCTGG GTCTTACCTT
    GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328473.1
    CDS16..8370
    Translation

    Target ORF information:

    RefSeq Version XM_015472987.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTGCCATG TTCTTCTAGA TGATCCTGAG TTAGCTAGAG GACTAAGAAG ACATGAAATT 
    TATGTCAGAT CATTGCTTCT TGCTGACCTT GACAAAGAAG AAAAGGAAAA AGACTGGTAT
    TATGCTCAAC TTCAGAATCT CACTAAAAGA ATAGATAGCC TTCCTTTAAC TGAAAATTTT
    TCCTTACAAA CAGATATGAC CAGAAGGCAG TTGGAATATG AGGCAAGGCA AATCAGAGTT
    GCAATGGAAG AACAACTAGG TACTTGCCAG GATATGGAAA AACGAGCACA GCGAAGAATA
    ACCAGAATTC AACAAATAGA AAAGGACATA CTTCGTATAC GACAGCTTTT ACAGTCCCAA
    GCAACAGAAG CAGAGAGGTC ATCTCAGAGC AAGCATGAAG CGGGCTCACA TGAAGCTGAA
    CGGCAGAATG AAGGTCAAGG AGTGGCAGAA ATCAACATGG CAACCTCGGG TAGCGGTCAG
    GTTACTGCTT CTCTGGTCCC TCTGAAGGAT GTCGGTGCAG ACTTAATCAT ACTAAAAAGG
    CCCAGGAAAG ATTCTCAAGA CTCGGGTTCT GGCTTACAGG GTTCAACTAC ACGAATAGAT
    CATGAAACAG CCAGTGTTTT GAGTTCTAGT AGCACACATT CTGCTCCTCG AAGGCTGACG
    AGTCATCTGG GAACCAAGGT GGAAATGGTG TATTCATTGT TGTCAATGCT TGGTACTCAT
    GATAAGGATG ATATGTCGCG AACTTTGCTA GCTATGTCTA GCTCCCAAGA CAGCTGTATA
    TCCATGCGAC AGTCTGGATG TCTTCCTCTC CTCATCCAGC TTTTACATGG CAATGACAAA
    GACTCTGTGT TGTTGGGAAA TTCCCGGGGC AGTAAAGAGG CTCGGGCCAG GGCCAGTGCA
    GCACTCCACA ACATCATTCA CTCACAGCCT GATGACAAGA GAGGCAGGCG TGAAATCCGA
    GTCCTTCATC TTTTGGAACA GATACGAGCT TACTGTGAAA CCTGTTGGGA GTGGCAGGAA
    GCCCATGAAC AAGGGATGGA CCAGGACAAA AATCCAATGC CAGCTCCTGT TGAACATCAG
    ATCTGCCCTG CTGTCTGTGT TCTAATGAAG CTTTCGTTTG ATGAAGAGCA TAGACATGCC
    ATGAATGAGC TTGGTAGGAA GGCTACCCGG GGCATTTCAT CCCAGGAGCT AGGGCAGGGG
    CTTTCAGGGG GACTACAGGC CATTGCAGAA TTATTGCAAG TGGACTGTGA AATGTATGGG
    CTTACTAATG ACCACTACAG TATTACTTTA AGACGATATG CAGGAATGGC TTTGACAAAC
    TTGACTTTCG GAGATGTAGC CAACAAGGCT ACTCTCTGCT CTATGAAAGG CTGCATGAGA
    GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC GAGGACTTGC AGCAGGTTAT TGCGAGTGTT
    TTGAGGAATC TGTCTTGGAG AGCAGATGTA AATAGTAAAA AGACTTTGCG TGAAGTTGGA
    AGTGTGAAAG CATTGATGGA ATGTGCTTTG GAAGTGAAAA AGGAATCAAC CCTCAAAAGT
    GTATTGAGTG CCTTATGGAA CTTGTCAGCA CACTGCACTG AGAATAAAGC TGACATATGT
    GCTGTGGATG GTGCGCTTGC ATTTTTGGTT GGCACTCTCA CTTATCGCAG CCAGACCAAT
    ACTTTAGCCA TTATTGAAAG TGGAGGTGGT ATATTACGGA ATGTATCCAG CTTGATAGCT
    ACAAATGAGG ACCACAGGCA AATCCTAAGA GAGAATAATT GCTTACAGAC CTTATTACAA
    CACTTGAAAT CTCACAGTTT GACAATAGTC AGCAATGCAT GCGGAACCTT GTGGAATCTC
    TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA TTGTGGGACA TGGGAGCAGT CAGCATGCTC
    AAGAACCTCA TTCATTCAAA GCACAAGATG ATTGCTATGG GAAGTGCTGC AGCTTTAAGG
    AATCTCATGG CAAATAGACC TGCAAAGTAT AAAGATGCCA ATATCATGTC TCCTGGTTCA
    AGTTTGCCTT CTCTACATGT CAGGAAACAA AAGGCCCTGG AAGCAGAATT AGATGCTCAG
    CATTTATCAG AAACTTTTGA CAATATTGAC AATTTAAGTC CCAAGGCATC TCATCGTAGC
    AAGCAGAGAC ACAAGCAAAA TCTCTATGGT GACTATGTTT TTGACACCAA TCGACATGAT
    GATAACAGGT CAGACAATTT TAATACTGGA AACATGACTG TCCTATCACC GTACTTAAAC
    ACTACAGTAT TGCCCAGCTC TTCTTCATCA AGGGGAAGTT TAGATAGCTC TCGTTCTGAG
    AAAGATAGAA GTCTGGAGAG AGAACGAGGT ATTAGCCTAG GCAACTATCA CCCAGCAACA
    GAAAACCCAG GAACCTCTTC AAAGCGAGGT TTGCAGATTT CTACCACTGC AGCCCAGATT
    GCCAAAGTCA TGGAAGAAGT ATCAGCTATT CATACCTCTC AGGAAGACAG AAGTTCTGGG
    TCTACCACAG AACTACACTG TGGGACAGAT GAGAGGAATG CACTAAGAAG AAGCTCTACC
    ACCCACACAC ATGCAAACAC GTACAACTTT ACCAAGTCAG AAAACTCAAA CAGAACATGT
    CCAATACCAT ATGCCAAAGT AGAATATAAG AGATCTTCAA ATGATAGTTT AAATAGTGTC
    AGCAGTAGTG ATGGTTATGG TAAAAGAGGT CAGATGAAAC CTTCAATTGA ATCCTATTCT
    GAAGATGATG AAAGTAAATT TTGCAGCTAT GGTCAGTATC CAGCTGACCT AGCCCATAAA
    ATACATAGTG CAAATCATAT GGATGATAAT GATGGAGAAC TAGATACACC AATAAATTAC
    AGTCTCAAAT ATTCCGATGA ACAGTTGAAC TCTGGGAGGC AGAGCCCTTC ACAGAATGAA
    AGATGGGCAA GACCCAAACA TATACTAGAA GATGAAATAA AACCGAATGA GCAGAGACAA
    TCAAGGAGTC AAAGCACAGC TTATCCCGTG TATCCTGAGA GCACTGATGA TAAACACCTC
    AAGTTCCAAC CACACTTTGG GCAGCAAGAA TGTGTTTCCC CATACAGGTC AAGAGCAGCC
    AATGGATCTG AAACAAATCG AGTAGGCTCT AATCATGGAA TTAGTCAAAA TGTGAACCAG
    TCTTTGTGTC AAGAAGATGA CTATGAAGAC GATAAACCAA CCAACTATAG CGAACGTTAC
    TCTGAGGAAG GGCAGCATGA GGAAGAAGAG AGGCCAACCA ATTATAGCAT AAAATACAGT
    GAAGAAAAAC ATCACGTGGA TCAGCCTATT GATTATAGTT TAAAATACAC CACAGACATT
    CCTTCTTCAC AGAAACCAGC ATTTTCATTC TCAAAGAATT CATCTGGACA GAGCACAAAA
    ACTGAACACA TCTCTTCAAG CAGCGAGAAT ACATCCACAA CTTCATCTAA TGCCAAGAGG
    CAGAATCAGC TGCATCCAAG CTCAGCACAG AGCAGAAGTG GTCAGACCCC AAAAGCCACC
    TCTTCCTCTT GCAAAGTCCC CTCTATCAAC CAAGAAACAA TACAGACTTA CTGTGTAGAA
    GATACCCCAA TATGTTTTTC AAGATGCAGT TCATTATCAT CTCTGTCATC TGCTGAAGAT
    GAAGTAGGGT GTGATCAGAC AACACAAGAA GCAGAGTCTG CTAACACTCT GCAAATAGCT
    GAAATCAAGG ACAACAGCGG ACCTAGGTCA AATGAAGATT CTGTGAGTAA AGTTCCAGCA
    GGGTCACAGC ACATTAGAAC CAAATCCAGC AGACTCCAGG CTTCTGGTCT GTCTTCAGAG
    TCAGCCAGGC ACAAAGCTGT TGAATTTTCT TCAGGGGCCA AATCTCCTTC CAAGAGCGGT
    GCTCAGACAC CCAAAAGTCC ACCAGAGCAC TACGTTCAGG AGACCCCACT CATGTTTAGC
    AGATGTACTT CAGTCAGTTC ACTCGACAGT TTTGAGAGTC GCTCGATTGC CAGCTCTGTT
    CAGAGTGAAC CCTGCAGTGG AATGGTAAGT GGCATCATAA GCCCCAGTGA CCTCCCAGAT
    AGCCCTGGAC AAACCATGCC ACCAAGCAGA AGCAAAACCC CTCCGCCACC GCCTCCGCCT
    CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA GTACCTAAAA ATAAAGCACC TAGTGCTGAA
    AAGAGAGAAA GTGGACCCAA GCAAGCTGCT GTAAATGCTG CAGTACAAAG GGTCCAGGTT
    CTTCCAGAGG CTGATACTCT GTTACATTTT GCCACAGAGA GTACTCCTGA TGGATTTTCT
    TGTTCATCTA GCCTGAGCGC TCTGAGCCTT GATGAGCCAT TTATTCAGAA AGATGTGGAA
    CTAAGAATAA TGCCTCCAGT TCAGGAAAAT GACAACGGGA ATGAAACAGA AAGTGAGCAG
    CCTGAAGAAT CAAATGAAAA CCAGGAAAAA GAGGCAGAAA AACCCACTGA TTCTGAAAAA
    GATCTTTTAG ATGAGTCAGA TGATGATGAT ATTGAAATAC TAGAAGAGTG TATAATTTCT
    GCCATGCCAA CAAAATCTTC ACGCAAAGCC AAAAAACCAG CCCAGACTAC TTCAAAATTA
    CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTCCCTGTAT ACAAACTTCT GCCATCGCAA
    AACAGGTTAC AGGCACAAAA GCATGTTAGT TTTACACCAG GAGATGATAT GCCACGGGTG
    TATTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCTA CATCTCTGAG TGATCTAACG
    ATAGAATCCC CTCCAAATGA GTTAGCTGCT GGAGAAGGGG TTAGAGCAGG GGCACAGTCA
    AGTGAGTTTG AAAAACGAGA TACCATTCCT ACTGAAGGCA GAAGTACAGA TGAGGCTCAA
    CGAGGGAAAG CCTCATCTGT CACTGTGCCT GAACTGGACG ACAGCAAAAC AGAAGAAGGT
    GATATTCTTG CAGAATGCAT TAATTCTGCC ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC
    CGTGTGAAAA AGATCATGGA CCAGGTCCAG CAAGCATCTA TGTCTTCATC TGGAACTAAC
    AAAAATCAAT TAGATGGTAA GACGAAGAAA CCTACTTCAC CAGTAAAACC TATACCACAA
    AATACTGAAT ACAGGACACG TGTAAGAAAA AATACAGACT CAAAAAATAA TCTAAATGCA
    GAAAGAAATT TCTCGGAAAA CAAAGACTCA AAGAAACAGC ACTTGAAAAA TAATTCCAAG
    GACTTCAATG ATAAACTGCC AAATAATGAA GACAGAGTCA GAGGAAGTTT TACTTTTGAT
    TCACCCCATC ATTACACGCC CATTGAAGGC ACTCCGTACT GTTTTTCACG AAATGACTCT
    CTGAGTTCTC TAGATTTTGA TGATGATGAT GTTGACCTTT CCAGGGAAAA GGCTGAATTA
    AGAAAGGGGA AGGAAAATAA AGAATCAGAA GCTAAAGTGA CCAACCACAC AGAACTAACC
    TCAAACCAAC AATCAGCTAG TAAGACACCA GCTGTTACAA AGCAGCCAAT AAATAGAGGT
    CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC ACTTTTCCCC AGTCTTCCAA AGATATACCA
    GACAGAGGGG CAGCAACAGA TGAGAAATTA CAGAATTTCG CTATTGAAAA TACTCCAGTT
    TGCTTTTCTC GAAATTCCTC TCTAAGCTCT CTTAGTGACA TTGATCAAGA AAACAACAAC
    AACAAGGAAA ATGAACCTGT CAAAGAGACA GAGCCCCCTG CCTCACAGGG AGAACCAGGT
    AAACCCCAGG CCTCAGGTTA TGCTCCTAAA TCGTTTCACG TGGAAGACAC CCCTGTTTGT
    TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT AGTATTGATT CTGAAGATGA CCTGCTGCAG
    GAATGTATAA GTTCTGCAAT GCCAAAAAAG AAAAAGCCTT CAAGGCTCAA GCCCGATAAT
    GAAAAGCATA GTCCCAGAAA TATGGGTGGC ATATTAGCAG AAGATTTGAC ACTCGATTTG
    AAAGATATAC AGAGACCAGA TTCAGAACAT GGTTTATCCC CAGATTCAGA AAATTTTGAT
    TGGAAAGCTA TTCAGGAAGG TGCAAATTCC ATAGTAAGTA GTTTACATCA AGCTGCTGCC
    GCTGCATGTT TATCTCGACA AGCTTCATCT GATTCAGATT CTATCCTTTC ATTGAAATCG
    GGTATCTCTC TGGGATCACC ATTTCACCTT ACACCTGATC AAGAAGAAAA ACCCTTTACG
    AGTAATAAAG GTCCACGAAT TCTAAAACCT GGGGAGAAAA GTACATTGGA AACTAAAAAA
    ATAGAATCTG AAAATAAAGG AATAAAAGGA GGCAAAAAGG TTTATAAAAG TTTGATTACT
    GGAAAAGTTC GATCTAATTC GGAAATTTCG AGCCAAATGA AACAACCCCT TCAAACAAAC
    ATGCCTTCAA TCTCTCGAGG TAGGACAATG ATTCATATTC CAGGAGTTCG GAATAGCTCT
    TCAAGTACAA GCCCAGTTTC TAAAAAAGGC CCGCCCCTCA AGACTCCAGC CTCCAAAAGC
    CCTAGTGAAG GTCAGCCGGC TACCACCTCT CCCAGAGGAA CCAAGCCATC AGTGAAGTCA
    GAATTAAGCC CTGTTACAAG GCAGGCATCC CAGACAGCAG GATCAAACAA AGGACCTTCT
    AGATCAGGAT CTAGAGATTC CACTCCTTCA AGACCTGCCC AGCAACCATT AAGTAGACCA
    ATGCAGTCTC CAGGGCGAAA CTCAATCTCT CCTGGTAGAA ATGGAATAAG TCCCCCTAAC
    AAATTATCTC AACTACCAAG GACGTCATCC CCTAGTACTG CTTCAACTAA GTCCTCAGGT
    TCTGGGAAAA TGTCTTACAC ATCTCCTGGC AGACAGATGA GCCAGCAGAA CCTCACCAAA
    CAAACAGGCT TATCCAAGAA TGGCAGTGGT ATCCCAAGAA GTGAATCTGC CTCCAAAGGG
    CTAAATCAAA TGAGTAATAG TAATGGATCC AATAAAAAAG TAGAACTTTC TAGAATGTCT
    TCAACAAAGT CAAGTGGAAG TGAATCCGAT AGGTCAGAGA GACCTGTATT AGTACGCCAA
    TCAACTTTCA TCAAAGAAGC TCCAAGCCCA ACCCTAAGGA GAAAACTGGA GGAATCTGCT
    TCATTTGAAT CTCTTTCTCC ATCTTCCAGA CCCGATTCTC CCACACGGTC CCAGGCTCAT
    ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT ATGTCTCTAT CTACACATTC GTCTCTTCAG
    TCTGGTGGAT GGCGAAAACT CCCACCTAAC CTCAGTCCCA CCATAGAGTA TAATGATGGA
    AGACCAGTAA AGCGCCATGA TATAGCACGC TCTCATTCCG AAAGTCCTTC CAGACTTCCC
    ATCAATAGGT CAGGGACCTG GAAACGTGAG CACAGCAAAC ACTCATCATC ACTTCCTCGA
    GTAAGCACTT GGAGAAGAAC TGGAAGTTCA TCCTCAATTC TTTCTGCTTC ATCAGAATCT
    AGTGAAAAGG CAAAAAGTGA GGACGAAAAA CAAGTGAACT CTATTTCAGG AAGCAAACAA
    ACTAAAGAAA ACCAGGTATC CACAAAAGGA ACATGGAGAA AAATAAAAGA AAGTGAAATT
    TCTCCCACAA ATAGTACTTC TCAGACCACT TCTTCAGGTG CTGCAAATGG TGCTGAATCA
    AAGACTCTGA TTTATCAAAT GGCACCTGCT GTTTCTAAAA CAGAGGATGT TTGGGTGAGA
    ATTGAGGATT GCCCCATTAA CAACCCTAGA TCTGGAAGAT CTCCAACAGG AAATACTCCC
    CCCGTGATTG ACACTGTTTC AGAAAAGGGA AACCCGAACC CTAAAGATTC AAAAGATAAT
    CAGGGAAAAC AAAATGTGAG CAATGGTAGT GCCCCTACAC GCACCATGGG TCTGGAAAAC
    CGCCTGAATT CCTTTATTCA GGTAGATCCT CCAGACCAAA AAGGAACTGA GACAAAACCG
    GGACACAGTA ATAACCCTGT CCCTGCATCC GAGACTAGTG AAAGTTCTAT AGCCGAGCGT
    ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG CACAGTTCAC CAAGTGGGAC TGTCGCCGCC
    AGGGTGAGTC CTTTTAATTA CAACCCAAGC CCAAGGAAGA GCAGCACAGA TGGCACTTCA
    GCCCGACCGT CTCAGATCCC AACGCCAGTG AGCAACAACA CAAAGAAACG CGACTCAAAA
    CCCGACAGCA CAGAACCCAG CGGGACTCAA AGTCCTAAAC GCCATTCTGG GTCTTACCTT
    GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219619
    Clone ID Related Accession (Same CDS sequence) XM_024997642.1
    Accession Version XM_024997642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2581
    2641
    2701
    2761
    2821
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    3001
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    3121
    3181
    3241
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    4141
    4201
    4261
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    4381
    4441
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    4681
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    4921
    4981
    5041
    5101
    5161
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    5281
    5341
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6961
    7021
    7081
    7141
    7201
    7261
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    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGTAGG
    AAGGCTACCC GGGGCATTTC ATCCCAGGAG CTAGGGCAGG GGCTTTCAGG GGGACTACAG
    GCCATTGCAG AATTATTGCA AGTGGACTGT GAAATGTATG GGCTTACTAA TGACCACTAC
    AGTATTACTT TAAGACGATA TGCAGGAATG GCTTTGACAA ACTTGACTTT CGGAGATGTA
    GCCAACAAGG CTACTCTCTG CTCTATGAAA GGCTGCATGA GAGCGCTCGT GGCTCAGCTC
    CAGTCTGAAA GCGAGGACTT GCAGCAGGTT ATTGCGAGTG TTTTGAGGAA TCTGTCTTGG
    AGAGCAGATG TAAATAGTAA AAAGACTTTG CGTGAAGTTG GAAGTGTGAA AGCATTGATG
    GAATGTGCTT TGGAAGTGAA AAAGGAATCA ACCCTCAAAA GTGTATTGAG TGCCTTATGG
    AACTTGTCAG CACACTGCAC TGAGAATAAA GCTGACATAT GTGCTGTGGA TGGTGCGCTT
    GCATTTTTGG TTGGCACTCT CACTTATCGC AGCCAGACCA ATACTTTAGC CATTATTGAA
    AGTGGAGGTG GTATATTACG GAATGTATCC AGCTTGATAG CTACAAATGA GGACCACAGG
    CAAATCCTAA GAGAGAATAA TTGCTTACAG ACCTTATTAC AACACTTGAA ATCTCACAGT
    TTGACAATAG TCAGCAATGC ATGCGGAACC TTGTGGAATC TCTCAGCAAG AAACCCTAAA
    GACCAGGAAG CATTGTGGGA CATGGGAGCA GTCAGCATGC TCAAGAACCT CATTCATTCA
    AAGCACAAGA TGATTGCTAT GGGAAGTGCT GCAGCTTTAA GGAATCTCAT GGCAAATAGA
    CCTGCAAAGT ATAAAGATGC CAATATCATG TCTCCTGGTT CAAGTTTGCC TTCTCTACAT
    GTCAGGAAAC AAAAGGCCCT GGAAGCAGAA TTAGATGCTC AGCATTTATC AGAAACTTTT
    GACAATATTG ACAATTTAAG TCCCAAGGCA TCTCATCGTA GCAAGCAGAG ACACAAGCAA
    AATCTCTATG GTGACTATGT TTTTGACACC AATCGACATG ATGATAACAG GTCAGACAAT
    TTTAATACTG GAAACATGAC TGTCCTATCA CCGTACTTAA ACACTACAGT ATTGCCCAGC
    TCTTCTTCAT CAAGGGGAAG TTTAGATAGC TCTCGTTCTG AGAAAGATAG AAGTCTGGAG
    AGAGAACGAG GTATTAGCCT AGGCAACTAT CACCCAGCAA CAGAAAACCC AGGAACCTCT
    TCAAAGCGAG GTTTGCAGAT TTCTACCACT GCAGCCCAGA TTGCCAAAGT CATGGAAGAA
    GTATCAGCTA TTCATACCTC TCAGGAAGAC AGAAGTTCTG GGTCTACCAC AGAACTACAC
    TGTGGGACAG ATGAGAGGAA TGCACTAAGA AGAAGCTCTA CCACCCACAC ACATGCAAAC
    ACGTACAACT TTACCAAGTC AGAAAACTCA AACAGAACAT GTCCAATACC ATATGCCAAA
    GTAGAATATA AGAGATCTTC AAATGATAGT TTAAATAGTG TCAGCAGTAG TGATGGTTAT
    GGTAAAAGAG GTCAGATGAA ACCTTCAATT GAATCCTATT CTGAAGATGA TGAAAGTAAA
    TTTTGCAGCT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AAATACATAG TGCAAATCAT
    ATGGATGATA ATGATGGAGA ACTAGATACA CCAATAAATT ACAGTCTCAA ATATTCCGAT
    GAACAGTTGA ACTCTGGGAG GCAGAGCCCT TCACAGAATG AAAGATGGGC AAGACCCAAA
    CATATACTAG AAGATGAAAT AAAACCGAAT GAGCAGAGAC AATCAAGGAG TCAAAGCACA
    GCTTATCCCG TGTATCCTGA GAGCACTGAT GATAAACACC TCAAGTTCCA ACCACACTTT
    GGGCAGCAAG AATGTGTTTC CCCATACAGG TCAAGAGCAG CCAATGGATC TGAAACAAAT
    CGAGTAGGCT CTAATCATGG AATTAGTCAA AATGTGAACC AGTCTTTGTG TCAAGAAGAT
    GACTATGAAG ACGATAAACC AACCAACTAT AGCGAACGTT ACTCTGAGGA AGGGCAGCAT
    GAGGAAGAAG AGAGGCCAAC CAATTATAGC ATAAAATACA GTGAAGAAAA ACATCACGTG
    GATCAGCCTA TTGATTATAG TTTAAAATAC ACCACAGACA TTCCTTCTTC ACAGAAACCA
    GCATTTTCAT TCTCAAAGAA TTCATCTGGA CAGAGCACAA AAACTGAACA CATCTCTTCA
    AGCAGCGAGA ATACATCCAC AACTTCATCT AATGCCAAGA GGCAGAATCA GCTGCATCCA
    AGCTCAGCAC AGAGCAGAAG TGGTCAGACC CCAAAAGCCA CCTCTTCCTC TTGCAAAGTC
    CCCTCTATCA ACCAAGAAAC AATACAGACT TACTGTGTAG AAGATACCCC AATATGTTTT
    TCAAGATGCA GTTCATTATC ATCTCTGTCA TCTGCTGAAG ATGAAGTAGG GTGTGATCAG
    ACAACACAAG AAGCAGAGTC TGCTAACACT CTGCAAATAG CTGAAATCAA GGACAACAGC
    GGACCTAGGT CAAATGAAGA TTCTGTGAGT AAAGTTCCAG CAGGGTCACA GCACATTAGA
    ACCAAATCCA GCAGACTCCA GGCTTCTGGT CTGTCTTCAG AGTCAGCCAG GCACAAAGCT
    GTTGAATTTT CTTCAGGGGC CAAATCTCCT TCCAAGAGCG GTGCTCAGAC ACCCAAAAGT
    CCACCAGAGC ACTACGTTCA GGAGACCCCA CTCATGTTTA GCAGATGTAC TTCAGTCAGT
    TCACTCGACA GTTTTGAGAG TCGCTCGATT GCCAGCTCTG TTCAGAGTGA ACCCTGCAGT
    GGAATGGTAA GTGGCATCAT AAGCCCCAGT GACCTCCCAG ATAGCCCTGG ACAAACCATG
    CCACCAAGCA GAAGCAAAAC CCCTCCGCCA CCGCCTCCGC CTCCTCCTCA GACAGTTCAA
    ACGAAGCAGG AAGTACCTAA AAATAAAGCA CCTAGTGCTG AAAAGAGAGA AAGTGGACCC
    AAGCAAGCTG CTGTAAATGC TGCAGTACAA AGGGTCCAGG TTCTTCCAGA GGCTGATACT
    CTGTTACATT TTGCCACAGA GAGTACTCCT GATGGATTTT CTTGTTCATC TAGCCTGAGC
    GCTCTGAGCC TTGATGAGCC ATTTATTCAG AAAGATGTGG AACTAAGAAT AATGCCTCCA
    GTTCAGGAAA ATGACAACGG GAATGAAACA GAAAGTGAGC AGCCTGAAGA ATCAAATGAA
    AACCAGGAAA AAGAGGCAGA AAAACCCACT GATTCTGAAA AAGATCTTTT AGATGAGTCA
    GATGATGATG ATATTGAAAT ACTAGAAGAG TGTATAATTT CTGCCATGCC AACAAAATCT
    TCACGCAAAG CCAAAAAACC AGCCCAGACT ACTTCAAAAT TACCTCCACC TGTGGCAAGG
    AAACCAAGTC AGCTCCCTGT ATACAAACTT CTGCCATCGC AAAACAGGTT ACAGGCACAA
    AAGCATGTTA GTTTTACACC AGGAGATGAT ATGCCACGGG TGTATTGTGT AGAAGGGACA
    CCTATAAACT TTTCCACAGC TACATCTCTG AGTGATCTAA CGATAGAATC CCCTCCAAAT
    GAGTTAGCTG CTGGAGAAGG GGTTAGAGCA GGGGCACAGT CAAGTGAGTT TGAAAAACGA
    GATACCATTC CTACTGAAGG CAGAAGTACA GATGAGGCTC AACGAGGGAA AGCCTCATCT
    GTCACTGTGC CTGAACTGGA CGACAGCAAA ACAGAAGAAG GTGATATTCT TGCAGAATGC
    ATTAATTCTG CCATGCCCAA AGGAAAAAGT CACAAGCCTT TCCGTGTGAA AAAGATCATG
    GACCAGGTCC AGCAAGCATC TATGTCTTCA TCTGGAACTA ACAAAAATCA ATTAGATGGT
    AAGACGAAGA AACCTACTTC ACCAGTAAAA CCTATACCAC AAAATACTGA ATACAGGACA
    CGTGTAAGAA AAAATACAGA CTCAAAAAAT AATCTAAATG CAGAAAGAAA TTTCTCGGAA
    AACAAAGACT CAAAGAAACA GCACTTGAAA AATAATTCCA AGGACTTCAA TGATAAACTG
    CCAAATAATG AAGACAGAGT CAGAGGAAGT TTTACTTTTG ATTCACCCCA TCATTACACG
    CCCATTGAAG GCACTCCGTA CTGTTTTTCA CGAAATGACT CTCTGAGTTC TCTAGATTTT
    GATGATGATG ATGTTGACCT TTCCAGGGAA AAGGCTGAAT TAAGAAAGGG GAAGGAAAAT
    AAAGAATCAG AAGCTAAAGT GACCAACCAC ACAGAACTAA CCTCAAACCA ACAATCAGCT
    AGTAAGACAC CAGCTGTTAC AAAGCAGCCA ATAAATAGAG GTCAGTCTAA ACCCGTGCTG
    CAGAAGCAGT CCACTTTTCC CCAGTCTTCC AAAGATATAC CAGACAGAGG GGCAGCAACA
    GATGAGAAAT TACAGAATTT CGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC
    TCTCTAAGCT CTCTTAGTGA CATTGATCAA GAAAACAACA ACAACAAGGA AAATGAACCT
    GTCAAAGAGA CAGAGCCCCC TGCCTCACAG GGAGAACCAG GTAAACCCCA GGCCTCAGGT
    TATGCTCCTA AATCGTTTCA CGTGGAAGAC ACCCCTGTTT GTTTCTCAAG AAACAGTTCT
    CTCAGTTCTC TTAGTATTGA TTCTGAAGAT GACCTGCTGC AGGAATGTAT AAGTTCTGCA
    ATGCCAAAAA AGAAAAAGCC TTCAAGGCTC AAGCCCGATA ATGAAAAGCA TAGTCCCAGA
    AATATGGGTG GCATATTAGC AGAAGATTTG ACACTCGATT TGAAAGATAT ACAGAGACCA
    GATTCAGAAC ATGGTTTATC CCCAGATTCA GAAAATTTTG ATTGGAAAGC TATTCAGGAA
    GGTGCAAATT CCATAGTAAG TAGTTTACAT CAAGCTGCTG CCGCTGCATG TTTATCTCGA
    CAAGCTTCAT CTGATTCAGA TTCTATCCTT TCATTGAAAT CGGGTATCTC TCTGGGATCA
    CCATTTCACC TTACACCTGA TCAAGAAGAA AAACCCTTTA CGAGTAATAA AGGTCCACGA
    ATTCTAAAAC CTGGGGAGAA AAGTACATTG GAAACTAAAA AAATAGAATC TGAAAATAAA
    GGAATAAAAG GAGGCAAAAA GGTTTATAAA AGTTTGATTA CTGGAAAAGT TCGATCTAAT
    TCGGAAATTT CGAGCCAAAT GAAACAACCC CTTCAAACAA ACATGCCTTC AATCTCTCGA
    GGTAGGACAA TGATTCATAT TCCAGGAGTT CGGAATAGCT CTTCAAGTAC AAGCCCAGTT
    TCTAAAAAAG GCCCGCCCCT CAAGACTCCA GCCTCCAAAA GCCCTAGTGA AGGTCAGCCG
    GCTACCACCT CTCCCAGAGG AACCAAGCCA TCAGTGAAGT CAGAATTAAG CCCTGTTACA
    AGGCAGGCAT CCCAGACAGC AGGATCAAAC AAAGGACCTT CTAGATCAGG ATCTAGAGAT
    TCCACTCCTT CAAGACCTGC CCAGCAACCA TTAAGTAGAC CAATGCAGTC TCCAGGGCGA
    AACTCAATCT CTCCTGGTAG AAATGGAATA AGTCCCCCTA ACAAATTATC TCAACTACCA
    AGGACGTCAT CCCCTAGTAC TGCTTCAACT AAGTCCTCAG GTTCTGGGAA AATGTCTTAC
    ACATCTCCTG GCAGACAGAT GAGCCAGCAG AACCTCACCA AACAAACAGG CTTATCCAAG
    AATGGCAGTG GTATCCCAAG AAGTGAATCT GCCTCCAAAG GGCTAAATCA AATGAGTAAT
    AGTAATGGAT CCAATAAAAA AGTAGAACTT TCTAGAATGT CTTCAACAAA GTCAAGTGGA
    AGTGAATCCG ATAGGTCAGA GAGACCTGTA TTAGTACGCC AATCAACTTT CATCAAAGAA
    GCTCCAAGCC CAACCCTAAG GAGAAAACTG GAGGAATCTG CTTCATTTGA ATCTCTTTCT
    CCATCTTCCA GACCCGATTC TCCCACACGG TCCCAGGCTC ATACTCCAGT TTTAAGTCCT
    TCCCTTCCTG ATATGTCTCT ATCTACACAT TCGTCTCTTC AGTCTGGTGG ATGGCGAAAA
    CTCCCACCTA ACCTCAGTCC CACCATAGAG TATAATGATG GAAGACCAGT AAAGCGCCAT
    GATATAGCAC GCTCTCATTC CGAAAGTCCT TCCAGACTTC CCATCAATAG GTCAGGGACC
    TGGAAACGTG AGCACAGCAA ACACTCATCA TCACTTCCTC GAGTAAGCAC TTGGAGAAGA
    ACTGGAAGTT CATCCTCAAT TCTTTCTGCT TCATCAGAAT CTAGTGAAAA GGCAAAAAGT
    GAGGACGAAA AACAAGTGAA CTCTATTTCA GGAAGCAAAC AAACTAAAGA AAACCAGGTA
    TCCACAAAAG GAACATGGAG AAAAATAAAA GAAAGTGAAA TTTCTCCCAC AAATAGTACT
    TCTCAGACCA CTTCTTCAGG TGCTGCAAAT GGTGCTGAAT CAAAGACTCT GATTTATCAA
    ATGGCACCTG CTGTTTCTAA AACAGAGGAT GTTTGGGTGA GAATTGAGGA TTGCCCCATT
    AACAACCCTA GATCTGGAAG ATCTCCAACA GGAAATACTC CCCCCGTGAT TGACACTGTT
    TCAGAAAAGG GAAACCCGAA CCCTAAAGAT TCAAAAGATA ATCAGGGAAA ACAAAATGTG
    AGCAATGGTA GTGCCCCTAC ACGCACCATG GGTCTGGAAA ACCGCCTGAA TTCCTTTATT
    CAGGTAGATC CTCCAGACCA AAAAGGAACT GAGACAAAAC CGGGACACAG TAATAACCCT
    GTCCCTGCAT CCGAGACTAG TGAAAGTTCT ATAGCCGAGC GTACCCCGTT TAGTTCTAGC
    AGCTCAAGCA AGCACAGTTC ACCAAGTGGG ACTGTCGCCG CCAGGGTGAG TCCTTTTAAT
    TACAACCCAA GCCCAAGGAA GAGCAGCACA GATGGCACTT CAGCCCGACC GTCTCAGATC
    CCAACGCCAG TGAGCAACAA CACAAAGAAA CGCGACTCAA AACCCGACAG CACAGAACCC
    AGCGGGACTC AAAGTCCTAA ACGCCATTCT GGGTCTTACC TTGTGACATC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853410.1
    CDS132..8468
    Translation

    Target ORF information:

