BARD1 cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BARD1
Entrez Gene ID	530961
Full Name	BRCA1 associated RING domain 1
General protein information	Preferred Names BRCA1 associated RING domain 1 Names BRCA1-associated RING domain protein 1
Gene Type	protein-coding
Organism	Bos taurus(Cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192701.2	NP_001179630.2	BRCA1-associated RING domain protein 1
XM_005202787.3	XP_005202844.1	BRCA1-associated RING domain protein 1 isoform X1
XM_005202787.2	XP_005202844.1	BRCA1-associated RING domain protein 1 isoform X1
NM_001192701.1	NP_001179630.1	BRCA1-associated RING domain protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BARD1	NP_001179630.1
Xenopus tropicalis (tropical clawed frog)	bard1	XP_002932040.2
Homo sapiens (human)	BARD1	NP_000456.2
Pan troglodytes (chimpanzee)	BARD1	XP_526019.2
Rattus norvegicus (Norway rat)	Bard1	NP_072144.1
Gallus gallus (chicken)	BARD1	NP_001026417.1
	BARD1	XP_001084740.1
Canis lupus familiaris (dog)	BARD1	XP_005640797.1
Mus musculus (house mouse)	Bard1	NP_031551.1
Danio rerio (zebrafish)	LOC100147913	XP_001921099.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BARD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BARD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36072	NM_001192701.1 Latest version!	Bos taurus BRCA1 associated RING domain 1 (BARD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa16680	XM_005202787.3 Latest version!	Bos taurus BRCA1 associated RING domain 1 (BARD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa216288	NM_001192701.2 Latest version!	Bos taurus BRCA1 associated RING domain 1 (BARD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa16680	XM_005202787.2	Bos taurus BRCA1 associated RING domain 1 (BARD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BARD1 ( NM_001192701.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001179630.1 Bos taurus BRCA1 associated RING domain 1 (BARD1), mRNA.

Price & Availability↑

CloneID	OBa36072
Clone ID Related Accession (Same CDS sequence)	NM_001192701.1
Accession Version	NM_001192701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	BRCA1-associated RING domain protein 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC02130299.1, AAFC03040682.1, AAFC03040683.1, AAFC03120437.1 and AAFC03039326.1. On Jul 17, 2010 this sequence version replaced XM_609443.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGGAAGAGAT CTCTAGTCTC TCCCATTCTG TTGTAAAAAC TGGTCAGCGT 
CGGGATGGGC CTCTTGTACT TATAGGCAGT GGACTTTCTT CCGAACAACA GAAAATGCTC 
AGTGAGCTTG CAACGATTCT TAAGGCTAAA AAATGTGCCG AGTTTGACGG TACAGTAACT 
CATGTCATTG TTCCTGGTGA TACAGTTCAA AGCACCCTGA AATGTATGCT TGGAATTCTC 
AGTGGATGCT GGATCCTAAA ATTTGAATGG GTGAAAGCAT GTCTACAAAG CAAAGAATGT 
GAACAGGAAG AAAAGTATGA AATTCCTGAA GGACCACAGA AAAGCAGGCT CAACAGAGAA 
CAGCTGTTGC CAAAGCTGTT TGATGGATGT TACTTCTATT TGGGGGGAAC CTTCAAACAC 
CATCCAAAGG ACAACCTTAT TAAGCTTGTC GCTGCCGGTG GGGGCCAGAT CCTCATTAGA 
AAGCCCAAGC CAGACAGTGA CGTGACTCAA AGCATCAATA CAGTCGCGTA CCATGCCAAA 
CCTGATTCTG ATCAGCGCTT CTGCACACAG TATATCATCT ATGAGGACTT GTCTAATCAT 
CGCCCAGAAA GGGTTCGGCA GGGCAAAGTC TGGATGGCTC CTTCCAGCTG GTTTATAGAT 
TGTGTGATGT CCTTTGAGTT GCTCCCTCTT GACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179630.1
CDS	112..2430
Translation	MQGNRQPRVRSGNQPHPAPAMKPAGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGSECPVCYTPAWIQDVKINRQLDSMIQLCSKLQNLLHDTDLSDLKEKTSRKSLFNDAQSKKNSIKMWFSPRSKKVRYTVSKLSVQTQPSVKNDENAQQTSMYEFVSPSPPVEVSEGPKKPSTRSRKKQKKKTLAEVNRKWNLEAEKEDGDLDCKGESKEKLVSFCSQPSVIANLQINGEIDLLASGSVTESECLDEVFLPLAEHIESPEIESKNEVVTPEKTVSANYLPSKKSLPSGRNGKRGRHNRISSPVSKRCRSSSQSTGGNSVKQTVLLENMPLLGCSSPPSNKLKVGDTLSRNSRTILDESISLSPGTPPSTLNSPSYRRMMCSPSATKLWPISHTAVKRNHRGETLLHIASIKGDIPSVEYLLQSGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAVKNGHVDIVKLLLTYGASREAVNIFGLRPVDYADSENMKSLLLLPEKEEISSLSHSVVKTGQRRDGPLVLIGSGLSSEQQKMLSELATILKAKKCAEFDGTVTHVIVPGDTVQSTLKCMLGILSGCWILKFEWVKACLQSKECEQEEKYEIPEGPQKSRLNREQLLPKLFDGCYFYLGGTFKHHPKDNLIKLVAAGGGQILIRKPKPDSDVTQSINTVAYHAKPDSDQRFCTQYIIYEDLSNHRPERVRQGKVWMAPSSWFIDCVMSFELLPLDN

