GIGYF1 cDNA ORF clone, Bos taurus(cattle)

The following GIGYF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIGYF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa230328 XM_024984971.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa33276 NM_001205998.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa33274 XM_024984967.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa33274 XM_010819566.2
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa33275 XM_024984968.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa33275 XM_024984969.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa33275 XM_010819577.2
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa33277 XM_024984970.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460478.2
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460477.2
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460479.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460480.2
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460473.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460474.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460475.2
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460476.2
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88575 XM_015460481.1
Latest version!
Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OBa88574 XM_015460476.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460477.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460480.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460475.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa88574 XM_015460478.1 Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa230328
    Clone ID Related Accession (Same CDS sequence) XM_024984971.1
    Accession Version XM_024984971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG
    CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCC GCCAGCTCCC GCAGTGCGTG
    CTCCGGGAAA AGGCAGCTCT GGGAGACCTG CCGCCGCCGC AGCAGCAGCA GCTCACCGCG
    TTCCTGCAGC AGCTCCAAGC TCTCAAACCC CCCAGGGGCG GGGACCAGAA CTTGCTCCCG
    ACGATGAACC GGTCCTTGTC GGTGCCAGAC TCGGGTCCCC TCTGGGACAT ACATACCTCA
    GCCTCACCAC AGTCAGGCGG TGAGGCCAGT CTTTGGGATA TACCAATTAA CTCTTCGACT
    CAGGGTCCAA TTCTAGAACA ACTCCAGCTG CAACACAAAT TCCAGGAGCG CAGAGAAGTG
    GAGCTCAGGG CGAAGCGGGA GGAGGAGGAG CGGAAGCGCC GGGAAGAGAA GCGCCGCCAG
    CAGCAGCAGC AGCAGGAGGA GCAGAAGCGG CGGCAGGAGG AAGAGGAGCT GTTCCGGCGC
    AAGCAGGCGC GGCAGCAGGA GCTGCTGCTG AAGCTGCTGC AGCAGCAGCA GCAGGCGGTG
    GCCGCCGTCC CCGCGCCTCC TACACCCAGT TCCCCGCCTC CGCTGTGGGC TGGCCTGGCC
    AAGCAGGGCC TGTCCATGAA GACGCTGCTG GAGCTGCAGC TGGAGGGCGA GCGGCACCTG
    CATAAGCAGC CCCCGCCTCG GGAGCCATCG CGGGCCCAGG CCCCCAACCA CCGCGTGCAG
    CTCGGGGGCC TGGGCTCCGC CCCCCTGAAC CAGTGGGTAT CTGAGGCCGG GCCGCTGTGG
    GGTGGGCCCG ACAAGAGTGG GGGCGGCAGC AGCAGCCTGG GCCTCTGGGA AGACACCCTC
    AAGAGCAGTG GGAGCCTGGC CCGGAGCCTG GGCCTGAAGA ACAGCCGCAG CAGCCCCTCT
    CTCAGTGACT CGTACAGCCA CTTGTCTGGT CGGCCTGTGC GCAAAAAGAC GGAGGAGGAA
    GAGAAGCTGC TGAAGCTGCT GCAGGGCATC CCCCGGCCCC AGGATGGCTT CACCCAGTGG
    TGTGAGCAGA TGCTGCACAC GCTGAGCACC ACGGGCAGCC TGGACGTGCC CATGGCTGTA
    GCGATCCTCA AGGAGGTGGA ATCCCCCTAT GACGTCCACG ACTATATTCG TTCCTGCCTG
    GGGGACACGC TGGAAGCCAA AGAATTTGCC AAACAATTCC TGGAGCGGAG GGCCAAGCAG
    AAGGCCAGCC AGCAGCGGCA GCAGCAGCAG GAGGCTTGGC TGAGCAGCGG CTCCCTACAG
    ACAGCCTTTC AGACCAACCA CAGCACCAAG CTTGGCCCCG GGGAGGGCAG CAAGGCCAAG
    AGGCGGGCAC TGATGCTGCA CTCGGACCCC AGCATCTTGG GGTACTCCCT GCACGGACCT
    TCTGGTGAGA TCGAGAGCGT GGATGACTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840739.1
    CDS661..3753
    Translation

    Target ORF information:

    RefSeq Version XM_024984971.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG
    CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCC GCCAGCTCCC GCAGTGCGTG
    CTCCGGGAAA AGGCAGCTCT GGGAGACCTG CCGCCGCCGC AGCAGCAGCA GCTCACCGCG
    TTCCTGCAGC AGCTCCAAGC TCTCAAACCC CCCAGGGGCG GGGACCAGAA CTTGCTCCCG
    ACGATGAACC GGTCCTTGTC GGTGCCAGAC TCGGGTCCCC TCTGGGACAT ACATACCTCA
    GCCTCACCAC AGTCAGGCGG TGAGGCCAGT CTTTGGGATA TACCAATTAA CTCTTCGACT
    CAGGGTCCAA TTCTAGAACA ACTCCAGCTG CAACACAAAT TCCAGGAGCG CAGAGAAGTG
    GAGCTCAGGG CGAAGCGGGA GGAGGAGGAG CGGAAGCGCC GGGAAGAGAA GCGCCGCCAG
    CAGCAGCAGC AGCAGGAGGA GCAGAAGCGG CGGCAGGAGG AAGAGGAGCT GTTCCGGCGC
    AAGCAGGCGC GGCAGCAGGA GCTGCTGCTG AAGCTGCTGC AGCAGCAGCA GCAGGCGGTG
    GCCGCCGTCC CCGCGCCTCC TACACCCAGT TCCCCGCCTC CGCTGTGGGC TGGCCTGGCC
    AAGCAGGGCC TGTCCATGAA GACGCTGCTG GAGCTGCAGC TGGAGGGCGA GCGGCACCTG
    CATAAGCAGC CCCCGCCTCG GGAGCCATCG CGGGCCCAGG CCCCCAACCA CCGCGTGCAG
    CTCGGGGGCC TGGGCTCCGC CCCCCTGAAC CAGTGGGTAT CTGAGGCCGG GCCGCTGTGG
    GGTGGGCCCG ACAAGAGTGG GGGCGGCAGC AGCAGCCTGG GCCTCTGGGA AGACACCCTC
    AAGAGCAGTG GGAGCCTGGC CCGGAGCCTG GGCCTGAAGA ACAGCCGCAG CAGCCCCTCT
    CTCAGTGACT CGTACAGCCA CTTGTCTGGT CGGCCTGTGC GCAAAAAGAC GGAGGAGGAA
    GAGAAGCTGC TGAAGCTGCT GCAGGGCATC CCCCGGCCCC AGGATGGCTT CACCCAGTGG
    TGTGAGCAGA TGCTGCACAC GCTGAGCACC ACGGGCAGCC TGGACGTGCC CATGGCTGTA
    GCGATCCTCA AGGAGGTGGA ATCCCCCTAT GACGTCCACG ACTATATTCG TTCCTGCCTG
    GGGGACACGC TGGAAGCCAA AGAATTTGCC AAACAATTCC TGGAGCGGAG GGCCAAGCAG
    AAGGCCAGCC AGCAGCGGCA GCAGCAGCAG GAGGCTTGGC TGAGCAGCGG CTCCCTACAG
    ACAGCCTTTC AGACCAACCA CAGCACCAAG CTTGGCCCCG GGGAGGGCAG CAAGGCCAAG
    AGGCGGGCAC TGATGCTGCA CTCGGACCCC AGCATCTTGG GGTACTCCCT GCACGGACCT
    TCTGGTGAGA TCGAGAGCGT GGATGACTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33276
    Clone ID Related Accession (Same CDS sequence) NM_001205998.1
    Accession Version NM_001205998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Apr 20, 2011 this sequence version replaced XM_610923.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGAGGAC
    ATGAGGGGAA TGCAGCTAAG TCCTGGGGCG GGCTCACCGC CTGGCCCACC AGACCTGGAA
    GATGATGAAG GCTTGAAGCA CCTGCAGCAG GAGGCGGAGA AGCTCGTGGC CTCCCTGCAG
    GACAGCTCCC TGGAGGAGGA GCAGTTCACG GCCGCCATCC AGGCCCAGGG CCTGCGTCAC
    TCTGCAGCCG CCACTGCCCT CCCCCTCAGC CATGGTGCGG CCCGGAAGTG GTTCTACAAG
    GACCCGCAGG GCGAGATCCA AGGCCCCTTC ACGACCCAGG AGATGGCGGA GTGGTTCCAG
    GCGGGCTACT TCTCCATGGC CCTGCTGGTG AAGCGGGGCT GTGATGAGGG CTTCCAGCCA
    CTGGGTGAGG TGATCAAGAT GTGGGGTCGC GTACCCTTTG CCCCTGGGCC CTCACCTCCC
    CCGCTGCTGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC
    CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG
    CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG
    AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC
    ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT
    AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG
    CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG
    AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG
    CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG
    CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG
    GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC
    GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC
    CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC
    GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG
    GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC
    AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG
    ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC
    TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG
    CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT
    CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG
    AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC
    GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC
    AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC
    CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192927.1
    CDS1..3105
    Translation

    Target ORF information:

    RefSeq Version NM_001205998.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGAGGAC
    ATGAGGGGAA TGCAGCTAAG TCCTGGGGCG GGCTCACCGC CTGGCCCACC AGACCTGGAA
    GATGATGAAG GCTTGAAGCA CCTGCAGCAG GAGGCGGAGA AGCTCGTGGC CTCCCTGCAG
    GACAGCTCCC TGGAGGAGGA GCAGTTCACG GCCGCCATCC AGGCCCAGGG CCTGCGTCAC
    TCTGCAGCCG CCACTGCCCT CCCCCTCAGC CATGGTGCGG CCCGGAAGTG GTTCTACAAG
    GACCCGCAGG GCGAGATCCA AGGCCCCTTC ACGACCCAGG AGATGGCGGA GTGGTTCCAG
    GCGGGCTACT TCTCCATGGC CCTGCTGGTG AAGCGGGGCT GTGATGAGGG CTTCCAGCCA
    CTGGGTGAGG TGATCAAGAT GTGGGGTCGC GTACCCTTTG CCCCTGGGCC CTCACCTCCC
    CCGCTGCTGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC
    CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG
    CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG
    AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC
    ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT
    AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG
    CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG
    AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG
    CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG
    CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG
    GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC
    GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC
    CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC
    GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG
    GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC
    AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG
    ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC
    TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG
    CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT
    CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG
    AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC
    GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC
    AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC
    CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33274
    Clone ID Related Accession (Same CDS sequence) XM_010819566.2 , XM_024984967.1
    Accession Version XM_010819566.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC
    CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG
    CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG
    GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC
    TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA
    CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC
    CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG
    GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA
    GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG
    CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG
    CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG
    GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC
    CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT
    GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC
    CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC
    AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC
    AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG
    GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG
    GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC
    TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG
    GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG
    AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG
    GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG
    TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817868.1
    CDS1690..4800
    Translation

    Target ORF information:

    RefSeq Version XM_010819566.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819566.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC
    CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG
    CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG
    GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC
    TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA
    CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC
    CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG
    GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA
    GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG
    CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG
    CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG
    GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC
    CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT
    GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC
    CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC
    AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC
    AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG
    GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG
    GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC
    TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG
    GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG
    AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG
    GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG
    TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33274
    Clone ID Related Accession (Same CDS sequence) XM_010819566.2 , XM_024984967.1
    Accession Version XM_024984967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC
    CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG
    CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG
    GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC
    TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA
    CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC
    CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG
    GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA
    GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG
    CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG
    CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG
    GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC
    CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT
    GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC
    CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC
    AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC
    AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG
    GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG
    GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC
    TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG
    GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG
    AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG
    GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG
    TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840735.1
    CDS661..3771
    Translation

    Target ORF information:

    RefSeq Version XM_024984967.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCAGCCGC
    CAGCTCCCGC AGTGCGTGCT CCGGGAAAAG GCAGCTCTGG GAGACCTGCC GCCGCCGCAG
    CAGCAGCAGC TCACCGCGTT CCTGCAGCAG CTCCAAGCTC TCAAACCCCC CAGGGGCGGG
    GACCAGAACT TGCTCCCGAC GATGAACCGG TCCTTGTCGG TGCCAGACTC GGGTCCCCTC
    TGGGACATAC ATACCTCAGC CTCACCACAG TCAGGCGGTG AGGCCAGTCT TTGGGATATA
    CCAATTAACT CTTCGACTCA GGGTCCAATT CTAGAACAAC TCCAGCTGCA ACACAAATTC
    CAGGAGCGCA GAGAAGTGGA GCTCAGGGCG AAGCGGGAGG AGGAGGAGCG GAAGCGCCGG
    GAAGAGAAGC GCCGCCAGCA GCAGCAGCAG CAGGAGGAGC AGAAGCGGCG GCAGGAGGAA
    GAGGAGCTGT TCCGGCGCAA GCAGGCGCGG CAGCAGGAGC TGCTGCTGAA GCTGCTGCAG
    CAGCAGCAGC AGGCGGTGGC CGCCGTCCCC GCGCCTCCTA CACCCAGTTC CCCGCCTCCG
    CTGTGGGCTG GCCTGGCCAA GCAGGGCCTG TCCATGAAGA CGCTGCTGGA GCTGCAGCTG
    GAGGGCGAGC GGCACCTGCA TAAGCAGCCC CCGCCTCGGG AGCCATCGCG GGCCCAGGCC
    CCCAACCACC GCGTGCAGCT CGGGGGCCTG GGCTCCGCCC CCCTGAACCA GTGGGTATCT
    GAGGCCGGGC CGCTGTGGGG TGGGCCCGAC AAGAGTGGGG GCGGCAGCAG CAGCCTGGGC
    CTCTGGGAAG ACACCCTCAA GAGCAGTGGG AGCCTGGCCC GGAGCCTGGG CCTGAAGAAC
    AGCCGCAGCA GCCCCTCTCT CAGTGACTCG TACAGCCACT TGTCTGGTCG GCCTGTGCGC
    AAAAAGACGG AGGAGGAAGA GAAGCTGCTG AAGCTGCTGC AGGGCATCCC CCGGCCCCAG
    GATGGCTTCA CCCAGTGGTG TGAGCAGATG CTGCACACGC TGAGCACCAC GGGCAGCCTG
    GACGTGCCCA TGGCTGTAGC GATCCTCAAG GAGGTGGAAT CCCCCTATGA CGTCCACGAC
    TATATTCGTT CCTGCCTGGG GGACACGCTG GAAGCCAAAG AATTTGCCAA ACAATTCCTG
    GAGCGGAGGG CCAAGCAGAA GGCCAGCCAG CAGCGGCAGC AGCAGCAGGA GGCTTGGCTG
    AGCAGCGGCT CCCTACAGAC AGCCTTTCAG ACCAACCACA GCACCAAGCT TGGCCCCGGG
    GAGGGCAGCA AGGCCAAGAG GCGGGCACTG ATGCTGCACT CGGACCCCAG CATCTTGGGG
    TACTCCCTGC ACGGACCTTC TGGTGAGATC GAGAGCGTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33275
    Clone ID Related Accession (Same CDS sequence) XM_010819577.2 , XM_024984969.1 , XM_024984968.1
    Accession Version XM_010819577.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817879.1
    CDS1690..4797
    Translation