    RefSeq Version XM_024997642.1
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGTAGG
    AAGGCTACCC GGGGCATTTC ATCCCAGGAG CTAGGGCAGG GGCTTTCAGG GGGACTACAG
    GCCATTGCAG AATTATTGCA AGTGGACTGT GAAATGTATG GGCTTACTAA TGACCACTAC
    AGTATTACTT TAAGACGATA TGCAGGAATG GCTTTGACAA ACTTGACTTT CGGAGATGTA
    GCCAACAAGG CTACTCTCTG CTCTATGAAA GGCTGCATGA GAGCGCTCGT GGCTCAGCTC
    CAGTCTGAAA GCGAGGACTT GCAGCAGGTT ATTGCGAGTG TTTTGAGGAA TCTGTCTTGG
    AGAGCAGATG TAAATAGTAA AAAGACTTTG CGTGAAGTTG GAAGTGTGAA AGCATTGATG
    GAATGTGCTT TGGAAGTGAA AAAGGAATCA ACCCTCAAAA GTGTATTGAG TGCCTTATGG
    AACTTGTCAG CACACTGCAC TGAGAATAAA GCTGACATAT GTGCTGTGGA TGGTGCGCTT
    GCATTTTTGG TTGGCACTCT CACTTATCGC AGCCAGACCA ATACTTTAGC CATTATTGAA
    AGTGGAGGTG GTATATTACG GAATGTATCC AGCTTGATAG CTACAAATGA GGACCACAGG
    CAAATCCTAA GAGAGAATAA TTGCTTACAG ACCTTATTAC AACACTTGAA ATCTCACAGT
    TTGACAATAG TCAGCAATGC ATGCGGAACC TTGTGGAATC TCTCAGCAAG AAACCCTAAA
    GACCAGGAAG CATTGTGGGA CATGGGAGCA GTCAGCATGC TCAAGAACCT CATTCATTCA
    AAGCACAAGA TGATTGCTAT GGGAAGTGCT GCAGCTTTAA GGAATCTCAT GGCAAATAGA
    CCTGCAAAGT ATAAAGATGC CAATATCATG TCTCCTGGTT CAAGTTTGCC TTCTCTACAT
    GTCAGGAAAC AAAAGGCCCT GGAAGCAGAA TTAGATGCTC AGCATTTATC AGAAACTTTT
    GACAATATTG ACAATTTAAG TCCCAAGGCA TCTCATCGTA GCAAGCAGAG ACACAAGCAA
    AATCTCTATG GTGACTATGT TTTTGACACC AATCGACATG ATGATAACAG GTCAGACAAT
    TTTAATACTG GAAACATGAC TGTCCTATCA CCGTACTTAA ACACTACAGT ATTGCCCAGC
    TCTTCTTCAT CAAGGGGAAG TTTAGATAGC TCTCGTTCTG AGAAAGATAG AAGTCTGGAG
    AGAGAACGAG GTATTAGCCT AGGCAACTAT CACCCAGCAA CAGAAAACCC AGGAACCTCT
    TCAAAGCGAG GTTTGCAGAT TTCTACCACT GCAGCCCAGA TTGCCAAAGT CATGGAAGAA
    GTATCAGCTA TTCATACCTC TCAGGAAGAC AGAAGTTCTG GGTCTACCAC AGAACTACAC
    TGTGGGACAG ATGAGAGGAA TGCACTAAGA AGAAGCTCTA CCACCCACAC ACATGCAAAC
    ACGTACAACT TTACCAAGTC AGAAAACTCA AACAGAACAT GTCCAATACC ATATGCCAAA
    GTAGAATATA AGAGATCTTC AAATGATAGT TTAAATAGTG TCAGCAGTAG TGATGGTTAT
    GGTAAAAGAG GTCAGATGAA ACCTTCAATT GAATCCTATT CTGAAGATGA TGAAAGTAAA
    TTTTGCAGCT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AAATACATAG TGCAAATCAT
    ATGGATGATA ATGATGGAGA ACTAGATACA CCAATAAATT ACAGTCTCAA ATATTCCGAT
    GAACAGTTGA ACTCTGGGAG GCAGAGCCCT TCACAGAATG AAAGATGGGC AAGACCCAAA
    CATATACTAG AAGATGAAAT AAAACCGAAT GAGCAGAGAC AATCAAGGAG TCAAAGCACA
    GCTTATCCCG TGTATCCTGA GAGCACTGAT GATAAACACC TCAAGTTCCA ACCACACTTT
    GGGCAGCAAG AATGTGTTTC CCCATACAGG TCAAGAGCAG CCAATGGATC TGAAACAAAT
    CGAGTAGGCT CTAATCATGG AATTAGTCAA AATGTGAACC AGTCTTTGTG TCAAGAAGAT
    GACTATGAAG ACGATAAACC AACCAACTAT AGCGAACGTT ACTCTGAGGA AGGGCAGCAT
    GAGGAAGAAG AGAGGCCAAC CAATTATAGC ATAAAATACA GTGAAGAAAA ACATCACGTG
    GATCAGCCTA TTGATTATAG TTTAAAATAC ACCACAGACA TTCCTTCTTC ACAGAAACCA
    GCATTTTCAT TCTCAAAGAA TTCATCTGGA CAGAGCACAA AAACTGAACA CATCTCTTCA
    AGCAGCGAGA ATACATCCAC AACTTCATCT AATGCCAAGA GGCAGAATCA GCTGCATCCA
    AGCTCAGCAC AGAGCAGAAG TGGTCAGACC CCAAAAGCCA CCTCTTCCTC TTGCAAAGTC
    CCCTCTATCA ACCAAGAAAC AATACAGACT TACTGTGTAG AAGATACCCC AATATGTTTT
    TCAAGATGCA GTTCATTATC ATCTCTGTCA TCTGCTGAAG ATGAAGTAGG GTGTGATCAG
    ACAACACAAG AAGCAGAGTC TGCTAACACT CTGCAAATAG CTGAAATCAA GGACAACAGC
    GGACCTAGGT CAAATGAAGA TTCTGTGAGT AAAGTTCCAG CAGGGTCACA GCACATTAGA
    ACCAAATCCA GCAGACTCCA GGCTTCTGGT CTGTCTTCAG AGTCAGCCAG GCACAAAGCT
    GTTGAATTTT CTTCAGGGGC CAAATCTCCT TCCAAGAGCG GTGCTCAGAC ACCCAAAAGT
    CCACCAGAGC ACTACGTTCA GGAGACCCCA CTCATGTTTA GCAGATGTAC TTCAGTCAGT
    TCACTCGACA GTTTTGAGAG TCGCTCGATT GCCAGCTCTG TTCAGAGTGA ACCCTGCAGT
    GGAATGGTAA GTGGCATCAT AAGCCCCAGT GACCTCCCAG ATAGCCCTGG ACAAACCATG
    CCACCAAGCA GAAGCAAAAC CCCTCCGCCA CCGCCTCCGC CTCCTCCTCA GACAGTTCAA
    ACGAAGCAGG AAGTACCTAA AAATAAAGCA CCTAGTGCTG AAAAGAGAGA AAGTGGACCC
    AAGCAAGCTG CTGTAAATGC TGCAGTACAA AGGGTCCAGG TTCTTCCAGA GGCTGATACT
    CTGTTACATT TTGCCACAGA GAGTACTCCT GATGGATTTT CTTGTTCATC TAGCCTGAGC
    GCTCTGAGCC TTGATGAGCC ATTTATTCAG AAAGATGTGG AACTAAGAAT AATGCCTCCA
    GTTCAGGAAA ATGACAACGG GAATGAAACA GAAAGTGAGC AGCCTGAAGA ATCAAATGAA
    AACCAGGAAA AAGAGGCAGA AAAACCCACT GATTCTGAAA AAGATCTTTT AGATGAGTCA
    GATGATGATG ATATTGAAAT ACTAGAAGAG TGTATAATTT CTGCCATGCC AACAAAATCT
    TCACGCAAAG CCAAAAAACC AGCCCAGACT ACTTCAAAAT TACCTCCACC TGTGGCAAGG
    AAACCAAGTC AGCTCCCTGT ATACAAACTT CTGCCATCGC AAAACAGGTT ACAGGCACAA
    AAGCATGTTA GTTTTACACC AGGAGATGAT ATGCCACGGG TGTATTGTGT AGAAGGGACA
    CCTATAAACT TTTCCACAGC TACATCTCTG AGTGATCTAA CGATAGAATC CCCTCCAAAT
    GAGTTAGCTG CTGGAGAAGG GGTTAGAGCA GGGGCACAGT CAAGTGAGTT TGAAAAACGA
    GATACCATTC CTACTGAAGG CAGAAGTACA GATGAGGCTC AACGAGGGAA AGCCTCATCT
    GTCACTGTGC CTGAACTGGA CGACAGCAAA ACAGAAGAAG GTGATATTCT TGCAGAATGC
    ATTAATTCTG CCATGCCCAA AGGAAAAAGT CACAAGCCTT TCCGTGTGAA AAAGATCATG
    GACCAGGTCC AGCAAGCATC TATGTCTTCA TCTGGAACTA ACAAAAATCA ATTAGATGGT
    AAGACGAAGA AACCTACTTC ACCAGTAAAA CCTATACCAC AAAATACTGA ATACAGGACA
    CGTGTAAGAA AAAATACAGA CTCAAAAAAT AATCTAAATG CAGAAAGAAA TTTCTCGGAA
    AACAAAGACT CAAAGAAACA GCACTTGAAA AATAATTCCA AGGACTTCAA TGATAAACTG
    CCAAATAATG AAGACAGAGT CAGAGGAAGT TTTACTTTTG ATTCACCCCA TCATTACACG
    CCCATTGAAG GCACTCCGTA CTGTTTTTCA CGAAATGACT CTCTGAGTTC TCTAGATTTT
    GATGATGATG ATGTTGACCT TTCCAGGGAA AAGGCTGAAT TAAGAAAGGG GAAGGAAAAT
    AAAGAATCAG AAGCTAAAGT GACCAACCAC ACAGAACTAA CCTCAAACCA ACAATCAGCT
    AGTAAGACAC CAGCTGTTAC AAAGCAGCCA ATAAATAGAG GTCAGTCTAA ACCCGTGCTG
    CAGAAGCAGT CCACTTTTCC CCAGTCTTCC AAAGATATAC CAGACAGAGG GGCAGCAACA
    GATGAGAAAT TACAGAATTT CGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC
    TCTCTAAGCT CTCTTAGTGA CATTGATCAA GAAAACAACA ACAACAAGGA AAATGAACCT
    GTCAAAGAGA CAGAGCCCCC TGCCTCACAG GGAGAACCAG GTAAACCCCA GGCCTCAGGT
    TATGCTCCTA AATCGTTTCA CGTGGAAGAC ACCCCTGTTT GTTTCTCAAG AAACAGTTCT
    CTCAGTTCTC TTAGTATTGA TTCTGAAGAT GACCTGCTGC AGGAATGTAT AAGTTCTGCA
    ATGCCAAAAA AGAAAAAGCC TTCAAGGCTC AAGCCCGATA ATGAAAAGCA TAGTCCCAGA
    AATATGGGTG GCATATTAGC AGAAGATTTG ACACTCGATT TGAAAGATAT ACAGAGACCA
    GATTCAGAAC ATGGTTTATC CCCAGATTCA GAAAATTTTG ATTGGAAAGC TATTCAGGAA
    GGTGCAAATT CCATAGTAAG TAGTTTACAT CAAGCTGCTG CCGCTGCATG TTTATCTCGA
    CAAGCTTCAT CTGATTCAGA TTCTATCCTT TCATTGAAAT CGGGTATCTC TCTGGGATCA
    CCATTTCACC TTACACCTGA TCAAGAAGAA AAACCCTTTA CGAGTAATAA AGGTCCACGA
    ATTCTAAAAC CTGGGGAGAA AAGTACATTG GAAACTAAAA AAATAGAATC TGAAAATAAA
    GGAATAAAAG GAGGCAAAAA GGTTTATAAA AGTTTGATTA CTGGAAAAGT TCGATCTAAT
    TCGGAAATTT CGAGCCAAAT GAAACAACCC CTTCAAACAA ACATGCCTTC AATCTCTCGA
    GGTAGGACAA TGATTCATAT TCCAGGAGTT CGGAATAGCT CTTCAAGTAC AAGCCCAGTT
    TCTAAAAAAG GCCCGCCCCT CAAGACTCCA GCCTCCAAAA GCCCTAGTGA AGGTCAGCCG
    GCTACCACCT CTCCCAGAGG AACCAAGCCA TCAGTGAAGT CAGAATTAAG CCCTGTTACA
    AGGCAGGCAT CCCAGACAGC AGGATCAAAC AAAGGACCTT CTAGATCAGG ATCTAGAGAT
    TCCACTCCTT CAAGACCTGC CCAGCAACCA TTAAGTAGAC CAATGCAGTC TCCAGGGCGA
    AACTCAATCT CTCCTGGTAG AAATGGAATA AGTCCCCCTA ACAAATTATC TCAACTACCA
    AGGACGTCAT CCCCTAGTAC TGCTTCAACT AAGTCCTCAG GTTCTGGGAA AATGTCTTAC
    ACATCTCCTG GCAGACAGAT GAGCCAGCAG AACCTCACCA AACAAACAGG CTTATCCAAG
    AATGGCAGTG GTATCCCAAG AAGTGAATCT GCCTCCAAAG GGCTAAATCA AATGAGTAAT
    AGTAATGGAT CCAATAAAAA AGTAGAACTT TCTAGAATGT CTTCAACAAA GTCAAGTGGA
    AGTGAATCCG ATAGGTCAGA GAGACCTGTA TTAGTACGCC AATCAACTTT CATCAAAGAA
    GCTCCAAGCC CAACCCTAAG GAGAAAACTG GAGGAATCTG CTTCATTTGA ATCTCTTTCT
    CCATCTTCCA GACCCGATTC TCCCACACGG TCCCAGGCTC ATACTCCAGT TTTAAGTCCT
    TCCCTTCCTG ATATGTCTCT ATCTACACAT TCGTCTCTTC AGTCTGGTGG ATGGCGAAAA
    CTCCCACCTA ACCTCAGTCC CACCATAGAG TATAATGATG GAAGACCAGT AAAGCGCCAT
    GATATAGCAC GCTCTCATTC CGAAAGTCCT TCCAGACTTC CCATCAATAG GTCAGGGACC
    TGGAAACGTG AGCACAGCAA ACACTCATCA TCACTTCCTC GAGTAAGCAC TTGGAGAAGA
    ACTGGAAGTT CATCCTCAAT TCTTTCTGCT TCATCAGAAT CTAGTGAAAA GGCAAAAAGT
    GAGGACGAAA AACAAGTGAA CTCTATTTCA GGAAGCAAAC AAACTAAAGA AAACCAGGTA
    TCCACAAAAG GAACATGGAG AAAAATAAAA GAAAGTGAAA TTTCTCCCAC AAATAGTACT
    TCTCAGACCA CTTCTTCAGG TGCTGCAAAT GGTGCTGAAT CAAAGACTCT GATTTATCAA
    ATGGCACCTG CTGTTTCTAA AACAGAGGAT GTTTGGGTGA GAATTGAGGA TTGCCCCATT
    AACAACCCTA GATCTGGAAG ATCTCCAACA GGAAATACTC CCCCCGTGAT TGACACTGTT
    TCAGAAAAGG GAAACCCGAA CCCTAAAGAT TCAAAAGATA ATCAGGGAAA ACAAAATGTG
    AGCAATGGTA GTGCCCCTAC ACGCACCATG GGTCTGGAAA ACCGCCTGAA TTCCTTTATT
    CAGGTAGATC CTCCAGACCA AAAAGGAACT GAGACAAAAC CGGGACACAG TAATAACCCT
    GTCCCTGCAT CCGAGACTAG TGAAAGTTCT ATAGCCGAGC GTACCCCGTT TAGTTCTAGC
    AGCTCAAGCA AGCACAGTTC ACCAAGTGGG ACTGTCGCCG CCAGGGTGAG TCCTTTTAAT
    TACAACCCAA GCCCAAGGAA GAGCAGCACA GATGGCACTT CAGCCCGACC GTCTCAGATC
    CCAACGCCAG TGAGCAACAA CACAAAGAAA CGCGACTCAA AACCCGACAG CACAGAACCC
    AGCGGGACTC AAAGTCCTAA ACGCCATTCT GGGTCTTACC TTGTGACATC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219620
    Clone ID Related Accession (Same CDS sequence) XM_005211202.4
    Accession Version XM_005211202.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211202.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGGGGA
    CTACAGGCCA TTGCAGAATT ATTGCAAGTG GACTGTGAAA TGTATGGGCT TACTAATGAC
    CACTACAGTA TTACTTTAAG ACGATATGCA GGAATGGCTT TGACAAACTT GACTTTCGGA
    GATGTAGCCA ACAAGGCTAC TCTCTGCTCT ATGAAAGGCT GCATGAGAGC GCTCGTGGCT
    CAGCTCCAGT CTGAAAGCGA GGACTTGCAG CAGGTTATTG CGAGTGTTTT GAGGAATCTG
    TCTTGGAGAG CAGATGTAAA TAGTAAAAAG ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA
    TTGATGGAAT GTGCTTTGGA AGTGAAAAAG GAATCAACCC TCAAAAGTGT ATTGAGTGCC
    TTATGGAACT TGTCAGCACA CTGCACTGAG AATAAAGCTG ACATATGTGC TGTGGATGGT
    GCGCTTGCAT TTTTGGTTGG CACTCTCACT TATCGCAGCC AGACCAATAC TTTAGCCATT
    ATTGAAAGTG GAGGTGGTAT ATTACGGAAT GTATCCAGCT TGATAGCTAC AAATGAGGAC
    CACAGGCAAA TCCTAAGAGA GAATAATTGC TTACAGACCT TATTACAACA CTTGAAATCT
    CACAGTTTGA CAATAGTCAG CAATGCATGC GGAACCTTGT GGAATCTCTC AGCAAGAAAC
    CCTAAAGACC AGGAAGCATT GTGGGACATG GGAGCAGTCA GCATGCTCAA GAACCTCATT
    CATTCAAAGC ACAAGATGAT TGCTATGGGA AGTGCTGCAG CTTTAAGGAA TCTCATGGCA
    AATAGACCTG CAAAGTATAA AGATGCCAAT ATCATGTCTC CTGGTTCAAG TTTGCCTTCT
    CTACATGTCA GGAAACAAAA GGCCCTGGAA GCAGAATTAG ATGCTCAGCA TTTATCAGAA
    ACTTTTGACA ATATTGACAA TTTAAGTCCC AAGGCATCTC ATCGTAGCAA GCAGAGACAC
    AAGCAAAATC TCTATGGTGA CTATGTTTTT GACACCAATC GACATGATGA TAACAGGTCA
    GACAATTTTA ATACTGGAAA CATGACTGTC CTATCACCGT ACTTAAACAC TACAGTATTG
    CCCAGCTCTT CTTCATCAAG GGGAAGTTTA GATAGCTCTC GTTCTGAGAA AGATAGAAGT
    CTGGAGAGAG AACGAGGTAT TAGCCTAGGC AACTATCACC CAGCAACAGA AAACCCAGGA
    ACCTCTTCAA AGCGAGGTTT GCAGATTTCT ACCACTGCAG CCCAGATTGC CAAAGTCATG
    GAAGAAGTAT CAGCTATTCA TACCTCTCAG GAAGACAGAA GTTCTGGGTC TACCACAGAA
    CTACACTGTG GGACAGATGA GAGGAATGCA CTAAGAAGAA GCTCTACCAC CCACACACAT
    GCAAACACGT ACAACTTTAC CAAGTCAGAA AACTCAAACA GAACATGTCC AATACCATAT
    GCCAAAGTAG AATATAAGAG ATCTTCAAAT GATAGTTTAA ATAGTGTCAG CAGTAGTGAT
    GGTTATGGTA AAAGAGGTCA GATGAAACCT TCAATTGAAT CCTATTCTGA AGATGATGAA
    AGTAAATTTT GCAGCTATGG TCAGTATCCA GCTGACCTAG CCCATAAAAT ACATAGTGCA
    AATCATATGG ATGATAATGA TGGAGAACTA GATACACCAA TAAATTACAG TCTCAAATAT
    TCCGATGAAC AGTTGAACTC TGGGAGGCAG AGCCCTTCAC AGAATGAAAG ATGGGCAAGA
    CCCAAACATA TACTAGAAGA TGAAATAAAA CCGAATGAGC AGAGACAATC AAGGAGTCAA
    AGCACAGCTT ATCCCGTGTA TCCTGAGAGC ACTGATGATA AACACCTCAA GTTCCAACCA
    CACTTTGGGC AGCAAGAATG TGTTTCCCCA TACAGGTCAA GAGCAGCCAA TGGATCTGAA
    ACAAATCGAG TAGGCTCTAA TCATGGAATT AGTCAAAATG TGAACCAGTC TTTGTGTCAA
    GAAGATGACT ATGAAGACGA TAAACCAACC AACTATAGCG AACGTTACTC TGAGGAAGGG
    CAGCATGAGG AAGAAGAGAG GCCAACCAAT TATAGCATAA AATACAGTGA AGAAAAACAT
    CACGTGGATC AGCCTATTGA TTATAGTTTA AAATACACCA CAGACATTCC TTCTTCACAG
    AAACCAGCAT TTTCATTCTC AAAGAATTCA TCTGGACAGA GCACAAAAAC TGAACACATC
    TCTTCAAGCA GCGAGAATAC ATCCACAACT TCATCTAATG CCAAGAGGCA GAATCAGCTG
    CATCCAAGCT CAGCACAGAG CAGAAGTGGT CAGACCCCAA AAGCCACCTC TTCCTCTTGC
    AAAGTCCCCT CTATCAACCA AGAAACAATA CAGACTTACT GTGTAGAAGA TACCCCAATA
    TGTTTTTCAA GATGCAGTTC ATTATCATCT CTGTCATCTG CTGAAGATGA AGTAGGGTGT
    GATCAGACAA CACAAGAAGC AGAGTCTGCT AACACTCTGC AAATAGCTGA AATCAAGGAC
    AACAGCGGAC CTAGGTCAAA TGAAGATTCT GTGAGTAAAG TTCCAGCAGG GTCACAGCAC
    ATTAGAACCA AATCCAGCAG ACTCCAGGCT TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC
    AAAGCTGTTG AATTTTCTTC AGGGGCCAAA TCTCCTTCCA AGAGCGGTGC TCAGACACCC
    AAAAGTCCAC CAGAGCACTA CGTTCAGGAG ACCCCACTCA TGTTTAGCAG ATGTACTTCA
    GTCAGTTCAC TCGACAGTTT TGAGAGTCGC TCGATTGCCA GCTCTGTTCA GAGTGAACCC
    TGCAGTGGAA TGGTAAGTGG CATCATAAGC CCCAGTGACC TCCCAGATAG CCCTGGACAA
    ACCATGCCAC CAAGCAGAAG CAAAACCCCT CCGCCACCGC CTCCGCCTCC TCCTCAGACA
    GTTCAAACGA AGCAGGAAGT ACCTAAAAAT AAAGCACCTA GTGCTGAAAA GAGAGAAAGT
    GGACCCAAGC AAGCTGCTGT AAATGCTGCA GTACAAAGGG TCCAGGTTCT TCCAGAGGCT
    GATACTCTGT TACATTTTGC CACAGAGAGT ACTCCTGATG GATTTTCTTG TTCATCTAGC
    CTGAGCGCTC TGAGCCTTGA TGAGCCATTT ATTCAGAAAG ATGTGGAACT AAGAATAATG
    CCTCCAGTTC AGGAAAATGA CAACGGGAAT GAAACAGAAA GTGAGCAGCC TGAAGAATCA
    AATGAAAACC AGGAAAAAGA GGCAGAAAAA CCCACTGATT CTGAAAAAGA TCTTTTAGAT
    GAGTCAGATG ATGATGATAT TGAAATACTA GAAGAGTGTA TAATTTCTGC CATGCCAACA
    AAATCTTCAC GCAAAGCCAA AAAACCAGCC CAGACTACTT CAAAATTACC TCCACCTGTG
    GCAAGGAAAC CAAGTCAGCT CCCTGTATAC AAACTTCTGC CATCGCAAAA CAGGTTACAG
    GCACAAAAGC ATGTTAGTTT TACACCAGGA GATGATATGC CACGGGTGTA TTGTGTAGAA
    GGGACACCTA TAAACTTTTC CACAGCTACA TCTCTGAGTG ATCTAACGAT AGAATCCCCT
    CCAAATGAGT TAGCTGCTGG AGAAGGGGTT AGAGCAGGGG CACAGTCAAG TGAGTTTGAA
    AAACGAGATA CCATTCCTAC TGAAGGCAGA AGTACAGATG AGGCTCAACG AGGGAAAGCC
    TCATCTGTCA CTGTGCCTGA ACTGGACGAC AGCAAAACAG AAGAAGGTGA TATTCTTGCA
    GAATGCATTA ATTCTGCCAT GCCCAAAGGA AAAAGTCACA AGCCTTTCCG TGTGAAAAAG
    ATCATGGACC AGGTCCAGCA AGCATCTATG TCTTCATCTG GAACTAACAA AAATCAATTA
    GATGGTAAGA CGAAGAAACC TACTTCACCA GTAAAACCTA TACCACAAAA TACTGAATAC
    AGGACACGTG TAAGAAAAAA TACAGACTCA AAAAATAATC TAAATGCAGA AAGAAATTTC
    TCGGAAAACA AAGACTCAAA GAAACAGCAC TTGAAAAATA ATTCCAAGGA CTTCAATGAT
    AAACTGCCAA ATAATGAAGA CAGAGTCAGA GGAAGTTTTA CTTTTGATTC ACCCCATCAT
    TACACGCCCA TTGAAGGCAC TCCGTACTGT TTTTCACGAA ATGACTCTCT GAGTTCTCTA
    GATTTTGATG ATGATGATGT TGACCTTTCC AGGGAAAAGG CTGAATTAAG AAAGGGGAAG
    GAAAATAAAG AATCAGAAGC TAAAGTGACC AACCACACAG AACTAACCTC AAACCAACAA
    TCAGCTAGTA AGACACCAGC TGTTACAAAG CAGCCAATAA ATAGAGGTCA GTCTAAACCC
    GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG TCTTCCAAAG ATATACCAGA CAGAGGGGCA
    GCAACAGATG AGAAATTACA GAATTTCGCT ATTGAAAATA CTCCAGTTTG CTTTTCTCGA
    AATTCCTCTC TAAGCTCTCT TAGTGACATT GATCAAGAAA ACAACAACAA CAAGGAAAAT
    GAACCTGTCA AAGAGACAGA GCCCCCTGCC TCACAGGGAG AACCAGGTAA ACCCCAGGCC
    TCAGGTTATG CTCCTAAATC GTTTCACGTG GAAGACACCC CTGTTTGTTT CTCAAGAAAC
    AGTTCTCTCA GTTCTCTTAG TATTGATTCT GAAGATGACC TGCTGCAGGA ATGTATAAGT
    TCTGCAATGC CAAAAAAGAA AAAGCCTTCA AGGCTCAAGC CCGATAATGA AAAGCATAGT
    CCCAGAAATA TGGGTGGCAT ATTAGCAGAA GATTTGACAC TCGATTTGAA AGATATACAG
    AGACCAGATT CAGAACATGG TTTATCCCCA GATTCAGAAA ATTTTGATTG GAAAGCTATT
    CAGGAAGGTG CAAATTCCAT AGTAAGTAGT TTACATCAAG CTGCTGCCGC TGCATGTTTA
    TCTCGACAAG CTTCATCTGA TTCAGATTCT ATCCTTTCAT TGAAATCGGG TATCTCTCTG
    GGATCACCAT TTCACCTTAC ACCTGATCAA GAAGAAAAAC CCTTTACGAG TAATAAAGGT
    CCACGAATTC TAAAACCTGG GGAGAAAAGT ACATTGGAAA CTAAAAAAAT AGAATCTGAA
    AATAAAGGAA TAAAAGGAGG CAAAAAGGTT TATAAAAGTT TGATTACTGG AAAAGTTCGA
    TCTAATTCGG AAATTTCGAG CCAAATGAAA CAACCCCTTC AAACAAACAT GCCTTCAATC
    TCTCGAGGTA GGACAATGAT TCATATTCCA GGAGTTCGGA ATAGCTCTTC AAGTACAAGC
    CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT
    CAGCCGGCTA CCACCTCTCC CAGAGGAACC AAGCCATCAG TGAAGTCAGA ATTAAGCCCT
    GTTACAAGGC AGGCATCCCA GACAGCAGGA TCAAACAAAG GACCTTCTAG ATCAGGATCT
    AGAGATTCCA CTCCTTCAAG ACCTGCCCAG CAACCATTAA GTAGACCAAT GCAGTCTCCA
    GGGCGAAACT CAATCTCTCC TGGTAGAAAT GGAATAAGTC CCCCTAACAA ATTATCTCAA
    CTACCAAGGA CGTCATCCCC TAGTACTGCT TCAACTAAGT CCTCAGGTTC TGGGAAAATG
    TCTTACACAT CTCCTGGCAG ACAGATGAGC CAGCAGAACC TCACCAAACA AACAGGCTTA
    TCCAAGAATG GCAGTGGTAT CCCAAGAAGT GAATCTGCCT CCAAAGGGCT AAATCAAATG
    AGTAATAGTA ATGGATCCAA TAAAAAAGTA GAACTTTCTA GAATGTCTTC AACAAAGTCA
    AGTGGAAGTG AATCCGATAG GTCAGAGAGA CCTGTATTAG TACGCCAATC AACTTTCATC
    AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA AAACTGGAGG AATCTGCTTC ATTTGAATCT
    CTTTCTCCAT CTTCCAGACC CGATTCTCCC ACACGGTCCC AGGCTCATAC TCCAGTTTTA
    AGTCCTTCCC TTCCTGATAT GTCTCTATCT ACACATTCGT CTCTTCAGTC TGGTGGATGG
    CGAAAACTCC CACCTAACCT CAGTCCCACC ATAGAGTATA ATGATGGAAG ACCAGTAAAG
    CGCCATGATA TAGCACGCTC TCATTCCGAA AGTCCTTCCA GACTTCCCAT CAATAGGTCA
    GGGACCTGGA AACGTGAGCA CAGCAAACAC TCATCATCAC TTCCTCGAGT AAGCACTTGG
    AGAAGAACTG GAAGTTCATC CTCAATTCTT TCTGCTTCAT CAGAATCTAG TGAAAAGGCA
    AAAAGTGAGG ACGAAAAACA AGTGAACTCT ATTTCAGGAA GCAAACAAAC TAAAGAAAAC
    CAGGTATCCA CAAAAGGAAC ATGGAGAAAA ATAAAAGAAA GTGAAATTTC TCCCACAAAT
    AGTACTTCTC AGACCACTTC TTCAGGTGCT GCAAATGGTG CTGAATCAAA GACTCTGATT
    TATCAAATGG CACCTGCTGT TTCTAAAACA GAGGATGTTT GGGTGAGAAT TGAGGATTGC
    CCCATTAACA ACCCTAGATC TGGAAGATCT CCAACAGGAA ATACTCCCCC CGTGATTGAC
    ACTGTTTCAG AAAAGGGAAA CCCGAACCCT AAAGATTCAA AAGATAATCA GGGAAAACAA
    AATGTGAGCA ATGGTAGTGC CCCTACACGC ACCATGGGTC TGGAAAACCG CCTGAATTCC
    TTTATTCAGG TAGATCCTCC AGACCAAAAA GGAACTGAGA CAAAACCGGG ACACAGTAAT
    AACCCTGTCC CTGCATCCGA GACTAGTGAA AGTTCTATAG CCGAGCGTAC CCCGTTTAGT
    TCTAGCAGCT CAAGCAAGCA CAGTTCACCA AGTGGGACTG TCGCCGCCAG GGTGAGTCCT
    TTTAATTACA ACCCAAGCCC AAGGAAGAGC AGCACAGATG GCACTTCAGC CCGACCGTCT
    CAGATCCCAA CGCCAGTGAG CAACAACACA AAGAAACGCG ACTCAAAACC CGACAGCACA
    GAACCCAGCG GGACTCAAAG TCCTAAACGC CATTCTGGGT CTTACCTTGT GACATCTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211259.1
    CDS132..8414
    Translation

    Target ORF information:

    RefSeq Version XM_005211202.4
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211202.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGGGGA
    CTACAGGCCA TTGCAGAATT ATTGCAAGTG GACTGTGAAA TGTATGGGCT TACTAATGAC
    CACTACAGTA TTACTTTAAG ACGATATGCA GGAATGGCTT TGACAAACTT GACTTTCGGA
    GATGTAGCCA ACAAGGCTAC TCTCTGCTCT ATGAAAGGCT GCATGAGAGC GCTCGTGGCT
    CAGCTCCAGT CTGAAAGCGA GGACTTGCAG CAGGTTATTG CGAGTGTTTT GAGGAATCTG
    TCTTGGAGAG CAGATGTAAA TAGTAAAAAG ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA
    TTGATGGAAT GTGCTTTGGA AGTGAAAAAG GAATCAACCC TCAAAAGTGT ATTGAGTGCC
    TTATGGAACT TGTCAGCACA CTGCACTGAG AATAAAGCTG ACATATGTGC TGTGGATGGT
    GCGCTTGCAT TTTTGGTTGG CACTCTCACT TATCGCAGCC AGACCAATAC TTTAGCCATT
    ATTGAAAGTG GAGGTGGTAT ATTACGGAAT GTATCCAGCT TGATAGCTAC AAATGAGGAC
    CACAGGCAAA TCCTAAGAGA GAATAATTGC TTACAGACCT TATTACAACA CTTGAAATCT
    CACAGTTTGA CAATAGTCAG CAATGCATGC GGAACCTTGT GGAATCTCTC AGCAAGAAAC
    CCTAAAGACC AGGAAGCATT GTGGGACATG GGAGCAGTCA GCATGCTCAA GAACCTCATT
    CATTCAAAGC ACAAGATGAT TGCTATGGGA AGTGCTGCAG CTTTAAGGAA TCTCATGGCA
    AATAGACCTG CAAAGTATAA AGATGCCAAT ATCATGTCTC CTGGTTCAAG TTTGCCTTCT
    CTACATGTCA GGAAACAAAA GGCCCTGGAA GCAGAATTAG ATGCTCAGCA TTTATCAGAA
    ACTTTTGACA ATATTGACAA TTTAAGTCCC AAGGCATCTC ATCGTAGCAA GCAGAGACAC
    AAGCAAAATC TCTATGGTGA CTATGTTTTT GACACCAATC GACATGATGA TAACAGGTCA
    GACAATTTTA ATACTGGAAA CATGACTGTC CTATCACCGT ACTTAAACAC TACAGTATTG
    CCCAGCTCTT CTTCATCAAG GGGAAGTTTA GATAGCTCTC GTTCTGAGAA AGATAGAAGT
    CTGGAGAGAG AACGAGGTAT TAGCCTAGGC AACTATCACC CAGCAACAGA AAACCCAGGA
    ACCTCTTCAA AGCGAGGTTT GCAGATTTCT ACCACTGCAG CCCAGATTGC CAAAGTCATG
    GAAGAAGTAT CAGCTATTCA TACCTCTCAG GAAGACAGAA GTTCTGGGTC TACCACAGAA
    CTACACTGTG GGACAGATGA GAGGAATGCA CTAAGAAGAA GCTCTACCAC CCACACACAT
    GCAAACACGT ACAACTTTAC CAAGTCAGAA AACTCAAACA GAACATGTCC AATACCATAT
    GCCAAAGTAG AATATAAGAG ATCTTCAAAT GATAGTTTAA ATAGTGTCAG CAGTAGTGAT
    GGTTATGGTA AAAGAGGTCA GATGAAACCT TCAATTGAAT CCTATTCTGA AGATGATGAA
    AGTAAATTTT GCAGCTATGG TCAGTATCCA GCTGACCTAG CCCATAAAAT ACATAGTGCA
    AATCATATGG ATGATAATGA TGGAGAACTA GATACACCAA TAAATTACAG TCTCAAATAT
    TCCGATGAAC AGTTGAACTC TGGGAGGCAG AGCCCTTCAC AGAATGAAAG ATGGGCAAGA
    CCCAAACATA TACTAGAAGA TGAAATAAAA CCGAATGAGC AGAGACAATC AAGGAGTCAA
    AGCACAGCTT ATCCCGTGTA TCCTGAGAGC ACTGATGATA AACACCTCAA GTTCCAACCA
    CACTTTGGGC AGCAAGAATG TGTTTCCCCA TACAGGTCAA GAGCAGCCAA TGGATCTGAA
    ACAAATCGAG TAGGCTCTAA TCATGGAATT AGTCAAAATG TGAACCAGTC TTTGTGTCAA
    GAAGATGACT ATGAAGACGA TAAACCAACC AACTATAGCG AACGTTACTC TGAGGAAGGG
    CAGCATGAGG AAGAAGAGAG GCCAACCAAT TATAGCATAA AATACAGTGA AGAAAAACAT
    CACGTGGATC AGCCTATTGA TTATAGTTTA AAATACACCA CAGACATTCC TTCTTCACAG
    AAACCAGCAT TTTCATTCTC AAAGAATTCA TCTGGACAGA GCACAAAAAC TGAACACATC
    TCTTCAAGCA GCGAGAATAC ATCCACAACT TCATCTAATG CCAAGAGGCA GAATCAGCTG
    CATCCAAGCT CAGCACAGAG CAGAAGTGGT CAGACCCCAA AAGCCACCTC TTCCTCTTGC
    AAAGTCCCCT CTATCAACCA AGAAACAATA CAGACTTACT GTGTAGAAGA TACCCCAATA
    TGTTTTTCAA GATGCAGTTC ATTATCATCT CTGTCATCTG CTGAAGATGA AGTAGGGTGT
    GATCAGACAA CACAAGAAGC AGAGTCTGCT AACACTCTGC AAATAGCTGA AATCAAGGAC
    AACAGCGGAC CTAGGTCAAA TGAAGATTCT GTGAGTAAAG TTCCAGCAGG GTCACAGCAC
    ATTAGAACCA AATCCAGCAG ACTCCAGGCT TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC
    AAAGCTGTTG AATTTTCTTC AGGGGCCAAA TCTCCTTCCA AGAGCGGTGC TCAGACACCC
    AAAAGTCCAC CAGAGCACTA CGTTCAGGAG ACCCCACTCA TGTTTAGCAG ATGTACTTCA
    GTCAGTTCAC TCGACAGTTT TGAGAGTCGC TCGATTGCCA GCTCTGTTCA GAGTGAACCC
    TGCAGTGGAA TGGTAAGTGG CATCATAAGC CCCAGTGACC TCCCAGATAG CCCTGGACAA
    ACCATGCCAC CAAGCAGAAG CAAAACCCCT CCGCCACCGC CTCCGCCTCC TCCTCAGACA
    GTTCAAACGA AGCAGGAAGT ACCTAAAAAT AAAGCACCTA GTGCTGAAAA GAGAGAAAGT
    GGACCCAAGC AAGCTGCTGT AAATGCTGCA GTACAAAGGG TCCAGGTTCT TCCAGAGGCT
    GATACTCTGT TACATTTTGC CACAGAGAGT ACTCCTGATG GATTTTCTTG TTCATCTAGC
    CTGAGCGCTC TGAGCCTTGA TGAGCCATTT ATTCAGAAAG ATGTGGAACT AAGAATAATG
    CCTCCAGTTC AGGAAAATGA CAACGGGAAT GAAACAGAAA GTGAGCAGCC TGAAGAATCA
    AATGAAAACC AGGAAAAAGA GGCAGAAAAA CCCACTGATT CTGAAAAAGA TCTTTTAGAT
    GAGTCAGATG ATGATGATAT TGAAATACTA GAAGAGTGTA TAATTTCTGC CATGCCAACA
    AAATCTTCAC GCAAAGCCAA AAAACCAGCC CAGACTACTT CAAAATTACC TCCACCTGTG
    GCAAGGAAAC CAAGTCAGCT CCCTGTATAC AAACTTCTGC CATCGCAAAA CAGGTTACAG
    GCACAAAAGC ATGTTAGTTT TACACCAGGA GATGATATGC CACGGGTGTA TTGTGTAGAA
    GGGACACCTA TAAACTTTTC CACAGCTACA TCTCTGAGTG ATCTAACGAT AGAATCCCCT
    CCAAATGAGT TAGCTGCTGG AGAAGGGGTT AGAGCAGGGG CACAGTCAAG TGAGTTTGAA
    AAACGAGATA CCATTCCTAC TGAAGGCAGA AGTACAGATG AGGCTCAACG AGGGAAAGCC
    TCATCTGTCA CTGTGCCTGA ACTGGACGAC AGCAAAACAG AAGAAGGTGA TATTCTTGCA
    GAATGCATTA ATTCTGCCAT GCCCAAAGGA AAAAGTCACA AGCCTTTCCG TGTGAAAAAG
    ATCATGGACC AGGTCCAGCA AGCATCTATG TCTTCATCTG GAACTAACAA AAATCAATTA
    GATGGTAAGA CGAAGAAACC TACTTCACCA GTAAAACCTA TACCACAAAA TACTGAATAC
    AGGACACGTG TAAGAAAAAA TACAGACTCA AAAAATAATC TAAATGCAGA AAGAAATTTC
    TCGGAAAACA AAGACTCAAA GAAACAGCAC TTGAAAAATA ATTCCAAGGA CTTCAATGAT
    AAACTGCCAA ATAATGAAGA CAGAGTCAGA GGAAGTTTTA CTTTTGATTC ACCCCATCAT
    TACACGCCCA TTGAAGGCAC TCCGTACTGT TTTTCACGAA ATGACTCTCT GAGTTCTCTA
    GATTTTGATG ATGATGATGT TGACCTTTCC AGGGAAAAGG CTGAATTAAG AAAGGGGAAG
    GAAAATAAAG AATCAGAAGC TAAAGTGACC AACCACACAG AACTAACCTC AAACCAACAA
    TCAGCTAGTA AGACACCAGC TGTTACAAAG CAGCCAATAA ATAGAGGTCA GTCTAAACCC
    GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG TCTTCCAAAG ATATACCAGA CAGAGGGGCA
    GCAACAGATG AGAAATTACA GAATTTCGCT ATTGAAAATA CTCCAGTTTG CTTTTCTCGA
    AATTCCTCTC TAAGCTCTCT TAGTGACATT GATCAAGAAA ACAACAACAA CAAGGAAAAT
    GAACCTGTCA AAGAGACAGA GCCCCCTGCC TCACAGGGAG AACCAGGTAA ACCCCAGGCC
    TCAGGTTATG CTCCTAAATC GTTTCACGTG GAAGACACCC CTGTTTGTTT CTCAAGAAAC
    AGTTCTCTCA GTTCTCTTAG TATTGATTCT GAAGATGACC TGCTGCAGGA ATGTATAAGT
    TCTGCAATGC CAAAAAAGAA AAAGCCTTCA AGGCTCAAGC CCGATAATGA AAAGCATAGT
    CCCAGAAATA TGGGTGGCAT ATTAGCAGAA GATTTGACAC TCGATTTGAA AGATATACAG
    AGACCAGATT CAGAACATGG TTTATCCCCA GATTCAGAAA ATTTTGATTG GAAAGCTATT
    CAGGAAGGTG CAAATTCCAT AGTAAGTAGT TTACATCAAG CTGCTGCCGC TGCATGTTTA
    TCTCGACAAG CTTCATCTGA TTCAGATTCT ATCCTTTCAT TGAAATCGGG TATCTCTCTG
    GGATCACCAT TTCACCTTAC ACCTGATCAA GAAGAAAAAC CCTTTACGAG TAATAAAGGT
    CCACGAATTC TAAAACCTGG GGAGAAAAGT ACATTGGAAA CTAAAAAAAT AGAATCTGAA
    AATAAAGGAA TAAAAGGAGG CAAAAAGGTT TATAAAAGTT TGATTACTGG AAAAGTTCGA
    TCTAATTCGG AAATTTCGAG CCAAATGAAA CAACCCCTTC AAACAAACAT GCCTTCAATC
    TCTCGAGGTA GGACAATGAT TCATATTCCA GGAGTTCGGA ATAGCTCTTC AAGTACAAGC
    CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT
    CAGCCGGCTA CCACCTCTCC CAGAGGAACC AAGCCATCAG TGAAGTCAGA ATTAAGCCCT
    GTTACAAGGC AGGCATCCCA GACAGCAGGA TCAAACAAAG GACCTTCTAG ATCAGGATCT
    AGAGATTCCA CTCCTTCAAG ACCTGCCCAG CAACCATTAA GTAGACCAAT GCAGTCTCCA
    GGGCGAAACT CAATCTCTCC TGGTAGAAAT GGAATAAGTC CCCCTAACAA ATTATCTCAA
    CTACCAAGGA CGTCATCCCC TAGTACTGCT TCAACTAAGT CCTCAGGTTC TGGGAAAATG
    TCTTACACAT CTCCTGGCAG ACAGATGAGC CAGCAGAACC TCACCAAACA AACAGGCTTA
    TCCAAGAATG GCAGTGGTAT CCCAAGAAGT GAATCTGCCT CCAAAGGGCT AAATCAAATG
    AGTAATAGTA ATGGATCCAA TAAAAAAGTA GAACTTTCTA GAATGTCTTC AACAAAGTCA
    AGTGGAAGTG AATCCGATAG GTCAGAGAGA CCTGTATTAG TACGCCAATC AACTTTCATC
    AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA AAACTGGAGG AATCTGCTTC ATTTGAATCT
    CTTTCTCCAT CTTCCAGACC CGATTCTCCC ACACGGTCCC AGGCTCATAC TCCAGTTTTA
    AGTCCTTCCC TTCCTGATAT GTCTCTATCT ACACATTCGT CTCTTCAGTC TGGTGGATGG
    CGAAAACTCC CACCTAACCT CAGTCCCACC ATAGAGTATA ATGATGGAAG ACCAGTAAAG
    CGCCATGATA TAGCACGCTC TCATTCCGAA AGTCCTTCCA GACTTCCCAT CAATAGGTCA
    GGGACCTGGA AACGTGAGCA CAGCAAACAC TCATCATCAC TTCCTCGAGT AAGCACTTGG
    AGAAGAACTG GAAGTTCATC CTCAATTCTT TCTGCTTCAT CAGAATCTAG TGAAAAGGCA
    AAAAGTGAGG ACGAAAAACA AGTGAACTCT ATTTCAGGAA GCAAACAAAC TAAAGAAAAC
    CAGGTATCCA CAAAAGGAAC ATGGAGAAAA ATAAAAGAAA GTGAAATTTC TCCCACAAAT
    AGTACTTCTC AGACCACTTC TTCAGGTGCT GCAAATGGTG CTGAATCAAA GACTCTGATT
    TATCAAATGG CACCTGCTGT TTCTAAAACA GAGGATGTTT GGGTGAGAAT TGAGGATTGC
    CCCATTAACA ACCCTAGATC TGGAAGATCT CCAACAGGAA ATACTCCCCC CGTGATTGAC
    ACTGTTTCAG AAAAGGGAAA CCCGAACCCT AAAGATTCAA AAGATAATCA GGGAAAACAA
    AATGTGAGCA ATGGTAGTGC CCCTACACGC ACCATGGGTC TGGAAAACCG CCTGAATTCC
    TTTATTCAGG TAGATCCTCC AGACCAAAAA GGAACTGAGA CAAAACCGGG ACACAGTAAT
    AACCCTGTCC CTGCATCCGA GACTAGTGAA AGTTCTATAG CCGAGCGTAC CCCGTTTAGT
    TCTAGCAGCT CAAGCAAGCA CAGTTCACCA AGTGGGACTG TCGCCGCCAG GGTGAGTCCT
    TTTAATTACA ACCCAAGCCC AAGGAAGAGC AGCACAGATG GCACTTCAGC CCGACCGTCT
    CAGATCCCAA CGCCAGTGAG CAACAACACA AAGAAACGCG ACTCAAAACC CGACAGCACA
    GAACCCAGCG GGACTCAAAG TCCTAAACGC CATTCTGGGT CTTACCTTGT GACATCTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219621
    Clone ID Related Accession (Same CDS sequence) XM_005211203.4
    Accession Version XM_005211203.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211203.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGATACGAG CTTACTGTGA AACCTGTTGG
    GAGTGGCAGG AAGCCCATGA ACAAGGGATG GACCAGGACA AAAATCCAAT GCCAGCTCCT
    GTTGAACATC AGATCTGCCC TGCTGTCTGT GTTCTAATGA AGCTTTCGTT TGATGAAGAG
    CATAGACATG CCATGAATGA GCTTGGGGGA CTACAGGCCA TTGCAGAATT ATTGCAAGTG
    GACTGTGAAA TGTATGGGCT TACTAATGAC CACTACAGTA TTACTTTAAG ACGATATGCA
    GGAATGGCTT TGACAAACTT GACTTTCGGA GATGTAGCCA ACAAGGCTAC TCTCTGCTCT
    ATGAAAGGCT GCATGAGAGC GCTCGTGGCT CAGCTCCAGT CTGAAAGCGA GGACTTGCAG
    CAGGTTATTG CGAGTGTTTT GAGGAATCTG TCTTGGAGAG CAGATGTAAA TAGTAAAAAG
    ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTGAAAAAG
    GAATCAACCC TCAAAAGTGT ATTGAGTGCC TTATGGAACT TGTCAGCACA CTGCACTGAG
    AATAAAGCTG ACATATGTGC TGTGGATGGT GCGCTTGCAT TTTTGGTTGG CACTCTCACT
    TATCGCAGCC AGACCAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGTAT ATTACGGAAT
    GTATCCAGCT TGATAGCTAC AAATGAGGAC CACAGGCAAA TCCTAAGAGA GAATAATTGC
    TTACAGACCT TATTACAACA CTTGAAATCT CACAGTTTGA CAATAGTCAG CAATGCATGC
    GGAACCTTGT GGAATCTCTC AGCAAGAAAC CCTAAAGACC AGGAAGCATT GTGGGACATG
    GGAGCAGTCA GCATGCTCAA GAACCTCATT CATTCAAAGC ACAAGATGAT TGCTATGGGA
    AGTGCTGCAG CTTTAAGGAA TCTCATGGCA AATAGACCTG CAAAGTATAA AGATGCCAAT
    ATCATGTCTC CTGGTTCAAG TTTGCCTTCT CTACATGTCA GGAAACAAAA GGCCCTGGAA
    GCAGAATTAG ATGCTCAGCA TTTATCAGAA ACTTTTGACA ATATTGACAA TTTAAGTCCC
    AAGGCATCTC ATCGTAGCAA GCAGAGACAC AAGCAAAATC TCTATGGTGA CTATGTTTTT
    GACACCAATC GACATGATGA TAACAGGTCA GACAATTTTA ATACTGGAAA CATGACTGTC
    CTATCACCGT ACTTAAACAC TACAGTATTG CCCAGCTCTT CTTCATCAAG GGGAAGTTTA
    GATAGCTCTC GTTCTGAGAA AGATAGAAGT CTGGAGAGAG AACGAGGTAT TAGCCTAGGC
    AACTATCACC CAGCAACAGA AAACCCAGGA ACCTCTTCAA AGCGAGGTTT GCAGATTTCT
    ACCACTGCAG CCCAGATTGC CAAAGTCATG GAAGAAGTAT CAGCTATTCA TACCTCTCAG
    GAAGACAGAA GTTCTGGGTC TACCACAGAA CTACACTGTG GGACAGATGA GAGGAATGCA
    CTAAGAAGAA GCTCTACCAC CCACACACAT GCAAACACGT ACAACTTTAC CAAGTCAGAA
    AACTCAAACA GAACATGTCC AATACCATAT GCCAAAGTAG AATATAAGAG ATCTTCAAAT
    GATAGTTTAA ATAGTGTCAG CAGTAGTGAT GGTTATGGTA AAAGAGGTCA GATGAAACCT
    TCAATTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGCTATGG TCAGTATCCA
    GCTGACCTAG CCCATAAAAT ACATAGTGCA AATCATATGG ATGATAATGA TGGAGAACTA
    GATACACCAA TAAATTACAG TCTCAAATAT TCCGATGAAC AGTTGAACTC TGGGAGGCAG
    AGCCCTTCAC AGAATGAAAG ATGGGCAAGA CCCAAACATA TACTAGAAGA TGAAATAAAA
    CCGAATGAGC AGAGACAATC AAGGAGTCAA AGCACAGCTT ATCCCGTGTA TCCTGAGAGC
    ACTGATGATA AACACCTCAA GTTCCAACCA CACTTTGGGC AGCAAGAATG TGTTTCCCCA
    TACAGGTCAA GAGCAGCCAA TGGATCTGAA ACAAATCGAG TAGGCTCTAA TCATGGAATT
    AGTCAAAATG TGAACCAGTC TTTGTGTCAA GAAGATGACT ATGAAGACGA TAAACCAACC
    AACTATAGCG AACGTTACTC TGAGGAAGGG CAGCATGAGG AAGAAGAGAG GCCAACCAAT
    TATAGCATAA AATACAGTGA AGAAAAACAT CACGTGGATC AGCCTATTGA TTATAGTTTA
    AAATACACCA CAGACATTCC TTCTTCACAG AAACCAGCAT TTTCATTCTC AAAGAATTCA
    TCTGGACAGA GCACAAAAAC TGAACACATC TCTTCAAGCA GCGAGAATAC ATCCACAACT
    TCATCTAATG CCAAGAGGCA GAATCAGCTG CATCCAAGCT CAGCACAGAG CAGAAGTGGT
    CAGACCCCAA AAGCCACCTC TTCCTCTTGC AAAGTCCCCT CTATCAACCA AGAAACAATA
    CAGACTTACT GTGTAGAAGA TACCCCAATA TGTTTTTCAA GATGCAGTTC ATTATCATCT
    CTGTCATCTG CTGAAGATGA AGTAGGGTGT GATCAGACAA CACAAGAAGC AGAGTCTGCT
    AACACTCTGC AAATAGCTGA AATCAAGGAC AACAGCGGAC CTAGGTCAAA TGAAGATTCT
    GTGAGTAAAG TTCCAGCAGG GTCACAGCAC ATTAGAACCA AATCCAGCAG ACTCCAGGCT
    TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC AAAGCTGTTG AATTTTCTTC AGGGGCCAAA
    TCTCCTTCCA AGAGCGGTGC TCAGACACCC AAAAGTCCAC CAGAGCACTA CGTTCAGGAG
    ACCCCACTCA TGTTTAGCAG ATGTACTTCA GTCAGTTCAC TCGACAGTTT TGAGAGTCGC
    TCGATTGCCA GCTCTGTTCA GAGTGAACCC TGCAGTGGAA TGGTAAGTGG CATCATAAGC
    CCCAGTGACC TCCCAGATAG CCCTGGACAA ACCATGCCAC CAAGCAGAAG CAAAACCCCT
    CCGCCACCGC CTCCGCCTCC TCCTCAGACA GTTCAAACGA AGCAGGAAGT ACCTAAAAAT
    AAAGCACCTA GTGCTGAAAA GAGAGAAAGT GGACCCAAGC AAGCTGCTGT AAATGCTGCA
    GTACAAAGGG TCCAGGTTCT TCCAGAGGCT GATACTCTGT TACATTTTGC CACAGAGAGT
    ACTCCTGATG GATTTTCTTG TTCATCTAGC CTGAGCGCTC TGAGCCTTGA TGAGCCATTT
    ATTCAGAAAG ATGTGGAACT AAGAATAATG CCTCCAGTTC AGGAAAATGA CAACGGGAAT
    GAAACAGAAA GTGAGCAGCC TGAAGAATCA AATGAAAACC AGGAAAAAGA GGCAGAAAAA
    CCCACTGATT CTGAAAAAGA TCTTTTAGAT GAGTCAGATG ATGATGATAT TGAAATACTA
    GAAGAGTGTA TAATTTCTGC CATGCCAACA AAATCTTCAC GCAAAGCCAA AAAACCAGCC
    CAGACTACTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT CCCTGTATAC
    AAACTTCTGC CATCGCAAAA CAGGTTACAG GCACAAAAGC ATGTTAGTTT TACACCAGGA
    GATGATATGC CACGGGTGTA TTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCTACA
    TCTCTGAGTG ATCTAACGAT AGAATCCCCT CCAAATGAGT TAGCTGCTGG AGAAGGGGTT
    AGAGCAGGGG CACAGTCAAG TGAGTTTGAA AAACGAGATA CCATTCCTAC TGAAGGCAGA
    AGTACAGATG AGGCTCAACG AGGGAAAGCC TCATCTGTCA CTGTGCCTGA ACTGGACGAC
    AGCAAAACAG AAGAAGGTGA TATTCTTGCA GAATGCATTA ATTCTGCCAT GCCCAAAGGA
    AAAAGTCACA AGCCTTTCCG TGTGAAAAAG ATCATGGACC AGGTCCAGCA AGCATCTATG
    TCTTCATCTG GAACTAACAA AAATCAATTA GATGGTAAGA CGAAGAAACC TACTTCACCA
    GTAAAACCTA TACCACAAAA TACTGAATAC AGGACACGTG TAAGAAAAAA TACAGACTCA
    AAAAATAATC TAAATGCAGA AAGAAATTTC TCGGAAAACA AAGACTCAAA GAAACAGCAC
    TTGAAAAATA ATTCCAAGGA CTTCAATGAT AAACTGCCAA ATAATGAAGA CAGAGTCAGA
    GGAAGTTTTA CTTTTGATTC ACCCCATCAT TACACGCCCA TTGAAGGCAC TCCGTACTGT
    TTTTCACGAA ATGACTCTCT GAGTTCTCTA GATTTTGATG ATGATGATGT TGACCTTTCC
    AGGGAAAAGG CTGAATTAAG AAAGGGGAAG GAAAATAAAG AATCAGAAGC TAAAGTGACC
    AACCACACAG AACTAACCTC AAACCAACAA TCAGCTAGTA AGACACCAGC TGTTACAAAG
    CAGCCAATAA ATAGAGGTCA GTCTAAACCC GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG
    TCTTCCAAAG ATATACCAGA CAGAGGGGCA GCAACAGATG AGAAATTACA GAATTTCGCT
    ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TAAGCTCTCT TAGTGACATT
    GATCAAGAAA ACAACAACAA CAAGGAAAAT GAACCTGTCA AAGAGACAGA GCCCCCTGCC
    TCACAGGGAG AACCAGGTAA ACCCCAGGCC TCAGGTTATG CTCCTAAATC GTTTCACGTG
    GAAGACACCC CTGTTTGTTT CTCAAGAAAC AGTTCTCTCA GTTCTCTTAG TATTGATTCT
    GAAGATGACC TGCTGCAGGA ATGTATAAGT TCTGCAATGC CAAAAAAGAA AAAGCCTTCA
    AGGCTCAAGC CCGATAATGA AAAGCATAGT CCCAGAAATA TGGGTGGCAT ATTAGCAGAA
    GATTTGACAC TCGATTTGAA AGATATACAG AGACCAGATT CAGAACATGG TTTATCCCCA
    GATTCAGAAA ATTTTGATTG GAAAGCTATT CAGGAAGGTG CAAATTCCAT AGTAAGTAGT
    TTACATCAAG CTGCTGCCGC TGCATGTTTA TCTCGACAAG CTTCATCTGA TTCAGATTCT
    ATCCTTTCAT TGAAATCGGG TATCTCTCTG GGATCACCAT TTCACCTTAC ACCTGATCAA
    GAAGAAAAAC CCTTTACGAG TAATAAAGGT CCACGAATTC TAAAACCTGG GGAGAAAAGT
    ACATTGGAAA CTAAAAAAAT AGAATCTGAA AATAAAGGAA TAAAAGGAGG CAAAAAGGTT
    TATAAAAGTT TGATTACTGG AAAAGTTCGA TCTAATTCGG AAATTTCGAG CCAAATGAAA
    CAACCCCTTC AAACAAACAT GCCTTCAATC TCTCGAGGTA GGACAATGAT TCATATTCCA
    GGAGTTCGGA ATAGCTCTTC AAGTACAAGC CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG
    ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT CAGCCGGCTA CCACCTCTCC CAGAGGAACC
    AAGCCATCAG TGAAGTCAGA ATTAAGCCCT GTTACAAGGC AGGCATCCCA GACAGCAGGA
    TCAAACAAAG GACCTTCTAG ATCAGGATCT AGAGATTCCA CTCCTTCAAG ACCTGCCCAG
    CAACCATTAA GTAGACCAAT GCAGTCTCCA GGGCGAAACT CAATCTCTCC TGGTAGAAAT
    GGAATAAGTC CCCCTAACAA ATTATCTCAA CTACCAAGGA CGTCATCCCC TAGTACTGCT
    TCAACTAAGT CCTCAGGTTC TGGGAAAATG TCTTACACAT CTCCTGGCAG ACAGATGAGC
    CAGCAGAACC TCACCAAACA AACAGGCTTA TCCAAGAATG GCAGTGGTAT CCCAAGAAGT
    GAATCTGCCT CCAAAGGGCT AAATCAAATG AGTAATAGTA ATGGATCCAA TAAAAAAGTA
    GAACTTTCTA GAATGTCTTC AACAAAGTCA AGTGGAAGTG AATCCGATAG GTCAGAGAGA
    CCTGTATTAG TACGCCAATC AACTTTCATC AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA
    AAACTGGAGG AATCTGCTTC ATTTGAATCT CTTTCTCCAT CTTCCAGACC CGATTCTCCC
    ACACGGTCCC AGGCTCATAC TCCAGTTTTA AGTCCTTCCC TTCCTGATAT GTCTCTATCT
    ACACATTCGT CTCTTCAGTC TGGTGGATGG CGAAAACTCC CACCTAACCT CAGTCCCACC
    ATAGAGTATA ATGATGGAAG ACCAGTAAAG CGCCATGATA TAGCACGCTC TCATTCCGAA
    AGTCCTTCCA GACTTCCCAT CAATAGGTCA GGGACCTGGA AACGTGAGCA CAGCAAACAC
    TCATCATCAC TTCCTCGAGT AAGCACTTGG AGAAGAACTG GAAGTTCATC CTCAATTCTT
    TCTGCTTCAT CAGAATCTAG TGAAAAGGCA AAAAGTGAGG ACGAAAAACA AGTGAACTCT
    ATTTCAGGAA GCAAACAAAC TAAAGAAAAC CAGGTATCCA CAAAAGGAAC ATGGAGAAAA
    ATAAAAGAAA GTGAAATTTC TCCCACAAAT AGTACTTCTC AGACCACTTC TTCAGGTGCT
    GCAAATGGTG CTGAATCAAA GACTCTGATT TATCAAATGG CACCTGCTGT TTCTAAAACA
    GAGGATGTTT GGGTGAGAAT TGAGGATTGC CCCATTAACA ACCCTAGATC TGGAAGATCT
    CCAACAGGAA ATACTCCCCC CGTGATTGAC ACTGTTTCAG AAAAGGGAAA CCCGAACCCT
    AAAGATTCAA AAGATAATCA GGGAAAACAA AATGTGAGCA ATGGTAGTGC CCCTACACGC
    ACCATGGGTC TGGAAAACCG CCTGAATTCC TTTATTCAGG TAGATCCTCC AGACCAAAAA
    GGAACTGAGA CAAAACCGGG ACACAGTAAT AACCCTGTCC CTGCATCCGA GACTAGTGAA
    AGTTCTATAG CCGAGCGTAC CCCGTTTAGT TCTAGCAGCT CAAGCAAGCA CAGTTCACCA
    AGTGGGACTG TCGCCGCCAG GGTGAGTCCT TTTAATTACA ACCCAAGCCC AAGGAAGAGC
    AGCACAGATG GCACTTCAGC CCGACCGTCT CAGATCCCAA CGCCAGTGAG CAACAACACA
    AAGAAACGCG ACTCAAAACC CGACAGCACA GAACCCAGCG GGACTCAAAG TCCTAAACGC
    CATTCTGGGT CTTACCTTGT GACATCTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211260.1
    CDS329..8581
    Translation

    Target ORF information:

    RefSeq Version XM_005211203.4
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211203.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGATACGAG CTTACTGTGA AACCTGTTGG
    GAGTGGCAGG AAGCCCATGA ACAAGGGATG GACCAGGACA AAAATCCAAT GCCAGCTCCT
    GTTGAACATC AGATCTGCCC TGCTGTCTGT GTTCTAATGA AGCTTTCGTT TGATGAAGAG
    CATAGACATG CCATGAATGA GCTTGGGGGA CTACAGGCCA TTGCAGAATT ATTGCAAGTG
    GACTGTGAAA TGTATGGGCT TACTAATGAC CACTACAGTA TTACTTTAAG ACGATATGCA
    GGAATGGCTT TGACAAACTT GACTTTCGGA GATGTAGCCA ACAAGGCTAC TCTCTGCTCT
    ATGAAAGGCT GCATGAGAGC GCTCGTGGCT CAGCTCCAGT CTGAAAGCGA GGACTTGCAG
    CAGGTTATTG CGAGTGTTTT GAGGAATCTG TCTTGGAGAG CAGATGTAAA TAGTAAAAAG
    ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTGAAAAAG
    GAATCAACCC TCAAAAGTGT ATTGAGTGCC TTATGGAACT TGTCAGCACA CTGCACTGAG
    AATAAAGCTG ACATATGTGC TGTGGATGGT GCGCTTGCAT TTTTGGTTGG CACTCTCACT
    TATCGCAGCC AGACCAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGTAT ATTACGGAAT
    GTATCCAGCT TGATAGCTAC AAATGAGGAC CACAGGCAAA TCCTAAGAGA GAATAATTGC
    TTACAGACCT TATTACAACA CTTGAAATCT CACAGTTTGA CAATAGTCAG CAATGCATGC
    GGAACCTTGT GGAATCTCTC AGCAAGAAAC CCTAAAGACC AGGAAGCATT GTGGGACATG
    GGAGCAGTCA GCATGCTCAA GAACCTCATT CATTCAAAGC ACAAGATGAT TGCTATGGGA
    AGTGCTGCAG CTTTAAGGAA TCTCATGGCA AATAGACCTG CAAAGTATAA AGATGCCAAT
    ATCATGTCTC CTGGTTCAAG TTTGCCTTCT CTACATGTCA GGAAACAAAA GGCCCTGGAA
    GCAGAATTAG ATGCTCAGCA TTTATCAGAA ACTTTTGACA ATATTGACAA TTTAAGTCCC
    AAGGCATCTC ATCGTAGCAA GCAGAGACAC AAGCAAAATC TCTATGGTGA CTATGTTTTT
    GACACCAATC GACATGATGA TAACAGGTCA GACAATTTTA ATACTGGAAA CATGACTGTC
    CTATCACCGT ACTTAAACAC TACAGTATTG CCCAGCTCTT CTTCATCAAG GGGAAGTTTA
    GATAGCTCTC GTTCTGAGAA AGATAGAAGT CTGGAGAGAG AACGAGGTAT TAGCCTAGGC
    AACTATCACC CAGCAACAGA AAACCCAGGA ACCTCTTCAA AGCGAGGTTT GCAGATTTCT
    ACCACTGCAG CCCAGATTGC CAAAGTCATG GAAGAAGTAT CAGCTATTCA TACCTCTCAG
    GAAGACAGAA GTTCTGGGTC TACCACAGAA CTACACTGTG GGACAGATGA GAGGAATGCA
    CTAAGAAGAA GCTCTACCAC CCACACACAT GCAAACACGT ACAACTTTAC CAAGTCAGAA
    AACTCAAACA GAACATGTCC AATACCATAT GCCAAAGTAG AATATAAGAG ATCTTCAAAT
    GATAGTTTAA ATAGTGTCAG CAGTAGTGAT GGTTATGGTA AAAGAGGTCA GATGAAACCT
    TCAATTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGCTATGG TCAGTATCCA
    GCTGACCTAG CCCATAAAAT ACATAGTGCA AATCATATGG ATGATAATGA TGGAGAACTA
    GATACACCAA TAAATTACAG TCTCAAATAT TCCGATGAAC AGTTGAACTC TGGGAGGCAG
    AGCCCTTCAC AGAATGAAAG ATGGGCAAGA CCCAAACATA TACTAGAAGA TGAAATAAAA
    CCGAATGAGC AGAGACAATC AAGGAGTCAA AGCACAGCTT ATCCCGTGTA TCCTGAGAGC
    ACTGATGATA AACACCTCAA GTTCCAACCA CACTTTGGGC AGCAAGAATG TGTTTCCCCA
    TACAGGTCAA GAGCAGCCAA TGGATCTGAA ACAAATCGAG TAGGCTCTAA TCATGGAATT
    AGTCAAAATG TGAACCAGTC TTTGTGTCAA GAAGATGACT ATGAAGACGA TAAACCAACC
    AACTATAGCG AACGTTACTC TGAGGAAGGG CAGCATGAGG AAGAAGAGAG GCCAACCAAT
    TATAGCATAA AATACAGTGA AGAAAAACAT CACGTGGATC AGCCTATTGA TTATAGTTTA
    AAATACACCA CAGACATTCC TTCTTCACAG AAACCAGCAT TTTCATTCTC AAAGAATTCA
    TCTGGACAGA GCACAAAAAC TGAACACATC TCTTCAAGCA GCGAGAATAC ATCCACAACT
    TCATCTAATG CCAAGAGGCA GAATCAGCTG CATCCAAGCT CAGCACAGAG CAGAAGTGGT
    CAGACCCCAA AAGCCACCTC TTCCTCTTGC AAAGTCCCCT CTATCAACCA AGAAACAATA
    CAGACTTACT GTGTAGAAGA TACCCCAATA TGTTTTTCAA GATGCAGTTC ATTATCATCT
    CTGTCATCTG CTGAAGATGA AGTAGGGTGT GATCAGACAA CACAAGAAGC AGAGTCTGCT
    AACACTCTGC AAATAGCTGA AATCAAGGAC AACAGCGGAC CTAGGTCAAA TGAAGATTCT
    GTGAGTAAAG TTCCAGCAGG GTCACAGCAC ATTAGAACCA AATCCAGCAG ACTCCAGGCT
    TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC AAAGCTGTTG AATTTTCTTC AGGGGCCAAA
    TCTCCTTCCA AGAGCGGTGC TCAGACACCC AAAAGTCCAC CAGAGCACTA CGTTCAGGAG
    ACCCCACTCA TGTTTAGCAG ATGTACTTCA GTCAGTTCAC TCGACAGTTT TGAGAGTCGC
    TCGATTGCCA GCTCTGTTCA GAGTGAACCC TGCAGTGGAA TGGTAAGTGG CATCATAAGC
    CCCAGTGACC TCCCAGATAG CCCTGGACAA ACCATGCCAC CAAGCAGAAG CAAAACCCCT
    CCGCCACCGC CTCCGCCTCC TCCTCAGACA GTTCAAACGA AGCAGGAAGT ACCTAAAAAT
    AAAGCACCTA GTGCTGAAAA GAGAGAAAGT GGACCCAAGC AAGCTGCTGT AAATGCTGCA
    GTACAAAGGG TCCAGGTTCT TCCAGAGGCT GATACTCTGT TACATTTTGC CACAGAGAGT
    ACTCCTGATG GATTTTCTTG TTCATCTAGC CTGAGCGCTC TGAGCCTTGA TGAGCCATTT
    ATTCAGAAAG ATGTGGAACT AAGAATAATG CCTCCAGTTC AGGAAAATGA CAACGGGAAT
    GAAACAGAAA GTGAGCAGCC TGAAGAATCA AATGAAAACC AGGAAAAAGA GGCAGAAAAA
    CCCACTGATT CTGAAAAAGA TCTTTTAGAT GAGTCAGATG ATGATGATAT TGAAATACTA
    GAAGAGTGTA TAATTTCTGC CATGCCAACA AAATCTTCAC GCAAAGCCAA AAAACCAGCC
    CAGACTACTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT CCCTGTATAC
    AAACTTCTGC CATCGCAAAA CAGGTTACAG GCACAAAAGC ATGTTAGTTT TACACCAGGA
    GATGATATGC CACGGGTGTA TTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCTACA
    TCTCTGAGTG ATCTAACGAT AGAATCCCCT CCAAATGAGT TAGCTGCTGG AGAAGGGGTT
    AGAGCAGGGG CACAGTCAAG TGAGTTTGAA AAACGAGATA CCATTCCTAC TGAAGGCAGA
    AGTACAGATG AGGCTCAACG AGGGAAAGCC TCATCTGTCA CTGTGCCTGA ACTGGACGAC
    AGCAAAACAG AAGAAGGTGA TATTCTTGCA GAATGCATTA ATTCTGCCAT GCCCAAAGGA
    AAAAGTCACA AGCCTTTCCG TGTGAAAAAG ATCATGGACC AGGTCCAGCA AGCATCTATG
    TCTTCATCTG GAACTAACAA AAATCAATTA GATGGTAAGA CGAAGAAACC TACTTCACCA
    GTAAAACCTA TACCACAAAA TACTGAATAC AGGACACGTG TAAGAAAAAA TACAGACTCA
    AAAAATAATC TAAATGCAGA AAGAAATTTC TCGGAAAACA AAGACTCAAA GAAACAGCAC
    TTGAAAAATA ATTCCAAGGA CTTCAATGAT AAACTGCCAA ATAATGAAGA CAGAGTCAGA
    GGAAGTTTTA CTTTTGATTC ACCCCATCAT TACACGCCCA TTGAAGGCAC TCCGTACTGT
    TTTTCACGAA ATGACTCTCT GAGTTCTCTA GATTTTGATG ATGATGATGT TGACCTTTCC
    AGGGAAAAGG CTGAATTAAG AAAGGGGAAG GAAAATAAAG AATCAGAAGC TAAAGTGACC
    AACCACACAG AACTAACCTC AAACCAACAA TCAGCTAGTA AGACACCAGC TGTTACAAAG
    CAGCCAATAA ATAGAGGTCA GTCTAAACCC GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG
    TCTTCCAAAG ATATACCAGA CAGAGGGGCA GCAACAGATG AGAAATTACA GAATTTCGCT
    ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TAAGCTCTCT TAGTGACATT
    GATCAAGAAA ACAACAACAA CAAGGAAAAT GAACCTGTCA AAGAGACAGA GCCCCCTGCC
    TCACAGGGAG AACCAGGTAA ACCCCAGGCC TCAGGTTATG CTCCTAAATC GTTTCACGTG
    GAAGACACCC CTGTTTGTTT CTCAAGAAAC AGTTCTCTCA GTTCTCTTAG TATTGATTCT
    GAAGATGACC TGCTGCAGGA ATGTATAAGT TCTGCAATGC CAAAAAAGAA AAAGCCTTCA
    AGGCTCAAGC CCGATAATGA AAAGCATAGT CCCAGAAATA TGGGTGGCAT ATTAGCAGAA
    GATTTGACAC TCGATTTGAA AGATATACAG AGACCAGATT CAGAACATGG TTTATCCCCA
    GATTCAGAAA ATTTTGATTG GAAAGCTATT CAGGAAGGTG CAAATTCCAT AGTAAGTAGT
    TTACATCAAG CTGCTGCCGC TGCATGTTTA TCTCGACAAG CTTCATCTGA TTCAGATTCT
    ATCCTTTCAT TGAAATCGGG TATCTCTCTG GGATCACCAT TTCACCTTAC ACCTGATCAA
    GAAGAAAAAC CCTTTACGAG TAATAAAGGT CCACGAATTC TAAAACCTGG GGAGAAAAGT
    ACATTGGAAA CTAAAAAAAT AGAATCTGAA AATAAAGGAA TAAAAGGAGG CAAAAAGGTT
    TATAAAAGTT TGATTACTGG AAAAGTTCGA TCTAATTCGG AAATTTCGAG CCAAATGAAA
    CAACCCCTTC AAACAAACAT GCCTTCAATC TCTCGAGGTA GGACAATGAT TCATATTCCA
    GGAGTTCGGA ATAGCTCTTC AAGTACAAGC CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG
    ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT CAGCCGGCTA CCACCTCTCC CAGAGGAACC
    AAGCCATCAG TGAAGTCAGA ATTAAGCCCT GTTACAAGGC AGGCATCCCA GACAGCAGGA
    TCAAACAAAG GACCTTCTAG ATCAGGATCT AGAGATTCCA CTCCTTCAAG ACCTGCCCAG
    CAACCATTAA GTAGACCAAT GCAGTCTCCA GGGCGAAACT CAATCTCTCC TGGTAGAAAT
    GGAATAAGTC CCCCTAACAA ATTATCTCAA CTACCAAGGA CGTCATCCCC TAGTACTGCT
    TCAACTAAGT CCTCAGGTTC TGGGAAAATG TCTTACACAT CTCCTGGCAG ACAGATGAGC
    CAGCAGAACC TCACCAAACA AACAGGCTTA TCCAAGAATG GCAGTGGTAT CCCAAGAAGT
    GAATCTGCCT CCAAAGGGCT AAATCAAATG AGTAATAGTA ATGGATCCAA TAAAAAAGTA
    GAACTTTCTA GAATGTCTTC AACAAAGTCA AGTGGAAGTG AATCCGATAG GTCAGAGAGA
    CCTGTATTAG TACGCCAATC AACTTTCATC AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA
    AAACTGGAGG AATCTGCTTC ATTTGAATCT CTTTCTCCAT CTTCCAGACC CGATTCTCCC
    ACACGGTCCC AGGCTCATAC TCCAGTTTTA AGTCCTTCCC TTCCTGATAT GTCTCTATCT
    ACACATTCGT CTCTTCAGTC TGGTGGATGG CGAAAACTCC CACCTAACCT CAGTCCCACC
    ATAGAGTATA ATGATGGAAG ACCAGTAAAG CGCCATGATA TAGCACGCTC TCATTCCGAA
    AGTCCTTCCA GACTTCCCAT CAATAGGTCA GGGACCTGGA AACGTGAGCA CAGCAAACAC
    TCATCATCAC TTCCTCGAGT AAGCACTTGG AGAAGAACTG GAAGTTCATC CTCAATTCTT
    TCTGCTTCAT CAGAATCTAG TGAAAAGGCA AAAAGTGAGG ACGAAAAACA AGTGAACTCT
    ATTTCAGGAA GCAAACAAAC TAAAGAAAAC CAGGTATCCA CAAAAGGAAC ATGGAGAAAA
    ATAAAAGAAA GTGAAATTTC TCCCACAAAT AGTACTTCTC AGACCACTTC TTCAGGTGCT
    GCAAATGGTG CTGAATCAAA GACTCTGATT TATCAAATGG CACCTGCTGT TTCTAAAACA
    GAGGATGTTT GGGTGAGAAT TGAGGATTGC CCCATTAACA ACCCTAGATC TGGAAGATCT
    CCAACAGGAA ATACTCCCCC CGTGATTGAC ACTGTTTCAG AAAAGGGAAA CCCGAACCCT
    AAAGATTCAA AAGATAATCA GGGAAAACAA AATGTGAGCA ATGGTAGTGC CCCTACACGC
    ACCATGGGTC TGGAAAACCG CCTGAATTCC TTTATTCAGG TAGATCCTCC AGACCAAAAA
    GGAACTGAGA CAAAACCGGG ACACAGTAAT AACCCTGTCC CTGCATCCGA GACTAGTGAA
    AGTTCTATAG CCGAGCGTAC CCCGTTTAGT TCTAGCAGCT CAAGCAAGCA CAGTTCACCA
    AGTGGGACTG TCGCCGCCAG GGTGAGTCCT TTTAATTACA ACCCAAGCCC AAGGAAGAGC
    AGCACAGATG GCACTTCAGC CCGACCGTCT CAGATCCCAA CGCCAGTGAG CAACAACACA
    AAGAAACGCG ACTCAAAACC CGACAGCACA GAACCCAGCG GGACTCAAAG TCCTAAACGC
    CATTCTGGGT CTTACCTTGT GACATCTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219622
    Clone ID Related Accession (Same CDS sequence) XM_015472988.2
    Accession Version XM_015472988.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
    2221
    2281
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    2581
    2641
    2701
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    2941
    3001
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    3121
    3181
    3241
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    4141
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    4261
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    4441
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    4681
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    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGACCAGAA GGCAGTTGGA ATATGAGGCA AGGCAAATCA GAGTTGCAAT GGAAGAACAA 
    CTAGGTACTT GCCAGGATAT GGAAAAACGA GCACAGCGAA GAATAACCAG AATTCAACAA
    ATAGAAAAGG ACATACTTCG TATACGACAG CTTTTACAGT CCCAAGCAAC AGAAGCAGAG
    AGGTCATCTC AGAGCAAGCA TGAAGCGGGC TCACATGAAG CTGAACGGCA GAATGAAGGT
    CAAGGAGTGG CAGAAATCAA CATGGCAACC TCGGGTAGCG GTCAGGTTAC TGCTTCTCTG
    GTCCCTCTGA AGGATGTCGG TGCAGACTTA ATCATACTAA AAAGGCCCAG GAAAGATTCT
    CAAGACTCGG GTTCTGGCTT ACAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCACCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328474.1
    CDS213..8372
    Translation

    Target ORF information:

    RefSeq Version XM_015472988.2
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472988.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGACCAGAA GGCAGTTGGA ATATGAGGCA AGGCAAATCA GAGTTGCAAT GGAAGAACAA 
    CTAGGTACTT GCCAGGATAT GGAAAAACGA GCACAGCGAA GAATAACCAG AATTCAACAA
    ATAGAAAAGG ACATACTTCG TATACGACAG CTTTTACAGT CCCAAGCAAC AGAAGCAGAG
    AGGTCATCTC AGAGCAAGCA TGAAGCGGGC TCACATGAAG CTGAACGGCA GAATGAAGGT
    CAAGGAGTGG CAGAAATCAA CATGGCAACC TCGGGTAGCG GTCAGGTTAC TGCTTCTCTG
    GTCCCTCTGA AGGATGTCGG TGCAGACTTA ATCATACTAA AAAGGCCCAG GAAAGATTCT
    CAAGACTCGG GTTCTGGCTT ACAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCACCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219623
    Clone ID Related Accession (Same CDS sequence) XM_024997643.1
    Accession Version XM_024997643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGTAGGGT ATCACTCCAG GACCTTGTTT CAGCGAAGAA TAACCAGAAT TCAACAAATA 
    GAAAAGGACA TACTTCGTAT ACGACAGCTT TTACAGTCCC AAGCAACAGA AGCAGAGAGG
    TCATCTCAGA GCAAGCATGA AGCGGGCTCA CATGAAGCTG AACGGCAGAA TGAAGGTCAA
    GGAGTGGCAG AAATCAACAT GGCAACCTCG GGTAGCGGTC AGGTTACTGC TTCTCTGGTC
    CCTCTGAAGG ATGTCGGTGC AGACTTAATC ATACTAAAAA GGCCCAGGAA AGATTCTCAA
    GACTCGGGTT CTGGCTTACA GGGTTCAACT ACACGAATAG ATCATGAAAC AGCCAGTGTT
    TTGAGTTCTA GTAGCACACA TTCTGCTCCT CGAAGGCTGA CGAGTCATCT GGGAACCAAG
    GTGGAAATGG TGTATTCATT GTTGTCAATG CTTGGTACTC ATGATAAGGA TGATATGTCG
    CGAACTTTGC TAGCTATGTC TAGCTCCCAA GACAGCTGTA TATCCATGCG ACAGTCTGGA
    TGTCTTCCTC TCCTCATCCA GCTTTTACAT GGCAATGACA AAGACTCTGT GTTGTTGGGA
    AATTCCCGGG GCAGTAAAGA GGCTCGGGCC AGGGCCAGTG CAGCACTCCA CAACATCATT
    CACTCACAGC CTGATGACAA GAGAGGCAGG CGTGAAATCC GAGTCCTTCA TCTTTTGGAA
    CAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGTAGG
    AAGGCTACCC GGGGCATTTC ATCCCAGGAG CTAGGGCAGG GGCTTTCAGG GGGACTACAG
    GCCATTGCAG AATTATTGCA AGTGGACTGT GAAATGTATG GGCTTACTAA TGACCACTAC
    AGTATTACTT TAAGACGATA TGCAGGAATG GCTTTGACAA ACTTGACTTT CGGAGATGTA
    GCCAACAAGG CTACTCTCTG CTCTATGAAA GGCTGCATGA GAGCGCTCGT GGCTCAGCTC
    CAGTCTGAAA GCGAGGACTT GCAGCAGGTT ATTGCGAGTG TTTTGAGGAA TCTGTCTTGG
    AGAGCAGATG TAAATAGTAA AAAGACTTTG CGTGAAGTTG GAAGTGTGAA AGCATTGATG
    GAATGTGCTT TGGAAGTGAA AAAGGAATCA ACCCTCAAAA GTGTATTGAG TGCCTTATGG
    AACTTGTCAG CACACTGCAC TGAGAATAAA GCTGACATAT GTGCTGTGGA TGGTGCGCTT
    GCATTTTTGG TTGGCACTCT CACTTATCGC AGCCAGACCA ATACTTTAGC CATTATTGAA
    AGTGGAGGTG GTATATTACG GAATGTATCC AGCTTGATAG CTACAAATGA GGACCACAGG
    CAAATCCTAA GAGAGAATAA TTGCTTACAG ACCTTATTAC AACACTTGAA ATCTCACAGT
    TTGACAATAG TCAGCAATGC ATGCGGAACC TTGTGGAATC TCTCAGCAAG AAACCCTAAA
    GACCAGGAAG CATTGTGGGA CATGGGAGCA GTCAGCATGC TCAAGAACCT CATTCATTCA
    AAGCACAAGA TGATTGCTAT GGGAAGTGCT GCAGCTTTAA GGAATCTCAT GGCAAATAGA
    CCTGCAAAGT ATAAAGATGC CAATATCATG TCTCCTGGTT CAAGTTTGCC TTCTCTACAT
    GTCAGGAAAC AAAAGGCCCT GGAAGCAGAA TTAGATGCTC AGCATTTATC AGAAACTTTT
    GACAATATTG ACAATTTAAG TCCCAAGGCA TCTCATCGTA GCAAGCAGAG ACACAAGCAA
    AATCTCTATG GTGACTATGT TTTTGACACC AATCGACATG ATGATAACAG GTCAGACAAT
    TTTAATACTG GAAACATGAC TGTCCTATCA CCGTACTTAA ACACTACAGT ATTGCCCAGC
    TCTTCTTCAT CAAGGGGAAG TTTAGATAGC TCTCGTTCTG AGAAAGATAG AAGTCTGGAG
    AGAGAACGAG GTATTAGCCT AGGCAACTAT CACCCAGCAA CAGAAAACCC AGGAACCTCT
    TCAAAGCGAG GTTTGCAGAT TTCTACCACT GCAGCCCAGA TTGCCAAAGT CATGGAAGAA
    GTATCAGCTA TTCATACCTC TCAGGAAGAC AGAAGTTCTG GGTCTACCAC AGAACTACAC
    TGTGGGACAG ATGAGAGGAA TGCACTAAGA AGAAGCTCTA CCACCCACAC ACATGCAAAC
    ACGTACAACT TTACCAAGTC AGAAAACTCA AACAGAACAT GTCCAATACC ATATGCCAAA
    GTAGAATATA AGAGATCTTC AAATGATAGT TTAAATAGTG TCAGCAGTAG TGATGGTTAT
    GGTAAAAGAG GTCAGATGAA ACCTTCAATT GAATCCTATT CTGAAGATGA TGAAAGTAAA
    TTTTGCAGCT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AAATACATAG TGCAAATCAT
    ATGGATGATA ATGATGGAGA ACTAGATACA CCAATAAATT ACAGTCTCAA ATATTCCGAT
    GAACAGTTGA ACTCTGGGAG GCAGAGCCCT TCACAGAATG AAAGATGGGC AAGACCCAAA
    CATATACTAG AAGATGAAAT AAAACCGAAT GAGCAGAGAC AATCAAGGAG TCAAAGCACA
    GCTTATCCCG TGTATCCTGA GAGCACTGAT GATAAACACC TCAAGTTCCA ACCACACTTT
    GGGCAGCAAG AATGTGTTTC CCCATACAGG TCAAGAGCAG CCAATGGATC TGAAACAAAT
    CGAGTAGGCT CTAATCATGG AATTAGTCAA AATGTGAACC AGTCTTTGTG TCAAGAAGAT
    GACTATGAAG ACGATAAACC AACCAACTAT AGCGAACGTT ACTCTGAGGA AGGGCAGCAT
    GAGGAAGAAG AGAGGCCAAC CAATTATAGC ATAAAATACA GTGAAGAAAA ACATCACGTG
    GATCAGCCTA TTGATTATAG TTTAAAATAC ACCACAGACA TTCCTTCTTC ACAGAAACCA
    GCATTTTCAT TCTCAAAGAA TTCATCTGGA CAGAGCACAA AAACTGAACA CATCTCTTCA
    AGCAGCGAGA ATACATCCAC AACTTCATCT AATGCCAAGA GGCAGAATCA GCTGCATCCA
    AGCTCAGCAC AGAGCAGAAG TGGTCAGACC CCAAAAGCCA CCTCTTCCTC TTGCAAAGTC
    CCCTCTATCA ACCAAGAAAC AATACAGACT TACTGTGTAG AAGATACCCC AATATGTTTT
    TCAAGATGCA GTTCATTATC ATCTCTGTCA TCTGCTGAAG ATGAAGTAGG GTGTGATCAG
    ACAACACAAG AAGCAGAGTC TGCTAACACT CTGCAAATAG CTGAAATCAA GGACAACAGC
    GGACCTAGGT CAAATGAAGA TTCTGTGAGT AAAGTTCCAG CAGGGTCACA GCACATTAGA
    ACCAAATCCA GCAGACTCCA GGCTTCTGGT CTGTCTTCAG AGTCAGCCAG GCACAAAGCT
    GTTGAATTTT CTTCAGGGGC CAAATCTCCT TCCAAGAGCG GTGCTCAGAC ACCCAAAAGT
    CCACCAGAGC ACTACGTTCA GGAGACCCCA CTCATGTTTA GCAGATGTAC TTCAGTCAGT
    TCACTCGACA GTTTTGAGAG TCGCTCGATT GCCAGCTCTG TTCAGAGTGA ACCCTGCAGT
    GGAATGGTAA GTGGCATCAT AAGCCCCAGT GACCTCCCAG ATAGCCCTGG ACAAACCATG
    CCACCAAGCA GAAGCAAAAC CCCTCCGCCA CCGCCTCCGC CTCCTCCTCA GACAGTTCAA
    ACGAAGCAGG AAGTACCTAA AAATAAAGCA CCTAGTGCTG AAAAGAGAGA AAGTGGACCC
    AAGCAAGCTG CTGTAAATGC TGCAGTACAA AGGGTCCAGG TTCTTCCAGA GGCTGATACT
    CTGTTACATT TTGCCACAGA GAGTACTCCT GATGGATTTT CTTGTTCATC TAGCCTGAGC
    GCTCTGAGCC TTGATGAGCC ATTTATTCAG AAAGATGTGG AACTAAGAAT AATGCCTCCA
    GTTCAGGAAA ATGACAACGG GAATGAAACA GAAAGTGAGC AGCCTGAAGA ATCAAATGAA
    AACCAGGAAA AAGAGGCAGA AAAACCCACT GATTCTGAAA AAGATCTTTT AGATGAGTCA
    GATGATGATG ATATTGAAAT ACTAGAAGAG TGTATAATTT CTGCCATGCC AACAAAATCT
    TCACGCAAAG CCAAAAAACC AGCCCAGACT ACTTCAAAAT TACCTCCACC TGTGGCAAGG
    AAACCAAGTC AGCTCCCTGT ATACAAACTT CTGCCATCGC AAAACAGGTT ACAGGCACAA
    AAGCATGTTA GTTTTACACC AGGAGATGAT ATGCCACGGG TGTATTGTGT AGAAGGGACA
    CCTATAAACT TTTCCACAGC TACATCTCTG AGTGATCTAA CGATAGAATC CCCTCCAAAT
    GAGTTAGCTG CTGGAGAAGG GGTTAGAGCA GGGGCACAGT CAAGTGAGTT TGAAAAACGA
    GATACCATTC CTACTGAAGG CAGAAGTACA GATGAGGCTC AACGAGGGAA AGCCTCATCT
    GTCACTGTGC CTGAACTGGA CGACAGCAAA ACAGAAGAAG GTGATATTCT TGCAGAATGC
    ATTAATTCTG CCATGCCCAA AGGAAAAAGT CACAAGCCTT TCCGTGTGAA AAAGATCATG
    GACCAGGTCC AGCAAGCATC TATGTCTTCA TCTGGAACTA ACAAAAATCA ATTAGATGGT
    AAGACGAAGA AACCTACTTC ACCAGTAAAA CCTATACCAC AAAATACTGA ATACAGGACA
    CGTGTAAGAA AAAATACAGA CTCAAAAAAT AATCTAAATG CAGAAAGAAA TTTCTCGGAA
    AACAAAGACT CAAAGAAACA GCACTTGAAA AATAATTCCA AGGACTTCAA TGATAAACTG
    CCAAATAATG AAGACAGAGT CAGAGGAAGT TTTACTTTTG ATTCACCCCA TCATTACACG
    CCCATTGAAG GCACTCCGTA CTGTTTTTCA CGAAATGACT CTCTGAGTTC TCTAGATTTT
    GATGATGATG ATGTTGACCT TTCCAGGGAA AAGGCTGAAT TAAGAAAGGG GAAGGAAAAT
    AAAGAATCAG AAGCTAAAGT GACCAACCAC ACAGAACTAA CCTCAAACCA ACAATCAGCT
    AGTAAGACAC CAGCTGTTAC AAAGCAGCCA ATAAATAGAG GTCAGTCTAA ACCCGTGCTG
    CAGAAGCAGT CCACTTTTCC CCAGTCTTCC AAAGATATAC CAGACAGAGG GGCAGCAACA
    GATGAGAAAT TACAGAATTT CGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC
    TCTCTAAGCT CTCTTAGTGA CATTGATCAA GAAAACAACA ACAACAAGGA AAATGAACCT
    GTCAAAGAGA CAGAGCCCCC TGCCTCACAG GGAGAACCAG GTAAACCCCA GGCCTCAGGT
    TATGCTCCTA AATCGTTTCA CGTGGAAGAC ACCCCTGTTT GTTTCTCAAG AAACAGTTCT
    CTCAGTTCTC TTAGTATTGA TTCTGAAGAT GACCTGCTGC AGGAATGTAT AAGTTCTGCA
    ATGCCAAAAA AGAAAAAGCC TTCAAGGCTC AAGCCCGATA ATGAAAAGCA TAGTCCCAGA
    AATATGGGTG GCATATTAGC AGAAGATTTG ACACTCGATT TGAAAGATAT ACAGAGACCA
    GATTCAGAAC ATGGTTTATC CCCAGATTCA GAAAATTTTG ATTGGAAAGC TATTCAGGAA
    GGTGCAAATT CCATAGTAAG TAGTTTACAT CAAGCTGCTG CCGCTGCATG TTTATCTCGA
    CAAGCTTCAT CTGATTCAGA TTCTATCCTT TCATTGAAAT CGGGTATCTC TCTGGGATCA
    CCATTTCACC TTACACCTGA TCAAGAAGAA AAACCCTTTA CGAGTAATAA AGGTCCACGA
    ATTCTAAAAC CTGGGGAGAA AAGTACATTG GAAACTAAAA AAATAGAATC TGAAAATAAA
    GGAATAAAAG GAGGCAAAAA GGTTTATAAA AGTTTGATTA CTGGAAAAGT TCGATCTAAT
    TCGGAAATTT CGAGCCAAAT GAAACAACCC CTTCAAACAA ACATGCCTTC AATCTCTCGA
    GGTAGGACAA TGATTCATAT TCCAGGAGTT CGGAATAGCT CTTCAAGTAC AAGCCCAGTT
    TCTAAAAAAG GCCCGCCCCT CAAGACTCCA GCCTCCAAAA GCCCTAGTGA AGGTCAGCCG
    GCTACCACCT CTCCCAGAGG AACCAAGCCA TCAGTGAAGT CAGAATTAAG CCCTGTTACA
    AGGCAGGCAT CCCAGACAGC AGGATCAAAC AAAGGACCTT CTAGATCAGG ATCTAGAGAT
    TCCACTCCTT CAAGACCTGC CCAGCAACCA TTAAGTAGAC CAATGCAGTC TCCAGGGCGA
    AACTCAATCT CTCCTGGTAG AAATGGAATA AGTCCCCCTA ACAAATTATC TCAACTACCA
    AGGACGTCAT CCCCTAGTAC TGCTTCAACT AAGTCCTCAG GTTCTGGGAA AATGTCTTAC
    ACATCTCCTG GCAGACAGAT GAGCCAGCAG AACCTCACCA AACAAACAGG CTTATCCAAG
    AATGGCAGTG GTATCCCAAG AAGTGAATCT GCCTCCAAAG GGCTAAATCA AATGAGTAAT
    AGTAATGGAT CCAATAAAAA AGTAGAACTT TCTAGAATGT CTTCAACAAA GTCAAGTGGA
    AGTGAATCCG ATAGGTCAGA GAGACCTGTA TTAGTACGCC AATCAACTTT CATCAAAGAA
    GCTCCAAGCC CAACCCTAAG GAGAAAACTG GAGGAATCTG CTTCATTTGA ATCTCTTTCT
    CCATCTTCCA GACCCGATTC TCCCACACGG TCCCAGGCTC ATACTCCAGT TTTAAGTCCT
    TCCCTTCCTG ATATGTCTCT ATCTACACAT TCGTCTCTTC AGTCTGGTGG ATGGCGAAAA
    CTCCCACCTA ACCTCAGTCC CACCATAGAG TATAATGATG GAAGACCAGT AAAGCGCCAT
    GATATAGCAC GCTCTCATTC CGAAAGTCCT TCCAGACTTC CCATCAATAG GTCAGGGACC
    TGGAAACGTG AGCACAGCAA ACACTCATCA TCACTTCCTC GAGTAAGCAC TTGGAGAAGA
    ACTGGAAGTT CATCCTCAAT TCTTTCTGCT TCATCAGAAT CTAGTGAAAA GGCAAAAAGT
    GAGGACGAAA AACAAGTGAA CTCTATTTCA GGAAGCAAAC AAACTAAAGA AAACCAGGTA
    TCCACAAAAG GAACATGGAG AAAAATAAAA GAAAGTGAAA TTTCTCCCAC AAATAGTACT
    TCTCAGACCA CTTCTTCAGG TGCTGCAAAT GGTGCTGAAT CAAAGACTCT GATTTATCAA
    ATGGCACCTG CTGTTTCTAA AACAGAGGAT GTTTGGGTGA GAATTGAGGA TTGCCCCATT
    AACAACCCTA GATCTGGAAG ATCTCCAACA GGAAATACTC CCCCCGTGAT TGACACTGTT
    TCAGAAAAGG GAAACCCGAA CCCTAAAGAT TCAAAAGATA ATCAGGGAAA ACAAAATGTG
    AGCAATGGTA GTGCCCCTAC ACGCACCATG GGTCTGGAAA ACCGCCTGAA TTCCTTTATT
    CAGGTAGATC CTCCAGACCA AAAAGGAACT GAGACAAAAC CGGGACACAG TAATAACCCT
    GTCCCTGCAT CCGAGACTAG TGAAAGTTCT ATAGCCGAGC GTACCCCGTT TAGTTCTAGC
    AGCTCAAGCA AGCACAGTTC ACCAAGTGGG ACTGTCGCCG CCAGGGTGAG TCCTTTTAAT
    TACAACCCAA GCCCAAGGAA GAGCAGCACA GATGGCACTT CAGCCCGACC GTCTCAGATC
    CCAACGCCAG TGAGCAACAA CACAAAGAAA CGCGACTCAA AACCCGACAG CACAGAACCC
    AGCGGGACTC AAAGTCCTAA ACGCCATTCT GGGTCTTACC TTGTGACATC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853411.1
    CDS1801..9897
    Translation

    Target ORF information:

    RefSeq Version XM_024997643.1
    Organism Bos taurus(cattle)
    Definition Bos taurus APC, WNT signaling pathway regulator (APC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGTAGGGT ATCACTCCAG GACCTTGTTT CAGCGAAGAA TAACCAGAAT TCAACAAATA 
    GAAAAGGACA TACTTCGTAT ACGACAGCTT TTACAGTCCC AAGCAACAGA AGCAGAGAGG
    TCATCTCAGA GCAAGCATGA AGCGGGCTCA CATGAAGCTG AACGGCAGAA TGAAGGTCAA
    GGAGTGGCAG AAATCAACAT GGCAACCTCG GGTAGCGGTC AGGTTACTGC TTCTCTGGTC
    CCTCTGAAGG ATGTCGGTGC AGACTTAATC ATACTAAAAA GGCCCAGGAA AGATTCTCAA
    GACTCGGGTT CTGGCTTACA GGGTTCAACT ACACGAATAG ATCATGAAAC AGCCAGTGTT
    TTGAGTTCTA GTAGCACACA TTCTGCTCCT CGAAGGCTGA CGAGTCATCT GGGAACCAAG
    GTGGAAATGG TGTATTCATT GTTGTCAATG CTTGGTACTC ATGATAAGGA TGATATGTCG
    CGAACTTTGC TAGCTATGTC TAGCTCCCAA GACAGCTGTA TATCCATGCG ACAGTCTGGA
    TGTCTTCCTC TCCTCATCCA GCTTTTACAT GGCAATGACA AAGACTCTGT GTTGTTGGGA
    AATTCCCGGG GCAGTAAAGA GGCTCGGGCC AGGGCCAGTG CAGCACTCCA CAACATCATT
    CACTCACAGC CTGATGACAA GAGAGGCAGG CGTGAAATCC GAGTCCTTCA TCTTTTGGAA
    CAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGTAGG
    AAGGCTACCC GGGGCATTTC ATCCCAGGAG CTAGGGCAGG GGCTTTCAGG GGGACTACAG
    GCCATTGCAG AATTATTGCA AGTGGACTGT GAAATGTATG GGCTTACTAA TGACCACTAC
    AGTATTACTT TAAGACGATA TGCAGGAATG GCTTTGACAA ACTTGACTTT CGGAGATGTA
    GCCAACAAGG CTACTCTCTG CTCTATGAAA GGCTGCATGA GAGCGCTCGT GGCTCAGCTC
    CAGTCTGAAA GCGAGGACTT GCAGCAGGTT ATTGCGAGTG TTTTGAGGAA TCTGTCTTGG
    AGAGCAGATG TAAATAGTAA AAAGACTTTG CGTGAAGTTG GAAGTGTGAA AGCATTGATG
    GAATGTGCTT TGGAAGTGAA AAAGGAATCA ACCCTCAAAA GTGTATTGAG TGCCTTATGG
    AACTTGTCAG CACACTGCAC TGAGAATAAA GCTGACATAT GTGCTGTGGA TGGTGCGCTT
    GCATTTTTGG TTGGCACTCT CACTTATCGC AGCCAGACCA ATACTTTAGC CATTATTGAA
    AGTGGAGGTG GTATATTACG GAATGTATCC AGCTTGATAG CTACAAATGA GGACCACAGG
    CAAATCCTAA GAGAGAATAA TTGCTTACAG ACCTTATTAC AACACTTGAA ATCTCACAGT
    TTGACAATAG TCAGCAATGC ATGCGGAACC TTGTGGAATC TCTCAGCAAG AAACCCTAAA
    GACCAGGAAG CATTGTGGGA CATGGGAGCA GTCAGCATGC TCAAGAACCT CATTCATTCA
    AAGCACAAGA TGATTGCTAT GGGAAGTGCT GCAGCTTTAA GGAATCTCAT GGCAAATAGA
    CCTGCAAAGT ATAAAGATGC CAATATCATG TCTCCTGGTT CAAGTTTGCC TTCTCTACAT
    GTCAGGAAAC AAAAGGCCCT GGAAGCAGAA TTAGATGCTC AGCATTTATC AGAAACTTTT
    GACAATATTG ACAATTTAAG TCCCAAGGCA TCTCATCGTA GCAAGCAGAG ACACAAGCAA
    AATCTCTATG GTGACTATGT TTTTGACACC AATCGACATG ATGATAACAG GTCAGACAAT
    TTTAATACTG GAAACATGAC TGTCCTATCA CCGTACTTAA ACACTACAGT ATTGCCCAGC
    TCTTCTTCAT CAAGGGGAAG TTTAGATAGC TCTCGTTCTG AGAAAGATAG AAGTCTGGAG
    AGAGAACGAG GTATTAGCCT AGGCAACTAT CACCCAGCAA CAGAAAACCC AGGAACCTCT
    TCAAAGCGAG GTTTGCAGAT TTCTACCACT GCAGCCCAGA TTGCCAAAGT CATGGAAGAA
    GTATCAGCTA TTCATACCTC TCAGGAAGAC AGAAGTTCTG GGTCTACCAC AGAACTACAC
    TGTGGGACAG ATGAGAGGAA TGCACTAAGA AGAAGCTCTA CCACCCACAC ACATGCAAAC
    ACGTACAACT TTACCAAGTC AGAAAACTCA AACAGAACAT GTCCAATACC ATATGCCAAA
    GTAGAATATA AGAGATCTTC AAATGATAGT TTAAATAGTG TCAGCAGTAG TGATGGTTAT
    GGTAAAAGAG GTCAGATGAA ACCTTCAATT GAATCCTATT CTGAAGATGA TGAAAGTAAA
    TTTTGCAGCT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AAATACATAG TGCAAATCAT
    ATGGATGATA ATGATGGAGA ACTAGATACA CCAATAAATT ACAGTCTCAA ATATTCCGAT
    GAACAGTTGA ACTCTGGGAG GCAGAGCCCT TCACAGAATG AAAGATGGGC AAGACCCAAA
    CATATACTAG AAGATGAAAT AAAACCGAAT GAGCAGAGAC AATCAAGGAG TCAAAGCACA
    GCTTATCCCG TGTATCCTGA GAGCACTGAT GATAAACACC TCAAGTTCCA ACCACACTTT
    GGGCAGCAAG AATGTGTTTC CCCATACAGG TCAAGAGCAG CCAATGGATC TGAAACAAAT
    CGAGTAGGCT CTAATCATGG AATTAGTCAA AATGTGAACC AGTCTTTGTG TCAAGAAGAT
    GACTATGAAG ACGATAAACC AACCAACTAT AGCGAACGTT ACTCTGAGGA AGGGCAGCAT
    GAGGAAGAAG AGAGGCCAAC CAATTATAGC ATAAAATACA GTGAAGAAAA ACATCACGTG
    GATCAGCCTA TTGATTATAG TTTAAAATAC ACCACAGACA TTCCTTCTTC ACAGAAACCA
    GCATTTTCAT TCTCAAAGAA TTCATCTGGA CAGAGCACAA AAACTGAACA CATCTCTTCA
    AGCAGCGAGA ATACATCCAC AACTTCATCT AATGCCAAGA GGCAGAATCA GCTGCATCCA
    AGCTCAGCAC AGAGCAGAAG TGGTCAGACC CCAAAAGCCA CCTCTTCCTC TTGCAAAGTC
    CCCTCTATCA ACCAAGAAAC AATACAGACT TACTGTGTAG AAGATACCCC AATATGTTTT
    TCAAGATGCA GTTCATTATC ATCTCTGTCA TCTGCTGAAG ATGAAGTAGG GTGTGATCAG
    ACAACACAAG AAGCAGAGTC TGCTAACACT CTGCAAATAG CTGAAATCAA GGACAACAGC
    GGACCTAGGT CAAATGAAGA TTCTGTGAGT AAAGTTCCAG CAGGGTCACA GCACATTAGA
    ACCAAATCCA GCAGACTCCA GGCTTCTGGT CTGTCTTCAG AGTCAGCCAG GCACAAAGCT
    GTTGAATTTT CTTCAGGGGC CAAATCTCCT TCCAAGAGCG GTGCTCAGAC ACCCAAAAGT
    CCACCAGAGC ACTACGTTCA GGAGACCCCA CTCATGTTTA GCAGATGTAC TTCAGTCAGT
    TCACTCGACA GTTTTGAGAG TCGCTCGATT GCCAGCTCTG TTCAGAGTGA ACCCTGCAGT
    GGAATGGTAA GTGGCATCAT AAGCCCCAGT GACCTCCCAG ATAGCCCTGG ACAAACCATG
    CCACCAAGCA GAAGCAAAAC CCCTCCGCCA CCGCCTCCGC CTCCTCCTCA GACAGTTCAA
    ACGAAGCAGG AAGTACCTAA AAATAAAGCA CCTAGTGCTG AAAAGAGAGA AAGTGGACCC
    AAGCAAGCTG CTGTAAATGC TGCAGTACAA AGGGTCCAGG TTCTTCCAGA GGCTGATACT
    CTGTTACATT TTGCCACAGA GAGTACTCCT GATGGATTTT CTTGTTCATC TAGCCTGAGC
    GCTCTGAGCC TTGATGAGCC ATTTATTCAG AAAGATGTGG AACTAAGAAT AATGCCTCCA
    GTTCAGGAAA ATGACAACGG GAATGAAACA GAAAGTGAGC AGCCTGAAGA ATCAAATGAA
    AACCAGGAAA AAGAGGCAGA AAAACCCACT GATTCTGAAA AAGATCTTTT AGATGAGTCA
    GATGATGATG ATATTGAAAT ACTAGAAGAG TGTATAATTT CTGCCATGCC AACAAAATCT
    TCACGCAAAG CCAAAAAACC AGCCCAGACT ACTTCAAAAT TACCTCCACC TGTGGCAAGG
    AAACCAAGTC AGCTCCCTGT ATACAAACTT CTGCCATCGC AAAACAGGTT ACAGGCACAA
    AAGCATGTTA GTTTTACACC AGGAGATGAT ATGCCACGGG TGTATTGTGT AGAAGGGACA
    CCTATAAACT TTTCCACAGC TACATCTCTG AGTGATCTAA CGATAGAATC CCCTCCAAAT
    GAGTTAGCTG CTGGAGAAGG GGTTAGAGCA GGGGCACAGT CAAGTGAGTT TGAAAAACGA
    GATACCATTC CTACTGAAGG CAGAAGTACA GATGAGGCTC AACGAGGGAA AGCCTCATCT
    GTCACTGTGC CTGAACTGGA CGACAGCAAA ACAGAAGAAG GTGATATTCT TGCAGAATGC
    ATTAATTCTG CCATGCCCAA AGGAAAAAGT CACAAGCCTT TCCGTGTGAA AAAGATCATG
    GACCAGGTCC AGCAAGCATC TATGTCTTCA TCTGGAACTA ACAAAAATCA ATTAGATGGT
    AAGACGAAGA AACCTACTTC ACCAGTAAAA CCTATACCAC AAAATACTGA ATACAGGACA
    CGTGTAAGAA AAAATACAGA CTCAAAAAAT AATCTAAATG CAGAAAGAAA TTTCTCGGAA
    AACAAAGACT CAAAGAAACA GCACTTGAAA AATAATTCCA AGGACTTCAA TGATAAACTG
    CCAAATAATG AAGACAGAGT CAGAGGAAGT TTTACTTTTG ATTCACCCCA TCATTACACG
    CCCATTGAAG GCACTCCGTA CTGTTTTTCA CGAAATGACT CTCTGAGTTC TCTAGATTTT
    GATGATGATG ATGTTGACCT TTCCAGGGAA AAGGCTGAAT TAAGAAAGGG GAAGGAAAAT
    AAAGAATCAG AAGCTAAAGT GACCAACCAC ACAGAACTAA CCTCAAACCA ACAATCAGCT
    AGTAAGACAC CAGCTGTTAC AAAGCAGCCA ATAAATAGAG GTCAGTCTAA ACCCGTGCTG
    CAGAAGCAGT CCACTTTTCC CCAGTCTTCC AAAGATATAC CAGACAGAGG GGCAGCAACA
    GATGAGAAAT TACAGAATTT CGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC
    TCTCTAAGCT CTCTTAGTGA CATTGATCAA GAAAACAACA ACAACAAGGA AAATGAACCT
    GTCAAAGAGA CAGAGCCCCC TGCCTCACAG GGAGAACCAG GTAAACCCCA GGCCTCAGGT
    TATGCTCCTA AATCGTTTCA CGTGGAAGAC ACCCCTGTTT GTTTCTCAAG AAACAGTTCT
    CTCAGTTCTC TTAGTATTGA TTCTGAAGAT GACCTGCTGC AGGAATGTAT AAGTTCTGCA
    ATGCCAAAAA AGAAAAAGCC TTCAAGGCTC AAGCCCGATA ATGAAAAGCA TAGTCCCAGA
    AATATGGGTG GCATATTAGC AGAAGATTTG ACACTCGATT TGAAAGATAT ACAGAGACCA
    GATTCAGAAC ATGGTTTATC CCCAGATTCA GAAAATTTTG ATTGGAAAGC TATTCAGGAA
    GGTGCAAATT CCATAGTAAG TAGTTTACAT CAAGCTGCTG CCGCTGCATG TTTATCTCGA
    CAAGCTTCAT CTGATTCAGA TTCTATCCTT TCATTGAAAT CGGGTATCTC TCTGGGATCA
    CCATTTCACC TTACACCTGA TCAAGAAGAA AAACCCTTTA CGAGTAATAA AGGTCCACGA
    ATTCTAAAAC CTGGGGAGAA AAGTACATTG GAAACTAAAA AAATAGAATC TGAAAATAAA
    GGAATAAAAG GAGGCAAAAA GGTTTATAAA AGTTTGATTA CTGGAAAAGT TCGATCTAAT
    TCGGAAATTT CGAGCCAAAT GAAACAACCC CTTCAAACAA ACATGCCTTC AATCTCTCGA
    GGTAGGACAA TGATTCATAT TCCAGGAGTT CGGAATAGCT CTTCAAGTAC AAGCCCAGTT
    TCTAAAAAAG GCCCGCCCCT CAAGACTCCA GCCTCCAAAA GCCCTAGTGA AGGTCAGCCG
    GCTACCACCT CTCCCAGAGG AACCAAGCCA TCAGTGAAGT CAGAATTAAG CCCTGTTACA
    AGGCAGGCAT CCCAGACAGC AGGATCAAAC AAAGGACCTT CTAGATCAGG ATCTAGAGAT
    TCCACTCCTT CAAGACCTGC CCAGCAACCA TTAAGTAGAC CAATGCAGTC TCCAGGGCGA
    AACTCAATCT CTCCTGGTAG AAATGGAATA AGTCCCCCTA ACAAATTATC TCAACTACCA
    AGGACGTCAT CCCCTAGTAC TGCTTCAACT AAGTCCTCAG GTTCTGGGAA AATGTCTTAC
    ACATCTCCTG GCAGACAGAT GAGCCAGCAG AACCTCACCA AACAAACAGG CTTATCCAAG
    AATGGCAGTG GTATCCCAAG AAGTGAATCT GCCTCCAAAG GGCTAAATCA AATGAGTAAT
    AGTAATGGAT CCAATAAAAA AGTAGAACTT TCTAGAATGT CTTCAACAAA GTCAAGTGGA
    AGTGAATCCG ATAGGTCAGA GAGACCTGTA TTAGTACGCC AATCAACTTT CATCAAAGAA
    GCTCCAAGCC CAACCCTAAG GAGAAAACTG GAGGAATCTG CTTCATTTGA ATCTCTTTCT
    CCATCTTCCA GACCCGATTC TCCCACACGG TCCCAGGCTC ATACTCCAGT TTTAAGTCCT
    TCCCTTCCTG ATATGTCTCT ATCTACACAT TCGTCTCTTC AGTCTGGTGG ATGGCGAAAA
    CTCCCACCTA ACCTCAGTCC CACCATAGAG TATAATGATG GAAGACCAGT AAAGCGCCAT
    GATATAGCAC GCTCTCATTC CGAAAGTCCT TCCAGACTTC CCATCAATAG GTCAGGGACC
    TGGAAACGTG AGCACAGCAA ACACTCATCA TCACTTCCTC GAGTAAGCAC TTGGAGAAGA
    ACTGGAAGTT CATCCTCAAT TCTTTCTGCT TCATCAGAAT CTAGTGAAAA GGCAAAAAGT
    GAGGACGAAA AACAAGTGAA CTCTATTTCA GGAAGCAAAC AAACTAAAGA AAACCAGGTA
    TCCACAAAAG GAACATGGAG AAAAATAAAA GAAAGTGAAA TTTCTCCCAC AAATAGTACT
    TCTCAGACCA CTTCTTCAGG TGCTGCAAAT GGTGCTGAAT CAAAGACTCT GATTTATCAA
    ATGGCACCTG CTGTTTCTAA AACAGAGGAT GTTTGGGTGA GAATTGAGGA TTGCCCCATT
    AACAACCCTA GATCTGGAAG ATCTCCAACA GGAAATACTC CCCCCGTGAT TGACACTGTT
    TCAGAAAAGG GAAACCCGAA CCCTAAAGAT TCAAAAGATA ATCAGGGAAA ACAAAATGTG
    AGCAATGGTA GTGCCCCTAC ACGCACCATG GGTCTGGAAA ACCGCCTGAA TTCCTTTATT
    CAGGTAGATC CTCCAGACCA AAAAGGAACT GAGACAAAAC CGGGACACAG TAATAACCCT
    GTCCCTGCAT CCGAGACTAG TGAAAGTTCT ATAGCCGAGC GTACCCCGTT TAGTTCTAGC
    AGCTCAAGCA AGCACAGTTC ACCAAGTGGG ACTGTCGCCG CCAGGGTGAG TCCTTTTAAT
    TACAACCCAA GCCCAAGGAA GAGCAGCACA GATGGCACTT CAGCCCGACC GTCTCAGATC
    CCAACGCCAG TGAGCAACAA CACAAAGAAA CGCGACTCAA AACCCGACAG CACAGAACCC
    AGCGGGACTC AAAGTCCTAA ACGCCATTCT GGGTCTTACC TTGTGACATC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22863
    Clone ID Related Accession (Same CDS sequence) XM_005211196.3
    Accession Version XM_005211196.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGG
    GGACTACAGG CCATTGCAGA ATTATTGCAA GTGGACTGTG AAATGTATGG GCTTACTAAT
    GACCACTACA GTATTACTTT AAGACGATAT GCAGGAATGG CTTTGACAAA CTTGACTTTC
    GGAGATGTAG CCAACAAGGC TACTCTCTGC TCTATGAAAG GCTGCATGAG AGCGCTCGTG
    GCTCAGCTCC AGTCTGAAAG CGAGGACTTG CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT
    CTGTCTTGGA GAGCAGATGT AAATAGTAAA AAGACTTTGC GTGAAGTTGG AAGTGTGAAA
    GCATTGATGG AATGTGCTTT GGAAGTGAAA AAGGAATCAA CCCTCAAAAG TGTATTGAGT
    GCCTTATGGA ACTTGTCAGC ACACTGCACT GAGAATAAAG CTGACATATG TGCTGTGGAT
    GGTGCGCTTG CATTTTTGGT TGGCACTCTC ACTTATCGCA GCCAGACCAA TACTTTAGCC
    ATTATTGAAA GTGGAGGTGG TATATTACGG AATGTATCCA GCTTGATAGC TACAAATGAG
    GACCACAGGC AAATCCTAAG AGAGAATAAT TGCTTACAGA CCTTATTACA ACACTTGAAA
    TCTCACAGTT TGACAATAGT CAGCAATGCA TGCGGAACCT TGTGGAATCT CTCAGCAAGA
    AACCCTAAAG ACCAGGAAGC ATTGTGGGAC ATGGGAGCAG TCAGCATGCT CAAGAACCTC
    ATTCATTCAA AGCACAAGAT GATTGCTATG GGAAGTGCTG CAGCTTTAAG GAATCTCATG
    GCAAATAGAC CTGCAAAGTA TAAAGATGCC AATATCATGT CTCCTGGTTC AAGTTTGCCT
    TCTCTACATG TCAGGAAACA AAAGGCCCTG GAAGCAGAAT TAGATGCTCA GCATTTATCA
    GAAACTTTTG ACAATATTGA CAATTTAAGT CCCAAGGCAT CTCATCGTAG CAAGCAGAGA
    CACAAGCAAA ATCTCTATGG TGACTATGTT TTTGACACCA ATCGACATGA TGATAACAGG
    TCAGACAATT TTAATACTGG AAACATGACT GTCCTATCAC CGTACTTAAA CACTACAGTA
    TTGCCCAGCT CTTCTTCATC AAGGGGAAGT TTAGATAGCT CTCGTTCTGA GAAAGATAGA
    AGTCTGGAGA GAGAACGAGG TATTAGCCTA GGCAACTATC ACCCAGCAAC AGAAAACCCA
    GGAACCTCTT CAAAGCGAGG TTTGCAGATT TCTACCACTG CAGCCCAGAT TGCCAAAGTC
    ATGGAAGAAG TATCAGCTAT TCATACCTCT CAGGAAGACA GAAGTTCTGG GTCTACCACA
    GAACTACACT GTGGGACAGA TGAGAGGAAT GCACTAAGAA GAAGCTCTAC CACCCACACA
    CATGCAAACA CGTACAACTT TACCAAGTCA GAAAACTCAA ACAGAACATG TCCAATACCA
    TATGCCAAAG TAGAATATAA GAGATCTTCA AATGATAGTT TAAATAGTGT CAGCAGTAGT
    GATGGTTATG GTAAAAGAGG TCAGATGAAA CCTTCAATTG AATCCTATTC TGAAGATGAT
    GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT CCAGCTGACC TAGCCCATAA AATACATAGT
    GCAAATCATA TGGATGATAA TGATGGAGAA CTAGATACAC CAATAAATTA CAGTCTCAAA
    TATTCCGATG AACAGTTGAA CTCTGGGAGG CAGAGCCCTT CACAGAATGA AAGATGGGCA
    AGACCCAAAC ATATACTAGA AGATGAAATA AAACCGAATG AGCAGAGACA ATCAAGGAGT
    CAAAGCACAG CTTATCCCGT GTATCCTGAG AGCACTGATG ATAAACACCT CAAGTTCCAA
    CCACACTTTG GGCAGCAAGA ATGTGTTTCC CCATACAGGT CAAGAGCAGC CAATGGATCT
    GAAACAAATC GAGTAGGCTC TAATCATGGA ATTAGTCAAA ATGTGAACCA GTCTTTGTGT
    CAAGAAGATG ACTATGAAGA CGATAAACCA ACCAACTATA GCGAACGTTA CTCTGAGGAA
    GGGCAGCATG AGGAAGAAGA GAGGCCAACC AATTATAGCA TAAAATACAG TGAAGAAAAA
    CATCACGTGG ATCAGCCTAT TGATTATAGT TTAAAATACA CCACAGACAT TCCTTCTTCA
    CAGAAACCAG CATTTTCATT CTCAAAGAAT TCATCTGGAC AGAGCACAAA AACTGAACAC
    ATCTCTTCAA GCAGCGAGAA TACATCCACA ACTTCATCTA ATGCCAAGAG GCAGAATCAG
    CTGCATCCAA GCTCAGCACA GAGCAGAAGT GGTCAGACCC CAAAAGCCAC CTCTTCCTCT
    TGCAAAGTCC CCTCTATCAA CCAAGAAACA ATACAGACTT ACTGTGTAGA AGATACCCCA
    ATATGTTTTT CAAGATGCAG TTCATTATCA TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG
    TGTGATCAGA CAACACAAGA AGCAGAGTCT GCTAACACTC TGCAAATAGC TGAAATCAAG
    GACAACAGCG GACCTAGGTC AAATGAAGAT TCTGTGAGTA AAGTTCCAGC AGGGTCACAG
    CACATTAGAA CCAAATCCAG CAGACTCCAG GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG
    CACAAAGCTG TTGAATTTTC TTCAGGGGCC AAATCTCCTT CCAAGAGCGG TGCTCAGACA
    CCCAAAAGTC CACCAGAGCA CTACGTTCAG GAGACCCCAC TCATGTTTAG CAGATGTACT
    TCAGTCAGTT CACTCGACAG TTTTGAGAGT CGCTCGATTG CCAGCTCTGT TCAGAGTGAA
    CCCTGCAGTG GAATGGTAAG TGGCATCATA AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA
    CAAACCATGC CACCAAGCAG AAGCAAAACC CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG
    ACAGTTCAAA CGAAGCAGGA AGTACCTAAA AATAAAGCAC CTAGTGCTGA AAAGAGAGAA
    AGTGGACCCA AGCAAGCTGC TGTAAATGCT GCAGTACAAA GGGTCCAGGT TCTTCCAGAG
    GCTGATACTC TGTTACATTT TGCCACAGAG AGTACTCCTG ATGGATTTTC TTGTTCATCT
    AGCCTGAGCG CTCTGAGCCT TGATGAGCCA TTTATTCAGA AAGATGTGGA ACTAAGAATA
    ATGCCTCCAG TTCAGGAAAA TGACAACGGG AATGAAACAG AAAGTGAGCA GCCTGAAGAA
    TCAAATGAAA ACCAGGAAAA AGAGGCAGAA AAACCCACTG ATTCTGAAAA AGATCTTTTA
    GATGAGTCAG ATGATGATGA TATTGAAATA CTAGAAGAGT GTATAATTTC TGCCATGCCA
    ACAAAATCTT CACGCAAAGC CAAAAAACCA GCCCAGACTA CTTCAAAATT ACCTCCACCT
    GTGGCAAGGA AACCAAGTCA GCTCCCTGTA TACAAACTTC TGCCATCGCA AAACAGGTTA
    CAGGCACAAA AGCATGTTAG TTTTACACCA GGAGATGATA TGCCACGGGT GTATTGTGTA
    GAAGGGACAC CTATAAACTT TTCCACAGCT ACATCTCTGA GTGATCTAAC GATAGAATCC
    CCTCCAAATG AGTTAGCTGC TGGAGAAGGG GTTAGAGCAG GGGCACAGTC AAGTGAGTTT
    GAAAAACGAG ATACCATTCC TACTGAAGGC AGAAGTACAG ATGAGGCTCA ACGAGGGAAA
    GCCTCATCTG TCACTGTGCC TGAACTGGAC GACAGCAAAA CAGAAGAAGG TGATATTCTT
    GCAGAATGCA TTAATTCTGC CATGCCCAAA GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA
    AAGATCATGG ACCAGGTCCA GCAAGCATCT ATGTCTTCAT CTGGAACTAA CAAAAATCAA
    TTAGATGGTA AGACGAAGAA ACCTACTTCA CCAGTAAAAC CTATACCACA AAATACTGAA
    TACAGGACAC GTGTAAGAAA AAATACAGAC TCAAAAAATA ATCTAAATGC AGAAAGAAAT
    TTCTCGGAAA ACAAAGACTC AAAGAAACAG CACTTGAAAA ATAATTCCAA GGACTTCAAT
    GATAAACTGC CAAATAATGA AGACAGAGTC AGAGGAAGTT TTACTTTTGA TTCACCCCAT
    CATTACACGC CCATTGAAGG CACTCCGTAC TGTTTTTCAC GAAATGACTC TCTGAGTTCT
    CTAGATTTTG ATGATGATGA TGTTGACCTT TCCAGGGAAA AGGCTGAATT AAGAAAGGGG
    AAGGAAAATA AAGAATCAGA AGCTAAAGTG ACCAACCACA CAGAACTAAC CTCAAACCAA
    CAATCAGCTA GTAAGACACC AGCTGTTACA AAGCAGCCAA TAAATAGAGG TCAGTCTAAA
    CCCGTGCTGC AGAAGCAGTC CACTTTTCCC CAGTCTTCCA AAGATATACC AGACAGAGGG
    GCAGCAACAG ATGAGAAATT ACAGAATTTC GCTATTGAAA ATACTCCAGT TTGCTTTTCT
    CGAAATTCCT CTCTAAGCTC TCTTAGTGAC ATTGATCAAG AAAACAACAA CAACAAGGAA
    AATGAACCTG TCAAAGAGAC AGAGCCCCCT GCCTCACAGG GAGAACCAGG TAAACCCCAG
    GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC GTGGAAGACA CCCCTGTTTG TTTCTCAAGA
    AACAGTTCTC TCAGTTCTCT TAGTATTGAT TCTGAAGATG ACCTGCTGCA GGAATGTATA
    AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT TCAAGGCTCA AGCCCGATAA TGAAAAGCAT
    AGTCCCAGAA ATATGGGTGG CATATTAGCA GAAGATTTGA CACTCGATTT GAAAGATATA
    CAGAGACCAG ATTCAGAACA TGGTTTATCC CCAGATTCAG AAAATTTTGA TTGGAAAGCT
    ATTCAGGAAG GTGCAAATTC CATAGTAAGT AGTTTACATC AAGCTGCTGC CGCTGCATGT
    TTATCTCGAC AAGCTTCATC TGATTCAGAT TCTATCCTTT CATTGAAATC GGGTATCTCT
    CTGGGATCAC CATTTCACCT TACACCTGAT CAAGAAGAAA AACCCTTTAC GAGTAATAAA
    GGTCCACGAA TTCTAAAACC TGGGGAGAAA AGTACATTGG AAACTAAAAA AATAGAATCT
    GAAAATAAAG GAATAAAAGG AGGCAAAAAG GTTTATAAAA GTTTGATTAC TGGAAAAGTT
    CGATCTAATT CGGAAATTTC GAGCCAAATG AAACAACCCC TTCAAACAAA CATGCCTTCA
    ATCTCTCGAG GTAGGACAAT GATTCATATT CCAGGAGTTC GGAATAGCTC TTCAAGTACA
    AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC AAGACTCCAG CCTCCAAAAG CCCTAGTGAA
    GGTCAGCCGG CTACCACCTC TCCCAGAGGA ACCAAGCCAT CAGTGAAGTC AGAATTAAGC
    CCTGTTACAA GGCAGGCATC CCAGACAGCA GGATCAAACA AAGGACCTTC TAGATCAGGA
    TCTAGAGATT CCACTCCTTC AAGACCTGCC CAGCAACCAT TAAGTAGACC AATGCAGTCT
    CCAGGGCGAA ACTCAATCTC TCCTGGTAGA AATGGAATAA GTCCCCCTAA CAAATTATCT
    CAACTACCAA GGACGTCATC CCCTAGTACT GCTTCAACTA AGTCCTCAGG TTCTGGGAAA
    ATGTCTTACA CATCTCCTGG CAGACAGATG AGCCAGCAGA ACCTCACCAA ACAAACAGGC
    TTATCCAAGA ATGGCAGTGG TATCCCAAGA AGTGAATCTG CCTCCAAAGG GCTAAATCAA
    ATGAGTAATA GTAATGGATC CAATAAAAAA GTAGAACTTT CTAGAATGTC TTCAACAAAG
    TCAAGTGGAA GTGAATCCGA TAGGTCAGAG AGACCTGTAT TAGTACGCCA ATCAACTTTC
    ATCAAAGAAG CTCCAAGCCC AACCCTAAGG AGAAAACTGG AGGAATCTGC TTCATTTGAA
    TCTCTTTCTC CATCTTCCAG ACCCGATTCT CCCACACGGT CCCAGGCTCA TACTCCAGTT
    TTAAGTCCTT CCCTTCCTGA TATGTCTCTA TCTACACATT CGTCTCTTCA GTCTGGTGGA
    TGGCGAAAAC TCCCACCTAA CCTCAGTCCC ACCATAGAGT ATAATGATGG AAGACCAGTA
    AAGCGCCATG ATATAGCACG CTCTCATTCC GAAAGTCCTT CCAGACTTCC CATCAATAGG
    TCAGGGACCT GGAAACGTGA GCACAGCAAA CACTCATCAT CACTTCCTCG AGTAAGCACT
    TGGAGAAGAA CTGGAAGTTC ATCCTCAATT CTTTCTGCTT CATCAGAATC TAGTGAAAAG
    GCAAAAAGTG AGGACGAAAA ACAAGTGAAC TCTATTTCAG GAAGCAAACA AACTAAAGAA
    AACCAGGTAT CCACAAAAGG AACATGGAGA AAAATAAAAG AAAGTGAAAT TTCTCCCACA
    AATAGTACTT CTCAGACCAC TTCTTCAGGT GCTGCAAATG GTGCTGAATC AAAGACTCTG
    ATTTATCAAA TGGCACCTGC TGTTTCTAAA ACAGAGGATG TTTGGGTGAG AATTGAGGAT
    TGCCCCATTA ACAACCCTAG ATCTGGAAGA TCTCCAACAG GAAATACTCC CCCCGTGATT
    GACACTGTTT CAGAAAAGGG AAACCCGAAC CCTAAAGATT CAAAAGATAA TCAGGGAAAA
    CAAAATGTGA GCAATGGTAG TGCCCCTACA CGCACCATGG GTCTGGAAAA CCGCCTGAAT
    TCCTTTATTC AGGTAGATCC TCCAGACCAA AAAGGAACTG AGACAAAACC GGGACACAGT
    AATAACCCTG TCCCTGCATC CGAGACTAGT GAAAGTTCTA TAGCCGAGCG TACCCCGTTT
    AGTTCTAGCA GCTCAAGCAA GCACAGTTCA CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT
    CCTTTTAATT ACAACCCAAG CCCAAGGAAG AGCAGCACAG ATGGCACTTC AGCCCGACCG
    TCTCAGATCC CAACGCCAGT GAGCAACAAC ACAAAGAAAC GCGACTCAAA ACCCGACAGC
    ACAGAACCCA GCGGGACTCA AAGTCCTAAA CGCCATTCTG GGTCTTACCT TGTGACATCT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211253.1
    CDS142..8727
    Translation

