RASGRP1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRP1
Entrez Gene ID	533125
Full Name	RAS guanyl releasing protein 1
General protein information	Preferred Names RAS guanyl releasing protein 1 Names RAS guanyl-releasing protein 1 RAS guanyl releasing protein 1 (calcium and DAG-regulated)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005211584.2	XP_005211641.1	RAS guanyl-releasing protein 1 isoform X1
XM_005211584.3	XP_005211641.1	RAS guanyl-releasing protein 1 isoform X1
XM_015473118.1	XP_015328604.1	RAS guanyl-releasing protein 1 isoform X2
NM_001144078.1	NP_001137550.1	RAS guanyl-releasing protein 1
XM_024997639.1	XP_024853407.1	RAS guanyl-releasing protein 1 isoform X2
XM_015473118.2	XP_015328604.1	RAS guanyl-releasing protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RASGRP1	XP_510293.2
Canis lupus familiaris (dog)	RASGRP1	XP_005638732.1
Rattus norvegicus (Norway rat)	Rasgrp1	NP_062084.2
Mus musculus (house mouse)	Rasgrp1	NP_035376.1
Homo sapiens (human)	RASGRP1	NP_005730.2
	RASGRP1	XP_001092164.1
Bos taurus (cattle)	RASGRP1	NP_001137550.1
Gallus gallus (chicken)	RASGRP1	XP_004941790.1
Danio rerio (zebrafish)	rasgrp4	NP_001038327.1
Xenopus tropicalis (tropical clawed frog)	rasgrp1	NP_001093748.2

Gene Ontology
Bibliography

Related articles in PubMed

Segregation of the hereditary thrombopathia-associated polymorphism in polled German Fleckvieh cattle.
Zerbin I, Metzger J, Dierks C, Distl O
Animal genetics46(5)584-5(2015 Oct)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a mutation in RASGRP1 is associated with hereditary thrombopathia in polled German Fleckvieh cattle
Title: Segregation of the hereditary thrombopathia-associated polymorphism in polled German Fleckvieh cattle.

The following RASGRP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40881	NM_001144078.1 Latest version!	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa23169	XM_005211584.3 Latest version!	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa91667	XM_015473118.2 Latest version!	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa91667	XM_024997639.1 Latest version!	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa23169	XM_005211584.2	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa91667	XM_015473118.1	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRP1 ( NM_001144078.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137550.1 Bos taurus RAS guanyl releasing protein 1 (RASGRP1), mRNA.

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CloneID	OBa40881
Clone ID Related Accession (Same CDS sequence)	NM_001144078.1
Accession Version	NM_001144078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	RAS guanyl-releasing protein 1
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000010.1. On Jan 22, 2009 this sequence version replaced XM_001253077.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.12299.1, SRR5571254.44949.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGCACCC TGGGCAAAGC GAGAGAAGCT CCACGGAAAC CAACTCATGG CTGCAGAGCA 
GTCCCTAAAG CGAGACTAGA GGCGAAGCCC GCCAGCAGCC CCCTCCCCTG CCATCCCGGC 
CTGGCCCAGA TCACCCAGTT CCGAATGATG GTGCCTCTGG GACATTTTGC CAAAGGAGCC 
AGCCTGGACG ATCTCATCGA CAGCTGTGTT CAATCTTTTG ATGCAGACGG AAACCTGTGT 
CGAAGTAACC AACTGTTGCA AGTCATGCTG ACCATGCACC GAATTCTCAT TTCCTCTGCT 
GAACTGCTCC AAAAAGTTAT CACTCTCTAT AAGGATGCCT TGGCAAAGAA TTCACCAGGG 
CTTTGCCTGA AGATCTGCTA TTTTGTAAGG TACTGGATAA CAGAATTCTG GATCATGTTT 
AAGATGGATA CCAGCCTGGC AAGCACCATG GAGGAGTTTC AGGAACTGGT GAAAGCAAAT 
GGCGAGGAGC TCCACTGCCG CCTGATCGAC ACAACGCAAA TCAATGCCCG TGACTGGTCC 
AGGAAACTGA CCCAAAGGAT AAAATCGAAC ACCAGCAAGA AGCGGAAAGT CTCCCTGCTC 
TTTGACCATC TGGAGCCAGA AGAACTGTCT GAGCACCTGA CTTTCCTAGA ATTCAAGTCC 
TTCCGGAGGA TATCGTTCTC TGATTATCAG AATTACCTTG TGAACAGCTG CGTGAAGGAG 
AACCCCACCA TGGAGCGGTC CATTGCTCTG TGCAACGGCA TCTCCCAGTG GGTGCAGCTG 
ATGGTTCTCA GCCGCCCCAC CCCCCAGCTC CGAGCTGAAG TCTTCATCAA GTTCATCCAG 
GTGGCTCAGA AGCTCCATCA GCTGCAGAAC TTCAATACGT TGATGGCCGT GATCGGAGGG 
TTATGTCACA GCTCTATTTC CAGGCTCAAG GAGACAAGTT CACATGTCCC GCATGAAATC 
AATAAGGTTC TAGGTGAGAT GACTGAGCTG CTGTCCTCCT GTAGAAACTA CGACAACTAC 
CGGCGTGCTT ATGGGGAGTG CACCGACTTC AAGATCCCCA TCCTGGGGGT GCACCTCAAG 
GACCTCATCT CCCTGTACGA AGCCATGCCT GACTACCTGG AGGAGGGGAA GGTGAACGTC 
CATAAGCTGC TGGCCCTGTA CAACCACATC AATGAGTTAG TCCAGCTACA GGAGGTGCCC 
CCGCCCCTGG AGGCCAATAA GGACCTGGTG CACCTGCTGA CGTTATCCCT AGATCTCTAC 
TACACTGAAG ATGAAATCTA CGAACTTTCC TATGCCCGGG AACCCAGGAA TCACAAAGCC 
CCACCACTAA CACCTTCAAA GCCACCAGTA GTAGTGGACT GGGCCTCTGG AGTATCTCCC 
AAGCCTGATC CAAAGACCAT AAGCAAACAC GTCCAGAGGA TGGTGGATTC TGTCTTCAAG 
AACTATGATC ATGACCAGGA TGGATACATT TCCCAGGAAG AATTTGAAAA GATTGCTGCA 
AGTTTTCCAT TTTCCTTCTG TGTGATGGAT AAAGACAGGG AAGGCCTCAT CAGCAGAGAC 
GAGATCACGG CCTACTTCAT GAGGGCCAGC TCCATCTACT CCAAGTTGGG CCTCGGTTTT 
CCTCACAACT TCCAAGAGAC CACCTACCTG AAGCCTACTT TCTGTGACAA CTGTGCTGGA 
TTTCTCTGGG GAGTGATCAA ACAAGGGTAT CGGTGTAAAG ACTGTGGGAT GAACTGCCAC 
AAACAATGCA AAGACCTGGT TGTGTTCGAA TGCAAGAAGC GAGCCAAGAA CTCAACAGCT 
CCCACAGAGA TCAGCACCTC TGTGGGGCCA ACGTCCAACC TTTGCTCCTT GGGAGCCAAA 
GATCTGCTCC ATGCACCTGA GGAAGGACCT TTCACATTCC CTAACGGGGA GGCTGCGGAA 
CACAGTGAGG AGAGTAAGGA TCGGACCATC ATGCTCATGG GAGTATCCTC ACAGAAGATT 
TCTGTTCGGC TGAAGAGGAC TGTCGCCCAC ATGGCCACCC AGACTGAAGC ACTGTCTTGG 
CCTGGCAGTG AGGGCCCTTC CAGTCACTTT GTGCTGTCAT CCCCAAGGAA GACAGCCCAG 
GATACTCTGT ATGTACTGCC CAGCCCTACA TCTCCGTGCC CCAGCCCAGT CTTGGTCAGG 
AAGCGGGCTT TCGTCAAGTG GGAGAACAAA GAATCCTTCA TAAAATCAAA GGAGGAGCTC 
CGTCACCTCA GGCTTCCAAC ATACCAAGAG CTGGAACAGG AAATAAATAC CCTGAAAGCA 
GATAATAATG CTCTAAAGAT CCAACTGAAA TATGCACAGA AGAAGATAGA AACCCTCCAA 
CTTGCAAGAA ACAATCATGT CTTGGCTCAG ATGGAGCAGG GTGACTGTTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137550.1
CDS	97..2490
Translation	MGTLGKAREAPRKPTHGCRAVPKARLEAKPASSPLPCHPGLAQITQFRMMVPLGHFAKGASLDDLIDSCVQSFDADGNLCRSNQLLQVMLTMHRILISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWIMFKMDTSLASTMEEFQELVKANGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTFLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEEGKVNVHKLLALYNHINELVQLQEVPPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHKAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNSTAPTEISTSVGPTSNLCSLGAKDLLHAPEEGPFTFPNGEAAEHSEESKDRTIMLMGVSSQKISVRLKRTVAHMATQTEALSWPGSEGPSSHFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKESFIKSKEELRHLRLPTYQELEQEINTLKADNNALKIQLKYAQKKIETLQLARNNHVLAQMEQGDCS

Target ORF information:

RefSeq Version	NM_001144078.1
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144078.1

ORF Insert Sequence:

ATGGGCACCC TGGGCAAAGC GAGAGAAGCT CCACGGAAAC CAACTCATGG CTGCAGAGCA 
GTCCCTAAAG CGAGACTAGA GGCGAAGCCC GCCAGCAGCC CCCTCCCCTG CCATCCCGGC 
CTGGCCCAGA TCACCCAGTT CCGAATGATG GTGCCTCTGG GACATTTTGC CAAAGGAGCC 
AGCCTGGACG ATCTCATCGA CAGCTGTGTT CAATCTTTTG ATGCAGACGG AAACCTGTGT 
CGAAGTAACC AACTGTTGCA AGTCATGCTG ACCATGCACC GAATTCTCAT TTCCTCTGCT 
GAACTGCTCC AAAAAGTTAT CACTCTCTAT AAGGATGCCT TGGCAAAGAA TTCACCAGGG 
CTTTGCCTGA AGATCTGCTA TTTTGTAAGG TACTGGATAA CAGAATTCTG GATCATGTTT 
AAGATGGATA CCAGCCTGGC AAGCACCATG GAGGAGTTTC AGGAACTGGT GAAAGCAAAT 
GGCGAGGAGC TCCACTGCCG CCTGATCGAC ACAACGCAAA TCAATGCCCG TGACTGGTCC 
AGGAAACTGA CCCAAAGGAT AAAATCGAAC ACCAGCAAGA AGCGGAAAGT CTCCCTGCTC 
TTTGACCATC TGGAGCCAGA AGAACTGTCT GAGCACCTGA CTTTCCTAGA ATTCAAGTCC 
TTCCGGAGGA TATCGTTCTC TGATTATCAG AATTACCTTG TGAACAGCTG CGTGAAGGAG 
AACCCCACCA TGGAGCGGTC CATTGCTCTG TGCAACGGCA TCTCCCAGTG GGTGCAGCTG 
ATGGTTCTCA GCCGCCCCAC CCCCCAGCTC CGAGCTGAAG TCTTCATCAA GTTCATCCAG 
GTGGCTCAGA AGCTCCATCA GCTGCAGAAC TTCAATACGT TGATGGCCGT GATCGGAGGG 
TTATGTCACA GCTCTATTTC CAGGCTCAAG GAGACAAGTT CACATGTCCC GCATGAAATC 
AATAAGGTTC TAGGTGAGAT GACTGAGCTG CTGTCCTCCT GTAGAAACTA CGACAACTAC 
CGGCGTGCTT ATGGGGAGTG CACCGACTTC AAGATCCCCA TCCTGGGGGT GCACCTCAAG 
GACCTCATCT CCCTGTACGA AGCCATGCCT GACTACCTGG AGGAGGGGAA GGTGAACGTC 
CATAAGCTGC TGGCCCTGTA CAACCACATC AATGAGTTAG TCCAGCTACA GGAGGTGCCC 
CCGCCCCTGG AGGCCAATAA GGACCTGGTG CACCTGCTGA CGTTATCCCT AGATCTCTAC 
TACACTGAAG ATGAAATCTA CGAACTTTCC TATGCCCGGG AACCCAGGAA TCACAAAGCC 
CCACCACTAA CACCTTCAAA GCCACCAGTA GTAGTGGACT GGGCCTCTGG AGTATCTCCC 
AAGCCTGATC CAAAGACCAT AAGCAAACAC GTCCAGAGGA TGGTGGATTC TGTCTTCAAG 
AACTATGATC ATGACCAGGA TGGATACATT TCCCAGGAAG AATTTGAAAA GATTGCTGCA 
AGTTTTCCAT TTTCCTTCTG TGTGATGGAT AAAGACAGGG AAGGCCTCAT CAGCAGAGAC 
GAGATCACGG CCTACTTCAT GAGGGCCAGC TCCATCTACT CCAAGTTGGG CCTCGGTTTT 
CCTCACAACT TCCAAGAGAC CACCTACCTG AAGCCTACTT TCTGTGACAA CTGTGCTGGA 
TTTCTCTGGG GAGTGATCAA ACAAGGGTAT CGGTGTAAAG ACTGTGGGAT GAACTGCCAC 
AAACAATGCA AAGACCTGGT TGTGTTCGAA TGCAAGAAGC GAGCCAAGAA CTCAACAGCT 
CCCACAGAGA TCAGCACCTC TGTGGGGCCA ACGTCCAACC TTTGCTCCTT GGGAGCCAAA 
GATCTGCTCC ATGCACCTGA GGAAGGACCT TTCACATTCC CTAACGGGGA GGCTGCGGAA 
CACAGTGAGG AGAGTAAGGA TCGGACCATC ATGCTCATGG GAGTATCCTC ACAGAAGATT 
TCTGTTCGGC TGAAGAGGAC TGTCGCCCAC ATGGCCACCC AGACTGAAGC ACTGTCTTGG 
CCTGGCAGTG AGGGCCCTTC CAGTCACTTT GTGCTGTCAT CCCCAAGGAA GACAGCCCAG 
GATACTCTGT ATGTACTGCC CAGCCCTACA TCTCCGTGCC CCAGCCCAGT CTTGGTCAGG 
AAGCGGGCTT TCGTCAAGTG GGAGAACAAA GAATCCTTCA TAAAATCAAA GGAGGAGCTC 
CGTCACCTCA GGCTTCCAAC ATACCAAGAG CTGGAACAGG AAATAAATAC CCTGAAAGCA 
GATAATAATG CTCTAAAGAT CCAACTGAAA TATGCACAGA AGAAGATAGA AACCCTCCAA 
CTTGCAAGAA ACAATCATGT CTTGGCTCAG ATGGAGCAGG GTGACTGTTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRP1 ( XM_005211584.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211641.1 Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23169
Clone ID Related Accession (Same CDS sequence)	XM_005211584.2 , XM_005211584.3
Accession Version	XM_005211584.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	RAS guanyl-releasing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005211584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCC TGGGCAAAGC GAGAGAAGCT CCACGGAAAC CAACTCATGG CTGCAGAGCA 
