SPHK2 cDNA ORF clone, Bos taurus(cattle)

The following SPHK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225686 XM_024979237.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa225687 XM_024979240.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa225687 XM_024979241.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa225687 XM_024979239.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa225687 XM_024979238.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa225682 XM_024979227.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225682 XM_024979228.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225683 XM_024979230.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225683 XM_015458245.2
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225683 XM_024979232.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225683 XM_024979229.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225683 XM_024979231.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225684 XM_024979233.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225685 XM_024979236.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225685 XM_024979235.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa225685 XM_024979234.1
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa94996 XM_005195452.2
Latest version!
Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225686
    Clone ID Related Accession (Same CDS sequence) XM_024979237.1
    Accession Version XM_024979237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCACAAG GAGAAATCAC CCCGGACCTC CTGCCTAGGC CGCCCCGATT GCTCCTATTG 
    GTCAATCCCT TTGGGGGGCG GGGCCTGGCC TGGCAGTGGT GTAAGAACCA TGTGCTGCCC
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835005.1
    CDS393..1859
    Translation

    Target ORF information:

    RefSeq Version XM_024979237.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCACAAG GAGAAATCAC CCCGGACCTC CTGCCTAGGC CGCCCCGATT GCTCCTATTG 
    GTCAATCCCT TTGGGGGGCG GGGCCTGGCC TGGCAGTGGT GTAAGAACCA TGTGCTGCCC
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225687
    Clone ID Related Accession (Same CDS sequence) XM_024979241.1 , XM_024979238.1 , XM_024979239.1 , XM_024979240.1
    Accession Version XM_024979241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835009.1
    CDS344..1690
    Translation

    Target ORF information:

    RefSeq Version XM_024979241.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225687
    Clone ID Related Accession (Same CDS sequence) XM_024979241.1 , XM_024979238.1 , XM_024979239.1 , XM_024979240.1
    Accession Version XM_024979238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835006.1
    CDS531..1877
    Translation

    Target ORF information:

    RefSeq Version XM_024979238.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225687
    Clone ID Related Accession (Same CDS sequence) XM_024979241.1 , XM_024979238.1 , XM_024979239.1 , XM_024979240.1
    Accession Version XM_024979239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835007.1
    CDS535..1881
    Translation

    Target ORF information:

    RefSeq Version XM_024979239.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225687
    Clone ID Related Accession (Same CDS sequence) XM_024979241.1 , XM_024979238.1 , XM_024979239.1 , XM_024979240.1
    Accession Version XM_024979240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835008.1
    CDS509..1855
    Translation

    Target ORF information:

    RefSeq Version XM_024979240.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCCG AAGCGGGGCT GTCCTTCAAC CTGATCCAGA CAGAACGACA GAACCATGCC 
    CGTGAGCTGG TCCAGGGGCT GAACCTGAGC GAATGGGACG GCATCGTCAC AGTCTCGGGA
    GATGGGCTGC TGTATGAGGT GCTGAATGGA CTCCTACAAC GCCCCGACTG GGAAGAGGCT
    GTGAAGACCC CCGTGGGCAT TCTCCCCTGT GGCTCGGGCA ATGCGTTAGC AGGAGCCGTG
    AACCGGCATG GGGGATTTGA GCCTGCCCTG GGCATTGACC TGCTACTCAA CTGCTCCTTA
    CTGCTCTGCC GGGGTGGCAG CCACCCGCTG GACCTGCTCT CCGTGACTCT GGCCTCCGGC
    TCCCGCTGTT TCTCTTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGACATCCAG
    AGCGAGCGCT TCAGGGCCCT GGGCAGTGCT CGCTTCACGC TGGGCACAGT CCTGGGCCTT
    GCCACGCTGC ACACCTACCG CGGCCGCTTC TCCTACCTCC CTGCCACTGT GGAACCCGCC
    TCGCCTGCCC CTGCCCATGG CCTGCCAAGG GCCAAGTCAG AGTTGACCCT GGCCCCGGCC
    CCAGCCCCAC CCGTGGCCCA CTCACCCCTG CATCGCTCAG TGTCAGACCT GCCCCTGCCC
    CTGCCTCAGC CTGCCCTGAC CTCCCCTGGC TCACCTGAAC CCCTGCCCAT CCTGTCTCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC CCCACTCTCC
    CCGGACCCAC TGCTGCCCTC GCCCCCGGGG ACCCCCAAGG CAGCTCAGCT CTCACCCATC
    AGCGAGGGGG CCTCAGAAAT GGCAGCATCC TCTGGGCTCC CACCTCCCAC CCCTGGTGCC
    CCAGTATCCA TCTCTGCTGG GGGACCACCT GATCACCTGC TCCCTCCTCT AGGCACTCCG
    ATACCCCCAG GCTGGGTGAC GATGGAGGGA GACTTTGTGC TTATTTTGGC CATCTTACCC
    AGCCACCTGG GGGCTGACCT CGTGGCGGCC CCCCACGCGC GCTTTGACGA CGGCCTGGTG
    CACCTGTGCT GGGTTCGCAG CGGCATCTCG CGGGCGGCGC TACTGCGCAT GTTTCTGGCC
    ATGGAGCGTG GTACCCACTT CAGCCTGGGC TGTCCGTACC TGGGCTACGC CGCAGCTCAT
    GCCTTCCGCC TAGAGCCGCT CACGCCCCGC GGCGTGCTCA CGGTGGACGG GGAGCAGGTG
    GAGTACGGGC CATTGCAGGC GCAGGTGCAT CCTGGCCTCG GTACATTGCT CACGGGTCCT
    CCAGGCTGCC CTGGGCGGGA AGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225682
    Clone ID Related Accession (Same CDS sequence) XM_024979227.1 , XM_024979228.1
    Accession Version XM_024979227.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTGGCTCT CCCTCTCCAA CCCCTTAGTT CCCCCATGTC ACACCCTTTC TCAGTCTTTC 
    CAGAGGCCAG AGCAGGAGCT GACCTGGAGC TGGGGCTACC GGCCTAGAAG CGCCCTGGAC
    AGGGTCAAGG CCATGGCCCC CCCACCGCCA CTGGCCCCCA GCACCCCACT CCTGCATGGC
    GAGTTTGGCT CCTACCCAGA CCGCGGCCCA CGCTTCGCCC TCACTCTCAC ACCACAAGCC
    CTGCACATAC AGCGGTTGCG CCCAAAGCCC GAGGCCCGGC CCCGGGGTGG CCTGGTCCTG
    CTGACCGAGG TCTCAGGCTG CTGCACCCTG CGGAGCCGAA GCCCCCTGGA CTCAGCAGCC
    TACTTCTGCG TCTACACCTA CCCCAGGGGC CGGCGCGGGG CCCGGCGCAG AGCTGCACGC
    ACCTTCCGGG CCGACGGGGC AGCCACCTAC GAGGAGAACC GCGCTGAGGC CCAGCGCTGG
    GCCACTGCCC TCACGTGTCT GCTCCGTGGA CTGCCACTGC CTGGGGACGG GGAAATCACC
    CCGGACCTCC TGCCTAGGCC GCCCCGATTG CTCCTATTGG TCAATCCCTT TGGGGGGCGG
    GGCCTGGCCT GGCAGTGGTG TAAGAACCAT GTGCTGCCCA TGATCTCCGA AGCGGGGCTG
    TCCTTCAACC TGATCCAGAC AGAACGACAG AACCATGCCC GTGAGCTGGT CCAGGGGCTG
    AACCTGAGCG AATGGGACGG CATCGTCACA GTCTCGGGAG ATGGGCTGCT GTATGAGGTG
    CTGAATGGAC TCCTACAACG CCCCGACTGG GAAGAGGCTG TGAAGACCCC CGTGGGCATT
    CTCCCCTGTG GCTCGGGCAA TGCGTTAGCA GGAGCCGTGA ACCGGCATGG GGGATTTGAG
    CCTGCCCTGG GCATTGACCT GCTACTCAAC TGCTCCTTAC TGCTCTGCCG GGGTGGCAGC
    CACCCGCTGG ACCTGCTCTC CGTGACTCTG GCCTCCGGCT CCCGCTGTTT CTCTTTCCTG
    TCTGTGGCCT GGGGCTTCGT GTCAGATGTG GACATCCAGA GCGAGCGCTT CAGGGCCCTG
    GGCAGTGCTC GCTTCACGCT GGGCACAGTC CTGGGCCTTG CCACGCTGCA CACCTACCGC
    GGCCGCTTCT CCTACCTCCC TGCCACTGTG GAACCCGCCT CGCCTGCCCC TGCCCATGGC
    CTGCCAAGGG CCAAGTCAGA GTTGACCCTG GCCCCGGCCC CAGCCCCACC CGTGGCCCAC
    TCACCCCTGC ATCGCTCAGT GTCAGACCTG CCCCTGCCCC TGCCTCAGCC TGCCCTGACC
    TCCCCTGGCT CACCTGAACC CCTGCCCATC CTGTCTCTCA ACGGTGGGGG CCCAGAGCTG
    GCTGGGGACT GGGGTGGGGC TGGGGATGCC CCACTCTCCC CGGACCCACT GCTGCCCTCG
    CCCCCGGGGA CCCCCAAGGC AGCTCAGCTC TCACCCATCA GCGAGGGGGC CTCAGAAATG
    GCAGCATCCT CTGGGCTCCC ACCTCCCACC CCTGGTGCCC CAGTATCCAT CTCTGCTGGG
    GGACCACCTG ATCACCTGCT CCCTCCTCTA GGCACTCCGA TACCCCCAGG CTGGGTGACG
    ATGGAGGGAG ACTTTGTGCT TATTTTGGCC ATCTTACCCA GCCACCTGGG GGCTGACCTC
    GTGGCGGCCC CCCACGCGCG CTTTGACGAC GGCCTGGTGC ACCTGTGCTG GGTTCGCAGC
    GGCATCTCGC GGGCGGCGCT ACTGCGCATG TTTCTGGCCA TGGAGCGTGG TACCCACTTC
    AGCCTGGGCT GTCCGTACCT GGGCTACGCC GCAGCTCATG CCTTCCGCCT AGAGCCGCTC
    ACGCCCCGCG GCGTGCTCAC GGTGGACGGG GAGCAGGTGG AGTACGGGCC ATTGCAGGCG
    CAGGTGCATC CTGGCCTCGG TACATTGCTC ACGGGTCCTC CAGGCTGCCC TGGGCGGGAA
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834995.1
    CDS245..2230
    Translation

