UNC13B cDNA ORF clone, Bos taurus(cattle)

The following UNC13B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UNC13B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237129 XM_024995784.1
Latest version!
Bos taurus unc-13 homolog B (UNC13B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237130 XM_024995785.1
Latest version!
Bos taurus unc-13 homolog B (UNC13B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa91276 XM_015472527.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa216355 NM_001193239.2
Latest version!
Bos taurus unc-13 homolog B (UNC13B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa239199 NM_001193239.3
Latest version!
Bos taurus unc-13 homolog B (UNC13B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237125 XM_015472522.2
Latest version!
Bos taurus unc-13 homolog B (UNC13B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa237126 XM_024995782.1
Latest version!
Bos taurus unc-13 homolog B (UNC13B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa237127 XM_024995783.1
Latest version!
Bos taurus unc-13 homolog B (UNC13B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237128 XM_005210089.3
Latest version!
Bos taurus unc-13 homolog B (UNC13B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa91271 XM_015472521.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa91272 XM_015472523.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa91273 XM_015472524.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91274 XM_015472525.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa91275 XM_015472526.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa01673 NM_001193239.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa22042 XM_015472522.1
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa22045 XM_005210089.2
Latest version!
Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237129
    Clone ID Related Accession (Same CDS sequence) XM_024995784.1
    Accession Version XM_024995784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4224bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GGGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGATAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CATGCCACCT CTTCCTCCTG TTCGTCCATC
    TGTCCCTCTG TCCCTTGTAT CTGTCTGGGT GACTGTTTCT GGAGACAGTT GTGGAAACAT
    AATGAGGGGC CCCGCCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851552.1
    CDS81..4304
    Translation

    Target ORF information:

    RefSeq Version XM_024995784.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GGGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGATAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CATGCCACCT CTTCCTCCTG TTCGTCCATC
    TGTCCCTCTG TCCCTTGTAT CTGTCTGGGT GACTGTTTCT GGAGACAGTT GTGGAAACAT
    AATGAGGGGC CCCGCCCCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237130
    Clone ID Related Accession (Same CDS sequence) XM_024995785.1
    Accession Version XM_024995785.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCCCAGT GGCTTCCAGA AGGGCCAGCT GGAGGGCTCT ATGGCATTGA CAGCATGCCG 
    GATCTACGCA GAAAGAAGCC ACTGCCGCTT GTCAGCGACC TGTCACTGGT CCAGTCCCGG
    AAAGCAGGCA TTACTTCTGC AATGGCTACA CGCACCTCTC TCAAAGATGA AGAGCTGAAA
    TCACATGTGT ATAAGAAAAC CCTGCAGGCC TTAATCTATC CCATCTCGTG CACCACACCC
    CACAACTTTG AGGTGTGGTC AGCCACCACC CCCACCTACT GCTATGAGTG TGAAGGCCTG
    CTCTGGGGCA TTGCCCGGCA GGGCATGCGC TGCAGCGAGT GTGGAGTCAA GTGCCATGAG
    AAGTGCCAGG ACCTACTCAA TGCTGACTGC CTGCAGCGGG CTGCAGAAAA GAGCTCTAAA
    CATGGAGCGG AGGACCGGAC CCAGAACATC ATCATGGCCA TGAAGGACCG CATGAAGATC
    CGAGAGCGGA ATAAGCCAGA GATCTTTGAA GTTATCCGGG ATGTCTTCAA TGTGAGCAAA
    GTCGCCCATG TGCAACAAAT GAAAACAGTG AAGCAGAGTG TGTTGGATGG CACCTCCAAA
    TGGTCAGCCA AAATCACCAT CACTGTGGTG TGTGCCCAGG GCCTACAAGC CAAGGACAAG
    ACAGGATCCA GTGACCCTTA TGTGACTGTG CAAGTGGGCA AAACCAAGAA GCGTACCAAG
    ACCATTTTTG GAAACCTGAA TCCTGTTTGG GAGGAGAAGT TCCATTTTGA GTGCCACAAC
    TCTTCTGACC GCATTAAGGT ACGTGTGTGG GATGAGGATG ATGACATCAA GTCACGAGTA
    AAGCAGCGGC TAAAGCGAGA GTCTGATGAT TTCCTCGGCC AAACCATCAT TGAGGTTCGA
    ACACTGAGTG GCGAAATGGA CGTCTGGTAC AACTTGGAGA AGAGGACAGA CAAGTCAGCC
    GTCTCGGGGG CCATCCGACT GCAAATCAGC GTGGAGATCA AGGGAGAAGA GAAGGTAGCC
    CCATATCATG TGCAGTACAC GTGTCTCCAT GAGAACCTCT TCCATTACCT CACGGACATT
    CAGGGCAGCG GAGGAGTCCA GATCCCTGAG GCTCGAGGAG ATGATGCATG GAAGGTGTAC
    TTTGATGAGA CTGCCCAGGA AATTGTGGAT GAATTTGCCA TGCGCTATGG CATTGAGTCC
    ATATATCAGG CCATGACGCA TTTTGCATGT TTGTCATCCA AGTACATGTG TCCTGGTGTG
    CCAGCAGTGA TGAGCACCTT ACTGGCCAAC ATCAATGCCT ACTATGCCCA CACAACTGCC
    TCCACCAATG TCTCTGCATC TGATCGTTTT GCAGCCTCCA ACTTTGGGAA AGAGAGATTT
    GTGAAACTTC TGGACCAGCT GCACAACTCA CTGAGGATTG ACCTCTCTAC CTACAGGAAT
    AATTTCCCTG CTGGGAGCCC TGAGCGACTT CAGGACTTAA AGTCCACAGT GGATTTGCTG
    ACCAGCATTA CTTTCTTCCG AATGAAGGTG CAAGAACTTC AGAGCCCTCC AAGAGCCAGC
    CAGGTAGTAA AAGATTGTGT GAAGGCCTGT TTGAACTCAA CATATGAGTA TATCTTCAAC
    AACTGCCATG ACTTGTACAG CCGCCAGTAC CAGCTGAATC AGGAGCTGCC TCCAGAAGAA
    CAGGGGCCCA GCATTAGGAA CCTGGATTTC TGGCCCAAAC TCATCACACT CATTGTGTCA
    ATCATAGAGG AAGATAAGAA TTCCTACACC CTCGTTCTGA ACCAGTTTCC CCAGGAGTTG
    AATGTGGGAA AAGTCAGCGC AGAAGTGATG TGGCAACTCT TTGCCCAAGA CATGAAATAT
    GCACTGGAGG AGCATGAGAA AGACAGGCTG TGTAAGAGTG CTGACTACAT GAACCTGCAC
    TTCAAGGTGA AGTGGCTCCA CAATGAATAC GTGCAGGATC TGCCTGCCCT CCAGGGCCAG
    GTGCCCGAAT ACCCAGCGTG GTTTGAGCAG TTTGTCCTGC AGTGGCTGGA CGAGAATGAG
    GATGTGTCCT TGGAATTCCT GCGTGGGGCC TTGGAACGAG ATAAGAAGGA TGGGTTCCAG
    CAGACATCAG AGCACGCGCT CTTCTCTTGC TCTGTGGTGG ACGTCTTCAC ACAGCTCAAT
    CAGAGCTTTG AGATCATCCG GAAATTGGAA TGCCCAGACC CCAACATCCT TGCCCACTAC
    ATGAGGAGAT TTTCTAAGAC CATCGGGAAG GTGCTCATGG AATATGCAGA CATCTTATCA
    AAGAACTTCC CAGCTTACTG CACAAAGGAG AAAATGCCTT GCATCCTGAT GAACAACATG
    CAGCAGCTGA GGGTGCAGCT GGAGAAGATG TTTGAGGCCA TGGGAGGCAA GGAGCTGGAC
    CCTGAAACTG CAGACAGTCT GAAGGAGCTT CAGGTGAAAC TGAATACAGT TTTGGATGAG
    CTCAGCATGG TGTTTGGAAA CAGTTTCCAG GTGCGGATTG ATGAGTGCGT GCGACAAATG
    GCCGACATCC TGAGCCAGGT GCGGGGCCCA GGGAACGCAT CCCCCAACGC ACGGGCCTCG
    GTGACTCAGG ATGCAGACAG TGTGCTCCGG CCTCTCATGG ACTTCCTGGA CGGCAACCTC
    ACACTTTTTG CCACTGTGTG TGAGAAGACG GTCCTGAAGC GGGTACTGAA GGAACTCTGG
    CGGGTGGTAA TGAACACAAT GGAAAGGATG ATTGTTCTGC CTCCACTTAC TGACCAGACG
    GGCACCCAGC TGATCTTCAC TGCTGCCAAG GAGCTGAGCC AACTTTCCAA ACTCAAGGAC
    CACATGGTGC GAGAGGAAAC ACGGAATCTG ACTCCAAAGC AGTGTGCCGT CCTTGACCTC
    GCCCTGGACA CCATCAAGCA ATACTTTCAC GCCGGAGGCA ACGGGCTGAA GAAAACCTTC
    CTGGAGAAGA GCCCAGATCT GCAGTCCCTG CGCTATGCCC TATCTCTGTA CACACAGACC
    ACAGACACCC TCATCAAGAC CTTTGTGCGC TCACAGACTG CCCAGGTGCA TGATGGGAAA
    GGTATTAGGT TTACTGCTAA TGAGGACATT CAGCCGGAAA AGGGGTCCGG TGTGGATGAT
    CCTGTGGGAG AAGTCTCTAT TCAGGTGGAC TTGTTTACAC ATCCTGGAAC TGGGGAGCAC
    AAGGTCACAG TGAAAGTGGT GGCTGCCAAT GACCTCAAGT GGCAGACAGC AGGTATGTTC
    CGGCCCTTTG TCGAAGTCAC CATGGTTGGC CCTCACCAAA GTGATAAAAA GAGGAAGTTC
    ACAACCAAGT CAAAAAGTAA CAACTGGGCT CCCAAGTACA ACGAGACGTT TCACTTCCTC
    CTGGGAAATG AAGAGGGGCC AGAAGCCTAT GAGTTGCAGA TATGCGTGAA GGATTACTGC
    TTTGCCCGGG AGGACCATGT GCTGGGGCTG GCTGTAATGC CTCTGAGGGA TGTGGCAGCC
    AAGGGCAGCT GTGCTTGCTG GTGCCCCTTG GGCCGTAAGA TCCACATGGA TGAGACAGGC
    GTGACCATTC TCCGGATTCT GTCTCAGAGG AGCAATGACG AAGTGGCCCG GGAGTTTGTG
    AAGCTCAAAT CAGAGTCTCG TTCTATGGAG GAGGGGAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851553.1
    CDS96..3737
    Translation

    Target ORF information:

    RefSeq Version XM_024995785.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCCCAGT GGCTTCCAGA AGGGCCAGCT GGAGGGCTCT ATGGCATTGA CAGCATGCCG 
    GATCTACGCA GAAAGAAGCC ACTGCCGCTT GTCAGCGACC TGTCACTGGT CCAGTCCCGG
    AAAGCAGGCA TTACTTCTGC AATGGCTACA CGCACCTCTC TCAAAGATGA AGAGCTGAAA
    TCACATGTGT ATAAGAAAAC CCTGCAGGCC TTAATCTATC CCATCTCGTG CACCACACCC
    CACAACTTTG AGGTGTGGTC AGCCACCACC CCCACCTACT GCTATGAGTG TGAAGGCCTG
    CTCTGGGGCA TTGCCCGGCA GGGCATGCGC TGCAGCGAGT GTGGAGTCAA GTGCCATGAG
    AAGTGCCAGG ACCTACTCAA TGCTGACTGC CTGCAGCGGG CTGCAGAAAA GAGCTCTAAA
    CATGGAGCGG AGGACCGGAC CCAGAACATC ATCATGGCCA TGAAGGACCG CATGAAGATC
    CGAGAGCGGA ATAAGCCAGA GATCTTTGAA GTTATCCGGG ATGTCTTCAA TGTGAGCAAA
    GTCGCCCATG TGCAACAAAT GAAAACAGTG AAGCAGAGTG TGTTGGATGG CACCTCCAAA
    TGGTCAGCCA AAATCACCAT CACTGTGGTG TGTGCCCAGG GCCTACAAGC CAAGGACAAG
    ACAGGATCCA GTGACCCTTA TGTGACTGTG CAAGTGGGCA AAACCAAGAA GCGTACCAAG
    ACCATTTTTG GAAACCTGAA TCCTGTTTGG GAGGAGAAGT TCCATTTTGA GTGCCACAAC
    TCTTCTGACC GCATTAAGGT ACGTGTGTGG GATGAGGATG ATGACATCAA GTCACGAGTA
    AAGCAGCGGC TAAAGCGAGA GTCTGATGAT TTCCTCGGCC AAACCATCAT TGAGGTTCGA
    ACACTGAGTG GCGAAATGGA CGTCTGGTAC AACTTGGAGA AGAGGACAGA CAAGTCAGCC
    GTCTCGGGGG CCATCCGACT GCAAATCAGC GTGGAGATCA AGGGAGAAGA GAAGGTAGCC
    CCATATCATG TGCAGTACAC GTGTCTCCAT GAGAACCTCT TCCATTACCT CACGGACATT
    CAGGGCAGCG GAGGAGTCCA GATCCCTGAG GCTCGAGGAG ATGATGCATG GAAGGTGTAC
    TTTGATGAGA CTGCCCAGGA AATTGTGGAT GAATTTGCCA TGCGCTATGG CATTGAGTCC
    ATATATCAGG CCATGACGCA TTTTGCATGT TTGTCATCCA AGTACATGTG TCCTGGTGTG
    CCAGCAGTGA TGAGCACCTT ACTGGCCAAC ATCAATGCCT ACTATGCCCA CACAACTGCC
    TCCACCAATG TCTCTGCATC TGATCGTTTT GCAGCCTCCA ACTTTGGGAA AGAGAGATTT
    GTGAAACTTC TGGACCAGCT GCACAACTCA CTGAGGATTG ACCTCTCTAC CTACAGGAAT
    AATTTCCCTG CTGGGAGCCC TGAGCGACTT CAGGACTTAA AGTCCACAGT GGATTTGCTG
    ACCAGCATTA CTTTCTTCCG AATGAAGGTG CAAGAACTTC AGAGCCCTCC AAGAGCCAGC
    CAGGTAGTAA AAGATTGTGT GAAGGCCTGT TTGAACTCAA CATATGAGTA TATCTTCAAC
    AACTGCCATG ACTTGTACAG CCGCCAGTAC CAGCTGAATC AGGAGCTGCC TCCAGAAGAA
    CAGGGGCCCA GCATTAGGAA CCTGGATTTC TGGCCCAAAC TCATCACACT CATTGTGTCA
    ATCATAGAGG AAGATAAGAA TTCCTACACC CTCGTTCTGA ACCAGTTTCC CCAGGAGTTG
    AATGTGGGAA AAGTCAGCGC AGAAGTGATG TGGCAACTCT TTGCCCAAGA CATGAAATAT
    GCACTGGAGG AGCATGAGAA AGACAGGCTG TGTAAGAGTG CTGACTACAT GAACCTGCAC
    TTCAAGGTGA AGTGGCTCCA CAATGAATAC GTGCAGGATC TGCCTGCCCT CCAGGGCCAG
    GTGCCCGAAT ACCCAGCGTG GTTTGAGCAG TTTGTCCTGC AGTGGCTGGA CGAGAATGAG
    GATGTGTCCT TGGAATTCCT GCGTGGGGCC TTGGAACGAG ATAAGAAGGA TGGGTTCCAG
    CAGACATCAG AGCACGCGCT CTTCTCTTGC TCTGTGGTGG ACGTCTTCAC ACAGCTCAAT
    CAGAGCTTTG AGATCATCCG GAAATTGGAA TGCCCAGACC CCAACATCCT TGCCCACTAC
    ATGAGGAGAT TTTCTAAGAC CATCGGGAAG GTGCTCATGG AATATGCAGA CATCTTATCA
    AAGAACTTCC CAGCTTACTG CACAAAGGAG AAAATGCCTT GCATCCTGAT GAACAACATG
    CAGCAGCTGA GGGTGCAGCT GGAGAAGATG TTTGAGGCCA TGGGAGGCAA GGAGCTGGAC
    CCTGAAACTG CAGACAGTCT GAAGGAGCTT CAGGTGAAAC TGAATACAGT TTTGGATGAG
    CTCAGCATGG TGTTTGGAAA CAGTTTCCAG GTGCGGATTG ATGAGTGCGT GCGACAAATG
    GCCGACATCC TGAGCCAGGT GCGGGGCCCA GGGAACGCAT CCCCCAACGC ACGGGCCTCG
    GTGACTCAGG ATGCAGACAG TGTGCTCCGG CCTCTCATGG ACTTCCTGGA CGGCAACCTC
    ACACTTTTTG CCACTGTGTG TGAGAAGACG GTCCTGAAGC GGGTACTGAA GGAACTCTGG
    CGGGTGGTAA TGAACACAAT GGAAAGGATG ATTGTTCTGC CTCCACTTAC TGACCAGACG
    GGCACCCAGC TGATCTTCAC TGCTGCCAAG GAGCTGAGCC AACTTTCCAA ACTCAAGGAC
    CACATGGTGC GAGAGGAAAC ACGGAATCTG ACTCCAAAGC AGTGTGCCGT CCTTGACCTC
    GCCCTGGACA CCATCAAGCA ATACTTTCAC GCCGGAGGCA ACGGGCTGAA GAAAACCTTC
    CTGGAGAAGA GCCCAGATCT GCAGTCCCTG CGCTATGCCC TATCTCTGTA CACACAGACC
    ACAGACACCC TCATCAAGAC CTTTGTGCGC TCACAGACTG CCCAGGTGCA TGATGGGAAA
    GGTATTAGGT TTACTGCTAA TGAGGACATT CAGCCGGAAA AGGGGTCCGG TGTGGATGAT
    CCTGTGGGAG AAGTCTCTAT TCAGGTGGAC TTGTTTACAC ATCCTGGAAC TGGGGAGCAC
    AAGGTCACAG TGAAAGTGGT GGCTGCCAAT GACCTCAAGT GGCAGACAGC AGGTATGTTC
    CGGCCCTTTG TCGAAGTCAC CATGGTTGGC CCTCACCAAA GTGATAAAAA GAGGAAGTTC
    ACAACCAAGT CAAAAAGTAA CAACTGGGCT CCCAAGTACA ACGAGACGTT TCACTTCCTC
    CTGGGAAATG AAGAGGGGCC AGAAGCCTAT GAGTTGCAGA TATGCGTGAA GGATTACTGC
    TTTGCCCGGG AGGACCATGT GCTGGGGCTG GCTGTAATGC CTCTGAGGGA TGTGGCAGCC
    AAGGGCAGCT GTGCTTGCTG GTGCCCCTTG GGCCGTAAGA TCCACATGGA TGAGACAGGC
    GTGACCATTC TCCGGATTCT GTCTCAGAGG AGCAATGACG AAGTGGCCCG GGAGTTTGTG
    AAGCTCAAAT CAGAGTCTCG TTCTATGGAG GAGGGGAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91276
    Clone ID Related Accession (Same CDS sequence) XM_015472527.1
    Accession Version XM_015472527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCCCAGT GGCTTCCAGA AGGGCCAGCT GGAGGGCTCT ATGGCATTGA CAGCATGCCG 
    GATCTACGCA GAAAGAAGCC ACTGCCGCTT GTCAGCGACC TGGCTATGTC ACTGGTCCAG
    TCCCGGAAAG CAGGCATTAC TTCTGCAATG GCTACACGCA CCTCTCTCAA AGATGAAGAG
    CTGAAATCAC ATGTGTATAA GAAAACCCTG CAGGCCTTAA TCTATCCCAT CTCGTGCACC
    ACACCCCACA ACTTTGAGGT GTGGTCAGCC ACCACCCCCA CCTACTGCTA TGAGTGTGAA
    GGCCTGCTCT GGGGCATTGC CCGGCAGGGC ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC
    CATGAGAAGT GCCAGGACCT ACTCAATGCT GACTGCCTGC AGCGGGCTGC AGAAAAGAGC
    TCTAAACATG GAGCGGAGGA CCGGACCCAG AACATCATCA TGGCCATGAA GGACCGCATG
    AAGATCCGAG AGCGGAATAA GCCAGAGATC TTTGAAGTTA TCCGGGATGT CTTCAATGTG
    AGCAAAGTCG CCCATGTGCA ACAAATGAAA ACAGTGAAGC AGAGTGTGTT GGATGGCACC
    TCCAAATGGT CAGCCAAAAT CACCATCACT GTGGTGTGTG CCCAGGGCCT ACAAGCCAAG
    GACAAGACAG GATCCAGTGA CCCTTATGTG ACTGTGCAAG TGGGCAAAAC CAAGAAGCGT
    ACCAAGACCA TTTTTGGAAA CCTGAATCCT GTTTGGGAGG AGAAGTTCCA TTTTGAGTGC
    CACAACTCTT CTGACCGCAT TAAGGTACGT GTGTGGGATG AGGATGATGA CATCAAGTCA
    CGAGTAAAGC AGCGGCTAAA GCGAGAGTCT GATGATTTCC TCGGCCAAAC CATCATTGAG
    GTTCGAACAC TGAGTGGCGA AATGGACGTC TGGTACAACT TGGAGAAGAG GACAGACAAG
    TCAGCCGTCT CGGGGGCCAT CCGACTGCAA ATCAGCGTGG AGATCAAGGG AGAAGAGAAG
    GTAGCCCCAT ATCATGTGCA GTACACGTGT CTCCATGAGA ACCTCTTCCA TTACCTCACG
    GACATTCAGG GCAGCGGAGG AGTCCAGATC CCTGAGGCTC GAGGAGATGA TGCATGGAAG
    GTGTACTTTG ATGAGACTGC CCAGGAAATT GTGGATGAAT TTGCCATGCG CTATGGCATT
    GAGTCCATAT ATCAGGCCAT GACGCATTTT GCATGTTTGT CATCCAAGTA CATGTGTCCT
    GGTGTGCCAG CAGTGATGAG CACCTTACTG GCCAACATCA ATGCCTACTA TGCCCACACA
    ACTGCCTCCA CCAATGTCTC TGCATCTGAT CGTTTTGCAG CCTCCAACTT TGGGAAAGAG
    AGATTTGTGA AACTTCTGGA CCAGCTGCAC AACTCACTGA GGATTGACCT CTCTACCTAC
    AGGAATAATT TCCCTGCTGG GAGCCCTGAG CGACTTCAGG ACTTAAAGTC CACAGTGGAT
    TTGCTGACCA GCATTACTTT CTTCCGAATG AAGGTGCAAG AACTTCAGAG CCCTCCAAGA
    GCCAGCCAGG TAGTAAAAGA TTGTGTGAAG GCCTGTTTGA ACTCAACATA TGAGTATATC
    TTCAACAACT GCCATGACTT GTACAGCCGC CAGTACCAGC TGAATCAGGA GCTGCCTCCA
    GAAGAACAGG GGCCCAGCAT TAGGAACCTG GATTTCTGGC CCAAACTCAT CACACTCATT
    GTGTCAATCA TAGAGGAAGA TAAGAATTCC TACACCCTCG TTCTGAACCA GTTTCCCCAG
    GAGTTGAATG TGGGAAAAGT CAGCGCAGAA GTGATGTGGC AACTCTTTGC CCAAGACATG
    AAATATGCAC TGGAGGAGCA TGAGAAAGAC AGGCTGTGTA AGAGTGCTGA CTACATGAAC
    CTGCACTTCA AGGTGAAGTG GCTCCACAAT GAATACGTGC AGGATCTGCC TGCCCTCCAG
    GGCCAGGTGC CCGAATACCC AGCGTGGTTT GAGCAGTTTG TCCTGCAGTG GCTGGACGAG
    AATGAGGATG TGTCCTTGGA ATTCCTGCGT GGGGCCTTGG AACGAGATAA GAAGGATGGG
    TTCCAGCAGA CATCAGAGCA CGCGCTCTTC TCTTGCTCTG TGGTGGACGT CTTCACACAG
    CTCAATCAGA GCTTTGAGAT CATCCGGAAA TTGGAATGCC CAGACCCCAA CATCCTTGCC
    CACTACATGA GGAGATTTTC TAAGACCATC GGGAAGGTGC TCATGGAATA TGCAGACATC
    TTATCAAAGA ACTTCCCAGC TTACTGCACA AAGGAGAAAA TGCCTTGCAT CCTGATGAAC
    AACATGCAGC AGCTGAGGGT GCAGCTGGAG AAGATGTTTG AGGCCATGGG AGGCAAGGAG
    CTGGACCCTG AAACTGCAGA CAGTCTGAAG GAGCTTCAGG TGAAACTGAA TACAGTTTTG
    GATGAGCTCA GCATGGTGTT TGGAAACAGT TTCCAGGTGC GGATTGATGA GTGCGTGCGA
    CAAATGGCCG ACATCCTGAG CCAGGTGCGG GGCCCAGGGA ACGCATCCCC CAACGCACGG
    GCCTCGGTGA CTCAGGATGC AGACAGTGTG CTCCGGCCTC TCATGGACTT CCTGGACGGC
    AACCTCACAC TTTTTGCCAC TGTGTGTGAG AAGACGGTCC TGAAGCGGGT ACTGAAGGAA
    CTCTGGCGGG TGGTAATGAA CACAATGGAA AGGATGATTG TTCTGCCTCC ACTTACTGAC
    CAGACGGGCA CCCAGCTGAT CTTCACTGCT GCCAAGGAGC TGAGCCAACT TTCCAAACTC
    AAGGACCACA TGGTGCGAGA GGAAACACGG AATCTGACTC CAAAGCAGTG TGCCGTCCTT
    GACCTCGCCC TGGACACCAT CAAGCAATAC TTTCACGCCG GAGGCAACGG GCTGAAGAAA
    ACCTTCCTGG AGAAGAGCCC AGATCTGCAG TCCCTGCGCT ATGCCCTATC TCTGTACACA
    CAGACCACAG ACACCCTCAT CAAGACCTTT GTGCGCTCAC AGACTGCCCA GGTGCATGAT
    GGGAAAGGTA TTAGGTTTAC TGCTAATGAG GACATTCAGC CGGAAAAGGG GTCCGGTGTG
    GATGATCCTG TGGGAGAAGT CTCTATTCAG GTGGACTTGT TTACACATCC TGGAACTGGG
    GAGCACAAGG TCACAGTGAA AGTGGTGGCT GCCAATGACC TCAAGTGGCA GACAGCAGGT
    ATGTTCCGGC CCTTTGTCGA AGTCACCATG GTTGGCCCTC ACCAAAGTGA TAAAAAGAGG
    AAGTTCACAA CCAAGTCAAA AAGTAACAAC TGGGCTCCCA AGTACAACGA GACGTTTCAC
    TTCCTCCTGG GAAATGAAGA GGGGCCAGAA GCCTATGAGT TGCAGATATG CGTGAAGGAT
    TACTGCTTTG CCCGGGAGGA CCGTGTGCTG GGGCTGGCTG TAATGCCTCT GAGGGATGTG
    GCAGCCAAGG GCAGCTGTGC TTGCTGGTGC CCCTTGGGCC GTAAGATCCA CATGGATGAG
    ACAGGCGTGA CCATTCTCCG GATTCTGTCT CAGAGGAGCA ATGACGAAGT GGCCCGGGAG
    TTTGTGAAGC TCAAATCAGA GTCTCGTTCT ATGGAGGAGG GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328013.1
    CDS263..3910
    Translation

    Target ORF information:

    RefSeq Version XM_015472527.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCCCAGT GGCTTCCAGA AGGGCCAGCT GGAGGGCTCT ATGGCATTGA CAGCATGCCG 
    GATCTACGCA GAAAGAAGCC ACTGCCGCTT GTCAGCGACC TGGCTATGTC ACTGGTCCAG
    TCCCGGAAAG CAGGCATTAC TTCTGCAATG GCTACACGCA CCTCTCTCAA AGATGAAGAG
    CTGAAATCAC ATGTGTATAA GAAAACCCTG CAGGCCTTAA TCTATCCCAT CTCGTGCACC
    ACACCCCACA ACTTTGAGGT GTGGTCAGCC ACCACCCCCA CCTACTGCTA TGAGTGTGAA
    GGCCTGCTCT GGGGCATTGC CCGGCAGGGC ATGCGCTGCA GCGAGTGTGG AGTCAAGTGC
    CATGAGAAGT GCCAGGACCT ACTCAATGCT GACTGCCTGC AGCGGGCTGC AGAAAAGAGC
    TCTAAACATG GAGCGGAGGA CCGGACCCAG AACATCATCA TGGCCATGAA GGACCGCATG
    AAGATCCGAG AGCGGAATAA GCCAGAGATC TTTGAAGTTA TCCGGGATGT CTTCAATGTG
    AGCAAAGTCG CCCATGTGCA ACAAATGAAA ACAGTGAAGC AGAGTGTGTT GGATGGCACC
    TCCAAATGGT CAGCCAAAAT CACCATCACT GTGGTGTGTG CCCAGGGCCT ACAAGCCAAG
    GACAAGACAG GATCCAGTGA CCCTTATGTG ACTGTGCAAG TGGGCAAAAC CAAGAAGCGT
    ACCAAGACCA TTTTTGGAAA CCTGAATCCT GTTTGGGAGG AGAAGTTCCA TTTTGAGTGC
    CACAACTCTT CTGACCGCAT TAAGGTACGT GTGTGGGATG AGGATGATGA CATCAAGTCA
    CGAGTAAAGC AGCGGCTAAA GCGAGAGTCT GATGATTTCC TCGGCCAAAC CATCATTGAG
    GTTCGAACAC TGAGTGGCGA AATGGACGTC TGGTACAACT TGGAGAAGAG GACAGACAAG
    TCAGCCGTCT CGGGGGCCAT CCGACTGCAA ATCAGCGTGG AGATCAAGGG AGAAGAGAAG
    GTAGCCCCAT ATCATGTGCA GTACACGTGT CTCCATGAGA ACCTCTTCCA TTACCTCACG
    GACATTCAGG GCAGCGGAGG AGTCCAGATC CCTGAGGCTC GAGGAGATGA TGCATGGAAG
    GTGTACTTTG ATGAGACTGC CCAGGAAATT GTGGATGAAT TTGCCATGCG CTATGGCATT
    GAGTCCATAT ATCAGGCCAT GACGCATTTT GCATGTTTGT CATCCAAGTA CATGTGTCCT
    GGTGTGCCAG CAGTGATGAG CACCTTACTG GCCAACATCA ATGCCTACTA TGCCCACACA
    ACTGCCTCCA CCAATGTCTC TGCATCTGAT CGTTTTGCAG CCTCCAACTT TGGGAAAGAG
    AGATTTGTGA AACTTCTGGA CCAGCTGCAC AACTCACTGA GGATTGACCT CTCTACCTAC
    AGGAATAATT TCCCTGCTGG GAGCCCTGAG CGACTTCAGG ACTTAAAGTC CACAGTGGAT
    TTGCTGACCA GCATTACTTT CTTCCGAATG AAGGTGCAAG AACTTCAGAG CCCTCCAAGA
    GCCAGCCAGG TAGTAAAAGA TTGTGTGAAG GCCTGTTTGA ACTCAACATA TGAGTATATC
    TTCAACAACT GCCATGACTT GTACAGCCGC CAGTACCAGC TGAATCAGGA GCTGCCTCCA
    GAAGAACAGG GGCCCAGCAT TAGGAACCTG GATTTCTGGC CCAAACTCAT CACACTCATT
    GTGTCAATCA TAGAGGAAGA TAAGAATTCC TACACCCTCG TTCTGAACCA GTTTCCCCAG
    GAGTTGAATG TGGGAAAAGT CAGCGCAGAA GTGATGTGGC AACTCTTTGC CCAAGACATG
    AAATATGCAC TGGAGGAGCA TGAGAAAGAC AGGCTGTGTA AGAGTGCTGA CTACATGAAC
    CTGCACTTCA AGGTGAAGTG GCTCCACAAT GAATACGTGC AGGATCTGCC TGCCCTCCAG
    GGCCAGGTGC CCGAATACCC AGCGTGGTTT GAGCAGTTTG TCCTGCAGTG GCTGGACGAG
    AATGAGGATG TGTCCTTGGA ATTCCTGCGT GGGGCCTTGG AACGAGATAA GAAGGATGGG
    TTCCAGCAGA CATCAGAGCA CGCGCTCTTC TCTTGCTCTG TGGTGGACGT CTTCACACAG
    CTCAATCAGA GCTTTGAGAT CATCCGGAAA TTGGAATGCC CAGACCCCAA CATCCTTGCC
    CACTACATGA GGAGATTTTC TAAGACCATC GGGAAGGTGC TCATGGAATA TGCAGACATC
    TTATCAAAGA ACTTCCCAGC TTACTGCACA AAGGAGAAAA TGCCTTGCAT CCTGATGAAC
    AACATGCAGC AGCTGAGGGT GCAGCTGGAG AAGATGTTTG AGGCCATGGG AGGCAAGGAG
    CTGGACCCTG AAACTGCAGA CAGTCTGAAG GAGCTTCAGG TGAAACTGAA TACAGTTTTG
    GATGAGCTCA GCATGGTGTT TGGAAACAGT TTCCAGGTGC GGATTGATGA GTGCGTGCGA
    CAAATGGCCG ACATCCTGAG CCAGGTGCGG GGCCCAGGGA ACGCATCCCC CAACGCACGG
    GCCTCGGTGA CTCAGGATGC AGACAGTGTG CTCCGGCCTC TCATGGACTT CCTGGACGGC
    AACCTCACAC TTTTTGCCAC TGTGTGTGAG AAGACGGTCC TGAAGCGGGT ACTGAAGGAA
    CTCTGGCGGG TGGTAATGAA CACAATGGAA AGGATGATTG TTCTGCCTCC ACTTACTGAC
    CAGACGGGCA CCCAGCTGAT CTTCACTGCT GCCAAGGAGC TGAGCCAACT TTCCAAACTC
    AAGGACCACA TGGTGCGAGA GGAAACACGG AATCTGACTC CAAAGCAGTG TGCCGTCCTT
    GACCTCGCCC TGGACACCAT CAAGCAATAC TTTCACGCCG GAGGCAACGG GCTGAAGAAA
    ACCTTCCTGG AGAAGAGCCC AGATCTGCAG TCCCTGCGCT ATGCCCTATC TCTGTACACA
    CAGACCACAG ACACCCTCAT CAAGACCTTT GTGCGCTCAC AGACTGCCCA GGTGCATGAT
    GGGAAAGGTA TTAGGTTTAC TGCTAATGAG GACATTCAGC CGGAAAAGGG GTCCGGTGTG
    GATGATCCTG TGGGAGAAGT CTCTATTCAG GTGGACTTGT TTACACATCC TGGAACTGGG
    GAGCACAAGG TCACAGTGAA AGTGGTGGCT GCCAATGACC TCAAGTGGCA GACAGCAGGT
    ATGTTCCGGC CCTTTGTCGA AGTCACCATG GTTGGCCCTC ACCAAAGTGA TAAAAAGAGG
    AAGTTCACAA CCAAGTCAAA AAGTAACAAC TGGGCTCCCA AGTACAACGA GACGTTTCAC
    TTCCTCCTGG GAAATGAAGA GGGGCCAGAA GCCTATGAGT TGCAGATATG CGTGAAGGAT
    TACTGCTTTG CCCGGGAGGA CCGTGTGCTG GGGCTGGCTG TAATGCCTCT GAGGGATGTG
    GCAGCCAAGG GCAGCTGTGC TTGCTGGTGC CCCTTGGGCC GTAAGATCCA CATGGATGAG
    ACAGGCGTGA CCATTCTCCG GATTCTGTCT CAGAGGAGCA ATGACGAAGT GGCCCGGGAG
    TTTGTGAAGC TCAAATCAGA GTCTCGTTCT ATGGAGGAGG GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216355
    Clone ID Related Accession (Same CDS sequence) NM_001193239.2
    Accession Version NM_001193239.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02023126.1, DAAA02023127.1 and DAAA02023128.1. On Jun 30, 2017 this sequence version replaced NM_001193239.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403657, SAMN02822088 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC GTGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180168.1
    CDS336..5111
    Translation

    Target ORF information:

    RefSeq Version NM_001193239.2
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC GTGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239199
    Clone ID Related Accession (Same CDS sequence) NM_001193239.3
    Accession Version NM_001193239.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-24
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000008.1. On May 29, 2018 this sequence version replaced NM_001193239.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GGVH01137099.1, GGVA01072473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC ATGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180168.2
    CDS336..5111
    Translation

    Target ORF information:

    RefSeq Version NM_001193239.3
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193239.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC ATGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237125
    Clone ID Related Accession (Same CDS sequence) XM_015472522.2
    Accession Version XM_015472522.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GGGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGATAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA
    GTCAGCGCAG AAGTGATGTG GCAACTCTTT GCCCAAGACA TGAAATATGC ACTGGAGGAG
    CATGAGAAAG ACAGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG
    TGGCTCCACA ATGAATACGT GCAGGATCTG CCTGCCCTCC AGGGCCAGGT GCCCGAATAC
    CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG TGGCTGGACG AGAATGAGGA TGTGTCCTTG
    GAATTCCTGC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG
    CACGCGCTCT TCTCTTGCTC TGTGGTGGAC GTCTTCACAC AGCTCAATCA GAGCTTTGAG
    ATCATCCGGA AATTGGAATG CCCAGACCCC AACATCCTTG CCCACTACAT GAGGAGATTT
    TCTAAGACCA TCGGGAAGGT GCTCATGGAA TATGCAGACA TCTTATCAAA GAACTTCCCA
    GCTTACTGCA CAAAGGAGAA AATGCCTTGC ATCCTGATGA ACAACATGCA GCAGCTGAGG
    GTGCAGCTGG AGAAGATGTT TGAGGCCATG GGAGGCAAGG AGCTGGACCC TGAAACTGCA
    GACAGTCTGA AGGAGCTTCA GGTGAAACTG AATACAGTTT TGGATGAGCT CAGCATGGTG
    TTTGGAAACA GTTTCCAGGT GCGGATTGAT GAGTGCGTGC GACAAATGGC CGACATCCTG
    AGCCAGGTGC GGGGCCCAGG GAACGCATCC CCCAACGCAC GGGCCTCGGT GACTCAGGAT
    GCAGACAGTG TGCTCCGGCC TCTCATGGAC TTCCTGGACG GCAACCTCAC ACTTTTTGCC
    ACTGTGTGTG AGAAGACGGT CCTGAAGCGG GTACTGAAGG AACTCTGGCG GGTGGTAATG
    AACACAATGG AAAGGATGAT TGTTCTGCCT CCACTTACTG ACCAGACGGG CACCCAGCTG
    ATCTTCACTG CTGCCAAGGA GCTGAGCCAA CTTTCCAAAC TCAAGGACCA CATGGTGCGA
    GAGGAAACAC GGAATCTGAC TCCAAAGCAG TGTGCCGTCC TTGACCTCGC CCTGGACACC
    ATCAAGCAAT ACTTTCACGC CGGAGGCAAC GGGCTGAAGA AAACCTTCCT GGAGAAGAGC
    CCAGATCTGC AGTCCCTGCG CTATGCCCTA TCTCTGTACA CACAGACCAC AGACACCCTC
    ATCAAGACCT TTGTGCGCTC ACAGACTGCC CAGGTGCATG ATGGGAAAGG TATTAGGTTT
    ACTGCTAATG AGGACATTCA GCCGGAAAAG GGGTCCGGTG TGGATGATCC TGTGGGAGAA
    GTCTCTATTC AGGTGGACTT GTTTACACAT CCTGGAACTG GGGAGCACAA GGTCACAGTG
    AAAGTGGTGG CTGCCAATGA CCTCAAGTGG CAGACAGCAG GTATGTTCCG GCCCTTTGTC
    GAAGTCACCA TGGTTGGCCC TCACCAAAGT GATAAAAAGA GGAAGTTCAC AACCAAGTCA
    AAAAGTAACA ACTGGGCTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA
    GAGGGGCCAG AAGCCTATGA GTTGCAGATA TGCGTGAAGG ATTACTGCTT TGCCCGGGAG
    GACCATGTGC TGGGGCTGGC TGTAATGCCT CTGAGGGATG TGGCAGCCAA GGGCAGCTGT
    GCTTGCTGGT GCCCCTTGGG CCGTAAGATC CACATGGATG AGACAGGCGT GACCATTCTC
    CGGATTCTGT CTCAGAGGAG CAATGACGAA GTGGCCCGGG AGTTTGTGAA GCTCAAATCA
    GAGTCTCGTT CTATGGAGGA GGGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328008.2
    CDS81..6050
    Translation

    Target ORF information:

    RefSeq Version XM_015472522.2
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472522.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GGGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGATAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA
    GTCAGCGCAG AAGTGATGTG GCAACTCTTT GCCCAAGACA TGAAATATGC ACTGGAGGAG
    CATGAGAAAG ACAGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG
    TGGCTCCACA ATGAATACGT GCAGGATCTG CCTGCCCTCC AGGGCCAGGT GCCCGAATAC
    CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG TGGCTGGACG AGAATGAGGA TGTGTCCTTG
    GAATTCCTGC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG
    CACGCGCTCT TCTCTTGCTC TGTGGTGGAC GTCTTCACAC AGCTCAATCA GAGCTTTGAG
    ATCATCCGGA AATTGGAATG CCCAGACCCC AACATCCTTG CCCACTACAT GAGGAGATTT
    TCTAAGACCA TCGGGAAGGT GCTCATGGAA TATGCAGACA TCTTATCAAA GAACTTCCCA
    GCTTACTGCA CAAAGGAGAA AATGCCTTGC ATCCTGATGA ACAACATGCA GCAGCTGAGG
    GTGCAGCTGG AGAAGATGTT TGAGGCCATG GGAGGCAAGG AGCTGGACCC TGAAACTGCA
    GACAGTCTGA AGGAGCTTCA GGTGAAACTG AATACAGTTT TGGATGAGCT CAGCATGGTG
    TTTGGAAACA GTTTCCAGGT GCGGATTGAT GAGTGCGTGC GACAAATGGC CGACATCCTG
    AGCCAGGTGC GGGGCCCAGG GAACGCATCC CCCAACGCAC GGGCCTCGGT GACTCAGGAT
    GCAGACAGTG TGCTCCGGCC TCTCATGGAC TTCCTGGACG GCAACCTCAC ACTTTTTGCC
    ACTGTGTGTG AGAAGACGGT CCTGAAGCGG GTACTGAAGG AACTCTGGCG GGTGGTAATG
    AACACAATGG AAAGGATGAT TGTTCTGCCT CCACTTACTG ACCAGACGGG CACCCAGCTG
    ATCTTCACTG CTGCCAAGGA GCTGAGCCAA CTTTCCAAAC TCAAGGACCA CATGGTGCGA
    GAGGAAACAC GGAATCTGAC TCCAAAGCAG TGTGCCGTCC TTGACCTCGC CCTGGACACC
    ATCAAGCAAT ACTTTCACGC CGGAGGCAAC GGGCTGAAGA AAACCTTCCT GGAGAAGAGC
    CCAGATCTGC AGTCCCTGCG CTATGCCCTA TCTCTGTACA CACAGACCAC AGACACCCTC
    ATCAAGACCT TTGTGCGCTC ACAGACTGCC CAGGTGCATG ATGGGAAAGG TATTAGGTTT
    ACTGCTAATG AGGACATTCA GCCGGAAAAG GGGTCCGGTG TGGATGATCC TGTGGGAGAA
    GTCTCTATTC AGGTGGACTT GTTTACACAT CCTGGAACTG GGGAGCACAA GGTCACAGTG
    AAAGTGGTGG CTGCCAATGA CCTCAAGTGG CAGACAGCAG GTATGTTCCG GCCCTTTGTC
    GAAGTCACCA TGGTTGGCCC TCACCAAAGT GATAAAAAGA GGAAGTTCAC AACCAAGTCA
    AAAAGTAACA ACTGGGCTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA
    GAGGGGCCAG AAGCCTATGA GTTGCAGATA TGCGTGAAGG ATTACTGCTT TGCCCGGGAG
    GACCATGTGC TGGGGCTGGC TGTAATGCCT CTGAGGGATG TGGCAGCCAA GGGCAGCTGT
    GCTTGCTGGT GCCCCTTGGG CCGTAAGATC CACATGGATG AGACAGGCGT GACCATTCTC
    CGGATTCTGT CTCAGAGGAG CAATGACGAA GTGGCCCGGG AGTTTGTGAA GCTCAAATCA
    GAGTCTCGTT CTATGGAGGA GGGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237126
    Clone ID Related Accession (Same CDS sequence) XM_024995782.1
    Accession Version XM_024995782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GGGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGATAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA
    GTCAGCGCAG AAGTGATGTG GCAACTCTTT GCCCAAGACA TGAAATATGC ACTGGAGGAG
    CATGAGAAAG ACAGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG
    TGGCTCCACA ATGAATACGT GCAGGATCTG CCTGCCCTCC AGGGCCAGGT GCCCGAATAC
    CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG TGGCTGGACG AGAATGAGGA TGTGTCCTTG
    GAATTCCTGC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG
    CACGCGCTCT TCTCTTGCTC TGTGGTGGAC GTCTTCACAC AGCTCAATCA GAGCTTTGAG
    ATCATCCGGA AATTGGAATG CCCAGACCCC AACATCCTTG CCCACTACAT GAGGAGATTT
    TCTAAGACCA TCGGGAAGGT GCTCATGGAA TATGCAGACA TCTTATCAAA GAACTTCCCA
    GCTTACTGCA CAAAGGAGAA AATGCCTTGC ATCCTGATGA ACAACATGCA GCAGCTGAGG
    GTGCAGCTGG AGAAGATGTT TGAGGCCATG GGAGGCAAGG AGCTGGACCC TGAAACTGCA
    GACAGTCTGA AGGAGCTTCA GGTGAAACTG AATACAGTTT TGGATGAGCT CAGCATGGTG
    TTTGGAAACA GTTTCCAGGT GCGGATTGAT GAGTGCGTGC GACAAATGGC CGACATCCTG
    AGCCAGGTGC GGGGCCCAGG GAACGCATCC CCCAACGCAC GGGCCTCGGT GACTCAGGAT
    GCAGACAGTG TGCTCCGGCC TCTCATGGAC TTCCTGGACG GCAACCTCAC ACTTTTTGCC
    ACTGTGTGTG AGAAGACGGT CCTGAAGCGG GTACTGAAGG AACTCTGGCG GGTGGTAATG
    AACACAATGG AAAGGATGAT TGTTCTGCCT CCACTTACTG ACCAGACGGG CACCCAGCTG
    ATCTTCACTG CTGCCAAGGA GCTGAGCCAA CTTTCCAAAC TCAAGGACCA CATGGTGCGA
    GAGGAAACAC GGAATCTGAC TCCAAAGCAG TGTGCCGTCC TTGACCTCGC CCTGGACACC
    ATCAAGCAAT ACTTTCACGC CGGAGGCAAC GGGCTGAAGA AAACCTTCCT GGAGAAGAGC
    CCAGATCTGC AGTCCCTGCG CTATGCCCTA TCTCTGTACA CACAGACCAC AGACACCCTC
    ATCAAGACCT TTGTGCGCTC ACAGACTGCC CAGGGGTCCG GTGTGGATGA TCCTGTGGGA
    GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA
    GTGAAAGTGG TGGCTGCCAA TGACCTCAAG TGGCAGACAG CAGGTATGTT CCGGCCCTTT
    GTCGAAGTCA CCATGGTTGG CCCTCACCAA AGTGATAAAA AGAGGAAGTT CACAACCAAG
    TCAAAAAGTA ACAACTGGGC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT
    GAAGAGGGGC CAGAAGCCTA TGAGTTGCAG ATATGCGTGA AGGATTACTG CTTTGCCCGG
    GAGGACCATG TGCTGGGGCT GGCTGTAATG CCTCTGAGGG ATGTGGCAGC CAAGGGCAGC
    TGTGCTTGCT GGTGCCCCTT GGGCCGTAAG ATCCACATGG ATGAGACAGG CGTGACCATT
    CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAAGTGGCCC GGGAGTTTGT GAAGCTCAAA
    TCAGAGTCTC GTTCTATGGA GGAGGGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851550.1
    CDS81..5993
    Translation

    Target ORF information:

    RefSeq Version XM_024995782.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GGGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGATAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA
    GTCAGCGCAG AAGTGATGTG GCAACTCTTT GCCCAAGACA TGAAATATGC ACTGGAGGAG
    CATGAGAAAG ACAGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG
    TGGCTCCACA ATGAATACGT GCAGGATCTG CCTGCCCTCC AGGGCCAGGT GCCCGAATAC
    CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG TGGCTGGACG AGAATGAGGA TGTGTCCTTG
    GAATTCCTGC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG
    CACGCGCTCT TCTCTTGCTC TGTGGTGGAC GTCTTCACAC AGCTCAATCA GAGCTTTGAG
    ATCATCCGGA AATTGGAATG CCCAGACCCC AACATCCTTG CCCACTACAT GAGGAGATTT
    TCTAAGACCA TCGGGAAGGT GCTCATGGAA TATGCAGACA TCTTATCAAA GAACTTCCCA
    GCTTACTGCA CAAAGGAGAA AATGCCTTGC ATCCTGATGA ACAACATGCA GCAGCTGAGG
    GTGCAGCTGG AGAAGATGTT TGAGGCCATG GGAGGCAAGG AGCTGGACCC TGAAACTGCA
    GACAGTCTGA AGGAGCTTCA GGTGAAACTG AATACAGTTT TGGATGAGCT CAGCATGGTG
    TTTGGAAACA GTTTCCAGGT GCGGATTGAT GAGTGCGTGC GACAAATGGC CGACATCCTG
    AGCCAGGTGC GGGGCCCAGG GAACGCATCC CCCAACGCAC GGGCCTCGGT GACTCAGGAT
    GCAGACAGTG TGCTCCGGCC TCTCATGGAC TTCCTGGACG GCAACCTCAC ACTTTTTGCC
    ACTGTGTGTG AGAAGACGGT CCTGAAGCGG GTACTGAAGG AACTCTGGCG GGTGGTAATG
    AACACAATGG AAAGGATGAT TGTTCTGCCT CCACTTACTG ACCAGACGGG CACCCAGCTG
    ATCTTCACTG CTGCCAAGGA GCTGAGCCAA CTTTCCAAAC TCAAGGACCA CATGGTGCGA
    GAGGAAACAC GGAATCTGAC TCCAAAGCAG TGTGCCGTCC TTGACCTCGC CCTGGACACC
    ATCAAGCAAT ACTTTCACGC CGGAGGCAAC GGGCTGAAGA AAACCTTCCT GGAGAAGAGC
    CCAGATCTGC AGTCCCTGCG CTATGCCCTA TCTCTGTACA CACAGACCAC AGACACCCTC
    ATCAAGACCT TTGTGCGCTC ACAGACTGCC CAGGGGTCCG GTGTGGATGA TCCTGTGGGA
    GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA
    GTGAAAGTGG TGGCTGCCAA TGACCTCAAG TGGCAGACAG CAGGTATGTT CCGGCCCTTT
    GTCGAAGTCA CCATGGTTGG CCCTCACCAA AGTGATAAAA AGAGGAAGTT CACAACCAAG
    TCAAAAAGTA ACAACTGGGC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT
    GAAGAGGGGC CAGAAGCCTA TGAGTTGCAG ATATGCGTGA AGGATTACTG CTTTGCCCGG
    GAGGACCATG TGCTGGGGCT GGCTGTAATG CCTCTGAGGG ATGTGGCAGC CAAGGGCAGC
    TGTGCTTGCT GGTGCCCCTT GGGCCGTAAG ATCCACATGG ATGAGACAGG CGTGACCATT
    CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAAGTGGCCC GGGAGTTTGT GAAGCTCAAA
    TCAGAGTCTC GTTCTATGGA GGAGGGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237127
    Clone ID Related Accession (Same CDS sequence) XM_024995783.1
    Accession Version XM_024995783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGAAG GAAAGCGAGG AGCCCTTCAG GTTGTGCTCT
    GGCCCTTTGG GGCCCCTTTG TTGTTGGTTC TCTCCCCTCC CTCCTGAGTA CAATATTCCA
    GATGGTGGTG ATCCCTGTAT GCCCCAGTGG CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT
    GGCATTGACA GCATGCCGGA TCTACGCAGA AAGAAGCCAC TGCCGCTTGT CAGCGACCTG
    TCACTGGTCC AGTCCCGGAA AGCAGGCATT ACTTCTGCAA TGGCTACACG CACCTCTCTC
    AAAGATGAAG AGCTGAAATC ACATGTGTAT AAGAAAACCC TGCAGGCCTT AATCTATCCC
    ATCTCGTGCA CCACACCCCA CAACTTTGAG GTGTGGTCAG CCACCACCCC CACCTACTGC
    TATGAGTGTG AAGGCCTGCT CTGGGGCATT GCCCGGCAGG GCATGCGCTG CAGCGAGTGT
    GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTACTCAATG CTGACTGCCT GCAGCGGGCT
    GCAGAAAAGA GCTCTAAACA TGGAGCGGAG GACCGGACCC AGAACATCAT CATGGCCATG
    AAGGACCGCA TGAAGATCCG AGAGCGGAAT AAGCCAGAGA TCTTTGAAGT TATCCGGGAT
    GTCTTCAATG TGAGCAAAGT CGCCCATGTG CAACAAATGA AAACAGTGAA GCAGAGTGTG
    TTGGATGGCA CCTCCAAATG GTCAGCCAAA ATCACCATCA CTGTGGTGTG TGCCCAGGGC
    CTACAAGCCA AGGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTGGGCAAA
    ACCAAGAAGC GTACCAAGAC CATTTTTGGA AACCTGAATC CTGTTTGGGA GGAGAAGTTC
    CATTTTGAGT GCCACAACTC TTCTGACCGC ATTAAGGTAC GTGTGTGGGA TGAGGATGAT
    GACATCAAGT CACGAGTAAA GCAGCGGCTA AAGCGAGAGT CTGATGATTT CCTCGGCCAA
    ACCATCATTG AGGTTCGAAC ACTGAGTGGC GAAATGGACG TCTGGTACAA CTTGGAGAAG
    AGGACAGACA AGTCAGCCGT CTCGGGGGCC ATCCGACTGC AAATCAGCGT GGAGATCAAG
    GGAGAAGAGA AGGTAGCCCC ATATCATGTG CAGTACACGT GTCTCCATGA GAACCTCTTC
    CATTACCTCA CGGACATTCA GGGCAGCGGA GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT
    GATGCATGGA AGGTGTACTT TGATGAGACT GCCCAGGAAA TTGTGGATGA ATTTGCCATG
    CGCTATGGCA TTGAGTCCAT ATATCAGGCC ATGACGCATT TTGCATGTTT GTCATCCAAG
    TACATGTGTC CTGGTGTGCC AGCAGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAC
    TATGCCCACA CAACTGCCTC CACCAATGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC
    TTTGGGAAAG AGAGATTTGT GAAACTTCTG GACCAGCTGC ACAACTCACT GAGGATTGAC
    CTCTCTACCT ACAGGAATAA TTTCCCTGCT GGGAGCCCTG AGCGACTTCA GGACTTAAAG
    TCCACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCCGAA TGAAGGTGCA AGAACTTCAG
    AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA GATTGTGTGA AGGCCTGTTT GAACTCAACA
    TATGAGTATA TCTTCAACAA CTGCCATGAC TTGTACAGCC GCCAGTACCA GCTGAATCAG
    GAGCTGCCTC CAGAAGAACA GGGGCCCAGC ATTAGGAACC TGGATTTCTG GCCCAAACTC
    ATCACACTCA TTGTGTCAAT CATAGAGGAA GATAAGAATT CCTACACCCT CGTTCTGAAC
    CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA GTCAGCGCAG AAGTGATGTG GCAACTCTTT
    GCCCAAGACA TGAAATATGC ACTGGAGGAG CATGAGAAAG ACAGGCTGTG TAAGAGTGCT
    GACTACATGA ACCTGCACTT CAAGGTGAAG TGGCTCCACA ATGAATACGT GCAGGATCTG
    CCTGCCCTCC AGGGCCAGGT GCCCGAATAC CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG
    TGGCTGGACG AGAATGAGGA TGTGTCCTTG GAATTCCTGC GTGGGGCCTT GGAACGAGAT
    AAGAAGGATG GGTTCCAGCA GACATCAGAG CACGCGCTCT TCTCTTGCTC TGTGGTGGAC
    GTCTTCACAC AGCTCAATCA GAGCTTTGAG ATCATCCGGA AATTGGAATG CCCAGACCCC
    AACATCCTTG CCCACTACAT GAGGAGATTT TCTAAGACCA TCGGGAAGGT GCTCATGGAA
    TATGCAGACA TCTTATCAAA GAACTTCCCA GCTTACTGCA CAAAGGAGAA AATGCCTTGC
    ATCCTGATGA ACAACATGCA GCAGCTGAGG GTGCAGCTGG AGAAGATGTT TGAGGCCATG
    GGAGGCAAGG AGCTGGACCC TGAAACTGCA GACAGTCTGA AGGAGCTTCA GGTGAAACTG
    AATACAGTTT TGGATGAGCT CAGCATGGTG TTTGGAAACA GTTTCCAGGT GCGGATTGAT
    GAGTGCGTGC GACAAATGGC CGACATCCTG AGCCAGGTGC GGGGCCCAGG GAACGCATCC
    CCCAACGCAC GGGCCTCGGT GACTCAGGAT GCAGACAGTG TGCTCCGGCC TCTCATGGAC
    TTCCTGGACG GCAACCTCAC ACTTTTTGCC ACTGTGTGTG AGAAGACGGT CCTGAAGCGG
    GTACTGAAGG AACTCTGGCG GGTGGTAATG AACACAATGG AAAGGATGAT TGTTCTGCCT
    CCACTTACTG ACCAGACGGG CACCCAGCTG ATCTTCACTG CTGCCAAGGA GCTGAGCCAA
    CTTTCCAAAC TCAAGGACCA CATGGTGCGA GAGGAAACAC GGAATCTGAC TCCAAAGCAG
    TGTGCCGTCC TTGACCTCGC CCTGGACACC ATCAAGCAAT ACTTTCACGC CGGAGGCAAC
    GGGCTGAAGA AAACCTTCCT GGAGAAGAGC CCAGATCTGC AGTCCCTGCG CTATGCCCTA
    TCTCTGTACA CACAGACCAC AGACACCCTC ATCAAGACCT TTGTGCGCTC ACAGACTGCC
    CAGGTGCATG ATGGGAAAGG TATTAGGTTT ACTGCTAATG AGGACATTCA GCCGGAAAAG
    GGGTCCGGTG TGGATGATCC TGTGGGAGAA GTCTCTATTC AGGTGGACTT GTTTACACAT
    CCTGGAACTG GGGAGCACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA CCTCAAGTGG
    CAGACAGCAG GTATGTTCCG GCCCTTTGTC GAAGTCACCA TGGTTGGCCC TCACCAAAGT
    GATAAAAAGA GGAAGTTCAC AACCAAGTCA AAAAGTAACA ACTGGGCTCC CAAGTACAAC
    GAGACGTTTC ACTTCCTCCT GGGAAATGAA GAGGGGCCAG AAGCCTATGA GTTGCAGATA
    TGCGTGAAGG ATTACTGCTT TGCCCGGGAG GACCATGTGC TGGGGCTGGC TGTAATGCCT
    CTGAGGGATG TGGCAGCCAA GGGCAGCTGT GCTTGCTGGT GCCCCTTGGG CCGTAAGATC
    CACATGGATG AGACAGGCGT GACCATTCTC CGGATTCTGT CTCAGAGGAG CAATGACGAA
    GTGGCCCGGG AGTTTGTGAA GCTCAAATCA GAGTCTCGTT CTATGGAGGA GGGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851551.1
    CDS331..5250
    Translation