GTCCCTAAAG CGAGACTAGA GGCGAAGCCC GCCAGCAGCC CCCTCCCCTG CCATCCCGGC 
CTGGCCCAGA TCACCCAGTT CCGAATGATG GTGCCTCTGG GACATTTTGC CAAAGGAGCC 
AGCCTGGACG ATCTCATCGA CAGCTGTGTT CAATCTTTTG ATGCAGACGG AAACCTGTGT 
CGAAGTAACC AACTGTTGCA AGTCATGCTG ACCATGCACC GAATTCTCAT TTCCTCTGCT 
GAACTGCTCC AAAAAGTTAT CACTCTCTAT AAGGATGCCT TGGCAAAGAA TTCACCAGGG 
CTTTGCCTGA AGATCTGCTA TTTTGTAAGG TACTGGATAA CAGAATTCTG GATCATGTTT 
AAGATGGATA CCAGCCTGGC AAGCACCATG GAGGAGTTTC AGGAACTGGT GAAAGCAAAT 
GGCGAGGAGC TCCACTGCCG CCTGATCGAC ACAACGCAAA TCAATGCCCG TGACTGGTCC 
AGGAAACTGA CCCAAAGGAT AAAATCGAAC ACCAGCAAGA AGCGGAAAGT CTCCCTGCTC 
TTTGACCATC TGGAGCCAGA AGAACTGTCT GAGCACCTGA CTTTCCTAGA ATTCAAGTCC 
TTCCGGAGGA TATCGTTCTC TGATTATCAG AATTACCTTG TGAACAGCTG CGTGAAGGAG 
AACCCCACCA TGGAGCGGTC CATTGCTCTG TGCAACGGCA TCTCCCAGTG GGTGCAGCTG 
ATGGTTCTCA GCCGCCCCAC CCCCCAGCTC CGAGCTGAAG TCTTCATCAA GTTCATCCAG 
GTGGCTCAGA AGCTCCATCA GCTGCAGAAC TTCAATACGT TGATGGCCGT GATCGGAGGG 
TTATGTCACA GCTCTATTTC CAGGCTCAAG GAGACAAGTT CACATGTCCC GCATGAAATC 
AATAAGGTTC TAGGTGAGAT GACTGAGCTG CTGTCCTCCT GTAGAAACTA CGACAACTAC 
CGGCGTGCTT ATGGGGAGTG CACCGACTTC AAGATCCCCA TCCTGGGGGT GCACCTCAAG 
GACCTCATCT CCCTGTACGA AGCCATGCCT GACTACCTGG AGGAGGGGAA GGTGAACGTC 
CATAAGCTGC TGGCCCTGTA CAACCACATC AATGAGTTAG TCCAGCTACA GGAGGTGCCC 
CCGCCCCTGG AGGCCAATAA GGACCTGGTG CACCTGCTGA CGTTATCCCT AGATCTCTAC 
TACACTGAAG ATGAAATCTA CGAACTTTCC TATGCCCGGG AACCCAGGAA TCACAAAGCC 
CCATCTGTCT TCAAGAACTA TGATCATGAC CAGGATGGAT ACATTTCCCA GGAAGAATTT 
GAAAAGATTG CTGCAAGTTT TCCATTTTCC TTCTGTGTGA TGGATAAAGA CAGGGAAGGC 
CTCATCAGCA GAGACGAGAT CACGGCCTAC TTCATGAGGG CCAGCTCCAT CTACTCCAAG 
TTGGGCCTCG GTTTTCCTCA CAACTTCCAA GAGACCACCT ACCTGAAGCC TACTTTCTGT 
GACAACTGTG CTGGATTTCT CTGGGGAGTG ATCAAACAAG GGTATCGGTG TAAAGACTGT 
GGGATGAACT GCCACAAACA ATGCAAAGAC CTGGTTGTGT TCGAATGCAA GAAGCGAGCC 
AAGAACTCAA CAGCTCCCAC AGAGATCAGC ACCTCTGTGG GGCCAACGTC CAACCTTTGC 
TCCTTGGGAG CCAAAGATCT GCTCCATGCA CCTGAGGAAG GACCTTTCAC ATTCCCTAAC 
GGGGAGGCTG CGGAACACAG TGAGGAGAGT AAGGATCGGA CCATCATGCT CATGGGAGTA 
TCCTCACAGA AGATTTCTGT TCGGCTGAAG AGGACTGTCG CCCACATGGC CACCCAGACT 
GAAGCACTGT CTTGGCCTGG CAGTGAGGGC CCTTCCAGTC ACTTTGTGCT GTCATCCCCA 
AGGAAGACAG CCCAGGATAC TCTGTATGTA CTGCCCAGCC CTACATCTCC GTGCCCCAGC 
CCAGTCTTGG TCAGGAAGCG GGCTTTCGTC AAGTGGGAGA ACAAAGAATC CTTCATAAAA 
TCAAAGGAGG AGCTCCGTCA CCTCAGGCTT CCAACATACC AAGAGCTGGA ACAGGAAATA 
AATACCCTGA AAGCAGATAA TAATGCTCTA AAGATCCAAC TGAAATATGC ACAGAAGAAG 
ATAGAAACCC TCCAACTTGC AAGAAACAAT CATGTCTTGG CTCAGATGGA GCAGGGTGAC 
TGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211641.1
CDS	85..2373
Translation	MGTLGKAREAPRKPTHGCRAVPKARLEAKPASSPLPCHPGLAQITQFRMMVPLGHFAKGASLDDLIDSCVQSFDADGNLCRSNQLLQVMLTMHRILISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWIMFKMDTSLASTMEEFQELVKANGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTFLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEEGKVNVHKLLALYNHINELVQLQEVPPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHKAPSVFKNYDHDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNSTAPTEISTSVGPTSNLCSLGAKDLLHAPEEGPFTFPNGEAAEHSEESKDRTIMLMGVSSQKISVRLKRTVAHMATQTEALSWPGSEGPSSHFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKESFIKSKEELRHLRLPTYQELEQEINTLKADNNALKIQLKYAQKKIETLQLARNNHVLAQMEQGDCS

