TLR8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR8
Entrez Gene ID	532262
Full Name	toll like receptor 8
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005228488.3	XP_005228545.1	toll-like receptor 8 isoform X1
XM_024988093.1	XP_024843861.1	toll-like receptor 8 isoform X1
XM_005228489.3	XP_005228546.1	toll-like receptor 8 isoform X1
XM_005228490.4	XP_005228547.1	toll-like receptor 8 isoform X1
XM_005228489.4	XP_005228546.1	toll-like receptor 8 isoform X1
NM_001033937.1	NP_001029109.1	toll-like receptor 8 precursor
XM_005228490.3	XP_005228547.1	toll-like receptor 8 isoform X1
XM_005228488.4	XP_005228545.1	toll-like receptor 8 isoform X1

Pathways from BioSystems

Homologies

	TLR8	NP_001123899.1
Mus musculus (house mouse)	Tlr8	NP_573475.2
Xenopus tropicalis (tropical clawed frog)	LOC100495962	XP_004912402.1
Homo sapiens (human)	TLR8	NP_619542.1
Rattus norvegicus (Norway rat)	Tlr8	NP_001094479.1
Bos taurus (cattle)	TLR8	NP_001029109.1
Danio rerio (zebrafish)	LOC100332583	XP_002665954.3
Xenopus tropicalis (tropical clawed frog)	tlr8	XP_002933859.1
Pan troglodytes (chimpanzee)	TLR8	NP_001123944.1
Canis lupus familiaris (dog)	TLR8	XP_005641176.1
Danio rerio (zebrafish)	tlr8a	XP_001920594.3

Gene Ontology
Bibliography

Related articles in PubMed

Toll-like receptors 3, 7 and 8 are upregulated in the placental caruncle and fetal spleen of Neospora caninum experimentally infected cattle.
Marin MS, Hecker YP, Quintana S, P?rez SE, Leunda MR, Cant?n GJ, Cobo ER, Moore DP, Ode?n AC
Veterinary parasitology23658-61(2017 Mar)

Involvement of toll-like receptors 3 and 7/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5.
Rensetti D, Marin M, Quintana S, Mor?n P, Verna A, Ode?n A, P?rez S
Research in veterinary science1071-7(2016 Aug)

Inhibition of TLR8 mediated signaling promotes BCG induced apoptosis in THP-1 cells.
Tang J, Zhan L, Qin C
Microbial pathogenesis9378-82(2016 Apr)

A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition.
Liu J, Xu C, Hsu LC, Luo Y, Xiang R, Chuang TH
Molecular immunology47(5)1083-90(2010 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

On day 104 of pregnancy, mRNA expression of TLRs 3 and 8, as well as that of TLRs 7 and 9, was high in the spleen of fetuses from Neospora caninum-infected heifers. Gene expression levels of endosomal TLRs were also detectable in the placenta and the maternal caruncle from infected heifers, being TLRs 3, 7 and 8 particularly upregulated, mostly in the caruncle.
Title: Toll-like receptors 3, 7 and 8 are upregulated in the placental caruncle and fetal spleen of Neospora caninum experimentally infected cattle.

The results from this study demonstrate that expression of at least TLR3, TLR7 and TLR8 is stimulated upon bovine alpha-herpesvirus infection of the brain.
Title: Involvement of toll-like receptors 3 and 7/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5.

Knockdown TLR8 increased the apoptosis induced by Bacillus Calmette Guerin infection, and this enhanced apoptosis was caspase-dependent.
Title: Inhibition of TLR8 mediated signaling promotes BCG induced apoptosis in THP-1 cells.

A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition.
Title: A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition.

multiple regions, including ECD, TM, linker and TIR-tail regions of bTLR8, are involved in determining the localization of cellular ER compartment.
Title: Multiple molecular regions confer intracellular localization of bovine Toll-like receptor 8.

The following TLR8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa02230	NM_001033937.1 Latest version!	Bos taurus toll like receptor 8 (TLR8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35840	XM_005228490.4 Latest version!	Bos taurus toll like receptor 8 (TLR8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35840	XM_005228488.4 Latest version!	Bos taurus toll like receptor 8 (TLR8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35840	XM_024988093.1 Latest version!	Bos taurus toll like receptor 8 (TLR8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35840	XM_005228489.4 Latest version!	Bos taurus toll like receptor 8 (TLR8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa35840	XM_005228489.3	Bos taurus toll like receptor 8 (TLR8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35840	XM_005228490.3	Bos taurus toll like receptor 8 (TLR8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35840	XM_005228488.3	Bos taurus toll like receptor 8 (TLR8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR8 ( NM_001033937.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029109.1 Bos taurus toll like receptor 8 (TLR8), mRNA.

