ANKS1A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_611767.8	XP_611767.6	ankyrin repeat and SAM domain-containing protein 1A isoform X6
XM_005223297.4	XP_005223354.1	ankyrin repeat and SAM domain-containing protein 1A isoform X1
XM_005223297.3	XP_005223354.1	ankyrin repeat and SAM domain-containing protein 1A isoform X1
XM_002697185.5	XP_002697231.3	ankyrin repeat and SAM domain-containing protein 1A isoform X6
XM_005223300.4	XP_005223357.1	ankyrin repeat and SAM domain-containing protein 1A isoform X4
XM_005196560.3	XP_005196617.1	ankyrin repeat and SAM domain-containing protein 1A isoform X7
XM_005196558.3	XP_005196615.1	ankyrin repeat and SAM domain-containing protein 1A isoform X4
XM_005196557.3	XP_005196614.1	ankyrin repeat and SAM domain-containing protein 1A isoform X3
XM_005223302.3	XP_005223359.1	ankyrin repeat and SAM domain-containing protein 1A isoform X7
XM_005223298.3	XP_005223355.1	ankyrin repeat and SAM domain-containing protein 1A isoform X2
XM_005223299.3	XP_005223356.1	ankyrin repeat and SAM domain-containing protein 1A isoform X3
XM_005196555.3	XP_005196612.1	ankyrin repeat and SAM domain-containing protein 1A isoform X1
XM_005196556.3	XP_005196613.1	ankyrin repeat and SAM domain-containing protein 1A isoform X2
XM_005196559.3	XP_005196616.1	ankyrin repeat and SAM domain-containing protein 1A isoform X5
XM_005223301.3	XP_005223358.1	ankyrin repeat and SAM domain-containing protein 1A isoform X5
XM_005223301.4	XP_005223358.1	ankyrin repeat and SAM domain-containing protein 1A isoform X5
XM_024983955.1	XP_024839723.1	ankyrin repeat and SAM domain-containing protein 1A isoform X7
XM_024983956.1	XP_024839724.1	ankyrin repeat and SAM domain-containing protein 1A isoform X8
XM_005223300.3	XP_005223357.1	ankyrin repeat and SAM domain-containing protein 1A isoform X4
XM_002697185.6	XP_002697231.3	ankyrin repeat and SAM domain-containing protein 1A isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa11171	XM_005223302.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa11171	XM_024983956.1 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa11171	XM_005196560.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa229721	XM_024983955.1 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa11165	XM_005196555.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa11165	XM_005223297.4 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa11166	XM_005196556.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa11166	XM_005223298.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa11167	XM_005223299.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa11167	XM_005196557.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa11168	XM_005196558.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa11168	XM_005223300.4 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa11169	XM_005196559.3 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa11169	XM_005223301.4 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa11170	XM_611767.8 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa11170	XM_002697185.6 Latest version!	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OBa11165	XM_005223297.3	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa11169	XM_005223301.3	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa11168	XM_005223300.3	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa11170	XM_002697185.5	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

ANKS1A ( XM_005196560.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196617.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa11171
Clone ID Related Accession (Same CDS sequence)	XM_005196560.3 , XM_005223302.3 , XM_024983956.1
Accession Version	XM_005196560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646560.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196617.1
CDS	175..3168
Translation	MRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005196560.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196560.3

ORF Insert Sequence:

ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223302.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223359.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa11171
Clone ID Related Accession (Same CDS sequence)	XM_005196560.3 , XM_005223302.3 , XM_024983956.1
Accession Version	XM_005223302.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223359.1
CDS	175..3168
Translation	MRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005223302.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223302.3

ORF Insert Sequence:

ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_024983956.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839724.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa11171
Clone ID Related Accession (Same CDS sequence)	XM_005196560.3 , XM_005223302.3 , XM_024983956.1
Accession Version	XM_024983956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839724.1
CDS	224..3217
Translation	MRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_024983956.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983956.1

ORF Insert Sequence:

ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_024983955.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839723.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa229721
Clone ID Related Accession (Same CDS sequence)	XM_024983955.1
Accession Version	XM_024983955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839723.1
CDS	141..3557
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTN

Target ORF information:

RefSeq Version	XM_024983955.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983955.1

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223297.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223354.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.

