NRAP cDNA ORF clone, Bos taurus(cattle)

The following NRAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa52266 XM_010820038.2
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $1021.30
$1459.00
OBa230890 XM_005225745.3
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230891 XM_005225746.3
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230892 XM_010820038.3
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230893 XM_005225747.3
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230894 XM_024985666.1
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa230895 XM_024985667.1
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa40533 NM_001098072.1
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa33734 XM_005225745.2
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa33735 XM_005225746.2
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa33736 XM_005225747.2
Latest version!
Bos taurus nebulin related anchoring protein (NRAP), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa52266
    Clone ID Related Accession (Same CDS sequence) XM_010820038.2
    Accession Version XM_010820038.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCATTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAGAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCATG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACGGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC GCTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GCAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC AGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ATGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCACGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCCCAT GCCCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818340.1
    CDS181..5271
    Translation

    Target ORF information:

    RefSeq Version XM_010820038.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820038.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCATTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAGAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCATG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACGGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC GCTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GCAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC AGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ATGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCACGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCCCAT GCCCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230890
    Clone ID Related Accession (Same CDS sequence) XM_005225745.3
    Accession Version XM_005225745.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCGTTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAAAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCACG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACAGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAACCAATA CAGGGCAGAT GTGAAGTGGA TGAAAGGGAC GGGCTGGGTC
    GCCACCGGGT CATTAAATGT GGAGCAGGCG AAGAAGGCAG GAGAACTCAT TAGCGAGAAG
    AAGTACCGGC AGCATCCAGA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCGGAGATG
    GTCCAGGCCA GAATTAGTTA TACCCAAGCA GTGGATAGGT TGTACCGAGA GCAAGGAGAA
    AACCTAAAGC ATCACTACAC GCAGACCACT GACCTCCCTG AAGTCCTGCT GGCCAAGCTG
    AATGCCATGA ATATCAGCGA GACACGTTAT AAGGAATCCT GGAGCAAACT TCGAGACGGT
    GGCTATAAGC TGAGACTGGA TGCCATTCCA TTCCAGGCAG CAAAGGCTTC TGGCGAAATC
    ATAAGTGATT ACAAATACAA AGAGGCATTT GAGAAAATGA AAGGGCAGAT GCTCGGCTCC
    CGGAGCTTGG AAGATGATAT CAGCCTTGCG CATTCAGTGT ACGCGAGCTC TCTGCAGAGC
    CAAGTGAATT ACAAGAAAGA CTTTGAACAC TCAAAGGCGC AGTTCCATCT GCCGTTGGAC
    ATGGTAACCC TGGTGCATGC CAAGAAGGCT CAGACCCTGG CCAGCGACCA GGACTACAGA
    CACCCACTCC CCCAGTACAC GTCCTTGGCT GAGGACCTGA GGCTCAGCTG CGCCAAGAGA
    GCTCACAAGT TGCAGAGTGA GAATTTGTAC CGGTCGGACC TGAACTTTAT GCGGGGTGTT
    GCCTGCGTCA TTCCAGGCAC GTTAGAGATT GAAGGGAGAA AGAGAGCATC TGAACTCATC
    AGTGAGAGTA AATATCGTCA GCATCCCCAG TCGCTCAAGT ACACAGCCGT GACAGACACA
    CCCAGCCTCA CTCATGTAAA ACTCAGCAAC CAGATTACAA ACGAGCGCCT CTATAAAGCA
    GCGGGGGAAG ATGCGAGACA CCAGTACACA ATGACCCTGG GCCTGCCCGA GTTTGTCCGA
    GCGAAAACAA ACGCGGCCAA TCTGAGTGAC GCAAAATACA AGGAGTCTTG GAGGAATCTC
    CATGCTCAAG GCTACAAGCT AACAATAGAT GCTCTGCCCT TCCAGGCGGC TCGAGTCTCT
    GGAGACATAG CCAGTGATTT TCTCTACAGA CATGACTTTG TGAAGGAGCG AGGACGGCTG
    ATCGGGGCCC AGAGCGTGAG TGACGACCCC CGGCTCCAGC ACTGTCAGCG GGTGGGCCAG
    CTGCAGAGCG AGCTTCAGTA CAGGAGGCAG GCGGCGGGCA GCAGAGCGCA GTGCCACCTG
    CCCATGGACA TGGTGCCACT GGTCCACGCC CGGAAGGCCC AGGCCCTGGC CAGCGACCTT
    GACTACAGGA CACAGTGCCA TGCGTTCACA GCGCTGCCCG AGGACCTGAG GATGGCCTGG
    GCCAAGAAGG CTCATGCTCT GCAGAGTGAG CTGCGCTACA AGTCGGACCT GATGGGCATG
    AAGGGGACAG GATGGCTGGC GCTGAGTTCC CCACAGATAG AAAGTGCAAA GAAGGCCGGA
    GAACTCATCA GTGAGACCAA ATACCGTGAA AAACCAGACA CCATCAAGTT TACCACAGTG
    GTTGACTCCC CGGACCTAGT TCATGCCAAG AACAGCTACA TGCATTGCAA TGAGCGCCTG
    TACAGGTCGG GGGATGCGGA ATCCCGACAC AGGTACACCC TGGTGCCCGA CCACCCCGAT
    TTCACCAGAG CCCGCCTCAA CGCCCTGAAT CTGAGCGACA AAGTCTACAG ACACTCCTGG
    GAGCAGACCC GGGCTGGCGG CTACGACTTC AGGCTGGATG CCATCCCATT CCAGACGGCA
    CGGGCATCGA GGGAAATCGC CAGTGATTTC CGGTACAAAG AGGCCTTCCT ACGGGACCGG
    GGCCTGCAGA TAGGGTACCG CAGCATCAAC GACGACCCGA GGACGAAGCA TTTCCTCAGC
    GTCGGCAGAC TCCAGAGTGA CAACGAGTAC AAGAAGGACT TCGCCAAGAG TCGGTCCCAG
    TTCCACAGCC GCCCAGACCA GCCCGGCTTC CTCCAGGCCA AGAGGAGCCA GCAGCTGGCC
    AGCGACGTGC ACTACAGGCA GCCCCTGCCC CAGCCCACCT GCGACCCGGA GCAGCTGGGC
    CTGAAACACG CGCAGAAGGC CCACCGGCTG CAGAGCGATG TCAAGTACAA ATCAGACTTG
    AACCTGACCA GAGGTATTGG CTGGACACCT CCTGGCTCCT ACAAAGTGGA AATGGCTCGG
    CGGGCTGCAG AATTGGCCAA CGCGAGGGGC CTGGGTCTCC AAGGGGCTTA CGGGGGGCCG
    GAGGCTGTGG AGCCCAGAGA TGATCAGAGC GGGTTTGTAA ACCCAGATGC CACCGAGATT
    CTGCACGTCA AAAGGAGGAA GGCCCCACTG TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225802.2
    CDS179..5374
    Translation

    Target ORF information:

    RefSeq Version XM_005225745.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225745.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCGTTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAAAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCACG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACAGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAACCAATA CAGGGCAGAT GTGAAGTGGA TGAAAGGGAC GGGCTGGGTC
    GCCACCGGGT CATTAAATGT GGAGCAGGCG AAGAAGGCAG GAGAACTCAT TAGCGAGAAG
    AAGTACCGGC AGCATCCAGA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCGGAGATG
    GTCCAGGCCA GAATTAGTTA TACCCAAGCA GTGGATAGGT TGTACCGAGA GCAAGGAGAA
    AACCTAAAGC ATCACTACAC GCAGACCACT GACCTCCCTG AAGTCCTGCT GGCCAAGCTG
    AATGCCATGA ATATCAGCGA GACACGTTAT AAGGAATCCT GGAGCAAACT TCGAGACGGT
    GGCTATAAGC TGAGACTGGA TGCCATTCCA TTCCAGGCAG CAAAGGCTTC TGGCGAAATC
    ATAAGTGATT ACAAATACAA AGAGGCATTT GAGAAAATGA AAGGGCAGAT GCTCGGCTCC
    CGGAGCTTGG AAGATGATAT CAGCCTTGCG CATTCAGTGT ACGCGAGCTC TCTGCAGAGC
    CAAGTGAATT ACAAGAAAGA CTTTGAACAC TCAAAGGCGC AGTTCCATCT GCCGTTGGAC
    ATGGTAACCC TGGTGCATGC CAAGAAGGCT CAGACCCTGG CCAGCGACCA GGACTACAGA
    CACCCACTCC CCCAGTACAC GTCCTTGGCT GAGGACCTGA GGCTCAGCTG CGCCAAGAGA
    GCTCACAAGT TGCAGAGTGA GAATTTGTAC CGGTCGGACC TGAACTTTAT GCGGGGTGTT
    GCCTGCGTCA TTCCAGGCAC GTTAGAGATT GAAGGGAGAA AGAGAGCATC TGAACTCATC
    AGTGAGAGTA AATATCGTCA GCATCCCCAG TCGCTCAAGT ACACAGCCGT GACAGACACA
    CCCAGCCTCA CTCATGTAAA ACTCAGCAAC CAGATTACAA ACGAGCGCCT CTATAAAGCA
    GCGGGGGAAG ATGCGAGACA CCAGTACACA ATGACCCTGG GCCTGCCCGA GTTTGTCCGA
    GCGAAAACAA ACGCGGCCAA TCTGAGTGAC GCAAAATACA AGGAGTCTTG GAGGAATCTC
    CATGCTCAAG GCTACAAGCT AACAATAGAT GCTCTGCCCT TCCAGGCGGC TCGAGTCTCT
    GGAGACATAG CCAGTGATTT TCTCTACAGA CATGACTTTG TGAAGGAGCG AGGACGGCTG
    ATCGGGGCCC AGAGCGTGAG TGACGACCCC CGGCTCCAGC ACTGTCAGCG GGTGGGCCAG
    CTGCAGAGCG AGCTTCAGTA CAGGAGGCAG GCGGCGGGCA GCAGAGCGCA GTGCCACCTG
    CCCATGGACA TGGTGCCACT GGTCCACGCC CGGAAGGCCC AGGCCCTGGC CAGCGACCTT
    GACTACAGGA CACAGTGCCA TGCGTTCACA GCGCTGCCCG AGGACCTGAG GATGGCCTGG
    GCCAAGAAGG CTCATGCTCT GCAGAGTGAG CTGCGCTACA AGTCGGACCT GATGGGCATG
    AAGGGGACAG GATGGCTGGC GCTGAGTTCC CCACAGATAG AAAGTGCAAA GAAGGCCGGA
    GAACTCATCA GTGAGACCAA ATACCGTGAA AAACCAGACA CCATCAAGTT TACCACAGTG
    GTTGACTCCC CGGACCTAGT TCATGCCAAG AACAGCTACA TGCATTGCAA TGAGCGCCTG
    TACAGGTCGG GGGATGCGGA ATCCCGACAC AGGTACACCC TGGTGCCCGA CCACCCCGAT
    TTCACCAGAG CCCGCCTCAA CGCCCTGAAT CTGAGCGACA AAGTCTACAG ACACTCCTGG
    GAGCAGACCC GGGCTGGCGG CTACGACTTC AGGCTGGATG CCATCCCATT CCAGACGGCA
    CGGGCATCGA GGGAAATCGC CAGTGATTTC CGGTACAAAG AGGCCTTCCT ACGGGACCGG
    GGCCTGCAGA TAGGGTACCG CAGCATCAAC GACGACCCGA GGACGAAGCA TTTCCTCAGC
    GTCGGCAGAC TCCAGAGTGA CAACGAGTAC AAGAAGGACT TCGCCAAGAG TCGGTCCCAG
    TTCCACAGCC GCCCAGACCA GCCCGGCTTC CTCCAGGCCA AGAGGAGCCA GCAGCTGGCC
    AGCGACGTGC ACTACAGGCA GCCCCTGCCC CAGCCCACCT GCGACCCGGA GCAGCTGGGC
    CTGAAACACG CGCAGAAGGC CCACCGGCTG CAGAGCGATG TCAAGTACAA ATCAGACTTG
    AACCTGACCA GAGGTATTGG CTGGACACCT CCTGGCTCCT ACAAAGTGGA AATGGCTCGG
    CGGGCTGCAG AATTGGCCAA CGCGAGGGGC CTGGGTCTCC AAGGGGCTTA CGGGGGGCCG
    GAGGCTGTGG AGCCCAGAGA TGATCAGAGC GGGTTTGTAA ACCCAGATGC CACCGAGATT
    CTGCACGTCA AAAGGAGGAA GGCCCCACTG TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230891
    Clone ID Related Accession (Same CDS sequence) XM_005225746.3
    Accession Version XM_005225746.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGAATAAAT ATAAAGAAAA CTACCAGAAC
    CGCATGAGAG GCCGCTATGA AGGAGTCGGG ATGGACAAAC GGATGCTCCA TGCTCTGAAA
    GTTGGAAGCC TGGCAAGCAA TATCGCCTAC AAGGCTGATT ACAAACACGA TGTTGTCGAC
    TACAACTACC CGGCCACTCT TACCCCTTCC TATCAAACAA CAGTGAAACT GGCTCCCTTG
    AAAGACGTCA ACTACAGGCA AAGCATCGAC AAGCTGAAGT ACAGCTCTGT GACCAACACG
    CCTCAGATTG TTCAAGCCAA AATCAATGCC CAGCAACTGA GTCATGTGAA TTACCGTGCT
    GACTATGAGA AGAACAAGTT GAATTATACG TTGCCCCAGG ATGTCCCTCA GCTGGTGAAG
    GCCAGAACCA ACGCGGAATT GTTTAGTGAG GTTAAGTACA GAGAAGGCTG GGAGAAGACA
    AAGGGGAAAG GATTTGAGAT GAAGCTGGAT GCCATGTCTC TGCTGGCTGC CAAAGCTTCC
    GGAGAGCTTG CAAGCAATAT TAAATACAAA GAGGAGTATG AAAAAGCAAA AGGCAAAGTC
    CTAGGAACCA CAGACTCGAG GCTGCTGCAC TCCCTTCAGG TTGCCAAGAT GAGCAGTGAG
    GTTGAATACA AGAAAGGCTT TGAAAAGAGT AAGACCCACT TTCACCTGCC CATGGACATG
    GTCAACATCA GGCATGCTAA GAAGGCCCAA GCTCTGGCCA GTGACCTGGA CTACAGGAAA
    AGACTGCACG AATACACTGT GCTGCCCGAA GATATGAAGA CTCTCTGGGC CAAGAAAGCC
    TACGGGCTCC AGAGCGAGCT GCAGTACAAG GCTGACCTGG CGTGGATGAA AGGGGTCAGG
    TGGCTGACTG AGGGCAGTCT CAACTTGGAA CAGGCCAAGA AGGCTGGACA GCTGGTCAGC
    GAGAAAAACT ACAGGCAGAG GGTGGATGAG CTGAAGTTCA CCAGTGTGGC CGACAGCTCC
    CAGATGGAGC ATGCCAAGAA GAGCCAGGAA CTGCAGAGCG GGGTGGCCTA CAAGGCAGAG
    CACGAGCAGT CCGTCCATCA GTACAGCATT AGCAAAGACG AGCCTCTCTT CCTACAGGCC
    CGAGCCAACG CTGCCAATCT CAGCGAGAAA CTGTATAAGA GCAGCTGGGA AAACCAGAAG
    GCGAAAGCGT TTGATCTGCG TCTTGACTCC TTGGCCTTCC TGGCAGCCAA AGCCAAGAGG
    GACCTGGCCA GTGAGGTGAA GTACAAAGAA GATTATGAAA AATCCAGAGG GAAGCTCATT
    GGAGCTCAGG GTGCCCAGGG GGATTCGCAA ATGAGCCACT CGCTGCAAAT GTCCAAGCTC
    CAGAGTGAAC TGGAATACAA AAAGGGCTTC GAGGACACCA AGTCTCAGTG CCACGTCCCC
    CTGGACATGA TCCACCTCGT GCATGCCCGC AAGGCTCAGC ACTTAGCCAC AGACATAGGC
    TACAAGACAG CATCCCATCA CTTTACAGCT TTGCCCACAG ACATGAAGGT GGAATGGGCC
    AAGAAGGCTT ACGGTTTACA GAGTGATAAC CAATACAGGG CAGATGTGAA GTGGATGAAA
    GGGACGGGCT GGGTCGCCAC CGGGTCATTA AATGTGGAGC AGGCGAAGAA GGCAGGAGAA
    CTCATTAGCG AGAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC ACTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GTAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC GGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ACGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCATGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCTCAT GCTCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225803.2
    CDS179..5269
    Translation

    Target ORF information:

    RefSeq Version XM_005225746.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225746.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGAATAAAT ATAAAGAAAA CTACCAGAAC
    CGCATGAGAG GCCGCTATGA AGGAGTCGGG ATGGACAAAC GGATGCTCCA TGCTCTGAAA
    GTTGGAAGCC TGGCAAGCAA TATCGCCTAC AAGGCTGATT ACAAACACGA TGTTGTCGAC
    TACAACTACC CGGCCACTCT TACCCCTTCC TATCAAACAA CAGTGAAACT GGCTCCCTTG
    AAAGACGTCA ACTACAGGCA AAGCATCGAC AAGCTGAAGT ACAGCTCTGT GACCAACACG
    CCTCAGATTG TTCAAGCCAA AATCAATGCC CAGCAACTGA GTCATGTGAA TTACCGTGCT
    GACTATGAGA AGAACAAGTT GAATTATACG TTGCCCCAGG ATGTCCCTCA GCTGGTGAAG
    GCCAGAACCA ACGCGGAATT GTTTAGTGAG GTTAAGTACA GAGAAGGCTG GGAGAAGACA
    AAGGGGAAAG GATTTGAGAT GAAGCTGGAT GCCATGTCTC TGCTGGCTGC CAAAGCTTCC
    GGAGAGCTTG CAAGCAATAT TAAATACAAA GAGGAGTATG AAAAAGCAAA AGGCAAAGTC
    CTAGGAACCA CAGACTCGAG GCTGCTGCAC TCCCTTCAGG TTGCCAAGAT GAGCAGTGAG
    GTTGAATACA AGAAAGGCTT TGAAAAGAGT AAGACCCACT TTCACCTGCC CATGGACATG
    GTCAACATCA GGCATGCTAA GAAGGCCCAA GCTCTGGCCA GTGACCTGGA CTACAGGAAA
    AGACTGCACG AATACACTGT GCTGCCCGAA GATATGAAGA CTCTCTGGGC CAAGAAAGCC
    TACGGGCTCC AGAGCGAGCT GCAGTACAAG GCTGACCTGG CGTGGATGAA AGGGGTCAGG
    TGGCTGACTG AGGGCAGTCT CAACTTGGAA CAGGCCAAGA AGGCTGGACA GCTGGTCAGC
    GAGAAAAACT ACAGGCAGAG GGTGGATGAG CTGAAGTTCA CCAGTGTGGC CGACAGCTCC
    CAGATGGAGC ATGCCAAGAA GAGCCAGGAA CTGCAGAGCG GGGTGGCCTA CAAGGCAGAG
    CACGAGCAGT CCGTCCATCA GTACAGCATT AGCAAAGACG AGCCTCTCTT CCTACAGGCC
    CGAGCCAACG CTGCCAATCT CAGCGAGAAA CTGTATAAGA GCAGCTGGGA AAACCAGAAG
    GCGAAAGCGT TTGATCTGCG TCTTGACTCC TTGGCCTTCC TGGCAGCCAA AGCCAAGAGG
    GACCTGGCCA GTGAGGTGAA GTACAAAGAA GATTATGAAA AATCCAGAGG GAAGCTCATT
    GGAGCTCAGG GTGCCCAGGG GGATTCGCAA ATGAGCCACT CGCTGCAAAT GTCCAAGCTC
    CAGAGTGAAC TGGAATACAA AAAGGGCTTC GAGGACACCA AGTCTCAGTG CCACGTCCCC
    CTGGACATGA TCCACCTCGT GCATGCCCGC AAGGCTCAGC ACTTAGCCAC AGACATAGGC
    TACAAGACAG CATCCCATCA CTTTACAGCT TTGCCCACAG ACATGAAGGT GGAATGGGCC
    AAGAAGGCTT ACGGTTTACA GAGTGATAAC CAATACAGGG CAGATGTGAA GTGGATGAAA
    GGGACGGGCT GGGTCGCCAC CGGGTCATTA AATGTGGAGC AGGCGAAGAA GGCAGGAGAA
    CTCATTAGCG AGAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC ACTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GTAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC GGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ACGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCATGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCTCAT GCTCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230892
    Clone ID Related Accession (Same CDS sequence) XM_010820038.3
    Accession Version XM_010820038.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCGTTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAAAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCACG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACAGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC ACTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GTAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC GGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ACGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCATGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCTCAT GCTCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818340.2
    CDS179..5269
    Translation

    Target ORF information:

    RefSeq Version XM_010820038.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820038.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCGTTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAAAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCACG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACAGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC ACTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GTAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC GGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ACGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCATGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCTCAT GCTCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230893
    Clone ID Related Accession (Same CDS sequence) XM_005225747.3
    Accession Version XM_005225747.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG AATAAATATA AAGAAAACTA CCAGAACCGC
    ATGAGAGGCC GCTATGAAGG AGTCGGGATG GACAAACGGA TGCTCCATGC TCTGAAAGTT
    GGAAGCCTGG CAAGCAATAT CGCCTACAAG GCTGATTACA AACACGATGT TGTCGACTAC
    AACTACCCGG CCACTCTTAC CCCTTCCTAT CAAACAACAG TGAAACTGGC TCCCTTGAAA
    GACGTCAACT ACAGGCAAAG CATCGACAAG CTGAAGTACA GCTCTGTGAC CAACACGCCT
    CAGATTGTTC AAGCCAAAAT CAATGCCCAG CAACTGAGTC ATGTGAATTA CCGTGCTGAC
    TATGAGAAGA ACAAGTTGAA TTATACGTTG CCCCAGGATG TCCCTCAGCT GGTGAAGGCC
    AGAACCAACG CGGAATTGTT TAGTGAGGTT AAGTACAGAG AAGGCTGGGA GAAGACAAAG
    GGGAAAGGAT TTGAGATGAA GCTGGATGCC ATGTCTCTGC TGGCTGCCAA AGCTTCCGGA
    GAGCTTGCAA GCAATATTAA ATACAAAGAG GAGTATGAAA AAGCAAAAGG CAAAGTCCTA
    GGAACCACAG ACTCGAGGCT GCTGCACTCC CTTCAGGTTG CCAAGATGAG CAGTGAGGTT
    GAATACAAGA AAGGCTTTGA AAAGAGTAAG ACCCACTTTC ACCTGCCCAT GGACATGGTC
    AACATCAGGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAGA
    CTGCACGAAT ACACTGTGCT GCCCGAAGAT ATGAAGACTC TCTGGGCCAA GAAAGCCTAC
    GGGCTCCAGA GCGAGCTGCA GTACAAGGCT GACCTGGCGT GGATGAAAGG GGTCAGGTGG
    CTGACTGAGG GCAGTCTCAA CTTGGAACAG GCCAAGAAGG CTGGACAGCT GGTCAGCGAG
    AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGGCCGA CAGCTCCCAG
    ATGGAGCATG CCAAGAAGAG CCAGGAACTG CAGAGCGGGG TGGCCTACAA GGCAGAGCAC
    GAGCAGTCCG TCCATCAGTA CAGCATTAGC AAAGACGAGC CTCTCTTCCT ACAGGCCCGA
    GCCAACGCTG CCAATCTCAG CGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCG
    AAAGCGTTTG ATCTGCGTCT TGACTCCTTG GCCTTCCTGG CAGCCAAAGC CAAGAGGGAC
    CTGGCCAGTG AGGTGAAGTA CAAAGAAGAT TATGAAAAAT CCAGAGGGAA GCTCATTGGA
    GCTCAGGGTG CCCAGGGGGA TTCGCAAATG AGCCACTCGC TGCAAATGTC CAAGCTCCAG
    AGTGAACTGG AATACAAAAA GGGCTTCGAG GACACCAAGT CTCAGTGCCA CGTCCCCCTG
    GACATGATCC ACCTCGTGCA TGCCCGCAAG GCTCAGCACT TAGCCACAGA CATAGGCTAC
    AAGACAGCAT CCCATCACTT TACAGCTTTG CCCACAGACA TGAAGGTGGA ATGGGCCAAG
    AAGGCTTACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGG
    ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC
    ATTAGCGAGA AGAAGTACCG GCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC
    ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTGTACCGA
    GAGCAAGGAG AAAACCTAAA GCATCACTAC ACGCAGACCA CTGACCTCCC TGAAGTCCTG
    CTGGCCAAGC TGAATGCCAT GAATATCAGC GAGACACGTT ATAAGGAATC CTGGAGCAAA
    CTTCGAGACG GTGGCTATAA GCTGAGACTG GATGCCATTC CATTCCAGGC AGCAAAGGCT
    TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG
    ATGCTCGGCT CCCGGAGCTT GGAAGATGAT ATCAGCCTTG CGCATTCAGT GTACGCGAGC
    TCTCTGCAGA GCCAAGTGAA TTACAAGAAA GACTTTGAAC ACTCAAAGGC GCAGTTCCAT
    CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGACCCT GGCCAGCGAC
    CAGGACTACA GACACCCACT CCCCCAGTAC ACGTCCTTGG CTGAGGACCT GAGGCTCAGC
    TGCGCCAAGA GAGCTCACAA GTTGCAGAGT GAGAATTTGT ACCGGTCGGA CCTGAACTTT
    ATGCGGGGTG TTGCCTGCGT CATTCCAGGC ACGTTAGAGA TTGAAGGGAG AAAGAGAGCA
    TCTGAACTCA TCAGTGAGAG TAAATATCGT CAGCATCCCC AGTCGCTCAA GTACACAGCC
    GTGACAGACA CACCCAGCCT CACTCATGTA AAACTCAGCA ACCAGATTAC AAACGAGCGC
    CTCTATAAAG CAGCGGGGGA AGATGCGAGA CACCAGTACA CAATGACCCT GGGCCTGCCC
    GAGTTTGTCC GAGCGAAAAC AAACGCGGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT
    TGGAGGAATC TCCATGCTCA AGGCTACAAG CTAACAATAG ATGCTCTGCC CTTCCAGGCG
    GCTCGAGTCT CTGGAGACAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG
    CGAGGACGGC TGATCGGGGC CCAGAGCGTG AGTGACGACC CCCGGCTCCA GCACTGTCAG
    CGGGTGGGCC AGCTGCAGAG CGAGCTTCAG TACAGGAGGC AGGCGGCGGG CAGCAGAGCG
    CAGTGCCACC TGCCCATGGA CATGGTGCCA CTGGTCCACG CCCGGAAGGC CCAGGCCCTG
    GCCAGCGACC TTGACTACAG GACACAGTGC CATGCGTTCA CAGCGCTGCC CGAGGACCTG
    AGGATGGCCT GGGCCAAGAA GGCTCATGCT CTGCAGAGTG AGCTGCGCTA CAAGTCGGAC
    CTGATGGGCA TGAAGGGGAC AGGATGGCTG GCGCTGAGTT CCCCACAGAT AGAAAGTGCA
    AAGAAGGCCG GAGAACTCAT CAGTGAGACC AAATACCGTG AAAAACCAGA CACCATCAAG
    TTTACCACAG TGGTTGACTC CCCGGACCTA GTTCATGCCA AGAACAGCTA CATGCATTGC
    AATGAGCGCC TGTACAGGTC GGGGGATGCG GAATCCCGAC ACAGGTACAC CCTGGTGCCC
    GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCCCTGA ATCTGAGCGA CAAAGTCTAC
    AGACACTCCT GGGAGCAGAC CCGGGCTGGC GGCTACGACT TCAGGCTGGA TGCCATCCCA
    TTCCAGACGG CACGGGCATC GAGGGAAATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC
    CTACGGGACC GGGGCCTGCA GATAGGGTAC CGCAGCATCA ACGACGACCC GAGGACGAAG
    CATTTCCTCA GCGTCGGCAG ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTCGCCAAG
    AGTCGGTCCC AGTTCCACAG CCGCCCAGAC CAGCCCGGCT TCCTCCAGGC CAAGAGGAGC
    CAGCAGCTGG CCAGCGACGT GCACTACAGG CAGCCCCTGC CCCAGCCCAC CTGCGACCCG
    GAGCAGCTGG GCCTGAAACA CGCGCAGAAG GCCCACCGGC TGCAGAGCGA TGTCAAGTAC
    AAATCAGACT TGAACCTGAC CAGAGGTATT GGCTGGACAC CTCCTGGCTC CTACAAAGTG
    GAAATGGCTC GGCGGGCTGC AGAATTGGCC AACGCGAGGG GCCTGGGTCT CCAAGGGGCT
    TACGGGGGGC CGGAGGCTGT GGAGCCCAGA GATGATCAGA GCGGGTTTGT AAACCCAGAT
    GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCCCCAC TGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225804.2
    CDS337..5424
    Translation

    Target ORF information:

    RefSeq Version XM_005225747.3
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225747.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG AATAAATATA AAGAAAACTA CCAGAACCGC
    ATGAGAGGCC GCTATGAAGG AGTCGGGATG GACAAACGGA TGCTCCATGC TCTGAAAGTT
    GGAAGCCTGG CAAGCAATAT CGCCTACAAG GCTGATTACA AACACGATGT TGTCGACTAC
    AACTACCCGG CCACTCTTAC CCCTTCCTAT CAAACAACAG TGAAACTGGC TCCCTTGAAA
    GACGTCAACT ACAGGCAAAG CATCGACAAG CTGAAGTACA GCTCTGTGAC CAACACGCCT
    CAGATTGTTC AAGCCAAAAT CAATGCCCAG CAACTGAGTC ATGTGAATTA CCGTGCTGAC
    TATGAGAAGA ACAAGTTGAA TTATACGTTG CCCCAGGATG TCCCTCAGCT GGTGAAGGCC
    AGAACCAACG CGGAATTGTT TAGTGAGGTT AAGTACAGAG AAGGCTGGGA GAAGACAAAG
    GGGAAAGGAT TTGAGATGAA GCTGGATGCC ATGTCTCTGC TGGCTGCCAA AGCTTCCGGA
    GAGCTTGCAA GCAATATTAA ATACAAAGAG GAGTATGAAA AAGCAAAAGG CAAAGTCCTA
    GGAACCACAG ACTCGAGGCT GCTGCACTCC CTTCAGGTTG CCAAGATGAG CAGTGAGGTT
    GAATACAAGA AAGGCTTTGA AAAGAGTAAG ACCCACTTTC ACCTGCCCAT GGACATGGTC
    AACATCAGGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAGA
    CTGCACGAAT ACACTGTGCT GCCCGAAGAT ATGAAGACTC TCTGGGCCAA GAAAGCCTAC
    GGGCTCCAGA GCGAGCTGCA GTACAAGGCT GACCTGGCGT GGATGAAAGG GGTCAGGTGG
    CTGACTGAGG GCAGTCTCAA CTTGGAACAG GCCAAGAAGG CTGGACAGCT GGTCAGCGAG
    AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGGCCGA CAGCTCCCAG
    ATGGAGCATG CCAAGAAGAG CCAGGAACTG CAGAGCGGGG TGGCCTACAA GGCAGAGCAC
    GAGCAGTCCG TCCATCAGTA CAGCATTAGC AAAGACGAGC CTCTCTTCCT ACAGGCCCGA
    GCCAACGCTG CCAATCTCAG CGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCG
    AAAGCGTTTG ATCTGCGTCT TGACTCCTTG GCCTTCCTGG CAGCCAAAGC CAAGAGGGAC
    CTGGCCAGTG AGGTGAAGTA CAAAGAAGAT TATGAAAAAT CCAGAGGGAA GCTCATTGGA
    GCTCAGGGTG CCCAGGGGGA TTCGCAAATG AGCCACTCGC TGCAAATGTC CAAGCTCCAG
    AGTGAACTGG AATACAAAAA GGGCTTCGAG GACACCAAGT CTCAGTGCCA CGTCCCCCTG
    GACATGATCC ACCTCGTGCA TGCCCGCAAG GCTCAGCACT TAGCCACAGA CATAGGCTAC
    AAGACAGCAT CCCATCACTT TACAGCTTTG CCCACAGACA TGAAGGTGGA ATGGGCCAAG
    AAGGCTTACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGG
    ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC
    ATTAGCGAGA AGAAGTACCG GCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC
    ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTGTACCGA
    GAGCAAGGAG AAAACCTAAA GCATCACTAC ACGCAGACCA CTGACCTCCC TGAAGTCCTG
    CTGGCCAAGC TGAATGCCAT GAATATCAGC GAGACACGTT ATAAGGAATC CTGGAGCAAA
    CTTCGAGACG GTGGCTATAA GCTGAGACTG GATGCCATTC CATTCCAGGC AGCAAAGGCT
    TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG
    ATGCTCGGCT CCCGGAGCTT GGAAGATGAT ATCAGCCTTG CGCATTCAGT GTACGCGAGC
    TCTCTGCAGA GCCAAGTGAA TTACAAGAAA GACTTTGAAC ACTCAAAGGC GCAGTTCCAT
    CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGACCCT GGCCAGCGAC
    CAGGACTACA GACACCCACT CCCCCAGTAC ACGTCCTTGG CTGAGGACCT GAGGCTCAGC
    TGCGCCAAGA GAGCTCACAA GTTGCAGAGT GAGAATTTGT ACCGGTCGGA CCTGAACTTT
    ATGCGGGGTG TTGCCTGCGT CATTCCAGGC ACGTTAGAGA TTGAAGGGAG AAAGAGAGCA
    TCTGAACTCA TCAGTGAGAG TAAATATCGT CAGCATCCCC AGTCGCTCAA GTACACAGCC
    GTGACAGACA CACCCAGCCT CACTCATGTA AAACTCAGCA ACCAGATTAC AAACGAGCGC
    CTCTATAAAG CAGCGGGGGA AGATGCGAGA CACCAGTACA CAATGACCCT GGGCCTGCCC
    GAGTTTGTCC GAGCGAAAAC AAACGCGGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT
    TGGAGGAATC TCCATGCTCA AGGCTACAAG CTAACAATAG ATGCTCTGCC CTTCCAGGCG
    GCTCGAGTCT CTGGAGACAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG
    CGAGGACGGC TGATCGGGGC CCAGAGCGTG AGTGACGACC CCCGGCTCCA GCACTGTCAG
    CGGGTGGGCC AGCTGCAGAG CGAGCTTCAG TACAGGAGGC AGGCGGCGGG CAGCAGAGCG
    CAGTGCCACC TGCCCATGGA CATGGTGCCA CTGGTCCACG CCCGGAAGGC CCAGGCCCTG
    GCCAGCGACC TTGACTACAG GACACAGTGC CATGCGTTCA CAGCGCTGCC CGAGGACCTG
    AGGATGGCCT GGGCCAAGAA GGCTCATGCT CTGCAGAGTG AGCTGCGCTA CAAGTCGGAC
    CTGATGGGCA TGAAGGGGAC AGGATGGCTG GCGCTGAGTT CCCCACAGAT AGAAAGTGCA
    AAGAAGGCCG GAGAACTCAT CAGTGAGACC AAATACCGTG AAAAACCAGA CACCATCAAG
    TTTACCACAG TGGTTGACTC CCCGGACCTA GTTCATGCCA AGAACAGCTA CATGCATTGC
    AATGAGCGCC TGTACAGGTC GGGGGATGCG GAATCCCGAC ACAGGTACAC CCTGGTGCCC
    GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCCCTGA ATCTGAGCGA CAAAGTCTAC
    AGACACTCCT GGGAGCAGAC CCGGGCTGGC GGCTACGACT TCAGGCTGGA TGCCATCCCA
    TTCCAGACGG CACGGGCATC GAGGGAAATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC
    CTACGGGACC GGGGCCTGCA GATAGGGTAC CGCAGCATCA ACGACGACCC GAGGACGAAG
    CATTTCCTCA GCGTCGGCAG ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTCGCCAAG
    AGTCGGTCCC AGTTCCACAG CCGCCCAGAC CAGCCCGGCT TCCTCCAGGC CAAGAGGAGC
    CAGCAGCTGG CCAGCGACGT GCACTACAGG CAGCCCCTGC CCCAGCCCAC CTGCGACCCG
    GAGCAGCTGG GCCTGAAACA CGCGCAGAAG GCCCACCGGC TGCAGAGCGA TGTCAAGTAC
    AAATCAGACT TGAACCTGAC CAGAGGTATT GGCTGGACAC CTCCTGGCTC CTACAAAGTG
    GAAATGGCTC GGCGGGCTGC AGAATTGGCC AACGCGAGGG GCCTGGGTCT CCAAGGGGCT
    TACGGGGGGC CGGAGGCTGT GGAGCCCAGA GATGATCAGA GCGGGTTTGT AAACCCAGAT
    GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCCCCAC TGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230894
    Clone ID Related Accession (Same CDS sequence) XM_024985666.1
    Accession Version XM_024985666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG GTGGAGTATA AGAAGGATCT GGAAAGTAGT
    AAAGGTCATA GTATCAACTA CTGTGAAACG CCTCAATTCA GGAATGTGTG CAAGATTTCA
    AAATTCACCA GCGATAATAA ATATAAAGAA AACTACCAGA ACCGCATGAG AGGCCGCTAT
    GAAGGAGTCG GGATGGACAA ACGGATGCTC CATGCTCTGA AAGTTGGAAG CCTGGCAAGC
    AATATCGCCT ACAAGGCTGA TTACAAACAC GATGTTGTCG ACTACAACTA CCCGGCCACT
    CTTACCCCTT CCTATCAAAC AACAGTGAAA CTGGCTCCCT TGAAAGACGT CAACTACAGG
    CAAAGCATCG ACAAGCTGAA GTACAGCTCT GTGACCAACA CGCCTCAGAT TGTTCAAGCC
    AAAATCAATG CCCAGCAACT GAGTCATGTG AATTACCGTG CTGACTATGA GAAGAACAAG
    TTGAATTATA CGTTGCCCCA GGATGTCCCT CAGCTGGTGA AGGCCAGAAC CAACGCGGAA
    TTGTTTAGTG AGGTTAAGTA CAGAGAAGGC TGGGAGAAGA CAAAGGGGAA AGGATTTGAG
    ATGAAGCTGG ATGCCATGTC TCTGCTGGCT GCCAAAGCTT CCGGAGAGCT TGCAAGCAAT
    ATTAAATACA AAGAGGAGTA TGAAAAAGCA AAAGGCAAAG TCCTAGGAAC CACAGACTCG
    AGGCTGCTGC ACTCCCTTCA GGTTGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC
    TTTGAAAAGA GTAAGACCCA CTTTCACCTG CCCATGGACA TGGTCAACAT CAGGCATGCT
    AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAGACTGCA CGAATACACT
    GTGCTGCCCG AAGATATGAA GACTCTCTGG GCCAAGAAAG CCTACGGGCT CCAGAGCGAG
    CTGCAGTACA AGGCTGACCT GGCGTGGATG AAAGGGGTCA GGTGGCTGAC TGAGGGCAGT
    CTCAACTTGG AACAGGCCAA GAAGGCTGGA CAGCTGGTCA GCGAGAAAAA CTACAGGCAG
    AGGGTGGATG AGCTGAAGTT CACCAGTGTG GCCGACAGCT CCCAGATGGA GCATGCCAAG
    AAGAGCCAGG AACTGCAGAG CGGGGTGGCC TACAAGGCAG AGCACGAGCA GTCCGTCCAT
    CAGTACAGCA TTAGCAAAGA CGAGCCTCTC TTCCTACAGG CCCGAGCCAA CGCTGCCAAT
    CTCAGCGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCGAAAGC GTTTGATCTG
    CGTCTTGACT CCTTGGCCTT CCTGGCAGCC AAAGCCAAGA GGGACCTGGC CAGTGAGGTG
    AAGTACAAAG AAGATTATGA AAAATCCAGA GGGAAGCTCA TTGGAGCTCA GGGTGCCCAG
    GGGGATTCGC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TCCAGAGTGA ACTGGAATAC
    AAAAAGGGCT TCGAGGACAC CAAGTCTCAG TGCCACGTCC CCCTGGACAT GATCCACCTC
    GTGCATGCCC GCAAGGCTCA GCACTTAGCC ACAGACATAG GCTACAAGAC AGCATCCCAT
    CACTTTACAG CTTTGCCCAC AGACATGAAG GTGGAATGGG CCAAGAAGGC TTACGGTTTA
    CAGAGTGATA AGAAGTACCG GCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC
    ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTGTACCGA
    GAGCAAGGAG AAAACCTAAA GCATCACTAC ACGCAGACCA CTGACCTCCC TGAAGTCCTG
    CTGGCCAAGC TGAATGCCAT GAATATCAGC GAGACACGTT ATAAGGAATC CTGGAGCAAA
    CTTCGAGACG GTGGCTATAA GCTGAGACTG GATGCCATTC CATTCCAGGC AGCAAAGGCT
    TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG
    ATGCTCGGCT CCCGGAGCTT GGAAGATGAT ATCAGCCTTG CGCATTCAGT GTACGCGAGC
    TCTCTGCAGA GCCAAGTGAA TTACAAGAAA GACTTTGAAC ACTCAAAGGC GCAGTTCCAT
    CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGACCCT GGCCAGCGAC
    CAGGACTACA GACACCCACT CCCCCAGTAC ACGTCCTTGG CTGAGGACCT GAGGCTCAGC
    TGCGCCAAGA GAGCTCACAA GTTGCAGAGT GAGAATTTGT ACCGGTCGGA CCTGAACTTT
    ATGCGGGGTG TTGCCTGCGT CATTCCAGGC ACGTTAGAGA TTGAAGGGAG AAAGAGAGCA
    TCTGAACTCA TCAGTGAGAG TAAATATCGT CAGCATCCCC AGTCGCTCAA GTACACAGCC
    GTGACAGACA CACCCAGCCT CACTCATGTA AAACTCAGCA ACCAGATTAC AAACGAGCGC
    CTCTATAAAG CAGCGGGGGA AGATGCGAGA CACCAGTACA CAATGACCCT GGGCCTGCCC
    GAGTTTGTCC GAGCGAAAAC AAACGCGGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT
    TGGAGGAATC TCCATGCTCA AGGCTACAAG CTAACAATAG ATGCTCTGCC CTTCCAGGCG
    GCTCGAGTCT CTGGAGACAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG
    CGAGGACGGC TGATCGGGGC CCAGAGCGTG AGTGACGACC CCCGGCTCCA GCACTGTCAG
    CGGGTGGGCC AGCTGCAGAG CGAGCTTCAG TACAGGAGGC AGGCGGCGGG CAGCAGAGCG
    CAGTGCCACC TGCCCATGGA CATGGTGCCA CTGGTCCACG CCCGGAAGGC CCAGGCCCTG
    GCCAGCGACC TTGACTACAG GACACAGTGC CATGCGTTCA CAGCGCTGCC CGAGGACCTG
    AGGATGGCCT GGGCCAAGAA GGCTCATGCT CTGCAGAGTG AGCTGCGCTA CAAGTCGGAC
    CTGATGGGCA TGAAGGGGAC AGGATGGCTG GCGCTGAGTT CCCCACAGAT AGAAAGTGCA
    AAGAAGGCCG GAGAACTCAT CAGTGAGACC AAATACCGTG AAAAACCAGA CACCATCAAG
    TTTACCACAG TGGTTGACTC CCCGGACCTA GTTCATGCCA AGAACAGCTA CATGCATTGC
    AATGAGCGCC TGTACAGGTC GGGGGATGCG GAATCCCGAC ACAGGTACAC CCTGGTGCCC
    GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCCCTGA ATCTGAGCGA CAAAGTCTAC
    AGACACTCCT GGGAGCAGAC CCGGGCTGGC GGCTACGACT TCAGGCTGGA TGCCATCCCA
    TTCCAGACGG CACGGGCATC GAGGGAAATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC
    CTACGGGACC GGGGCCTGCA GATAGGGTAC CGCAGCATCA ACGACGACCC GAGGACGAAG
    CATTTCCTCA GCGTCGGCAG ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTCGCCAAG
    AGTCGGTCCC AGTTCCACAG CCGCCCAGAC CAGCCCGGCT TCCTCCAGGC CAAGAGGAGC
    CAGCAGCTGG CCAGCGACGT GCACTACAGG CAGCCCCTGC CCCAGCCCAC CTGCGACCCG
    GAGCAGCTGG GCCTGAAACA CGCGCAGAAG GCCCACCGGC TGCAGAGCGA TGTCAAGTAC
    AAATCAGACT TGAACCTGAC CAGAGGTATT GGCTGGACAC CTCCTGGCTC CTACAAAGTG
    GAAATGGCTC GGCGGGCTGC AGAATTGGCC AACGCGAGGG GCCTGGGTCT CCAAGGGGCT
    TACGGGGGGC CGGAGGCTGT GGAGCCCAGA GATGATCAGA GCGGGTTTGT AAACCCAGAT
    GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCCCCAC TGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841434.1
    CDS179..5266
    Translation

    Target ORF information:

    RefSeq Version XM_024985666.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG GTGGAGTATA AGAAGGATCT GGAAAGTAGT
    AAAGGTCATA GTATCAACTA CTGTGAAACG CCTCAATTCA GGAATGTGTG CAAGATTTCA
    AAATTCACCA GCGATAATAA ATATAAAGAA AACTACCAGA ACCGCATGAG AGGCCGCTAT
    GAAGGAGTCG GGATGGACAA ACGGATGCTC CATGCTCTGA AAGTTGGAAG CCTGGCAAGC
    AATATCGCCT ACAAGGCTGA TTACAAACAC GATGTTGTCG ACTACAACTA CCCGGCCACT
    CTTACCCCTT CCTATCAAAC AACAGTGAAA CTGGCTCCCT TGAAAGACGT CAACTACAGG
    CAAAGCATCG ACAAGCTGAA GTACAGCTCT GTGACCAACA CGCCTCAGAT TGTTCAAGCC
    AAAATCAATG CCCAGCAACT GAGTCATGTG AATTACCGTG CTGACTATGA GAAGAACAAG
    TTGAATTATA CGTTGCCCCA GGATGTCCCT CAGCTGGTGA AGGCCAGAAC CAACGCGGAA
    TTGTTTAGTG AGGTTAAGTA CAGAGAAGGC TGGGAGAAGA CAAAGGGGAA AGGATTTGAG
    ATGAAGCTGG ATGCCATGTC TCTGCTGGCT GCCAAAGCTT CCGGAGAGCT TGCAAGCAAT
    ATTAAATACA AAGAGGAGTA TGAAAAAGCA AAAGGCAAAG TCCTAGGAAC CACAGACTCG
    AGGCTGCTGC ACTCCCTTCA GGTTGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC
    TTTGAAAAGA GTAAGACCCA CTTTCACCTG CCCATGGACA TGGTCAACAT CAGGCATGCT
    AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAGACTGCA CGAATACACT
    GTGCTGCCCG AAGATATGAA GACTCTCTGG GCCAAGAAAG CCTACGGGCT CCAGAGCGAG
    CTGCAGTACA AGGCTGACCT GGCGTGGATG AAAGGGGTCA GGTGGCTGAC TGAGGGCAGT
    CTCAACTTGG AACAGGCCAA GAAGGCTGGA CAGCTGGTCA GCGAGAAAAA CTACAGGCAG
    AGGGTGGATG AGCTGAAGTT CACCAGTGTG GCCGACAGCT CCCAGATGGA GCATGCCAAG
    AAGAGCCAGG AACTGCAGAG CGGGGTGGCC TACAAGGCAG AGCACGAGCA GTCCGTCCAT
    CAGTACAGCA TTAGCAAAGA CGAGCCTCTC TTCCTACAGG CCCGAGCCAA CGCTGCCAAT
    CTCAGCGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCGAAAGC GTTTGATCTG
    CGTCTTGACT CCTTGGCCTT CCTGGCAGCC AAAGCCAAGA GGGACCTGGC CAGTGAGGTG
    AAGTACAAAG AAGATTATGA AAAATCCAGA GGGAAGCTCA TTGGAGCTCA GGGTGCCCAG
    GGGGATTCGC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TCCAGAGTGA ACTGGAATAC
    AAAAAGGGCT TCGAGGACAC CAAGTCTCAG TGCCACGTCC CCCTGGACAT GATCCACCTC
    GTGCATGCCC GCAAGGCTCA GCACTTAGCC ACAGACATAG GCTACAAGAC AGCATCCCAT
    CACTTTACAG CTTTGCCCAC AGACATGAAG GTGGAATGGG CCAAGAAGGC TTACGGTTTA
    CAGAGTGATA AGAAGTACCG GCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC
    ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTGTACCGA
    GAGCAAGGAG AAAACCTAAA GCATCACTAC ACGCAGACCA CTGACCTCCC TGAAGTCCTG
    CTGGCCAAGC TGAATGCCAT GAATATCAGC GAGACACGTT ATAAGGAATC CTGGAGCAAA
    CTTCGAGACG GTGGCTATAA GCTGAGACTG GATGCCATTC CATTCCAGGC AGCAAAGGCT
    TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG
    ATGCTCGGCT CCCGGAGCTT GGAAGATGAT ATCAGCCTTG CGCATTCAGT GTACGCGAGC
    TCTCTGCAGA GCCAAGTGAA TTACAAGAAA GACTTTGAAC ACTCAAAGGC GCAGTTCCAT
    CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGACCCT GGCCAGCGAC
    CAGGACTACA GACACCCACT CCCCCAGTAC ACGTCCTTGG CTGAGGACCT GAGGCTCAGC
    TGCGCCAAGA GAGCTCACAA GTTGCAGAGT GAGAATTTGT ACCGGTCGGA CCTGAACTTT
    ATGCGGGGTG TTGCCTGCGT CATTCCAGGC ACGTTAGAGA TTGAAGGGAG AAAGAGAGCA
    TCTGAACTCA TCAGTGAGAG TAAATATCGT CAGCATCCCC AGTCGCTCAA GTACACAGCC
    GTGACAGACA CACCCAGCCT CACTCATGTA AAACTCAGCA ACCAGATTAC AAACGAGCGC
    CTCTATAAAG CAGCGGGGGA AGATGCGAGA CACCAGTACA CAATGACCCT GGGCCTGCCC
    GAGTTTGTCC GAGCGAAAAC AAACGCGGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT
    TGGAGGAATC TCCATGCTCA AGGCTACAAG CTAACAATAG ATGCTCTGCC CTTCCAGGCG
    GCTCGAGTCT CTGGAGACAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG
    CGAGGACGGC TGATCGGGGC CCAGAGCGTG AGTGACGACC CCCGGCTCCA GCACTGTCAG
    CGGGTGGGCC AGCTGCAGAG CGAGCTTCAG TACAGGAGGC AGGCGGCGGG CAGCAGAGCG
    CAGTGCCACC TGCCCATGGA CATGGTGCCA CTGGTCCACG CCCGGAAGGC CCAGGCCCTG
    GCCAGCGACC TTGACTACAG GACACAGTGC CATGCGTTCA CAGCGCTGCC CGAGGACCTG
    AGGATGGCCT GGGCCAAGAA GGCTCATGCT CTGCAGAGTG AGCTGCGCTA CAAGTCGGAC
    CTGATGGGCA TGAAGGGGAC AGGATGGCTG GCGCTGAGTT CCCCACAGAT AGAAAGTGCA
    AAGAAGGCCG GAGAACTCAT CAGTGAGACC AAATACCGTG AAAAACCAGA CACCATCAAG
    TTTACCACAG TGGTTGACTC CCCGGACCTA GTTCATGCCA AGAACAGCTA CATGCATTGC
    AATGAGCGCC TGTACAGGTC GGGGGATGCG GAATCCCGAC ACAGGTACAC CCTGGTGCCC
    GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCCCTGA ATCTGAGCGA CAAAGTCTAC
    AGACACTCCT GGGAGCAGAC CCGGGCTGGC GGCTACGACT TCAGGCTGGA TGCCATCCCA
    TTCCAGACGG CACGGGCATC GAGGGAAATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC
    CTACGGGACC GGGGCCTGCA GATAGGGTAC CGCAGCATCA ACGACGACCC GAGGACGAAG
    CATTTCCTCA GCGTCGGCAG ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTCGCCAAG
    AGTCGGTCCC AGTTCCACAG CCGCCCAGAC CAGCCCGGCT TCCTCCAGGC CAAGAGGAGC
    CAGCAGCTGG CCAGCGACGT GCACTACAGG CAGCCCCTGC CCCAGCCCAC CTGCGACCCG
    GAGCAGCTGG GCCTGAAACA CGCGCAGAAG GCCCACCGGC TGCAGAGCGA TGTCAAGTAC
    AAATCAGACT TGAACCTGAC CAGAGGTATT GGCTGGACAC CTCCTGGCTC CTACAAAGTG
    GAAATGGCTC GGCGGGCTGC AGAATTGGCC AACGCGAGGG GCCTGGGTCT CCAAGGGGCT
    TACGGGGGGC CGGAGGCTGT GGAGCCCAGA GATGATCAGA GCGGGTTTGT AAACCCAGAT
    GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCCCCAC TGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230895
    Clone ID Related Accession (Same CDS sequence) XM_024985667.1
    Accession Version XM_024985667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG AATAAATATA AAGAAAACTA CCAGAACCGC
    ATGAGAGGCC GCTATGAAGG AGTCGGGATG GACAAACGGA TGCTCCATGC TCTGAAAGTT
    GGAAGCCTGG CAAGCAATAT CGCCTACAAG GCTGATTACA AACACGATGT TGTCGACTAC
    AACTACCCGG CCACTCTTAC CCCTTCCTAT CAAACAACAG TGAAACTGGC TCCCTTGAAA
    GACGTCAACT ACAGGCAAAG CATCGACAAG CTGAAGTACA GCTCTGTGAC CAACACGCCT
    CAGATTGTTC AAGCCAAAAT CAATGCCCAG CAACTGAGTC ATGTGAATTA CCGTGCTGAC
    TATGAGAAGA ACAAGTTGAA TTATACGTTG CCCCAGGATG TCCCTCAGCT GGTGAAGGCC
    AGAACCAACG CGGAATTGTT TAGTGAGGTT AAGTACAGAG AAGGCTGGGA GAAGACAAAG
    GGGAAAGGAT TTGAGATGAA GCTGGATGCC ATGTCTCTGC TGGCTGCCAA AGCTTCCGGA
    GAGCTTGCAA GCAATATTAA ATACAAAGAG GAGTATGAAA AAGCAAAAGG CAAAGTCCTA
    GGAACCACAG ACTCGAGGCT GCTGCACTCC CTTCAGGTTG CCAAGATGAG CAGTGAGGTT
    GAATACAAGA AAGGCTTTGA AAAGAGTAAG ACCCACTTTC ACCTGCCCAT GGACATGGTC
    AACATCAGGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAGA
    CTGCACGAAT ACACTGTGCT GCCCGAAGAT ATGAAGACTC TCTGGGCCAA GAAAGCCTAC
    GGGCTCCAGA GCGAGCTGCA GTACAAGGCT GACCTGGCGT GGATGAAAGG GGTCAGGTGG
    CTGACTGAGG GCAGTCTCAA CTTGGAACAG GCCAAGAAGG CTGGACAGCT GGTCAGCGAG
    AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGGCCGA CAGCTCCCAG
    ATGGAGCATG CCAAGAAGAG CCAGGAACTG CAGAGCGGGG TGGCCTACAA GGCAGAGCAC
    GAGCAGTCCG TCCATCAGTA CAGCATTAGC AAAGACGAGC CTCTCTTCCT ACAGGCCCGA
    GCCAACGCTG CCAATCTCAG CGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCG
    AAAGCGTTTG ATCTGCGTCT TGACTCCTTG GCCTTCCTGG CAGCCAAAGC CAAGAGGGAC
    CTGGCCAGTG AGGTGAAGTA CAAAGAAGAT TATGAAAAAT CCAGAGGGAA GCTCATTGGA
    GCTCAGGGTG CCCAGGGGGA TTCGCAAATG AGCCACTCGC TGCAAATGTC CAAGCTCCAG
    AGTGAACTGG AATACAAAAA GGGCTTCGAG GACACCAAGT CTCAGTGCCA CGTCCCCCTG
    GACATGATCC ACCTCGTGCA TGCCCGCAAG GCTCAGCACT TAGCCACAGA CATAGGCTAC
    AAGACAGCAT CCCATCACTT TACAGCTTTG CCCACAGACA TGAAGGTGGA ATGGGCCAAG
    AAGGCTTACG GTTTACAGAG TGATAAGAAG TACCGGCAGC ATCCAGATGC TTTGAAGTTT
    ACCAGTATTA AAGACACTCC GGAGATGGTC CAGGCCAGAA TTAGTTATAC CCAAGCAGTG
    GATAGGTTGT ACCGAGAGCA AGGAGAAAAC CTAAAGCATC ACTACACGCA GACCACTGAC
    CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT GCCATGAATA TCAGCGAGAC ACGTTATAAG
    GAATCCTGGA GCAAACTTCG AGACGGTGGC TATAAGCTGA GACTGGATGC CATTCCATTC
    CAGGCAGCAA AGGCTTCTGG CGAAATCATA AGTGATTACA AATACAAAGA GGCATTTGAG
    AAAATGAAAG GGCAGATGCT CGGCTCCCGG AGCTTGGAAG ATGATATCAG CCTTGCGCAT
    TCAGTGTACG CGAGCTCTCT GCAGAGCCAA GTGAATTACA AGAAAGACTT TGAACACTCA
    AAGGCGCAGT TCCATCTGCC GTTGGACATG GTAACCCTGG TGCATGCCAA GAAGGCTCAG
    ACCCTGGCCA GCGACCAGGA CTACAGACAC CCACTCCCCC AGTACACGTC CTTGGCTGAG
    GACCTGAGGC TCAGCTGCGC CAAGAGAGCT CACAAGTTGC AGAGTGAGAA TTTGTACCGG
    TCGGACCTGA ACTTTATGCG GGGTGTTGCC TGCGTCATTC CAGGCACGTT AGAGATTGAA
    GGGAGAAAGA GAGCATCTGA ACTCATCAGT GAGAGTAAAT ATCGTCAGCA TCCCCAGTCG
    CTCAAGTACA CAGCCGTGAC AGACACACCC AGCCTCACTC ATGTAAAACT CAGCAACCAG
    ATTACAAACG AGCGCCTCTA TAAAGCAGCG GGGGAAGATG CGAGACACCA GTACACAATG
    ACCCTGGGCC TGCCCGAGTT TGTCCGAGCG AAAACAAACG CGGCCAATCT GAGTGACGCA
    AAATACAAGG AGTCTTGGAG GAATCTCCAT GCTCAAGGCT ACAAGCTAAC AATAGATGCT
    CTGCCCTTCC AGGCGGCTCG AGTCTCTGGA GACATAGCCA GTGATTTTCT CTACAGACAT
    GACTTTGTGA AGGAGCGAGG ACGGCTGATC GGGGCCCAGA GCGTGAGTGA CGACCCCCGG
    CTCCAGCACT GTCAGCGGGT GGGCCAGCTG CAGAGCGAGC TTCAGTACAG GAGGCAGGCG
    GCGGGCAGCA GAGCGCAGTG CCACCTGCCC ATGGACATGG TGCCACTGGT CCACGCCCGG
    AAGGCCCAGG CCCTGGCCAG CGACCTTGAC TACAGGACAC AGTGCCATGC GTTCACAGCG
    CTGCCCGAGG ACCTGAGGAT GGCCTGGGCC AAGAAGGCTC ATGCTCTGCA GAGTGAGCTG
    CGCTACAAGT CGGACCTGAT GGGCATGAAG GGGACAGGAT GGCTGGCGCT GAGTTCCCCA
    CAGATAGAAA GTGCAAAGAA GGCCGGAGAA CTCATCAGTG AGACCAAATA CCGTGAAAAA
    CCAGACACCA TCAAGTTTAC CACAGTGGTT GACTCCCCGG ACCTAGTTCA TGCCAAGAAC
    AGCTACATGC ATTGCAATGA GCGCCTGTAC AGGTCGGGGG ATGCGGAATC CCGACACAGG
    TACACCCTGG TGCCCGACCA CCCCGATTTC ACCAGAGCCC GCCTCAACGC CCTGAATCTG
    AGCGACAAAG TCTACAGACA CTCCTGGGAG CAGACCCGGG CTGGCGGCTA CGACTTCAGG
    CTGGATGCCA TCCCATTCCA GACGGCACGG GCATCGAGGG AAATCGCCAG TGATTTCCGG
    TACAAAGAGG CCTTCCTACG GGACCGGGGC CTGCAGATAG GGTACCGCAG CATCAACGAC
    GACCCGAGGA CGAAGCATTT CCTCAGCGTC GGCAGACTCC AGAGTGACAA CGAGTACAAG
    AAGGACTTCG CCAAGAGTCG GTCCCAGTTC CACAGCCGCC CAGACCAGCC CGGCTTCCTC
    CAGGCCAAGA GGAGCCAGCA GCTGGCCAGC GACGTGCACT ACAGGCAGCC CCTGCCCCAG
    CCCACCTGCG ACCCGGAGCA GCTGGGCCTG AAACACGCGC AGAAGGCCCA CCGGCTGCAG
    AGCGATGTCA AGTACAAATC AGACTTGAAC CTGACCAGAG GTATTGGCTG GACACCTCCT
    GGCTCCTACA AAGTGGAAAT GGCTCGGCGG GCTGCAGAAT TGGCCAACGC GAGGGGCCTG
    GGTCTCCAAG GGGCTTACGG GGGGCCGGAG GCTGTGGAGC CCAGAGATGA TCAGAGCGGG
    TTTGTAAACC CAGATGCCAC CGAGATTCTG CACGTCAAAA GGAGGAAGGC CCCACTGTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841435.1
    CDS179..5161
    Translation

    Target ORF information:

    RefSeq Version XM_024985667.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG AATAAATATA AAGAAAACTA CCAGAACCGC
    ATGAGAGGCC GCTATGAAGG AGTCGGGATG GACAAACGGA TGCTCCATGC TCTGAAAGTT
    GGAAGCCTGG CAAGCAATAT CGCCTACAAG GCTGATTACA AACACGATGT TGTCGACTAC
    AACTACCCGG CCACTCTTAC CCCTTCCTAT CAAACAACAG TGAAACTGGC TCCCTTGAAA
    GACGTCAACT ACAGGCAAAG CATCGACAAG CTGAAGTACA GCTCTGTGAC CAACACGCCT
    CAGATTGTTC AAGCCAAAAT CAATGCCCAG CAACTGAGTC ATGTGAATTA CCGTGCTGAC
    TATGAGAAGA ACAAGTTGAA TTATACGTTG CCCCAGGATG TCCCTCAGCT GGTGAAGGCC
    AGAACCAACG CGGAATTGTT TAGTGAGGTT AAGTACAGAG AAGGCTGGGA GAAGACAAAG
    GGGAAAGGAT TTGAGATGAA GCTGGATGCC ATGTCTCTGC TGGCTGCCAA AGCTTCCGGA
    GAGCTTGCAA GCAATATTAA ATACAAAGAG GAGTATGAAA AAGCAAAAGG CAAAGTCCTA
    GGAACCACAG ACTCGAGGCT GCTGCACTCC CTTCAGGTTG CCAAGATGAG CAGTGAGGTT
    GAATACAAGA AAGGCTTTGA AAAGAGTAAG ACCCACTTTC ACCTGCCCAT GGACATGGTC
    AACATCAGGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAGA
    CTGCACGAAT ACACTGTGCT GCCCGAAGAT ATGAAGACTC TCTGGGCCAA GAAAGCCTAC
    GGGCTCCAGA GCGAGCTGCA GTACAAGGCT GACCTGGCGT GGATGAAAGG GGTCAGGTGG
    CTGACTGAGG GCAGTCTCAA CTTGGAACAG GCCAAGAAGG CTGGACAGCT GGTCAGCGAG
    AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGGCCGA CAGCTCCCAG
    ATGGAGCATG CCAAGAAGAG CCAGGAACTG CAGAGCGGGG TGGCCTACAA GGCAGAGCAC
    GAGCAGTCCG TCCATCAGTA CAGCATTAGC AAAGACGAGC CTCTCTTCCT ACAGGCCCGA
    GCCAACGCTG CCAATCTCAG CGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCG
    AAAGCGTTTG ATCTGCGTCT TGACTCCTTG GCCTTCCTGG CAGCCAAAGC CAAGAGGGAC
    CTGGCCAGTG AGGTGAAGTA CAAAGAAGAT TATGAAAAAT CCAGAGGGAA GCTCATTGGA
    GCTCAGGGTG CCCAGGGGGA TTCGCAAATG AGCCACTCGC TGCAAATGTC CAAGCTCCAG
    AGTGAACTGG AATACAAAAA GGGCTTCGAG GACACCAAGT CTCAGTGCCA CGTCCCCCTG
    GACATGATCC ACCTCGTGCA TGCCCGCAAG GCTCAGCACT TAGCCACAGA CATAGGCTAC
    AAGACAGCAT CCCATCACTT TACAGCTTTG CCCACAGACA TGAAGGTGGA ATGGGCCAAG
    AAGGCTTACG GTTTACAGAG TGATAAGAAG TACCGGCAGC ATCCAGATGC TTTGAAGTTT
    ACCAGTATTA AAGACACTCC GGAGATGGTC CAGGCCAGAA TTAGTTATAC CCAAGCAGTG
    GATAGGTTGT ACCGAGAGCA AGGAGAAAAC CTAAAGCATC ACTACACGCA GACCACTGAC
    CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT GCCATGAATA TCAGCGAGAC ACGTTATAAG
    GAATCCTGGA GCAAACTTCG AGACGGTGGC TATAAGCTGA GACTGGATGC CATTCCATTC
    CAGGCAGCAA AGGCTTCTGG CGAAATCATA AGTGATTACA AATACAAAGA GGCATTTGAG
    AAAATGAAAG GGCAGATGCT CGGCTCCCGG AGCTTGGAAG ATGATATCAG CCTTGCGCAT
    TCAGTGTACG CGAGCTCTCT GCAGAGCCAA GTGAATTACA AGAAAGACTT TGAACACTCA
    AAGGCGCAGT TCCATCTGCC GTTGGACATG GTAACCCTGG TGCATGCCAA GAAGGCTCAG
    ACCCTGGCCA GCGACCAGGA CTACAGACAC CCACTCCCCC AGTACACGTC CTTGGCTGAG
    GACCTGAGGC TCAGCTGCGC CAAGAGAGCT CACAAGTTGC AGAGTGAGAA TTTGTACCGG
    TCGGACCTGA ACTTTATGCG GGGTGTTGCC TGCGTCATTC CAGGCACGTT AGAGATTGAA
    GGGAGAAAGA GAGCATCTGA ACTCATCAGT GAGAGTAAAT ATCGTCAGCA TCCCCAGTCG
    CTCAAGTACA CAGCCGTGAC AGACACACCC AGCCTCACTC ATGTAAAACT CAGCAACCAG
    ATTACAAACG AGCGCCTCTA TAAAGCAGCG GGGGAAGATG CGAGACACCA GTACACAATG
    ACCCTGGGCC TGCCCGAGTT TGTCCGAGCG AAAACAAACG CGGCCAATCT GAGTGACGCA
    AAATACAAGG AGTCTTGGAG GAATCTCCAT GCTCAAGGCT ACAAGCTAAC AATAGATGCT
    CTGCCCTTCC AGGCGGCTCG AGTCTCTGGA GACATAGCCA GTGATTTTCT CTACAGACAT
    GACTTTGTGA AGGAGCGAGG ACGGCTGATC GGGGCCCAGA GCGTGAGTGA CGACCCCCGG
    CTCCAGCACT GTCAGCGGGT GGGCCAGCTG CAGAGCGAGC TTCAGTACAG GAGGCAGGCG
    GCGGGCAGCA GAGCGCAGTG CCACCTGCCC ATGGACATGG TGCCACTGGT CCACGCCCGG
    AAGGCCCAGG CCCTGGCCAG CGACCTTGAC TACAGGACAC AGTGCCATGC GTTCACAGCG
    CTGCCCGAGG ACCTGAGGAT GGCCTGGGCC AAGAAGGCTC ATGCTCTGCA GAGTGAGCTG
    CGCTACAAGT CGGACCTGAT GGGCATGAAG GGGACAGGAT GGCTGGCGCT GAGTTCCCCA
    CAGATAGAAA GTGCAAAGAA GGCCGGAGAA CTCATCAGTG AGACCAAATA CCGTGAAAAA
    CCAGACACCA TCAAGTTTAC CACAGTGGTT GACTCCCCGG ACCTAGTTCA TGCCAAGAAC
    AGCTACATGC ATTGCAATGA GCGCCTGTAC AGGTCGGGGG ATGCGGAATC CCGACACAGG
    TACACCCTGG TGCCCGACCA CCCCGATTTC ACCAGAGCCC GCCTCAACGC CCTGAATCTG
    AGCGACAAAG TCTACAGACA CTCCTGGGAG CAGACCCGGG CTGGCGGCTA CGACTTCAGG
    CTGGATGCCA TCCCATTCCA GACGGCACGG GCATCGAGGG AAATCGCCAG TGATTTCCGG
    TACAAAGAGG CCTTCCTACG GGACCGGGGC CTGCAGATAG GGTACCGCAG CATCAACGAC
    GACCCGAGGA CGAAGCATTT CCTCAGCGTC GGCAGACTCC AGAGTGACAA CGAGTACAAG
    AAGGACTTCG CCAAGAGTCG GTCCCAGTTC CACAGCCGCC CAGACCAGCC CGGCTTCCTC
    CAGGCCAAGA GGAGCCAGCA GCTGGCCAGC GACGTGCACT ACAGGCAGCC CCTGCCCCAG
    CCCACCTGCG ACCCGGAGCA GCTGGGCCTG AAACACGCGC AGAAGGCCCA CCGGCTGCAG
    AGCGATGTCA AGTACAAATC AGACTTGAAC CTGACCAGAG GTATTGGCTG GACACCTCCT
    GGCTCCTACA AAGTGGAAAT GGCTCGGCGG GCTGCAGAAT TGGCCAACGC GAGGGGCCTG
    GGTCTCCAAG GGGCTTACGG GGGGCCGGAG GCTGTGGAGC CCAGAGATGA TCAGAGCGGG
    TTTGTAAACC CAGATGCCAC CGAGATTCTG CACGTCAAAA GGAGGAAGGC CCCACTGTTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40533
    Clone ID Related Accession (Same CDS sequence) NM_001098072.1
    Accession Version NM_001098072.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140607.1. On or before May 23, 2007 this sequence version replaced XM_866509.2, XM_001249416.1. ##Evidence-Data-START## Transcript exon combination :: BC140607.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG GTGGAGTATA AGAAGGATCT GGAAAGTAGT
    AAAGGTCATA GTATCAACTA CTGTGAAACG CCTCAATTCA GGAATGTGTG CAAGATTTCA
    AAATTCACCA GCGATAATAA ATATAAAGAA AACTACCAGA ACCGCATGAG AGGCCGCTAT
    GAAGGAGTCG GGATGGACAA ACGGATGCTC CATGCTCTGA AAGTTGGAAG CCTGGCAAGC
    AATATCGCCT ACAAGGCTGA TTACAAACAC GATGTTGTCG ACTACAACTA CCCGGCCACT
    CTTACCCCTT CCTATCAAAC AACAGTGAAA CTGGCTCCCT TGAAAGACGT CAACTACAGG
    CAAAGCATCG ACAAGCTGAA GTACAGCTCT GTGACCAACA CGCCTCAGAT TGTTCAAGCC
    AAAATCAATG CCCAGCAACT GAGTCATGTG AATTACCGTG CTGACTATGA GAAGAACAAG
    TTGAATTATA CGTTGCCCCA GGATGTCCCT CAGCTGGTGA AGGCCAGAAC CAACGCGGAA
    TTGTTTAGTG AGGTTAAGTA CAGAGAAGGC TGGGAGAAGA CAAAGGGGAA AGGATTTGAG
    ATGAAGCTGG ATGCCATGTC TCTGCTGGCT GCCAAAGCTT CCGGAGAGCT TGCAAGCAAT
    ATTAAATACA AAGAGGAGTA TGAAAAAGCA AAAGGCAAAG TCCTAGGAAC CACAGACTCG
    AGGCTGCTGC ACTCCCTTCA GGTTGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC
    TTTGAAAAGA GTAAGACCCA CTTTCACCTG CCCATGGACA TGGTCAACAT CAGGCATGCT
    AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAGACTGCA CGAATACACT
    GTGCTGCCCG AAGATATGAA GACTCTCTGG GCCAAGAAAG CCTACGGGCT CCAGAGCGAG
    CTGCAGTACA AGGCTGACCT GGCGTGGATG AAAGGGGTCG GGTGGCTGAC TGAGGGCAGT
    CTCAACTTGG AACAGGCCAA GAAGGCTGGA CAGCTGGTCA GCGAGAAAAA CTACAGGCAG
    AGGGTGGATG AGCTGAAGTT CACCAGTGTG GCCGACAGCT CCCAGATGGA GCATGCCAAG
    AAGAGCCAGG AACTGCAGAG CGGGGTGGCC TACAAGGCAG AGCACGAGCA GTCCGTCCAT
    CAGTACAGCA TTAGCAAAGA CGAGCCTCTC TTCCTACAGG CCCGAGCCAA CGCTGCCAAT
    CTCAGCGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCGAAAGC ATTTGATCTG
    CATCTTGACT CCTTGGCCTT CCTGGCAGCC AAAGCCAAGA GGGACCTGGC CAGTGAGGTG
    AAGTACAAAG AAGATTATGA AAAATCCAGA GGGAAGCTCA TTGGAGCTCA GGGTGCCCAG
    GGGGATTCGC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TCCAGAGTGA ACTGGAATAC
    AAGAAGGGCT TCGAGGACAC CAAGTCTCAG TGCCATGTCC CCCTGGACAT GATCCACCTC
    GTGCATGCCC GCAAGGCTCA GCACTTAGCC ACAGACATAG GCTACAAGAC GGCATCCCAT
    CACTTTACAG CTTTGCCCAC AGACATGAAG GTGGAATGGG CCAAGAAGGC TTACGGTTTA
    CAGAGTGATA ACCAATACAG GGCAGATGTG AAGTGGATGA AAGGGACGGG CTGGGTCGCC
    ACCGGGTCAT TAAATGTGGA GCAGGCGAAG AAGGCAGGAG AACTCATTAG CGAGAAGAAG
    TACCGGCAGC ATCCAGATGC TTTGAAGTTT ACCAGTATTA AAGACACTCC GGAGATGGTC
    CAGGCCAGAA TTAGTTATAC CCAAGCAGTG GATAGGTTGT ACCGAGAGCA AGGAGAAAAC
    CTAAAGCATC ACTACACGCA GACCACTGAC CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT
    GCCATGAATA TCAGCGAGAC ACGTTATAAG GAATCCTGGA GCAAACTTCG AGACGGTGGC
    TATAAGCTGA GACTGGATGC CATTCCATTC CAGGCAGCAA AGGCTTCTGG CGAAATCATA
    AGTGATTACA AATACAAAGA GGCATTTGAG AAAATGAAAG GGCAGATGCT CGGCTCCCGG
    AGCTTGGAAG ATGATATCAG CCTTGCGCAT TCAGTGTACG CGAGCTCTCT GCAGAGCCAA
    GTGAATTACA AGAAAGACTT TGAACACTCA AAGGCGCAGT TCCATCTGCC GTTGGACATG
    GTAACCCTGG TGCATGCCAA GAAGGCTCAG ACCCTGGCCA GCGACCAGGA CTACAGACAC
    CCACTCCCCC AGTACACGTC CTTGGCTGAG GACCTGAGGC TCAGCTGCGC CAAGAGAGCT
    CACAAGTTGC AGAGTGAGAA TTTGTACCGG TCGGACCTGA ACTTTATGCG GGGTGTTGCC
    TGCGTCATTC CAGGCACGTT AGAGATTGAA GGGAGAAAGA GAGCATCTGA ACTCATCAGT
    GAGAGTAAAT ATCGTCAGCA TCCCCAGTCG CTCAAGTACA CAGCCGTGAC AGACACACCC
    AGCCTCACTC ATGTAAAACT CAGCAACCAG ATTACAAACG AGCGCCTCTA TAAAGCAGCA
    GGGGAAGATG CGAGACACCA GTACACAATG ACCCTGGGCC TGCCCGAGTT TGTCCGAGCG
    AAAACAAACG CGGCCAATCT GAGTGACGCA AAATACAAGG AGTCTTGGAG GAATCTCCAT
    GCTCAAGGCT ACAAGCTAAC AATAGATGCT CTGCCCTTCC AGGCGGCTCG AGTCTCTGGA
    GACATAGCCA GTGATTTTCT CTACAGACAT GACTTTGTGA AGGAGCGAGG ACGGCTGATC
    GGGGCCCAGA GCGTGAGTGA CGACCCCCGG CTCCAGCACT GTCAGCGGGT GGGCCAGCTG
    CAGAGCGAGC TTCAGTACAG GAGGCAGGCG GCGGGCAGCA GAGCGCAGTG CCACCTGCCC
    ATGGACATGG TGCCACTGGT CCACGCCCGG AAGGCCCAGG CCCTGGCCAG CGACCTTGAC
    TACAGGACAC AGTGCCATGC GTTCACAGCG CTGCCCGAGG ACCTGAGGAT GGCCTGGGCC
    AAGAAGGCTC ATGCTCTGCA GAGTGAGCTG CGCTACAAGT CGGACCTGAT GGGCATGAAG
    GGGACAGGAT GGCTGGCGCT GAGTTCCCCA CAGATAGAAA GTGCAAAGAA GGCCGGAGAA
    CTCATCAGTG AGACCAAATA CCGTGAAAAA CCAGACACCA TCAAGTTTAC CACAGTGGTT
    GACTCCCCGG ACCTAGTTCA TGCCAAGAAC AGCTACATGC ATTGCAATGA GCGCCTGTAC
    AGGTCGGGGG ATGCGGAATC CCGACACAGG TACACCCTGG TGCCCGACCA CCCCGATTTC
    ACCAGAGCCC GCCTCAACGC CCTGAATCTG AGCGACAAAG TCTACAGACA CTCCTGGGAG
    CAGACCCGGG CTGGCGGCTA CGACTTCAGG CTGGATGCCA TCCCATTCCA GACGGCACGG
    GCATCGAGGG AAATCGCCAG TGATTTCCGG TACAAAGAGG CCTTCCTACG GGACCGGGGC
    CTGCAGATAG GGTACCGCAG CATCAACGAC GACCCGAGGA CGAAGCATTT CCTCAGCGTC
    GGCAGACTCC AGAGTGACAA CGAGTACAAG AAGGACTTCG CCAAGAGTCG GTCCCAGTTC
    CACAGCCGCC CAGACCAGCC CGGCTTCCTC CAGGCCAAGA GGAGCCAGCA GCTGGCCAGC
    GACGTGCACT ACAGGCAGCC CCTGCCCCAG CCCACCTGCG ACCCGGAGCA GCTGGGCCTG
    AAACACGCGC AGAAGGCCCA CCGGCTGCAG AGCGATGTCA AGTACAAATC AGACTTGAAC
    CTGACCAGAG GTATTGGCTG GACACCTCCT GGCTCCTACA AAGTGGAAAT GGCTCGGCGG
    GCTGCAGAAT TGGCCAACGC GAGGGGCCTG GGTCTCCAAG GGGCTTACGG GGGGCCGGAG
    GCTGTGGAGC CCAGAGATGA TCAGAGCGGG TTTGTAAACC CAGATGCCAC CGAGATTCTG
    CACGTCAAAA GGAGGAAGGC CCCACTGTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091541.1
    CDS149..5341
    Translation

    Target ORF information:

    RefSeq Version NM_001098072.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098072.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG GTGGAGTATA AGAAGGATCT GGAAAGTAGT
    AAAGGTCATA GTATCAACTA CTGTGAAACG CCTCAATTCA GGAATGTGTG CAAGATTTCA
    AAATTCACCA GCGATAATAA ATATAAAGAA AACTACCAGA ACCGCATGAG AGGCCGCTAT
    GAAGGAGTCG GGATGGACAA ACGGATGCTC CATGCTCTGA AAGTTGGAAG CCTGGCAAGC
    AATATCGCCT ACAAGGCTGA TTACAAACAC GATGTTGTCG ACTACAACTA CCCGGCCACT
    CTTACCCCTT CCTATCAAAC AACAGTGAAA CTGGCTCCCT TGAAAGACGT CAACTACAGG
    CAAAGCATCG ACAAGCTGAA GTACAGCTCT GTGACCAACA CGCCTCAGAT TGTTCAAGCC
    AAAATCAATG CCCAGCAACT GAGTCATGTG AATTACCGTG CTGACTATGA GAAGAACAAG
    TTGAATTATA CGTTGCCCCA GGATGTCCCT CAGCTGGTGA AGGCCAGAAC CAACGCGGAA
    TTGTTTAGTG AGGTTAAGTA CAGAGAAGGC TGGGAGAAGA CAAAGGGGAA AGGATTTGAG
    ATGAAGCTGG ATGCCATGTC TCTGCTGGCT GCCAAAGCTT CCGGAGAGCT TGCAAGCAAT
    ATTAAATACA AAGAGGAGTA TGAAAAAGCA AAAGGCAAAG TCCTAGGAAC CACAGACTCG
    AGGCTGCTGC ACTCCCTTCA GGTTGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC
    TTTGAAAAGA GTAAGACCCA CTTTCACCTG CCCATGGACA TGGTCAACAT CAGGCATGCT
    AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAGACTGCA CGAATACACT
    GTGCTGCCCG AAGATATGAA GACTCTCTGG GCCAAGAAAG CCTACGGGCT CCAGAGCGAG
    CTGCAGTACA AGGCTGACCT GGCGTGGATG AAAGGGGTCG GGTGGCTGAC TGAGGGCAGT
    CTCAACTTGG AACAGGCCAA GAAGGCTGGA CAGCTGGTCA GCGAGAAAAA CTACAGGCAG
    AGGGTGGATG AGCTGAAGTT CACCAGTGTG GCCGACAGCT CCCAGATGGA GCATGCCAAG
    AAGAGCCAGG AACTGCAGAG CGGGGTGGCC TACAAGGCAG AGCACGAGCA GTCCGTCCAT
    CAGTACAGCA TTAGCAAAGA CGAGCCTCTC TTCCTACAGG CCCGAGCCAA CGCTGCCAAT
    CTCAGCGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCGAAAGC ATTTGATCTG
    CATCTTGACT CCTTGGCCTT CCTGGCAGCC AAAGCCAAGA GGGACCTGGC CAGTGAGGTG
    AAGTACAAAG AAGATTATGA AAAATCCAGA GGGAAGCTCA TTGGAGCTCA GGGTGCCCAG
    GGGGATTCGC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TCCAGAGTGA ACTGGAATAC
    AAGAAGGGCT TCGAGGACAC CAAGTCTCAG TGCCATGTCC CCCTGGACAT GATCCACCTC
    GTGCATGCCC GCAAGGCTCA GCACTTAGCC ACAGACATAG GCTACAAGAC GGCATCCCAT
    CACTTTACAG CTTTGCCCAC AGACATGAAG GTGGAATGGG CCAAGAAGGC TTACGGTTTA
    CAGAGTGATA ACCAATACAG GGCAGATGTG AAGTGGATGA AAGGGACGGG CTGGGTCGCC
    ACCGGGTCAT TAAATGTGGA GCAGGCGAAG AAGGCAGGAG AACTCATTAG CGAGAAGAAG
    TACCGGCAGC ATCCAGATGC TTTGAAGTTT ACCAGTATTA AAGACACTCC GGAGATGGTC
    CAGGCCAGAA TTAGTTATAC CCAAGCAGTG GATAGGTTGT ACCGAGAGCA AGGAGAAAAC
    CTAAAGCATC ACTACACGCA GACCACTGAC CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT
    GCCATGAATA TCAGCGAGAC ACGTTATAAG GAATCCTGGA GCAAACTTCG AGACGGTGGC
    TATAAGCTGA GACTGGATGC CATTCCATTC CAGGCAGCAA AGGCTTCTGG CGAAATCATA
    AGTGATTACA AATACAAAGA GGCATTTGAG AAAATGAAAG GGCAGATGCT CGGCTCCCGG
    AGCTTGGAAG ATGATATCAG CCTTGCGCAT TCAGTGTACG CGAGCTCTCT GCAGAGCCAA
    GTGAATTACA AGAAAGACTT TGAACACTCA AAGGCGCAGT TCCATCTGCC GTTGGACATG
    GTAACCCTGG TGCATGCCAA GAAGGCTCAG ACCCTGGCCA GCGACCAGGA CTACAGACAC
    CCACTCCCCC AGTACACGTC CTTGGCTGAG GACCTGAGGC TCAGCTGCGC CAAGAGAGCT
    CACAAGTTGC AGAGTGAGAA TTTGTACCGG TCGGACCTGA ACTTTATGCG GGGTGTTGCC
    TGCGTCATTC CAGGCACGTT AGAGATTGAA GGGAGAAAGA GAGCATCTGA ACTCATCAGT
    GAGAGTAAAT ATCGTCAGCA TCCCCAGTCG CTCAAGTACA CAGCCGTGAC AGACACACCC
    AGCCTCACTC ATGTAAAACT CAGCAACCAG ATTACAAACG AGCGCCTCTA TAAAGCAGCA
    GGGGAAGATG CGAGACACCA GTACACAATG ACCCTGGGCC TGCCCGAGTT TGTCCGAGCG
    AAAACAAACG CGGCCAATCT GAGTGACGCA AAATACAAGG AGTCTTGGAG GAATCTCCAT
    GCTCAAGGCT ACAAGCTAAC AATAGATGCT CTGCCCTTCC AGGCGGCTCG AGTCTCTGGA
    GACATAGCCA GTGATTTTCT CTACAGACAT GACTTTGTGA AGGAGCGAGG ACGGCTGATC
    GGGGCCCAGA GCGTGAGTGA CGACCCCCGG CTCCAGCACT GTCAGCGGGT GGGCCAGCTG
    CAGAGCGAGC TTCAGTACAG GAGGCAGGCG GCGGGCAGCA GAGCGCAGTG CCACCTGCCC
    ATGGACATGG TGCCACTGGT CCACGCCCGG AAGGCCCAGG CCCTGGCCAG CGACCTTGAC
    TACAGGACAC AGTGCCATGC GTTCACAGCG CTGCCCGAGG ACCTGAGGAT GGCCTGGGCC
    AAGAAGGCTC ATGCTCTGCA GAGTGAGCTG CGCTACAAGT CGGACCTGAT GGGCATGAAG
    GGGACAGGAT GGCTGGCGCT GAGTTCCCCA CAGATAGAAA GTGCAAAGAA GGCCGGAGAA
    CTCATCAGTG AGACCAAATA CCGTGAAAAA CCAGACACCA TCAAGTTTAC CACAGTGGTT
    GACTCCCCGG ACCTAGTTCA TGCCAAGAAC AGCTACATGC ATTGCAATGA GCGCCTGTAC
    AGGTCGGGGG ATGCGGAATC CCGACACAGG TACACCCTGG TGCCCGACCA CCCCGATTTC
    ACCAGAGCCC GCCTCAACGC CCTGAATCTG AGCGACAAAG TCTACAGACA CTCCTGGGAG
    CAGACCCGGG CTGGCGGCTA CGACTTCAGG CTGGATGCCA TCCCATTCCA GACGGCACGG
    GCATCGAGGG AAATCGCCAG TGATTTCCGG TACAAAGAGG CCTTCCTACG GGACCGGGGC
    CTGCAGATAG GGTACCGCAG CATCAACGAC GACCCGAGGA CGAAGCATTT CCTCAGCGTC
    GGCAGACTCC AGAGTGACAA CGAGTACAAG AAGGACTTCG CCAAGAGTCG GTCCCAGTTC
    CACAGCCGCC CAGACCAGCC CGGCTTCCTC CAGGCCAAGA GGAGCCAGCA GCTGGCCAGC
    GACGTGCACT ACAGGCAGCC CCTGCCCCAG CCCACCTGCG ACCCGGAGCA GCTGGGCCTG
    AAACACGCGC AGAAGGCCCA CCGGCTGCAG AGCGATGTCA AGTACAAATC AGACTTGAAC
    CTGACCAGAG GTATTGGCTG GACACCTCCT GGCTCCTACA AAGTGGAAAT GGCTCGGCGG
    GCTGCAGAAT TGGCCAACGC GAGGGGCCTG GGTCTCCAAG GGGCTTACGG GGGGCCGGAG
    GCTGTGGAGC CCAGAGATGA TCAGAGCGGG TTTGTAAACC CAGATGCCAC CGAGATTCTG
    CACGTCAAAA GGAGGAAGGC CCCACTGTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33734
    Clone ID Related Accession (Same CDS sequence) XM_005225745.2
    Accession Version XM_005225745.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCATTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAGAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCATG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACGGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAACCAATA CAGGGCAGAT GTGAAGTGGA TGAAAGGGAC GGGCTGGGTC
    GCCACCGGGT CATTAAATGT GGAGCAGGCG AAGAAGGCAG GAGAACTCAT TAGCGAGAAG
    AAGTACCGGC AGCATCCAGA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCGGAGATG
    GTCCAGGCCA GAATTAGTTA TACCCAAGCA GTGGATAGGT TGTACCGAGA GCAAGGAGAA
    AACCTAAAGC ATCACTACAC GCAGACCACT GACCTCCCTG AAGTCCTGCT GGCCAAGCTG
    AATGCCATGA ATATCAGCGA GACACGTTAT AAGGAATCCT GGAGCAAACT TCGAGACGGT
    GGCTATAAGC TGAGACTGGA TGCCATTCCA TTCCAGGCAG CAAAGGCTTC TGGCGAAATC
    ATAAGTGATT ACAAATACAA AGAGGCATTT GAGAAAATGA AAGGGCAGAT GCTCGGCTCC
    CGGAGCTTGG AAGATGATAT CAGCCTTGCG CATTCAGTGT ACGCGAGCTC TCTGCAGAGC
    CAAGTGAATT ACAAGAAAGA CTTTGAACAC TCAAAGGCGC AGTTCCATCT GCCGTTGGAC
    ATGGTAACCC TGGTGCATGC CAAGAAGGCT CAGACCCTGG CCAGCGACCA GGACTACAGA
    CACCCGCTCC CCCAGTACAC GTCCTTGGCT GAGGACCTGA GGCTCAGCTG CGCCAAGAGA
    GCTCACAAGT TGCAGAGTGA GAATTTGTAC CGGTCGGACC TGAACTTTAT GCGGGGTGTT
    GCCTGCGTCA TTCCAGGCAC GTTAGAGATT GAAGGGAGAA AGAGAGCATC TGAACTCATC
    AGTGAGAGTA AATATCGTCA GCATCCCCAG TCGCTCAAGT ACACAGCCGT GACAGACACA
    CCCAGCCTCA CTCATGCAAA ACTCAGCAAC CAGATTACAA ACGAGCGCCT CTATAAAGCA
    GCGGGGGAAG ATGCGAGACA CCAGTACACA ATGACCCTGG GCCTGCCCGA GTTTGTCCGA
    GCGAAAACAA ACGCGGCCAA TCTGAGTGAC GCAAAATACA AGGAGTCTTG GAGGAATCTC
    CATGCTCAAG GCTACAAGCT AACAATAGAT GCTCTGCCCT TCCAGGCGGC TCGAGTCTCT
    GGAGACATAG CCAGTGATTT TCTCTACAGA CATGACTTTG TGAAGGAGCG AGGACGGCTG
    ATCGGGGCCC AGAGCGTGAG TGACGACCCC CGGCTCCAGC ACTGTCAGCG GGTGGGCCAG
    CTGCAGAGCG AGCTTCAGTA CAGGAGGCAG GCGGCAGGCA GCAGAGCGCA GTGCCACCTG
    CCCATGGACA TGGTGCCACT GGTCCATGCC CGGAAGGCCC AGGCCCTGGC CAGCGACCTT
    GACTACAGGA CACAGTGCCA CGCGTTCACA GCGCTGCCCG AGGACCTGAG GATGGCCTGG
    GCCAAGAAGG CCCATGCCCT GCAGAGTGAG CTGCGCTACA AGTCGGACCT GATGGGCATG
    AAGGGGACAG GATGGCTGGC GCTGAGTTCC CCACAGATAG AAAGTGCAAA GAAGGCCGGA
    GAACTCATCA GTGAGACCAA ATACCGTGAA AAACCAGACA CCATCAAGTT TACCACAGTG
    GTTGACTCCC CGGACCTAGT TCATGCCAAG AACAGCTACA TGCATTGCAA TGAGCGCCTG
    TACAGGTCGG GGGATGCGGA ATCCCGACAC AGGTACACCC TGGTGCCCGA CCACCCCGAT
    TTCACCAGAG CCCGCCTCAA CGCCCTGAAT CTGAGCGACA AAGTCTACAG ACACTCCTGG
    GAGCAGACCC GGGCTGGCGG CTACGACTTC AGGCTGGATG CCATCCCATT CCAGACGGCA
    CGGGCATCGA GGGAAATCGC CAGTGATTTC CGGTACAAAG AGGCCTTCCT ACGGGACCGG
    GGCCTGCAGA TAGGGTACCG CAGCATCAAC GACGACCCGA GGACGAAGCA TTTCCTCAGC
    GTCGGCAGAC TCCAGAGTGA CAACGAGTAC AAGAAGGACT TCGCCAAGAG TCGGTCCCAG
    TTCCACAGCC GCCCAGACCA GCCCGGCTTC CTCCAGGCCA AGAGGAGCCA GCAGCTGGCC
    AGCGACGTGC ACTACAGGCA GCCCCTGCCC CAGCCCACCT GCGACCCGGA GCAGCTGGGC
    CTGAAACACG CGCAGAAGGC CCACCGGCTG CAGAGCGATG TCAAGTACAA ATCAGACTTG
    AACCTGACCA GAGGTATTGG CTGGACACCT CCTGGCTCCT ACAAAGTGGA AATGGCTCGG
    CGGGCTGCAG AATTGGCCAA CGCGAGGGGC CTGGGTCTCC AAGGGGCTTA CGGGGGGCCG
    GAGGCTGTGG AGCCCAGAGA TGATCAGAGC GGGTTTGTAA ACCCAGATGC CACCGAGATT
    CTGCACGTCA AAAGGAGGAA GGCCCCACTG TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225802.1
    CDS181..5376
    Translation

    Target ORF information:

    RefSeq Version XM_005225745.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225745.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGGTGGAGT ATAAGAAGGA TCTGGAAAGT
    AGTAAAGGTC ATAGTATCAA CTACTGTGAA ACGCCTCAAT TCAGGAATGT GTGCAAGATT
    TCAAAATTCA CCAGCGATAA TAAATATAAA GAAAACTACC AGAACCGCAT GAGAGGCCGC
    TATGAAGGAG TCGGGATGGA CAAACGGATG CTCCATGCTC TGAAAGTTGG AAGCCTGGCA
    AGCAATATCG CCTACAAGGC TGATTACAAA CACGATGTTG TCGACTACAA CTACCCGGCC
    ACTCTTACCC CTTCCTATCA AACAACAGTG AAACTGGCTC CCTTGAAAGA CGTCAACTAC
    AGGCAAAGCA TCGACAAGCT GAAGTACAGC TCTGTGACCA ACACGCCTCA GATTGTTCAA
    GCCAAAATCA ATGCCCAGCA ACTGAGTCAT GTGAATTACC GTGCTGACTA TGAGAAGAAC
    AAGTTGAATT ATACGTTGCC CCAGGATGTC CCTCAGCTGG TGAAGGCCAG AACCAACGCG
    GAATTGTTTA GTGAGGTTAA GTACAGAGAA GGCTGGGAGA AGACAAAGGG GAAAGGATTT
    GAGATGAAGC TGGATGCCAT GTCTCTGCTG GCTGCCAAAG CTTCCGGAGA GCTTGCAAGC
    AATATTAAAT ACAAAGAGGA GTATGAAAAA GCAAAAGGCA AAGTCCTAGG AACCACAGAC
    TCGAGGCTGC TGCACTCCCT TCAGGTTGCC AAGATGAGCA GTGAGGTTGA ATACAAGAAA
    GGCTTTGAAA AGAGTAAGAC CCACTTTCAC CTGCCCATGG ACATGGTCAA CATCAGGCAT
    GCTAAGAAGG CCCAAGCTCT GGCCAGTGAC CTGGACTACA GGAAAAGACT GCACGAATAC
    ACTGTGCTGC CCGAAGATAT GAAGACTCTC TGGGCCAAGA AAGCCTACGG GCTCCAGAGC
    GAGCTGCAGT ACAAGGCTGA CCTGGCGTGG ATGAAAGGGG TCAGGTGGCT GACTGAGGGC
    AGTCTCAACT TGGAACAGGC CAAGAAGGCT GGACAGCTGG TCAGCGAGAA AAACTACAGG
    CAGAGGGTGG ATGAGCTGAA GTTCACCAGT GTGGCCGACA GCTCCCAGAT GGAGCATGCC
    AAGAAGAGCC AGGAACTGCA GAGCGGGGTG GCCTACAAGG CAGAGCACGA GCAGTCCGTC
    CATCAGTACA GCATTAGCAA AGACGAGCCT CTCTTCCTAC AGGCCCGAGC CAACGCTGCC
    AATCTCAGCG AGAAACTGTA TAAGAGCAGC TGGGAAAACC AGAAGGCGAA AGCATTTGAT
    CTGCGTCTTG ACTCCTTGGC CTTCCTGGCA GCCAAAGCCA AGAGGGACCT GGCCAGTGAG
    GTGAAGTACA AAGAAGATTA TGAAAAATCC AGAGGGAAGC TCATTGGAGC TCAGGGTGCC
    CAGGGGGATT CGCAAATGAG CCACTCGCTG CAAATGTCCA AGCTCCAGAG TGAACTGGAA
    TACAAGAAGG GCTTCGAGGA CACCAAGTCT CAGTGCCATG TCCCCCTGGA CATGATCCAC
    CTCGTGCATG CCCGCAAGGC TCAGCACTTA GCCACAGACA TAGGCTACAA GACGGCATCC
    CATCACTTTA CAGCTTTGCC CACAGACATG AAGGTGGAAT GGGCCAAGAA GGCTTACGGT
    TTACAGAGTG ATAACCAATA CAGGGCAGAT GTGAAGTGGA TGAAAGGGAC GGGCTGGGTC
    GCCACCGGGT CATTAAATGT GGAGCAGGCG AAGAAGGCAG GAGAACTCAT TAGCGAGAAG
    AAGTACCGGC AGCATCCAGA TGCTTTGAAG TTTACCAGTA TTAAAGACAC TCCGGAGATG
    GTCCAGGCCA GAATTAGTTA TACCCAAGCA GTGGATAGGT TGTACCGAGA GCAAGGAGAA
    AACCTAAAGC ATCACTACAC GCAGACCACT GACCTCCCTG AAGTCCTGCT GGCCAAGCTG
    AATGCCATGA ATATCAGCGA GACACGTTAT AAGGAATCCT GGAGCAAACT TCGAGACGGT
    GGCTATAAGC TGAGACTGGA TGCCATTCCA TTCCAGGCAG CAAAGGCTTC TGGCGAAATC
    ATAAGTGATT ACAAATACAA AGAGGCATTT GAGAAAATGA AAGGGCAGAT GCTCGGCTCC
    CGGAGCTTGG AAGATGATAT CAGCCTTGCG CATTCAGTGT ACGCGAGCTC TCTGCAGAGC
    CAAGTGAATT ACAAGAAAGA CTTTGAACAC TCAAAGGCGC AGTTCCATCT GCCGTTGGAC
    ATGGTAACCC TGGTGCATGC CAAGAAGGCT CAGACCCTGG CCAGCGACCA GGACTACAGA
    CACCCGCTCC CCCAGTACAC GTCCTTGGCT GAGGACCTGA GGCTCAGCTG CGCCAAGAGA
    GCTCACAAGT TGCAGAGTGA GAATTTGTAC CGGTCGGACC TGAACTTTAT GCGGGGTGTT
    GCCTGCGTCA TTCCAGGCAC GTTAGAGATT GAAGGGAGAA AGAGAGCATC TGAACTCATC
    AGTGAGAGTA AATATCGTCA GCATCCCCAG TCGCTCAAGT ACACAGCCGT GACAGACACA
    CCCAGCCTCA CTCATGCAAA ACTCAGCAAC CAGATTACAA ACGAGCGCCT CTATAAAGCA
    GCGGGGGAAG ATGCGAGACA CCAGTACACA ATGACCCTGG GCCTGCCCGA GTTTGTCCGA
    GCGAAAACAA ACGCGGCCAA TCTGAGTGAC GCAAAATACA AGGAGTCTTG GAGGAATCTC
    CATGCTCAAG GCTACAAGCT AACAATAGAT GCTCTGCCCT TCCAGGCGGC TCGAGTCTCT
    GGAGACATAG CCAGTGATTT TCTCTACAGA CATGACTTTG TGAAGGAGCG AGGACGGCTG
    ATCGGGGCCC AGAGCGTGAG TGACGACCCC CGGCTCCAGC ACTGTCAGCG GGTGGGCCAG
    CTGCAGAGCG AGCTTCAGTA CAGGAGGCAG GCGGCAGGCA GCAGAGCGCA GTGCCACCTG
    CCCATGGACA TGGTGCCACT GGTCCATGCC CGGAAGGCCC AGGCCCTGGC CAGCGACCTT
    GACTACAGGA CACAGTGCCA CGCGTTCACA GCGCTGCCCG AGGACCTGAG GATGGCCTGG
    GCCAAGAAGG CCCATGCCCT GCAGAGTGAG CTGCGCTACA AGTCGGACCT GATGGGCATG
    AAGGGGACAG GATGGCTGGC GCTGAGTTCC CCACAGATAG AAAGTGCAAA GAAGGCCGGA
    GAACTCATCA GTGAGACCAA ATACCGTGAA AAACCAGACA CCATCAAGTT TACCACAGTG
    GTTGACTCCC CGGACCTAGT TCATGCCAAG AACAGCTACA TGCATTGCAA TGAGCGCCTG
    TACAGGTCGG GGGATGCGGA ATCCCGACAC AGGTACACCC TGGTGCCCGA CCACCCCGAT
    TTCACCAGAG CCCGCCTCAA CGCCCTGAAT CTGAGCGACA AAGTCTACAG ACACTCCTGG
    GAGCAGACCC GGGCTGGCGG CTACGACTTC AGGCTGGATG CCATCCCATT CCAGACGGCA
    CGGGCATCGA GGGAAATCGC CAGTGATTTC CGGTACAAAG AGGCCTTCCT ACGGGACCGG
    GGCCTGCAGA TAGGGTACCG CAGCATCAAC GACGACCCGA GGACGAAGCA TTTCCTCAGC
    GTCGGCAGAC TCCAGAGTGA CAACGAGTAC AAGAAGGACT TCGCCAAGAG TCGGTCCCAG
    TTCCACAGCC GCCCAGACCA GCCCGGCTTC CTCCAGGCCA AGAGGAGCCA GCAGCTGGCC
    AGCGACGTGC ACTACAGGCA GCCCCTGCCC CAGCCCACCT GCGACCCGGA GCAGCTGGGC
    CTGAAACACG CGCAGAAGGC CCACCGGCTG CAGAGCGATG TCAAGTACAA ATCAGACTTG
    AACCTGACCA GAGGTATTGG CTGGACACCT CCTGGCTCCT ACAAAGTGGA AATGGCTCGG
    CGGGCTGCAG AATTGGCCAA CGCGAGGGGC CTGGGTCTCC AAGGGGCTTA CGGGGGGCCG
    GAGGCTGTGG AGCCCAGAGA TGATCAGAGC GGGTTTGTAA ACCCAGATGC CACCGAGATT
    CTGCACGTCA AAAGGAGGAA GGCCCCACTG TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33735
    Clone ID Related Accession (Same CDS sequence) XM_005225746.2
    Accession Version XM_005225746.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGAATAAAT ATAAAGAAAA CTACCAGAAC
    CGCATGAGAG GCCGCTATGA AGGAGTCGGG ATGGACAAAC GGATGCTCCA TGCTCTGAAA
    GTTGGAAGCC TGGCAAGCAA TATCGCCTAC AAGGCTGATT ACAAACACGA TGTTGTCGAC
    TACAACTACC CGGCCACTCT TACCCCTTCC TATCAAACAA CAGTGAAACT GGCTCCCTTG
    AAAGACGTCA ACTACAGGCA AAGCATCGAC AAGCTGAAGT ACAGCTCTGT GACCAACACG
    CCTCAGATTG TTCAAGCCAA AATCAATGCC CAGCAACTGA GTCATGTGAA TTACCGTGCT
    GACTATGAGA AGAACAAGTT GAATTATACG TTGCCCCAGG ATGTCCCTCA GCTGGTGAAG
    GCCAGAACCA ACGCGGAATT GTTTAGTGAG GTTAAGTACA GAGAAGGCTG GGAGAAGACA
    AAGGGGAAAG GATTTGAGAT GAAGCTGGAT GCCATGTCTC TGCTGGCTGC CAAAGCTTCC
    GGAGAGCTTG CAAGCAATAT TAAATACAAA GAGGAGTATG AAAAAGCAAA AGGCAAAGTC
    CTAGGAACCA CAGACTCGAG GCTGCTGCAC TCCCTTCAGG TTGCCAAGAT GAGCAGTGAG
    GTTGAATACA AGAAAGGCTT TGAAAAGAGT AAGACCCACT TTCACCTGCC CATGGACATG
    GTCAACATCA GGCATGCTAA GAAGGCCCAA GCTCTGGCCA GTGACCTGGA CTACAGGAAA
    AGACTGCACG AATACACTGT GCTGCCCGAA GATATGAAGA CTCTCTGGGC CAAGAAAGCC
    TACGGGCTCC AGAGCGAGCT GCAGTACAAG GCTGACCTGG CGTGGATGAA AGGGGTCAGG
    TGGCTGACTG AGGGCAGTCT CAACTTGGAA CAGGCCAAGA AGGCTGGACA GCTGGTCAGC
    GAGAAAAACT ACAGGCAGAG GGTGGATGAG CTGAAGTTCA CCAGTGTGGC CGACAGCTCC
    CAGATGGAGC ATGCCAAGAA GAGCCAGGAA CTGCAGAGCG GGGTGGCCTA CAAGGCAGAG
    CACGAGCAGT CCGTCCATCA GTACAGCATT AGCAAAGACG AGCCTCTCTT CCTACAGGCC
    CGAGCCAACG CTGCCAATCT CAGCGAGAAA CTGTATAAGA GCAGCTGGGA AAACCAGAAG
    GCGAAAGCAT TTGATCTGCG TCTTGACTCC TTGGCCTTCC TGGCAGCCAA AGCCAAGAGG
    GACCTGGCCA GTGAGGTGAA GTACAAAGAA GATTATGAAA AATCCAGAGG GAAGCTCATT
    GGAGCTCAGG GTGCCCAGGG GGATTCGCAA ATGAGCCACT CGCTGCAAAT GTCCAAGCTC
    CAGAGTGAAC TGGAATACAA GAAGGGCTTC GAGGACACCA AGTCTCAGTG CCATGTCCCC
    CTGGACATGA TCCACCTCGT GCATGCCCGC AAGGCTCAGC ACTTAGCCAC AGACATAGGC
    TACAAGACGG CATCCCATCA CTTTACAGCT TTGCCCACAG ACATGAAGGT GGAATGGGCC
    AAGAAGGCTT ACGGTTTACA GAGTGATAAC CAATACAGGG CAGATGTGAA GTGGATGAAA
    GGGACGGGCT GGGTCGCCAC CGGGTCATTA AATGTGGAGC AGGCGAAGAA GGCAGGAGAA
    CTCATTAGCG AGAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC GCTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GCAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC AGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ATGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCACGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCCCAT GCCCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225803.1
    CDS181..5271
    Translation

