SPTBN5 cDNA ORF clone, Bos taurus(cattle)

The following SPTBN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa91675 XM_015473133.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91675 XM_015465045.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91676 XM_015465046.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91676 XM_015473134.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91677 XM_015473135.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91677 XM_015465047.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91678 XM_015473136.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91678 XM_015465048.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91679 XM_015473137.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91679 XM_015465049.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OBa91679 XM_015473137.1 Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa91675
    Clone ID Related Accession (Same CDS sequence) XM_015465045.1 , XM_015473133.1
    Accession Version XM_015465045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    601
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    1021
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    1141
    1201
    1261
    1321
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    1441
    1501
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    1621
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    1921
    1981
    2041
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    2641
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    5881
    5941
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    6301
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    6541
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    7141
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    9781
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    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG GTGGAACAGA AGCAGCAGGT GAAGGGGCTG
    AAACCCTTGA GAAGCTGGTG GCCCAGAGCA CAGCTGACTG AGACAGGGGA CCAGCAGCCA
    TCTGTCCCCA TTGAGTCTTG CATACGCTTC ACCGCCGTCT CCCCGGAGTG CACAGCGGGG
    ACCCTGGGGG ATGCAAAGGG TGTCCCCACC ACGGAGGGAA TTTTGGAACT TAAGCAGCAG
    CTCCTGCCTG GGAGAAGGCA GAACACGGCT TCTACAGCCT CCCTCGACCT CGTAGGAGTG
    CAGCGTGAGA AGCCGGGAGA TCACCATGAT GGAAAACACA CTTTTTCCTT AAGGCTAACT
    GCAGGAGAGA CCCTGTCTGC AGCGCCATCT GCAGGACAGA CTGGGAGCTG GCGGGGCACC
    CTGAGCAGCT GGACAGCCCC CAGTCTGAGC CCAGAGCTCA AAGCCAGACC CTCAGGCTTC
    CTGGCTGAGC GCAATGCTGA GAGGGTACCC GGCATGCCAG CTCCACTCAG ACTCCACAGC
    GGTACCCCGC CCGTCTGGCT GCAGGAGACA CAAGTGTGCA CCTATAGCTT CCCGCAGCCC
    GTGCGGCTCC CACAGACGGC TGGCCTCGCA GAGCAAACTG CAGAAGGCTC CCACCGACTG
    TCCTGCTCAG GCCAGATCCA TGGTCTGGGC CTCGAGGCAA AAGCAGAGCT GGCACCAAGC
    CTCAGGGCTA CAAGCGAGGA CACCCTGCTA TCTGGCCGTT CAGGACCAAC TTCTGGAACA
    GCTGGTTCCT CATCCAGGGA TGGGCCCCAC CGAGGTCAGA GCCTGCTCAA GTCACACCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320531.1
    CDS222..11084
    Translation

    Target ORF information:

    RefSeq Version XM_015465045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG GTGGAACAGA AGCAGCAGGT GAAGGGGCTG
    AAACCCTTGA GAAGCTGGTG GCCCAGAGCA CAGCTGACTG AGACAGGGGA CCAGCAGCCA
    TCTGTCCCCA TTGAGTCTTG CATACGCTTC ACCGCCGTCT CCCCGGAGTG CACAGCGGGG
    ACCCTGGGGG ATGCAAAGGG TGTCCCCACC ACGGAGGGAA TTTTGGAACT TAAGCAGCAG
    CTCCTGCCTG GGAGAAGGCA GAACACGGCT TCTACAGCCT CCCTCGACCT CGTAGGAGTG
    CAGCGTGAGA AGCCGGGAGA TCACCATGAT GGAAAACACA CTTTTTCCTT AAGGCTAACT
    GCAGGAGAGA CCCTGTCTGC AGCGCCATCT GCAGGACAGA CTGGGAGCTG GCGGGGCACC
    CTGAGCAGCT GGACAGCCCC CAGTCTGAGC CCAGAGCTCA AAGCCAGACC CTCAGGCTTC
    CTGGCTGAGC GCAATGCTGA GAGGGTACCC GGCATGCCAG CTCCACTCAG ACTCCACAGC
    GGTACCCCGC CCGTCTGGCT GCAGGAGACA CAAGTGTGCA CCTATAGCTT CCCGCAGCCC
    GTGCGGCTCC CACAGACGGC TGGCCTCGCA GAGCAAACTG CAGAAGGCTC CCACCGACTG
    TCCTGCTCAG GCCAGATCCA TGGTCTGGGC CTCGAGGCAA AAGCAGAGCT GGCACCAAGC
    CTCAGGGCTA CAAGCGAGGA CACCCTGCTA TCTGGCCGTT CAGGACCAAC TTCTGGAACA
    GCTGGTTCCT CATCCAGGGA TGGGCCCCAC CGAGGTCAGA GCCTGCTCAA GTCACACCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91675
    Clone ID Related Accession (Same CDS sequence) XM_015465045.1 , XM_015473133.1
    Accession Version XM_015473133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG GTGGAACAGA AGCAGCAGGT GAAGGGGCTG
    AAACCCTTGA GAAGCTGGTG GCCCAGAGCA CAGCTGACTG AGACAGGGGA CCAGCAGCCA
    TCTGTCCCCA TTGAGTCTTG CATACGCTTC ACCGCCGTCT CCCCGGAGTG CACAGCGGGG
    ACCCTGGGGG ATGCAAAGGG TGTCCCCACC ACGGAGGGAA TTTTGGAACT TAAGCAGCAG
    CTCCTGCCTG GGAGAAGGCA GAACACGGCT TCTACAGCCT CCCTCGACCT CGTAGGAGTG
    CAGCGTGAGA AGCCGGGAGA TCACCATGAT GGAAAACACA CTTTTTCCTT AAGGCTAACT
    GCAGGAGAGA CCCTGTCTGC AGCGCCATCT GCAGGACAGA CTGGGAGCTG GCGGGGCACC
    CTGAGCAGCT GGACAGCCCC CAGTCTGAGC CCAGAGCTCA AAGCCAGACC CTCAGGCTTC
    CTGGCTGAGC GCAATGCTGA GAGGGTACCC GGCATGCCAG CTCCACTCAG ACTCCACAGC
    GGTACCCCGC CCGTCTGGCT GCAGGAGACA CAAGTGTGCA CCTATAGCTT CCCGCAGCCC
    GTGCGGCTCC CACAGACGGC TGGCCTCGCA GAGCAAACTG CAGAAGGCTC CCACCGACTG
    TCCTGCTCAG GCCAGATCCA TGGTCTGGGC CTCGAGGCAA AAGCAGAGCT GGCACCAAGC
    CTCAGGGCTA CAAGCGAGGA CACCCTGCTA TCTGGCCGTT CAGGACCAAC TTCTGGAACA
    GCTGGTTCCT CATCCAGGGA TGGGCCCCAC CGAGGTCAGA GCCTGCTCAA GTCACACCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328619.1
    CDS222..11084
    Translation

    Target ORF information:

    RefSeq Version XM_015473133.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG GTGGAACAGA AGCAGCAGGT GAAGGGGCTG
    AAACCCTTGA GAAGCTGGTG GCCCAGAGCA CAGCTGACTG AGACAGGGGA CCAGCAGCCA
    TCTGTCCCCA TTGAGTCTTG CATACGCTTC ACCGCCGTCT CCCCGGAGTG CACAGCGGGG
    ACCCTGGGGG ATGCAAAGGG TGTCCCCACC ACGGAGGGAA TTTTGGAACT TAAGCAGCAG
    CTCCTGCCTG GGAGAAGGCA GAACACGGCT TCTACAGCCT CCCTCGACCT CGTAGGAGTG
    CAGCGTGAGA AGCCGGGAGA TCACCATGAT GGAAAACACA CTTTTTCCTT AAGGCTAACT
    GCAGGAGAGA CCCTGTCTGC AGCGCCATCT GCAGGACAGA CTGGGAGCTG GCGGGGCACC
    CTGAGCAGCT GGACAGCCCC CAGTCTGAGC CCAGAGCTCA AAGCCAGACC CTCAGGCTTC
    CTGGCTGAGC GCAATGCTGA GAGGGTACCC GGCATGCCAG CTCCACTCAG ACTCCACAGC
    GGTACCCCGC CCGTCTGGCT GCAGGAGACA CAAGTGTGCA CCTATAGCTT CCCGCAGCCC
    GTGCGGCTCC CACAGACGGC TGGCCTCGCA GAGCAAACTG CAGAAGGCTC CCACCGACTG
    TCCTGCTCAG GCCAGATCCA TGGTCTGGGC CTCGAGGCAA AAGCAGAGCT GGCACCAAGC
    CTCAGGGCTA CAAGCGAGGA CACCCTGCTA TCTGGCCGTT CAGGACCAAC TTCTGGAACA
    GCTGGTTCCT CATCCAGGGA TGGGCCCCAC CGAGGTCAGA GCCTGCTCAA GTCACACCCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91676
    Clone ID Related Accession (Same CDS sequence) XM_015473134.1 , XM_015465046.1
    Accession Version XM_015473134.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2521
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    2641
    2701
    2761
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    3001
    3061
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    3181
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
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    6121
    6181
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    6301
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    6421
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    7141
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    7501
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    7921
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    8401
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    8521
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    8641
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    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GCCCTGGAAG CAGAGCTCCA TCGCCACCAG
    GCCGTGTGTG CTGATCTCGT GCAGAGGGGA TGCAAACTGG GAGCCTGCGG GCCCCCAACG
    GGGCCGGATC CCTGGGAGCG GGCTGAGGTG GTGCAGGGCA TGTGGCAGCG GCTCTGGGCC
    CGGATGGATG AGCAGGGCAT GAGGCTGCAG GCGGCCCTGC TCGTCCAGCA GTACTTCGTG
    GACGTGGCGG AGGCAGACTC GTGGCTTCAG GAGCAGCGGT CTACACTGGA GAGTGCATCC
    TTTGGACAGG ACCAGGTGGC CACCGAGGCC CTACTGCGGC AACACCTGCA TCTGGAACGC
    TCTGTGCGCG CCTTCGGGAC CGAGCTGCGT CGATTAGATG AGCAGGCACA GGTGGCCACA
    GCCCAGGCAG CACTCCTGAG GGAGCCCCCA GAAGGCTGGA GCAGAACTGA TGGAGATGTC
    ACCAGGAGAC CTCAGGATGG GACCCAAATT TTAGCACTGC GGGGCAGCCC TCAGATCAAG
    ACAAGCTTCA CCTACAGAGG CACGCAATTC ACCCAGCTCC CAGGAGAGGC CCAGCAGCCC
    CCACACCAGA TGGACCCCAA CACCCCAGGA GGCTGCCAGG CCACAGTGGC CCTCAGGCAC
    ATCATGGGAC AGGAAGCTCA GGTGGTGTCT GCCCTGAGCT CTCCGAAGAT AGGCCCAAGA
    AACCCAGGGG CCTGGCGTGA GGTTTTCTGC CACTCTGGTC TGTGGCGCAC CCAGAAGATG
    ACCCTCCCAG ATGAGCCTGA CCCTGGCTTT GACCCCAACA CCATCCTCCA GACACAGGAC
    CGCTTGAACC AGGACTATGA GGGCCTGAGG GCCCAGGCAG AGCGCCGCAG GGCCCAGCTG
    GAGGAGGCCG TGGCCCTGTT TGACTTCTAT GGCTCATGTG GGGAGCTGCA GTCCTGGCTG
    ACCAAACAGA CAACGCTGTT GCAAATGCTG CAGCCCGAGG CCAACAACTT GGAAGTTATG
    CAGCTCAAGT ATGAGAACTT CCTCACTGCC CTGGCTGTGG GAAGAGGCCT CTGGACTGAG
    GTTAACAGCT CCGCTGAGCA GCTGAAGCAG AGGTGTCCTG GAGGTTCCCC CCAAATCCAG
    CTACAGCAGG AGGAACTGAG CCAGAGGTGG GAGCAGCTGG AGGCCCTGAA GAAAGAGAAG
    GAAACGCAGC TGGTGTGCAC AACGCACGTG TGCAGTTTTC TGCAGGAGTG TGGATCCACA
    CGGGTCCAGC TGCAAAACCT GGTACTCCAG CTAGAAAGCT TGGAGCTGGG GAACTCAGAG
    GATGGCCACC ATGTTCTGCG AGTGGGCCAG CAGAAGATGC TGGTGCTGGA GAAATACATT
    CGTCACCTGC AAAGGGCAGC TAGCAAGGTT GAGGAGATGG CCCCGGCCGA GAGCCCGTCC
    CTGCAGGCAC ATGTGGAGAC ACTGCAGGGG CTGCTGGAGC GAGTGCGGGA GCAAGTAGCC
    CAGCGGGCCA AGGCCCGGGC CCACCGGAGC TTCCTGCAGG AGAGCGAGCG GCTGCTGCTG
    TGTGCAGACA GTATCCAGGC CAAGCTCCAC AGCGAGGAGG AGGCGGTGGA TGTGGCCTCA
    GCCCAGCGGC TGCTGGGGAA GCACCGAGAC CTGCTGGAGG AGATCCACCT GCAGCAGGAG
    AGGCTGCAGC AGCTGGAGAC TCAGGGCCAG CCCATGGCAG CCTCGGGCTC CCCAGACTTC
    CGAGAGGCAG CTAGTACCCT GAGGCTCCTG GGCCACCAAG GCCAGGAGTT GAAGGCCGCC
    TGGGAGCAAA GACAGCAGCA ACTGCAGGAG GGGCTGGAAT TGCAGAAGTT TGGTCGCGAC
    GTGGATGGTT TCACTGCCTC CTGTGCCAAC CATGAGGCCT TCCTACAGCT GGACGTCCTC
    CAGGAGGGTG TGGTGGAGGC CCAGAGTCTG ATGCAGAAGC ACCAGGAGTG TGGGCGGCTG
    CTGGATGCCC TTGGCTCTAG AGCAGAGGAT CTACGGGCAC GTGGCAAGAA GCTGGCTCAG
    AGCCGACACC CCGCTGCACA CAAGGTCAGA GAACAACTGC AGAGCATCCA GGAGCAGTGG
    ACCAGGGTCC AGGAGAGGAG TGAACAGAGA AGGAGGCAGC TGCTGGCCTC CCTCCAGCTC
    CAGGAATGGA AGCAGGACGT GGTGGAGGTG ATGCTGTGGA TGGAGGAGAA GGGGCGGGTG
    TTGGTGGACG AGCCATCTCG AGAGCCCAGC AACATCATGA GGAAACTCAG GCGGCACGAG
    GCAGCCACGT GTGAGCTGGC AGCCACCCGA GGGCACGTGG AGGGCCTGCA GCAGGTCGGG
    AGAGAGCTGC TGAGCAGCAG GCCCCATGCC CAGGAGGACG TGCAGGCCAG GCTTCAGGGC
    CTAAGTAGCA AATGGGAAGA TTTGAGATGC AAGATGGCAG AGCGTGGGAA GCAGCTCCAG
    CAGGCTCGGC AGCAGGACCA GCTCCTGCGG CTGCTACAGG AAGCCAAGGA GAAGATGGAG
    CAGCTAGAGG GGACTGTGCA GAGGGCAGAG GTGGGACAGG ACCTGGGCTC CAGCCAGGGG
    CTGCAGAAAC AGCATTGCCA GCTGGAGGCC GAGAGCCAGG CACTGGCCAG CAAGATGGCT
    GCCCTTGTCC CCGAGGCCCG TCAGGCAATC AGTTCCCAGG CCATCATGGA GGAGACCCAA
    AAATACCTCC AGAGGTTCAA GGCCCTGCAG GGACATCTGG CCAGCCGGCG ACGGCAGCTG
    CAGGCCTCGG TTGAGCTGTA CCACTTCCAC CACCTGAGCA ACGCGGAGCT CATGTGGGTG
    GCAGAGCACA TGCCCACGCC CAGCGCCAGC TCTGCTAAGT GCCCAGATGG TGCCCACCGC
    CTTCTCCACA AGCACAAGGA ACTCCGGGTG CAGGTGAGAG CCCACCAGGG ACAGGTGCGA
    TGGGTGCTGG GCTCCGGACG ATACCTCGCA GCCTCCGGGC ACCCCCAGGC CCAGCACATC
    ATGGAGCGGT GCCAGGAGCT GGAAGGCCGC TGGGCGGATC TGGAGCAGGC GTGTGAGGCA
    CAGGCCCGGC ACCTGCAGCA GGCTGCTGCC CTCCAGGAGT GTTTCCTGGA CGCATCAGAG
    CTGGAGGACT GGGTGGAGGA GAAGTGGCCG CTGGTGAGCA GCCAGGACTA TGGTGGGGAT
    GAGACAGCCA CCATCAGGCT CATTAAGAAG CACCAGGCCC TGCAACAGGA ACTGGCTCGT
    TGTTGGAACT CCGTGGAGGA GCTTGACCGG AGAGCCCAAA CCCTCACTGG CCCCGAGGGC
    CCTGCGCAGC TGGGCGTGGT GCAGAAGAGG CTCCGGGAGC GGCTGCAGGC CCTGCAGGAG
    CTGGCAGCCA CACGGGACCG GGAGCTGGAG GGGACCCTGA AGCTGCATGA GTTCATGAGG
    GAGGCTGAGG ACCTGCAGGG CTGGCTGGCC AGCCAGAAGC AAGTGGCTGG AAGAGGAGAG
    AGCCTTGGGG AAGACTATGA GGACGTCCTG CATCTCTGCA CCAAGTTTTT CAGGTTTCAG
    CGCACAGTAG AGATGGGTGG CCAGCGGGTG GCAAGCTGCC AGCAGCTGGC AGAGAAGCTG
    CTGGAACGAG GGCACAGAGC AGCCCCCAGG GCCCATCAGA TGCAGCAGGA TCTGCAGGCT
    GCCTGGTCAG AGCTATGGGA GCTGACCCAG GCCCGAGGCC GCCTGCTCCG AGACGCTGAG
    GCCACCCTTA AGGTTCACCG AGACATGTTG GAAGCCCTCA CCCAGGTCCA GGAGAAAACC
    ACCAGCCTCC CCTGTGACGT GGCCCAGGAC CTGCGTGGGC TAGAGCCCCA GCTGAGGAGA
    CATGAGGGGC TGGAACGTGA ACTCATGGGC ATTGACCAGC AGCTGCAGGA GCTGCTGGAG
    ATGGGAGGCA CGGTGCAGAA GCTAGGGCCC GGGCCGCAGG CCCATGCAGT ACAGCAGAGG
    CAGCAGGCCC TGGTGCAGGC CTGGGAGACC CTGAAGCTGC GAGCAGAGCA GCGCCGGGCC
    CAGCTCGAGC GGGCATGGCT CCTGGCCCGC TTCCACATGG CGGTGCGGGA CTACACCTCC
    TGGGTGGCCA GCGTGTGGCA GGACCTGCAA GTGGAGGCAG GCTCCCAGGA GCCCGGGAGC
    GGCCCACTGA AGCTCAGCGC CCACCAACAG CTTCGGGCCA TGCTGGAGGC CCGGGAGGAG
    CTGTACCAGC AGGCCGCCCA GCTGGGCCAG CAGGCGCTTC TGGCTGCAGG GACATGCATC
    ACGGAGGTCC GGGACAGGCT GCAAGCCCTG CGGGACAAGC GGGAGCGGGT ATTCCAGGCC
    TGGGAGCAGA AGCAGGAGAG GCTGCAGGCC ATGGGCCAGG AGCAGCTCTT CCTCAGGAAG
    TGTGGCCGCC TGGACGAGAT CCTGAAGGCC CGGGAGGTCC TCCTGAAAAC CAGTGCCTTG
    GGGAGCTCAG TGGAAGAGGT AGAGCAGTTA ATCCGCAAGC ATGAGACCTT CCAGAAGGTG
    CTGGCTGCAC AGGAGGAGAA GGAGGCAGCC CTGTGTGAGC AAGTGAAGAT GCTCGGAGGC
    CCCAGGGCAC AGGATCTGCT TTGCACAGTG CTCGAGCGCC GGGCTCGGGT GAAGGAACTG
    GTGGAGAGTC GGCGACACGC CTTGCACACC TCCCTGATGG TGGCTGCCTT CATCAGGGCC
    GCCACCCAGG CCAAGGACTG GATCCAGGAG CAACTCCAGC AGCTGAAGGA GCCAGTCCCT
    TCTGGGGACC TGAAAGCTAA GTTGAGACAC CTGCAGAAAC ACCAGGCCTT TGAGGCTGAG
    GTCCAGGCCC ATGAGGAAAT CATAACCTCT GTTGTCAAGG AGGGCGAGGC TCTCCTAGCA
    CATAGCCACC CTCTGGTGGG AGAGGTCTCC CAGAGGCTGC GGGAGCTGCA GGAGCACTGG
    GAGAAGTTGA GGCAGGCAGT GGCTCTCCGG GGCGAGGACC TGGAGGACAA GCGGAACATC
    CTGGAGTTCC TGCAGAGAGT GGACACTGCA GAGGCCTGGA TCCAGGAGAT GGAGGTGATG
    GTGAATGTCA GCGACCTGGG CCAGGACCGT GAGCACTGCC AGCAGCTCCA CAGGCAGCTC
    CGCAAGCTCC GAGGAGTCTG GGCCGGGGAC ACGGTGGATG ATGCCCGCAT CAGAAGCATC
    AGTGACTTCT CACCACAGCT CAAGACTCAG GACACAGAGC AACTCAAGAC CATCAGGCAG
    CGGCAGCAGC AGCTCAACAA CAGGCCGGTC CCAGAGCAAG AAGCAGTGGG GCTGGGAGAG
    GGGGCTGAGG GTCCAACCCA GACTCAGCCT TACACCCTCT GCCTCAGGTG GAACAGTTTC
    CATGGCAACC TGCTCAGATA CCAGCAGCAG CTCGAAGTGG CCCTGGAAAT ACACACGTTG
    TCCCAAAAGC TTGATGACAT CACTCAGCAG GTCAGGAAGA AGGCCGCCCT GGTCCAGGCC
    CTGGACTGTG GGAAAGATCT GGACAATGTG CAGAGGCTGA AGCAGAAACA CAAGGAGCTG
    GAGCAGGAAA TGGGCCTCCT CCAGGCCCAG GTGGAGCCTC TAGATCGTGA AGCAGGCCGA
    GTCTGCCAGA GAAGCCCTGA GGTGGCCCAC GGCCTCAGCC ACCAGCAGCA GGAGATGATG
    GACAGCTGGC GGCAGCTCCA GAGCAGGATC CAGAAGTGGA AGGAGTCGCT GGACGCCTTG
    CAGCAAGCTC AGGAGCTGCA GGCAGTGCTT CGGGAACTGC TGGTCTGGGC CCAGAGGCTG
    CGAGCTCAGC TGGACTCCCG GAGAAGCCCC GGCAGCCTCG TGGAGGCCCA GCACATGTTG
    GAGGAACACC AGGAACTCAA AGCTGAGCTG GATTCCCGAA TGGACAGCAT CAGCCTGGCC
    CGGAGCACAG GGCAGCAACT GCTTGCTGCG GGGCACCCAT CCACCCCCAA CATTCGCCAG
    GCCCTGGCTG ATTTTAATCA GGAGCTGAAC AGCCTGGAAG GGGCCTGGCA TGAGCACCAG
    CTTCACCTGC ATCAGGCCCT GGAACTACAG CGGGTTCTGA GCTCTGTGGA GCAGATGGAA
    AGTTGGCTCT CCAGCCTGGA AGCCTGCCCA GCCAGTGAGG GTCTGGGGGA CTGCTTGGCC
    AACGTGGAGA CCCTGCTGTG GAAGCACAGG GTGCGTGAGC AGGACCTGGA GGCTCAGGCG
    GAGAAGATGA GCGCGCTGGA AGCCGCCAGC CGCAGCCTGC TCCAGGGCGG ACACCCCGAG
    GCCCAGGGCA CGCTGAGTCG GTGCCAGGCC ATGCTTCTGA GGAAAGAGGC ACTCCTGGAG
    CGAGCCAGGA CCCGTTGCTG CCAGCTGGAG GAGCTCAGGC AACTGCAGTC TTTCCTGCAG
    GACTCCTGTG AGATGGCCGC CTGGCTGAGG GAGAAGAACC TGGTGGCCCT GGAAGAGGGC
    TGGTGGGACC CAGTGGACCT GCAGGCCCAG TTGCAGCAAC AACAGCATCT CCAGGCGGAG
    CTGGACACCC GTGCACACCA CCAACAGAGG CTGCAGATGG AGGGGCAGAG GCTGCTACAG
    GATGGCCACT TGGCCTCAGA GACCATCCAG GAGCGGCTGC AGGAGCTGAG AAAGCTCTGG
    GAGGAGCTGC AGGCCCAGAG CCAGAGGAGG GCAGCCAAGC TACAGGAGGC CCGTGAGGCC
    CTGCGTCTGC GACGGAGCAT GGAGGAACTG GAGAGCTGGC TGGAGCCTGT GGAGCTCAAG
    CTGAGAGTCC CCATCGGGGG CCAGGACCAG CCTGGGCTGG ATGAGCTCCT GGGGGATCAG
    GGGGAGCTGG AGGCAGCAGT GGACAGGCAG GTCGGGCGGG CACAGGCCCT GCTGGGCCAA
    GCCCAGGCCC AGGCCTGTGT CCAGGAAGGT CACTGCCTGG CCCGAGACGT GGAAGAGCAG
    GCCCAGCGGC TGCTTCAGAG GTTCGAGAGC CTGCGGGCGC CCCTGCAGGA GCGCAGGACA
    GCCCTGGAGG CCCGGAGCCT GCTGCTGCAG TTCTTCAGGG ACGCAGAAGA GGAGATGGCC
    TGGGTGCAGG AGAAGCTGCT CCTGGTGGCC GCCCGGGACT GTGGCCAGAG CCCAAGTGCC
    CTGCGGCGCC TGCAGGAGAA GCACCAGAGC CTGGAGAGTG AGATGAGCAG CCACGAGGCT
    CTAACCCGGG CGGTGGTGGG CACAGGGCGC AAGCTGGTAC AGGCTGGGCA CTTCGCTGCC
    CGCGATGTGG CTGCCCGAGT GCAGCAGCTG GAGGACGCCA TGGGCCGCCT GCGGGCAGAG
    GCTGCACAGA GGCGTCGGCG GCTACAGCAG GCTCAGGAGG CCCAGTACTT CCTGACGGAG
    CTCCTGGAGG CAGAATCCTG GCTGGAAGAA CGGGGCTGTG GCCTGGACAT CGAGGATATG
    GGTCAGAGTG CCGAGGCCAC GCAGGCTTTC CTACGGCAGC TGGAGGCCAC CAGGAGGGAC
    CTGGAGGGAT TTTCCACGCG CATTGAGAGG CTGCAGCAGA CAGCAGCCCT CCTTGAGAGT
    GGGCAGAACC CAGAAAGCCC CAAGGTGCTT GCCCAGATGC ATGCGGTGAG GGACGCCCAC
    TCAGGGCTGC TGCAGAGAGT GGAGAGTAGG GGGCAAGGCC TGAAGGAGCA GCTGCAGCTC
    CACCAGCTGG AGCGGGAAGC CCTGCTCCTG GATGCCTGGC TGGCCAGCAA AGTGGCCACT
    GCCGAGTCCC AGGACTACGG GCAGGACCTA GAGGCTGTCA AGCTGCTGGA AGAGAAGTTC
    GATGCTTTCA GGAAGGATGT CCAGAGCCTG GGGCAGGCTA GGGTGCAGGC CCTGAGGGAG
    CGGGCAGGCT CCCTGGAACG AGCAGCACCC AGGTGCTCTC CTCAGATTCA AGCACAGAGG
    AGTCGCATTG AGGCGTCTTG GGAGAGGCTG GATCAGGCAG TGAAAGCCCG CACACAGAAC
    CTAGCTGCCG CCCGTGAGGT CCGAGGCTTG GAGCAGGCAG CAGCTAAGCT CCAGGCCCGG
    ATGCAAAAGA AGGCCACCCT GGTAGCTAGA GATGCCTTCA ACCTAAGCCT GTCATCAGTG
    CAGACTCTGC AGCAACAGCA CAGATGTCTG GAGAGGGAAC TGGCAGCGAT GGAAAAGGAG
    GTGGCACAGG TGCAGACGGA GGCCTGCCGC CTGGGCCAGC TCTACCCTGC AGCTCAGGAG
    GGCCTGGCCA AGCAGCTGGC CGGGGTGCAG GAGGCCTGGG CCACCCTGAA CGCAAAGGTC
    CATGAGCAAG ATCGGCAGCT GGAGAAGGCT GCCGAGGGCC ATGCCTTCCT CGGACGCTGT
    CGGGAATTGC TAGCATGGGC CACGGAGAAG CAGGCCCTGG TGTCCTCGGA GGAGCTGGCT
    GGGGATATAG CTCGGGCTGA GGGCCTCCTG GCACTGCATG AGAAGCTGGG GAGAGAAATC
    AAAGAGTACT GCCTTCAAGC CCAGAACGTA CAGCAGGAAG GGCAGCGGCT GGTGGACAGT
    GGCCACTGCA TGTCCCTGGA GGTAACTGGG TGTCTGCAGG ATCTGGACAG GCAGCTGCGG
    GCACTCAGAG AGGCCTGGGC CCTGCGCCGG GAACGCTGTG AGGAGAGCTG GCGTCTACAG
    AAGCTGCGGC AAGAGCTGGA CCAGGCCGAG GCCTGGCTGG CCTGCCGGGA GGGCCTCCTG
    CTGGACCCCA ACTGCGGGCA CTCCCTGTCT GATGTGGAGT TGCTGCTCTG CAGACACAAG
    GACTTAGAGA AGCTGCTGGC CTCCCAGGAA GAGAAGTTTG CCCGGCTGCA GCAGGAGGCA
    GAGGTGGAAC AGAAGCAGCA GGTGAAGGGG CTGAAACCCT TGAGAAGCTG GTGGCCCAGA
    GCACAGCTGA CTGAGACAGG GGACCAGCAG CCATCTGTCC CCATTGAGTC TTGCATACGC
    TTCACCGCCG TCTCCCCGGA GTGCACAGCG GGGACCCTGG GGGATGCAAA GGGTGTCCCC
    ACCACGGAGG GAATTTTGGA ACTTAAGCAG CAGCTCCTGC CTGGGAGAAG GCAGAACACG
    GCTTCTACAG CCTCCCTCGA CCTCGTAGGA GTGCAGCGTG AGAAGCCGGG AGATCACCAT
    GATGGAAAAC ACACTTTTTC CTTAAGGCTA ACTGCAGGAG AGACCCTGTC TGCAGCGCCA
    TCTGCAGGAC AGACTGGGAG CTGGCGGGGC ACCCTGAGCA GCTGGACAGC CCCCAGTCTG
    AGCCCAGAGC TCAAAGCCAG ACCCTCAGGC TTCCTGGCTG AGCGCAATGC TGAGAGGGTA
    CCCGGCATGC CAGCTCCACT CAGACTCCAC AGCGGTACCC CGCCCGTCTG GCTGCAGGAG
    ACACAAGTGT GCACCTATAG CTTCCCGCAG CCCGTGCGGC TCCCACAGAC GGCTGGCCTC
    GCAGAGCAAA CTGCAGAAGG CTCCCACCGA CTGTCCTGCT CAGGCCAGAT CCATGGTCTG
    GGCCTCGAGG CAAAAGCAGA GCTGGCACCA AGCCTCAGGG CTACAAGCGA GGACACCCTG
    CTATCTGGCC GTTCAGGACC AACTTCTGGA ACAGCTGGTT CCTCATCCAG GGATGGGCCC
    CACCGAGGTC AGAGCCTGCT CAAGTCACAC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328620.1
    CDS221..10996
    Translation

    Target ORF information:

    RefSeq Version XM_015473134.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GCCCTGGAAG CAGAGCTCCA TCGCCACCAG
    GCCGTGTGTG CTGATCTCGT GCAGAGGGGA TGCAAACTGG GAGCCTGCGG GCCCCCAACG
    GGGCCGGATC CCTGGGAGCG GGCTGAGGTG GTGCAGGGCA TGTGGCAGCG GCTCTGGGCC
    CGGATGGATG AGCAGGGCAT GAGGCTGCAG GCGGCCCTGC TCGTCCAGCA GTACTTCGTG
    GACGTGGCGG AGGCAGACTC GTGGCTTCAG GAGCAGCGGT CTACACTGGA GAGTGCATCC
    TTTGGACAGG ACCAGGTGGC CACCGAGGCC CTACTGCGGC AACACCTGCA TCTGGAACGC
    TCTGTGCGCG CCTTCGGGAC CGAGCTGCGT CGATTAGATG AGCAGGCACA GGTGGCCACA
    GCCCAGGCAG CACTCCTGAG GGAGCCCCCA GAAGGCTGGA GCAGAACTGA TGGAGATGTC
    ACCAGGAGAC CTCAGGATGG GACCCAAATT TTAGCACTGC GGGGCAGCCC TCAGATCAAG
    ACAAGCTTCA CCTACAGAGG CACGCAATTC ACCCAGCTCC CAGGAGAGGC CCAGCAGCCC
    CCACACCAGA TGGACCCCAA CACCCCAGGA GGCTGCCAGG CCACAGTGGC CCTCAGGCAC
    ATCATGGGAC AGGAAGCTCA GGTGGTGTCT GCCCTGAGCT CTCCGAAGAT AGGCCCAAGA
    AACCCAGGGG CCTGGCGTGA GGTTTTCTGC CACTCTGGTC TGTGGCGCAC CCAGAAGATG
    ACCCTCCCAG ATGAGCCTGA CCCTGGCTTT GACCCCAACA CCATCCTCCA GACACAGGAC
    CGCTTGAACC AGGACTATGA GGGCCTGAGG GCCCAGGCAG AGCGCCGCAG GGCCCAGCTG
    GAGGAGGCCG TGGCCCTGTT TGACTTCTAT GGCTCATGTG GGGAGCTGCA GTCCTGGCTG
    ACCAAACAGA CAACGCTGTT GCAAATGCTG CAGCCCGAGG CCAACAACTT GGAAGTTATG
    CAGCTCAAGT ATGAGAACTT CCTCACTGCC CTGGCTGTGG GAAGAGGCCT CTGGACTGAG
    GTTAACAGCT CCGCTGAGCA GCTGAAGCAG AGGTGTCCTG GAGGTTCCCC CCAAATCCAG
    CTACAGCAGG AGGAACTGAG CCAGAGGTGG GAGCAGCTGG AGGCCCTGAA GAAAGAGAAG
    GAAACGCAGC TGGTGTGCAC AACGCACGTG TGCAGTTTTC TGCAGGAGTG TGGATCCACA
    CGGGTCCAGC TGCAAAACCT GGTACTCCAG CTAGAAAGCT TGGAGCTGGG GAACTCAGAG
    GATGGCCACC ATGTTCTGCG AGTGGGCCAG CAGAAGATGC TGGTGCTGGA GAAATACATT
    CGTCACCTGC AAAGGGCAGC TAGCAAGGTT GAGGAGATGG CCCCGGCCGA GAGCCCGTCC
    CTGCAGGCAC ATGTGGAGAC ACTGCAGGGG CTGCTGGAGC GAGTGCGGGA GCAAGTAGCC
    CAGCGGGCCA AGGCCCGGGC CCACCGGAGC TTCCTGCAGG AGAGCGAGCG GCTGCTGCTG
    TGTGCAGACA GTATCCAGGC CAAGCTCCAC AGCGAGGAGG AGGCGGTGGA TGTGGCCTCA
    GCCCAGCGGC TGCTGGGGAA GCACCGAGAC CTGCTGGAGG AGATCCACCT GCAGCAGGAG
    AGGCTGCAGC AGCTGGAGAC TCAGGGCCAG CCCATGGCAG CCTCGGGCTC CCCAGACTTC
    CGAGAGGCAG CTAGTACCCT GAGGCTCCTG GGCCACCAAG GCCAGGAGTT GAAGGCCGCC
    TGGGAGCAAA GACAGCAGCA ACTGCAGGAG GGGCTGGAAT TGCAGAAGTT TGGTCGCGAC
    GTGGATGGTT TCACTGCCTC CTGTGCCAAC CATGAGGCCT TCCTACAGCT GGACGTCCTC
    CAGGAGGGTG TGGTGGAGGC CCAGAGTCTG ATGCAGAAGC ACCAGGAGTG TGGGCGGCTG
    CTGGATGCCC TTGGCTCTAG AGCAGAGGAT CTACGGGCAC GTGGCAAGAA GCTGGCTCAG
    AGCCGACACC CCGCTGCACA CAAGGTCAGA GAACAACTGC AGAGCATCCA GGAGCAGTGG
    ACCAGGGTCC AGGAGAGGAG TGAACAGAGA AGGAGGCAGC TGCTGGCCTC CCTCCAGCTC
    CAGGAATGGA AGCAGGACGT GGTGGAGGTG ATGCTGTGGA TGGAGGAGAA GGGGCGGGTG
    TTGGTGGACG AGCCATCTCG AGAGCCCAGC AACATCATGA GGAAACTCAG GCGGCACGAG
    GCAGCCACGT GTGAGCTGGC AGCCACCCGA GGGCACGTGG AGGGCCTGCA GCAGGTCGGG
    AGAGAGCTGC TGAGCAGCAG GCCCCATGCC CAGGAGGACG TGCAGGCCAG GCTTCAGGGC
    CTAAGTAGCA AATGGGAAGA TTTGAGATGC AAGATGGCAG AGCGTGGGAA GCAGCTCCAG
    CAGGCTCGGC AGCAGGACCA GCTCCTGCGG CTGCTACAGG AAGCCAAGGA GAAGATGGAG
    CAGCTAGAGG GGACTGTGCA GAGGGCAGAG GTGGGACAGG ACCTGGGCTC CAGCCAGGGG
    CTGCAGAAAC AGCATTGCCA GCTGGAGGCC GAGAGCCAGG CACTGGCCAG CAAGATGGCT
    GCCCTTGTCC CCGAGGCCCG TCAGGCAATC AGTTCCCAGG CCATCATGGA GGAGACCCAA
    AAATACCTCC AGAGGTTCAA GGCCCTGCAG GGACATCTGG CCAGCCGGCG ACGGCAGCTG
    CAGGCCTCGG TTGAGCTGTA CCACTTCCAC CACCTGAGCA ACGCGGAGCT CATGTGGGTG
    GCAGAGCACA TGCCCACGCC CAGCGCCAGC TCTGCTAAGT GCCCAGATGG TGCCCACCGC
    CTTCTCCACA AGCACAAGGA ACTCCGGGTG CAGGTGAGAG CCCACCAGGG ACAGGTGCGA
    TGGGTGCTGG GCTCCGGACG ATACCTCGCA GCCTCCGGGC ACCCCCAGGC CCAGCACATC
    ATGGAGCGGT GCCAGGAGCT GGAAGGCCGC TGGGCGGATC TGGAGCAGGC GTGTGAGGCA
    CAGGCCCGGC ACCTGCAGCA GGCTGCTGCC CTCCAGGAGT GTTTCCTGGA CGCATCAGAG
    CTGGAGGACT GGGTGGAGGA GAAGTGGCCG CTGGTGAGCA GCCAGGACTA TGGTGGGGAT
    GAGACAGCCA CCATCAGGCT CATTAAGAAG CACCAGGCCC TGCAACAGGA ACTGGCTCGT
    TGTTGGAACT CCGTGGAGGA GCTTGACCGG AGAGCCCAAA CCCTCACTGG CCCCGAGGGC
    CCTGCGCAGC TGGGCGTGGT GCAGAAGAGG CTCCGGGAGC GGCTGCAGGC CCTGCAGGAG
    CTGGCAGCCA CACGGGACCG GGAGCTGGAG GGGACCCTGA AGCTGCATGA GTTCATGAGG
    GAGGCTGAGG ACCTGCAGGG CTGGCTGGCC AGCCAGAAGC AAGTGGCTGG AAGAGGAGAG
    AGCCTTGGGG AAGACTATGA GGACGTCCTG CATCTCTGCA CCAAGTTTTT CAGGTTTCAG
    CGCACAGTAG AGATGGGTGG CCAGCGGGTG GCAAGCTGCC AGCAGCTGGC AGAGAAGCTG
    CTGGAACGAG GGCACAGAGC AGCCCCCAGG GCCCATCAGA TGCAGCAGGA TCTGCAGGCT
    GCCTGGTCAG AGCTATGGGA GCTGACCCAG GCCCGAGGCC GCCTGCTCCG AGACGCTGAG
    GCCACCCTTA AGGTTCACCG AGACATGTTG GAAGCCCTCA CCCAGGTCCA GGAGAAAACC
    ACCAGCCTCC CCTGTGACGT GGCCCAGGAC CTGCGTGGGC TAGAGCCCCA GCTGAGGAGA
    CATGAGGGGC TGGAACGTGA ACTCATGGGC ATTGACCAGC AGCTGCAGGA GCTGCTGGAG
    ATGGGAGGCA CGGTGCAGAA GCTAGGGCCC GGGCCGCAGG CCCATGCAGT ACAGCAGAGG
    CAGCAGGCCC TGGTGCAGGC CTGGGAGACC CTGAAGCTGC GAGCAGAGCA GCGCCGGGCC
    CAGCTCGAGC GGGCATGGCT CCTGGCCCGC TTCCACATGG CGGTGCGGGA CTACACCTCC
    TGGGTGGCCA GCGTGTGGCA GGACCTGCAA GTGGAGGCAG GCTCCCAGGA GCCCGGGAGC
    GGCCCACTGA AGCTCAGCGC CCACCAACAG CTTCGGGCCA TGCTGGAGGC CCGGGAGGAG
    CTGTACCAGC AGGCCGCCCA GCTGGGCCAG CAGGCGCTTC TGGCTGCAGG GACATGCATC
    ACGGAGGTCC GGGACAGGCT GCAAGCCCTG CGGGACAAGC GGGAGCGGGT ATTCCAGGCC
    TGGGAGCAGA AGCAGGAGAG GCTGCAGGCC ATGGGCCAGG AGCAGCTCTT CCTCAGGAAG
    TGTGGCCGCC TGGACGAGAT CCTGAAGGCC CGGGAGGTCC TCCTGAAAAC CAGTGCCTTG
    GGGAGCTCAG TGGAAGAGGT AGAGCAGTTA ATCCGCAAGC ATGAGACCTT CCAGAAGGTG
    CTGGCTGCAC AGGAGGAGAA GGAGGCAGCC CTGTGTGAGC AAGTGAAGAT GCTCGGAGGC
    CCCAGGGCAC AGGATCTGCT TTGCACAGTG CTCGAGCGCC GGGCTCGGGT GAAGGAACTG
    GTGGAGAGTC GGCGACACGC CTTGCACACC TCCCTGATGG TGGCTGCCTT CATCAGGGCC
    GCCACCCAGG CCAAGGACTG GATCCAGGAG CAACTCCAGC AGCTGAAGGA GCCAGTCCCT
    TCTGGGGACC TGAAAGCTAA GTTGAGACAC CTGCAGAAAC ACCAGGCCTT TGAGGCTGAG
    GTCCAGGCCC ATGAGGAAAT CATAACCTCT GTTGTCAAGG AGGGCGAGGC TCTCCTAGCA
    CATAGCCACC CTCTGGTGGG AGAGGTCTCC CAGAGGCTGC GGGAGCTGCA GGAGCACTGG
    GAGAAGTTGA GGCAGGCAGT GGCTCTCCGG GGCGAGGACC TGGAGGACAA GCGGAACATC
    CTGGAGTTCC TGCAGAGAGT GGACACTGCA GAGGCCTGGA TCCAGGAGAT GGAGGTGATG
    GTGAATGTCA GCGACCTGGG CCAGGACCGT GAGCACTGCC AGCAGCTCCA CAGGCAGCTC
    CGCAAGCTCC GAGGAGTCTG GGCCGGGGAC ACGGTGGATG ATGCCCGCAT CAGAAGCATC
    AGTGACTTCT CACCACAGCT CAAGACTCAG GACACAGAGC AACTCAAGAC CATCAGGCAG
    CGGCAGCAGC AGCTCAACAA CAGGCCGGTC CCAGAGCAAG AAGCAGTGGG GCTGGGAGAG
    GGGGCTGAGG GTCCAACCCA GACTCAGCCT TACACCCTCT GCCTCAGGTG GAACAGTTTC
    CATGGCAACC TGCTCAGATA CCAGCAGCAG CTCGAAGTGG CCCTGGAAAT ACACACGTTG
    TCCCAAAAGC TTGATGACAT CACTCAGCAG GTCAGGAAGA AGGCCGCCCT GGTCCAGGCC
    CTGGACTGTG GGAAAGATCT GGACAATGTG CAGAGGCTGA AGCAGAAACA CAAGGAGCTG
    GAGCAGGAAA TGGGCCTCCT CCAGGCCCAG GTGGAGCCTC TAGATCGTGA AGCAGGCCGA
    GTCTGCCAGA GAAGCCCTGA GGTGGCCCAC GGCCTCAGCC ACCAGCAGCA GGAGATGATG
    GACAGCTGGC GGCAGCTCCA GAGCAGGATC CAGAAGTGGA AGGAGTCGCT GGACGCCTTG
    CAGCAAGCTC AGGAGCTGCA GGCAGTGCTT CGGGAACTGC TGGTCTGGGC CCAGAGGCTG
    CGAGCTCAGC TGGACTCCCG GAGAAGCCCC GGCAGCCTCG TGGAGGCCCA GCACATGTTG
    GAGGAACACC AGGAACTCAA AGCTGAGCTG GATTCCCGAA TGGACAGCAT CAGCCTGGCC
    CGGAGCACAG GGCAGCAACT GCTTGCTGCG GGGCACCCAT CCACCCCCAA CATTCGCCAG
    GCCCTGGCTG ATTTTAATCA GGAGCTGAAC AGCCTGGAAG GGGCCTGGCA TGAGCACCAG
    CTTCACCTGC ATCAGGCCCT GGAACTACAG CGGGTTCTGA GCTCTGTGGA GCAGATGGAA
    AGTTGGCTCT CCAGCCTGGA AGCCTGCCCA GCCAGTGAGG GTCTGGGGGA CTGCTTGGCC
    AACGTGGAGA CCCTGCTGTG GAAGCACAGG GTGCGTGAGC AGGACCTGGA GGCTCAGGCG
    GAGAAGATGA GCGCGCTGGA AGCCGCCAGC CGCAGCCTGC TCCAGGGCGG ACACCCCGAG
    GCCCAGGGCA CGCTGAGTCG GTGCCAGGCC ATGCTTCTGA GGAAAGAGGC ACTCCTGGAG
    CGAGCCAGGA CCCGTTGCTG CCAGCTGGAG GAGCTCAGGC AACTGCAGTC TTTCCTGCAG
    GACTCCTGTG AGATGGCCGC CTGGCTGAGG GAGAAGAACC TGGTGGCCCT GGAAGAGGGC
    TGGTGGGACC CAGTGGACCT GCAGGCCCAG TTGCAGCAAC AACAGCATCT CCAGGCGGAG
    CTGGACACCC GTGCACACCA CCAACAGAGG CTGCAGATGG AGGGGCAGAG GCTGCTACAG
    GATGGCCACT TGGCCTCAGA GACCATCCAG GAGCGGCTGC AGGAGCTGAG AAAGCTCTGG
    GAGGAGCTGC AGGCCCAGAG CCAGAGGAGG GCAGCCAAGC TACAGGAGGC CCGTGAGGCC
    CTGCGTCTGC GACGGAGCAT GGAGGAACTG GAGAGCTGGC TGGAGCCTGT GGAGCTCAAG
    CTGAGAGTCC CCATCGGGGG CCAGGACCAG CCTGGGCTGG ATGAGCTCCT GGGGGATCAG
    GGGGAGCTGG AGGCAGCAGT GGACAGGCAG GTCGGGCGGG CACAGGCCCT GCTGGGCCAA
    GCCCAGGCCC AGGCCTGTGT CCAGGAAGGT CACTGCCTGG CCCGAGACGT GGAAGAGCAG
    GCCCAGCGGC TGCTTCAGAG GTTCGAGAGC CTGCGGGCGC CCCTGCAGGA GCGCAGGACA
    GCCCTGGAGG CCCGGAGCCT GCTGCTGCAG TTCTTCAGGG ACGCAGAAGA GGAGATGGCC
    TGGGTGCAGG AGAAGCTGCT CCTGGTGGCC GCCCGGGACT GTGGCCAGAG CCCAAGTGCC
    CTGCGGCGCC TGCAGGAGAA GCACCAGAGC CTGGAGAGTG AGATGAGCAG CCACGAGGCT
    CTAACCCGGG CGGTGGTGGG CACAGGGCGC AAGCTGGTAC AGGCTGGGCA CTTCGCTGCC
    CGCGATGTGG CTGCCCGAGT GCAGCAGCTG GAGGACGCCA TGGGCCGCCT GCGGGCAGAG
    GCTGCACAGA GGCGTCGGCG GCTACAGCAG GCTCAGGAGG CCCAGTACTT CCTGACGGAG
    CTCCTGGAGG CAGAATCCTG GCTGGAAGAA CGGGGCTGTG GCCTGGACAT CGAGGATATG
    GGTCAGAGTG CCGAGGCCAC GCAGGCTTTC CTACGGCAGC TGGAGGCCAC CAGGAGGGAC
    CTGGAGGGAT TTTCCACGCG CATTGAGAGG CTGCAGCAGA CAGCAGCCCT CCTTGAGAGT
    GGGCAGAACC CAGAAAGCCC CAAGGTGCTT GCCCAGATGC ATGCGGTGAG GGACGCCCAC
    TCAGGGCTGC TGCAGAGAGT GGAGAGTAGG GGGCAAGGCC TGAAGGAGCA GCTGCAGCTC
    CACCAGCTGG AGCGGGAAGC CCTGCTCCTG GATGCCTGGC TGGCCAGCAA AGTGGCCACT
    GCCGAGTCCC AGGACTACGG GCAGGACCTA GAGGCTGTCA AGCTGCTGGA AGAGAAGTTC
    GATGCTTTCA GGAAGGATGT CCAGAGCCTG GGGCAGGCTA GGGTGCAGGC CCTGAGGGAG
    CGGGCAGGCT CCCTGGAACG AGCAGCACCC AGGTGCTCTC CTCAGATTCA AGCACAGAGG
    AGTCGCATTG AGGCGTCTTG GGAGAGGCTG GATCAGGCAG TGAAAGCCCG CACACAGAAC
    CTAGCTGCCG CCCGTGAGGT CCGAGGCTTG GAGCAGGCAG CAGCTAAGCT CCAGGCCCGG
    ATGCAAAAGA AGGCCACCCT GGTAGCTAGA GATGCCTTCA ACCTAAGCCT GTCATCAGTG
    CAGACTCTGC AGCAACAGCA CAGATGTCTG GAGAGGGAAC TGGCAGCGAT GGAAAAGGAG
    GTGGCACAGG TGCAGACGGA GGCCTGCCGC CTGGGCCAGC TCTACCCTGC AGCTCAGGAG
    GGCCTGGCCA AGCAGCTGGC CGGGGTGCAG GAGGCCTGGG CCACCCTGAA CGCAAAGGTC
    CATGAGCAAG ATCGGCAGCT GGAGAAGGCT GCCGAGGGCC ATGCCTTCCT CGGACGCTGT
    CGGGAATTGC TAGCATGGGC CACGGAGAAG CAGGCCCTGG TGTCCTCGGA GGAGCTGGCT
    GGGGATATAG CTCGGGCTGA GGGCCTCCTG GCACTGCATG AGAAGCTGGG GAGAGAAATC
    AAAGAGTACT GCCTTCAAGC CCAGAACGTA CAGCAGGAAG GGCAGCGGCT GGTGGACAGT
    GGCCACTGCA TGTCCCTGGA GGTAACTGGG TGTCTGCAGG ATCTGGACAG GCAGCTGCGG
    GCACTCAGAG AGGCCTGGGC CCTGCGCCGG GAACGCTGTG AGGAGAGCTG GCGTCTACAG
    AAGCTGCGGC AAGAGCTGGA CCAGGCCGAG GCCTGGCTGG CCTGCCGGGA GGGCCTCCTG
    CTGGACCCCA ACTGCGGGCA CTCCCTGTCT GATGTGGAGT TGCTGCTCTG CAGACACAAG
    GACTTAGAGA AGCTGCTGGC CTCCCAGGAA GAGAAGTTTG CCCGGCTGCA GCAGGAGGCA
    GAGGTGGAAC AGAAGCAGCA GGTGAAGGGG CTGAAACCCT TGAGAAGCTG GTGGCCCAGA
    GCACAGCTGA CTGAGACAGG GGACCAGCAG CCATCTGTCC CCATTGAGTC TTGCATACGC
    TTCACCGCCG TCTCCCCGGA GTGCACAGCG GGGACCCTGG GGGATGCAAA GGGTGTCCCC
    ACCACGGAGG GAATTTTGGA ACTTAAGCAG CAGCTCCTGC CTGGGAGAAG GCAGAACACG
    GCTTCTACAG CCTCCCTCGA CCTCGTAGGA GTGCAGCGTG AGAAGCCGGG AGATCACCAT
    GATGGAAAAC ACACTTTTTC CTTAAGGCTA ACTGCAGGAG AGACCCTGTC TGCAGCGCCA
    TCTGCAGGAC AGACTGGGAG CTGGCGGGGC ACCCTGAGCA GCTGGACAGC CCCCAGTCTG
    AGCCCAGAGC TCAAAGCCAG ACCCTCAGGC TTCCTGGCTG AGCGCAATGC TGAGAGGGTA
    CCCGGCATGC CAGCTCCACT CAGACTCCAC AGCGGTACCC CGCCCGTCTG GCTGCAGGAG
    ACACAAGTGT GCACCTATAG CTTCCCGCAG CCCGTGCGGC TCCCACAGAC GGCTGGCCTC
    GCAGAGCAAA CTGCAGAAGG CTCCCACCGA CTGTCCTGCT CAGGCCAGAT CCATGGTCTG
    GGCCTCGAGG CAAAAGCAGA GCTGGCACCA AGCCTCAGGG CTACAAGCGA GGACACCCTG
    CTATCTGGCC GTTCAGGACC AACTTCTGGA ACAGCTGGTT CCTCATCCAG GGATGGGCCC
    CACCGAGGTC AGAGCCTGCT CAAGTCACAC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91676
    Clone ID Related Accession (Same CDS sequence) XM_015473134.1 , XM_015465046.1
    Accession Version XM_015465046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GCCCTGGAAG CAGAGCTCCA TCGCCACCAG
    GCCGTGTGTG CTGATCTCGT GCAGAGGGGA TGCAAACTGG GAGCCTGCGG GCCCCCAACG
    GGGCCGGATC CCTGGGAGCG GGCTGAGGTG GTGCAGGGCA TGTGGCAGCG GCTCTGGGCC
    CGGATGGATG AGCAGGGCAT GAGGCTGCAG GCGGCCCTGC TCGTCCAGCA GTACTTCGTG
    GACGTGGCGG AGGCAGACTC GTGGCTTCAG GAGCAGCGGT CTACACTGGA GAGTGCATCC
    TTTGGACAGG ACCAGGTGGC CACCGAGGCC CTACTGCGGC AACACCTGCA TCTGGAACGC
    TCTGTGCGCG CCTTCGGGAC CGAGCTGCGT CGATTAGATG AGCAGGCACA GGTGGCCACA
    GCCCAGGCAG CACTCCTGAG GGAGCCCCCA GAAGGCTGGA GCAGAACTGA TGGAGATGTC
    ACCAGGAGAC CTCAGGATGG GACCCAAATT TTAGCACTGC GGGGCAGCCC TCAGATCAAG
    ACAAGCTTCA CCTACAGAGG CACGCAATTC ACCCAGCTCC CAGGAGAGGC CCAGCAGCCC
    CCACACCAGA TGGACCCCAA CACCCCAGGA GGCTGCCAGG CCACAGTGGC CCTCAGGCAC
    ATCATGGGAC AGGAAGCTCA GGTGGTGTCT GCCCTGAGCT CTCCGAAGAT AGGCCCAAGA
    AACCCAGGGG CCTGGCGTGA GGTTTTCTGC CACTCTGGTC TGTGGCGCAC CCAGAAGATG
    ACCCTCCCAG ATGAGCCTGA CCCTGGCTTT GACCCCAACA CCATCCTCCA GACACAGGAC
    CGCTTGAACC AGGACTATGA GGGCCTGAGG GCCCAGGCAG AGCGCCGCAG GGCCCAGCTG
    GAGGAGGCCG TGGCCCTGTT TGACTTCTAT GGCTCATGTG GGGAGCTGCA GTCCTGGCTG
    ACCAAACAGA CAACGCTGTT GCAAATGCTG CAGCCCGAGG CCAACAACTT GGAAGTTATG
    CAGCTCAAGT ATGAGAACTT CCTCACTGCC CTGGCTGTGG GAAGAGGCCT CTGGACTGAG
    GTTAACAGCT CCGCTGAGCA GCTGAAGCAG AGGTGTCCTG GAGGTTCCCC CCAAATCCAG
    CTACAGCAGG AGGAACTGAG CCAGAGGTGG GAGCAGCTGG AGGCCCTGAA GAAAGAGAAG
    GAAACGCAGC TGGTGTGCAC AACGCACGTG TGCAGTTTTC TGCAGGAGTG TGGATCCACA
    CGGGTCCAGC TGCAAAACCT GGTACTCCAG CTAGAAAGCT TGGAGCTGGG GAACTCAGAG
    GATGGCCACC ATGTTCTGCG AGTGGGCCAG CAGAAGATGC TGGTGCTGGA GAAATACATT
    CGTCACCTGC AAAGGGCAGC TAGCAAGGTT GAGGAGATGG CCCCGGCCGA GAGCCCGTCC
    CTGCAGGCAC ATGTGGAGAC ACTGCAGGGG CTGCTGGAGC GAGTGCGGGA GCAAGTAGCC
    CAGCGGGCCA AGGCCCGGGC CCACCGGAGC TTCCTGCAGG AGAGCGAGCG GCTGCTGCTG
    TGTGCAGACA GTATCCAGGC CAAGCTCCAC AGCGAGGAGG AGGCGGTGGA TGTGGCCTCA
    GCCCAGCGGC TGCTGGGGAA GCACCGAGAC CTGCTGGAGG AGATCCACCT GCAGCAGGAG
    AGGCTGCAGC AGCTGGAGAC TCAGGGCCAG CCCATGGCAG CCTCGGGCTC CCCAGACTTC
    CGAGAGGCAG CTAGTACCCT GAGGCTCCTG GGCCACCAAG GCCAGGAGTT GAAGGCCGCC
    TGGGAGCAAA GACAGCAGCA ACTGCAGGAG GGGCTGGAAT TGCAGAAGTT TGGTCGCGAC
    GTGGATGGTT TCACTGCCTC CTGTGCCAAC CATGAGGCCT TCCTACAGCT GGACGTCCTC
    CAGGAGGGTG TGGTGGAGGC CCAGAGTCTG ATGCAGAAGC ACCAGGAGTG TGGGCGGCTG
    CTGGATGCCC TTGGCTCTAG AGCAGAGGAT CTACGGGCAC GTGGCAAGAA GCTGGCTCAG
    AGCCGACACC CCGCTGCACA CAAGGTCAGA GAACAACTGC AGAGCATCCA GGAGCAGTGG
    ACCAGGGTCC AGGAGAGGAG TGAACAGAGA AGGAGGCAGC TGCTGGCCTC CCTCCAGCTC
    CAGGAATGGA AGCAGGACGT GGTGGAGGTG ATGCTGTGGA TGGAGGAGAA GGGGCGGGTG
    TTGGTGGACG AGCCATCTCG AGAGCCCAGC AACATCATGA GGAAACTCAG GCGGCACGAG
    GCAGCCACGT GTGAGCTGGC AGCCACCCGA GGGCACGTGG AGGGCCTGCA GCAGGTCGGG
    AGAGAGCTGC TGAGCAGCAG GCCCCATGCC CAGGAGGACG TGCAGGCCAG GCTTCAGGGC
    CTAAGTAGCA AATGGGAAGA TTTGAGATGC AAGATGGCAG AGCGTGGGAA GCAGCTCCAG
    CAGGCTCGGC AGCAGGACCA GCTCCTGCGG CTGCTACAGG AAGCCAAGGA GAAGATGGAG
    CAGCTAGAGG GGACTGTGCA GAGGGCAGAG GTGGGACAGG ACCTGGGCTC CAGCCAGGGG
    CTGCAGAAAC AGCATTGCCA GCTGGAGGCC GAGAGCCAGG CACTGGCCAG CAAGATGGCT
    GCCCTTGTCC CCGAGGCCCG TCAGGCAATC AGTTCCCAGG CCATCATGGA GGAGACCCAA
    AAATACCTCC AGAGGTTCAA GGCCCTGCAG GGACATCTGG CCAGCCGGCG ACGGCAGCTG
    CAGGCCTCGG TTGAGCTGTA CCACTTCCAC CACCTGAGCA ACGCGGAGCT CATGTGGGTG
    GCAGAGCACA TGCCCACGCC CAGCGCCAGC TCTGCTAAGT GCCCAGATGG TGCCCACCGC
    CTTCTCCACA AGCACAAGGA ACTCCGGGTG CAGGTGAGAG CCCACCAGGG ACAGGTGCGA
    TGGGTGCTGG GCTCCGGACG ATACCTCGCA GCCTCCGGGC ACCCCCAGGC CCAGCACATC
    ATGGAGCGGT GCCAGGAGCT GGAAGGCCGC TGGGCGGATC TGGAGCAGGC GTGTGAGGCA
    CAGGCCCGGC ACCTGCAGCA GGCTGCTGCC CTCCAGGAGT GTTTCCTGGA CGCATCAGAG
    CTGGAGGACT GGGTGGAGGA GAAGTGGCCG CTGGTGAGCA GCCAGGACTA TGGTGGGGAT
    GAGACAGCCA CCATCAGGCT CATTAAGAAG CACCAGGCCC TGCAACAGGA ACTGGCTCGT
    TGTTGGAACT CCGTGGAGGA GCTTGACCGG AGAGCCCAAA CCCTCACTGG CCCCGAGGGC
    CCTGCGCAGC TGGGCGTGGT GCAGAAGAGG CTCCGGGAGC GGCTGCAGGC CCTGCAGGAG
    CTGGCAGCCA CACGGGACCG GGAGCTGGAG GGGACCCTGA AGCTGCATGA GTTCATGAGG
    GAGGCTGAGG ACCTGCAGGG CTGGCTGGCC AGCCAGAAGC AAGTGGCTGG AAGAGGAGAG
    AGCCTTGGGG AAGACTATGA GGACGTCCTG CATCTCTGCA CCAAGTTTTT CAGGTTTCAG
    CGCACAGTAG AGATGGGTGG CCAGCGGGTG GCAAGCTGCC AGCAGCTGGC AGAGAAGCTG
    CTGGAACGAG GGCACAGAGC AGCCCCCAGG GCCCATCAGA TGCAGCAGGA TCTGCAGGCT
    GCCTGGTCAG AGCTATGGGA GCTGACCCAG GCCCGAGGCC GCCTGCTCCG AGACGCTGAG
    GCCACCCTTA AGGTTCACCG AGACATGTTG GAAGCCCTCA CCCAGGTCCA GGAGAAAACC
    ACCAGCCTCC CCTGTGACGT GGCCCAGGAC CTGCGTGGGC TAGAGCCCCA GCTGAGGAGA
    CATGAGGGGC TGGAACGTGA ACTCATGGGC ATTGACCAGC AGCTGCAGGA GCTGCTGGAG
    ATGGGAGGCA CGGTGCAGAA GCTAGGGCCC GGGCCGCAGG CCCATGCAGT ACAGCAGAGG
    CAGCAGGCCC TGGTGCAGGC CTGGGAGACC CTGAAGCTGC GAGCAGAGCA GCGCCGGGCC
    CAGCTCGAGC GGGCATGGCT CCTGGCCCGC TTCCACATGG CGGTGCGGGA CTACACCTCC
    TGGGTGGCCA GCGTGTGGCA GGACCTGCAA GTGGAGGCAG GCTCCCAGGA GCCCGGGAGC
    GGCCCACTGA AGCTCAGCGC CCACCAACAG CTTCGGGCCA TGCTGGAGGC CCGGGAGGAG
    CTGTACCAGC AGGCCGCCCA GCTGGGCCAG CAGGCGCTTC TGGCTGCAGG GACATGCATC
    ACGGAGGTCC GGGACAGGCT GCAAGCCCTG CGGGACAAGC GGGAGCGGGT ATTCCAGGCC
    TGGGAGCAGA AGCAGGAGAG GCTGCAGGCC ATGGGCCAGG AGCAGCTCTT CCTCAGGAAG
    TGTGGCCGCC TGGACGAGAT CCTGAAGGCC CGGGAGGTCC TCCTGAAAAC CAGTGCCTTG
    GGGAGCTCAG TGGAAGAGGT AGAGCAGTTA ATCCGCAAGC ATGAGACCTT CCAGAAGGTG
    CTGGCTGCAC AGGAGGAGAA GGAGGCAGCC CTGTGTGAGC AAGTGAAGAT GCTCGGAGGC
    CCCAGGGCAC AGGATCTGCT TTGCACAGTG CTCGAGCGCC GGGCTCGGGT GAAGGAACTG
    GTGGAGAGTC GGCGACACGC CTTGCACACC TCCCTGATGG TGGCTGCCTT CATCAGGGCC
    GCCACCCAGG CCAAGGACTG GATCCAGGAG CAACTCCAGC AGCTGAAGGA GCCAGTCCCT
    TCTGGGGACC TGAAAGCTAA GTTGAGACAC CTGCAGAAAC ACCAGGCCTT TGAGGCTGAG
    GTCCAGGCCC ATGAGGAAAT CATAACCTCT GTTGTCAAGG AGGGCGAGGC TCTCCTAGCA
    CATAGCCACC CTCTGGTGGG AGAGGTCTCC CAGAGGCTGC GGGAGCTGCA GGAGCACTGG
    GAGAAGTTGA GGCAGGCAGT GGCTCTCCGG GGCGAGGACC TGGAGGACAA GCGGAACATC
    CTGGAGTTCC TGCAGAGAGT GGACACTGCA GAGGCCTGGA TCCAGGAGAT GGAGGTGATG
    GTGAATGTCA GCGACCTGGG CCAGGACCGT GAGCACTGCC AGCAGCTCCA CAGGCAGCTC
    CGCAAGCTCC GAGGAGTCTG GGCCGGGGAC ACGGTGGATG ATGCCCGCAT CAGAAGCATC
    AGTGACTTCT CACCACAGCT CAAGACTCAG GACACAGAGC AACTCAAGAC CATCAGGCAG
    CGGCAGCAGC AGCTCAACAA CAGGCCGGTC CCAGAGCAAG AAGCAGTGGG GCTGGGAGAG
    GGGGCTGAGG GTCCAACCCA GACTCAGCCT TACACCCTCT GCCTCAGGTG GAACAGTTTC
    CATGGCAACC TGCTCAGATA CCAGCAGCAG CTCGAAGTGG CCCTGGAAAT ACACACGTTG
    TCCCAAAAGC TTGATGACAT CACTCAGCAG GTCAGGAAGA AGGCCGCCCT GGTCCAGGCC
    CTGGACTGTG GGAAAGATCT GGACAATGTG CAGAGGCTGA AGCAGAAACA CAAGGAGCTG
    GAGCAGGAAA TGGGCCTCCT CCAGGCCCAG GTGGAGCCTC TAGATCGTGA AGCAGGCCGA
    GTCTGCCAGA GAAGCCCTGA GGTGGCCCAC GGCCTCAGCC ACCAGCAGCA GGAGATGATG
    GACAGCTGGC GGCAGCTCCA GAGCAGGATC CAGAAGTGGA AGGAGTCGCT GGACGCCTTG
    CAGCAAGCTC AGGAGCTGCA GGCAGTGCTT CGGGAACTGC TGGTCTGGGC CCAGAGGCTG
    CGAGCTCAGC TGGACTCCCG GAGAAGCCCC GGCAGCCTCG TGGAGGCCCA GCACATGTTG
    GAGGAACACC AGGAACTCAA AGCTGAGCTG GATTCCCGAA TGGACAGCAT CAGCCTGGCC
    CGGAGCACAG GGCAGCAACT GCTTGCTGCG GGGCACCCAT CCACCCCCAA CATTCGCCAG
    GCCCTGGCTG ATTTTAATCA GGAGCTGAAC AGCCTGGAAG GGGCCTGGCA TGAGCACCAG
    CTTCACCTGC ATCAGGCCCT GGAACTACAG CGGGTTCTGA GCTCTGTGGA GCAGATGGAA
    AGTTGGCTCT CCAGCCTGGA AGCCTGCCCA GCCAGTGAGG GTCTGGGGGA CTGCTTGGCC
    AACGTGGAGA CCCTGCTGTG GAAGCACAGG GTGCGTGAGC AGGACCTGGA GGCTCAGGCG
    GAGAAGATGA GCGCGCTGGA AGCCGCCAGC CGCAGCCTGC TCCAGGGCGG ACACCCCGAG
    GCCCAGGGCA CGCTGAGTCG GTGCCAGGCC ATGCTTCTGA GGAAAGAGGC ACTCCTGGAG
    CGAGCCAGGA CCCGTTGCTG CCAGCTGGAG GAGCTCAGGC AACTGCAGTC TTTCCTGCAG
    GACTCCTGTG AGATGGCCGC CTGGCTGAGG GAGAAGAACC TGGTGGCCCT GGAAGAGGGC
    TGGTGGGACC CAGTGGACCT GCAGGCCCAG TTGCAGCAAC AACAGCATCT CCAGGCGGAG
    CTGGACACCC GTGCACACCA CCAACAGAGG CTGCAGATGG AGGGGCAGAG GCTGCTACAG
    GATGGCCACT TGGCCTCAGA GACCATCCAG GAGCGGCTGC AGGAGCTGAG AAAGCTCTGG
    GAGGAGCTGC AGGCCCAGAG CCAGAGGAGG GCAGCCAAGC TACAGGAGGC CCGTGAGGCC
    CTGCGTCTGC GACGGAGCAT GGAGGAACTG GAGAGCTGGC TGGAGCCTGT GGAGCTCAAG
    CTGAGAGTCC CCATCGGGGG CCAGGACCAG CCTGGGCTGG ATGAGCTCCT GGGGGATCAG
    GGGGAGCTGG AGGCAGCAGT GGACAGGCAG GTCGGGCGGG CACAGGCCCT GCTGGGCCAA
    GCCCAGGCCC AGGCCTGTGT CCAGGAAGGT CACTGCCTGG CCCGAGACGT GGAAGAGCAG
    GCCCAGCGGC TGCTTCAGAG GTTCGAGAGC CTGCGGGCGC CCCTGCAGGA GCGCAGGACA
    GCCCTGGAGG CCCGGAGCCT GCTGCTGCAG TTCTTCAGGG ACGCAGAAGA GGAGATGGCC
    TGGGTGCAGG AGAAGCTGCT CCTGGTGGCC GCCCGGGACT GTGGCCAGAG CCCAAGTGCC
    CTGCGGCGCC TGCAGGAGAA GCACCAGAGC CTGGAGAGTG AGATGAGCAG CCACGAGGCT
    CTAACCCGGG CGGTGGTGGG CACAGGGCGC AAGCTGGTAC AGGCTGGGCA CTTCGCTGCC
    CGCGATGTGG CTGCCCGAGT GCAGCAGCTG GAGGACGCCA TGGGCCGCCT GCGGGCAGAG
    GCTGCACAGA GGCGTCGGCG GCTACAGCAG GCTCAGGAGG CCCAGTACTT CCTGACGGAG
    CTCCTGGAGG CAGAATCCTG GCTGGAAGAA CGGGGCTGTG GCCTGGACAT CGAGGATATG
    GGTCAGAGTG CCGAGGCCAC GCAGGCTTTC CTACGGCAGC TGGAGGCCAC CAGGAGGGAC
    CTGGAGGGAT TTTCCACGCG CATTGAGAGG CTGCAGCAGA CAGCAGCCCT CCTTGAGAGT
    GGGCAGAACC CAGAAAGCCC CAAGGTGCTT GCCCAGATGC ATGCGGTGAG GGACGCCCAC
    TCAGGGCTGC TGCAGAGAGT GGAGAGTAGG GGGCAAGGCC TGAAGGAGCA GCTGCAGCTC
    CACCAGCTGG AGCGGGAAGC CCTGCTCCTG GATGCCTGGC TGGCCAGCAA AGTGGCCACT
    GCCGAGTCCC AGGACTACGG GCAGGACCTA GAGGCTGTCA AGCTGCTGGA AGAGAAGTTC
    GATGCTTTCA GGAAGGATGT CCAGAGCCTG GGGCAGGCTA GGGTGCAGGC CCTGAGGGAG
    CGGGCAGGCT CCCTGGAACG AGCAGCACCC AGGTGCTCTC CTCAGATTCA AGCACAGAGG
    AGTCGCATTG AGGCGTCTTG GGAGAGGCTG GATCAGGCAG TGAAAGCCCG CACACAGAAC
    CTAGCTGCCG CCCGTGAGGT CCGAGGCTTG GAGCAGGCAG CAGCTAAGCT CCAGGCCCGG
    ATGCAAAAGA AGGCCACCCT GGTAGCTAGA GATGCCTTCA ACCTAAGCCT GTCATCAGTG
    CAGACTCTGC AGCAACAGCA CAGATGTCTG GAGAGGGAAC TGGCAGCGAT GGAAAAGGAG
    GTGGCACAGG TGCAGACGGA GGCCTGCCGC CTGGGCCAGC TCTACCCTGC AGCTCAGGAG
    GGCCTGGCCA AGCAGCTGGC CGGGGTGCAG GAGGCCTGGG CCACCCTGAA CGCAAAGGTC
    CATGAGCAAG ATCGGCAGCT GGAGAAGGCT GCCGAGGGCC ATGCCTTCCT CGGACGCTGT
    CGGGAATTGC TAGCATGGGC CACGGAGAAG CAGGCCCTGG TGTCCTCGGA GGAGCTGGCT
    GGGGATATAG CTCGGGCTGA GGGCCTCCTG GCACTGCATG AGAAGCTGGG GAGAGAAATC
    AAAGAGTACT GCCTTCAAGC CCAGAACGTA CAGCAGGAAG GGCAGCGGCT GGTGGACAGT
    GGCCACTGCA TGTCCCTGGA GGTAACTGGG TGTCTGCAGG ATCTGGACAG GCAGCTGCGG
    GCACTCAGAG AGGCCTGGGC CCTGCGCCGG GAACGCTGTG AGGAGAGCTG GCGTCTACAG
    AAGCTGCGGC AAGAGCTGGA CCAGGCCGAG GCCTGGCTGG CCTGCCGGGA GGGCCTCCTG
    CTGGACCCCA ACTGCGGGCA CTCCCTGTCT GATGTGGAGT TGCTGCTCTG CAGACACAAG
    GACTTAGAGA AGCTGCTGGC CTCCCAGGAA GAGAAGTTTG CCCGGCTGCA GCAGGAGGCA
    GAGGTGGAAC AGAAGCAGCA GGTGAAGGGG CTGAAACCCT TGAGAAGCTG GTGGCCCAGA
    GCACAGCTGA CTGAGACAGG GGACCAGCAG CCATCTGTCC CCATTGAGTC TTGCATACGC
    TTCACCGCCG TCTCCCCGGA GTGCACAGCG GGGACCCTGG GGGATGCAAA GGGTGTCCCC
    ACCACGGAGG GAATTTTGGA ACTTAAGCAG CAGCTCCTGC CTGGGAGAAG GCAGAACACG
    GCTTCTACAG CCTCCCTCGA CCTCGTAGGA GTGCAGCGTG AGAAGCCGGG AGATCACCAT
    GATGGAAAAC ACACTTTTTC CTTAAGGCTA ACTGCAGGAG AGACCCTGTC TGCAGCGCCA
    TCTGCAGGAC AGACTGGGAG CTGGCGGGGC ACCCTGAGCA GCTGGACAGC CCCCAGTCTG
    AGCCCAGAGC TCAAAGCCAG ACCCTCAGGC TTCCTGGCTG AGCGCAATGC TGAGAGGGTA
    CCCGGCATGC CAGCTCCACT CAGACTCCAC AGCGGTACCC CGCCCGTCTG GCTGCAGGAG
    ACACAAGTGT GCACCTATAG CTTCCCGCAG CCCGTGCGGC TCCCACAGAC GGCTGGCCTC
    GCAGAGCAAA CTGCAGAAGG CTCCCACCGA CTGTCCTGCT CAGGCCAGAT CCATGGTCTG
    GGCCTCGAGG CAAAAGCAGA GCTGGCACCA AGCCTCAGGG CTACAAGCGA GGACACCCTG
    CTATCTGGCC GTTCAGGACC AACTTCTGGA ACAGCTGGTT CCTCATCCAG GGATGGGCCC
    CACCGAGGTC AGAGCCTGCT CAAGTCACAC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320532.1
    CDS221..10996
    Translation