    Target ORF information:

    RefSeq Version XM_024995783.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGAAG GAAAGCGAGG AGCCCTTCAG GTTGTGCTCT
    GGCCCTTTGG GGCCCCTTTG TTGTTGGTTC TCTCCCCTCC CTCCTGAGTA CAATATTCCA
    GATGGTGGTG ATCCCTGTAT GCCCCAGTGG CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT
    GGCATTGACA GCATGCCGGA TCTACGCAGA AAGAAGCCAC TGCCGCTTGT CAGCGACCTG
    TCACTGGTCC AGTCCCGGAA AGCAGGCATT ACTTCTGCAA TGGCTACACG CACCTCTCTC
    AAAGATGAAG AGCTGAAATC ACATGTGTAT AAGAAAACCC TGCAGGCCTT AATCTATCCC
    ATCTCGTGCA CCACACCCCA CAACTTTGAG GTGTGGTCAG CCACCACCCC CACCTACTGC
    TATGAGTGTG AAGGCCTGCT CTGGGGCATT GCCCGGCAGG GCATGCGCTG CAGCGAGTGT
    GGAGTCAAGT GCCATGAGAA GTGCCAGGAC CTACTCAATG CTGACTGCCT GCAGCGGGCT
    GCAGAAAAGA GCTCTAAACA TGGAGCGGAG GACCGGACCC AGAACATCAT CATGGCCATG
    AAGGACCGCA TGAAGATCCG AGAGCGGAAT AAGCCAGAGA TCTTTGAAGT TATCCGGGAT
    GTCTTCAATG TGAGCAAAGT CGCCCATGTG CAACAAATGA AAACAGTGAA GCAGAGTGTG
    TTGGATGGCA CCTCCAAATG GTCAGCCAAA ATCACCATCA CTGTGGTGTG TGCCCAGGGC
    CTACAAGCCA AGGACAAGAC AGGATCCAGT GACCCTTATG TGACTGTGCA AGTGGGCAAA
    ACCAAGAAGC GTACCAAGAC CATTTTTGGA AACCTGAATC CTGTTTGGGA GGAGAAGTTC
    CATTTTGAGT GCCACAACTC TTCTGACCGC ATTAAGGTAC GTGTGTGGGA TGAGGATGAT
    GACATCAAGT CACGAGTAAA GCAGCGGCTA AAGCGAGAGT CTGATGATTT CCTCGGCCAA
    ACCATCATTG AGGTTCGAAC ACTGAGTGGC GAAATGGACG TCTGGTACAA CTTGGAGAAG
    AGGACAGACA AGTCAGCCGT CTCGGGGGCC ATCCGACTGC AAATCAGCGT GGAGATCAAG
    GGAGAAGAGA AGGTAGCCCC ATATCATGTG CAGTACACGT GTCTCCATGA GAACCTCTTC
    CATTACCTCA CGGACATTCA GGGCAGCGGA GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT
    GATGCATGGA AGGTGTACTT TGATGAGACT GCCCAGGAAA TTGTGGATGA ATTTGCCATG
    CGCTATGGCA TTGAGTCCAT ATATCAGGCC ATGACGCATT TTGCATGTTT GTCATCCAAG
    TACATGTGTC CTGGTGTGCC AGCAGTGATG AGCACCTTAC TGGCCAACAT CAATGCCTAC
    TATGCCCACA CAACTGCCTC CACCAATGTC TCTGCATCTG ATCGTTTTGC AGCCTCCAAC
    TTTGGGAAAG AGAGATTTGT GAAACTTCTG GACCAGCTGC ACAACTCACT GAGGATTGAC
    CTCTCTACCT ACAGGAATAA TTTCCCTGCT GGGAGCCCTG AGCGACTTCA GGACTTAAAG
    TCCACAGTGG ATTTGCTGAC CAGCATTACT TTCTTCCGAA TGAAGGTGCA AGAACTTCAG
    AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA GATTGTGTGA AGGCCTGTTT GAACTCAACA
    TATGAGTATA TCTTCAACAA CTGCCATGAC TTGTACAGCC GCCAGTACCA GCTGAATCAG
    GAGCTGCCTC CAGAAGAACA GGGGCCCAGC ATTAGGAACC TGGATTTCTG GCCCAAACTC
    ATCACACTCA TTGTGTCAAT CATAGAGGAA GATAAGAATT CCTACACCCT CGTTCTGAAC
    CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA GTCAGCGCAG AAGTGATGTG GCAACTCTTT
    GCCCAAGACA TGAAATATGC ACTGGAGGAG CATGAGAAAG ACAGGCTGTG TAAGAGTGCT
    GACTACATGA ACCTGCACTT CAAGGTGAAG TGGCTCCACA ATGAATACGT GCAGGATCTG
    CCTGCCCTCC AGGGCCAGGT GCCCGAATAC CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG
    TGGCTGGACG AGAATGAGGA TGTGTCCTTG GAATTCCTGC GTGGGGCCTT GGAACGAGAT
    AAGAAGGATG GGTTCCAGCA GACATCAGAG CACGCGCTCT TCTCTTGCTC TGTGGTGGAC
    GTCTTCACAC AGCTCAATCA GAGCTTTGAG ATCATCCGGA AATTGGAATG CCCAGACCCC
    AACATCCTTG CCCACTACAT GAGGAGATTT TCTAAGACCA TCGGGAAGGT GCTCATGGAA
    TATGCAGACA TCTTATCAAA GAACTTCCCA GCTTACTGCA CAAAGGAGAA AATGCCTTGC
    ATCCTGATGA ACAACATGCA GCAGCTGAGG GTGCAGCTGG AGAAGATGTT TGAGGCCATG
    GGAGGCAAGG AGCTGGACCC TGAAACTGCA GACAGTCTGA AGGAGCTTCA GGTGAAACTG
    AATACAGTTT TGGATGAGCT CAGCATGGTG TTTGGAAACA GTTTCCAGGT GCGGATTGAT
    GAGTGCGTGC GACAAATGGC CGACATCCTG AGCCAGGTGC GGGGCCCAGG GAACGCATCC
    CCCAACGCAC GGGCCTCGGT GACTCAGGAT GCAGACAGTG TGCTCCGGCC TCTCATGGAC
    TTCCTGGACG GCAACCTCAC ACTTTTTGCC ACTGTGTGTG AGAAGACGGT CCTGAAGCGG
    GTACTGAAGG AACTCTGGCG GGTGGTAATG AACACAATGG AAAGGATGAT TGTTCTGCCT
    CCACTTACTG ACCAGACGGG CACCCAGCTG ATCTTCACTG CTGCCAAGGA GCTGAGCCAA
    CTTTCCAAAC TCAAGGACCA CATGGTGCGA GAGGAAACAC GGAATCTGAC TCCAAAGCAG
    TGTGCCGTCC TTGACCTCGC CCTGGACACC ATCAAGCAAT ACTTTCACGC CGGAGGCAAC
    GGGCTGAAGA AAACCTTCCT GGAGAAGAGC CCAGATCTGC AGTCCCTGCG CTATGCCCTA
    TCTCTGTACA CACAGACCAC AGACACCCTC ATCAAGACCT TTGTGCGCTC ACAGACTGCC
    CAGGTGCATG ATGGGAAAGG TATTAGGTTT ACTGCTAATG AGGACATTCA GCCGGAAAAG
    GGGTCCGGTG TGGATGATCC TGTGGGAGAA GTCTCTATTC AGGTGGACTT GTTTACACAT
    CCTGGAACTG GGGAGCACAA GGTCACAGTG AAAGTGGTGG CTGCCAATGA CCTCAAGTGG
    CAGACAGCAG GTATGTTCCG GCCCTTTGTC GAAGTCACCA TGGTTGGCCC TCACCAAAGT
    GATAAAAAGA GGAAGTTCAC AACCAAGTCA AAAAGTAACA ACTGGGCTCC CAAGTACAAC
    GAGACGTTTC ACTTCCTCCT GGGAAATGAA GAGGGGCCAG AAGCCTATGA GTTGCAGATA
    TGCGTGAAGG ATTACTGCTT TGCCCGGGAG GACCATGTGC TGGGGCTGGC TGTAATGCCT
    CTGAGGGATG TGGCAGCCAA GGGCAGCTGT GCTTGCTGGT GCCCCTTGGG CCGTAAGATC
    CACATGGATG AGACAGGCGT GACCATTCTC CGGATTCTGT CTCAGAGGAG CAATGACGAA
    GTGGCCCGGG AGTTTGTGAA GCTCAAATCA GAGTCTCGTT CTATGGAGGA GGGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237128
    Clone ID Related Accession (Same CDS sequence) XM_005210089.3
    Accession Version XM_005210089.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGTGC ATGATGGGAA AGGTATTAGG
    TTTACTGCTA ATGAGGACAT TCAGCCGGAA AAGGGGTCCG GTGTGGATGA TCCTGTGGGA
    GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA
    GTGAAAGTGG TGGCTGCCAA TGACCTCAAG TGGCAGACAG CAGGTATGTT CCGGCCCTTT
    GTCGAAGTCA CCATGGTTGG CCCTCACCAA AGTGATAAAA AGAGGAAGTT CACAACCAAG
    TCAAAAAGTA ACAACTGGGC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT
    GAAGAGGGGC CAGAAGCCTA TGAGTTGCAG ATATGCGTGA AGGATTACTG CTTTGCCCGG
    GAGGACCATG TGCTGGGGCT GGCTGTAATG CCTCTGAGGG ATGTGGCAGC CAAGGGCAGC
    TGTGCTTGCT GGTGCCCCTT GGGCCGTAAG ATCCACATGG ATGAGACAGG CGTGACCATT
    CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAAGTGGCCC GGGAGTTTGT GAAGCTCAAA
    TCAGAGTCTC GTTCTATGGA GGAGGGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210146.2
    CDS331..5163
    Translation

    Target ORF information:

    RefSeq Version XM_005210089.3
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (UNC13B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210089.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGTGC ATGATGGGAA AGGTATTAGG
    TTTACTGCTA ATGAGGACAT TCAGCCGGAA AAGGGGTCCG GTGTGGATGA TCCTGTGGGA
    GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA
    GTGAAAGTGG TGGCTGCCAA TGACCTCAAG TGGCAGACAG CAGGTATGTT CCGGCCCTTT
    GTCGAAGTCA CCATGGTTGG CCCTCACCAA AGTGATAAAA AGAGGAAGTT CACAACCAAG
    TCAAAAAGTA ACAACTGGGC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT
    GAAGAGGGGC CAGAAGCCTA TGAGTTGCAG ATATGCGTGA AGGATTACTG CTTTGCCCGG
    GAGGACCATG TGCTGGGGCT GGCTGTAATG CCTCTGAGGG ATGTGGCAGC CAAGGGCAGC
    TGTGCTTGCT GGTGCCCCTT GGGCCGTAAG ATCCACATGG ATGAGACAGG CGTGACCATT
    CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAAGTGGCCC GGGAGTTTGT GAAGCTCAAA
    TCAGAGTCTC GTTCTATGGA GGAGGGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91271
    Clone ID Related Accession (Same CDS sequence) XM_015472521.1
    Accession Version XM_015472521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5976bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GAGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGACAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG GCTATGTCAC TGGTCCAGTC CCGGAAAGCA
    GGCATTACTT CTGCAATGGC TACACGCACC TCTCTCAAAG ATGAAGAGCT GAAATCACAT
    GTGTATAAGA AAACCCTGCA GGCCTTAATC TATCCCATCT CGTGCACCAC ACCCCACAAC
    TTTGAGGTGT GGTCAGCCAC CACCCCCACC TACTGCTATG AGTGTGAAGG CCTGCTCTGG
    GGCATTGCCC GGCAGGGCAT GCGCTGCAGC GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC
    CAGGACCTAC TCAATGCTGA CTGCCTGCAG CGGGCTGCAG AAAAGAGCTC TAAACATGGA
    GCGGAGGACC GGACCCAGAA CATCATCATG GCCATGAAGG ACCGCATGAA GATCCGAGAG
    CGGAATAAGC CAGAGATCTT TGAAGTTATC CGGGATGTCT TCAATGTGAG CAAAGTCGCC
    CATGTGCAAC AAATGAAAAC AGTGAAGCAG AGTGTGTTGG ATGGCACCTC CAAATGGTCA
    GCCAAAATCA CCATCACTGT GGTGTGTGCC CAGGGCCTAC AAGCCAAGGA CAAGACAGGA
    TCCAGTGACC CTTATGTGAC TGTGCAAGTG GGCAAAACCA AGAAGCGTAC CAAGACCATT
    TTTGGAAACC TGAATCCTGT TTGGGAGGAG AAGTTCCATT TTGAGTGCCA CAACTCTTCT
    GACCGCATTA AGGTACGTGT GTGGGATGAG GATGATGACA TCAAGTCACG AGTAAAGCAG
    CGGCTAAAGC GAGAGTCTGA TGATTTCCTC GGCCAAACCA TCATTGAGGT TCGAACACTG
    AGTGGCGAAA TGGACGTCTG GTACAACTTG GAGAAGAGGA CAGACAAGTC AGCCGTCTCG
    GGGGCCATCC GACTGCAAAT CAGCGTGGAG ATCAAGGGAG AAGAGAAGGT AGCCCCATAT
    CATGTGCAGT ACACGTGTCT CCATGAGAAC CTCTTCCATT ACCTCACGGA CATTCAGGGC
    AGCGGAGGAG TCCAGATCCC TGAGGCTCGA GGAGATGATG CATGGAAGGT GTACTTTGAT
    GAGACTGCCC AGGAAATTGT GGATGAATTT GCCATGCGCT ATGGCATTGA GTCCATATAT
    CAGGCCATGA CGCATTTTGC ATGTTTGTCA TCCAAGTACA TGTGTCCTGG TGTGCCAGCA
    GTGATGAGCA CCTTACTGGC CAACATCAAT GCCTACTATG CCCACACAAC TGCCTCCACC
    AATGTCTCTG CATCTGATCG TTTTGCAGCC TCCAACTTTG GGAAAGAGAG ATTTGTGAAA
    CTTCTGGACC AGCTGCACAA CTCACTGAGG ATTGACCTCT CTACCTACAG GAATAATTTC
    CCTGCTGGGA GCCCTGAGCG ACTTCAGGAC TTAAAGTCCA CAGTGGATTT GCTGACCAGC
    ATTACTTTCT TCCGAATGAA GGTGCAAGAA CTTCAGAGCC CTCCAAGAGC CAGCCAGGTA
    GTAAAAGATT GTGTGAAGGC CTGTTTGAAC TCAACATATG AGTATATCTT CAACAACTGC
    CATGACTTGT ACAGCCGCCA GTACCAGCTG AATCAGGAGC TGCCTCCAGA AGAACAGGGG
    CCCAGCATTA GGAACCTGGA TTTCTGGCCC AAACTCATCA CACTCATTGT GTCAATCATA
    GAGGAAGATA AGAATTCCTA CACCCTCGTT CTGAACCAGT TTCCCCAGGA GTTGAATGTG
    GGAAAAGTCA GCGCAGAAGT GATGTGGCAA CTCTTTGCCC AAGACATGAA ATATGCACTG
    GAGGAGCATG AGAAAGACAG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG
    GTGAAGTGGC TCCACAATGA ATACGTGCAG GATCTGCCTG CCCTCCAGGG CCAGGTGCCC
    GAATACCCAG CGTGGTTTGA GCAGTTTGTC CTGCAGTGGC TGGACGAGAA TGAGGATGTG
    TCCTTGGAAT TCCTGCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA
    TCAGAGCACG CGCTCTTCTC TTGCTCTGTG GTGGACGTCT TCACACAGCT CAATCAGAGC
    TTTGAGATCA TCCGGAAATT GGAATGCCCA GACCCCAACA TCCTTGCCCA CTACATGAGG
    AGATTTTCTA AGACCATCGG GAAGGTGCTC ATGGAATATG CAGACATCTT ATCAAAGAAC
    TTCCCAGCTT ACTGCACAAA GGAGAAAATG CCTTGCATCC TGATGAACAA CATGCAGCAG
    CTGAGGGTGC AGCTGGAGAA GATGTTTGAG GCCATGGGAG GCAAGGAGCT GGACCCTGAA
    ACTGCAGACA GTCTGAAGGA GCTTCAGGTG AAACTGAATA CAGTTTTGGA TGAGCTCAGC
    ATGGTGTTTG GAAACAGTTT CCAGGTGCGG ATTGATGAGT GCGTGCGACA AATGGCCGAC
    ATCCTGAGCC AGGTGCGGGG CCCAGGGAAC GCATCCCCCA ACGCACGGGC CTCGGTGACT
    CAGGATGCAG ACAGTGTGCT CCGGCCTCTC ATGGACTTCC TGGACGGCAA CCTCACACTT
    TTTGCCACTG TGTGTGAGAA GACGGTCCTG AAGCGGGTAC TGAAGGAACT CTGGCGGGTG
    GTAATGAACA CAATGGAAAG GATGATTGTT CTGCCTCCAC TTACTGACCA GACGGGCACC
    CAGCTGATCT TCACTGCTGC CAAGGAGCTG AGCCAACTTT CCAAACTCAA GGACCACATG
    GTGCGAGAGG AAACACGGAA TCTGACTCCA AAGCAGTGTG CCGTCCTTGA CCTCGCCCTG
    GACACCATCA AGCAATACTT TCACGCCGGA GGCAACGGGC TGAAGAAAAC CTTCCTGGAG
    AAGAGCCCAG ATCTGCAGTC CCTGCGCTAT GCCCTATCTC TGTACACACA GACCACAGAC
    ACCCTCATCA AGACCTTTGT GCGCTCACAG ACTGCCCAGG TGCATGATGG GAAAGGTATT
    AGGTTTACTG CTAATGAGGA CATTCAGCCG GAAAAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC GTGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328007.1
    CDS378..6353
    Translation

    Target ORF information:

    RefSeq Version XM_015472521.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GAGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGACAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG GCTATGTCAC TGGTCCAGTC CCGGAAAGCA
    GGCATTACTT CTGCAATGGC TACACGCACC TCTCTCAAAG ATGAAGAGCT GAAATCACAT
    GTGTATAAGA AAACCCTGCA GGCCTTAATC TATCCCATCT CGTGCACCAC ACCCCACAAC
    TTTGAGGTGT GGTCAGCCAC CACCCCCACC TACTGCTATG AGTGTGAAGG CCTGCTCTGG
    GGCATTGCCC GGCAGGGCAT GCGCTGCAGC GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC
    CAGGACCTAC TCAATGCTGA CTGCCTGCAG CGGGCTGCAG AAAAGAGCTC TAAACATGGA
    GCGGAGGACC GGACCCAGAA CATCATCATG GCCATGAAGG ACCGCATGAA GATCCGAGAG
    CGGAATAAGC CAGAGATCTT TGAAGTTATC CGGGATGTCT TCAATGTGAG CAAAGTCGCC
    CATGTGCAAC AAATGAAAAC AGTGAAGCAG AGTGTGTTGG ATGGCACCTC CAAATGGTCA
    GCCAAAATCA CCATCACTGT GGTGTGTGCC CAGGGCCTAC AAGCCAAGGA CAAGACAGGA
    TCCAGTGACC CTTATGTGAC TGTGCAAGTG GGCAAAACCA AGAAGCGTAC CAAGACCATT
    TTTGGAAACC TGAATCCTGT TTGGGAGGAG AAGTTCCATT TTGAGTGCCA CAACTCTTCT
    GACCGCATTA AGGTACGTGT GTGGGATGAG GATGATGACA TCAAGTCACG AGTAAAGCAG
    CGGCTAAAGC GAGAGTCTGA TGATTTCCTC GGCCAAACCA TCATTGAGGT TCGAACACTG
    AGTGGCGAAA TGGACGTCTG GTACAACTTG GAGAAGAGGA CAGACAAGTC AGCCGTCTCG
    GGGGCCATCC GACTGCAAAT CAGCGTGGAG ATCAAGGGAG AAGAGAAGGT AGCCCCATAT
    CATGTGCAGT ACACGTGTCT CCATGAGAAC CTCTTCCATT ACCTCACGGA CATTCAGGGC
    AGCGGAGGAG TCCAGATCCC TGAGGCTCGA GGAGATGATG CATGGAAGGT GTACTTTGAT
    GAGACTGCCC AGGAAATTGT GGATGAATTT GCCATGCGCT ATGGCATTGA GTCCATATAT
    CAGGCCATGA CGCATTTTGC ATGTTTGTCA TCCAAGTACA TGTGTCCTGG TGTGCCAGCA
    GTGATGAGCA CCTTACTGGC CAACATCAAT GCCTACTATG CCCACACAAC TGCCTCCACC
    AATGTCTCTG CATCTGATCG TTTTGCAGCC TCCAACTTTG GGAAAGAGAG ATTTGTGAAA
    CTTCTGGACC AGCTGCACAA CTCACTGAGG ATTGACCTCT CTACCTACAG GAATAATTTC
    CCTGCTGGGA GCCCTGAGCG ACTTCAGGAC TTAAAGTCCA CAGTGGATTT GCTGACCAGC
    ATTACTTTCT TCCGAATGAA GGTGCAAGAA CTTCAGAGCC CTCCAAGAGC CAGCCAGGTA
    GTAAAAGATT GTGTGAAGGC CTGTTTGAAC TCAACATATG AGTATATCTT CAACAACTGC
    CATGACTTGT ACAGCCGCCA GTACCAGCTG AATCAGGAGC TGCCTCCAGA AGAACAGGGG
    CCCAGCATTA GGAACCTGGA TTTCTGGCCC AAACTCATCA CACTCATTGT GTCAATCATA
    GAGGAAGATA AGAATTCCTA CACCCTCGTT CTGAACCAGT TTCCCCAGGA GTTGAATGTG
    GGAAAAGTCA GCGCAGAAGT GATGTGGCAA CTCTTTGCCC AAGACATGAA ATATGCACTG
    GAGGAGCATG AGAAAGACAG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG
    GTGAAGTGGC TCCACAATGA ATACGTGCAG GATCTGCCTG CCCTCCAGGG CCAGGTGCCC
    GAATACCCAG CGTGGTTTGA GCAGTTTGTC CTGCAGTGGC TGGACGAGAA TGAGGATGTG
    TCCTTGGAAT TCCTGCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA
    TCAGAGCACG CGCTCTTCTC TTGCTCTGTG GTGGACGTCT TCACACAGCT CAATCAGAGC
    TTTGAGATCA TCCGGAAATT GGAATGCCCA GACCCCAACA TCCTTGCCCA CTACATGAGG
    AGATTTTCTA AGACCATCGG GAAGGTGCTC ATGGAATATG CAGACATCTT ATCAAAGAAC
    TTCCCAGCTT ACTGCACAAA GGAGAAAATG CCTTGCATCC TGATGAACAA CATGCAGCAG
    CTGAGGGTGC AGCTGGAGAA GATGTTTGAG GCCATGGGAG GCAAGGAGCT GGACCCTGAA
    ACTGCAGACA GTCTGAAGGA GCTTCAGGTG AAACTGAATA CAGTTTTGGA TGAGCTCAGC
    ATGGTGTTTG GAAACAGTTT CCAGGTGCGG ATTGATGAGT GCGTGCGACA AATGGCCGAC
    ATCCTGAGCC AGGTGCGGGG CCCAGGGAAC GCATCCCCCA ACGCACGGGC CTCGGTGACT
    CAGGATGCAG ACAGTGTGCT CCGGCCTCTC ATGGACTTCC TGGACGGCAA CCTCACACTT
    TTTGCCACTG TGTGTGAGAA GACGGTCCTG AAGCGGGTAC TGAAGGAACT CTGGCGGGTG
    GTAATGAACA CAATGGAAAG GATGATTGTT CTGCCTCCAC TTACTGACCA GACGGGCACC
    CAGCTGATCT TCACTGCTGC CAAGGAGCTG AGCCAACTTT CCAAACTCAA GGACCACATG
    GTGCGAGAGG AAACACGGAA TCTGACTCCA AAGCAGTGTG CCGTCCTTGA CCTCGCCCTG
    GACACCATCA AGCAATACTT TCACGCCGGA GGCAACGGGC TGAAGAAAAC CTTCCTGGAG
    AAGAGCCCAG ATCTGCAGTC CCTGCGCTAT GCCCTATCTC TGTACACACA GACCACAGAC
    ACCCTCATCA AGACCTTTGT GCGCTCACAG ACTGCCCAGG TGCATGATGG GAAAGGTATT
    AGGTTTACTG CTAATGAGGA CATTCAGCCG GAAAAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC GTGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91272
    Clone ID Related Accession (Same CDS sequence) XM_015472523.1
    Accession Version XM_015472523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GAGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGACAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG GCTATGTCAC TGGTCCAGTC CCGGAAAGCA
    GGCATTACTT CTGCAATGGC TACACGCACC TCTCTCAAAG ATGAAGAGCT GAAATCACAT
    GTGTATAAGA AAACCCTGCA GGCCTTAATC TATCCCATCT CGTGCACCAC ACCCCACAAC
    TTTGAGGTGT GGTCAGCCAC CACCCCCACC TACTGCTATG AGTGTGAAGG CCTGCTCTGG
    GGCATTGCCC GGCAGGGCAT GCGCTGCAGC GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC
    CAGGACCTAC TCAATGCTGA CTGCCTGCAG CGGGCTGCAG AAAAGAGCTC TAAACATGGA
    GCGGAGGACC GGACCCAGAA CATCATCATG GCCATGAAGG ACCGCATGAA GATCCGAGAG
    CGGAATAAGC CAGAGATCTT TGAAGTTATC CGGGATGTCT TCAATGTGAG CAAAGTCGCC
    CATGTGCAAC AAATGAAAAC AGTGAAGCAG AGTGTGTTGG ATGGCACCTC CAAATGGTCA
    GCCAAAATCA CCATCACTGT GGTGTGTGCC CAGGGCCTAC AAGCCAAGGA CAAGACAGGA
    TCCAGTGACC CTTATGTGAC TGTGCAAGTG GGCAAAACCA AGAAGCGTAC CAAGACCATT
    TTTGGAAACC TGAATCCTGT TTGGGAGGAG AAGTTCCATT TTGAGTGCCA CAACTCTTCT
    GACCGCATTA AGGTACGTGT GTGGGATGAG GATGATGACA TCAAGTCACG AGTAAAGCAG
    CGGCTAAAGC GAGAGTCTGA TGATTTCCTC GGCCAAACCA TCATTGAGGT TCGAACACTG
    AGTGGCGAAA TGGACGTCTG GTACAACTTG GAGAAGAGGA CAGACAAGTC AGCCGTCTCG
    GGGGCCATCC GACTGCAAAT CAGCGTGGAG ATCAAGGGAG AAGAGAAGGT AGCCCCATAT
    CATGTGCAGT ACACGTGTCT CCATGAGAAC CTCTTCCATT ACCTCACGGA CATTCAGGGC
    AGCGGAGGAG TCCAGATCCC TGAGGCTCGA GGAGATGATG CATGGAAGGT GTACTTTGAT
    GAGACTGCCC AGGAAATTGT GGATGAATTT GCCATGCGCT ATGGCATTGA GTCCATATAT
    CAGGCCATGA CGCATTTTGC ATGTTTGTCA TCCAAGTACA TGTGTCCTGG TGTGCCAGCA
    GTGATGAGCA CCTTACTGGC CAACATCAAT GCCTACTATG CCCACACAAC TGCCTCCACC
    AATGTCTCTG CATCTGATCG TTTTGCAGCC TCCAACTTTG GGAAAGAGAG ATTTGTGAAA
    CTTCTGGACC AGCTGCACAA CTCACTGAGG ATTGACCTCT CTACCTACAG GAATAATTTC
    CCTGCTGGGA GCCCTGAGCG ACTTCAGGAC TTAAAGTCCA CAGTGGATTT GCTGACCAGC
    ATTACTTTCT TCCGAATGAA GGTGCAAGAA CTTCAGAGCC CTCCAAGAGC CAGCCAGGTA
    GTAAAAGATT GTGTGAAGGC CTGTTTGAAC TCAACATATG AGTATATCTT CAACAACTGC
    CATGACTTGT ACAGCCGCCA GTACCAGCTG AATCAGGAGC TGCCTCCAGA AGAACAGGGG
    CCCAGCATTA GGAACCTGGA TTTCTGGCCC AAACTCATCA CACTCATTGT GTCAATCATA
    GAGGAAGATA AGAATTCCTA CACCCTCGTT CTGAACCAGT TTCCCCAGGA GTTGAATGTG
    GGAAAAGTCA GCGCAGAAGT GATGTGGCAA CTCTTTGCCC AAGACATGAA ATATGCACTG
    GAGGAGCATG AGAAAGACAG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG
    GTGAAGTGGC TCCACAATGA ATACGTGCAG GATCTGCCTG CCCTCCAGGG CCAGGTGCCC
    GAATACCCAG CGTGGTTTGA GCAGTTTGTC CTGCAGTGGC TGGACGAGAA TGAGGATGTG
    TCCTTGGAAT TCCTGCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA
    TCAGAGCACG CGCTCTTCTC TTGCTCTGTG GTGGACGTCT TCACACAGCT CAATCAGAGC
    TTTGAGATCA TCCGGAAATT GGAATGCCCA GACCCCAACA TCCTTGCCCA CTACATGAGG
    AGATTTTCTA AGACCATCGG GAAGGTGCTC ATGGAATATG CAGACATCTT ATCAAAGAAC
    TTCCCAGCTT ACTGCACAAA GGAGAAAATG CCTTGCATCC TGATGAACAA CATGCAGCAG
    CTGAGGGTGC AGCTGGAGAA GATGTTTGAG GCCATGGGAG GCAAGGAGCT GGACCCTGAA
    ACTGCAGACA GTCTGAAGGA GCTTCAGGTG AAACTGAATA CAGTTTTGGA TGAGCTCAGC
    ATGGTGTTTG GAAACAGTTT CCAGGTGCGG ATTGATGAGT GCGTGCGACA AATGGCCGAC
    ATCCTGAGCC AGGTGCGGGG CCCAGGGAAC GCATCCCCCA ACGCACGGGC CTCGGTGACT
    CAGGATGCAG ACAGTGTGCT CCGGCCTCTC ATGGACTTCC TGGACGGCAA CCTCACACTT
    TTTGCCACTG TGTGTGAGAA GACGGTCCTG AAGCGGGTAC TGAAGGAACT CTGGCGGGTG
    GTAATGAACA CAATGGAAAG GATGATTGTT CTGCCTCCAC TTACTGACCA GACGGGCACC
    CAGCTGATCT TCACTGCTGC CAAGGAGCTG AGCCAACTTT CCAAACTCAA GGACCACATG
    GTGCGAGAGG AAACACGGAA TCTGACTCCA AAGCAGTGTG CCGTCCTTGA CCTCGCCCTG
    GACACCATCA AGCAATACTT TCACGCCGGA GGCAACGGGC TGAAGAAAAC CTTCCTGGAG
    AAGAGCCCAG ATCTGCAGTC CCTGCGCTAT GCCCTATCTC TGTACACACA GACCACAGAC
    ACCCTCATCA AGACCTTTGT GCGCTCACAG ACTGCCCAGG GGTCCGGTGT GGATGATCCT
    GTGGGAGAAG TCTCTATTCA GGTGGACTTG TTTACACATC CTGGAACTGG GGAGCACAAG
    GTCACAGTGA AAGTGGTGGC TGCCAATGAC CTCAAGTGGC AGACAGCAGG TATGTTCCGG
    CCCTTTGTCG AAGTCACCAT GGTTGGCCCT CACCAAAGTG ATAAAAAGAG GAAGTTCACA
    ACCAAGTCAA AAAGTAACAA CTGGGCTCCC AAGTACAACG AGACGTTTCA CTTCCTCCTG
    GGAAATGAAG AGGGGCCAGA AGCCTATGAG TTGCAGATAT GCGTGAAGGA TTACTGCTTT
    GCCCGGGAGG ACCGTGTGCT GGGGCTGGCT GTAATGCCTC TGAGGGATGT GGCAGCCAAG
    GGCAGCTGTG CTTGCTGGTG CCCCTTGGGC CGTAAGATCC ACATGGATGA GACAGGCGTG
    ACCATTCTCC GGATTCTGTC TCAGAGGAGC AATGACGAAG TGGCCCGGGA GTTTGTGAAG
    CTCAAATCAG AGTCTCGTTC TATGGAGGAG GGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328009.1
    CDS377..6295
    Translation