    Target ORF information:

    RefSeq Version XM_005211196.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211196.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGG
    GGACTACAGG CCATTGCAGA ATTATTGCAA GTGGACTGTG AAATGTATGG GCTTACTAAT
    GACCACTACA GTATTACTTT AAGACGATAT GCAGGAATGG CTTTGACAAA CTTGACTTTC
    GGAGATGTAG CCAACAAGGC TACTCTCTGC TCTATGAAAG GCTGCATGAG AGCGCTCGTG
    GCTCAGCTCC AGTCTGAAAG CGAGGACTTG CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT
    CTGTCTTGGA GAGCAGATGT AAATAGTAAA AAGACTTTGC GTGAAGTTGG AAGTGTGAAA
    GCATTGATGG AATGTGCTTT GGAAGTGAAA AAGGAATCAA CCCTCAAAAG TGTATTGAGT
    GCCTTATGGA ACTTGTCAGC ACACTGCACT GAGAATAAAG CTGACATATG TGCTGTGGAT
    GGTGCGCTTG CATTTTTGGT TGGCACTCTC ACTTATCGCA GCCAGACCAA TACTTTAGCC
    ATTATTGAAA GTGGAGGTGG TATATTACGG AATGTATCCA GCTTGATAGC TACAAATGAG
    GACCACAGGC AAATCCTAAG AGAGAATAAT TGCTTACAGA CCTTATTACA ACACTTGAAA
    TCTCACAGTT TGACAATAGT CAGCAATGCA TGCGGAACCT TGTGGAATCT CTCAGCAAGA
    AACCCTAAAG ACCAGGAAGC ATTGTGGGAC ATGGGAGCAG TCAGCATGCT CAAGAACCTC
    ATTCATTCAA AGCACAAGAT GATTGCTATG GGAAGTGCTG CAGCTTTAAG GAATCTCATG
    GCAAATAGAC CTGCAAAGTA TAAAGATGCC AATATCATGT CTCCTGGTTC AAGTTTGCCT
    TCTCTACATG TCAGGAAACA AAAGGCCCTG GAAGCAGAAT TAGATGCTCA GCATTTATCA
    GAAACTTTTG ACAATATTGA CAATTTAAGT CCCAAGGCAT CTCATCGTAG CAAGCAGAGA
    CACAAGCAAA ATCTCTATGG TGACTATGTT TTTGACACCA ATCGACATGA TGATAACAGG
    TCAGACAATT TTAATACTGG AAACATGACT GTCCTATCAC CGTACTTAAA CACTACAGTA
    TTGCCCAGCT CTTCTTCATC AAGGGGAAGT TTAGATAGCT CTCGTTCTGA GAAAGATAGA
    AGTCTGGAGA GAGAACGAGG TATTAGCCTA GGCAACTATC ACCCAGCAAC AGAAAACCCA
    GGAACCTCTT CAAAGCGAGG TTTGCAGATT TCTACCACTG CAGCCCAGAT TGCCAAAGTC
    ATGGAAGAAG TATCAGCTAT TCATACCTCT CAGGAAGACA GAAGTTCTGG GTCTACCACA
    GAACTACACT GTGGGACAGA TGAGAGGAAT GCACTAAGAA GAAGCTCTAC CACCCACACA
    CATGCAAACA CGTACAACTT TACCAAGTCA GAAAACTCAA ACAGAACATG TCCAATACCA
    TATGCCAAAG TAGAATATAA GAGATCTTCA AATGATAGTT TAAATAGTGT CAGCAGTAGT
    GATGGTTATG GTAAAAGAGG TCAGATGAAA CCTTCAATTG AATCCTATTC TGAAGATGAT
    GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT CCAGCTGACC TAGCCCATAA AATACATAGT
    GCAAATCATA TGGATGATAA TGATGGAGAA CTAGATACAC CAATAAATTA CAGTCTCAAA
    TATTCCGATG AACAGTTGAA CTCTGGGAGG CAGAGCCCTT CACAGAATGA AAGATGGGCA
    AGACCCAAAC ATATACTAGA AGATGAAATA AAACCGAATG AGCAGAGACA ATCAAGGAGT
    CAAAGCACAG CTTATCCCGT GTATCCTGAG AGCACTGATG ATAAACACCT CAAGTTCCAA
    CCACACTTTG GGCAGCAAGA ATGTGTTTCC CCATACAGGT CAAGAGCAGC CAATGGATCT
    GAAACAAATC GAGTAGGCTC TAATCATGGA ATTAGTCAAA ATGTGAACCA GTCTTTGTGT
    CAAGAAGATG ACTATGAAGA CGATAAACCA ACCAACTATA GCGAACGTTA CTCTGAGGAA
    GGGCAGCATG AGGAAGAAGA GAGGCCAACC AATTATAGCA TAAAATACAG TGAAGAAAAA
    CATCACGTGG ATCAGCCTAT TGATTATAGT TTAAAATACA CCACAGACAT TCCTTCTTCA
    CAGAAACCAG CATTTTCATT CTCAAAGAAT TCATCTGGAC AGAGCACAAA AACTGAACAC
    ATCTCTTCAA GCAGCGAGAA TACATCCACA ACTTCATCTA ATGCCAAGAG GCAGAATCAG
    CTGCATCCAA GCTCAGCACA GAGCAGAAGT GGTCAGACCC CAAAAGCCAC CTCTTCCTCT
    TGCAAAGTCC CCTCTATCAA CCAAGAAACA ATACAGACTT ACTGTGTAGA AGATACCCCA
    ATATGTTTTT CAAGATGCAG TTCATTATCA TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG
    TGTGATCAGA CAACACAAGA AGCAGAGTCT GCTAACACTC TGCAAATAGC TGAAATCAAG
    GACAACAGCG GACCTAGGTC AAATGAAGAT TCTGTGAGTA AAGTTCCAGC AGGGTCACAG
    CACATTAGAA CCAAATCCAG CAGACTCCAG GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG
    CACAAAGCTG TTGAATTTTC TTCAGGGGCC AAATCTCCTT CCAAGAGCGG TGCTCAGACA
    CCCAAAAGTC CACCAGAGCA CTACGTTCAG GAGACCCCAC TCATGTTTAG CAGATGTACT
    TCAGTCAGTT CACTCGACAG TTTTGAGAGT CGCTCGATTG CCAGCTCTGT TCAGAGTGAA
    CCCTGCAGTG GAATGGTAAG TGGCATCATA AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA
    CAAACCATGC CACCAAGCAG AAGCAAAACC CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG
    ACAGTTCAAA CGAAGCAGGA AGTACCTAAA AATAAAGCAC CTAGTGCTGA AAAGAGAGAA
    AGTGGACCCA AGCAAGCTGC TGTAAATGCT GCAGTACAAA GGGTCCAGGT TCTTCCAGAG
    GCTGATACTC TGTTACATTT TGCCACAGAG AGTACTCCTG ATGGATTTTC TTGTTCATCT
    AGCCTGAGCG CTCTGAGCCT TGATGAGCCA TTTATTCAGA AAGATGTGGA ACTAAGAATA
    ATGCCTCCAG TTCAGGAAAA TGACAACGGG AATGAAACAG AAAGTGAGCA GCCTGAAGAA
    TCAAATGAAA ACCAGGAAAA AGAGGCAGAA AAACCCACTG ATTCTGAAAA AGATCTTTTA
    GATGAGTCAG ATGATGATGA TATTGAAATA CTAGAAGAGT GTATAATTTC TGCCATGCCA
    ACAAAATCTT CACGCAAAGC CAAAAAACCA GCCCAGACTA CTTCAAAATT ACCTCCACCT
    GTGGCAAGGA AACCAAGTCA GCTCCCTGTA TACAAACTTC TGCCATCGCA AAACAGGTTA
    CAGGCACAAA AGCATGTTAG TTTTACACCA GGAGATGATA TGCCACGGGT GTATTGTGTA
    GAAGGGACAC CTATAAACTT TTCCACAGCT ACATCTCTGA GTGATCTAAC GATAGAATCC
    CCTCCAAATG AGTTAGCTGC TGGAGAAGGG GTTAGAGCAG GGGCACAGTC AAGTGAGTTT
    GAAAAACGAG ATACCATTCC TACTGAAGGC AGAAGTACAG ATGAGGCTCA ACGAGGGAAA
    GCCTCATCTG TCACTGTGCC TGAACTGGAC GACAGCAAAA CAGAAGAAGG TGATATTCTT
    GCAGAATGCA TTAATTCTGC CATGCCCAAA GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA
    AAGATCATGG ACCAGGTCCA GCAAGCATCT ATGTCTTCAT CTGGAACTAA CAAAAATCAA
    TTAGATGGTA AGACGAAGAA ACCTACTTCA CCAGTAAAAC CTATACCACA AAATACTGAA
    TACAGGACAC GTGTAAGAAA AAATACAGAC TCAAAAAATA ATCTAAATGC AGAAAGAAAT
    TTCTCGGAAA ACAAAGACTC AAAGAAACAG CACTTGAAAA ATAATTCCAA GGACTTCAAT
    GATAAACTGC CAAATAATGA AGACAGAGTC AGAGGAAGTT TTACTTTTGA TTCACCCCAT
    CATTACACGC CCATTGAAGG CACTCCGTAC TGTTTTTCAC GAAATGACTC TCTGAGTTCT
    CTAGATTTTG ATGATGATGA TGTTGACCTT TCCAGGGAAA AGGCTGAATT AAGAAAGGGG
    AAGGAAAATA AAGAATCAGA AGCTAAAGTG ACCAACCACA CAGAACTAAC CTCAAACCAA
    CAATCAGCTA GTAAGACACC AGCTGTTACA AAGCAGCCAA TAAATAGAGG TCAGTCTAAA
    CCCGTGCTGC AGAAGCAGTC CACTTTTCCC CAGTCTTCCA AAGATATACC AGACAGAGGG
    GCAGCAACAG ATGAGAAATT ACAGAATTTC GCTATTGAAA ATACTCCAGT TTGCTTTTCT
    CGAAATTCCT CTCTAAGCTC TCTTAGTGAC ATTGATCAAG AAAACAACAA CAACAAGGAA
    AATGAACCTG TCAAAGAGAC AGAGCCCCCT GCCTCACAGG GAGAACCAGG TAAACCCCAG
    GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC GTGGAAGACA CCCCTGTTTG TTTCTCAAGA
    AACAGTTCTC TCAGTTCTCT TAGTATTGAT TCTGAAGATG ACCTGCTGCA GGAATGTATA
    AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT TCAAGGCTCA AGCCCGATAA TGAAAAGCAT
    AGTCCCAGAA ATATGGGTGG CATATTAGCA GAAGATTTGA CACTCGATTT GAAAGATATA
    CAGAGACCAG ATTCAGAACA TGGTTTATCC CCAGATTCAG AAAATTTTGA TTGGAAAGCT
    ATTCAGGAAG GTGCAAATTC CATAGTAAGT AGTTTACATC AAGCTGCTGC CGCTGCATGT
    TTATCTCGAC AAGCTTCATC TGATTCAGAT TCTATCCTTT CATTGAAATC GGGTATCTCT
    CTGGGATCAC CATTTCACCT TACACCTGAT CAAGAAGAAA AACCCTTTAC GAGTAATAAA
    GGTCCACGAA TTCTAAAACC TGGGGAGAAA AGTACATTGG AAACTAAAAA AATAGAATCT
    GAAAATAAAG GAATAAAAGG AGGCAAAAAG GTTTATAAAA GTTTGATTAC TGGAAAAGTT
    CGATCTAATT CGGAAATTTC GAGCCAAATG AAACAACCCC TTCAAACAAA CATGCCTTCA
    ATCTCTCGAG GTAGGACAAT GATTCATATT CCAGGAGTTC GGAATAGCTC TTCAAGTACA
    AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC AAGACTCCAG CCTCCAAAAG CCCTAGTGAA
    GGTCAGCCGG CTACCACCTC TCCCAGAGGA ACCAAGCCAT CAGTGAAGTC AGAATTAAGC
    CCTGTTACAA GGCAGGCATC CCAGACAGCA GGATCAAACA AAGGACCTTC TAGATCAGGA
    TCTAGAGATT CCACTCCTTC AAGACCTGCC CAGCAACCAT TAAGTAGACC AATGCAGTCT
    CCAGGGCGAA ACTCAATCTC TCCTGGTAGA AATGGAATAA GTCCCCCTAA CAAATTATCT
    CAACTACCAA GGACGTCATC CCCTAGTACT GCTTCAACTA AGTCCTCAGG TTCTGGGAAA
    ATGTCTTACA CATCTCCTGG CAGACAGATG AGCCAGCAGA ACCTCACCAA ACAAACAGGC
    TTATCCAAGA ATGGCAGTGG TATCCCAAGA AGTGAATCTG CCTCCAAAGG GCTAAATCAA
    ATGAGTAATA GTAATGGATC CAATAAAAAA GTAGAACTTT CTAGAATGTC TTCAACAAAG
    TCAAGTGGAA GTGAATCCGA TAGGTCAGAG AGACCTGTAT TAGTACGCCA ATCAACTTTC
    ATCAAAGAAG CTCCAAGCCC AACCCTAAGG AGAAAACTGG AGGAATCTGC TTCATTTGAA
    TCTCTTTCTC CATCTTCCAG ACCCGATTCT CCCACACGGT CCCAGGCTCA TACTCCAGTT
    TTAAGTCCTT CCCTTCCTGA TATGTCTCTA TCTACACATT CGTCTCTTCA GTCTGGTGGA
    TGGCGAAAAC TCCCACCTAA CCTCAGTCCC ACCATAGAGT ATAATGATGG AAGACCAGTA
    AAGCGCCATG ATATAGCACG CTCTCATTCC GAAAGTCCTT CCAGACTTCC CATCAATAGG
    TCAGGGACCT GGAAACGTGA GCACAGCAAA CACTCATCAT CACTTCCTCG AGTAAGCACT
    TGGAGAAGAA CTGGAAGTTC ATCCTCAATT CTTTCTGCTT CATCAGAATC TAGTGAAAAG
    GCAAAAAGTG AGGACGAAAA ACAAGTGAAC TCTATTTCAG GAAGCAAACA AACTAAAGAA
    AACCAGGTAT CCACAAAAGG AACATGGAGA AAAATAAAAG AAAGTGAAAT TTCTCCCACA
    AATAGTACTT CTCAGACCAC TTCTTCAGGT GCTGCAAATG GTGCTGAATC AAAGACTCTG
    ATTTATCAAA TGGCACCTGC TGTTTCTAAA ACAGAGGATG TTTGGGTGAG AATTGAGGAT
    TGCCCCATTA ACAACCCTAG ATCTGGAAGA TCTCCAACAG GAAATACTCC CCCCGTGATT
    GACACTGTTT CAGAAAAGGG AAACCCGAAC CCTAAAGATT CAAAAGATAA TCAGGGAAAA
    CAAAATGTGA GCAATGGTAG TGCCCCTACA CGCACCATGG GTCTGGAAAA CCGCCTGAAT
    TCCTTTATTC AGGTAGATCC TCCAGACCAA AAAGGAACTG AGACAAAACC GGGACACAGT
    AATAACCCTG TCCCTGCATC CGAGACTAGT GAAAGTTCTA TAGCCGAGCG TACCCCGTTT
    AGTTCTAGCA GCTCAAGCAA GCACAGTTCA CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT
    CCTTTTAATT ACAACCCAAG CCCAAGGAAG AGCAGCACAG ATGGCACTTC AGCCCGACCG
    TCTCAGATCC CAACGCCAGT GAGCAACAAC ACAAAGAAAC GCGACTCAAA ACCCGACAGC
    ACAGAACCCA GCGGGACTCA AAGTCCTAAA CGCCATTCTG GGTCTTACCT TGTGACATCT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22864
    Clone ID Related Accession (Same CDS sequence) XM_005211197.3 , XM_015472985.1
    Accession Version XM_005211197.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211197.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
    1681
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    1801
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    1981
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    8401
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    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211254.1
    CDS121..8676
    Translation

    Target ORF information:

    RefSeq Version XM_005211197.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211197.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22864
    Clone ID Related Accession (Same CDS sequence) XM_005211197.3 , XM_015472985.1
    Accession Version XM_015472985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328471.1
    CDS21..8576
    Translation

    Target ORF information:

    RefSeq Version XM_015472985.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGGTGGAAA TGGTGTATTC ATTGTTGTCA
    ATGCTTGGTA CTCATGATAA GGATGATATG TCGCGAACTT TGCTAGCTAT GTCTAGCTCC
    CAAGACAGCT GTATATCCAT GCGACAGTCT GGATGTCTTC CTCTCCTCAT CCAGCTTTTA
    CATGGCAATG ACAAAGACTC TGTGTTGTTG GGAAATTCCC GGGGCAGTAA AGAGGCTCGG
    GCCAGGGCCA GTGCAGCACT CCACAACATC ATTCACTCAC AGCCTGATGA CAAGAGAGGC
    AGGCGTGAAA TCCGAGTCCT TCATCTTTTG GAACAGATAC GAGCTTACTG TGAAACCTGT
    TGGGAGTGGC AGGAAGCCCA TGAACAAGGG ATGGACCAGG ACAAAAATCC AATGCCAGCT
    CCTGTTGAAC ATCAGATCTG CCCTGCTGTC TGTGTTCTAA TGAAGCTTTC GTTTGATGAA
    GAGCATAGAC ATGCCATGAA TGAGCTTGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22868
    Clone ID Related Accession (Same CDS sequence) XM_005211202.3
    Accession Version XM_005211202.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211202.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGGGGA
    CTACAGGCCA TTGCAGAATT ATTGCAAGTG GACTGTGAAA TGTATGGGCT TACTAATGAC
    CACTACAGTA TTACTTTAAG ACGATATGCA GGAATGGCTT TGACAAACTT GACTTTCGGA
    GATGTAGCCA ACAAGGCTAC TCTCTGCTCT ATGAAAGGCT GCATGAGAGC GCTCGTGGCT
    CAGCTCCAGT CTGAAAGCGA GGACTTGCAG CAGGTTATTG CGAGTGTTTT GAGGAATCTG
    TCTTGGAGAG CAGATGTAAA TAGTAAAAAG ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA
    TTGATGGAAT GTGCTTTGGA AGTGAAAAAG GAATCAACCC TCAAAAGTGT ATTGAGTGCC
    TTATGGAACT TGTCAGCACA CTGCACTGAG AATAAAGCTG ACATATGTGC TGTGGATGGT
    GCGCTTGCAT TTTTGGTTGG CACTCTCACT TATCGCAGCC AGACCAATAC TTTAGCCATT
    ATTGAAAGTG GAGGTGGTAT ATTACGGAAT GTATCCAGCT TGATAGCTAC AAATGAGGAC
    CACAGGCAAA TCCTAAGAGA GAATAATTGC TTACAGACCT TATTACAACA CTTGAAATCT
    CACAGTTTGA CAATAGTCAG CAATGCATGC GGAACCTTGT GGAATCTCTC AGCAAGAAAC
    CCTAAAGACC AGGAAGCATT GTGGGACATG GGAGCAGTCA GCATGCTCAA GAACCTCATT
    CATTCAAAGC ACAAGATGAT TGCTATGGGA AGTGCTGCAG CTTTAAGGAA TCTCATGGCA
    AATAGACCTG CAAAGTATAA AGATGCCAAT ATCATGTCTC CTGGTTCAAG TTTGCCTTCT
    CTACATGTCA GGAAACAAAA GGCCCTGGAA GCAGAATTAG ATGCTCAGCA TTTATCAGAA
    ACTTTTGACA ATATTGACAA TTTAAGTCCC AAGGCATCTC ATCGTAGCAA GCAGAGACAC
    AAGCAAAATC TCTATGGTGA CTATGTTTTT GACACCAATC GACATGATGA TAACAGGTCA
    GACAATTTTA ATACTGGAAA CATGACTGTC CTATCACCGT ACTTAAACAC TACAGTATTG
    CCCAGCTCTT CTTCATCAAG GGGAAGTTTA GATAGCTCTC GTTCTGAGAA AGATAGAAGT
    CTGGAGAGAG AACGAGGTAT TAGCCTAGGC AACTATCACC CAGCAACAGA AAACCCAGGA
    ACCTCTTCAA AGCGAGGTTT GCAGATTTCT ACCACTGCAG CCCAGATTGC CAAAGTCATG
    GAAGAAGTAT CAGCTATTCA TACCTCTCAG GAAGACAGAA GTTCTGGGTC TACCACAGAA
    CTACACTGTG GGACAGATGA GAGGAATGCA CTAAGAAGAA GCTCTACCAC CCACACACAT
    GCAAACACGT ACAACTTTAC CAAGTCAGAA AACTCAAACA GAACATGTCC AATACCATAT
    GCCAAAGTAG AATATAAGAG ATCTTCAAAT GATAGTTTAA ATAGTGTCAG CAGTAGTGAT
    GGTTATGGTA AAAGAGGTCA GATGAAACCT TCAATTGAAT CCTATTCTGA AGATGATGAA
    AGTAAATTTT GCAGCTATGG TCAGTATCCA GCTGACCTAG CCCATAAAAT ACATAGTGCA
    AATCATATGG ATGATAATGA TGGAGAACTA GATACACCAA TAAATTACAG TCTCAAATAT
    TCCGATGAAC AGTTGAACTC TGGGAGGCAG AGCCCTTCAC AGAATGAAAG ATGGGCAAGA
    CCCAAACATA TACTAGAAGA TGAAATAAAA CCGAATGAGC AGAGACAATC AAGGAGTCAA
    AGCACAGCTT ATCCCGTGTA TCCTGAGAGC ACTGATGATA AACACCTCAA GTTCCAACCA
    CACTTTGGGC AGCAAGAATG TGTTTCCCCA TACAGGTCAA GAGCAGCCAA TGGATCTGAA
    ACAAATCGAG TAGGCTCTAA TCATGGAATT AGTCAAAATG TGAACCAGTC TTTGTGTCAA
    GAAGATGACT ATGAAGACGA TAAACCAACC AACTATAGCG AACGTTACTC TGAGGAAGGG
    CAGCATGAGG AAGAAGAGAG GCCAACCAAT TATAGCATAA AATACAGTGA AGAAAAACAT
    CACGTGGATC AGCCTATTGA TTATAGTTTA AAATACACCA CAGACATTCC TTCTTCACAG
    AAACCAGCAT TTTCATTCTC AAAGAATTCA TCTGGACAGA GCACAAAAAC TGAACACATC
    TCTTCAAGCA GCGAGAATAC ATCCACAACT TCATCTAATG CCAAGAGGCA GAATCAGCTG
    CATCCAAGCT CAGCACAGAG CAGAAGTGGT CAGACCCCAA AAGCCACCTC TTCCTCTTGC
    AAAGTCCCCT CTATCAACCA AGAAACAATA CAGACTTACT GTGTAGAAGA TACCCCAATA
    TGTTTTTCAA GATGCAGTTC ATTATCATCT CTGTCATCTG CTGAAGATGA AGTAGGGTGT
    GATCAGACAA CACAAGAAGC AGAGTCTGCT AACACTCTGC AAATAGCTGA AATCAAGGAC
    AACAGCGGAC CTAGGTCAAA TGAAGATTCT GTGAGTAAAG TTCCAGCAGG GTCACAGCAC
    ATTAGAACCA AATCCAGCAG ACTCCAGGCT TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC
    AAAGCTGTTG AATTTTCTTC AGGGGCCAAA TCTCCTTCCA AGAGCGGTGC TCAGACACCC
    AAAAGTCCAC CAGAGCACTA CGTTCAGGAG ACCCCACTCA TGTTTAGCAG ATGTACTTCA
    GTCAGTTCAC TCGACAGTTT TGAGAGTCGC TCGATTGCCA GCTCTGTTCA GAGTGAACCC
    TGCAGTGGAA TGGTAAGTGG CATCATAAGC CCCAGTGACC TCCCAGATAG CCCTGGACAA
    ACCATGCCAC CAAGCAGAAG CAAAACCCCT CCGCCGCCGC CTCCGCCTCC TCCTCAGACA
    GTTCAAACGA AGCAGGAAGT ACCTAAAAAT AAAGCACCTA GTGCTGAAAA GAGAGAAAGT
    GGACCCAAGC AAGCTGCTGT AAATGCTGCA GTACAAAGGG TCCAGGTTCT TCCAGAGGCT
    GATACTCTGT TACATTTTGC CACAGAGAGT ACTCCTGATG GATTTTCTTG TTCATCTAGC
    CTGAGCGCTC TGAGCCTTGA TGAGCCATTT ATTCAGAAAG ATGTGGAACT AAGAATAATG
    CCTCCAGTTC AGGAAAATGA CAACGGGAAT GAAACAGAAA GTGAGCAGCC TGAAGAATCA
    AATGAAAACC AGGAAAAAGA GGCAGAAAAA CCCACTGATT CTGAAAAAGA TCTTTTAGAT
    GAGTCAGATG ATGATGATAT TGAAATACTA GAAGAGTGTA TAATTTCTGC CATGCCAACA
    AAATCTTCAC GCAAAGCCAA AAAACCAGCC CAGACTACTT CAAAATTACC TCCACCTGTG
    GCAAGGAAAC CAAGTCAGCT CCCTGTATAC AAACTTCTGC CATCGCAAAA CAGGTTACAG
    GCACAAAAGC ATGTTAGTTT TACACCAGGA GATGATATGC CACGGGTGTA TTGTGTAGAA
    GGGACACCTA TAAACTTTTC CACAGCTACA TCTCTGAGTG ATCTAACGAT AGAATCCCCT
    CCAAATGAGT TAGCTGCTGG AGAAGGGGTT AGAGCAGGGG CACAGTCAAG TGAGTTTGAA
    AAACGAGATA CCATTCCTAC TGAAGGCAGA AGTACAGATG AGGCTCAACG AGGGAAAGCC
    TCATCTGTCA CTGTGCCTGA ACTGGACGAC AGCAAAACAG AAGAAGGTGA TATTCTTGCA
    GAATGCATTA ATTCTGCCAT GCCCAAAGGA AAAAGTCACA AGCCTTTCCG TGTGAAAAAG
    ATCATGGACC AGGTCCAGCA AGCATCTATG TCTTCATCTG GAACTAACAA AAATCAATTA
    GATGGTAAGA CGAAGAAACC TACTTCACCA GTAAAACCTA TACCACAAAA TACTGAATAC
    AGGACACGTG TAAGAAAAAA TACAGACTCA AAAAATAATC TAAATGCAGA AAGAAATTTC
    TCGGAAAACA AAGACTCAAA GAAACAGCAC TTGAAAAATA ATTCCAAGGA CTTCAATGAT
    AAACTGCCAA ATAATGAAGA CAGAGTCAGA GGAAGTTTTA CTTTTGATTC ACCCCATCAT
    TACACGCCCA TTGAAGGCAC TCCGTACTGT TTTTCACGAA ATGACTCTCT GAGTTCTCTA
    GATTTTGATG ATGATGATGT TGACCTTTCC AGGGAAAAGG CTGAATTAAG AAAGGGGAAG
    GAAAATAAAG AATCAGAAGC TAAAGTGACC AACCACACAG AACTAACCTC AAACCAACAA
    TCAGCTAGTA AGACACCAGC TGTTACAAAG CAGCCAATAA ATAGAGGTCA GTCTAAACCC
    GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG TCTTCCAAAG ATATACCAGA CAGAGGGGCA
    GCAACAGATG AGAAATTACA GAATTTCGCT ATTGAAAATA CTCCAGTTTG CTTTTCTCGA
    AATTCCTCTC TAAGCTCTCT TAGTGACATT GATCAAGAAA ACAACAACAA CAAGGAAAAT
    GAACCTGTCA AAGAGACAGA GCCCCCTGCC TCACAGGGAG AACCAGGTAA ACCCCAGGCC
    TCAGGTTATG CTCCTAAATC GTTTCACGTG GAAGACACCC CTGTTTGTTT CTCAAGAAAC
    AGTTCTCTCA GTTCTCTTAG TATTGATTCT GAAGATGACC TGCTGCAGGA ATGTATAAGT
    TCTGCAATGC CAAAAAAGAA AAAGCCTTCA AGGCTCAAGC CCGATAATGA AAAGCATAGT
    CCCAGAAATA TGGGTGGCAT ATTAGCAGAA GATTTGACAC TCGATTTGAA AGATATACAG
    AGACCAGATT CAGAACATGG TTTATCCCCA GATTCAGAAA ATTTTGATTG GAAAGCTATT
    CAGGAAGGTG CAAATTCCAT AGTAAGTAGT TTACATCAAG CTGCTGCCGC TGCATGTTTA
    TCTCGACAAG CTTCATCTGA TTCAGATTCT ATCCTTTCAT TGAAATCGGG TATCTCTCTG
    GGATCACCAT TTCACCTTAC ACCTGATCAA GAAGAAAAAC CCTTTACGAG TAATAAAGGT
    CCACGAATTC TAAAACCTGG GGAGAAAAGT ACATTGGAAA CTAAAAAAAT AGAATCTGAA
    AATAAAGGAA TAAAAGGAGG CAAAAAGGTT TATAAAAGTT TGATTACTGG AAAAGTTCGA
    TCTAATTCGG AAATTTCGAG CCAAATGAAA CAACCCCTTC AAACAAACAT GCCTTCAATC
    TCTCGAGGTA GGACAATGAT TCATATTCCA GGAGTTCGGA ATAGCTCTTC AAGTACAAGC
    CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT
    CAGCCGGCTA CCACCTCTCC CAGAGGAACC AAGCCATCAG TGAAGTCAGA ATTAAGCCCT
    GTTACAAGGC AGGCATCCCA GACAGCAGGA TCAAACAAAG GACCTTCTAG ATCAGGATCT
    AGAGATTCCA CTCCTTCAAG ACCTGCCCAG CAACCATTAA GTAGACCAAT GCAGTCTCCA
    GGGCGAAACT CAATCTCTCC TGGTAGAAAT GGAATAAGTC CCCCTAACAA ATTATCTCAA
    CTACCAAGGA CGTCATCCCC TAGTACTGCT TCAACTAAGT CCTCAGGTTC TGGGAAAATG
    TCTTACACAT CTCCTGGCAG ACAGATGAGC CAGCAGAACC TCACCAAACA AACAGGCTTA
    TCCAAGAATG GCAGTGGTAT CCCAAGAAGT GAATCTGCCT CCAAAGGGCT AAATCAAATG
    AGTAATAGTA ATGGATCCAA TAAAAAAGTA GAACTTTCTA GAATGTCTTC AACAAAGTCA
    AGTGGAAGTG AATCCGATAG GTCAGAGAGA CCTGTATTAG TACGCCAATC AACTTTCATC
    AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA AAACTGGAGG AATCTGCTTC ATTTGAATCT
    CTTTCTCCAT CTTCCAGACC CGATTCTCCC ACACGGTCCC AGGCTCATAC TCCAGTTTTA
    AGTCCTTCCC TTCCTGATAT GTCTCTATCT ACACATTCGT CTCTTCAGTC TGGTGGATGG
    CGAAAACTCC CACCTAACCT CAGTCCCACC ATAGAGTATA ATGATGGAAG ACCAGTAAAG
    CGCCATGATA TAGCACGCTC TCATTCCGAA AGTCCTTCCA GACTTCCCAT CAATAGGTCA
    GGGACCTGGA AACGTGAGCA CAGCAAACAC TCATCATCAC TTCCTCGAGT AAGCACTTGG
    AGAAGAACTG GAAGTTCATC CTCAATTCTT TCTGCTTCAT CAGAATCTAG TGAAAAGGCA
    AAAAGTGAGG ACGAAAAACA AGTGAACTCT ATTTCAGGAA GCAAACAAAC TAAAGAAAAC
    CAGGTATCCA CAAAAGGAAC ATGGAGAAAA ATAAAAGAAA GTGAAATTTC TCCCACAAAT
    AGTACTTCTC AGACCACTTC TTCAGGTGCT GCAAATGGTG CTGAATCAAA GACTCTGATT
    TATCAAATGG CACCTGCTGT TTCTAAAACA GAGGATGTTT GGGTGAGAAT TGAGGATTGC
    CCCATTAACA ACCCTAGATC TGGAAGATCT CCAACAGGAA ATACTCCCCC CGTGATTGAC
    ACTGTTTCAG AAAAGGGAAA CCCGAACCCT AAAGATTCAA AAGATAATCA GGGAAAACAA
    AATGTGAGCA ATGGTAGTGC CCCTACACGC ACCATGGGTC TGGAAAACCG CCTGAATTCC
    TTTATTCAGG TAGATCCTCC AGACCAAAAA GGAACTGAGA CAAAACCGGG ACACAGTAAT
    AACCCTGTCC CTGCATCCGA GACTAGTGAA AGTTCTATAG CCGAGCGTAC CCCGTTTAGT
    TCTAGCAGCT CAAGCAAGCA CAGTTCACCA AGTGGGACTG TCGCCGCCAG GGTGAGTCCT
    TTTAATTACA ACCCAAGCCC AAGGAAGAGC AGCACAGATG GCACTTCAGC CCGACCGTCT
    CAGATCCCAA CGCCAGTGAG CAACAACACA AAGAAACGCG ACTCAAAACC CGACAGCACA
    GAACCCAGCG GGACTCAAAG TCCTAAACGC CATTCTGGGT CTTACCTTGT GACATCTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211259.1
    CDS142..8424
    Translation

    Target ORF information:

    RefSeq Version XM_005211202.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211202.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGATACGAG CTTACTGTGA AACCTGTTGG GAGTGGCAGG AAGCCCATGA ACAAGGGATG
    GACCAGGACA AAAATCCAAT GCCAGCTCCT GTTGAACATC AGATCTGCCC TGCTGTCTGT
    GTTCTAATGA AGCTTTCGTT TGATGAAGAG CATAGACATG CCATGAATGA GCTTGGGGGA
    CTACAGGCCA TTGCAGAATT ATTGCAAGTG GACTGTGAAA TGTATGGGCT TACTAATGAC
    CACTACAGTA TTACTTTAAG ACGATATGCA GGAATGGCTT TGACAAACTT GACTTTCGGA
    GATGTAGCCA ACAAGGCTAC TCTCTGCTCT ATGAAAGGCT GCATGAGAGC GCTCGTGGCT
    CAGCTCCAGT CTGAAAGCGA GGACTTGCAG CAGGTTATTG CGAGTGTTTT GAGGAATCTG
    TCTTGGAGAG CAGATGTAAA TAGTAAAAAG ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA
    TTGATGGAAT GTGCTTTGGA AGTGAAAAAG GAATCAACCC TCAAAAGTGT ATTGAGTGCC
    TTATGGAACT TGTCAGCACA CTGCACTGAG AATAAAGCTG ACATATGTGC TGTGGATGGT
    GCGCTTGCAT TTTTGGTTGG CACTCTCACT TATCGCAGCC AGACCAATAC TTTAGCCATT
    ATTGAAAGTG GAGGTGGTAT ATTACGGAAT GTATCCAGCT TGATAGCTAC AAATGAGGAC
    CACAGGCAAA TCCTAAGAGA GAATAATTGC TTACAGACCT TATTACAACA CTTGAAATCT
    CACAGTTTGA CAATAGTCAG CAATGCATGC GGAACCTTGT GGAATCTCTC AGCAAGAAAC
    CCTAAAGACC AGGAAGCATT GTGGGACATG GGAGCAGTCA GCATGCTCAA GAACCTCATT
    CATTCAAAGC ACAAGATGAT TGCTATGGGA AGTGCTGCAG CTTTAAGGAA TCTCATGGCA
    AATAGACCTG CAAAGTATAA AGATGCCAAT ATCATGTCTC CTGGTTCAAG TTTGCCTTCT
    CTACATGTCA GGAAACAAAA GGCCCTGGAA GCAGAATTAG ATGCTCAGCA TTTATCAGAA
    ACTTTTGACA ATATTGACAA TTTAAGTCCC AAGGCATCTC ATCGTAGCAA GCAGAGACAC
    AAGCAAAATC TCTATGGTGA CTATGTTTTT GACACCAATC GACATGATGA TAACAGGTCA
    GACAATTTTA ATACTGGAAA CATGACTGTC CTATCACCGT ACTTAAACAC TACAGTATTG
    CCCAGCTCTT CTTCATCAAG GGGAAGTTTA GATAGCTCTC GTTCTGAGAA AGATAGAAGT
    CTGGAGAGAG AACGAGGTAT TAGCCTAGGC AACTATCACC CAGCAACAGA AAACCCAGGA
    ACCTCTTCAA AGCGAGGTTT GCAGATTTCT ACCACTGCAG CCCAGATTGC CAAAGTCATG
    GAAGAAGTAT CAGCTATTCA TACCTCTCAG GAAGACAGAA GTTCTGGGTC TACCACAGAA
    CTACACTGTG GGACAGATGA GAGGAATGCA CTAAGAAGAA GCTCTACCAC CCACACACAT
    GCAAACACGT ACAACTTTAC CAAGTCAGAA AACTCAAACA GAACATGTCC AATACCATAT
    GCCAAAGTAG AATATAAGAG ATCTTCAAAT GATAGTTTAA ATAGTGTCAG CAGTAGTGAT
    GGTTATGGTA AAAGAGGTCA GATGAAACCT TCAATTGAAT CCTATTCTGA AGATGATGAA
    AGTAAATTTT GCAGCTATGG TCAGTATCCA GCTGACCTAG CCCATAAAAT ACATAGTGCA
    AATCATATGG ATGATAATGA TGGAGAACTA GATACACCAA TAAATTACAG TCTCAAATAT
    TCCGATGAAC AGTTGAACTC TGGGAGGCAG AGCCCTTCAC AGAATGAAAG ATGGGCAAGA
    CCCAAACATA TACTAGAAGA TGAAATAAAA CCGAATGAGC AGAGACAATC AAGGAGTCAA
    AGCACAGCTT ATCCCGTGTA TCCTGAGAGC ACTGATGATA AACACCTCAA GTTCCAACCA
    CACTTTGGGC AGCAAGAATG TGTTTCCCCA TACAGGTCAA GAGCAGCCAA TGGATCTGAA
    ACAAATCGAG TAGGCTCTAA TCATGGAATT AGTCAAAATG TGAACCAGTC TTTGTGTCAA
    GAAGATGACT ATGAAGACGA TAAACCAACC AACTATAGCG AACGTTACTC TGAGGAAGGG
    CAGCATGAGG AAGAAGAGAG GCCAACCAAT TATAGCATAA AATACAGTGA AGAAAAACAT
    CACGTGGATC AGCCTATTGA TTATAGTTTA AAATACACCA CAGACATTCC TTCTTCACAG
    AAACCAGCAT TTTCATTCTC AAAGAATTCA TCTGGACAGA GCACAAAAAC TGAACACATC
    TCTTCAAGCA GCGAGAATAC ATCCACAACT TCATCTAATG CCAAGAGGCA GAATCAGCTG
    CATCCAAGCT CAGCACAGAG CAGAAGTGGT CAGACCCCAA AAGCCACCTC TTCCTCTTGC
    AAAGTCCCCT CTATCAACCA AGAAACAATA CAGACTTACT GTGTAGAAGA TACCCCAATA
    TGTTTTTCAA GATGCAGTTC ATTATCATCT CTGTCATCTG CTGAAGATGA AGTAGGGTGT
    GATCAGACAA CACAAGAAGC AGAGTCTGCT AACACTCTGC AAATAGCTGA AATCAAGGAC
    AACAGCGGAC CTAGGTCAAA TGAAGATTCT GTGAGTAAAG TTCCAGCAGG GTCACAGCAC
    ATTAGAACCA AATCCAGCAG ACTCCAGGCT TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC
    AAAGCTGTTG AATTTTCTTC AGGGGCCAAA TCTCCTTCCA AGAGCGGTGC TCAGACACCC
    AAAAGTCCAC CAGAGCACTA CGTTCAGGAG ACCCCACTCA TGTTTAGCAG ATGTACTTCA
    GTCAGTTCAC TCGACAGTTT TGAGAGTCGC TCGATTGCCA GCTCTGTTCA GAGTGAACCC
    TGCAGTGGAA TGGTAAGTGG CATCATAAGC CCCAGTGACC TCCCAGATAG CCCTGGACAA
    ACCATGCCAC CAAGCAGAAG CAAAACCCCT CCGCCGCCGC CTCCGCCTCC TCCTCAGACA
    GTTCAAACGA AGCAGGAAGT ACCTAAAAAT AAAGCACCTA GTGCTGAAAA GAGAGAAAGT
    GGACCCAAGC AAGCTGCTGT AAATGCTGCA GTACAAAGGG TCCAGGTTCT TCCAGAGGCT
    GATACTCTGT TACATTTTGC CACAGAGAGT ACTCCTGATG GATTTTCTTG TTCATCTAGC
    CTGAGCGCTC TGAGCCTTGA TGAGCCATTT ATTCAGAAAG ATGTGGAACT AAGAATAATG
    CCTCCAGTTC AGGAAAATGA CAACGGGAAT GAAACAGAAA GTGAGCAGCC TGAAGAATCA
    AATGAAAACC AGGAAAAAGA GGCAGAAAAA CCCACTGATT CTGAAAAAGA TCTTTTAGAT
    GAGTCAGATG ATGATGATAT TGAAATACTA GAAGAGTGTA TAATTTCTGC CATGCCAACA
    AAATCTTCAC GCAAAGCCAA AAAACCAGCC CAGACTACTT CAAAATTACC TCCACCTGTG
    GCAAGGAAAC CAAGTCAGCT CCCTGTATAC AAACTTCTGC CATCGCAAAA CAGGTTACAG
    GCACAAAAGC ATGTTAGTTT TACACCAGGA GATGATATGC CACGGGTGTA TTGTGTAGAA
    GGGACACCTA TAAACTTTTC CACAGCTACA TCTCTGAGTG ATCTAACGAT AGAATCCCCT
    CCAAATGAGT TAGCTGCTGG AGAAGGGGTT AGAGCAGGGG CACAGTCAAG TGAGTTTGAA
    AAACGAGATA CCATTCCTAC TGAAGGCAGA AGTACAGATG AGGCTCAACG AGGGAAAGCC
    TCATCTGTCA CTGTGCCTGA ACTGGACGAC AGCAAAACAG AAGAAGGTGA TATTCTTGCA
    GAATGCATTA ATTCTGCCAT GCCCAAAGGA AAAAGTCACA AGCCTTTCCG TGTGAAAAAG
    ATCATGGACC AGGTCCAGCA AGCATCTATG TCTTCATCTG GAACTAACAA AAATCAATTA
    GATGGTAAGA CGAAGAAACC TACTTCACCA GTAAAACCTA TACCACAAAA TACTGAATAC
    AGGACACGTG TAAGAAAAAA TACAGACTCA AAAAATAATC TAAATGCAGA AAGAAATTTC
    TCGGAAAACA AAGACTCAAA GAAACAGCAC TTGAAAAATA ATTCCAAGGA CTTCAATGAT
    AAACTGCCAA ATAATGAAGA CAGAGTCAGA GGAAGTTTTA CTTTTGATTC ACCCCATCAT
    TACACGCCCA TTGAAGGCAC TCCGTACTGT TTTTCACGAA ATGACTCTCT GAGTTCTCTA
    GATTTTGATG ATGATGATGT TGACCTTTCC AGGGAAAAGG CTGAATTAAG AAAGGGGAAG
    GAAAATAAAG AATCAGAAGC TAAAGTGACC AACCACACAG AACTAACCTC AAACCAACAA
    TCAGCTAGTA AGACACCAGC TGTTACAAAG CAGCCAATAA ATAGAGGTCA GTCTAAACCC
    GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG TCTTCCAAAG ATATACCAGA CAGAGGGGCA
    GCAACAGATG AGAAATTACA GAATTTCGCT ATTGAAAATA CTCCAGTTTG CTTTTCTCGA
    AATTCCTCTC TAAGCTCTCT TAGTGACATT GATCAAGAAA ACAACAACAA CAAGGAAAAT
    GAACCTGTCA AAGAGACAGA GCCCCCTGCC TCACAGGGAG AACCAGGTAA ACCCCAGGCC
    TCAGGTTATG CTCCTAAATC GTTTCACGTG GAAGACACCC CTGTTTGTTT CTCAAGAAAC
    AGTTCTCTCA GTTCTCTTAG TATTGATTCT GAAGATGACC TGCTGCAGGA ATGTATAAGT
    TCTGCAATGC CAAAAAAGAA AAAGCCTTCA AGGCTCAAGC CCGATAATGA AAAGCATAGT
    CCCAGAAATA TGGGTGGCAT ATTAGCAGAA GATTTGACAC TCGATTTGAA AGATATACAG
    AGACCAGATT CAGAACATGG TTTATCCCCA GATTCAGAAA ATTTTGATTG GAAAGCTATT
    CAGGAAGGTG CAAATTCCAT AGTAAGTAGT TTACATCAAG CTGCTGCCGC TGCATGTTTA
    TCTCGACAAG CTTCATCTGA TTCAGATTCT ATCCTTTCAT TGAAATCGGG TATCTCTCTG
    GGATCACCAT TTCACCTTAC ACCTGATCAA GAAGAAAAAC CCTTTACGAG TAATAAAGGT
    CCACGAATTC TAAAACCTGG GGAGAAAAGT ACATTGGAAA CTAAAAAAAT AGAATCTGAA
    AATAAAGGAA TAAAAGGAGG CAAAAAGGTT TATAAAAGTT TGATTACTGG AAAAGTTCGA
    TCTAATTCGG AAATTTCGAG CCAAATGAAA CAACCCCTTC AAACAAACAT GCCTTCAATC
    TCTCGAGGTA GGACAATGAT TCATATTCCA GGAGTTCGGA ATAGCTCTTC AAGTACAAGC
    CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT
    CAGCCGGCTA CCACCTCTCC CAGAGGAACC AAGCCATCAG TGAAGTCAGA ATTAAGCCCT
    GTTACAAGGC AGGCATCCCA GACAGCAGGA TCAAACAAAG GACCTTCTAG ATCAGGATCT
    AGAGATTCCA CTCCTTCAAG ACCTGCCCAG CAACCATTAA GTAGACCAAT GCAGTCTCCA
    GGGCGAAACT CAATCTCTCC TGGTAGAAAT GGAATAAGTC CCCCTAACAA ATTATCTCAA
    CTACCAAGGA CGTCATCCCC TAGTACTGCT TCAACTAAGT CCTCAGGTTC TGGGAAAATG
    TCTTACACAT CTCCTGGCAG ACAGATGAGC CAGCAGAACC TCACCAAACA AACAGGCTTA
    TCCAAGAATG GCAGTGGTAT CCCAAGAAGT GAATCTGCCT CCAAAGGGCT AAATCAAATG
    AGTAATAGTA ATGGATCCAA TAAAAAAGTA GAACTTTCTA GAATGTCTTC AACAAAGTCA
    AGTGGAAGTG AATCCGATAG GTCAGAGAGA CCTGTATTAG TACGCCAATC AACTTTCATC
    AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA AAACTGGAGG AATCTGCTTC ATTTGAATCT
    CTTTCTCCAT CTTCCAGACC CGATTCTCCC ACACGGTCCC AGGCTCATAC TCCAGTTTTA
    AGTCCTTCCC TTCCTGATAT GTCTCTATCT ACACATTCGT CTCTTCAGTC TGGTGGATGG
    CGAAAACTCC CACCTAACCT CAGTCCCACC ATAGAGTATA ATGATGGAAG ACCAGTAAAG
    CGCCATGATA TAGCACGCTC TCATTCCGAA AGTCCTTCCA GACTTCCCAT CAATAGGTCA
    GGGACCTGGA AACGTGAGCA CAGCAAACAC TCATCATCAC TTCCTCGAGT AAGCACTTGG
    AGAAGAACTG GAAGTTCATC CTCAATTCTT TCTGCTTCAT CAGAATCTAG TGAAAAGGCA
    AAAAGTGAGG ACGAAAAACA AGTGAACTCT ATTTCAGGAA GCAAACAAAC TAAAGAAAAC
    CAGGTATCCA CAAAAGGAAC ATGGAGAAAA ATAAAAGAAA GTGAAATTTC TCCCACAAAT
    AGTACTTCTC AGACCACTTC TTCAGGTGCT GCAAATGGTG CTGAATCAAA GACTCTGATT
    TATCAAATGG CACCTGCTGT TTCTAAAACA GAGGATGTTT GGGTGAGAAT TGAGGATTGC
    CCCATTAACA ACCCTAGATC TGGAAGATCT CCAACAGGAA ATACTCCCCC CGTGATTGAC
    ACTGTTTCAG AAAAGGGAAA CCCGAACCCT AAAGATTCAA AAGATAATCA GGGAAAACAA
    AATGTGAGCA ATGGTAGTGC CCCTACACGC ACCATGGGTC TGGAAAACCG CCTGAATTCC
    TTTATTCAGG TAGATCCTCC AGACCAAAAA GGAACTGAGA CAAAACCGGG ACACAGTAAT
    AACCCTGTCC CTGCATCCGA GACTAGTGAA AGTTCTATAG CCGAGCGTAC CCCGTTTAGT
    TCTAGCAGCT CAAGCAAGCA CAGTTCACCA AGTGGGACTG TCGCCGCCAG GGTGAGTCCT
    TTTAATTACA ACCCAAGCCC AAGGAAGAGC AGCACAGATG GCACTTCAGC CCGACCGTCT
    CAGATCCCAA CGCCAGTGAG CAACAACACA AAGAAACGCG ACTCAAAACC CGACAGCACA
    GAACCCAGCG GGACTCAAAG TCCTAAACGC CATTCTGGGT CTTACCTTGT GACATCTGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22869
    Clone ID Related Accession (Same CDS sequence) XM_005211203.3
    Accession Version XM_005211203.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211203.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGATACGAG CTTACTGTGA AACCTGTTGG
    GAGTGGCAGG AAGCCCATGA ACAAGGGATG GACCAGGACA AAAATCCAAT GCCAGCTCCT
    GTTGAACATC AGATCTGCCC TGCTGTCTGT GTTCTAATGA AGCTTTCGTT TGATGAAGAG
    CATAGACATG CCATGAATGA GCTTGGGGGA CTACAGGCCA TTGCAGAATT ATTGCAAGTG
    GACTGTGAAA TGTATGGGCT TACTAATGAC CACTACAGTA TTACTTTAAG ACGATATGCA
    GGAATGGCTT TGACAAACTT GACTTTCGGA GATGTAGCCA ACAAGGCTAC TCTCTGCTCT
    ATGAAAGGCT GCATGAGAGC GCTCGTGGCT CAGCTCCAGT CTGAAAGCGA GGACTTGCAG
    CAGGTTATTG CGAGTGTTTT GAGGAATCTG TCTTGGAGAG CAGATGTAAA TAGTAAAAAG
    ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTGAAAAAG
    GAATCAACCC TCAAAAGTGT ATTGAGTGCC TTATGGAACT TGTCAGCACA CTGCACTGAG
    AATAAAGCTG ACATATGTGC TGTGGATGGT GCGCTTGCAT TTTTGGTTGG CACTCTCACT
    TATCGCAGCC AGACCAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGTAT ATTACGGAAT
    GTATCCAGCT TGATAGCTAC AAATGAGGAC CACAGGCAAA TCCTAAGAGA GAATAATTGC
    TTACAGACCT TATTACAACA CTTGAAATCT CACAGTTTGA CAATAGTCAG CAATGCATGC
    GGAACCTTGT GGAATCTCTC AGCAAGAAAC CCTAAAGACC AGGAAGCATT GTGGGACATG
    GGAGCAGTCA GCATGCTCAA GAACCTCATT CATTCAAAGC ACAAGATGAT TGCTATGGGA
    AGTGCTGCAG CTTTAAGGAA TCTCATGGCA AATAGACCTG CAAAGTATAA AGATGCCAAT
    ATCATGTCTC CTGGTTCAAG TTTGCCTTCT CTACATGTCA GGAAACAAAA GGCCCTGGAA
    GCAGAATTAG ATGCTCAGCA TTTATCAGAA ACTTTTGACA ATATTGACAA TTTAAGTCCC
    AAGGCATCTC ATCGTAGCAA GCAGAGACAC AAGCAAAATC TCTATGGTGA CTATGTTTTT
    GACACCAATC GACATGATGA TAACAGGTCA GACAATTTTA ATACTGGAAA CATGACTGTC
    CTATCACCGT ACTTAAACAC TACAGTATTG CCCAGCTCTT CTTCATCAAG GGGAAGTTTA
    GATAGCTCTC GTTCTGAGAA AGATAGAAGT CTGGAGAGAG AACGAGGTAT TAGCCTAGGC
    AACTATCACC CAGCAACAGA AAACCCAGGA ACCTCTTCAA AGCGAGGTTT GCAGATTTCT
    ACCACTGCAG CCCAGATTGC CAAAGTCATG GAAGAAGTAT CAGCTATTCA TACCTCTCAG
    GAAGACAGAA GTTCTGGGTC TACCACAGAA CTACACTGTG GGACAGATGA GAGGAATGCA
    CTAAGAAGAA GCTCTACCAC CCACACACAT GCAAACACGT ACAACTTTAC CAAGTCAGAA
    AACTCAAACA GAACATGTCC AATACCATAT GCCAAAGTAG AATATAAGAG ATCTTCAAAT
    GATAGTTTAA ATAGTGTCAG CAGTAGTGAT GGTTATGGTA AAAGAGGTCA GATGAAACCT
    TCAATTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGCTATGG TCAGTATCCA
    GCTGACCTAG CCCATAAAAT ACATAGTGCA AATCATATGG ATGATAATGA TGGAGAACTA
    GATACACCAA TAAATTACAG TCTCAAATAT TCCGATGAAC AGTTGAACTC TGGGAGGCAG
    AGCCCTTCAC AGAATGAAAG ATGGGCAAGA CCCAAACATA TACTAGAAGA TGAAATAAAA
    CCGAATGAGC AGAGACAATC AAGGAGTCAA AGCACAGCTT ATCCCGTGTA TCCTGAGAGC
    ACTGATGATA AACACCTCAA GTTCCAACCA CACTTTGGGC AGCAAGAATG TGTTTCCCCA
    TACAGGTCAA GAGCAGCCAA TGGATCTGAA ACAAATCGAG TAGGCTCTAA TCATGGAATT
    AGTCAAAATG TGAACCAGTC TTTGTGTCAA GAAGATGACT ATGAAGACGA TAAACCAACC
    AACTATAGCG AACGTTACTC TGAGGAAGGG CAGCATGAGG AAGAAGAGAG GCCAACCAAT
    TATAGCATAA AATACAGTGA AGAAAAACAT CACGTGGATC AGCCTATTGA TTATAGTTTA
    AAATACACCA CAGACATTCC TTCTTCACAG AAACCAGCAT TTTCATTCTC AAAGAATTCA
    TCTGGACAGA GCACAAAAAC TGAACACATC TCTTCAAGCA GCGAGAATAC ATCCACAACT
    TCATCTAATG CCAAGAGGCA GAATCAGCTG CATCCAAGCT CAGCACAGAG CAGAAGTGGT
    CAGACCCCAA AAGCCACCTC TTCCTCTTGC AAAGTCCCCT CTATCAACCA AGAAACAATA
    CAGACTTACT GTGTAGAAGA TACCCCAATA TGTTTTTCAA GATGCAGTTC ATTATCATCT
    CTGTCATCTG CTGAAGATGA AGTAGGGTGT GATCAGACAA CACAAGAAGC AGAGTCTGCT
    AACACTCTGC AAATAGCTGA AATCAAGGAC AACAGCGGAC CTAGGTCAAA TGAAGATTCT
    GTGAGTAAAG TTCCAGCAGG GTCACAGCAC ATTAGAACCA AATCCAGCAG ACTCCAGGCT
    TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC AAAGCTGTTG AATTTTCTTC AGGGGCCAAA
    TCTCCTTCCA AGAGCGGTGC TCAGACACCC AAAAGTCCAC CAGAGCACTA CGTTCAGGAG
    ACCCCACTCA TGTTTAGCAG ATGTACTTCA GTCAGTTCAC TCGACAGTTT TGAGAGTCGC
    TCGATTGCCA GCTCTGTTCA GAGTGAACCC TGCAGTGGAA TGGTAAGTGG CATCATAAGC
    CCCAGTGACC TCCCAGATAG CCCTGGACAA ACCATGCCAC CAAGCAGAAG CAAAACCCCT
    CCGCCGCCGC CTCCGCCTCC TCCTCAGACA GTTCAAACGA AGCAGGAAGT ACCTAAAAAT
    AAAGCACCTA GTGCTGAAAA GAGAGAAAGT GGACCCAAGC AAGCTGCTGT AAATGCTGCA
    GTACAAAGGG TCCAGGTTCT TCCAGAGGCT GATACTCTGT TACATTTTGC CACAGAGAGT
    ACTCCTGATG GATTTTCTTG TTCATCTAGC CTGAGCGCTC TGAGCCTTGA TGAGCCATTT
    ATTCAGAAAG ATGTGGAACT AAGAATAATG CCTCCAGTTC AGGAAAATGA CAACGGGAAT
    GAAACAGAAA GTGAGCAGCC TGAAGAATCA AATGAAAACC AGGAAAAAGA GGCAGAAAAA
    CCCACTGATT CTGAAAAAGA TCTTTTAGAT GAGTCAGATG ATGATGATAT TGAAATACTA
    GAAGAGTGTA TAATTTCTGC CATGCCAACA AAATCTTCAC GCAAAGCCAA AAAACCAGCC
    CAGACTACTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT CCCTGTATAC
    AAACTTCTGC CATCGCAAAA CAGGTTACAG GCACAAAAGC ATGTTAGTTT TACACCAGGA
    GATGATATGC CACGGGTGTA TTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCTACA
    TCTCTGAGTG ATCTAACGAT AGAATCCCCT CCAAATGAGT TAGCTGCTGG AGAAGGGGTT
    AGAGCAGGGG CACAGTCAAG TGAGTTTGAA AAACGAGATA CCATTCCTAC TGAAGGCAGA
    AGTACAGATG AGGCTCAACG AGGGAAAGCC TCATCTGTCA CTGTGCCTGA ACTGGACGAC
    AGCAAAACAG AAGAAGGTGA TATTCTTGCA GAATGCATTA ATTCTGCCAT GCCCAAAGGA
    AAAAGTCACA AGCCTTTCCG TGTGAAAAAG ATCATGGACC AGGTCCAGCA AGCATCTATG
    TCTTCATCTG GAACTAACAA AAATCAATTA GATGGTAAGA CGAAGAAACC TACTTCACCA
    GTAAAACCTA TACCACAAAA TACTGAATAC AGGACACGTG TAAGAAAAAA TACAGACTCA
    AAAAATAATC TAAATGCAGA AAGAAATTTC TCGGAAAACA AAGACTCAAA GAAACAGCAC
    TTGAAAAATA ATTCCAAGGA CTTCAATGAT AAACTGCCAA ATAATGAAGA CAGAGTCAGA
    GGAAGTTTTA CTTTTGATTC ACCCCATCAT TACACGCCCA TTGAAGGCAC TCCGTACTGT
    TTTTCACGAA ATGACTCTCT GAGTTCTCTA GATTTTGATG ATGATGATGT TGACCTTTCC
    AGGGAAAAGG CTGAATTAAG AAAGGGGAAG GAAAATAAAG AATCAGAAGC TAAAGTGACC
    AACCACACAG AACTAACCTC AAACCAACAA TCAGCTAGTA AGACACCAGC TGTTACAAAG
    CAGCCAATAA ATAGAGGTCA GTCTAAACCC GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG
    TCTTCCAAAG ATATACCAGA CAGAGGGGCA GCAACAGATG AGAAATTACA GAATTTCGCT
    ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TAAGCTCTCT TAGTGACATT
    GATCAAGAAA ACAACAACAA CAAGGAAAAT GAACCTGTCA AAGAGACAGA GCCCCCTGCC
    TCACAGGGAG AACCAGGTAA ACCCCAGGCC TCAGGTTATG CTCCTAAATC GTTTCACGTG
    GAAGACACCC CTGTTTGTTT CTCAAGAAAC AGTTCTCTCA GTTCTCTTAG TATTGATTCT
    GAAGATGACC TGCTGCAGGA ATGTATAAGT TCTGCAATGC CAAAAAAGAA AAAGCCTTCA
    AGGCTCAAGC CCGATAATGA AAAGCATAGT CCCAGAAATA TGGGTGGCAT ATTAGCAGAA
    GATTTGACAC TCGATTTGAA AGATATACAG AGACCAGATT CAGAACATGG TTTATCCCCA
    GATTCAGAAA ATTTTGATTG GAAAGCTATT CAGGAAGGTG CAAATTCCAT AGTAAGTAGT
    TTACATCAAG CTGCTGCCGC TGCATGTTTA TCTCGACAAG CTTCATCTGA TTCAGATTCT
    ATCCTTTCAT TGAAATCGGG TATCTCTCTG GGATCACCAT TTCACCTTAC ACCTGATCAA
    GAAGAAAAAC CCTTTACGAG TAATAAAGGT CCACGAATTC TAAAACCTGG GGAGAAAAGT
    ACATTGGAAA CTAAAAAAAT AGAATCTGAA AATAAAGGAA TAAAAGGAGG CAAAAAGGTT
    TATAAAAGTT TGATTACTGG AAAAGTTCGA TCTAATTCGG AAATTTCGAG CCAAATGAAA
    CAACCCCTTC AAACAAACAT GCCTTCAATC TCTCGAGGTA GGACAATGAT TCATATTCCA
    GGAGTTCGGA ATAGCTCTTC AAGTACAAGC CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG
    ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT CAGCCGGCTA CCACCTCTCC CAGAGGAACC
    AAGCCATCAG TGAAGTCAGA ATTAAGCCCT GTTACAAGGC AGGCATCCCA GACAGCAGGA
    TCAAACAAAG GACCTTCTAG ATCAGGATCT AGAGATTCCA CTCCTTCAAG ACCTGCCCAG
    CAACCATTAA GTAGACCAAT GCAGTCTCCA GGGCGAAACT CAATCTCTCC TGGTAGAAAT
    GGAATAAGTC CCCCTAACAA ATTATCTCAA CTACCAAGGA CGTCATCCCC TAGTACTGCT
    TCAACTAAGT CCTCAGGTTC TGGGAAAATG TCTTACACAT CTCCTGGCAG ACAGATGAGC
    CAGCAGAACC TCACCAAACA AACAGGCTTA TCCAAGAATG GCAGTGGTAT CCCAAGAAGT
    GAATCTGCCT CCAAAGGGCT AAATCAAATG AGTAATAGTA ATGGATCCAA TAAAAAAGTA
    GAACTTTCTA GAATGTCTTC AACAAAGTCA AGTGGAAGTG AATCCGATAG GTCAGAGAGA
    CCTGTATTAG TACGCCAATC AACTTTCATC AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA
    AAACTGGAGG AATCTGCTTC ATTTGAATCT CTTTCTCCAT CTTCCAGACC CGATTCTCCC
    ACACGGTCCC AGGCTCATAC TCCAGTTTTA AGTCCTTCCC TTCCTGATAT GTCTCTATCT
    ACACATTCGT CTCTTCAGTC TGGTGGATGG CGAAAACTCC CACCTAACCT CAGTCCCACC
    ATAGAGTATA ATGATGGAAG ACCAGTAAAG CGCCATGATA TAGCACGCTC TCATTCCGAA
    AGTCCTTCCA GACTTCCCAT CAATAGGTCA GGGACCTGGA AACGTGAGCA CAGCAAACAC
    TCATCATCAC TTCCTCGAGT AAGCACTTGG AGAAGAACTG GAAGTTCATC CTCAATTCTT
    TCTGCTTCAT CAGAATCTAG TGAAAAGGCA AAAAGTGAGG ACGAAAAACA AGTGAACTCT
    ATTTCAGGAA GCAAACAAAC TAAAGAAAAC CAGGTATCCA CAAAAGGAAC ATGGAGAAAA
    ATAAAAGAAA GTGAAATTTC TCCCACAAAT AGTACTTCTC AGACCACTTC TTCAGGTGCT
    GCAAATGGTG CTGAATCAAA GACTCTGATT TATCAAATGG CACCTGCTGT TTCTAAAACA
    GAGGATGTTT GGGTGAGAAT TGAGGATTGC CCCATTAACA ACCCTAGATC TGGAAGATCT
    CCAACAGGAA ATACTCCCCC CGTGATTGAC ACTGTTTCAG AAAAGGGAAA CCCGAACCCT
    AAAGATTCAA AAGATAATCA GGGAAAACAA AATGTGAGCA ATGGTAGTGC CCCTACACGC
    ACCATGGGTC TGGAAAACCG CCTGAATTCC TTTATTCAGG TAGATCCTCC AGACCAAAAA
    GGAACTGAGA CAAAACCGGG ACACAGTAAT AACCCTGTCC CTGCATCCGA GACTAGTGAA
    AGTTCTATAG CCGAGCGTAC CCCGTTTAGT TCTAGCAGCT CAAGCAAGCA CAGTTCACCA
    AGTGGGACTG TCGCCGCCAG GGTGAGTCCT TTTAATTACA ACCCAAGCCC AAGGAAGAGC
    AGCACAGATG GCACTTCAGC CCGACCGTCT CAGATCCCAA CGCCAGTGAG CAACAACACA
    AAGAAACGCG ACTCAAAACC CGACAGCACA GAACCCAGCG GGACTCAAAG TCCTAAACGC
    CATTCTGGGT CTTACCTTGT GACATCTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211260.1
    CDS121..8373
    Translation

    Target ORF information:

    RefSeq Version XM_005211203.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211203.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGGTTCA
    ACTACACGAA TAGATCATGA AACAGCCAGT GTTTTGAGTT CTAGTAGCAC ACATTCTGCT
    CCTCGAAGGC TGACGAGTCA TCTGGGAACC AAGATACGAG CTTACTGTGA AACCTGTTGG
    GAGTGGCAGG AAGCCCATGA ACAAGGGATG GACCAGGACA AAAATCCAAT GCCAGCTCCT
    GTTGAACATC AGATCTGCCC TGCTGTCTGT GTTCTAATGA AGCTTTCGTT TGATGAAGAG
    CATAGACATG CCATGAATGA GCTTGGGGGA CTACAGGCCA TTGCAGAATT ATTGCAAGTG
    GACTGTGAAA TGTATGGGCT TACTAATGAC CACTACAGTA TTACTTTAAG ACGATATGCA
    GGAATGGCTT TGACAAACTT GACTTTCGGA GATGTAGCCA ACAAGGCTAC TCTCTGCTCT
    ATGAAAGGCT GCATGAGAGC GCTCGTGGCT CAGCTCCAGT CTGAAAGCGA GGACTTGCAG
    CAGGTTATTG CGAGTGTTTT GAGGAATCTG TCTTGGAGAG CAGATGTAAA TAGTAAAAAG
    ACTTTGCGTG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTGAAAAAG
    GAATCAACCC TCAAAAGTGT ATTGAGTGCC TTATGGAACT TGTCAGCACA CTGCACTGAG
    AATAAAGCTG ACATATGTGC TGTGGATGGT GCGCTTGCAT TTTTGGTTGG CACTCTCACT
    TATCGCAGCC AGACCAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGTAT ATTACGGAAT
    GTATCCAGCT TGATAGCTAC AAATGAGGAC CACAGGCAAA TCCTAAGAGA GAATAATTGC
    TTACAGACCT TATTACAACA CTTGAAATCT CACAGTTTGA CAATAGTCAG CAATGCATGC
    GGAACCTTGT GGAATCTCTC AGCAAGAAAC CCTAAAGACC AGGAAGCATT GTGGGACATG
    GGAGCAGTCA GCATGCTCAA GAACCTCATT CATTCAAAGC ACAAGATGAT TGCTATGGGA
    AGTGCTGCAG CTTTAAGGAA TCTCATGGCA AATAGACCTG CAAAGTATAA AGATGCCAAT
    ATCATGTCTC CTGGTTCAAG TTTGCCTTCT CTACATGTCA GGAAACAAAA GGCCCTGGAA
    GCAGAATTAG ATGCTCAGCA TTTATCAGAA ACTTTTGACA ATATTGACAA TTTAAGTCCC
    AAGGCATCTC ATCGTAGCAA GCAGAGACAC AAGCAAAATC TCTATGGTGA CTATGTTTTT
    GACACCAATC GACATGATGA TAACAGGTCA GACAATTTTA ATACTGGAAA CATGACTGTC
    CTATCACCGT ACTTAAACAC TACAGTATTG CCCAGCTCTT CTTCATCAAG GGGAAGTTTA
    GATAGCTCTC GTTCTGAGAA AGATAGAAGT CTGGAGAGAG AACGAGGTAT TAGCCTAGGC
    AACTATCACC CAGCAACAGA AAACCCAGGA ACCTCTTCAA AGCGAGGTTT GCAGATTTCT
    ACCACTGCAG CCCAGATTGC CAAAGTCATG GAAGAAGTAT CAGCTATTCA TACCTCTCAG
    GAAGACAGAA GTTCTGGGTC TACCACAGAA CTACACTGTG GGACAGATGA GAGGAATGCA
    CTAAGAAGAA GCTCTACCAC CCACACACAT GCAAACACGT ACAACTTTAC CAAGTCAGAA
    AACTCAAACA GAACATGTCC AATACCATAT GCCAAAGTAG AATATAAGAG ATCTTCAAAT
    GATAGTTTAA ATAGTGTCAG CAGTAGTGAT GGTTATGGTA AAAGAGGTCA GATGAAACCT
    TCAATTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGCTATGG TCAGTATCCA
    GCTGACCTAG CCCATAAAAT ACATAGTGCA AATCATATGG ATGATAATGA TGGAGAACTA
    GATACACCAA TAAATTACAG TCTCAAATAT TCCGATGAAC AGTTGAACTC TGGGAGGCAG
    AGCCCTTCAC AGAATGAAAG ATGGGCAAGA CCCAAACATA TACTAGAAGA TGAAATAAAA
    CCGAATGAGC AGAGACAATC AAGGAGTCAA AGCACAGCTT ATCCCGTGTA TCCTGAGAGC
    ACTGATGATA AACACCTCAA GTTCCAACCA CACTTTGGGC AGCAAGAATG TGTTTCCCCA
    TACAGGTCAA GAGCAGCCAA TGGATCTGAA ACAAATCGAG TAGGCTCTAA TCATGGAATT
    AGTCAAAATG TGAACCAGTC TTTGTGTCAA GAAGATGACT ATGAAGACGA TAAACCAACC
    AACTATAGCG AACGTTACTC TGAGGAAGGG CAGCATGAGG AAGAAGAGAG GCCAACCAAT
    TATAGCATAA AATACAGTGA AGAAAAACAT CACGTGGATC AGCCTATTGA TTATAGTTTA
    AAATACACCA CAGACATTCC TTCTTCACAG AAACCAGCAT TTTCATTCTC AAAGAATTCA
    TCTGGACAGA GCACAAAAAC TGAACACATC TCTTCAAGCA GCGAGAATAC ATCCACAACT
    TCATCTAATG CCAAGAGGCA GAATCAGCTG CATCCAAGCT CAGCACAGAG CAGAAGTGGT
    CAGACCCCAA AAGCCACCTC TTCCTCTTGC AAAGTCCCCT CTATCAACCA AGAAACAATA
    CAGACTTACT GTGTAGAAGA TACCCCAATA TGTTTTTCAA GATGCAGTTC ATTATCATCT
    CTGTCATCTG CTGAAGATGA AGTAGGGTGT GATCAGACAA CACAAGAAGC AGAGTCTGCT
    AACACTCTGC AAATAGCTGA AATCAAGGAC AACAGCGGAC CTAGGTCAAA TGAAGATTCT
    GTGAGTAAAG TTCCAGCAGG GTCACAGCAC ATTAGAACCA AATCCAGCAG ACTCCAGGCT
    TCTGGTCTGT CTTCAGAGTC AGCCAGGCAC AAAGCTGTTG AATTTTCTTC AGGGGCCAAA
    TCTCCTTCCA AGAGCGGTGC TCAGACACCC AAAAGTCCAC CAGAGCACTA CGTTCAGGAG
    ACCCCACTCA TGTTTAGCAG ATGTACTTCA GTCAGTTCAC TCGACAGTTT TGAGAGTCGC
    TCGATTGCCA GCTCTGTTCA GAGTGAACCC TGCAGTGGAA TGGTAAGTGG CATCATAAGC
    CCCAGTGACC TCCCAGATAG CCCTGGACAA ACCATGCCAC CAAGCAGAAG CAAAACCCCT
    CCGCCGCCGC CTCCGCCTCC TCCTCAGACA GTTCAAACGA AGCAGGAAGT ACCTAAAAAT
    AAAGCACCTA GTGCTGAAAA GAGAGAAAGT GGACCCAAGC AAGCTGCTGT AAATGCTGCA
    GTACAAAGGG TCCAGGTTCT TCCAGAGGCT GATACTCTGT TACATTTTGC CACAGAGAGT
    ACTCCTGATG GATTTTCTTG TTCATCTAGC CTGAGCGCTC TGAGCCTTGA TGAGCCATTT
    ATTCAGAAAG ATGTGGAACT AAGAATAATG CCTCCAGTTC AGGAAAATGA CAACGGGAAT
    GAAACAGAAA GTGAGCAGCC TGAAGAATCA AATGAAAACC AGGAAAAAGA GGCAGAAAAA
    CCCACTGATT CTGAAAAAGA TCTTTTAGAT GAGTCAGATG ATGATGATAT TGAAATACTA
    GAAGAGTGTA TAATTTCTGC CATGCCAACA AAATCTTCAC GCAAAGCCAA AAAACCAGCC
    CAGACTACTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT CCCTGTATAC
    AAACTTCTGC CATCGCAAAA CAGGTTACAG GCACAAAAGC ATGTTAGTTT TACACCAGGA
    GATGATATGC CACGGGTGTA TTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCTACA
    TCTCTGAGTG ATCTAACGAT AGAATCCCCT CCAAATGAGT TAGCTGCTGG AGAAGGGGTT
    AGAGCAGGGG CACAGTCAAG TGAGTTTGAA AAACGAGATA CCATTCCTAC TGAAGGCAGA
    AGTACAGATG AGGCTCAACG AGGGAAAGCC TCATCTGTCA CTGTGCCTGA ACTGGACGAC
    AGCAAAACAG AAGAAGGTGA TATTCTTGCA GAATGCATTA ATTCTGCCAT GCCCAAAGGA
    AAAAGTCACA AGCCTTTCCG TGTGAAAAAG ATCATGGACC AGGTCCAGCA AGCATCTATG
    TCTTCATCTG GAACTAACAA AAATCAATTA GATGGTAAGA CGAAGAAACC TACTTCACCA
    GTAAAACCTA TACCACAAAA TACTGAATAC AGGACACGTG TAAGAAAAAA TACAGACTCA
    AAAAATAATC TAAATGCAGA AAGAAATTTC TCGGAAAACA AAGACTCAAA GAAACAGCAC
    TTGAAAAATA ATTCCAAGGA CTTCAATGAT AAACTGCCAA ATAATGAAGA CAGAGTCAGA
    GGAAGTTTTA CTTTTGATTC ACCCCATCAT TACACGCCCA TTGAAGGCAC TCCGTACTGT
    TTTTCACGAA ATGACTCTCT GAGTTCTCTA GATTTTGATG ATGATGATGT TGACCTTTCC
    AGGGAAAAGG CTGAATTAAG AAAGGGGAAG GAAAATAAAG AATCAGAAGC TAAAGTGACC
    AACCACACAG AACTAACCTC AAACCAACAA TCAGCTAGTA AGACACCAGC TGTTACAAAG
    CAGCCAATAA ATAGAGGTCA GTCTAAACCC GTGCTGCAGA AGCAGTCCAC TTTTCCCCAG
    TCTTCCAAAG ATATACCAGA CAGAGGGGCA GCAACAGATG AGAAATTACA GAATTTCGCT
    ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TAAGCTCTCT TAGTGACATT
    GATCAAGAAA ACAACAACAA CAAGGAAAAT GAACCTGTCA AAGAGACAGA GCCCCCTGCC
    TCACAGGGAG AACCAGGTAA ACCCCAGGCC TCAGGTTATG CTCCTAAATC GTTTCACGTG
    GAAGACACCC CTGTTTGTTT CTCAAGAAAC AGTTCTCTCA GTTCTCTTAG TATTGATTCT
    GAAGATGACC TGCTGCAGGA ATGTATAAGT TCTGCAATGC CAAAAAAGAA AAAGCCTTCA
    AGGCTCAAGC CCGATAATGA AAAGCATAGT CCCAGAAATA TGGGTGGCAT ATTAGCAGAA
    GATTTGACAC TCGATTTGAA AGATATACAG AGACCAGATT CAGAACATGG TTTATCCCCA
    GATTCAGAAA ATTTTGATTG GAAAGCTATT CAGGAAGGTG CAAATTCCAT AGTAAGTAGT
    TTACATCAAG CTGCTGCCGC TGCATGTTTA TCTCGACAAG CTTCATCTGA TTCAGATTCT
    ATCCTTTCAT TGAAATCGGG TATCTCTCTG GGATCACCAT TTCACCTTAC ACCTGATCAA
    GAAGAAAAAC CCTTTACGAG TAATAAAGGT CCACGAATTC TAAAACCTGG GGAGAAAAGT
    ACATTGGAAA CTAAAAAAAT AGAATCTGAA AATAAAGGAA TAAAAGGAGG CAAAAAGGTT
    TATAAAAGTT TGATTACTGG AAAAGTTCGA TCTAATTCGG AAATTTCGAG CCAAATGAAA
    CAACCCCTTC AAACAAACAT GCCTTCAATC TCTCGAGGTA GGACAATGAT TCATATTCCA
    GGAGTTCGGA ATAGCTCTTC AAGTACAAGC CCAGTTTCTA AAAAAGGCCC GCCCCTCAAG
    ACTCCAGCCT CCAAAAGCCC TAGTGAAGGT CAGCCGGCTA CCACCTCTCC CAGAGGAACC
    AAGCCATCAG TGAAGTCAGA ATTAAGCCCT GTTACAAGGC AGGCATCCCA GACAGCAGGA
    TCAAACAAAG GACCTTCTAG ATCAGGATCT AGAGATTCCA CTCCTTCAAG ACCTGCCCAG
    CAACCATTAA GTAGACCAAT GCAGTCTCCA GGGCGAAACT CAATCTCTCC TGGTAGAAAT
    GGAATAAGTC CCCCTAACAA ATTATCTCAA CTACCAAGGA CGTCATCCCC TAGTACTGCT
    TCAACTAAGT CCTCAGGTTC TGGGAAAATG TCTTACACAT CTCCTGGCAG ACAGATGAGC
    CAGCAGAACC TCACCAAACA AACAGGCTTA TCCAAGAATG GCAGTGGTAT CCCAAGAAGT
    GAATCTGCCT CCAAAGGGCT AAATCAAATG AGTAATAGTA ATGGATCCAA TAAAAAAGTA
    GAACTTTCTA GAATGTCTTC AACAAAGTCA AGTGGAAGTG AATCCGATAG GTCAGAGAGA
    CCTGTATTAG TACGCCAATC AACTTTCATC AAAGAAGCTC CAAGCCCAAC CCTAAGGAGA
    AAACTGGAGG AATCTGCTTC ATTTGAATCT CTTTCTCCAT CTTCCAGACC CGATTCTCCC
    ACACGGTCCC AGGCTCATAC TCCAGTTTTA AGTCCTTCCC TTCCTGATAT GTCTCTATCT
    ACACATTCGT CTCTTCAGTC TGGTGGATGG CGAAAACTCC CACCTAACCT CAGTCCCACC
    ATAGAGTATA ATGATGGAAG ACCAGTAAAG CGCCATGATA TAGCACGCTC TCATTCCGAA
    AGTCCTTCCA GACTTCCCAT CAATAGGTCA GGGACCTGGA AACGTGAGCA CAGCAAACAC
    TCATCATCAC TTCCTCGAGT AAGCACTTGG AGAAGAACTG GAAGTTCATC CTCAATTCTT
    TCTGCTTCAT CAGAATCTAG TGAAAAGGCA AAAAGTGAGG ACGAAAAACA AGTGAACTCT
    ATTTCAGGAA GCAAACAAAC TAAAGAAAAC CAGGTATCCA CAAAAGGAAC ATGGAGAAAA
    ATAAAAGAAA GTGAAATTTC TCCCACAAAT AGTACTTCTC AGACCACTTC TTCAGGTGCT
    GCAAATGGTG CTGAATCAAA GACTCTGATT TATCAAATGG CACCTGCTGT TTCTAAAACA
    GAGGATGTTT GGGTGAGAAT TGAGGATTGC CCCATTAACA ACCCTAGATC TGGAAGATCT
    CCAACAGGAA ATACTCCCCC CGTGATTGAC ACTGTTTCAG AAAAGGGAAA CCCGAACCCT
    AAAGATTCAA AAGATAATCA GGGAAAACAA AATGTGAGCA ATGGTAGTGC CCCTACACGC
    ACCATGGGTC TGGAAAACCG CCTGAATTCC TTTATTCAGG TAGATCCTCC AGACCAAAAA
    GGAACTGAGA CAAAACCGGG ACACAGTAAT AACCCTGTCC CTGCATCCGA GACTAGTGAA
    AGTTCTATAG CCGAGCGTAC CCCGTTTAGT TCTAGCAGCT CAAGCAAGCA CAGTTCACCA
    AGTGGGACTG TCGCCGCCAG GGTGAGTCCT TTTAATTACA ACCCAAGCCC AAGGAAGAGC
    AGCACAGATG GCACTTCAGC CCGACCGTCT CAGATCCCAA CGCCAGTGAG CAACAACACA
    AAGAAACGCG ACTCAAAACC CGACAGCACA GAACCCAGCG GGACTCAAAG TCCTAAACGC
    CATTCTGGGT CTTACCTTGT GACATCTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52405
    Clone ID Related Accession (Same CDS sequence) XM_010808816.2
    Accession Version XM_010808816.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCGCCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807118.1
    CDS142..8781
    Translation

    Target ORF information:

    RefSeq Version XM_010808816.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808816.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCGCCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91575
    Clone ID Related Accession (Same CDS sequence) XM_015472979.1
    Accession Version XM_015472979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGTA GGAAGGCTAC CCGGGGCATT
    TCATCCCAGG AGCTAGGGCA GGGGCTTTCA GGGGGACTAC AGGCCATTGC AGAATTATTG
    CAAGTGGACT GTGAAATGTA TGGGCTTACT AATGACCACT ACAGTATTAC TTTAAGACGA
    TATGCAGGAA TGGCTTTGAC AAACTTGACT TTCGGAGATG TAGCCAACAA GGCTACTCTC
    TGCTCTATGA AAGGCTGCAT GAGAGCGCTC GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC
    TTGCAGCAGG TTATTGCGAG TGTTTTGAGG AATCTGTCTT GGAGAGCAGA TGTAAATAGT
    AAAAAGACTT TGCGTGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTG
    AAAAAGGAAT CAACCCTCAA AAGTGTATTG AGTGCCTTAT GGAACTTGTC AGCACACTGC
    ACTGAGAATA AAGCTGACAT ATGTGCTGTG GATGGTGCGC TTGCATTTTT GGTTGGCACT
    CTCACTTATC GCAGCCAGAC CAATACTTTA GCCATTATTG AAAGTGGAGG TGGTATATTA
    CGGAATGTAT CCAGCTTGAT AGCTACAAAT GAGGACCACA GGCAAATCCT AAGAGAGAAT
    AATTGCTTAC AGACCTTATT ACAACACTTG AAATCTCACA GTTTGACAAT AGTCAGCAAT
    GCATGCGGAA CCTTGTGGAA TCTCTCAGCA AGAAACCCTA AAGACCAGGA AGCATTGTGG
    GACATGGGAG CAGTCAGCAT GCTCAAGAAC CTCATTCATT CAAAGCACAA GATGATTGCT
    ATGGGAAGTG CTGCAGCTTT AAGGAATCTC ATGGCAAATA GACCTGCAAA GTATAAAGAT
    GCCAATATCA TGTCTCCTGG TTCAAGTTTG CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC
    CTGGAAGCAG AATTAGATGC TCAGCATTTA TCAGAAACTT TTGACAATAT TGACAATTTA
    AGTCCCAAGG CATCTCATCG TAGCAAGCAG AGACACAAGC AAAATCTCTA TGGTGACTAT
    GTTTTTGACA CCAATCGACA TGATGATAAC AGGTCAGACA ATTTTAATAC TGGAAACATG
    ACTGTCCTAT CACCGTACTT AAACACTACA GTATTGCCCA GCTCTTCTTC ATCAAGGGGA
    AGTTTAGATA GCTCTCGTTC TGAGAAAGAT AGAAGTCTGG AGAGAGAACG AGGTATTAGC
    CTAGGCAACT ATCACCCAGC AACAGAAAAC CCAGGAACCT CTTCAAAGCG AGGTTTGCAG
    ATTTCTACCA CTGCAGCCCA GATTGCCAAA GTCATGGAAG AAGTATCAGC TATTCATACC
    TCTCAGGAAG ACAGAAGTTC TGGGTCTACC ACAGAACTAC ACTGTGGGAC AGATGAGAGG
    AATGCACTAA GAAGAAGCTC TACCACCCAC ACACATGCAA ACACGTACAA CTTTACCAAG
    TCAGAAAACT CAAACAGAAC ATGTCCAATA CCATATGCCA AAGTAGAATA TAAGAGATCT
    TCAAATGATA GTTTAAATAG TGTCAGCAGT AGTGATGGTT ATGGTAAAAG AGGTCAGATG
    AAACCTTCAA TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG CTATGGTCAG
    TATCCAGCTG ACCTAGCCCA TAAAATACAT AGTGCAAATC ATATGGATGA TAATGATGGA
    GAACTAGATA CACCAATAAA TTACAGTCTC AAATATTCCG ATGAACAGTT GAACTCTGGG
    AGGCAGAGCC CTTCACAGAA TGAAAGATGG GCAAGACCCA AACATATACT AGAAGATGAA
    ATAAAACCGA ATGAGCAGAG ACAATCAAGG AGTCAAAGCA CAGCTTATCC CGTGTATCCT
    GAGAGCACTG ATGATAAACA CCTCAAGTTC CAACCACACT TTGGGCAGCA AGAATGTGTT
    TCCCCATACA GGTCAAGAGC AGCCAATGGA TCTGAAACAA ATCGAGTAGG CTCTAATCAT
    GGAATTAGTC AAAATGTGAA CCAGTCTTTG TGTCAAGAAG ATGACTATGA AGACGATAAA
    CCAACCAACT ATAGCGAACG TTACTCTGAG GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA
    ACCAATTATA GCATAAAATA CAGTGAAGAA AAACATCACG TGGATCAGCC TATTGATTAT
    AGTTTAAAAT ACACCACAGA CATTCCTTCT TCACAGAAAC CAGCATTTTC ATTCTCAAAG
    AATTCATCTG GACAGAGCAC AAAAACTGAA CACATCTCTT CAAGCAGCGA GAATACATCC
    ACAACTTCAT CTAATGCCAA GAGGCAGAAT CAGCTGCATC CAAGCTCAGC ACAGAGCAGA
    AGTGGTCAGA CCCCAAAAGC CACCTCTTCC TCTTGCAAAG TCCCCTCTAT CAACCAAGAA
    ACAATACAGA CTTACTGTGT AGAAGATACC CCAATATGTT TTTCAAGATG CAGTTCATTA
    TCATCTCTGT CATCTGCTGA AGATGAAGTA GGGTGTGATC AGACAACACA AGAAGCAGAG
    TCTGCTAACA CTCTGCAAAT AGCTGAAATC AAGGACAACA GCGGACCTAG GTCAAATGAA
    GATTCTGTGA GTAAAGTTCC AGCAGGGTCA CAGCACATTA GAACCAAATC CAGCAGACTC
    CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC AGGCACAAAG CTGTTGAATT TTCTTCAGGG
    GCCAAATCTC CTTCCAAGAG CGGTGCTCAG ACACCCAAAA GTCCACCAGA GCACTACGTT
    CAGGAGACCC CACTCATGTT TAGCAGATGT ACTTCAGTCA GTTCACTCGA CAGTTTTGAG
    AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT GAACCCTGCA GTGGAATGGT AAGTGGCATC
    ATAAGCCCCA GTGACCTCCC AGATAGCCCT GGACAAACCA TGCCACCAAG CAGAAGCAAA
    ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT CAGACAGTTC AAACGAAGCA GGAAGTACCT
    AAAAATAAAG CACCTAGTGC TGAAAAGAGA GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT
    GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA GAGGCTGATA CTCTGTTACA TTTTGCCACA
    GAGAGTACTC CTGATGGATT TTCTTGTTCA TCTAGCCTGA GCGCTCTGAG CCTTGATGAG
    CCATTTATTC AGAAAGATGT GGAACTAAGA ATAATGCCTC CAGTTCAGGA AAATGACAAC
    GGGAATGAAA CAGAAAGTGA GCAGCCTGAA GAATCAAATG AAAACCAGGA AAAAGAGGCA
    GAAAAACCCA CTGATTCTGA AAAAGATCTT TTAGATGAGT CAGATGATGA TGATATTGAA
    ATACTAGAAG AGTGTATAAT TTCTGCCATG CCAACAAAAT CTTCACGCAA AGCCAAAAAA
    CCAGCCCAGA CTACTTCAAA ATTACCTCCA CCTGTGGCAA GGAAACCAAG TCAGCTCCCT
    GTATACAAAC TTCTGCCATC GCAAAACAGG TTACAGGCAC AAAAGCATGT TAGTTTTACA
    CCAGGAGATG ATATGCCACG GGTGTATTGT GTAGAAGGGA CACCTATAAA CTTTTCCACA
    GCTACATCTC TGAGTGATCT AACGATAGAA TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA
    GGGGTTAGAG CAGGGGCACA GTCAAGTGAG TTTGAAAAAC GAGATACCAT TCCTACTGAA
    GGCAGAAGTA CAGATGAGGC TCAACGAGGG AAAGCCTCAT CTGTCACTGT GCCTGAACTG
    GACGACAGCA AAACAGAAGA AGGTGATATT CTTGCAGAAT GCATTAATTC TGCCATGCCC
    AAAGGAAAAA GTCACAAGCC TTTCCGTGTG AAAAAGATCA TGGACCAGGT CCAGCAAGCA
    TCTATGTCTT CATCTGGAAC TAACAAAAAT CAATTAGATG GTAAGACGAA GAAACCTACT
    TCACCAGTAA AACCTATACC ACAAAATACT GAATACAGGA CACGTGTAAG AAAAAATACA
    GACTCAAAAA ATAATCTAAA TGCAGAAAGA AATTTCTCGG AAAACAAAGA CTCAAAGAAA
    CAGCACTTGA AAAATAATTC CAAGGACTTC AATGATAAAC TGCCAAATAA TGAAGACAGA
    GTCAGAGGAA GTTTTACTTT TGATTCACCC CATCATTACA CGCCCATTGA AGGCACTCCG
    TACTGTTTTT CACGAAATGA CTCTCTGAGT TCTCTAGATT TTGATGATGA TGATGTTGAC
    CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG GGGAAGGAAA ATAAAGAATC AGAAGCTAAA
    GTGACCAACC ACACAGAACT AACCTCAAAC CAACAATCAG CTAGTAAGAC ACCAGCTGTT
    ACAAAGCAGC CAATAAATAG AGGTCAGTCT AAACCCGTGC TGCAGAAGCA GTCCACTTTT
    CCCCAGTCTT CCAAAGATAT ACCAGACAGA GGGGCAGCAA CAGATGAGAA ATTACAGAAT
    TTCGCTATTG AAAATACTCC AGTTTGCTTT TCTCGAAATT CCTCTCTAAG CTCTCTTAGT
    GACATTGATC AAGAAAACAA CAACAACAAG GAAAATGAAC CTGTCAAAGA GACAGAGCCC
    CCTGCCTCAC AGGGAGAACC AGGTAAACCC CAGGCCTCAG GTTATGCTCC TAAATCGTTT
    CACGTGGAAG ACACCCCTGT TTGTTTCTCA AGAAACAGTT CTCTCAGTTC TCTTAGTATT
    GATTCTGAAG ATGACCTGCT GCAGGAATGT ATAAGTTCTG CAATGCCAAA AAAGAAAAAG
    CCTTCAAGGC TCAAGCCCGA TAATGAAAAG CATAGTCCCA GAAATATGGG TGGCATATTA
    GCAGAAGATT TGACACTCGA TTTGAAAGAT ATACAGAGAC CAGATTCAGA ACATGGTTTA
    TCCCCAGATT CAGAAAATTT TGATTGGAAA GCTATTCAGG AAGGTGCAAA TTCCATAGTA
    AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA TGTTTATCTC GACAAGCTTC ATCTGATTCA
    GATTCTATCC TTTCATTGAA ATCGGGTATC TCTCTGGGAT CACCATTTCA CCTTACACCT
    GATCAAGAAG AAAAACCCTT TACGAGTAAT AAAGGTCCAC GAATTCTAAA ACCTGGGGAG
    AAAAGTACAT TGGAAACTAA AAAAATAGAA TCTGAAAATA AAGGAATAAA AGGAGGCAAA
    AAGGTTTATA AAAGTTTGAT TACTGGAAAA GTTCGATCTA ATTCGGAAAT TTCGAGCCAA
    ATGAAACAAC CCCTTCAAAC AAACATGCCT TCAATCTCTC GAGGTAGGAC AATGATTCAT
    ATTCCAGGAG TTCGGAATAG CTCTTCAAGT ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC
    CTCAAGACTC CAGCCTCCAA AAGCCCTAGT GAAGGTCAGC CGGCTACCAC CTCTCCCAGA
    GGAACCAAGC CATCAGTGAA GTCAGAATTA AGCCCTGTTA CAAGGCAGGC ATCCCAGACA
    GCAGGATCAA ACAAAGGACC TTCTAGATCA GGATCTAGAG ATTCCACTCC TTCAAGACCT
    GCCCAGCAAC CATTAAGTAG ACCAATGCAG TCTCCAGGGC GAAACTCAAT CTCTCCTGGT
    AGAAATGGAA TAAGTCCCCC TAACAAATTA TCTCAACTAC CAAGGACGTC ATCCCCTAGT
    ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG AAAATGTCTT ACACATCTCC TGGCAGACAG
    ATGAGCCAGC AGAACCTCAC CAAACAAACA GGCTTATCCA AGAATGGCAG TGGTATCCCA
    AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT CAAATGAGTA ATAGTAATGG ATCCAATAAA
    AAAGTAGAAC TTTCTAGAAT GTCTTCAACA AAGTCAAGTG GAAGTGAATC CGATAGGTCA
    GAGAGACCTG TATTAGTACG CCAATCAACT TTCATCAAAG AAGCTCCAAG CCCAACCCTA
    AGGAGAAAAC TGGAGGAATC TGCTTCATTT GAATCTCTTT CTCCATCTTC CAGACCCGAT
    TCTCCCACAC GGTCCCAGGC TCATACTCCA GTTTTAAGTC CTTCCCTTCC TGATATGTCT
    CTATCTACAC ATTCGTCTCT TCAGTCTGGT GGATGGCGAA AACTCCCACC TAACCTCAGT
    CCCACCATAG AGTATAATGA TGGAAGACCA GTAAAGCGCC ATGATATAGC ACGCTCTCAT
    TCCGAAAGTC CTTCCAGACT TCCCATCAAT AGGTCAGGGA CCTGGAAACG TGAGCACAGC
    AAACACTCAT CATCACTTCC TCGAGTAAGC ACTTGGAGAA GAACTGGAAG TTCATCCTCA
    ATTCTTTCTG CTTCATCAGA ATCTAGTGAA AAGGCAAAAA GTGAGGACGA AAAACAAGTG
    AACTCTATTT CAGGAAGCAA ACAAACTAAA GAAAACCAGG TATCCACAAA AGGAACATGG
    AGAAAAATAA AAGAAAGTGA AATTTCTCCC ACAAATAGTA CTTCTCAGAC CACTTCTTCA
    GGTGCTGCAA ATGGTGCTGA ATCAAAGACT CTGATTTATC AAATGGCACC TGCTGTTTCT
    AAAACAGAGG ATGTTTGGGT GAGAATTGAG GATTGCCCCA TTAACAACCC TAGATCTGGA
    AGATCTCCAA CAGGAAATAC TCCCCCCGTG ATTGACACTG TTTCAGAAAA GGGAAACCCG
    AACCCTAAAG ATTCAAAAGA TAATCAGGGA AAACAAAATG TGAGCAATGG TAGTGCCCCT
    ACACGCACCA TGGGTCTGGA AAACCGCCTG AATTCCTTTA TTCAGGTAGA TCCTCCAGAC
    CAAAAAGGAA CTGAGACAAA ACCGGGACAC AGTAATAACC CTGTCCCTGC ATCCGAGACT
    AGTGAAAGTT CTATAGCCGA GCGTACCCCG TTTAGTTCTA GCAGCTCAAG CAAGCACAGT
    TCACCAAGTG GGACTGTCGC CGCCAGGGTG AGTCCTTTTA ATTACAACCC AAGCCCAAGG
    AAGAGCAGCA CAGATGGCAC TTCAGCCCGA CCGTCTCAGA TCCCAACGCC AGTGAGCAAC
    AACACAAAGA AACGCGACTC AAAACCCGAC AGCACAGAAC CCAGCGGGAC TCAAAGTCCT
    AAACGCCATT CTGGGTCTTA CCTTGTGACA TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328465.1
    CDS143..8881
    Translation

    Target ORF information:

    RefSeq Version XM_015472979.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGTA GGAAGGCTAC CCGGGGCATT
    TCATCCCAGG AGCTAGGGCA GGGGCTTTCA GGGGGACTAC AGGCCATTGC AGAATTATTG
    CAAGTGGACT GTGAAATGTA TGGGCTTACT AATGACCACT ACAGTATTAC TTTAAGACGA
    TATGCAGGAA TGGCTTTGAC AAACTTGACT TTCGGAGATG TAGCCAACAA GGCTACTCTC
    TGCTCTATGA AAGGCTGCAT GAGAGCGCTC GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC
    TTGCAGCAGG TTATTGCGAG TGTTTTGAGG AATCTGTCTT GGAGAGCAGA TGTAAATAGT
    AAAAAGACTT TGCGTGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTG
    AAAAAGGAAT CAACCCTCAA AAGTGTATTG AGTGCCTTAT GGAACTTGTC AGCACACTGC
    ACTGAGAATA AAGCTGACAT ATGTGCTGTG GATGGTGCGC TTGCATTTTT GGTTGGCACT
    CTCACTTATC GCAGCCAGAC CAATACTTTA GCCATTATTG AAAGTGGAGG TGGTATATTA
    CGGAATGTAT CCAGCTTGAT AGCTACAAAT GAGGACCACA GGCAAATCCT AAGAGAGAAT
    AATTGCTTAC AGACCTTATT ACAACACTTG AAATCTCACA GTTTGACAAT AGTCAGCAAT
    GCATGCGGAA CCTTGTGGAA TCTCTCAGCA AGAAACCCTA AAGACCAGGA AGCATTGTGG
    GACATGGGAG CAGTCAGCAT GCTCAAGAAC CTCATTCATT CAAAGCACAA GATGATTGCT
    ATGGGAAGTG CTGCAGCTTT AAGGAATCTC ATGGCAAATA GACCTGCAAA GTATAAAGAT
    GCCAATATCA TGTCTCCTGG TTCAAGTTTG CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC
    CTGGAAGCAG AATTAGATGC TCAGCATTTA TCAGAAACTT TTGACAATAT TGACAATTTA
    AGTCCCAAGG CATCTCATCG TAGCAAGCAG AGACACAAGC AAAATCTCTA TGGTGACTAT
    GTTTTTGACA CCAATCGACA TGATGATAAC AGGTCAGACA ATTTTAATAC TGGAAACATG
    ACTGTCCTAT CACCGTACTT AAACACTACA GTATTGCCCA GCTCTTCTTC ATCAAGGGGA
    AGTTTAGATA GCTCTCGTTC TGAGAAAGAT AGAAGTCTGG AGAGAGAACG AGGTATTAGC
    CTAGGCAACT ATCACCCAGC AACAGAAAAC CCAGGAACCT CTTCAAAGCG AGGTTTGCAG
    ATTTCTACCA CTGCAGCCCA GATTGCCAAA GTCATGGAAG AAGTATCAGC TATTCATACC
    TCTCAGGAAG ACAGAAGTTC TGGGTCTACC ACAGAACTAC ACTGTGGGAC AGATGAGAGG
    AATGCACTAA GAAGAAGCTC TACCACCCAC ACACATGCAA ACACGTACAA CTTTACCAAG
    TCAGAAAACT CAAACAGAAC ATGTCCAATA CCATATGCCA AAGTAGAATA TAAGAGATCT
    TCAAATGATA GTTTAAATAG TGTCAGCAGT AGTGATGGTT ATGGTAAAAG AGGTCAGATG
    AAACCTTCAA TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG CTATGGTCAG
    TATCCAGCTG ACCTAGCCCA TAAAATACAT AGTGCAAATC ATATGGATGA TAATGATGGA
    GAACTAGATA CACCAATAAA TTACAGTCTC AAATATTCCG ATGAACAGTT GAACTCTGGG
    AGGCAGAGCC CTTCACAGAA TGAAAGATGG GCAAGACCCA AACATATACT AGAAGATGAA
    ATAAAACCGA ATGAGCAGAG ACAATCAAGG AGTCAAAGCA CAGCTTATCC CGTGTATCCT
    GAGAGCACTG ATGATAAACA CCTCAAGTTC CAACCACACT TTGGGCAGCA AGAATGTGTT
    TCCCCATACA GGTCAAGAGC AGCCAATGGA TCTGAAACAA ATCGAGTAGG CTCTAATCAT
    GGAATTAGTC AAAATGTGAA CCAGTCTTTG TGTCAAGAAG ATGACTATGA AGACGATAAA
    CCAACCAACT ATAGCGAACG TTACTCTGAG GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA
    ACCAATTATA GCATAAAATA CAGTGAAGAA AAACATCACG TGGATCAGCC TATTGATTAT
    AGTTTAAAAT ACACCACAGA CATTCCTTCT TCACAGAAAC CAGCATTTTC ATTCTCAAAG
    AATTCATCTG GACAGAGCAC AAAAACTGAA CACATCTCTT CAAGCAGCGA GAATACATCC
    ACAACTTCAT CTAATGCCAA GAGGCAGAAT CAGCTGCATC CAAGCTCAGC ACAGAGCAGA
    AGTGGTCAGA CCCCAAAAGC CACCTCTTCC TCTTGCAAAG TCCCCTCTAT CAACCAAGAA
    ACAATACAGA CTTACTGTGT AGAAGATACC CCAATATGTT TTTCAAGATG CAGTTCATTA
    TCATCTCTGT CATCTGCTGA AGATGAAGTA GGGTGTGATC AGACAACACA AGAAGCAGAG
    TCTGCTAACA CTCTGCAAAT AGCTGAAATC AAGGACAACA GCGGACCTAG GTCAAATGAA
    GATTCTGTGA GTAAAGTTCC AGCAGGGTCA CAGCACATTA GAACCAAATC CAGCAGACTC
    CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC AGGCACAAAG CTGTTGAATT TTCTTCAGGG
    GCCAAATCTC CTTCCAAGAG CGGTGCTCAG ACACCCAAAA GTCCACCAGA GCACTACGTT
    CAGGAGACCC CACTCATGTT TAGCAGATGT ACTTCAGTCA GTTCACTCGA CAGTTTTGAG
    AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT GAACCCTGCA GTGGAATGGT AAGTGGCATC
    ATAAGCCCCA GTGACCTCCC AGATAGCCCT GGACAAACCA TGCCACCAAG CAGAAGCAAA
    ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT CAGACAGTTC AAACGAAGCA GGAAGTACCT
    AAAAATAAAG CACCTAGTGC TGAAAAGAGA GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT
    GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA GAGGCTGATA CTCTGTTACA TTTTGCCACA
    GAGAGTACTC CTGATGGATT TTCTTGTTCA TCTAGCCTGA GCGCTCTGAG CCTTGATGAG
    CCATTTATTC AGAAAGATGT GGAACTAAGA ATAATGCCTC CAGTTCAGGA AAATGACAAC
    GGGAATGAAA CAGAAAGTGA GCAGCCTGAA GAATCAAATG AAAACCAGGA AAAAGAGGCA
    GAAAAACCCA CTGATTCTGA AAAAGATCTT TTAGATGAGT CAGATGATGA TGATATTGAA
    ATACTAGAAG AGTGTATAAT TTCTGCCATG CCAACAAAAT CTTCACGCAA AGCCAAAAAA
    CCAGCCCAGA CTACTTCAAA ATTACCTCCA CCTGTGGCAA GGAAACCAAG TCAGCTCCCT
    GTATACAAAC TTCTGCCATC GCAAAACAGG TTACAGGCAC AAAAGCATGT TAGTTTTACA
    CCAGGAGATG ATATGCCACG GGTGTATTGT GTAGAAGGGA CACCTATAAA CTTTTCCACA
    GCTACATCTC TGAGTGATCT AACGATAGAA TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA
    GGGGTTAGAG CAGGGGCACA GTCAAGTGAG TTTGAAAAAC GAGATACCAT TCCTACTGAA
    GGCAGAAGTA CAGATGAGGC TCAACGAGGG AAAGCCTCAT CTGTCACTGT GCCTGAACTG
    GACGACAGCA AAACAGAAGA AGGTGATATT CTTGCAGAAT GCATTAATTC TGCCATGCCC
    AAAGGAAAAA GTCACAAGCC TTTCCGTGTG AAAAAGATCA TGGACCAGGT CCAGCAAGCA
    TCTATGTCTT CATCTGGAAC TAACAAAAAT CAATTAGATG GTAAGACGAA GAAACCTACT
    TCACCAGTAA AACCTATACC ACAAAATACT GAATACAGGA CACGTGTAAG AAAAAATACA
    GACTCAAAAA ATAATCTAAA TGCAGAAAGA AATTTCTCGG AAAACAAAGA CTCAAAGAAA
    CAGCACTTGA AAAATAATTC CAAGGACTTC AATGATAAAC TGCCAAATAA TGAAGACAGA
    GTCAGAGGAA GTTTTACTTT TGATTCACCC CATCATTACA CGCCCATTGA AGGCACTCCG
    TACTGTTTTT CACGAAATGA CTCTCTGAGT TCTCTAGATT TTGATGATGA TGATGTTGAC
    CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG GGGAAGGAAA ATAAAGAATC AGAAGCTAAA
    GTGACCAACC ACACAGAACT AACCTCAAAC CAACAATCAG CTAGTAAGAC ACCAGCTGTT
    ACAAAGCAGC CAATAAATAG AGGTCAGTCT AAACCCGTGC TGCAGAAGCA GTCCACTTTT
    CCCCAGTCTT CCAAAGATAT ACCAGACAGA GGGGCAGCAA CAGATGAGAA ATTACAGAAT
    TTCGCTATTG AAAATACTCC AGTTTGCTTT TCTCGAAATT CCTCTCTAAG CTCTCTTAGT
    GACATTGATC AAGAAAACAA CAACAACAAG GAAAATGAAC CTGTCAAAGA GACAGAGCCC
    CCTGCCTCAC AGGGAGAACC AGGTAAACCC CAGGCCTCAG GTTATGCTCC TAAATCGTTT
    CACGTGGAAG ACACCCCTGT TTGTTTCTCA AGAAACAGTT CTCTCAGTTC TCTTAGTATT
    GATTCTGAAG ATGACCTGCT GCAGGAATGT ATAAGTTCTG CAATGCCAAA AAAGAAAAAG
    CCTTCAAGGC TCAAGCCCGA TAATGAAAAG CATAGTCCCA GAAATATGGG TGGCATATTA
    GCAGAAGATT TGACACTCGA TTTGAAAGAT ATACAGAGAC CAGATTCAGA ACATGGTTTA
    TCCCCAGATT CAGAAAATTT TGATTGGAAA GCTATTCAGG AAGGTGCAAA TTCCATAGTA
    AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA TGTTTATCTC GACAAGCTTC ATCTGATTCA
    GATTCTATCC TTTCATTGAA ATCGGGTATC TCTCTGGGAT CACCATTTCA CCTTACACCT
    GATCAAGAAG AAAAACCCTT TACGAGTAAT AAAGGTCCAC GAATTCTAAA ACCTGGGGAG
    AAAAGTACAT TGGAAACTAA AAAAATAGAA TCTGAAAATA AAGGAATAAA AGGAGGCAAA
    AAGGTTTATA AAAGTTTGAT TACTGGAAAA GTTCGATCTA ATTCGGAAAT TTCGAGCCAA
    ATGAAACAAC CCCTTCAAAC AAACATGCCT TCAATCTCTC GAGGTAGGAC AATGATTCAT
    ATTCCAGGAG TTCGGAATAG CTCTTCAAGT ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC
    CTCAAGACTC CAGCCTCCAA AAGCCCTAGT GAAGGTCAGC CGGCTACCAC CTCTCCCAGA
    GGAACCAAGC CATCAGTGAA GTCAGAATTA AGCCCTGTTA CAAGGCAGGC ATCCCAGACA
    GCAGGATCAA ACAAAGGACC TTCTAGATCA GGATCTAGAG ATTCCACTCC TTCAAGACCT
    GCCCAGCAAC CATTAAGTAG ACCAATGCAG TCTCCAGGGC GAAACTCAAT CTCTCCTGGT
    AGAAATGGAA TAAGTCCCCC TAACAAATTA TCTCAACTAC CAAGGACGTC ATCCCCTAGT
    ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG AAAATGTCTT ACACATCTCC TGGCAGACAG
    ATGAGCCAGC AGAACCTCAC CAAACAAACA GGCTTATCCA AGAATGGCAG TGGTATCCCA
    AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT CAAATGAGTA ATAGTAATGG ATCCAATAAA
    AAAGTAGAAC TTTCTAGAAT GTCTTCAACA AAGTCAAGTG GAAGTGAATC CGATAGGTCA
    GAGAGACCTG TATTAGTACG CCAATCAACT TTCATCAAAG AAGCTCCAAG CCCAACCCTA
    AGGAGAAAAC TGGAGGAATC TGCTTCATTT GAATCTCTTT CTCCATCTTC CAGACCCGAT
    TCTCCCACAC GGTCCCAGGC TCATACTCCA GTTTTAAGTC CTTCCCTTCC TGATATGTCT
    CTATCTACAC ATTCGTCTCT TCAGTCTGGT GGATGGCGAA AACTCCCACC TAACCTCAGT
    CCCACCATAG AGTATAATGA TGGAAGACCA GTAAAGCGCC ATGATATAGC ACGCTCTCAT
    TCCGAAAGTC CTTCCAGACT TCCCATCAAT AGGTCAGGGA CCTGGAAACG TGAGCACAGC
    AAACACTCAT CATCACTTCC TCGAGTAAGC ACTTGGAGAA GAACTGGAAG TTCATCCTCA
    ATTCTTTCTG CTTCATCAGA ATCTAGTGAA AAGGCAAAAA GTGAGGACGA AAAACAAGTG
    AACTCTATTT CAGGAAGCAA ACAAACTAAA GAAAACCAGG TATCCACAAA AGGAACATGG
    AGAAAAATAA AAGAAAGTGA AATTTCTCCC ACAAATAGTA CTTCTCAGAC CACTTCTTCA
    GGTGCTGCAA ATGGTGCTGA ATCAAAGACT CTGATTTATC AAATGGCACC TGCTGTTTCT
    AAAACAGAGG ATGTTTGGGT GAGAATTGAG GATTGCCCCA TTAACAACCC TAGATCTGGA
    AGATCTCCAA CAGGAAATAC TCCCCCCGTG ATTGACACTG TTTCAGAAAA GGGAAACCCG
    AACCCTAAAG ATTCAAAAGA TAATCAGGGA AAACAAAATG TGAGCAATGG TAGTGCCCCT
    ACACGCACCA TGGGTCTGGA AAACCGCCTG AATTCCTTTA TTCAGGTAGA TCCTCCAGAC
    CAAAAAGGAA CTGAGACAAA ACCGGGACAC AGTAATAACC CTGTCCCTGC ATCCGAGACT
    AGTGAAAGTT CTATAGCCGA GCGTACCCCG TTTAGTTCTA GCAGCTCAAG CAAGCACAGT
    TCACCAAGTG GGACTGTCGC CGCCAGGGTG AGTCCTTTTA ATTACAACCC AAGCCCAAGG
    AAGAGCAGCA CAGATGGCAC TTCAGCCCGA CCGTCTCAGA TCCCAACGCC AGTGAGCAAC
    AACACAAAGA AACGCGACTC AAAACCCGAC AGCACAGAAC CCAGCGGGAC TCAAAGTCCT
    AAACGCCATT CTGGGTCTTA CCTTGTGACA TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91576
    Clone ID Related Accession (Same CDS sequence) XM_015472980.1 , XM_015472981.1 , XM_015472982.1
    Accession Version XM_015472980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328466.1
    CDS121..8829
    Translation

    Target ORF information:

    RefSeq Version XM_015472980.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91576
    Clone ID Related Accession (Same CDS sequence) XM_015472980.1 , XM_015472981.1 , XM_015472982.1
    Accession Version XM_015472981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328467.1
    CDS89..8797
    Translation

    Target ORF information:

    RefSeq Version XM_015472981.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91576
    Clone ID Related Accession (Same CDS sequence) XM_015472980.1 , XM_015472981.1 , XM_015472982.1
    Accession Version XM_015472982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1141
    1201
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    1321
    1381
    1441
    1501
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    1621
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    1801
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    1981
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    7921
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    8401
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    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328468.1
    CDS38..8746
    Translation

    Target ORF information:

    RefSeq Version XM_015472982.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCCCTGAA GATGGAGAAC 
    TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG
    GCATCTAATA TGAAGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91577
    Clone ID Related Accession (Same CDS sequence) XM_015472983.1
    Accession Version XM_015472983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGGG GACTACAGGC CATTGCAGAA
    TTATTGCAAG TGGACTGTGA AATGTATGGG CTTACTAATG ACCACTACAG TATTACTTTA
    AGACGATATG CAGGAATGGC TTTGACAAAC TTGACTTTCG GAGATGTAGC CAACAAGGCT
    ACTCTCTGCT CTATGAAAGG CTGCATGAGA GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC
    GAGGACTTGC AGCAGGTTAT TGCGAGTGTT TTGAGGAATC TGTCTTGGAG AGCAGATGTA
    AATAGTAAAA AGACTTTGCG TGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG
    GAAGTGAAAA AGGAATCAAC CCTCAAAAGT GTATTGAGTG CCTTATGGAA CTTGTCAGCA
    CACTGCACTG AGAATAAAGC TGACATATGT GCTGTGGATG GTGCGCTTGC ATTTTTGGTT
    GGCACTCTCA CTTATCGCAG CCAGACCAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGT
    ATATTACGGA ATGTATCCAG CTTGATAGCT ACAAATGAGG ACCACAGGCA AATCCTAAGA
    GAGAATAATT GCTTACAGAC CTTATTACAA CACTTGAAAT CTCACAGTTT GACAATAGTC
    AGCAATGCAT GCGGAACCTT GTGGAATCTC TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA
    TTGTGGGACA TGGGAGCAGT CAGCATGCTC AAGAACCTCA TTCATTCAAA GCACAAGATG
    ATTGCTATGG GAAGTGCTGC AGCTTTAAGG AATCTCATGG CAAATAGACC TGCAAAGTAT
    AAAGATGCCA ATATCATGTC TCCTGGTTCA AGTTTGCCTT CTCTACATGT CAGGAAACAA
    AAGGCCCTGG AAGCAGAATT AGATGCTCAG CATTTATCAG AAACTTTTGA CAATATTGAC
    AATTTAAGTC CCAAGGCATC TCATCGTAGC AAGCAGAGAC ACAAGCAAAA TCTCTATGGT
    GACTATGTTT TTGACACCAA TCGACATGAT GATAACAGGT CAGACAATTT TAATACTGGA
    AACATGACTG TCCTATCACC GTACTTAAAC ACTACAGTAT TGCCCAGCTC TTCTTCATCA
    AGGGGAAGTT TAGATAGCTC TCGTTCTGAG AAAGATAGAA GTCTGGAGAG AGAACGAGGT
    ATTAGCCTAG GCAACTATCA CCCAGCAACA GAAAACCCAG GAACCTCTTC AAAGCGAGGT
    TTGCAGATTT CTACCACTGC AGCCCAGATT GCCAAAGTCA TGGAAGAAGT ATCAGCTATT
    CATACCTCTC AGGAAGACAG AAGTTCTGGG TCTACCACAG AACTACACTG TGGGACAGAT
    GAGAGGAATG CACTAAGAAG AAGCTCTACC ACCCACACAC ATGCAAACAC GTACAACTTT
    ACCAAGTCAG AAAACTCAAA CAGAACATGT CCAATACCAT ATGCCAAAGT AGAATATAAG
    AGATCTTCAA ATGATAGTTT AAATAGTGTC AGCAGTAGTG ATGGTTATGG TAAAAGAGGT
    CAGATGAAAC CTTCAATTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGCTAT
    GGTCAGTATC CAGCTGACCT AGCCCATAAA ATACATAGTG CAAATCATAT GGATGATAAT
    GATGGAGAAC TAGATACACC AATAAATTAC AGTCTCAAAT ATTCCGATGA ACAGTTGAAC
    TCTGGGAGGC AGAGCCCTTC ACAGAATGAA AGATGGGCAA GACCCAAACA TATACTAGAA
    GATGAAATAA AACCGAATGA GCAGAGACAA TCAAGGAGTC AAAGCACAGC TTATCCCGTG
    TATCCTGAGA GCACTGATGA TAAACACCTC AAGTTCCAAC CACACTTTGG GCAGCAAGAA
    TGTGTTTCCC CATACAGGTC AAGAGCAGCC AATGGATCTG AAACAAATCG AGTAGGCTCT
    AATCATGGAA TTAGTCAAAA TGTGAACCAG TCTTTGTGTC AAGAAGATGA CTATGAAGAC
    GATAAACCAA CCAACTATAG CGAACGTTAC TCTGAGGAAG GGCAGCATGA GGAAGAAGAG
    AGGCCAACCA ATTATAGCAT AAAATACAGT GAAGAAAAAC ATCACGTGGA TCAGCCTATT
    GATTATAGTT TAAAATACAC CACAGACATT CCTTCTTCAC AGAAACCAGC ATTTTCATTC
    TCAAAGAATT CATCTGGACA GAGCACAAAA ACTGAACACA TCTCTTCAAG CAGCGAGAAT
    ACATCCACAA CTTCATCTAA TGCCAAGAGG CAGAATCAGC TGCATCCAAG CTCAGCACAG
    AGCAGAAGTG GTCAGACCCC AAAAGCCACC TCTTCCTCTT GCAAAGTCCC CTCTATCAAC
    CAAGAAACAA TACAGACTTA CTGTGTAGAA GATACCCCAA TATGTTTTTC AAGATGCAGT
    TCATTATCAT CTCTGTCATC TGCTGAAGAT GAAGTAGGGT GTGATCAGAC AACACAAGAA
    GCAGAGTCTG CTAACACTCT GCAAATAGCT GAAATCAAGG ACAACAGCGG ACCTAGGTCA
    AATGAAGATT CTGTGAGTAA AGTTCCAGCA GGGTCACAGC ACATTAGAAC CAAATCCAGC
    AGACTCCAGG CTTCTGGTCT GTCTTCAGAG TCAGCCAGGC ACAAAGCTGT TGAATTTTCT
    TCAGGGGCCA AATCTCCTTC CAAGAGCGGT GCTCAGACAC CCAAAAGTCC ACCAGAGCAC
    TACGTTCAGG AGACCCCACT CATGTTTAGC AGATGTACTT CAGTCAGTTC ACTCGACAGT
    TTTGAGAGTC GCTCGATTGC CAGCTCTGTT CAGAGTGAAC CCTGCAGTGG AATGGTAAGT
    GGCATCATAA GCCCCAGTGA CCTCCCAGAT AGCCCTGGAC AAACCATGCC ACCAAGCAGA
    AGCAAAACCC CTCCGCCGCC GCCTCCGCCT CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA
    GTACCTAAAA ATAAAGCACC TAGTGCTGAA AAGAGAGAAA GTGGACCCAA GCAAGCTGCT
    GTAAATGCTG CAGTACAAAG GGTCCAGGTT CTTCCAGAGG CTGATACTCT GTTACATTTT
    GCCACAGAGA GTACTCCTGA TGGATTTTCT TGTTCATCTA GCCTGAGCGC TCTGAGCCTT
    GATGAGCCAT TTATTCAGAA AGATGTGGAA CTAAGAATAA TGCCTCCAGT TCAGGAAAAT
    GACAACGGGA ATGAAACAGA AAGTGAGCAG CCTGAAGAAT CAAATGAAAA CCAGGAAAAA
    GAGGCAGAAA AACCCACTGA TTCTGAAAAA GATCTTTTAG ATGAGTCAGA TGATGATGAT
    ATTGAAATAC TAGAAGAGTG TATAATTTCT GCCATGCCAA CAAAATCTTC ACGCAAAGCC
    AAAAAACCAG CCCAGACTAC TTCAAAATTA CCTCCACCTG TGGCAAGGAA ACCAAGTCAG
    CTCCCTGTAT ACAAACTTCT GCCATCGCAA AACAGGTTAC AGGCACAAAA GCATGTTAGT
    TTTACACCAG GAGATGATAT GCCACGGGTG TATTGTGTAG AAGGGACACC TATAAACTTT
    TCCACAGCTA CATCTCTGAG TGATCTAACG ATAGAATCCC CTCCAAATGA GTTAGCTGCT
    GGAGAAGGGG TTAGAGCAGG GGCACAGTCA AGTGAGTTTG AAAAACGAGA TACCATTCCT
    ACTGAAGGCA GAAGTACAGA TGAGGCTCAA CGAGGGAAAG CCTCATCTGT CACTGTGCCT
    GAACTGGACG ACAGCAAAAC AGAAGAAGGT GATATTCTTG CAGAATGCAT TAATTCTGCC
    ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC CGTGTGAAAA AGATCATGGA CCAGGTCCAG
    CAAGCATCTA TGTCTTCATC TGGAACTAAC AAAAATCAAT TAGATGGTAA GACGAAGAAA
    CCTACTTCAC CAGTAAAACC TATACCACAA AATACTGAAT ACAGGACACG TGTAAGAAAA
    AATACAGACT CAAAAAATAA TCTAAATGCA GAAAGAAATT TCTCGGAAAA CAAAGACTCA
    AAGAAACAGC ACTTGAAAAA TAATTCCAAG GACTTCAATG ATAAACTGCC AAATAATGAA
    GACAGAGTCA GAGGAAGTTT TACTTTTGAT TCACCCCATC ATTACACGCC CATTGAAGGC
    ACTCCGTACT GTTTTTCACG AAATGACTCT CTGAGTTCTC TAGATTTTGA TGATGATGAT
    GTTGACCTTT CCAGGGAAAA GGCTGAATTA AGAAAGGGGA AGGAAAATAA AGAATCAGAA
    GCTAAAGTGA CCAACCACAC AGAACTAACC TCAAACCAAC AATCAGCTAG TAAGACACCA
    GCTGTTACAA AGCAGCCAAT AAATAGAGGT CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC
    ACTTTTCCCC AGTCTTCCAA AGATATACCA GACAGAGGGG CAGCAACAGA TGAGAAATTA
    CAGAATTTCG CTATTGAAAA TACTCCAGTT TGCTTTTCTC GAAATTCCTC TCTAAGCTCT
    CTTAGTGACA TTGATCAAGA AAACAACAAC AACAAGGAAA ATGAACCTGT CAAAGAGACA
    GAGCCCCCTG CCTCACAGGG AGAACCAGGT AAACCCCAGG CCTCAGGTTA TGCTCCTAAA
    TCGTTTCACG TGGAAGACAC CCCTGTTTGT TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT
    AGTATTGATT CTGAAGATGA CCTGCTGCAG GAATGTATAA GTTCTGCAAT GCCAAAAAAG
    AAAAAGCCTT CAAGGCTCAA GCCCGATAAT GAAAAGCATA GTCCCAGAAA TATGGGTGGC
    ATATTAGCAG AAGATTTGAC ACTCGATTTG AAAGATATAC AGAGACCAGA TTCAGAACAT
    GGTTTATCCC CAGATTCAGA AAATTTTGAT TGGAAAGCTA TTCAGGAAGG TGCAAATTCC
    ATAGTAAGTA GTTTACATCA AGCTGCTGCC GCTGCATGTT TATCTCGACA AGCTTCATCT
    GATTCAGATT CTATCCTTTC ATTGAAATCG GGTATCTCTC TGGGATCACC ATTTCACCTT
    ACACCTGATC AAGAAGAAAA ACCCTTTACG AGTAATAAAG GTCCACGAAT TCTAAAACCT
    GGGGAGAAAA GTACATTGGA AACTAAAAAA ATAGAATCTG AAAATAAAGG AATAAAAGGA
    GGCAAAAAGG TTTATAAAAG TTTGATTACT GGAAAAGTTC GATCTAATTC GGAAATTTCG
    AGCCAAATGA AACAACCCCT TCAAACAAAC ATGCCTTCAA TCTCTCGAGG TAGGACAATG
    ATTCATATTC CAGGAGTTCG GAATAGCTCT TCAAGTACAA GCCCAGTTTC TAAAAAAGGC
    CCGCCCCTCA AGACTCCAGC CTCCAAAAGC CCTAGTGAAG GTCAGCCGGC TACCACCTCT
    CCCAGAGGAA CCAAGCCATC AGTGAAGTCA GAATTAAGCC CTGTTACAAG GCAGGCATCC
    CAGACAGCAG GATCAAACAA AGGACCTTCT AGATCAGGAT CTAGAGATTC CACTCCTTCA
    AGACCTGCCC AGCAACCATT AAGTAGACCA ATGCAGTCTC CAGGGCGAAA CTCAATCTCT
    CCTGGTAGAA ATGGAATAAG TCCCCCTAAC AAATTATCTC AACTACCAAG GACGTCATCC
    CCTAGTACTG CTTCAACTAA GTCCTCAGGT TCTGGGAAAA TGTCTTACAC ATCTCCTGGC
    AGACAGATGA GCCAGCAGAA CCTCACCAAA CAAACAGGCT TATCCAAGAA TGGCAGTGGT
    ATCCCAAGAA GTGAATCTGC CTCCAAAGGG CTAAATCAAA TGAGTAATAG TAATGGATCC
    AATAAAAAAG TAGAACTTTC TAGAATGTCT TCAACAAAGT CAAGTGGAAG TGAATCCGAT
    AGGTCAGAGA GACCTGTATT AGTACGCCAA TCAACTTTCA TCAAAGAAGC TCCAAGCCCA
    ACCCTAAGGA GAAAACTGGA GGAATCTGCT TCATTTGAAT CTCTTTCTCC ATCTTCCAGA
    CCCGATTCTC CCACACGGTC CCAGGCTCAT ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT
    ATGTCTCTAT CTACACATTC GTCTCTTCAG TCTGGTGGAT GGCGAAAACT CCCACCTAAC
    CTCAGTCCCA CCATAGAGTA TAATGATGGA AGACCAGTAA AGCGCCATGA TATAGCACGC
    TCTCATTCCG AAAGTCCTTC CAGACTTCCC ATCAATAGGT CAGGGACCTG GAAACGTGAG
    CACAGCAAAC ACTCATCATC ACTTCCTCGA GTAAGCACTT GGAGAAGAAC TGGAAGTTCA
    TCCTCAATTC TTTCTGCTTC ATCAGAATCT AGTGAAAAGG CAAAAAGTGA GGACGAAAAA
    CAAGTGAACT CTATTTCAGG AAGCAAACAA ACTAAAGAAA ACCAGGTATC CACAAAAGGA
    ACATGGAGAA AAATAAAAGA AAGTGAAATT TCTCCCACAA ATAGTACTTC TCAGACCACT
    TCTTCAGGTG CTGCAAATGG TGCTGAATCA AAGACTCTGA TTTATCAAAT GGCACCTGCT
    GTTTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGATT GCCCCATTAA CAACCCTAGA
    TCTGGAAGAT CTCCAACAGG AAATACTCCC CCCGTGATTG ACACTGTTTC AGAAAAGGGA
    AACCCGAACC CTAAAGATTC AAAAGATAAT CAGGGAAAAC AAAATGTGAG CAATGGTAGT
    GCCCCTACAC GCACCATGGG TCTGGAAAAC CGCCTGAATT CCTTTATTCA GGTAGATCCT
    CCAGACCAAA AAGGAACTGA GACAAAACCG GGACACAGTA ATAACCCTGT CCCTGCATCC
    GAGACTAGTG AAAGTTCTAT AGCCGAGCGT ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG
    CACAGTTCAC CAAGTGGGAC TGTCGCCGCC AGGGTGAGTC CTTTTAATTA CAACCCAAGC
    CCAAGGAAGA GCAGCACAGA TGGCACTTCA GCCCGACCGT CTCAGATCCC AACGCCAGTG
    AGCAACAACA CAAAGAAACG CGACTCAAAA CCCGACAGCA CAGAACCCAG CGGGACTCAA
    AGTCCTAAAC GCCATTCTGG GTCTTACCTT GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328469.1
    CDS142..8826
    Translation

    Target ORF information:

    RefSeq Version XM_015472983.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA
    TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG
    TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC
    CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTGTTGTTGG GAAATTCCCG GGGCAGTAAA
    GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC
    AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT
    GAAACCTGTT GGGAGTGGCA GGAAGCCCAT GAACAAGGGA TGGACCAGGA CAAAAATCCA
    ATGCCAGCTC CTGTTGAACA TCAGATCTGC CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG
    TTTGATGAAG AGCATAGACA TGCCATGAAT GAGCTTGGGG GACTACAGGC CATTGCAGAA
    TTATTGCAAG TGGACTGTGA AATGTATGGG CTTACTAATG ACCACTACAG TATTACTTTA
    AGACGATATG CAGGAATGGC TTTGACAAAC TTGACTTTCG GAGATGTAGC CAACAAGGCT
    ACTCTCTGCT CTATGAAAGG CTGCATGAGA GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC
    GAGGACTTGC AGCAGGTTAT TGCGAGTGTT TTGAGGAATC TGTCTTGGAG AGCAGATGTA
    AATAGTAAAA AGACTTTGCG TGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG
    GAAGTGAAAA AGGAATCAAC CCTCAAAAGT GTATTGAGTG CCTTATGGAA CTTGTCAGCA
    CACTGCACTG AGAATAAAGC TGACATATGT GCTGTGGATG GTGCGCTTGC ATTTTTGGTT
    GGCACTCTCA CTTATCGCAG CCAGACCAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGT
    ATATTACGGA ATGTATCCAG CTTGATAGCT ACAAATGAGG ACCACAGGCA AATCCTAAGA
    GAGAATAATT GCTTACAGAC CTTATTACAA CACTTGAAAT CTCACAGTTT GACAATAGTC
    AGCAATGCAT GCGGAACCTT GTGGAATCTC TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA
    TTGTGGGACA TGGGAGCAGT CAGCATGCTC AAGAACCTCA TTCATTCAAA GCACAAGATG
    ATTGCTATGG GAAGTGCTGC AGCTTTAAGG AATCTCATGG CAAATAGACC TGCAAAGTAT
    AAAGATGCCA ATATCATGTC TCCTGGTTCA AGTTTGCCTT CTCTACATGT CAGGAAACAA
    AAGGCCCTGG AAGCAGAATT AGATGCTCAG CATTTATCAG AAACTTTTGA CAATATTGAC
    AATTTAAGTC CCAAGGCATC TCATCGTAGC AAGCAGAGAC ACAAGCAAAA TCTCTATGGT
    GACTATGTTT TTGACACCAA TCGACATGAT GATAACAGGT CAGACAATTT TAATACTGGA
    AACATGACTG TCCTATCACC GTACTTAAAC ACTACAGTAT TGCCCAGCTC TTCTTCATCA
    AGGGGAAGTT TAGATAGCTC TCGTTCTGAG AAAGATAGAA GTCTGGAGAG AGAACGAGGT
    ATTAGCCTAG GCAACTATCA CCCAGCAACA GAAAACCCAG GAACCTCTTC AAAGCGAGGT
    TTGCAGATTT CTACCACTGC AGCCCAGATT GCCAAAGTCA TGGAAGAAGT ATCAGCTATT
    CATACCTCTC AGGAAGACAG AAGTTCTGGG TCTACCACAG AACTACACTG TGGGACAGAT
    GAGAGGAATG CACTAAGAAG AAGCTCTACC ACCCACACAC ATGCAAACAC GTACAACTTT
    ACCAAGTCAG AAAACTCAAA CAGAACATGT CCAATACCAT ATGCCAAAGT AGAATATAAG
    AGATCTTCAA ATGATAGTTT AAATAGTGTC AGCAGTAGTG ATGGTTATGG TAAAAGAGGT
    CAGATGAAAC CTTCAATTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGCTAT
    GGTCAGTATC CAGCTGACCT AGCCCATAAA ATACATAGTG CAAATCATAT GGATGATAAT
    GATGGAGAAC TAGATACACC AATAAATTAC AGTCTCAAAT ATTCCGATGA ACAGTTGAAC
    TCTGGGAGGC AGAGCCCTTC ACAGAATGAA AGATGGGCAA GACCCAAACA TATACTAGAA
    GATGAAATAA AACCGAATGA GCAGAGACAA TCAAGGAGTC AAAGCACAGC TTATCCCGTG
    TATCCTGAGA GCACTGATGA TAAACACCTC AAGTTCCAAC CACACTTTGG GCAGCAAGAA
    TGTGTTTCCC CATACAGGTC AAGAGCAGCC AATGGATCTG AAACAAATCG AGTAGGCTCT
    AATCATGGAA TTAGTCAAAA TGTGAACCAG TCTTTGTGTC AAGAAGATGA CTATGAAGAC
    GATAAACCAA CCAACTATAG CGAACGTTAC TCTGAGGAAG GGCAGCATGA GGAAGAAGAG
    AGGCCAACCA ATTATAGCAT AAAATACAGT GAAGAAAAAC ATCACGTGGA TCAGCCTATT
    GATTATAGTT TAAAATACAC CACAGACATT CCTTCTTCAC AGAAACCAGC ATTTTCATTC
    TCAAAGAATT CATCTGGACA GAGCACAAAA ACTGAACACA TCTCTTCAAG CAGCGAGAAT
    ACATCCACAA CTTCATCTAA TGCCAAGAGG CAGAATCAGC TGCATCCAAG CTCAGCACAG
    AGCAGAAGTG GTCAGACCCC AAAAGCCACC TCTTCCTCTT GCAAAGTCCC CTCTATCAAC
    CAAGAAACAA TACAGACTTA CTGTGTAGAA GATACCCCAA TATGTTTTTC AAGATGCAGT
    TCATTATCAT CTCTGTCATC TGCTGAAGAT GAAGTAGGGT GTGATCAGAC AACACAAGAA
    GCAGAGTCTG CTAACACTCT GCAAATAGCT GAAATCAAGG ACAACAGCGG ACCTAGGTCA
    AATGAAGATT CTGTGAGTAA AGTTCCAGCA GGGTCACAGC ACATTAGAAC CAAATCCAGC
    AGACTCCAGG CTTCTGGTCT GTCTTCAGAG TCAGCCAGGC ACAAAGCTGT TGAATTTTCT
    TCAGGGGCCA AATCTCCTTC CAAGAGCGGT GCTCAGACAC CCAAAAGTCC ACCAGAGCAC
    TACGTTCAGG AGACCCCACT CATGTTTAGC AGATGTACTT CAGTCAGTTC ACTCGACAGT
    TTTGAGAGTC GCTCGATTGC CAGCTCTGTT CAGAGTGAAC CCTGCAGTGG AATGGTAAGT
    GGCATCATAA GCCCCAGTGA CCTCCCAGAT AGCCCTGGAC AAACCATGCC ACCAAGCAGA
    AGCAAAACCC CTCCGCCGCC GCCTCCGCCT CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA
    GTACCTAAAA ATAAAGCACC TAGTGCTGAA AAGAGAGAAA GTGGACCCAA GCAAGCTGCT
    GTAAATGCTG CAGTACAAAG GGTCCAGGTT CTTCCAGAGG CTGATACTCT GTTACATTTT
    GCCACAGAGA GTACTCCTGA TGGATTTTCT TGTTCATCTA GCCTGAGCGC TCTGAGCCTT
    GATGAGCCAT TTATTCAGAA AGATGTGGAA CTAAGAATAA TGCCTCCAGT TCAGGAAAAT
    GACAACGGGA ATGAAACAGA AAGTGAGCAG CCTGAAGAAT CAAATGAAAA CCAGGAAAAA
    GAGGCAGAAA AACCCACTGA TTCTGAAAAA GATCTTTTAG ATGAGTCAGA TGATGATGAT
    ATTGAAATAC TAGAAGAGTG TATAATTTCT GCCATGCCAA CAAAATCTTC ACGCAAAGCC
    AAAAAACCAG CCCAGACTAC TTCAAAATTA CCTCCACCTG TGGCAAGGAA ACCAAGTCAG
    CTCCCTGTAT ACAAACTTCT GCCATCGCAA AACAGGTTAC AGGCACAAAA GCATGTTAGT
    TTTACACCAG GAGATGATAT GCCACGGGTG TATTGTGTAG AAGGGACACC TATAAACTTT
    TCCACAGCTA CATCTCTGAG TGATCTAACG ATAGAATCCC CTCCAAATGA GTTAGCTGCT
    GGAGAAGGGG TTAGAGCAGG GGCACAGTCA AGTGAGTTTG AAAAACGAGA TACCATTCCT
    ACTGAAGGCA GAAGTACAGA TGAGGCTCAA CGAGGGAAAG CCTCATCTGT CACTGTGCCT
    GAACTGGACG ACAGCAAAAC AGAAGAAGGT GATATTCTTG CAGAATGCAT TAATTCTGCC
    ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC CGTGTGAAAA AGATCATGGA CCAGGTCCAG
    CAAGCATCTA TGTCTTCATC TGGAACTAAC AAAAATCAAT TAGATGGTAA GACGAAGAAA
    CCTACTTCAC CAGTAAAACC TATACCACAA AATACTGAAT ACAGGACACG TGTAAGAAAA
    AATACAGACT CAAAAAATAA TCTAAATGCA GAAAGAAATT TCTCGGAAAA CAAAGACTCA
    AAGAAACAGC ACTTGAAAAA TAATTCCAAG GACTTCAATG ATAAACTGCC AAATAATGAA
    GACAGAGTCA GAGGAAGTTT TACTTTTGAT TCACCCCATC ATTACACGCC CATTGAAGGC
    ACTCCGTACT GTTTTTCACG AAATGACTCT CTGAGTTCTC TAGATTTTGA TGATGATGAT
    GTTGACCTTT CCAGGGAAAA GGCTGAATTA AGAAAGGGGA AGGAAAATAA AGAATCAGAA
    GCTAAAGTGA CCAACCACAC AGAACTAACC TCAAACCAAC AATCAGCTAG TAAGACACCA
    GCTGTTACAA AGCAGCCAAT AAATAGAGGT CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC
    ACTTTTCCCC AGTCTTCCAA AGATATACCA GACAGAGGGG CAGCAACAGA TGAGAAATTA
    CAGAATTTCG CTATTGAAAA TACTCCAGTT TGCTTTTCTC GAAATTCCTC TCTAAGCTCT
    CTTAGTGACA TTGATCAAGA AAACAACAAC AACAAGGAAA ATGAACCTGT CAAAGAGACA
    GAGCCCCCTG CCTCACAGGG AGAACCAGGT AAACCCCAGG CCTCAGGTTA TGCTCCTAAA
    TCGTTTCACG TGGAAGACAC CCCTGTTTGT TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT
    AGTATTGATT CTGAAGATGA CCTGCTGCAG GAATGTATAA GTTCTGCAAT GCCAAAAAAG
    AAAAAGCCTT CAAGGCTCAA GCCCGATAAT GAAAAGCATA GTCCCAGAAA TATGGGTGGC
    ATATTAGCAG AAGATTTGAC ACTCGATTTG AAAGATATAC AGAGACCAGA TTCAGAACAT
    GGTTTATCCC CAGATTCAGA AAATTTTGAT TGGAAAGCTA TTCAGGAAGG TGCAAATTCC
    ATAGTAAGTA GTTTACATCA AGCTGCTGCC GCTGCATGTT TATCTCGACA AGCTTCATCT
    GATTCAGATT CTATCCTTTC ATTGAAATCG GGTATCTCTC TGGGATCACC ATTTCACCTT
    ACACCTGATC AAGAAGAAAA ACCCTTTACG AGTAATAAAG GTCCACGAAT TCTAAAACCT
    GGGGAGAAAA GTACATTGGA AACTAAAAAA ATAGAATCTG AAAATAAAGG AATAAAAGGA
    GGCAAAAAGG TTTATAAAAG TTTGATTACT GGAAAAGTTC GATCTAATTC GGAAATTTCG
    AGCCAAATGA AACAACCCCT TCAAACAAAC ATGCCTTCAA TCTCTCGAGG TAGGACAATG
    ATTCATATTC CAGGAGTTCG GAATAGCTCT TCAAGTACAA GCCCAGTTTC TAAAAAAGGC
    CCGCCCCTCA AGACTCCAGC CTCCAAAAGC CCTAGTGAAG GTCAGCCGGC TACCACCTCT
    CCCAGAGGAA CCAAGCCATC AGTGAAGTCA GAATTAAGCC CTGTTACAAG GCAGGCATCC
    CAGACAGCAG GATCAAACAA AGGACCTTCT AGATCAGGAT CTAGAGATTC CACTCCTTCA
    AGACCTGCCC AGCAACCATT AAGTAGACCA ATGCAGTCTC CAGGGCGAAA CTCAATCTCT
    CCTGGTAGAA ATGGAATAAG TCCCCCTAAC AAATTATCTC AACTACCAAG GACGTCATCC
    CCTAGTACTG CTTCAACTAA GTCCTCAGGT TCTGGGAAAA TGTCTTACAC ATCTCCTGGC
    AGACAGATGA GCCAGCAGAA CCTCACCAAA CAAACAGGCT TATCCAAGAA TGGCAGTGGT
    ATCCCAAGAA GTGAATCTGC CTCCAAAGGG CTAAATCAAA TGAGTAATAG TAATGGATCC
    AATAAAAAAG TAGAACTTTC TAGAATGTCT TCAACAAAGT CAAGTGGAAG TGAATCCGAT
    AGGTCAGAGA GACCTGTATT AGTACGCCAA TCAACTTTCA TCAAAGAAGC TCCAAGCCCA
    ACCCTAAGGA GAAAACTGGA GGAATCTGCT TCATTTGAAT CTCTTTCTCC ATCTTCCAGA
    CCCGATTCTC CCACACGGTC CCAGGCTCAT ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT
    ATGTCTCTAT CTACACATTC GTCTCTTCAG TCTGGTGGAT GGCGAAAACT CCCACCTAAC
    CTCAGTCCCA CCATAGAGTA TAATGATGGA AGACCAGTAA AGCGCCATGA TATAGCACGC
    TCTCATTCCG AAAGTCCTTC CAGACTTCCC ATCAATAGGT CAGGGACCTG GAAACGTGAG
    CACAGCAAAC ACTCATCATC ACTTCCTCGA GTAAGCACTT GGAGAAGAAC TGGAAGTTCA
    TCCTCAATTC TTTCTGCTTC ATCAGAATCT AGTGAAAAGG CAAAAAGTGA GGACGAAAAA
    CAAGTGAACT CTATTTCAGG AAGCAAACAA ACTAAAGAAA ACCAGGTATC CACAAAAGGA
    ACATGGAGAA AAATAAAAGA AAGTGAAATT TCTCCCACAA ATAGTACTTC TCAGACCACT
    TCTTCAGGTG CTGCAAATGG TGCTGAATCA AAGACTCTGA TTTATCAAAT GGCACCTGCT
    GTTTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGATT GCCCCATTAA CAACCCTAGA
    TCTGGAAGAT CTCCAACAGG AAATACTCCC CCCGTGATTG ACACTGTTTC AGAAAAGGGA
    AACCCGAACC CTAAAGATTC AAAAGATAAT CAGGGAAAAC AAAATGTGAG CAATGGTAGT
    GCCCCTACAC GCACCATGGG TCTGGAAAAC CGCCTGAATT CCTTTATTCA GGTAGATCCT
    CCAGACCAAA AAGGAACTGA GACAAAACCG GGACACAGTA ATAACCCTGT CCCTGCATCC
    GAGACTAGTG AAAGTTCTAT AGCCGAGCGT ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG
    CACAGTTCAC CAAGTGGGAC TGTCGCCGCC AGGGTGAGTC CTTTTAATTA CAACCCAAGC
    CCAAGGAAGA GCAGCACAGA TGGCACTTCA GCCCGACCGT CTCAGATCCC AACGCCAGTG
    AGCAACAACA CAAAGAAACG CGACTCAAAA CCCGACAGCA CAGAACCCAG CGGGACTCAA
    AGTCCTAAAC GCCATTCTGG GTCTTACCTT GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91578
    Clone ID Related Accession (Same CDS sequence) XM_015472984.1
    Accession Version XM_015472984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGTGGCTGT ATTGGCGCAG CCCGCCTGGG TGTCACTGGA GACGGGATGG AGGTGCTGCT 
    GGGCTCGGAA ATGGGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328470.1
    CDS16..8664
    Translation