    Target ORF information:

    RefSeq Version XM_010819577.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819577.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33275
    Clone ID Related Accession (Same CDS sequence) XM_010819577.2 , XM_024984969.1 , XM_024984968.1
    Accession Version XM_024984969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840737.1
    CDS544..3651
    Translation

    Target ORF information:

    RefSeq Version XM_024984969.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33275
    Clone ID Related Accession (Same CDS sequence) XM_010819577.2 , XM_024984969.1 , XM_024984968.1
    Accession Version XM_024984968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840736.1
    CDS661..3768
    Translation

    Target ORF information:

    RefSeq Version XM_024984968.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGGAAACAT GGACCAGGAG CGGCTGAAGA AGCAACAGGA GCTGGCAGCA
    GCCGCCTTGT ACCAGCAGCT GCAGCACCAG CAGTTTCTTC AGCTGGTCGG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33277
    Clone ID Related Accession (Same CDS sequence) XM_024984970.1
    Accession Version XM_024984970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG
    CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCA GCCGCCAGCT CCCGCAGTGC
    GTGCTCCGGG AAAAGGCAGC TCTGGGAGAC CTGCCGCCGC CGCAGCAGCA GCAGCTCACC
    GCGTTCCTGC AGCAGCTCCA AGCTCTCAAA CCCCCCAGGG GCGGGGACCA GAACTTGCTC
    CCGACGATGA ACCGGTCCTT GTCGGTGCCA GACTCGGGTC CCCTCTGGGA CATACATACC
    TCAGCCTCAC CACAGTCAGG CGGTGAGGCC AGTCTTTGGG ATATACCAAT TAACTCTTCG
    ACTCAGGGTC CAATTCTAGA ACAACTCCAG CTGCAACACA AATTCCAGGA GCGCAGAGAA
    GTGGAGCTCA GGGCGAAGCG GGAGGAGGAG GAGCGGAAGC GCCGGGAAGA GAAGCGCCGC
    CAGCAGCAGC AGCAGCAGGA GGAGCAGAAG CGGCGGCAGG AGGAAGAGGA GCTGTTCCGG
    CGCAAGCAGG CGCGGCAGCA GGAGCTGCTG CTGAAGCTGC TGCAGCAGCA GCAGCAGGCG
    GTGGCCGCCG TCCCCGCGCC TCCTACACCC AGTTCCCCGC CTCCGCTGTG GGCTGGCCTG
    GCCAAGCAGG GCCTGTCCAT GAAGACGCTG CTGGAGCTGC AGCTGGAGGG CGAGCGGCAC
    CTGCATAAGC AGCCCCCGCC TCGGGAGCCA TCGCGGGCCC AGGCCCCCAA CCACCGCGTG
    CAGCTCGGGG GCCTGGGCTC CGCCCCCCTG AACCAGTGGG TATCTGAGGC CGGGCCGCTG
    TGGGGTGGGC CCGACAAGAG TGGGGGCGGC AGCAGCAGCC TGGGCCTCTG GGAAGACACC
    CTCAAGAGCA GTGGGAGCCT GGCCCGGAGC CTGGGCCTGA AGAACAGCCG CAGCAGCCCC
    TCTCTCAGTG ACTCGTACAG CCACTTGTCT GGTCGGCCTG TGCGCAAAAA GACGGAGGAG
    GAAGAGAAGC TGCTGAAGCT GCTGCAGGGC ATCCCCCGGC CCCAGGATGG CTTCACCCAG
    TGGTGTGAGC AGATGCTGCA CACGCTGAGC ACCACGGGCA GCCTGGACGT GCCCATGGCT
    GTAGCGATCC TCAAGGAGGT GGAATCCCCC TATGACGTCC ACGACTATAT TCGTTCCTGC
    CTGGGGGACA CGCTGGAAGC CAAAGAATTT GCCAAACAAT TCCTGGAGCG GAGGGCCAAG
    CAGAAGGCCA GCCAGCAGCG GCAGCAGCAG CAGGAGGCTT GGCTGAGCAG CGGCTCCCTA
    CAGACAGCCT TTCAGACCAA CCACAGCACC AAGCTTGGCC CCGGGGAGGG CAGCAAGGCC
    AAGAGGCGGG CACTGATGCT GCACTCGGAC CCCAGCATCT TGGGGTACTC CCTGCACGGA
    CCTTCTGGTG AGATCGAGAG CGTGGATGAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840738.1
    CDS661..3756
    Translation