    Target ORF information:

    RefSeq Version XM_024979227.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTGGCTCT CCCTCTCCAA CCCCTTAGTT CCCCCATGTC ACACCCTTTC TCAGTCTTTC 
    CAGAGGCCAG AGCAGGAGCT GACCTGGAGC TGGGGCTACC GGCCTAGAAG CGCCCTGGAC
    AGGGTCAAGG CCATGGCCCC CCCACCGCCA CTGGCCCCCA GCACCCCACT CCTGCATGGC
    GAGTTTGGCT CCTACCCAGA CCGCGGCCCA CGCTTCGCCC TCACTCTCAC ACCACAAGCC
    CTGCACATAC AGCGGTTGCG CCCAAAGCCC GAGGCCCGGC CCCGGGGTGG CCTGGTCCTG
    CTGACCGAGG TCTCAGGCTG CTGCACCCTG CGGAGCCGAA GCCCCCTGGA CTCAGCAGCC
    TACTTCTGCG TCTACACCTA CCCCAGGGGC CGGCGCGGGG CCCGGCGCAG AGCTGCACGC
    ACCTTCCGGG CCGACGGGGC AGCCACCTAC GAGGAGAACC GCGCTGAGGC CCAGCGCTGG
    GCCACTGCCC TCACGTGTCT GCTCCGTGGA CTGCCACTGC CTGGGGACGG GGAAATCACC
    CCGGACCTCC TGCCTAGGCC GCCCCGATTG CTCCTATTGG TCAATCCCTT TGGGGGGCGG
    GGCCTGGCCT GGCAGTGGTG TAAGAACCAT GTGCTGCCCA TGATCTCCGA AGCGGGGCTG
    TCCTTCAACC TGATCCAGAC AGAACGACAG AACCATGCCC GTGAGCTGGT CCAGGGGCTG
    AACCTGAGCG AATGGGACGG CATCGTCACA GTCTCGGGAG ATGGGCTGCT GTATGAGGTG
    CTGAATGGAC TCCTACAACG CCCCGACTGG GAAGAGGCTG TGAAGACCCC CGTGGGCATT
    CTCCCCTGTG GCTCGGGCAA TGCGTTAGCA GGAGCCGTGA ACCGGCATGG GGGATTTGAG
    CCTGCCCTGG GCATTGACCT GCTACTCAAC TGCTCCTTAC TGCTCTGCCG GGGTGGCAGC
    CACCCGCTGG ACCTGCTCTC CGTGACTCTG GCCTCCGGCT CCCGCTGTTT CTCTTTCCTG
    TCTGTGGCCT GGGGCTTCGT GTCAGATGTG GACATCCAGA GCGAGCGCTT CAGGGCCCTG
    GGCAGTGCTC GCTTCACGCT GGGCACAGTC CTGGGCCTTG CCACGCTGCA CACCTACCGC
    GGCCGCTTCT CCTACCTCCC TGCCACTGTG GAACCCGCCT CGCCTGCCCC TGCCCATGGC
    CTGCCAAGGG CCAAGTCAGA GTTGACCCTG GCCCCGGCCC CAGCCCCACC CGTGGCCCAC
    TCACCCCTGC ATCGCTCAGT GTCAGACCTG CCCCTGCCCC TGCCTCAGCC TGCCCTGACC
    TCCCCTGGCT CACCTGAACC CCTGCCCATC CTGTCTCTCA ACGGTGGGGG CCCAGAGCTG
    GCTGGGGACT GGGGTGGGGC TGGGGATGCC CCACTCTCCC CGGACCCACT GCTGCCCTCG
    CCCCCGGGGA CCCCCAAGGC AGCTCAGCTC TCACCCATCA GCGAGGGGGC CTCAGAAATG
    GCAGCATCCT CTGGGCTCCC ACCTCCCACC CCTGGTGCCC CAGTATCCAT CTCTGCTGGG
    GGACCACCTG ATCACCTGCT CCCTCCTCTA GGCACTCCGA TACCCCCAGG CTGGGTGACG
    ATGGAGGGAG ACTTTGTGCT TATTTTGGCC ATCTTACCCA GCCACCTGGG GGCTGACCTC
    GTGGCGGCCC CCCACGCGCG CTTTGACGAC GGCCTGGTGC ACCTGTGCTG GGTTCGCAGC
    GGCATCTCGC GGGCGGCGCT ACTGCGCATG TTTCTGGCCA TGGAGCGTGG TACCCACTTC
    AGCCTGGGCT GTCCGTACCT GGGCTACGCC GCAGCTCATG CCTTCCGCCT AGAGCCGCTC
    ACGCCCCGCG GCGTGCTCAC GGTGGACGGG GAGCAGGTGG AGTACGGGCC ATTGCAGGCG
    CAGGTGCATC CTGGCCTCGG TACATTGCTC ACGGGTCCTC CAGGCTGCCC TGGGCGGGAA
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225682
    Clone ID Related Accession (Same CDS sequence) XM_024979227.1 , XM_024979228.1
    Accession Version XM_024979228.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTGGCTCT CCCTCTCCAA CCCCTTAGTT CCCCCATGTC ACACCCTTTC TCAGTCTTTC 
    CAGAGGCCAG AGCAGGAGCT GACCTGGAGC TGGGGCTACC GGCCTAGAAG CGCCCTGGAC
    AGGGTCAAGG CCATGGCCCC CCCACCGCCA CTGGCCCCCA GCACCCCACT CCTGCATGGC
    GAGTTTGGCT CCTACCCAGA CCGCGGCCCA CGCTTCGCCC TCACTCTCAC ACCACAAGCC
    CTGCACATAC AGCGGTTGCG CCCAAAGCCC GAGGCCCGGC CCCGGGGTGG CCTGGTCCTG
    CTGACCGAGG TCTCAGGCTG CTGCACCCTG CGGAGCCGAA GCCCCCTGGA CTCAGCAGCC
    TACTTCTGCG TCTACACCTA CCCCAGGGGC CGGCGCGGGG CCCGGCGCAG AGCTGCACGC
    ACCTTCCGGG CCGACGGGGC AGCCACCTAC GAGGAGAACC GCGCTGAGGC CCAGCGCTGG
    GCCACTGCCC TCACGTGTCT GCTCCGTGGA CTGCCACTGC CTGGGGACGG GGAAATCACC
    CCGGACCTCC TGCCTAGGCC GCCCCGATTG CTCCTATTGG TCAATCCCTT TGGGGGGCGG
    GGCCTGGCCT GGCAGTGGTG TAAGAACCAT GTGCTGCCCA TGATCTCCGA AGCGGGGCTG
    TCCTTCAACC TGATCCAGAC AGAACGACAG AACCATGCCC GTGAGCTGGT CCAGGGGCTG
    AACCTGAGCG AATGGGACGG CATCGTCACA GTCTCGGGAG ATGGGCTGCT GTATGAGGTG
    CTGAATGGAC TCCTACAACG CCCCGACTGG GAAGAGGCTG TGAAGACCCC CGTGGGCATT
    CTCCCCTGTG GCTCGGGCAA TGCGTTAGCA GGAGCCGTGA ACCGGCATGG GGGATTTGAG
    CCTGCCCTGG GCATTGACCT GCTACTCAAC TGCTCCTTAC TGCTCTGCCG GGGTGGCAGC
    CACCCGCTGG ACCTGCTCTC CGTGACTCTG GCCTCCGGCT CCCGCTGTTT CTCTTTCCTG
    TCTGTGGCCT GGGGCTTCGT GTCAGATGTG GACATCCAGA GCGAGCGCTT CAGGGCCCTG
    GGCAGTGCTC GCTTCACGCT GGGCACAGTC CTGGGCCTTG CCACGCTGCA CACCTACCGC
    GGCCGCTTCT CCTACCTCCC TGCCACTGTG GAACCCGCCT CGCCTGCCCC TGCCCATGGC
    CTGCCAAGGG CCAAGTCAGA GTTGACCCTG GCCCCGGCCC CAGCCCCACC CGTGGCCCAC
    TCACCCCTGC ATCGCTCAGT GTCAGACCTG CCCCTGCCCC TGCCTCAGCC TGCCCTGACC
    TCCCCTGGCT CACCTGAACC CCTGCCCATC CTGTCTCTCA ACGGTGGGGG CCCAGAGCTG
    GCTGGGGACT GGGGTGGGGC TGGGGATGCC CCACTCTCCC CGGACCCACT GCTGCCCTCG
    CCCCCGGGGA CCCCCAAGGC AGCTCAGCTC TCACCCATCA GCGAGGGGGC CTCAGAAATG
    GCAGCATCCT CTGGGCTCCC ACCTCCCACC CCTGGTGCCC CAGTATCCAT CTCTGCTGGG
    GGACCACCTG ATCACCTGCT CCCTCCTCTA GGCACTCCGA TACCCCCAGG CTGGGTGACG
    ATGGAGGGAG ACTTTGTGCT TATTTTGGCC ATCTTACCCA GCCACCTGGG GGCTGACCTC
    GTGGCGGCCC CCCACGCGCG CTTTGACGAC GGCCTGGTGC ACCTGTGCTG GGTTCGCAGC
    GGCATCTCGC GGGCGGCGCT ACTGCGCATG TTTCTGGCCA TGGAGCGTGG TACCCACTTC
    AGCCTGGGCT GTCCGTACCT GGGCTACGCC GCAGCTCATG CCTTCCGCCT AGAGCCGCTC
    ACGCCCCGCG GCGTGCTCAC GGTGGACGGG GAGCAGGTGG AGTACGGGCC ATTGCAGGCG
    CAGGTGCATC CTGGCCTCGG TACATTGCTC ACGGGTCCTC CAGGCTGCCC TGGGCGGGAA
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834996.1
    CDS60..2045
    Translation