    Target ORF information:

    RefSeq Version XM_015465046.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GCCCTGGAAG CAGAGCTCCA TCGCCACCAG
    GCCGTGTGTG CTGATCTCGT GCAGAGGGGA TGCAAACTGG GAGCCTGCGG GCCCCCAACG
    GGGCCGGATC CCTGGGAGCG GGCTGAGGTG GTGCAGGGCA TGTGGCAGCG GCTCTGGGCC
    CGGATGGATG AGCAGGGCAT GAGGCTGCAG GCGGCCCTGC TCGTCCAGCA GTACTTCGTG
    GACGTGGCGG AGGCAGACTC GTGGCTTCAG GAGCAGCGGT CTACACTGGA GAGTGCATCC
    TTTGGACAGG ACCAGGTGGC CACCGAGGCC CTACTGCGGC AACACCTGCA TCTGGAACGC
    TCTGTGCGCG CCTTCGGGAC CGAGCTGCGT CGATTAGATG AGCAGGCACA GGTGGCCACA
    GCCCAGGCAG CACTCCTGAG GGAGCCCCCA GAAGGCTGGA GCAGAACTGA TGGAGATGTC
    ACCAGGAGAC CTCAGGATGG GACCCAAATT TTAGCACTGC GGGGCAGCCC TCAGATCAAG
    ACAAGCTTCA CCTACAGAGG CACGCAATTC ACCCAGCTCC CAGGAGAGGC CCAGCAGCCC
    CCACACCAGA TGGACCCCAA CACCCCAGGA GGCTGCCAGG CCACAGTGGC CCTCAGGCAC
    ATCATGGGAC AGGAAGCTCA GGTGGTGTCT GCCCTGAGCT CTCCGAAGAT AGGCCCAAGA
    AACCCAGGGG CCTGGCGTGA GGTTTTCTGC CACTCTGGTC TGTGGCGCAC CCAGAAGATG
    ACCCTCCCAG ATGAGCCTGA CCCTGGCTTT GACCCCAACA CCATCCTCCA GACACAGGAC
    CGCTTGAACC AGGACTATGA GGGCCTGAGG GCCCAGGCAG AGCGCCGCAG GGCCCAGCTG
    GAGGAGGCCG TGGCCCTGTT TGACTTCTAT GGCTCATGTG GGGAGCTGCA GTCCTGGCTG
    ACCAAACAGA CAACGCTGTT GCAAATGCTG CAGCCCGAGG CCAACAACTT GGAAGTTATG
    CAGCTCAAGT ATGAGAACTT CCTCACTGCC CTGGCTGTGG GAAGAGGCCT CTGGACTGAG
    GTTAACAGCT CCGCTGAGCA GCTGAAGCAG AGGTGTCCTG GAGGTTCCCC CCAAATCCAG
    CTACAGCAGG AGGAACTGAG CCAGAGGTGG GAGCAGCTGG AGGCCCTGAA GAAAGAGAAG
    GAAACGCAGC TGGTGTGCAC AACGCACGTG TGCAGTTTTC TGCAGGAGTG TGGATCCACA
    CGGGTCCAGC TGCAAAACCT GGTACTCCAG CTAGAAAGCT TGGAGCTGGG GAACTCAGAG
    GATGGCCACC ATGTTCTGCG AGTGGGCCAG CAGAAGATGC TGGTGCTGGA GAAATACATT
    CGTCACCTGC AAAGGGCAGC TAGCAAGGTT GAGGAGATGG CCCCGGCCGA GAGCCCGTCC
    CTGCAGGCAC ATGTGGAGAC ACTGCAGGGG CTGCTGGAGC GAGTGCGGGA GCAAGTAGCC
    CAGCGGGCCA AGGCCCGGGC CCACCGGAGC TTCCTGCAGG AGAGCGAGCG GCTGCTGCTG
    TGTGCAGACA GTATCCAGGC CAAGCTCCAC AGCGAGGAGG AGGCGGTGGA TGTGGCCTCA
    GCCCAGCGGC TGCTGGGGAA GCACCGAGAC CTGCTGGAGG AGATCCACCT GCAGCAGGAG
    AGGCTGCAGC AGCTGGAGAC TCAGGGCCAG CCCATGGCAG CCTCGGGCTC CCCAGACTTC
    CGAGAGGCAG CTAGTACCCT GAGGCTCCTG GGCCACCAAG GCCAGGAGTT GAAGGCCGCC
    TGGGAGCAAA GACAGCAGCA ACTGCAGGAG GGGCTGGAAT TGCAGAAGTT TGGTCGCGAC
    GTGGATGGTT TCACTGCCTC CTGTGCCAAC CATGAGGCCT TCCTACAGCT GGACGTCCTC
    CAGGAGGGTG TGGTGGAGGC CCAGAGTCTG ATGCAGAAGC ACCAGGAGTG TGGGCGGCTG
    CTGGATGCCC TTGGCTCTAG AGCAGAGGAT CTACGGGCAC GTGGCAAGAA GCTGGCTCAG
    AGCCGACACC CCGCTGCACA CAAGGTCAGA GAACAACTGC AGAGCATCCA GGAGCAGTGG
    ACCAGGGTCC AGGAGAGGAG TGAACAGAGA AGGAGGCAGC TGCTGGCCTC CCTCCAGCTC
    CAGGAATGGA AGCAGGACGT GGTGGAGGTG ATGCTGTGGA TGGAGGAGAA GGGGCGGGTG
    TTGGTGGACG AGCCATCTCG AGAGCCCAGC AACATCATGA GGAAACTCAG GCGGCACGAG
    GCAGCCACGT GTGAGCTGGC AGCCACCCGA GGGCACGTGG AGGGCCTGCA GCAGGTCGGG
    AGAGAGCTGC TGAGCAGCAG GCCCCATGCC CAGGAGGACG TGCAGGCCAG GCTTCAGGGC
    CTAAGTAGCA AATGGGAAGA TTTGAGATGC AAGATGGCAG AGCGTGGGAA GCAGCTCCAG
    CAGGCTCGGC AGCAGGACCA GCTCCTGCGG CTGCTACAGG AAGCCAAGGA GAAGATGGAG
    CAGCTAGAGG GGACTGTGCA GAGGGCAGAG GTGGGACAGG ACCTGGGCTC CAGCCAGGGG
    CTGCAGAAAC AGCATTGCCA GCTGGAGGCC GAGAGCCAGG CACTGGCCAG CAAGATGGCT
    GCCCTTGTCC CCGAGGCCCG TCAGGCAATC AGTTCCCAGG CCATCATGGA GGAGACCCAA
    AAATACCTCC AGAGGTTCAA GGCCCTGCAG GGACATCTGG CCAGCCGGCG ACGGCAGCTG
    CAGGCCTCGG TTGAGCTGTA CCACTTCCAC CACCTGAGCA ACGCGGAGCT CATGTGGGTG
    GCAGAGCACA TGCCCACGCC CAGCGCCAGC TCTGCTAAGT GCCCAGATGG TGCCCACCGC
    CTTCTCCACA AGCACAAGGA ACTCCGGGTG CAGGTGAGAG CCCACCAGGG ACAGGTGCGA
    TGGGTGCTGG GCTCCGGACG ATACCTCGCA GCCTCCGGGC ACCCCCAGGC CCAGCACATC
    ATGGAGCGGT GCCAGGAGCT GGAAGGCCGC TGGGCGGATC TGGAGCAGGC GTGTGAGGCA
    CAGGCCCGGC ACCTGCAGCA GGCTGCTGCC CTCCAGGAGT GTTTCCTGGA CGCATCAGAG
    CTGGAGGACT GGGTGGAGGA GAAGTGGCCG CTGGTGAGCA GCCAGGACTA TGGTGGGGAT
    GAGACAGCCA CCATCAGGCT CATTAAGAAG CACCAGGCCC TGCAACAGGA ACTGGCTCGT
    TGTTGGAACT CCGTGGAGGA GCTTGACCGG AGAGCCCAAA CCCTCACTGG CCCCGAGGGC
    CCTGCGCAGC TGGGCGTGGT GCAGAAGAGG CTCCGGGAGC GGCTGCAGGC CCTGCAGGAG
    CTGGCAGCCA CACGGGACCG GGAGCTGGAG GGGACCCTGA AGCTGCATGA GTTCATGAGG
    GAGGCTGAGG ACCTGCAGGG CTGGCTGGCC AGCCAGAAGC AAGTGGCTGG AAGAGGAGAG
    AGCCTTGGGG AAGACTATGA GGACGTCCTG CATCTCTGCA CCAAGTTTTT CAGGTTTCAG
    CGCACAGTAG AGATGGGTGG CCAGCGGGTG GCAAGCTGCC AGCAGCTGGC AGAGAAGCTG
    CTGGAACGAG GGCACAGAGC AGCCCCCAGG GCCCATCAGA TGCAGCAGGA TCTGCAGGCT
    GCCTGGTCAG AGCTATGGGA GCTGACCCAG GCCCGAGGCC GCCTGCTCCG AGACGCTGAG
    GCCACCCTTA AGGTTCACCG AGACATGTTG GAAGCCCTCA CCCAGGTCCA GGAGAAAACC
    ACCAGCCTCC CCTGTGACGT GGCCCAGGAC CTGCGTGGGC TAGAGCCCCA GCTGAGGAGA
    CATGAGGGGC TGGAACGTGA ACTCATGGGC ATTGACCAGC AGCTGCAGGA GCTGCTGGAG
    ATGGGAGGCA CGGTGCAGAA GCTAGGGCCC GGGCCGCAGG CCCATGCAGT ACAGCAGAGG
    CAGCAGGCCC TGGTGCAGGC CTGGGAGACC CTGAAGCTGC GAGCAGAGCA GCGCCGGGCC
    CAGCTCGAGC GGGCATGGCT CCTGGCCCGC TTCCACATGG CGGTGCGGGA CTACACCTCC
    TGGGTGGCCA GCGTGTGGCA GGACCTGCAA GTGGAGGCAG GCTCCCAGGA GCCCGGGAGC
    GGCCCACTGA AGCTCAGCGC CCACCAACAG CTTCGGGCCA TGCTGGAGGC CCGGGAGGAG
    CTGTACCAGC AGGCCGCCCA GCTGGGCCAG CAGGCGCTTC TGGCTGCAGG GACATGCATC
    ACGGAGGTCC GGGACAGGCT GCAAGCCCTG CGGGACAAGC GGGAGCGGGT ATTCCAGGCC
    TGGGAGCAGA AGCAGGAGAG GCTGCAGGCC ATGGGCCAGG AGCAGCTCTT CCTCAGGAAG
    TGTGGCCGCC TGGACGAGAT CCTGAAGGCC CGGGAGGTCC TCCTGAAAAC CAGTGCCTTG
    GGGAGCTCAG TGGAAGAGGT AGAGCAGTTA ATCCGCAAGC ATGAGACCTT CCAGAAGGTG
    CTGGCTGCAC AGGAGGAGAA GGAGGCAGCC CTGTGTGAGC AAGTGAAGAT GCTCGGAGGC
    CCCAGGGCAC AGGATCTGCT TTGCACAGTG CTCGAGCGCC GGGCTCGGGT GAAGGAACTG
    GTGGAGAGTC GGCGACACGC CTTGCACACC TCCCTGATGG TGGCTGCCTT CATCAGGGCC
    GCCACCCAGG CCAAGGACTG GATCCAGGAG CAACTCCAGC AGCTGAAGGA GCCAGTCCCT
    TCTGGGGACC TGAAAGCTAA GTTGAGACAC CTGCAGAAAC ACCAGGCCTT TGAGGCTGAG
    GTCCAGGCCC ATGAGGAAAT CATAACCTCT GTTGTCAAGG AGGGCGAGGC TCTCCTAGCA
    CATAGCCACC CTCTGGTGGG AGAGGTCTCC CAGAGGCTGC GGGAGCTGCA GGAGCACTGG
    GAGAAGTTGA GGCAGGCAGT GGCTCTCCGG GGCGAGGACC TGGAGGACAA GCGGAACATC
    CTGGAGTTCC TGCAGAGAGT GGACACTGCA GAGGCCTGGA TCCAGGAGAT GGAGGTGATG
    GTGAATGTCA GCGACCTGGG CCAGGACCGT GAGCACTGCC AGCAGCTCCA CAGGCAGCTC
    CGCAAGCTCC GAGGAGTCTG GGCCGGGGAC ACGGTGGATG ATGCCCGCAT CAGAAGCATC
    AGTGACTTCT CACCACAGCT CAAGACTCAG GACACAGAGC AACTCAAGAC CATCAGGCAG
    CGGCAGCAGC AGCTCAACAA CAGGCCGGTC CCAGAGCAAG AAGCAGTGGG GCTGGGAGAG
    GGGGCTGAGG GTCCAACCCA GACTCAGCCT TACACCCTCT GCCTCAGGTG GAACAGTTTC
    CATGGCAACC TGCTCAGATA CCAGCAGCAG CTCGAAGTGG CCCTGGAAAT ACACACGTTG
    TCCCAAAAGC TTGATGACAT CACTCAGCAG GTCAGGAAGA AGGCCGCCCT GGTCCAGGCC
    CTGGACTGTG GGAAAGATCT GGACAATGTG CAGAGGCTGA AGCAGAAACA CAAGGAGCTG
    GAGCAGGAAA TGGGCCTCCT CCAGGCCCAG GTGGAGCCTC TAGATCGTGA AGCAGGCCGA
    GTCTGCCAGA GAAGCCCTGA GGTGGCCCAC GGCCTCAGCC ACCAGCAGCA GGAGATGATG
    GACAGCTGGC GGCAGCTCCA GAGCAGGATC CAGAAGTGGA AGGAGTCGCT GGACGCCTTG
    CAGCAAGCTC AGGAGCTGCA GGCAGTGCTT CGGGAACTGC TGGTCTGGGC CCAGAGGCTG
    CGAGCTCAGC TGGACTCCCG GAGAAGCCCC GGCAGCCTCG TGGAGGCCCA GCACATGTTG
    GAGGAACACC AGGAACTCAA AGCTGAGCTG GATTCCCGAA TGGACAGCAT CAGCCTGGCC
    CGGAGCACAG GGCAGCAACT GCTTGCTGCG GGGCACCCAT CCACCCCCAA CATTCGCCAG
    GCCCTGGCTG ATTTTAATCA GGAGCTGAAC AGCCTGGAAG GGGCCTGGCA TGAGCACCAG
    CTTCACCTGC ATCAGGCCCT GGAACTACAG CGGGTTCTGA GCTCTGTGGA GCAGATGGAA
    AGTTGGCTCT CCAGCCTGGA AGCCTGCCCA GCCAGTGAGG GTCTGGGGGA CTGCTTGGCC
    AACGTGGAGA CCCTGCTGTG GAAGCACAGG GTGCGTGAGC AGGACCTGGA GGCTCAGGCG
    GAGAAGATGA GCGCGCTGGA AGCCGCCAGC CGCAGCCTGC TCCAGGGCGG ACACCCCGAG
    GCCCAGGGCA CGCTGAGTCG GTGCCAGGCC ATGCTTCTGA GGAAAGAGGC ACTCCTGGAG
    CGAGCCAGGA CCCGTTGCTG CCAGCTGGAG GAGCTCAGGC AACTGCAGTC TTTCCTGCAG
    GACTCCTGTG AGATGGCCGC CTGGCTGAGG GAGAAGAACC TGGTGGCCCT GGAAGAGGGC
    TGGTGGGACC CAGTGGACCT GCAGGCCCAG TTGCAGCAAC AACAGCATCT CCAGGCGGAG
    CTGGACACCC GTGCACACCA CCAACAGAGG CTGCAGATGG AGGGGCAGAG GCTGCTACAG
    GATGGCCACT TGGCCTCAGA GACCATCCAG GAGCGGCTGC AGGAGCTGAG AAAGCTCTGG
    GAGGAGCTGC AGGCCCAGAG CCAGAGGAGG GCAGCCAAGC TACAGGAGGC CCGTGAGGCC
    CTGCGTCTGC GACGGAGCAT GGAGGAACTG GAGAGCTGGC TGGAGCCTGT GGAGCTCAAG
    CTGAGAGTCC CCATCGGGGG CCAGGACCAG CCTGGGCTGG ATGAGCTCCT GGGGGATCAG
    GGGGAGCTGG AGGCAGCAGT GGACAGGCAG GTCGGGCGGG CACAGGCCCT GCTGGGCCAA
    GCCCAGGCCC AGGCCTGTGT CCAGGAAGGT CACTGCCTGG CCCGAGACGT GGAAGAGCAG
    GCCCAGCGGC TGCTTCAGAG GTTCGAGAGC CTGCGGGCGC CCCTGCAGGA GCGCAGGACA
    GCCCTGGAGG CCCGGAGCCT GCTGCTGCAG TTCTTCAGGG ACGCAGAAGA GGAGATGGCC
    TGGGTGCAGG AGAAGCTGCT CCTGGTGGCC GCCCGGGACT GTGGCCAGAG CCCAAGTGCC
    CTGCGGCGCC TGCAGGAGAA GCACCAGAGC CTGGAGAGTG AGATGAGCAG CCACGAGGCT
    CTAACCCGGG CGGTGGTGGG CACAGGGCGC AAGCTGGTAC AGGCTGGGCA CTTCGCTGCC
    CGCGATGTGG CTGCCCGAGT GCAGCAGCTG GAGGACGCCA TGGGCCGCCT GCGGGCAGAG
    GCTGCACAGA GGCGTCGGCG GCTACAGCAG GCTCAGGAGG CCCAGTACTT CCTGACGGAG
    CTCCTGGAGG CAGAATCCTG GCTGGAAGAA CGGGGCTGTG GCCTGGACAT CGAGGATATG
    GGTCAGAGTG CCGAGGCCAC GCAGGCTTTC CTACGGCAGC TGGAGGCCAC CAGGAGGGAC
    CTGGAGGGAT TTTCCACGCG CATTGAGAGG CTGCAGCAGA CAGCAGCCCT CCTTGAGAGT
    GGGCAGAACC CAGAAAGCCC CAAGGTGCTT GCCCAGATGC ATGCGGTGAG GGACGCCCAC
    TCAGGGCTGC TGCAGAGAGT GGAGAGTAGG GGGCAAGGCC TGAAGGAGCA GCTGCAGCTC
    CACCAGCTGG AGCGGGAAGC CCTGCTCCTG GATGCCTGGC TGGCCAGCAA AGTGGCCACT
    GCCGAGTCCC AGGACTACGG GCAGGACCTA GAGGCTGTCA AGCTGCTGGA AGAGAAGTTC
    GATGCTTTCA GGAAGGATGT CCAGAGCCTG GGGCAGGCTA GGGTGCAGGC CCTGAGGGAG
    CGGGCAGGCT CCCTGGAACG AGCAGCACCC AGGTGCTCTC CTCAGATTCA AGCACAGAGG
    AGTCGCATTG AGGCGTCTTG GGAGAGGCTG GATCAGGCAG TGAAAGCCCG CACACAGAAC
    CTAGCTGCCG CCCGTGAGGT CCGAGGCTTG GAGCAGGCAG CAGCTAAGCT CCAGGCCCGG
    ATGCAAAAGA AGGCCACCCT GGTAGCTAGA GATGCCTTCA ACCTAAGCCT GTCATCAGTG
    CAGACTCTGC AGCAACAGCA CAGATGTCTG GAGAGGGAAC TGGCAGCGAT GGAAAAGGAG
    GTGGCACAGG TGCAGACGGA GGCCTGCCGC CTGGGCCAGC TCTACCCTGC AGCTCAGGAG
    GGCCTGGCCA AGCAGCTGGC CGGGGTGCAG GAGGCCTGGG CCACCCTGAA CGCAAAGGTC
    CATGAGCAAG ATCGGCAGCT GGAGAAGGCT GCCGAGGGCC ATGCCTTCCT CGGACGCTGT
    CGGGAATTGC TAGCATGGGC CACGGAGAAG CAGGCCCTGG TGTCCTCGGA GGAGCTGGCT
    GGGGATATAG CTCGGGCTGA GGGCCTCCTG GCACTGCATG AGAAGCTGGG GAGAGAAATC
    AAAGAGTACT GCCTTCAAGC CCAGAACGTA CAGCAGGAAG GGCAGCGGCT GGTGGACAGT
    GGCCACTGCA TGTCCCTGGA GGTAACTGGG TGTCTGCAGG ATCTGGACAG GCAGCTGCGG
    GCACTCAGAG AGGCCTGGGC CCTGCGCCGG GAACGCTGTG AGGAGAGCTG GCGTCTACAG
    AAGCTGCGGC AAGAGCTGGA CCAGGCCGAG GCCTGGCTGG CCTGCCGGGA GGGCCTCCTG
    CTGGACCCCA ACTGCGGGCA CTCCCTGTCT GATGTGGAGT TGCTGCTCTG CAGACACAAG
    GACTTAGAGA AGCTGCTGGC CTCCCAGGAA GAGAAGTTTG CCCGGCTGCA GCAGGAGGCA
    GAGGTGGAAC AGAAGCAGCA GGTGAAGGGG CTGAAACCCT TGAGAAGCTG GTGGCCCAGA
    GCACAGCTGA CTGAGACAGG GGACCAGCAG CCATCTGTCC CCATTGAGTC TTGCATACGC
    TTCACCGCCG TCTCCCCGGA GTGCACAGCG GGGACCCTGG GGGATGCAAA GGGTGTCCCC
    ACCACGGAGG GAATTTTGGA ACTTAAGCAG CAGCTCCTGC CTGGGAGAAG GCAGAACACG
    GCTTCTACAG CCTCCCTCGA CCTCGTAGGA GTGCAGCGTG AGAAGCCGGG AGATCACCAT
    GATGGAAAAC ACACTTTTTC CTTAAGGCTA ACTGCAGGAG AGACCCTGTC TGCAGCGCCA
    TCTGCAGGAC AGACTGGGAG CTGGCGGGGC ACCCTGAGCA GCTGGACAGC CCCCAGTCTG
    AGCCCAGAGC TCAAAGCCAG ACCCTCAGGC TTCCTGGCTG AGCGCAATGC TGAGAGGGTA
    CCCGGCATGC CAGCTCCACT CAGACTCCAC AGCGGTACCC CGCCCGTCTG GCTGCAGGAG
    ACACAAGTGT GCACCTATAG CTTCCCGCAG CCCGTGCGGC TCCCACAGAC GGCTGGCCTC
    GCAGAGCAAA CTGCAGAAGG CTCCCACCGA CTGTCCTGCT CAGGCCAGAT CCATGGTCTG
    GGCCTCGAGG CAAAAGCAGA GCTGGCACCA AGCCTCAGGG CTACAAGCGA GGACACCCTG
    CTATCTGGCC GTTCAGGACC AACTTCTGGA ACAGCTGGTT CCTCATCCAG GGATGGGCCC
    CACCGAGGTC AGAGCCTGCT CAAGTCACAC CCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91677
    Clone ID Related Accession (Same CDS sequence) XM_015465047.1 , XM_015473135.1
    Accession Version XM_015465047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG CTGCAAGGTC CTGTCACCCC ACATCTGGGT
    GGGCTGGTGC ACAGTGTGGA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320533.1
    CDS241..10347
    Translation

    Target ORF information:

    RefSeq Version XM_015465047.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG CTGCAAGGTC CTGTCACCCC ACATCTGGGT
    GGGCTGGTGC ACAGTGTGGA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91677
    Clone ID Related Accession (Same CDS sequence) XM_015465047.1 , XM_015473135.1
    Accession Version XM_015473135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG CTGCAAGGTC CTGTCACCCC ACATCTGGGT
    GGGCTGGTGC ACAGTGTGGA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328621.1
    CDS241..10347
    Translation

    Target ORF information:

    RefSeq Version XM_015473135.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAACCTA GCTGCCGCCC GTGAGGTCCG AGGCTTGGAG
    CAGGCAGCAG CTAAGCTCCA GGCCCGGATG CAAAAGAAGG CCACCCTGGT AGCTAGAGAT
    GCCTTCAACC TAAGCCTGTC ATCAGTGCAG ACTCTGCAGC AACAGCACAG ATGTCTGGAG
    AGGGAACTGG CAGCGATGGA AAAGGAGGTG GCACAGGTGC AGACGGAGGC CTGCCGCCTG
    GGCCAGCTCT ACCCTGCAGC TCAGGAGGGC CTGGCCAAGC AGCTGGCCGG GGTGCAGGAG
    GCCTGGGCCA CCCTGAACGC AAAGGTCCAT GAGCAAGATC GGCAGCTGGA GAAGGCTGCC
    GAGGGCCATG CCTTCCTCGG ACGCTGTCGG GAATTGCTAG CATGGGCCAC GGAGAAGCAG
    GCCCTGGTGT CCTCGGAGGA GCTGGCTGGG GATATAGCTC GGGCTGAGGG CCTCCTGGCA
    CTGCATGAGA AGCTGGGGAG AGAAATCAAA GAGTACTGCC TTCAAGCCCA GAACGTACAG
    CAGGAAGGGC AGCGGCTGGT GGACAGTGGC CACTGCATGT CCCTGGAGGT AACTGGGTGT
    CTGCAGGATC TGGACAGGCA GCTGCGGGCA CTCAGAGAGG CCTGGGCCCT GCGCCGGGAA
    CGCTGTGAGG AGAGCTGGCG TCTACAGAAG CTGCGGCAAG AGCTGGACCA GGCCGAGGCC
    TGGCTGGCCT GCCGGGAGGG CCTCCTGCTG GACCCCAACT GCGGGCACTC CCTGTCTGAT
    GTGGAGTTGC TGCTCTGCAG ACACAAGGAC TTAGAGAAGC TGCTGGCCTC CCAGGAAGAG
    AAGTTTGCCC GGCTGCAGCA GGAGGCAGAG CTGCAAGGTC CTGTCACCCC ACATCTGGGT
    GGGCTGGTGC ACAGTGTGGA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91678
    Clone ID Related Accession (Same CDS sequence) XM_015473136.1 , XM_015465048.1
    Accession Version XM_015473136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9001
    9061
    9121
    9181
    9241
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAGCATG GGCCACGGAG AAGCAGGCCC TGGTGTCCTC
    GGAGGAGCTG GCTGGGGATA TAGCTCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328622.1
    CDS247..9519
    Translation

    Target ORF information:

    RefSeq Version XM_015473136.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAGCATG GGCCACGGAG AAGCAGGCCC TGGTGTCCTC
    GGAGGAGCTG GCTGGGGATA TAGCTCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91678
    Clone ID Related Accession (Same CDS sequence) XM_015473136.1 , XM_015465048.1
    Accession Version XM_015465048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAGCATG GGCCACGGAG AAGCAGGCCC TGGTGTCCTC
    GGAGGAGCTG GCTGGGGATA TAGCTCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320534.1
    CDS247..9519
    Translation

    Target ORF information:

    RefSeq Version XM_015465048.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTTTCTGG GGGGCTTTCT TACCCTCTGG GCACGATGGC AGAGGCTGAG GCCTGGGAGG 
    GTTGTCCTCA GGATCCTGCT TCAGCTGCAG GAGTCAGAGG CGCTGCAGAC CCAGTACAAG
    GAGCTGGTGG CCGACCTGCT GTGCTGGATT GCAGAGAAGC AGGTGCAGCT GGAGGCGCGG
    GATTTCCCAG ACAACCTGCC CGCCATGCGC CAGCTACTGG TGGCCTTTGC CTCCTTCCGC
    AGCCAGGAGA AGCCACCGAG ACTGCAGCAG CGTGGGGCCA CGGAGGCCCT GCTCTTTCGG
    CTGCAGACAG TCCTCCAAGC CCAGAACCGC AGGCCCTTCC TGCCACCTGA GGGCCTGGGC
    CCTGCAGAGC TGTCCCGGTG CTGGACAAGC CTGGAGCGGG CGGAGGCCTC ACGGAGCCGG
    GCCCTGCAGC AGAGGCTCCT ACAGCTAGAG CGGTTGGAAA CCCTGGCCCG GCGTTTCCAG
    CGTAAGGCAG CCCTCCGGGA GAGCTTCCTG ACAGACACGG AGCAGGTGCT AGACCGGGCC
    GCAGCCCCGC TGGCCAGCCG GGCCATGCTG GAAGCGGCCG CCCAGAGGCT AGGCATGCTG
    GAAGCCAGTA TCCTGCCCCA GGAGGGGCGC TTCCAGGCCC TGGCCGAGAT TGCGGACATC
    CTCCATCAGG AGCGCTACCA CGGTGGGACA GAGGTGGCCA GCAGGCAGCT GGAGATCACC
    CAGCGCTGGG AGCGGCTCCT CCAGCGGCTC CAAGGGCAGA GGAAGCAGAT AGCGGGCATG
    CAGGCTGTGC TGAGCCTGCT GCAGGAGGTG GAGACTGCCT CCGACCAGCT CAACGAGCTG
    CAGGTGCTAG TCAGCTCCAT GGCCTGTGGG CAGAAGCTGA CAGAGGTAGA GAGGCTGCTG
    CAGAGGCATG AGCTGCTGGA GGCCCAGATC TCAGCCCTTG GAGCCCACGT GAGCCATCTG
    GCCCACCAGA CGATGAAGCT GGACTCTTCC ATGGGCACTG CTGTGGAGGT GCTGCAGGCC
    AAGGCCAGGG CGCTGGCCCA GCTCTACCAG AGCCTCATGT CTCTTGCTAG GTCCAGGAGG
    GCCTCACTGG AGCAGACGCT GCAGCAGGCA GAGCTCCTGC ACAGCTGTGA GGAGGAGGAA
    GCCTGGTTGA GGGAGCATGG ACAGCTGGTG GAGGATGCGT CCCCGGGCCC GGATCTCAGC
    CAGATCGCAG CGGCCCTGCA GAAACACAAG GTGCTCTCTA CCTTGCATCC TGGCTCTTGC
    CCTCTCCCTG CCATCCATCC CTTCCCTCAC CCAGCTCCCT CCTCTGTGCT CCTCCAGGCC
    CTGGAAGCAG AGCTCCATCG CCACCAGGCC GTGTGTGCTG ATCTCGTGCA GAGGGGATGC
    AAACTGGGAG CCTGCGGGCC CCCAACGGGG CCGGATCCCT GGGAGCGGGC TGAGGTGGTG
    CAGGGCATGT GGCAGCGGCT CTGGGCCCGG ATGGATGAGC AGGGCATGAG GCTGCAGGCG
    GCCCTGCTCG TCCAGCAGTA CTTCGTGGAC GTGGCGGAGG CAGACTCGTG GCTTCAGGAG
    CAGCGGTCTA CACTGGAGAG TGCATCCTTT GGACAGGACC AGGTGGCCAC CGAGGCCCTA
    CTGCGGCAAC ACCTGCATCT GGAACGCTCT GTGCGCGCCT TCGGGACCGA GCTGCGTCGA
    TTAGATGAGC AGGCACAGGT GGCCACAGCC CAGGCAGCAC TCCTGAGGGA GCCCCCAGAA
    GGCTGGAGCA GAACTGATGG AGATGTCACC AGGAGACCTC AGGATGGGAC CCAAATTTTA
    GCACTGCGGG GCAGCCCTCA GATCAAGACA AGCTTCACCT ACAGAGGCAC GCAATTCACC
    CAGCTCCCAG GAGAGGCCCA GCAGCCCCCA CACCAGATGG ACCCCAACAC CCCAGGAGGC
    TGCCAGGCCA CAGTGGCCCT CAGGCACATC ATGGGACAGG AAGCTCAGGT GGTGTCTGCC
    CTGAGCTCTC CGAAGATAGG CCCAAGAAAC CCAGGGGCCT GGCGTGAGGT TTTCTGCCAC
    TCTGGTCTGT GGCGCACCCA GAAGATGACC CTCCCAGATG AGCCTGACCC TGGCTTTGAC
    CCCAACACCA TCCTCCAGAC ACAGGACCGC TTGAACCAGG ACTATGAGGG CCTGAGGGCC
    CAGGCAGAGC GCCGCAGGGC CCAGCTGGAG GAGGCCGTGG CCCTGTTTGA CTTCTATGGC
    TCATGTGGGG AGCTGCAGTC CTGGCTGACC AAACAGACAA CGCTGTTGCA AATGCTGCAG
    CCCGAGGCCA ACAACTTGGA AGTTATGCAG CTCAAGTATG AGAACTTCCT CACTGCCCTG
    GCTGTGGGAA GAGGCCTCTG GACTGAGGTT AACAGCTCCG CTGAGCAGCT GAAGCAGAGG
    TGTCCTGGAG GTTCCCCCCA AATCCAGCTA CAGCAGGAGG AACTGAGCCA GAGGTGGGAG
    CAGCTGGAGG CCCTGAAGAA AGAGAAGGAA ACGCAGCTGG TGTGCACAAC GCACGTGTGC
    AGTTTTCTGC AGGAGTGTGG ATCCACACGG GTCCAGCTGC AAAACCTGGT ACTCCAGCTA
    GAAAGCTTGG AGCTGGGGAA CTCAGAGGAT GGCCACCATG TTCTGCGAGT GGGCCAGCAG
    AAGATGCTGG TGCTGGAGAA ATACATTCGT CACCTGCAAA GGGCAGCTAG CAAGGTTGAG
    GAGATGGCCC CGGCCGAGAG CCCGTCCCTG CAGGCACATG TGGAGACACT GCAGGGGCTG
    CTGGAGCGAG TGCGGGAGCA AGTAGCCCAG CGGGCCAAGG CCCGGGCCCA CCGGAGCTTC
    CTGCAGGAGA GCGAGCGGCT GCTGCTGTGT GCAGACAGTA TCCAGGCCAA GCTCCACAGC
    GAGGAGGAGG CGGTGGATGT GGCCTCAGCC CAGCGGCTGC TGGGGAAGCA CCGAGACCTG
    CTGGAGGAGA TCCACCTGCA GCAGGAGAGG CTGCAGCAGC TGGAGACTCA GGGCCAGCCC
    ATGGCAGCCT CGGGCTCCCC AGACTTCCGA GAGGCAGCTA GTACCCTGAG GCTCCTGGGC
    CACCAAGGCC AGGAGTTGAA GGCCGCCTGG GAGCAAAGAC AGCAGCAACT GCAGGAGGGG
    CTGGAATTGC AGAAGTTTGG TCGCGACGTG GATGGTTTCA CTGCCTCCTG TGCCAACCAT
    GAGGCCTTCC TACAGCTGGA CGTCCTCCAG GAGGGTGTGG TGGAGGCCCA GAGTCTGATG
    CAGAAGCACC AGGAGTGTGG GCGGCTGCTG GATGCCCTTG GCTCTAGAGC AGAGGATCTA
    CGGGCACGTG GCAAGAAGCT GGCTCAGAGC CGACACCCCG CTGCACACAA GGTCAGAGAA
    CAACTGCAGA GCATCCAGGA GCAGTGGACC AGGGTCCAGG AGAGGAGTGA ACAGAGAAGG
    AGGCAGCTGC TGGCCTCCCT CCAGCTCCAG GAATGGAAGC AGGACGTGGT GGAGGTGATG
    CTGTGGATGG AGGAGAAGGG GCGGGTGTTG GTGGACGAGC CATCTCGAGA GCCCAGCAAC
    ATCATGAGGA AACTCAGGCG GCACGAGGCA GCCACGTGTG AGCTGGCAGC CACCCGAGGG
    CACGTGGAGG GCCTGCAGCA GGTCGGGAGA GAGCTGCTGA GCAGCAGGCC CCATGCCCAG
    GAGGACGTGC AGGCCAGGCT TCAGGGCCTA AGTAGCAAAT GGGAAGATTT GAGATGCAAG
    ATGGCAGAGC GTGGGAAGCA GCTCCAGCAG GCTCGGCAGC AGGACCAGCT CCTGCGGCTG
    CTACAGGAAG CCAAGGAGAA GATGGAGCAG CTAGAGGGGA CTGTGCAGAG GGCAGAGGTG
    GGACAGGACC TGGGCTCCAG CCAGGGGCTG CAGAAACAGC ATTGCCAGCT GGAGGCCGAG
    AGCCAGGCAC TGGCCAGCAA GATGGCTGCC CTTGTCCCCG AGGCCCGTCA GGCAATCAGT
    TCCCAGGCCA TCATGGAGGA GACCCAAAAA TACCTCCAGA GGTTCAAGGC CCTGCAGGGA
    CATCTGGCCA GCCGGCGACG GCAGCTGCAG GCCTCGGTTG AGCTGTACCA CTTCCACCAC
    CTGAGCAACG CGGAGCTCAT GTGGGTGGCA GAGCACATGC CCACGCCCAG CGCCAGCTCT
    GCTAAGTGCC CAGATGGTGC CCACCGCCTT CTCCACAAGC ACAAGGAACT CCGGGTGCAG
    GTGAGAGCCC ACCAGGGACA GGTGCGATGG GTGCTGGGCT CCGGACGATA CCTCGCAGCC
    TCCGGGCACC CCCAGGCCCA GCACATCATG GAGCGGTGCC AGGAGCTGGA AGGCCGCTGG
    GCGGATCTGG AGCAGGCGTG TGAGGCACAG GCCCGGCACC TGCAGCAGGC TGCTGCCCTC
    CAGGAGTGTT TCCTGGACGC ATCAGAGCTG GAGGACTGGG TGGAGGAGAA GTGGCCGCTG
    GTGAGCAGCC AGGACTATGG TGGGGATGAG ACAGCCACCA TCAGGCTCAT TAAGAAGCAC
    CAGGCCCTGC AACAGGAACT GGCTCGTTGT TGGAACTCCG TGGAGGAGCT TGACCGGAGA
    GCCCAAACCC TCACTGGCCC CGAGGGCCCT GCGCAGCTGG GCGTGGTGCA GAAGAGGCTC
    CGGGAGCGGC TGCAGGCCCT GCAGGAGCTG GCAGCCACAC GGGACCGGGA GCTGGAGGGG
    ACCCTGAAGC TGCATGAGTT CATGAGGGAG GCTGAGGACC TGCAGGGCTG GCTGGCCAGC
    CAGAAGCAAG TGGCTGGAAG AGGAGAGAGC CTTGGGGAAG ACTATGAGGA CGTCCTGCAT
    CTCTGCACCA AGTTTTTCAG GTTTCAGCGC ACAGTAGAGA TGGGTGGCCA GCGGGTGGCA
    AGCTGCCAGC AGCTGGCAGA GAAGCTGCTG GAACGAGGGC ACAGAGCAGC CCCCAGGGCC
    CATCAGATGC AGCAGGATCT GCAGGCTGCC TGGTCAGAGC TATGGGAGCT GACCCAGGCC
    CGAGGCCGCC TGCTCCGAGA CGCTGAGGCC ACCCTTAAGG TTCACCGAGA CATGTTGGAA
    GCCCTCACCC AGGTCCAGGA GAAAACCACC AGCCTCCCCT GTGACGTGGC CCAGGACCTG
    CGTGGGCTAG AGCCCCAGCT GAGGAGACAT GAGGGGCTGG AACGTGAACT CATGGGCATT
    GACCAGCAGC TGCAGGAGCT GCTGGAGATG GGAGGCACGG TGCAGAAGCT AGGGCCCGGG
    CCGCAGGCCC ATGCAGTACA GCAGAGGCAG CAGGCCCTGG TGCAGGCCTG GGAGACCCTG
    AAGCTGCGAG CAGAGCAGCG CCGGGCCCAG CTCGAGCGGG CATGGCTCCT GGCCCGCTTC
    CACATGGCGG TGCGGGACTA CACCTCCTGG GTGGCCAGCG TGTGGCAGGA CCTGCAAGTG
    GAGGCAGGCT CCCAGGAGCC CGGGAGCGGC CCACTGAAGC TCAGCGCCCA CCAACAGCTT
    CGGGCCATGC TGGAGGCCCG GGAGGAGCTG TACCAGCAGG CCGCCCAGCT GGGCCAGCAG
    GCGCTTCTGG CTGCAGGGAC ATGCATCACG GAGGTCCGGG ACAGGCTGCA AGCCCTGCGG
    GACAAGCGGG AGCGGGTATT CCAGGCCTGG GAGCAGAAGC AGGAGAGGCT GCAGGCCATG
    GGCCAGGAGC AGCTCTTCCT CAGGAAGTGT GGCCGCCTGG ACGAGATCCT GAAGGCCCGG
    GAGGTCCTCC TGAAAACCAG TGCCTTGGGG AGCTCAGTGG AAGAGGTAGA GCAGTTAATC
    CGCAAGCATG AGACCTTCCA GAAGGTGCTG GCTGCACAGG AGGAGAAGGA GGCAGCCCTG
    TGTGAGCAAG TGAAGATGCT CGGAGGCCCC AGGGCACAGG ATCTGCTTTG CACAGTGCTC
    GAGCGCCGGG CTCGGGTGAA GGAACTGGTG GAGAGTCGGC GACACGCCTT GCACACCTCC
    CTGATGGTGG CTGCCTTCAT CAGGGCCGCC ACCCAGGCCA AGGACTGGAT CCAGGAGCAA
    CTCCAGCAGC TGAAGGAGCC AGTCCCTTCT GGGGACCTGA AAGCTAAGTT GAGACACCTG
    CAGAAACACC AGGCCTTTGA GGCTGAGGTC CAGGCCCATG AGGAAATCAT AACCTCTGTT
    GTCAAGGAGG GCGAGGCTCT CCTAGCACAT AGCCACCCTC TGGTGGGAGA GGTCTCCCAG
    AGGCTGCGGG AGCTGCAGGA GCACTGGGAG AAGTTGAGGC AGGCAGTGGC TCTCCGGGGC
    GAGGACCTGG AGGACAAGCG GAACATCCTG GAGTTCCTGC AGAGAGTGGA CACTGCAGAG
    GCCTGGATCC AGGAGATGGA GGTGATGGTG AATGTCAGCG ACCTGGGCCA GGACCGTGAG
    CACTGCCAGC AGCTCCACAG GCAGCTCCGC AAGCTCCGAG GAGTCTGGGC CGGGGACACG
    GTGGATGATG CCCGCATCAG AAGCATCAGT GACTTCTCAC CACAGCTCAA GACTCAGGAC
    ACAGAGCAAC TCAAGACCAT CAGGCAGCGG CAGCAGCAGC TCAACAACAG GCCGGTCCCA
    GAGCAAGAAG CAGTGGGGCT GGGAGAGGGG GCTGAGGGTC CAACCCAGAC TCAGCCTTAC
    ACCCTCTGCC TCAGGTGGAA CAGTTTCCAT GGCAACCTGC TCAGATACCA GCAGCAGCTC
    GAAGTGGCCC TGGAAATACA CACGTTGTCC CAAAAGCTTG ATGACATCAC TCAGCAGGTC
    AGGAAGAAGG CCGCCCTGGT CCAGGCCCTG GACTGTGGGA AAGATCTGGA CAATGTGCAG
    AGGCTGAAGC AGAAACACAA GGAGCTGGAG CAGGAAATGG GCCTCCTCCA GGCCCAGGTG
    GAGCCTCTAG ATCGTGAAGC AGGCCGAGTC TGCCAGAGAA GCCCTGAGGT GGCCCACGGC
    CTCAGCCACC AGCAGCAGGA GATGATGGAC AGCTGGCGGC AGCTCCAGAG CAGGATCCAG
    AAGTGGAAGG AGTCGCTGGA CGCCTTGCAG CAAGCTCAGG AGCTGCAGGC AGTGCTTCGG
    GAACTGCTGG TCTGGGCCCA GAGGCTGCGA GCTCAGCTGG ACTCCCGGAG AAGCCCCGGC
    AGCCTCGTGG AGGCCCAGCA CATGTTGGAG GAACACCAGG AACTCAAAGC TGAGCTGGAT
    TCCCGAATGG ACAGCATCAG CCTGGCCCGG AGCACAGGGC AGCAACTGCT TGCTGCGGGG
    CACCCATCCA CCCCCAACAT TCGCCAGGCC CTGGCTGATT TTAATCAGGA GCTGAACAGC
    CTGGAAGGGG CCTGGCATGA GCACCAGCTT CACCTGCATC AGGCCCTGGA ACTACAGCGG
    GTTCTGAGCT CTGTGGAGCA GATGGAAAGT TGGCTCTCCA GCCTGGAAGC CTGCCCAGCC
    AGTGAGGGTC TGGGGGACTG CTTGGCCAAC GTGGAGACCC TGCTGTGGAA GCACAGGGTG
    CGTGAGCAGG ACCTGGAGGC TCAGGCGGAG AAGATGAGCG CGCTGGAAGC CGCCAGCCGC
    AGCCTGCTCC AGGGCGGACA CCCCGAGGCC CAGGGCACGC TGAGTCGGTG CCAGGCCATG
    CTTCTGAGGA AAGAGGCACT CCTGGAGCGA GCCAGGACCC GTTGCTGCCA GCTGGAGGAG
    CTCAGGCAAC TGCAGTCTTT CCTGCAGGAC TCCTGTGAGA TGGCCGCCTG GCTGAGGGAG
    AAGAACCTGG TGGCCCTGGA AGAGGGCTGG TGGGACCCAG TGGACCTGCA GGCCCAGTTG
    CAGCAACAAC AGCATCTCCA GGCGGAGCTG GACACCCGTG CACACCACCA ACAGAGGCTG
    CAGATGGAGG GGCAGAGGCT GCTACAGGAT GGCCACTTGG CCTCAGAGAC CATCCAGGAG
    CGGCTGCAGG AGCTGAGAAA GCTCTGGGAG GAGCTGCAGG CCCAGAGCCA GAGGAGGGCA
    GCCAAGCTAC AGGAGGCCCG TGAGGCCCTG CGTCTGCGAC GGAGCATGGA GGAACTGGAG
    AGCTGGCTGG AGCCTGTGGA GCTCAAGCTG AGAGTCCCCA TCGGGGGCCA GGACCAGCCT
    GGGCTGGATG AGCTCCTGGG GGATCAGGGG GAGCTGGAGG CAGCAGTGGA CAGGCAGGTC
    GGGCGGGCAC AGGCCCTGCT GGGCCAAGCC CAGGCCCAGG CCTGTGTCCA GGAAGGTCAC
    TGCCTGGCCC GAGACGTGGA AGAGCAGGCC CAGCGGCTGC TTCAGAGGTT CGAGAGCCTG
    CGGGCGCCCC TGCAGGAGCG CAGGACAGCC CTGGAGGCCC GGAGCCTGCT GCTGCAGTTC
    TTCAGGGACG CAGAAGAGGA GATGGCCTGG GTGCAGGAGA AGCTGCTCCT GGTGGCCGCC
    CGGGACTGTG GCCAGAGCCC AAGTGCCCTG CGGCGCCTGC AGGAGAAGCA CCAGAGCCTG
    GAGAGTGAGA TGAGCAGCCA CGAGGCTCTA ACCCGGGCGG TGGTGGGCAC AGGGCGCAAG
    CTGGTACAGG CTGGGCACTT CGCTGCCCGC GATGTGGCTG CCCGAGTGCA GCAGCTGGAG
    GACGCCATGG GCCGCCTGCG GGCAGAGGCT GCACAGAGGC GTCGGCGGCT ACAGCAGGCT
    CAGGAGGCCC AGTACTTCCT GACGGAGCTC CTGGAGGCAG AATCCTGGCT GGAAGAACGG
    GGCTGTGGCC TGGACATCGA GGATATGGGT CAGAGTGCCG AGGCCACGCA GGCTTTCCTA
    CGGCAGCTGG AGGCCACCAG GAGGGACCTG GAGGGATTTT CCACGCGCAT TGAGAGGCTG
    CAGCAGACAG CAGCCCTCCT TGAGAGTGGG CAGAACCCAG AAAGCCCCAA GGTGCTTGCC
    CAGATGCATG CGGTGAGGGA CGCCCACTCA GGGCTGCTGC AGAGAGTGGA GAGTAGGGGG
    CAAGGCCTGA AGGAGCAGCT GCAGCTCCAC CAGCTGGAGC GGGAAGCCCT GCTCCTGGAT
    GCCTGGCTGG CCAGCAAAGT GGCCACTGCC GAGTCCCAGG ACTACGGGCA GGACCTAGAG
    GCTGTCAAGC TGCTGGAAGA GAAGTTCGAT GCTTTCAGGA AGGATGTCCA GAGCCTGGGG
    CAGGCTAGGG TGCAGGCCCT GAGGGAGCGG GCAGGCTCCC TGGAACGAGC AGCACCCAGG
    TGCTCTCCTC AGATTCAAGC ACAGAGGAGT CGCATTGAGG CGTCTTGGGA GAGGCTGGAT
    CAGGCAGTGA AAGCCCGCAC ACAGAGCATG GGCCACGGAG AAGCAGGCCC TGGTGTCCTC
    GGAGGAGCTG GCTGGGGATA TAGCTCGGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91679
    Clone ID Related Accession (Same CDS sequence) XM_015465049.1 , XM_015473137.2 , XM_015473137.1
    Accession Version XM_015465049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645871.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    10921
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    11101
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    11221
    11281
    11341
    11401
    11461
    ATGGACTCTG CGTACGAGAT GGGCCACATC CGCAAGCTGC AGGCGCGGCA CATGCAGATG 
    CAGGAGAAGA CCTTCACCAA GTGGATCAAC AATATCTTCC AGCATGGCCG GGTGGGCATC
    GAGATCCAGA ACCTATGCAC AGAGCTGGCC GACGGCACCC ACCTGCTGCG GCTCCTGGAG
    CTCATCTCAG GGGAGGCCCT GCCACCCCCC AACAGGGGCC GCATGCGCGT CCATTTTCTG
    GAGAACAATA GCCGAGCTCT GGCTTTCCTC AGGGCCAAGG TGCCAATACC CCTCATTGGG
    CCAGAGAACA TCGTGGATGG AGACCAGACG CTCATCCTGG GACTCATCTG GGTCATCATT
    CTGCGTTTCC AGATTTCCGA CATCTGCCTG GACAAGGAGG AGTTTGGGGT CAGCGCAGCC
    CTGCTGTCTG CCAGGGAAGC TCTGCTGGTC TGGTGCCAGC GGAAGACTGC CGGCTATGCC
    AACATCAACA TCACGGACTT CTCCCGCAGC TGGAGTGACG GGCTCGGCTT CAGTGCCCTC
    ATCCATGCCC ACAGGCCAGA CCTGCTTGAC TACAGCTCCC TGCGTCCCGA CCGCCCGCTG
    CACAACCTCC GCTGTGCTTT CCACGTAGCT GAGCAGGAGC TGGGCATTGC TCAGCTGCTG
    GATCCAGAGG ATGTGGCAGC CCTACAGCCT GACGAGCGCT CCATAATGAC CTACGTCTCC
    CTCTACTACC ACCACTTCTC CCGCCTGCAC CAGGAGCAGA CCGTCCAGAG GAGACTCACA
    AAGATCCTGC TTCAGCTGCA GGAGTCAGAG GCGCTGCAGA CCCAGTACAA GGAGCTGGTG
    GCCGACCTGC TGTGCTGGAT TGCAGAGAAG CAGGTGCAGC TGGAGGCGCG GGATTTCCCA
    GACAACCTGC CCGCCATGCG CCAGCTACTG GTGGCCTTTG CCTCCTTCCG CAGCCAGGAG
    AAGCCACCGA GACTGCAGCA GCGTGGGGCC ACGGAGGCCC TGCTCTTTCG GCTGCAGACA
    GTCCTCCAAG CCCAGAACCG CAGGCCCTTC CTGCCACCTG AGGGCCTGGG CCCTGCAGAG
    CTGTCCCGGT GCTGGACAAG CCTGGAGCGG GCGGAGGCCT CACGGAGCCG GGCCCTGCAG
    CAGAGGCTCC TACAGCTAGA GCGGTTGGAA ACCCTGGCCC GGCGTTTCCA GCGTAAGGCA
    GCCCTCCGGG AGAGCTTCCT GACAGACACG GAGCAGGTGC TAGACCGGGC CGCAGCCCCG
    CTGGCCAGCC GGGCCATGCT GGAAGCGGCC GCCCAGAGGC TAGGCATGCT GGAAGCCAGT
    ATCCTGCCCC AGGAGGGGCG CTTCCAGGCC CTGGCCGAGA TTGCGGACAT CCTCCATCAG
    GAGCGCTACC ACGGTGGGAC AGAGGTGGCC AGCAGGCAGC TGGAGATCAC CCAGCGCTGG
    GAGCGGCTCC TCCAGCGGCT CCAAGGGCAG AGGAAGCAGA TAGCGGGCAT GCAGGCTGTG
    CTGAGCCTGC TGCAGGAGGT GGAGACTGCC TCCGACCAGC TCAACGAGCT GCAGGTGCTA
    GTCAGCTCCA TGGCCTGTGG GCAGAAGCTG ACAGAGGTAG AGAGGCTGCT GCAGAGGCAT
    GAGCTGCTGG AGGCCCAGAT CTCAGCCCTT GGAGCCCACG TGAGCCATCT GGCCCACCAG
    ACGATGAAGC TGGACTCTTC CATGGGCACT GCTGTGGAGG TGCTGCAGGC CAAGGCCAGG
    GCGCTGGCCC AGCTCTACCA GAGCCTCATG TCTCTTGCTA GGTCCAGGAG GGCCTCACTG
    GAGCAGACGC TGCAGCAGGC AGAGCTCCTG CACAGCTGTG AGGAGGAGGA AGCCTGGTTG
    AGGGAGCATG GACAGCTGGT GGAGGATGCG TCCCCGGGCC CGGATCTCAG CCAGATCGCA
    GCGGCCCTGC AGAAACACAA GGCCCTGGAA GCAGAGCTCC ATCGCCACCA GGCCGTGTGT
    GCTGATCTCG TGCAGAGGGG ATGCAAACTG GGAGCCTGCG GGCCCCCAAC GGGGCCGGAT
    CCCTGGGAGC GGGCTGAGGT GGTGCAGGGC ATGTGGCAGC GGCTCTGGGC CCGGATGGAT
    GAGCAGGGCA TGAGGCTGCA GGCGGCCCTG CTCGTCCAGC AGTACTTCGT GGACGTGGCG
    GAGGCAGACT CGTGGCTTCA GGAGCAGCGG TCTACACTGG AGAGTGCATC CTTTGGACAG
    GACCAGGTGG CCACCGAGGC CCTACTGCGG CAACACCTGC ATCTGGAACG CTCTGTGCGC
    GCCTTCGGGA CCGAGCTGCG TCGATTAGAT GAGCAGGCAC AGGTGGCCAC AGCCCAGGCA
    GCACTCCTGA GGGAGCCCCC AGAAGGCTGG AGCAGAACTG ATGGAGATGT CACCAGGAGA
    CCTCAGGATG GGACCCAAAT TTTAGCACTG CGGGGCAGCC CTCAGATCAA GACAAGCTTC
    ACCTACAGAG GCACGCAATT CACCCAGCTC CCAGGAGAGG CCCAGCAGCC CCCACACCAG
    ATGGACCCCA ACACCCCAGG AGGCTGCCAG GCCACAGTGG CCCTCAGGCA CATCATGGGA
    CAGGAAGCTC AGGTGGTGTC TGCCCTGAGC TCTCCGAAGA TAGGCCCAAG AAACCCAGGG
    GCCTGGCGTG AGGTTTTCTG CCACTCTGGT CTGTGGCGCA CCCAGAAGAT GACCCTCCCA
    GATGAGCCTG ACCCTGGCTT TGACCCCAAC ACCATCCTCC AGACACAGGA CCGCTTGAAC
    CAGGACTATG AGGGCCTGAG GGCCCAGGCA GAGCGCCGCA GGGCCCAGCT GGAGGAGGCC
    GTGGCCCTGT TTGACTTCTA TGGCTCATGT GGGGAGCTGC AGTCCTGGCT GACCAAACAG
    ACAACGCTGT TGCAAATGCT GCAGCCCGAG GCCAACAACT TGGAAGTTAT GCAGCTCAAG
    TATGAGAACT TCCTCACTGC CCTGGCTGTG GGAAGAGGCC TCTGGACTGA GGTTAACAGC
    TCCGCTGAGC AGCTGAAGCA GAGGTGTCCT GGAGGTTCCC CCCAAATCCA GCTACAGCAG
    GAGGAACTGA GCCAGAGGTG GGAGCAGCTG GAGGCCCTGA AGAAAGAGAA GGAAACGCAG
    CTGGTGTGCA CAACGCACGT GTGCAGTTTT CTGCAGGAGT GTGGATCCAC ACGGGTCCAG
    CTGCAAAACC TGGTACTCCA GCTAGAAAGC TTGGAGCTGG GGAACTCAGA GGATGGCCAC
    CATGTTCTGC GAGTGGGCCA GCAGAAGATG CTGGTGCTGG AGAAATACAT TCGTCACCTG
    CAAAGGGCAG CTAGCAAGGT TGAGGAGATG GCCCCGGCCG AGAGCCCGTC CCTGCAGGCA
    CATGTGGAGA CACTGCAGGG GCTGCTGGAG CGAGTGCGGG AGCAAGTAGC CCAGCGGGCC
    AAGGCCCGGG CCCACCGGAG CTTCCTGCAG GAGAGCGAGC GGCTGCTGCT GTGTGCAGAC
    AGTATCCAGG CCAAGCTCCA CAGCGAGGAG GAGGCGGTGG ATGTGGCCTC AGCCCAGCGG
    CTGCTGGGGA AGCACCGAGA CCTGCTGGAG GAGATCCACC TGCAGCAGGA GAGGCTGCAG
    CAGCTGGAGA CTCAGGGCCA GCCCATGGCA GCCTCGGGCT CCCCAGACTT CCGAGAGGCA
    GCTAGTACCC TGAGGCTCCT GGGCCACCAA GGCCAGGAGT TGAAGGCCGC CTGGGAGCAA
    AGACAGCAGC AACTGCAGGA GGGGCTGGAA TTGCAGAAGT TTGGTCGCGA CGTGGATGGT
    TTCACTGCCT CCTGTGCCAA CCATGAGGCC TTCCTACAGC TGGACGTCCT CCAGGAGGGT
    GTGGTGGAGG CCCAGAGTCT GATGCAGAAG CACCAGGAGT GTGGGCGGCT GCTGGATGCC
    CTTGGCTCTA GAGCAGAGGA TCTACGGGCA CGTGGCAAGA AGCTGGCTCA GAGCCGACAC
    CCCGCTGCAC ACAAGGTCAG AGAACAACTG CAGAGCATCC AGGAGCAGTG GACCAGGGTC
    CAGGAGAGGA GTGAACAGAG AAGGAGGCAG CTGCTGGCCT CCCTCCAGCT CCAGGAATGG
    AAGCAGGACG TGGTGGAGGT GATGCTGTGG ATGGAGGAGA AGGGGCGGGT GTTGGTGGAC
    GAGCCATCTC GAGAGCCCAG CAACATCATG AGGAAACTCA GGCGGCACGA GGCAGCCACG
    TGTGAGCTGG CAGCCACCCG AGGGCACGTG GAGGGCCTGC AGCAGGTCGG GAGAGAGCTG
    CTGAGCAGCA GGCCCCATGC CCAGGAGGAC GTGCAGGCCA GGCTTCAGGG CCTAAGTAGC
    AAATGGGAAG ATTTGAGATG CAAGATGGCA GAGCGTGGGA AGCAGCTCCA GCAGGCTCGG
    CAGCAGGACC AGCTCCTGCG GCTGCTACAG GAAGCCAAGG AGAAGATGGA GCAGCTAGAG
    GGGACTGTGC AGAGGGCAGA GGTGGGACAG GACCTGGGCT CCAGCCAGGG GCTGCAGAAA
    CAGCATTGCC AGCTGGAGGC CGAGAGCCAG GCACTGGCCA GCAAGATGGC TGCCCTTGTC
    CCCGAGGCCC GTCAGGCAAT CAGTTCCCAG GCCATCATGG AGGAGACCCA AAAATACCTC
    CAGAGGTTCA AGGCCCTGCA GGGACATCTG GCCAGCCGGC GACGGCAGCT GCAGGCCTCG
    GTTGAGCTGT ACCACTTCCA CCACCTGAGC AACGCGGAGC TCATGTGGGT GGCAGAGCAC
    ATGCCCACGC CCAGCGCCAG CTCTGCTAAG TGCCCAGATG GTGCCCACCG CCTTCTCCAC
    AAGCACAAGG AACTCCGGGT GCAGGTGAGA GCCCACCAGG GACAGGTGCG ATGGGTGCTG
    GGCTCCGGAC GATACCTCGC AGCCTCCGGG CACCCCCAGG CCCAGCACAT CATGGAGCGG
    TGCCAGGAGC TGGAAGGCCG CTGGGCGGAT CTGGAGCAGG CGTGTGAGGC ACAGGCCCGG
    CACCTGCAGC AGGCTGCTGC CCTCCAGGAG TGTTTCCTGG ACGCATCAGA GCTGGAGGAC
    TGGGTGGAGG AGAAGTGGCC GCTGGTGAGC AGCCAGGACT ATGGTGGGGA TGAGACAGCC
    ACCATCAGGC TCATTAAGAA GCACCAGGCC CTGCAACAGG AACTGGCTCG TTGTTGGAAC
    TCCGTGGAGG AGCTTGACCG GAGAGCCCAA ACCCTCACTG GCCCCGAGGG CCCTGCGCAG
    CTGGGCGTGG TGCAGAAGAG GCTCCGGGAG CGGCTGCAGG CCCTGCAGGA GCTGGCAGCC
    ACACGGGACC GGGAGCTGGA GGGGACCCTG AAGCTGCATG AGTTCATGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC CAGCCAGAAG CAAGTGGCTG GAAGAGGAGA GAGCCTTGGG
    GAAGACTATG AGGACGTCCT GCATCTCTGC ACCAAGTTTT TCAGGTTTCA GCGCACAGTA
    GAGATGGGTG GCCAGCGGGT GGCAAGCTGC CAGCAGCTGG CAGAGAAGCT GCTGGAACGA
    GGGCACAGAG CAGCCCCCAG GGCCCATCAG ATGCAGCAGG ATCTGCAGGC TGCCTGGTCA
    GAGCTATGGG AGCTGACCCA GGCCCGAGGC CGCCTGCTCC GAGACGCTGA GGCCACCCTT
    AAGGTTCACC GAGACATGTT GGAAGCCCTC ACCCAGGTCC AGGAGAAAAC CACCAGCCTC
    CCCTGTGACG TGGCCCAGGA CCTGCGTGGG CTAGAGCCCC AGCTGAGGAG ACATGAGGGG
    CTGGAACGTG AACTCATGGG CATTGACCAG CAGCTGCAGG AGCTGCTGGA GATGGGAGGC
    ACGGTGCAGA AGCTAGGGCC CGGGCCGCAG GCCCATGCAG TACAGCAGAG GCAGCAGGCC
    CTGGTGCAGG CCTGGGAGAC CCTGAAGCTG CGAGCAGAGC AGCGCCGGGC CCAGCTCGAG
    CGGGCATGGC TCCTGGCCCG CTTCCACATG GCGGTGCGGG ACTACACCTC CTGGGTGGCC
    AGCGTGTGGC AGGACCTGCA AGTGGAGGCA GGCTCCCAGG AGCCCGGGAG CGGCCCACTG
    AAGCTCAGCG CCCACCAACA GCTTCGGGCC ATGCTGGAGG CCCGGGAGGA GCTGTACCAG
    CAGGCCGCCC AGCTGGGCCA GCAGGCGCTT CTGGCTGCAG GGACATGCAT CACGGAGGTC
    CGGGACAGGC TGCAAGCCCT GCGGGACAAG CGGGAGCGGG TATTCCAGGC CTGGGAGCAG
    AAGCAGGAGA GGCTGCAGGC CATGGGCCAG GAGCAGCTCT TCCTCAGGAA GTGTGGCCGC
    CTGGACGAGA TCCTGAAGGC CCGGGAGGTC CTCCTGAAAA CCAGTGCCTT GGGGAGCTCA
    GTGGAAGAGG TAGAGCAGTT AATCCGCAAG CATGAGACCT TCCAGAAGGT GCTGGCTGCA
    CAGGAGGAGA AGGAGGCAGC CCTGTGTGAG CAAGTGAAGA TGCTCGGAGG CCCCAGGGCA
    CAGGATCTGC TTTGCACAGT GCTCGAGCGC CGGGCTCGGG TGAAGGAACT GGTGGAGAGT
    CGGCGACACG CCTTGCACAC CTCCCTGATG GTGGCTGCCT TCATCAGGGC CGCCACCCAG
    GCCAAGGACT GGATCCAGGA GCAACTCCAG CAGCTGAAGG AGCCAGTCCC TTCTGGGGAC
    CTGAAAGCTA AGTTGAGACA CCTGCAGAAA CACCAGGCCT TTGAGGCTGA GGTCCAGGCC
    CATGAGGAAA TCATAACCTC TGTTGTCAAG GAGGGCGAGG CTCTCCTAGC ACATAGCCAC
    CCTCTGGTGG GAGAGGTCTC CCAGAGGCTG CGGGAGCTGC AGGAGCACTG GGAGAAGTTG
    AGGCAGGCAG TGGCTCTCCG GGGCGAGGAC CTGGAGGACA AGCGGAACAT CCTGGAGTTC
    CTGCAGAGAG TGGACACTGC AGAGGCCTGG ATCCAGGAGA TGGAGGTGAT GGTGAATGTC
    AGCGACCTGG GCCAGGACCG TGAGCACTGC CAGCAGCTCC ACAGGCAGCT CCGCAAGCTC
    CGAGGAGTCT GGGCCGGGGA CACGGTGGAT GATGCCCGCA TCAGAAGCAT CAGTGACTTC
    TCACCACAGC TCAAGACTCA GGACACAGAG CAACTCAAGA CCATCAGGCA GCGGCAGCAG
    CAGCTCAACA ACAGGCCGGT CCCAGAGCAA GAAGCAGTGG GGCTGGGAGA GGGGGCTGAG
    GGTCCAACCC AGACTCAGCC TTACACCCTC TGCCTCAGGT GGAACAGTTT CCATGGCAAC
    CTGCTCAGAT ACCAGCAGCA GCTCGAAGTG GCCCTGGAAA TACACACGTT GTCCCAAAAG
    CTTGATGACA TCACTCAGCA GGTCAGGAAG AAGGCCGCCC TGGTCCAGGC CCTGGACTGT
    GGGAAAGATC TGGACAATGT GCAGAGGCTG AAGCAGAAAC ACAAGGAGCT GGAGCAGGAA
    ATGGGCCTCC TCCAGGCCCA GGTGGAGCCT CTAGATCGTG AAGCAGGCCG AGTCTGCCAG
    AGAAGCCCTG AGGTGGCCCA CGGCCTCAGC CACCAGCAGC AGGAGATGAT GGACAGCTGG
    CGGCAGCTCC AGAGCAGGAT CCAGAAGTGG AAGGAGTCGC TGGACGCCTT GCAGCAAGCT
    CAGGAGCTGC AGGCAGTGCT TCGGGAACTG CTGGTCTGGG CCCAGAGGCT GCGAGCTCAG
    CTGGACTCCC GGAGAAGCCC CGGCAGCCTC GTGGAGGCCC AGCACATGTT GGAGGAACAC
    CAGGAACTCA AAGCTGAGCT GGATTCCCGA ATGGACAGCA TCAGCCTGGC CCGGAGCACA
    GGGCAGCAAC TGCTTGCTGC GGGGCACCCA TCCACCCCCA ACATTCGCCA GGCCCTGGCT
    GATTTTAATC AGGAGCTGAA CAGCCTGGAA GGGGCCTGGC ATGAGCACCA GCTTCACCTG
    CATCAGGCCC TGGAACTACA GCGGGTTCTG AGCTCTGTGG AGCAGATGGA AAGTTGGCTC
    TCCAGCCTGG AAGCCTGCCC AGCCAGTGAG GGTCTGGGGG ACTGCTTGGC CAACGTGGAG
    ACCCTGCTGT GGAAGCACAG GGTGCGTGAG CAGGACCTGG AGGCTCAGGC GGAGAAGATG
    AGCGCGCTGG AAGCCGCCAG CCGCAGCCTG CTCCAGGGCG GACACCCCGA GGCCCAGGGC
    ACGCTGAGTC GGTGCCAGGC CATGCTTCTG AGGAAAGAGG CACTCCTGGA GCGAGCCAGG
    ACCCGTTGCT GCCAGCTGGA GGAGCTCAGG CAACTGCAGT CTTTCCTGCA GGACTCCTGT
    GAGATGGCCG CCTGGCTGAG GGAGAAGAAC CTGGTGGCCC TGGAAGAGGG CTGGTGGGAC
    CCAGTGGACC TGCAGGCCCA GTTGCAGCAA CAACAGCATC TCCAGGCGGA GCTGGACACC
    CGTGCACACC ACCAACAGAG GCTGCAGATG GAGGGGCAGA GGCTGCTACA GGATGGCCAC
    TTGGCCTCAG AGACCATCCA GGAGCGGCTG CAGGAGCTGA GAAAGCTCTG GGAGGAGCTG
    CAGGCCCAGA GCCAGAGGAG GGCAGCCAAG CTACAGGAGG CCCGTGAGGC CCTGCGTCTG
    CGACGGAGCA TGGAGGAACT GGAGAGCTGG CTGGAGCCTG TGGAGCTCAA GCTGAGAGTC
    CCCATCGGGG GCCAGGACCA GCCTGGGCTG GATGAGCTCC TGGGGGATCA GGGGGAGCTG
    GAGGCAGCAG TGGACAGGCA GGTCGGGCGG GCACAGGCCC TGCTGGGCCA AGCCCAGGCC
    CAGGCCTGTG TCCAGGAAGG TCACTGCCTG GCCCGAGACG TGGAAGAGCA GGCCCAGCGG
    CTGCTTCAGA GGTTCGAGAG CCTGCGGGCG CCCCTGCAGG AGCGCAGGAC AGCCCTGGAG
    GCCCGGAGCC TGCTGCTGCA GTTCTTCAGG GACGCAGAAG AGGAGATGGC CTGGGTGCAG
    GAGAAGCTGC TCCTGGTGGC CGCCCGGGAC TGTGGCCAGA GCCCAAGTGC CCTGCGGCGC
    CTGCAGGAGA AGCACCAGAG CCTGGAGAGT GAGATGAGCA GCCACGAGGC TCTAACCCGG
    GCGGTGGTGG GCACAGGGCG CAAGCTGGTA CAGGCTGGGC ACTTCGCTGC CCGCGATGTG
    GCTGCCCGAG TGCAGCAGCT GGAGGACGCC ATGGGCCGCC TGCGGGCAGA GGCTGCACAG
    AGGCGTCGGC GGCTACAGCA GGCTCAGGAG GCCCAGTACT TCCTGACGGA GCTCCTGGAG
    GCAGAATCCT GGCTGGAAGA ACGGGGCTGT GGCCTGGACA TCGAGGATAT GGGTCAGAGT
    GCCGAGGCCA CGCAGGCTTT CCTACGGCAG CTGGAGGCCA CCAGGAGGGA CCTGGAGGGA
    TTTTCCACGC GCATTGAGAG GCTGCAGCAG ACAGCAGCCC TCCTTGAGAG TGGGCAGAAC
    CCAGAAAGCC CCAAGGTGCT TGCCCAGATG CATGCGGTGA GGGACGCCCA CTCAGGGCTG
    CTGCAGAGAG TGGAGAGTAG GGGGCAAGGC CTGAAGGAGC AGCTGCAGCT CCACCAGCTG
    GAGCGGGAAG CCCTGCTCCT GGATGCCTGG CTGGCCAGCA AAGTGGCCAC TGCCGAGTCC
    CAGGACTACG GGCAGGACCT AGAGGCTGTC AAGCTGCTGG AAGAGAAGTT CGATGCTTTC
    AGGAAGGATG TCCAGAGCCT GGGGCAGGCT AGGGTGCAGG CCCTGAGGGA GCGGGCAGGC
    TCCCTGGAAC GAGCAGCACC CAGGTGCTCT CCTCAGATTC AAGCACAGAG GAGTCGCATT
    GAGGCGTCTT GGGAGAGGCT GGATCAGGCA GTGAAAGCCC GCACACAGAA CCTAGCTGCC
    GCCCGTGAGG TCCGAGGCTT GGAGCAGGCA GCAGCTAAGC TCCAGGCCCG GATGCAAAAG
    AAGGCCACCC TGGTAGCTAG AGATGCCTTC AACCTAAGCC TGTCATCAGT GCAGACTCTG
    CAGCAACAGC ACAGATGTCT GGAGAGGGAA CTGGCAGCGA TGGAAAAGGA GGTGGCACAG
    GTGCAGACGG AGGCCTGCCG CCTGGGCCAG CTCTACCCTG CAGCTCAGGA GGGCCTGGCC
    AAGCAGCTGG CCGGGGTGCA GGAGGCCTGG GCCACCCTGA ACGCAAAGGT CCATGAGCAA
    GATCGGCAGC TGGAGAAGGC TGCCGAGGGC CATGCCTTCC TCGGACGCTG TCGGGAATTG
    CTAGCATGGG CCACGGAGAA GCAGGCCCTG GTGTCCTCGG AGGAGCTGGC TGGGGATATA
    GCTCGGGCTG AGGGCCTCCT GGCACTGCAT GAGAAGCTGG GGAGAGAAAT CAAAGAGTAC
    TGCCTTCAAG CCCAGAACGT ACAGCAGGAA GGGCAGCGGC TGGTGGACAG TGGCCACTGC
    ATGTCCCTGG AGGTAACTGG GTGTCTGCAG GATCTGGACA GGCAGCTGCG GGCACTCAGA
    GAGGCCTGGG CCCTGCGCCG GGAACGCTGT GAGGAGAGCT GGCGTCTACA GAAGCTGCGG
    CAAGAGCTGG ACCAGGCCGA GGCCTGGCTG GCCTGCCGGG AGGGCCTCCT GCTGGACCCC
    AACTGCGGGC ACTCCCTGTC TGATGTGGAG TTGCTGCTCT GCAGACACAA GGACTTAGAG
    AAGCTGCTGG CCTCCCAGGA AGAGAAGTTT GCCCGGCTGC AGCAGGAGGC AGAGGTGGAA
    CAGAAGCAGC AGGTGAAGGG GCTGAAACCC TTGAGAAGCT GGTGGCCCAG AGCACAGCTG
    ACTGAGACAG GGGACCAGCA GCCATCTGTC CCCATTGAGT CTTGCATACG CTTCACCGCC
    GTCTCCCCGG AGTGCACAGC GGGGACCCTG GGGGATGCAA AGGGTGTCCC CACCACGGAG
    GGAATTTTGG AACTTAAGCA GCAGCTCCTG CCTGGGAGAA GGCAGAACAC GGCTTCTACA
    GCCTCCCTCG ACCTCGTAGG AGTGCAGCGT GAGAAGCCGG GAGATCACCA TGATGGAAAA
    CACACTTTTT CCTTAAGGCT AACTGCAGGA GAGACCCTGT CTGCAGCGCC ATCTGCAGGA
    CAGACTGGGA GCTGGCGGGG CACCCTGAGC AGCTGGACAG CCCCCAGTCT GAGCCCAGAG
    CTCAAAGCCA GACCCTCAGG CTTCCTGGCT GAGCGCAATG CTGAGAGGGT ACCCGGCATG
    CCAGCTCCAC TCAGACTCCA CAGCGGTACC CCGCCCGTCT GGCTGCAGGA GACACAAGTG
    TGCACCTATA GCTTCCCGCA GCCCGTGCGG CTCCCACAGA CGGCTGGCCT CGCAGAGCAA
    ACTGCAGAAG GCTCCCACCG ACTGTCCTGC TCAGGCCAGA TCCATGGTCT GGGCCTCGAG
    GCAAAAGCAG AGCTGGCACC AAGCCTCAGG GCTACAAGCG AGGACACCCT GCTATCTGGC
    CGTTCAGGAC CAACTTCTGG AACAGCTGGT TCCTCATCCA GGGATGGGCC CCACCGAGGT
    CAGAGCCTGC TCAAGTCACA CCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320535.1
    CDS53..11539
    Translation

