IARS2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS2
Entrez Gene ID	533057
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
General protein information	Preferred Names isoleucyl-tRNA synthetase 2, mitochondrial Names isoleucine--tRNA ligase, mitochondrial isoleucine-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206795.1	NP_001193724.1	isoleucine--tRNA ligase, mitochondrial

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5414	NP_648837.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003156	XP_312846.5
Eremothecium gossypii	AGOS_AGL247C	NP_986420.1
Neurospora crassa	NCU00920	XP_964746.2
Arabidopsis thaliana (thale cress)	OVA2	NP_199714.2
Canis lupus familiaris (dog)	IARS2	XP_536121.3
Gallus gallus (chicken)	IARS2	NP_001006397.1
Saccharomyces cerevisiae (baker's yeast)	ISM1	NP_015285.1
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.08c	NP_587938.1
Homo sapiens (human)	IARS2	NP_060530.3
Pan troglodytes (chimpanzee)	IARS2	XP_514209.2
	IARS2	XP_001099877.1
Danio rerio (zebrafish)	iars2	NP_001157270.1
Caenorhabditis elegans (roundworm)	iars-2	NP_001251317.1
Kluyveromyces lactis	KLLA0D11858g	XP_453590.1
Xenopus tropicalis (tropical clawed frog)	iars2	NP_001120515.1
Bos taurus (cattle)	IARS2	NP_001193724.1
Mus musculus (house mouse)	Iars2	NP_941055.1
Rattus norvegicus (Norway rat)	Iars2	XP_006250494.1
Magnaporthe oryzae (rice blast fungus)	MGG_01165	XP_003714047.1
Oryza sativa (rice)	Os02g0778200	NP_001048293.1

Gene Ontology
Bibliography

Related articles in PubMed

IARS mutation causes prenatal death in Japanese Black cattle.
Hirano T, Matsuhashi T, Takeda K, Hara H, Kobayashi N, Kita K, Sugimoto Y, Hanzawa K
Animal science journal = Nihon chikusan Gakkaiho87(9)1178-81(2016 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Its mutation causes prenatal death in Japanese Black cattle
Title: IARS mutation causes prenatal death in Japanese Black cattle.

The following IARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa39650	NM_001206795.1 Latest version!	Bos taurus isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS2 ( NM_001206795.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193724.1 Bos taurus isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