Target ORF information:

RefSeq Version	NM_001192701.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus BRCA1 associated RING domain 1 (BARD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192701.1

ORF Insert Sequence:

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGGAAGAGAT CTCTAGTCTC TCCCATTCTG TTGTAAAAAC TGGTCAGCGT 
CGGGATGGGC CTCTTGTACT TATAGGCAGT GGACTTTCTT CCGAACAACA GAAAATGCTC 
AGTGAGCTTG CAACGATTCT TAAGGCTAAA AAATGTGCCG AGTTTGACGG TACAGTAACT 
CATGTCATTG TTCCTGGTGA TACAGTTCAA AGCACCCTGA AATGTATGCT TGGAATTCTC 
AGTGGATGCT GGATCCTAAA ATTTGAATGG GTGAAAGCAT GTCTACAAAG CAAAGAATGT 
GAACAGGAAG AAAAGTATGA AATTCCTGAA GGACCACAGA AAAGCAGGCT CAACAGAGAA 
CAGCTGTTGC CAAAGCTGTT TGATGGATGT TACTTCTATT TGGGGGGAAC CTTCAAACAC 
CATCCAAAGG ACAACCTTAT TAAGCTTGTC GCTGCCGGTG GGGGCCAGAT CCTCATTAGA 
AAGCCCAAGC CAGACAGTGA CGTGACTCAA AGCATCAATA CAGTCGCGTA CCATGCCAAA 
CCTGATTCTG ATCAGCGCTT CTGCACACAG TATATCATCT ATGAGGACTT GTCTAATCAT 
CGCCCAGAAA GGGTTCGGCA GGGCAAAGTC TGGATGGCTC CTTCCAGCTG GTTTATAGAT 
TGTGTGATGT CCTTTGAGTT GCTCCCTCTT GACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BARD1 ( XM_005202787.3 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005202844.1 Bos taurus BRCA1 associated RING domain 1 (BARD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa16680
Clone ID Related Accession (Same CDS sequence)	XM_005202787.3 , XM_005202787.2
Accession Version	XM_005202787.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	BRCA1-associated RING domain protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202787.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGAAGGAATC CTCATCAACC AGCCATTGCT CAGTAGTAAA CACTGGTCAG 
CGTCGGGATG GGCCTCTTGT ACTTATAGGC AGTGGACTTT CTTCCGAACA ACAGAAAATG 
