MCF2 cDNA ORF clone, Bos taurus(cattle)

The following MCF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa89236 XM_015470209.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa89236 XM_015461454.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa89237 XM_015470210.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa89237 XM_015461455.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa238713 XM_024988613.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa89233 XM_015461451.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89233 XM_015470206.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89234 XM_015461452.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89234 XM_015470207.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89235 XM_015461453.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa89235 XM_015470208.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa12869 XM_005200582.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa12869 XM_005227557.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa12870 XM_005200583.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa12870 XM_005227558.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa89236
    Clone ID Related Accession (Same CDS sequence) XM_015470209.1 , XM_015461454.1
    Accession Version XM_015470209.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325695.1
    CDS317..3304
    Translation

    Target ORF information:

    RefSeq Version XM_015470209.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470209.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89236
    Clone ID Related Accession (Same CDS sequence) XM_015470209.1 , XM_015461454.1
    Accession Version XM_015461454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316940.1
    CDS317..3304
    Translation

    Target ORF information:

    RefSeq Version XM_015461454.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89237
    Clone ID Related Accession (Same CDS sequence) XM_015470210.1 , XM_015461455.1
    Accession Version XM_015470210.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGCTATAGAA
    AATTTTGCCC TCACAGTGAA AGATACGGCT CAGATGTTAC AGTCCTTTGG AACTGAACTG
    GCTGAGACAG AACTACCAGG TGACATTCCT GCAATGGAAG AAATTCTTGC AATTCGTGCT
    GAAAGGTACC ACCTGTTAAA GAAGGATATC ACAGCTGTAA CCAAAGAAGG AAAATTTCTG
    CTAACAAATC TGGAAGTGCC TGATGCTGAG GGTGCTGTGA GTTCAAGACT TGAAAATGAT
    CAGCCGACTA GTGGTGACTG GCAAACTATT AATAAATTGC TGACTCAAGT ACATGATATG
    GAAATAGCTT TTGATGGATT TTGGGAAAAA CACCAGCTAA AAATGGAGCA GTATTTGCAA
    CTCTGGAAGT TTGAGCAGGA TTTTCAAGAG CTCGTGAGTG AAGCTGAACT TATACTAAAT
    CAACAAGCGG AGTTGGCAGA TGTAACAGGG ACTATAGCTC AAGTGAAACA AAAAATTAAA
    AAGTTGGAAA ACTTGGATGA AAATTCTCAG GACCTGTTAG CAAAGGCCCG GTTCGTAATA
    ATACATGGAC ATAGGCTCGC AGCAAGTCAT CATTATGCCC TGGATTTGAT CTGTCAGAGG
    TGCAATGAAC TACGTTACCT TTCTGATATT TTGGTAAATG AAATCAGAAC CAAACGGATA
    CAGCTCAACA GGACCTTTAA AATGCATAAA CTCCTACAGC AGGCTCTCCA GTGCTGTGAT
    GAAGGAGAGT GCCTTCTGGC TAATCAAGAA ATAGACAAGT TTCAGTCTAA AGAAGATGCA
    CAGAAGGCCC TCCAAGACAT TGAAAATTTT CTTGAAATGG CTCTACCCTT TATAAATTAT
    GATCCTGAAA CCCTACAGTA TGAATTTGGT ATAATTTTAT CTCCTGAACT CAAGGTTCAA
    ATGCAGACCA TCCAACTCAA GCTTGAAAAC ATCCGAAGTA TATTTGAGAA CCAACAGGCT
    GGTTTCAGAA ACCTGACAGA TAAACATGTG AGGCCGATTC CGTTTGTAGT ATCTGCTTCT
    GACAATTTGG TCAGATCTGG AGCACCATTT TTTTCACCTA CACAAGGGAA GAAGACTTGG
    AGACAAAATC AGAGCAACAC AAAAACTGAA GCAGTGTATG ATTGTCAGGA GAAGAGAAGT
    TCTGCTCAGT CCTCCAATTT GGACAATGAC AATAGCTTGG ATATTTTAAA GAACCACGTC
    CTAAATGAGC TGATACAGAC GGAGAGGGCA TATGTTAGGG AACTGTTTAC TGTTTTGTTG
    GGCTATAGAG CGGAGATGGA TAATCCAGAG ATGTTTGTTC TTATGCCACC TCTCCTGAGA
    AGTAAAAGGG ATGTTCTCTT TGGAAACATG GCAGAAATAT ATGAATTCCA TAACAACATT
    TTCATGAGCA GTCTGGAAAA TTGTGTTGAT GCTCCAGAAA GAGTGGGATC TTGTTTCCTG
    GAAAGGAAAG ATGATTTTCA AATGTATGCG AAGTATTGTC AGAATAAGCC CAGGTCAGAG
    GCAATTTGGA AGAAGTATTC AGAATGCGCC TTTTTCCAGG AATGTCAAAG AAAATTGAAA
    CACAGACTTG GTCTGGATTC CTATTTACTG AAACCAGTGC AACGTATCAC TAAATACCAG
    TTACTGTTGA AGGAGCTATT AAAATATAGC AAAGACTGTG TCGGATCTGT AGAATTAAAG
    GAGGCACTTG ACACAATGCT GGATTTACTG AAGTCAGTTA ATGACTTAAT GCATCAGACT
    GCAATAAATG GCTATATCGG AAACTTAAAT GAGCTGGGCA AGATGATAAT GCAGGGTGCG
    TTCAGTGTTT GGATTGGACA CAAGAAAGGT ACTACAAAGA TGAAGGATTT TGCTAGATTC
    AAACCAATGC AGCGGCACCT TTTCCTGTAT GAAAAAGCCG TCGTATTTTG TAAAAGGCGT
    GTTGAAAGTG GAGAAGGTGG TGATCGATAC CCATCATACA GTTTTAAGCA CTGTTTGAAA
    ATGGATGAAC TTGGAATCAC TGAATATGTA AAAGGAGATA ACCGCAAGTT TGAAATCTGG
    AATGCTGGAA AGGAAGAAGT TTATATTGTC CAGGCTTCAA ATATAGATAT GAAGATGATG
    TGGTTAAAAG AAATCAGAAC TATTTTGTTG AAGCAACAGG AACTTTTGAC AGTTAAGAAC
    CGAGAACAGC ATAATCAGTT AACAGAGCAG AATCATTTTT CTTCTCAGCA GAATGACGAA
    GGGCAACACA GAGGTTTTAT AGGCGCTGAG GAACCTGAGT TGGGACATAT CAGCACTATG
    GTGGAGGTCG GTGAGTCCAT CACATCAGTT CAAGCAGAAG CAAATACAGT TTGGACTGAG
    ATGTCATCAC CTGCAAAAGT CTCTGATGAA CCTGAGAAAT GGTCCAGTAA CTATTTCTAC
    TCCTCTTATG ATGGCCATGA AGAAGAAAGA CCTCTAATGT CTCCAGGCAA ATATAAAGCC
    CTAGCAGATT GCAGGAAGAG AGGCTCAGAA GACCTCCTGA TTAGAAATGG AGATGTCATT
    CAGCTTCTCC ATGAGGATGC TGAGGGTCGA TGGTTGGTGA AAAATCTAAA CAGAAGAAAA
    GAAGGTCTGA TTCCAGGGAA CAGTTTGCAG ATTTTAATCG GTGACTATAG GTTTCGGAAT
    GCCAAAGATG CTGCTCTGAG TAACAAGGAA ACTAAGTTCC CCTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325696.1
    CDS317..3187
    Translation

    Target ORF information:

    RefSeq Version XM_015470210.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470210.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGCTATAGAA
    AATTTTGCCC TCACAGTGAA AGATACGGCT CAGATGTTAC AGTCCTTTGG AACTGAACTG
    GCTGAGACAG AACTACCAGG TGACATTCCT GCAATGGAAG AAATTCTTGC AATTCGTGCT
    GAAAGGTACC ACCTGTTAAA GAAGGATATC ACAGCTGTAA CCAAAGAAGG AAAATTTCTG
    CTAACAAATC TGGAAGTGCC TGATGCTGAG GGTGCTGTGA GTTCAAGACT TGAAAATGAT
    CAGCCGACTA GTGGTGACTG GCAAACTATT AATAAATTGC TGACTCAAGT ACATGATATG
    GAAATAGCTT TTGATGGATT TTGGGAAAAA CACCAGCTAA AAATGGAGCA GTATTTGCAA
    CTCTGGAAGT TTGAGCAGGA TTTTCAAGAG CTCGTGAGTG AAGCTGAACT TATACTAAAT
    CAACAAGCGG AGTTGGCAGA TGTAACAGGG ACTATAGCTC AAGTGAAACA AAAAATTAAA
    AAGTTGGAAA ACTTGGATGA AAATTCTCAG GACCTGTTAG CAAAGGCCCG GTTCGTAATA
    ATACATGGAC ATAGGCTCGC AGCAAGTCAT CATTATGCCC TGGATTTGAT CTGTCAGAGG
    TGCAATGAAC TACGTTACCT TTCTGATATT TTGGTAAATG AAATCAGAAC CAAACGGATA
    CAGCTCAACA GGACCTTTAA AATGCATAAA CTCCTACAGC AGGCTCTCCA GTGCTGTGAT
    GAAGGAGAGT GCCTTCTGGC TAATCAAGAA ATAGACAAGT TTCAGTCTAA AGAAGATGCA
    CAGAAGGCCC TCCAAGACAT TGAAAATTTT CTTGAAATGG CTCTACCCTT TATAAATTAT
    GATCCTGAAA CCCTACAGTA TGAATTTGGT ATAATTTTAT CTCCTGAACT CAAGGTTCAA
    ATGCAGACCA TCCAACTCAA GCTTGAAAAC ATCCGAAGTA TATTTGAGAA CCAACAGGCT
    GGTTTCAGAA ACCTGACAGA TAAACATGTG AGGCCGATTC CGTTTGTAGT ATCTGCTTCT
    GACAATTTGG TCAGATCTGG AGCACCATTT TTTTCACCTA CACAAGGGAA GAAGACTTGG
    AGACAAAATC AGAGCAACAC AAAAACTGAA GCAGTGTATG ATTGTCAGGA GAAGAGAAGT
    TCTGCTCAGT CCTCCAATTT GGACAATGAC AATAGCTTGG ATATTTTAAA GAACCACGTC
    CTAAATGAGC TGATACAGAC GGAGAGGGCA TATGTTAGGG AACTGTTTAC TGTTTTGTTG
    GGCTATAGAG CGGAGATGGA TAATCCAGAG ATGTTTGTTC TTATGCCACC TCTCCTGAGA
    AGTAAAAGGG ATGTTCTCTT TGGAAACATG GCAGAAATAT ATGAATTCCA TAACAACATT
    TTCATGAGCA GTCTGGAAAA TTGTGTTGAT GCTCCAGAAA GAGTGGGATC TTGTTTCCTG
    GAAAGGAAAG ATGATTTTCA AATGTATGCG AAGTATTGTC AGAATAAGCC CAGGTCAGAG
    GCAATTTGGA AGAAGTATTC AGAATGCGCC TTTTTCCAGG AATGTCAAAG AAAATTGAAA
    CACAGACTTG GTCTGGATTC CTATTTACTG AAACCAGTGC AACGTATCAC TAAATACCAG
    TTACTGTTGA AGGAGCTATT AAAATATAGC AAAGACTGTG TCGGATCTGT AGAATTAAAG
    GAGGCACTTG ACACAATGCT GGATTTACTG AAGTCAGTTA ATGACTTAAT GCATCAGACT
    GCAATAAATG GCTATATCGG AAACTTAAAT GAGCTGGGCA AGATGATAAT GCAGGGTGCG
    TTCAGTGTTT GGATTGGACA CAAGAAAGGT ACTACAAAGA TGAAGGATTT TGCTAGATTC
    AAACCAATGC AGCGGCACCT TTTCCTGTAT GAAAAAGCCG TCGTATTTTG TAAAAGGCGT
    GTTGAAAGTG GAGAAGGTGG TGATCGATAC CCATCATACA GTTTTAAGCA CTGTTTGAAA
    ATGGATGAAC TTGGAATCAC TGAATATGTA AAAGGAGATA ACCGCAAGTT TGAAATCTGG
    AATGCTGGAA AGGAAGAAGT TTATATTGTC CAGGCTTCAA ATATAGATAT GAAGATGATG
    TGGTTAAAAG AAATCAGAAC TATTTTGTTG AAGCAACAGG AACTTTTGAC AGTTAAGAAC
    CGAGAACAGC ATAATCAGTT AACAGAGCAG AATCATTTTT CTTCTCAGCA GAATGACGAA
    GGGCAACACA GAGGTTTTAT AGGCGCTGAG GAACCTGAGT TGGGACATAT CAGCACTATG
    GTGGAGGTCG GTGAGTCCAT CACATCAGTT CAAGCAGAAG CAAATACAGT TTGGACTGAG
    ATGTCATCAC CTGCAAAAGT CTCTGATGAA CCTGAGAAAT GGTCCAGTAA CTATTTCTAC
    TCCTCTTATG ATGGCCATGA AGAAGAAAGA CCTCTAATGT CTCCAGGCAA ATATAAAGCC
    CTAGCAGATT GCAGGAAGAG AGGCTCAGAA GACCTCCTGA TTAGAAATGG AGATGTCATT
    CAGCTTCTCC ATGAGGATGC TGAGGGTCGA TGGTTGGTGA AAAATCTAAA CAGAAGAAAA
    GAAGGTCTGA TTCCAGGGAA CAGTTTGCAG ATTTTAATCG GTGACTATAG GTTTCGGAAT
    GCCAAAGATG CTGCTCTGAG TAACAAGGAA ACTAAGTTCC CCTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89237
    Clone ID Related Accession (Same CDS sequence) XM_015470210.1 , XM_015461455.1
    Accession Version XM_015461455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGCTATAGAA
    AATTTTGCCC TCACAGTGAA AGATACGGCT CAGATGTTAC AGTCCTTTGG AACTGAACTG
    GCTGAGACAG AACTACCAGG TGACATTCCT GCAATGGAAG AAATTCTTGC AATTCGTGCT
    GAAAGGTACC ACCTGTTAAA GAAGGATATC ACAGCTGTAA CCAAAGAAGG AAAATTTCTG
    CTAACAAATC TGGAAGTGCC TGATGCTGAG GGTGCTGTGA GTTCAAGACT TGAAAATGAT
    CAGCCGACTA GTGGTGACTG GCAAACTATT AATAAATTGC TGACTCAAGT ACATGATATG
    GAAATAGCTT TTGATGGATT TTGGGAAAAA CACCAGCTAA AAATGGAGCA GTATTTGCAA
    CTCTGGAAGT TTGAGCAGGA TTTTCAAGAG CTCGTGAGTG AAGCTGAACT TATACTAAAT
    CAACAAGCGG AGTTGGCAGA TGTAACAGGG ACTATAGCTC AAGTGAAACA AAAAATTAAA
    AAGTTGGAAA ACTTGGATGA AAATTCTCAG GACCTGTTAG CAAAGGCCCG GTTCGTAATA
    ATACATGGAC ATAGGCTCGC AGCAAGTCAT CATTATGCCC TGGATTTGAT CTGTCAGAGG
    TGCAATGAAC TACGTTACCT TTCTGATATT TTGGTAAATG AAATCAGAAC CAAACGGATA
    CAGCTCAACA GGACCTTTAA AATGCATAAA CTCCTACAGC AGGCTCTCCA GTGCTGTGAT
    GAAGGAGAGT GCCTTCTGGC TAATCAAGAA ATAGACAAGT TTCAGTCTAA AGAAGATGCA
    CAGAAGGCCC TCCAAGACAT TGAAAATTTT CTTGAAATGG CTCTACCCTT TATAAATTAT
    GATCCTGAAA CCCTACAGTA TGAATTTGGT ATAATTTTAT CTCCTGAACT CAAGGTTCAA
    ATGCAGACCA TCCAACTCAA GCTTGAAAAC ATCCGAAGTA TATTTGAGAA CCAACAGGCT
    GGTTTCAGAA ACCTGACAGA TAAACATGTG AGGCCGATTC CGTTTGTAGT ATCTGCTTCT
    GACAATTTGG TCAGATCTGG AGCACCATTT TTTTCACCTA CACAAGGGAA GAAGACTTGG
    AGACAAAATC AGAGCAACAC AAAAACTGAA GCAGTGTATG ATTGTCAGGA GAAGAGAAGT
    TCTGCTCAGT CCTCCAATTT GGACAATGAC AATAGCTTGG ATATTTTAAA GAACCACGTC
    CTAAATGAGC TGATACAGAC GGAGAGGGCA TATGTTAGGG AACTGTTTAC TGTTTTGTTG
    GGCTATAGAG CGGAGATGGA TAATCCAGAG ATGTTTGTTC TTATGCCACC TCTCCTGAGA
    AGTAAAAGGG ATGTTCTCTT TGGAAACATG GCAGAAATAT ATGAATTCCA TAACAACATT
    TTCATGAGCA GTCTGGAAAA TTGTGTTGAT GCTCCAGAAA GAGTGGGATC TTGTTTCCTG
    GAAAGGAAAG ATGATTTTCA AATGTATGCG AAGTATTGTC AGAATAAGCC CAGGTCAGAG
    GCAATTTGGA AGAAGTATTC AGAATGCGCC TTTTTCCAGG AATGTCAAAG AAAATTGAAA
    CACAGACTTG GTCTGGATTC CTATTTACTG AAACCAGTGC AACGTATCAC TAAATACCAG
    TTACTGTTGA AGGAGCTATT AAAATATAGC AAAGACTGTG TCGGATCTGT AGAATTAAAG
    GAGGCACTTG ACACAATGCT GGATTTACTG AAGTCAGTTA ATGACTTAAT GCATCAGACT
    GCAATAAATG GCTATATCGG AAACTTAAAT GAGCTGGGCA AGATGATAAT GCAGGGTGCG
    TTCAGTGTTT GGATTGGACA CAAGAAAGGT ACTACAAAGA TGAAGGATTT TGCTAGATTC
    AAACCAATGC AGCGGCACCT TTTCCTGTAT GAAAAAGCCG TCGTATTTTG TAAAAGGCGT
    GTTGAAAGTG GAGAAGGTGG TGATCGATAC CCATCATACA GTTTTAAGCA CTGTTTGAAA
    ATGGATGAAC TTGGAATCAC TGAATATGTA AAAGGAGATA ACCGCAAGTT TGAAATCTGG
    AATGCTGGAA AGGAAGAAGT TTATATTGTC CAGGCTTCAA ATATAGATAT GAAGATGATG
    TGGTTAAAAG AAATCAGAAC TATTTTGTTG AAGCAACAGG AACTTTTGAC AGTTAAGAAC
    CGAGAACAGC ATAATCAGTT AACAGAGCAG AATCATTTTT CTTCTCAGCA GAATGACGAA
    GGGCAACACA GAGGTTTTAT AGGCGCTGAG GAACCTGAGT TGGGACATAT CAGCACTATG
    GTGGAGGTCG GTGAGTCCAT CACATCAGTT CAAGCAGAAG CAAATACAGT TTGGACTGAG
    ATGTCATCAC CTGCAAAAGT CTCTGATGAA CCTGAGAAAT GGTCCAGTAA CTATTTCTAC
    TCCTCTTATG ATGGCCATGA AGAAGAAAGA CCTCTAATGT CTCCAGGCAA ATATAAAGCC
    CTAGCAGATT GCAGGAAGAG AGGCTCAGAA GACCTCCTGA TTAGAAATGG AGATGTCATT
    CAGCTTCTCC ATGAGGATGC TGAGGGTCGA TGGTTGGTGA AAAATCTAAA CAGAAGAAAA
    GAAGGTCTGA TTCCAGGGAA CAGTTTGCAG ATTTTAATCG GTGACTATAG GTTTCGGAAT
    GCCAAAGATG CTGCTCTGAG TAACAAGGAA ACTAAGTTCC CCTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316941.1
    CDS317..3187
    Translation

    Target ORF information:

    RefSeq Version XM_015461455.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGCTATAGAA
    AATTTTGCCC TCACAGTGAA AGATACGGCT CAGATGTTAC AGTCCTTTGG AACTGAACTG
    GCTGAGACAG AACTACCAGG TGACATTCCT GCAATGGAAG AAATTCTTGC AATTCGTGCT
    GAAAGGTACC ACCTGTTAAA GAAGGATATC ACAGCTGTAA CCAAAGAAGG AAAATTTCTG
    CTAACAAATC TGGAAGTGCC TGATGCTGAG GGTGCTGTGA GTTCAAGACT TGAAAATGAT
    CAGCCGACTA GTGGTGACTG GCAAACTATT AATAAATTGC TGACTCAAGT ACATGATATG
    GAAATAGCTT TTGATGGATT TTGGGAAAAA CACCAGCTAA AAATGGAGCA GTATTTGCAA
    CTCTGGAAGT TTGAGCAGGA TTTTCAAGAG CTCGTGAGTG AAGCTGAACT TATACTAAAT
    CAACAAGCGG AGTTGGCAGA TGTAACAGGG ACTATAGCTC AAGTGAAACA AAAAATTAAA
    AAGTTGGAAA ACTTGGATGA AAATTCTCAG GACCTGTTAG CAAAGGCCCG GTTCGTAATA
    ATACATGGAC ATAGGCTCGC AGCAAGTCAT CATTATGCCC TGGATTTGAT CTGTCAGAGG
    TGCAATGAAC TACGTTACCT TTCTGATATT TTGGTAAATG AAATCAGAAC CAAACGGATA
    CAGCTCAACA GGACCTTTAA AATGCATAAA CTCCTACAGC AGGCTCTCCA GTGCTGTGAT
    GAAGGAGAGT GCCTTCTGGC TAATCAAGAA ATAGACAAGT TTCAGTCTAA AGAAGATGCA
    CAGAAGGCCC TCCAAGACAT TGAAAATTTT CTTGAAATGG CTCTACCCTT TATAAATTAT
    GATCCTGAAA CCCTACAGTA TGAATTTGGT ATAATTTTAT CTCCTGAACT CAAGGTTCAA
    ATGCAGACCA TCCAACTCAA GCTTGAAAAC ATCCGAAGTA TATTTGAGAA CCAACAGGCT
    GGTTTCAGAA ACCTGACAGA TAAACATGTG AGGCCGATTC CGTTTGTAGT ATCTGCTTCT
    GACAATTTGG TCAGATCTGG AGCACCATTT TTTTCACCTA CACAAGGGAA GAAGACTTGG
    AGACAAAATC AGAGCAACAC AAAAACTGAA GCAGTGTATG ATTGTCAGGA GAAGAGAAGT
    TCTGCTCAGT CCTCCAATTT GGACAATGAC AATAGCTTGG ATATTTTAAA GAACCACGTC
    CTAAATGAGC TGATACAGAC GGAGAGGGCA TATGTTAGGG AACTGTTTAC TGTTTTGTTG
    GGCTATAGAG CGGAGATGGA TAATCCAGAG ATGTTTGTTC TTATGCCACC TCTCCTGAGA
    AGTAAAAGGG ATGTTCTCTT TGGAAACATG GCAGAAATAT ATGAATTCCA TAACAACATT
    TTCATGAGCA GTCTGGAAAA TTGTGTTGAT GCTCCAGAAA GAGTGGGATC TTGTTTCCTG
    GAAAGGAAAG ATGATTTTCA AATGTATGCG AAGTATTGTC AGAATAAGCC CAGGTCAGAG
    GCAATTTGGA AGAAGTATTC AGAATGCGCC TTTTTCCAGG AATGTCAAAG AAAATTGAAA
    CACAGACTTG GTCTGGATTC CTATTTACTG AAACCAGTGC AACGTATCAC TAAATACCAG
    TTACTGTTGA AGGAGCTATT AAAATATAGC AAAGACTGTG TCGGATCTGT AGAATTAAAG
    GAGGCACTTG ACACAATGCT GGATTTACTG AAGTCAGTTA ATGACTTAAT GCATCAGACT
    GCAATAAATG GCTATATCGG AAACTTAAAT GAGCTGGGCA AGATGATAAT GCAGGGTGCG
    TTCAGTGTTT GGATTGGACA CAAGAAAGGT ACTACAAAGA TGAAGGATTT TGCTAGATTC
    AAACCAATGC AGCGGCACCT TTTCCTGTAT GAAAAAGCCG TCGTATTTTG TAAAAGGCGT
    GTTGAAAGTG GAGAAGGTGG TGATCGATAC CCATCATACA GTTTTAAGCA CTGTTTGAAA
    ATGGATGAAC TTGGAATCAC TGAATATGTA AAAGGAGATA ACCGCAAGTT TGAAATCTGG
    AATGCTGGAA AGGAAGAAGT TTATATTGTC CAGGCTTCAA ATATAGATAT GAAGATGATG
    TGGTTAAAAG AAATCAGAAC TATTTTGTTG AAGCAACAGG AACTTTTGAC AGTTAAGAAC
    CGAGAACAGC ATAATCAGTT AACAGAGCAG AATCATTTTT CTTCTCAGCA GAATGACGAA
    GGGCAACACA GAGGTTTTAT AGGCGCTGAG GAACCTGAGT TGGGACATAT CAGCACTATG
    GTGGAGGTCG GTGAGTCCAT CACATCAGTT CAAGCAGAAG CAAATACAGT TTGGACTGAG
    ATGTCATCAC CTGCAAAAGT CTCTGATGAA CCTGAGAAAT GGTCCAGTAA CTATTTCTAC
    TCCTCTTATG ATGGCCATGA AGAAGAAAGA CCTCTAATGT CTCCAGGCAA ATATAAAGCC
    CTAGCAGATT GCAGGAAGAG AGGCTCAGAA GACCTCCTGA TTAGAAATGG AGATGTCATT
    CAGCTTCTCC ATGAGGATGC TGAGGGTCGA TGGTTGGTGA AAAATCTAAA CAGAAGAAAA
    GAAGGTCTGA TTCCAGGGAA CAGTTTGCAG ATTTTAATCG GTGACTATAG GTTTCGGAAT
    GCCAAAGATG CTGCTCTGAG TAACAAGGAA ACTAAGTTCC CCTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238713
    Clone ID Related Accession (Same CDS sequence) XM_024988613.1
    Accession Version XM_024988613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: proto-oncogene DBL
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGATC TGACTCCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGTCTCCAG GCAAATATAA AGCCCTAGCA
    GATTGCAGGA AGAGAGGCTC AGAAGACCTC CTGATTAGAA ATGGAGATGT CATTCAGCTT
    CTCCATGAGG ATGCTGAGGG TCGATGGTTG GTGAAAAATC TAAACAGAAG AAAAGAAGGT
    CTGATTCCAG GGAACAGTTT GCAGATTTTA ATCGGTGACT ATAGGTTTCG GAATGCCAAA
    GGTACAGGTA TAATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844381.1
    CDS167..3484
    Translation

    Target ORF information:

    RefSeq Version XM_024988613.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGATC TGACTCCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGTCTCCAG GCAAATATAA AGCCCTAGCA
    GATTGCAGGA AGAGAGGCTC AGAAGACCTC CTGATTAGAA ATGGAGATGT CATTCAGCTT
    CTCCATGAGG ATGCTGAGGG TCGATGGTTG GTGAAAAATC TAAACAGAAG AAAAGAAGGT
    CTGATTCCAG GGAACAGTTT GCAGATTTTA ATCGGTGACT ATAGGTTTCG GAATGCCAAA
    GGTACAGGTA TAATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89233
    Clone ID Related Accession (Same CDS sequence) XM_015470206.1 , XM_015461451.1
    Accession Version XM_015470206.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGGGAAGAAG
    ACTTGGAGAC AAAATCAGAG CAACACAAAA ACTGAAGCAG TGTATGATTG TCAGGAGAAG
    AGAAGTTCTG CTCAGTCCTC CAATTTGGAC AATGACAATA GCTTGGATAT TTTAAAGAAC
    CACGTCCTAA ATGAGCTGAT ACAGACGGAG AGGGCATATG TTAGGGAACT GTTTACTGTT
    TTGTTGGGCT ATAGAGCGGA GATGGATAAT CCAGAGATGT TTGTTCTTAT GCCACCTCTC
    CTGAGAAGTA AAAGGGATGT TCTCTTTGGA AACATGGCAG AAATATATGA ATTCCATAAC
    AACATTTTCA TGAGCAGTCT GGAAAATTGT GTTGATGCTC CAGAAAGAGT GGGATCTTGT
    TTCCTGGAAA GGAAAGATGA TTTTCAAATG TATGCGAAGT ATTGTCAGAA TAAGCCCAGG
    TCAGAGGCAA TTTGGAAGAA GTATTCAGAA TGCGCCTTTT TCCAGGAATG TCAAAGAAAA
    TTGAAACACA GACTTGGTCT GGATTCCTAT TTACTGAAAC CAGTGCAACG TATCACTAAA
    TACCAGTTAC TGTTGAAGGA GCTATTAAAA TATAGCAAAG ACTGTGTCGG ATCTGTAGAA
    TTAAAGGAGG CACTTGACAC AATGCTGGAT TTACTGAAGT CAGTTAATGA CTTAATGCAT
    CAGACTGCAA TAAATGGCTA TATCGGAAAC TTAAATGAGC TGGGCAAGAT GATAATGCAG
    GGTGCGTTCA GTGTTTGGAT TGGACACAAG AAAGGTACTA CAAAGATGAA GGATTTTGCT
    AGATTCAAAC CAATGCAGCG GCACCTTTTC CTGTATGAAA AAGCCGTCGT ATTTTGTAAA
    AGGCGTGTTG AAAGTGGAGA AGGTGGTGAT CGATACCCAT CATACAGTTT TAAGCACTGT
    TTGAAAATGG ATGAACTTGG AATCACTGAA TATGTAAAAG GAGATAACCG CAAGTTTGAA
    ATCTGGAATG CTGGAAAGGA AGAAGTTTAT ATTGTCCAGG CTTCAAATAT AGATATGAAG
    ATGATGTGGT TAAAAGAAAT CAGAACTATT TTGTTGAAGC AACAGGAACT TTTGACAGTT
    AAGAACCGAG AACAGCATAA TCAGTTAACA GAGCAGAATC ATTTTTCTTC TCAGCAGAAT
    GACGAAGGGC AACACAGAGG TTTTATAGGC GCTGAGGAAC CTGAGTTGGG ACATATCAGC
    ACTATGGTGG AGGTCGGTGA GTCCATCACA TCAGTTCAAG CAGAAGCAAA TACAGTTTGG
    ACTGAGATGT CATCACCTGC AAAAGTCTCT GATGAACCTG AGAAATGGTC CAGTAACTAT
    TTCTACTCCT CTTATGATGG CCATGAAGAA GAAAGACCTC TAATGTCTCC AGGCAAATAT
    AAAGCCCTAG CAGATTGCAG GAAGAGAGGC TCAGAAGACC TCCTGATTAG AAATGGAGAT
    GTCATTCAGC TTCTCCATGA GGATGCTGAG GGTCGATGGT TGGTGAAAAA TCTAAACAGA
    AGAAAAGAAG GTCTGATTCC AGGGAACAGT TTGCAGATTT TAATCGGTGA CTATAGGTTT
    CGGAATGCCA AAGATGCTGC TCTGAGTAAC AAGGAAACTA AGTTCCCCTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325692.1
    CDS116..3412
    Translation

    Target ORF information:

    RefSeq Version XM_015470206.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGGGAAGAAG
    ACTTGGAGAC AAAATCAGAG CAACACAAAA ACTGAAGCAG TGTATGATTG TCAGGAGAAG
    AGAAGTTCTG CTCAGTCCTC CAATTTGGAC AATGACAATA GCTTGGATAT TTTAAAGAAC
    CACGTCCTAA ATGAGCTGAT ACAGACGGAG AGGGCATATG TTAGGGAACT GTTTACTGTT
    TTGTTGGGCT ATAGAGCGGA GATGGATAAT CCAGAGATGT TTGTTCTTAT GCCACCTCTC
    CTGAGAAGTA AAAGGGATGT TCTCTTTGGA AACATGGCAG AAATATATGA ATTCCATAAC
    AACATTTTCA TGAGCAGTCT GGAAAATTGT GTTGATGCTC CAGAAAGAGT GGGATCTTGT
    TTCCTGGAAA GGAAAGATGA TTTTCAAATG TATGCGAAGT ATTGTCAGAA TAAGCCCAGG
    TCAGAGGCAA TTTGGAAGAA GTATTCAGAA TGCGCCTTTT TCCAGGAATG TCAAAGAAAA
    TTGAAACACA GACTTGGTCT GGATTCCTAT TTACTGAAAC CAGTGCAACG TATCACTAAA
    TACCAGTTAC TGTTGAAGGA GCTATTAAAA TATAGCAAAG ACTGTGTCGG ATCTGTAGAA
    TTAAAGGAGG CACTTGACAC AATGCTGGAT TTACTGAAGT CAGTTAATGA CTTAATGCAT
    CAGACTGCAA TAAATGGCTA TATCGGAAAC TTAAATGAGC TGGGCAAGAT GATAATGCAG
    GGTGCGTTCA GTGTTTGGAT TGGACACAAG AAAGGTACTA CAAAGATGAA GGATTTTGCT
    AGATTCAAAC CAATGCAGCG GCACCTTTTC CTGTATGAAA AAGCCGTCGT ATTTTGTAAA
    AGGCGTGTTG AAAGTGGAGA AGGTGGTGAT CGATACCCAT CATACAGTTT TAAGCACTGT
    TTGAAAATGG ATGAACTTGG AATCACTGAA TATGTAAAAG GAGATAACCG CAAGTTTGAA
    ATCTGGAATG CTGGAAAGGA AGAAGTTTAT ATTGTCCAGG CTTCAAATAT AGATATGAAG
    ATGATGTGGT TAAAAGAAAT CAGAACTATT TTGTTGAAGC AACAGGAACT TTTGACAGTT
    AAGAACCGAG AACAGCATAA TCAGTTAACA GAGCAGAATC ATTTTTCTTC TCAGCAGAAT
    GACGAAGGGC AACACAGAGG TTTTATAGGC GCTGAGGAAC CTGAGTTGGG ACATATCAGC
    ACTATGGTGG AGGTCGGTGA GTCCATCACA TCAGTTCAAG CAGAAGCAAA TACAGTTTGG
    ACTGAGATGT CATCACCTGC AAAAGTCTCT GATGAACCTG AGAAATGGTC CAGTAACTAT
    TTCTACTCCT CTTATGATGG CCATGAAGAA GAAAGACCTC TAATGTCTCC AGGCAAATAT
    AAAGCCCTAG CAGATTGCAG GAAGAGAGGC TCAGAAGACC TCCTGATTAG AAATGGAGAT
    GTCATTCAGC TTCTCCATGA GGATGCTGAG GGTCGATGGT TGGTGAAAAA TCTAAACAGA
    AGAAAAGAAG GTCTGATTCC AGGGAACAGT TTGCAGATTT TAATCGGTGA CTATAGGTTT
    CGGAATGCCA AAGATGCTGC TCTGAGTAAC AAGGAAACTA AGTTCCCCTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89233
    Clone ID Related Accession (Same CDS sequence) XM_015470206.1 , XM_015461451.1
    Accession Version XM_015461451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGGGAAGAAG
    ACTTGGAGAC AAAATCAGAG CAACACAAAA ACTGAAGCAG TGTATGATTG TCAGGAGAAG
    AGAAGTTCTG CTCAGTCCTC CAATTTGGAC AATGACAATA GCTTGGATAT TTTAAAGAAC
    CACGTCCTAA ATGAGCTGAT ACAGACGGAG AGGGCATATG TTAGGGAACT GTTTACTGTT
    TTGTTGGGCT ATAGAGCGGA GATGGATAAT CCAGAGATGT TTGTTCTTAT GCCACCTCTC
    CTGAGAAGTA AAAGGGATGT TCTCTTTGGA AACATGGCAG AAATATATGA ATTCCATAAC
    AACATTTTCA TGAGCAGTCT GGAAAATTGT GTTGATGCTC CAGAAAGAGT GGGATCTTGT
    TTCCTGGAAA GGAAAGATGA TTTTCAAATG TATGCGAAGT ATTGTCAGAA TAAGCCCAGG
    TCAGAGGCAA TTTGGAAGAA GTATTCAGAA TGCGCCTTTT TCCAGGAATG TCAAAGAAAA
    TTGAAACACA GACTTGGTCT GGATTCCTAT TTACTGAAAC CAGTGCAACG TATCACTAAA
    TACCAGTTAC TGTTGAAGGA GCTATTAAAA TATAGCAAAG ACTGTGTCGG ATCTGTAGAA
    TTAAAGGAGG CACTTGACAC AATGCTGGAT TTACTGAAGT CAGTTAATGA CTTAATGCAT
    CAGACTGCAA TAAATGGCTA TATCGGAAAC TTAAATGAGC TGGGCAAGAT GATAATGCAG
    GGTGCGTTCA GTGTTTGGAT TGGACACAAG AAAGGTACTA CAAAGATGAA GGATTTTGCT
    AGATTCAAAC CAATGCAGCG GCACCTTTTC CTGTATGAAA AAGCCGTCGT ATTTTGTAAA
    AGGCGTGTTG AAAGTGGAGA AGGTGGTGAT CGATACCCAT CATACAGTTT TAAGCACTGT
    TTGAAAATGG ATGAACTTGG AATCACTGAA TATGTAAAAG GAGATAACCG CAAGTTTGAA
    ATCTGGAATG CTGGAAAGGA AGAAGTTTAT ATTGTCCAGG CTTCAAATAT AGATATGAAG
    ATGATGTGGT TAAAAGAAAT CAGAACTATT TTGTTGAAGC AACAGGAACT TTTGACAGTT
    AAGAACCGAG AACAGCATAA TCAGTTAACA GAGCAGAATC ATTTTTCTTC TCAGCAGAAT
    GACGAAGGGC AACACAGAGG TTTTATAGGC GCTGAGGAAC CTGAGTTGGG ACATATCAGC
    ACTATGGTGG AGGTCGGTGA GTCCATCACA TCAGTTCAAG CAGAAGCAAA TACAGTTTGG
    ACTGAGATGT CATCACCTGC AAAAGTCTCT GATGAACCTG AGAAATGGTC CAGTAACTAT
    TTCTACTCCT CTTATGATGG CCATGAAGAA GAAAGACCTC TAATGTCTCC AGGCAAATAT
    AAAGCCCTAG CAGATTGCAG GAAGAGAGGC TCAGAAGACC TCCTGATTAG AAATGGAGAT
    GTCATTCAGC TTCTCCATGA GGATGCTGAG GGTCGATGGT TGGTGAAAAA TCTAAACAGA
    AGAAAAGAAG GTCTGATTCC AGGGAACAGT TTGCAGATTT TAATCGGTGA CTATAGGTTT
    CGGAATGCCA AAGATGCTGC TCTGAGTAAC AAGGAAACTA AGTTCCCCTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316937.1
    CDS116..3412
    Translation

    Target ORF information:

    RefSeq Version XM_015461451.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGGGAAGAAG
    ACTTGGAGAC AAAATCAGAG CAACACAAAA ACTGAAGCAG TGTATGATTG TCAGGAGAAG
    AGAAGTTCTG CTCAGTCCTC CAATTTGGAC AATGACAATA GCTTGGATAT TTTAAAGAAC
    CACGTCCTAA ATGAGCTGAT ACAGACGGAG AGGGCATATG TTAGGGAACT GTTTACTGTT
    TTGTTGGGCT ATAGAGCGGA GATGGATAAT CCAGAGATGT TTGTTCTTAT GCCACCTCTC
    CTGAGAAGTA AAAGGGATGT TCTCTTTGGA AACATGGCAG AAATATATGA ATTCCATAAC
    AACATTTTCA TGAGCAGTCT GGAAAATTGT GTTGATGCTC CAGAAAGAGT GGGATCTTGT
    TTCCTGGAAA GGAAAGATGA TTTTCAAATG TATGCGAAGT ATTGTCAGAA TAAGCCCAGG
    TCAGAGGCAA TTTGGAAGAA GTATTCAGAA TGCGCCTTTT TCCAGGAATG TCAAAGAAAA
    TTGAAACACA GACTTGGTCT GGATTCCTAT TTACTGAAAC CAGTGCAACG TATCACTAAA
    TACCAGTTAC TGTTGAAGGA GCTATTAAAA TATAGCAAAG ACTGTGTCGG ATCTGTAGAA
    TTAAAGGAGG CACTTGACAC AATGCTGGAT TTACTGAAGT CAGTTAATGA CTTAATGCAT
    CAGACTGCAA TAAATGGCTA TATCGGAAAC TTAAATGAGC TGGGCAAGAT GATAATGCAG
    GGTGCGTTCA GTGTTTGGAT TGGACACAAG AAAGGTACTA CAAAGATGAA GGATTTTGCT
    AGATTCAAAC CAATGCAGCG GCACCTTTTC CTGTATGAAA AAGCCGTCGT ATTTTGTAAA
    AGGCGTGTTG AAAGTGGAGA AGGTGGTGAT CGATACCCAT CATACAGTTT TAAGCACTGT
    TTGAAAATGG ATGAACTTGG AATCACTGAA TATGTAAAAG GAGATAACCG CAAGTTTGAA
    ATCTGGAATG CTGGAAAGGA AGAAGTTTAT ATTGTCCAGG CTTCAAATAT AGATATGAAG
    ATGATGTGGT TAAAAGAAAT CAGAACTATT TTGTTGAAGC AACAGGAACT TTTGACAGTT
    AAGAACCGAG AACAGCATAA TCAGTTAACA GAGCAGAATC ATTTTTCTTC TCAGCAGAAT
    GACGAAGGGC AACACAGAGG TTTTATAGGC GCTGAGGAAC CTGAGTTGGG ACATATCAGC
    ACTATGGTGG AGGTCGGTGA GTCCATCACA TCAGTTCAAG CAGAAGCAAA TACAGTTTGG
    ACTGAGATGT CATCACCTGC AAAAGTCTCT GATGAACCTG AGAAATGGTC CAGTAACTAT
    TTCTACTCCT CTTATGATGG CCATGAAGAA GAAAGACCTC TAATGTCTCC AGGCAAATAT
    AAAGCCCTAG CAGATTGCAG GAAGAGAGGC TCAGAAGACC TCCTGATTAG AAATGGAGAT
    GTCATTCAGC TTCTCCATGA GGATGCTGAG GGTCGATGGT TGGTGAAAAA TCTAAACAGA
    AGAAAAGAAG GTCTGATTCC AGGGAACAGT TTGCAGATTT TAATCGGTGA CTATAGGTTT
    CGGAATGCCA AAGATGCTGC TCTGAGTAAC AAGGAAACTA AGTTCCCCTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89234
    Clone ID Related Accession (Same CDS sequence) XM_015461452.1 , XM_015470207.1
    Accession Version XM_015461452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GCTATAGAAA ATTTTGCCCT CACAGTGAAA GATACGGCTC AGATGTTACA GTCCTTTGGA
    ACTGAACTGG CTGAGACAGA ACTACCAGGT GACATTCCTG CAATGGAAGA AATTCTTGCA
    ATTCGTGCTG AAAGGTACCA CCTGTTAAAG AAGGATATCA CAGCTGTAAC CAAAGAAGGA
    AAATTTCTGC TAACAAATCT GGAAGTGCCT GATGCTGAGG GTGCTGTGAG TTCAAGACTT
    GAAAATGATC AGCCGACTAG TGGTGACTGG CAAACTATTA ATAAATTGCT GACTCAAGTA
    CATGATATGG AAATAGCTTT TGATGGATTT TGGGAAAAAC ACCAGCTAAA AATGGAGCAG
    TATTTGCAAC TCTGGAAGTT TGAGCAGGAT TTTCAAGAGC TCGTGAGTGA AGCTGAACTT
    ATACTAAATC AACAAGCGGA GTTGGCAGAT GTAACAGGGA CTATAGCTCA AGTGAAACAA
    AAAATTAAAA AGTTGGAAAA CTTGGATGAA AATTCTCAGG ACCTGTTAGC AAAGGCCCGG
    TTCGTAATAA TACATGGACA TAGGCTCGCA GCAAGTCATC ATTATGCCCT GGATTTGATC
    TGTCAGAGGT GCAATGAACT ACGTTACCTT TCTGATATTT TGGTAAATGA AATCAGAACC
    AAACGGATAC AGCTCAACAG GACCTTTAAA ATGCATAAAC TCCTACAGCA GGCTCTCCAG
    TGCTGTGATG AAGGAGAGTG CCTTCTGGCT AATCAAGAAA TAGACAAGTT TCAGTCTAAA
    GAAGATGCAC AGAAGGCCCT CCAAGACATT GAAAATTTTC TTGAAATGGC TCTACCCTTT
    ATAAATTATG ATCCTGAAAC CCTACAGTAT GAATTTGGTA TAATTTTATC TCCTGAACTC
    AAGGTTCAAA TGCAGACCAT CCAACTCAAG CTTGAAAACA TCCGAAGTAT ATTTGAGAAC
    CAACAGGCTG GTTTCAGAAA CCTGACAGAT AAACATGTGA GGCCGATTCC GTTTGTAGTA
    TCTGCTTCTG ACAATTTGGT CAGATCTGGA GCACCATTTT TTTCACCTAC ACAAGTGGGA
    GTTGGATATT CTTTCTTTCA AGCATGTAAA CTTTTTTCAA AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316938.1
    CDS117..3344
    Translation