    Target ORF information:

    RefSeq Version XM_015465049.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    ATGGACTCTG CGTACGAGAT GGGCCACATC CGCAAGCTGC AGGCGCGGCA CATGCAGATG 
    CAGGAGAAGA CCTTCACCAA GTGGATCAAC AATATCTTCC AGCATGGCCG GGTGGGCATC
    GAGATCCAGA ACCTATGCAC AGAGCTGGCC GACGGCACCC ACCTGCTGCG GCTCCTGGAG
    CTCATCTCAG GGGAGGCCCT GCCACCCCCC AACAGGGGCC GCATGCGCGT CCATTTTCTG
    GAGAACAATA GCCGAGCTCT GGCTTTCCTC AGGGCCAAGG TGCCAATACC CCTCATTGGG
    CCAGAGAACA TCGTGGATGG AGACCAGACG CTCATCCTGG GACTCATCTG GGTCATCATT
    CTGCGTTTCC AGATTTCCGA CATCTGCCTG GACAAGGAGG AGTTTGGGGT CAGCGCAGCC
    CTGCTGTCTG CCAGGGAAGC TCTGCTGGTC TGGTGCCAGC GGAAGACTGC CGGCTATGCC
    AACATCAACA TCACGGACTT CTCCCGCAGC TGGAGTGACG GGCTCGGCTT CAGTGCCCTC
    ATCCATGCCC ACAGGCCAGA CCTGCTTGAC TACAGCTCCC TGCGTCCCGA CCGCCCGCTG
    CACAACCTCC GCTGTGCTTT CCACGTAGCT GAGCAGGAGC TGGGCATTGC TCAGCTGCTG
    GATCCAGAGG ATGTGGCAGC CCTACAGCCT GACGAGCGCT CCATAATGAC CTACGTCTCC
    CTCTACTACC ACCACTTCTC CCGCCTGCAC CAGGAGCAGA CCGTCCAGAG GAGACTCACA
    AAGATCCTGC TTCAGCTGCA GGAGTCAGAG GCGCTGCAGA CCCAGTACAA GGAGCTGGTG
    GCCGACCTGC TGTGCTGGAT TGCAGAGAAG CAGGTGCAGC TGGAGGCGCG GGATTTCCCA
    GACAACCTGC CCGCCATGCG CCAGCTACTG GTGGCCTTTG CCTCCTTCCG CAGCCAGGAG
    AAGCCACCGA GACTGCAGCA GCGTGGGGCC ACGGAGGCCC TGCTCTTTCG GCTGCAGACA
    GTCCTCCAAG CCCAGAACCG CAGGCCCTTC CTGCCACCTG AGGGCCTGGG CCCTGCAGAG
    CTGTCCCGGT GCTGGACAAG CCTGGAGCGG GCGGAGGCCT CACGGAGCCG GGCCCTGCAG
    CAGAGGCTCC TACAGCTAGA GCGGTTGGAA ACCCTGGCCC GGCGTTTCCA GCGTAAGGCA
    GCCCTCCGGG AGAGCTTCCT GACAGACACG GAGCAGGTGC TAGACCGGGC CGCAGCCCCG
    CTGGCCAGCC GGGCCATGCT GGAAGCGGCC GCCCAGAGGC TAGGCATGCT GGAAGCCAGT
    ATCCTGCCCC AGGAGGGGCG CTTCCAGGCC CTGGCCGAGA TTGCGGACAT CCTCCATCAG
    GAGCGCTACC ACGGTGGGAC AGAGGTGGCC AGCAGGCAGC TGGAGATCAC CCAGCGCTGG
    GAGCGGCTCC TCCAGCGGCT CCAAGGGCAG AGGAAGCAGA TAGCGGGCAT GCAGGCTGTG
    CTGAGCCTGC TGCAGGAGGT GGAGACTGCC TCCGACCAGC TCAACGAGCT GCAGGTGCTA
    GTCAGCTCCA TGGCCTGTGG GCAGAAGCTG ACAGAGGTAG AGAGGCTGCT GCAGAGGCAT
    GAGCTGCTGG AGGCCCAGAT CTCAGCCCTT GGAGCCCACG TGAGCCATCT GGCCCACCAG
    ACGATGAAGC TGGACTCTTC CATGGGCACT GCTGTGGAGG TGCTGCAGGC CAAGGCCAGG
    GCGCTGGCCC AGCTCTACCA GAGCCTCATG TCTCTTGCTA GGTCCAGGAG GGCCTCACTG
    GAGCAGACGC TGCAGCAGGC AGAGCTCCTG CACAGCTGTG AGGAGGAGGA AGCCTGGTTG
    AGGGAGCATG GACAGCTGGT GGAGGATGCG TCCCCGGGCC CGGATCTCAG CCAGATCGCA
    GCGGCCCTGC AGAAACACAA GGCCCTGGAA GCAGAGCTCC ATCGCCACCA GGCCGTGTGT
    GCTGATCTCG TGCAGAGGGG ATGCAAACTG GGAGCCTGCG GGCCCCCAAC GGGGCCGGAT
    CCCTGGGAGC GGGCTGAGGT GGTGCAGGGC ATGTGGCAGC GGCTCTGGGC CCGGATGGAT
    GAGCAGGGCA TGAGGCTGCA GGCGGCCCTG CTCGTCCAGC AGTACTTCGT GGACGTGGCG
    GAGGCAGACT CGTGGCTTCA GGAGCAGCGG TCTACACTGG AGAGTGCATC CTTTGGACAG
    GACCAGGTGG CCACCGAGGC CCTACTGCGG CAACACCTGC ATCTGGAACG CTCTGTGCGC
    GCCTTCGGGA CCGAGCTGCG TCGATTAGAT GAGCAGGCAC AGGTGGCCAC AGCCCAGGCA
    GCACTCCTGA GGGAGCCCCC AGAAGGCTGG AGCAGAACTG ATGGAGATGT CACCAGGAGA
    CCTCAGGATG GGACCCAAAT TTTAGCACTG CGGGGCAGCC CTCAGATCAA GACAAGCTTC
    ACCTACAGAG GCACGCAATT CACCCAGCTC CCAGGAGAGG CCCAGCAGCC CCCACACCAG
    ATGGACCCCA ACACCCCAGG AGGCTGCCAG GCCACAGTGG CCCTCAGGCA CATCATGGGA
    CAGGAAGCTC AGGTGGTGTC TGCCCTGAGC TCTCCGAAGA TAGGCCCAAG AAACCCAGGG
    GCCTGGCGTG AGGTTTTCTG CCACTCTGGT CTGTGGCGCA CCCAGAAGAT GACCCTCCCA
    GATGAGCCTG ACCCTGGCTT TGACCCCAAC ACCATCCTCC AGACACAGGA CCGCTTGAAC
    CAGGACTATG AGGGCCTGAG GGCCCAGGCA GAGCGCCGCA GGGCCCAGCT GGAGGAGGCC
    GTGGCCCTGT TTGACTTCTA TGGCTCATGT GGGGAGCTGC AGTCCTGGCT GACCAAACAG
    ACAACGCTGT TGCAAATGCT GCAGCCCGAG GCCAACAACT TGGAAGTTAT GCAGCTCAAG
    TATGAGAACT TCCTCACTGC CCTGGCTGTG GGAAGAGGCC TCTGGACTGA GGTTAACAGC
    TCCGCTGAGC AGCTGAAGCA GAGGTGTCCT GGAGGTTCCC CCCAAATCCA GCTACAGCAG
    GAGGAACTGA GCCAGAGGTG GGAGCAGCTG GAGGCCCTGA AGAAAGAGAA GGAAACGCAG
    CTGGTGTGCA CAACGCACGT GTGCAGTTTT CTGCAGGAGT GTGGATCCAC ACGGGTCCAG
    CTGCAAAACC TGGTACTCCA GCTAGAAAGC TTGGAGCTGG GGAACTCAGA GGATGGCCAC
    CATGTTCTGC GAGTGGGCCA GCAGAAGATG CTGGTGCTGG AGAAATACAT TCGTCACCTG
    CAAAGGGCAG CTAGCAAGGT TGAGGAGATG GCCCCGGCCG AGAGCCCGTC CCTGCAGGCA
    CATGTGGAGA CACTGCAGGG GCTGCTGGAG CGAGTGCGGG AGCAAGTAGC CCAGCGGGCC
    AAGGCCCGGG CCCACCGGAG CTTCCTGCAG GAGAGCGAGC GGCTGCTGCT GTGTGCAGAC
    AGTATCCAGG CCAAGCTCCA CAGCGAGGAG GAGGCGGTGG ATGTGGCCTC AGCCCAGCGG
    CTGCTGGGGA AGCACCGAGA CCTGCTGGAG GAGATCCACC TGCAGCAGGA GAGGCTGCAG
    CAGCTGGAGA CTCAGGGCCA GCCCATGGCA GCCTCGGGCT CCCCAGACTT CCGAGAGGCA
    GCTAGTACCC TGAGGCTCCT GGGCCACCAA GGCCAGGAGT TGAAGGCCGC CTGGGAGCAA
    AGACAGCAGC AACTGCAGGA GGGGCTGGAA TTGCAGAAGT TTGGTCGCGA CGTGGATGGT
    TTCACTGCCT CCTGTGCCAA CCATGAGGCC TTCCTACAGC TGGACGTCCT CCAGGAGGGT
    GTGGTGGAGG CCCAGAGTCT GATGCAGAAG CACCAGGAGT GTGGGCGGCT GCTGGATGCC
    CTTGGCTCTA GAGCAGAGGA TCTACGGGCA CGTGGCAAGA AGCTGGCTCA GAGCCGACAC
    CCCGCTGCAC ACAAGGTCAG AGAACAACTG CAGAGCATCC AGGAGCAGTG GACCAGGGTC
    CAGGAGAGGA GTGAACAGAG AAGGAGGCAG CTGCTGGCCT CCCTCCAGCT CCAGGAATGG
    AAGCAGGACG TGGTGGAGGT GATGCTGTGG ATGGAGGAGA AGGGGCGGGT GTTGGTGGAC
    GAGCCATCTC GAGAGCCCAG CAACATCATG AGGAAACTCA GGCGGCACGA GGCAGCCACG
    TGTGAGCTGG CAGCCACCCG AGGGCACGTG GAGGGCCTGC AGCAGGTCGG GAGAGAGCTG
    CTGAGCAGCA GGCCCCATGC CCAGGAGGAC GTGCAGGCCA GGCTTCAGGG CCTAAGTAGC
    AAATGGGAAG ATTTGAGATG CAAGATGGCA GAGCGTGGGA AGCAGCTCCA GCAGGCTCGG
    CAGCAGGACC AGCTCCTGCG GCTGCTACAG GAAGCCAAGG AGAAGATGGA GCAGCTAGAG
    GGGACTGTGC AGAGGGCAGA GGTGGGACAG GACCTGGGCT CCAGCCAGGG GCTGCAGAAA
    CAGCATTGCC AGCTGGAGGC CGAGAGCCAG GCACTGGCCA GCAAGATGGC TGCCCTTGTC
    CCCGAGGCCC GTCAGGCAAT CAGTTCCCAG GCCATCATGG AGGAGACCCA AAAATACCTC
    CAGAGGTTCA AGGCCCTGCA GGGACATCTG GCCAGCCGGC GACGGCAGCT GCAGGCCTCG
    GTTGAGCTGT ACCACTTCCA CCACCTGAGC AACGCGGAGC TCATGTGGGT GGCAGAGCAC
    ATGCCCACGC CCAGCGCCAG CTCTGCTAAG TGCCCAGATG GTGCCCACCG CCTTCTCCAC
    AAGCACAAGG AACTCCGGGT GCAGGTGAGA GCCCACCAGG GACAGGTGCG ATGGGTGCTG
    GGCTCCGGAC GATACCTCGC AGCCTCCGGG CACCCCCAGG CCCAGCACAT CATGGAGCGG
    TGCCAGGAGC TGGAAGGCCG CTGGGCGGAT CTGGAGCAGG CGTGTGAGGC ACAGGCCCGG
    CACCTGCAGC AGGCTGCTGC CCTCCAGGAG TGTTTCCTGG ACGCATCAGA GCTGGAGGAC
    TGGGTGGAGG AGAAGTGGCC GCTGGTGAGC AGCCAGGACT ATGGTGGGGA TGAGACAGCC
    ACCATCAGGC TCATTAAGAA GCACCAGGCC CTGCAACAGG AACTGGCTCG TTGTTGGAAC
    TCCGTGGAGG AGCTTGACCG GAGAGCCCAA ACCCTCACTG GCCCCGAGGG CCCTGCGCAG
    CTGGGCGTGG TGCAGAAGAG GCTCCGGGAG CGGCTGCAGG CCCTGCAGGA GCTGGCAGCC
    ACACGGGACC GGGAGCTGGA GGGGACCCTG AAGCTGCATG AGTTCATGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC CAGCCAGAAG CAAGTGGCTG GAAGAGGAGA GAGCCTTGGG
    GAAGACTATG AGGACGTCCT GCATCTCTGC ACCAAGTTTT TCAGGTTTCA GCGCACAGTA
    GAGATGGGTG GCCAGCGGGT GGCAAGCTGC CAGCAGCTGG CAGAGAAGCT GCTGGAACGA
    GGGCACAGAG CAGCCCCCAG GGCCCATCAG ATGCAGCAGG ATCTGCAGGC TGCCTGGTCA
    GAGCTATGGG AGCTGACCCA GGCCCGAGGC CGCCTGCTCC GAGACGCTGA GGCCACCCTT
    AAGGTTCACC GAGACATGTT GGAAGCCCTC ACCCAGGTCC AGGAGAAAAC CACCAGCCTC
    CCCTGTGACG TGGCCCAGGA CCTGCGTGGG CTAGAGCCCC AGCTGAGGAG ACATGAGGGG
    CTGGAACGTG AACTCATGGG CATTGACCAG CAGCTGCAGG AGCTGCTGGA GATGGGAGGC
    ACGGTGCAGA AGCTAGGGCC CGGGCCGCAG GCCCATGCAG TACAGCAGAG GCAGCAGGCC
    CTGGTGCAGG CCTGGGAGAC CCTGAAGCTG CGAGCAGAGC AGCGCCGGGC CCAGCTCGAG
    CGGGCATGGC TCCTGGCCCG CTTCCACATG GCGGTGCGGG ACTACACCTC CTGGGTGGCC
    AGCGTGTGGC AGGACCTGCA AGTGGAGGCA GGCTCCCAGG AGCCCGGGAG CGGCCCACTG
    AAGCTCAGCG CCCACCAACA GCTTCGGGCC ATGCTGGAGG CCCGGGAGGA GCTGTACCAG
    CAGGCCGCCC AGCTGGGCCA GCAGGCGCTT CTGGCTGCAG GGACATGCAT CACGGAGGTC
    CGGGACAGGC TGCAAGCCCT GCGGGACAAG CGGGAGCGGG TATTCCAGGC CTGGGAGCAG
    AAGCAGGAGA GGCTGCAGGC CATGGGCCAG GAGCAGCTCT TCCTCAGGAA GTGTGGCCGC
    CTGGACGAGA TCCTGAAGGC CCGGGAGGTC CTCCTGAAAA CCAGTGCCTT GGGGAGCTCA
    GTGGAAGAGG TAGAGCAGTT AATCCGCAAG CATGAGACCT TCCAGAAGGT GCTGGCTGCA
    CAGGAGGAGA AGGAGGCAGC CCTGTGTGAG CAAGTGAAGA TGCTCGGAGG CCCCAGGGCA
    CAGGATCTGC TTTGCACAGT GCTCGAGCGC CGGGCTCGGG TGAAGGAACT GGTGGAGAGT
    CGGCGACACG CCTTGCACAC CTCCCTGATG GTGGCTGCCT TCATCAGGGC CGCCACCCAG
    GCCAAGGACT GGATCCAGGA GCAACTCCAG CAGCTGAAGG AGCCAGTCCC TTCTGGGGAC
    CTGAAAGCTA AGTTGAGACA CCTGCAGAAA CACCAGGCCT TTGAGGCTGA GGTCCAGGCC
    CATGAGGAAA TCATAACCTC TGTTGTCAAG GAGGGCGAGG CTCTCCTAGC ACATAGCCAC
    CCTCTGGTGG GAGAGGTCTC CCAGAGGCTG CGGGAGCTGC AGGAGCACTG GGAGAAGTTG
    AGGCAGGCAG TGGCTCTCCG GGGCGAGGAC CTGGAGGACA AGCGGAACAT CCTGGAGTTC
    CTGCAGAGAG TGGACACTGC AGAGGCCTGG ATCCAGGAGA TGGAGGTGAT GGTGAATGTC
    AGCGACCTGG GCCAGGACCG TGAGCACTGC CAGCAGCTCC ACAGGCAGCT CCGCAAGCTC
    CGAGGAGTCT GGGCCGGGGA CACGGTGGAT GATGCCCGCA TCAGAAGCAT CAGTGACTTC
    TCACCACAGC TCAAGACTCA GGACACAGAG CAACTCAAGA CCATCAGGCA GCGGCAGCAG
    CAGCTCAACA ACAGGCCGGT CCCAGAGCAA GAAGCAGTGG GGCTGGGAGA GGGGGCTGAG
    GGTCCAACCC AGACTCAGCC TTACACCCTC TGCCTCAGGT GGAACAGTTT CCATGGCAAC
    CTGCTCAGAT ACCAGCAGCA GCTCGAAGTG GCCCTGGAAA TACACACGTT GTCCCAAAAG
    CTTGATGACA TCACTCAGCA GGTCAGGAAG AAGGCCGCCC TGGTCCAGGC CCTGGACTGT
    GGGAAAGATC TGGACAATGT GCAGAGGCTG AAGCAGAAAC ACAAGGAGCT GGAGCAGGAA
    ATGGGCCTCC TCCAGGCCCA GGTGGAGCCT CTAGATCGTG AAGCAGGCCG AGTCTGCCAG
    AGAAGCCCTG AGGTGGCCCA CGGCCTCAGC CACCAGCAGC AGGAGATGAT GGACAGCTGG
    CGGCAGCTCC AGAGCAGGAT CCAGAAGTGG AAGGAGTCGC TGGACGCCTT GCAGCAAGCT
    CAGGAGCTGC AGGCAGTGCT TCGGGAACTG CTGGTCTGGG CCCAGAGGCT GCGAGCTCAG
    CTGGACTCCC GGAGAAGCCC CGGCAGCCTC GTGGAGGCCC AGCACATGTT GGAGGAACAC
    CAGGAACTCA AAGCTGAGCT GGATTCCCGA ATGGACAGCA TCAGCCTGGC CCGGAGCACA
    GGGCAGCAAC TGCTTGCTGC GGGGCACCCA TCCACCCCCA ACATTCGCCA GGCCCTGGCT
    GATTTTAATC AGGAGCTGAA CAGCCTGGAA GGGGCCTGGC ATGAGCACCA GCTTCACCTG
    CATCAGGCCC TGGAACTACA GCGGGTTCTG AGCTCTGTGG AGCAGATGGA AAGTTGGCTC
    TCCAGCCTGG AAGCCTGCCC AGCCAGTGAG GGTCTGGGGG ACTGCTTGGC CAACGTGGAG
    ACCCTGCTGT GGAAGCACAG GGTGCGTGAG CAGGACCTGG AGGCTCAGGC GGAGAAGATG
    AGCGCGCTGG AAGCCGCCAG CCGCAGCCTG CTCCAGGGCG GACACCCCGA GGCCCAGGGC
    ACGCTGAGTC GGTGCCAGGC CATGCTTCTG AGGAAAGAGG CACTCCTGGA GCGAGCCAGG
    ACCCGTTGCT GCCAGCTGGA GGAGCTCAGG CAACTGCAGT CTTTCCTGCA GGACTCCTGT
    GAGATGGCCG CCTGGCTGAG GGAGAAGAAC CTGGTGGCCC TGGAAGAGGG CTGGTGGGAC
    CCAGTGGACC TGCAGGCCCA GTTGCAGCAA CAACAGCATC TCCAGGCGGA GCTGGACACC
    CGTGCACACC ACCAACAGAG GCTGCAGATG GAGGGGCAGA GGCTGCTACA GGATGGCCAC
    TTGGCCTCAG AGACCATCCA GGAGCGGCTG CAGGAGCTGA GAAAGCTCTG GGAGGAGCTG
    CAGGCCCAGA GCCAGAGGAG GGCAGCCAAG CTACAGGAGG CCCGTGAGGC CCTGCGTCTG
    CGACGGAGCA TGGAGGAACT GGAGAGCTGG CTGGAGCCTG TGGAGCTCAA GCTGAGAGTC
    CCCATCGGGG GCCAGGACCA GCCTGGGCTG GATGAGCTCC TGGGGGATCA GGGGGAGCTG
    GAGGCAGCAG TGGACAGGCA GGTCGGGCGG GCACAGGCCC TGCTGGGCCA AGCCCAGGCC
    CAGGCCTGTG TCCAGGAAGG TCACTGCCTG GCCCGAGACG TGGAAGAGCA GGCCCAGCGG
    CTGCTTCAGA GGTTCGAGAG CCTGCGGGCG CCCCTGCAGG AGCGCAGGAC AGCCCTGGAG
    GCCCGGAGCC TGCTGCTGCA GTTCTTCAGG GACGCAGAAG AGGAGATGGC CTGGGTGCAG
    GAGAAGCTGC TCCTGGTGGC CGCCCGGGAC TGTGGCCAGA GCCCAAGTGC CCTGCGGCGC
    CTGCAGGAGA AGCACCAGAG CCTGGAGAGT GAGATGAGCA GCCACGAGGC TCTAACCCGG
    GCGGTGGTGG GCACAGGGCG CAAGCTGGTA CAGGCTGGGC ACTTCGCTGC CCGCGATGTG
    GCTGCCCGAG TGCAGCAGCT GGAGGACGCC ATGGGCCGCC TGCGGGCAGA GGCTGCACAG
    AGGCGTCGGC GGCTACAGCA GGCTCAGGAG GCCCAGTACT TCCTGACGGA GCTCCTGGAG
    GCAGAATCCT GGCTGGAAGA ACGGGGCTGT GGCCTGGACA TCGAGGATAT GGGTCAGAGT
    GCCGAGGCCA CGCAGGCTTT CCTACGGCAG CTGGAGGCCA CCAGGAGGGA CCTGGAGGGA
    TTTTCCACGC GCATTGAGAG GCTGCAGCAG ACAGCAGCCC TCCTTGAGAG TGGGCAGAAC
    CCAGAAAGCC CCAAGGTGCT TGCCCAGATG CATGCGGTGA GGGACGCCCA CTCAGGGCTG
    CTGCAGAGAG TGGAGAGTAG GGGGCAAGGC CTGAAGGAGC AGCTGCAGCT CCACCAGCTG
    GAGCGGGAAG CCCTGCTCCT GGATGCCTGG CTGGCCAGCA AAGTGGCCAC TGCCGAGTCC
    CAGGACTACG GGCAGGACCT AGAGGCTGTC AAGCTGCTGG AAGAGAAGTT CGATGCTTTC
    AGGAAGGATG TCCAGAGCCT GGGGCAGGCT AGGGTGCAGG CCCTGAGGGA GCGGGCAGGC
    TCCCTGGAAC GAGCAGCACC CAGGTGCTCT CCTCAGATTC AAGCACAGAG GAGTCGCATT
    GAGGCGTCTT GGGAGAGGCT GGATCAGGCA GTGAAAGCCC GCACACAGAA CCTAGCTGCC
    GCCCGTGAGG TCCGAGGCTT GGAGCAGGCA GCAGCTAAGC TCCAGGCCCG GATGCAAAAG
    AAGGCCACCC TGGTAGCTAG AGATGCCTTC AACCTAAGCC TGTCATCAGT GCAGACTCTG
    CAGCAACAGC ACAGATGTCT GGAGAGGGAA CTGGCAGCGA TGGAAAAGGA GGTGGCACAG
    GTGCAGACGG AGGCCTGCCG CCTGGGCCAG CTCTACCCTG CAGCTCAGGA GGGCCTGGCC
    AAGCAGCTGG CCGGGGTGCA GGAGGCCTGG GCCACCCTGA ACGCAAAGGT CCATGAGCAA
    GATCGGCAGC TGGAGAAGGC TGCCGAGGGC CATGCCTTCC TCGGACGCTG TCGGGAATTG
    CTAGCATGGG CCACGGAGAA GCAGGCCCTG GTGTCCTCGG AGGAGCTGGC TGGGGATATA
    GCTCGGGCTG AGGGCCTCCT GGCACTGCAT GAGAAGCTGG GGAGAGAAAT CAAAGAGTAC
    TGCCTTCAAG CCCAGAACGT ACAGCAGGAA GGGCAGCGGC TGGTGGACAG TGGCCACTGC
    ATGTCCCTGG AGGTAACTGG GTGTCTGCAG GATCTGGACA GGCAGCTGCG GGCACTCAGA
    GAGGCCTGGG CCCTGCGCCG GGAACGCTGT GAGGAGAGCT GGCGTCTACA GAAGCTGCGG
    CAAGAGCTGG ACCAGGCCGA GGCCTGGCTG GCCTGCCGGG AGGGCCTCCT GCTGGACCCC
    AACTGCGGGC ACTCCCTGTC TGATGTGGAG TTGCTGCTCT GCAGACACAA GGACTTAGAG
    AAGCTGCTGG CCTCCCAGGA AGAGAAGTTT GCCCGGCTGC AGCAGGAGGC AGAGGTGGAA
    CAGAAGCAGC AGGTGAAGGG GCTGAAACCC TTGAGAAGCT GGTGGCCCAG AGCACAGCTG
    ACTGAGACAG GGGACCAGCA GCCATCTGTC CCCATTGAGT CTTGCATACG CTTCACCGCC
    GTCTCCCCGG AGTGCACAGC GGGGACCCTG GGGGATGCAA AGGGTGTCCC CACCACGGAG
    GGAATTTTGG AACTTAAGCA GCAGCTCCTG CCTGGGAGAA GGCAGAACAC GGCTTCTACA
    GCCTCCCTCG ACCTCGTAGG AGTGCAGCGT GAGAAGCCGG GAGATCACCA TGATGGAAAA
    CACACTTTTT CCTTAAGGCT AACTGCAGGA GAGACCCTGT CTGCAGCGCC ATCTGCAGGA
    CAGACTGGGA GCTGGCGGGG CACCCTGAGC AGCTGGACAG CCCCCAGTCT GAGCCCAGAG
    CTCAAAGCCA GACCCTCAGG CTTCCTGGCT GAGCGCAATG CTGAGAGGGT ACCCGGCATG
    CCAGCTCCAC TCAGACTCCA CAGCGGTACC CCGCCCGTCT GGCTGCAGGA GACACAAGTG
    TGCACCTATA GCTTCCCGCA GCCCGTGCGG CTCCCACAGA CGGCTGGCCT CGCAGAGCAA
    ACTGCAGAAG GCTCCCACCG ACTGTCCTGC TCAGGCCAGA TCCATGGTCT GGGCCTCGAG
    GCAAAAGCAG AGCTGGCACC AAGCCTCAGG GCTACAAGCG AGGACACCCT GCTATCTGGC
    CGTTCAGGAC CAACTTCTGG AACAGCTGGT TCCTCATCCA GGGATGGGCC CCACCGAGGT
    CAGAGCCTGC TCAAGTCACA CCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91679
    Clone ID Related Accession (Same CDS sequence) XM_015465049.1 , XM_015473137.2 , XM_015473137.1
    Accession Version XM_015473137.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1741
    1801
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    1921
    1981
    2041
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    2641