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CloneID	OBa11165
Clone ID Related Accession (Same CDS sequence)	XM_005223297.4 , XM_005223297.3 , XM_005196555.3
Accession Version	XM_005223297.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223297.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAAGTACG AGACCACTAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223354.1
CDS	141..3737
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005223297.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223297.4

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAAGTACG AGACCACTAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223297.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223354.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.

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CloneID	OBa11165
Clone ID Related Accession (Same CDS sequence)	XM_005223297.4 , XM_005223297.3 , XM_005196555.3
Accession Version	XM_005223297.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAAGTACG AGACCACTAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223354.1
CDS	142..3738
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005223297.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223297.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAAGTACG AGACCACTAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005196555.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196612.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.

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CloneID	OBa11165
Clone ID Related Accession (Same CDS sequence)	XM_005223297.4 , XM_005223297.3 , XM_005196555.3
Accession Version	XM_005196555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646560.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAAGTACG AGACCACTAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196612.1
CDS	142..3738
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005196555.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196555.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAAGTACG AGACCACTAT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223298.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223355.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X2, mRNA.

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CloneID	OBa11166
Clone ID Related Accession (Same CDS sequence)	XM_005223298.3 , XM_005196556.3
Accession Version	XM_005223298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223355.1
CDS	141..3719
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTN

Target ORF information:

RefSeq Version	XM_005223298.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223298.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005196556.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196613.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa11166
Clone ID Related Accession (Same CDS sequence)	XM_005223298.3 , XM_005196556.3
Accession Version	XM_005196556.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646560.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196556.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196613.1
CDS	141..3719
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTN

Target ORF information:

RefSeq Version	XM_005196556.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196556.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGGT GCCGCCGCCC CCCGGTAGTC GCTGTTGTCA CTGTCACACC 
CGCCCCGCCC ACCGTCCTTC CTACCTCCCG CTGCCGTCTG TTAGTCCTGG AGCCAAGCTG 
GAACCACCTG ACACGGACCC CGACGCCCAG TCCCACGCCA GCGTCTCCTG GGTCGTGGAC 
CCCAAGCCAG ACTCTAAGCG GAGCCTCAGC ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005196557.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196614.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X3, mRNA.

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CloneID	OBa11167
Clone ID Related Accession (Same CDS sequence)	XM_005196557.3 , XM_005223299.3
Accession Version	XM_005196557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646560.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196614.1
CDS	141..3674
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005196557.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196557.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223299.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223356.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X3, mRNA.

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CloneID	OBa11167
Clone ID Related Accession (Same CDS sequence)	XM_005196557.3 , XM_005223299.3
Accession Version	XM_005223299.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223299.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223356.1
CDS	141..3674
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSAVPPPPGSRCCHCHTRPAHRPSYLPLPSVSPGAKLEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005223299.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223299.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGGTGCC GCCGCCCCCC GGTAGTCGCT GTTGTCACTG TCACACCCGC 
CCCGCCCACC GTCCTTCCTA CCTCCCGCTG CCGTCTGTTA GTCCTGGAGC CAAGCTGGAA 
CCACCTGACA CGGACCCCGA CGCCCAGTCC CACGCCAGCG TCTCCTGGGT CGTGGACCCC 
AAGCCAGACT CTAAGCGGAG CCTCAGCACC AAGTACGAGA CCACTATCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223300.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223357.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.

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CloneID	OBa11168
Clone ID Related Accession (Same CDS sequence)	XM_005223300.4 , XM_005196558.3 , XM_005223300.3
Accession Version	XM_005223300.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223300.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAAGTAC 
GAGACCACTA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223357.1
CDS	141..3638
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005223300.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223300.4

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAAGTAC 
GAGACCACTA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005196558.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196615.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.

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CloneID	OBa11168
Clone ID Related Accession (Same CDS sequence)	XM_005223300.4 , XM_005196558.3 , XM_005223300.3
Accession Version	XM_005196558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646560.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAAGTAC 
GAGACCACTA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196615.1
CDS	140..3637
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005196558.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196558.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAAGTAC 
GAGACCACTA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223300.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223357.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.