    Target ORF information:

    RefSeq Version XM_024984970.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGAGCGGCT GAAGAAGCAA CAGGAGCTGG CAGCAGCCGC CTTGTACCAG
    CAGCTGCAGC ACCAGCAGTT TCTTCAGCTG GTCGGCAGCA GCCGCCAGCT CCCGCAGTGC
    GTGCTCCGGG AAAAGGCAGC TCTGGGAGAC CTGCCGCCGC CGCAGCAGCA GCAGCTCACC
    GCGTTCCTGC AGCAGCTCCA AGCTCTCAAA CCCCCCAGGG GCGGGGACCA GAACTTGCTC
    CCGACGATGA ACCGGTCCTT GTCGGTGCCA GACTCGGGTC CCCTCTGGGA CATACATACC
    TCAGCCTCAC CACAGTCAGG CGGTGAGGCC AGTCTTTGGG ATATACCAAT TAACTCTTCG
    ACTCAGGGTC CAATTCTAGA ACAACTCCAG CTGCAACACA AATTCCAGGA GCGCAGAGAA
    GTGGAGCTCA GGGCGAAGCG GGAGGAGGAG GAGCGGAAGC GCCGGGAAGA GAAGCGCCGC
    CAGCAGCAGC AGCAGCAGGA GGAGCAGAAG CGGCGGCAGG AGGAAGAGGA GCTGTTCCGG
    CGCAAGCAGG CGCGGCAGCA GGAGCTGCTG CTGAAGCTGC TGCAGCAGCA GCAGCAGGCG
    GTGGCCGCCG TCCCCGCGCC TCCTACACCC AGTTCCCCGC CTCCGCTGTG GGCTGGCCTG
    GCCAAGCAGG GCCTGTCCAT GAAGACGCTG CTGGAGCTGC AGCTGGAGGG CGAGCGGCAC
    CTGCATAAGC AGCCCCCGCC TCGGGAGCCA TCGCGGGCCC AGGCCCCCAA CCACCGCGTG
    CAGCTCGGGG GCCTGGGCTC CGCCCCCCTG AACCAGTGGG TATCTGAGGC CGGGCCGCTG
    TGGGGTGGGC CCGACAAGAG TGGGGGCGGC AGCAGCAGCC TGGGCCTCTG GGAAGACACC
    CTCAAGAGCA GTGGGAGCCT GGCCCGGAGC CTGGGCCTGA AGAACAGCCG CAGCAGCCCC
    TCTCTCAGTG ACTCGTACAG CCACTTGTCT GGTCGGCCTG TGCGCAAAAA GACGGAGGAG
    GAAGAGAAGC TGCTGAAGCT GCTGCAGGGC ATCCCCCGGC CCCAGGATGG CTTCACCCAG
    TGGTGTGAGC AGATGCTGCA CACGCTGAGC ACCACGGGCA GCCTGGACGT GCCCATGGCT
    GTAGCGATCC TCAAGGAGGT GGAATCCCCC TATGACGTCC ACGACTATAT TCGTTCCTGC
    CTGGGGGACA CGCTGGAAGC CAAAGAATTT GCCAAACAAT TCCTGGAGCG GAGGGCCAAG
    CAGAAGGCCA GCCAGCAGCG GCAGCAGCAG CAGGAGGCTT GGCTGAGCAG CGGCTCCCTA
    CAGACAGCCT TTCAGACCAA CCACAGCACC AAGCTTGGCC CCGGGGAGGG CAGCAAGGCC
    AAGAGGCGGG CACTGATGCT GCACTCGGAC CCCAGCATCT TGGGGTACTC CCTGCACGGA
    CCTTCTGGTG AGATCGAGAG CGTGGATGAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460477.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315963.1
    CDS1507..4734
    Translation

