gRNAs
The following CENPF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa36388 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001256586.1 | |
| Accession Version | NM_001256586.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 9240bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-01-03 | |
| Organism | Bos taurus(cattle) | |
| Product | centromere protein F | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03017426.1, AAFC03031658.1 and AAFC03031660.1. On or before Feb 12, 2012 this sequence version replaced XM_612376.5, XM_002694283.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403656 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001243515.1 |
| CDS | 148..9387 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001256586.1 |
1 | ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa223624 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024976312.1 | |
| Accession Version | XM_024976312.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 9564bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | centromere protein F isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAATAT GTGAAAATCT GGAGAAGACT AAGCAGAAGA TTTCTCATGA ACTTCAAGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024832080.1 |
| CDS | 198..9761 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024976312.1 |
1 | ATGGAAATAT GTGAAAATCT GGAGAAGACT AAGCAGAAGA TTTCTCATGA ACTTCAAGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa27521 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005217369.4 , XM_005217369.3 | |
| Accession Version | XM_005217369.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 9780bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | centromere protein F isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217369.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005217426.1 |
| CDS | 199..9978 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005217369.4 |
1 | ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa27521 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005217369.4 , XM_005217369.3 | |
| Accession Version | XM_005217369.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 9780bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | centromere protein F isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005217426.1 |
| CDS | 241..10020 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005217369.3 |
1 | ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage. |
A whole-genome assembly of the domestic cow, Bos taurus. |
Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F. |
Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death. |
Mitosin/CENP-F as a negative regulator of activating transcription factor-4. |