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CloneID	OBa39650
Clone ID Related Accession (Same CDS sequence)	NM_001206795.1
Accession Version	NM_001206795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC102830.1, DV887098.1, DV894430.1 and DT812568.1. On or before May 6, 2011 this sequence version replaced XM_612328.4, XM_002693960.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGTTGGG GGCTGCGCCC TCGCGGTCCG GGGGAAGCGG CCCTGGCCGC GGCCTGGAAT 
TTGTGGGTGC CACCCCGCCT TCCTTGTCGA CCTCGCTGGC GAGGGACAAC TGGGAGCCTT 
TTGGTGCGGT CAGTCACCGG GGCCAGTAAC CAGCCGCAGA ACTCGAGTAA TGGCAGCTAT 
CGGGACACGG TGTTGCTGCC GCAGACCAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCCGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCTTGGCAA 
AGAGAAAGAA AAGTGAAGGC TGAATTTTGT CTTCATGATG GACCTCCTTA TGCAAATGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCGAA TCGATACCAT 
ATGATGAGGG GTTCCAAAAT ACATTTTGTT CCAGGCTGGG ATTGTCATGG GTTACCCATT 
GAAATCAAAG TATTATCAGA ACTTGGTGGA AAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAGAGAAAG CTAGATCATT CGCCAAAGCG GCAATTGAGA AACAGAAATT GTCATTTATT 
CGCTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CTTTTGATGG GAGATACGAG 
GCCAAGCAGT TGAGAATTTT CTACCAAATG TATGATAAGG GCTTGATTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC TTCATCAAGG ACTGCATTGG CTGAAGCAGA ACTTGAATAT 
AATCCGGAGC ACATCAGTCG CTCAGTGTAT GTAAAATTTC CTCTCCTGAA ACCTTCTCCT 
AAGTTGGCAT CTCTTCTAGA TGGTTCATCT CCTGTTAGTT TTTTAGTCTG GACCACACAG 
CCTTGGACAA TTCCAGCCAA TCAAGCTGTT TGTTACATGC CAGAATCAAA GTATGCTGTT 
GTGAAGTGTT CCTCATCTGG AGACTTGTAC ATTCTAGGTG CAGATAAAGT AACATCTGTT 
GCTTCTACTT TGGGAACAAC ATTTGAAGTT ATTTCAACAT TTTCAGGTGT GGACTTGGAA 
AATGGTTCTT GTGCTCATCC TTTAATTTCC GAGAGGGAGT CTCCTCTTTT ACCTGCCAAT 
CATGTGACCA TGACAAAAGG CACCGGGCTG GTTCACACAG CCCCGGCTCA TGGTATGGAA 
GATTACAGTG TAGCTGCTCA ACACAACTTG TCCACGGATT GTTTAGTGAA TGAAGATGGA 
GTTTTCACAG ATGCTGCAGG TCCTGAACTT CAAAACAAAG CTGTTCTTAA AGAGGGAACA 
GATGCAGTTA TAATGATGCT TCAGGCTGCA AAGAATTTGT TGAAAGAAGA AAAATTGGTA 
CACAGCTATC CCTATGACTG GAGAACTAAG AAACCAGTAG TTATTCGTGC CAGCAAGCAG 
TGGTTTGTAA ATATCACGGA TATTAAGACT ACAGCCAAGG AATTGTTGAA AAAAGTGAAA 
TTTATTCCTG GATCAGCATT GAATGCCATG GTTGAAATGA TGGACAGGCG GCCATACTGG 
TGCATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACAAAA 
GATGACGTCT TGATCAACAG CCAAACCATC GAGCACATTA TTCAACTAGT GGAAGAGCAT 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCG GAGCAACTTC TTCCGAAAGA AGTCTTATCT 