CTCAGTGAGC TTGCAACGAT TCTTAAGGCT AAAAAATGTG CCGAGTTTGA CGGTACAGTA 
ACTCATGTCA TTGTTCCTGG TGATACAGTT CAAAGCACCC TGAAATGTAT GCTTGGAATT 
CTCAGTGGAT GCTGGATCCT AAAATTTGAA TGGGTGAAAG CATGTCTACA AAGCAAAGAA 
TGTGAACAGG AAGAAAAGTA TGAAATTCCT GAAGGACCAC AGAAAAGCAG GCTCAACAGA 
GAACAGCTGT TGCCAAAGCT GTTTGATGGA TGTTACTTCT ATTTGGGGGG AACCTTCAAA 
CACCATCCAA AGGACAACCT TATTAAGCTT GTCGCTGCCG GTGGGGGCCA GATCCTCATT 
AGAAAGCCCA AGCCAGACAG TGACGTGACT CAAAGCATCA ATACAGTCGC GTACCATGCC 
AAACCTGATT CTGATCAGCG CTTCTGCACA CAGTATATCA TCTATGAGGA CTTGTCTAAT 
CATCGCCCAG AAAGGGTTCG GCAGGGCAAA GTCTGGATGG CTCCTTCCAG CTGGTTTATA 
GATTGTGTGA TGTCCTTTGA GTTGCTCCCT CTTGACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202844.1
CDS	143..2464
Translation	MQGNRQPRVRSGNQPHPAPAMKPAGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGSECPVCYTPAWIQDVKINRQLDSMIQLCSKLQNLLHDTDLSDLKEKTSRKSLFNDAQSKKNSIKMWFSPRSKKVRYTVSKLSVQTQPSVKNDENAQQTSMYEFVSPSPPVEVSEGPKKPSTRSRKKQKKKTLAEVNRKWNLEAEKEDGDLDCKGESKEKLVSFCSQPSVIANLQINGEIDLLASGSVTESECLDEVFLPLAEHIESPEIESKNEVVTPEKTVSANYLPSKKSLPSGRNGKRGRHNRISSPVSKRCRSSSQSTGGNSVKQTVLLENMPLLGCSSPPSNKLKVGDTLSRNSRTILDESISLSPGTPPSTLNSPSYRRMMCSPSATKLWPISHTAVKRNHRGETLLHIASIKGDIPSVEYLLQSGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAVKNGHVDIVKLLLTYGASREAVNIFGLRPVDYADSENMKSLLLLPEKKESSSTSHCSVVNTGQRRDGPLVLIGSGLSSEQQKMLSELATILKAKKCAEFDGTVTHVIVPGDTVQSTLKCMLGILSGCWILKFEWVKACLQSKECEQEEKYEIPEGPQKSRLNREQLLPKLFDGCYFYLGGTFKHHPKDNLIKLVAAGGGQILIRKPKPDSDVTQSINTVAYHAKPDSDQRFCTQYIIYEDLSNHRPERVRQGKVWMAPSSWFIDCVMSFELLPLDN

Target ORF information:

RefSeq Version	XM_005202787.3
Organism	Bos taurus(cattle)
Definition	Bos taurus BRCA1 associated RING domain 1 (BARD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202787.3

ORF Insert Sequence:

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGAAGGAATC CTCATCAACC AGCCATTGCT CAGTAGTAAA CACTGGTCAG 
CGTCGGGATG GGCCTCTTGT ACTTATAGGC AGTGGACTTT CTTCCGAACA ACAGAAAATG 
CTCAGTGAGC TTGCAACGAT TCTTAAGGCT AAAAAATGTG CCGAGTTTGA CGGTACAGTA 
ACTCATGTCA TTGTTCCTGG TGATACAGTT CAAAGCACCC TGAAATGTAT GCTTGGAATT 
CTCAGTGGAT GCTGGATCCT AAAATTTGAA TGGGTGAAAG CATGTCTACA AAGCAAAGAA 
TGTGAACAGG AAGAAAAGTA TGAAATTCCT GAAGGACCAC AGAAAAGCAG GCTCAACAGA 
GAACAGCTGT TGCCAAAGCT GTTTGATGGA TGTTACTTCT ATTTGGGGGG AACCTTCAAA 
CACCATCCAA AGGACAACCT TATTAAGCTT GTCGCTGCCG GTGGGGGCCA GATCCTCATT 
AGAAAGCCCA AGCCAGACAG TGACGTGACT CAAAGCATCA ATACAGTCGC GTACCATGCC 
AAACCTGATT CTGATCAGCG CTTCTGCACA CAGTATATCA TCTATGAGGA CTTGTCTAAT 
CATCGCCCAG AAAGGGTTCG GCAGGGCAAA GTCTGGATGG CTCCTTCCAG CTGGTTTATA 
GATTGTGTGA TGTCCTTTGA GTTGCTCCCT CTTGACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BARD1 ( XM_005202787.2 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_005202844.1 Bos taurus BRCA1 associated RING domain 1 (BARD1), transcript variant X1, mRNA.

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CloneID	OBa16680
Clone ID Related Accession (Same CDS sequence)	XM_005202787.3 , XM_005202787.2
Accession Version	XM_005202787.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	BRCA1-associated RING domain protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGAAGGAATC CTCATCAACC AGCCATTGCT CAGTAGTAAA CACTGGTCAG 
CGTCGGGATG GGCCTCTTGT ACTTATAGGC AGTGGACTTT CTTCCGAACA ACAGAAAATG 
CTCAGTGAGC TTGCAACGAT TCTTAAGGCT AAAAAATGTG CCGAGTTTGA CGGTACAGTA 
ACTCATGTCA TTGTTCCTGG TGATACAGTT CAAAGCACCC TGAAATGTAT GCTTGGAATT 
CTCAGTGGAT GCTGGATCCT AAAATTTGAA TGGGTGAAAG CATGTCTACA AAGCAAAGAA 
TGTGAACAGG AAGAAAAGTA TGAAATTCCT GAAGGACCAC AGAAAAGCAG GCTCAACAGA 
GAACAGCTGT TGCCAAAGCT GTTTGATGGA TGTTACTTCT ATTTGGGGGG AACCTTCAAA 
CACCATCCAA AGGACAACCT TATTAAGCTT GTCGCTGCCG GTGGGGGCCA GATCCTCATT 
AGAAAGCCCA AGCCAGACAG TGACGTGACT CAAAGCATCA ATACAGTCGC GTACCATGCC 
AAACCTGATT CTGATCAGCG CTTCTGCACA CAGTATATCA TCTATGAGGA CTTGTCTAAT 
CATCGCCCAG AAAGGGTTCG GCAGGGCAAA GTCTGGATGG CTCCTTCCAG CTGGTTTATA 
GATTGTGTGA TGTCCTTTGA GTTGCTCCCT CTTGACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202844.1
CDS	133..2454
Translation	MQGNRQPRVRSGNQPHPAPAMKPAGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGSECPVCYTPAWIQDVKINRQLDSMIQLCSKLQNLLHDTDLSDLKEKTSRKSLFNDAQSKKNSIKMWFSPRSKKVRYTVSKLSVQTQPSVKNDENAQQTSMYEFVSPSPPVEVSEGPKKPSTRSRKKQKKKTLAEVNRKWNLEAEKEDGDLDCKGESKEKLVSFCSQPSVIANLQINGEIDLLASGSVTESECLDEVFLPLAEHIESPEIESKNEVVTPEKTVSANYLPSKKSLPSGRNGKRGRHNRISSPVSKRCRSSSQSTGGNSVKQTVLLENMPLLGCSSPPSNKLKVGDTLSRNSRTILDESISLSPGTPPSTLNSPSYRRMMCSPSATKLWPISHTAVKRNHRGETLLHIASIKGDIPSVEYLLQSGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAVKNGHVDIVKLLLTYGASREAVNIFGLRPVDYADSENMKSLLLLPEKKESSSTSHCSVVNTGQRRDGPLVLIGSGLSSEQQKMLSELATILKAKKCAEFDGTVTHVIVPGDTVQSTLKCMLGILSGCWILKFEWVKACLQSKECEQEEKYEIPEGPQKSRLNREQLLPKLFDGCYFYLGGTFKHHPKDNLIKLVAAGGGQILIRKPKPDSDVTQSINTVAYHAKPDSDQRFCTQYIIYEDLSNHRPERVRQGKVWMAPSSWFIDCVMSFELLPLDN