    Target ORF information:

    RefSeq Version XM_024979228.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979228.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTGGCTCT CCCTCTCCAA CCCCTTAGTT CCCCCATGTC ACACCCTTTC TCAGTCTTTC 
    CAGAGGCCAG AGCAGGAGCT GACCTGGAGC TGGGGCTACC GGCCTAGAAG CGCCCTGGAC
    AGGGTCAAGG CCATGGCCCC CCCACCGCCA CTGGCCCCCA GCACCCCACT CCTGCATGGC
    GAGTTTGGCT CCTACCCAGA CCGCGGCCCA CGCTTCGCCC TCACTCTCAC ACCACAAGCC
    CTGCACATAC AGCGGTTGCG CCCAAAGCCC GAGGCCCGGC CCCGGGGTGG CCTGGTCCTG
    CTGACCGAGG TCTCAGGCTG CTGCACCCTG CGGAGCCGAA GCCCCCTGGA CTCAGCAGCC
    TACTTCTGCG TCTACACCTA CCCCAGGGGC CGGCGCGGGG CCCGGCGCAG AGCTGCACGC
    ACCTTCCGGG CCGACGGGGC AGCCACCTAC GAGGAGAACC GCGCTGAGGC CCAGCGCTGG
    GCCACTGCCC TCACGTGTCT GCTCCGTGGA CTGCCACTGC CTGGGGACGG GGAAATCACC
    CCGGACCTCC TGCCTAGGCC GCCCCGATTG CTCCTATTGG TCAATCCCTT TGGGGGGCGG
    GGCCTGGCCT GGCAGTGGTG TAAGAACCAT GTGCTGCCCA TGATCTCCGA AGCGGGGCTG
    TCCTTCAACC TGATCCAGAC AGAACGACAG AACCATGCCC GTGAGCTGGT CCAGGGGCTG
    AACCTGAGCG AATGGGACGG CATCGTCACA GTCTCGGGAG ATGGGCTGCT GTATGAGGTG
    CTGAATGGAC TCCTACAACG CCCCGACTGG GAAGAGGCTG TGAAGACCCC CGTGGGCATT
    CTCCCCTGTG GCTCGGGCAA TGCGTTAGCA GGAGCCGTGA ACCGGCATGG GGGATTTGAG
    CCTGCCCTGG GCATTGACCT GCTACTCAAC TGCTCCTTAC TGCTCTGCCG GGGTGGCAGC
    CACCCGCTGG ACCTGCTCTC CGTGACTCTG GCCTCCGGCT CCCGCTGTTT CTCTTTCCTG
    TCTGTGGCCT GGGGCTTCGT GTCAGATGTG GACATCCAGA GCGAGCGCTT CAGGGCCCTG
    GGCAGTGCTC GCTTCACGCT GGGCACAGTC CTGGGCCTTG CCACGCTGCA CACCTACCGC
    GGCCGCTTCT CCTACCTCCC TGCCACTGTG GAACCCGCCT CGCCTGCCCC TGCCCATGGC
    CTGCCAAGGG CCAAGTCAGA GTTGACCCTG GCCCCGGCCC CAGCCCCACC CGTGGCCCAC
    TCACCCCTGC ATCGCTCAGT GTCAGACCTG CCCCTGCCCC TGCCTCAGCC TGCCCTGACC
    TCCCCTGGCT CACCTGAACC CCTGCCCATC CTGTCTCTCA ACGGTGGGGG CCCAGAGCTG
    GCTGGGGACT GGGGTGGGGC TGGGGATGCC CCACTCTCCC CGGACCCACT GCTGCCCTCG
    CCCCCGGGGA CCCCCAAGGC AGCTCAGCTC TCACCCATCA GCGAGGGGGC CTCAGAAATG
    GCAGCATCCT CTGGGCTCCC ACCTCCCACC CCTGGTGCCC CAGTATCCAT CTCTGCTGGG
    GGACCACCTG ATCACCTGCT CCCTCCTCTA GGCACTCCGA TACCCCCAGG CTGGGTGACG
    ATGGAGGGAG ACTTTGTGCT TATTTTGGCC ATCTTACCCA GCCACCTGGG GGCTGACCTC
    GTGGCGGCCC CCCACGCGCG CTTTGACGAC GGCCTGGTGC ACCTGTGCTG GGTTCGCAGC
    GGCATCTCGC GGGCGGCGCT ACTGCGCATG TTTCTGGCCA TGGAGCGTGG TACCCACTTC
    AGCCTGGGCT GTCCGTACCT GGGCTACGCC GCAGCTCATG CCTTCCGCCT AGAGCCGCTC
    ACGCCCCGCG GCGTGCTCAC GGTGGACGGG GAGCAGGTGG AGTACGGGCC ATTGCAGGCG
    CAGGTGCATC CTGGCCTCGG TACATTGCTC ACGGGTCCTC CAGGCTGCCC TGGGCGGGAA
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225683
    Clone ID Related Accession (Same CDS sequence) XM_024979231.1 , XM_024979232.1 , XM_015458245.2 , XM_024979230.1 , XM_024979229.1
    Accession Version XM_024979231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834999.1
    CDS250..2211
    Translation

    Target ORF information:

    RefSeq Version XM_024979231.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225683
    Clone ID Related Accession (Same CDS sequence) XM_024979231.1 , XM_024979232.1 , XM_015458245.2 , XM_024979230.1 , XM_024979229.1
    Accession Version XM_024979232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835000.1
    CDS277..2238
    Translation

    Target ORF information:

    RefSeq Version XM_024979232.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225683
    Clone ID Related Accession (Same CDS sequence) XM_024979231.1 , XM_024979232.1 , XM_015458245.2 , XM_024979230.1 , XM_024979229.1
    Accession Version XM_015458245.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015313731.2
    CDS363..2324
    Translation

    Target ORF information:

    RefSeq Version XM_015458245.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225683
    Clone ID Related Accession (Same CDS sequence) XM_024979231.1 , XM_024979232.1 , XM_015458245.2 , XM_024979230.1 , XM_024979229.1
    Accession Version XM_024979230.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834998.1
    CDS276..2237
    Translation

    Target ORF information:

    RefSeq Version XM_024979230.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979230.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225683
    Clone ID Related Accession (Same CDS sequence) XM_024979231.1 , XM_024979232.1 , XM_015458245.2 , XM_024979230.1 , XM_024979229.1
    Accession Version XM_024979229.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834997.1
    CDS451..2412
    Translation

    Target ORF information:

    RefSeq Version XM_024979229.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979229.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCC TGGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGCCATGGA GCGTGGTACC CACTTCAGCC TGGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCTT CCGCCTAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGGGGAGC AGGTGGAGTA CGGGCCATTG CAGGCGCAGG TGCATCCTGG CCTCGGTACA
    TTGCTCACGG GTCCTCCAGG CTGCCCTGGG CGGGAAGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225684
    Clone ID Related Accession (Same CDS sequence) XM_024979233.1
    Accession Version XM_024979233.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAACTC AGAGGCCAGA GCAGGAGCTG ACCTGGAGCT GGGGCTACCG GCCTAGAAGC 
    GCCCTGGACA GGGTCAAGGC CATGGCCCCC CCACCGCCAC TGGCCCCCAG CACCCCACTC
    CTGCATGGCG AGTTTGGCTC CTACCCAGAC CGCGGCCCAC GCTTCGCCCT CACTCTCACA
    CCACAAGCCC TGCACATACA GCGGTTGCGC CCAAAGCCCG AGGCCCGGCC CCGGGGTGGC
    CTGGTCCTGC TGACCGAGGT CTCAGGCTGC TGCACCCTGC GGAGCCGAAG CCCCCTGGAC
    TCAGCAGCCT ACTTCTGCGT CTACACCTAC CCCAGGGGCC GGCGCGGGGC CCGGCGCAGA
    GCTGCACGCA CCTTCCGGGC CGACGGGGCA GCCACCTACG AGGAGAACCG CGCTGAGGCC
    CAGCGCTGGG CCACTGCCCT CACGTGTCTG CTCCGTGGAC TGCCACTGCC TGGGGACGGG
    GAAATCACCC CGGACCTCCT GCCTAGGCCG CCCCGATTGC TCCTATTGGT CAATCCCTTT
    GGGGGGCGGG GCCTGGCCTG GCAGTGGTGT AAGAACCATG TGCTGCCCAT GATCTCCGAA
    GCGGGGCTGT CCTTCAACCT GATCCAGACA GAACGACAGA ACCATGCCCG TGAGCTGGTC
    CAGGGGCTGA ACCTGAGCGA ATGGGACGGC ATCGTCACAG TCTCGGGAGA TGGGCTGCTG
    TATGAGGTGC TGAATGGACT CCTACAACGC CCCGACTGGG AAGAGGCTGT GAAGACCCCC
    GTGGGCATTC TCCCCTGTGG CTCGGGCAAT GCGTTAGCAG GAGCCGTGAA CCGGCATGGG
    GGATTTGAGC CTGCCCTGGG CATTGACCTG CTACTCAACT GCTCCTTACT GCTCTGCCGG
    GGTGGCAGCC ACCCGCTGGA CCTGCTCTCC GTGACTCTGG CCTCCGGCTC CCGCTGTTTC
    TCTTTCCTGT CTGTGGCCTG GGGCTTCGTG TCAGATGTGG ACATCCAGAG CGAGCGCTTC
    AGGGCCCTGG GCAGTGCTCG CTTCACGCTG GGCACAGTCC TGGGCCTTGC CACGCTGCAC
    ACCTACCGCG GCCGCTTCTC CTACCTCCCT GCCACTGTGG AACCCGCCTC GCCTGCCCCT
    GCCCATGGCC TGCCAAGGGC CAAGTCAGAG TTGACCCTGG CCCCGGCCCC AGCCCCACCC
    GTGGCCCACT CACCCCTGCA TCGCTCAGTG TCAGACCTGC CCCTGCCCCT GCCTCAGCCT
    GCCCTGACCT CCCCTGGCTC ACCTGAACCC CTGCCCATCC TGTCTCTCAA CGGTGGGGGC
    CCAGAGCTGG CTGGGGACTG GGGTGGGGCT GGGGATGCCC CACTCTCCCC GGACCCACTG
    CTGCCCTCGC CCCCGGGGAC CCCCAAGGCA GCTCAGCTCT CACCCATCAG CGAGGGGGCC
    TCAGAAATGG CAGCATCCTC TGGGCTCCCA CCTCCCACCC CTGGTGCCCC AGTATCCATC
    TCTGCTGGGG GACCACCTGA TCACCTGCTC CCTCCTCTAG GCACTCCGAT ACCCCCAGGC
    TGGGTGACGA TGGAGGGAGA CTTTGTGCTT ATTTTGGCCA TCTTACCCAG CCACCTGGGG
    GCTGACCTCG TGGCGGCCCC CCACGCGCGC TTTGACGACG GCCTGGTGCA CCTGTGCTGG
    GTTCGCAGCG GCATCTCGCG GGCGGCGCTA CTGCGCATGT TTCTGGCCAT GGAGCGTGGT
    ACCCACTTCA GCCTGGGCTG TCCGTACCTG GGCTACGCCG CAGCTCATGC CTTCCGCCTA
    GAGCCGCTCA CGCCCCGCGG CGTGCTCACG GTGGACGGGG AGCAGGTGGA GTACGGGCCA
    TTGCAGGCGC AGGTGCATCC TGGCCTCGGT ACATTGCTCA CGGGTCCTCC AGGCTGCCCT
    GGGCGGGAAG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835001.1
    CDS225..2159
    Translation

    Target ORF information:

    RefSeq Version XM_024979233.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979233.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAACTC AGAGGCCAGA GCAGGAGCTG ACCTGGAGCT GGGGCTACCG GCCTAGAAGC 
    GCCCTGGACA GGGTCAAGGC CATGGCCCCC CCACCGCCAC TGGCCCCCAG CACCCCACTC
    CTGCATGGCG AGTTTGGCTC CTACCCAGAC CGCGGCCCAC GCTTCGCCCT CACTCTCACA
    CCACAAGCCC TGCACATACA GCGGTTGCGC CCAAAGCCCG AGGCCCGGCC CCGGGGTGGC
    CTGGTCCTGC TGACCGAGGT CTCAGGCTGC TGCACCCTGC GGAGCCGAAG CCCCCTGGAC
    TCAGCAGCCT ACTTCTGCGT CTACACCTAC CCCAGGGGCC GGCGCGGGGC CCGGCGCAGA
    GCTGCACGCA CCTTCCGGGC CGACGGGGCA GCCACCTACG AGGAGAACCG CGCTGAGGCC
    CAGCGCTGGG CCACTGCCCT CACGTGTCTG CTCCGTGGAC TGCCACTGCC TGGGGACGGG
    GAAATCACCC CGGACCTCCT GCCTAGGCCG CCCCGATTGC TCCTATTGGT CAATCCCTTT
    GGGGGGCGGG GCCTGGCCTG GCAGTGGTGT AAGAACCATG TGCTGCCCAT GATCTCCGAA
    GCGGGGCTGT CCTTCAACCT GATCCAGACA GAACGACAGA ACCATGCCCG TGAGCTGGTC
    CAGGGGCTGA ACCTGAGCGA ATGGGACGGC ATCGTCACAG TCTCGGGAGA TGGGCTGCTG
    TATGAGGTGC TGAATGGACT CCTACAACGC CCCGACTGGG AAGAGGCTGT GAAGACCCCC
    GTGGGCATTC TCCCCTGTGG CTCGGGCAAT GCGTTAGCAG GAGCCGTGAA CCGGCATGGG
    GGATTTGAGC CTGCCCTGGG CATTGACCTG CTACTCAACT GCTCCTTACT GCTCTGCCGG
    GGTGGCAGCC ACCCGCTGGA CCTGCTCTCC GTGACTCTGG CCTCCGGCTC CCGCTGTTTC
    TCTTTCCTGT CTGTGGCCTG GGGCTTCGTG TCAGATGTGG ACATCCAGAG CGAGCGCTTC
    AGGGCCCTGG GCAGTGCTCG CTTCACGCTG GGCACAGTCC TGGGCCTTGC CACGCTGCAC
    ACCTACCGCG GCCGCTTCTC CTACCTCCCT GCCACTGTGG AACCCGCCTC GCCTGCCCCT
    GCCCATGGCC TGCCAAGGGC CAAGTCAGAG TTGACCCTGG CCCCGGCCCC AGCCCCACCC
    GTGGCCCACT CACCCCTGCA TCGCTCAGTG TCAGACCTGC CCCTGCCCCT GCCTCAGCCT
    GCCCTGACCT CCCCTGGCTC ACCTGAACCC CTGCCCATCC TGTCTCTCAA CGGTGGGGGC
    CCAGAGCTGG CTGGGGACTG GGGTGGGGCT GGGGATGCCC CACTCTCCCC GGACCCACTG
    CTGCCCTCGC CCCCGGGGAC CCCCAAGGCA GCTCAGCTCT CACCCATCAG CGAGGGGGCC
    TCAGAAATGG CAGCATCCTC TGGGCTCCCA CCTCCCACCC CTGGTGCCCC AGTATCCATC
    TCTGCTGGGG GACCACCTGA TCACCTGCTC CCTCCTCTAG GCACTCCGAT ACCCCCAGGC
    TGGGTGACGA TGGAGGGAGA CTTTGTGCTT ATTTTGGCCA TCTTACCCAG CCACCTGGGG
    GCTGACCTCG TGGCGGCCCC CCACGCGCGC TTTGACGACG GCCTGGTGCA CCTGTGCTGG
    GTTCGCAGCG GCATCTCGCG GGCGGCGCTA CTGCGCATGT TTCTGGCCAT GGAGCGTGGT
    ACCCACTTCA GCCTGGGCTG TCCGTACCTG GGCTACGCCG CAGCTCATGC CTTCCGCCTA
    GAGCCGCTCA CGCCCCGCGG CGTGCTCACG GTGGACGGGG AGCAGGTGGA GTACGGGCCA
    TTGCAGGCGC AGGTGCATCC TGGCCTCGGT ACATTGCTCA CGGGTCCTCC AGGCTGCCCT
    GGGCGGGAAG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225685
    Clone ID Related Accession (Same CDS sequence) XM_024979235.1 , XM_024979236.1 , XM_024979234.1
    Accession Version XM_024979235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCCC CACCGCCACT GGCCCCCAGC ACCCCACTCC TGCATGGCGA GTTTGGCTCC 
    TACCCAGACC GCGGCCCACG CTTCGCCCTC ACTCTCACAC CACAAGCCCT GCACATACAG
    CGGTTGCGCC CAAAGCCCGA GGCCCGGCCC CGGGGTGGCC TGGTCCTGCT GACCGAGGTC
    TCAGGCTGCT GCACCCTGCG GAGCCGAAGC CCCCTGGACT CAGCAGCCTA CTTCTGCGTC
    TACACCTACC CCAGGGGCCG GCGCGGGGCC CGGCGCAGAG CTGCACGCAC CTTCCGGGCC
    GACGGGGCAG CCACCTACGA GGAGAACCGC GCTGAGGCCC AGCGCTGGGC CACTGCCCTC
    ACGTGTCTGC TCCGTGGACT GCCACTGCCT GGGGACGGGG AAATCACCCC GGACCTCCTG
    CCTAGGCCGC CCCGATTGCT CCTATTGGTC AATCCCTTTG GGGGGCGGGG CCTGGCCTGG
    CAGTGGTGTA AGAACCATGT GCTGCCCATG ATCTCCGAAG CGGGGCTGTC CTTCAACCTG
    ATCCAGACAG AACGACAGAA CCATGCCCGT GAGCTGGTCC AGGGGCTGAA CCTGAGCGAA
    TGGGACGGCA TCGTCACAGT CTCGGGAGAT GGGCTGCTGT ATGAGGTGCT GAATGGACTC
    CTACAACGCC CCGACTGGGA AGAGGCTGTG AAGACCCCCG TGGGCATTCT CCCCTGTGGC
    TCGGGCAATG CGTTAGCAGG AGCCGTGAAC CGGCATGGGG GATTTGAGCC TGCCCTGGGC
    ATTGACCTGC TACTCAACTG CTCCTTACTG CTCTGCCGGG GTGGCAGCCA CCCGCTGGAC
    CTGCTCTCCG TGACTCTGGC CTCCGGCTCC CGCTGTTTCT CTTTCCTGTC TGTGGCCTGG
    GGCTTCGTGT CAGATGTGGA CATCCAGAGC GAGCGCTTCA GGGCCCTGGG CAGTGCTCGC
    TTCACGCTGG GCACAGTCCT GGGCCTTGCC ACGCTGCACA CCTACCGCGG CCGCTTCTCC
    TACCTCCCTG CCACTGTGGA ACCCGCCTCG CCTGCCCCTG CCCATGGCCT GCCAAGGGCC
    AAGTCAGAGT TGACCCTGGC CCCGGCCCCA GCCCCACCCG TGGCCCACTC ACCCCTGCAT
    CGCTCAGTGT CAGACCTGCC CCTGCCCCTG CCTCAGCCTG CCCTGACCTC CCCTGGCTCA
    CCTGAACCCC TGCCCATCCT GTCTCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG
    GGTGGGGCTG GGGATGCCCC ACTCTCCCCG GACCCACTGC TGCCCTCGCC CCCGGGGACC
    CCCAAGGCAG CTCAGCTCTC ACCCATCAGC GAGGGGGCCT CAGAAATGGC AGCATCCTCT
    GGGCTCCCAC CTCCCACCCC TGGTGCCCCA GTATCCATCT CTGCTGGGGG ACCACCTGAT
    CACCTGCTCC CTCCTCTAGG CACTCCGATA CCCCCAGGCT GGGTGACGAT GGAGGGAGAC
    TTTGTGCTTA TTTTGGCCAT CTTACCCAGC CACCTGGGGG CTGACCTCGT GGCGGCCCCC
    CACGCGCGCT TTGACGACGG CCTGGTGCAC CTGTGCTGGG TTCGCAGCGG CATCTCGCGG
    GCGGCGCTAC TGCGCATGTT TCTGGCCATG GAGCGTGGTA CCCACTTCAG CCTGGGCTGT
    CCGTACCTGG GCTACGCCGC AGCTCATGCC TTCCGCCTAG AGCCGCTCAC GCCCCGCGGC
    GTGCTCACGG TGGACGGGGA GCAGGTGGAG TACGGGCCAT TGCAGGCGCA GGTGCATCCT
    GGCCTCGGTA CATTGCTCAC GGGTCCTCCA GGCTGCCCTG GGCGGGAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835003.1
    CDS497..2350
    Translation

    Target ORF information:

    RefSeq Version XM_024979235.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCCC CACCGCCACT GGCCCCCAGC ACCCCACTCC TGCATGGCGA GTTTGGCTCC 
    TACCCAGACC GCGGCCCACG CTTCGCCCTC ACTCTCACAC CACAAGCCCT GCACATACAG
    CGGTTGCGCC CAAAGCCCGA GGCCCGGCCC CGGGGTGGCC TGGTCCTGCT GACCGAGGTC
    TCAGGCTGCT GCACCCTGCG GAGCCGAAGC CCCCTGGACT CAGCAGCCTA CTTCTGCGTC
    TACACCTACC CCAGGGGCCG GCGCGGGGCC CGGCGCAGAG CTGCACGCAC CTTCCGGGCC
    GACGGGGCAG CCACCTACGA GGAGAACCGC GCTGAGGCCC AGCGCTGGGC CACTGCCCTC
    ACGTGTCTGC TCCGTGGACT GCCACTGCCT GGGGACGGGG AAATCACCCC GGACCTCCTG
    CCTAGGCCGC CCCGATTGCT CCTATTGGTC AATCCCTTTG GGGGGCGGGG CCTGGCCTGG
    CAGTGGTGTA AGAACCATGT GCTGCCCATG ATCTCCGAAG CGGGGCTGTC CTTCAACCTG
    ATCCAGACAG AACGACAGAA CCATGCCCGT GAGCTGGTCC AGGGGCTGAA CCTGAGCGAA
    TGGGACGGCA TCGTCACAGT CTCGGGAGAT GGGCTGCTGT ATGAGGTGCT GAATGGACTC
    CTACAACGCC CCGACTGGGA AGAGGCTGTG AAGACCCCCG TGGGCATTCT CCCCTGTGGC
    TCGGGCAATG CGTTAGCAGG AGCCGTGAAC CGGCATGGGG GATTTGAGCC TGCCCTGGGC
    ATTGACCTGC TACTCAACTG CTCCTTACTG CTCTGCCGGG GTGGCAGCCA CCCGCTGGAC
    CTGCTCTCCG TGACTCTGGC CTCCGGCTCC CGCTGTTTCT CTTTCCTGTC TGTGGCCTGG
    GGCTTCGTGT CAGATGTGGA CATCCAGAGC GAGCGCTTCA GGGCCCTGGG CAGTGCTCGC
    TTCACGCTGG GCACAGTCCT GGGCCTTGCC ACGCTGCACA CCTACCGCGG CCGCTTCTCC
    TACCTCCCTG CCACTGTGGA ACCCGCCTCG CCTGCCCCTG CCCATGGCCT GCCAAGGGCC
    AAGTCAGAGT TGACCCTGGC CCCGGCCCCA GCCCCACCCG TGGCCCACTC ACCCCTGCAT
    CGCTCAGTGT CAGACCTGCC CCTGCCCCTG CCTCAGCCTG CCCTGACCTC CCCTGGCTCA
    CCTGAACCCC TGCCCATCCT GTCTCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG
    GGTGGGGCTG GGGATGCCCC ACTCTCCCCG GACCCACTGC TGCCCTCGCC CCCGGGGACC
    CCCAAGGCAG CTCAGCTCTC ACCCATCAGC GAGGGGGCCT CAGAAATGGC AGCATCCTCT
    GGGCTCCCAC CTCCCACCCC TGGTGCCCCA GTATCCATCT CTGCTGGGGG ACCACCTGAT
    CACCTGCTCC CTCCTCTAGG CACTCCGATA CCCCCAGGCT GGGTGACGAT GGAGGGAGAC
    TTTGTGCTTA TTTTGGCCAT CTTACCCAGC CACCTGGGGG CTGACCTCGT GGCGGCCCCC
    CACGCGCGCT TTGACGACGG CCTGGTGCAC CTGTGCTGGG TTCGCAGCGG CATCTCGCGG
    GCGGCGCTAC TGCGCATGTT TCTGGCCATG GAGCGTGGTA CCCACTTCAG CCTGGGCTGT
    CCGTACCTGG GCTACGCCGC AGCTCATGCC TTCCGCCTAG AGCCGCTCAC GCCCCGCGGC
    GTGCTCACGG TGGACGGGGA GCAGGTGGAG TACGGGCCAT TGCAGGCGCA GGTGCATCCT
    GGCCTCGGTA CATTGCTCAC GGGTCCTCCA GGCTGCCCTG GGCGGGAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225685
    Clone ID Related Accession (Same CDS sequence) XM_024979235.1 , XM_024979236.1 , XM_024979234.1
    Accession Version XM_024979236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCCC CACCGCCACT GGCCCCCAGC ACCCCACTCC TGCATGGCGA GTTTGGCTCC 
    TACCCAGACC GCGGCCCACG CTTCGCCCTC ACTCTCACAC CACAAGCCCT GCACATACAG
    CGGTTGCGCC CAAAGCCCGA GGCCCGGCCC CGGGGTGGCC TGGTCCTGCT GACCGAGGTC
    TCAGGCTGCT GCACCCTGCG GAGCCGAAGC CCCCTGGACT CAGCAGCCTA CTTCTGCGTC
    TACACCTACC CCAGGGGCCG GCGCGGGGCC CGGCGCAGAG CTGCACGCAC CTTCCGGGCC
    GACGGGGCAG CCACCTACGA GGAGAACCGC GCTGAGGCCC AGCGCTGGGC CACTGCCCTC
    ACGTGTCTGC TCCGTGGACT GCCACTGCCT GGGGACGGGG AAATCACCCC GGACCTCCTG
    CCTAGGCCGC CCCGATTGCT CCTATTGGTC AATCCCTTTG GGGGGCGGGG CCTGGCCTGG
    CAGTGGTGTA AGAACCATGT GCTGCCCATG ATCTCCGAAG CGGGGCTGTC CTTCAACCTG
    ATCCAGACAG AACGACAGAA CCATGCCCGT GAGCTGGTCC AGGGGCTGAA CCTGAGCGAA
    TGGGACGGCA TCGTCACAGT CTCGGGAGAT GGGCTGCTGT ATGAGGTGCT GAATGGACTC
    CTACAACGCC CCGACTGGGA AGAGGCTGTG AAGACCCCCG TGGGCATTCT CCCCTGTGGC
    TCGGGCAATG CGTTAGCAGG AGCCGTGAAC CGGCATGGGG GATTTGAGCC TGCCCTGGGC
    ATTGACCTGC TACTCAACTG CTCCTTACTG CTCTGCCGGG GTGGCAGCCA CCCGCTGGAC
    CTGCTCTCCG TGACTCTGGC CTCCGGCTCC CGCTGTTTCT CTTTCCTGTC TGTGGCCTGG
    GGCTTCGTGT CAGATGTGGA CATCCAGAGC GAGCGCTTCA GGGCCCTGGG CAGTGCTCGC
    TTCACGCTGG GCACAGTCCT GGGCCTTGCC ACGCTGCACA CCTACCGCGG CCGCTTCTCC
    TACCTCCCTG CCACTGTGGA ACCCGCCTCG CCTGCCCCTG CCCATGGCCT GCCAAGGGCC
    AAGTCAGAGT TGACCCTGGC CCCGGCCCCA GCCCCACCCG TGGCCCACTC ACCCCTGCAT
    CGCTCAGTGT CAGACCTGCC CCTGCCCCTG CCTCAGCCTG CCCTGACCTC CCCTGGCTCA
    CCTGAACCCC TGCCCATCCT GTCTCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG
    GGTGGGGCTG GGGATGCCCC ACTCTCCCCG GACCCACTGC TGCCCTCGCC CCCGGGGACC
    CCCAAGGCAG CTCAGCTCTC ACCCATCAGC GAGGGGGCCT CAGAAATGGC AGCATCCTCT
    GGGCTCCCAC CTCCCACCCC TGGTGCCCCA GTATCCATCT CTGCTGGGGG ACCACCTGAT
    CACCTGCTCC CTCCTCTAGG CACTCCGATA CCCCCAGGCT GGGTGACGAT GGAGGGAGAC
    TTTGTGCTTA TTTTGGCCAT CTTACCCAGC CACCTGGGGG CTGACCTCGT GGCGGCCCCC
    CACGCGCGCT TTGACGACGG CCTGGTGCAC CTGTGCTGGG TTCGCAGCGG CATCTCGCGG
    GCGGCGCTAC TGCGCATGTT TCTGGCCATG GAGCGTGGTA CCCACTTCAG CCTGGGCTGT
    CCGTACCTGG GCTACGCCGC AGCTCATGCC TTCCGCCTAG AGCCGCTCAC GCCCCGCGGC
    GTGCTCACGG TGGACGGGGA GCAGGTGGAG TACGGGCCAT TGCAGGCGCA GGTGCATCCT
    GGCCTCGGTA CATTGCTCAC GGGTCCTCCA GGCTGCCCTG GGCGGGAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835004.1
    CDS159..2012
    Translation

    Target ORF information:

    RefSeq Version XM_024979236.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCCC CACCGCCACT GGCCCCCAGC ACCCCACTCC TGCATGGCGA GTTTGGCTCC 
    TACCCAGACC GCGGCCCACG CTTCGCCCTC ACTCTCACAC CACAAGCCCT GCACATACAG
    CGGTTGCGCC CAAAGCCCGA GGCCCGGCCC CGGGGTGGCC TGGTCCTGCT GACCGAGGTC
    TCAGGCTGCT GCACCCTGCG GAGCCGAAGC CCCCTGGACT CAGCAGCCTA CTTCTGCGTC
    TACACCTACC CCAGGGGCCG GCGCGGGGCC CGGCGCAGAG CTGCACGCAC CTTCCGGGCC
    GACGGGGCAG CCACCTACGA GGAGAACCGC GCTGAGGCCC AGCGCTGGGC CACTGCCCTC
    ACGTGTCTGC TCCGTGGACT GCCACTGCCT GGGGACGGGG AAATCACCCC GGACCTCCTG
    CCTAGGCCGC CCCGATTGCT CCTATTGGTC AATCCCTTTG GGGGGCGGGG CCTGGCCTGG
    CAGTGGTGTA AGAACCATGT GCTGCCCATG ATCTCCGAAG CGGGGCTGTC CTTCAACCTG
    ATCCAGACAG AACGACAGAA CCATGCCCGT GAGCTGGTCC AGGGGCTGAA CCTGAGCGAA
    TGGGACGGCA TCGTCACAGT CTCGGGAGAT GGGCTGCTGT ATGAGGTGCT GAATGGACTC
    CTACAACGCC CCGACTGGGA AGAGGCTGTG AAGACCCCCG TGGGCATTCT CCCCTGTGGC
    TCGGGCAATG CGTTAGCAGG AGCCGTGAAC CGGCATGGGG GATTTGAGCC TGCCCTGGGC
    ATTGACCTGC TACTCAACTG CTCCTTACTG CTCTGCCGGG GTGGCAGCCA CCCGCTGGAC
    CTGCTCTCCG TGACTCTGGC CTCCGGCTCC CGCTGTTTCT CTTTCCTGTC TGTGGCCTGG
    GGCTTCGTGT CAGATGTGGA CATCCAGAGC GAGCGCTTCA GGGCCCTGGG CAGTGCTCGC
    TTCACGCTGG GCACAGTCCT GGGCCTTGCC ACGCTGCACA CCTACCGCGG CCGCTTCTCC
    TACCTCCCTG CCACTGTGGA ACCCGCCTCG CCTGCCCCTG CCCATGGCCT GCCAAGGGCC
    AAGTCAGAGT TGACCCTGGC CCCGGCCCCA GCCCCACCCG TGGCCCACTC ACCCCTGCAT
    CGCTCAGTGT CAGACCTGCC CCTGCCCCTG CCTCAGCCTG CCCTGACCTC CCCTGGCTCA
    CCTGAACCCC TGCCCATCCT GTCTCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG
    GGTGGGGCTG GGGATGCCCC ACTCTCCCCG GACCCACTGC TGCCCTCGCC CCCGGGGACC
    CCCAAGGCAG CTCAGCTCTC ACCCATCAGC GAGGGGGCCT CAGAAATGGC AGCATCCTCT
    GGGCTCCCAC CTCCCACCCC TGGTGCCCCA GTATCCATCT CTGCTGGGGG ACCACCTGAT
    CACCTGCTCC CTCCTCTAGG CACTCCGATA CCCCCAGGCT GGGTGACGAT GGAGGGAGAC
    TTTGTGCTTA TTTTGGCCAT CTTACCCAGC CACCTGGGGG CTGACCTCGT GGCGGCCCCC
    CACGCGCGCT TTGACGACGG CCTGGTGCAC CTGTGCTGGG TTCGCAGCGG CATCTCGCGG
    GCGGCGCTAC TGCGCATGTT TCTGGCCATG GAGCGTGGTA CCCACTTCAG CCTGGGCTGT
    CCGTACCTGG GCTACGCCGC AGCTCATGCC TTCCGCCTAG AGCCGCTCAC GCCCCGCGGC
    GTGCTCACGG TGGACGGGGA GCAGGTGGAG TACGGGCCAT TGCAGGCGCA GGTGCATCCT
    GGCCTCGGTA CATTGCTCAC GGGTCCTCCA GGCTGCCCTG GGCGGGAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225685
    Clone ID Related Accession (Same CDS sequence) XM_024979235.1 , XM_024979236.1 , XM_024979234.1
    Accession Version XM_024979234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCCC CACCGCCACT GGCCCCCAGC ACCCCACTCC TGCATGGCGA GTTTGGCTCC 
    TACCCAGACC GCGGCCCACG CTTCGCCCTC ACTCTCACAC CACAAGCCCT GCACATACAG
    CGGTTGCGCC CAAAGCCCGA GGCCCGGCCC CGGGGTGGCC TGGTCCTGCT GACCGAGGTC
    TCAGGCTGCT GCACCCTGCG GAGCCGAAGC CCCCTGGACT CAGCAGCCTA CTTCTGCGTC
    TACACCTACC CCAGGGGCCG GCGCGGGGCC CGGCGCAGAG CTGCACGCAC CTTCCGGGCC
    GACGGGGCAG CCACCTACGA GGAGAACCGC GCTGAGGCCC AGCGCTGGGC CACTGCCCTC
    ACGTGTCTGC TCCGTGGACT GCCACTGCCT GGGGACGGGG AAATCACCCC GGACCTCCTG
    CCTAGGCCGC CCCGATTGCT CCTATTGGTC AATCCCTTTG GGGGGCGGGG CCTGGCCTGG
    CAGTGGTGTA AGAACCATGT GCTGCCCATG ATCTCCGAAG CGGGGCTGTC CTTCAACCTG
    ATCCAGACAG AACGACAGAA CCATGCCCGT GAGCTGGTCC AGGGGCTGAA CCTGAGCGAA
    TGGGACGGCA TCGTCACAGT CTCGGGAGAT GGGCTGCTGT ATGAGGTGCT GAATGGACTC
    CTACAACGCC CCGACTGGGA AGAGGCTGTG AAGACCCCCG TGGGCATTCT CCCCTGTGGC
    TCGGGCAATG CGTTAGCAGG AGCCGTGAAC CGGCATGGGG GATTTGAGCC TGCCCTGGGC
    ATTGACCTGC TACTCAACTG CTCCTTACTG CTCTGCCGGG GTGGCAGCCA CCCGCTGGAC
    CTGCTCTCCG TGACTCTGGC CTCCGGCTCC CGCTGTTTCT CTTTCCTGTC TGTGGCCTGG
    GGCTTCGTGT CAGATGTGGA CATCCAGAGC GAGCGCTTCA GGGCCCTGGG CAGTGCTCGC
    TTCACGCTGG GCACAGTCCT GGGCCTTGCC ACGCTGCACA CCTACCGCGG CCGCTTCTCC
    TACCTCCCTG CCACTGTGGA ACCCGCCTCG CCTGCCCCTG CCCATGGCCT GCCAAGGGCC
    AAGTCAGAGT TGACCCTGGC CCCGGCCCCA GCCCCACCCG TGGCCCACTC ACCCCTGCAT
    CGCTCAGTGT CAGACCTGCC CCTGCCCCTG CCTCAGCCTG CCCTGACCTC CCCTGGCTCA
    CCTGAACCCC TGCCCATCCT GTCTCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG
    GGTGGGGCTG GGGATGCCCC ACTCTCCCCG GACCCACTGC TGCCCTCGCC CCCGGGGACC
    CCCAAGGCAG CTCAGCTCTC ACCCATCAGC GAGGGGGCCT CAGAAATGGC AGCATCCTCT
    GGGCTCCCAC CTCCCACCCC TGGTGCCCCA GTATCCATCT CTGCTGGGGG ACCACCTGAT
    CACCTGCTCC CTCCTCTAGG CACTCCGATA CCCCCAGGCT GGGTGACGAT GGAGGGAGAC
    TTTGTGCTTA TTTTGGCCAT CTTACCCAGC CACCTGGGGG CTGACCTCGT GGCGGCCCCC
    CACGCGCGCT TTGACGACGG CCTGGTGCAC CTGTGCTGGG TTCGCAGCGG CATCTCGCGG
    GCGGCGCTAC TGCGCATGTT TCTGGCCATG GAGCGTGGTA CCCACTTCAG CCTGGGCTGT
    CCGTACCTGG GCTACGCCGC AGCTCATGCC TTCCGCCTAG AGCCGCTCAC GCCCCGCGGC
    GTGCTCACGG TGGACGGGGA GCAGGTGGAG TACGGGCCAT TGCAGGCGCA GGTGCATCCT
    GGCCTCGGTA CATTGCTCAC GGGTCCTCCA GGCTGCCCTG GGCGGGAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835002.1
    CDS788..2641
    Translation

    Target ORF information:

    RefSeq Version XM_024979234.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCCC CACCGCCACT GGCCCCCAGC ACCCCACTCC TGCATGGCGA GTTTGGCTCC 
    TACCCAGACC GCGGCCCACG CTTCGCCCTC ACTCTCACAC CACAAGCCCT GCACATACAG
    CGGTTGCGCC CAAAGCCCGA GGCCCGGCCC CGGGGTGGCC TGGTCCTGCT GACCGAGGTC
    TCAGGCTGCT GCACCCTGCG GAGCCGAAGC CCCCTGGACT CAGCAGCCTA CTTCTGCGTC
    TACACCTACC CCAGGGGCCG GCGCGGGGCC CGGCGCAGAG CTGCACGCAC CTTCCGGGCC
    GACGGGGCAG CCACCTACGA GGAGAACCGC GCTGAGGCCC AGCGCTGGGC CACTGCCCTC
    ACGTGTCTGC TCCGTGGACT GCCACTGCCT GGGGACGGGG AAATCACCCC GGACCTCCTG
    CCTAGGCCGC CCCGATTGCT CCTATTGGTC AATCCCTTTG GGGGGCGGGG CCTGGCCTGG
    CAGTGGTGTA AGAACCATGT GCTGCCCATG ATCTCCGAAG CGGGGCTGTC CTTCAACCTG
    ATCCAGACAG AACGACAGAA CCATGCCCGT GAGCTGGTCC AGGGGCTGAA CCTGAGCGAA
    TGGGACGGCA TCGTCACAGT CTCGGGAGAT GGGCTGCTGT ATGAGGTGCT GAATGGACTC
    CTACAACGCC CCGACTGGGA AGAGGCTGTG AAGACCCCCG TGGGCATTCT CCCCTGTGGC
    TCGGGCAATG CGTTAGCAGG AGCCGTGAAC CGGCATGGGG GATTTGAGCC TGCCCTGGGC
    ATTGACCTGC TACTCAACTG CTCCTTACTG CTCTGCCGGG GTGGCAGCCA CCCGCTGGAC
    CTGCTCTCCG TGACTCTGGC CTCCGGCTCC CGCTGTTTCT CTTTCCTGTC TGTGGCCTGG
    GGCTTCGTGT CAGATGTGGA CATCCAGAGC GAGCGCTTCA GGGCCCTGGG CAGTGCTCGC
    TTCACGCTGG GCACAGTCCT GGGCCTTGCC ACGCTGCACA CCTACCGCGG CCGCTTCTCC
    TACCTCCCTG CCACTGTGGA ACCCGCCTCG CCTGCCCCTG CCCATGGCCT GCCAAGGGCC
    AAGTCAGAGT TGACCCTGGC CCCGGCCCCA GCCCCACCCG TGGCCCACTC ACCCCTGCAT
    CGCTCAGTGT CAGACCTGCC CCTGCCCCTG CCTCAGCCTG CCCTGACCTC CCCTGGCTCA
    CCTGAACCCC TGCCCATCCT GTCTCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG
    GGTGGGGCTG GGGATGCCCC ACTCTCCCCG GACCCACTGC TGCCCTCGCC CCCGGGGACC
    CCCAAGGCAG CTCAGCTCTC ACCCATCAGC GAGGGGGCCT CAGAAATGGC AGCATCCTCT
    GGGCTCCCAC CTCCCACCCC TGGTGCCCCA GTATCCATCT CTGCTGGGGG ACCACCTGAT
    CACCTGCTCC CTCCTCTAGG CACTCCGATA CCCCCAGGCT GGGTGACGAT GGAGGGAGAC
    TTTGTGCTTA TTTTGGCCAT CTTACCCAGC CACCTGGGGG CTGACCTCGT GGCGGCCCCC
    CACGCGCGCT TTGACGACGG CCTGGTGCAC CTGTGCTGGG TTCGCAGCGG CATCTCGCGG
    GCGGCGCTAC TGCGCATGTT TCTGGCCATG GAGCGTGGTA CCCACTTCAG CCTGGGCTGT
    CCGTACCTGG GCTACGCCGC AGCTCATGCC TTCCGCCTAG AGCCGCTCAC GCCCCGCGGC
    GTGCTCACGG TGGACGGGGA GCAGGTGGAG TACGGGCCAT TGCAGGCGCA GGTGCATCCT
    GGCCTCGGTA CATTGCTCAC GGGTCCTCCA GGCTGCCCTG GGCGGGAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94996
    Clone ID Related Accession (Same CDS sequence) XM_005195452.2
    Accession Version XM_005195452.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 3 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCN CTGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGGCCATGG AGCGTGGTAC CCACTTCAGC CTGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCGT CCCGCCAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGCGGGAG CAGGTGAGAA ATACGGGCCA TTGCAGGCGC AGCGTGCATC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195509.2
    CDS335..2248
    Translation

    Target ORF information:

    RefSeq Version XM_005195452.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAATGGAA ACCTTGAAGC AGAGGAGCGG CAGGACCAGA GGCCAGAGCA GGAGCTGACC 
    TGGAGCTGGG GCTACCGGCC TAGAAGCGCC CTGGACAGGG TCAAGGCCAT GGCCCCCCCA
    CCGCCACTGG CCCCCAGCAC CCCACTCCTG CATGGCGAGT TTGGCTCCTA CCCAGACCGC
    GGCCCACGCT TCGCCCTCAC TCTCACACCA CAAGCCCTGC ACATACAGCG GTTGCGCCCA
    AAGCCCGAGG CCCGGCCCCG GGGTGGCCTG GTCCTGCTGA CCGAGGTCTC AGGCTGCTGC
    ACCCTGCGGA GCCGAAGCCC CCTGGACTCA GCAGCCTACT TCTGCGTCTA CACCTACCCC
    AGGGGCCGGC GCGGGGCCCG GCGCAGAGCT GCACGCACCT TCCGGGCCGA CGGGGCAGCC
    ACCTACGAGG AGAACCGCGC TGAGGCCCAG CGCTGGGCCA CTGCCCTCAC GTGTCTGCTC
    CGTGGACTGC CACTGCCTGG GGACGGGGAA ATCACCCCGG ACCTCCTGCC TAGGCCGCCC
    CGATTGCTCC TATTGGTCAA TCCCTTTGGG GGGCGGGGCC TGGCCTGGCA GTGGTGTAAG
    AACCATGTGC TGCCCATGAT CTCCGAAGCG GGGCTGTCCT TCAACCTGAT CCAGACAGAA
    CGACAGAACC ATGCCCGTGA GCTGGTCCAG GGGCTGAACC TGAGCGAATG GGACGGCATC
    GTCACAGTCT CGGGAGATGG GCTGCTGTAT GAGGTGCTGA ATGGACTCCT ACAACGCCCC
    GACTGGGAAG AGGCTGTGAA GACCCCCGTG GGCATTCTCC CCTGTGGCTC GGGCAATGCG
    TTAGCAGGAG CCGTGAACCG GCATGGGGGA TTTGAGCCTG CCCTGGGCAT TGACCTGCTA
    CTCAACTGCT CCTTACTGCT CTGCCGGGGT GGCAGCCACC CGCTGGACCT GCTCTCCGTG
    ACTCTGGCCT CCGGCTCCCG CTGTTTCTCT TTCCTGTCTG TGGCCTGGGG CTTCGTGTCA
    GATGTGGACA TCCAGAGCGA GCGCTTCAGG GCCCTGGGCA GTGCTCGCTT CACGCTGGGC
    ACAGTCCTGG GCCTTGCCAC GCTGCACACC TACCGCGGCC GCTTCTCCTA CCTCCCTGCC
    ACTGTGGAAC CCGCCTCGCC TGCCCCTGCC CATGGCCTGC CAAGGGCCAA GTCAGAGTTG
    ACCCTGGCCC CGGCCCCAGC CCCACCCGTG GCCCACTCAC CCCTGCATCG CTCAGTGTCA
    GACCTGCCCC TGCCCCTGCC TCAGCCTGCC CTGACCTCCC CTGGCTCACC TGAACCCCTG
    CCCATCCTGT CTCTCAACGG TGGGGGCCCA GAGCTGGCTG GGGACTGGGG TGGGGCTGGG
    GATGCCCCAC TCTCCCCGGA CCCACTGCTG CCCTCGCCCC CGGGGACCCC CAAGGCAGCT
    CAGCTCTCAC CCATCAGCGA GGGGGCCTCA GAAATGGCAG CATCCTCTGG GCTCCCACCT
    CCCACCCCTG GTGCCCCAGT ATCCATCTCT GCTGGGGGAC CACCTGATCA CCTGCTCCCT
    CCTCTAGGCA CTCCGATACC CCCAGGCTGG GTGACGATGG AGGGAGACTT TGTGCTTATT
    TTGGCCATCT TACCCAGCCA CCTGGGGGCT GACCTCGTGG CGGCCCCCCA CGCGCGCTTT
    GACGACGGCN CTGTGCACCT GTGCTGGGTT CGCAGCGGCA TCTCGCGGGC GGCGCTACTG
    CGCATGTTTC TGGGCCATGG AGCGTGGTAC CCACTTCAGC CTGGCTGTCC GTACCTGGGC
    TACGCCGCAG CTCATGCCGT CCCGCCAGAG CCGCTCACGC CCCGCGGCGT GCTCACGGTG
    GACGCGGGAG CAGGTGAGAA ATACGGGCCA TTGCAGGCGC AGCGTGCATC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.