Target ORF information:

RefSeq Version	XM_005211584.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211584.2

ORF Insert Sequence:

ATGGGCACCC TGGGCAAAGC GAGAGAAGCT CCACGGAAAC CAACTCATGG CTGCAGAGCA 
GTCCCTAAAG CGAGACTAGA GGCGAAGCCC GCCAGCAGCC CCCTCCCCTG CCATCCCGGC 
CTGGCCCAGA TCACCCAGTT CCGAATGATG GTGCCTCTGG GACATTTTGC CAAAGGAGCC 
AGCCTGGACG ATCTCATCGA CAGCTGTGTT CAATCTTTTG ATGCAGACGG AAACCTGTGT 
CGAAGTAACC AACTGTTGCA AGTCATGCTG ACCATGCACC GAATTCTCAT TTCCTCTGCT 
GAACTGCTCC AAAAAGTTAT CACTCTCTAT AAGGATGCCT TGGCAAAGAA TTCACCAGGG 
CTTTGCCTGA AGATCTGCTA TTTTGTAAGG TACTGGATAA CAGAATTCTG GATCATGTTT 
AAGATGGATA CCAGCCTGGC AAGCACCATG GAGGAGTTTC AGGAACTGGT GAAAGCAAAT 
GGCGAGGAGC TCCACTGCCG CCTGATCGAC ACAACGCAAA TCAATGCCCG TGACTGGTCC 
AGGAAACTGA CCCAAAGGAT AAAATCGAAC ACCAGCAAGA AGCGGAAAGT CTCCCTGCTC 
TTTGACCATC TGGAGCCAGA AGAACTGTCT GAGCACCTGA CTTTCCTAGA ATTCAAGTCC 
TTCCGGAGGA TATCGTTCTC TGATTATCAG AATTACCTTG TGAACAGCTG CGTGAAGGAG 
AACCCCACCA TGGAGCGGTC CATTGCTCTG TGCAACGGCA TCTCCCAGTG GGTGCAGCTG 
ATGGTTCTCA GCCGCCCCAC CCCCCAGCTC CGAGCTGAAG TCTTCATCAA GTTCATCCAG 
GTGGCTCAGA AGCTCCATCA GCTGCAGAAC TTCAATACGT TGATGGCCGT GATCGGAGGG 
TTATGTCACA GCTCTATTTC CAGGCTCAAG GAGACAAGTT CACATGTCCC GCATGAAATC 
AATAAGGTTC TAGGTGAGAT GACTGAGCTG CTGTCCTCCT GTAGAAACTA CGACAACTAC 
CGGCGTGCTT ATGGGGAGTG CACCGACTTC AAGATCCCCA TCCTGGGGGT GCACCTCAAG 
GACCTCATCT CCCTGTACGA AGCCATGCCT GACTACCTGG AGGAGGGGAA GGTGAACGTC 
CATAAGCTGC TGGCCCTGTA CAACCACATC AATGAGTTAG TCCAGCTACA GGAGGTGCCC 
CCGCCCCTGG AGGCCAATAA GGACCTGGTG CACCTGCTGA CGTTATCCCT AGATCTCTAC 
TACACTGAAG ATGAAATCTA CGAACTTTCC TATGCCCGGG AACCCAGGAA TCACAAAGCC 
CCATCTGTCT TCAAGAACTA TGATCATGAC CAGGATGGAT ACATTTCCCA GGAAGAATTT 
GAAAAGATTG CTGCAAGTTT TCCATTTTCC TTCTGTGTGA TGGATAAAGA CAGGGAAGGC 
CTCATCAGCA GAGACGAGAT CACGGCCTAC TTCATGAGGG CCAGCTCCAT CTACTCCAAG 
TTGGGCCTCG GTTTTCCTCA CAACTTCCAA GAGACCACCT ACCTGAAGCC TACTTTCTGT 
GACAACTGTG CTGGATTTCT CTGGGGAGTG ATCAAACAAG GGTATCGGTG TAAAGACTGT 
GGGATGAACT GCCACAAACA ATGCAAAGAC CTGGTTGTGT TCGAATGCAA GAAGCGAGCC 
AAGAACTCAA CAGCTCCCAC AGAGATCAGC ACCTCTGTGG GGCCAACGTC CAACCTTTGC 
TCCTTGGGAG CCAAAGATCT GCTCCATGCA CCTGAGGAAG GACCTTTCAC ATTCCCTAAC 
GGGGAGGCTG CGGAACACAG TGAGGAGAGT AAGGATCGGA CCATCATGCT CATGGGAGTA 
TCCTCACAGA AGATTTCTGT TCGGCTGAAG AGGACTGTCG CCCACATGGC CACCCAGACT 
GAAGCACTGT CTTGGCCTGG CAGTGAGGGC CCTTCCAGTC ACTTTGTGCT GTCATCCCCA 
AGGAAGACAG CCCAGGATAC TCTGTATGTA CTGCCCAGCC CTACATCTCC GTGCCCCAGC 
CCAGTCTTGG TCAGGAAGCG GGCTTTCGTC AAGTGGGAGA ACAAAGAATC CTTCATAAAA 
TCAAAGGAGG AGCTCCGTCA CCTCAGGCTT CCAACATACC AAGAGCTGGA ACAGGAAATA 
AATACCCTGA AAGCAGATAA TAATGCTCTA AAGATCCAAC TGAAATATGC ACAGAAGAAG 
ATAGAAACCC TCCAACTTGC AAGAAACAAT CATGTCTTGG CTCAGATGGA GCAGGGTGAC 
TGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRP1 ( XM_005211584.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211641.1 Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23169
Clone ID Related Accession (Same CDS sequence)	XM_005211584.2 , XM_005211584.3
Accession Version	XM_005211584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RAS guanyl-releasing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCC TGGGCAAAGC GAGAGAAGCT CCACGGAAAC CAACTCATGG CTGCAGAGCA 
GTCCCTAAAG CGAGACTAGA GGCGAAGCCC GCCAGCAGCC CCCTCCCCTG CCATCCCGGC 
CTGGCCCAGA TCACCCAGTT CCGAATGATG GTGCCTCTGG GACATTTTGC CAAAGGAGCC 
AGCCTGGACG ATCTCATCGA CAGCTGTGTT CAATCTTTTG ATGCAGACGG AAACCTGTGT 
CGAAGTAACC AACTGTTGCA AGTCATGCTG ACCATGCACC GAATTCTCAT TTCCTCTGCT 
GAACTGCTCC AAAAAGTTAT CACTCTCTAT AAGGATGCCT TGGCAAAGAA TTCACCAGGG 
CTTTGCCTGA AGATCTGCTA TTTTGTAAGG TACTGGATAA CAGAATTCTG GATCATGTTT 
AAGATGGATA CCAGCCTGGC AAGCACCATG GAGGAGTTTC AGGAACTGGT GAAAGCAAAT 
GGCGAGGAGC TCCACTGCCG CCTGATCGAC ACAACGCAAA TCAATGCCCG TGACTGGTCC 
AGGAAACTGA CCCAAAGGAT AAAATCGAAC ACCAGCAAGA AGCGGAAAGT CTCCCTGCTC 
TTTGACCATC TGGAGCCAGA AGAACTGTCT GAGCACCTGA CTTTCCTAGA ATTCAAGTCC 
TTCCGGAGGA TATCGTTCTC TGATTATCAG AATTACCTTG TGAACAGCTG CGTGAAGGAG 
AACCCCACCA TGGAGCGGTC CATTGCTCTG TGCAACGGCA TCTCCCAGTG GGTGCAGCTG 
ATGGTTCTCA GCCGCCCCAC CCCCCAGCTC CGAGCTGAAG TCTTCATCAA GTTCATCCAG 
GTGGCTCAGA AGCTCCATCA GCTGCAGAAC TTCAATACGT TGATGGCCGT GATCGGAGGG 
TTATGTCACA GCTCTATTTC CAGGCTCAAG GAGACAAGTT CACATGTCCC GCATGAAATC 
AATAAGGTTC TAGGTGAGAT GACTGAGCTG CTGTCCTCCT GTAGAAACTA CGACAACTAC 
CGGCGTGCTT ATGGGGAGTG CACCGACTTC AAGATCCCCA TCCTGGGGGT GCACCTCAAG 
GACCTCATCT CCCTGTACGA AGCCATGCCT GACTACCTGG AGGAGGGGAA GGTGAACGTC 
CATAAGCTGC TGGCCCTGTA CAACCACATC AATGAGTTAG TCCAGCTACA GGAGGTGCCC 
CCGCCCCTGG AGGCCAATAA GGACCTGGTG CACCTGCTGA CGTTATCCCT AGATCTCTAC 
TACACTGAAG ATGAAATCTA CGAACTTTCC TATGCCCGGG AACCCAGGAA TCACAAAGCC 
CCATCTGTCT TCAAGAACTA TGATCATGAC CAGGATGGAT ACATTTCCCA GGAAGAATTT 
GAAAAGATTG CTGCAAGTTT TCCATTTTCC TTCTGTGTGA TGGATAAAGA CAGGGAAGGC 
CTCATCAGCA GAGACGAGAT CACGGCCTAC TTCATGAGGG CCAGCTCCAT CTACTCCAAG 
TTGGGCCTCG GTTTTCCTCA CAACTTCCAA GAGACCACCT ACCTGAAGCC TACTTTCTGT 
GACAACTGTG CTGGATTTCT CTGGGGAGTG ATCAAACAAG GGTATCGGTG TAAAGACTGT 
GGGATGAACT GCCACAAACA ATGCAAAGAC CTGGTTGTGT TCGAATGCAA GAAGCGAGCC 
AAGAACTCAA CAGCTCCCAC AGAGATCAGC ACCTCTGTGG GGCCAACGTC CAACCTTTGC 
TCCTTGGGAG CCAAAGATCT GCTCCATGCA CCTGAGGAAG GACCTTTCAC ATTCCCTAAC 
GGGGAGGCTG CGGAACACAG TGAGGAGAGT AAGGATCGGA CCATCATGCT CATGGGAGTA 
TCCTCACAGA AGATTTCTGT TCGGCTGAAG AGGACTGTCG CCCACATGGC CACCCAGACT 
GAAGCACTGT CTTGGCCTGG CAGTGAGGGC CCTTCCAGTC ACTTTGTGCT GTCATCCCCA 
AGGAAGACAG CCCAGGATAC TCTGTATGTA CTGCCCAGCC CTACATCTCC GTGCCCCAGC 
CCAGTCTTGG TCAGGAAGCG GGCTTTCGTC AAGTGGGAGA ACAAAGAATC CTTCATAAAA 
TCAAAGGAGG AGCTCCGTCA CCTCAGGCTT CCAACATACC AAGAGCTGGA ACAGGAAATA 
AATACCCTGA AAGCAGATAA TAATGCTCTA AAGATCCAAC TGAAATATGC ACAGAAGAAG 
ATAGAAACCC TCCAACTTGC AAGAAACAAT CATGTCTTGG CTCAGATGGA GCAGGGTGAC 
TGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211641.1
CDS	1..2289
Translation	MGTLGKAREAPRKPTHGCRAVPKARLEAKPASSPLPCHPGLAQITQFRMMVPLGHFAKGASLDDLIDSCVQSFDADGNLCRSNQLLQVMLTMHRILISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWIMFKMDTSLASTMEEFQELVKANGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTFLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEEGKVNVHKLLALYNHINELVQLQEVPPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHKAPSVFKNYDHDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNSTAPTEISTSVGPTSNLCSLGAKDLLHAPEEGPFTFPNGEAAEHSEESKDRTIMLMGVSSQKISVRLKRTVAHMATQTEALSWPGSEGPSSHFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKESFIKSKEELRHLRLPTYQELEQEINTLKADNNALKIQLKYAQKKIETLQLARNNHVLAQMEQGDCS