Price & Availability↑

CloneID	OBa02230
Clone ID Related Accession (Same CDS sequence)	NM_001033937.1
Accession Version	NM_001033937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-10
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY642125.2. On Oct 4, 2005 this sequence version replaced XM_610774.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY642125.2 [ECO:0000332] ##Evidence-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATG 
CCCTCCTTAG AAATACTTGA TTTATCTTAC AACTATGAAC TGAAAAAATA CCCTCAGTAC 
ATTAACATTT CCAAAAATTT CTCGAAGCTT ATATCTCTCC AGATGTTGCA TTTAAGAGGT 
TATGTGTTCC AGGAACTTAG AAAGGAAGAT TTCGAGCCCC TGCGGAACCT CTCAAATTTA 
ACGACTATCA ACTTGGGCGT TAACTTTATT AAGCAGATTG ATTTTAGTAT TTTCCACTGG 
TTCCCCAACC TGAAAATCAT TTACTTGTCA GAAAACAGAA TATCACCCTT GGTCAGTGAT 
ACCGAGCAAC ATGATGCAAA TGGGACCTCT TTCCAAAGTC ACATCCTGAA GCGACGCTCA 
GCCGATATTC AATTTGACCC ACATTCGAAT TTTTATCATA ACACCCGTCC TTTAATAAAG 
ACAGAATGTT CACGTCTTGG CAGTGCCTTA GATTTAAGCT TGAACAGTAT TTTCTTTATT 
GGGGTAAGCC AGTTTAAAGA TTTTGGCAAC ATTTCCTGTT TAAATCTATC TTCAAATGGC 
AATGGCCAGG TGTTAAATGG AACGGAATTT TCATGCTTGT CTGGTATCAA GTATTTGGAT 
TTGACAAACA ATAGACTAGA CTTTGATGAC GATGCTGCTT TCAGTGAATT GCCATTGTTA 
GAAGTTCTCG ATCTCAGCTA CAATGCGCAC TATTTCCGAA TAGCAGGGGT AACGCACCGT 
CTAGGATTTA TTGAACATTT AACTAACCTG AGAGTTTTAA ACTTGAGCAA CAATGACATT 
TTTACTTTAA CAGAAACACA ACTGAAAAGC GCGTCCCTGG GAGAATTAGT GTTCAGTGGG 
AACCGCCTTG ACCTTCTGTG GAATGCTCAA GATGTCAGGT ACTGGCAAAT TTTTCAAAAT 
CTCAAAGATC TGACCCGGCT TGACTTAGCC CGTAATAACC TTCGGAATGT CTCCAGTCAG 
GCCTTCCTTA ACTTGCCCCA GACTCTCACC GACCTACATA TAAATGATAA TATGTTAAAG 
TTTTTTAACT GGACATTACT CCAGCAGTTT CCTCGTCTCG AGTTGCTTGA CTTAAGTGGA 
AACCAGCTGT TCTTTTTAAC CAATAGCCTA TCTACATTTG CATCTTCTCT TGAGACGCTA 
CTGCTGAGTC GAAACAGAAT TTCCCACCTG CCGTCTGATT TTCTTTCTGG AGCCAGCAGC 
CTGATACACC TCGATTTGAA CTCCAACCAG CTCAAGATGC TCAACAGATC CACATTTGAA 
ACGAAGACCG CCACCAAGTT AACTGTTTTG GAACTAGGGG GTAACCCTTT TGACTGTACC 
TGTGACTTCG GAGATTTTCT AGAATGGATG GACAGAAATC TGAACGTCAG GGTTCCCAGA 
CTGACCGATG TCATTTGTGC CAGTCCTGGG GATCAAGAAG GCAAGAGCAT TGTGAGTCTA 
GACCTCAGCA CTTGTGTTTC AGATACCATT GCAGCCATAT TCTGTTTCTT AACCTTTTCT 
GTCACCATCT CAGTTATGTT GGCTGCCCTG GCTCACCACT GGTTTTACTG GGATGCTTGG 
TTCATCTACC ATGTGTGCTT AGCCAAGGTA AAAGGCTACA GGTCTCTTTC CACATCCCAG 
ACTTTCTATG ATGCTTACAT TTCTTATGAC ACCAAAGACG CCTCTGTCAC AGACTGGGTG 
ATGAATGAGC TGCGCTTCCA CCTGGAAGAG AGTGAGGACA AGAATGTGCT CCTGTGTTTA 
GAGGAAAGGG ATTGGGACCC GGGTCTAGCC ATCATCGACA ACCTCATGCA GAGCATCAAC 
CAAAGCAAGA AAACAATATT TGTTTTAACC AAAAAATATG CCAAAAACTG GAATTTTAAA 
ACGGCATTCT ACTTGGCCTT GCAGAGGCTA ATGGAGGAGA ATATGGACGT GATTGTCTTT 
ATTCTGCTGG AGCCAGTGTT GCAGCATTCG CAGTATTTGA GACTGCGGCA GAGGATCTGC 
AAGAGTTCCA TCCTCCAGTG GCCTGATAAC CCCAAGGCGG AGGGCTTGTT TTGGCAAAGT 
CTGAAAAATG TCGTCCTAAC AGCCAACGAT TCACGGTATA ACAACTTGTA TGTCAATTCC 
ATTAAGCAAT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029109.1
CDS	1..3075
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEMPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLKDLTRLDLARNNLRNVSSQAFLNLPQTLTDLHINDNMLKFFNWTLLQQFPRLELLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVMNELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	NM_001033937.1
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033937.1