    2701
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    9781
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    10201
    10261
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    10381
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    10501
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    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    ATGGACTCTG CGTACGAGAT GGGCCACATC CGCAAGCTGC AGGCGCGGCA CATGCAGATG 
    CAGGAGAAGA CCTTCACCAA GTGGATCAAC AATATCTTCC AGCATGGCCG GGTGGGCATC
    GAGATCCAGA ACCTATGCAC AGAGCTGGCC GACGGCACCC ACCTGCTGCG GCTCCTGGAG
    CTCATCTCAG GGGAGGCCCT GCCACCCCCC AACAGGGGCC GCATGCGCGT CCATTTTCTG
    GAGAACAATA GCCGAGCTCT GGCTTTCCTC AGGGCCAAGG TGCCAATACC CCTCATTGGG
    CCAGAGAACA TCGTGGATGG AGACCAGACG CTCATCCTGG GACTCATCTG GGTCATCATT
    CTGCGTTTCC AGATTTCCGA CATCTGCCTG GACAAGGAGG AGTTTGGGGT CAGCGCAGCC
    CTGCTGTCTG CCAGGGAAGC TCTGCTGGTC TGGTGCCAGC GGAAGACTGC CGGCTATGCC
    AACATCAACA TCACGGACTT CTCCCGCAGC TGGAGTGACG GGCTCGGCTT CAGTGCCCTC
    ATCCATGCCC ACAGGCCAGA CCTGCTTGAC TACAGCTCCC TGCGTCCCGA CCGCCCGCTG
    CACAACCTCC GCTGTGCTTT CCACGTAGCT GAGCAGGAGC TGGGCATTGC TCAGCTGCTG
    GATCCAGAGG ATGTGGCAGC CCTACAGCCT GACGAGCGCT CCATAATGAC CTACGTCTCC
    CTCTACTACC ACCACTTCTC CCGCCTGCAC CAGGAGCAGA CCGTCCAGAG GAGACTCACA
    AAGATCCTGC TTCAGCTGCA GGAGTCAGAG GCGCTGCAGA CCCAGTACAA GGAGCTGGTG
    GCCGACCTGC TGTGCTGGAT TGCAGAGAAG CAGGTGCAGC TGGAGGCGCG GGATTTCCCA
    GACAACCTGC CCGCCATGCG CCAGCTACTG GTGGCCTTTG CCTCCTTCCG CAGCCAGGAG
    AAGCCACCGA GACTGCAGCA GCGTGGGGCC ACGGAGGCCC TGCTCTTTCG GCTGCAGACA
    GTCCTCCAAG CCCAGAACCG CAGGCCCTTC CTGCCACCTG AGGGCCTGGG CCCTGCAGAG
    CTGTCCCGGT GCTGGACAAG CCTGGAGCGG GCGGAGGCCT CACGGAGCCG GGCCCTGCAG
    CAGAGGCTCC TACAGCTAGA GCGGTTGGAA ACCCTGGCCC GGCGTTTCCA GCGTAAGGCA
    GCCCTCCGGG AGAGCTTCCT GACAGACACG GAGCAGGTGC TAGACCGGGC CGCAGCCCCG
    CTGGCCAGCC GGGCCATGCT GGAAGCGGCC GCCCAGAGGC TAGGCATGCT GGAAGCCAGT
    ATCCTGCCCC AGGAGGGGCG CTTCCAGGCC CTGGCCGAGA TTGCGGACAT CCTCCATCAG
    GAGCGCTACC ACGGTGGGAC AGAGGTGGCC AGCAGGCAGC TGGAGATCAC CCAGCGCTGG
    GAGCGGCTCC TCCAGCGGCT CCAAGGGCAG AGGAAGCAGA TAGCGGGCAT GCAGGCTGTG
    CTGAGCCTGC TGCAGGAGGT GGAGACTGCC TCCGACCAGC TCAACGAGCT GCAGGTGCTA
    GTCAGCTCCA TGGCCTGTGG GCAGAAGCTG ACAGAGGTAG AGAGGCTGCT GCAGAGGCAT
    GAGCTGCTGG AGGCCCAGAT CTCAGCCCTT GGAGCCCACG TGAGCCATCT GGCCCACCAG
    ACGATGAAGC TGGACTCTTC CATGGGCACT GCTGTGGAGG TGCTGCAGGC CAAGGCCAGG
    GCGCTGGCCC AGCTCTACCA GAGCCTCATG TCTCTTGCTA GGTCCAGGAG GGCCTCACTG
    GAGCAGACGC TGCAGCAGGC AGAGCTCCTG CACAGCTGTG AGGAGGAGGA AGCCTGGTTG
    AGGGAGCATG GACAGCTGGT GGAGGATGCG TCCCCGGGCC CGGATCTCAG CCAGATCGCA
    GCGGCCCTGC AGAAACACAA GGCCCTGGAA GCAGAGCTCC ATCGCCACCA GGCCGTGTGT
    GCTGATCTCG TGCAGAGGGG ATGCAAACTG GGAGCCTGCG GGCCCCCAAC GGGGCCGGAT
    CCCTGGGAGC GGGCTGAGGT GGTGCAGGGC ATGTGGCAGC GGCTCTGGGC CCGGATGGAT
    GAGCAGGGCA TGAGGCTGCA GGCGGCCCTG CTCGTCCAGC AGTACTTCGT GGACGTGGCG
    GAGGCAGACT CGTGGCTTCA GGAGCAGCGG TCTACACTGG AGAGTGCATC CTTTGGACAG
    GACCAGGTGG CCACCGAGGC CCTACTGCGG CAACACCTGC ATCTGGAACG CTCTGTGCGC
    GCCTTCGGGA CCGAGCTGCG TCGATTAGAT GAGCAGGCAC AGGTGGCCAC AGCCCAGGCA
    GCACTCCTGA GGGAGCCCCC AGAAGGCTGG AGCAGAACTG ATGGAGATGT CACCAGGAGA
    CCTCAGGATG GGACCCAAAT TTTAGCACTG CGGGGCAGCC CTCAGATCAA GACAAGCTTC
    ACCTACAGAG GCACGCAATT CACCCAGCTC CCAGGAGAGG CCCAGCAGCC CCCACACCAG
    ATGGACCCCA ACACCCCAGG AGGCTGCCAG GCCACAGTGG CCCTCAGGCA CATCATGGGA
    CAGGAAGCTC AGGTGGTGTC TGCCCTGAGC TCTCCGAAGA TAGGCCCAAG AAACCCAGGG
    GCCTGGCGTG AGGTTTTCTG CCACTCTGGT CTGTGGCGCA CCCAGAAGAT GACCCTCCCA
    GATGAGCCTG ACCCTGGCTT TGACCCCAAC ACCATCCTCC AGACACAGGA CCGCTTGAAC
    CAGGACTATG AGGGCCTGAG GGCCCAGGCA GAGCGCCGCA GGGCCCAGCT GGAGGAGGCC
    GTGGCCCTGT TTGACTTCTA TGGCTCATGT GGGGAGCTGC AGTCCTGGCT GACCAAACAG
    ACAACGCTGT TGCAAATGCT GCAGCCCGAG GCCAACAACT TGGAAGTTAT GCAGCTCAAG
    TATGAGAACT TCCTCACTGC CCTGGCTGTG GGAAGAGGCC TCTGGACTGA GGTTAACAGC
    TCCGCTGAGC AGCTGAAGCA GAGGTGTCCT GGAGGTTCCC CCCAAATCCA GCTACAGCAG
    GAGGAACTGA GCCAGAGGTG GGAGCAGCTG GAGGCCCTGA AGAAAGAGAA GGAAACGCAG
    CTGGTGTGCA CAACGCACGT GTGCAGTTTT CTGCAGGAGT GTGGATCCAC ACGGGTCCAG
    CTGCAAAACC TGGTACTCCA GCTAGAAAGC TTGGAGCTGG GGAACTCAGA GGATGGCCAC
    CATGTTCTGC GAGTGGGCCA GCAGAAGATG CTGGTGCTGG AGAAATACAT TCGTCACCTG
    CAAAGGGCAG CTAGCAAGGT TGAGGAGATG GCCCCGGCCG AGAGCCCGTC CCTGCAGGCA
    CATGTGGAGA CACTGCAGGG GCTGCTGGAG CGAGTGCGGG AGCAAGTAGC CCAGCGGGCC
    AAGGCCCGGG CCCACCGGAG CTTCCTGCAG GAGAGCGAGC GGCTGCTGCT GTGTGCAGAC
    AGTATCCAGG CCAAGCTCCA CAGCGAGGAG GAGGCGGTGG ATGTGGCCTC AGCCCAGCGG
    CTGCTGGGGA AGCACCGAGA CCTGCTGGAG GAGATCCACC TGCAGCAGGA GAGGCTGCAG
    CAGCTGGAGA CTCAGGGCCA GCCCATGGCA GCCTCGGGCT CCCCAGACTT CCGAGAGGCA
    GCTAGTACCC TGAGGCTCCT GGGCCACCAA GGCCAGGAGT TGAAGGCCGC CTGGGAGCAA
    AGACAGCAGC AACTGCAGGA GGGGCTGGAA TTGCAGAAGT TTGGTCGCGA CGTGGATGGT
    TTCACTGCCT CCTGTGCCAA CCATGAGGCC TTCCTACAGC TGGACGTCCT CCAGGAGGGT
    GTGGTGGAGG CCCAGAGTCT GATGCAGAAG CACCAGGAGT GTGGGCGGCT GCTGGATGCC
    CTTGGCTCTA GAGCAGAGGA TCTACGGGCA CGTGGCAAGA AGCTGGCTCA GAGCCGACAC
    CCCGCTGCAC ACAAGGTCAG AGAACAACTG CAGAGCATCC AGGAGCAGTG GACCAGGGTC
    CAGGAGAGGA GTGAACAGAG AAGGAGGCAG CTGCTGGCCT CCCTCCAGCT CCAGGAATGG
    AAGCAGGACG TGGTGGAGGT GATGCTGTGG ATGGAGGAGA AGGGGCGGGT GTTGGTGGAC
    GAGCCATCTC GAGAGCCCAG CAACATCATG AGGAAACTCA GGCGGCACGA GGCAGCCACG
    TGTGAGCTGG CAGCCACCCG AGGGCACGTG GAGGGCCTGC AGCAGGTCGG GAGAGAGCTG
    CTGAGCAGCA GGCCCCATGC CCAGGAGGAC GTGCAGGCCA GGCTTCAGGG CCTAAGTAGC
    AAATGGGAAG ATTTGAGATG CAAGATGGCA GAGCGTGGGA AGCAGCTCCA GCAGGCTCGG
    CAGCAGGACC AGCTCCTGCG GCTGCTACAG GAAGCCAAGG AGAAGATGGA GCAGCTAGAG
    GGGACTGTGC AGAGGGCAGA GGTGGGACAG GACCTGGGCT CCAGCCAGGG GCTGCAGAAA
    CAGCATTGCC AGCTGGAGGC CGAGAGCCAG GCACTGGCCA GCAAGATGGC TGCCCTTGTC
    CCCGAGGCCC GTCAGGCAAT CAGTTCCCAG GCCATCATGG AGGAGACCCA AAAATACCTC
    CAGAGGTTCA AGGCCCTGCA GGGACATCTG GCCAGCCGGC GACGGCAGCT GCAGGCCTCG
    GTTGAGCTGT ACCACTTCCA CCACCTGAGC AACGCGGAGC TCATGTGGGT GGCAGAGCAC
    ATGCCCACGC CCAGCGCCAG CTCTGCTAAG TGCCCAGATG GTGCCCACCG CCTTCTCCAC
    AAGCACAAGG AACTCCGGGT GCAGGTGAGA GCCCACCAGG GACAGGTGCG ATGGGTGCTG
    GGCTCCGGAC GATACCTCGC AGCCTCCGGG CACCCCCAGG CCCAGCACAT CATGGAGCGG
    TGCCAGGAGC TGGAAGGCCG CTGGGCGGAT CTGGAGCAGG CGTGTGAGGC ACAGGCCCGG
    CACCTGCAGC AGGCTGCTGC CCTCCAGGAG TGTTTCCTGG ACGCATCAGA GCTGGAGGAC
    TGGGTGGAGG AGAAGTGGCC GCTGGTGAGC AGCCAGGACT ATGGTGGGGA TGAGACAGCC
    ACCATCAGGC TCATTAAGAA GCACCAGGCC CTGCAACAGG AACTGGCTCG TTGTTGGAAC
    TCCGTGGAGG AGCTTGACCG GAGAGCCCAA ACCCTCACTG GCCCCGAGGG CCCTGCGCAG
    CTGGGCGTGG TGCAGAAGAG GCTCCGGGAG CGGCTGCAGG CCCTGCAGGA GCTGGCAGCC
    ACACGGGACC GGGAGCTGGA GGGGACCCTG AAGCTGCATG AGTTCATGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC CAGCCAGAAG CAAGTGGCTG GAAGAGGAGA GAGCCTTGGG
    GAAGACTATG AGGACGTCCT GCATCTCTGC ACCAAGTTTT TCAGGTTTCA GCGCACAGTA
    GAGATGGGTG GCCAGCGGGT GGCAAGCTGC CAGCAGCTGG CAGAGAAGCT GCTGGAACGA
    GGGCACAGAG CAGCCCCCAG GGCCCATCAG ATGCAGCAGG ATCTGCAGGC TGCCTGGTCA
    GAGCTATGGG AGCTGACCCA GGCCCGAGGC CGCCTGCTCC GAGACGCTGA GGCCACCCTT
    AAGGTTCACC GAGACATGTT GGAAGCCCTC ACCCAGGTCC AGGAGAAAAC CACCAGCCTC
    CCCTGTGACG TGGCCCAGGA CCTGCGTGGG CTAGAGCCCC AGCTGAGGAG ACATGAGGGG
    CTGGAACGTG AACTCATGGG CATTGACCAG CAGCTGCAGG AGCTGCTGGA GATGGGAGGC
    ACGGTGCAGA AGCTAGGGCC CGGGCCGCAG GCCCATGCAG TACAGCAGAG GCAGCAGGCC
    CTGGTGCAGG CCTGGGAGAC CCTGAAGCTG CGAGCAGAGC AGCGCCGGGC CCAGCTCGAG
    CGGGCATGGC TCCTGGCCCG CTTCCACATG GCGGTGCGGG ACTACACCTC CTGGGTGGCC
    AGCGTGTGGC AGGACCTGCA AGTGGAGGCA GGCTCCCAGG AGCCCGGGAG CGGCCCACTG
    AAGCTCAGCG CCCACCAACA GCTTCGGGCC ATGCTGGAGG CCCGGGAGGA GCTGTACCAG
    CAGGCCGCCC AGCTGGGCCA GCAGGCGCTT CTGGCTGCAG GGACATGCAT CACGGAGGTC
    CGGGACAGGC TGCAAGCCCT GCGGGACAAG CGGGAGCGGG TATTCCAGGC CTGGGAGCAG
    AAGCAGGAGA GGCTGCAGGC CATGGGCCAG GAGCAGCTCT TCCTCAGGAA GTGTGGCCGC
    CTGGACGAGA TCCTGAAGGC CCGGGAGGTC CTCCTGAAAA CCAGTGCCTT GGGGAGCTCA
    GTGGAAGAGG TAGAGCAGTT AATCCGCAAG CATGAGACCT TCCAGAAGGT GCTGGCTGCA
    CAGGAGGAGA AGGAGGCAGC CCTGTGTGAG CAAGTGAAGA TGCTCGGAGG CCCCAGGGCA
    CAGGATCTGC TTTGCACAGT GCTCGAGCGC CGGGCTCGGG TGAAGGAACT GGTGGAGAGT
    CGGCGACACG CCTTGCACAC CTCCCTGATG GTGGCTGCCT TCATCAGGGC CGCCACCCAG
    GCCAAGGACT GGATCCAGGA GCAACTCCAG CAGCTGAAGG AGCCAGTCCC TTCTGGGGAC
    CTGAAAGCTA AGTTGAGACA CCTGCAGAAA CACCAGGCCT TTGAGGCTGA GGTCCAGGCC
    CATGAGGAAA TCATAACCTC TGTTGTCAAG GAGGGCGAGG CTCTCCTAGC ACATAGCCAC
    CCTCTGGTGG GAGAGGTCTC CCAGAGGCTG CGGGAGCTGC AGGAGCACTG GGAGAAGTTG
    AGGCAGGCAG TGGCTCTCCG GGGCGAGGAC CTGGAGGACA AGCGGAACAT CCTGGAGTTC
    CTGCAGAGAG TGGACACTGC AGAGGCCTGG ATCCAGGAGA TGGAGGTGAT GGTGAATGTC
    AGCGACCTGG GCCAGGACCG TGAGCACTGC CAGCAGCTCC ACAGGCAGCT CCGCAAGCTC
    CGAGGAGTCT GGGCCGGGGA CACGGTGGAT GATGCCCGCA TCAGAAGCAT CAGTGACTTC
    TCACCACAGC TCAAGACTCA GGACACAGAG CAACTCAAGA CCATCAGGCA GCGGCAGCAG
    CAGCTCAACA ACAGGCCGGT CCCAGAGCAA GAAGCAGTGG GGCTGGGAGA GGGGGCTGAG
    GGTCCAACCC AGACTCAGCC TTACACCCTC TGCCTCAGGT GGAACAGTTT CCATGGCAAC
    CTGCTCAGAT ACCAGCAGCA GCTCGAAGTG GCCCTGGAAA TACACACGTT GTCCCAAAAG
    CTTGATGACA TCACTCAGCA GGTCAGGAAG AAGGCCGCCC TGGTCCAGGC CCTGGACTGT
    GGGAAAGATC TGGACAATGT GCAGAGGCTG AAGCAGAAAC ACAAGGAGCT GGAGCAGGAA
    ATGGGCCTCC TCCAGGCCCA GGTGGAGCCT CTAGATCGTG AAGCAGGCCG AGTCTGCCAG
    AGAAGCCCTG AGGTGGCCCA CGGCCTCAGC CACCAGCAGC AGGAGATGAT GGACAGCTGG
    CGGCAGCTCC AGAGCAGGAT CCAGAAGTGG AAGGAGTCGC TGGACGCCTT GCAGCAAGCT
    CAGGAGCTGC AGGCAGTGCT TCGGGAACTG CTGGTCTGGG CCCAGAGGCT GCGAGCTCAG
    CTGGACTCCC GGAGAAGCCC CGGCAGCCTC GTGGAGGCCC AGCACATGTT GGAGGAACAC
    CAGGAACTCA AAGCTGAGCT GGATTCCCGA ATGGACAGCA TCAGCCTGGC CCGGAGCACA
    GGGCAGCAAC TGCTTGCTGC GGGGCACCCA TCCACCCCCA ACATTCGCCA GGCCCTGGCT
    GATTTTAATC AGGAGCTGAA CAGCCTGGAA GGGGCCTGGC ATGAGCACCA GCTTCACCTG
    CATCAGGCCC TGGAACTACA GCGGGTTCTG AGCTCTGTGG AGCAGATGGA AAGTTGGCTC
    TCCAGCCTGG AAGCCTGCCC AGCCAGTGAG GGTCTGGGGG ACTGCTTGGC CAACGTGGAG
    ACCCTGCTGT GGAAGCACAG GGTGCGTGAG CAGGACCTGG AGGCTCAGGC GGAGAAGATG
    AGCGCGCTGG AAGCCGCCAG CCGCAGCCTG CTCCAGGGCG GACACCCCGA GGCCCAGGGC
    ACGCTGAGTC GGTGCCAGGC CATGCTTCTG AGGAAAGAGG CACTCCTGGA GCGAGCCAGG
    ACCCGTTGCT GCCAGCTGGA GGAGCTCAGG CAACTGCAGT CTTTCCTGCA GGACTCCTGT
    GAGATGGCCG CCTGGCTGAG GGAGAAGAAC CTGGTGGCCC TGGAAGAGGG CTGGTGGGAC
    CCAGTGGACC TGCAGGCCCA GTTGCAGCAA CAACAGCATC TCCAGGCGGA GCTGGACACC
    CGTGCACACC ACCAACAGAG GCTGCAGATG GAGGGGCAGA GGCTGCTACA GGATGGCCAC
    TTGGCCTCAG AGACCATCCA GGAGCGGCTG CAGGAGCTGA GAAAGCTCTG GGAGGAGCTG
    CAGGCCCAGA GCCAGAGGAG GGCAGCCAAG CTACAGGAGG CCCGTGAGGC CCTGCGTCTG
    CGACGGAGCA TGGAGGAACT GGAGAGCTGG CTGGAGCCTG TGGAGCTCAA GCTGAGAGTC
    CCCATCGGGG GCCAGGACCA GCCTGGGCTG GATGAGCTCC TGGGGGATCA GGGGGAGCTG
    GAGGCAGCAG TGGACAGGCA GGTCGGGCGG GCACAGGCCC TGCTGGGCCA AGCCCAGGCC
    CAGGCCTGTG TCCAGGAAGG TCACTGCCTG GCCCGAGACG TGGAAGAGCA GGCCCAGCGG
    CTGCTTCAGA GGTTCGAGAG CCTGCGGGCG CCCCTGCAGG AGCGCAGGAC AGCCCTGGAG
    GCCCGGAGCC TGCTGCTGCA GTTCTTCAGG GACGCAGAAG AGGAGATGGC CTGGGTGCAG
    GAGAAGCTGC TCCTGGTGGC CGCCCGGGAC TGTGGCCAGA GCCCAAGTGC CCTGCGGCGC
    CTGCAGGAGA AGCACCAGAG CCTGGAGAGT GAGATGAGCA GCCACGAGGC TCTAACCCGG
    GCGGTGGTGG GCACAGGGCG CAAGCTGGTA CAGGCTGGGC ACTTCGCTGC CCGCGATGTG
    GCTGCCCGAG TGCAGCAGCT GGAGGACGCC ATGGGCCGCC TGCGGGCAGA GGCTGCACAG
    AGGCGTCGGC GGCTACAGCA GGCTCAGGAG GCCCAGTACT TCCTGACGGA GCTCCTGGAG
    GCAGAATCCT GGCTGGAAGA ACGGGGCTGT GGCCTGGACA TCGAGGATAT GGGTCAGAGT
    GCCGAGGCCA CGCAGGCTTT CCTACGGCAG CTGGAGGCCA CCAGGAGGGA CCTGGAGGGA
    TTTTCCACGC GCATTGAGAG GCTGCAGCAG ACAGCAGCCC TCCTTGAGAG TGGGCAGAAC
    CCAGAAAGCC CCAAGGTGCT TGCCCAGATG CATGCGGTGA GGGACGCCCA CTCAGGGCTG
    CTGCAGAGAG TGGAGAGTAG GGGGCAAGGC CTGAAGGAGC AGCTGCAGCT CCACCAGCTG
    GAGCGGGAAG CCCTGCTCCT GGATGCCTGG CTGGCCAGCA AAGTGGCCAC TGCCGAGTCC
    CAGGACTACG GGCAGGACCT AGAGGCTGTC AAGCTGCTGG AAGAGAAGTT CGATGCTTTC
    AGGAAGGATG TCCAGAGCCT GGGGCAGGCT AGGGTGCAGG CCCTGAGGGA GCGGGCAGGC
    TCCCTGGAAC GAGCAGCACC CAGGTGCTCT CCTCAGATTC AAGCACAGAG GAGTCGCATT
    GAGGCGTCTT GGGAGAGGCT GGATCAGGCA GTGAAAGCCC GCACACAGAA CCTAGCTGCC
    GCCCGTGAGG TCCGAGGCTT GGAGCAGGCA GCAGCTAAGC TCCAGGCCCG GATGCAAAAG
    AAGGCCACCC TGGTAGCTAG AGATGCCTTC AACCTAAGCC TGTCATCAGT GCAGACTCTG
    CAGCAACAGC ACAGATGTCT GGAGAGGGAA CTGGCAGCGA TGGAAAAGGA GGTGGCACAG
    GTGCAGACGG AGGCCTGCCG CCTGGGCCAG CTCTACCCTG CAGCTCAGGA GGGCCTGGCC
    AAGCAGCTGG CCGGGGTGCA GGAGGCCTGG GCCACCCTGA ACGCAAAGGT CCATGAGCAA
    GATCGGCAGC TGGAGAAGGC TGCCGAGGGC CATGCCTTCC TCGGACGCTG TCGGGAATTG
    CTAGCATGGG CCACGGAGAA GCAGGCCCTG GTGTCCTCGG AGGAGCTGGC TGGGGATATA
    GCTCGGGCTG AGGGCCTCCT GGCACTGCAT GAGAAGCTGG GGAGAGAAAT CAAAGAGTAC
    TGCCTTCAAG CCCAGAACGT ACAGCAGGAA GGGCAGCGGC TGGTGGACAG TGGCCACTGC
    ATGTCCCTGG AGGTAACTGG GTGTCTGCAG GATCTGGACA GGCAGCTGCG GGCACTCAGA
    GAGGCCTGGG CCCTGCGCCG GGAACGCTGT GAGGAGAGCT GGCGTCTACA GAAGCTGCGG
    CAAGAGCTGG ACCAGGCCGA GGCCTGGCTG GCCTGCCGGG AGGGCCTCCT GCTGGACCCC
    AACTGCGGGC ACTCCCTGTC TGATGTGGAG TTGCTGCTCT GCAGACACAA GGACTTAGAG
    AAGCTGCTGG CCTCCCAGGA AGAGAAGTTT GCCCGGCTGC AGCAGGAGGC AGAGGTGGAA
    CAGAAGCAGC AGGTGAAGGG GCTGAAACCC TTGAGAAGCT GGTGGCCCAG AGCACAGCTG
    ACTGAGACAG GGGACCAGCA GCCATCTGTC CCCATTGAGT CTTGCATACG CTTCACCGCC
    GTCTCCCCGG AGTGCACAGC GGGGACCCTG GGGGATGCAA AGGGTGTCCC CACCACGGAG
    GGAATTTTGG AACTTAAGCA GCAGCTCCTG CCTGGGAGAA GGCAGAACAC GGCTTCTACA
    GCCTCCCTCG ACCTCGTAGG AGTGCAGCGT GAGAAGCCGG GAGATCACCA TGATGGAAAA
    CACACTTTTT CCTTAAGGCT AACTGCAGGA GAGACCCTGT CTGCAGCGCC ATCTGCAGGA
    CAGACTGGGA GCTGGCGGGG CACCCTGAGC AGCTGGACAG CCCCCAGTCT GAGCCCAGAG
    CTCAAAGCCA GACCCTCAGG CTTCCTGGCT GAGCGCAATG CTGAGAGGGT ACCCGGCATG
    CCAGCTCCAC TCAGACTCCA CAGCGGTACC CCGCCCGTCT GGCTGCAGGA GACACAAGTG
    TGCACCTATA GCTTCCCGCA GCCCGTGCGG CTCCCACAGA CGGCTGGCCT CGCAGAGCAA
    ACTGCAGAAG GCTCCCACCG ACTGTCCTGC TCAGGCCAGA TCCATGGTCT GGGCCTCGAG
    GCAAAAGCAG AGCTGGCACC AAGCCTCAGG GCTACAAGCG AGGACACCCT GCTATCTGGC
    CGTTCAGGAC CAACTTCTGG AACAGCTGGT TCCTCATCCA GGGATGGGCC CCACCGAGGT
    CAGAGCCTGC TCAAGTCACA CCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328623.1
    CDS49..11535
    Translation

