FAM135A cDNA ORF clone, Bos taurus(cattle)

The following FAM135A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAM135A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237926 XM_024996872.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237927 XM_024996873.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237928 XM_024996874.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237929 XM_024996875.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa22442 XM_015464712.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22442 XM_002690006.5
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06014 XM_002705105.4
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa06014 XM_010808337.2
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa06014 XM_015464709.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa06014 XM_005210636.4
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa06014 XM_024996871.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa06015 XM_005210639.4
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa06015 XM_015464711.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22441 XM_015464710.1
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa22441 XM_002690004.5
Latest version!
Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa06014 XM_005210636.3 Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa06015 XM_005210639.3 Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22442 XM_002690006.4 Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22441 XM_002690004.4 Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237926
    Clone ID Related Accession (Same CDS sequence) XM_024996872.1
    Accession Version XM_024996872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GGTGCGCAGG
    TTTTCTGAGG CCTTCTTTTG TTTTGAACAT CCAAGAGAAG CTGCCATTGC ATACCAGGAA
    CTTCATGCTC AGAGTCATCT CCAGATGTGC ACCGCTATCA AAAACACTTC CTTCTGCAGC
    TCTCTCCCAC CCCTGCCTAT TGAATGTAGT GAATTAGATG GAGATCTCAA TTCGTTACCT
    ATTATCTTTG AAGATAGGTA TTTAGATTCA GTTACTGAAG ACTTAGATGC ACCCTGGATG
    GGAATTCAGA GTCTTCAGAG ATCAGAGTCC AGTAGAATGG ATAAATGTGA GACTGAAGAA
    AGCACTCTAG CTGGACTTTC CAGCCCAGAG TTGAAAATCA GACCTGCTGG GACCTCCAGT
    TTTTGGTCTA CAGAAGGTGA AAAGCAGCTA ACAAAGTCTC TAAAAGGAAA GAATGAAGAA
    TCAAATAAAT CCAAGGTTAA GGTTACTAAG CTCATGAAAA CAATGAAACC TGAAAACACA
    AAAAAATTAA TAAAACAGAA CTCTAAGGAT TTGGTGGTTT TGGTAGGCTA CAAATGCTTG
    AAAAGTACAG CATCAAATGA TCTCTTTAAA AGCTTTGAAG GCAACCCTAC ACATAGTCAG
    AAGGAAGGTC TGGACCCCAC AATATATGGA TATAATTTTG ATCCAAAGAC CTACATGAGA
    CAGACAAGTC AAAAGGAAGC TAGCTATTTG CCAGCTAATA CAGAAAGAAC TGAACAAAAG
    TCTCCAGATA TTGAAAATGT GCAACCAGAC CTGTTTGATC CTTTGAACTC TGGCAGCCTA
    AATCTTTGTG CAAATTTGTC CATTTCAGGT AAACTTGATA TCTCCCAGGA TGATAGTGAA
    ATTGCACAAG TGGAACACAG TGTGGCATCC AGAAGCTCAT CAGACGATTG CCATGATCGT
    CAGTCTGCCC CCACTTCAGG AGTTAGGACA ATTGAAGTTA AGCCATGTAA TAAAGACTGT
    TTCAGTGGAG AGAAAGTAAC TGTTAAAATA GGACCTTGGA CAGAGCTTCA ACAAGATGAA
    ATATTTGTGG ATAATTTACA ACTGCCCAAT TTTGAGTCCT TAGACTCTAA TGGTAAATCT
    AAACCTATGG AAATAACACT TGAAAAGGAA GCTTTGCAGG AAGCTAAGTG TCATTCTGTC
    GGGGAATCGT TAGCTAAGTT AAGAAATAAT CAACCTGCTT CTACAAGAGA ATACCACCTT
    GTAGTGAGTG GAGACACCAT TAAGTTACCA GACATTAGTG CCACGTGTGC CTCATCTCGG
    TTCTCAGACT CAGGTGTGGA AAGTGAACCA AGCTCTGTTG CAGCACATCC AAACCCTGAC
    ATAGTCTTTG AAACTGTACC AGGGCAAGGT CTTTTCATCA GTGAAAGGTT ATTTCCTCAG
    CTCTTGATGA AGCCTGACTG TAATGTAAAG TTTTCACTAG GCAGTCACTG TACTGAGAGT
    ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC TTAACCTCCA TAAATTCTCT GCCTTCTGAT
    GACGAGCTGT CACCTGATGA AAATCCTAAG AAATCTGCTG TATCTGAATG CCACCTAAGT
    GATAGCAAAA CTGTGTTAAA TCTAGGAACA ACTGATGTGC CAAAATGTGA CGATAGGAAA
    AAGTCAAGTA TTATTTTGCA GCAGCAGAGT GTCATATTTT CAGGGCATTT GGACAATGAA
    ACTATAGAAA TACATTCCTT AAATTCAAGC ACTAAAGACC CTTTACAATT TGTTTTTTCA
    GATGAAGATA CTTCCAGTGA TGTGAAAAGT AGTTGTAGCT CCAAACCTAA CTTGGACACT
    GTGTGTAAAG ACTCCCAGAG TCCTGATAAA TCCAGTGACT CTGTAGGAAC CACAGTTCCA
    TTAAGTTCAG AACTGGCTTG TTCAGGCACC CCTTGTGTCG TTTCAGGTTC CGTTTCCACC
    AGGGCAGATA GCAGTGAAGA TAGAACTGTG AAAAGGAAAA ACAGCGATGC ATTAAATCCC
    AAACAAATGT GTTCAGAAGC CCCTGCAGTT AAAGATGAAA CTTACCTGGG TACAGGTGAT
    GCTTTTTCAG CCAGTCCTGA TATGGTAAAG CAAGGGCTTG TGGAAAATTA TTTTGGCTGT
    CAAAGCAGTA CGGATGTTTC TGACACATGT GCTATTAGCT ACAGCCATTC AGTTAGCCCT
    CAGAAAGAAA CTTCTGGAAA AGAAATGAGT AGTCCTCAGC GGGATCAGGG TAAAGATGAG
    GAAGAGGAAG AGCAGGATCA ACAAATGGTT CAAAACGGGT ATTATGAGGA GGCAGATTAC
    TCCTCTCTGG AGGCCGCGGG AGATGCTCAC TGTGCAGATG GAGCTGACCT GGGAGGAGAA
    AGACTTGCCA AGTCTGAAAG ACTGGCCGGC GACTATCTGA GCGATGGCAT AACCATGCCT
    ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC CCGTCTGCAC CACGAGAGTC CCCTTGTGGC
    GTTAAGTACT CTTCTAAGAG TAAGTGTGAC GCTGTTACCA AGCAGCCGAG CAGCGCCTCC
    CACAGCTGCA CGTCATCTCT GTCCTGGTAC GAAAACTCGC CAAAGCCTCA GATACAAGCC
    TTCCTTCAGG CAAAAGATGA ATTGAAGCAA CTTAAACTCC CTGGGTTCAT GTACAGCGAT
    GTTCCTCTGC TGGCATCCTC AGTCCCTTAT TTTTGCGTGG AGGAGGAGGA GGAGGACCAT
    TGTGGAAATG GAGTGCATCT CATTGTCTGT GTGCATGGCC TAGACGGTAA CAGTGCAGAT
    CTCCGATTAG TAAAAACTTA CATTGAACTT GGGCTTCCTG GGGAAAGAAT TGATTTTCTT
    ATGTCTGAAA GAAATCAGAA TGATACTTTT GCTGATTTTG ATAGCATGAC TGATCGCCTT
    TTGGATGAGA TAATACAATA TATTCAGATA TATAGTCTAA CAGTCTCAAA AATAAGCTTT
    ATTGGACATT CTTTGGGCAA TTTAATAATC CGTTCAGTGC TTACAAGACC ACGGTTTAAA
    TATTACCTCA GCAGGCTTCA TACCTTTCTT TCACTTTCTG GACCTCACCT TGGTACACTC
    TACAATAGCA GTGCTCTTGT TAATACAGGT CTGTGGTTTA TGCAGAAATG GAAAAAATCA
    GGTTCTCTTT TACAATTGAC ATGTCGTGAT CATTCAGACC CTCGCCAAAC TTTCTTATAC
    AAGCTTAGTA ATAAAGCAGG GCTTCACTAC TTCAGAAATG TTGTCCTAGT GGGATCTCTG
    CAAGATCGCT ATGTTCCTTA TCATTCTGCC CGCATTGAAA TGTGCAAGAC AGCTTTGAAG
    GACAAACAGT CAGGACAGAT CTATTCAGAA ATGATCCACA ACTTACTTCG CCCGGTTCTG
    CAAAGCAAGG ACTGTAACTT GGTTCGATAT AATGTCATCA ATGCGTTGCC CAATACAGCT
    GATTCACTCA TTGGCAGAGC TGCACATATA GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG
    AAATTCTTTC TGGTTGCTGC CCTCAAATAT TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852640.1
    CDS375..4853
    Translation

    Target ORF information:

    RefSeq Version XM_024996872.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GGTGCGCAGG
    TTTTCTGAGG CCTTCTTTTG TTTTGAACAT CCAAGAGAAG CTGCCATTGC ATACCAGGAA
    CTTCATGCTC AGAGTCATCT CCAGATGTGC ACCGCTATCA AAAACACTTC CTTCTGCAGC
    TCTCTCCCAC CCCTGCCTAT TGAATGTAGT GAATTAGATG GAGATCTCAA TTCGTTACCT
    ATTATCTTTG AAGATAGGTA TTTAGATTCA GTTACTGAAG ACTTAGATGC ACCCTGGATG
    GGAATTCAGA GTCTTCAGAG ATCAGAGTCC AGTAGAATGG ATAAATGTGA GACTGAAGAA
    AGCACTCTAG CTGGACTTTC CAGCCCAGAG TTGAAAATCA GACCTGCTGG GACCTCCAGT
    TTTTGGTCTA CAGAAGGTGA AAAGCAGCTA ACAAAGTCTC TAAAAGGAAA GAATGAAGAA
    TCAAATAAAT CCAAGGTTAA GGTTACTAAG CTCATGAAAA CAATGAAACC TGAAAACACA
    AAAAAATTAA TAAAACAGAA CTCTAAGGAT TTGGTGGTTT TGGTAGGCTA CAAATGCTTG
    AAAAGTACAG CATCAAATGA TCTCTTTAAA AGCTTTGAAG GCAACCCTAC ACATAGTCAG
    AAGGAAGGTC TGGACCCCAC AATATATGGA TATAATTTTG ATCCAAAGAC CTACATGAGA
    CAGACAAGTC AAAAGGAAGC TAGCTATTTG CCAGCTAATA CAGAAAGAAC TGAACAAAAG
    TCTCCAGATA TTGAAAATGT GCAACCAGAC CTGTTTGATC CTTTGAACTC TGGCAGCCTA
    AATCTTTGTG CAAATTTGTC CATTTCAGGT AAACTTGATA TCTCCCAGGA TGATAGTGAA
    ATTGCACAAG TGGAACACAG TGTGGCATCC AGAAGCTCAT CAGACGATTG CCATGATCGT
    CAGTCTGCCC CCACTTCAGG AGTTAGGACA ATTGAAGTTA AGCCATGTAA TAAAGACTGT
    TTCAGTGGAG AGAAAGTAAC TGTTAAAATA GGACCTTGGA CAGAGCTTCA ACAAGATGAA
    ATATTTGTGG ATAATTTACA ACTGCCCAAT TTTGAGTCCT TAGACTCTAA TGGTAAATCT
    AAACCTATGG AAATAACACT TGAAAAGGAA GCTTTGCAGG AAGCTAAGTG TCATTCTGTC
    GGGGAATCGT TAGCTAAGTT AAGAAATAAT CAACCTGCTT CTACAAGAGA ATACCACCTT
    GTAGTGAGTG GAGACACCAT TAAGTTACCA GACATTAGTG CCACGTGTGC CTCATCTCGG
    TTCTCAGACT CAGGTGTGGA AAGTGAACCA AGCTCTGTTG CAGCACATCC AAACCCTGAC
    ATAGTCTTTG AAACTGTACC AGGGCAAGGT CTTTTCATCA GTGAAAGGTT ATTTCCTCAG
    CTCTTGATGA AGCCTGACTG TAATGTAAAG TTTTCACTAG GCAGTCACTG TACTGAGAGT
    ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC TTAACCTCCA TAAATTCTCT GCCTTCTGAT
    GACGAGCTGT CACCTGATGA AAATCCTAAG AAATCTGCTG TATCTGAATG CCACCTAAGT
    GATAGCAAAA CTGTGTTAAA TCTAGGAACA ACTGATGTGC CAAAATGTGA CGATAGGAAA
    AAGTCAAGTA TTATTTTGCA GCAGCAGAGT GTCATATTTT CAGGGCATTT GGACAATGAA
    ACTATAGAAA TACATTCCTT AAATTCAAGC ACTAAAGACC CTTTACAATT TGTTTTTTCA
    GATGAAGATA CTTCCAGTGA TGTGAAAAGT AGTTGTAGCT CCAAACCTAA CTTGGACACT
    GTGTGTAAAG ACTCCCAGAG TCCTGATAAA TCCAGTGACT CTGTAGGAAC CACAGTTCCA
    TTAAGTTCAG AACTGGCTTG TTCAGGCACC CCTTGTGTCG TTTCAGGTTC CGTTTCCACC
    AGGGCAGATA GCAGTGAAGA TAGAACTGTG AAAAGGAAAA ACAGCGATGC ATTAAATCCC
    AAACAAATGT GTTCAGAAGC CCCTGCAGTT AAAGATGAAA CTTACCTGGG TACAGGTGAT
    GCTTTTTCAG CCAGTCCTGA TATGGTAAAG CAAGGGCTTG TGGAAAATTA TTTTGGCTGT
    CAAAGCAGTA CGGATGTTTC TGACACATGT GCTATTAGCT ACAGCCATTC AGTTAGCCCT
    CAGAAAGAAA CTTCTGGAAA AGAAATGAGT AGTCCTCAGC GGGATCAGGG TAAAGATGAG
    GAAGAGGAAG AGCAGGATCA ACAAATGGTT CAAAACGGGT ATTATGAGGA GGCAGATTAC
    TCCTCTCTGG AGGCCGCGGG AGATGCTCAC TGTGCAGATG GAGCTGACCT GGGAGGAGAA
    AGACTTGCCA AGTCTGAAAG ACTGGCCGGC GACTATCTGA GCGATGGCAT AACCATGCCT
    ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC CCGTCTGCAC CACGAGAGTC CCCTTGTGGC
    GTTAAGTACT CTTCTAAGAG TAAGTGTGAC GCTGTTACCA AGCAGCCGAG CAGCGCCTCC
    CACAGCTGCA CGTCATCTCT GTCCTGGTAC GAAAACTCGC CAAAGCCTCA GATACAAGCC
    TTCCTTCAGG CAAAAGATGA ATTGAAGCAA CTTAAACTCC CTGGGTTCAT GTACAGCGAT
    GTTCCTCTGC TGGCATCCTC AGTCCCTTAT TTTTGCGTGG AGGAGGAGGA GGAGGACCAT
    TGTGGAAATG GAGTGCATCT CATTGTCTGT GTGCATGGCC TAGACGGTAA CAGTGCAGAT
    CTCCGATTAG TAAAAACTTA CATTGAACTT GGGCTTCCTG GGGAAAGAAT TGATTTTCTT
    ATGTCTGAAA GAAATCAGAA TGATACTTTT GCTGATTTTG ATAGCATGAC TGATCGCCTT
    TTGGATGAGA TAATACAATA TATTCAGATA TATAGTCTAA CAGTCTCAAA AATAAGCTTT
    ATTGGACATT CTTTGGGCAA TTTAATAATC CGTTCAGTGC TTACAAGACC ACGGTTTAAA
    TATTACCTCA GCAGGCTTCA TACCTTTCTT TCACTTTCTG GACCTCACCT TGGTACACTC
    TACAATAGCA GTGCTCTTGT TAATACAGGT CTGTGGTTTA TGCAGAAATG GAAAAAATCA
    GGTTCTCTTT TACAATTGAC ATGTCGTGAT CATTCAGACC CTCGCCAAAC TTTCTTATAC
    AAGCTTAGTA ATAAAGCAGG GCTTCACTAC TTCAGAAATG TTGTCCTAGT GGGATCTCTG
    CAAGATCGCT ATGTTCCTTA TCATTCTGCC CGCATTGAAA TGTGCAAGAC AGCTTTGAAG
    GACAAACAGT CAGGACAGAT CTATTCAGAA ATGATCCACA ACTTACTTCG CCCGGTTCTG
    CAAAGCAAGG ACTGTAACTT GGTTCGATAT AATGTCATCA ATGCGTTGCC CAATACAGCT
    GATTCACTCA TTGGCAGAGC TGCACATATA GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG
    AAATTCTTTC TGGTTGCTGC CCTCAAATAT TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237927
    Clone ID Related Accession (Same CDS sequence) XM_024996873.1
    Accession Version XM_024996873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTAG CCTTTCCAGC TTCAGTTCAT GATTCTCTGA TTTGCAGTAA AACATTTCAA 
    ATTCTATATA AAAATGAGGA GGTTGTTTTA AATGATGTCA TGATCTTCAA AGTTAAAATG
    TTGTTGGATG AAAAAAAGAT TGAAGAAACC CTTGAGGAAA TGAATTTTCT GTTATCCCTG
    GATCTACACT TCACAGATGG AGATTATTCG GCAGATGATC TGAATGCATT GCAGCTAATA
    AGCAGCCGCA CACTGAAGCT GCACTTCAGC CTCCAGAGAG GCCTCCATCA CCACGTTAAC
    GTCATGTTTG ATTATTTCCA CCTTTCTGTT GTGTCTGTTA CAGTCCATGC GTCACTGGTT
    GCGCTACACC AGCCACTGAT AAGTTTTCCT CGTCCTGTGA AGACAACTTG GTTAAACAGA
    AATGCACCAG CACAAAACAA AGATTCTGTG ATTCCTACTC TTGAAAGTGT GGTCTTTGGT
    ATTAACTACA CAAAGCAGTT ATCACCAGAC GGCTGCAGCT TTGTCATCGC AGATTCCTTC
    CTGCGTCACG CCTACCGTTT CCACTACACC CTCTGCGCTG CCCTGCTGCT GGCCTTCAAG
    GGGCTGCACA GCTACTTCAT TATGGTAACA GAAGAGCTCC CCTCTTGTCA GAAACTAGAA
    CTGGCCAAAG CCAACATGCA GGTCCTTTAT GAGCGCCTTC TCAGAAGAAA ACAGCCAGCA
    ACCCAGAAAG ACACCTGTCT AGAGGAAATG GATGTAGAAG CTCGACTTAC TGAATTATGT
    GAAGAAGTTA AGAAAATAGA AAATCCTGAT GAACTGGCAG AACTTATAAA TATGAATCTT
    GCACAACTTT GCTCACTTTT AATGGCTTTA TGGGGACAAT TTCTGGAAGT TATAACACTA
    CATGAGGAAC TAAGACTATT ACTAGCACAA GAGCACCATA CTCTGAGGGT GCGCAGGTTT
    TCTGAGGCCT TCTTTTGTTT TGAACATCCA AGAGAAGCTG CCATTGCATA CCAGGAACTT
    CATGCTCAGA GTCATCTCCA GATGTGCACC GCTATCAAAA ACACTTCCTT CTGCAGCTCT
    CTCCCACCCC TGCCTATTGA ATGTAGTGAA TTAGATGGAG ATCTCAATTC GTTACCTATT
    ATCTTTGAAG ATAGGTATTT AGATTCAGTT ACTGAAGACT TAGATGCACC CTGGATGGGA
    ATTCAGAGTC TTCAGAGATC AGAGTCCAGT AGAATGGATA AATGTGAGAC TGAAGAAAGC
    ACTCTAGCTG GACTTTCCAG CCCAGAGTTG AAAATCAGAC CTGCTGGGAC CTCCAGTTTT
    TGGTCTACAG AAGGTGAAAA GCAGCTAACA AAGTCTCTAA AAGGAAAGAA TGAAGAATCA
    AATAAATCCA AGGTTAAGGT TACTAAGCTC ATGAAAACAA TGAAACCTGA AAACACAAAA
    AAATTAATAA AACAGAACTC TAAGGATTTG GTGGTTTTGG TAGGCTACAA ATGCTTGAAA
    AGTACAGCAT CAAATGATCT CTTTAAAAGC TTTGAAGGCA ACCCTACACA TAGTCAGAAG
    GAAGGTCTGG ACCCCACAAT ATATGGATAT AATTTTGATC CAAAGACCTA CATGAGACAG
    ACAAGTCAAA AGGAAGCTAG CTATTTGCCA GCTAATACAG AAAGAACTGA ACAAAAGTCT
    CCAGATATTG AAAATGTGCA ACCAGACCTG TTTGATCCTT TGAACTCTGG CAGCCTAAAT
    CTTTGTGCAA ATTTGTCCAT TTCAGGTAAA CTTGATATCT CCCAGGATGA TAGTGAAATT
    GCACAAGTGG AACACAGTGT GGCATCCAGA AGCTCATCAG ACGATTGCCA TGATCGTCAG
    TCTGCCCCCA CTTCAGGAGT TAGGACAATT GAAGTTAAGC CATGTAATAA AGACTGTTTC
    AGTGGAGAGA AAGTAACTGT TAAAATAGGA CCTTGGACAG AGCTTCAACA AGATGAAATA
    TTTGTGGATA ATTTACAACT GCCCAATTTT GAGTCCTTAG ACTCTAATGG TAAATCTAAA
    CCTATGGAAA TAACACTTGA AAAGGAAGCT TTGCAGGAAG CTAAGTGTCA TTCTGTCGGG
    GAATCGTTAG CTAAGTTAAG AAATAATCAA CCTGCTTCTA CAAGAGAATA CCACCTTGTA
    GTGAGTGGAG ACACCATTAA GTTACCAGAC ATTAGTGCCA CGTGTGCCTC ATCTCGGTTC
    TCAGACTCAG GTGTGGAAAG TGAACCAAGC TCTGTTGCAG CACATCCAAA CCCTGACATA
    GTCTTTGAAA CTGTACCAGG GCAAGGTCTT TTCATCAGTG AAAGGTTATT TCCTCAGCTC
    TTGATGAAGC CTGACTGTAA TGTAAAGTTT TCACTAGGCA GTCACTGTAC TGAGAGTACA
    AGTGCTTTCA GTGAGATCCA GTCGTCCTTA ACCTCCATAA ATTCTCTGCC TTCTGATGAC
    GAGCTGTCAC CTGATGAAAA TCCTAAGAAA TCTGCTGTAT CTGAATGCCA CCTAAGTGAT
    AGCAAAACTG TGTTAAATCT AGGAACAACT GATGTGCCAA AATGTGACGA TAGGAAAAAG
    TCAAGTATTA TTTTGCAGCA GCAGAGTGTC ATATTTTCAG GGCATTTGGA CAATGAAACT
    ATAGAAATAC ATTCCTTAAA TTCAAGCACT AAAGACCCTT TACAATTTGT TTTTTCAGAT
    GAAGATACTT CCAGTGATGT GAAAAGTAGT TGTAGCTCCA AACCTAACTT GGACACTGTG
    TGTAAAGACT CCCAGAGTCC TGATAAATCC AGTGACTCTG TAGGAACCAC AGTTCCATTA
    AGTTCAGAAC TGGCTTGTTC AGGCACCCCT TGTGTCGTTT CAGGTTCCGT TTCCACCAGG
    GCAGATAGCA GTGAAGATAG AACTGTGAAA AGGAAAAACA GCGATGCATT AAATCCCAAA
    CAAATGTGTT CAGAAGCCCC TGCAGTTAAA GATGAAACTT ACCTGGGTAC AGGTGATGCT
    TTTTCAGCCA GTCCTGATAT GGTAAAGCAA GGGCTTGTGG AAAATTATTT TGGCTGTCAA
    AGCAGTACGG ATGTTTCTGA CACATGTGCT ATTAGCTACA GCCATTCAGT TAGCCCTCAG
    AAAGAAACTT CTGGAAAAGA AATGAGTAGT CCTCAGCGGG ATCAGGGTAA AGATGAGGAA
    GAGGAAGAGC AGGATCAACA AATGGTTCAA AACGGGTATT ATGAGGAGGC AGATTACTCC
    TCTCTGGAGG CCGCGGGAGA TGCTCACTGT GCAGATGGAG CTGACCTGGG AGGAGAAAGA
    CTTGCCAAGT CTGAAAGACT GGCCGGCGAC TATCTGAGCG ATGGCATAAC CATGCCTACT
    GTCTGTACTT CTGGCTGTCT GTCCTTCCCG TCTGCACCAC GAGAGTCCCC TTGTGGCGTT
    AAGTACTCTT CTAAGAGTAA GTGTGACGCT GTTACCAAGC AGCCGAGCAG CGCCTCCCAC
    AGCTGCACGT CATCTCTGTC CTGGTACGAA AACTCGCCAA AGCCTCAGAT ACAAGCCTTC
    CTTCAGGCAA AAGATGAATT GAAGCAACTT AAACTCCCTG GGTTCATGTA CAGCGATGTT
    CCTCTGCTGG CATCCTCAGT CCCTTATTTT TGCGTGGAGG AGGAGGAGGA GGACCATTGT
    GGAAATGGAG TGCATCTCAT TGTCTGTGTG CATGGCCTAG ACGGTAACAG TGCAGATCTC
    CGATTAGTAA AAACTTACAT TGAACTTGGG CTTCCTGGGG AAAGAATTGA TTTTCTTATG
    TCTGAAAGAA ATCAGAATGA TACTTTTGCT GATTTTGATA GCATGACTGA TCGCCTTTTG
    GATGAGATAA TACAATATAT TCAGATATAT AGTCTAACAG TCTCAAAAAT AAGCTTTATT
    GGACATTCTT TGGGCAATTT AATAATCCGT TCAGTGCTTA CAAGACCACG GTTTAAATAT
    TACCTCAGCA GGCTTCATAC CTTTCTTTCA CTTTCTGGAC CTCACCTTGG TACACTCTAC
    AATAGCAGTG CTCTTGTTAA TACAGGTCTG TGGTTTATGC AGAAATGGAA AAAATCAGGT
    TCTCTTTTAC AATTGACATG TCGTGATCAT TCAGACCCTC GCCAAACTTT CTTATACAAG
    CTTAGTAATA AAGCAGGGCT TCACTACTTC AGAAATGTTG TCCTAGTGGG ATCTCTGCAA
    GATCGCTATG TTCCTTATCA TTCTGCCCGC ATTGAAATGT GCAAGACAGC TTTGAAGGAC
    AAACAGTCAG GACAGATCTA TTCAGAAATG ATCCACAACT TACTTCGCCC GGTTCTGCAA
    AGCAAGGACT GTAACTTGGT TCGATATAAT GTCATCAATG CGTTGCCCAA TACAGCTGAT
    TCACTCATTG GCAGAGCTGC ACATATAGCT GTTCTGGATT CAGAAATATT TTTAGAGAAA
    TTCTTTCTGG TTGCTGCCCT CAAATATTTC CAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852641.1
    CDS189..4664
    Translation

    Target ORF information:

    RefSeq Version XM_024996873.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACTTTAG CCTTTCCAGC TTCAGTTCAT GATTCTCTGA TTTGCAGTAA AACATTTCAA 
    ATTCTATATA AAAATGAGGA GGTTGTTTTA AATGATGTCA TGATCTTCAA AGTTAAAATG
    TTGTTGGATG AAAAAAAGAT TGAAGAAACC CTTGAGGAAA TGAATTTTCT GTTATCCCTG
    GATCTACACT TCACAGATGG AGATTATTCG GCAGATGATC TGAATGCATT GCAGCTAATA
    AGCAGCCGCA CACTGAAGCT GCACTTCAGC CTCCAGAGAG GCCTCCATCA CCACGTTAAC
    GTCATGTTTG ATTATTTCCA CCTTTCTGTT GTGTCTGTTA CAGTCCATGC GTCACTGGTT
    GCGCTACACC AGCCACTGAT AAGTTTTCCT CGTCCTGTGA AGACAACTTG GTTAAACAGA
    AATGCACCAG CACAAAACAA AGATTCTGTG ATTCCTACTC TTGAAAGTGT GGTCTTTGGT
    ATTAACTACA CAAAGCAGTT ATCACCAGAC GGCTGCAGCT TTGTCATCGC AGATTCCTTC
    CTGCGTCACG CCTACCGTTT CCACTACACC CTCTGCGCTG CCCTGCTGCT GGCCTTCAAG
    GGGCTGCACA GCTACTTCAT TATGGTAACA GAAGAGCTCC CCTCTTGTCA GAAACTAGAA
    CTGGCCAAAG CCAACATGCA GGTCCTTTAT GAGCGCCTTC TCAGAAGAAA ACAGCCAGCA
    ACCCAGAAAG ACACCTGTCT AGAGGAAATG GATGTAGAAG CTCGACTTAC TGAATTATGT
    GAAGAAGTTA AGAAAATAGA AAATCCTGAT GAACTGGCAG AACTTATAAA TATGAATCTT
    GCACAACTTT GCTCACTTTT AATGGCTTTA TGGGGACAAT TTCTGGAAGT TATAACACTA
    CATGAGGAAC TAAGACTATT ACTAGCACAA GAGCACCATA CTCTGAGGGT GCGCAGGTTT
    TCTGAGGCCT TCTTTTGTTT TGAACATCCA AGAGAAGCTG CCATTGCATA CCAGGAACTT
    CATGCTCAGA GTCATCTCCA GATGTGCACC GCTATCAAAA ACACTTCCTT CTGCAGCTCT
    CTCCCACCCC TGCCTATTGA ATGTAGTGAA TTAGATGGAG ATCTCAATTC GTTACCTATT
    ATCTTTGAAG ATAGGTATTT AGATTCAGTT ACTGAAGACT TAGATGCACC CTGGATGGGA
    ATTCAGAGTC TTCAGAGATC AGAGTCCAGT AGAATGGATA AATGTGAGAC TGAAGAAAGC
    ACTCTAGCTG GACTTTCCAG CCCAGAGTTG AAAATCAGAC CTGCTGGGAC CTCCAGTTTT
    TGGTCTACAG AAGGTGAAAA GCAGCTAACA AAGTCTCTAA AAGGAAAGAA TGAAGAATCA
    AATAAATCCA AGGTTAAGGT TACTAAGCTC ATGAAAACAA TGAAACCTGA AAACACAAAA
    AAATTAATAA AACAGAACTC TAAGGATTTG GTGGTTTTGG TAGGCTACAA ATGCTTGAAA
    AGTACAGCAT CAAATGATCT CTTTAAAAGC TTTGAAGGCA ACCCTACACA TAGTCAGAAG
    GAAGGTCTGG ACCCCACAAT ATATGGATAT AATTTTGATC CAAAGACCTA CATGAGACAG
    ACAAGTCAAA AGGAAGCTAG CTATTTGCCA GCTAATACAG AAAGAACTGA ACAAAAGTCT
    CCAGATATTG AAAATGTGCA ACCAGACCTG TTTGATCCTT TGAACTCTGG CAGCCTAAAT
    CTTTGTGCAA ATTTGTCCAT TTCAGGTAAA CTTGATATCT CCCAGGATGA TAGTGAAATT
    GCACAAGTGG AACACAGTGT GGCATCCAGA AGCTCATCAG ACGATTGCCA TGATCGTCAG
    TCTGCCCCCA CTTCAGGAGT TAGGACAATT GAAGTTAAGC CATGTAATAA AGACTGTTTC
    AGTGGAGAGA AAGTAACTGT TAAAATAGGA CCTTGGACAG AGCTTCAACA AGATGAAATA
    TTTGTGGATA ATTTACAACT GCCCAATTTT GAGTCCTTAG ACTCTAATGG TAAATCTAAA
    CCTATGGAAA TAACACTTGA AAAGGAAGCT TTGCAGGAAG CTAAGTGTCA TTCTGTCGGG
    GAATCGTTAG CTAAGTTAAG AAATAATCAA CCTGCTTCTA CAAGAGAATA CCACCTTGTA
    GTGAGTGGAG ACACCATTAA GTTACCAGAC ATTAGTGCCA CGTGTGCCTC ATCTCGGTTC
    TCAGACTCAG GTGTGGAAAG TGAACCAAGC TCTGTTGCAG CACATCCAAA CCCTGACATA
    GTCTTTGAAA CTGTACCAGG GCAAGGTCTT TTCATCAGTG AAAGGTTATT TCCTCAGCTC
    TTGATGAAGC CTGACTGTAA TGTAAAGTTT TCACTAGGCA GTCACTGTAC TGAGAGTACA
    AGTGCTTTCA GTGAGATCCA GTCGTCCTTA ACCTCCATAA ATTCTCTGCC TTCTGATGAC
    GAGCTGTCAC CTGATGAAAA TCCTAAGAAA TCTGCTGTAT CTGAATGCCA CCTAAGTGAT
    AGCAAAACTG TGTTAAATCT AGGAACAACT GATGTGCCAA AATGTGACGA TAGGAAAAAG
    TCAAGTATTA TTTTGCAGCA GCAGAGTGTC ATATTTTCAG GGCATTTGGA CAATGAAACT
    ATAGAAATAC ATTCCTTAAA TTCAAGCACT AAAGACCCTT TACAATTTGT TTTTTCAGAT
    GAAGATACTT CCAGTGATGT GAAAAGTAGT TGTAGCTCCA AACCTAACTT GGACACTGTG
    TGTAAAGACT CCCAGAGTCC TGATAAATCC AGTGACTCTG TAGGAACCAC AGTTCCATTA
    AGTTCAGAAC TGGCTTGTTC AGGCACCCCT TGTGTCGTTT CAGGTTCCGT TTCCACCAGG
    GCAGATAGCA GTGAAGATAG AACTGTGAAA AGGAAAAACA GCGATGCATT AAATCCCAAA
    CAAATGTGTT CAGAAGCCCC TGCAGTTAAA GATGAAACTT ACCTGGGTAC AGGTGATGCT
    TTTTCAGCCA GTCCTGATAT GGTAAAGCAA GGGCTTGTGG AAAATTATTT TGGCTGTCAA
    AGCAGTACGG ATGTTTCTGA CACATGTGCT ATTAGCTACA GCCATTCAGT TAGCCCTCAG
    AAAGAAACTT CTGGAAAAGA AATGAGTAGT CCTCAGCGGG ATCAGGGTAA AGATGAGGAA
    GAGGAAGAGC AGGATCAACA AATGGTTCAA AACGGGTATT ATGAGGAGGC AGATTACTCC
    TCTCTGGAGG CCGCGGGAGA TGCTCACTGT GCAGATGGAG CTGACCTGGG AGGAGAAAGA
    CTTGCCAAGT CTGAAAGACT GGCCGGCGAC TATCTGAGCG ATGGCATAAC CATGCCTACT
    GTCTGTACTT CTGGCTGTCT GTCCTTCCCG TCTGCACCAC GAGAGTCCCC TTGTGGCGTT
    AAGTACTCTT CTAAGAGTAA GTGTGACGCT GTTACCAAGC AGCCGAGCAG CGCCTCCCAC
    AGCTGCACGT CATCTCTGTC CTGGTACGAA AACTCGCCAA AGCCTCAGAT ACAAGCCTTC
    CTTCAGGCAA AAGATGAATT GAAGCAACTT AAACTCCCTG GGTTCATGTA CAGCGATGTT
    CCTCTGCTGG CATCCTCAGT CCCTTATTTT TGCGTGGAGG AGGAGGAGGA GGACCATTGT
    GGAAATGGAG TGCATCTCAT TGTCTGTGTG CATGGCCTAG ACGGTAACAG TGCAGATCTC
    CGATTAGTAA AAACTTACAT TGAACTTGGG CTTCCTGGGG AAAGAATTGA TTTTCTTATG
    TCTGAAAGAA ATCAGAATGA TACTTTTGCT GATTTTGATA GCATGACTGA TCGCCTTTTG
    GATGAGATAA TACAATATAT TCAGATATAT AGTCTAACAG TCTCAAAAAT AAGCTTTATT
    GGACATTCTT TGGGCAATTT AATAATCCGT TCAGTGCTTA CAAGACCACG GTTTAAATAT
    TACCTCAGCA GGCTTCATAC CTTTCTTTCA CTTTCTGGAC CTCACCTTGG TACACTCTAC
    AATAGCAGTG CTCTTGTTAA TACAGGTCTG TGGTTTATGC AGAAATGGAA AAAATCAGGT
    TCTCTTTTAC AATTGACATG TCGTGATCAT TCAGACCCTC GCCAAACTTT CTTATACAAG
    CTTAGTAATA AAGCAGGGCT TCACTACTTC AGAAATGTTG TCCTAGTGGG ATCTCTGCAA
    GATCGCTATG TTCCTTATCA TTCTGCCCGC ATTGAAATGT GCAAGACAGC TTTGAAGGAC
    AAACAGTCAG GACAGATCTA TTCAGAAATG ATCCACAACT TACTTCGCCC GGTTCTGCAA
    AGCAAGGACT GTAACTTGGT TCGATATAAT GTCATCAATG CGTTGCCCAA TACAGCTGAT
    TCACTCATTG GCAGAGCTGC ACATATAGCT GTTCTGGATT CAGAAATATT TTTAGAGAAA
    TTCTTTCTGG TTGCTGCCCT CAAATATTTC CAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237928
    Clone ID Related Accession (Same CDS sequence) XM_024996874.1
    Accession Version XM_024996874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCAGCAGA TTGAAGAAAC CCTTGAGGAA ATGAATTTTC TGTTATCCCT GGATCTACAC 
    TTCACAGATG GAGATTATTC GGCAGATGAT CTGAATGCAT TGCAGCTAAT AAGCAGCCGC
    ACACTGAAGC TGCACTTCAG CCTCCAGAGA GGCCTCCATC ACCACGTTAA CGTCATGTTT
    GATTATTTCC ACCTTTCTGT TGTGTCTGTT ACAGTCCATG CGTCACTGGT TGCGCTACAC
    CAGCCACTGA TAAGTTTTCC TCGTCCTGTG AAGACAACTT GGTTAAACAG AAATGCACCA
    GCACAAAACA AAGATTCTGT GATTCCTACT CTTGAAAGTG TGGTCTTTGG TATTAACTAC
    ACAAAGCAGT TATCACCAGA CGGCTGCAGC TTTGTCATCG CAGATTCCTT CCTGCGTCAC
    GCCTACCGTT TCCACTACAC CCTCTGCGCT GCCCTGCTGC TGGCCTTCAA GGGGCTGCAC
    AGCTACTTCA TTATGGTAAC AGAAGAGCTC CCCTCTTGTC AGAAACTAGA ACTGGCCAAA
    GCCAACATGC AGGTCCTTTA TGAGCGCCTT CTCAGAAGAA AACAGCCAGC AACCCAGAAA
    GACACCTGTC TAGAGGAAAT GGATGTAGAA GCTCGACTTA CTGAATTATG TGAAGAAGTT
    AAGAAAATAG AAAATCCTGA TGAACTGGCA GAACTTATAA ATATGAATCT TGCACAACTT
    TGCTCACTTT TAATGGCTTT ATGGGGACAA TTTCTGGAAG TTATAACACT ACATGAGGAA
    CTAAGACTAT TACTAGCACA AGAGCACCAT ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC
    TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT TCATGCTCAG
    AGTCATCTCC AGATGTGCAC CGCTATCAAA AACACTTCCT TCTGCAGCTC TCTCCCACCC
    CTGCCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CGTTACCTAT TATCTTTGAA
    GATAGGTATT TAGATTCAGT TACTGAAGAC TTAGATGCAC CCTGGATGGG AATTCAGAGT
    CTTCAGAGAT CAGAGTCCAG TAGAATGGAT AAATGTGAGA CTGAAGAAAG CACTCTAGCT
    GGACTTTCCA GCCCAGAGTT GAAAATCAGA CCTGCTGGGA CCTCCAGTTT TTGGTCTACA
    GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA AAAGGAAAGA ATGAAGAATC AAATAAATCC
    AAGGTTAAGG TTACTAAGCT CATGAAAACA ATGAAACCTG AAAACACAAA AAAATTAATA
    AAACAGAACT CTAAGGATTT GGTGGTTTTG GTAGGCTACA AATGCTTGAA AAGTACAGCA
    TCAAATGATC TCTTTAAAAG CTTTGAAGGC AACCCTACAC ATAGTCAGAA GGAAGGTCTG
    GACCCCACAA TATATGGATA TAATTTTGAT CCAAAGACCT ACATGAGACA GACAAGTCAA
    AAGGAAGCTA GCTATTTGCC AGCTAATACA GAAAGAACTG AACAAAAGTC TCCAGATATT
    GAAAATGTGC AACCAGACCT GTTTGATCCT TTGAACTCTG GCAGCCTAAA TCTTTGTGCA
    AATTTGTCCA TTTCAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852642.1
    CDS142..4488
    Translation

    Target ORF information:

    RefSeq Version XM_024996874.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCAGCAGA TTGAAGAAAC CCTTGAGGAA ATGAATTTTC TGTTATCCCT GGATCTACAC 
    TTCACAGATG GAGATTATTC GGCAGATGAT CTGAATGCAT TGCAGCTAAT AAGCAGCCGC
    ACACTGAAGC TGCACTTCAG CCTCCAGAGA GGCCTCCATC ACCACGTTAA CGTCATGTTT
    GATTATTTCC ACCTTTCTGT TGTGTCTGTT ACAGTCCATG CGTCACTGGT TGCGCTACAC
    CAGCCACTGA TAAGTTTTCC TCGTCCTGTG AAGACAACTT GGTTAAACAG AAATGCACCA
    GCACAAAACA AAGATTCTGT GATTCCTACT CTTGAAAGTG TGGTCTTTGG TATTAACTAC
    ACAAAGCAGT TATCACCAGA CGGCTGCAGC TTTGTCATCG CAGATTCCTT CCTGCGTCAC
    GCCTACCGTT TCCACTACAC CCTCTGCGCT GCCCTGCTGC TGGCCTTCAA GGGGCTGCAC
    AGCTACTTCA TTATGGTAAC AGAAGAGCTC CCCTCTTGTC AGAAACTAGA ACTGGCCAAA
    GCCAACATGC AGGTCCTTTA TGAGCGCCTT CTCAGAAGAA AACAGCCAGC AACCCAGAAA
    GACACCTGTC TAGAGGAAAT GGATGTAGAA GCTCGACTTA CTGAATTATG TGAAGAAGTT
    AAGAAAATAG AAAATCCTGA TGAACTGGCA GAACTTATAA ATATGAATCT TGCACAACTT
    TGCTCACTTT TAATGGCTTT ATGGGGACAA TTTCTGGAAG TTATAACACT ACATGAGGAA
    CTAAGACTAT TACTAGCACA AGAGCACCAT ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC
    TTCTTTTGTT TTGAACATCC AAGAGAAGCT GCCATTGCAT ACCAGGAACT TCATGCTCAG
    AGTCATCTCC AGATGTGCAC CGCTATCAAA AACACTTCCT TCTGCAGCTC TCTCCCACCC
    CTGCCTATTG AATGTAGTGA ATTAGATGGA GATCTCAATT CGTTACCTAT TATCTTTGAA
    GATAGGTATT TAGATTCAGT TACTGAAGAC TTAGATGCAC CCTGGATGGG AATTCAGAGT
    CTTCAGAGAT CAGAGTCCAG TAGAATGGAT AAATGTGAGA CTGAAGAAAG CACTCTAGCT
    GGACTTTCCA GCCCAGAGTT GAAAATCAGA CCTGCTGGGA CCTCCAGTTT TTGGTCTACA
    GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA AAAGGAAAGA ATGAAGAATC AAATAAATCC
    AAGGTTAAGG TTACTAAGCT CATGAAAACA ATGAAACCTG AAAACACAAA AAAATTAATA
    AAACAGAACT CTAAGGATTT GGTGGTTTTG GTAGGCTACA AATGCTTGAA AAGTACAGCA
    TCAAATGATC TCTTTAAAAG CTTTGAAGGC AACCCTACAC ATAGTCAGAA GGAAGGTCTG
    GACCCCACAA TATATGGATA TAATTTTGAT CCAAAGACCT ACATGAGACA GACAAGTCAA
    AAGGAAGCTA GCTATTTGCC AGCTAATACA GAAAGAACTG AACAAAAGTC TCCAGATATT
    GAAAATGTGC AACCAGACCT GTTTGATCCT TTGAACTCTG GCAGCCTAAA TCTTTGTGCA
    AATTTGTCCA TTTCAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237929
    Clone ID Related Accession (Same CDS sequence) XM_024996875.1
    Accession Version XM_024996875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACTTTAG CCTTTCCAGC TTCAGTTCAT GATTCTCTGA TTTGCAGTAA AACATTTCAA 
    ATTCTATATA AAAATGAGGA GGTTGTTTTA AATGATGTCA TGATCTTCAA AGTTAAAATG
    TTGTTGGATG AAAAAAAGAT TGAAGAAACC CTTGAGGAAA TGAATTTTCT GTTATCCCTG
    GATCTACACT TCACAGATGG AGATTATTCG GCAGATGATC TGAATGCATT GCAGCTAATA
    AGCAGCCGCA CACTGAAGCT GCACTTCAGC CTCCAGAGAG GCCTCCATCA CCACGTTAAC
    GTCATGTTTG ATTATTTCCA CCTTTCTGTT GTGTCTGTTA CAGTCCATGC GTCACTGGTT
    GCGCTACACC AGCCACTGAT AAGTTTTCCT CGTCCTGTGA AGACAACTTG GTTAAACAGA
    AATGCACCAG CACAAAACAA AGATTCTGTG ATTCCTACTC TTGAAAGTGT GGTCTTTGGT
    ATTAACTACA CAAAGCAGTT ATCACCAGAC GGCTGCAGCT TTGTCATCGC AGATTCCTTC
    CTGCGTCACG CCTACCGTTT CCACTACACC CTCTGCGCTG CCCTGCTGCT GGCCTTCAAG
    GGGCTGCACA GCTACTTCAT TATGGTAACA GAAGAGCTCC CCTCTTGTCA GAAACTAGAA
    CTGGCCAAAG CCAACATGCA GGTCCTTTAT GAGCGCCTTC TCAGAAGAAA ACAGCCAGCA
    ACCCAGAAAG ACACCTGTCT AGAGGAAATG GATGTAGAAG CTCGACTTAC TGAATTATGT
    GAAGAAGTTA AGAAAATAGA AAATCCTGAT GAACTGGCAG AACTTATAAA TATGAATCTT
    GCACAACTTT GCTCACTTTT AATGGCTTTA TGGGGACAAT TTCTGGAAGT TATAACACTA
    CATGAGGAAC TAAGACTATT ACTAGCACAA GAGCACCATA CTCTGAGGGT GCGCAGGTTT
    TCTGAGGCCT TCTTTTGTTT TGAACATCCA AGAGAAGCTG CCATTGCATA CCAGGAACTT
    CATGCTCAGA GTCATCTCCA GATGTGCACC GCTATCAAAA ACACTTCCTT CTGCAGCTCT
    CTCCCACCCC TGCCTATTGA ATGTAGTGAA TTAGATGGAG ATCTCAATTC GTTACCTATT
    ATCTTTGAAG ATAGGTATTT AGATTCAGTT ACTGAAGGTA AACTTGATAT CTCCCAGGAT
    GATAGTGAAA TTGCACAAGT GGAACACAGT GTGGCATCCA GAAGCTCATC AGACGATTGC
    CATGATCGTC AGTCTGCCCC CACTTCAGGA GTTAGGACAA TTGAAGTTAA GCCATGTAAT
    AAAGACTGTT TCAGTGGAGA GAAAGTAACT GTTAAAATAG GACCTTGGAC AGAGCTTCAA
    CAAGATGAAA TATTTGTGGA TAATTTACAA CTGCCCAATT TTGAGTCCTT AGACTCTAAT
    GGTAAATCTA AACCTATGGA AATAACACTT GAAAAGGAAG CTTTGCAGGA AGCTAAGTGT
    CATTCTGTCG GGGAATCGTT AGCTAAGTTA AGAAATAATC AACCTGCTTC TACAAGAGAA
    TACCACCTTG TAGTGAGTGG AGACACCATT AAGTTACCAG ACATTAGTGC CACGTGTGCC
    TCATCTCGGT TCTCAGACTC AGGTGTGGAA AGTGAACCAA GCTCTGTTGC AGCACATCCA
    AACCCTGACA TAGTCTTTGA AACTGTACCA GGGCAAGGTC TTTTCATCAG TGAAAGGTTA
    TTTCCTCAGC TCTTGATGAA GCCTGACTGT AATGTAAAGT TTTCACTAGG CAGTCACTGT
    ACTGAGAGTA CAAGTGCTTT CAGTGAGATC CAGTCGTCCT TAACCTCCAT AAATTCTCTG
    CCTTCTGATG ACGAGCTGTC ACCTGATGAA AATCCTAAGA AATCTGCTGT ATCTGAATGC
    CACCTAAGTG ATAGCAAAAC TGTGTTAAAT CTAGGAACAA CTGATGTGCC AAAATGTGAC
    GATAGGAAAA AGTCAAGTAT TATTTTGCAG CAGCAGAGTG TCATATTTTC AGGGCATTTG
    GACAATGAAA CTATAGAAAT ACATTCCTTA AATTCAAGCA CTAAAGACCC TTTACAATTT
    GTTTTTTCAG ATGAAGATAC TTCCAGTGAT GTGAAAAGTA GTTGTAGCTC CAAACCTAAC
    TTGGACACTG TGTGTAAAGA CTCCCAGAGT CCTGATAAAT CCAGTGACTC TGTAGGAACC
    ACAGTTCCAT TAAGTTCAGA ACTGGCTTGT TCAGGCACCC CTTGTGTCGT TTCAGGTTCC
    GTTTCCACCA GGGCAGATAG CAGTGAAGAT AGAACTGTGA AAAGGAAAAA CAGCGATGCA
    TTAAATCCCA AACAAATGTG TTCAGAAGCC CCTGCAGTTA AAGATGAAAC TTACCTGGGT
    ACAGGTGATG CTTTTTCAGC CAGTCCTGAT ATGGTAAAGC AAGGGCTTGT GGAAAATTAT
    TTTGGCTGTC AAAGCAGTAC GGATGTTTCT GACACATGTG CTATTAGCTA CAGCCATTCA
    GTTAGCCCTC AGAAAGAAAC TTCTGGAAAA GAAATGAGTA GTCCTCAGCG GGATCAGGGT
    AAAGATGAGG AAGAGGAAGA GCAGGATCAA CAAATGGTTC AAAACGGGTA TTATGAGGAG
    GCAGATTACT CCTCTCTGGA GGCCGCGGGA GATGCTCACT GTGCAGATGG AGCTGACCTG
    GGAGGAGAAA GACTTGCCAA GTCTGAAAGA CTGGCCGGCG ACTATCTGAG CGATGGCATA
    ACCATGCCTA CTGTCTGTAC TTCTGGCTGT CTGTCCTTCC CGTCTGCACC ACGAGAGTCC
    CCTTGTGGCG TTAAGTACTC TTCTAAGAGT AAGTGTGACG CTGTTACCAA GCAGCCGAGC
    AGCGCCTCCC ACAGCTGCAC GTCATCTCTG TCCTGGTACG AAAACTCGCC AAAGCCTCAG
    ATACAAGCCT TCCTTCAGGC AAAAGATGAA TTGAAGCAAC TTAAACTCCC TGGGTTCATG
    TACAGCGATG TTCCTCTGCT GGCATCCTCA GTCCCTTATT TTTGCGTGGA GGAGGAGGAG
    GAGGACCATT GTGGAAATGG AGTGCATCTC ATTGTCTGTG TGCATGGCCT AGACGGTAAC
    AGTGCAGATC TCCGATTAGT AAAAACTTAC ATTGAACTTG GGCTTCCTGG GGAAAGAATT
    GATTTTCTTA TGTCTGAAAG AAATCAGAAT GATACTTTTG CTGATTTTGA TAGCATGACT
    GATCGCCTTT TGGATGAGAT AATACAATAT ATTCAGATAT ATAGTCTAAC AGTCTCAAAA
    ATAAGCTTTA TTGGACATTC TTTGGGCAAT TTAATAATCC GTTCAGTGCT TACAAGACCA
    CGGTTTAAAT ATTACCTCAG CAGGCTTCAT ACCTTTCTTT CACTTTCTGG ACCTCACCTT
    GGTACACTCT ACAATAGCAG TGCTCTTGTT AATACAGGTC TGTGGTTTAT GCAGAAATGG
    AAAAAATCAG GTTCTCTTTT ACAATTGACA TGTCGTGATC ATTCAGACCC TCGCCAAACT
    TTCTTATACA AGCTTAGTAA TAAAGCAGGG CTTCACTACT TCAGAAATGT TGTCCTAGTG
    GGATCTCTGC AAGATCGCTA TGTTCCTTAT CATTCTGCCC GCATTGAAAT GTGCAAGACA
    GCTTTGAAGG ACAAACAGTC AGGACAGATC TATTCAGAAA TGATCCACAA CTTACTTCGC
    CCGGTTCTGC AAAGCAAGGA CTGTAACTTG GTTCGATATA ATGTCATCAA TGCGTTGCCC
    AATACAGCTG ATTCACTCAT TGGCAGAGCT GCACATATAG CTGTTCTGGA TTCAGAAATA
    TTTTTAGAGA AATTCTTTCT GGTTGCTGCC CTCAAATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852643.1
    CDS164..4051
    Translation

    Target ORF information:

    RefSeq Version XM_024996875.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACTTTAG CCTTTCCAGC TTCAGTTCAT GATTCTCTGA TTTGCAGTAA AACATTTCAA 
    ATTCTATATA AAAATGAGGA GGTTGTTTTA AATGATGTCA TGATCTTCAA AGTTAAAATG
    TTGTTGGATG AAAAAAAGAT TGAAGAAACC CTTGAGGAAA TGAATTTTCT GTTATCCCTG
    GATCTACACT TCACAGATGG AGATTATTCG GCAGATGATC TGAATGCATT GCAGCTAATA
    AGCAGCCGCA CACTGAAGCT GCACTTCAGC CTCCAGAGAG GCCTCCATCA CCACGTTAAC
    GTCATGTTTG ATTATTTCCA CCTTTCTGTT GTGTCTGTTA CAGTCCATGC GTCACTGGTT
    GCGCTACACC AGCCACTGAT AAGTTTTCCT CGTCCTGTGA AGACAACTTG GTTAAACAGA
    AATGCACCAG CACAAAACAA AGATTCTGTG ATTCCTACTC TTGAAAGTGT GGTCTTTGGT
    ATTAACTACA CAAAGCAGTT ATCACCAGAC GGCTGCAGCT TTGTCATCGC AGATTCCTTC
    CTGCGTCACG CCTACCGTTT CCACTACACC CTCTGCGCTG CCCTGCTGCT GGCCTTCAAG
    GGGCTGCACA GCTACTTCAT TATGGTAACA GAAGAGCTCC CCTCTTGTCA GAAACTAGAA
    CTGGCCAAAG CCAACATGCA GGTCCTTTAT GAGCGCCTTC TCAGAAGAAA ACAGCCAGCA
    ACCCAGAAAG ACACCTGTCT AGAGGAAATG GATGTAGAAG CTCGACTTAC TGAATTATGT
    GAAGAAGTTA AGAAAATAGA AAATCCTGAT GAACTGGCAG AACTTATAAA TATGAATCTT
    GCACAACTTT GCTCACTTTT AATGGCTTTA TGGGGACAAT TTCTGGAAGT TATAACACTA
    CATGAGGAAC TAAGACTATT ACTAGCACAA GAGCACCATA CTCTGAGGGT GCGCAGGTTT
    TCTGAGGCCT TCTTTTGTTT TGAACATCCA AGAGAAGCTG CCATTGCATA CCAGGAACTT
    CATGCTCAGA GTCATCTCCA GATGTGCACC GCTATCAAAA ACACTTCCTT CTGCAGCTCT
    CTCCCACCCC TGCCTATTGA ATGTAGTGAA TTAGATGGAG ATCTCAATTC GTTACCTATT
    ATCTTTGAAG ATAGGTATTT AGATTCAGTT ACTGAAGGTA AACTTGATAT CTCCCAGGAT
    GATAGTGAAA TTGCACAAGT GGAACACAGT GTGGCATCCA GAAGCTCATC AGACGATTGC
    CATGATCGTC AGTCTGCCCC CACTTCAGGA GTTAGGACAA TTGAAGTTAA GCCATGTAAT
    AAAGACTGTT TCAGTGGAGA GAAAGTAACT GTTAAAATAG GACCTTGGAC AGAGCTTCAA
    CAAGATGAAA TATTTGTGGA TAATTTACAA CTGCCCAATT TTGAGTCCTT AGACTCTAAT
    GGTAAATCTA AACCTATGGA AATAACACTT GAAAAGGAAG CTTTGCAGGA AGCTAAGTGT
    CATTCTGTCG GGGAATCGTT AGCTAAGTTA AGAAATAATC AACCTGCTTC TACAAGAGAA
    TACCACCTTG TAGTGAGTGG AGACACCATT AAGTTACCAG ACATTAGTGC CACGTGTGCC
    TCATCTCGGT TCTCAGACTC AGGTGTGGAA AGTGAACCAA GCTCTGTTGC AGCACATCCA
    AACCCTGACA TAGTCTTTGA AACTGTACCA GGGCAAGGTC TTTTCATCAG TGAAAGGTTA
    TTTCCTCAGC TCTTGATGAA GCCTGACTGT AATGTAAAGT TTTCACTAGG CAGTCACTGT
    ACTGAGAGTA CAAGTGCTTT CAGTGAGATC CAGTCGTCCT TAACCTCCAT AAATTCTCTG
    CCTTCTGATG ACGAGCTGTC ACCTGATGAA AATCCTAAGA AATCTGCTGT ATCTGAATGC
    CACCTAAGTG ATAGCAAAAC TGTGTTAAAT CTAGGAACAA CTGATGTGCC AAAATGTGAC
    GATAGGAAAA AGTCAAGTAT TATTTTGCAG CAGCAGAGTG TCATATTTTC AGGGCATTTG
    GACAATGAAA CTATAGAAAT ACATTCCTTA AATTCAAGCA CTAAAGACCC TTTACAATTT
    GTTTTTTCAG ATGAAGATAC TTCCAGTGAT GTGAAAAGTA GTTGTAGCTC CAAACCTAAC
    TTGGACACTG TGTGTAAAGA CTCCCAGAGT CCTGATAAAT CCAGTGACTC TGTAGGAACC
    ACAGTTCCAT TAAGTTCAGA ACTGGCTTGT TCAGGCACCC CTTGTGTCGT TTCAGGTTCC
    GTTTCCACCA GGGCAGATAG CAGTGAAGAT AGAACTGTGA AAAGGAAAAA CAGCGATGCA
    TTAAATCCCA AACAAATGTG TTCAGAAGCC CCTGCAGTTA AAGATGAAAC TTACCTGGGT
    ACAGGTGATG CTTTTTCAGC CAGTCCTGAT ATGGTAAAGC AAGGGCTTGT GGAAAATTAT
    TTTGGCTGTC AAAGCAGTAC GGATGTTTCT GACACATGTG CTATTAGCTA CAGCCATTCA
    GTTAGCCCTC AGAAAGAAAC TTCTGGAAAA GAAATGAGTA GTCCTCAGCG GGATCAGGGT
    AAAGATGAGG AAGAGGAAGA GCAGGATCAA CAAATGGTTC AAAACGGGTA TTATGAGGAG
    GCAGATTACT CCTCTCTGGA GGCCGCGGGA GATGCTCACT GTGCAGATGG AGCTGACCTG
    GGAGGAGAAA GACTTGCCAA GTCTGAAAGA CTGGCCGGCG ACTATCTGAG CGATGGCATA
    ACCATGCCTA CTGTCTGTAC TTCTGGCTGT CTGTCCTTCC CGTCTGCACC ACGAGAGTCC
    CCTTGTGGCG TTAAGTACTC TTCTAAGAGT AAGTGTGACG CTGTTACCAA GCAGCCGAGC
    AGCGCCTCCC ACAGCTGCAC GTCATCTCTG TCCTGGTACG AAAACTCGCC AAAGCCTCAG
    ATACAAGCCT TCCTTCAGGC AAAAGATGAA TTGAAGCAAC TTAAACTCCC TGGGTTCATG
    TACAGCGATG TTCCTCTGCT GGCATCCTCA GTCCCTTATT TTTGCGTGGA GGAGGAGGAG
    GAGGACCATT GTGGAAATGG AGTGCATCTC ATTGTCTGTG TGCATGGCCT AGACGGTAAC
    AGTGCAGATC TCCGATTAGT AAAAACTTAC ATTGAACTTG GGCTTCCTGG GGAAAGAATT
    GATTTTCTTA TGTCTGAAAG AAATCAGAAT GATACTTTTG CTGATTTTGA TAGCATGACT
    GATCGCCTTT TGGATGAGAT AATACAATAT ATTCAGATAT ATAGTCTAAC AGTCTCAAAA
    ATAAGCTTTA TTGGACATTC TTTGGGCAAT TTAATAATCC GTTCAGTGCT TACAAGACCA
    CGGTTTAAAT ATTACCTCAG CAGGCTTCAT ACCTTTCTTT CACTTTCTGG ACCTCACCTT
    GGTACACTCT ACAATAGCAG TGCTCTTGTT AATACAGGTC TGTGGTTTAT GCAGAAATGG
    AAAAAATCAG GTTCTCTTTT ACAATTGACA TGTCGTGATC ATTCAGACCC TCGCCAAACT
    TTCTTATACA AGCTTAGTAA TAAAGCAGGG CTTCACTACT TCAGAAATGT TGTCCTAGTG
    GGATCTCTGC AAGATCGCTA TGTTCCTTAT CATTCTGCCC GCATTGAAAT GTGCAAGACA
    GCTTTGAAGG ACAAACAGTC AGGACAGATC TATTCAGAAA TGATCCACAA CTTACTTCGC
    CCGGTTCTGC AAAGCAAGGA CTGTAACTTG GTTCGATATA ATGTCATCAA TGCGTTGCCC
    AATACAGCTG ATTCACTCAT TGGCAGAGCT GCACATATAG CTGTTCTGGA TTCAGAAATA
    TTTTTAGAGA AATTCTTTCT GGTTGCTGCC CTCAAATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22442
    Clone ID Related Accession (Same CDS sequence) XM_002690006.4 , XM_015464712.1 , XM_002690006.5
    Accession Version XM_002690006.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104165.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT
    AAACTTGATA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC
    AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA
    ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA
    GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAT
    TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA
    GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT
    CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA
    GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAACCA
    AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT
    CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTCTTGATGA AGCCTGACTG TAATGTAAAG
    TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC
    TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG
    AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA
    ACTGATGTGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT
    GTCATATTTT CAGGGCATTT GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC
    ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCCAGTGA TGTGAAAAGT
    AGTTGTAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA
    TCCAGTGACT CTGTAGGAAC CACAGTTCCA TTAAGTTCAG AACTGGCTTG TTCAGGCACC
    CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG
    AAAAGGAAAA ACAGCGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT
    AAAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG
    CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGT
    GCTATTAGCT ACAGCCATTC AGTTAGCCCT CAGAAAGAAA CTTCTGGAAA AGAAATGAGT
    AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT
    CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC
    TGTGCAGATG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC
    GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC
    CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAGTGTGAC
    GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CGTCATCTCT GTCCTGGTAC
    GAAAACTCGC CAAAGCCTCA GATACAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA
    CTTAAACTCC CTGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT
    TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TGTGGAAATG GAGTGCATCT CATTGTCTGT
    GTGCATGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT
    GGGCTTCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT
    GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA
    TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC
    CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT
    TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT
    CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT
    CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC
    TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC
    CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA
    ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT
    AATGTCATCA ATGCGTTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA
    GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT
    TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690052.1
    CDS335..4303
    Translation

    Target ORF information:

    RefSeq Version XM_002690006.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690006.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT
    AAACTTGATA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC
    AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA
    ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA
    GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAT
    TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA
    GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT
    CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA
    GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAACCA
    AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT
    CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTCTTGATGA AGCCTGACTG TAATGTAAAG
    TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC
    TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG
    AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA
    ACTGATGTGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT
    GTCATATTTT CAGGGCATTT GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC
    ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCCAGTGA TGTGAAAAGT
    AGTTGTAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA
    TCCAGTGACT CTGTAGGAAC CACAGTTCCA TTAAGTTCAG AACTGGCTTG TTCAGGCACC
    CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG
    AAAAGGAAAA ACAGCGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT
    AAAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG
    CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGT
    GCTATTAGCT ACAGCCATTC AGTTAGCCCT CAGAAAGAAA CTTCTGGAAA AGAAATGAGT
    AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT
    CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC
    TGTGCAGATG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC
    GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC
    CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAGTGTGAC
    GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CGTCATCTCT GTCCTGGTAC
    GAAAACTCGC CAAAGCCTCA GATACAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA
    CTTAAACTCC CTGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT
    TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TGTGGAAATG GAGTGCATCT CATTGTCTGT
    GTGCATGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT
    GGGCTTCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT
    GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA
    TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC
    CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT
    TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT
    CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT
    CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC
    TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC
    CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA
    ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT
    AATGTCATCA ATGCGTTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA
    GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT
    TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22442
    Clone ID Related Accession (Same CDS sequence) XM_002690006.4 , XM_015464712.1 , XM_002690006.5
    Accession Version XM_015464712.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645510.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT
    AAACTTGATA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC
    AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA
    ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA
    GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAT
    TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA
    GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT
    CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA
    GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAACCA
    AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT
    CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTCTTGATGA AGCCTGACTG TAATGTAAAG
    TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC
    TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG
    AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA
    ACTGATGTGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT
    GTCATATTTT CAGGGCATTT GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC
    ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCCAGTGA TGTGAAAAGT
    AGTTGTAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA
    TCCAGTGACT CTGTAGGAAC CACAGTTCCA TTAAGTTCAG AACTGGCTTG TTCAGGCACC
    CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG
    AAAAGGAAAA ACAGCGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT
    AAAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG
    CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGT
    GCTATTAGCT ACAGCCATTC AGTTAGCCCT CAGAAAGAAA CTTCTGGAAA AGAAATGAGT
    AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT
    CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC
    TGTGCAGATG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC
    GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC
    CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAGTGTGAC
    GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CGTCATCTCT GTCCTGGTAC
    GAAAACTCGC CAAAGCCTCA GATACAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA
    CTTAAACTCC CTGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT
    TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TGTGGAAATG GAGTGCATCT CATTGTCTGT
    GTGCATGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT
    GGGCTTCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT
    GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA
    TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC
    CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT
    TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT
    CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT
    CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC
    TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC
    CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA
    ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT
    AATGTCATCA ATGCGTTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA
    GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT
    TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320198.1
    CDS335..4303
    Translation

    Target ORF information:

    RefSeq Version XM_015464712.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464712.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT
    AAACTTGATA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC
    AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA
    ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA
    GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAT
    TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA
    GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT
    CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA
    GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAACCA
    AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT
    CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTCTTGATGA AGCCTGACTG TAATGTAAAG
    TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC
    TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG
    AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA
    ACTGATGTGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT
    GTCATATTTT CAGGGCATTT GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC
    ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCCAGTGA TGTGAAAAGT
    AGTTGTAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA
    TCCAGTGACT CTGTAGGAAC CACAGTTCCA TTAAGTTCAG AACTGGCTTG TTCAGGCACC
    CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG
    AAAAGGAAAA ACAGCGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT
    AAAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG
    CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGT
    GCTATTAGCT ACAGCCATTC AGTTAGCCCT CAGAAAGAAA CTTCTGGAAA AGAAATGAGT
    AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT
    CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC
    TGTGCAGATG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC
    GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC
    CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAGTGTGAC
    GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CGTCATCTCT GTCCTGGTAC
    GAAAACTCGC CAAAGCCTCA GATACAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA
    CTTAAACTCC CTGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT
    TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TGTGGAAATG GAGTGCATCT CATTGTCTGT
    GTGCATGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT
    GGGCTTCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT
    GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA
    TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC
    CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT
    TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT
    CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT
    CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC
    TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC
    CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA
    ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT
    AATGTCATCA ATGCGTTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA
    GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT
    TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22442
    Clone ID Related Accession (Same CDS sequence) XM_002690006.4 , XM_015464712.1 , XM_002690006.5
    Accession Version XM_002690006.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690006.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT
    AAACTTGATA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC
    AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA
    ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA
    GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAT
    TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA
    GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT
    CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA
    GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAACCA
    AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT
    CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTCTTGATGA AGCCTGACTG TAATGTAAAG
    TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC
    TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG
    AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA
    ACTGATGTGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT
    GTCATATTTT CAGGGCATTT GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC
    ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCCAGTGA TGTGAAAAGT
    AGTTGTAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA
    TCCAGTGACT CTGTAGGAAC CACAGTTCCA TTAAGTTCAG AACTGGCTTG TTCAGGCACC
    CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG
    AAAAGGAAAA ACAGCGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT
    AAAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG
    CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGT
    GCTATTAGCT ACAGCCATTC AGTTAGCCCT CAGAAAGAAA CTTCTGGAAA AGAAATGAGT
    AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT
    CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC
    TGTGCAGATG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC
    GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC
    CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAGTGTGAC
    GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CGTCATCTCT GTCCTGGTAC
    GAAAACTCGC CAAAGCCTCA GATACAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA
    CTTAAACTCC CTGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT
    TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TGTGGAAATG GAGTGCATCT CATTGTCTGT
    GTGCATGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT
    GGGCTTCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT
    GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA
    TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC
    CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT
    TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT
    CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT
    CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC
    TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC
    CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA
    ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT
    AATGTCATCA ATGCGTTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA
    GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT
    TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690052.1
    CDS372..4340
    Translation

    Target ORF information:

    RefSeq Version XM_002690006.5
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690006.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGGT
    AAACTTGATA TCTCCCAGGA TGATAGTGAA ATTGCACAAG TGGAACACAG TGTGGCATCC
    AGAAGCTCAT CAGACGATTG CCATGATCGT CAGTCTGCCC CCACTTCAGG AGTTAGGACA
    ATTGAAGTTA AGCCATGTAA TAAAGACTGT TTCAGTGGAG AGAAAGTAAC TGTTAAAATA
    GGACCTTGGA CAGAGCTTCA ACAAGATGAA ATATTTGTGG ATAATTTACA ACTGCCCAAT
    TTTGAGTCCT TAGACTCTAA TGGTAAATCT AAACCTATGG AAATAACACT TGAAAAGGAA
    GCTTTGCAGG AAGCTAAGTG TCATTCTGTC GGGGAATCGT TAGCTAAGTT AAGAAATAAT
    CAACCTGCTT CTACAAGAGA ATACCACCTT GTAGTGAGTG GAGACACCAT TAAGTTACCA
    GACATTAGTG CCACGTGTGC CTCATCTCGG TTCTCAGACT CAGGTGTGGA AAGTGAACCA
    AGCTCTGTTG CAGCACATCC AAACCCTGAC ATAGTCTTTG AAACTGTACC AGGGCAAGGT
    CTTTTCATCA GTGAAAGGTT ATTTCCTCAG CTCTTGATGA AGCCTGACTG TAATGTAAAG
    TTTTCACTAG GCAGTCACTG TACTGAGAGT ACAAGTGCTT TCAGTGAGAT CCAGTCGTCC
    TTAACCTCCA TAAATTCTCT GCCTTCTGAT GACGAGCTGT CACCTGATGA AAATCCTAAG
    AAATCTGCTG TATCTGAATG CCACCTAAGT GATAGCAAAA CTGTGTTAAA TCTAGGAACA
    ACTGATGTGC CAAAATGTGA CGATAGGAAA AAGTCAAGTA TTATTTTGCA GCAGCAGAGT
    GTCATATTTT CAGGGCATTT GGACAATGAA ACTATAGAAA TACATTCCTT AAATTCAAGC
    ACTAAAGACC CTTTACAATT TGTTTTTTCA GATGAAGATA CTTCCAGTGA TGTGAAAAGT
    AGTTGTAGCT CCAAACCTAA CTTGGACACT GTGTGTAAAG ACTCCCAGAG TCCTGATAAA
    TCCAGTGACT CTGTAGGAAC CACAGTTCCA TTAAGTTCAG AACTGGCTTG TTCAGGCACC
    CCTTGTGTCG TTTCAGGTTC CGTTTCCACC AGGGCAGATA GCAGTGAAGA TAGAACTGTG
    AAAAGGAAAA ACAGCGATGC ATTAAATCCC AAACAAATGT GTTCAGAAGC CCCTGCAGTT
    AAAGATGAAA CTTACCTGGG TACAGGTGAT GCTTTTTCAG CCAGTCCTGA TATGGTAAAG
    CAAGGGCTTG TGGAAAATTA TTTTGGCTGT CAAAGCAGTA CGGATGTTTC TGACACATGT
    GCTATTAGCT ACAGCCATTC AGTTAGCCCT CAGAAAGAAA CTTCTGGAAA AGAAATGAGT
    AGTCCTCAGC GGGATCAGGG TAAAGATGAG GAAGAGGAAG AGCAGGATCA ACAAATGGTT
    CAAAACGGGT ATTATGAGGA GGCAGATTAC TCCTCTCTGG AGGCCGCGGG AGATGCTCAC
    TGTGCAGATG GAGCTGACCT GGGAGGAGAA AGACTTGCCA AGTCTGAAAG ACTGGCCGGC
    GACTATCTGA GCGATGGCAT AACCATGCCT ACTGTCTGTA CTTCTGGCTG TCTGTCCTTC
    CCGTCTGCAC CACGAGAGTC CCCTTGTGGC GTTAAGTACT CTTCTAAGAG TAAGTGTGAC
    GCTGTTACCA AGCAGCCGAG CAGCGCCTCC CACAGCTGCA CGTCATCTCT GTCCTGGTAC
    GAAAACTCGC CAAAGCCTCA GATACAAGCC TTCCTTCAGG CAAAAGATGA ATTGAAGCAA
    CTTAAACTCC CTGGGTTCAT GTACAGCGAT GTTCCTCTGC TGGCATCCTC AGTCCCTTAT
    TTTTGCGTGG AGGAGGAGGA GGAGGACCAT TGTGGAAATG GAGTGCATCT CATTGTCTGT
    GTGCATGGCC TAGACGGTAA CAGTGCAGAT CTCCGATTAG TAAAAACTTA CATTGAACTT
    GGGCTTCCTG GGGAAAGAAT TGATTTTCTT ATGTCTGAAA GAAATCAGAA TGATACTTTT
    GCTGATTTTG ATAGCATGAC TGATCGCCTT TTGGATGAGA TAATACAATA TATTCAGATA
    TATAGTCTAA CAGTCTCAAA AATAAGCTTT ATTGGACATT CTTTGGGCAA TTTAATAATC
    CGTTCAGTGC TTACAAGACC ACGGTTTAAA TATTACCTCA GCAGGCTTCA TACCTTTCTT
    TCACTTTCTG GACCTCACCT TGGTACACTC TACAATAGCA GTGCTCTTGT TAATACAGGT
    CTGTGGTTTA TGCAGAAATG GAAAAAATCA GGTTCTCTTT TACAATTGAC ATGTCGTGAT
    CATTCAGACC CTCGCCAAAC TTTCTTATAC AAGCTTAGTA ATAAAGCAGG GCTTCACTAC
    TTCAGAAATG TTGTCCTAGT GGGATCTCTG CAAGATCGCT ATGTTCCTTA TCATTCTGCC
    CGCATTGAAA TGTGCAAGAC AGCTTTGAAG GACAAACAGT CAGGACAGAT CTATTCAGAA
    ATGATCCACA ACTTACTTCG CCCGGTTCTG CAAAGCAAGG ACTGTAACTT GGTTCGATAT
    AATGTCATCA ATGCGTTGCC CAATACAGCT GATTCACTCA TTGGCAGAGC TGCACATATA
    GCTGTTCTGG ATTCAGAAAT ATTTTTAGAG AAATTCTTTC TGGTTGCTGC CCTCAAATAT
    TTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06014
    Clone ID Related Accession (Same CDS sequence) XM_002705105.4 , XM_005210636.4 , XM_015464709.1 , XM_005210636.3 , XM_010808337.2 , XM_024996871.1
    Accession Version XM_002705105.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645510.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002705105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002705151.2
    CDS476..5110
    Translation

    Target ORF information:

    RefSeq Version XM_002705105.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002705105.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06014
    Clone ID Related Accession (Same CDS sequence) XM_002705105.4 , XM_005210636.4 , XM_015464709.1 , XM_005210636.3 , XM_010808337.2 , XM_024996871.1
    Accession Version XM_005210636.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210636.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210693.1
    CDS404..5038
    Translation

    Target ORF information:

    RefSeq Version XM_005210636.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210636.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06014
    Clone ID Related Accession (Same CDS sequence) XM_002705105.4 , XM_005210636.4 , XM_015464709.1 , XM_005210636.3 , XM_010808337.2 , XM_024996871.1
    Accession Version XM_015464709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645510.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320195.1
    CDS340..4974
    Translation

    Target ORF information:

    RefSeq Version XM_015464709.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06014
    Clone ID Related Accession (Same CDS sequence) XM_002705105.4 , XM_005210636.4 , XM_015464709.1 , XM_005210636.3 , XM_010808337.2 , XM_024996871.1
    Accession Version XM_005210636.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104165.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210693.1
    CDS340..4974
    Translation

    Target ORF information:

    RefSeq Version XM_005210636.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210636.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06014
    Clone ID Related Accession (Same CDS sequence) XM_002705105.4 , XM_005210636.4 , XM_015464709.1 , XM_005210636.3 , XM_010808337.2 , XM_024996871.1
    Accession Version XM_010808337.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104165.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806639.1
    CDS476..5110
    Translation

    Target ORF information:

    RefSeq Version XM_010808337.2
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808337.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06014
    Clone ID Related Accession (Same CDS sequence) XM_002705105.4 , XM_005210636.4 , XM_015464709.1 , XM_005210636.3 , XM_010808337.2 , XM_024996871.1
    Accession Version XM_024996871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852639.1
    CDS460..5094
    Translation

    Target ORF information:

    RefSeq Version XM_024996871.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGACTT AGATGCACCC TGGATGGGAA TTCAGAGTCT TCAGAGATCA
    GAGTCCAGTA GAATGGATAA ATGTGAGACT GAAGAAAGCA CTCTAGCTGG ACTTTCCAGC
    CCAGAGTTGA AAATCAGACC TGCTGGGACC TCCAGTTTTT GGTCTACAGA AGGTGAAAAG
    CAGCTAACAA AGTCTCTAAA AGGAAAGAAT GAAGAATCAA ATAAATCCAA GGTTAAGGTT
    ACTAAGCTCA TGAAAACAAT GAAACCTGAA AACACAAAAA AATTAATAAA ACAGAACTCT
    AAGGATTTGG TGGTTTTGGT AGGCTACAAA TGCTTGAAAA GTACAGCATC AAATGATCTC
    TTTAAAAGCT TTGAAGGCAA CCCTACACAT AGTCAGAAGG AAGGTCTGGA CCCCACAATA
    TATGGATATA ATTTTGATCC AAAGACCTAC ATGAGACAGA CAAGTCAAAA GGAAGCTAGC
    TATTTGCCAG CTAATACAGA AAGAACTGAA CAAAAGTCTC CAGATATTGA AAATGTGCAA
    CCAGACCTGT TTGATCCTTT GAACTCTGGC AGCCTAAATC TTTGTGCAAA TTTGTCCATT
    TCAGGTAAAC TTGATATCTC CCAGGATGAT AGTGAAATTG CACAAGTGGA ACACAGTGTG
    GCATCCAGAA GCTCATCAGA CGATTGCCAT GATCGTCAGT CTGCCCCCAC TTCAGGAGTT
    AGGACAATTG AAGTTAAGCC ATGTAATAAA GACTGTTTCA GTGGAGAGAA AGTAACTGTT
    AAAATAGGAC CTTGGACAGA GCTTCAACAA GATGAAATAT TTGTGGATAA TTTACAACTG
    CCCAATTTTG AGTCCTTAGA CTCTAATGGT AAATCTAAAC CTATGGAAAT AACACTTGAA
    AAGGAAGCTT TGCAGGAAGC TAAGTGTCAT TCTGTCGGGG AATCGTTAGC TAAGTTAAGA
    AATAATCAAC CTGCTTCTAC AAGAGAATAC CACCTTGTAG TGAGTGGAGA CACCATTAAG
    TTACCAGACA TTAGTGCCAC GTGTGCCTCA TCTCGGTTCT CAGACTCAGG TGTGGAAAGT
    GAACCAAGCT CTGTTGCAGC ACATCCAAAC CCTGACATAG TCTTTGAAAC TGTACCAGGG
    CAAGGTCTTT TCATCAGTGA AAGGTTATTT CCTCAGCTCT TGATGAAGCC TGACTGTAAT
    GTAAAGTTTT CACTAGGCAG TCACTGTACT GAGAGTACAA GTGCTTTCAG TGAGATCCAG
    TCGTCCTTAA CCTCCATAAA TTCTCTGCCT TCTGATGACG AGCTGTCACC TGATGAAAAT
    CCTAAGAAAT CTGCTGTATC TGAATGCCAC CTAAGTGATA GCAAAACTGT GTTAAATCTA
    GGAACAACTG ATGTGCCAAA ATGTGACGAT AGGAAAAAGT CAAGTATTAT TTTGCAGCAG
    CAGAGTGTCA TATTTTCAGG GCATTTGGAC AATGAAACTA TAGAAATACA TTCCTTAAAT
    TCAAGCACTA AAGACCCTTT ACAATTTGTT TTTTCAGATG AAGATACTTC CAGTGATGTG
    AAAAGTAGTT GTAGCTCCAA ACCTAACTTG GACACTGTGT GTAAAGACTC CCAGAGTCCT
    GATAAATCCA GTGACTCTGT AGGAACCACA GTTCCATTAA GTTCAGAACT GGCTTGTTCA
    GGCACCCCTT GTGTCGTTTC AGGTTCCGTT TCCACCAGGG CAGATAGCAG TGAAGATAGA
    ACTGTGAAAA GGAAAAACAG CGATGCATTA AATCCCAAAC AAATGTGTTC AGAAGCCCCT
    GCAGTTAAAG ATGAAACTTA CCTGGGTACA GGTGATGCTT TTTCAGCCAG TCCTGATATG
    GTAAAGCAAG GGCTTGTGGA AAATTATTTT GGCTGTCAAA GCAGTACGGA TGTTTCTGAC
    ACATGTGCTA TTAGCTACAG CCATTCAGTT AGCCCTCAGA AAGAAACTTC TGGAAAAGAA
    ATGAGTAGTC CTCAGCGGGA TCAGGGTAAA GATGAGGAAG AGGAAGAGCA GGATCAACAA
    ATGGTTCAAA ACGGGTATTA TGAGGAGGCA GATTACTCCT CTCTGGAGGC CGCGGGAGAT
    GCTCACTGTG CAGATGGAGC TGACCTGGGA GGAGAAAGAC TTGCCAAGTC TGAAAGACTG
    GCCGGCGACT ATCTGAGCGA TGGCATAACC ATGCCTACTG TCTGTACTTC TGGCTGTCTG
    TCCTTCCCGT CTGCACCACG AGAGTCCCCT TGTGGCGTTA AGTACTCTTC TAAGAGTAAG
    TGTGACGCTG TTACCAAGCA GCCGAGCAGC GCCTCCCACA GCTGCACGTC ATCTCTGTCC
    TGGTACGAAA ACTCGCCAAA GCCTCAGATA CAAGCCTTCC TTCAGGCAAA AGATGAATTG
    AAGCAACTTA AACTCCCTGG GTTCATGTAC AGCGATGTTC CTCTGCTGGC ATCCTCAGTC
    CCTTATTTTT GCGTGGAGGA GGAGGAGGAG GACCATTGTG GAAATGGAGT GCATCTCATT
    GTCTGTGTGC ATGGCCTAGA CGGTAACAGT GCAGATCTCC GATTAGTAAA AACTTACATT
    GAACTTGGGC TTCCTGGGGA AAGAATTGAT TTTCTTATGT CTGAAAGAAA TCAGAATGAT
    ACTTTTGCTG ATTTTGATAG CATGACTGAT CGCCTTTTGG ATGAGATAAT ACAATATATT
    CAGATATATA GTCTAACAGT CTCAAAAATA AGCTTTATTG GACATTCTTT GGGCAATTTA
    ATAATCCGTT CAGTGCTTAC AAGACCACGG TTTAAATATT ACCTCAGCAG GCTTCATACC
    TTTCTTTCAC TTTCTGGACC TCACCTTGGT ACACTCTACA ATAGCAGTGC TCTTGTTAAT
    ACAGGTCTGT GGTTTATGCA GAAATGGAAA AAATCAGGTT CTCTTTTACA ATTGACATGT
    CGTGATCATT CAGACCCTCG CCAAACTTTC TTATACAAGC TTAGTAATAA AGCAGGGCTT
    CACTACTTCA GAAATGTTGT CCTAGTGGGA TCTCTGCAAG ATCGCTATGT TCCTTATCAT
    TCTGCCCGCA TTGAAATGTG CAAGACAGCT TTGAAGGACA AACAGTCAGG ACAGATCTAT
    TCAGAAATGA TCCACAACTT ACTTCGCCCG GTTCTGCAAA GCAAGGACTG TAACTTGGTT
    CGATATAATG TCATCAATGC GTTGCCCAAT ACAGCTGATT CACTCATTGG CAGAGCTGCA
    CATATAGCTG TTCTGGATTC AGAAATATTT TTAGAGAAAT TCTTTCTGGT TGCTGCCCTC
    AAATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06015
    Clone ID Related Accession (Same CDS sequence) XM_005210639.3 , XM_015464711.1 , XM_005210639.4
    Accession Version XM_005210639.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104165.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210696.1
    CDS335..4381
    Translation