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CloneID	OBa11168
Clone ID Related Accession (Same CDS sequence)	XM_005223300.4 , XM_005196558.3 , XM_005223300.3
Accession Version	XM_005223300.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223300.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAAGTAC 
GAGACCACTA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223357.1
CDS	140..3637
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_005223300.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223300.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAAGTAC 
GAGACCACTA TCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005196559.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196616.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.

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CloneID	OBa11169
Clone ID Related Accession (Same CDS sequence)	XM_005196559.3 , XM_005223301.3 , XM_005223301.4
Accession Version	XM_005196559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646560.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005196559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196616.1
CDS	140..3619
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTN

Target ORF information:

RefSeq Version	XM_005196559.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005196559.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223301.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223358.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.

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CloneID	OBa11169
Clone ID Related Accession (Same CDS sequence)	XM_005196559.3 , XM_005223301.3 , XM_005223301.4
Accession Version	XM_005223301.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223301.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223358.1
CDS	140..3619
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTN

Target ORF information:

RefSeq Version	XM_005223301.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223301.3

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_005223301.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223358.1 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.

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CloneID	OBa11169
Clone ID Related Accession (Same CDS sequence)	XM_005196559.3 , XM_005223301.3 , XM_005223301.4
Accession Version	XM_005223301.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223301.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223358.1
CDS	141..3620
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNALEIKELKKYGPFDPYINAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTN

Target ORF information:

RefSeq Version	XM_005223301.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223301.4