    Target ORF information:

    RefSeq Version XM_015473137.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473137.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    ATGGACTCTG CGTACGAGAT GGGCCACATC CGCAAGCTGC AGGCGCGGCA CATGCAGATG 
    CAGGAGAAGA CCTTCACCAA GTGGATCAAC AATATCTTCC AGCATGGCCG GGTGGGCATC
    GAGATCCAGA ACCTATGCAC AGAGCTGGCC GACGGCACCC ACCTGCTGCG GCTCCTGGAG
    CTCATCTCAG GGGAGGCCCT GCCACCCCCC AACAGGGGCC GCATGCGCGT CCATTTTCTG
    GAGAACAATA GCCGAGCTCT GGCTTTCCTC AGGGCCAAGG TGCCAATACC CCTCATTGGG
    CCAGAGAACA TCGTGGATGG AGACCAGACG CTCATCCTGG GACTCATCTG GGTCATCATT
    CTGCGTTTCC AGATTTCCGA CATCTGCCTG GACAAGGAGG AGTTTGGGGT CAGCGCAGCC
    CTGCTGTCTG CCAGGGAAGC TCTGCTGGTC TGGTGCCAGC GGAAGACTGC CGGCTATGCC
    AACATCAACA TCACGGACTT CTCCCGCAGC TGGAGTGACG GGCTCGGCTT CAGTGCCCTC
    ATCCATGCCC ACAGGCCAGA CCTGCTTGAC TACAGCTCCC TGCGTCCCGA CCGCCCGCTG
    CACAACCTCC GCTGTGCTTT CCACGTAGCT GAGCAGGAGC TGGGCATTGC TCAGCTGCTG
    GATCCAGAGG ATGTGGCAGC CCTACAGCCT GACGAGCGCT CCATAATGAC CTACGTCTCC
    CTCTACTACC ACCACTTCTC CCGCCTGCAC CAGGAGCAGA CCGTCCAGAG GAGACTCACA
    AAGATCCTGC TTCAGCTGCA GGAGTCAGAG GCGCTGCAGA CCCAGTACAA GGAGCTGGTG
    GCCGACCTGC TGTGCTGGAT TGCAGAGAAG CAGGTGCAGC TGGAGGCGCG GGATTTCCCA
    GACAACCTGC CCGCCATGCG CCAGCTACTG GTGGCCTTTG CCTCCTTCCG CAGCCAGGAG
    AAGCCACCGA GACTGCAGCA GCGTGGGGCC ACGGAGGCCC TGCTCTTTCG GCTGCAGACA
    GTCCTCCAAG CCCAGAACCG CAGGCCCTTC CTGCCACCTG AGGGCCTGGG CCCTGCAGAG
    CTGTCCCGGT GCTGGACAAG CCTGGAGCGG GCGGAGGCCT CACGGAGCCG GGCCCTGCAG
    CAGAGGCTCC TACAGCTAGA GCGGTTGGAA ACCCTGGCCC GGCGTTTCCA GCGTAAGGCA
    GCCCTCCGGG AGAGCTTCCT GACAGACACG GAGCAGGTGC TAGACCGGGC CGCAGCCCCG
    CTGGCCAGCC GGGCCATGCT GGAAGCGGCC GCCCAGAGGC TAGGCATGCT GGAAGCCAGT
    ATCCTGCCCC AGGAGGGGCG CTTCCAGGCC CTGGCCGAGA TTGCGGACAT CCTCCATCAG
    GAGCGCTACC ACGGTGGGAC AGAGGTGGCC AGCAGGCAGC TGGAGATCAC CCAGCGCTGG
    GAGCGGCTCC TCCAGCGGCT CCAAGGGCAG AGGAAGCAGA TAGCGGGCAT GCAGGCTGTG
    CTGAGCCTGC TGCAGGAGGT GGAGACTGCC TCCGACCAGC TCAACGAGCT GCAGGTGCTA
    GTCAGCTCCA TGGCCTGTGG GCAGAAGCTG ACAGAGGTAG AGAGGCTGCT GCAGAGGCAT
    GAGCTGCTGG AGGCCCAGAT CTCAGCCCTT GGAGCCCACG TGAGCCATCT GGCCCACCAG
    ACGATGAAGC TGGACTCTTC CATGGGCACT GCTGTGGAGG TGCTGCAGGC CAAGGCCAGG
    GCGCTGGCCC AGCTCTACCA GAGCCTCATG TCTCTTGCTA GGTCCAGGAG GGCCTCACTG
    GAGCAGACGC TGCAGCAGGC AGAGCTCCTG CACAGCTGTG AGGAGGAGGA AGCCTGGTTG
    AGGGAGCATG GACAGCTGGT GGAGGATGCG TCCCCGGGCC CGGATCTCAG CCAGATCGCA
    GCGGCCCTGC AGAAACACAA GGCCCTGGAA GCAGAGCTCC ATCGCCACCA GGCCGTGTGT
    GCTGATCTCG TGCAGAGGGG ATGCAAACTG GGAGCCTGCG GGCCCCCAAC GGGGCCGGAT
    CCCTGGGAGC GGGCTGAGGT GGTGCAGGGC ATGTGGCAGC GGCTCTGGGC CCGGATGGAT
    GAGCAGGGCA TGAGGCTGCA GGCGGCCCTG CTCGTCCAGC AGTACTTCGT GGACGTGGCG
    GAGGCAGACT CGTGGCTTCA GGAGCAGCGG TCTACACTGG AGAGTGCATC CTTTGGACAG
    GACCAGGTGG CCACCGAGGC CCTACTGCGG CAACACCTGC ATCTGGAACG CTCTGTGCGC
    GCCTTCGGGA CCGAGCTGCG TCGATTAGAT GAGCAGGCAC AGGTGGCCAC AGCCCAGGCA
    GCACTCCTGA GGGAGCCCCC AGAAGGCTGG AGCAGAACTG ATGGAGATGT CACCAGGAGA
    CCTCAGGATG GGACCCAAAT TTTAGCACTG CGGGGCAGCC CTCAGATCAA GACAAGCTTC
    ACCTACAGAG GCACGCAATT CACCCAGCTC CCAGGAGAGG CCCAGCAGCC CCCACACCAG
    ATGGACCCCA ACACCCCAGG AGGCTGCCAG GCCACAGTGG CCCTCAGGCA CATCATGGGA
    CAGGAAGCTC AGGTGGTGTC TGCCCTGAGC TCTCCGAAGA TAGGCCCAAG AAACCCAGGG
    GCCTGGCGTG AGGTTTTCTG CCACTCTGGT CTGTGGCGCA CCCAGAAGAT GACCCTCCCA
    GATGAGCCTG ACCCTGGCTT TGACCCCAAC ACCATCCTCC AGACACAGGA CCGCTTGAAC
    CAGGACTATG AGGGCCTGAG GGCCCAGGCA GAGCGCCGCA GGGCCCAGCT GGAGGAGGCC
    GTGGCCCTGT TTGACTTCTA TGGCTCATGT GGGGAGCTGC AGTCCTGGCT GACCAAACAG
    ACAACGCTGT TGCAAATGCT GCAGCCCGAG GCCAACAACT TGGAAGTTAT GCAGCTCAAG
    TATGAGAACT TCCTCACTGC CCTGGCTGTG GGAAGAGGCC TCTGGACTGA GGTTAACAGC
    TCCGCTGAGC AGCTGAAGCA GAGGTGTCCT GGAGGTTCCC CCCAAATCCA GCTACAGCAG
    GAGGAACTGA GCCAGAGGTG GGAGCAGCTG GAGGCCCTGA AGAAAGAGAA GGAAACGCAG
    CTGGTGTGCA CAACGCACGT GTGCAGTTTT CTGCAGGAGT GTGGATCCAC ACGGGTCCAG
    CTGCAAAACC TGGTACTCCA GCTAGAAAGC TTGGAGCTGG GGAACTCAGA GGATGGCCAC
    CATGTTCTGC GAGTGGGCCA GCAGAAGATG CTGGTGCTGG AGAAATACAT TCGTCACCTG
    CAAAGGGCAG CTAGCAAGGT TGAGGAGATG GCCCCGGCCG AGAGCCCGTC CCTGCAGGCA
    CATGTGGAGA CACTGCAGGG GCTGCTGGAG CGAGTGCGGG AGCAAGTAGC CCAGCGGGCC
    AAGGCCCGGG CCCACCGGAG CTTCCTGCAG GAGAGCGAGC GGCTGCTGCT GTGTGCAGAC
    AGTATCCAGG CCAAGCTCCA CAGCGAGGAG GAGGCGGTGG ATGTGGCCTC AGCCCAGCGG
    CTGCTGGGGA AGCACCGAGA CCTGCTGGAG GAGATCCACC TGCAGCAGGA GAGGCTGCAG
    CAGCTGGAGA CTCAGGGCCA GCCCATGGCA GCCTCGGGCT CCCCAGACTT CCGAGAGGCA
    GCTAGTACCC TGAGGCTCCT GGGCCACCAA GGCCAGGAGT TGAAGGCCGC CTGGGAGCAA
    AGACAGCAGC AACTGCAGGA GGGGCTGGAA TTGCAGAAGT TTGGTCGCGA CGTGGATGGT
    TTCACTGCCT CCTGTGCCAA CCATGAGGCC TTCCTACAGC TGGACGTCCT CCAGGAGGGT
    GTGGTGGAGG CCCAGAGTCT GATGCAGAAG CACCAGGAGT GTGGGCGGCT GCTGGATGCC
    CTTGGCTCTA GAGCAGAGGA TCTACGGGCA CGTGGCAAGA AGCTGGCTCA GAGCCGACAC
    CCCGCTGCAC ACAAGGTCAG AGAACAACTG CAGAGCATCC AGGAGCAGTG GACCAGGGTC
    CAGGAGAGGA GTGAACAGAG AAGGAGGCAG CTGCTGGCCT CCCTCCAGCT CCAGGAATGG
    AAGCAGGACG TGGTGGAGGT GATGCTGTGG ATGGAGGAGA AGGGGCGGGT GTTGGTGGAC
    GAGCCATCTC GAGAGCCCAG CAACATCATG AGGAAACTCA GGCGGCACGA GGCAGCCACG
    TGTGAGCTGG CAGCCACCCG AGGGCACGTG GAGGGCCTGC AGCAGGTCGG GAGAGAGCTG
    CTGAGCAGCA GGCCCCATGC CCAGGAGGAC GTGCAGGCCA GGCTTCAGGG CCTAAGTAGC
    AAATGGGAAG ATTTGAGATG CAAGATGGCA GAGCGTGGGA AGCAGCTCCA GCAGGCTCGG
    CAGCAGGACC AGCTCCTGCG GCTGCTACAG GAAGCCAAGG AGAAGATGGA GCAGCTAGAG
    GGGACTGTGC AGAGGGCAGA GGTGGGACAG GACCTGGGCT CCAGCCAGGG GCTGCAGAAA
    CAGCATTGCC AGCTGGAGGC CGAGAGCCAG GCACTGGCCA GCAAGATGGC TGCCCTTGTC
    CCCGAGGCCC GTCAGGCAAT CAGTTCCCAG GCCATCATGG AGGAGACCCA AAAATACCTC
    CAGAGGTTCA AGGCCCTGCA GGGACATCTG GCCAGCCGGC GACGGCAGCT GCAGGCCTCG
    GTTGAGCTGT ACCACTTCCA CCACCTGAGC AACGCGGAGC TCATGTGGGT GGCAGAGCAC
    ATGCCCACGC CCAGCGCCAG CTCTGCTAAG TGCCCAGATG GTGCCCACCG CCTTCTCCAC
    AAGCACAAGG AACTCCGGGT GCAGGTGAGA GCCCACCAGG GACAGGTGCG ATGGGTGCTG
    GGCTCCGGAC GATACCTCGC AGCCTCCGGG CACCCCCAGG CCCAGCACAT CATGGAGCGG
    TGCCAGGAGC TGGAAGGCCG CTGGGCGGAT CTGGAGCAGG CGTGTGAGGC ACAGGCCCGG
    CACCTGCAGC AGGCTGCTGC CCTCCAGGAG TGTTTCCTGG ACGCATCAGA GCTGGAGGAC
    TGGGTGGAGG AGAAGTGGCC GCTGGTGAGC AGCCAGGACT ATGGTGGGGA TGAGACAGCC
    ACCATCAGGC TCATTAAGAA GCACCAGGCC CTGCAACAGG AACTGGCTCG TTGTTGGAAC
    TCCGTGGAGG AGCTTGACCG GAGAGCCCAA ACCCTCACTG GCCCCGAGGG CCCTGCGCAG
    CTGGGCGTGG TGCAGAAGAG GCTCCGGGAG CGGCTGCAGG CCCTGCAGGA GCTGGCAGCC
    ACACGGGACC GGGAGCTGGA GGGGACCCTG AAGCTGCATG AGTTCATGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC CAGCCAGAAG CAAGTGGCTG GAAGAGGAGA GAGCCTTGGG
    GAAGACTATG AGGACGTCCT GCATCTCTGC ACCAAGTTTT TCAGGTTTCA GCGCACAGTA
    GAGATGGGTG GCCAGCGGGT GGCAAGCTGC CAGCAGCTGG CAGAGAAGCT GCTGGAACGA
    GGGCACAGAG CAGCCCCCAG GGCCCATCAG ATGCAGCAGG ATCTGCAGGC TGCCTGGTCA
    GAGCTATGGG AGCTGACCCA GGCCCGAGGC CGCCTGCTCC GAGACGCTGA GGCCACCCTT
    AAGGTTCACC GAGACATGTT GGAAGCCCTC ACCCAGGTCC AGGAGAAAAC CACCAGCCTC
    CCCTGTGACG TGGCCCAGGA CCTGCGTGGG CTAGAGCCCC AGCTGAGGAG ACATGAGGGG
    CTGGAACGTG AACTCATGGG CATTGACCAG CAGCTGCAGG AGCTGCTGGA GATGGGAGGC
    ACGGTGCAGA AGCTAGGGCC CGGGCCGCAG GCCCATGCAG TACAGCAGAG GCAGCAGGCC
    CTGGTGCAGG CCTGGGAGAC CCTGAAGCTG CGAGCAGAGC AGCGCCGGGC CCAGCTCGAG
    CGGGCATGGC TCCTGGCCCG CTTCCACATG GCGGTGCGGG ACTACACCTC CTGGGTGGCC
    AGCGTGTGGC AGGACCTGCA AGTGGAGGCA GGCTCCCAGG AGCCCGGGAG CGGCCCACTG
    AAGCTCAGCG CCCACCAACA GCTTCGGGCC ATGCTGGAGG CCCGGGAGGA GCTGTACCAG
    CAGGCCGCCC AGCTGGGCCA GCAGGCGCTT CTGGCTGCAG GGACATGCAT CACGGAGGTC
    CGGGACAGGC TGCAAGCCCT GCGGGACAAG CGGGAGCGGG TATTCCAGGC CTGGGAGCAG
    AAGCAGGAGA GGCTGCAGGC CATGGGCCAG GAGCAGCTCT TCCTCAGGAA GTGTGGCCGC
    CTGGACGAGA TCCTGAAGGC CCGGGAGGTC CTCCTGAAAA CCAGTGCCTT GGGGAGCTCA
    GTGGAAGAGG TAGAGCAGTT AATCCGCAAG CATGAGACCT TCCAGAAGGT GCTGGCTGCA
    CAGGAGGAGA AGGAGGCAGC CCTGTGTGAG CAAGTGAAGA TGCTCGGAGG CCCCAGGGCA
    CAGGATCTGC TTTGCACAGT GCTCGAGCGC CGGGCTCGGG TGAAGGAACT GGTGGAGAGT
    CGGCGACACG CCTTGCACAC CTCCCTGATG GTGGCTGCCT TCATCAGGGC CGCCACCCAG
    GCCAAGGACT GGATCCAGGA GCAACTCCAG CAGCTGAAGG AGCCAGTCCC TTCTGGGGAC
    CTGAAAGCTA AGTTGAGACA CCTGCAGAAA CACCAGGCCT TTGAGGCTGA GGTCCAGGCC
    CATGAGGAAA TCATAACCTC TGTTGTCAAG GAGGGCGAGG CTCTCCTAGC ACATAGCCAC
    CCTCTGGTGG GAGAGGTCTC CCAGAGGCTG CGGGAGCTGC AGGAGCACTG GGAGAAGTTG
    AGGCAGGCAG TGGCTCTCCG GGGCGAGGAC CTGGAGGACA AGCGGAACAT CCTGGAGTTC
    CTGCAGAGAG TGGACACTGC AGAGGCCTGG ATCCAGGAGA TGGAGGTGAT GGTGAATGTC
    AGCGACCTGG GCCAGGACCG TGAGCACTGC CAGCAGCTCC ACAGGCAGCT CCGCAAGCTC
    CGAGGAGTCT GGGCCGGGGA CACGGTGGAT GATGCCCGCA TCAGAAGCAT CAGTGACTTC
    TCACCACAGC TCAAGACTCA GGACACAGAG CAACTCAAGA CCATCAGGCA GCGGCAGCAG
    CAGCTCAACA ACAGGCCGGT CCCAGAGCAA GAAGCAGTGG GGCTGGGAGA GGGGGCTGAG
    GGTCCAACCC AGACTCAGCC TTACACCCTC TGCCTCAGGT GGAACAGTTT CCATGGCAAC
    CTGCTCAGAT ACCAGCAGCA GCTCGAAGTG GCCCTGGAAA TACACACGTT GTCCCAAAAG
    CTTGATGACA TCACTCAGCA GGTCAGGAAG AAGGCCGCCC TGGTCCAGGC CCTGGACTGT
    GGGAAAGATC TGGACAATGT GCAGAGGCTG AAGCAGAAAC ACAAGGAGCT GGAGCAGGAA
    ATGGGCCTCC TCCAGGCCCA GGTGGAGCCT CTAGATCGTG AAGCAGGCCG AGTCTGCCAG
    AGAAGCCCTG AGGTGGCCCA CGGCCTCAGC CACCAGCAGC AGGAGATGAT GGACAGCTGG
    CGGCAGCTCC AGAGCAGGAT CCAGAAGTGG AAGGAGTCGC TGGACGCCTT GCAGCAAGCT
    CAGGAGCTGC AGGCAGTGCT TCGGGAACTG CTGGTCTGGG CCCAGAGGCT GCGAGCTCAG
    CTGGACTCCC GGAGAAGCCC CGGCAGCCTC GTGGAGGCCC AGCACATGTT GGAGGAACAC
    CAGGAACTCA AAGCTGAGCT GGATTCCCGA ATGGACAGCA TCAGCCTGGC CCGGAGCACA
    GGGCAGCAAC TGCTTGCTGC GGGGCACCCA TCCACCCCCA ACATTCGCCA GGCCCTGGCT
    GATTTTAATC AGGAGCTGAA CAGCCTGGAA GGGGCCTGGC ATGAGCACCA GCTTCACCTG
    CATCAGGCCC TGGAACTACA GCGGGTTCTG AGCTCTGTGG AGCAGATGGA AAGTTGGCTC
    TCCAGCCTGG AAGCCTGCCC AGCCAGTGAG GGTCTGGGGG ACTGCTTGGC CAACGTGGAG
    ACCCTGCTGT GGAAGCACAG GGTGCGTGAG CAGGACCTGG AGGCTCAGGC GGAGAAGATG
    AGCGCGCTGG AAGCCGCCAG CCGCAGCCTG CTCCAGGGCG GACACCCCGA GGCCCAGGGC
    ACGCTGAGTC GGTGCCAGGC CATGCTTCTG AGGAAAGAGG CACTCCTGGA GCGAGCCAGG
    ACCCGTTGCT GCCAGCTGGA GGAGCTCAGG CAACTGCAGT CTTTCCTGCA GGACTCCTGT
    GAGATGGCCG CCTGGCTGAG GGAGAAGAAC CTGGTGGCCC TGGAAGAGGG CTGGTGGGAC
    CCAGTGGACC TGCAGGCCCA GTTGCAGCAA CAACAGCATC TCCAGGCGGA GCTGGACACC
    CGTGCACACC ACCAACAGAG GCTGCAGATG GAGGGGCAGA GGCTGCTACA GGATGGCCAC
    TTGGCCTCAG AGACCATCCA GGAGCGGCTG CAGGAGCTGA GAAAGCTCTG GGAGGAGCTG
    CAGGCCCAGA GCCAGAGGAG GGCAGCCAAG CTACAGGAGG CCCGTGAGGC CCTGCGTCTG
    CGACGGAGCA TGGAGGAACT GGAGAGCTGG CTGGAGCCTG TGGAGCTCAA GCTGAGAGTC
    CCCATCGGGG GCCAGGACCA GCCTGGGCTG GATGAGCTCC TGGGGGATCA GGGGGAGCTG
    GAGGCAGCAG TGGACAGGCA GGTCGGGCGG GCACAGGCCC TGCTGGGCCA AGCCCAGGCC
    CAGGCCTGTG TCCAGGAAGG TCACTGCCTG GCCCGAGACG TGGAAGAGCA GGCCCAGCGG
    CTGCTTCAGA GGTTCGAGAG CCTGCGGGCG CCCCTGCAGG AGCGCAGGAC AGCCCTGGAG
    GCCCGGAGCC TGCTGCTGCA GTTCTTCAGG GACGCAGAAG AGGAGATGGC CTGGGTGCAG
    GAGAAGCTGC TCCTGGTGGC CGCCCGGGAC TGTGGCCAGA GCCCAAGTGC CCTGCGGCGC
    CTGCAGGAGA AGCACCAGAG CCTGGAGAGT GAGATGAGCA GCCACGAGGC TCTAACCCGG
    GCGGTGGTGG GCACAGGGCG CAAGCTGGTA CAGGCTGGGC ACTTCGCTGC CCGCGATGTG
    GCTGCCCGAG TGCAGCAGCT GGAGGACGCC ATGGGCCGCC TGCGGGCAGA GGCTGCACAG
    AGGCGTCGGC GGCTACAGCA GGCTCAGGAG GCCCAGTACT TCCTGACGGA GCTCCTGGAG
    GCAGAATCCT GGCTGGAAGA ACGGGGCTGT GGCCTGGACA TCGAGGATAT GGGTCAGAGT
    GCCGAGGCCA CGCAGGCTTT CCTACGGCAG CTGGAGGCCA CCAGGAGGGA CCTGGAGGGA
    TTTTCCACGC GCATTGAGAG GCTGCAGCAG ACAGCAGCCC TCCTTGAGAG TGGGCAGAAC
    CCAGAAAGCC CCAAGGTGCT TGCCCAGATG CATGCGGTGA GGGACGCCCA CTCAGGGCTG
    CTGCAGAGAG TGGAGAGTAG GGGGCAAGGC CTGAAGGAGC AGCTGCAGCT CCACCAGCTG
    GAGCGGGAAG CCCTGCTCCT GGATGCCTGG CTGGCCAGCA AAGTGGCCAC TGCCGAGTCC
    CAGGACTACG GGCAGGACCT AGAGGCTGTC AAGCTGCTGG AAGAGAAGTT CGATGCTTTC
    AGGAAGGATG TCCAGAGCCT GGGGCAGGCT AGGGTGCAGG CCCTGAGGGA GCGGGCAGGC
    TCCCTGGAAC GAGCAGCACC CAGGTGCTCT CCTCAGATTC AAGCACAGAG GAGTCGCATT
    GAGGCGTCTT GGGAGAGGCT GGATCAGGCA GTGAAAGCCC GCACACAGAA CCTAGCTGCC
    GCCCGTGAGG TCCGAGGCTT GGAGCAGGCA GCAGCTAAGC TCCAGGCCCG GATGCAAAAG
    AAGGCCACCC TGGTAGCTAG AGATGCCTTC AACCTAAGCC TGTCATCAGT GCAGACTCTG
    CAGCAACAGC ACAGATGTCT GGAGAGGGAA CTGGCAGCGA TGGAAAAGGA GGTGGCACAG
    GTGCAGACGG AGGCCTGCCG CCTGGGCCAG CTCTACCCTG CAGCTCAGGA GGGCCTGGCC
    AAGCAGCTGG CCGGGGTGCA GGAGGCCTGG GCCACCCTGA ACGCAAAGGT CCATGAGCAA
    GATCGGCAGC TGGAGAAGGC TGCCGAGGGC CATGCCTTCC TCGGACGCTG TCGGGAATTG
    CTAGCATGGG CCACGGAGAA GCAGGCCCTG GTGTCCTCGG AGGAGCTGGC TGGGGATATA
    GCTCGGGCTG AGGGCCTCCT GGCACTGCAT GAGAAGCTGG GGAGAGAAAT CAAAGAGTAC
    TGCCTTCAAG CCCAGAACGT ACAGCAGGAA GGGCAGCGGC TGGTGGACAG TGGCCACTGC
    ATGTCCCTGG AGGTAACTGG GTGTCTGCAG GATCTGGACA GGCAGCTGCG GGCACTCAGA
    GAGGCCTGGG CCCTGCGCCG GGAACGCTGT GAGGAGAGCT GGCGTCTACA GAAGCTGCGG
    CAAGAGCTGG ACCAGGCCGA GGCCTGGCTG GCCTGCCGGG AGGGCCTCCT GCTGGACCCC
    AACTGCGGGC ACTCCCTGTC TGATGTGGAG TTGCTGCTCT GCAGACACAA GGACTTAGAG
    AAGCTGCTGG CCTCCCAGGA AGAGAAGTTT GCCCGGCTGC AGCAGGAGGC AGAGGTGGAA
    CAGAAGCAGC AGGTGAAGGG GCTGAAACCC TTGAGAAGCT GGTGGCCCAG AGCACAGCTG
    ACTGAGACAG GGGACCAGCA GCCATCTGTC CCCATTGAGT CTTGCATACG CTTCACCGCC
    GTCTCCCCGG AGTGCACAGC GGGGACCCTG GGGGATGCAA AGGGTGTCCC CACCACGGAG
    GGAATTTTGG AACTTAAGCA GCAGCTCCTG CCTGGGAGAA GGCAGAACAC GGCTTCTACA
    GCCTCCCTCG ACCTCGTAGG AGTGCAGCGT GAGAAGCCGG GAGATCACCA TGATGGAAAA
    CACACTTTTT CCTTAAGGCT AACTGCAGGA GAGACCCTGT CTGCAGCGCC ATCTGCAGGA
    CAGACTGGGA GCTGGCGGGG CACCCTGAGC AGCTGGACAG CCCCCAGTCT GAGCCCAGAG
    CTCAAAGCCA GACCCTCAGG CTTCCTGGCT GAGCGCAATG CTGAGAGGGT ACCCGGCATG
    CCAGCTCCAC TCAGACTCCA CAGCGGTACC CCGCCCGTCT GGCTGCAGGA GACACAAGTG
    TGCACCTATA GCTTCCCGCA GCCCGTGCGG CTCCCACAGA CGGCTGGCCT CGCAGAGCAA
    ACTGCAGAAG GCTCCCACCG ACTGTCCTGC TCAGGCCAGA TCCATGGTCT GGGCCTCGAG
    GCAAAAGCAG AGCTGGCACC AAGCCTCAGG GCTACAAGCG AGGACACCCT GCTATCTGGC
    CGTTCAGGAC CAACTTCTGG AACAGCTGGT TCCTCATCCA GGGATGGGCC CCACCGAGGT
    CAGAGCCTGC TCAAGTCACA CCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91679
    Clone ID Related Accession (Same CDS sequence) XM_015465049.1 , XM_015473137.2 , XM_015473137.1
    Accession Version XM_015473137.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    ATGGACTCTG CGTACGAGAT GGGCCACATC CGCAAGCTGC AGGCGCGGCA CATGCAGATG 
    CAGGAGAAGA CCTTCACCAA GTGGATCAAC AATATCTTCC AGCATGGCCG GGTGGGCATC
    GAGATCCAGA ACCTATGCAC AGAGCTGGCC GACGGCACCC ACCTGCTGCG GCTCCTGGAG
    CTCATCTCAG GGGAGGCCCT GCCACCCCCC AACAGGGGCC GCATGCGCGT CCATTTTCTG
    GAGAACAATA GCCGAGCTCT GGCTTTCCTC AGGGCCAAGG TGCCAATACC CCTCATTGGG
    CCAGAGAACA TCGTGGATGG AGACCAGACG CTCATCCTGG GACTCATCTG GGTCATCATT
    CTGCGTTTCC AGATTTCCGA CATCTGCCTG GACAAGGAGG AGTTTGGGGT CAGCGCAGCC
    CTGCTGTCTG CCAGGGAAGC TCTGCTGGTC TGGTGCCAGC GGAAGACTGC CGGCTATGCC
    AACATCAACA TCACGGACTT CTCCCGCAGC TGGAGTGACG GGCTCGGCTT CAGTGCCCTC
    ATCCATGCCC ACAGGCCAGA CCTGCTTGAC TACAGCTCCC TGCGTCCCGA CCGCCCGCTG
    CACAACCTCC GCTGTGCTTT CCACGTAGCT GAGCAGGAGC TGGGCATTGC TCAGCTGCTG
    GATCCAGAGG ATGTGGCAGC CCTACAGCCT GACGAGCGCT CCATAATGAC CTACGTCTCC
    CTCTACTACC ACCACTTCTC CCGCCTGCAC CAGGAGCAGA CCGTCCAGAG GAGACTCACA
    AAGATCCTGC TTCAGCTGCA GGAGTCAGAG GCGCTGCAGA CCCAGTACAA GGAGCTGGTG
    GCCGACCTGC TGTGCTGGAT TGCAGAGAAG CAGGTGCAGC TGGAGGCGCG GGATTTCCCA
    GACAACCTGC CCGCCATGCG CCAGCTACTG GTGGCCTTTG CCTCCTTCCG CAGCCAGGAG
    AAGCCACCGA GACTGCAGCA GCGTGGGGCC ACGGAGGCCC TGCTCTTTCG GCTGCAGACA
    GTCCTCCAAG CCCAGAACCG CAGGCCCTTC CTGCCACCTG AGGGCCTGGG CCCTGCAGAG
    CTGTCCCGGT GCTGGACAAG CCTGGAGCGG GCGGAGGCCT CACGGAGCCG GGCCCTGCAG
    CAGAGGCTCC TACAGCTAGA GCGGTTGGAA ACCCTGGCCC GGCGTTTCCA GCGTAAGGCA
    GCCCTCCGGG AGAGCTTCCT GACAGACACG GAGCAGGTGC TAGACCGGGC CGCAGCCCCG
    CTGGCCAGCC GGGCCATGCT GGAAGCGGCC GCCCAGAGGC TAGGCATGCT GGAAGCCAGT
    ATCCTGCCCC AGGAGGGGCG CTTCCAGGCC CTGGCCGAGA TTGCGGACAT CCTCCATCAG
    GAGCGCTACC ACGGTGGGAC AGAGGTGGCC AGCAGGCAGC TGGAGATCAC CCAGCGCTGG
    GAGCGGCTCC TCCAGCGGCT CCAAGGGCAG AGGAAGCAGA TAGCGGGCAT GCAGGCTGTG
    CTGAGCCTGC TGCAGGAGGT GGAGACTGCC TCCGACCAGC TCAACGAGCT GCAGGTGCTA
    GTCAGCTCCA TGGCCTGTGG GCAGAAGCTG ACAGAGGTAG AGAGGCTGCT GCAGAGGCAT
    GAGCTGCTGG AGGCCCAGAT CTCAGCCCTT GGAGCCCACG TGAGCCATCT GGCCCACCAG
    ACGATGAAGC TGGACTCTTC CATGGGCACT GCTGTGGAGG TGCTGCAGGC CAAGGCCAGG
    GCGCTGGCCC AGCTCTACCA GAGCCTCATG TCTCTTGCTA GGTCCAGGAG GGCCTCACTG
    GAGCAGACGC TGCAGCAGGC AGAGCTCCTG CACAGCTGTG AGGAGGAGGA AGCCTGGTTG
    AGGGAGCATG GACAGCTGGT GGAGGATGCG TCCCCGGGCC CGGATCTCAG CCAGATCGCA
    GCGGCCCTGC AGAAACACAA GGCCCTGGAA GCAGAGCTCC ATCGCCACCA GGCCGTGTGT
    GCTGATCTCG TGCAGAGGGG ATGCAAACTG GGAGCCTGCG GGCCCCCAAC GGGGCCGGAT
    CCCTGGGAGC GGGCTGAGGT GGTGCAGGGC ATGTGGCAGC GGCTCTGGGC CCGGATGGAT
    GAGCAGGGCA TGAGGCTGCA GGCGGCCCTG CTCGTCCAGC AGTACTTCGT GGACGTGGCG
    GAGGCAGACT CGTGGCTTCA GGAGCAGCGG TCTACACTGG AGAGTGCATC CTTTGGACAG
    GACCAGGTGG CCACCGAGGC CCTACTGCGG CAACACCTGC ATCTGGAACG CTCTGTGCGC
    GCCTTCGGGA CCGAGCTGCG TCGATTAGAT GAGCAGGCAC AGGTGGCCAC AGCCCAGGCA
    GCACTCCTGA GGGAGCCCCC AGAAGGCTGG AGCAGAACTG ATGGAGATGT CACCAGGAGA
    CCTCAGGATG GGACCCAAAT TTTAGCACTG CGGGGCAGCC CTCAGATCAA GACAAGCTTC
    ACCTACAGAG GCACGCAATT CACCCAGCTC CCAGGAGAGG CCCAGCAGCC CCCACACCAG
    ATGGACCCCA ACACCCCAGG AGGCTGCCAG GCCACAGTGG CCCTCAGGCA CATCATGGGA
    CAGGAAGCTC AGGTGGTGTC TGCCCTGAGC TCTCCGAAGA TAGGCCCAAG AAACCCAGGG
    GCCTGGCGTG AGGTTTTCTG CCACTCTGGT CTGTGGCGCA CCCAGAAGAT GACCCTCCCA
    GATGAGCCTG ACCCTGGCTT TGACCCCAAC ACCATCCTCC AGACACAGGA CCGCTTGAAC
    CAGGACTATG AGGGCCTGAG GGCCCAGGCA GAGCGCCGCA GGGCCCAGCT GGAGGAGGCC
    GTGGCCCTGT TTGACTTCTA TGGCTCATGT GGGGAGCTGC AGTCCTGGCT GACCAAACAG
    ACAACGCTGT TGCAAATGCT GCAGCCCGAG GCCAACAACT TGGAAGTTAT GCAGCTCAAG
    TATGAGAACT TCCTCACTGC CCTGGCTGTG GGAAGAGGCC TCTGGACTGA GGTTAACAGC
    TCCGCTGAGC AGCTGAAGCA GAGGTGTCCT GGAGGTTCCC CCCAAATCCA GCTACAGCAG
    GAGGAACTGA GCCAGAGGTG GGAGCAGCTG GAGGCCCTGA AGAAAGAGAA GGAAACGCAG
    CTGGTGTGCA CAACGCACGT GTGCAGTTTT CTGCAGGAGT GTGGATCCAC ACGGGTCCAG
    CTGCAAAACC TGGTACTCCA GCTAGAAAGC TTGGAGCTGG GGAACTCAGA GGATGGCCAC
    CATGTTCTGC GAGTGGGCCA GCAGAAGATG CTGGTGCTGG AGAAATACAT TCGTCACCTG
    CAAAGGGCAG CTAGCAAGGT TGAGGAGATG GCCCCGGCCG AGAGCCCGTC CCTGCAGGCA
    CATGTGGAGA CACTGCAGGG GCTGCTGGAG CGAGTGCGGG AGCAAGTAGC CCAGCGGGCC
    AAGGCCCGGG CCCACCGGAG CTTCCTGCAG GAGAGCGAGC GGCTGCTGCT GTGTGCAGAC
    AGTATCCAGG CCAAGCTCCA CAGCGAGGAG GAGGCGGTGG ATGTGGCCTC AGCCCAGCGG
    CTGCTGGGGA AGCACCGAGA CCTGCTGGAG GAGATCCACC TGCAGCAGGA GAGGCTGCAG
    CAGCTGGAGA CTCAGGGCCA GCCCATGGCA GCCTCGGGCT CCCCAGACTT CCGAGAGGCA
    GCTAGTACCC TGAGGCTCCT GGGCCACCAA GGCCAGGAGT TGAAGGCCGC CTGGGAGCAA
    AGACAGCAGC AACTGCAGGA GGGGCTGGAA TTGCAGAAGT TTGGTCGCGA CGTGGATGGT
    TTCACTGCCT CCTGTGCCAA CCATGAGGCC TTCCTACAGC TGGACGTCCT CCAGGAGGGT
    GTGGTGGAGG CCCAGAGTCT GATGCAGAAG CACCAGGAGT GTGGGCGGCT GCTGGATGCC
    CTTGGCTCTA GAGCAGAGGA TCTACGGGCA CGTGGCAAGA AGCTGGCTCA GAGCCGACAC
    CCCGCTGCAC ACAAGGTCAG AGAACAACTG CAGAGCATCC AGGAGCAGTG GACCAGGGTC
    CAGGAGAGGA GTGAACAGAG AAGGAGGCAG CTGCTGGCCT CCCTCCAGCT CCAGGAATGG
    AAGCAGGACG TGGTGGAGGT GATGCTGTGG ATGGAGGAGA AGGGGCGGGT GTTGGTGGAC
    GAGCCATCTC GAGAGCCCAG CAACATCATG AGGAAACTCA GGCGGCACGA GGCAGCCACG
    TGTGAGCTGG CAGCCACCCG AGGGCACGTG GAGGGCCTGC AGCAGGTCGG GAGAGAGCTG
    CTGAGCAGCA GGCCCCATGC CCAGGAGGAC GTGCAGGCCA GGCTTCAGGG CCTAAGTAGC
    AAATGGGAAG ATTTGAGATG CAAGATGGCA GAGCGTGGGA AGCAGCTCCA GCAGGCTCGG
    CAGCAGGACC AGCTCCTGCG GCTGCTACAG GAAGCCAAGG AGAAGATGGA GCAGCTAGAG
    GGGACTGTGC AGAGGGCAGA GGTGGGACAG GACCTGGGCT CCAGCCAGGG GCTGCAGAAA
    CAGCATTGCC AGCTGGAGGC CGAGAGCCAG GCACTGGCCA GCAAGATGGC TGCCCTTGTC
    CCCGAGGCCC GTCAGGCAAT CAGTTCCCAG GCCATCATGG AGGAGACCCA AAAATACCTC
    CAGAGGTTCA AGGCCCTGCA GGGACATCTG GCCAGCCGGC GACGGCAGCT GCAGGCCTCG
    GTTGAGCTGT ACCACTTCCA CCACCTGAGC AACGCGGAGC TCATGTGGGT GGCAGAGCAC
    ATGCCCACGC CCAGCGCCAG CTCTGCTAAG TGCCCAGATG GTGCCCACCG CCTTCTCCAC
    AAGCACAAGG AACTCCGGGT GCAGGTGAGA GCCCACCAGG GACAGGTGCG ATGGGTGCTG
    GGCTCCGGAC GATACCTCGC AGCCTCCGGG CACCCCCAGG CCCAGCACAT CATGGAGCGG
    TGCCAGGAGC TGGAAGGCCG CTGGGCGGAT CTGGAGCAGG CGTGTGAGGC ACAGGCCCGG
    CACCTGCAGC AGGCTGCTGC CCTCCAGGAG TGTTTCCTGG ACGCATCAGA GCTGGAGGAC
    TGGGTGGAGG AGAAGTGGCC GCTGGTGAGC AGCCAGGACT ATGGTGGGGA TGAGACAGCC
    ACCATCAGGC TCATTAAGAA GCACCAGGCC CTGCAACAGG AACTGGCTCG TTGTTGGAAC
    TCCGTGGAGG AGCTTGACCG GAGAGCCCAA ACCCTCACTG GCCCCGAGGG CCCTGCGCAG
    CTGGGCGTGG TGCAGAAGAG GCTCCGGGAG CGGCTGCAGG CCCTGCAGGA GCTGGCAGCC
    ACACGGGACC GGGAGCTGGA GGGGACCCTG AAGCTGCATG AGTTCATGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC CAGCCAGAAG CAAGTGGCTG GAAGAGGAGA GAGCCTTGGG
    GAAGACTATG AGGACGTCCT GCATCTCTGC ACCAAGTTTT TCAGGTTTCA GCGCACAGTA
    GAGATGGGTG GCCAGCGGGT GGCAAGCTGC CAGCAGCTGG CAGAGAAGCT GCTGGAACGA
    GGGCACAGAG CAGCCCCCAG GGCCCATCAG ATGCAGCAGG ATCTGCAGGC TGCCTGGTCA
    GAGCTATGGG AGCTGACCCA GGCCCGAGGC CGCCTGCTCC GAGACGCTGA GGCCACCCTT
    AAGGTTCACC GAGACATGTT GGAAGCCCTC ACCCAGGTCC AGGAGAAAAC CACCAGCCTC
    CCCTGTGACG TGGCCCAGGA CCTGCGTGGG CTAGAGCCCC AGCTGAGGAG ACATGAGGGG
    CTGGAACGTG AACTCATGGG CATTGACCAG CAGCTGCAGG AGCTGCTGGA GATGGGAGGC
    ACGGTGCAGA AGCTAGGGCC CGGGCCGCAG GCCCATGCAG TACAGCAGAG GCAGCAGGCC
    CTGGTGCAGG CCTGGGAGAC CCTGAAGCTG CGAGCAGAGC AGCGCCGGGC CCAGCTCGAG
    CGGGCATGGC TCCTGGCCCG CTTCCACATG GCGGTGCGGG ACTACACCTC CTGGGTGGCC
    AGCGTGTGGC AGGACCTGCA AGTGGAGGCA GGCTCCCAGG AGCCCGGGAG CGGCCCACTG
    AAGCTCAGCG CCCACCAACA GCTTCGGGCC ATGCTGGAGG CCCGGGAGGA GCTGTACCAG
    CAGGCCGCCC AGCTGGGCCA GCAGGCGCTT CTGGCTGCAG GGACATGCAT CACGGAGGTC
    CGGGACAGGC TGCAAGCCCT GCGGGACAAG CGGGAGCGGG TATTCCAGGC CTGGGAGCAG
    AAGCAGGAGA GGCTGCAGGC CATGGGCCAG GAGCAGCTCT TCCTCAGGAA GTGTGGCCGC
    CTGGACGAGA TCCTGAAGGC CCGGGAGGTC CTCCTGAAAA CCAGTGCCTT GGGGAGCTCA
    GTGGAAGAGG TAGAGCAGTT AATCCGCAAG CATGAGACCT TCCAGAAGGT GCTGGCTGCA
    CAGGAGGAGA AGGAGGCAGC CCTGTGTGAG CAAGTGAAGA TGCTCGGAGG CCCCAGGGCA
    CAGGATCTGC TTTGCACAGT GCTCGAGCGC CGGGCTCGGG TGAAGGAACT GGTGGAGAGT
    CGGCGACACG CCTTGCACAC CTCCCTGATG GTGGCTGCCT TCATCAGGGC CGCCACCCAG
    GCCAAGGACT GGATCCAGGA GCAACTCCAG CAGCTGAAGG AGCCAGTCCC TTCTGGGGAC
    CTGAAAGCTA AGTTGAGACA CCTGCAGAAA CACCAGGCCT TTGAGGCTGA GGTCCAGGCC
    CATGAGGAAA TCATAACCTC TGTTGTCAAG GAGGGCGAGG CTCTCCTAGC ACATAGCCAC
    CCTCTGGTGG GAGAGGTCTC CCAGAGGCTG CGGGAGCTGC AGGAGCACTG GGAGAAGTTG
    AGGCAGGCAG TGGCTCTCCG GGGCGAGGAC CTGGAGGACA AGCGGAACAT CCTGGAGTTC
    CTGCAGAGAG TGGACACTGC AGAGGCCTGG ATCCAGGAGA TGGAGGTGAT GGTGAATGTC
    AGCGACCTGG GCCAGGACCG TGAGCACTGC CAGCAGCTCC ACAGGCAGCT CCGCAAGCTC
    CGAGGAGTCT GGGCCGGGGA CACGGTGGAT GATGCCCGCA TCAGAAGCAT CAGTGACTTC
    TCACCACAGC TCAAGACTCA GGACACAGAG CAACTCAAGA CCATCAGGCA GCGGCAGCAG
    CAGCTCAACA ACAGGCCGGT CCCAGAGCAA GAAGCAGTGG GGCTGGGAGA GGGGGCTGAG
    GGTCCAACCC AGACTCAGCC TTACACCCTC TGCCTCAGGT GGAACAGTTT CCATGGCAAC
    CTGCTCAGAT ACCAGCAGCA GCTCGAAGTG GCCCTGGAAA TACACACGTT GTCCCAAAAG
    CTTGATGACA TCACTCAGCA GGTCAGGAAG AAGGCCGCCC TGGTCCAGGC CCTGGACTGT
    GGGAAAGATC TGGACAATGT GCAGAGGCTG AAGCAGAAAC ACAAGGAGCT GGAGCAGGAA
    ATGGGCCTCC TCCAGGCCCA GGTGGAGCCT CTAGATCGTG AAGCAGGCCG AGTCTGCCAG
    AGAAGCCCTG AGGTGGCCCA CGGCCTCAGC CACCAGCAGC AGGAGATGAT GGACAGCTGG
    CGGCAGCTCC AGAGCAGGAT CCAGAAGTGG AAGGAGTCGC TGGACGCCTT GCAGCAAGCT
    CAGGAGCTGC AGGCAGTGCT TCGGGAACTG CTGGTCTGGG CCCAGAGGCT GCGAGCTCAG
    CTGGACTCCC GGAGAAGCCC CGGCAGCCTC GTGGAGGCCC AGCACATGTT GGAGGAACAC
    CAGGAACTCA AAGCTGAGCT GGATTCCCGA ATGGACAGCA TCAGCCTGGC CCGGAGCACA
    GGGCAGCAAC TGCTTGCTGC GGGGCACCCA TCCACCCCCA ACATTCGCCA GGCCCTGGCT
    GATTTTAATC AGGAGCTGAA CAGCCTGGAA GGGGCCTGGC ATGAGCACCA GCTTCACCTG
    CATCAGGCCC TGGAACTACA GCGGGTTCTG AGCTCTGTGG AGCAGATGGA AAGTTGGCTC
    TCCAGCCTGG AAGCCTGCCC AGCCAGTGAG GGTCTGGGGG ACTGCTTGGC CAACGTGGAG
    ACCCTGCTGT GGAAGCACAG GGTGCGTGAG CAGGACCTGG AGGCTCAGGC GGAGAAGATG
    AGCGCGCTGG AAGCCGCCAG CCGCAGCCTG CTCCAGGGCG GACACCCCGA GGCCCAGGGC
    ACGCTGAGTC GGTGCCAGGC CATGCTTCTG AGGAAAGAGG CACTCCTGGA GCGAGCCAGG
    ACCCGTTGCT GCCAGCTGGA GGAGCTCAGG CAACTGCAGT CTTTCCTGCA GGACTCCTGT
    GAGATGGCCG CCTGGCTGAG GGAGAAGAAC CTGGTGGCCC TGGAAGAGGG CTGGTGGGAC
    CCAGTGGACC TGCAGGCCCA GTTGCAGCAA CAACAGCATC TCCAGGCGGA GCTGGACACC
    CGTGCACACC ACCAACAGAG GCTGCAGATG GAGGGGCAGA GGCTGCTACA GGATGGCCAC
    TTGGCCTCAG AGACCATCCA GGAGCGGCTG CAGGAGCTGA GAAAGCTCTG GGAGGAGCTG
    CAGGCCCAGA GCCAGAGGAG GGCAGCCAAG CTACAGGAGG CCCGTGAGGC CCTGCGTCTG
    CGACGGAGCA TGGAGGAACT GGAGAGCTGG CTGGAGCCTG TGGAGCTCAA GCTGAGAGTC
    CCCATCGGGG GCCAGGACCA GCCTGGGCTG GATGAGCTCC TGGGGGATCA GGGGGAGCTG
    GAGGCAGCAG TGGACAGGCA GGTCGGGCGG GCACAGGCCC TGCTGGGCCA AGCCCAGGCC
    CAGGCCTGTG TCCAGGAAGG TCACTGCCTG GCCCGAGACG TGGAAGAGCA GGCCCAGCGG
    CTGCTTCAGA GGTTCGAGAG CCTGCGGGCG CCCCTGCAGG AGCGCAGGAC AGCCCTGGAG
    GCCCGGAGCC TGCTGCTGCA GTTCTTCAGG GACGCAGAAG AGGAGATGGC CTGGGTGCAG
    GAGAAGCTGC TCCTGGTGGC CGCCCGGGAC TGTGGCCAGA GCCCAAGTGC CCTGCGGCGC
    CTGCAGGAGA AGCACCAGAG CCTGGAGAGT GAGATGAGCA GCCACGAGGC TCTAACCCGG
    GCGGTGGTGG GCACAGGGCG CAAGCTGGTA CAGGCTGGGC ACTTCGCTGC CCGCGATGTG
    GCTGCCCGAG TGCAGCAGCT GGAGGACGCC ATGGGCCGCC TGCGGGCAGA GGCTGCACAG
    AGGCGTCGGC GGCTACAGCA GGCTCAGGAG GCCCAGTACT TCCTGACGGA GCTCCTGGAG
    GCAGAATCCT GGCTGGAAGA ACGGGGCTGT GGCCTGGACA TCGAGGATAT GGGTCAGAGT
    GCCGAGGCCA CGCAGGCTTT CCTACGGCAG CTGGAGGCCA CCAGGAGGGA CCTGGAGGGA
    TTTTCCACGC GCATTGAGAG GCTGCAGCAG ACAGCAGCCC TCCTTGAGAG TGGGCAGAAC
    CCAGAAAGCC CCAAGGTGCT TGCCCAGATG CATGCGGTGA GGGACGCCCA CTCAGGGCTG
    CTGCAGAGAG TGGAGAGTAG GGGGCAAGGC CTGAAGGAGC AGCTGCAGCT CCACCAGCTG
    GAGCGGGAAG CCCTGCTCCT GGATGCCTGG CTGGCCAGCA AAGTGGCCAC TGCCGAGTCC
    CAGGACTACG GGCAGGACCT AGAGGCTGTC AAGCTGCTGG AAGAGAAGTT CGATGCTTTC
    AGGAAGGATG TCCAGAGCCT GGGGCAGGCT AGGGTGCAGG CCCTGAGGGA GCGGGCAGGC
    TCCCTGGAAC GAGCAGCACC CAGGTGCTCT CCTCAGATTC AAGCACAGAG GAGTCGCATT
    GAGGCGTCTT GGGAGAGGCT GGATCAGGCA GTGAAAGCCC GCACACAGAA CCTAGCTGCC
    GCCCGTGAGG TCCGAGGCTT GGAGCAGGCA GCAGCTAAGC TCCAGGCCCG GATGCAAAAG
    AAGGCCACCC TGGTAGCTAG AGATGCCTTC AACCTAAGCC TGTCATCAGT GCAGACTCTG
    CAGCAACAGC ACAGATGTCT GGAGAGGGAA CTGGCAGCGA TGGAAAAGGA GGTGGCACAG
    GTGCAGACGG AGGCCTGCCG CCTGGGCCAG CTCTACCCTG CAGCTCAGGA GGGCCTGGCC
    AAGCAGCTGG CCGGGGTGCA GGAGGCCTGG GCCACCCTGA ACGCAAAGGT CCATGAGCAA
    GATCGGCAGC TGGAGAAGGC TGCCGAGGGC CATGCCTTCC TCGGACGCTG TCGGGAATTG
    CTAGCATGGG CCACGGAGAA GCAGGCCCTG GTGTCCTCGG AGGAGCTGGC TGGGGATATA
    GCTCGGGCTG AGGGCCTCCT GGCACTGCAT GAGAAGCTGG GGAGAGAAAT CAAAGAGTAC
    TGCCTTCAAG CCCAGAACGT ACAGCAGGAA GGGCAGCGGC TGGTGGACAG TGGCCACTGC
    ATGTCCCTGG AGGTAACTGG GTGTCTGCAG GATCTGGACA GGCAGCTGCG GGCACTCAGA
    GAGGCCTGGG CCCTGCGCCG GGAACGCTGT GAGGAGAGCT GGCGTCTACA GAAGCTGCGG
    CAAGAGCTGG ACCAGGCCGA GGCCTGGCTG GCCTGCCGGG AGGGCCTCCT GCTGGACCCC
    AACTGCGGGC ACTCCCTGTC TGATGTGGAG TTGCTGCTCT GCAGACACAA GGACTTAGAG
    AAGCTGCTGG CCTCCCAGGA AGAGAAGTTT GCCCGGCTGC AGCAGGAGGC AGAGGTGGAA
    CAGAAGCAGC AGGTGAAGGG GCTGAAACCC TTGAGAAGCT GGTGGCCCAG AGCACAGCTG
    ACTGAGACAG GGGACCAGCA GCCATCTGTC CCCATTGAGT CTTGCATACG CTTCACCGCC
    GTCTCCCCGG AGTGCACAGC GGGGACCCTG GGGGATGCAA AGGGTGTCCC CACCACGGAG
    GGAATTTTGG AACTTAAGCA GCAGCTCCTG CCTGGGAGAA GGCAGAACAC GGCTTCTACA
    GCCTCCCTCG ACCTCGTAGG AGTGCAGCGT GAGAAGCCGG GAGATCACCA TGATGGAAAA
    CACACTTTTT CCTTAAGGCT AACTGCAGGA GAGACCCTGT CTGCAGCGCC ATCTGCAGGA
    CAGACTGGGA GCTGGCGGGG CACCCTGAGC AGCTGGACAG CCCCCAGTCT GAGCCCAGAG
    CTCAAAGCCA GACCCTCAGG CTTCCTGGCT GAGCGCAATG CTGAGAGGGT ACCCGGCATG
    CCAGCTCCAC TCAGACTCCA CAGCGGTACC CCGCCCGTCT GGCTGCAGGA GACACAAGTG
    TGCACCTATA GCTTCCCGCA GCCCGTGCGG CTCCCACAGA CGGCTGGCCT CGCAGAGCAA
    ACTGCAGAAG GCTCCCACCG ACTGTCCTGC TCAGGCCAGA TCCATGGTCT GGGCCTCGAG
    GCAAAAGCAG AGCTGGCACC AAGCCTCAGG GCTACAAGCG AGGACACCCT GCTATCTGGC
    CGTTCAGGAC CAACTTCTGG AACAGCTGGT TCCTCATCCA GGGATGGGCC CCACCGAGGT
    CAGAGCCTGC TCAAGTCACA CCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328623.1
    CDS53..11539
    Translation