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATG 
CCCTCCTTAG AAATACTTGA TTTATCTTAC AACTATGAAC TGAAAAAATA CCCTCAGTAC 
ATTAACATTT CCAAAAATTT CTCGAAGCTT ATATCTCTCC AGATGTTGCA TTTAAGAGGT 
TATGTGTTCC AGGAACTTAG AAAGGAAGAT TTCGAGCCCC TGCGGAACCT CTCAAATTTA 
ACGACTATCA ACTTGGGCGT TAACTTTATT AAGCAGATTG ATTTTAGTAT TTTCCACTGG 
TTCCCCAACC TGAAAATCAT TTACTTGTCA GAAAACAGAA TATCACCCTT GGTCAGTGAT 
ACCGAGCAAC ATGATGCAAA TGGGACCTCT TTCCAAAGTC ACATCCTGAA GCGACGCTCA 
GCCGATATTC AATTTGACCC ACATTCGAAT TTTTATCATA ACACCCGTCC TTTAATAAAG 
ACAGAATGTT CACGTCTTGG CAGTGCCTTA GATTTAAGCT TGAACAGTAT TTTCTTTATT 
GGGGTAAGCC AGTTTAAAGA TTTTGGCAAC ATTTCCTGTT TAAATCTATC TTCAAATGGC 
AATGGCCAGG TGTTAAATGG AACGGAATTT TCATGCTTGT CTGGTATCAA GTATTTGGAT 
TTGACAAACA ATAGACTAGA CTTTGATGAC GATGCTGCTT TCAGTGAATT GCCATTGTTA 
GAAGTTCTCG ATCTCAGCTA CAATGCGCAC TATTTCCGAA TAGCAGGGGT AACGCACCGT 
CTAGGATTTA TTGAACATTT AACTAACCTG AGAGTTTTAA ACTTGAGCAA CAATGACATT 
TTTACTTTAA CAGAAACACA ACTGAAAAGC GCGTCCCTGG GAGAATTAGT GTTCAGTGGG 
AACCGCCTTG ACCTTCTGTG GAATGCTCAA GATGTCAGGT ACTGGCAAAT TTTTCAAAAT 
CTCAAAGATC TGACCCGGCT TGACTTAGCC CGTAATAACC TTCGGAATGT CTCCAGTCAG 
GCCTTCCTTA ACTTGCCCCA GACTCTCACC GACCTACATA TAAATGATAA TATGTTAAAG 
TTTTTTAACT GGACATTACT CCAGCAGTTT CCTCGTCTCG AGTTGCTTGA CTTAAGTGGA 
AACCAGCTGT TCTTTTTAAC CAATAGCCTA TCTACATTTG CATCTTCTCT TGAGACGCTA 
CTGCTGAGTC GAAACAGAAT TTCCCACCTG CCGTCTGATT TTCTTTCTGG AGCCAGCAGC 
CTGATACACC TCGATTTGAA CTCCAACCAG CTCAAGATGC TCAACAGATC CACATTTGAA 
ACGAAGACCG CCACCAAGTT AACTGTTTTG GAACTAGGGG GTAACCCTTT TGACTGTACC 
TGTGACTTCG GAGATTTTCT AGAATGGATG GACAGAAATC TGAACGTCAG GGTTCCCAGA 
CTGACCGATG TCATTTGTGC CAGTCCTGGG GATCAAGAAG GCAAGAGCAT TGTGAGTCTA 
GACCTCAGCA CTTGTGTTTC AGATACCATT GCAGCCATAT TCTGTTTCTT AACCTTTTCT 
GTCACCATCT CAGTTATGTT GGCTGCCCTG GCTCACCACT GGTTTTACTG GGATGCTTGG 
TTCATCTACC ATGTGTGCTT AGCCAAGGTA AAAGGCTACA GGTCTCTTTC CACATCCCAG 
ACTTTCTATG ATGCTTACAT TTCTTATGAC ACCAAAGACG CCTCTGTCAC AGACTGGGTG 
ATGAATGAGC TGCGCTTCCA CCTGGAAGAG AGTGAGGACA AGAATGTGCT CCTGTGTTTA 
GAGGAAAGGG ATTGGGACCC GGGTCTAGCC ATCATCGACA ACCTCATGCA GAGCATCAAC 
CAAAGCAAGA AAACAATATT TGTTTTAACC AAAAAATATG CCAAAAACTG GAATTTTAAA 
ACGGCATTCT ACTTGGCCTT GCAGAGGCTA ATGGAGGAGA ATATGGACGT GATTGTCTTT 
ATTCTGCTGG AGCCAGTGTT GCAGCATTCG CAGTATTTGA GACTGCGGCA GAGGATCTGC 
AAGAGTTCCA TCCTCCAGTG GCCTGATAAC CCCAAGGCGG AGGGCTTGTT TTGGCAAAGT 
CTGAAAAATG TCGTCCTAAC AGCCAACGAT TCACGGTATA ACAACTTGTA TGTCAATTCC 
ATTAAGCAAT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR8 ( XM_005228488.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228545.1 Bos taurus toll like receptor 8 (TLR8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35840
Clone ID Related Accession (Same CDS sequence)	XM_005228488.3 , XM_024988093.1 , XM_005228489.3 , XM_005228490.4 , XM_005228489.4 , XM_005228490.3 , XM_005228488.4
Accession Version	XM_005228488.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104782.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228545.1
CDS	196..3297
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEIASGEFLTKLPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLTNLTRLDLARNNLRHISSQAFLNLPRTLTDLYINDNMLNFFNWSLLEYFPHLRLLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	XM_005228488.3
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228488.3