    Target ORF information:

    RefSeq Version XM_005225746.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225746.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGCA GGGTTACTCA
    GAAGAGTATG GCGAGCACAG AGGAAAGGGC AGCTTCCCGG CCATGATCAC CCCAGCTTAT
    CAGAACGCCA AGAAAGCCAA CGAACTCGCT AGTGATATAA AATACAGGCA GGACTTCAAT
    AAGATGAAGG GCGCTGCACA TTATCATTCC CTCCCAGCCC AGGACAACTT GGTTCTGAAG
    CGGGCTCAGA GCGTGAACAA GCTCGTGAGT GAGAATAAAT ATAAAGAAAA CTACCAGAAC
    CGCATGAGAG GCCGCTATGA AGGAGTCGGG ATGGACAAAC GGATGCTCCA TGCTCTGAAA
    GTTGGAAGCC TGGCAAGCAA TATCGCCTAC AAGGCTGATT ACAAACACGA TGTTGTCGAC
    TACAACTACC CGGCCACTCT TACCCCTTCC TATCAAACAA CAGTGAAACT GGCTCCCTTG
    AAAGACGTCA ACTACAGGCA AAGCATCGAC AAGCTGAAGT ACAGCTCTGT GACCAACACG
    CCTCAGATTG TTCAAGCCAA AATCAATGCC CAGCAACTGA GTCATGTGAA TTACCGTGCT
    GACTATGAGA AGAACAAGTT GAATTATACG TTGCCCCAGG ATGTCCCTCA GCTGGTGAAG
    GCCAGAACCA ACGCGGAATT GTTTAGTGAG GTTAAGTACA GAGAAGGCTG GGAGAAGACA
    AAGGGGAAAG GATTTGAGAT GAAGCTGGAT GCCATGTCTC TGCTGGCTGC CAAAGCTTCC
    GGAGAGCTTG CAAGCAATAT TAAATACAAA GAGGAGTATG AAAAAGCAAA AGGCAAAGTC
    CTAGGAACCA CAGACTCGAG GCTGCTGCAC TCCCTTCAGG TTGCCAAGAT GAGCAGTGAG
    GTTGAATACA AGAAAGGCTT TGAAAAGAGT AAGACCCACT TTCACCTGCC CATGGACATG
    GTCAACATCA GGCATGCTAA GAAGGCCCAA GCTCTGGCCA GTGACCTGGA CTACAGGAAA
    AGACTGCACG AATACACTGT GCTGCCCGAA GATATGAAGA CTCTCTGGGC CAAGAAAGCC
    TACGGGCTCC AGAGCGAGCT GCAGTACAAG GCTGACCTGG CGTGGATGAA AGGGGTCAGG
    TGGCTGACTG AGGGCAGTCT CAACTTGGAA CAGGCCAAGA AGGCTGGACA GCTGGTCAGC
    GAGAAAAACT ACAGGCAGAG GGTGGATGAG CTGAAGTTCA CCAGTGTGGC CGACAGCTCC
    CAGATGGAGC ATGCCAAGAA GAGCCAGGAA CTGCAGAGCG GGGTGGCCTA CAAGGCAGAG
    CACGAGCAGT CCGTCCATCA GTACAGCATT AGCAAAGACG AGCCTCTCTT CCTACAGGCC
    CGAGCCAACG CTGCCAATCT CAGCGAGAAA CTGTATAAGA GCAGCTGGGA AAACCAGAAG
    GCGAAAGCAT TTGATCTGCG TCTTGACTCC TTGGCCTTCC TGGCAGCCAA AGCCAAGAGG
    GACCTGGCCA GTGAGGTGAA GTACAAAGAA GATTATGAAA AATCCAGAGG GAAGCTCATT
    GGAGCTCAGG GTGCCCAGGG GGATTCGCAA ATGAGCCACT CGCTGCAAAT GTCCAAGCTC
    CAGAGTGAAC TGGAATACAA GAAGGGCTTC GAGGACACCA AGTCTCAGTG CCATGTCCCC
    CTGGACATGA TCCACCTCGT GCATGCCCGC AAGGCTCAGC ACTTAGCCAC AGACATAGGC
    TACAAGACGG CATCCCATCA CTTTACAGCT TTGCCCACAG ACATGAAGGT GGAATGGGCC
    AAGAAGGCTT ACGGTTTACA GAGTGATAAC CAATACAGGG CAGATGTGAA GTGGATGAAA
    GGGACGGGCT GGGTCGCCAC CGGGTCATTA AATGTGGAGC AGGCGAAGAA GGCAGGAGAA
    CTCATTAGCG AGAAGAAGTA CCGGCAGCAT CCAGATGCTT TGAAGTTTAC CAGTATTAAA
    GACACTCCGG AGATGGTCCA GGCCAGAATT AGTTATACCC AAGCAGTGGA TAGGTTGTAC
    CGAGAGCAAG GAGAAAACCT AAAGCATCAC TACACGCAGA CCACTGACCT CCCTGAAGTC
    CTGCTGGCCA AGCTGAATGC CATGAATATC AGCGAGACAC GTTATAAGGA ATCCTGGAGC
    AAACTTCGAG ACGGTGGCTA TAAGCTGAGA CTGGATGCCA TTCCATTCCA GGCAGCAAAG
    GCTTCTGGCG AAATCATAAG TGATTACAAA TACAAAGAGG CATTTGAGAA AATGAAAGGG
    CAGATGCTCG GCTCCCGGAG CTTGGAAGAT GATATCAGCC TTGCGCATTC AGTGTACGCG
    AGCTCTCTGC AGAGCCAAGT GAATTACAAG AAAGACTTTG AACACTCAAA GGCGCAGTTC
    CATCTGCCGT TGGACATGGT AACCCTGGTG CATGCCAAGA AGGCTCAGAC CCTGGCCAGC
    GACCAGGACT ACAGACACCC GCTCCCCCAG TACACGTCCT TGGCTGAGGA CCTGAGGCTC
    AGCTGCGCCA AGAGAGCTCA CAAGTTGCAG AGTGAGAATT TGTACCGGTC GGACCTGAAC
    TTTATGCGGG GTGTTGCCTG CGTCATTCCA GGCACGTTAG AGATTGAAGG GAGAAAGAGA
    GCATCTGAAC TCATCAGTGA GAGTAAATAT CGTCAGCATC CCCAGTCGCT CAAGTACACA
    GCCGTGACAG ACACACCCAG CCTCACTCAT GCAAAACTCA GCAACCAGAT TACAAACGAG
    CGCCTCTATA AAGCAGCGGG GGAAGATGCG AGACACCAGT ACACAATGAC CCTGGGCCTG
    CCCGAGTTTG TCCGAGCGAA AACAAACGCG GCCAATCTGA GTGACGCAAA ATACAAGGAG
    TCTTGGAGGA ATCTCCATGC TCAAGGCTAC AAGCTAACAA TAGATGCTCT GCCCTTCCAG
    GCGGCTCGAG TCTCTGGAGA CATAGCCAGT GATTTTCTCT ACAGACATGA CTTTGTGAAG
    GAGCGAGGAC GGCTGATCGG GGCCCAGAGC GTGAGTGACG ACCCCCGGCT CCAGCACTGT
    CAGCGGGTGG GCCAGCTGCA GAGCGAGCTT CAGTACAGGA GGCAGGCGGC AGGCAGCAGA
    GCGCAGTGCC ACCTGCCCAT GGACATGGTG CCACTGGTCC ATGCCCGGAA GGCCCAGGCC
    CTGGCCAGCG ACCTTGACTA CAGGACACAG TGCCACGCGT TCACAGCGCT GCCCGAGGAC
    CTGAGGATGG CCTGGGCCAA GAAGGCCCAT GCCCTGCAGA GTGAGCTGCG CTACAAGTCG
    GACCTGATGG GCATGAAGGG GACAGGATGG CTGGCGCTGA GTTCCCCACA GATAGAAAGT
    GCAAAGAAGG CCGGAGAACT CATCAGTGAG ACCAAATACC GTGAAAAACC AGACACCATC
    AAGTTTACCA CAGTGGTTGA CTCCCCGGAC CTAGTTCATG CCAAGAACAG CTACATGCAT
    TGCAATGAGC GCCTGTACAG GTCGGGGGAT GCGGAATCCC GACACAGGTA CACCCTGGTG
    CCCGACCACC CCGATTTCAC CAGAGCCCGC CTCAACGCCC TGAATCTGAG CGACAAAGTC
    TACAGACACT CCTGGGAGCA GACCCGGGCT GGCGGCTACG ACTTCAGGCT GGATGCCATC
    CCATTCCAGA CGGCACGGGC ATCGAGGGAA ATCGCCAGTG ATTTCCGGTA CAAAGAGGCC
    TTCCTACGGG ACCGGGGCCT GCAGATAGGG TACCGCAGCA TCAACGACGA CCCGAGGACG
    AAGCATTTCC TCAGCGTCGG CAGACTCCAG AGTGACAACG AGTACAAGAA GGACTTCGCC
    AAGAGTCGGT CCCAGTTCCA CAGCCGCCCA GACCAGCCCG GCTTCCTCCA GGCCAAGAGG
    AGCCAGCAGC TGGCCAGCGA CGTGCACTAC AGGCAGCCCC TGCCCCAGCC CACCTGCGAC
    CCGGAGCAGC TGGGCCTGAA ACACGCGCAG AAGGCCCACC GGCTGCAGAG CGATGTCAAG
    TACAAATCAG ACTTGAACCT GACCAGAGGT ATTGGCTGGA CACCTCCTGG CTCCTACAAA
    GTGGAAATGG CTCGGCGGGC TGCAGAATTG GCCAACGCGA GGGGCCTGGG TCTCCAAGGG
    GCTTACGGGG GGCCGGAGGC TGTGGAGCCC AGAGATGATC AGAGCGGGTT TGTAAACCCA
    GATGCCACCG AGATTCTGCA CGTCAAAAGG AGGAAGGCCC CACTGTTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33736
    Clone ID Related Accession (Same CDS sequence) XM_005225747.2
    Accession Version XM_005225747.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nebulin-related-anchoring protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG AATAAATATA AAGAAAACTA CCAGAACCGC
    ATGAGAGGCC GCTATGAAGG AGTCGGGATG GACAAACGGA TGCTCCATGC TCTGAAAGTT
    GGAAGCCTGG CAAGCAATAT CGCCTACAAG GCTGATTACA AACACGATGT TGTCGACTAC
    AACTACCCGG CCACTCTTAC CCCTTCCTAT CAAACAACAG TGAAACTGGC TCCCTTGAAA
    GACGTCAACT ACAGGCAAAG CATCGACAAG CTGAAGTACA GCTCTGTGAC CAACACGCCT
    CAGATTGTTC AAGCCAAAAT CAATGCCCAG CAACTGAGTC ATGTGAATTA CCGTGCTGAC
    TATGAGAAGA ACAAGTTGAA TTATACGTTG CCCCAGGATG TCCCTCAGCT GGTGAAGGCC
    AGAACCAACG CGGAATTGTT TAGTGAGGTT AAGTACAGAG AAGGCTGGGA GAAGACAAAG
    GGGAAAGGAT TTGAGATGAA GCTGGATGCC ATGTCTCTGC TGGCTGCCAA AGCTTCCGGA
    GAGCTTGCAA GCAATATTAA ATACAAAGAG GAGTATGAAA AAGCAAAAGG CAAAGTCCTA
    GGAACCACAG ACTCGAGGCT GCTGCACTCC CTTCAGGTTG CCAAGATGAG CAGTGAGGTT
    GAATACAAGA AAGGCTTTGA AAAGAGTAAG ACCCACTTTC ACCTGCCCAT GGACATGGTC
    AACATCAGGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAGA
    CTGCACGAAT ACACTGTGCT GCCCGAAGAT ATGAAGACTC TCTGGGCCAA GAAAGCCTAC
    GGGCTCCAGA GCGAGCTGCA GTACAAGGCT GACCTGGCGT GGATGAAAGG GGTCAGGTGG
    CTGACTGAGG GCAGTCTCAA CTTGGAACAG GCCAAGAAGG CTGGACAGCT GGTCAGCGAG
    AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGGCCGA CAGCTCCCAG
    ATGGAGCATG CCAAGAAGAG CCAGGAACTG CAGAGCGGGG TGGCCTACAA GGCAGAGCAC
    GAGCAGTCCG TCCATCAGTA CAGCATTAGC AAAGACGAGC CTCTCTTCCT ACAGGCCCGA
    GCCAACGCTG CCAATCTCAG CGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCG
    AAAGCATTTG ATCTGCGTCT TGACTCCTTG GCCTTCCTGG CAGCCAAAGC CAAGAGGGAC
    CTGGCCAGTG AGGTGAAGTA CAAAGAAGAT TATGAAAAAT CCAGAGGGAA GCTCATTGGA
    GCTCAGGGTG CCCAGGGGGA TTCGCAAATG AGCCACTCGC TGCAAATGTC CAAGCTCCAG
    AGTGAACTGG AATACAAGAA GGGCTTCGAG GACACCAAGT CTCAGTGCCA TGTCCCCCTG
    GACATGATCC ACCTCGTGCA TGCCCGCAAG GCTCAGCACT TAGCCACAGA CATAGGCTAC
    AAGACGGCAT CCCATCACTT TACAGCTTTG CCCACAGACA TGAAGGTGGA ATGGGCCAAG
    AAGGCTTACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGG
    ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC
    ATTAGCGAGA AGAAGTACCG GCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC
    ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTGTACCGA
    GAGCAAGGAG AAAACCTAAA GCATCACTAC ACGCAGACCA CTGACCTCCC TGAAGTCCTG
    CTGGCCAAGC TGAATGCCAT GAATATCAGC GAGACACGTT ATAAGGAATC CTGGAGCAAA
    CTTCGAGACG GTGGCTATAA GCTGAGACTG GATGCCATTC CATTCCAGGC AGCAAAGGCT
    TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG
    ATGCTCGGCT CCCGGAGCTT GGAAGATGAT ATCAGCCTTG CGCATTCAGT GTACGCGAGC
    TCTCTGCAGA GCCAAGTGAA TTACAAGAAA GACTTTGAAC ACTCAAAGGC GCAGTTCCAT
    CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGACCCT GGCCAGCGAC
    CAGGACTACA GACACCCGCT CCCCCAGTAC ACGTCCTTGG CTGAGGACCT GAGGCTCAGC
    TGCGCCAAGA GAGCTCACAA GTTGCAGAGT GAGAATTTGT ACCGGTCGGA CCTGAACTTT
    ATGCGGGGTG TTGCCTGCGT CATTCCAGGC ACGTTAGAGA TTGAAGGGAG AAAGAGAGCA
    TCTGAACTCA TCAGTGAGAG TAAATATCGT CAGCATCCCC AGTCGCTCAA GTACACAGCC
    GTGACAGACA CACCCAGCCT CACTCATGCA AAACTCAGCA ACCAGATTAC AAACGAGCGC
    CTCTATAAAG CAGCGGGGGA AGATGCGAGA CACCAGTACA CAATGACCCT GGGCCTGCCC
    GAGTTTGTCC GAGCGAAAAC AAACGCGGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT
    TGGAGGAATC TCCATGCTCA AGGCTACAAG CTAACAATAG ATGCTCTGCC CTTCCAGGCG
    GCTCGAGTCT CTGGAGACAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG
    CGAGGACGGC TGATCGGGGC CCAGAGCGTG AGTGACGACC CCCGGCTCCA GCACTGTCAG
    CGGGTGGGCC AGCTGCAGAG CGAGCTTCAG TACAGGAGGC AGGCGGCAGG CAGCAGAGCG
    CAGTGCCACC TGCCCATGGA CATGGTGCCA CTGGTCCATG CCCGGAAGGC CCAGGCCCTG
    GCCAGCGACC TTGACTACAG GACACAGTGC CACGCGTTCA CAGCGCTGCC CGAGGACCTG
    AGGATGGCCT GGGCCAAGAA GGCCCATGCC CTGCAGAGTG AGCTGCGCTA CAAGTCGGAC
    CTGATGGGCA TGAAGGGGAC AGGATGGCTG GCGCTGAGTT CCCCACAGAT AGAAAGTGCA
    AAGAAGGCCG GAGAACTCAT CAGTGAGACC AAATACCGTG AAAAACCAGA CACCATCAAG
    TTTACCACAG TGGTTGACTC CCCGGACCTA GTTCATGCCA AGAACAGCTA CATGCATTGC
    AATGAGCGCC TGTACAGGTC GGGGGATGCG GAATCCCGAC ACAGGTACAC CCTGGTGCCC
    GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCCCTGA ATCTGAGCGA CAAAGTCTAC
    AGACACTCCT GGGAGCAGAC CCGGGCTGGC GGCTACGACT TCAGGCTGGA TGCCATCCCA
    TTCCAGACGG CACGGGCATC GAGGGAAATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC
    CTACGGGACC GGGGCCTGCA GATAGGGTAC CGCAGCATCA ACGACGACCC GAGGACGAAG
    CATTTCCTCA GCGTCGGCAG ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTCGCCAAG
    AGTCGGTCCC AGTTCCACAG CCGCCCAGAC CAGCCCGGCT TCCTCCAGGC CAAGAGGAGC
    CAGCAGCTGG CCAGCGACGT GCACTACAGG CAGCCCCTGC CCCAGCCCAC CTGCGACCCG
    GAGCAGCTGG GCCTGAAACA CGCGCAGAAG GCCCACCGGC TGCAGAGCGA TGTCAAGTAC
    AAATCAGACT TGAACCTGAC CAGAGGTATT GGCTGGACAC CTCCTGGCTC CTACAAAGTG
    GAAATGGCTC GGCGGGCTGC AGAATTGGCC AACGCGAGGG GCCTGGGTCT CCAAGGGGCT
    TACGGGGGGC CGGAGGCTGT GGAGCCCAGA GATGATCAGA GCGGGTTTGT AAACCCAGAT
    GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCCCCAC TGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225804.1
    CDS181..5268
    Translation