    Target ORF information:

    RefSeq Version XM_015461452.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GCTATAGAAA ATTTTGCCCT CACAGTGAAA GATACGGCTC AGATGTTACA GTCCTTTGGA
    ACTGAACTGG CTGAGACAGA ACTACCAGGT GACATTCCTG CAATGGAAGA AATTCTTGCA
    ATTCGTGCTG AAAGGTACCA CCTGTTAAAG AAGGATATCA CAGCTGTAAC CAAAGAAGGA
    AAATTTCTGC TAACAAATCT GGAAGTGCCT GATGCTGAGG GTGCTGTGAG TTCAAGACTT
    GAAAATGATC AGCCGACTAG TGGTGACTGG CAAACTATTA ATAAATTGCT GACTCAAGTA
    CATGATATGG AAATAGCTTT TGATGGATTT TGGGAAAAAC ACCAGCTAAA AATGGAGCAG
    TATTTGCAAC TCTGGAAGTT TGAGCAGGAT TTTCAAGAGC TCGTGAGTGA AGCTGAACTT
    ATACTAAATC AACAAGCGGA GTTGGCAGAT GTAACAGGGA CTATAGCTCA AGTGAAACAA
    AAAATTAAAA AGTTGGAAAA CTTGGATGAA AATTCTCAGG ACCTGTTAGC AAAGGCCCGG
    TTCGTAATAA TACATGGACA TAGGCTCGCA GCAAGTCATC ATTATGCCCT GGATTTGATC
    TGTCAGAGGT GCAATGAACT ACGTTACCTT TCTGATATTT TGGTAAATGA AATCAGAACC
    AAACGGATAC AGCTCAACAG GACCTTTAAA ATGCATAAAC TCCTACAGCA GGCTCTCCAG
    TGCTGTGATG AAGGAGAGTG CCTTCTGGCT AATCAAGAAA TAGACAAGTT TCAGTCTAAA
    GAAGATGCAC AGAAGGCCCT CCAAGACATT GAAAATTTTC TTGAAATGGC TCTACCCTTT
    ATAAATTATG ATCCTGAAAC CCTACAGTAT GAATTTGGTA TAATTTTATC TCCTGAACTC
    AAGGTTCAAA TGCAGACCAT CCAACTCAAG CTTGAAAACA TCCGAAGTAT ATTTGAGAAC
    CAACAGGCTG GTTTCAGAAA CCTGACAGAT AAACATGTGA GGCCGATTCC GTTTGTAGTA
    TCTGCTTCTG ACAATTTGGT CAGATCTGGA GCACCATTTT TTTCACCTAC ACAAGTGGGA
    GTTGGATATT CTTTCTTTCA AGCATGTAAA CTTTTTTCAA AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89234
    Clone ID Related Accession (Same CDS sequence) XM_015461452.1 , XM_015470207.1
    Accession Version XM_015470207.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GCTATAGAAA ATTTTGCCCT CACAGTGAAA GATACGGCTC AGATGTTACA GTCCTTTGGA
    ACTGAACTGG CTGAGACAGA ACTACCAGGT GACATTCCTG CAATGGAAGA AATTCTTGCA
    ATTCGTGCTG AAAGGTACCA CCTGTTAAAG AAGGATATCA CAGCTGTAAC CAAAGAAGGA
    AAATTTCTGC TAACAAATCT GGAAGTGCCT GATGCTGAGG GTGCTGTGAG TTCAAGACTT
    GAAAATGATC AGCCGACTAG TGGTGACTGG CAAACTATTA ATAAATTGCT GACTCAAGTA
    CATGATATGG AAATAGCTTT TGATGGATTT TGGGAAAAAC ACCAGCTAAA AATGGAGCAG
    TATTTGCAAC TCTGGAAGTT TGAGCAGGAT TTTCAAGAGC TCGTGAGTGA AGCTGAACTT
    ATACTAAATC AACAAGCGGA GTTGGCAGAT GTAACAGGGA CTATAGCTCA AGTGAAACAA
    AAAATTAAAA AGTTGGAAAA CTTGGATGAA AATTCTCAGG ACCTGTTAGC AAAGGCCCGG
    TTCGTAATAA TACATGGACA TAGGCTCGCA GCAAGTCATC ATTATGCCCT GGATTTGATC
    TGTCAGAGGT GCAATGAACT ACGTTACCTT TCTGATATTT TGGTAAATGA AATCAGAACC
    AAACGGATAC AGCTCAACAG GACCTTTAAA ATGCATAAAC TCCTACAGCA GGCTCTCCAG
    TGCTGTGATG AAGGAGAGTG CCTTCTGGCT AATCAAGAAA TAGACAAGTT TCAGTCTAAA
    GAAGATGCAC AGAAGGCCCT CCAAGACATT GAAAATTTTC TTGAAATGGC TCTACCCTTT
    ATAAATTATG ATCCTGAAAC CCTACAGTAT GAATTTGGTA TAATTTTATC TCCTGAACTC
    AAGGTTCAAA TGCAGACCAT CCAACTCAAG CTTGAAAACA TCCGAAGTAT ATTTGAGAAC
    CAACAGGCTG GTTTCAGAAA CCTGACAGAT AAACATGTGA GGCCGATTCC GTTTGTAGTA
    TCTGCTTCTG ACAATTTGGT CAGATCTGGA GCACCATTTT TTTCACCTAC ACAAGTGGGA
    GTTGGATATT CTTTCTTTCA AGCATGTAAA CTTTTTTCAA AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325693.1
    CDS117..3344
    Translation

    Target ORF information:

    RefSeq Version XM_015470207.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GCTATAGAAA ATTTTGCCCT CACAGTGAAA GATACGGCTC AGATGTTACA GTCCTTTGGA
    ACTGAACTGG CTGAGACAGA ACTACCAGGT GACATTCCTG CAATGGAAGA AATTCTTGCA
    ATTCGTGCTG AAAGGTACCA CCTGTTAAAG AAGGATATCA CAGCTGTAAC CAAAGAAGGA
    AAATTTCTGC TAACAAATCT GGAAGTGCCT GATGCTGAGG GTGCTGTGAG TTCAAGACTT
    GAAAATGATC AGCCGACTAG TGGTGACTGG CAAACTATTA ATAAATTGCT GACTCAAGTA
    CATGATATGG AAATAGCTTT TGATGGATTT TGGGAAAAAC ACCAGCTAAA AATGGAGCAG
    TATTTGCAAC TCTGGAAGTT TGAGCAGGAT TTTCAAGAGC TCGTGAGTGA AGCTGAACTT
    ATACTAAATC AACAAGCGGA GTTGGCAGAT GTAACAGGGA CTATAGCTCA AGTGAAACAA
    AAAATTAAAA AGTTGGAAAA CTTGGATGAA AATTCTCAGG ACCTGTTAGC AAAGGCCCGG
    TTCGTAATAA TACATGGACA TAGGCTCGCA GCAAGTCATC ATTATGCCCT GGATTTGATC
    TGTCAGAGGT GCAATGAACT ACGTTACCTT TCTGATATTT TGGTAAATGA AATCAGAACC
    AAACGGATAC AGCTCAACAG GACCTTTAAA ATGCATAAAC TCCTACAGCA GGCTCTCCAG
    TGCTGTGATG AAGGAGAGTG CCTTCTGGCT AATCAAGAAA TAGACAAGTT TCAGTCTAAA
    GAAGATGCAC AGAAGGCCCT CCAAGACATT GAAAATTTTC TTGAAATGGC TCTACCCTTT
    ATAAATTATG ATCCTGAAAC CCTACAGTAT GAATTTGGTA TAATTTTATC TCCTGAACTC
    AAGGTTCAAA TGCAGACCAT CCAACTCAAG CTTGAAAACA TCCGAAGTAT ATTTGAGAAC
    CAACAGGCTG GTTTCAGAAA CCTGACAGAT AAACATGTGA GGCCGATTCC GTTTGTAGTA
    TCTGCTTCTG ACAATTTGGT CAGATCTGGA GCACCATTTT TTTCACCTAC ACAAGTGGGA
    GTTGGATATT CTTTCTTTCA AGCATGTAAA CTTTTTTCAA AAGGGAAGAA GACTTGGAGA
    CAAAATCAGA GCAACACAAA AACTGAAGCA GTGTATGATT GTCAGGAGAA GAGAAGTTCT
    GCTCAGTCCT CCAATTTGGA CAATGACAAT AGCTTGGATA TTTTAAAGAA CCACGTCCTA
    AATGAGCTGA TACAGACGGA GAGGGCATAT GTTAGGGAAC TGTTTACTGT TTTGTTGGGC
    TATAGAGCGG AGATGGATAA TCCAGAGATG TTTGTTCTTA TGCCACCTCT CCTGAGAAGT
    AAAAGGGATG TTCTCTTTGG AAACATGGCA GAAATATATG AATTCCATAA CAACATTTTC
    ATGAGCAGTC TGGAAAATTG TGTTGATGCT CCAGAAAGAG TGGGATCTTG TTTCCTGGAA
    AGGAAAGATG ATTTTCAAAT GTATGCGAAG TATTGTCAGA ATAAGCCCAG GTCAGAGGCA
    ATTTGGAAGA AGTATTCAGA ATGCGCCTTT TTCCAGGAAT GTCAAAGAAA ATTGAAACAC
    AGACTTGGTC TGGATTCCTA TTTACTGAAA CCAGTGCAAC GTATCACTAA ATACCAGTTA
    CTGTTGAAGG AGCTATTAAA ATATAGCAAA GACTGTGTCG GATCTGTAGA ATTAAAGGAG
    GCACTTGACA CAATGCTGGA TTTACTGAAG TCAGTTAATG ACTTAATGCA TCAGACTGCA
    ATAAATGGCT ATATCGGAAA CTTAAATGAG CTGGGCAAGA TGATAATGCA GGGTGCGTTC
    AGTGTTTGGA TTGGACACAA GAAAGGTACT ACAAAGATGA AGGATTTTGC TAGATTCAAA
    CCAATGCAGC GGCACCTTTT CCTGTATGAA AAAGCCGTCG TATTTTGTAA AAGGCGTGTT
    GAAAGTGGAG AAGGTGGTGA TCGATACCCA TCATACAGTT TTAAGCACTG TTTGAAAATG
    GATGAACTTG GAATCACTGA ATATGTAAAA GGAGATAACC GCAAGTTTGA AATCTGGAAT
    GCTGGAAAGG AAGAAGTTTA TATTGTCCAG GCTTCAAATA TAGATATGAA GATGATGTGG
    TTAAAAGAAA TCAGAACTAT TTTGTTGAAG CAACAGGAAC TTTTGACAGT TAAGAACCGA
    GAACAGCATA ATCAGTTAAC AGAGCAGAAT CATTTTTCTT CTCAGCAGAA TGACGAAGGG
    CAACACAGAG GTTTTATAGG CGCTGAGGAA CCTGAGTTGG GACATATCAG CACTATGGTG
    GAGGTCGGTG AGTCCATCAC ATCAGTTCAA GCAGAAGCAA ATACAGTTTG GACTGAGATG
    TCATCACCTG CAAAAGTCTC TGATGAACCT GAGAAATGGT CCAGTAACTA TTTCTACTCC
    TCTTATGATG GCCATGAAGA AGAAAGACCT CTAATGTCTC CAGGCAAATA TAAAGCCCTA
    GCAGATTGCA GGAAGAGAGG CTCAGAAGAC CTCCTGATTA GAAATGGAGA TGTCATTCAG
    CTTCTCCATG AGGATGCTGA GGGTCGATGG TTGGTGAAAA ATCTAAACAG AAGAAAAGAA
    GGTCTGATTC CAGGGAACAG TTTGCAGATT TTAATCGGTG ACTATAGGTT TCGGAATGCC
    AAAGATGCTG CTCTGAGTAA CAAGGAAACT AAGTTCCCCT TGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89235
    Clone ID Related Accession (Same CDS sequence) XM_015461453.1 , XM_015470208.1
    Accession Version XM_015461453.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGTGGGAGT TGGATATTCT
    TTCTTTCAAG CATGTAAACT TTTTTCAAAA GGGAAGAAGA CTTGGAGACA AAATCAGAGC
    AACACAAAAA CTGAAGCAGT GTATGATTGT CAGGAGAAGA GAAGTTCTGC TCAGTCCTCC
    AATTTGGACA ATGACAATAG CTTGGATATT TTAAAGAACC ACGTCCTAAA TGAGCTGATA
    CAGACGGAGA GGGCATATGT TAGGGAACTG TTTACTGTTT TGTTGGGCTA TAGAGCGGAG
    ATGGATAATC CAGAGATGTT TGTTCTTATG CCACCTCTCC TGAGAAGTAA AAGGGATGTT
    CTCTTTGGAA ACATGGCAGA AATATATGAA TTCCATAACA ACATTTTCAT GAGCAGTCTG
    GAAAATTGTG TTGATGCTCC AGAAAGAGTG GGATCTTGTT TCCTGGAAAG GAAAGATGAT
    TTTCAAATGT ATGCGAAGTA TTGTCAGAAT AAGCCCAGGT CAGAGGCAAT TTGGAAGAAG
    TATTCAGAAT GCGCCTTTTT CCAGGAATGT CAAAGAAAAT TGAAACACAG ACTTGGTCTG
    GATTCCTATT TACTGAAACC AGTGCAACGT ATCACTAAAT ACCAGTTACT GTTGAAGGAG
    CTATTAAAAT ATAGCAAAGA CTGTGTCGGA TCTGTAGAAT TAAAGGAGGC ACTTGACACA
    ATGCTGGATT TACTGAAGTC AGTTAATGAC TTAATGCATC AGACTGCAAT AAATGGCTAT
    ATCGGAAACT TAAATGAGCT GGGCAAGATG ATAATGCAGG GTGCGTTCAG TGTTTGGATT
    GGACACAAGA AAGGTACTAC AAAGATGAAG GATTTTGCTA GATTCAAACC AATGCAGCGG
    CACCTTTTCC TGTATGAAAA AGCCGTCGTA TTTTGTAAAA GGCGTGTTGA AAGTGGAGAA
    GGTGGTGATC GATACCCATC ATACAGTTTT AAGCACTGTT TGAAAATGGA TGAACTTGGA
    ATCACTGAAT ATGTAAAAGG AGATAACCGC AAGTTTGAAA TCTGGAATGC TGGAAAGGAA
    GAAGTTTATA TTGTCCAGGC TTCAAATATA GATATGAAGA TGATGTGGTT AAAAGAAATC
    AGAACTATTT TGTTGAAGCA ACAGGAACTT TTGACAGTTA AGAACCGAGA ACAGCATAAT
    CAGTTAACAG AGCAGAATCA TTTTTCTTCT CAGCAGAATG ACGAAGGGCA ACACAGAGGT
    TTTATAGGCG CTGAGGAACC TGAGTTGGGA CATATCAGCA CTATGGTGGA GGTCGGTGAG
    TCCATCACAT CAGTTCAAGC AGAAGCAAAT ACAGTTTGGA CTGAGATGTC ATCACCTGCA
    AAAGTCTCTG ATGAACCTGA GAAATGGTCC AGTAACTATT TCTACTCCTC TTATGATGGC
    CATGAAGAAG AAAGACCTCT AATGTCTCCA GGCAAATATA AAGCCCTAGC AGATTGCAGG
    AAGAGAGGCT CAGAAGACCT CCTGATTAGA AATGGAGATG TCATTCAGCT TCTCCATGAG
    GATGCTGAGG GTCGATGGTT GGTGAAAAAT CTAAACAGAA GAAAAGAAGG TCTGATTCCA
    GGGAACAGTT TGCAGATTTT AATCGGTGAC TATAGGTTTC GGAATGCCAA AGATGCTGCT
    CTGAGTAACA AGGAAACTAA GTTCCCCTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316939.1
    CDS317..3352
    Translation

    Target ORF information:

    RefSeq Version XM_015461453.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGTGGGAGT TGGATATTCT
    TTCTTTCAAG CATGTAAACT TTTTTCAAAA GGGAAGAAGA CTTGGAGACA AAATCAGAGC
    AACACAAAAA CTGAAGCAGT GTATGATTGT CAGGAGAAGA GAAGTTCTGC TCAGTCCTCC
    AATTTGGACA ATGACAATAG CTTGGATATT TTAAAGAACC ACGTCCTAAA TGAGCTGATA
    CAGACGGAGA GGGCATATGT TAGGGAACTG TTTACTGTTT TGTTGGGCTA TAGAGCGGAG
    ATGGATAATC CAGAGATGTT TGTTCTTATG CCACCTCTCC TGAGAAGTAA AAGGGATGTT
    CTCTTTGGAA ACATGGCAGA AATATATGAA TTCCATAACA ACATTTTCAT GAGCAGTCTG
    GAAAATTGTG TTGATGCTCC AGAAAGAGTG GGATCTTGTT TCCTGGAAAG GAAAGATGAT
    TTTCAAATGT ATGCGAAGTA TTGTCAGAAT AAGCCCAGGT CAGAGGCAAT TTGGAAGAAG
    TATTCAGAAT GCGCCTTTTT CCAGGAATGT CAAAGAAAAT TGAAACACAG ACTTGGTCTG
    GATTCCTATT TACTGAAACC AGTGCAACGT ATCACTAAAT ACCAGTTACT GTTGAAGGAG
    CTATTAAAAT ATAGCAAAGA CTGTGTCGGA TCTGTAGAAT TAAAGGAGGC ACTTGACACA
    ATGCTGGATT TACTGAAGTC AGTTAATGAC TTAATGCATC AGACTGCAAT AAATGGCTAT
    ATCGGAAACT TAAATGAGCT GGGCAAGATG ATAATGCAGG GTGCGTTCAG TGTTTGGATT
    GGACACAAGA AAGGTACTAC AAAGATGAAG GATTTTGCTA GATTCAAACC AATGCAGCGG
    CACCTTTTCC TGTATGAAAA AGCCGTCGTA TTTTGTAAAA GGCGTGTTGA AAGTGGAGAA
    GGTGGTGATC GATACCCATC ATACAGTTTT AAGCACTGTT TGAAAATGGA TGAACTTGGA
    ATCACTGAAT ATGTAAAAGG AGATAACCGC AAGTTTGAAA TCTGGAATGC TGGAAAGGAA
    GAAGTTTATA TTGTCCAGGC TTCAAATATA GATATGAAGA TGATGTGGTT AAAAGAAATC
    AGAACTATTT TGTTGAAGCA ACAGGAACTT TTGACAGTTA AGAACCGAGA ACAGCATAAT
    CAGTTAACAG AGCAGAATCA TTTTTCTTCT CAGCAGAATG ACGAAGGGCA ACACAGAGGT
    TTTATAGGCG CTGAGGAACC TGAGTTGGGA CATATCAGCA CTATGGTGGA GGTCGGTGAG
    TCCATCACAT CAGTTCAAGC AGAAGCAAAT ACAGTTTGGA CTGAGATGTC ATCACCTGCA
    AAAGTCTCTG ATGAACCTGA GAAATGGTCC AGTAACTATT TCTACTCCTC TTATGATGGC
    CATGAAGAAG AAAGACCTCT AATGTCTCCA GGCAAATATA AAGCCCTAGC AGATTGCAGG
    AAGAGAGGCT CAGAAGACCT CCTGATTAGA AATGGAGATG TCATTCAGCT TCTCCATGAG
    GATGCTGAGG GTCGATGGTT GGTGAAAAAT CTAAACAGAA GAAAAGAAGG TCTGATTCCA
    GGGAACAGTT TGCAGATTTT AATCGGTGAC TATAGGTTTC GGAATGCCAA AGATGCTGCT
    CTGAGTAACA AGGAAACTAA GTTCCCCTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89235
    Clone ID Related Accession (Same CDS sequence) XM_015461453.1 , XM_015470208.1
    Accession Version XM_015470208.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGTGGGAGT TGGATATTCT
    TTCTTTCAAG CATGTAAACT TTTTTCAAAA GGGAAGAAGA CTTGGAGACA AAATCAGAGC
    AACACAAAAA CTGAAGCAGT GTATGATTGT CAGGAGAAGA GAAGTTCTGC TCAGTCCTCC
    AATTTGGACA ATGACAATAG CTTGGATATT TTAAAGAACC ACGTCCTAAA TGAGCTGATA
    CAGACGGAGA GGGCATATGT TAGGGAACTG TTTACTGTTT TGTTGGGCTA TAGAGCGGAG
    ATGGATAATC CAGAGATGTT TGTTCTTATG CCACCTCTCC TGAGAAGTAA AAGGGATGTT
    CTCTTTGGAA ACATGGCAGA AATATATGAA TTCCATAACA ACATTTTCAT GAGCAGTCTG
    GAAAATTGTG TTGATGCTCC AGAAAGAGTG GGATCTTGTT TCCTGGAAAG GAAAGATGAT
    TTTCAAATGT ATGCGAAGTA TTGTCAGAAT AAGCCCAGGT CAGAGGCAAT TTGGAAGAAG
    TATTCAGAAT GCGCCTTTTT CCAGGAATGT CAAAGAAAAT TGAAACACAG ACTTGGTCTG
    GATTCCTATT TACTGAAACC AGTGCAACGT ATCACTAAAT ACCAGTTACT GTTGAAGGAG
    CTATTAAAAT ATAGCAAAGA CTGTGTCGGA TCTGTAGAAT TAAAGGAGGC ACTTGACACA
    ATGCTGGATT TACTGAAGTC AGTTAATGAC TTAATGCATC AGACTGCAAT AAATGGCTAT
    ATCGGAAACT TAAATGAGCT GGGCAAGATG ATAATGCAGG GTGCGTTCAG TGTTTGGATT
    GGACACAAGA AAGGTACTAC AAAGATGAAG GATTTTGCTA GATTCAAACC AATGCAGCGG
    CACCTTTTCC TGTATGAAAA AGCCGTCGTA TTTTGTAAAA GGCGTGTTGA AAGTGGAGAA
    GGTGGTGATC GATACCCATC ATACAGTTTT AAGCACTGTT TGAAAATGGA TGAACTTGGA
    ATCACTGAAT ATGTAAAAGG AGATAACCGC AAGTTTGAAA TCTGGAATGC TGGAAAGGAA
    GAAGTTTATA TTGTCCAGGC TTCAAATATA GATATGAAGA TGATGTGGTT AAAAGAAATC
    AGAACTATTT TGTTGAAGCA ACAGGAACTT TTGACAGTTA AGAACCGAGA ACAGCATAAT
    CAGTTAACAG AGCAGAATCA TTTTTCTTCT CAGCAGAATG ACGAAGGGCA ACACAGAGGT
    TTTATAGGCG CTGAGGAACC TGAGTTGGGA CATATCAGCA CTATGGTGGA GGTCGGTGAG
    TCCATCACAT CAGTTCAAGC AGAAGCAAAT ACAGTTTGGA CTGAGATGTC ATCACCTGCA
    AAAGTCTCTG ATGAACCTGA GAAATGGTCC AGTAACTATT TCTACTCCTC TTATGATGGC
    CATGAAGAAG AAAGACCTCT AATGTCTCCA GGCAAATATA AAGCCCTAGC AGATTGCAGG
    AAGAGAGGCT CAGAAGACCT CCTGATTAGA AATGGAGATG TCATTCAGCT TCTCCATGAG
    GATGCTGAGG GTCGATGGTT GGTGAAAAAT CTAAACAGAA GAAAAGAAGG TCTGATTCCA
    GGGAACAGTT TGCAGATTTT AATCGGTGAC TATAGGTTTC GGAATGCCAA AGATGCTGCT
    CTGAGTAACA AGGAAACTAA GTTCCCCTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325694.1
    CDS317..3352
    Translation

    Target ORF information:

    RefSeq Version XM_015470208.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470208.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAGAAG CAACTCCCCC GAGAGGCAAG ATGAGGTTCA GAAGGAATGC GTCTTCATTC 
    CCTGGGAACT TGCACTTGGT TTTGGTCCTT CGTCCCACCA GTTTTCTCCA ACGAACTTTC
    ACAGACATTG GATTTCGATT TAGTCAGGAA GATTTCATGC TCAAATTACC GGTTGTTATG
    CTGAGCTCAG TTAGTGATTT GCTGACATAC ATTGATGACA AGCAGTTAAC CACTGAGTTA
    GGCGGCACCT TGCAGTACAG CCACAGTGAA TGGATCACCT TCAGAAATGC TATAGAAAAT
    TTTGCCCTCA CAGTGAAAGA TACGGCTCAG ATGTTACAGT CCTTTGGAAC TGAACTGGCT
    GAGACAGAAC TACCAGGTGA CATTCCTGCA ATGGAAGAAA TTCTTGCAAT TCGTGCTGAA
    AGGTACCACC TGTTAAAGAA GGATATCACA GCTGTAACCA AAGAAGGAAA ATTTCTGCTA
    ACAAATCTGG AAGTGCCTGA TGCTGAGGGT GCTGTGAGTT CAAGACTTGA AAATGATCAG
    CCGACTAGTG GTGACTGGCA AACTATTAAT AAATTGCTGA CTCAAGTACA TGATATGGAA
    ATAGCTTTTG ATGGATTTTG GGAAAAACAC CAGCTAAAAA TGGAGCAGTA TTTGCAACTC
    TGGAAGTTTG AGCAGGATTT TCAAGAGCTC GTGAGTGAAG CTGAACTTAT ACTAAATCAA
    CAAGCGGAGT TGGCAGATGT AACAGGGACT ATAGCTCAAG TGAAACAAAA AATTAAAAAG
    TTGGAAAACT TGGATGAAAA TTCTCAGGAC CTGTTAGCAA AGGCCCGGTT CGTAATAATA
    CATGGACATA GGCTCGCAGC AAGTCATCAT TATGCCCTGG ATTTGATCTG TCAGAGGTGC
    AATGAACTAC GTTACCTTTC TGATATTTTG GTAAATGAAA TCAGAACCAA ACGGATACAG
    CTCAACAGGA CCTTTAAAAT GCATAAACTC CTACAGCAGG CTCTCCAGTG CTGTGATGAA
    GGAGAGTGCC TTCTGGCTAA TCAAGAAATA GACAAGTTTC AGTCTAAAGA AGATGCACAG
    AAGGCCCTCC AAGACATTGA AAATTTTCTT GAAATGGCTC TACCCTTTAT AAATTATGAT
    CCTGAAACCC TACAGTATGA ATTTGGTATA ATTTTATCTC CTGAACTCAA GGTTCAAATG
    CAGACCATCC AACTCAAGCT TGAAAACATC CGAAGTATAT TTGAGAACCA ACAGGCTGGT
    TTCAGAAACC TGACAGATAA ACATGTGAGG CCGATTCCGT TTGTAGTATC TGCTTCTGAC
    AATTTGGTCA GATCTGGAGC ACCATTTTTT TCACCTACAC AAGTGGGAGT TGGATATTCT
    TTCTTTCAAG CATGTAAACT TTTTTCAAAA GGGAAGAAGA CTTGGAGACA AAATCAGAGC
    AACACAAAAA CTGAAGCAGT GTATGATTGT CAGGAGAAGA GAAGTTCTGC TCAGTCCTCC
    AATTTGGACA ATGACAATAG CTTGGATATT TTAAAGAACC ACGTCCTAAA TGAGCTGATA
    CAGACGGAGA GGGCATATGT TAGGGAACTG TTTACTGTTT TGTTGGGCTA TAGAGCGGAG
    ATGGATAATC CAGAGATGTT TGTTCTTATG CCACCTCTCC TGAGAAGTAA AAGGGATGTT
    CTCTTTGGAA ACATGGCAGA AATATATGAA TTCCATAACA ACATTTTCAT GAGCAGTCTG
    GAAAATTGTG TTGATGCTCC AGAAAGAGTG GGATCTTGTT TCCTGGAAAG GAAAGATGAT
    TTTCAAATGT ATGCGAAGTA TTGTCAGAAT AAGCCCAGGT CAGAGGCAAT TTGGAAGAAG
    TATTCAGAAT GCGCCTTTTT CCAGGAATGT CAAAGAAAAT TGAAACACAG ACTTGGTCTG
    GATTCCTATT TACTGAAACC AGTGCAACGT ATCACTAAAT ACCAGTTACT GTTGAAGGAG
    CTATTAAAAT ATAGCAAAGA CTGTGTCGGA TCTGTAGAAT TAAAGGAGGC ACTTGACACA
    ATGCTGGATT TACTGAAGTC AGTTAATGAC TTAATGCATC AGACTGCAAT AAATGGCTAT
    ATCGGAAACT TAAATGAGCT GGGCAAGATG ATAATGCAGG GTGCGTTCAG TGTTTGGATT
    GGACACAAGA AAGGTACTAC AAAGATGAAG GATTTTGCTA GATTCAAACC AATGCAGCGG
    CACCTTTTCC TGTATGAAAA AGCCGTCGTA TTTTGTAAAA GGCGTGTTGA AAGTGGAGAA
    GGTGGTGATC GATACCCATC ATACAGTTTT AAGCACTGTT TGAAAATGGA TGAACTTGGA
    ATCACTGAAT ATGTAAAAGG AGATAACCGC AAGTTTGAAA TCTGGAATGC TGGAAAGGAA
    GAAGTTTATA TTGTCCAGGC TTCAAATATA GATATGAAGA TGATGTGGTT AAAAGAAATC
    AGAACTATTT TGTTGAAGCA ACAGGAACTT TTGACAGTTA AGAACCGAGA ACAGCATAAT
    CAGTTAACAG AGCAGAATCA TTTTTCTTCT CAGCAGAATG ACGAAGGGCA ACACAGAGGT
    TTTATAGGCG CTGAGGAACC TGAGTTGGGA CATATCAGCA CTATGGTGGA GGTCGGTGAG
    TCCATCACAT CAGTTCAAGC AGAAGCAAAT ACAGTTTGGA CTGAGATGTC ATCACCTGCA
    AAAGTCTCTG ATGAACCTGA GAAATGGTCC AGTAACTATT TCTACTCCTC TTATGATGGC
    CATGAAGAAG AAAGACCTCT AATGTCTCCA GGCAAATATA AAGCCCTAGC AGATTGCAGG
    AAGAGAGGCT CAGAAGACCT CCTGATTAGA AATGGAGATG TCATTCAGCT TCTCCATGAG
    GATGCTGAGG GTCGATGGTT GGTGAAAAAT CTAAACAGAA GAAAAGAAGG TCTGATTCCA
    GGGAACAGTT TGCAGATTTT AATCGGTGAC TATAGGTTTC GGAATGCCAA AGATGCTGCT
    CTGAGTAACA AGGAAACTAA GTTCCCCTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12869
    Clone ID Related Accession (Same CDS sequence) XM_005200582.3 , XM_005227557.3
    Accession Version XM_005200582.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGTCTCCAG GCAAATATAA AGCCCTAGCA
    GATTGCAGGA AGAGAGGCTC AGAAGACCTC CTGATTAGAA ATGGAGATGT CATTCAGCTT
    CTCCATGAGG ATGCTGAGGG TCGATGGTTG GTGAAAAATC TAAACAGAAG AAAAGAAGGT
    CTGATTCCAG GGAACAGTTT GCAGATTTTA ATCGGTGACT ATAGGTTTCG GAATGCCAAA
    GATGCTGCTC TGAGTAACAA GGAAACTAAG TTCCCCTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005200639.1
    CDS116..3460
    Translation