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAT GCTCTTGAGA TCAAAGAACT CAAAAAGTAC 
GGCCCCTTTG ACCCTTATAT CAATGCCAAG AACAATGACA ACGAGACGGC CCTGCACTGC 
GCAGCGCAGT ACGGCCACAC CGAGGTGGTG AAGGTCCTCT TAGAGGAGCT GACTGACCCC 
ACCATGCGCA ACAACAAGTT CGAGACGCCT CTGGACCTGG CCGCACTCTA CGGGAGGCTG 
GAGGTGGTGA AGATGCTCCT CAACGCCCAC CCTAACCTCC TGAGCTGCAA CACTAAGAAG 
CACACCCCTC TGCACCTGGC AGCGAGGAAT GGCCACAAAG CCGTGGTGCA GGTCCTCCTG 
GACGCCGGCA TGGATAGCAG CTACCAGACG GAGAAGGGCA GTGCTTTGCA TGAGGCTGCT 
TTGTTTGGCA AGACCGATGT GGTGCAAATC CTGCTGGCTG CAGGAATTGA TGTCAACATA 
AAGGATAACC GAGGATTGAC CGCACTGGAC ACCGTCCGGG AGCTGCCATC TCAGAAGAGC 
CAGCAGATAG CGGCACTCAT CGAAGATCAC ATGACTGGAA AAAGAAGTGC AAAAGAGGTA 
GACAAAACCC TTACACCCCA GCCACCTCTC ATCTCCAACC TGGACTCTAT ATCCCAGAAG 
TCTCAGGGTG ACCTGGAGAA AGCAGTGACT GAACTGATCA TCGATTTTAA CACCAGCACA 
GAAGAGGAGG GTCCCTATGA GGCGCTGTAC AATGCTGTCT CCTGCCAGTC CCTGGACAGC 
ACGGCCAGTG GACGCTCGTC CGACCGGGAC TCTGTGAGCA GGGAGGCTGA GGCGGCCGGA 
GGGAAAGCTG CTGGAGTGAG GCCTAGGGAA CGTCCTCCGC CTCCAGCAAA ACCACCACCT 
GACGAAGAGG AGGAAGACCA CATAGATAAG AAGTATTTTC CCTTGACAGC TTCTGAGGTC 
TTGGCCATGA GACCCTGGGT TCAGGGGAGC CCAGCCAAGG AGGAGGATGA GCACCCTTAT 
GAGCTGCTGC TGACAGCAGA GACGAAGAAG CTGGTGTCTG TGGATGGAAA GACAAAAGAC 
CACAGGCAGA GCAGCGGTGG CCGGAGCCAG GACTCTGCGG AGGGGCAGGA CGGGCAGGTC 
CCGGAGCAGT TCTCAGGTCT CCTCCACGGC TCCTCCCCGG TGTGTGAGGC GGGGCAGGAC 
CCTCTCCAGC TGCTCCCTGC CCCTGGCCAG AACCATCGAG AAGGGTCCCC CGCGCAGGGC 
GCCTGCCCCG AGGAGATGCA GCTGGAGCAG ACAGGCAGGC GTGCGTCCGG AGCCTCCCCG 
CCCCGTGTGC TGGACCAGAG TAGGAGAGTG GGCCACCCGG CAAGCCTGCC CCCCGCCCAC 
AGCCAGACGC ACCCCAAAAC TTCAATGCGC ACAGCCTTTC CACGCCCTGG GGGCGCCGAG 
GAGGAAGGTG ACCCGAGCGG GGCCAGGAGC CGAGCCCCTC CCACCAGCAG ACCCAAGGCT 
GAGCTCAAAC TCAGCCGCAG CTTGTCCAAG TCTGACTCTG ATCTCCTGAC CTGCTCCCCA 
ACGGAGGACG CCACGATGGG GAGCCGGAGT GAGTCCCTCT CCAACTGCAG CATTGGGAAG 
AAGAGGCTGG AGAAGTCGCC CTCCTTCGCC TCTGAGTGGG ATGAGATTGA GAAGATCATG 
AGCTCTATTG GAGAAGGGAT CGACTTTTCT CAGGAACAGC AGAGGATCTC AGGTGCGCGG 
CCGCTGGAAC AGAGCGTCGG GGAGTGGCTG GAGGCCGTGG GGCTGCAGCA GTATGAGAGC 
AGGCTGCTTC TCAACGGCTT TGACGATGTC CGCTTCCTGG GGCCTAACGT GATGGAAGAT 
CAGGACCTGC GGGAAATCGG CATCAGTGAT GCACAGCACA GGCGGAAGCT GCTCCAGGCC 
GCACGGTCCC TGCCCAAGGT GAAGGCGCTG GGCTACGACG GCAACAGCCC CCTGTCAGTG 
CCCTCCTGGC TGGACTCGCT GGGGCTTCAG GACTATGTCC ATTCCTTCCT GTCGAGTGGC 
TACAGCTCCA TTGACACCGT GAAGAACCTC TGGGAGCTGG AGCTCGTCAA CGTCCTGAAG 
GTGCACCTGC TCGGCCATCG CAAGCGCATC ATCGCCTCCC TGGCGGACAG GCCCTACGAG 
GAGCCGCCCC AGAAGCCCCC GCGGTTCTCC CAGCTGAGAT GCCAGGACCT GTTCTCGCAG 
ACGTCGTCCC CGCTGAGCCA GAACGACTCC TGTGCCGGGC GGTCAGCTGA CCTGCTGCTG 
CCTCCTGGGG ACGCGGGCAG GAGGCCGCAC GACAGCCTGC ACGAGCCTGC GGCGCCCTCT 
CGGGCAGAGC GCTTCAGAAC CCAGGAGGAG CACCGAGAGG CCAAGCTGAC CCTCCGGCCC 
CCCAGCCTGG CTGCCCCCTA CGCCCCTGTG CAGAGCTGGC AACACCAGCC GGAGAAACTC 
ATCTTCGAGT CCTGCGGTTA CGAAGCCAAC TATCTGGGCT CCATGTTGAT CAAAGATCTG 
CGAGGGACAG AATCCACACA AGACGCCTGT GCCAAGATGC GGAAATCCAC AGAGCACATG 
AGGAAGATCC CCACCATCAT CCTGTCCATC ACATACAAAG GGGTCAAGTT CATCGATGCC 
TCCAACAAGA ATGTCATCGC AGAGCACGAG ATCCGGAACA TTTCCTGCGC GGCCCAGGAC 
CCAGAGGACC TGTGCGCCTT TGCCTACGTC ACCAAGGACC TGCAGACTGG CCACCATTAC 
TGCCACGTCT TCAGCACGGT GGATGTGAAC CTGACCTATG AGATCATCCT GACGCTGGGC 
CAGGCGTTCG AGGTGGCCTA CCAGCTGGCC CTGCAGGCCC AGAAGTCCAG GCCGGTGGGG 
GCCTCTGCCG CTGAGGTGAT CGAGACCAGA TCTTCCAAGC CCGTGCCGAA GCCCCGGGTG 
GGCGTGCGGA AGTCAGCGCT GGAACCACCT GACACGGACC CCGACGCCCA GTCCCACGCC 
AGCGTCTCCT GGGTCGTGGA CCCCAAGCCA GACTCTAAGC GGAGCCTCAG CACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_611767.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_611767.6 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.