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR8 ( XM_024988093.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843861.1 Bos taurus toll like receptor 8 (TLR8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa35840
Clone ID Related Accession (Same CDS sequence)	XM_005228488.3 , XM_024988093.1 , XM_005228489.3 , XM_005228490.4 , XM_005228489.4 , XM_005228490.3 , XM_005228488.4
Accession Version	XM_024988093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843861.1
CDS	152..3253
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEIASGEFLTKLPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLTNLTRLDLARNNLRHISSQAFLNLPRTLTDLYINDNMLNFFNWSLLEYFPHLRLLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	XM_024988093.1
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988093.1

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR8 ( XM_005228489.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228546.1 Bos taurus toll like receptor 8 (TLR8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35840
Clone ID Related Accession (Same CDS sequence)	XM_005228488.3 , XM_024988093.1 , XM_005228489.3 , XM_005228490.4 , XM_005228489.4 , XM_005228490.3 , XM_005228488.4
Accession Version	XM_005228489.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104782.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228489.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228546.1
CDS	199..3300
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEIASGEFLTKLPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLTNLTRLDLARNNLRHISSQAFLNLPRTLTDLYINDNMLNFFNWSLLEYFPHLRLLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	XM_005228489.3
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228489.3

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR8 ( XM_005228490.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228547.1 Bos taurus toll like receptor 8 (TLR8), transcript variant X2, mRNA.