    Target ORF information:

    RefSeq Version XM_015460477.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460476.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315962.1
    CDS1426..4653
    Translation

    Target ORF information:

    RefSeq Version XM_015460476.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460476.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460480.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315966.1
    CDS351..3578
    Translation

    Target ORF information:

    RefSeq Version XM_015460480.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315960.1
    CDS994..4221
    Translation

    Target ORF information:

    RefSeq Version XM_015460474.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460476.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315962.1
    CDS1441..4668
    Translation

    Target ORF information:

    RefSeq Version XM_015460476.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460477.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315963.1
    CDS1492..4719
    Translation

    Target ORF information:

    RefSeq Version XM_015460477.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460477.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315959.1
    CDS1489..4716
    Translation

    Target ORF information:

    RefSeq Version XM_015460473.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460478.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315964.1
    CDS471..3698
    Translation

    Target ORF information:

    RefSeq Version XM_015460478.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460479.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315965.1
    CDS680..3907
    Translation

    Target ORF information:

    RefSeq Version XM_015460479.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460479.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460480.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315966.1
    CDS336..3563
    Translation

    Target ORF information:

    RefSeq Version XM_015460480.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460475.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315961.1
    CDS1506..4733
    Translation

    Target ORF information:

    RefSeq Version XM_015460475.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460475.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460475.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315961.1
    CDS1521..4748
    Translation

    Target ORF information:

    RefSeq Version XM_015460475.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88574
    Clone ID Related Accession (Same CDS sequence) XM_015460477.1 , XM_015460476.2 , XM_015460480.2 , XM_015460474.1 , XM_015460476.1 , XM_015460477.2 , XM_015460473.1 , XM_015460478.1 , XM_015460479.1 , XM_015460480.1 , XM_015460475.2 , XM_015460475.1 , XM_015460478.2
    Accession Version XM_015460478.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GRB10-interacting GYF protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315964.1
    CDS486..3713
    Translation

    Target ORF information:

    RefSeq Version XM_015460478.2
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460478.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CAGCCGCCAG
    CTCCCGCAGT GCGTGCTCCG GGAAAAGGCA GCTCTGGGAG ACCTGCCGCC GCCGCAGCAG
    CAGCAGCTCA CCGCGTTCCT GCAGCAGCTC CAAGCTCTCA AACCCCCCAG GGGCGGGGAC
    CAGAACTTGC TCCCGACGAT GAACCGGTCC TTGTCGGTGC CAGACTCGGG TCCCCTCTGG
    GACATACATA CCTCAGCCTC ACCACAGTCA GGCGGTGAGG CCAGTCTTTG GGATATACCA
    ATTAACTCTT CGACTCAGGG TCCAATTCTA GAACAACTCC AGCTGCAACA CAAATTCCAG
    GAGCGCAGAG AAGTGGAGCT CAGGGCGAAG CGGGAGGAGG AGGAGCGGAA GCGCCGGGAA
    GAGAAGCGCC GCCAGCAGCA GCAGCAGCAG GAGGAGCAGA AGCGGCGGCA GGAGGAAGAG
    GAGCTGTTCC GGCGCAAGCA GGCGCGGCAG CAGGAGCTGC TGCTGAAGCT GCTGCAGCAG
    CAGCAGCAGG CGGTGGCCGC CGTCCCCGCG CCTCCTACAC CCAGTTCCCC GCCTCCGCTG
    TGGGCTGGCC TGGCCAAGCA GGGCCTGTCC ATGAAGACGC TGCTGGAGCT GCAGCTGGAG
    GGCGAGCGGC ACCTGCATAA GCAGCCCCCG CCTCGGGAGC CATCGCGGGC CCAGGCCCCC
    AACCACCGCG TGCAGCTCGG GGGCCTGGGC TCCGCCCCCC TGAACCAGTG GGTATCTGAG
    GCCGGGCCGC TGTGGGGTGG GCCCGACAAG AGTGGGGGCG GCAGCAGCAG CCTGGGCCTC
    TGGGAAGACA CCCTCAAGAG CAGTGGGAGC CTGGCCCGGA GCCTGGGCCT GAAGAACAGC
    CGCAGCAGCC CCTCTCTCAG TGACTCGTAC AGCCACTTGT CTGGTCGGCC TGTGCGCAAA
    AAGACGGAGG AGGAAGAGAA GCTGCTGAAG CTGCTGCAGG GCATCCCCCG GCCCCAGGAT
    GGCTTCACCC AGTGGTGTGA GCAGATGCTG CACACGCTGA GCACCACGGG CAGCCTGGAC
    GTGCCCATGG CTGTAGCGAT CCTCAAGGAG GTGGAATCCC CCTATGACGT CCACGACTAT
    ATTCGTTCCT GCCTGGGGGA CACGCTGGAA GCCAAAGAAT TTGCCAAACA ATTCCTGGAG
    CGGAGGGCCA AGCAGAAGGC CAGCCAGCAG CGGCAGCAGC AGCAGGAGGC TTGGCTGAGC
    AGCGGCTCCC TACAGACAGC CTTTCAGACC AACCACAGCA CCAAGCTTGG CCCCGGGGAG
    GGCAGCAAGG CCAAGAGGCG GGCACTGATG CTGCACTCGG ACCCCAGCAT CTTGGGGTAC
    TCCCTGCACG GACCTTCTGG TGAGATCGAG AGCGTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88575
    Clone ID Related Accession (Same CDS sequence) XM_015460481.1
    Accession Version XM_015460481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product PERQ amino acid-rich with GYF domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC
    CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG
    CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG
    AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC
    ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT
    AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG
    CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG
    AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG
    CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG
    CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG
    GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC
    GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC
    CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC
    GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG
    GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC
    AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG
    ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC
    TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG
    CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT
    CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG
    AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC
    GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC
    AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC
    CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315967.1
    CDS1690..4914
    Translation