    Target ORF information:

    RefSeq Version XM_015472523.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GAGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGACAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG GCTATGTCAC TGGTCCAGTC CCGGAAAGCA
    GGCATTACTT CTGCAATGGC TACACGCACC TCTCTCAAAG ATGAAGAGCT GAAATCACAT
    GTGTATAAGA AAACCCTGCA GGCCTTAATC TATCCCATCT CGTGCACCAC ACCCCACAAC
    TTTGAGGTGT GGTCAGCCAC CACCCCCACC TACTGCTATG AGTGTGAAGG CCTGCTCTGG
    GGCATTGCCC GGCAGGGCAT GCGCTGCAGC GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC
    CAGGACCTAC TCAATGCTGA CTGCCTGCAG CGGGCTGCAG AAAAGAGCTC TAAACATGGA
    GCGGAGGACC GGACCCAGAA CATCATCATG GCCATGAAGG ACCGCATGAA GATCCGAGAG
    CGGAATAAGC CAGAGATCTT TGAAGTTATC CGGGATGTCT TCAATGTGAG CAAAGTCGCC
    CATGTGCAAC AAATGAAAAC AGTGAAGCAG AGTGTGTTGG ATGGCACCTC CAAATGGTCA
    GCCAAAATCA CCATCACTGT GGTGTGTGCC CAGGGCCTAC AAGCCAAGGA CAAGACAGGA
    TCCAGTGACC CTTATGTGAC TGTGCAAGTG GGCAAAACCA AGAAGCGTAC CAAGACCATT
    TTTGGAAACC TGAATCCTGT TTGGGAGGAG AAGTTCCATT TTGAGTGCCA CAACTCTTCT
    GACCGCATTA AGGTACGTGT GTGGGATGAG GATGATGACA TCAAGTCACG AGTAAAGCAG
    CGGCTAAAGC GAGAGTCTGA TGATTTCCTC GGCCAAACCA TCATTGAGGT TCGAACACTG
    AGTGGCGAAA TGGACGTCTG GTACAACTTG GAGAAGAGGA CAGACAAGTC AGCCGTCTCG
    GGGGCCATCC GACTGCAAAT CAGCGTGGAG ATCAAGGGAG AAGAGAAGGT AGCCCCATAT
    CATGTGCAGT ACACGTGTCT CCATGAGAAC CTCTTCCATT ACCTCACGGA CATTCAGGGC
    AGCGGAGGAG TCCAGATCCC TGAGGCTCGA GGAGATGATG CATGGAAGGT GTACTTTGAT
    GAGACTGCCC AGGAAATTGT GGATGAATTT GCCATGCGCT ATGGCATTGA GTCCATATAT
    CAGGCCATGA CGCATTTTGC ATGTTTGTCA TCCAAGTACA TGTGTCCTGG TGTGCCAGCA
    GTGATGAGCA CCTTACTGGC CAACATCAAT GCCTACTATG CCCACACAAC TGCCTCCACC
    AATGTCTCTG CATCTGATCG TTTTGCAGCC TCCAACTTTG GGAAAGAGAG ATTTGTGAAA
    CTTCTGGACC AGCTGCACAA CTCACTGAGG ATTGACCTCT CTACCTACAG GAATAATTTC
    CCTGCTGGGA GCCCTGAGCG ACTTCAGGAC TTAAAGTCCA CAGTGGATTT GCTGACCAGC
    ATTACTTTCT TCCGAATGAA GGTGCAAGAA CTTCAGAGCC CTCCAAGAGC CAGCCAGGTA
    GTAAAAGATT GTGTGAAGGC CTGTTTGAAC TCAACATATG AGTATATCTT CAACAACTGC
    CATGACTTGT ACAGCCGCCA GTACCAGCTG AATCAGGAGC TGCCTCCAGA AGAACAGGGG
    CCCAGCATTA GGAACCTGGA TTTCTGGCCC AAACTCATCA CACTCATTGT GTCAATCATA
    GAGGAAGATA AGAATTCCTA CACCCTCGTT CTGAACCAGT TTCCCCAGGA GTTGAATGTG
    GGAAAAGTCA GCGCAGAAGT GATGTGGCAA CTCTTTGCCC AAGACATGAA ATATGCACTG
    GAGGAGCATG AGAAAGACAG GCTGTGTAAG AGTGCTGACT ACATGAACCT GCACTTCAAG
    GTGAAGTGGC TCCACAATGA ATACGTGCAG GATCTGCCTG CCCTCCAGGG CCAGGTGCCC
    GAATACCCAG CGTGGTTTGA GCAGTTTGTC CTGCAGTGGC TGGACGAGAA TGAGGATGTG
    TCCTTGGAAT TCCTGCGTGG GGCCTTGGAA CGAGATAAGA AGGATGGGTT CCAGCAGACA
    TCAGAGCACG CGCTCTTCTC TTGCTCTGTG GTGGACGTCT TCACACAGCT CAATCAGAGC
    TTTGAGATCA TCCGGAAATT GGAATGCCCA GACCCCAACA TCCTTGCCCA CTACATGAGG
    AGATTTTCTA AGACCATCGG GAAGGTGCTC ATGGAATATG CAGACATCTT ATCAAAGAAC
    TTCCCAGCTT ACTGCACAAA GGAGAAAATG CCTTGCATCC TGATGAACAA CATGCAGCAG
    CTGAGGGTGC AGCTGGAGAA GATGTTTGAG GCCATGGGAG GCAAGGAGCT GGACCCTGAA
    ACTGCAGACA GTCTGAAGGA GCTTCAGGTG AAACTGAATA CAGTTTTGGA TGAGCTCAGC
    ATGGTGTTTG GAAACAGTTT CCAGGTGCGG ATTGATGAGT GCGTGCGACA AATGGCCGAC
    ATCCTGAGCC AGGTGCGGGG CCCAGGGAAC GCATCCCCCA ACGCACGGGC CTCGGTGACT
    CAGGATGCAG ACAGTGTGCT CCGGCCTCTC ATGGACTTCC TGGACGGCAA CCTCACACTT
    TTTGCCACTG TGTGTGAGAA GACGGTCCTG AAGCGGGTAC TGAAGGAACT CTGGCGGGTG
    GTAATGAACA CAATGGAAAG GATGATTGTT CTGCCTCCAC TTACTGACCA GACGGGCACC
    CAGCTGATCT TCACTGCTGC CAAGGAGCTG AGCCAACTTT CCAAACTCAA GGACCACATG
    GTGCGAGAGG AAACACGGAA TCTGACTCCA AAGCAGTGTG CCGTCCTTGA CCTCGCCCTG
    GACACCATCA AGCAATACTT TCACGCCGGA GGCAACGGGC TGAAGAAAAC CTTCCTGGAG
    AAGAGCCCAG ATCTGCAGTC CCTGCGCTAT GCCCTATCTC TGTACACACA GACCACAGAC
    ACCCTCATCA AGACCTTTGT GCGCTCACAG ACTGCCCAGG GGTCCGGTGT GGATGATCCT
    GTGGGAGAAG TCTCTATTCA GGTGGACTTG TTTACACATC CTGGAACTGG GGAGCACAAG
    GTCACAGTGA AAGTGGTGGC TGCCAATGAC CTCAAGTGGC AGACAGCAGG TATGTTCCGG
    CCCTTTGTCG AAGTCACCAT GGTTGGCCCT CACCAAAGTG ATAAAAAGAG GAAGTTCACA
    ACCAAGTCAA AAAGTAACAA CTGGGCTCCC AAGTACAACG AGACGTTTCA CTTCCTCCTG
    GGAAATGAAG AGGGGCCAGA AGCCTATGAG TTGCAGATAT GCGTGAAGGA TTACTGCTTT
    GCCCGGGAGG ACCGTGTGCT GGGGCTGGCT GTAATGCCTC TGAGGGATGT GGCAGCCAAG
    GGCAGCTGTG CTTGCTGGTG CCCCTTGGGC CGTAAGATCC ACATGGATGA GACAGGCGTG
    ACCATTCTCC GGATTCTGTC TCAGAGGAGC AATGACGAAG TGGCCCGGGA GTTTGTGAAG
    CTCAAATCAG AGTCTCGTTC TATGGAGGAG GGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91273
    Clone ID Related Accession (Same CDS sequence) XM_015472524.1
    Accession Version XM_015472524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGAAG GAAAGCGAGG AGCCCTTCAG GTTGTGCTCT
    GGCCCTTTGG GGCCCCTTTG TTGTTGGTTC TCTCCCCTCC CTCCTGAGTA CAATATTCCA
    GATGGTGGTG ATCCCTGTAT GCCCCAGTGG CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT
    GGCATTGACA GCATGCCGGA TCTACGCAGA AAGAAGCCAC TGCCGCTTGT CAGCGACCTG
    GCTATGTCAC TGGTCCAGTC CCGGAAAGCA GGCATTACTT CTGCAATGGC TACACGCACC
    TCTCTCAAAG ATGAAGAGCT GAAATCACAT GTGTATAAGA AAACCCTGCA GGCCTTAATC
    TATCCCATCT CGTGCACCAC ACCCCACAAC TTTGAGGTGT GGTCAGCCAC CACCCCCACC
    TACTGCTATG AGTGTGAAGG CCTGCTCTGG GGCATTGCCC GGCAGGGCAT GCGCTGCAGC
    GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC CAGGACCTAC TCAATGCTGA CTGCCTGCAG
    CGGGCTGCAG AAAAGAGCTC TAAACATGGA GCGGAGGACC GGACCCAGAA CATCATCATG
    GCCATGAAGG ACCGCATGAA GATCCGAGAG CGGAATAAGC CAGAGATCTT TGAAGTTATC
    CGGGATGTCT TCAATGTGAG CAAAGTCGCC CATGTGCAAC AAATGAAAAC AGTGAAGCAG
    AGTGTGTTGG ATGGCACCTC CAAATGGTCA GCCAAAATCA CCATCACTGT GGTGTGTGCC
    CAGGGCCTAC AAGCCAAGGA CAAGACAGGA TCCAGTGACC CTTATGTGAC TGTGCAAGTG
    GGCAAAACCA AGAAGCGTAC CAAGACCATT TTTGGAAACC TGAATCCTGT TTGGGAGGAG
    AAGTTCCATT TTGAGTGCCA CAACTCTTCT GACCGCATTA AGGTACGTGT GTGGGATGAG
    GATGATGACA TCAAGTCACG AGTAAAGCAG CGGCTAAAGC GAGAGTCTGA TGATTTCCTC
    GGCCAAACCA TCATTGAGGT TCGAACACTG AGTGGCGAAA TGGACGTCTG GTACAACTTG
    GAGAAGAGGA CAGACAAGTC AGCCGTCTCG GGGGCCATCC GACTGCAAAT CAGCGTGGAG
    ATCAAGGGAG AAGAGAAGGT AGCCCCATAT CATGTGCAGT ACACGTGTCT CCATGAGAAC
    CTCTTCCATT ACCTCACGGA CATTCAGGGC AGCGGAGGAG TCCAGATCCC TGAGGCTCGA
    GGAGATGATG CATGGAAGGT GTACTTTGAT GAGACTGCCC AGGAAATTGT GGATGAATTT
    GCCATGCGCT ATGGCATTGA GTCCATATAT CAGGCCATGA CGCATTTTGC ATGTTTGTCA
    TCCAAGTACA TGTGTCCTGG TGTGCCAGCA GTGATGAGCA CCTTACTGGC CAACATCAAT
    GCCTACTATG CCCACACAAC TGCCTCCACC AATGTCTCTG CATCTGATCG TTTTGCAGCC
    TCCAACTTTG GGAAAGAGAG ATTTGTGAAA CTTCTGGACC AGCTGCACAA CTCACTGAGG
    ATTGACCTCT CTACCTACAG GAATAATTTC CCTGCTGGGA GCCCTGAGCG ACTTCAGGAC
    TTAAAGTCCA CAGTGGATTT GCTGACCAGC ATTACTTTCT TCCGAATGAA GGTGCAAGAA
    CTTCAGAGCC CTCCAAGAGC CAGCCAGGTA GTAAAAGATT GTGTGAAGGC CTGTTTGAAC
    TCAACATATG AGTATATCTT CAACAACTGC CATGACTTGT ACAGCCGCCA GTACCAGCTG
    AATCAGGAGC TGCCTCCAGA AGAACAGGGG CCCAGCATTA GGAACCTGGA TTTCTGGCCC
    AAACTCATCA CACTCATTGT GTCAATCATA GAGGAAGATA AGAATTCCTA CACCCTCGTT
    CTGAACCAGT TTCCCCAGGA GTTGAATGTG GGAAAAGTCA GCGCAGAAGT GATGTGGCAA
    CTCTTTGCCC AAGACATGAA ATATGCACTG GAGGAGCATG AGAAAGACAG GCTGTGTAAG
    AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATACGTGCAG
    GATCTGCCTG CCCTCCAGGG CCAGGTGCCC GAATACCCAG CGTGGTTTGA GCAGTTTGTC
    CTGCAGTGGC TGGACGAGAA TGAGGATGTG TCCTTGGAAT TCCTGCGTGG GGCCTTGGAA
    CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCACG CGCTCTTCTC TTGCTCTGTG
    GTGGACGTCT TCACACAGCT CAATCAGAGC TTTGAGATCA TCCGGAAATT GGAATGCCCA
    GACCCCAACA TCCTTGCCCA CTACATGAGG AGATTTTCTA AGACCATCGG GAAGGTGCTC
    ATGGAATATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAAAATG
    CCTTGCATCC TGATGAACAA CATGCAGCAG CTGAGGGTGC AGCTGGAGAA GATGTTTGAG
    GCCATGGGAG GCAAGGAGCT GGACCCTGAA ACTGCAGACA GTCTGAAGGA GCTTCAGGTG
    AAACTGAATA CAGTTTTGGA TGAGCTCAGC ATGGTGTTTG GAAACAGTTT CCAGGTGCGG
    ATTGATGAGT GCGTGCGACA AATGGCCGAC ATCCTGAGCC AGGTGCGGGG CCCAGGGAAC
    GCATCCCCCA ACGCACGGGC CTCGGTGACT CAGGATGCAG ACAGTGTGCT CCGGCCTCTC
    ATGGACTTCC TGGACGGCAA CCTCACACTT TTTGCCACTG TGTGTGAGAA GACGGTCCTG
    AAGCGGGTAC TGAAGGAACT CTGGCGGGTG GTAATGAACA CAATGGAAAG GATGATTGTT
    CTGCCTCCAC TTACTGACCA GACGGGCACC CAGCTGATCT TCACTGCTGC CAAGGAGCTG
    AGCCAACTTT CCAAACTCAA GGACCACATG GTGCGAGAGG AAACACGGAA TCTGACTCCA
    AAGCAGTGTG CCGTCCTTGA CCTCGCCCTG GACACCATCA AGCAATACTT TCACGCCGGA
    GGCAACGGGC TGAAGAAAAC CTTCCTGGAG AAGAGCCCAG ATCTGCAGTC CCTGCGCTAT
    GCCCTATCTC TGTACACACA GACCACAGAC ACCCTCATCA AGACCTTTGT GCGCTCACAG
    ACTGCCCAGG TGCATGATGG GAAAGGTATT AGGTTTACTG CTAATGAGGA CATTCAGCCG
    GAAAAGGGGT CCGGTGTGGA TGATCCTGTG GGAGAAGTCT CTATTCAGGT GGACTTGTTT
    ACACATCCTG GAACTGGGGA GCACAAGGTC ACAGTGAAAG TGGTGGCTGC CAATGACCTC
    AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC TTTGTCGAAG TCACCATGGT TGGCCCTCAC
    CAAAGTGATA AAAAGAGGAA GTTCACAACC AAGTCAAAAA GTAACAACTG GGCTCCCAAG
    TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GGCCAGAAGC CTATGAGTTG
    CAGATATGCG TGAAGGATTA CTGCTTTGCC CGGGAGGACC GTGTGCTGGG GCTGGCTGTA
    ATGCCTCTGA GGGATGTGGC AGCCAAGGGC AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT
    AAGATCCACA TGGATGAGAC AGGCGTGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT
    GACGAAGTGG CCCGGGAGTT TGTGAAGCTC AAATCAGAGT CTCGTTCTAT GGAGGAGGGG
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328010.1
    CDS331..5256
    Translation