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CloneID	OBa35840
Clone ID Related Accession (Same CDS sequence)	XM_005228488.3 , XM_024988093.1 , XM_005228489.3 , XM_005228490.4 , XM_005228489.4 , XM_005228490.3 , XM_005228488.4
Accession Version	XM_005228490.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228490.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228547.1
CDS	160..3261
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEIASGEFLTKLPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLTNLTRLDLARNNLRHISSQAFLNLPRTLTDLYINDNMLNFFNWSLLEYFPHLRLLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	XM_005228490.4
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228490.4

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR8 ( XM_005228489.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228546.1 Bos taurus toll like receptor 8 (TLR8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35840
Clone ID Related Accession (Same CDS sequence)	XM_005228488.3 , XM_024988093.1 , XM_005228489.3 , XM_005228490.4 , XM_005228489.4 , XM_005228490.3 , XM_005228488.4
Accession Version	XM_005228489.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228489.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228546.1
CDS	216..3317
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEIASGEFLTKLPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLTNLTRLDLARNNLRHISSQAFLNLPRTLTDLYINDNMLNFFNWSLLEYFPHLRLLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	XM_005228489.4
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228489.4

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR8 ( XM_005228490.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228547.1 Bos taurus toll like receptor 8 (TLR8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35840
Clone ID Related Accession (Same CDS sequence)	XM_005228488.3 , XM_024988093.1 , XM_005228489.3 , XM_005228490.4 , XM_005228489.4 , XM_005228490.3 , XM_005228488.4
Accession Version	XM_005228490.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104782.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228547.1
CDS	132..3233
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEIASGEFLTKLPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLTNLTRLDLARNNLRHISSQAFLNLPRTLTDLYINDNMLNFFNWSLLEYFPHLRLLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	XM_005228490.3
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228490.3

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR8 ( XM_005228488.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228545.1 Bos taurus toll like receptor 8 (TLR8), transcript variant X1, mRNA.

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CloneID	OBa35840
Clone ID Related Accession (Same CDS sequence)	XM_005228488.3 , XM_024988093.1 , XM_005228489.3 , XM_005228490.4 , XM_005228489.4 , XM_005228490.3 , XM_005228488.4
Accession Version	XM_005228488.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	toll-like receptor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228488.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228545.1
CDS	212..3313
Translation	MTLHFLLLTSLFLLISDSCEFFTEASYPRSYPCDVKNENGSFIAECNGRRLQEVPQTVDKDVTEVDLSDNFITRVTNESFQGLQNLTKINLNHNAKSQSGNPAVKKAMTITDGAFLNLKHLRELLLEDNQLQQIPAGLPESLKKLSLIQNNIITLTKKNTSGLGNLESLYLGWNCYFACDKKFTIENGAFQNLTKLKVLSLSFNPLHSVPPSLPSSLTELYLSNTHIGNVSEEDFKELSNLRVLDLSGNCPRCFNAPFPCVPCQGDASIQIHPLAFQTLTQLRYLNLSSTSLRKVPASWFDNMHNLKVLDLEFNYLMDEIASGEFLTKLPSLEILDLSYNYELKKYPQYINISKNFSKLISLQMLHLRGYVFQELRKEDFEPLRNLSNLTTINLGVNFIKQIDFSIFHWFPNLKIIYLSENRISPLVSDTEQHDANGTSFQSHILKRRSADIQFDPHSNFYHNTRPLIKTECSRLGSALDLSLNSIFFIGVSQFKDFGNISCLNLSSNGNGQVLNGTEFSCLSGIKYLDLTNNRLDFDDDAAFSELPLLEVLDLSYNAHYFRIAGVTHRLGFIEHLTNLRVLNLSNNDIFTLTETQLKSASLGELVFSGNRLDLLWNAQDVRYWQIFQNLTNLTRLDLARNNLRHISSQAFLNLPRTLTDLYINDNMLNFFNWSLLEYFPHLRLLDLSGNQLFFLTNSLSTFASSLETLLLSRNRISHLPSDFLSGASSLIHLDLNSNQLKMLNRSTFETKTATKLTVLELGGNPFDCTCDFGDFLEWMDRNLNVRVPRLTDVICASPGDQEGKSIVSLDLSTCVSDTIAAIFCFLTFSVTISVMLAALAHHWFYWDAWFIYHVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRFHLEESEDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTIFVLTKKYAKNWNFKTAFYLALQRLMEENMDVIVFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQSLKNVVLTANDSRYNNLYVNSIKQY