CAGGTCGGTG GTCCCGATGC GTTGGAGTAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGTG GAACTTCATG GTCTAATGTT CTCCCAGGTA CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTTGGGGGG TGGTTTCAGT CTTCCTTATT AACAAGTGTG 
GCAGCAAGGA ATAAGTCGCC TTTTAAGACA GTGGTTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATATCATTG ATCCTGATGT TGTCATCAAT 
GGAGGACAAG ATCACAGCAA AGAGCCCCCC TATGGTGCTG ACGTGCTTCG CTGGTGGGTT 
GCCGAGTCCA ACGTCTTCAC TGAAGTCACA ATTAGTCCAT CTCTACTGAA CGCTGCCAGA 
GATGACATTA GCAAGCTTAG GAATACACTC CGCTTTCTTT TGGGAAATGT GGCTGGTTTC 
AACCCAGAAA CAGATTCCAT CCCTGTCAAC AGCATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT AATGATTCAT ATAAGCAGTA TGACTTTGGA 
AAGGTTGTTC GGCTTTTACA AGCATTTTAT ACCAGGGAAC TCTCCAATTT TTATTTCAGC 
ATAATCAAAG ACAGGCTTTA TTGTGAAAAT GCAGATGATC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG CTGAAATTTT GGATGTAATG GTTCGCTCTT TTGCTCCCAT TCTTCCTCAC 
TTGGCTGAAG AGGTATTCCA GCACATACCC TATATTAAAG AACCCAAGAG TGTTTTTCGT 
ACTGGGTGGA TTAAAACCAG TTCTATCTGG AAGAAACCTG GACTGGAAGA AGCCATAGAA 
AGCGCGTGTG CAATGAGAGA TTCATTTCTT GGAAGCATTC CTGGCAAAAA TGCAGCTGAG 
TATGAGGTGA TCATTGTGAT TGAGCCTGGG CTGCTTTTTG AGATCATGGA GATGCTACAA 
GCTGAGGAGA CTTCCAGTAC CTCTCAGTTG AATGAATTAA TGATGGCGTC CCAGACAACT 
TTGCTTTCTC AGGAACCACG AGGAATAAGT GAGGATGTGA CTGAGCTTAA AGGGACATTT 
CTAATCAATT TAGAAGGTGG CGATATTCGT GAAGAGTCTT CATACAAAGT AATTGTCATG 
ACAACTACGA AGGAAAAATG CCCTCGCTGT TGGAAGTACA CGGCTGAGTC ATCAGACACA 
CTGTGTCCCC GATGCGCAGA AGTTGTGAAA GGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193724.1
CDS	63..3101
Translation	MRWGLRPRGPGEAALAAAWNLWVPPRLPCRPRWRGTTGSLLVRSVTGASNQPQNSSNGSYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKAEFCLHDGPPYANGDPHVGHALNKILKDIANRYHMMRGSKIHFVPGWDCHGLPIEIKVLSELGGKAQNLSAMEIREKARSFAKAAIEKQKLSFIRWGIMADWNNCYYTFDGRYEAKQLRIFYQMYDKGLIYRSYKPVFWSPSSRTALAEAELEYNPEHISRSVYVKFPLLKPSPKLASLLDGSSPVSFLVWTTQPWTIPANQAVCYMPESKYAVVKCSSSGDLYILGADKVTSVASTLGTTFEVISTFSGVDLENGSCAHPLISERESPLLPANHVTMTKGTGLVHTAPAHGMEDYSVAAQHNLSTDCLVNEDGVFTDAAGPELQNKAVLKEGTDAVIMMLQAAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFVNITDIKTTAKELLKKVKFIPGSALNAMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDDVLINSQTIEHIIQLVEEHGSDIWWTLPPEQLLPKEVLSQVGGPDALEYVPGQDILDIWFDSGTSWSNVLPGTDQRADLYLEGKDQLGGWFQSSLLTSVAARNKSPFKTVVVHGFTLGEKGEKMSKSLGNIIDPDVVINGGQDHSKEPPYGADVLRWWVAESNVFTEVTISPSLLNAARDDISKLRNTLRFLLGNVAGFNPETDSIPVNSMYVIDQYMLHLLQDLANKINDSYKQYDFGKVVRLLQAFYTRELSNFYFSIIKDRLYCENADDPKRRSCQTALAEILDVMVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWIKTSSIWKKPGLEEAIESACAMRDSFLGSIPGKNAAEYEVIIVIEPGLLFEIMEMLQAEETSSTSQLNELMMASQTTLLSQEPRGISEDVTELKGTFLINLEGGDIREESSYKVIVMTTTKEKCPRCWKYTAESSDTLCPRCAEVVKGK