Target ORF information:

RefSeq Version	XM_005202787.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BRCA1 associated RING domain 1 (BARD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202787.2

ORF Insert Sequence:

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGAAGGAATC CTCATCAACC AGCCATTGCT CAGTAGTAAA CACTGGTCAG 
CGTCGGGATG GGCCTCTTGT ACTTATAGGC AGTGGACTTT CTTCCGAACA ACAGAAAATG 
CTCAGTGAGC TTGCAACGAT TCTTAAGGCT AAAAAATGTG CCGAGTTTGA CGGTACAGTA 
ACTCATGTCA TTGTTCCTGG TGATACAGTT CAAAGCACCC TGAAATGTAT GCTTGGAATT 
CTCAGTGGAT GCTGGATCCT AAAATTTGAA TGGGTGAAAG CATGTCTACA AAGCAAAGAA 
TGTGAACAGG AAGAAAAGTA TGAAATTCCT GAAGGACCAC AGAAAAGCAG GCTCAACAGA 
GAACAGCTGT TGCCAAAGCT GTTTGATGGA TGTTACTTCT ATTTGGGGGG AACCTTCAAA 
CACCATCCAA AGGACAACCT TATTAAGCTT GTCGCTGCCG GTGGGGGCCA GATCCTCATT 
AGAAAGCCCA AGCCAGACAG TGACGTGACT CAAAGCATCA ATACAGTCGC GTACCATGCC 
AAACCTGATT CTGATCAGCG CTTCTGCACA CAGTATATCA TCTATGAGGA CTTGTCTAAT 
CATCGCCCAG AAAGGGTTCG GCAGGGCAAA GTCTGGATGG CTCCTTCCAG CTGGTTTATA 
GATTGTGTGA TGTCCTTTGA GTTGCTCCCT CTTGACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BARD1 ( NM_001192701.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001179630.2 Bos taurus BRCA1 associated RING domain 1 (BARD1), mRNA.