Target ORF information:

RefSeq Version	XM_005228488.4
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 8 (TLR8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228488.4

ORF Insert Sequence:

ATGACCCTTC ACTTTTTGCT TCTGACATCC CTTTTCCTGC TCATCTCTGA TTCCTGTGAG 
TTCTTCACTG AAGCCAGTTA TCCCCGAAGC TATCCTTGCG ATGTGAAAAA CGAAAATGGC 
TCTTTTATTG CAGAATGTAA TGGTCGTCGA TTACAGGAAG TACCCCAAAC AGTGGACAAA 
GATGTGACGG AAGTAGACCT GTCTGATAAT TTCATCACAC GGGTAACGAA TGAATCCTTT 
CAAGGGCTGC AAAATCTGAC TAAAATCAAC CTGAACCATA ATGCCAAGTC CCAGAGTGGA 
AATCCTGCTG TAAAGAAAGC TATGACTATT ACAGACGGGG CATTTCTCAA CCTCAAACAC 
CTAAGGGAGT TGCTGCTGGA AGACAACCAG TTACAACAAA TACCAGCTGG TTTGCCAGAA 
TCTTTAAAAA AACTTAGTCT AATTCAAAAC AACATAATTA CGTTAACGAA AAAGAACACT 
TCTGGACTTG GGAACCTGGA AAGTCTCTAT TTGGGCTGGA ACTGTTATTT TGCTTGTGAT 
AAAAAATTTA CCATAGAAAA TGGAGCATTC CAAAACCTTA CCAAGTTGAA GGTGCTGTCA 
TTATCTTTTA ATCCCCTTCA CAGCGTGCCA CCAAGTCTGC CAAGCTCGCT AACAGAACTC 
TACCTTAGTA ATACCCATAT TGGAAACGTC AGTGAAGAAG ACTTCAAGGA ACTGAGCAAT 
TTAAGAGTAC TAGATTTAAG TGGAAACTGC CCGAGATGTT TTAACGCTCC ATTTCCCTGT 
GTACCTTGCC AAGGAGATGC TTCAATTCAG ATACACCCTC TTGCTTTTCA AACCCTGACC 
CAACTTCGCT ACCTAAACCT CTCTAGCACT TCGCTCAGGA AGGTTCCTGC CAGCTGGTTT 
GACAACATGC ACAATCTGAA GGTATTGGAT CTTGAATTCA ACTATTTAAT GGACGAAATA 
GCCTCGGGGG AATTTTTGAC AAAATTGCCC TCCTTAGAAA TACTTGATTT ATCTTACAAC 
TATGAACTGA AAAAATACCC TCAGTACATT AACATTTCCA AAAATTTCTC GAAGCTTATA 
TCTCTCCAGA TGTTGCATTT AAGAGGTTAT GTGTTCCAGG AACTTAGAAA GGAAGATTTC 
GAGCCCCTGC GGAACCTCTC AAATTTAACG ACTATCAACT TGGGCGTTAA CTTTATTAAG 
CAGATTGATT TTAGTATTTT CCACTGGTTC CCCAACCTGA AAATCATTTA CTTGTCAGAA 
AACAGAATAT CACCCTTGGT CAGTGATACC GAGCAACATG ATGCAAATGG GACCTCTTTC 
CAAAGTCACA TCCTGAAGCG ACGCTCAGCC GATATTCAAT TTGACCCACA TTCGAATTTT 
TATCATAACA CCCGTCCTTT AATAAAGACA GAATGTTCAC GTCTTGGCAG TGCCTTAGAT 
TTAAGCTTGA ACAGTATTTT CTTTATTGGG GTAAGCCAGT TTAAAGATTT TGGCAACATT 
TCCTGTTTAA ATCTATCTTC AAATGGCAAT GGCCAGGTGT TAAATGGAAC GGAATTTTCA 
TGCTTGTCTG GTATCAAGTA TTTGGATTTG ACAAACAATA GACTAGACTT TGATGACGAT 
GCTGCTTTCA GTGAATTGCC ATTGTTAGAA GTTCTCGATC TCAGCTACAA TGCGCACTAT 
TTCCGAATAG CAGGGGTAAC GCACCGTCTA GGATTTATTG AACATTTAAC TAACCTGAGA 
GTTTTAAACT TGAGCAACAA TGACATTTTT ACTTTAACAG AAACACAACT GAAAAGCGCG 
TCCCTGGGAG AATTAGTTTT CAGTGGGAAC CGCCTTGACC TTCTGTGGAA TGCTCAAGAT 
GTCAGGTACT GGCAAATTTT TCAAAATCTC ACCAATCTGA CCCGGCTTGA CTTAGCCCGT 