    Target ORF information:

    RefSeq Version XM_005210639.3
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210639.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06015
    Clone ID Related Accession (Same CDS sequence) XM_005210639.3 , XM_015464711.1 , XM_005210639.4
    Accession Version XM_015464711.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645510.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320197.1
    CDS335..4381
    Translation

    Target ORF information:

    RefSeq Version XM_015464711.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06015
    Clone ID Related Accession (Same CDS sequence) XM_005210639.3 , XM_015464711.1 , XM_005210639.4
    Accession Version XM_005210639.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210639.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210696.1
    CDS371..4417
    Translation

    Target ORF information:

    RefSeq Version XM_005210639.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210639.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGCCAAAGC CAACATGCAG
    GTCCTTTATG AGCGCCTTCT CAGAAGAAAA CAGCCAGCAA CCCAGAAAGA CACCTGTCTA
    GAGGAAATGG ATGTAGAAGC TCGACTTACT GAATTATGTG AAGAAGTTAA GAAAATAGAA
    AATCCTGATG AACTGGCAGA ACTTATAAAT ATGAATCTTG CACAACTTTG CTCACTTTTA
    ATGGCTTTAT GGGGACAATT TCTGGAAGTT ATAACACTAC ATGAGGAACT AAGACTATTA
    CTAGCACAAG AGCACCATAC TCTGAGGGTG CGCAGGTTTT CTGAGGCCTT CTTTTGTTTT
    GAACATCCAA GAGAAGCTGC CATTGCATAC CAGGAACTTC ATGCTCAGAG TCATCTCCAG
    ATGTGCACCG CTATCAAAAA CACTTCCTTC TGCAGCTCTC TCCCACCCCT GCCTATTGAA
    TGTAGTGAAT TAGATGGAGA TCTCAATTCG TTACCTATTA TCTTTGAAGA TAGGTATTTA
    GATTCAGTTA CTGAAGGTAA ACTTGATATC TCCCAGGATG ATAGTGAAAT TGCACAAGTG
    GAACACAGTG TGGCATCCAG AAGCTCATCA GACGATTGCC ATGATCGTCA GTCTGCCCCC
    ACTTCAGGAG TTAGGACAAT TGAAGTTAAG CCATGTAATA AAGACTGTTT CAGTGGAGAG
    AAAGTAACTG TTAAAATAGG ACCTTGGACA GAGCTTCAAC AAGATGAAAT ATTTGTGGAT
    AATTTACAAC TGCCCAATTT TGAGTCCTTA GACTCTAATG GTAAATCTAA ACCTATGGAA
    ATAACACTTG AAAAGGAAGC TTTGCAGGAA GCTAAGTGTC ATTCTGTCGG GGAATCGTTA
    GCTAAGTTAA GAAATAATCA ACCTGCTTCT ACAAGAGAAT ACCACCTTGT AGTGAGTGGA
    GACACCATTA AGTTACCAGA CATTAGTGCC ACGTGTGCCT CATCTCGGTT CTCAGACTCA
    GGTGTGGAAA GTGAACCAAG CTCTGTTGCA GCACATCCAA ACCCTGACAT AGTCTTTGAA
    ACTGTACCAG GGCAAGGTCT TTTCATCAGT GAAAGGTTAT TTCCTCAGCT CTTGATGAAG
    CCTGACTGTA ATGTAAAGTT TTCACTAGGC AGTCACTGTA CTGAGAGTAC AAGTGCTTTC
    AGTGAGATCC AGTCGTCCTT AACCTCCATA AATTCTCTGC CTTCTGATGA CGAGCTGTCA
    CCTGATGAAA ATCCTAAGAA ATCTGCTGTA TCTGAATGCC ACCTAAGTGA TAGCAAAACT
    GTGTTAAATC TAGGAACAAC TGATGTGCCA AAATGTGACG ATAGGAAAAA GTCAAGTATT
    ATTTTGCAGC AGCAGAGTGT CATATTTTCA GGGCATTTGG ACAATGAAAC TATAGAAATA
    CATTCCTTAA ATTCAAGCAC TAAAGACCCT TTACAATTTG TTTTTTCAGA TGAAGATACT
    TCCAGTGATG TGAAAAGTAG TTGTAGCTCC AAACCTAACT TGGACACTGT GTGTAAAGAC
    TCCCAGAGTC CTGATAAATC CAGTGACTCT GTAGGAACCA CAGTTCCATT AAGTTCAGAA
    CTGGCTTGTT CAGGCACCCC TTGTGTCGTT TCAGGTTCCG TTTCCACCAG GGCAGATAGC
    AGTGAAGATA GAACTGTGAA AAGGAAAAAC AGCGATGCAT TAAATCCCAA ACAAATGTGT
    TCAGAAGCCC CTGCAGTTAA AGATGAAACT TACCTGGGTA CAGGTGATGC TTTTTCAGCC
    AGTCCTGATA TGGTAAAGCA AGGGCTTGTG GAAAATTATT TTGGCTGTCA AAGCAGTACG
    GATGTTTCTG ACACATGTGC TATTAGCTAC AGCCATTCAG TTAGCCCTCA GAAAGAAACT
    TCTGGAAAAG AAATGAGTAG TCCTCAGCGG GATCAGGGTA AAGATGAGGA AGAGGAAGAG
    CAGGATCAAC AAATGGTTCA AAACGGGTAT TATGAGGAGG CAGATTACTC CTCTCTGGAG
    GCCGCGGGAG ATGCTCACTG TGCAGATGGA GCTGACCTGG GAGGAGAAAG ACTTGCCAAG
    TCTGAAAGAC TGGCCGGCGA CTATCTGAGC GATGGCATAA CCATGCCTAC TGTCTGTACT
    TCTGGCTGTC TGTCCTTCCC GTCTGCACCA CGAGAGTCCC CTTGTGGCGT TAAGTACTCT
    TCTAAGAGTA AGTGTGACGC TGTTACCAAG CAGCCGAGCA GCGCCTCCCA CAGCTGCACG
    TCATCTCTGT CCTGGTACGA AAACTCGCCA AAGCCTCAGA TACAAGCCTT CCTTCAGGCA
    AAAGATGAAT TGAAGCAACT TAAACTCCCT GGGTTCATGT ACAGCGATGT TCCTCTGCTG
    GCATCCTCAG TCCCTTATTT TTGCGTGGAG GAGGAGGAGG AGGACCATTG TGGAAATGGA
    GTGCATCTCA TTGTCTGTGT GCATGGCCTA GACGGTAACA GTGCAGATCT CCGATTAGTA
    AAAACTTACA TTGAACTTGG GCTTCCTGGG GAAAGAATTG ATTTTCTTAT GTCTGAAAGA
    AATCAGAATG ATACTTTTGC TGATTTTGAT AGCATGACTG ATCGCCTTTT GGATGAGATA
    ATACAATATA TTCAGATATA TAGTCTAACA GTCTCAAAAA TAAGCTTTAT TGGACATTCT
    TTGGGCAATT TAATAATCCG TTCAGTGCTT ACAAGACCAC GGTTTAAATA TTACCTCAGC
    AGGCTTCATA CCTTTCTTTC ACTTTCTGGA CCTCACCTTG GTACACTCTA CAATAGCAGT
    GCTCTTGTTA ATACAGGTCT GTGGTTTATG CAGAAATGGA AAAAATCAGG TTCTCTTTTA
    CAATTGACAT GTCGTGATCA TTCAGACCCT CGCCAAACTT TCTTATACAA GCTTAGTAAT
    AAAGCAGGGC TTCACTACTT CAGAAATGTT GTCCTAGTGG GATCTCTGCA AGATCGCTAT
    GTTCCTTATC ATTCTGCCCG CATTGAAATG TGCAAGACAG CTTTGAAGGA CAAACAGTCA
    GGACAGATCT ATTCAGAAAT GATCCACAAC TTACTTCGCC CGGTTCTGCA AAGCAAGGAC
    TGTAACTTGG TTCGATATAA TGTCATCAAT GCGTTGCCCA ATACAGCTGA TTCACTCATT
    GGCAGAGCTG CACATATAGC TGTTCTGGAT TCAGAAATAT TTTTAGAGAA ATTCTTTCTG
    GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22441
    Clone ID Related Accession (Same CDS sequence) XM_002690004.4 , XM_015464710.1 , XM_002690004.5
    Accession Version XM_002690004.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104165.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC
    TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT
    AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA
    CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA
    AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA
    ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG
    GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC
    AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT
    CCAAAGACCT ACATGAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA
    GAAAGAACTG AACAAAAGTC TCCAGATATT GAAAATGTGC AACCAGACCT GTTTGATCCT
    TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGATATC
    TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA
    GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG
    CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA
    GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAATTT TGAGTCCTTA
    GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA
    GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT
    ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC
    ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAACCAAG CTCTGTTGCA
    GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT
    GAAAGGTTAT TTCCTCAGCT CTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC
    AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA
    AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA
    TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATGTGCCA
    AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA
    GGGCATTTGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT
    TTACAATTTG TTTTTTCAGA TGAAGATACT TCCAGTGATG TGAAAAGTAG TTGTAGCTCC
    AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT
    GTAGGAACCA CAGTTCCATT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT
    TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGGAAAAAC
    AGCGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAA AGATGAAACT
    TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG
    GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGTGC TATTAGCTAC
    AGCCATTCAG TTAGCCCTCA GAAAGAAACT TCTGGAAAAG AAATGAGTAG TCCTCAGCGG
    GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT
    TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGATGGA
    GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC
    GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA
    CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AGTGTGACGC TGTTACCAAG
    CAGCCGAGCA GCGCCTCCCA CAGCTGCACG TCATCTCTGT CCTGGTACGA AAACTCGCCA
    AAGCCTCAGA TACAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCT
    GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG
    GAGGAGGAGG AGGACCATTG TGGAAATGGA GTGCATCTCA TTGTCTGTGT GCATGGCCTA
    GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTTCCTGGG
    GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT
    AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA
    GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT
    ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA
    CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG
    CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT
    CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT
    GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG
    TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC
    TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT
    GCGTTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT
    TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690050.1
    CDS340..4896
    Translation

    Target ORF information:

    RefSeq Version XM_002690004.4
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690004.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC
    TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT
    AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA
    CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA
    AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA
    ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG
    GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC
    AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT
    CCAAAGACCT ACATGAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA
    GAAAGAACTG AACAAAAGTC TCCAGATATT GAAAATGTGC AACCAGACCT GTTTGATCCT
    TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGATATC
    TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA
    GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG
    CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA
    GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAATTT TGAGTCCTTA
    GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA
    GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT
    ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC
    ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAACCAAG CTCTGTTGCA
    GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT
    GAAAGGTTAT TTCCTCAGCT CTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC
    AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA
    AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA
    TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATGTGCCA
    AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA
    GGGCATTTGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT
    TTACAATTTG TTTTTTCAGA TGAAGATACT TCCAGTGATG TGAAAAGTAG TTGTAGCTCC
    AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT
    GTAGGAACCA CAGTTCCATT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT
    TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGGAAAAAC
    AGCGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAA AGATGAAACT
    TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG
    GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGTGC TATTAGCTAC
    AGCCATTCAG TTAGCCCTCA GAAAGAAACT TCTGGAAAAG AAATGAGTAG TCCTCAGCGG
    GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT
    TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGATGGA
    GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC
    GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA
    CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AGTGTGACGC TGTTACCAAG
    CAGCCGAGCA GCGCCTCCCA CAGCTGCACG TCATCTCTGT CCTGGTACGA AAACTCGCCA
    AAGCCTCAGA TACAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCT
    GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG
    GAGGAGGAGG AGGACCATTG TGGAAATGGA GTGCATCTCA TTGTCTGTGT GCATGGCCTA
    GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTTCCTGGG
    GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT
    AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA
    GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT
    ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA
    CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG
    CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT
    CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT
    GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG
    TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC
    TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT
    GCGTTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT
    TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22441
    Clone ID Related Accession (Same CDS sequence) XM_002690004.4 , XM_015464710.1 , XM_002690004.5
    Accession Version XM_015464710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645510.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC
    TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT
    AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA
    CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA
    AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA
    ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG
    GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC
    AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT
    CCAAAGACCT ACATGAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA
    GAAAGAACTG AACAAAAGTC TCCAGATATT GAAAATGTGC AACCAGACCT GTTTGATCCT
    TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGATATC
    TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA
    GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG
    CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA
    GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAATTT TGAGTCCTTA
    GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA
    GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT
    ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC
    ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAACCAAG CTCTGTTGCA
    GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT
    GAAAGGTTAT TTCCTCAGCT CTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC
    AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA
    AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA
    TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATGTGCCA
    AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA
    GGGCATTTGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT
    TTACAATTTG TTTTTTCAGA TGAAGATACT TCCAGTGATG TGAAAAGTAG TTGTAGCTCC
    AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT
    GTAGGAACCA CAGTTCCATT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT
    TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGGAAAAAC
    AGCGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAA AGATGAAACT
    TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG
    GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGTGC TATTAGCTAC
    AGCCATTCAG TTAGCCCTCA GAAAGAAACT TCTGGAAAAG AAATGAGTAG TCCTCAGCGG
    GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT
    TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGATGGA
    GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC
    GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA
    CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AGTGTGACGC TGTTACCAAG
    CAGCCGAGCA GCGCCTCCCA CAGCTGCACG TCATCTCTGT CCTGGTACGA AAACTCGCCA
    AAGCCTCAGA TACAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCT
    GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG
    GAGGAGGAGG AGGACCATTG TGGAAATGGA GTGCATCTCA TTGTCTGTGT GCATGGCCTA
    GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTTCCTGGG
    GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT
    AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA
    GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT
    ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA
    CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG
    CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT
    CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT
    GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG
    TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC
    TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT
    GCGTTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT
    TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320196.1
    CDS340..4896
    Translation

    Target ORF information:

    RefSeq Version XM_015464710.1
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC
    TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT
    AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA
    CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA
    AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA
    ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG
    GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC
    AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT
    CCAAAGACCT ACATGAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA
    GAAAGAACTG AACAAAAGTC TCCAGATATT GAAAATGTGC AACCAGACCT GTTTGATCCT
    TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGATATC
    TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA
    GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG
    CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA
    GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAATTT TGAGTCCTTA
    GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA
    GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT
    ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC
    ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAACCAAG CTCTGTTGCA
    GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT
    GAAAGGTTAT TTCCTCAGCT CTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC
    AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA
    AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA
    TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATGTGCCA
    AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA
    GGGCATTTGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT
    TTACAATTTG TTTTTTCAGA TGAAGATACT TCCAGTGATG TGAAAAGTAG TTGTAGCTCC
    AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT
    GTAGGAACCA CAGTTCCATT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT
    TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGGAAAAAC
    AGCGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAA AGATGAAACT
    TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG
    GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGTGC TATTAGCTAC
    AGCCATTCAG TTAGCCCTCA GAAAGAAACT TCTGGAAAAG AAATGAGTAG TCCTCAGCGG
    GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT
    TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGATGGA
    GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC
    GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA
    CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AGTGTGACGC TGTTACCAAG
    CAGCCGAGCA GCGCCTCCCA CAGCTGCACG TCATCTCTGT CCTGGTACGA AAACTCGCCA
    AAGCCTCAGA TACAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCT
    GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG
    GAGGAGGAGG AGGACCATTG TGGAAATGGA GTGCATCTCA TTGTCTGTGT GCATGGCCTA
    GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTTCCTGGG
    GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT
    AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA
    GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT
    ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA
    CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG
    CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT
    CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT
    GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG
    TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC
    TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT
    GCGTTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT
    TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22441
    Clone ID Related Accession (Same CDS sequence) XM_002690004.4 , XM_015464710.1 , XM_002690004.5
    Accession Version XM_002690004.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein FAM135A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690004.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC
    TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT
    AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA
    CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA
    AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA
    ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG
    GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC
    AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT
    CCAAAGACCT ACATGAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA
    GAAAGAACTG AACAAAAGTC TCCAGATATT GAAAATGTGC AACCAGACCT GTTTGATCCT
    TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGATATC
    TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA
    GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG
    CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA
    GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAATTT TGAGTCCTTA
    GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA
    GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT
    ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC
    ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAACCAAG CTCTGTTGCA
    GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT
    GAAAGGTTAT TTCCTCAGCT CTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC
    AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA
    AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA
    TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATGTGCCA
    AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA
    GGGCATTTGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT
    TTACAATTTG TTTTTTCAGA TGAAGATACT TCCAGTGATG TGAAAAGTAG TTGTAGCTCC
    AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT
    GTAGGAACCA CAGTTCCATT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT
    TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGGAAAAAC
    AGCGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAA AGATGAAACT
    TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG
    GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGTGC TATTAGCTAC
    AGCCATTCAG TTAGCCCTCA GAAAGAAACT TCTGGAAAAG AAATGAGTAG TCCTCAGCGG
    GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT
    TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGATGGA
    GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC
    GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA
    CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AGTGTGACGC TGTTACCAAG
    CAGCCGAGCA GCGCCTCCCA CAGCTGCACG TCATCTCTGT CCTGGTACGA AAACTCGCCA
    AAGCCTCAGA TACAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCT
    GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG
    GAGGAGGAGG AGGACCATTG TGGAAATGGA GTGCATCTCA TTGTCTGTGT GCATGGCCTA
    GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTTCCTGGG
    GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT
    AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA
    GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT
    ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA
    CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG
    CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT
    CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT
    GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG
    TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC
    TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT
    GCGTTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT
    TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690050.1
    CDS375..4931
    Translation

    Target ORF information:

    RefSeq Version XM_002690004.5
    Organism Bos taurus(cattle)
    Definition Bos taurus family with sequence similarity 135 member A (FAM135A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690004.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACTGAAG TTCAAGCAAT GGTAGAATTC TCCGTGGAGC TAAACAAGTT CTACAATGTG 
    GATTTATTTC AGAGAGGTTT TTACCAGATT CGTGCATCTA TGAAAATTCC ACCAAGAGTT
    CCTCACAGAG TAGAAGCTAG TTTGCTACAT GCAGCAGGTA TGACTTTAGC CTTTCCAGCT
    TCAGTTCATG ATTCTCTGAT TTGCAGTAAA ACATTTCAAA TTCTATATAA AAATGAGGAG
    GTTGTTTTAA ATGATGTCAT GATCTTCAAA GTTAAAATGT TGTTGGATGA AAAAAAGATT
    GAAGAAACCC TTGAGGAAAT GAATTTTCTG TTATCCCTGG ATCTACACTT CACAGATGGA
    GATTATTCGG CAGATGATCT GAATGCATTG CAGCTAATAA GCAGCCGCAC ACTGAAGCTG
    CACTTCAGCC TCCAGAGAGG CCTCCATCAC CACGTTAACG TCATGTTTGA TTATTTCCAC
    CTTTCTGTTG TGTCTGTTAC AGTCCATGCG TCACTGGTTG CGCTACACCA GCCACTGATA
    AGTTTTCCTC GTCCTGTGAA GACAACTTGG TTAAACAGAA ATGCACCAGC ACAAAACAAA
    GATTCTGTGA TTCCTACTCT TGAAAGTGTG GTCTTTGGTA TTAACTACAC AAAGCAGTTA
    TCACCAGACG GCTGCAGCTT TGTCATCGCA GATTCCTTCC TGCGTCACGC CTACCGTTTC
    CACTACACCC TCTGCGCTGC CCTGCTGCTG GCCTTCAAGG GGCTGCACAG CTACTTCATT
    ATGGTAACAG AAGAGCTCCC CTCTTGTCAG AAACTAGAAC TGGAGGAAAT GGATGTAGAA
    GCTCGACTTA CTGAATTATG TGAAGAAGTT AAGAAAATAG AAAATCCTGA TGAACTGGCA
    GAACTTATAA ATATGAATCT TGCACAACTT TGCTCACTTT TAATGGCTTT ATGGGGACAA
    TTTCTGGAAG TTATAACACT ACATGAGGAA CTAAGACTAT TACTAGCACA AGAGCACCAT
    ACTCTGAGGG TGCGCAGGTT TTCTGAGGCC TTCTTTTGTT TTGAACATCC AAGAGAAGCT
    GCCATTGCAT ACCAGGAACT TCATGCTCAG AGTCATCTCC AGATGTGCAC CGCTATCAAA
    AACACTTCCT TCTGCAGCTC TCTCCCACCC CTGCCTATTG AATGTAGTGA ATTAGATGGA
    GATCTCAATT CGTTACCTAT TATCTTTGAA GATAGGTATT TAGATTCAGT TACTGAAGAC
    TTAGATGCAC CCTGGATGGG AATTCAGAGT CTTCAGAGAT CAGAGTCCAG TAGAATGGAT
    AAATGTGAGA CTGAAGAAAG CACTCTAGCT GGACTTTCCA GCCCAGAGTT GAAAATCAGA
    CCTGCTGGGA CCTCCAGTTT TTGGTCTACA GAAGGTGAAA AGCAGCTAAC AAAGTCTCTA
    AAAGGAAAGA ATGAAGAATC AAATAAATCC AAGGTTAAGG TTACTAAGCT CATGAAAACA
    ATGAAACCTG AAAACACAAA AAAATTAATA AAACAGAACT CTAAGGATTT GGTGGTTTTG
    GTAGGCTACA AATGCTTGAA AAGTACAGCA TCAAATGATC TCTTTAAAAG CTTTGAAGGC
    AACCCTACAC ATAGTCAGAA GGAAGGTCTG GACCCCACAA TATATGGATA TAATTTTGAT
    CCAAAGACCT ACATGAGACA GACAAGTCAA AAGGAAGCTA GCTATTTGCC AGCTAATACA
    GAAAGAACTG AACAAAAGTC TCCAGATATT GAAAATGTGC AACCAGACCT GTTTGATCCT
    TTGAACTCTG GCAGCCTAAA TCTTTGTGCA AATTTGTCCA TTTCAGGTAA ACTTGATATC
    TCCCAGGATG ATAGTGAAAT TGCACAAGTG GAACACAGTG TGGCATCCAG AAGCTCATCA
    GACGATTGCC ATGATCGTCA GTCTGCCCCC ACTTCAGGAG TTAGGACAAT TGAAGTTAAG
    CCATGTAATA AAGACTGTTT CAGTGGAGAG AAAGTAACTG TTAAAATAGG ACCTTGGACA
    GAGCTTCAAC AAGATGAAAT ATTTGTGGAT AATTTACAAC TGCCCAATTT TGAGTCCTTA
    GACTCTAATG GTAAATCTAA ACCTATGGAA ATAACACTTG AAAAGGAAGC TTTGCAGGAA
    GCTAAGTGTC ATTCTGTCGG GGAATCGTTA GCTAAGTTAA GAAATAATCA ACCTGCTTCT
    ACAAGAGAAT ACCACCTTGT AGTGAGTGGA GACACCATTA AGTTACCAGA CATTAGTGCC
    ACGTGTGCCT CATCTCGGTT CTCAGACTCA GGTGTGGAAA GTGAACCAAG CTCTGTTGCA
    GCACATCCAA ACCCTGACAT AGTCTTTGAA ACTGTACCAG GGCAAGGTCT TTTCATCAGT
    GAAAGGTTAT TTCCTCAGCT CTTGATGAAG CCTGACTGTA ATGTAAAGTT TTCACTAGGC
    AGTCACTGTA CTGAGAGTAC AAGTGCTTTC AGTGAGATCC AGTCGTCCTT AACCTCCATA
    AATTCTCTGC CTTCTGATGA CGAGCTGTCA CCTGATGAAA ATCCTAAGAA ATCTGCTGTA
    TCTGAATGCC ACCTAAGTGA TAGCAAAACT GTGTTAAATC TAGGAACAAC TGATGTGCCA
    AAATGTGACG ATAGGAAAAA GTCAAGTATT ATTTTGCAGC AGCAGAGTGT CATATTTTCA
    GGGCATTTGG ACAATGAAAC TATAGAAATA CATTCCTTAA ATTCAAGCAC TAAAGACCCT
    TTACAATTTG TTTTTTCAGA TGAAGATACT TCCAGTGATG TGAAAAGTAG TTGTAGCTCC
    AAACCTAACT TGGACACTGT GTGTAAAGAC TCCCAGAGTC CTGATAAATC CAGTGACTCT
    GTAGGAACCA CAGTTCCATT AAGTTCAGAA CTGGCTTGTT CAGGCACCCC TTGTGTCGTT
    TCAGGTTCCG TTTCCACCAG GGCAGATAGC AGTGAAGATA GAACTGTGAA AAGGAAAAAC
    AGCGATGCAT TAAATCCCAA ACAAATGTGT TCAGAAGCCC CTGCAGTTAA AGATGAAACT
    TACCTGGGTA CAGGTGATGC TTTTTCAGCC AGTCCTGATA TGGTAAAGCA AGGGCTTGTG
    GAAAATTATT TTGGCTGTCA AAGCAGTACG GATGTTTCTG ACACATGTGC TATTAGCTAC
    AGCCATTCAG TTAGCCCTCA GAAAGAAACT TCTGGAAAAG AAATGAGTAG TCCTCAGCGG
    GATCAGGGTA AAGATGAGGA AGAGGAAGAG CAGGATCAAC AAATGGTTCA AAACGGGTAT
    TATGAGGAGG CAGATTACTC CTCTCTGGAG GCCGCGGGAG ATGCTCACTG TGCAGATGGA
    GCTGACCTGG GAGGAGAAAG ACTTGCCAAG TCTGAAAGAC TGGCCGGCGA CTATCTGAGC
    GATGGCATAA CCATGCCTAC TGTCTGTACT TCTGGCTGTC TGTCCTTCCC GTCTGCACCA
    CGAGAGTCCC CTTGTGGCGT TAAGTACTCT TCTAAGAGTA AGTGTGACGC TGTTACCAAG
    CAGCCGAGCA GCGCCTCCCA CAGCTGCACG TCATCTCTGT CCTGGTACGA AAACTCGCCA
    AAGCCTCAGA TACAAGCCTT CCTTCAGGCA AAAGATGAAT TGAAGCAACT TAAACTCCCT
    GGGTTCATGT ACAGCGATGT TCCTCTGCTG GCATCCTCAG TCCCTTATTT TTGCGTGGAG
    GAGGAGGAGG AGGACCATTG TGGAAATGGA GTGCATCTCA TTGTCTGTGT GCATGGCCTA
    GACGGTAACA GTGCAGATCT CCGATTAGTA AAAACTTACA TTGAACTTGG GCTTCCTGGG
    GAAAGAATTG ATTTTCTTAT GTCTGAAAGA AATCAGAATG ATACTTTTGC TGATTTTGAT
    AGCATGACTG ATCGCCTTTT GGATGAGATA ATACAATATA TTCAGATATA TAGTCTAACA
    GTCTCAAAAA TAAGCTTTAT TGGACATTCT TTGGGCAATT TAATAATCCG TTCAGTGCTT
    ACAAGACCAC GGTTTAAATA TTACCTCAGC AGGCTTCATA CCTTTCTTTC ACTTTCTGGA
    CCTCACCTTG GTACACTCTA CAATAGCAGT GCTCTTGTTA ATACAGGTCT GTGGTTTATG
    CAGAAATGGA AAAAATCAGG TTCTCTTTTA CAATTGACAT GTCGTGATCA TTCAGACCCT
    CGCCAAACTT TCTTATACAA GCTTAGTAAT AAAGCAGGGC TTCACTACTT CAGAAATGTT
    GTCCTAGTGG GATCTCTGCA AGATCGCTAT GTTCCTTATC ATTCTGCCCG CATTGAAATG
    TGCAAGACAG CTTTGAAGGA CAAACAGTCA GGACAGATCT ATTCAGAAAT GATCCACAAC
    TTACTTCGCC CGGTTCTGCA AAGCAAGGAC TGTAACTTGG TTCGATATAA TGTCATCAAT
    GCGTTGCCCA ATACAGCTGA TTCACTCATT GGCAGAGCTG CACATATAGC TGTTCTGGAT
    TCAGAAATAT TTTTAGAGAA ATTCTTTCTG GTTGCTGCCC TCAAATATTT CCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.