Target ORF information:

RefSeq Version	XM_005211584.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211584.3

ORF Insert Sequence:

ATGGGCACCC TGGGCAAAGC GAGAGAAGCT CCACGGAAAC CAACTCATGG CTGCAGAGCA 
GTCCCTAAAG CGAGACTAGA GGCGAAGCCC GCCAGCAGCC CCCTCCCCTG CCATCCCGGC 
CTGGCCCAGA TCACCCAGTT CCGAATGATG GTGCCTCTGG GACATTTTGC CAAAGGAGCC 
AGCCTGGACG ATCTCATCGA CAGCTGTGTT CAATCTTTTG ATGCAGACGG AAACCTGTGT 
CGAAGTAACC AACTGTTGCA AGTCATGCTG ACCATGCACC GAATTCTCAT TTCCTCTGCT 
GAACTGCTCC AAAAAGTTAT CACTCTCTAT AAGGATGCCT TGGCAAAGAA TTCACCAGGG 
CTTTGCCTGA AGATCTGCTA TTTTGTAAGG TACTGGATAA CAGAATTCTG GATCATGTTT 
AAGATGGATA CCAGCCTGGC AAGCACCATG GAGGAGTTTC AGGAACTGGT GAAAGCAAAT 
GGCGAGGAGC TCCACTGCCG CCTGATCGAC ACAACGCAAA TCAATGCCCG TGACTGGTCC 
AGGAAACTGA CCCAAAGGAT AAAATCGAAC ACCAGCAAGA AGCGGAAAGT CTCCCTGCTC 
TTTGACCATC TGGAGCCAGA AGAACTGTCT GAGCACCTGA CTTTCCTAGA ATTCAAGTCC 
TTCCGGAGGA TATCGTTCTC TGATTATCAG AATTACCTTG TGAACAGCTG CGTGAAGGAG 
AACCCCACCA TGGAGCGGTC CATTGCTCTG TGCAACGGCA TCTCCCAGTG GGTGCAGCTG 
ATGGTTCTCA GCCGCCCCAC CCCCCAGCTC CGAGCTGAAG TCTTCATCAA GTTCATCCAG 
GTGGCTCAGA AGCTCCATCA GCTGCAGAAC TTCAATACGT TGATGGCCGT GATCGGAGGG 
TTATGTCACA GCTCTATTTC CAGGCTCAAG GAGACAAGTT CACATGTCCC GCATGAAATC 
AATAAGGTTC TAGGTGAGAT GACTGAGCTG CTGTCCTCCT GTAGAAACTA CGACAACTAC 
CGGCGTGCTT ATGGGGAGTG CACCGACTTC AAGATCCCCA TCCTGGGGGT GCACCTCAAG 
GACCTCATCT CCCTGTACGA AGCCATGCCT GACTACCTGG AGGAGGGGAA GGTGAACGTC 
CATAAGCTGC TGGCCCTGTA CAACCACATC AATGAGTTAG TCCAGCTACA GGAGGTGCCC 
CCGCCCCTGG AGGCCAATAA GGACCTGGTG CACCTGCTGA CGTTATCCCT AGATCTCTAC 
TACACTGAAG ATGAAATCTA CGAACTTTCC TATGCCCGGG AACCCAGGAA TCACAAAGCC 
CCATCTGTCT TCAAGAACTA TGATCATGAC CAGGATGGAT ACATTTCCCA GGAAGAATTT 
GAAAAGATTG CTGCAAGTTT TCCATTTTCC TTCTGTGTGA TGGATAAAGA CAGGGAAGGC 
CTCATCAGCA GAGACGAGAT CACGGCCTAC TTCATGAGGG CCAGCTCCAT CTACTCCAAG 
TTGGGCCTCG GTTTTCCTCA CAACTTCCAA GAGACCACCT ACCTGAAGCC TACTTTCTGT 
GACAACTGTG CTGGATTTCT CTGGGGAGTG ATCAAACAAG GGTATCGGTG TAAAGACTGT 
GGGATGAACT GCCACAAACA ATGCAAAGAC CTGGTTGTGT TCGAATGCAA GAAGCGAGCC 
AAGAACTCAA CAGCTCCCAC AGAGATCAGC ACCTCTGTGG GGCCAACGTC CAACCTTTGC 
TCCTTGGGAG CCAAAGATCT GCTCCATGCA CCTGAGGAAG GACCTTTCAC ATTCCCTAAC 
GGGGAGGCTG CGGAACACAG TGAGGAGAGT AAGGATCGGA CCATCATGCT CATGGGAGTA 
TCCTCACAGA AGATTTCTGT TCGGCTGAAG AGGACTGTCG CCCACATGGC CACCCAGACT 
GAAGCACTGT CTTGGCCTGG CAGTGAGGGC CCTTCCAGTC ACTTTGTGCT GTCATCCCCA 
AGGAAGACAG CCCAGGATAC TCTGTATGTA CTGCCCAGCC CTACATCTCC GTGCCCCAGC 
CCAGTCTTGG TCAGGAAGCG GGCTTTCGTC AAGTGGGAGA ACAAAGAATC CTTCATAAAA 
TCAAAGGAGG AGCTCCGTCA CCTCAGGCTT CCAACATACC AAGAGCTGGA ACAGGAAATA 
AATACCCTGA AAGCAGATAA TAATGCTCTA AAGATCCAAC TGAAATATGC ACAGAAGAAG 
ATAGAAACCC TCCAACTTGC AAGAAACAAT CATGTCTTGG CTCAGATGGA GCAGGGTGAC 
TGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRP1 ( XM_015473118.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328604.1 Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa91667
Clone ID Related Accession (Same CDS sequence)	XM_015473118.1 , XM_024997639.1 , XM_015473118.2
Accession Version	XM_015473118.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	RAS guanyl-releasing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGC CTCTGGGACA TTTTGCCAAA GGAGCCAGCC TGGACGATCT CATCGACAGC 
TGTGTTCAAT CTTTTGATGC AGACGGAAAC CTGTGTCGAA GTAACCAACT GTTGCAAGTC 
ATGCTGACCA TGCACCGAAT TCTCATTTCC TCTGCTGAAC TGCTCCAAAA AGTTATCACT 
CTCTATAAGG ATGCCTTGGC AAAGAATTCA CCAGGGCTTT GCCTGAAGAT CTGCTATTTT 
GTAAGGTACT GGATAACAGA ATTCTGGATC ATGTTTAAGA TGGATACCAG CCTGGCAAGC 
ACCATGGAGG AGTTTCAGGA ACTGGTGAAA GCAAATGGCG AGGAGCTCCA CTGCCGCCTG 
ATCGACACAA CGCAAATCAA TGCCCGTGAC TGGTCCAGGA AACTGACCCA AAGGATAAAA 
TCGAACACCA GCAAGAAGCG GAAAGTCTCC CTGCTCTTTG ACCATCTGGA GCCAGAAGAA 
CTGTCTGAGC ACCTGACTTT CCTAGAATTC AAGTCCTTCC GGAGGATATC GTTCTCTGAT 
TATCAGAATT ACCTTGTGAA CAGCTGCGTG AAGGAGAACC CCACCATGGA GCGGTCCATT 
GCTCTGTGCA ACGGCATCTC CCAGTGGGTG CAGCTGATGG TTCTCAGCCG CCCCACCCCC 
CAGCTCCGAG CTGAAGTCTT CATCAAGTTC ATCCAGGTGG CTCAGAAGCT CCATCAGCTG 
CAGAACTTCA ATACGTTGAT GGCCGTGATC GGAGGGTTAT GTCACAGCTC TATTTCCAGG 
CTCAAGGAGA CAAGTTCACA TGTCCCGCAT GAAATCAATA AGGTTCTAGG TGAGATGACT 
GAGCTGCTGT CCTCCTGTAG AAACTACGAC AACTACCGGC GTGCTTATGG GGAGTGCACC 
GACTTCAAGA TCCCCATCCT GGGGGTGCAC CTCAAGGACC TCATCTCCCT GTACGAAGCC 
ATGCCTGACT ACCTGGAGGA GGGGAAGGTG AACGTCCATA AGCTGCTGGC CCTGTACAAC 
CACATCAATG AGTTAGTCCA GCTACAGGAG GTGCCCCCGC CCCTGGAGGC CAATAAGGAC 
CTGGTGCACC TGCTGACGTT ATCCCTAGAT CTCTACTACA CTGAAGATGA AATCTACGAA 
CTTTCCTATG CCCGGGAACC CAGGAATCAC AAAGCCCCAC CACTAACACC TTCAAAGCCA 
CCAGTAGTAG TGGACTGGGC CTCTGGAGTA TCTCCCAAGC CTGATCCAAA GACCATAAGC 
AAACACGTCC AGAGGATGGT GGATTCTGTC TTCAAGAACT ATGATCATGA CCAGGATGGA 
TACATTTCCC AGGAAGAATT TGAAAAGATT GCTGCAAGTT TTCCATTTTC CTTCTGTGTG 
ATGGATAAAG ACAGGGAAGG CCTCATCAGC AGAGACGAGA TCACGGCCTA CTTCATGAGG 
GCCAGCTCCA TCTACTCCAA GTTGGGCCTC GGTTTTCCTC ACAACTTCCA AGAGACCACC 
TACCTGAAGC CTACTTTCTG TGACAACTGT GCTGGATTTC TCTGGGGAGT GATCAAACAA 
GGGTATCGGT GTAAAGACTG TGGGATGAAC TGCCACAAAC AATGCAAAGA CCTGGTTGTG 
TTCGAATGCA AGAAGCGAGC CAAGAACTCA ACAGCTCCCA CAGAGATCAG CACCTCTGTG 
GGGCCAACGT CCAACCTTTG CTCCTTGGGA GCCAAAGATC TGCTCCATGC ACCTGAGGAA 
GGACCTTTCA CATTCCCTAA CGGGGAGGCT GCGGAACACA GTGAGGAGAG TAAGGATCGG 
ACCATCATGC TCATGGGAGT ATCCTCACAG AAGATTTCTG TTCGGCTGAA GAGGACTGTC 
GCCCACATGG CCACCCAGAC TGAAGCACTG TCTTGGCCTG GCAGTGAGGG CCCTTCCAGT 
CACTTTGTGC TGTCATCCCC AAGGAAGACA GCCCAGGATA CTCTGTATGT ACTGCCCAGC 
CCTACATCTC CGTGCCCCAG CCCAGTCTTG GTCAGGAAGC GGGCTTTCGT CAAGTGGGAG 
AACAAAGAAT CCTTCATAAA ATCAAAGGAG GAGCTCCGTC ACCTCAGGCT TCCAACATAC 
CAAGAGCTGG AACAGGAAAT AAATACCCTG AAAGCAGATA ATAATGCTCT AAAGATCCAA 
CTGAAATATG CACAGAAGAA GATAGAAACC CTCCAACTTG CAAGAAACAA TCATGTCTTG 
GCTCAGATGG AGCAGGGTGA CTGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328604.1
CDS	264..2513
Translation	MMVPLGHFAKGASLDDLIDSCVQSFDADGNLCRSNQLLQVMLTMHRILISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWIMFKMDTSLASTMEEFQELVKANGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTFLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEEGKVNVHKLLALYNHINELVQLQEVPPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHKAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNSTAPTEISTSVGPTSNLCSLGAKDLLHAPEEGPFTFPNGEAAEHSEESKDRTIMLMGVSSQKISVRLKRTVAHMATQTEALSWPGSEGPSSHFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKESFIKSKEELRHLRLPTYQELEQEINTLKADNNALKIQLKYAQKKIETLQLARNNHVLAQMEQGDCS

Target ORF information:

RefSeq Version	XM_015473118.1
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473118.1

ORF Insert Sequence:

ATGATGGTGC CTCTGGGACA TTTTGCCAAA GGAGCCAGCC TGGACGATCT CATCGACAGC 
TGTGTTCAAT CTTTTGATGC AGACGGAAAC CTGTGTCGAA GTAACCAACT GTTGCAAGTC 
ATGCTGACCA TGCACCGAAT TCTCATTTCC TCTGCTGAAC TGCTCCAAAA AGTTATCACT 
CTCTATAAGG ATGCCTTGGC AAAGAATTCA CCAGGGCTTT GCCTGAAGAT CTGCTATTTT 
GTAAGGTACT GGATAACAGA ATTCTGGATC ATGTTTAAGA TGGATACCAG CCTGGCAAGC 
ACCATGGAGG AGTTTCAGGA ACTGGTGAAA GCAAATGGCG AGGAGCTCCA CTGCCGCCTG 
ATCGACACAA CGCAAATCAA TGCCCGTGAC TGGTCCAGGA AACTGACCCA AAGGATAAAA 
TCGAACACCA GCAAGAAGCG GAAAGTCTCC CTGCTCTTTG ACCATCTGGA GCCAGAAGAA 
CTGTCTGAGC ACCTGACTTT CCTAGAATTC AAGTCCTTCC GGAGGATATC GTTCTCTGAT 
TATCAGAATT ACCTTGTGAA CAGCTGCGTG AAGGAGAACC CCACCATGGA GCGGTCCATT 
GCTCTGTGCA ACGGCATCTC CCAGTGGGTG CAGCTGATGG TTCTCAGCCG CCCCACCCCC 
CAGCTCCGAG CTGAAGTCTT CATCAAGTTC ATCCAGGTGG CTCAGAAGCT CCATCAGCTG 
CAGAACTTCA ATACGTTGAT GGCCGTGATC GGAGGGTTAT GTCACAGCTC TATTTCCAGG 
CTCAAGGAGA CAAGTTCACA TGTCCCGCAT GAAATCAATA AGGTTCTAGG TGAGATGACT 
GAGCTGCTGT CCTCCTGTAG AAACTACGAC AACTACCGGC GTGCTTATGG GGAGTGCACC 
GACTTCAAGA TCCCCATCCT GGGGGTGCAC CTCAAGGACC TCATCTCCCT GTACGAAGCC 
ATGCCTGACT ACCTGGAGGA GGGGAAGGTG AACGTCCATA AGCTGCTGGC CCTGTACAAC 
CACATCAATG AGTTAGTCCA GCTACAGGAG GTGCCCCCGC CCCTGGAGGC CAATAAGGAC 
CTGGTGCACC TGCTGACGTT ATCCCTAGAT CTCTACTACA CTGAAGATGA AATCTACGAA 
CTTTCCTATG CCCGGGAACC CAGGAATCAC AAAGCCCCAC CACTAACACC TTCAAAGCCA 
CCAGTAGTAG TGGACTGGGC CTCTGGAGTA TCTCCCAAGC CTGATCCAAA GACCATAAGC 
AAACACGTCC AGAGGATGGT GGATTCTGTC TTCAAGAACT ATGATCATGA CCAGGATGGA 
TACATTTCCC AGGAAGAATT TGAAAAGATT GCTGCAAGTT TTCCATTTTC CTTCTGTGTG 
ATGGATAAAG ACAGGGAAGG CCTCATCAGC AGAGACGAGA TCACGGCCTA CTTCATGAGG 
GCCAGCTCCA TCTACTCCAA GTTGGGCCTC GGTTTTCCTC ACAACTTCCA AGAGACCACC 
TACCTGAAGC CTACTTTCTG TGACAACTGT GCTGGATTTC TCTGGGGAGT GATCAAACAA 
GGGTATCGGT GTAAAGACTG TGGGATGAAC TGCCACAAAC AATGCAAAGA CCTGGTTGTG 
TTCGAATGCA AGAAGCGAGC CAAGAACTCA ACAGCTCCCA CAGAGATCAG CACCTCTGTG 
GGGCCAACGT CCAACCTTTG CTCCTTGGGA GCCAAAGATC TGCTCCATGC ACCTGAGGAA 
GGACCTTTCA CATTCCCTAA CGGGGAGGCT GCGGAACACA GTGAGGAGAG TAAGGATCGG 
ACCATCATGC TCATGGGAGT ATCCTCACAG AAGATTTCTG TTCGGCTGAA GAGGACTGTC 
GCCCACATGG CCACCCAGAC TGAAGCACTG TCTTGGCCTG GCAGTGAGGG CCCTTCCAGT 
CACTTTGTGC TGTCATCCCC AAGGAAGACA GCCCAGGATA CTCTGTATGT ACTGCCCAGC 
CCTACATCTC CGTGCCCCAG CCCAGTCTTG GTCAGGAAGC GGGCTTTCGT CAAGTGGGAG 
AACAAAGAAT CCTTCATAAA ATCAAAGGAG GAGCTCCGTC ACCTCAGGCT TCCAACATAC 
CAAGAGCTGG AACAGGAAAT AAATACCCTG AAAGCAGATA ATAATGCTCT AAAGATCCAA 
CTGAAATATG CACAGAAGAA GATAGAAACC CTCCAACTTG CAAGAAACAA TCATGTCTTG 
GCTCAGATGG AGCAGGGTGA CTGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRP1 ( XM_024997639.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853407.1 Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa91667
Clone ID Related Accession (Same CDS sequence)	XM_015473118.1 , XM_024997639.1 , XM_015473118.2
Accession Version	XM_024997639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RAS guanyl-releasing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGC CTCTGGGACA TTTTGCCAAA GGAGCCAGCC TGGACGATCT CATCGACAGC 
TGTGTTCAAT CTTTTGATGC AGACGGAAAC CTGTGTCGAA GTAACCAACT GTTGCAAGTC 
ATGCTGACCA TGCACCGAAT TCTCATTTCC TCTGCTGAAC TGCTCCAAAA AGTTATCACT 
CTCTATAAGG ATGCCTTGGC AAAGAATTCA CCAGGGCTTT GCCTGAAGAT CTGCTATTTT 
GTAAGGTACT GGATAACAGA ATTCTGGATC ATGTTTAAGA TGGATACCAG CCTGGCAAGC 
ACCATGGAGG AGTTTCAGGA ACTGGTGAAA GCAAATGGCG AGGAGCTCCA CTGCCGCCTG 
ATCGACACAA CGCAAATCAA TGCCCGTGAC TGGTCCAGGA AACTGACCCA AAGGATAAAA 
TCGAACACCA GCAAGAAGCG GAAAGTCTCC CTGCTCTTTG ACCATCTGGA GCCAGAAGAA 
CTGTCTGAGC ACCTGACTTT CCTAGAATTC AAGTCCTTCC GGAGGATATC GTTCTCTGAT 
TATCAGAATT ACCTTGTGAA CAGCTGCGTG AAGGAGAACC CCACCATGGA GCGGTCCATT 
GCTCTGTGCA ACGGCATCTC CCAGTGGGTG CAGCTGATGG TTCTCAGCCG CCCCACCCCC 
CAGCTCCGAG CTGAAGTCTT CATCAAGTTC ATCCAGGTGG CTCAGAAGCT CCATCAGCTG 
CAGAACTTCA ATACGTTGAT GGCCGTGATC GGAGGGTTAT GTCACAGCTC TATTTCCAGG 
CTCAAGGAGA CAAGTTCACA TGTCCCGCAT GAAATCAATA AGGTTCTAGG TGAGATGACT 
GAGCTGCTGT CCTCCTGTAG AAACTACGAC AACTACCGGC GTGCTTATGG GGAGTGCACC 
GACTTCAAGA TCCCCATCCT GGGGGTGCAC CTCAAGGACC TCATCTCCCT GTACGAAGCC 
ATGCCTGACT ACCTGGAGGA GGGGAAGGTG AACGTCCATA AGCTGCTGGC CCTGTACAAC 
CACATCAATG AGTTAGTCCA GCTACAGGAG GTGCCCCCGC CCCTGGAGGC CAATAAGGAC 
CTGGTGCACC TGCTGACGTT ATCCCTAGAT CTCTACTACA CTGAAGATGA AATCTACGAA 
CTTTCCTATG CCCGGGAACC CAGGAATCAC AAAGCCCCAC CACTAACACC TTCAAAGCCA 
CCAGTAGTAG TGGACTGGGC CTCTGGAGTA TCTCCCAAGC CTGATCCAAA GACCATAAGC 
AAACACGTCC AGAGGATGGT GGATTCTGTC TTCAAGAACT ATGATCATGA CCAGGATGGA 
TACATTTCCC AGGAAGAATT TGAAAAGATT GCTGCAAGTT TTCCATTTTC CTTCTGTGTG 
ATGGATAAAG ACAGGGAAGG CCTCATCAGC AGAGACGAGA TCACGGCCTA CTTCATGAGG 
GCCAGCTCCA TCTACTCCAA GTTGGGCCTC GGTTTTCCTC ACAACTTCCA AGAGACCACC 
TACCTGAAGC CTACTTTCTG TGACAACTGT GCTGGATTTC TCTGGGGAGT GATCAAACAA 
GGGTATCGGT GTAAAGACTG TGGGATGAAC TGCCACAAAC AATGCAAAGA CCTGGTTGTG 
TTCGAATGCA AGAAGCGAGC CAAGAACTCA ACAGCTCCCA CAGAGATCAG CACCTCTGTG 
GGGCCAACGT CCAACCTTTG CTCCTTGGGA GCCAAAGATC TGCTCCATGC ACCTGAGGAA 
GGACCTTTCA CATTCCCTAA CGGGGAGGCT GCGGAACACA GTGAGGAGAG TAAGGATCGG 
ACCATCATGC TCATGGGAGT ATCCTCACAG AAGATTTCTG TTCGGCTGAA GAGGACTGTC 
GCCCACATGG CCACCCAGAC TGAAGCACTG TCTTGGCCTG GCAGTGAGGG CCCTTCCAGT 
CACTTTGTGC TGTCATCCCC AAGGAAGACA GCCCAGGATA CTCTGTATGT ACTGCCCAGC 
CCTACATCTC CGTGCCCCAG CCCAGTCTTG GTCAGGAAGC GGGCTTTCGT CAAGTGGGAG 
AACAAAGAAT CCTTCATAAA ATCAAAGGAG GAGCTCCGTC ACCTCAGGCT TCCAACATAC 
CAAGAGCTGG AACAGGAAAT AAATACCCTG AAAGCAGATA ATAATGCTCT AAAGATCCAA 
CTGAAATATG CACAGAAGAA GATAGAAACC CTCCAACTTG CAAGAAACAA TCATGTCTTG 
GCTCAGATGG AGCAGGGTGA CTGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853407.1
CDS	492..2741
Translation	MMVPLGHFAKGASLDDLIDSCVQSFDADGNLCRSNQLLQVMLTMHRILISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWIMFKMDTSLASTMEEFQELVKANGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTFLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEEGKVNVHKLLALYNHINELVQLQEVPPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHKAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNSTAPTEISTSVGPTSNLCSLGAKDLLHAPEEGPFTFPNGEAAEHSEESKDRTIMLMGVSSQKISVRLKRTVAHMATQTEALSWPGSEGPSSHFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKESFIKSKEELRHLRLPTYQELEQEINTLKADNNALKIQLKYAQKKIETLQLARNNHVLAQMEQGDCS