    Target ORF information:

    RefSeq Version XM_015460481.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GRB10 interacting GYF protein 1 (GIGYF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGCGG AGACCCTCAA CTTTGGGCCT GAGTGGCTGA GGGCTCTTTC CAGTGGTGGC 
    AGTGTGGCCT CCCCACCCCC GTCCCCCGCC ATGCCCAAGT ACAAGCTGGC CGACTATCGC
    TATGGGCGCG AGGAGATGCT GGCCCTCTAC GTCAAGGAGA GCAAGGTCCC TGACGAGCTG
    CAGGACAAGG AGTTTGCTGC GTTGCTGCAG GAGGAGCCAC TGCAGCCCCT CGCGCTGGAG
    CCGCTGACTG AGGAGGAGCA GAGAAACTTC TCCCTGTCAG TGAACAGCGT GGCCGTGCTG
    AGGCTGATGG GGAAAGGGGC TGGGCCCCCC CTAGGTGGCG CCTCCCGTGG CAGGGGCAGC
    ACACGGAGCC GAGGCCGTGG CCGTGGTGAC AGTTGCTTTT ACCAAAGAAG CATTGAAGAA
    GGTGATGGGG CCTTTGGCCG AAACCCCCGG GAGATCCAGC GTAGCCAGAG TTGGGATGAC
    AGAGGCGAGA GGCGGTTTGA GAAATCGGCC AGGAGAGATG GAGCACGCTC CGGGTTTGAT
    GAGGGAGGGG CTGGCCCGAG GAAGGAGCAC GCCCGCTCAG ATAGCGAAAA TTGGCGTTCT
    CTCCGAGAGG AGCAAGAGGA AGAAGAGGAA GGCAGCTGGA GACTCGGGGC AGGACCCCGG
    CGAGACGGTG ACCGCTGGCG CTCCACCAGC CCTGATGGTG GCCCCCGCTC TGCTGGCTGG
    CGGGAACATG GGGACCGGCG TCGCAAGTTT GAATTTGATT TGCGAGGGGA TCGAGGAGGG
    TGTGGTGAAG AGGAGGGGCG GGGTGGGGGG GGGAGCTCTC ACCTCCGGAG ATGCAGGGGG
    CCTGACGGCT TCGATGACGA CAAGGATGGG CTCCCGGAGT GGTGCCTGGA TGATGAGGAC
    GAAGAAATGG GCACCTTTGA TGCCTCCGGG GCCTTCTTGC CTCTCAAGAA GGGCCCCAAG
    GAGCCCATTC CTGAGGAGCA GGAGCTCGAC TTCCAGGGCC TGGAGGAAGA GGAGGAAGAA
    CCTTCCGAAG GGCTAGACGA GGAGGGGCCC GAGGCGGGTG GGAAGGAACT GACCCCGCTG
    CCTCCTCAGG AGGAGAAGTC CAGCTCCCCG TCCCCACTGC CCACCTTGGG CCCGCTCTGG
    GGAGCTAACG GGGAAGGCGA TGACCCAGTG GAGAAAGACC TGCCTGCAGC TGAAGGAGAG
    GACATGAGGG GAATGCAGCT AAGTCCTGGG GCGGGCTCAC CGCCTGGCCC ACCAGACCTG
    GAAGATGATG AAGGCTTGAA GCACCTGCAG CAGGAGGCGG AGAAGCTCGT GGCCTCCCTG
    CAGGACAGCT CCCTGGAGGA GGAGCAGTTC ACGGCCGCCA TCCAGGCCCA GGGCCTGCGT
    CACTCTGCAG CCGCCACTGC CCTCCCCCTC AGCCATGGTG CGGCCCGGAA GTGGTTCTAC
    AAGGACCCGC AGGGCGAGAT CCAAGGCCCC TTCACGACCC AGGAGATGGC GGAGTGGTTC
    CAGGCGGGCT ACTTCTCCAT GGCCCTGCTG GTGAAGCGGG GCTGTGATGA GGGCTTCCAG