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CloneID	OBa11170
Clone ID Related Accession (Same CDS sequence)	XM_611767.8 , XM_002697185.5 , XM_002697185.6
Accession Version	XM_611767.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646560.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_611767.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAAGTACGAG 
ACCACTATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_611767.6
CDS	140..3574
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_611767.8
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_611767.8

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAAGTACGAG 
ACCACTATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_002697185.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697231.3 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.

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CloneID	OBa11170
Clone ID Related Accession (Same CDS sequence)	XM_611767.8 , XM_002697185.5 , XM_002697185.6
Accession Version	XM_002697185.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697185.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAAGTACGAG 
ACCACTATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697231.3
CDS	140..3574
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_002697185.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697185.5

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAAGTACGAG 
ACCACTATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKS1A ( XM_002697185.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697231.3 Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa11170
Clone ID Related Accession (Same CDS sequence)	XM_611767.8 , XM_002697185.5 , XM_002697185.6
Accession Version	XM_002697185.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and SAM domain-containing protein 1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697185.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAAGTACGAG 
ACCACTATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697231.3
CDS	141..3575
Translation	MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGGSGGGSGGGSGSGGGGGGLGSSSHALSSLLSIWRGPNVNCVDSTGYTPLHHAALNGHKDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTKVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMDSSYQTEKGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRELPSQKSQQIAALIEDHMTGKRSAKEVDKTLTPQPPLISNLDSISQKSQGDLEKAVTELIIDFNTSTEEEGPYEALYNAVSCQSLDSTASGRSSDRDSVSREAEAAGGKAAGVRPRERPPPPAKPPPDEEEEDHIDKKYFPLTASEVLAMRPWVQGSPAKEEDEHPYELLLTAETKKLVSVDGKTKDHRQSSGGRSQDSAEGQDGQVPEQFSGLLHGSSPVCEAGQDPLQLLPAPGQNHREGSPAQGACPEEMQLEQTGRRASGASPPRVLDQSRRVGHPASLPPAHSQTHPKTSMRTAFPRPGGAEEEGDPSGARSRAPPTSRPKAELKLSRSLSKSDSDLLTCSPTEDATMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQRISGARPLEQSVGEWLEAVGLQQYESRLLLNGFDDVRFLGPNVMEDQDLREIGISDAQHRRKLLQAARSLPKVKALGYDGNSPLSVPSWLDSLGLQDYVHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRCQDLFSQTSSPLSQNDSCAGRSADLLLPPGDAGRRPHDSLHEPAAPSRAERFRTQEEHREAKLTLRPPSLAAPYAPVQSWQHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMRKIPTIILSITYKGVKFIDASNKNVIAEHEIRNISCAAQDPEDLCAFAYVTKDLQTGHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQKSRPVGASAAEVIETRSSKPVPKPRVGVRKSALEPPDTDPDAQSHASVSWVVDPKPDSKRSLSTKYETTIF

Target ORF information:

RefSeq Version	XM_002697185.6
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and sterile alpha motif domain containing 1A (ANKS1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697185.6