Target ORF information:

RefSeq Version	XM_024997639.1
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997639.1

ORF Insert Sequence:

ATGATGGTGC CTCTGGGACA TTTTGCCAAA GGAGCCAGCC TGGACGATCT CATCGACAGC 
TGTGTTCAAT CTTTTGATGC AGACGGAAAC CTGTGTCGAA GTAACCAACT GTTGCAAGTC 
ATGCTGACCA TGCACCGAAT TCTCATTTCC TCTGCTGAAC TGCTCCAAAA AGTTATCACT 
CTCTATAAGG ATGCCTTGGC AAAGAATTCA CCAGGGCTTT GCCTGAAGAT CTGCTATTTT 
GTAAGGTACT GGATAACAGA ATTCTGGATC ATGTTTAAGA TGGATACCAG CCTGGCAAGC 
ACCATGGAGG AGTTTCAGGA ACTGGTGAAA GCAAATGGCG AGGAGCTCCA CTGCCGCCTG 
ATCGACACAA CGCAAATCAA TGCCCGTGAC TGGTCCAGGA AACTGACCCA AAGGATAAAA 
TCGAACACCA GCAAGAAGCG GAAAGTCTCC CTGCTCTTTG ACCATCTGGA GCCAGAAGAA 
CTGTCTGAGC ACCTGACTTT CCTAGAATTC AAGTCCTTCC GGAGGATATC GTTCTCTGAT 
TATCAGAATT ACCTTGTGAA CAGCTGCGTG AAGGAGAACC CCACCATGGA GCGGTCCATT 
GCTCTGTGCA ACGGCATCTC CCAGTGGGTG CAGCTGATGG TTCTCAGCCG CCCCACCCCC 
CAGCTCCGAG CTGAAGTCTT CATCAAGTTC ATCCAGGTGG CTCAGAAGCT CCATCAGCTG 
CAGAACTTCA ATACGTTGAT GGCCGTGATC GGAGGGTTAT GTCACAGCTC TATTTCCAGG 
CTCAAGGAGA CAAGTTCACA TGTCCCGCAT GAAATCAATA AGGTTCTAGG TGAGATGACT 
GAGCTGCTGT CCTCCTGTAG AAACTACGAC AACTACCGGC GTGCTTATGG GGAGTGCACC 
GACTTCAAGA TCCCCATCCT GGGGGTGCAC CTCAAGGACC TCATCTCCCT GTACGAAGCC 
ATGCCTGACT ACCTGGAGGA GGGGAAGGTG AACGTCCATA AGCTGCTGGC CCTGTACAAC 
CACATCAATG AGTTAGTCCA GCTACAGGAG GTGCCCCCGC CCCTGGAGGC CAATAAGGAC 
CTGGTGCACC TGCTGACGTT ATCCCTAGAT CTCTACTACA CTGAAGATGA AATCTACGAA 
CTTTCCTATG CCCGGGAACC CAGGAATCAC AAAGCCCCAC CACTAACACC TTCAAAGCCA 
CCAGTAGTAG TGGACTGGGC CTCTGGAGTA TCTCCCAAGC CTGATCCAAA GACCATAAGC 
AAACACGTCC AGAGGATGGT GGATTCTGTC TTCAAGAACT ATGATCATGA CCAGGATGGA 
TACATTTCCC AGGAAGAATT TGAAAAGATT GCTGCAAGTT TTCCATTTTC CTTCTGTGTG 
ATGGATAAAG ACAGGGAAGG CCTCATCAGC AGAGACGAGA TCACGGCCTA CTTCATGAGG 
GCCAGCTCCA TCTACTCCAA GTTGGGCCTC GGTTTTCCTC ACAACTTCCA AGAGACCACC 
TACCTGAAGC CTACTTTCTG TGACAACTGT GCTGGATTTC TCTGGGGAGT GATCAAACAA 
GGGTATCGGT GTAAAGACTG TGGGATGAAC TGCCACAAAC AATGCAAAGA CCTGGTTGTG 
TTCGAATGCA AGAAGCGAGC CAAGAACTCA ACAGCTCCCA CAGAGATCAG CACCTCTGTG 
GGGCCAACGT CCAACCTTTG CTCCTTGGGA GCCAAAGATC TGCTCCATGC ACCTGAGGAA 
GGACCTTTCA CATTCCCTAA CGGGGAGGCT GCGGAACACA GTGAGGAGAG TAAGGATCGG 
ACCATCATGC TCATGGGAGT ATCCTCACAG AAGATTTCTG TTCGGCTGAA GAGGACTGTC 
GCCCACATGG CCACCCAGAC TGAAGCACTG TCTTGGCCTG GCAGTGAGGG CCCTTCCAGT 
CACTTTGTGC TGTCATCCCC AAGGAAGACA GCCCAGGATA CTCTGTATGT ACTGCCCAGC 
CCTACATCTC CGTGCCCCAG CCCAGTCTTG GTCAGGAAGC GGGCTTTCGT CAAGTGGGAG 
AACAAAGAAT CCTTCATAAA ATCAAAGGAG GAGCTCCGTC ACCTCAGGCT TCCAACATAC 
CAAGAGCTGG AACAGGAAAT AAATACCCTG AAAGCAGATA ATAATGCTCT AAAGATCCAA 
CTGAAATATG CACAGAAGAA GATAGAAACC CTCCAACTTG CAAGAAACAA TCATGTCTTG 
GCTCAGATGG AGCAGGGTGA CTGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRP1 ( XM_015473118.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328604.1 Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X3, mRNA.