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CloneID	OBa216288
Clone ID Related Accession (Same CDS sequence)	NM_001192701.2
Accession Version	NM_001192701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	BRCA1-associated RING domain protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Jun 30, 2017 this sequence version replaced NM_001192701.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145415, SAMN03145432 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGGAAGAGAT CTCTAGTCTC TCCCATTCTG TTGTAAACAC TGGTCAGCGT 
CGGGATGGGC CTCTTGTACT TATAGGCAGT GGACTTTCTT CCGAACAACA GAAAATGCTC 
AGTGAGCTTG CAACGATTCT TAAGGCTAAA AAATGTGCCG AGTTTGACGG TACAGTAACT 
CATGTCATTG TTCCTGGTGA TACAGTTCAA AGCACCCTGA AATGTATGCT TGGAATTCTC 
AGTGGATGCT GGATCCTAAA ATTTGAATGG GTGAAAGCAT GTCTACAAAG CAAAGAATGT 
GAACAGGAAG AAAAGTATGA AATTCCTGAA GGACCACAGA AAAGCAGGCT CAACAGAGAA 
CAGCTGTTGC CAAAGCTGTT TGATGGATGT TACTTCTATT TGGGGGGAAC CTTCAAACAC 
CATCCAAAGG ACAACCTTAT TAAGCTTGTC GCTGCCGGTG GGGGCCAGAT CCTCATTAGA 
AAGCCCAAGC CAGACAGTGA CGTGACTCAA AGCATCAATA CAGTCGCGTA CCATGCCAAA 
CCTGATTCTG ATCAGCGCTT CTGCACACAG TATATCATCT ATGAGGACTT GTCTAATCAT 
CGCCCAGAAA GGGTTCGGCA GGGCAAAGTC TGGATGGCTC CTTCCAGCTG GTTTATAGAT 
TGTGTGATGT CCTTTGAGTT GCTCCCTCTT GACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179630.2
CDS	112..2430
Translation	MQGNRQPRVRSGNQPHPAPAMKPAGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGSECPVCYTPAWIQDVKINRQLDSMIQLCSKLQNLLHDTDLSDLKEKTSRKSLFNDAQSKKNSIKMWFSPRSKKVRYTVSKLSVQTQPSVKNDENAQQTSMYEFVSPSPPVEVSEGPKKPSTRSRKKQKKKTLAEVNRKWNLEAEKEDGDLDCKGESKEKLVSFCSQPSVIANLQINGEIDLLASGSVTESECLDEVFLPLAEHIESPEIESKNEVVTPEKTVSANYLPSKKSLPSGRNGKRGRHNRISSPVSKRCRSSSQSTGGNSVKQTVLLENMPLLGCSSPPSNKLKVGDTLSRNSRTILDESISLSPGTPPSTLNSPSYRRMMCSPSATKLWPISHTAVKRNHRGETLLHIASIKGDIPSVEYLLQSGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAVKNGHVDIVKLLLTYGASREAVNIFGLRPVDYADSENMKSLLLLPEKEEISSLSHSVVNTGQRRDGPLVLIGSGLSSEQQKMLSELATILKAKKCAEFDGTVTHVIVPGDTVQSTLKCMLGILSGCWILKFEWVKACLQSKECEQEEKYEIPEGPQKSRLNREQLLPKLFDGCYFYLGGTFKHHPKDNLIKLVAAGGGQILIRKPKPDSDVTQSINTVAYHAKPDSDQRFCTQYIIYEDLSNHRPERVRQGKVWMAPSSWFIDCVMSFELLPLDN

Target ORF information:

RefSeq Version	NM_001192701.2
Organism	Bos taurus(cattle)
Definition	Bos taurus BRCA1 associated RING domain 1 (BARD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192701.2

ORF Insert Sequence:

ATGCAGGGGA ACCGGCAGCC GAGGGTCCGC TCCGGGAACC AGCCTCATCC CGCGCCCGCC 
ATGAAGCCGG CCGGTCGCGG GGCCTGGGCC CACAGCCGCG CCGCGCTTGA CCGGCTGGAG 
AAGCTGCTGC GCTGCTCACG CTGCACTAAT ATTCTGAGGG AGCCTGTGTG CTTAGGCGGA 
TGTGAGCATA TCTTCTGTAG TAATTGTGTA AGTGACTGCA TCGGAAGTGA ATGTCCAGTG 
TGTTATACCC CAGCCTGGAT ACAAGATGTG AAGATAAACA GACAGCTGGA CAGCATGATC 
CAGCTGTGTA GTAAGCTTCA GAATTTGCTG CACGACACTG ATCTTTCAGA TTTGAAAGAA 
AAAACATCTA GGAAGAGTTT ATTTAATGAT GCACAAAGTA AGAAGAATTC AATAAAAATG 
TGGTTTAGTC CTCGAAGTAA GAAAGTCAGA TATACTGTGA GTAAACTTTC AGTGCAAACC 
CAGCCTTCTG TGAAAAATGA TGAAAATGCT CAGCAGACCT CCATGTACGA ATTTGTCTCT 
CCGAGCCCTC CGGTGGAGGT TTCTGAGGGA CCTAAGAAGC CTTCCACAAG ATCTAGAAAA 
AAACAGAAAA AGAAAACTTT AGCTGAAGTC AACCGAAAAT GGAATTTAGA GGCAGAAAAG 
GAAGATGGTG ACCTTGACTG CAAAGGGGAA AGTAAGGAAA AGCTGGTATC CTTCTGTAGC 
CAACCATCTG TGATTGCTAA CCTGCAGATA AATGGTGAAA TAGACTTACT AGCAAGTGGG 
TCTGTAACAG AATCTGAATG CTTAGATGAA GTCTTTTTAC CATTGGCTGA GCATATAGAG 
TCTCCAGAAA TTGAGAGCAA GAATGAAGTA GTGACTCCTG AGAAGACAGT CTCTGCAAAC 
TATCTTCCTT CCAAGAAATC TTTGCCATCA GGTCGTAATG GGAAGCGTGG GCGTCACAAC 
AGAATTTCCA GTCCTGTTTC TAAGAGATGC AGAAGCAGCA GTCAGAGCAC TGGTGGAAAC 
TCTGTTAAGC AAACAGTGCT CTTAGAAAAC ATGCCATTGC TTGGCTGTTC TTCACCACCT 
TCAAACAAAC TTAAAGTTGG TGACACATTA AGTAGGAATA GCCGTACTAT ATTGGATGAA 
TCCATTAGCC TTTCACCAGG TACACCACCT TCTACACTGA ACAGTCCAAG TTACAGACGA 
ATGATGTGTA GCCCCTCAGC AACAAAGCTG TGGCCCATTA GCCATACAGC TGTGAAGAGA 
AATCATCGAG GAGAGACTTT GCTCCATATT GCTTCAATTA AGGGCGACAT CCCTTCTGTT 
GAATACCTCT TACAAAGTGG AAGCGACCCA AATGTTAAAG ACCATGCTGG ATGGACACCA 
TTGCATGAAG CCTGCAATCA CGGGCACCTG AAGGTGGTGG AGTTGCTGCT CCAGCACAAG 
GCGTTGGTGA ACACCACTGG GTATCAGAAC GACTCGCCTC TTCATGACGC AGTGAAGAAC 
GGGCATGTGG ACATTGTCAA GCTGTTACTC ACCTATGGCG CCTCCAGGGA GGCAGTCAAC 
ATATTTGGTC TGCGGCCTGT GGATTATGCA GACAGTGAAA ATATGAAATC ACTGTTGCTG 
CTACCGGAGA AGGAAGAGAT CTCTAGTCTC TCCCATTCTG TTGTAAACAC TGGTCAGCGT 
CGGGATGGGC CTCTTGTACT TATAGGCAGT GGACTTTCTT CCGAACAACA GAAAATGCTC 
AGTGAGCTTG CAACGATTCT TAAGGCTAAA AAATGTGCCG AGTTTGACGG TACAGTAACT 
CATGTCATTG TTCCTGGTGA TACAGTTCAA AGCACCCTGA AATGTATGCT TGGAATTCTC 
AGTGGATGCT GGATCCTAAA ATTTGAATGG GTGAAAGCAT GTCTACAAAG CAAAGAATGT 
GAACAGGAAG AAAAGTATGA AATTCCTGAA GGACCACAGA AAAGCAGGCT CAACAGAGAA 
CAGCTGTTGC CAAAGCTGTT TGATGGATGT TACTTCTATT TGGGGGGAAC CTTCAAACAC 
CATCCAAAGG ACAACCTTAT TAAGCTTGTC GCTGCCGGTG GGGGCCAGAT CCTCATTAGA 
AAGCCCAAGC CAGACAGTGA CGTGACTCAA AGCATCAATA CAGTCGCGTA CCATGCCAAA 
CCTGATTCTG ATCAGCGCTT CTGCACACAG TATATCATCT ATGAGGACTT GTCTAATCAT 
CGCCCAGAAA GGGTTCGGCA GGGCAAAGTC TGGATGGCTC CTTCCAGCTG GTTTATAGAT 
TGTGTGATGT CCTTTGAGTT GCTCCCTCTT GACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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BARD1 cDNA ORF clone, Bos taurus(Cattle)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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