AATAACCTTC GGCATATCTC CAGTCAGGCC TTCCTTAACT TGCCCAGGAC TCTCACTGAC 
CTATATATAA ATGATAACAT GTTAAATTTC TTTAACTGGT CATTACTGGA ATACTTCCCT 
CACCTCAGAT TGCTTGACTT AAGTGGAAAC CAGCTGTTCT TTTTAACCAA TAGCCTATCT 
ACATTTGCAT CTTCTCTTGA GACGCTACTG CTGAGTCGAA ACAGAATTTC CCACCTGCCG 
TCTGATTTTC TTTCTGGAGC CAGCAGCCTG ATACACCTCG ATTTGAACTC CAACCAGCTC 
AAGATGCTCA ACAGATCCAC ATTTGAAACG AAGACCGCCA CCAAGTTAAC TGTTTTGGAA 
CTAGGGGGTA ACCCTTTTGA CTGTACCTGT GACTTCGGAG ATTTTCTAGA ATGGATGGAC 
AGAAATCTGA ACGTCAGGGT TCCCAGACTG ACCGATGTCA TTTGTGCCAG TCCTGGGGAT 
CAAGAAGGCA AGAGCATTGT GAGTCTAGAC CTCAGCACTT GTGTTTCAGA TACCATTGCA 
GCCATATTCT GTTTCTTAAC CTTTTCTGTC ACCATCTCAG TGATGCTGGC TGCCCTGGCC 
CACCACTGGT TTTACTGGGA TGCTTGGTTT ATCTACCATG TGTGCTTAGC TAAGGTCAAA 
GGCTACAGGT CTCTGTCCAC ATCCCAGACT TTCTATGATG CTTACATTTC TTATGACACC 
AAAGACGCTT CTGTCACGGA CTGGGTGATC AATGAATTGC GCTTCCACCT GGAAGAGAGT 
GAGGACAAGA ATGTGCTCCT GTGTTTAGAG GAAAGGGATT GGGACCCGGG TCTAGCCATC 
ATCGACAACC TCATGCAGAG CATCAACCAA AGCAAGAAAA CAATATTTGT TTTAACCAAA 
AAATATGCCA AAAACTGGAA TTTTAAAACG GCATTCTACT TGGCCTTGCA GAGGCTAATG 
GAGGAGAATA TGGACGTGAT TGTCTTTATT CTGCTGGAGC CAGTGTTGCA GCATTCGCAG 
TATTTGAGAC TGCGGCAGAG GATCTGCAAG AGTTCCATCC TCCAGTGGCC TGATAACCCC 
AAGGCGGAAG GCTTGTTTTG GCAGAGTCTG AAAAATGTCG TCCTAACAGC CAACGATTCA 
CGGTATAACA ACTTGTATGT CAATTCCATT AAGCAATACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Toll-like receptors 3, 7 and 8 are upregulated in the placental caruncle and fetal spleen of Neospora caninum experimentally infected cattle.
Veterinary parasitology23658-61(2017 Mar)
Marin MS,Hecker YP,Quintana S,P?rez SE,Leunda MR,Cant?n GJ,Cobo ER,Moore DP,Ode?n AC

Involvement of toll-like receptors 3 and 7/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5.
Research in veterinary science1071-7(2016 Aug)
Rensetti D,Marin M,Quintana S,Mor?n P,Verna A,Ode?n A,P?rez S

Inhibition of TLR8 mediated signaling promotes BCG induced apoptosis in THP-1 cells.
Microbial pathogenesis9378-82(2016 Apr)
Tang J,Zhan L,Qin C

A five-amino-acid motif in the undefined region of the TLR8 ectodomain is required for species-specific ligand recognition.
Molecular immunology47(5)1083-90(2010 Feb)
Liu J,Xu C,Hsu LC,Luo Y,Xiang R,Chuang TH

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TLR8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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