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GCTGGAGGCG GCCCGCACCG GACACCTCCC GGCCGTGGAG 
AAGCTGCTGT CCGGGAAGCG GCTCTCCTCA GGCTTCGGGG GCGGCGGCGG CGGCGGCGGC 
TCTGGAGGCG GTAGCGGCGG CGGCAGCGGC TCTGGAGGTG GCGGCGGCGG TCTTGGCTCC 
TCGAGCCATG CCCTGTCCAG TCTGCTCAGC ATTTGGAGAG GGCCAAATGT GAACTGTGTT 
GACAGCACTG GGTACACACC TCTGCACCAT GCTGCTTTGA ATGGCCATAA GGATGTGGTC 
GAGGTTCTTC TGAGGAACGA CGCGCTGACC AACGTGGCTG ACTCTAAAGG CTGTTATCCG 
CTGCACTTGG CAGCCTGGAA AGGAGACGCC CAGATAGTGC GATTGCTCAT CCATCAAGGG 
CCCTCACACA CCAAAGTCAA TGAACAGAAC AATGACAACG AGACGGCCCT GCACTGCGCA 
GCGCAGTACG GCCACACCGA GGTGGTGAAG GTCCTCTTAG AGGAGCTGAC TGACCCCACC 
ATGCGCAACA ACAAGTTCGA GACGCCTCTG GACCTGGCCG CACTCTACGG GAGGCTGGAG 
GTGGTGAAGA TGCTCCTCAA CGCCCACCCT AACCTCCTGA GCTGCAACAC TAAGAAGCAC 
ACCCCTCTGC ACCTGGCAGC GAGGAATGGC CACAAAGCCG TGGTGCAGGT CCTCCTGGAC 
GCCGGCATGG ATAGCAGCTA CCAGACGGAG AAGGGCAGTG CTTTGCATGA GGCTGCTTTG 
TTTGGCAAGA CCGATGTGGT GCAAATCCTG CTGGCTGCAG GAATTGATGT CAACATAAAG 
GATAACCGAG GATTGACCGC ACTGGACACC GTCCGGGAGC TGCCATCTCA GAAGAGCCAG 
CAGATAGCGG CACTCATCGA AGATCACATG ACTGGAAAAA GAAGTGCAAA AGAGGTAGAC 
AAAACCCTTA CACCCCAGCC ACCTCTCATC TCCAACCTGG ACTCTATATC CCAGAAGTCT 
CAGGGTGACC TGGAGAAAGC AGTGACTGAA CTGATCATCG ATTTTAACAC CAGCACAGAA 
GAGGAGGGTC CCTATGAGGC GCTGTACAAT GCTGTCTCCT GCCAGTCCCT GGACAGCACG 
GCCAGTGGAC GCTCGTCCGA CCGGGACTCT GTGAGCAGGG AGGCTGAGGC GGCCGGAGGG 
AAAGCTGCTG GAGTGAGGCC TAGGGAACGT CCTCCGCCTC CAGCAAAACC ACCACCTGAC 
GAAGAGGAGG AAGACCACAT AGATAAGAAG TATTTTCCCT TGACAGCTTC TGAGGTCTTG 
GCCATGAGAC CCTGGGTTCA GGGGAGCCCA GCCAAGGAGG AGGATGAGCA CCCTTATGAG 
CTGCTGCTGA CAGCAGAGAC GAAGAAGCTG GTGTCTGTGG ATGGAAAGAC AAAAGACCAC 
AGGCAGAGCA GCGGTGGCCG GAGCCAGGAC TCTGCGGAGG GGCAGGACGG GCAGGTCCCG 
GAGCAGTTCT CAGGTCTCCT CCACGGCTCC TCCCCGGTGT GTGAGGCGGG GCAGGACCCT 
CTCCAGCTGC TCCCTGCCCC TGGCCAGAAC CATCGAGAAG GGTCCCCCGC GCAGGGCGCC 
TGCCCCGAGG AGATGCAGCT GGAGCAGACA GGCAGGCGTG CGTCCGGAGC CTCCCCGCCC 
CGTGTGCTGG ACCAGAGTAG GAGAGTGGGC CACCCGGCAA GCCTGCCCCC CGCCCACAGC 
CAGACGCACC CCAAAACTTC AATGCGCACA GCCTTTCCAC GCCCTGGGGG CGCCGAGGAG 
GAAGGTGACC CGAGCGGGGC CAGGAGCCGA GCCCCTCCCA CCAGCAGACC CAAGGCTGAG 
CTCAAACTCA GCCGCAGCTT GTCCAAGTCT GACTCTGATC TCCTGACCTG CTCCCCAACG 
GAGGACGCCA CGATGGGGAG CCGGAGTGAG TCCCTCTCCA ACTGCAGCAT TGGGAAGAAG 
AGGCTGGAGA AGTCGCCCTC CTTCGCCTCT GAGTGGGATG AGATTGAGAA GATCATGAGC 
TCTATTGGAG AAGGGATCGA CTTTTCTCAG GAACAGCAGA GGATCTCAGG TGCGCGGCCG 