    Target ORF information:

    RefSeq Version XM_015473137.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 5 (SPTBN5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    ATGGACTCTG CGTACGAGAT GGGCCACATC CGCAAGCTGC AGGCGCGGCA CATGCAGATG 
    CAGGAGAAGA CCTTCACCAA GTGGATCAAC AATATCTTCC AGCATGGCCG GGTGGGCATC
    GAGATCCAGA ACCTATGCAC AGAGCTGGCC GACGGCACCC ACCTGCTGCG GCTCCTGGAG
    CTCATCTCAG GGGAGGCCCT GCCACCCCCC AACAGGGGCC GCATGCGCGT CCATTTTCTG
    GAGAACAATA GCCGAGCTCT GGCTTTCCTC AGGGCCAAGG TGCCAATACC CCTCATTGGG
    CCAGAGAACA TCGTGGATGG AGACCAGACG CTCATCCTGG GACTCATCTG GGTCATCATT
    CTGCGTTTCC AGATTTCCGA CATCTGCCTG GACAAGGAGG AGTTTGGGGT CAGCGCAGCC
    CTGCTGTCTG CCAGGGAAGC TCTGCTGGTC TGGTGCCAGC GGAAGACTGC CGGCTATGCC
    AACATCAACA TCACGGACTT CTCCCGCAGC TGGAGTGACG GGCTCGGCTT CAGTGCCCTC
    ATCCATGCCC ACAGGCCAGA CCTGCTTGAC TACAGCTCCC TGCGTCCCGA CCGCCCGCTG
    CACAACCTCC GCTGTGCTTT CCACGTAGCT GAGCAGGAGC TGGGCATTGC TCAGCTGCTG
    GATCCAGAGG ATGTGGCAGC CCTACAGCCT GACGAGCGCT CCATAATGAC CTACGTCTCC
    CTCTACTACC ACCACTTCTC CCGCCTGCAC CAGGAGCAGA CCGTCCAGAG GAGACTCACA
    AAGATCCTGC TTCAGCTGCA GGAGTCAGAG GCGCTGCAGA CCCAGTACAA GGAGCTGGTG
    GCCGACCTGC TGTGCTGGAT TGCAGAGAAG CAGGTGCAGC TGGAGGCGCG GGATTTCCCA
    GACAACCTGC CCGCCATGCG CCAGCTACTG GTGGCCTTTG CCTCCTTCCG CAGCCAGGAG
    AAGCCACCGA GACTGCAGCA GCGTGGGGCC ACGGAGGCCC TGCTCTTTCG GCTGCAGACA
    GTCCTCCAAG CCCAGAACCG CAGGCCCTTC CTGCCACCTG AGGGCCTGGG CCCTGCAGAG
    CTGTCCCGGT GCTGGACAAG CCTGGAGCGG GCGGAGGCCT CACGGAGCCG GGCCCTGCAG
    CAGAGGCTCC TACAGCTAGA GCGGTTGGAA ACCCTGGCCC GGCGTTTCCA GCGTAAGGCA
    GCCCTCCGGG AGAGCTTCCT GACAGACACG GAGCAGGTGC TAGACCGGGC CGCAGCCCCG
    CTGGCCAGCC GGGCCATGCT GGAAGCGGCC GCCCAGAGGC TAGGCATGCT GGAAGCCAGT
    ATCCTGCCCC AGGAGGGGCG CTTCCAGGCC CTGGCCGAGA TTGCGGACAT CCTCCATCAG
    GAGCGCTACC ACGGTGGGAC AGAGGTGGCC AGCAGGCAGC TGGAGATCAC CCAGCGCTGG
    GAGCGGCTCC TCCAGCGGCT CCAAGGGCAG AGGAAGCAGA TAGCGGGCAT GCAGGCTGTG
    CTGAGCCTGC TGCAGGAGGT GGAGACTGCC TCCGACCAGC TCAACGAGCT GCAGGTGCTA
    GTCAGCTCCA TGGCCTGTGG GCAGAAGCTG ACAGAGGTAG AGAGGCTGCT GCAGAGGCAT
    GAGCTGCTGG AGGCCCAGAT CTCAGCCCTT GGAGCCCACG TGAGCCATCT GGCCCACCAG
    ACGATGAAGC TGGACTCTTC CATGGGCACT GCTGTGGAGG TGCTGCAGGC CAAGGCCAGG
    GCGCTGGCCC AGCTCTACCA GAGCCTCATG TCTCTTGCTA GGTCCAGGAG GGCCTCACTG
    GAGCAGACGC TGCAGCAGGC AGAGCTCCTG CACAGCTGTG AGGAGGAGGA AGCCTGGTTG
    AGGGAGCATG GACAGCTGGT GGAGGATGCG TCCCCGGGCC CGGATCTCAG CCAGATCGCA
    GCGGCCCTGC AGAAACACAA GGCCCTGGAA GCAGAGCTCC ATCGCCACCA GGCCGTGTGT
    GCTGATCTCG TGCAGAGGGG ATGCAAACTG GGAGCCTGCG GGCCCCCAAC GGGGCCGGAT
    CCCTGGGAGC GGGCTGAGGT GGTGCAGGGC ATGTGGCAGC GGCTCTGGGC CCGGATGGAT
    GAGCAGGGCA TGAGGCTGCA GGCGGCCCTG CTCGTCCAGC AGTACTTCGT GGACGTGGCG
    GAGGCAGACT CGTGGCTTCA GGAGCAGCGG TCTACACTGG AGAGTGCATC CTTTGGACAG
    GACCAGGTGG CCACCGAGGC CCTACTGCGG CAACACCTGC ATCTGGAACG CTCTGTGCGC
    GCCTTCGGGA CCGAGCTGCG TCGATTAGAT GAGCAGGCAC AGGTGGCCAC AGCCCAGGCA
    GCACTCCTGA GGGAGCCCCC AGAAGGCTGG AGCAGAACTG ATGGAGATGT CACCAGGAGA
    CCTCAGGATG GGACCCAAAT TTTAGCACTG CGGGGCAGCC CTCAGATCAA GACAAGCTTC
    ACCTACAGAG GCACGCAATT CACCCAGCTC CCAGGAGAGG CCCAGCAGCC CCCACACCAG
    ATGGACCCCA ACACCCCAGG AGGCTGCCAG GCCACAGTGG CCCTCAGGCA CATCATGGGA
    CAGGAAGCTC AGGTGGTGTC TGCCCTGAGC TCTCCGAAGA TAGGCCCAAG AAACCCAGGG
    GCCTGGCGTG AGGTTTTCTG CCACTCTGGT CTGTGGCGCA CCCAGAAGAT GACCCTCCCA
    GATGAGCCTG ACCCTGGCTT TGACCCCAAC ACCATCCTCC AGACACAGGA CCGCTTGAAC
    CAGGACTATG AGGGCCTGAG GGCCCAGGCA GAGCGCCGCA GGGCCCAGCT GGAGGAGGCC
    GTGGCCCTGT TTGACTTCTA TGGCTCATGT GGGGAGCTGC AGTCCTGGCT GACCAAACAG
    ACAACGCTGT TGCAAATGCT GCAGCCCGAG GCCAACAACT TGGAAGTTAT GCAGCTCAAG
    TATGAGAACT TCCTCACTGC CCTGGCTGTG GGAAGAGGCC TCTGGACTGA GGTTAACAGC
    TCCGCTGAGC AGCTGAAGCA GAGGTGTCCT GGAGGTTCCC CCCAAATCCA GCTACAGCAG
    GAGGAACTGA GCCAGAGGTG GGAGCAGCTG GAGGCCCTGA AGAAAGAGAA GGAAACGCAG
    CTGGTGTGCA CAACGCACGT GTGCAGTTTT CTGCAGGAGT GTGGATCCAC ACGGGTCCAG
    CTGCAAAACC TGGTACTCCA GCTAGAAAGC TTGGAGCTGG GGAACTCAGA GGATGGCCAC
    CATGTTCTGC GAGTGGGCCA GCAGAAGATG CTGGTGCTGG AGAAATACAT TCGTCACCTG
    CAAAGGGCAG CTAGCAAGGT TGAGGAGATG GCCCCGGCCG AGAGCCCGTC CCTGCAGGCA
    CATGTGGAGA CACTGCAGGG GCTGCTGGAG CGAGTGCGGG AGCAAGTAGC CCAGCGGGCC
    AAGGCCCGGG CCCACCGGAG CTTCCTGCAG GAGAGCGAGC GGCTGCTGCT GTGTGCAGAC
    AGTATCCAGG CCAAGCTCCA CAGCGAGGAG GAGGCGGTGG ATGTGGCCTC AGCCCAGCGG
    CTGCTGGGGA AGCACCGAGA CCTGCTGGAG GAGATCCACC TGCAGCAGGA GAGGCTGCAG
    CAGCTGGAGA CTCAGGGCCA GCCCATGGCA GCCTCGGGCT CCCCAGACTT CCGAGAGGCA
    GCTAGTACCC TGAGGCTCCT GGGCCACCAA GGCCAGGAGT TGAAGGCCGC CTGGGAGCAA
    AGACAGCAGC AACTGCAGGA GGGGCTGGAA TTGCAGAAGT TTGGTCGCGA CGTGGATGGT
    TTCACTGCCT CCTGTGCCAA CCATGAGGCC TTCCTACAGC TGGACGTCCT CCAGGAGGGT
    GTGGTGGAGG CCCAGAGTCT GATGCAGAAG CACCAGGAGT GTGGGCGGCT GCTGGATGCC
    CTTGGCTCTA GAGCAGAGGA TCTACGGGCA CGTGGCAAGA AGCTGGCTCA GAGCCGACAC
    CCCGCTGCAC ACAAGGTCAG AGAACAACTG CAGAGCATCC AGGAGCAGTG GACCAGGGTC
    CAGGAGAGGA GTGAACAGAG AAGGAGGCAG CTGCTGGCCT CCCTCCAGCT CCAGGAATGG
    AAGCAGGACG TGGTGGAGGT GATGCTGTGG ATGGAGGAGA AGGGGCGGGT GTTGGTGGAC
    GAGCCATCTC GAGAGCCCAG CAACATCATG AGGAAACTCA GGCGGCACGA GGCAGCCACG
    TGTGAGCTGG CAGCCACCCG AGGGCACGTG GAGGGCCTGC AGCAGGTCGG GAGAGAGCTG
    CTGAGCAGCA GGCCCCATGC CCAGGAGGAC GTGCAGGCCA GGCTTCAGGG CCTAAGTAGC
    AAATGGGAAG ATTTGAGATG CAAGATGGCA GAGCGTGGGA AGCAGCTCCA GCAGGCTCGG
    CAGCAGGACC AGCTCCTGCG GCTGCTACAG GAAGCCAAGG AGAAGATGGA GCAGCTAGAG
    GGGACTGTGC AGAGGGCAGA GGTGGGACAG GACCTGGGCT CCAGCCAGGG GCTGCAGAAA
    CAGCATTGCC AGCTGGAGGC CGAGAGCCAG GCACTGGCCA GCAAGATGGC TGCCCTTGTC
    CCCGAGGCCC GTCAGGCAAT CAGTTCCCAG GCCATCATGG AGGAGACCCA AAAATACCTC
    CAGAGGTTCA AGGCCCTGCA GGGACATCTG GCCAGCCGGC GACGGCAGCT GCAGGCCTCG
    GTTGAGCTGT ACCACTTCCA CCACCTGAGC AACGCGGAGC TCATGTGGGT GGCAGAGCAC
    ATGCCCACGC CCAGCGCCAG CTCTGCTAAG TGCCCAGATG GTGCCCACCG CCTTCTCCAC
    AAGCACAAGG AACTCCGGGT GCAGGTGAGA GCCCACCAGG GACAGGTGCG ATGGGTGCTG
    GGCTCCGGAC GATACCTCGC AGCCTCCGGG CACCCCCAGG CCCAGCACAT CATGGAGCGG
    TGCCAGGAGC TGGAAGGCCG CTGGGCGGAT CTGGAGCAGG CGTGTGAGGC ACAGGCCCGG
    CACCTGCAGC AGGCTGCTGC CCTCCAGGAG TGTTTCCTGG ACGCATCAGA GCTGGAGGAC
    TGGGTGGAGG AGAAGTGGCC GCTGGTGAGC AGCCAGGACT ATGGTGGGGA TGAGACAGCC
    ACCATCAGGC TCATTAAGAA GCACCAGGCC CTGCAACAGG AACTGGCTCG TTGTTGGAAC
    TCCGTGGAGG AGCTTGACCG GAGAGCCCAA ACCCTCACTG GCCCCGAGGG CCCTGCGCAG
    CTGGGCGTGG TGCAGAAGAG GCTCCGGGAG CGGCTGCAGG CCCTGCAGGA GCTGGCAGCC
    ACACGGGACC GGGAGCTGGA GGGGACCCTG AAGCTGCATG AGTTCATGAG GGAGGCTGAG
    GACCTGCAGG GCTGGCTGGC CAGCCAGAAG CAAGTGGCTG GAAGAGGAGA GAGCCTTGGG
    GAAGACTATG AGGACGTCCT GCATCTCTGC ACCAAGTTTT TCAGGTTTCA GCGCACAGTA
    GAGATGGGTG GCCAGCGGGT GGCAAGCTGC CAGCAGCTGG CAGAGAAGCT GCTGGAACGA
    GGGCACAGAG CAGCCCCCAG GGCCCATCAG ATGCAGCAGG ATCTGCAGGC TGCCTGGTCA
    GAGCTATGGG AGCTGACCCA GGCCCGAGGC CGCCTGCTCC GAGACGCTGA GGCCACCCTT
    AAGGTTCACC GAGACATGTT GGAAGCCCTC ACCCAGGTCC AGGAGAAAAC CACCAGCCTC
    CCCTGTGACG TGGCCCAGGA CCTGCGTGGG CTAGAGCCCC AGCTGAGGAG ACATGAGGGG
    CTGGAACGTG AACTCATGGG CATTGACCAG CAGCTGCAGG AGCTGCTGGA GATGGGAGGC
    ACGGTGCAGA AGCTAGGGCC CGGGCCGCAG GCCCATGCAG TACAGCAGAG GCAGCAGGCC
    CTGGTGCAGG CCTGGGAGAC CCTGAAGCTG CGAGCAGAGC AGCGCCGGGC CCAGCTCGAG
    CGGGCATGGC TCCTGGCCCG CTTCCACATG GCGGTGCGGG ACTACACCTC CTGGGTGGCC
    AGCGTGTGGC AGGACCTGCA AGTGGAGGCA GGCTCCCAGG AGCCCGGGAG CGGCCCACTG
    AAGCTCAGCG CCCACCAACA GCTTCGGGCC ATGCTGGAGG CCCGGGAGGA GCTGTACCAG
    CAGGCCGCCC AGCTGGGCCA GCAGGCGCTT CTGGCTGCAG GGACATGCAT CACGGAGGTC
    CGGGACAGGC TGCAAGCCCT GCGGGACAAG CGGGAGCGGG TATTCCAGGC CTGGGAGCAG
    AAGCAGGAGA GGCTGCAGGC CATGGGCCAG GAGCAGCTCT TCCTCAGGAA GTGTGGCCGC
    CTGGACGAGA TCCTGAAGGC CCGGGAGGTC CTCCTGAAAA CCAGTGCCTT GGGGAGCTCA
    GTGGAAGAGG TAGAGCAGTT AATCCGCAAG CATGAGACCT TCCAGAAGGT GCTGGCTGCA
    CAGGAGGAGA AGGAGGCAGC CCTGTGTGAG CAAGTGAAGA TGCTCGGAGG CCCCAGGGCA
    CAGGATCTGC TTTGCACAGT GCTCGAGCGC CGGGCTCGGG TGAAGGAACT GGTGGAGAGT
    CGGCGACACG CCTTGCACAC CTCCCTGATG GTGGCTGCCT TCATCAGGGC CGCCACCCAG
    GCCAAGGACT GGATCCAGGA GCAACTCCAG CAGCTGAAGG AGCCAGTCCC TTCTGGGGAC
    CTGAAAGCTA AGTTGAGACA CCTGCAGAAA CACCAGGCCT TTGAGGCTGA GGTCCAGGCC
    CATGAGGAAA TCATAACCTC TGTTGTCAAG GAGGGCGAGG CTCTCCTAGC ACATAGCCAC
    CCTCTGGTGG GAGAGGTCTC CCAGAGGCTG CGGGAGCTGC AGGAGCACTG GGAGAAGTTG
    AGGCAGGCAG TGGCTCTCCG GGGCGAGGAC CTGGAGGACA AGCGGAACAT CCTGGAGTTC
    CTGCAGAGAG TGGACACTGC AGAGGCCTGG ATCCAGGAGA TGGAGGTGAT GGTGAATGTC
    AGCGACCTGG GCCAGGACCG TGAGCACTGC CAGCAGCTCC ACAGGCAGCT CCGCAAGCTC
    CGAGGAGTCT GGGCCGGGGA CACGGTGGAT GATGCCCGCA TCAGAAGCAT CAGTGACTTC
    TCACCACAGC TCAAGACTCA GGACACAGAG CAACTCAAGA CCATCAGGCA GCGGCAGCAG
    CAGCTCAACA ACAGGCCGGT CCCAGAGCAA GAAGCAGTGG GGCTGGGAGA GGGGGCTGAG
    GGTCCAACCC AGACTCAGCC TTACACCCTC TGCCTCAGGT GGAACAGTTT CCATGGCAAC
    CTGCTCAGAT ACCAGCAGCA GCTCGAAGTG GCCCTGGAAA TACACACGTT GTCCCAAAAG
    CTTGATGACA TCACTCAGCA GGTCAGGAAG AAGGCCGCCC TGGTCCAGGC CCTGGACTGT
    GGGAAAGATC TGGACAATGT GCAGAGGCTG AAGCAGAAAC ACAAGGAGCT GGAGCAGGAA
    ATGGGCCTCC TCCAGGCCCA GGTGGAGCCT CTAGATCGTG AAGCAGGCCG AGTCTGCCAG
    AGAAGCCCTG AGGTGGCCCA CGGCCTCAGC CACCAGCAGC AGGAGATGAT GGACAGCTGG
    CGGCAGCTCC AGAGCAGGAT CCAGAAGTGG AAGGAGTCGC TGGACGCCTT GCAGCAAGCT
    CAGGAGCTGC AGGCAGTGCT TCGGGAACTG CTGGTCTGGG CCCAGAGGCT GCGAGCTCAG
    CTGGACTCCC GGAGAAGCCC CGGCAGCCTC GTGGAGGCCC AGCACATGTT GGAGGAACAC
    CAGGAACTCA AAGCTGAGCT GGATTCCCGA ATGGACAGCA TCAGCCTGGC CCGGAGCACA
    GGGCAGCAAC TGCTTGCTGC GGGGCACCCA TCCACCCCCA ACATTCGCCA GGCCCTGGCT
    GATTTTAATC AGGAGCTGAA CAGCCTGGAA GGGGCCTGGC ATGAGCACCA GCTTCACCTG
    CATCAGGCCC TGGAACTACA GCGGGTTCTG AGCTCTGTGG AGCAGATGGA AAGTTGGCTC
    TCCAGCCTGG AAGCCTGCCC AGCCAGTGAG GGTCTGGGGG ACTGCTTGGC CAACGTGGAG
    ACCCTGCTGT GGAAGCACAG GGTGCGTGAG CAGGACCTGG AGGCTCAGGC GGAGAAGATG
    AGCGCGCTGG AAGCCGCCAG CCGCAGCCTG CTCCAGGGCG GACACCCCGA GGCCCAGGGC
    ACGCTGAGTC GGTGCCAGGC CATGCTTCTG AGGAAAGAGG CACTCCTGGA GCGAGCCAGG
    ACCCGTTGCT GCCAGCTGGA GGAGCTCAGG CAACTGCAGT CTTTCCTGCA GGACTCCTGT
    GAGATGGCCG CCTGGCTGAG GGAGAAGAAC CTGGTGGCCC TGGAAGAGGG CTGGTGGGAC
    CCAGTGGACC TGCAGGCCCA GTTGCAGCAA CAACAGCATC TCCAGGCGGA GCTGGACACC
    CGTGCACACC ACCAACAGAG GCTGCAGATG GAGGGGCAGA GGCTGCTACA GGATGGCCAC
    TTGGCCTCAG AGACCATCCA GGAGCGGCTG CAGGAGCTGA GAAAGCTCTG GGAGGAGCTG
    CAGGCCCAGA GCCAGAGGAG GGCAGCCAAG CTACAGGAGG CCCGTGAGGC CCTGCGTCTG
    CGACGGAGCA TGGAGGAACT GGAGAGCTGG CTGGAGCCTG TGGAGCTCAA GCTGAGAGTC
    CCCATCGGGG GCCAGGACCA GCCTGGGCTG GATGAGCTCC TGGGGGATCA GGGGGAGCTG
    GAGGCAGCAG TGGACAGGCA GGTCGGGCGG GCACAGGCCC TGCTGGGCCA AGCCCAGGCC
    CAGGCCTGTG TCCAGGAAGG TCACTGCCTG GCCCGAGACG TGGAAGAGCA GGCCCAGCGG
    CTGCTTCAGA GGTTCGAGAG CCTGCGGGCG CCCCTGCAGG AGCGCAGGAC AGCCCTGGAG
    GCCCGGAGCC TGCTGCTGCA GTTCTTCAGG GACGCAGAAG AGGAGATGGC CTGGGTGCAG
    GAGAAGCTGC TCCTGGTGGC CGCCCGGGAC TGTGGCCAGA GCCCAAGTGC CCTGCGGCGC
    CTGCAGGAGA AGCACCAGAG CCTGGAGAGT GAGATGAGCA GCCACGAGGC TCTAACCCGG
    GCGGTGGTGG GCACAGGGCG CAAGCTGGTA CAGGCTGGGC ACTTCGCTGC CCGCGATGTG
    GCTGCCCGAG TGCAGCAGCT GGAGGACGCC ATGGGCCGCC TGCGGGCAGA GGCTGCACAG
    AGGCGTCGGC GGCTACAGCA GGCTCAGGAG GCCCAGTACT TCCTGACGGA GCTCCTGGAG
    GCAGAATCCT GGCTGGAAGA ACGGGGCTGT GGCCTGGACA TCGAGGATAT GGGTCAGAGT
    GCCGAGGCCA CGCAGGCTTT CCTACGGCAG CTGGAGGCCA CCAGGAGGGA CCTGGAGGGA
    TTTTCCACGC GCATTGAGAG GCTGCAGCAG ACAGCAGCCC TCCTTGAGAG TGGGCAGAAC
    CCAGAAAGCC CCAAGGTGCT TGCCCAGATG CATGCGGTGA GGGACGCCCA CTCAGGGCTG
    CTGCAGAGAG TGGAGAGTAG GGGGCAAGGC CTGAAGGAGC AGCTGCAGCT CCACCAGCTG
    GAGCGGGAAG CCCTGCTCCT GGATGCCTGG CTGGCCAGCA AAGTGGCCAC TGCCGAGTCC
    CAGGACTACG GGCAGGACCT AGAGGCTGTC AAGCTGCTGG AAGAGAAGTT CGATGCTTTC
    AGGAAGGATG TCCAGAGCCT GGGGCAGGCT AGGGTGCAGG CCCTGAGGGA GCGGGCAGGC
    TCCCTGGAAC GAGCAGCACC CAGGTGCTCT CCTCAGATTC AAGCACAGAG GAGTCGCATT
    GAGGCGTCTT GGGAGAGGCT GGATCAGGCA GTGAAAGCCC GCACACAGAA CCTAGCTGCC
    GCCCGTGAGG TCCGAGGCTT GGAGCAGGCA GCAGCTAAGC TCCAGGCCCG GATGCAAAAG
    AAGGCCACCC TGGTAGCTAG AGATGCCTTC AACCTAAGCC TGTCATCAGT GCAGACTCTG
    CAGCAACAGC ACAGATGTCT GGAGAGGGAA CTGGCAGCGA TGGAAAAGGA GGTGGCACAG
    GTGCAGACGG AGGCCTGCCG CCTGGGCCAG CTCTACCCTG CAGCTCAGGA GGGCCTGGCC
    AAGCAGCTGG CCGGGGTGCA GGAGGCCTGG GCCACCCTGA ACGCAAAGGT CCATGAGCAA
    GATCGGCAGC TGGAGAAGGC TGCCGAGGGC CATGCCTTCC TCGGACGCTG TCGGGAATTG
    CTAGCATGGG CCACGGAGAA GCAGGCCCTG GTGTCCTCGG AGGAGCTGGC TGGGGATATA
    GCTCGGGCTG AGGGCCTCCT GGCACTGCAT GAGAAGCTGG GGAGAGAAAT CAAAGAGTAC
    TGCCTTCAAG CCCAGAACGT ACAGCAGGAA GGGCAGCGGC TGGTGGACAG TGGCCACTGC
    ATGTCCCTGG AGGTAACTGG GTGTCTGCAG GATCTGGACA GGCAGCTGCG GGCACTCAGA
    GAGGCCTGGG CCCTGCGCCG GGAACGCTGT GAGGAGAGCT GGCGTCTACA GAAGCTGCGG
    CAAGAGCTGG ACCAGGCCGA GGCCTGGCTG GCCTGCCGGG AGGGCCTCCT GCTGGACCCC
    AACTGCGGGC ACTCCCTGTC TGATGTGGAG TTGCTGCTCT GCAGACACAA GGACTTAGAG
    AAGCTGCTGG CCTCCCAGGA AGAGAAGTTT GCCCGGCTGC AGCAGGAGGC AGAGGTGGAA
    CAGAAGCAGC AGGTGAAGGG GCTGAAACCC TTGAGAAGCT GGTGGCCCAG AGCACAGCTG
    ACTGAGACAG GGGACCAGCA GCCATCTGTC CCCATTGAGT CTTGCATACG CTTCACCGCC
    GTCTCCCCGG AGTGCACAGC GGGGACCCTG GGGGATGCAA AGGGTGTCCC CACCACGGAG
    GGAATTTTGG AACTTAAGCA GCAGCTCCTG CCTGGGAGAA GGCAGAACAC GGCTTCTACA
    GCCTCCCTCG ACCTCGTAGG AGTGCAGCGT GAGAAGCCGG GAGATCACCA TGATGGAAAA
    CACACTTTTT CCTTAAGGCT AACTGCAGGA GAGACCCTGT CTGCAGCGCC ATCTGCAGGA
    CAGACTGGGA GCTGGCGGGG CACCCTGAGC AGCTGGACAG CCCCCAGTCT GAGCCCAGAG
    CTCAAAGCCA GACCCTCAGG CTTCCTGGCT GAGCGCAATG CTGAGAGGGT ACCCGGCATG
    CCAGCTCCAC TCAGACTCCA CAGCGGTACC CCGCCCGTCT GGCTGCAGGA GACACAAGTG
    TGCACCTATA GCTTCCCGCA GCCCGTGCGG CTCCCACAGA CGGCTGGCCT CGCAGAGCAA
    ACTGCAGAAG GCTCCCACCG ACTGTCCTGC TCAGGCCAGA TCCATGGTCT GGGCCTCGAG
    GCAAAAGCAG AGCTGGCACC AAGCCTCAGG GCTACAAGCG AGGACACCCT GCTATCTGGC
    CGTTCAGGAC CAACTTCTGG AACAGCTGGT TCCTCATCCA GGGATGGGCC CCACCGAGGT
    CAGAGCCTGC TCAAGTCACA CCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.