    Target ORF information:

    RefSeq Version XM_005200582.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005200582.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGTCTCCAG GCAAATATAA AGCCCTAGCA
    GATTGCAGGA AGAGAGGCTC AGAAGACCTC CTGATTAGAA ATGGAGATGT CATTCAGCTT
    CTCCATGAGG ATGCTGAGGG TCGATGGTTG GTGAAAAATC TAAACAGAAG AAAAGAAGGT
    CTGATTCCAG GGAACAGTTT GCAGATTTTA ATCGGTGACT ATAGGTTTCG GAATGCCAAA
    GATGCTGCTC TGAGTAACAA GGAAACTAAG TTCCCCTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12869
    Clone ID Related Accession (Same CDS sequence) XM_005200582.3 , XM_005227557.3
    Accession Version XM_005227557.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGTCTCCAG GCAAATATAA AGCCCTAGCA
    GATTGCAGGA AGAGAGGCTC AGAAGACCTC CTGATTAGAA ATGGAGATGT CATTCAGCTT
    CTCCATGAGG ATGCTGAGGG TCGATGGTTG GTGAAAAATC TAAACAGAAG AAAAGAAGGT
    CTGATTCCAG GGAACAGTTT GCAGATTTTA ATCGGTGACT ATAGGTTTCG GAATGCCAAA
    GATGCTGCTC TGAGTAACAA GGAAACTAAG TTCCCCTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227614.1
    CDS116..3460
    Translation

    Target ORF information:

    RefSeq Version XM_005227557.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227557.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGTCTCCAG GCAAATATAA AGCCCTAGCA
    GATTGCAGGA AGAGAGGCTC AGAAGACCTC CTGATTAGAA ATGGAGATGT CATTCAGCTT
    CTCCATGAGG ATGCTGAGGG TCGATGGTTG GTGAAAAATC TAAACAGAAG AAAAGAAGGT
    CTGATTCCAG GGAACAGTTT GCAGATTTTA ATCGGTGACT ATAGGTTTCG GAATGCCAAA
    GATGCTGCTC TGAGTAACAA GGAAACTAAG TTCCCCTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12870
    Clone ID Related Accession (Same CDS sequence) XM_005200583.3 , XM_005227558.3
    Accession Version XM_005200583.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGGCCCTGG GAACCCCCCA ACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005200640.1
    CDS114..3230
    Translation

    Target ORF information:

    RefSeq Version XM_005200583.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005200583.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGGCCCTGG GAACCCCCCA ACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12870
    Clone ID Related Accession (Same CDS sequence) XM_005200583.3 , XM_005227558.3
    Accession Version XM_005227558.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product proto-oncogene DBL isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104661.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGGCCCTGG GAACCCCCCA ACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005227615.1
    CDS114..3230
    Translation

    Target ORF information:

    RefSeq Version XM_005227558.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence (MCF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005227558.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCCATGG CTCTGCGGCG CGTGCGGATT TCAGATAAGT ACAGCAAGAG CTACTTCAGC 
    CTGCTGCAAG CAGCTTCCGT CGCAAAAGAC GAAGTGCTTC TCTGTGTAAA GGACGTCAGC
    CACTTCTTAA TGCAAGACAT TGCCTTCCTG TCTGGTGGCC GGGGAAAGGA TGATGCTTGG
    ATCATTACGT TTCCAGAAAA TTGTAATTTC AAATGCATAC CAGAAGAAGT TATAGCAAAA
    GTACTGACTT ACTTGACATC TGTTGCAAGG CAAAATGGAT CTGACTCCCG ATTTACCATT
    ATTCTTGATC GAAGATTGGA TACCTGGTCT TCTCTCAAAA TGTCTCTCCA AAAAATCTCA
    TCTTCATTCC CTGGGAACTT GCACTTGGTT TTGGTCCTTC GTCCCACCAG TTTTCTCCAA
    CGAACTTTCA CAGACATTGG ATTTCGATTT AGTCAGGAAG ATTTCATGCT CAAATTACCG
    GTTGTTATGC TGAGCTCAGT TAGTGATTTG CTGACATACA TTGATGACAA GCAGTTAACC
    ACTGAGTTAG GCGGCACCTT GCAGTACAGC CACAGTGAAT GGATCACCTT CAGAAATGCT
    ATAGAAAATT TTGCCCTCAC AGTGAAAGAT ACGGCTCAGA TGTTACAGTC CTTTGGAACT
    GAACTGGCTG AGACAGAACT ACCAGGTGAC ATTCCTGCAA TGGAAGAAAT TCTTGCAATT
    CGTGCTGAAA GGTACCACCT GTTAAAGAAG GATATCACAG CTGTAACCAA AGAAGGAAAA
    TTTCTGCTAA CAAATCTGGA AGTGCCTGAT GCTGAGGGTG CTGTGAGTTC AAGACTTGAA
    AATGATCAGC CGACTAGTGG TGACTGGCAA ACTATTAATA AATTGCTGAC TCAAGTACAT
    GATATGGAAA TAGCTTTTGA TGGATTTTGG GAAAAACACC AGCTAAAAAT GGAGCAGTAT
    TTGCAACTCT GGAAGTTTGA GCAGGATTTT CAAGAGCTCG TGAGTGAAGC TGAACTTATA
    CTAAATCAAC AAGCGGAGTT GGCAGATGTA ACAGGGACTA TAGCTCAAGT GAAACAAAAA
    ATTAAAAAGT TGGAAAACTT GGATGAAAAT TCTCAGGACC TGTTAGCAAA GGCCCGGTTC
    GTAATAATAC ATGGACATAG GCTCGCAGCA AGTCATCATT ATGCCCTGGA TTTGATCTGT
    CAGAGGTGCA ATGAACTACG TTACCTTTCT GATATTTTGG TAAATGAAAT CAGAACCAAA
    CGGATACAGC TCAACAGGAC CTTTAAAATG CATAAACTCC TACAGCAGGC TCTCCAGTGC
    TGTGATGAAG GAGAGTGCCT TCTGGCTAAT CAAGAAATAG ACAAGTTTCA GTCTAAAGAA
    GATGCACAGA AGGCCCTCCA AGACATTGAA AATTTTCTTG AAATGGCTCT ACCCTTTATA
    AATTATGATC CTGAAACCCT ACAGTATGAA TTTGGTATAA TTTTATCTCC TGAACTCAAG
    GTTCAAATGC AGACCATCCA ACTCAAGCTT GAAAACATCC GAAGTATATT TGAGAACCAA
    CAGGCTGGTT TCAGAAACCT GACAGATAAA CATGTGAGGC CGATTCCGTT TGTAGTATCT
    GCTTCTGACA ATTTGGTCAG ATCTGGAGCA CCATTTTTTT CACCTACACA AGTGGGAGTT
    GGATATTCTT TCTTTCAAGC ATGTAAACTT TTTTCAAAAG GGAAGAAGAC TTGGAGACAA
    AATCAGAGCA ACACAAAAAC TGAAGCAGTG TATGATTGTC AGGAGAAGAG AAGTTCTGCT
    CAGTCCTCCA ATTTGGACAA TGACAATAGC TTGGATATTT TAAAGAACCA CGTCCTAAAT
    GAGCTGATAC AGACGGAGAG GGCATATGTT AGGGAACTGT TTACTGTTTT GTTGGGCTAT
    AGAGCGGAGA TGGATAATCC AGAGATGTTT GTTCTTATGC CACCTCTCCT GAGAAGTAAA
    AGGGATGTTC TCTTTGGAAA CATGGCAGAA ATATATGAAT TCCATAACAA CATTTTCATG
    AGCAGTCTGG AAAATTGTGT TGATGCTCCA GAAAGAGTGG GATCTTGTTT CCTGGAAAGG
    AAAGATGATT TTCAAATGTA TGCGAAGTAT TGTCAGAATA AGCCCAGGTC AGAGGCAATT
    TGGAAGAAGT ATTCAGAATG CGCCTTTTTC CAGGAATGTC AAAGAAAATT GAAACACAGA
    CTTGGTCTGG ATTCCTATTT ACTGAAACCA GTGCAACGTA TCACTAAATA CCAGTTACTG
    TTGAAGGAGC TATTAAAATA TAGCAAAGAC TGTGTCGGAT CTGTAGAATT AAAGGAGGCA
    CTTGACACAA TGCTGGATTT ACTGAAGTCA GTTAATGACT TAATGCATCA GACTGCAATA
    AATGGCTATA TCGGAAACTT AAATGAGCTG GGCAAGATGA TAATGCAGGG TGCGTTCAGT
    GTTTGGATTG GACACAAGAA AGGTACTACA AAGATGAAGG ATTTTGCTAG ATTCAAACCA
    ATGCAGCGGC ACCTTTTCCT GTATGAAAAA GCCGTCGTAT TTTGTAAAAG GCGTGTTGAA
    AGTGGAGAAG GTGGTGATCG ATACCCATCA TACAGTTTTA AGCACTGTTT GAAAATGGAT
    GAACTTGGAA TCACTGAATA TGTAAAAGGA GATAACCGCA AGTTTGAAAT CTGGAATGCT
    GGAAAGGAAG AAGTTTATAT TGTCCAGGCT TCAAATATAG ATATGAAGAT GATGTGGTTA
    AAAGAAATCA GAACTATTTT GTTGAAGCAA CAGGAACTTT TGACAGTTAA GAACCGAGAA
    CAGCATAATC AGTTAACAGA GCAGAATCAT TTTTCTTCTC AGCAGAATGA CGAAGGGCAA
    CACAGAGGTT TTATAGGCGC TGAGGAACCT GAGTTGGGAC ATATCAGCAC TATGGTGGAG
    GTCGGTGAGT CCATCACATC AGTTCAAGCA GAAGCAAATA CAGTTTGGAC TGAGATGTCA
    TCACCTGCAA AAGTCTCTGA TGAACCTGAG AAATGGTCCA GTAACTATTT CTACTCCTCT
    TATGATGGCC ATGAAGAAGA AAGACCTCTA ATGGCCCTGG GAACCCCCCA ACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.