Target ORF information:

RefSeq Version	NM_001206795.1
Organism	Bos taurus(cattle)
Definition	Bos taurus isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206795.1

ORF Insert Sequence:

ATGCGTTGGG GGCTGCGCCC TCGCGGTCCG GGGGAAGCGG CCCTGGCCGC GGCCTGGAAT 
TTGTGGGTGC CACCCCGCCT TCCTTGTCGA CCTCGCTGGC GAGGGACAAC TGGGAGCCTT 
TTGGTGCGGT CAGTCACCGG GGCCAGTAAC CAGCCGCAGA ACTCGAGTAA TGGCAGCTAT 
CGGGACACGG TGTTGCTGCC GCAGACCAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCCGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCTTGGCAA 
AGAGAAAGAA AAGTGAAGGC TGAATTTTGT CTTCATGATG GACCTCCTTA TGCAAATGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCGAA TCGATACCAT 
ATGATGAGGG GTTCCAAAAT ACATTTTGTT CCAGGCTGGG ATTGTCATGG GTTACCCATT 
GAAATCAAAG TATTATCAGA ACTTGGTGGA AAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAGAGAAAG CTAGATCATT CGCCAAAGCG GCAATTGAGA AACAGAAATT GTCATTTATT 
CGCTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CTTTTGATGG GAGATACGAG 
GCCAAGCAGT TGAGAATTTT CTACCAAATG TATGATAAGG GCTTGATTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC TTCATCAAGG ACTGCATTGG CTGAAGCAGA ACTTGAATAT 
AATCCGGAGC ACATCAGTCG CTCAGTGTAT GTAAAATTTC CTCTCCTGAA ACCTTCTCCT 
AAGTTGGCAT CTCTTCTAGA TGGTTCATCT CCTGTTAGTT TTTTAGTCTG GACCACACAG 
CCTTGGACAA TTCCAGCCAA TCAAGCTGTT TGTTACATGC CAGAATCAAA GTATGCTGTT 
GTGAAGTGTT CCTCATCTGG AGACTTGTAC ATTCTAGGTG CAGATAAAGT AACATCTGTT 
GCTTCTACTT TGGGAACAAC ATTTGAAGTT ATTTCAACAT TTTCAGGTGT GGACTTGGAA 
AATGGTTCTT GTGCTCATCC TTTAATTTCC GAGAGGGAGT CTCCTCTTTT ACCTGCCAAT 
CATGTGACCA TGACAAAAGG CACCGGGCTG GTTCACACAG CCCCGGCTCA TGGTATGGAA 
GATTACAGTG TAGCTGCTCA ACACAACTTG TCCACGGATT GTTTAGTGAA TGAAGATGGA 
GTTTTCACAG ATGCTGCAGG TCCTGAACTT CAAAACAAAG CTGTTCTTAA AGAGGGAACA 
GATGCAGTTA TAATGATGCT TCAGGCTGCA AAGAATTTGT TGAAAGAAGA AAAATTGGTA 
CACAGCTATC CCTATGACTG GAGAACTAAG AAACCAGTAG TTATTCGTGC CAGCAAGCAG 
TGGTTTGTAA ATATCACGGA TATTAAGACT ACAGCCAAGG AATTGTTGAA AAAAGTGAAA 
TTTATTCCTG GATCAGCATT GAATGCCATG GTTGAAATGA TGGACAGGCG GCCATACTGG 
TGCATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACAAAA 
GATGACGTCT TGATCAACAG CCAAACCATC GAGCACATTA TTCAACTAGT GGAAGAGCAT 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCG GAGCAACTTC TTCCGAAAGA AGTCTTATCT 
CAGGTCGGTG GTCCCGATGC GTTGGAGTAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGTG GAACTTCATG GTCTAATGTT CTCCCAGGTA CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTTGGGGGG TGGTTTCAGT CTTCCTTATT AACAAGTGTG 
GCAGCAAGGA ATAAGTCGCC TTTTAAGACA GTGGTTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATATCATTG ATCCTGATGT TGTCATCAAT 
GGAGGACAAG ATCACAGCAA AGAGCCCCCC TATGGTGCTG ACGTGCTTCG CTGGTGGGTT 
GCCGAGTCCA ACGTCTTCAC TGAAGTCACA ATTAGTCCAT CTCTACTGAA CGCTGCCAGA 
GATGACATTA GCAAGCTTAG GAATACACTC CGCTTTCTTT TGGGAAATGT GGCTGGTTTC 
AACCCAGAAA CAGATTCCAT CCCTGTCAAC AGCATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT AATGATTCAT ATAAGCAGTA TGACTTTGGA 
AAGGTTGTTC GGCTTTTACA AGCATTTTAT ACCAGGGAAC TCTCCAATTT TTATTTCAGC 
ATAATCAAAG ACAGGCTTTA TTGTGAAAAT GCAGATGATC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG CTGAAATTTT GGATGTAATG GTTCGCTCTT TTGCTCCCAT TCTTCCTCAC 
TTGGCTGAAG AGGTATTCCA GCACATACCC TATATTAAAG AACCCAAGAG TGTTTTTCGT 
ACTGGGTGGA TTAAAACCAG TTCTATCTGG AAGAAACCTG GACTGGAAGA AGCCATAGAA 
AGCGCGTGTG CAATGAGAGA TTCATTTCTT GGAAGCATTC CTGGCAAAAA TGCAGCTGAG 
TATGAGGTGA TCATTGTGAT TGAGCCTGGG CTGCTTTTTG AGATCATGGA GATGCTACAA 
GCTGAGGAGA CTTCCAGTAC CTCTCAGTTG AATGAATTAA TGATGGCGTC CCAGACAACT 
TTGCTTTCTC AGGAACCACG AGGAATAAGT GAGGATGTGA CTGAGCTTAA AGGGACATTT 
CTAATCAATT TAGAAGGTGG CGATATTCGT GAAGAGTCTT CATACAAAGT AATTGTCATG 
ACAACTACGA AGGAAAAATG CCCTCGCTGT TGGAAGTACA CGGCTGAGTC ATCAGACACA 
CTGTGTCCCC GATGCGCAGA AGTTGTGAAA GGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IARS mutation causes prenatal death in Japanese Black cattle.
Animal science journal = Nihon chikusan Gakkaiho87(9)1178-81(2016 Sep)
Hirano T,Matsuhashi T,Takeda K,Hara H,Kobayashi N,Kita K,Sugimoto Y,Hanzawa K

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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IARS2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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