    Target ORF information:

    RefSeq Version XM_015472984.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGTGGCTGT ATTGGCGCAG CCCGCCTGGG TGTCACTGGA GACGGGATGG AGGTGCTGCT 
    GGGCTCGGAA ATGGGGAAGT ACTTAAACAA CTACAAGGAA GTATTGAAGA TGAAGCTATG
    GCTTCTTCTG GACAGATTGA TTTATTAGAG CGTCTTAAAG AACTTAACTT AGATAGCAGT
    AATTTCCCTG GAGTGAAACT ACGGTCCAAA ATGTCCCTCC GTTCTTACGG AAGCCGAGAA
    GGATCTGTAT CTAGTCGCTC AGGAGAGTGC AGTCCTGTTC CTATGGGTTC ATTTCCAAGA
    AGAGGGTTTG TAAATGGAAG CAGAGAAAAT ACCGGTTATT TAGAAGAACT TGAAAAAGAG
    AGATCATTGC TTCTTGCTGA CCTTGACAAA GAAGAAAAGG AAAAAGACTG GTATTATGCT
    CAACTTCAGA ATCTCACTAA AAGAATAGAT AGCCTTCCTT TAACTGAAAA TTTTTCCTTA
    CAAACAGATA TGACCAGAAG GCAGTTGGAA TATGAGGCAA GGCAAATCAG AGTTGCAATG
    GAAGAACAAC TAGGTACTTG CCAGGATATG GAAAAACGAG CACAGCGAAG AATAACCAGA
    ATTCAACAAA TAGAAAAGGA CATACTTCGT ATACGACAGC TTTTACAGTC CCAAGCAACA
    GAAGCAGAGA GGTCATCTCA GAGCAAGCAT GAAGCGGGCT CACATGAAGC TGAACGGCAG
    AATGAAGGTC AAGGAGTGGC AGAAATCAAC ATGGCAACCT CGGGTAGCGG TCAGGTTACT
    GCTTCTCTGG TCCCTCTGAA GGATGTCGGT GCAGACTTAA TCATACTAAA AAGGCCCAGG
    AAAGATTCTC AAGACTCGGG TTCTGGCTTA CAGGGTTCAA CTACACGAAT AGATCATGAA
    ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CATTCTGCTC CTCGAAGGCT GACGAGTCAT
    CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG
    GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG
    CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT
    GTGTTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC
    CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT
    CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAT
    GAACAAGGGA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGC
    CCTGCTGTCT GTGTTCTAAT GAAGCTTTCG TTTGATGAAG AGCATAGACA TGCCATGAAT
    GAGCTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGGCA GGGGCTTTCA
    GGGGGACTAC AGGCCATTGC AGAATTATTG CAAGTGGACT GTGAAATGTA TGGGCTTACT
    AATGACCACT ACAGTATTAC TTTAAGACGA TATGCAGGAA TGGCTTTGAC AAACTTGACT
    TTCGGAGATG TAGCCAACAA GGCTACTCTC TGCTCTATGA AAGGCTGCAT GAGAGCGCTC
    GTGGCTCAGC TCCAGTCTGA AAGCGAGGAC TTGCAGCAGG TTATTGCGAG TGTTTTGAGG
    AATCTGTCTT GGAGAGCAGA TGTAAATAGT AAAAAGACTT TGCGTGAAGT TGGAAGTGTG
    AAAGCATTGA TGGAATGTGC TTTGGAAGTG AAAAAGGAAT CAACCCTCAA AAGTGTATTG
    AGTGCCTTAT GGAACTTGTC AGCACACTGC ACTGAGAATA AAGCTGACAT ATGTGCTGTG
    GATGGTGCGC TTGCATTTTT GGTTGGCACT CTCACTTATC GCAGCCAGAC CAATACTTTA
    GCCATTATTG AAAGTGGAGG TGGTATATTA CGGAATGTAT CCAGCTTGAT AGCTACAAAT
    GAGGACCACA GGCAAATCCT AAGAGAGAAT AATTGCTTAC AGACCTTATT ACAACACTTG
    AAATCTCACA GTTTGACAAT AGTCAGCAAT GCATGCGGAA CCTTGTGGAA TCTCTCAGCA
    AGAAACCCTA AAGACCAGGA AGCATTGTGG GACATGGGAG CAGTCAGCAT GCTCAAGAAC
    CTCATTCATT CAAAGCACAA GATGATTGCT ATGGGAAGTG CTGCAGCTTT AAGGAATCTC
    ATGGCAAATA GACCTGCAAA GTATAAAGAT GCCAATATCA TGTCTCCTGG TTCAAGTTTG
    CCTTCTCTAC ATGTCAGGAA ACAAAAGGCC CTGGAAGCAG AATTAGATGC TCAGCATTTA
    TCAGAAACTT TTGACAATAT TGACAATTTA AGTCCCAAGG CATCTCATCG TAGCAAGCAG
    AGACACAAGC AAAATCTCTA TGGTGACTAT GTTTTTGACA CCAATCGACA TGATGATAAC
    AGGTCAGACA ATTTTAATAC TGGAAACATG ACTGTCCTAT CACCGTACTT AAACACTACA
    GTATTGCCCA GCTCTTCTTC ATCAAGGGGA AGTTTAGATA GCTCTCGTTC TGAGAAAGAT
    AGAAGTCTGG AGAGAGAACG AGGTATTAGC CTAGGCAACT ATCACCCAGC AACAGAAAAC
    CCAGGAACCT CTTCAAAGCG AGGTTTGCAG ATTTCTACCA CTGCAGCCCA GATTGCCAAA
    GTCATGGAAG AAGTATCAGC TATTCATACC TCTCAGGAAG ACAGAAGTTC TGGGTCTACC
    ACAGAACTAC ACTGTGGGAC AGATGAGAGG AATGCACTAA GAAGAAGCTC TACCACCCAC
    ACACATGCAA ACACGTACAA CTTTACCAAG TCAGAAAACT CAAACAGAAC ATGTCCAATA
    CCATATGCCA AAGTAGAATA TAAGAGATCT TCAAATGATA GTTTAAATAG TGTCAGCAGT
    AGTGATGGTT ATGGTAAAAG AGGTCAGATG AAACCTTCAA TTGAATCCTA TTCTGAAGAT
    GATGAAAGTA AATTTTGCAG CTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAAATACAT
    AGTGCAAATC ATATGGATGA TAATGATGGA GAACTAGATA CACCAATAAA TTACAGTCTC
    AAATATTCCG ATGAACAGTT GAACTCTGGG AGGCAGAGCC CTTCACAGAA TGAAAGATGG
    GCAAGACCCA AACATATACT AGAAGATGAA ATAAAACCGA ATGAGCAGAG ACAATCAAGG
    AGTCAAAGCA CAGCTTATCC CGTGTATCCT GAGAGCACTG ATGATAAACA CCTCAAGTTC
    CAACCACACT TTGGGCAGCA AGAATGTGTT TCCCCATACA GGTCAAGAGC AGCCAATGGA
    TCTGAAACAA ATCGAGTAGG CTCTAATCAT GGAATTAGTC AAAATGTGAA CCAGTCTTTG
    TGTCAAGAAG ATGACTATGA AGACGATAAA CCAACCAACT ATAGCGAACG TTACTCTGAG
    GAAGGGCAGC ATGAGGAAGA AGAGAGGCCA ACCAATTATA GCATAAAATA CAGTGAAGAA
    AAACATCACG TGGATCAGCC TATTGATTAT AGTTTAAAAT ACACCACAGA CATTCCTTCT
    TCACAGAAAC CAGCATTTTC ATTCTCAAAG AATTCATCTG GACAGAGCAC AAAAACTGAA
    CACATCTCTT CAAGCAGCGA GAATACATCC ACAACTTCAT CTAATGCCAA GAGGCAGAAT
    CAGCTGCATC CAAGCTCAGC ACAGAGCAGA AGTGGTCAGA CCCCAAAAGC CACCTCTTCC
    TCTTGCAAAG TCCCCTCTAT CAACCAAGAA ACAATACAGA CTTACTGTGT AGAAGATACC
    CCAATATGTT TTTCAAGATG CAGTTCATTA TCATCTCTGT CATCTGCTGA AGATGAAGTA
    GGGTGTGATC AGACAACACA AGAAGCAGAG TCTGCTAACA CTCTGCAAAT AGCTGAAATC
    AAGGACAACA GCGGACCTAG GTCAAATGAA GATTCTGTGA GTAAAGTTCC AGCAGGGTCA
    CAGCACATTA GAACCAAATC CAGCAGACTC CAGGCTTCTG GTCTGTCTTC AGAGTCAGCC
    AGGCACAAAG CTGTTGAATT TTCTTCAGGG GCCAAATCTC CTTCCAAGAG CGGTGCTCAG
    ACACCCAAAA GTCCACCAGA GCACTACGTT CAGGAGACCC CACTCATGTT TAGCAGATGT
    ACTTCAGTCA GTTCACTCGA CAGTTTTGAG AGTCGCTCGA TTGCCAGCTC TGTTCAGAGT
    GAACCCTGCA GTGGAATGGT AAGTGGCATC ATAAGCCCCA GTGACCTCCC AGATAGCCCT
    GGACAAACCA TGCCACCAAG CAGAAGCAAA ACCCCTCCGC CGCCGCCTCC GCCTCCTCCT
    CAGACAGTTC AAACGAAGCA GGAAGTACCT AAAAATAAAG CACCTAGTGC TGAAAAGAGA
    GAAAGTGGAC CCAAGCAAGC TGCTGTAAAT GCTGCAGTAC AAAGGGTCCA GGTTCTTCCA
    GAGGCTGATA CTCTGTTACA TTTTGCCACA GAGAGTACTC CTGATGGATT TTCTTGTTCA
    TCTAGCCTGA GCGCTCTGAG CCTTGATGAG CCATTTATTC AGAAAGATGT GGAACTAAGA
    ATAATGCCTC CAGTTCAGGA AAATGACAAC GGGAATGAAA CAGAAAGTGA GCAGCCTGAA
    GAATCAAATG AAAACCAGGA AAAAGAGGCA GAAAAACCCA CTGATTCTGA AAAAGATCTT
    TTAGATGAGT CAGATGATGA TGATATTGAA ATACTAGAAG AGTGTATAAT TTCTGCCATG
    CCAACAAAAT CTTCACGCAA AGCCAAAAAA CCAGCCCAGA CTACTTCAAA ATTACCTCCA
    CCTGTGGCAA GGAAACCAAG TCAGCTCCCT GTATACAAAC TTCTGCCATC GCAAAACAGG
    TTACAGGCAC AAAAGCATGT TAGTTTTACA CCAGGAGATG ATATGCCACG GGTGTATTGT
    GTAGAAGGGA CACCTATAAA CTTTTCCACA GCTACATCTC TGAGTGATCT AACGATAGAA
    TCCCCTCCAA ATGAGTTAGC TGCTGGAGAA GGGGTTAGAG CAGGGGCACA GTCAAGTGAG
    TTTGAAAAAC GAGATACCAT TCCTACTGAA GGCAGAAGTA CAGATGAGGC TCAACGAGGG
    AAAGCCTCAT CTGTCACTGT GCCTGAACTG GACGACAGCA AAACAGAAGA AGGTGATATT
    CTTGCAGAAT GCATTAATTC TGCCATGCCC AAAGGAAAAA GTCACAAGCC TTTCCGTGTG
    AAAAAGATCA TGGACCAGGT CCAGCAAGCA TCTATGTCTT CATCTGGAAC TAACAAAAAT
    CAATTAGATG GTAAGACGAA GAAACCTACT TCACCAGTAA AACCTATACC ACAAAATACT
    GAATACAGGA CACGTGTAAG AAAAAATACA GACTCAAAAA ATAATCTAAA TGCAGAAAGA
    AATTTCTCGG AAAACAAAGA CTCAAAGAAA CAGCACTTGA AAAATAATTC CAAGGACTTC
    AATGATAAAC TGCCAAATAA TGAAGACAGA GTCAGAGGAA GTTTTACTTT TGATTCACCC
    CATCATTACA CGCCCATTGA AGGCACTCCG TACTGTTTTT CACGAAATGA CTCTCTGAGT
    TCTCTAGATT TTGATGATGA TGATGTTGAC CTTTCCAGGG AAAAGGCTGA ATTAAGAAAG
    GGGAAGGAAA ATAAAGAATC AGAAGCTAAA GTGACCAACC ACACAGAACT AACCTCAAAC
    CAACAATCAG CTAGTAAGAC ACCAGCTGTT ACAAAGCAGC CAATAAATAG AGGTCAGTCT
    AAACCCGTGC TGCAGAAGCA GTCCACTTTT CCCCAGTCTT CCAAAGATAT ACCAGACAGA
    GGGGCAGCAA CAGATGAGAA ATTACAGAAT TTCGCTATTG AAAATACTCC AGTTTGCTTT
    TCTCGAAATT CCTCTCTAAG CTCTCTTAGT GACATTGATC AAGAAAACAA CAACAACAAG
    GAAAATGAAC CTGTCAAAGA GACAGAGCCC CCTGCCTCAC AGGGAGAACC AGGTAAACCC
    CAGGCCTCAG GTTATGCTCC TAAATCGTTT CACGTGGAAG ACACCCCTGT TTGTTTCTCA
    AGAAACAGTT CTCTCAGTTC TCTTAGTATT GATTCTGAAG ATGACCTGCT GCAGGAATGT
    ATAAGTTCTG CAATGCCAAA AAAGAAAAAG CCTTCAAGGC TCAAGCCCGA TAATGAAAAG
    CATAGTCCCA GAAATATGGG TGGCATATTA GCAGAAGATT TGACACTCGA TTTGAAAGAT
    ATACAGAGAC CAGATTCAGA ACATGGTTTA TCCCCAGATT CAGAAAATTT TGATTGGAAA
    GCTATTCAGG AAGGTGCAAA TTCCATAGTA AGTAGTTTAC ATCAAGCTGC TGCCGCTGCA
    TGTTTATCTC GACAAGCTTC ATCTGATTCA GATTCTATCC TTTCATTGAA ATCGGGTATC
    TCTCTGGGAT CACCATTTCA CCTTACACCT GATCAAGAAG AAAAACCCTT TACGAGTAAT
    AAAGGTCCAC GAATTCTAAA ACCTGGGGAG AAAAGTACAT TGGAAACTAA AAAAATAGAA
    TCTGAAAATA AAGGAATAAA AGGAGGCAAA AAGGTTTATA AAAGTTTGAT TACTGGAAAA
    GTTCGATCTA ATTCGGAAAT TTCGAGCCAA ATGAAACAAC CCCTTCAAAC AAACATGCCT
    TCAATCTCTC GAGGTAGGAC AATGATTCAT ATTCCAGGAG TTCGGAATAG CTCTTCAAGT
    ACAAGCCCAG TTTCTAAAAA AGGCCCGCCC CTCAAGACTC CAGCCTCCAA AAGCCCTAGT
    GAAGGTCAGC CGGCTACCAC CTCTCCCAGA GGAACCAAGC CATCAGTGAA GTCAGAATTA
    AGCCCTGTTA CAAGGCAGGC ATCCCAGACA GCAGGATCAA ACAAAGGACC TTCTAGATCA
    GGATCTAGAG ATTCCACTCC TTCAAGACCT GCCCAGCAAC CATTAAGTAG ACCAATGCAG
    TCTCCAGGGC GAAACTCAAT CTCTCCTGGT AGAAATGGAA TAAGTCCCCC TAACAAATTA
    TCTCAACTAC CAAGGACGTC ATCCCCTAGT ACTGCTTCAA CTAAGTCCTC AGGTTCTGGG
    AAAATGTCTT ACACATCTCC TGGCAGACAG ATGAGCCAGC AGAACCTCAC CAAACAAACA
    GGCTTATCCA AGAATGGCAG TGGTATCCCA AGAAGTGAAT CTGCCTCCAA AGGGCTAAAT
    CAAATGAGTA ATAGTAATGG ATCCAATAAA AAAGTAGAAC TTTCTAGAAT GTCTTCAACA
    AAGTCAAGTG GAAGTGAATC CGATAGGTCA GAGAGACCTG TATTAGTACG CCAATCAACT
    TTCATCAAAG AAGCTCCAAG CCCAACCCTA AGGAGAAAAC TGGAGGAATC TGCTTCATTT
    GAATCTCTTT CTCCATCTTC CAGACCCGAT TCTCCCACAC GGTCCCAGGC TCATACTCCA
    GTTTTAAGTC CTTCCCTTCC TGATATGTCT CTATCTACAC ATTCGTCTCT TCAGTCTGGT
    GGATGGCGAA AACTCCCACC TAACCTCAGT CCCACCATAG AGTATAATGA TGGAAGACCA
    GTAAAGCGCC ATGATATAGC ACGCTCTCAT TCCGAAAGTC CTTCCAGACT TCCCATCAAT
    AGGTCAGGGA CCTGGAAACG TGAGCACAGC AAACACTCAT CATCACTTCC TCGAGTAAGC
    ACTTGGAGAA GAACTGGAAG TTCATCCTCA ATTCTTTCTG CTTCATCAGA ATCTAGTGAA
    AAGGCAAAAA GTGAGGACGA AAAACAAGTG AACTCTATTT CAGGAAGCAA ACAAACTAAA
    GAAAACCAGG TATCCACAAA AGGAACATGG AGAAAAATAA AAGAAAGTGA AATTTCTCCC
    ACAAATAGTA CTTCTCAGAC CACTTCTTCA GGTGCTGCAA ATGGTGCTGA ATCAAAGACT
    CTGATTTATC AAATGGCACC TGCTGTTTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG
    GATTGCCCCA TTAACAACCC TAGATCTGGA AGATCTCCAA CAGGAAATAC TCCCCCCGTG
    ATTGACACTG TTTCAGAAAA GGGAAACCCG AACCCTAAAG ATTCAAAAGA TAATCAGGGA
    AAACAAAATG TGAGCAATGG TAGTGCCCCT ACACGCACCA TGGGTCTGGA AAACCGCCTG
    AATTCCTTTA TTCAGGTAGA TCCTCCAGAC CAAAAAGGAA CTGAGACAAA ACCGGGACAC
    AGTAATAACC CTGTCCCTGC ATCCGAGACT AGTGAAAGTT CTATAGCCGA GCGTACCCCG
    TTTAGTTCTA GCAGCTCAAG CAAGCACAGT TCACCAAGTG GGACTGTCGC CGCCAGGGTG
    AGTCCTTTTA ATTACAACCC AAGCCCAAGG AAGAGCAGCA CAGATGGCAC TTCAGCCCGA
    CCGTCTCAGA TCCCAACGCC AGTGAGCAAC AACACAAAGA AACGCGACTC AAAACCCGAC
    AGCACAGAAC CCAGCGGGAC TCAAAGTCCT AAACGCCATT CTGGGTCTTA CCTTGTGACA
    TCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91579
    Clone ID Related Accession (Same CDS sequence) XM_015472986.1
    Accession Version XM_015472986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGATACGAGC TTACTGTGAA
    ACCTGTTGGG AGTGGCAGGA AGCCCATGAA CAAGGGATGG ACCAGGACAA AAATCCAATG
    CCAGCTCCTG TTGAACATCA GATCTGCCCT GCTGTCTGTG TTCTAATGAA GCTTTCGTTT
    GATGAAGAGC ATAGACATGC CATGAATGAG CTTGGTAGGA AGGCTACCCG GGGCATTTCA
    TCCCAGGAGC TAGGGCAGGG GCTTTCAGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328472.1
    CDS142..8577
    Translation

    Target ORF information:

    RefSeq Version XM_015472986.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGTACACCC CCCTCTGCTC GAGTGCGGTC GCCGCTTTGC CTGCTTCGGT ACCGCCCTGT 
    GCTGTCCGGT CCCGGAGCTC CGGCGGCGGC AGGGGCTGTG TCCGGCAGGA GAGGAAGCGC
    CCGGGCTGCG TCCGTGCTTC TGTCCGCGGG GCGAGCGTCT GGCAGGAAGT ACTTAAACAA
    CTACAAGGAA GTATTGAAGA TGAAGCTATG GCTTCTTCTG GACAGATTGA TTTATTAGAG
    CGTCTTAAAG AACTTAACTT AGATAGCAGT AATTTCCCTG GAGTGAAACT ACGGTCCAAA
    ATGTCCCTCC GTTCTTACGG AAGCCGAGAA GGATCTGTAT CTAGTCGCTC AGGAGAGTGC
    AGTCCTGTTC CTATGGGTTC ATTTCCAAGA AGAGGGTTTG TAAATGGAAG CAGAGAAAAT
    ACCGGTTATT TAGAAGAACT TGAAAAAGAG AGATCATTGC TTCTTGCTGA CCTTGACAAA
    GAAGAAAAGG AAAAAGACTG GTATTATGCT CAACTTCAGA ATCTCACTAA AAGAATAGAT
    AGCCTTCCTT TAACTGAAAA TTTTTCCTTA CAAACAGATA TGACCAGAAG GCAGTTGGAA
    TATGAGGCAA GGCAAATCAG AGTTGCAATG GAAGAACAAC TAGGTACTTG CCAGGATATG
    GAAAAACGAG CACAGCGAAG AATAACCAGA ATTCAACAAA TAGAAAAGGA CATACTTCGT
    ATACGACAGC TTTTACAGTC CCAAGCAACA GAAGCAGAGA GGTCATCTCA GAGCAAGCAT
    GAAGCGGGCT CACATGAAGC TGAACGGCAG AATGAAGGTC AAGGAGTGGC AGAAATCAAC
    ATGGCAACCT CGGGTAGCGG TCAGGTTACT GCTTCTCTGG TCCCTCTGAA GGATGTCGGT
    GCAGACTTAA TCATACTAAA AAGGCCCAGG AAAGATTCTC AAGACTCGGG TTCTGGCTTA
    CAGGGTTCAA CTACACGAAT AGATCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA
    CATTCTGCTC CTCGAAGGCT GACGAGTCAT CTGGGAACCA AGATACGAGC TTACTGTGAA
    ACCTGTTGGG AGTGGCAGGA AGCCCATGAA CAAGGGATGG ACCAGGACAA AAATCCAATG
    CCAGCTCCTG TTGAACATCA GATCTGCCCT GCTGTCTGTG TTCTAATGAA GCTTTCGTTT
    GATGAAGAGC ATAGACATGC CATGAATGAG CTTGGTAGGA AGGCTACCCG GGGCATTTCA
    TCCCAGGAGC TAGGGCAGGG GCTTTCAGGG GGACTACAGG CCATTGCAGA ATTATTGCAA
    GTGGACTGTG AAATGTATGG GCTTACTAAT GACCACTACA GTATTACTTT AAGACGATAT
    GCAGGAATGG CTTTGACAAA CTTGACTTTC GGAGATGTAG CCAACAAGGC TACTCTCTGC
    TCTATGAAAG GCTGCATGAG AGCGCTCGTG GCTCAGCTCC AGTCTGAAAG CGAGGACTTG
    CAGCAGGTTA TTGCGAGTGT TTTGAGGAAT CTGTCTTGGA GAGCAGATGT AAATAGTAAA
    AAGACTTTGC GTGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTGAAA
    AAGGAATCAA CCCTCAAAAG TGTATTGAGT GCCTTATGGA ACTTGTCAGC ACACTGCACT
    GAGAATAAAG CTGACATATG TGCTGTGGAT GGTGCGCTTG CATTTTTGGT TGGCACTCTC
    ACTTATCGCA GCCAGACCAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG TATATTACGG
    AATGTATCCA GCTTGATAGC TACAAATGAG GACCACAGGC AAATCCTAAG AGAGAATAAT
    TGCTTACAGA CCTTATTACA ACACTTGAAA TCTCACAGTT TGACAATAGT CAGCAATGCA
    TGCGGAACCT TGTGGAATCT CTCAGCAAGA AACCCTAAAG ACCAGGAAGC ATTGTGGGAC
    ATGGGAGCAG TCAGCATGCT CAAGAACCTC ATTCATTCAA AGCACAAGAT GATTGCTATG
    GGAAGTGCTG CAGCTTTAAG GAATCTCATG GCAAATAGAC CTGCAAAGTA TAAAGATGCC
    AATATCATGT CTCCTGGTTC AAGTTTGCCT TCTCTACATG TCAGGAAACA AAAGGCCCTG
    GAAGCAGAAT TAGATGCTCA GCATTTATCA GAAACTTTTG ACAATATTGA CAATTTAAGT
    CCCAAGGCAT CTCATCGTAG CAAGCAGAGA CACAAGCAAA ATCTCTATGG TGACTATGTT
    TTTGACACCA ATCGACATGA TGATAACAGG TCAGACAATT TTAATACTGG AAACATGACT
    GTCCTATCAC CGTACTTAAA CACTACAGTA TTGCCCAGCT CTTCTTCATC AAGGGGAAGT
    TTAGATAGCT CTCGTTCTGA GAAAGATAGA AGTCTGGAGA GAGAACGAGG TATTAGCCTA
    GGCAACTATC ACCCAGCAAC AGAAAACCCA GGAACCTCTT CAAAGCGAGG TTTGCAGATT
    TCTACCACTG CAGCCCAGAT TGCCAAAGTC ATGGAAGAAG TATCAGCTAT TCATACCTCT
    CAGGAAGACA GAAGTTCTGG GTCTACCACA GAACTACACT GTGGGACAGA TGAGAGGAAT
    GCACTAAGAA GAAGCTCTAC CACCCACACA CATGCAAACA CGTACAACTT TACCAAGTCA
    GAAAACTCAA ACAGAACATG TCCAATACCA TATGCCAAAG TAGAATATAA GAGATCTTCA
    AATGATAGTT TAAATAGTGT CAGCAGTAGT GATGGTTATG GTAAAAGAGG TCAGATGAAA
    CCTTCAATTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGCTA TGGTCAGTAT
    CCAGCTGACC TAGCCCATAA AATACATAGT GCAAATCATA TGGATGATAA TGATGGAGAA
    CTAGATACAC CAATAAATTA CAGTCTCAAA TATTCCGATG AACAGTTGAA CTCTGGGAGG
    CAGAGCCCTT CACAGAATGA AAGATGGGCA AGACCCAAAC ATATACTAGA AGATGAAATA
    AAACCGAATG AGCAGAGACA ATCAAGGAGT CAAAGCACAG CTTATCCCGT GTATCCTGAG
    AGCACTGATG ATAAACACCT CAAGTTCCAA CCACACTTTG GGCAGCAAGA ATGTGTTTCC
    CCATACAGGT CAAGAGCAGC CAATGGATCT GAAACAAATC GAGTAGGCTC TAATCATGGA
    ATTAGTCAAA ATGTGAACCA GTCTTTGTGT CAAGAAGATG ACTATGAAGA CGATAAACCA
    ACCAACTATA GCGAACGTTA CTCTGAGGAA GGGCAGCATG AGGAAGAAGA GAGGCCAACC
    AATTATAGCA TAAAATACAG TGAAGAAAAA CATCACGTGG ATCAGCCTAT TGATTATAGT
    TTAAAATACA CCACAGACAT TCCTTCTTCA CAGAAACCAG CATTTTCATT CTCAAAGAAT
    TCATCTGGAC AGAGCACAAA AACTGAACAC ATCTCTTCAA GCAGCGAGAA TACATCCACA
    ACTTCATCTA ATGCCAAGAG GCAGAATCAG CTGCATCCAA GCTCAGCACA GAGCAGAAGT
    GGTCAGACCC CAAAAGCCAC CTCTTCCTCT TGCAAAGTCC CCTCTATCAA CCAAGAAACA
    ATACAGACTT ACTGTGTAGA AGATACCCCA ATATGTTTTT CAAGATGCAG TTCATTATCA
    TCTCTGTCAT CTGCTGAAGA TGAAGTAGGG TGTGATCAGA CAACACAAGA AGCAGAGTCT
    GCTAACACTC TGCAAATAGC TGAAATCAAG GACAACAGCG GACCTAGGTC AAATGAAGAT
    TCTGTGAGTA AAGTTCCAGC AGGGTCACAG CACATTAGAA CCAAATCCAG CAGACTCCAG
    GCTTCTGGTC TGTCTTCAGA GTCAGCCAGG CACAAAGCTG TTGAATTTTC TTCAGGGGCC
    AAATCTCCTT CCAAGAGCGG TGCTCAGACA CCCAAAAGTC CACCAGAGCA CTACGTTCAG
    GAGACCCCAC TCATGTTTAG CAGATGTACT TCAGTCAGTT CACTCGACAG TTTTGAGAGT
    CGCTCGATTG CCAGCTCTGT TCAGAGTGAA CCCTGCAGTG GAATGGTAAG TGGCATCATA
    AGCCCCAGTG ACCTCCCAGA TAGCCCTGGA CAAACCATGC CACCAAGCAG AAGCAAAACC
    CCTCCGCCGC CGCCTCCGCC TCCTCCTCAG ACAGTTCAAA CGAAGCAGGA AGTACCTAAA
    AATAAAGCAC CTAGTGCTGA AAAGAGAGAA AGTGGACCCA AGCAAGCTGC TGTAAATGCT
    GCAGTACAAA GGGTCCAGGT TCTTCCAGAG GCTGATACTC TGTTACATTT TGCCACAGAG
    AGTACTCCTG ATGGATTTTC TTGTTCATCT AGCCTGAGCG CTCTGAGCCT TGATGAGCCA
    TTTATTCAGA AAGATGTGGA ACTAAGAATA ATGCCTCCAG TTCAGGAAAA TGACAACGGG
    AATGAAACAG AAAGTGAGCA GCCTGAAGAA TCAAATGAAA ACCAGGAAAA AGAGGCAGAA
    AAACCCACTG ATTCTGAAAA AGATCTTTTA GATGAGTCAG ATGATGATGA TATTGAAATA
    CTAGAAGAGT GTATAATTTC TGCCATGCCA ACAAAATCTT CACGCAAAGC CAAAAAACCA
    GCCCAGACTA CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTCCCTGTA
    TACAAACTTC TGCCATCGCA AAACAGGTTA CAGGCACAAA AGCATGTTAG TTTTACACCA
    GGAGATGATA TGCCACGGGT GTATTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCT
    ACATCTCTGA GTGATCTAAC GATAGAATCC CCTCCAAATG AGTTAGCTGC TGGAGAAGGG
    GTTAGAGCAG GGGCACAGTC AAGTGAGTTT GAAAAACGAG ATACCATTCC TACTGAAGGC
    AGAAGTACAG ATGAGGCTCA ACGAGGGAAA GCCTCATCTG TCACTGTGCC TGAACTGGAC
    GACAGCAAAA CAGAAGAAGG TGATATTCTT GCAGAATGCA TTAATTCTGC CATGCCCAAA
    GGAAAAAGTC ACAAGCCTTT CCGTGTGAAA AAGATCATGG ACCAGGTCCA GCAAGCATCT
    ATGTCTTCAT CTGGAACTAA CAAAAATCAA TTAGATGGTA AGACGAAGAA ACCTACTTCA
    CCAGTAAAAC CTATACCACA AAATACTGAA TACAGGACAC GTGTAAGAAA AAATACAGAC
    TCAAAAAATA ATCTAAATGC AGAAAGAAAT TTCTCGGAAA ACAAAGACTC AAAGAAACAG
    CACTTGAAAA ATAATTCCAA GGACTTCAAT GATAAACTGC CAAATAATGA AGACAGAGTC
    AGAGGAAGTT TTACTTTTGA TTCACCCCAT CATTACACGC CCATTGAAGG CACTCCGTAC
    TGTTTTTCAC GAAATGACTC TCTGAGTTCT CTAGATTTTG ATGATGATGA TGTTGACCTT
    TCCAGGGAAA AGGCTGAATT AAGAAAGGGG AAGGAAAATA AAGAATCAGA AGCTAAAGTG
    ACCAACCACA CAGAACTAAC CTCAAACCAA CAATCAGCTA GTAAGACACC AGCTGTTACA
    AAGCAGCCAA TAAATAGAGG TCAGTCTAAA CCCGTGCTGC AGAAGCAGTC CACTTTTCCC
    CAGTCTTCCA AAGATATACC AGACAGAGGG GCAGCAACAG ATGAGAAATT ACAGAATTTC
    GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTAAGCTC TCTTAGTGAC
    ATTGATCAAG AAAACAACAA CAACAAGGAA AATGAACCTG TCAAAGAGAC AGAGCCCCCT
    GCCTCACAGG GAGAACCAGG TAAACCCCAG GCCTCAGGTT ATGCTCCTAA ATCGTTTCAC
    GTGGAAGACA CCCCTGTTTG TTTCTCAAGA AACAGTTCTC TCAGTTCTCT TAGTATTGAT
    TCTGAAGATG ACCTGCTGCA GGAATGTATA AGTTCTGCAA TGCCAAAAAA GAAAAAGCCT
    TCAAGGCTCA AGCCCGATAA TGAAAAGCAT AGTCCCAGAA ATATGGGTGG CATATTAGCA
    GAAGATTTGA CACTCGATTT GAAAGATATA CAGAGACCAG ATTCAGAACA TGGTTTATCC
    CCAGATTCAG AAAATTTTGA TTGGAAAGCT ATTCAGGAAG GTGCAAATTC CATAGTAAGT
    AGTTTACATC AAGCTGCTGC CGCTGCATGT TTATCTCGAC AAGCTTCATC TGATTCAGAT
    TCTATCCTTT CATTGAAATC GGGTATCTCT CTGGGATCAC CATTTCACCT TACACCTGAT
    CAAGAAGAAA AACCCTTTAC GAGTAATAAA GGTCCACGAA TTCTAAAACC TGGGGAGAAA
    AGTACATTGG AAACTAAAAA AATAGAATCT GAAAATAAAG GAATAAAAGG AGGCAAAAAG
    GTTTATAAAA GTTTGATTAC TGGAAAAGTT CGATCTAATT CGGAAATTTC GAGCCAAATG
    AAACAACCCC TTCAAACAAA CATGCCTTCA ATCTCTCGAG GTAGGACAAT GATTCATATT
    CCAGGAGTTC GGAATAGCTC TTCAAGTACA AGCCCAGTTT CTAAAAAAGG CCCGCCCCTC
    AAGACTCCAG CCTCCAAAAG CCCTAGTGAA GGTCAGCCGG CTACCACCTC TCCCAGAGGA
    ACCAAGCCAT CAGTGAAGTC AGAATTAAGC CCTGTTACAA GGCAGGCATC CCAGACAGCA
    GGATCAAACA AAGGACCTTC TAGATCAGGA TCTAGAGATT CCACTCCTTC AAGACCTGCC
    CAGCAACCAT TAAGTAGACC AATGCAGTCT CCAGGGCGAA ACTCAATCTC TCCTGGTAGA
    AATGGAATAA GTCCCCCTAA CAAATTATCT CAACTACCAA GGACGTCATC CCCTAGTACT
    GCTTCAACTA AGTCCTCAGG TTCTGGGAAA ATGTCTTACA CATCTCCTGG CAGACAGATG
    AGCCAGCAGA ACCTCACCAA ACAAACAGGC TTATCCAAGA ATGGCAGTGG TATCCCAAGA
    AGTGAATCTG CCTCCAAAGG GCTAAATCAA ATGAGTAATA GTAATGGATC CAATAAAAAA
    GTAGAACTTT CTAGAATGTC TTCAACAAAG TCAAGTGGAA GTGAATCCGA TAGGTCAGAG
    AGACCTGTAT TAGTACGCCA ATCAACTTTC ATCAAAGAAG CTCCAAGCCC AACCCTAAGG
    AGAAAACTGG AGGAATCTGC TTCATTTGAA TCTCTTTCTC CATCTTCCAG ACCCGATTCT
    CCCACACGGT CCCAGGCTCA TACTCCAGTT TTAAGTCCTT CCCTTCCTGA TATGTCTCTA
    TCTACACATT CGTCTCTTCA GTCTGGTGGA TGGCGAAAAC TCCCACCTAA CCTCAGTCCC
    ACCATAGAGT ATAATGATGG AAGACCAGTA AAGCGCCATG ATATAGCACG CTCTCATTCC
    GAAAGTCCTT CCAGACTTCC CATCAATAGG TCAGGGACCT GGAAACGTGA GCACAGCAAA
    CACTCATCAT CACTTCCTCG AGTAAGCACT TGGAGAAGAA CTGGAAGTTC ATCCTCAATT
    CTTTCTGCTT CATCAGAATC TAGTGAAAAG GCAAAAAGTG AGGACGAAAA ACAAGTGAAC
    TCTATTTCAG GAAGCAAACA AACTAAAGAA AACCAGGTAT CCACAAAAGG AACATGGAGA
    AAAATAAAAG AAAGTGAAAT TTCTCCCACA AATAGTACTT CTCAGACCAC TTCTTCAGGT
    GCTGCAAATG GTGCTGAATC AAAGACTCTG ATTTATCAAA TGGCACCTGC TGTTTCTAAA
    ACAGAGGATG TTTGGGTGAG AATTGAGGAT TGCCCCATTA ACAACCCTAG ATCTGGAAGA
    TCTCCAACAG GAAATACTCC CCCCGTGATT GACACTGTTT CAGAAAAGGG AAACCCGAAC
    CCTAAAGATT CAAAAGATAA TCAGGGAAAA CAAAATGTGA GCAATGGTAG TGCCCCTACA
    CGCACCATGG GTCTGGAAAA CCGCCTGAAT TCCTTTATTC AGGTAGATCC TCCAGACCAA
    AAAGGAACTG AGACAAAACC GGGACACAGT AATAACCCTG TCCCTGCATC CGAGACTAGT
    GAAAGTTCTA TAGCCGAGCG TACCCCGTTT AGTTCTAGCA GCTCAAGCAA GCACAGTTCA
    CCAAGTGGGA CTGTCGCCGC CAGGGTGAGT CCTTTTAATT ACAACCCAAG CCCAAGGAAG
    AGCAGCACAG ATGGCACTTC AGCCCGACCG TCTCAGATCC CAACGCCAGT GAGCAACAAC
    ACAAAGAAAC GCGACTCAAA ACCCGACAGC ACAGAACCCA GCGGGACTCA AAGTCCTAAA
    CGCCATTCTG GGTCTTACCT TGTGACATCT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91580
    Clone ID Related Accession (Same CDS sequence) XM_015472987.1
    Accession Version XM_015472987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTGCCATG TTCTTCTAGA TGATCCTGAG TTAGCTAGAG GACTAAGAAG ACATGAAATT 
    TATGTCAGAT CATTGCTTCT TGCTGACCTT GACAAAGAAG AAAAGGAAAA AGACTGGTAT
    TATGCTCAAC TTCAGAATCT CACTAAAAGA ATAGATAGCC TTCCTTTAAC TGAAAATTTT
    TCCTTACAAA CAGATATGAC CAGAAGGCAG TTGGAATATG AGGCAAGGCA AATCAGAGTT
    GCAATGGAAG AACAACTAGG TACTTGCCAG GATATGGAAA AACGAGCACA GCGAAGAATA
    ACCAGAATTC AACAAATAGA AAAGGACATA CTTCGTATAC GACAGCTTTT ACAGTCCCAA
    GCAACAGAAG CAGAGAGGTC ATCTCAGAGC AAGCATGAAG CGGGCTCACA TGAAGCTGAA
    CGGCAGAATG AAGGTCAAGG AGTGGCAGAA ATCAACATGG CAACCTCGGG TAGCGGTCAG
    GTTACTGCTT CTCTGGTCCC TCTGAAGGAT GTCGGTGCAG ACTTAATCAT ACTAAAAAGG
    CCCAGGAAAG ATTCTCAAGA CTCGGGTTCT GGCTTACAGG GTTCAACTAC ACGAATAGAT
    CATGAAACAG CCAGTGTTTT GAGTTCTAGT AGCACACATT CTGCTCCTCG AAGGCTGACG
    AGTCATCTGG GAACCAAGGT GGAAATGGTG TATTCATTGT TGTCAATGCT TGGTACTCAT
    GATAAGGATG ATATGTCGCG AACTTTGCTA GCTATGTCTA GCTCCCAAGA CAGCTGTATA
    TCCATGCGAC AGTCTGGATG TCTTCCTCTC CTCATCCAGC TTTTACATGG CAATGACAAA
    GACTCTGTGT TGTTGGGAAA TTCCCGGGGC AGTAAAGAGG CTCGGGCCAG GGCCAGTGCA
    GCACTCCACA ACATCATTCA CTCACAGCCT GATGACAAGA GAGGCAGGCG TGAAATCCGA
    GTCCTTCATC TTTTGGAACA GATACGAGCT TACTGTGAAA CCTGTTGGGA GTGGCAGGAA
    GCCCATGAAC AAGGGATGGA CCAGGACAAA AATCCAATGC CAGCTCCTGT TGAACATCAG
    ATCTGCCCTG CTGTCTGTGT TCTAATGAAG CTTTCGTTTG ATGAAGAGCA TAGACATGCC
    ATGAATGAGC TTGGTAGGAA GGCTACCCGG GGCATTTCAT CCCAGGAGCT AGGGCAGGGG
    CTTTCAGGGG GACTACAGGC CATTGCAGAA TTATTGCAAG TGGACTGTGA AATGTATGGG
    CTTACTAATG ACCACTACAG TATTACTTTA AGACGATATG CAGGAATGGC TTTGACAAAC
    TTGACTTTCG GAGATGTAGC CAACAAGGCT ACTCTCTGCT CTATGAAAGG CTGCATGAGA
    GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC GAGGACTTGC AGCAGGTTAT TGCGAGTGTT
    TTGAGGAATC TGTCTTGGAG AGCAGATGTA AATAGTAAAA AGACTTTGCG TGAAGTTGGA
    AGTGTGAAAG CATTGATGGA ATGTGCTTTG GAAGTGAAAA AGGAATCAAC CCTCAAAAGT
    GTATTGAGTG CCTTATGGAA CTTGTCAGCA CACTGCACTG AGAATAAAGC TGACATATGT
    GCTGTGGATG GTGCGCTTGC ATTTTTGGTT GGCACTCTCA CTTATCGCAG CCAGACCAAT
    ACTTTAGCCA TTATTGAAAG TGGAGGTGGT ATATTACGGA ATGTATCCAG CTTGATAGCT
    ACAAATGAGG ACCACAGGCA AATCCTAAGA GAGAATAATT GCTTACAGAC CTTATTACAA
    CACTTGAAAT CTCACAGTTT GACAATAGTC AGCAATGCAT GCGGAACCTT GTGGAATCTC
    TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA TTGTGGGACA TGGGAGCAGT CAGCATGCTC
    AAGAACCTCA TTCATTCAAA GCACAAGATG ATTGCTATGG GAAGTGCTGC AGCTTTAAGG
    AATCTCATGG CAAATAGACC TGCAAAGTAT AAAGATGCCA ATATCATGTC TCCTGGTTCA
    AGTTTGCCTT CTCTACATGT CAGGAAACAA AAGGCCCTGG AAGCAGAATT AGATGCTCAG
    CATTTATCAG AAACTTTTGA CAATATTGAC AATTTAAGTC CCAAGGCATC TCATCGTAGC
    AAGCAGAGAC ACAAGCAAAA TCTCTATGGT GACTATGTTT TTGACACCAA TCGACATGAT
    GATAACAGGT CAGACAATTT TAATACTGGA AACATGACTG TCCTATCACC GTACTTAAAC
    ACTACAGTAT TGCCCAGCTC TTCTTCATCA AGGGGAAGTT TAGATAGCTC TCGTTCTGAG
    AAAGATAGAA GTCTGGAGAG AGAACGAGGT ATTAGCCTAG GCAACTATCA CCCAGCAACA
    GAAAACCCAG GAACCTCTTC AAAGCGAGGT TTGCAGATTT CTACCACTGC AGCCCAGATT
    GCCAAAGTCA TGGAAGAAGT ATCAGCTATT CATACCTCTC AGGAAGACAG AAGTTCTGGG
    TCTACCACAG AACTACACTG TGGGACAGAT GAGAGGAATG CACTAAGAAG AAGCTCTACC
    ACCCACACAC ATGCAAACAC GTACAACTTT ACCAAGTCAG AAAACTCAAA CAGAACATGT
    CCAATACCAT ATGCCAAAGT AGAATATAAG AGATCTTCAA ATGATAGTTT AAATAGTGTC
    AGCAGTAGTG ATGGTTATGG TAAAAGAGGT CAGATGAAAC CTTCAATTGA ATCCTATTCT
    GAAGATGATG AAAGTAAATT TTGCAGCTAT GGTCAGTATC CAGCTGACCT AGCCCATAAA
    ATACATAGTG CAAATCATAT GGATGATAAT GATGGAGAAC TAGATACACC AATAAATTAC
    AGTCTCAAAT ATTCCGATGA ACAGTTGAAC TCTGGGAGGC AGAGCCCTTC ACAGAATGAA
    AGATGGGCAA GACCCAAACA TATACTAGAA GATGAAATAA AACCGAATGA GCAGAGACAA
    TCAAGGAGTC AAAGCACAGC TTATCCCGTG TATCCTGAGA GCACTGATGA TAAACACCTC
    AAGTTCCAAC CACACTTTGG GCAGCAAGAA TGTGTTTCCC CATACAGGTC AAGAGCAGCC
    AATGGATCTG AAACAAATCG AGTAGGCTCT AATCATGGAA TTAGTCAAAA TGTGAACCAG
    TCTTTGTGTC AAGAAGATGA CTATGAAGAC GATAAACCAA CCAACTATAG CGAACGTTAC
    TCTGAGGAAG GGCAGCATGA GGAAGAAGAG AGGCCAACCA ATTATAGCAT AAAATACAGT
    GAAGAAAAAC ATCACGTGGA TCAGCCTATT GATTATAGTT TAAAATACAC CACAGACATT
    CCTTCTTCAC AGAAACCAGC ATTTTCATTC TCAAAGAATT CATCTGGACA GAGCACAAAA
    ACTGAACACA TCTCTTCAAG CAGCGAGAAT ACATCCACAA CTTCATCTAA TGCCAAGAGG
    CAGAATCAGC TGCATCCAAG CTCAGCACAG AGCAGAAGTG GTCAGACCCC AAAAGCCACC
    TCTTCCTCTT GCAAAGTCCC CTCTATCAAC CAAGAAACAA TACAGACTTA CTGTGTAGAA
    GATACCCCAA TATGTTTTTC AAGATGCAGT TCATTATCAT CTCTGTCATC TGCTGAAGAT
    GAAGTAGGGT GTGATCAGAC AACACAAGAA GCAGAGTCTG CTAACACTCT GCAAATAGCT
    GAAATCAAGG ACAACAGCGG ACCTAGGTCA AATGAAGATT CTGTGAGTAA AGTTCCAGCA
    GGGTCACAGC ACATTAGAAC CAAATCCAGC AGACTCCAGG CTTCTGGTCT GTCTTCAGAG
    TCAGCCAGGC ACAAAGCTGT TGAATTTTCT TCAGGGGCCA AATCTCCTTC CAAGAGCGGT
    GCTCAGACAC CCAAAAGTCC ACCAGAGCAC TACGTTCAGG AGACCCCACT CATGTTTAGC
    AGATGTACTT CAGTCAGTTC ACTCGACAGT TTTGAGAGTC GCTCGATTGC CAGCTCTGTT
    CAGAGTGAAC CCTGCAGTGG AATGGTAAGT GGCATCATAA GCCCCAGTGA CCTCCCAGAT
    AGCCCTGGAC AAACCATGCC ACCAAGCAGA AGCAAAACCC CTCCGCCGCC GCCTCCGCCT
    CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA GTACCTAAAA ATAAAGCACC TAGTGCTGAA
    AAGAGAGAAA GTGGACCCAA GCAAGCTGCT GTAAATGCTG CAGTACAAAG GGTCCAGGTT
    CTTCCAGAGG CTGATACTCT GTTACATTTT GCCACAGAGA GTACTCCTGA TGGATTTTCT
    TGTTCATCTA GCCTGAGCGC TCTGAGCCTT GATGAGCCAT TTATTCAGAA AGATGTGGAA
    CTAAGAATAA TGCCTCCAGT TCAGGAAAAT GACAACGGGA ATGAAACAGA AAGTGAGCAG
    CCTGAAGAAT CAAATGAAAA CCAGGAAAAA GAGGCAGAAA AACCCACTGA TTCTGAAAAA
    GATCTTTTAG ATGAGTCAGA TGATGATGAT ATTGAAATAC TAGAAGAGTG TATAATTTCT
    GCCATGCCAA CAAAATCTTC ACGCAAAGCC AAAAAACCAG CCCAGACTAC TTCAAAATTA
    CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTCCCTGTAT ACAAACTTCT GCCATCGCAA
    AACAGGTTAC AGGCACAAAA GCATGTTAGT TTTACACCAG GAGATGATAT GCCACGGGTG
    TATTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCTA CATCTCTGAG TGATCTAACG
    ATAGAATCCC CTCCAAATGA GTTAGCTGCT GGAGAAGGGG TTAGAGCAGG GGCACAGTCA
    AGTGAGTTTG AAAAACGAGA TACCATTCCT ACTGAAGGCA GAAGTACAGA TGAGGCTCAA
    CGAGGGAAAG CCTCATCTGT CACTGTGCCT GAACTGGACG ACAGCAAAAC AGAAGAAGGT
    GATATTCTTG CAGAATGCAT TAATTCTGCC ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC
    CGTGTGAAAA AGATCATGGA CCAGGTCCAG CAAGCATCTA TGTCTTCATC TGGAACTAAC
    AAAAATCAAT TAGATGGTAA GACGAAGAAA CCTACTTCAC CAGTAAAACC TATACCACAA
    AATACTGAAT ACAGGACACG TGTAAGAAAA AATACAGACT CAAAAAATAA TCTAAATGCA
    GAAAGAAATT TCTCGGAAAA CAAAGACTCA AAGAAACAGC ACTTGAAAAA TAATTCCAAG
    GACTTCAATG ATAAACTGCC AAATAATGAA GACAGAGTCA GAGGAAGTTT TACTTTTGAT
    TCACCCCATC ATTACACGCC CATTGAAGGC ACTCCGTACT GTTTTTCACG AAATGACTCT
    CTGAGTTCTC TAGATTTTGA TGATGATGAT GTTGACCTTT CCAGGGAAAA GGCTGAATTA
    AGAAAGGGGA AGGAAAATAA AGAATCAGAA GCTAAAGTGA CCAACCACAC AGAACTAACC
    TCAAACCAAC AATCAGCTAG TAAGACACCA GCTGTTACAA AGCAGCCAAT AAATAGAGGT
    CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC ACTTTTCCCC AGTCTTCCAA AGATATACCA
    GACAGAGGGG CAGCAACAGA TGAGAAATTA CAGAATTTCG CTATTGAAAA TACTCCAGTT
    TGCTTTTCTC GAAATTCCTC TCTAAGCTCT CTTAGTGACA TTGATCAAGA AAACAACAAC
    AACAAGGAAA ATGAACCTGT CAAAGAGACA GAGCCCCCTG CCTCACAGGG AGAACCAGGT
    AAACCCCAGG CCTCAGGTTA TGCTCCTAAA TCGTTTCACG TGGAAGACAC CCCTGTTTGT
    TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT AGTATTGATT CTGAAGATGA CCTGCTGCAG
    GAATGTATAA GTTCTGCAAT GCCAAAAAAG AAAAAGCCTT CAAGGCTCAA GCCCGATAAT
    GAAAAGCATA GTCCCAGAAA TATGGGTGGC ATATTAGCAG AAGATTTGAC ACTCGATTTG
    AAAGATATAC AGAGACCAGA TTCAGAACAT GGTTTATCCC CAGATTCAGA AAATTTTGAT
    TGGAAAGCTA TTCAGGAAGG TGCAAATTCC ATAGTAAGTA GTTTACATCA AGCTGCTGCC
    GCTGCATGTT TATCTCGACA AGCTTCATCT GATTCAGATT CTATCCTTTC ATTGAAATCG
    GGTATCTCTC TGGGATCACC ATTTCACCTT ACACCTGATC AAGAAGAAAA ACCCTTTACG
    AGTAATAAAG GTCCACGAAT TCTAAAACCT GGGGAGAAAA GTACATTGGA AACTAAAAAA
    ATAGAATCTG AAAATAAAGG AATAAAAGGA GGCAAAAAGG TTTATAAAAG TTTGATTACT
    GGAAAAGTTC GATCTAATTC GGAAATTTCG AGCCAAATGA AACAACCCCT TCAAACAAAC
    ATGCCTTCAA TCTCTCGAGG TAGGACAATG ATTCATATTC CAGGAGTTCG GAATAGCTCT
    TCAAGTACAA GCCCAGTTTC TAAAAAAGGC CCGCCCCTCA AGACTCCAGC CTCCAAAAGC
    CCTAGTGAAG GTCAGCCGGC TACCACCTCT CCCAGAGGAA CCAAGCCATC AGTGAAGTCA
    GAATTAAGCC CTGTTACAAG GCAGGCATCC CAGACAGCAG GATCAAACAA AGGACCTTCT
    AGATCAGGAT CTAGAGATTC CACTCCTTCA AGACCTGCCC AGCAACCATT AAGTAGACCA
    ATGCAGTCTC CAGGGCGAAA CTCAATCTCT CCTGGTAGAA ATGGAATAAG TCCCCCTAAC
    AAATTATCTC AACTACCAAG GACGTCATCC CCTAGTACTG CTTCAACTAA GTCCTCAGGT
    TCTGGGAAAA TGTCTTACAC ATCTCCTGGC AGACAGATGA GCCAGCAGAA CCTCACCAAA
    CAAACAGGCT TATCCAAGAA TGGCAGTGGT ATCCCAAGAA GTGAATCTGC CTCCAAAGGG
    CTAAATCAAA TGAGTAATAG TAATGGATCC AATAAAAAAG TAGAACTTTC TAGAATGTCT
    TCAACAAAGT CAAGTGGAAG TGAATCCGAT AGGTCAGAGA GACCTGTATT AGTACGCCAA
    TCAACTTTCA TCAAAGAAGC TCCAAGCCCA ACCCTAAGGA GAAAACTGGA GGAATCTGCT
    TCATTTGAAT CTCTTTCTCC ATCTTCCAGA CCCGATTCTC CCACACGGTC CCAGGCTCAT
    ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT ATGTCTCTAT CTACACATTC GTCTCTTCAG
    TCTGGTGGAT GGCGAAAACT CCCACCTAAC CTCAGTCCCA CCATAGAGTA TAATGATGGA
    AGACCAGTAA AGCGCCATGA TATAGCACGC TCTCATTCCG AAAGTCCTTC CAGACTTCCC
    ATCAATAGGT CAGGGACCTG GAAACGTGAG CACAGCAAAC ACTCATCATC ACTTCCTCGA
    GTAAGCACTT GGAGAAGAAC TGGAAGTTCA TCCTCAATTC TTTCTGCTTC ATCAGAATCT
    AGTGAAAAGG CAAAAAGTGA GGACGAAAAA CAAGTGAACT CTATTTCAGG AAGCAAACAA
    ACTAAAGAAA ACCAGGTATC CACAAAAGGA ACATGGAGAA AAATAAAAGA AAGTGAAATT
    TCTCCCACAA ATAGTACTTC TCAGACCACT TCTTCAGGTG CTGCAAATGG TGCTGAATCA
    AAGACTCTGA TTTATCAAAT GGCACCTGCT GTTTCTAAAA CAGAGGATGT TTGGGTGAGA
    ATTGAGGATT GCCCCATTAA CAACCCTAGA TCTGGAAGAT CTCCAACAGG AAATACTCCC
    CCCGTGATTG ACACTGTTTC AGAAAAGGGA AACCCGAACC CTAAAGATTC AAAAGATAAT
    CAGGGAAAAC AAAATGTGAG CAATGGTAGT GCCCCTACAC GCACCATGGG TCTGGAAAAC
    CGCCTGAATT CCTTTATTCA GGTAGATCCT CCAGACCAAA AAGGAACTGA GACAAAACCG
    GGACACAGTA ATAACCCTGT CCCTGCATCC GAGACTAGTG AAAGTTCTAT AGCCGAGCGT
    ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG CACAGTTCAC CAAGTGGGAC TGTCGCCGCC
    AGGGTGAGTC CTTTTAATTA CAACCCAAGC CCAAGGAAGA GCAGCACAGA TGGCACTTCA
    GCCCGACCGT CTCAGATCCC AACGCCAGTG AGCAACAACA CAAAGAAACG CGACTCAAAA
    CCCGACAGCA CAGAACCCAG CGGGACTCAA AGTCCTAAAC GCCATTCTGG GTCTTACCTT
    GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328473.1
    CDS16..8370
    Translation