    Target ORF information:

    RefSeq Version XM_005225747.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nebulin related anchoring protein (NRAP), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225747.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAATGTGC AGGCTTGTTC TAGGTGTGGG TACGGGGTGT ACCCTGCTGA GAAGATCAAC 
    TGTCTAGACC AGATATGGCA CAAAGCTTGT TTTCACTGTG AAGTTTGCAA GATGATGCTA
    TCAGTGAATA ACTTTGTGAG TTACCAGAAA AAGCCATACT GTCACGCCCA TAACCCCAAG
    AACAACACTT TTACAAGTGT CTATTACACT CCATTAAATC TAAATGTGCG GAAACCTCCA
    GAGGCCATTT GTGGGATCGG TGGCCAGGAG GATGGTGAAC GGTTTAAGTC GGTTTTTCAC
    TGGGACATGA AATCCAAGGA TGAGGCCGCC GCACCTAACA GGCAACCACA GGTGGATGAG
    AGAGCCTATT GGAGTGGATA CAGGGAAGGG GACGCCTGGT GCCCAGGAGC TCTTCCAGAC
    CCTGAAATCG TAAGGATGGT GGAGGCACGA AAGTCACTGG GTGAGGAGTA TCCAGAAGAC
    TATGAGCAAC AGAGAGGAAA AGGGAGTTTT CCAGCCATGA TCACACCTGC TTATCAAAGG
    GCCAAGATCG CCAACCAGCT GGCCAGCCAA GTGGAGTACA AGAGAGGGCA TGATGAGCGC
    ATCTCCAGGT TCAGCACGGT GGCGGATACC CCCGAGCTGC TCCGGGCCAA GGCTGGTGGA
    CAGCTTCAAA GTGATGTGAG ATACACAGAG GCCTATGAAC AGCAGAGAGG GAAAGGCAGT
    TTTCCTGCTA TGATCACACC TGCTTATCAG ATAGCCAAAA GAGCCAACGA GCTGGCAAGT
    GATGTGAGAT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTGG CCCTGCTGCT
    GGAGCTGAGG GTCCGTTGCC AAAGGAATAC ATGGACCAGT ATGGCCAGGG TTACTCAGAA
    GAGTATGGCG AGCACAGAGG AAAGGGCAGC TTCCCGGCCA TGATCACCCC AGCTTATCAG
    AACGCCAAGA AAGCCAACGA ACTCGCTAGT GATATAAAAT ACAGGCAGGA CTTCAATAAG
    ATGAAGGGCG CTGCACATTA TCATTCCCTC CCAGCCCAGG ACAACTTGGT TCTGAAGCGG
    GCTCAGAGCG TGAACAAGCT CGTGAGTGAG AATAAATATA AAGAAAACTA CCAGAACCGC
    ATGAGAGGCC GCTATGAAGG AGTCGGGATG GACAAACGGA TGCTCCATGC TCTGAAAGTT
    GGAAGCCTGG CAAGCAATAT CGCCTACAAG GCTGATTACA AACACGATGT TGTCGACTAC
    AACTACCCGG CCACTCTTAC CCCTTCCTAT CAAACAACAG TGAAACTGGC TCCCTTGAAA
    GACGTCAACT ACAGGCAAAG CATCGACAAG CTGAAGTACA GCTCTGTGAC CAACACGCCT
    CAGATTGTTC AAGCCAAAAT CAATGCCCAG CAACTGAGTC ATGTGAATTA CCGTGCTGAC
    TATGAGAAGA ACAAGTTGAA TTATACGTTG CCCCAGGATG TCCCTCAGCT GGTGAAGGCC
    AGAACCAACG CGGAATTGTT TAGTGAGGTT AAGTACAGAG AAGGCTGGGA GAAGACAAAG
    GGGAAAGGAT TTGAGATGAA GCTGGATGCC ATGTCTCTGC TGGCTGCCAA AGCTTCCGGA
    GAGCTTGCAA GCAATATTAA ATACAAAGAG GAGTATGAAA AAGCAAAAGG CAAAGTCCTA
    GGAACCACAG ACTCGAGGCT GCTGCACTCC CTTCAGGTTG CCAAGATGAG CAGTGAGGTT
    GAATACAAGA AAGGCTTTGA AAAGAGTAAG ACCCACTTTC ACCTGCCCAT GGACATGGTC
    AACATCAGGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAGA
    CTGCACGAAT ACACTGTGCT GCCCGAAGAT ATGAAGACTC TCTGGGCCAA GAAAGCCTAC
    GGGCTCCAGA GCGAGCTGCA GTACAAGGCT GACCTGGCGT GGATGAAAGG GGTCAGGTGG
    CTGACTGAGG GCAGTCTCAA CTTGGAACAG GCCAAGAAGG CTGGACAGCT GGTCAGCGAG
    AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGGCCGA CAGCTCCCAG
    ATGGAGCATG CCAAGAAGAG CCAGGAACTG CAGAGCGGGG TGGCCTACAA GGCAGAGCAC
    GAGCAGTCCG TCCATCAGTA CAGCATTAGC AAAGACGAGC CTCTCTTCCT ACAGGCCCGA
    GCCAACGCTG CCAATCTCAG CGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCG
    AAAGCATTTG ATCTGCGTCT TGACTCCTTG GCCTTCCTGG CAGCCAAAGC CAAGAGGGAC
    CTGGCCAGTG AGGTGAAGTA CAAAGAAGAT TATGAAAAAT CCAGAGGGAA GCTCATTGGA
    GCTCAGGGTG CCCAGGGGGA TTCGCAAATG AGCCACTCGC TGCAAATGTC CAAGCTCCAG
    AGTGAACTGG AATACAAGAA GGGCTTCGAG GACACCAAGT CTCAGTGCCA TGTCCCCCTG
    GACATGATCC ACCTCGTGCA TGCCCGCAAG GCTCAGCACT TAGCCACAGA CATAGGCTAC
    AAGACGGCAT CCCATCACTT TACAGCTTTG CCCACAGACA TGAAGGTGGA ATGGGCCAAG
    AAGGCTTACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGG
    ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC
    ATTAGCGAGA AGAAGTACCG GCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC
    ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTGTACCGA
    GAGCAAGGAG AAAACCTAAA GCATCACTAC ACGCAGACCA CTGACCTCCC TGAAGTCCTG
    CTGGCCAAGC TGAATGCCAT GAATATCAGC GAGACACGTT ATAAGGAATC CTGGAGCAAA
    CTTCGAGACG GTGGCTATAA GCTGAGACTG GATGCCATTC CATTCCAGGC AGCAAAGGCT
    TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG
    ATGCTCGGCT CCCGGAGCTT GGAAGATGAT ATCAGCCTTG CGCATTCAGT GTACGCGAGC
    TCTCTGCAGA GCCAAGTGAA TTACAAGAAA GACTTTGAAC ACTCAAAGGC GCAGTTCCAT
    CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGACCCT GGCCAGCGAC
    CAGGACTACA GACACCCGCT CCCCCAGTAC ACGTCCTTGG CTGAGGACCT GAGGCTCAGC
    TGCGCCAAGA GAGCTCACAA GTTGCAGAGT GAGAATTTGT ACCGGTCGGA CCTGAACTTT
    ATGCGGGGTG TTGCCTGCGT CATTCCAGGC ACGTTAGAGA TTGAAGGGAG AAAGAGAGCA
    TCTGAACTCA TCAGTGAGAG TAAATATCGT CAGCATCCCC AGTCGCTCAA GTACACAGCC
    GTGACAGACA CACCCAGCCT CACTCATGCA AAACTCAGCA ACCAGATTAC AAACGAGCGC
    CTCTATAAAG CAGCGGGGGA AGATGCGAGA CACCAGTACA CAATGACCCT GGGCCTGCCC
    GAGTTTGTCC GAGCGAAAAC AAACGCGGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT
    TGGAGGAATC TCCATGCTCA AGGCTACAAG CTAACAATAG ATGCTCTGCC CTTCCAGGCG
    GCTCGAGTCT CTGGAGACAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG
    CGAGGACGGC TGATCGGGGC CCAGAGCGTG AGTGACGACC CCCGGCTCCA GCACTGTCAG
    CGGGTGGGCC AGCTGCAGAG CGAGCTTCAG TACAGGAGGC AGGCGGCAGG CAGCAGAGCG
    CAGTGCCACC TGCCCATGGA CATGGTGCCA CTGGTCCATG CCCGGAAGGC CCAGGCCCTG
    GCCAGCGACC TTGACTACAG GACACAGTGC CACGCGTTCA CAGCGCTGCC CGAGGACCTG
    AGGATGGCCT GGGCCAAGAA GGCCCATGCC CTGCAGAGTG AGCTGCGCTA CAAGTCGGAC
    CTGATGGGCA TGAAGGGGAC AGGATGGCTG GCGCTGAGTT CCCCACAGAT AGAAAGTGCA
    AAGAAGGCCG GAGAACTCAT CAGTGAGACC AAATACCGTG AAAAACCAGA CACCATCAAG
    TTTACCACAG TGGTTGACTC CCCGGACCTA GTTCATGCCA AGAACAGCTA CATGCATTGC
    AATGAGCGCC TGTACAGGTC GGGGGATGCG GAATCCCGAC ACAGGTACAC CCTGGTGCCC
    GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCCCTGA ATCTGAGCGA CAAAGTCTAC
    AGACACTCCT GGGAGCAGAC CCGGGCTGGC GGCTACGACT TCAGGCTGGA TGCCATCCCA
    TTCCAGACGG CACGGGCATC GAGGGAAATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC
    CTACGGGACC GGGGCCTGCA GATAGGGTAC CGCAGCATCA ACGACGACCC GAGGACGAAG
    CATTTCCTCA GCGTCGGCAG ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTCGCCAAG
    AGTCGGTCCC AGTTCCACAG CCGCCCAGAC CAGCCCGGCT TCCTCCAGGC CAAGAGGAGC
    CAGCAGCTGG CCAGCGACGT GCACTACAGG CAGCCCCTGC CCCAGCCCAC CTGCGACCCG
    GAGCAGCTGG GCCTGAAACA CGCGCAGAAG GCCCACCGGC TGCAGAGCGA TGTCAAGTAC
    AAATCAGACT TGAACCTGAC CAGAGGTATT GGCTGGACAC CTCCTGGCTC CTACAAAGTG
    GAAATGGCTC GGCGGGCTGC AGAATTGGCC AACGCGAGGG GCCTGGGTCT CCAAGGGGCT
    TACGGGGGGC CGGAGGCTGT GGAGCCCAGA GATGATCAGA GCGGGTTTGT AAACCCAGAT
    GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCCCCAC TGTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.