    CCACTGGGTG AGGTGATCAA GATGTGGGGT CGCGTACCCT TTGCCCCTGG GCCCTCACCT
    CCCCCGCTGC TGGTGAGTGT ACCCCCTTTG GCGTGGAACA GGGAAGGGAG GCAGGAGCCT
    GGCAGAGGGC AGCGGTGGGT GCAGAGCCAG ACAGGGGCAA AGCCACGGGA ACGCTGTGGC
    CTCACTCAGG GAAACATGGA CCAGGAGCGG CTGAAGAAGC AACAGGAGCT GGCAGCAGCC
    GCCTTGTACC AGCAGCTGCA GCACCAGCAG TTTCTTCAGC TGGTCGGCAG CCGCCAGCTC
    CCGCAGTGCG TGCTCCGGGA AAAGGCAGCT CTGGGAGACC TGCCGCCGCC GCAGCAGCAG
    CAGCTCACCG CGTTCCTGCA GCAGCTCCAA GCTCTCAAAC CCCCCAGGGG CGGGGACCAG
    AACTTGCTCC CGACGATGAA CCGGTCCTTG TCGGTGCCAG ACTCGGGTCC CCTCTGGGAC
    ATACATACCT CAGCCTCACC ACAGTCAGGC GGTGAGGCCA GTCTTTGGGA TATACCAATT
    AACTCTTCGA CTCAGGGTCC AATTCTAGAA CAACTCCAGC TGCAACACAA ATTCCAGGAG
    CGCAGAGAAG TGGAGCTCAG GGCGAAGCGG GAGGAGGAGG AGCGGAAGCG CCGGGAAGAG
    AAGCGCCGCC AGCAGCAGCA GCAGCAGGAG GAGCAGAAGC GGCGGCAGGA GGAAGAGGAG
    CTGTTCCGGC GCAAGCAGGC GCGGCAGCAG GAGCTGCTGC TGAAGCTGCT GCAGCAGCAG
    CAGCAGGCGG TGGCCGCCGT CCCCGCGCCT CCTACACCCA GTTCCCCGCC TCCGCTGTGG
    GCTGGCCTGG CCAAGCAGGG CCTGTCCATG AAGACGCTGC TGGAGCTGCA GCTGGAGGGC
    GAGCGGCACC TGCATAAGCA GCCCCCGCCT CGGGAGCCAT CGCGGGCCCA GGCCCCCAAC
    CACCGCGTGC AGCTCGGGGG CCTGGGCTCC GCCCCCCTGA ACCAGTGGGT ATCTGAGGCC
    GGGCCGCTGT GGGGTGGGCC CGACAAGAGT GGGGGCGGCA GCAGCAGCCT GGGCCTCTGG
    GAAGACACCC TCAAGAGCAG TGGGAGCCTG GCCCGGAGCC TGGGCCTGAA GAACAGCCGC
    AGCAGCCCCT CTCTCAGTGA CTCGTACAGC CACTTGTCTG GTCGGCCTGT GCGCAAAAAG
    ACGGAGGAGG AAGAGAAGCT GCTGAAGCTG CTGCAGGGCA TCCCCCGGCC CCAGGATGGC
    TTCACCCAGT GGTGTGAGCA GATGCTGCAC ACGCTGAGCA CCACGGGCAG CCTGGACGTG
    CCCATGGCTG TAGCGATCCT CAAGGAGGTG GAATCCCCCT ATGACGTCCA CGACTATATT
    CGTTCCTGCC TGGGGGACAC GCTGGAAGCC AAAGAATTTG CCAAACAATT CCTGGAGCGG
    AGGGCCAAGC AGAAGGCCAG CCAGCAGCGG CAGCAGCAGC AGGAGGCTTG GCTGAGCAGC
    GGCTCCCTAC AGACAGCCTT TCAGACCAAC CACAGCACCA AGCTTGGCCC CGGGGAGGGC
    AGCAAGGCCA AGAGGCGGGC ACTGATGCTG CACTCGGACC CCAGCATCTT GGGGTACTCC
    CTGCACGGAC CTTCTGGTGA GATCGAGAGC GTGGATGACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.