    Target ORF information:

    RefSeq Version XM_015472987.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGTGCCATG TTCTTCTAGA TGATCCTGAG TTAGCTAGAG GACTAAGAAG ACATGAAATT 
    TATGTCAGAT CATTGCTTCT TGCTGACCTT GACAAAGAAG AAAAGGAAAA AGACTGGTAT
    TATGCTCAAC TTCAGAATCT CACTAAAAGA ATAGATAGCC TTCCTTTAAC TGAAAATTTT
    TCCTTACAAA CAGATATGAC CAGAAGGCAG TTGGAATATG AGGCAAGGCA AATCAGAGTT
    GCAATGGAAG AACAACTAGG TACTTGCCAG GATATGGAAA AACGAGCACA GCGAAGAATA
    ACCAGAATTC AACAAATAGA AAAGGACATA CTTCGTATAC GACAGCTTTT ACAGTCCCAA
    GCAACAGAAG CAGAGAGGTC ATCTCAGAGC AAGCATGAAG CGGGCTCACA TGAAGCTGAA
    CGGCAGAATG AAGGTCAAGG AGTGGCAGAA ATCAACATGG CAACCTCGGG TAGCGGTCAG
    GTTACTGCTT CTCTGGTCCC TCTGAAGGAT GTCGGTGCAG ACTTAATCAT ACTAAAAAGG
    CCCAGGAAAG ATTCTCAAGA CTCGGGTTCT GGCTTACAGG GTTCAACTAC ACGAATAGAT
    CATGAAACAG CCAGTGTTTT GAGTTCTAGT AGCACACATT CTGCTCCTCG AAGGCTGACG
    AGTCATCTGG GAACCAAGGT GGAAATGGTG TATTCATTGT TGTCAATGCT TGGTACTCAT
    GATAAGGATG ATATGTCGCG AACTTTGCTA GCTATGTCTA GCTCCCAAGA CAGCTGTATA
    TCCATGCGAC AGTCTGGATG TCTTCCTCTC CTCATCCAGC TTTTACATGG CAATGACAAA
    GACTCTGTGT TGTTGGGAAA TTCCCGGGGC AGTAAAGAGG CTCGGGCCAG GGCCAGTGCA
    GCACTCCACA ACATCATTCA CTCACAGCCT GATGACAAGA GAGGCAGGCG TGAAATCCGA
    GTCCTTCATC TTTTGGAACA GATACGAGCT TACTGTGAAA CCTGTTGGGA GTGGCAGGAA
    GCCCATGAAC AAGGGATGGA CCAGGACAAA AATCCAATGC CAGCTCCTGT TGAACATCAG
    ATCTGCCCTG CTGTCTGTGT TCTAATGAAG CTTTCGTTTG ATGAAGAGCA TAGACATGCC
    ATGAATGAGC TTGGTAGGAA GGCTACCCGG GGCATTTCAT CCCAGGAGCT AGGGCAGGGG
    CTTTCAGGGG GACTACAGGC CATTGCAGAA TTATTGCAAG TGGACTGTGA AATGTATGGG
    CTTACTAATG ACCACTACAG TATTACTTTA AGACGATATG CAGGAATGGC TTTGACAAAC
    TTGACTTTCG GAGATGTAGC CAACAAGGCT ACTCTCTGCT CTATGAAAGG CTGCATGAGA
    GCGCTCGTGG CTCAGCTCCA GTCTGAAAGC GAGGACTTGC AGCAGGTTAT TGCGAGTGTT
    TTGAGGAATC TGTCTTGGAG AGCAGATGTA AATAGTAAAA AGACTTTGCG TGAAGTTGGA
    AGTGTGAAAG CATTGATGGA ATGTGCTTTG GAAGTGAAAA AGGAATCAAC CCTCAAAAGT
    GTATTGAGTG CCTTATGGAA CTTGTCAGCA CACTGCACTG AGAATAAAGC TGACATATGT
    GCTGTGGATG GTGCGCTTGC ATTTTTGGTT GGCACTCTCA CTTATCGCAG CCAGACCAAT
    ACTTTAGCCA TTATTGAAAG TGGAGGTGGT ATATTACGGA ATGTATCCAG CTTGATAGCT
    ACAAATGAGG ACCACAGGCA AATCCTAAGA GAGAATAATT GCTTACAGAC CTTATTACAA
    CACTTGAAAT CTCACAGTTT GACAATAGTC AGCAATGCAT GCGGAACCTT GTGGAATCTC
    TCAGCAAGAA ACCCTAAAGA CCAGGAAGCA TTGTGGGACA TGGGAGCAGT CAGCATGCTC
    AAGAACCTCA TTCATTCAAA GCACAAGATG ATTGCTATGG GAAGTGCTGC AGCTTTAAGG
    AATCTCATGG CAAATAGACC TGCAAAGTAT AAAGATGCCA ATATCATGTC TCCTGGTTCA
    AGTTTGCCTT CTCTACATGT CAGGAAACAA AAGGCCCTGG AAGCAGAATT AGATGCTCAG
    CATTTATCAG AAACTTTTGA CAATATTGAC AATTTAAGTC CCAAGGCATC TCATCGTAGC
    AAGCAGAGAC ACAAGCAAAA TCTCTATGGT GACTATGTTT TTGACACCAA TCGACATGAT
    GATAACAGGT CAGACAATTT TAATACTGGA AACATGACTG TCCTATCACC GTACTTAAAC
    ACTACAGTAT TGCCCAGCTC TTCTTCATCA AGGGGAAGTT TAGATAGCTC TCGTTCTGAG
    AAAGATAGAA GTCTGGAGAG AGAACGAGGT ATTAGCCTAG GCAACTATCA CCCAGCAACA
    GAAAACCCAG GAACCTCTTC AAAGCGAGGT TTGCAGATTT CTACCACTGC AGCCCAGATT
    GCCAAAGTCA TGGAAGAAGT ATCAGCTATT CATACCTCTC AGGAAGACAG AAGTTCTGGG
    TCTACCACAG AACTACACTG TGGGACAGAT GAGAGGAATG CACTAAGAAG AAGCTCTACC
    ACCCACACAC ATGCAAACAC GTACAACTTT ACCAAGTCAG AAAACTCAAA CAGAACATGT
    CCAATACCAT ATGCCAAAGT AGAATATAAG AGATCTTCAA ATGATAGTTT AAATAGTGTC
    AGCAGTAGTG ATGGTTATGG TAAAAGAGGT CAGATGAAAC CTTCAATTGA ATCCTATTCT
    GAAGATGATG AAAGTAAATT TTGCAGCTAT GGTCAGTATC CAGCTGACCT AGCCCATAAA
    ATACATAGTG CAAATCATAT GGATGATAAT GATGGAGAAC TAGATACACC AATAAATTAC
    AGTCTCAAAT ATTCCGATGA ACAGTTGAAC TCTGGGAGGC AGAGCCCTTC ACAGAATGAA
    AGATGGGCAA GACCCAAACA TATACTAGAA GATGAAATAA AACCGAATGA GCAGAGACAA
    TCAAGGAGTC AAAGCACAGC TTATCCCGTG TATCCTGAGA GCACTGATGA TAAACACCTC
    AAGTTCCAAC CACACTTTGG GCAGCAAGAA TGTGTTTCCC CATACAGGTC AAGAGCAGCC
    AATGGATCTG AAACAAATCG AGTAGGCTCT AATCATGGAA TTAGTCAAAA TGTGAACCAG
    TCTTTGTGTC AAGAAGATGA CTATGAAGAC GATAAACCAA CCAACTATAG CGAACGTTAC
    TCTGAGGAAG GGCAGCATGA GGAAGAAGAG AGGCCAACCA ATTATAGCAT AAAATACAGT
    GAAGAAAAAC ATCACGTGGA TCAGCCTATT GATTATAGTT TAAAATACAC CACAGACATT
    CCTTCTTCAC AGAAACCAGC ATTTTCATTC TCAAAGAATT CATCTGGACA GAGCACAAAA
    ACTGAACACA TCTCTTCAAG CAGCGAGAAT ACATCCACAA CTTCATCTAA TGCCAAGAGG
    CAGAATCAGC TGCATCCAAG CTCAGCACAG AGCAGAAGTG GTCAGACCCC AAAAGCCACC
    TCTTCCTCTT GCAAAGTCCC CTCTATCAAC CAAGAAACAA TACAGACTTA CTGTGTAGAA
    GATACCCCAA TATGTTTTTC AAGATGCAGT TCATTATCAT CTCTGTCATC TGCTGAAGAT
    GAAGTAGGGT GTGATCAGAC AACACAAGAA GCAGAGTCTG CTAACACTCT GCAAATAGCT
    GAAATCAAGG ACAACAGCGG ACCTAGGTCA AATGAAGATT CTGTGAGTAA AGTTCCAGCA
    GGGTCACAGC ACATTAGAAC CAAATCCAGC AGACTCCAGG CTTCTGGTCT GTCTTCAGAG
    TCAGCCAGGC ACAAAGCTGT TGAATTTTCT TCAGGGGCCA AATCTCCTTC CAAGAGCGGT
    GCTCAGACAC CCAAAAGTCC ACCAGAGCAC TACGTTCAGG AGACCCCACT CATGTTTAGC
    AGATGTACTT CAGTCAGTTC ACTCGACAGT TTTGAGAGTC GCTCGATTGC CAGCTCTGTT
    CAGAGTGAAC CCTGCAGTGG AATGGTAAGT GGCATCATAA GCCCCAGTGA CCTCCCAGAT
    AGCCCTGGAC AAACCATGCC ACCAAGCAGA AGCAAAACCC CTCCGCCGCC GCCTCCGCCT
    CCTCCTCAGA CAGTTCAAAC GAAGCAGGAA GTACCTAAAA ATAAAGCACC TAGTGCTGAA
    AAGAGAGAAA GTGGACCCAA GCAAGCTGCT GTAAATGCTG CAGTACAAAG GGTCCAGGTT
    CTTCCAGAGG CTGATACTCT GTTACATTTT GCCACAGAGA GTACTCCTGA TGGATTTTCT
    TGTTCATCTA GCCTGAGCGC TCTGAGCCTT GATGAGCCAT TTATTCAGAA AGATGTGGAA
    CTAAGAATAA TGCCTCCAGT TCAGGAAAAT GACAACGGGA ATGAAACAGA AAGTGAGCAG
    CCTGAAGAAT CAAATGAAAA CCAGGAAAAA GAGGCAGAAA AACCCACTGA TTCTGAAAAA
    GATCTTTTAG ATGAGTCAGA TGATGATGAT ATTGAAATAC TAGAAGAGTG TATAATTTCT
    GCCATGCCAA CAAAATCTTC ACGCAAAGCC AAAAAACCAG CCCAGACTAC TTCAAAATTA
    CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTCCCTGTAT ACAAACTTCT GCCATCGCAA
    AACAGGTTAC AGGCACAAAA GCATGTTAGT TTTACACCAG GAGATGATAT GCCACGGGTG
    TATTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCTA CATCTCTGAG TGATCTAACG
    ATAGAATCCC CTCCAAATGA GTTAGCTGCT GGAGAAGGGG TTAGAGCAGG GGCACAGTCA
    AGTGAGTTTG AAAAACGAGA TACCATTCCT ACTGAAGGCA GAAGTACAGA TGAGGCTCAA
    CGAGGGAAAG CCTCATCTGT CACTGTGCCT GAACTGGACG ACAGCAAAAC AGAAGAAGGT
    GATATTCTTG CAGAATGCAT TAATTCTGCC ATGCCCAAAG GAAAAAGTCA CAAGCCTTTC
    CGTGTGAAAA AGATCATGGA CCAGGTCCAG CAAGCATCTA TGTCTTCATC TGGAACTAAC
    AAAAATCAAT TAGATGGTAA GACGAAGAAA CCTACTTCAC CAGTAAAACC TATACCACAA
    AATACTGAAT ACAGGACACG TGTAAGAAAA AATACAGACT CAAAAAATAA TCTAAATGCA
    GAAAGAAATT TCTCGGAAAA CAAAGACTCA AAGAAACAGC ACTTGAAAAA TAATTCCAAG
    GACTTCAATG ATAAACTGCC AAATAATGAA GACAGAGTCA GAGGAAGTTT TACTTTTGAT
    TCACCCCATC ATTACACGCC CATTGAAGGC ACTCCGTACT GTTTTTCACG AAATGACTCT
    CTGAGTTCTC TAGATTTTGA TGATGATGAT GTTGACCTTT CCAGGGAAAA GGCTGAATTA
    AGAAAGGGGA AGGAAAATAA AGAATCAGAA GCTAAAGTGA CCAACCACAC AGAACTAACC
    TCAAACCAAC AATCAGCTAG TAAGACACCA GCTGTTACAA AGCAGCCAAT AAATAGAGGT
    CAGTCTAAAC CCGTGCTGCA GAAGCAGTCC ACTTTTCCCC AGTCTTCCAA AGATATACCA
    GACAGAGGGG CAGCAACAGA TGAGAAATTA CAGAATTTCG CTATTGAAAA TACTCCAGTT
    TGCTTTTCTC GAAATTCCTC TCTAAGCTCT CTTAGTGACA TTGATCAAGA AAACAACAAC
    AACAAGGAAA ATGAACCTGT CAAAGAGACA GAGCCCCCTG CCTCACAGGG AGAACCAGGT
    AAACCCCAGG CCTCAGGTTA TGCTCCTAAA TCGTTTCACG TGGAAGACAC CCCTGTTTGT
    TTCTCAAGAA ACAGTTCTCT CAGTTCTCTT AGTATTGATT CTGAAGATGA CCTGCTGCAG
    GAATGTATAA GTTCTGCAAT GCCAAAAAAG AAAAAGCCTT CAAGGCTCAA GCCCGATAAT
    GAAAAGCATA GTCCCAGAAA TATGGGTGGC ATATTAGCAG AAGATTTGAC ACTCGATTTG
    AAAGATATAC AGAGACCAGA TTCAGAACAT GGTTTATCCC CAGATTCAGA AAATTTTGAT
    TGGAAAGCTA TTCAGGAAGG TGCAAATTCC ATAGTAAGTA GTTTACATCA AGCTGCTGCC
    GCTGCATGTT TATCTCGACA AGCTTCATCT GATTCAGATT CTATCCTTTC ATTGAAATCG
    GGTATCTCTC TGGGATCACC ATTTCACCTT ACACCTGATC AAGAAGAAAA ACCCTTTACG
    AGTAATAAAG GTCCACGAAT TCTAAAACCT GGGGAGAAAA GTACATTGGA AACTAAAAAA
    ATAGAATCTG AAAATAAAGG AATAAAAGGA GGCAAAAAGG TTTATAAAAG TTTGATTACT
    GGAAAAGTTC GATCTAATTC GGAAATTTCG AGCCAAATGA AACAACCCCT TCAAACAAAC
    ATGCCTTCAA TCTCTCGAGG TAGGACAATG ATTCATATTC CAGGAGTTCG GAATAGCTCT
    TCAAGTACAA GCCCAGTTTC TAAAAAAGGC CCGCCCCTCA AGACTCCAGC CTCCAAAAGC
    CCTAGTGAAG GTCAGCCGGC TACCACCTCT CCCAGAGGAA CCAAGCCATC AGTGAAGTCA
    GAATTAAGCC CTGTTACAAG GCAGGCATCC CAGACAGCAG GATCAAACAA AGGACCTTCT
    AGATCAGGAT CTAGAGATTC CACTCCTTCA AGACCTGCCC AGCAACCATT AAGTAGACCA
    ATGCAGTCTC CAGGGCGAAA CTCAATCTCT CCTGGTAGAA ATGGAATAAG TCCCCCTAAC
    AAATTATCTC AACTACCAAG GACGTCATCC CCTAGTACTG CTTCAACTAA GTCCTCAGGT
    TCTGGGAAAA TGTCTTACAC ATCTCCTGGC AGACAGATGA GCCAGCAGAA CCTCACCAAA
    CAAACAGGCT TATCCAAGAA TGGCAGTGGT ATCCCAAGAA GTGAATCTGC CTCCAAAGGG
    CTAAATCAAA TGAGTAATAG TAATGGATCC AATAAAAAAG TAGAACTTTC TAGAATGTCT
    TCAACAAAGT CAAGTGGAAG TGAATCCGAT AGGTCAGAGA GACCTGTATT AGTACGCCAA
    TCAACTTTCA TCAAAGAAGC TCCAAGCCCA ACCCTAAGGA GAAAACTGGA GGAATCTGCT
    TCATTTGAAT CTCTTTCTCC ATCTTCCAGA CCCGATTCTC CCACACGGTC CCAGGCTCAT
    ACTCCAGTTT TAAGTCCTTC CCTTCCTGAT ATGTCTCTAT CTACACATTC GTCTCTTCAG
    TCTGGTGGAT GGCGAAAACT CCCACCTAAC CTCAGTCCCA CCATAGAGTA TAATGATGGA
    AGACCAGTAA AGCGCCATGA TATAGCACGC TCTCATTCCG AAAGTCCTTC CAGACTTCCC
    ATCAATAGGT CAGGGACCTG GAAACGTGAG CACAGCAAAC ACTCATCATC ACTTCCTCGA
    GTAAGCACTT GGAGAAGAAC TGGAAGTTCA TCCTCAATTC TTTCTGCTTC ATCAGAATCT
    AGTGAAAAGG CAAAAAGTGA GGACGAAAAA CAAGTGAACT CTATTTCAGG AAGCAAACAA
    ACTAAAGAAA ACCAGGTATC CACAAAAGGA ACATGGAGAA AAATAAAAGA AAGTGAAATT
    TCTCCCACAA ATAGTACTTC TCAGACCACT TCTTCAGGTG CTGCAAATGG TGCTGAATCA
    AAGACTCTGA TTTATCAAAT GGCACCTGCT GTTTCTAAAA CAGAGGATGT TTGGGTGAGA
    ATTGAGGATT GCCCCATTAA CAACCCTAGA TCTGGAAGAT CTCCAACAGG AAATACTCCC
    CCCGTGATTG ACACTGTTTC AGAAAAGGGA AACCCGAACC CTAAAGATTC AAAAGATAAT
    CAGGGAAAAC AAAATGTGAG CAATGGTAGT GCCCCTACAC GCACCATGGG TCTGGAAAAC
    CGCCTGAATT CCTTTATTCA GGTAGATCCT CCAGACCAAA AAGGAACTGA GACAAAACCG
    GGACACAGTA ATAACCCTGT CCCTGCATCC GAGACTAGTG AAAGTTCTAT AGCCGAGCGT
    ACCCCGTTTA GTTCTAGCAG CTCAAGCAAG CACAGTTCAC CAAGTGGGAC TGTCGCCGCC
    AGGGTGAGTC CTTTTAATTA CAACCCAAGC CCAAGGAAGA GCAGCACAGA TGGCACTTCA
    GCCCGACCGT CTCAGATCCC AACGCCAGTG AGCAACAACA CAAAGAAACG CGACTCAAAA
    CCCGACAGCA CAGAACCCAG CGGGACTCAA AGTCCTAAAC GCCATTCTGG GTCTTACCTT
    GTGACATCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91581
    Clone ID Related Accession (Same CDS sequence) XM_015472988.1
    Accession Version XM_015472988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adenomatous polyposis coli protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGACCAGAA GGCAGTTGGA ATATGAGGCA AGGCAAATCA GAGTTGCAAT GGAAGAACAA 
    CTAGGTACTT GCCAGGATAT GGAAAAACGA GCACAGCGAA GAATAACCAG AATTCAACAA
    ATAGAAAAGG ACATACTTCG TATACGACAG CTTTTACAGT CCCAAGCAAC AGAAGCAGAG
    AGGTCATCTC AGAGCAAGCA TGAAGCGGGC TCACATGAAG CTGAACGGCA GAATGAAGGT
    CAAGGAGTGG CAGAAATCAA CATGGCAACC TCGGGTAGCG GTCAGGTTAC TGCTTCTCTG
    GTCCCTCTGA AGGATGTCGG TGCAGACTTA ATCATACTAA AAAGGCCCAG GAAAGATTCT
    CAAGACTCGG GTTCTGGCTT ACAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCGCCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328474.1
    CDS213..8372
    Translation

    Target ORF information:

    RefSeq Version XM_015472988.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adenomatous polyposis coli (APC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGACCAGAA GGCAGTTGGA ATATGAGGCA AGGCAAATCA GAGTTGCAAT GGAAGAACAA 
    CTAGGTACTT GCCAGGATAT GGAAAAACGA GCACAGCGAA GAATAACCAG AATTCAACAA
    ATAGAAAAGG ACATACTTCG TATACGACAG CTTTTACAGT CCCAAGCAAC AGAAGCAGAG
    AGGTCATCTC AGAGCAAGCA TGAAGCGGGC TCACATGAAG CTGAACGGCA GAATGAAGGT
    CAAGGAGTGG CAGAAATCAA CATGGCAACC TCGGGTAGCG GTCAGGTTAC TGCTTCTCTG
    GTCCCTCTGA AGGATGTCGG TGCAGACTTA ATCATACTAA AAAGGCCCAG GAAAGATTCT
    CAAGACTCGG GTTCTGGCTT ACAGGGTTCA ACTACACGAA TAGATCATGA AACAGCCAGT
    GTTTTGAGTT CTAGTAGCAC ACATTCTGCT CCTCGAAGGC TGACGAGTCA TCTGGGAACC
    AAGGTGGAAA TGGTGTATTC ATTGTTGTCA ATGCTTGGTA CTCATGATAA GGATGATATG
    TCGCGAACTT TGCTAGCTAT GTCTAGCTCC CAAGACAGCT GTATATCCAT GCGACAGTCT
    GGATGTCTTC CTCTCCTCAT CCAGCTTTTA CATGGCAATG ACAAAGACTC TGTGTTGTTG
    GGAAATTCCC GGGGCAGTAA AGAGGCTCGG GCCAGGGCCA GTGCAGCACT CCACAACATC
    ATTCACTCAC AGCCTGATGA CAAGAGAGGC AGGCGTGAAA TCCGAGTCCT TCATCTTTTG
    GAACAGATAC GAGCTTACTG TGAAACCTGT TGGGAGTGGC AGGAAGCCCA TGAACAAGGG
    ATGGACCAGG ACAAAAATCC AATGCCAGCT CCTGTTGAAC ATCAGATCTG CCCTGCTGTC
    TGTGTTCTAA TGAAGCTTTC GTTTGATGAA GAGCATAGAC ATGCCATGAA TGAGCTTGGT
    AGGAAGGCTA CCCGGGGCAT TTCATCCCAG GAGCTAGGGC AGGGGCTTTC AGGGGGACTA
    CAGGCCATTG CAGAATTATT GCAAGTGGAC TGTGAAATGT ATGGGCTTAC TAATGACCAC
    TACAGTATTA CTTTAAGACG ATATGCAGGA ATGGCTTTGA CAAACTTGAC TTTCGGAGAT
    GTAGCCAACA AGGCTACTCT CTGCTCTATG AAAGGCTGCA TGAGAGCGCT CGTGGCTCAG
    CTCCAGTCTG AAAGCGAGGA CTTGCAGCAG GTTATTGCGA GTGTTTTGAG GAATCTGTCT
    TGGAGAGCAG ATGTAAATAG TAAAAAGACT TTGCGTGAAG TTGGAAGTGT GAAAGCATTG
    ATGGAATGTG CTTTGGAAGT GAAAAAGGAA TCAACCCTCA AAAGTGTATT GAGTGCCTTA
    TGGAACTTGT CAGCACACTG CACTGAGAAT AAAGCTGACA TATGTGCTGT GGATGGTGCG
    CTTGCATTTT TGGTTGGCAC TCTCACTTAT CGCAGCCAGA CCAATACTTT AGCCATTATT
    GAAAGTGGAG GTGGTATATT ACGGAATGTA TCCAGCTTGA TAGCTACAAA TGAGGACCAC
    AGGCAAATCC TAAGAGAGAA TAATTGCTTA CAGACCTTAT TACAACACTT GAAATCTCAC
    AGTTTGACAA TAGTCAGCAA TGCATGCGGA ACCTTGTGGA ATCTCTCAGC AAGAAACCCT
    AAAGACCAGG AAGCATTGTG GGACATGGGA GCAGTCAGCA TGCTCAAGAA CCTCATTCAT
    TCAAAGCACA AGATGATTGC TATGGGAAGT GCTGCAGCTT TAAGGAATCT CATGGCAAAT
    AGACCTGCAA AGTATAAAGA TGCCAATATC ATGTCTCCTG GTTCAAGTTT GCCTTCTCTA
    CATGTCAGGA AACAAAAGGC CCTGGAAGCA GAATTAGATG CTCAGCATTT ATCAGAAACT
    TTTGACAATA TTGACAATTT AAGTCCCAAG GCATCTCATC GTAGCAAGCA GAGACACAAG
    CAAAATCTCT ATGGTGACTA TGTTTTTGAC ACCAATCGAC ATGATGATAA CAGGTCAGAC
    AATTTTAATA CTGGAAACAT GACTGTCCTA TCACCGTACT TAAACACTAC AGTATTGCCC
    AGCTCTTCTT CATCAAGGGG AAGTTTAGAT AGCTCTCGTT CTGAGAAAGA TAGAAGTCTG
    GAGAGAGAAC GAGGTATTAG CCTAGGCAAC TATCACCCAG CAACAGAAAA CCCAGGAACC
    TCTTCAAAGC GAGGTTTGCA GATTTCTACC ACTGCAGCCC AGATTGCCAA AGTCATGGAA
    GAAGTATCAG CTATTCATAC CTCTCAGGAA GACAGAAGTT CTGGGTCTAC CACAGAACTA
    CACTGTGGGA CAGATGAGAG GAATGCACTA AGAAGAAGCT CTACCACCCA CACACATGCA
    AACACGTACA ACTTTACCAA GTCAGAAAAC TCAAACAGAA CATGTCCAAT ACCATATGCC
    AAAGTAGAAT ATAAGAGATC TTCAAATGAT AGTTTAAATA GTGTCAGCAG TAGTGATGGT
    TATGGTAAAA GAGGTCAGAT GAAACCTTCA ATTGAATCCT ATTCTGAAGA TGATGAAAGT
    AAATTTTGCA GCTATGGTCA GTATCCAGCT GACCTAGCCC ATAAAATACA TAGTGCAAAT
    CATATGGATG ATAATGATGG AGAACTAGAT ACACCAATAA ATTACAGTCT CAAATATTCC
    GATGAACAGT TGAACTCTGG GAGGCAGAGC CCTTCACAGA ATGAAAGATG GGCAAGACCC
    AAACATATAC TAGAAGATGA AATAAAACCG AATGAGCAGA GACAATCAAG GAGTCAAAGC
    ACAGCTTATC CCGTGTATCC TGAGAGCACT GATGATAAAC ACCTCAAGTT CCAACCACAC
    TTTGGGCAGC AAGAATGTGT TTCCCCATAC AGGTCAAGAG CAGCCAATGG ATCTGAAACA
    AATCGAGTAG GCTCTAATCA TGGAATTAGT CAAAATGTGA ACCAGTCTTT GTGTCAAGAA
    GATGACTATG AAGACGATAA ACCAACCAAC TATAGCGAAC GTTACTCTGA GGAAGGGCAG
    CATGAGGAAG AAGAGAGGCC AACCAATTAT AGCATAAAAT ACAGTGAAGA AAAACATCAC
    GTGGATCAGC CTATTGATTA TAGTTTAAAA TACACCACAG ACATTCCTTC TTCACAGAAA
    CCAGCATTTT CATTCTCAAA GAATTCATCT GGACAGAGCA CAAAAACTGA ACACATCTCT
    TCAAGCAGCG AGAATACATC CACAACTTCA TCTAATGCCA AGAGGCAGAA TCAGCTGCAT
    CCAAGCTCAG CACAGAGCAG AAGTGGTCAG ACCCCAAAAG CCACCTCTTC CTCTTGCAAA
    GTCCCCTCTA TCAACCAAGA AACAATACAG ACTTACTGTG TAGAAGATAC CCCAATATGT
    TTTTCAAGAT GCAGTTCATT ATCATCTCTG TCATCTGCTG AAGATGAAGT AGGGTGTGAT
    CAGACAACAC AAGAAGCAGA GTCTGCTAAC ACTCTGCAAA TAGCTGAAAT CAAGGACAAC
    AGCGGACCTA GGTCAAATGA AGATTCTGTG AGTAAAGTTC CAGCAGGGTC ACAGCACATT
    AGAACCAAAT CCAGCAGACT CCAGGCTTCT GGTCTGTCTT CAGAGTCAGC CAGGCACAAA
    GCTGTTGAAT TTTCTTCAGG GGCCAAATCT CCTTCCAAGA GCGGTGCTCA GACACCCAAA
    AGTCCACCAG AGCACTACGT TCAGGAGACC CCACTCATGT TTAGCAGATG TACTTCAGTC
    AGTTCACTCG ACAGTTTTGA GAGTCGCTCG ATTGCCAGCT CTGTTCAGAG TGAACCCTGC
    AGTGGAATGG TAAGTGGCAT CATAAGCCCC AGTGACCTCC CAGATAGCCC TGGACAAACC
    ATGCCACCAA GCAGAAGCAA AACCCCTCCG CCGCCGCCTC CGCCTCCTCC TCAGACAGTT
    CAAACGAAGC AGGAAGTACC TAAAAATAAA GCACCTAGTG CTGAAAAGAG AGAAAGTGGA
    CCCAAGCAAG CTGCTGTAAA TGCTGCAGTA CAAAGGGTCC AGGTTCTTCC AGAGGCTGAT
    ACTCTGTTAC ATTTTGCCAC AGAGAGTACT CCTGATGGAT TTTCTTGTTC ATCTAGCCTG
    AGCGCTCTGA GCCTTGATGA GCCATTTATT CAGAAAGATG TGGAACTAAG AATAATGCCT
    CCAGTTCAGG AAAATGACAA CGGGAATGAA ACAGAAAGTG AGCAGCCTGA AGAATCAAAT
    GAAAACCAGG AAAAAGAGGC AGAAAAACCC ACTGATTCTG AAAAAGATCT TTTAGATGAG
    TCAGATGATG ATGATATTGA AATACTAGAA GAGTGTATAA TTTCTGCCAT GCCAACAAAA
    TCTTCACGCA AAGCCAAAAA ACCAGCCCAG ACTACTTCAA AATTACCTCC ACCTGTGGCA
    AGGAAACCAA GTCAGCTCCC TGTATACAAA CTTCTGCCAT CGCAAAACAG GTTACAGGCA
    CAAAAGCATG TTAGTTTTAC ACCAGGAGAT GATATGCCAC GGGTGTATTG TGTAGAAGGG
    ACACCTATAA ACTTTTCCAC AGCTACATCT CTGAGTGATC TAACGATAGA ATCCCCTCCA
    AATGAGTTAG CTGCTGGAGA AGGGGTTAGA GCAGGGGCAC AGTCAAGTGA GTTTGAAAAA
    CGAGATACCA TTCCTACTGA AGGCAGAAGT ACAGATGAGG CTCAACGAGG GAAAGCCTCA
    TCTGTCACTG TGCCTGAACT GGACGACAGC AAAACAGAAG AAGGTGATAT TCTTGCAGAA
    TGCATTAATT CTGCCATGCC CAAAGGAAAA AGTCACAAGC CTTTCCGTGT GAAAAAGATC
    ATGGACCAGG TCCAGCAAGC ATCTATGTCT TCATCTGGAA CTAACAAAAA TCAATTAGAT
    GGTAAGACGA AGAAACCTAC TTCACCAGTA AAACCTATAC CACAAAATAC TGAATACAGG
    ACACGTGTAA GAAAAAATAC AGACTCAAAA AATAATCTAA ATGCAGAAAG AAATTTCTCG
    GAAAACAAAG ACTCAAAGAA ACAGCACTTG AAAAATAATT CCAAGGACTT CAATGATAAA
    CTGCCAAATA ATGAAGACAG AGTCAGAGGA AGTTTTACTT TTGATTCACC CCATCATTAC
    ACGCCCATTG AAGGCACTCC GTACTGTTTT TCACGAAATG ACTCTCTGAG TTCTCTAGAT
    TTTGATGATG ATGATGTTGA CCTTTCCAGG GAAAAGGCTG AATTAAGAAA GGGGAAGGAA
    AATAAAGAAT CAGAAGCTAA AGTGACCAAC CACACAGAAC TAACCTCAAA CCAACAATCA
    GCTAGTAAGA CACCAGCTGT TACAAAGCAG CCAATAAATA GAGGTCAGTC TAAACCCGTG
    CTGCAGAAGC AGTCCACTTT TCCCCAGTCT TCCAAAGATA TACCAGACAG AGGGGCAGCA
    ACAGATGAGA AATTACAGAA TTTCGCTATT GAAAATACTC CAGTTTGCTT TTCTCGAAAT
    TCCTCTCTAA GCTCTCTTAG TGACATTGAT CAAGAAAACA ACAACAACAA GGAAAATGAA
    CCTGTCAAAG AGACAGAGCC CCCTGCCTCA CAGGGAGAAC CAGGTAAACC CCAGGCCTCA
    GGTTATGCTC CTAAATCGTT TCACGTGGAA GACACCCCTG TTTGTTTCTC AAGAAACAGT
    TCTCTCAGTT CTCTTAGTAT TGATTCTGAA GATGACCTGC TGCAGGAATG TATAAGTTCT
    GCAATGCCAA AAAAGAAAAA GCCTTCAAGG CTCAAGCCCG ATAATGAAAA GCATAGTCCC
    AGAAATATGG GTGGCATATT AGCAGAAGAT TTGACACTCG ATTTGAAAGA TATACAGAGA
    CCAGATTCAG AACATGGTTT ATCCCCAGAT TCAGAAAATT TTGATTGGAA AGCTATTCAG
    GAAGGTGCAA ATTCCATAGT AAGTAGTTTA CATCAAGCTG CTGCCGCTGC ATGTTTATCT
    CGACAAGCTT CATCTGATTC AGATTCTATC CTTTCATTGA AATCGGGTAT CTCTCTGGGA
    TCACCATTTC ACCTTACACC TGATCAAGAA GAAAAACCCT TTACGAGTAA TAAAGGTCCA
    CGAATTCTAA AACCTGGGGA GAAAAGTACA TTGGAAACTA AAAAAATAGA ATCTGAAAAT
    AAAGGAATAA AAGGAGGCAA AAAGGTTTAT AAAAGTTTGA TTACTGGAAA AGTTCGATCT
    AATTCGGAAA TTTCGAGCCA AATGAAACAA CCCCTTCAAA CAAACATGCC TTCAATCTCT
    CGAGGTAGGA CAATGATTCA TATTCCAGGA GTTCGGAATA GCTCTTCAAG TACAAGCCCA
    GTTTCTAAAA AAGGCCCGCC CCTCAAGACT CCAGCCTCCA AAAGCCCTAG TGAAGGTCAG
    CCGGCTACCA CCTCTCCCAG AGGAACCAAG CCATCAGTGA AGTCAGAATT AAGCCCTGTT
    ACAAGGCAGG CATCCCAGAC AGCAGGATCA AACAAAGGAC CTTCTAGATC AGGATCTAGA
    GATTCCACTC CTTCAAGACC TGCCCAGCAA CCATTAAGTA GACCAATGCA GTCTCCAGGG
    CGAAACTCAA TCTCTCCTGG TAGAAATGGA ATAAGTCCCC CTAACAAATT ATCTCAACTA
    CCAAGGACGT CATCCCCTAG TACTGCTTCA ACTAAGTCCT CAGGTTCTGG GAAAATGTCT
    TACACATCTC CTGGCAGACA GATGAGCCAG CAGAACCTCA CCAAACAAAC AGGCTTATCC
    AAGAATGGCA GTGGTATCCC AAGAAGTGAA TCTGCCTCCA AAGGGCTAAA TCAAATGAGT
    AATAGTAATG GATCCAATAA AAAAGTAGAA CTTTCTAGAA TGTCTTCAAC AAAGTCAAGT
    GGAAGTGAAT CCGATAGGTC AGAGAGACCT GTATTAGTAC GCCAATCAAC TTTCATCAAA
    GAAGCTCCAA GCCCAACCCT AAGGAGAAAA CTGGAGGAAT CTGCTTCATT TGAATCTCTT
    TCTCCATCTT CCAGACCCGA TTCTCCCACA CGGTCCCAGG CTCATACTCC AGTTTTAAGT
    CCTTCCCTTC CTGATATGTC TCTATCTACA CATTCGTCTC TTCAGTCTGG TGGATGGCGA
    AAACTCCCAC CTAACCTCAG TCCCACCATA GAGTATAATG ATGGAAGACC AGTAAAGCGC
    CATGATATAG CACGCTCTCA TTCCGAAAGT CCTTCCAGAC TTCCCATCAA TAGGTCAGGG
    ACCTGGAAAC GTGAGCACAG CAAACACTCA TCATCACTTC CTCGAGTAAG CACTTGGAGA
    AGAACTGGAA GTTCATCCTC AATTCTTTCT GCTTCATCAG AATCTAGTGA AAAGGCAAAA
    AGTGAGGACG AAAAACAAGT GAACTCTATT TCAGGAAGCA AACAAACTAA AGAAAACCAG
    GTATCCACAA AAGGAACATG GAGAAAAATA AAAGAAAGTG AAATTTCTCC CACAAATAGT
    ACTTCTCAGA CCACTTCTTC AGGTGCTGCA AATGGTGCTG AATCAAAGAC TCTGATTTAT
    CAAATGGCAC CTGCTGTTTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGATTGCCCC
    ATTAACAACC CTAGATCTGG AAGATCTCCA ACAGGAAATA CTCCCCCCGT GATTGACACT
    GTTTCAGAAA AGGGAAACCC GAACCCTAAA GATTCAAAAG ATAATCAGGG AAAACAAAAT
    GTGAGCAATG GTAGTGCCCC TACACGCACC ATGGGTCTGG AAAACCGCCT GAATTCCTTT
    ATTCAGGTAG ATCCTCCAGA CCAAAAAGGA ACTGAGACAA AACCGGGACA CAGTAATAAC
    CCTGTCCCTG CATCCGAGAC TAGTGAAAGT TCTATAGCCG AGCGTACCCC GTTTAGTTCT
    AGCAGCTCAA GCAAGCACAG TTCACCAAGT GGGACTGTCG CCGCCAGGGT GAGTCCTTTT
    AATTACAACC CAAGCCCAAG GAAGAGCAGC ACAGATGGCA CTTCAGCCCG ACCGTCTCAG
    ATCCCAACGC CAGTGAGCAA CAACACAAAG AAACGCGACT CAAAACCCGA CAGCACAGAA
    CCCAGCGGGA CTCAAAGTCC TAAACGCCAT TCTGGGTCTT ACCTTGTGAC ATCTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.