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CloneID	OBa91667
Clone ID Related Accession (Same CDS sequence)	XM_015473118.1 , XM_024997639.1 , XM_015473118.2
Accession Version	XM_015473118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RAS guanyl-releasing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGC CTCTGGGACA TTTTGCCAAA GGAGCCAGCC TGGACGATCT CATCGACAGC 
TGTGTTCAAT CTTTTGATGC AGACGGAAAC CTGTGTCGAA GTAACCAACT GTTGCAAGTC 
ATGCTGACCA TGCACCGAAT TCTCATTTCC TCTGCTGAAC TGCTCCAAAA AGTTATCACT 
CTCTATAAGG ATGCCTTGGC AAAGAATTCA CCAGGGCTTT GCCTGAAGAT CTGCTATTTT 
GTAAGGTACT GGATAACAGA ATTCTGGATC ATGTTTAAGA TGGATACCAG CCTGGCAAGC 
ACCATGGAGG AGTTTCAGGA ACTGGTGAAA GCAAATGGCG AGGAGCTCCA CTGCCGCCTG 
ATCGACACAA CGCAAATCAA TGCCCGTGAC TGGTCCAGGA AACTGACCCA AAGGATAAAA 
TCGAACACCA GCAAGAAGCG GAAAGTCTCC CTGCTCTTTG ACCATCTGGA GCCAGAAGAA 
CTGTCTGAGC ACCTGACTTT CCTAGAATTC AAGTCCTTCC GGAGGATATC GTTCTCTGAT 
TATCAGAATT ACCTTGTGAA CAGCTGCGTG AAGGAGAACC CCACCATGGA GCGGTCCATT 
GCTCTGTGCA ACGGCATCTC CCAGTGGGTG CAGCTGATGG TTCTCAGCCG CCCCACCCCC 
CAGCTCCGAG CTGAAGTCTT CATCAAGTTC ATCCAGGTGG CTCAGAAGCT CCATCAGCTG 
CAGAACTTCA ATACGTTGAT GGCCGTGATC GGAGGGTTAT GTCACAGCTC TATTTCCAGG 
CTCAAGGAGA CAAGTTCACA TGTCCCGCAT GAAATCAATA AGGTTCTAGG TGAGATGACT 
GAGCTGCTGT CCTCCTGTAG AAACTACGAC AACTACCGGC GTGCTTATGG GGAGTGCACC 
GACTTCAAGA TCCCCATCCT GGGGGTGCAC CTCAAGGACC TCATCTCCCT GTACGAAGCC 
ATGCCTGACT ACCTGGAGGA GGGGAAGGTG AACGTCCATA AGCTGCTGGC CCTGTACAAC 
CACATCAATG AGTTAGTCCA GCTACAGGAG GTGCCCCCGC CCCTGGAGGC CAATAAGGAC 
CTGGTGCACC TGCTGACGTT ATCCCTAGAT CTCTACTACA CTGAAGATGA AATCTACGAA 
CTTTCCTATG CCCGGGAACC CAGGAATCAC AAAGCCCCAC CACTAACACC TTCAAAGCCA 
CCAGTAGTAG TGGACTGGGC CTCTGGAGTA TCTCCCAAGC CTGATCCAAA GACCATAAGC 
AAACACGTCC AGAGGATGGT GGATTCTGTC TTCAAGAACT ATGATCATGA CCAGGATGGA 
TACATTTCCC AGGAAGAATT TGAAAAGATT GCTGCAAGTT TTCCATTTTC CTTCTGTGTG 
ATGGATAAAG ACAGGGAAGG CCTCATCAGC AGAGACGAGA TCACGGCCTA CTTCATGAGG 
GCCAGCTCCA TCTACTCCAA GTTGGGCCTC GGTTTTCCTC ACAACTTCCA AGAGACCACC 
TACCTGAAGC CTACTTTCTG TGACAACTGT GCTGGATTTC TCTGGGGAGT GATCAAACAA 
GGGTATCGGT GTAAAGACTG TGGGATGAAC TGCCACAAAC AATGCAAAGA CCTGGTTGTG 
TTCGAATGCA AGAAGCGAGC CAAGAACTCA ACAGCTCCCA CAGAGATCAG CACCTCTGTG 
GGGCCAACGT CCAACCTTTG CTCCTTGGGA GCCAAAGATC TGCTCCATGC ACCTGAGGAA 
GGACCTTTCA CATTCCCTAA CGGGGAGGCT GCGGAACACA GTGAGGAGAG TAAGGATCGG 
ACCATCATGC TCATGGGAGT ATCCTCACAG AAGATTTCTG TTCGGCTGAA GAGGACTGTC 
GCCCACATGG CCACCCAGAC TGAAGCACTG TCTTGGCCTG GCAGTGAGGG CCCTTCCAGT 
CACTTTGTGC TGTCATCCCC AAGGAAGACA GCCCAGGATA CTCTGTATGT ACTGCCCAGC 
CCTACATCTC CGTGCCCCAG CCCAGTCTTG GTCAGGAAGC GGGCTTTCGT CAAGTGGGAG 
AACAAAGAAT CCTTCATAAA ATCAAAGGAG GAGCTCCGTC ACCTCAGGCT TCCAACATAC 
CAAGAGCTGG AACAGGAAAT AAATACCCTG AAAGCAGATA ATAATGCTCT AAAGATCCAA 
CTGAAATATG CACAGAAGAA GATAGAAACC CTCCAACTTG CAAGAAACAA TCATGTCTTG 
GCTCAGATGG AGCAGGGTGA CTGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328604.1
CDS	261..2510
Translation	MMVPLGHFAKGASLDDLIDSCVQSFDADGNLCRSNQLLQVMLTMHRILISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWIMFKMDTSLASTMEEFQELVKANGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELSEHLTFLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQVAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTDFKIPILGVHLKDLISLYEAMPDYLEEGKVNVHKLLALYNHINELVQLQEVPPPLEANKDLVHLLTLSLDLYYTEDEIYELSYAREPRNHKAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYISQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAGFLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNSTAPTEISTSVGPTSNLCSLGAKDLLHAPEEGPFTFPNGEAAEHSEESKDRTIMLMGVSSQKISVRLKRTVAHMATQTEALSWPGSEGPSSHFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKESFIKSKEELRHLRLPTYQELEQEINTLKADNNALKIQLKYAQKKIETLQLARNNHVLAQMEQGDCS

Target ORF information:

RefSeq Version	XM_015473118.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS guanyl releasing protein 1 (RASGRP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473118.2

ORF Insert Sequence:

ATGATGGTGC CTCTGGGACA TTTTGCCAAA GGAGCCAGCC TGGACGATCT CATCGACAGC 
TGTGTTCAAT CTTTTGATGC AGACGGAAAC CTGTGTCGAA GTAACCAACT GTTGCAAGTC 
ATGCTGACCA TGCACCGAAT TCTCATTTCC TCTGCTGAAC TGCTCCAAAA AGTTATCACT 
CTCTATAAGG ATGCCTTGGC AAAGAATTCA CCAGGGCTTT GCCTGAAGAT CTGCTATTTT 
GTAAGGTACT GGATAACAGA ATTCTGGATC ATGTTTAAGA TGGATACCAG CCTGGCAAGC 
ACCATGGAGG AGTTTCAGGA ACTGGTGAAA GCAAATGGCG AGGAGCTCCA CTGCCGCCTG 
ATCGACACAA CGCAAATCAA TGCCCGTGAC TGGTCCAGGA AACTGACCCA AAGGATAAAA 
TCGAACACCA GCAAGAAGCG GAAAGTCTCC CTGCTCTTTG ACCATCTGGA GCCAGAAGAA 
CTGTCTGAGC ACCTGACTTT CCTAGAATTC AAGTCCTTCC GGAGGATATC GTTCTCTGAT 
TATCAGAATT ACCTTGTGAA CAGCTGCGTG AAGGAGAACC CCACCATGGA GCGGTCCATT 
GCTCTGTGCA ACGGCATCTC CCAGTGGGTG CAGCTGATGG TTCTCAGCCG CCCCACCCCC 
CAGCTCCGAG CTGAAGTCTT CATCAAGTTC ATCCAGGTGG CTCAGAAGCT CCATCAGCTG 
CAGAACTTCA ATACGTTGAT GGCCGTGATC GGAGGGTTAT GTCACAGCTC TATTTCCAGG 
CTCAAGGAGA CAAGTTCACA TGTCCCGCAT GAAATCAATA AGGTTCTAGG TGAGATGACT 
GAGCTGCTGT CCTCCTGTAG AAACTACGAC AACTACCGGC GTGCTTATGG GGAGTGCACC 
GACTTCAAGA TCCCCATCCT GGGGGTGCAC CTCAAGGACC TCATCTCCCT GTACGAAGCC 
ATGCCTGACT ACCTGGAGGA GGGGAAGGTG AACGTCCATA AGCTGCTGGC CCTGTACAAC 
CACATCAATG AGTTAGTCCA GCTACAGGAG GTGCCCCCGC CCCTGGAGGC CAATAAGGAC 
CTGGTGCACC TGCTGACGTT ATCCCTAGAT CTCTACTACA CTGAAGATGA AATCTACGAA 
CTTTCCTATG CCCGGGAACC CAGGAATCAC AAAGCCCCAC CACTAACACC TTCAAAGCCA 
CCAGTAGTAG TGGACTGGGC CTCTGGAGTA TCTCCCAAGC CTGATCCAAA GACCATAAGC 
AAACACGTCC AGAGGATGGT GGATTCTGTC TTCAAGAACT ATGATCATGA CCAGGATGGA 
TACATTTCCC AGGAAGAATT TGAAAAGATT GCTGCAAGTT TTCCATTTTC CTTCTGTGTG 
ATGGATAAAG ACAGGGAAGG CCTCATCAGC AGAGACGAGA TCACGGCCTA CTTCATGAGG 
GCCAGCTCCA TCTACTCCAA GTTGGGCCTC GGTTTTCCTC ACAACTTCCA AGAGACCACC 
TACCTGAAGC CTACTTTCTG TGACAACTGT GCTGGATTTC TCTGGGGAGT GATCAAACAA 
GGGTATCGGT GTAAAGACTG TGGGATGAAC TGCCACAAAC AATGCAAAGA CCTGGTTGTG 
TTCGAATGCA AGAAGCGAGC CAAGAACTCA ACAGCTCCCA CAGAGATCAG CACCTCTGTG 
GGGCCAACGT CCAACCTTTG CTCCTTGGGA GCCAAAGATC TGCTCCATGC ACCTGAGGAA 
GGACCTTTCA CATTCCCTAA CGGGGAGGCT GCGGAACACA GTGAGGAGAG TAAGGATCGG 
ACCATCATGC TCATGGGAGT ATCCTCACAG AAGATTTCTG TTCGGCTGAA GAGGACTGTC 
GCCCACATGG CCACCCAGAC TGAAGCACTG TCTTGGCCTG GCAGTGAGGG CCCTTCCAGT 
CACTTTGTGC TGTCATCCCC AAGGAAGACA GCCCAGGATA CTCTGTATGT ACTGCCCAGC 
CCTACATCTC CGTGCCCCAG CCCAGTCTTG GTCAGGAAGC GGGCTTTCGT CAAGTGGGAG 
AACAAAGAAT CCTTCATAAA ATCAAAGGAG GAGCTCCGTC ACCTCAGGCT TCCAACATAC 
CAAGAGCTGG AACAGGAAAT AAATACCCTG AAAGCAGATA ATAATGCTCT AAAGATCCAA 
CTGAAATATG CACAGAAGAA GATAGAAACC CTCCAACTTG CAAGAAACAA TCATGTCTTG 
GCTCAGATGG AGCAGGGTGA CTGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Segregation of the hereditary thrombopathia-associated polymorphism in polled German Fleckvieh cattle.
Animal genetics46(5)584-5(2015 Oct)
Zerbin I,Metzger J,Dierks C,Distl O

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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RASGRP1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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