CTGGAACAGA GCGTCGGGGA GTGGCTGGAG GCCGTGGGGC TGCAGCAGTA TGAGAGCAGG 
CTGCTTCTCA ACGGCTTTGA CGATGTCCGC TTCCTGGGGC CTAACGTGAT GGAAGATCAG 
GACCTGCGGG AAATCGGCAT CAGTGATGCA CAGCACAGGC GGAAGCTGCT CCAGGCCGCA 
CGGTCCCTGC CCAAGGTGAA GGCGCTGGGC TACGACGGCA ACAGCCCCCT GTCAGTGCCC 
TCCTGGCTGG ACTCGCTGGG GCTTCAGGAC TATGTCCATT CCTTCCTGTC GAGTGGCTAC 
AGCTCCATTG ACACCGTGAA GAACCTCTGG GAGCTGGAGC TCGTCAACGT CCTGAAGGTG 
CACCTGCTCG GCCATCGCAA GCGCATCATC GCCTCCCTGG CGGACAGGCC CTACGAGGAG 
CCGCCCCAGA AGCCCCCGCG GTTCTCCCAG CTGAGATGCC AGGACCTGTT CTCGCAGACG 
TCGTCCCCGC TGAGCCAGAA CGACTCCTGT GCCGGGCGGT CAGCTGACCT GCTGCTGCCT 
CCTGGGGACG CGGGCAGGAG GCCGCACGAC AGCCTGCACG AGCCTGCGGC GCCCTCTCGG 
GCAGAGCGCT TCAGAACCCA GGAGGAGCAC CGAGAGGCCA AGCTGACCCT CCGGCCCCCC 
AGCCTGGCTG CCCCCTACGC CCCTGTGCAG AGCTGGCAAC ACCAGCCGGA GAAACTCATC 
TTCGAGTCCT GCGGTTACGA AGCCAACTAT CTGGGCTCCA TGTTGATCAA AGATCTGCGA 
GGGACAGAAT CCACACAAGA CGCCTGTGCC AAGATGCGGA AATCCACAGA GCACATGAGG 
AAGATCCCCA CCATCATCCT GTCCATCACA TACAAAGGGG TCAAGTTCAT CGATGCCTCC 
AACAAGAATG TCATCGCAGA GCACGAGATC CGGAACATTT CCTGCGCGGC CCAGGACCCA 
GAGGACCTGT GCGCCTTTGC CTACGTCACC AAGGACCTGC AGACTGGCCA CCATTACTGC 
CACGTCTTCA GCACGGTGGA TGTGAACCTG ACCTATGAGA TCATCCTGAC GCTGGGCCAG 
GCGTTCGAGG TGGCCTACCA GCTGGCCCTG CAGGCCCAGA AGTCCAGGCC GGTGGGGGCC 
TCTGCCGCTG AGGTGATCGA GACCAGATCT TCCAAGCCCG TGCCGAAGCC CCGGGTGGGC 
GTGCGGAAGT CAGCGCTGGA ACCACCTGAC ACGGACCCCG ACGCCCAGTC CCACGCCAGC 
GTCTCCTGGG TCGTGGACCC CAAGCCAGAC TCTAAGCGGA GCCTCAGCAC CAAGTACGAG 
ACCACTATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ANKS1A
Entrez Gene ID	532631
Full Name	ankyrin repeat and sterile alpha motif domain containing 1A
General protein information	Preferred Names ankyrin repeat and sterile alpha motif domain containing 1A Names ankyrin repeat and SAM domain-containing protein 1A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Xenopus tropicalis (tropical clawed frog)	anks1a	NP_001106480.1
Pan troglodytes (chimpanzee)	ANKS1A	XP_518420.3
Mus musculus (house mouse)	Anks1	NP_001272969.1
Rattus norvegicus (Norway rat)	Anks1a	NP_001101083.1
Canis lupus familiaris (dog)	ANKS1A	XP_005627295.1
Bos taurus (cattle)	ANKS1A	XP_005223354.1
Gallus gallus (chicken)	ANKS1A	XP_004935094.1
Homo sapiens (human)	ANKS1A	NP_056060.2
	ANKS1A	XP_001111692.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ANKS1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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