    Target ORF information:

    RefSeq Version XM_015472524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGAAG GAAAGCGAGG AGCCCTTCAG GTTGTGCTCT
    GGCCCTTTGG GGCCCCTTTG TTGTTGGTTC TCTCCCCTCC CTCCTGAGTA CAATATTCCA
    GATGGTGGTG ATCCCTGTAT GCCCCAGTGG CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT
    GGCATTGACA GCATGCCGGA TCTACGCAGA AAGAAGCCAC TGCCGCTTGT CAGCGACCTG
    GCTATGTCAC TGGTCCAGTC CCGGAAAGCA GGCATTACTT CTGCAATGGC TACACGCACC
    TCTCTCAAAG ATGAAGAGCT GAAATCACAT GTGTATAAGA AAACCCTGCA GGCCTTAATC
    TATCCCATCT CGTGCACCAC ACCCCACAAC TTTGAGGTGT GGTCAGCCAC CACCCCCACC
    TACTGCTATG AGTGTGAAGG CCTGCTCTGG GGCATTGCCC GGCAGGGCAT GCGCTGCAGC
    GAGTGTGGAG TCAAGTGCCA TGAGAAGTGC CAGGACCTAC TCAATGCTGA CTGCCTGCAG
    CGGGCTGCAG AAAAGAGCTC TAAACATGGA GCGGAGGACC GGACCCAGAA CATCATCATG
    GCCATGAAGG ACCGCATGAA GATCCGAGAG CGGAATAAGC CAGAGATCTT TGAAGTTATC
    CGGGATGTCT TCAATGTGAG CAAAGTCGCC CATGTGCAAC AAATGAAAAC AGTGAAGCAG
    AGTGTGTTGG ATGGCACCTC CAAATGGTCA GCCAAAATCA CCATCACTGT GGTGTGTGCC
    CAGGGCCTAC AAGCCAAGGA CAAGACAGGA TCCAGTGACC CTTATGTGAC TGTGCAAGTG
    GGCAAAACCA AGAAGCGTAC CAAGACCATT TTTGGAAACC TGAATCCTGT TTGGGAGGAG
    AAGTTCCATT TTGAGTGCCA CAACTCTTCT GACCGCATTA AGGTACGTGT GTGGGATGAG
    GATGATGACA TCAAGTCACG AGTAAAGCAG CGGCTAAAGC GAGAGTCTGA TGATTTCCTC
    GGCCAAACCA TCATTGAGGT TCGAACACTG AGTGGCGAAA TGGACGTCTG GTACAACTTG
    GAGAAGAGGA CAGACAAGTC AGCCGTCTCG GGGGCCATCC GACTGCAAAT CAGCGTGGAG
    ATCAAGGGAG AAGAGAAGGT AGCCCCATAT CATGTGCAGT ACACGTGTCT CCATGAGAAC
    CTCTTCCATT ACCTCACGGA CATTCAGGGC AGCGGAGGAG TCCAGATCCC TGAGGCTCGA
    GGAGATGATG CATGGAAGGT GTACTTTGAT GAGACTGCCC AGGAAATTGT GGATGAATTT
    GCCATGCGCT ATGGCATTGA GTCCATATAT CAGGCCATGA CGCATTTTGC ATGTTTGTCA
    TCCAAGTACA TGTGTCCTGG TGTGCCAGCA GTGATGAGCA CCTTACTGGC CAACATCAAT
    GCCTACTATG CCCACACAAC TGCCTCCACC AATGTCTCTG CATCTGATCG TTTTGCAGCC
    TCCAACTTTG GGAAAGAGAG ATTTGTGAAA CTTCTGGACC AGCTGCACAA CTCACTGAGG
    ATTGACCTCT CTACCTACAG GAATAATTTC CCTGCTGGGA GCCCTGAGCG ACTTCAGGAC
    TTAAAGTCCA CAGTGGATTT GCTGACCAGC ATTACTTTCT TCCGAATGAA GGTGCAAGAA
    CTTCAGAGCC CTCCAAGAGC CAGCCAGGTA GTAAAAGATT GTGTGAAGGC CTGTTTGAAC
    TCAACATATG AGTATATCTT CAACAACTGC CATGACTTGT ACAGCCGCCA GTACCAGCTG
    AATCAGGAGC TGCCTCCAGA AGAACAGGGG CCCAGCATTA GGAACCTGGA TTTCTGGCCC
    AAACTCATCA CACTCATTGT GTCAATCATA GAGGAAGATA AGAATTCCTA CACCCTCGTT
    CTGAACCAGT TTCCCCAGGA GTTGAATGTG GGAAAAGTCA GCGCAGAAGT GATGTGGCAA
    CTCTTTGCCC AAGACATGAA ATATGCACTG GAGGAGCATG AGAAAGACAG GCTGTGTAAG
    AGTGCTGACT ACATGAACCT GCACTTCAAG GTGAAGTGGC TCCACAATGA ATACGTGCAG
    GATCTGCCTG CCCTCCAGGG CCAGGTGCCC GAATACCCAG CGTGGTTTGA GCAGTTTGTC
    CTGCAGTGGC TGGACGAGAA TGAGGATGTG TCCTTGGAAT TCCTGCGTGG GGCCTTGGAA
    CGAGATAAGA AGGATGGGTT CCAGCAGACA TCAGAGCACG CGCTCTTCTC TTGCTCTGTG
    GTGGACGTCT TCACACAGCT CAATCAGAGC TTTGAGATCA TCCGGAAATT GGAATGCCCA
    GACCCCAACA TCCTTGCCCA CTACATGAGG AGATTTTCTA AGACCATCGG GAAGGTGCTC
    ATGGAATATG CAGACATCTT ATCAAAGAAC TTCCCAGCTT ACTGCACAAA GGAGAAAATG
    CCTTGCATCC TGATGAACAA CATGCAGCAG CTGAGGGTGC AGCTGGAGAA GATGTTTGAG
    GCCATGGGAG GCAAGGAGCT GGACCCTGAA ACTGCAGACA GTCTGAAGGA GCTTCAGGTG
    AAACTGAATA CAGTTTTGGA TGAGCTCAGC ATGGTGTTTG GAAACAGTTT CCAGGTGCGG
    ATTGATGAGT GCGTGCGACA AATGGCCGAC ATCCTGAGCC AGGTGCGGGG CCCAGGGAAC
    GCATCCCCCA ACGCACGGGC CTCGGTGACT CAGGATGCAG ACAGTGTGCT CCGGCCTCTC
    ATGGACTTCC TGGACGGCAA CCTCACACTT TTTGCCACTG TGTGTGAGAA GACGGTCCTG
    AAGCGGGTAC TGAAGGAACT CTGGCGGGTG GTAATGAACA CAATGGAAAG GATGATTGTT
    CTGCCTCCAC TTACTGACCA GACGGGCACC CAGCTGATCT TCACTGCTGC CAAGGAGCTG
    AGCCAACTTT CCAAACTCAA GGACCACATG GTGCGAGAGG AAACACGGAA TCTGACTCCA
    AAGCAGTGTG CCGTCCTTGA CCTCGCCCTG GACACCATCA AGCAATACTT TCACGCCGGA
    GGCAACGGGC TGAAGAAAAC CTTCCTGGAG AAGAGCCCAG ATCTGCAGTC CCTGCGCTAT
    GCCCTATCTC TGTACACACA GACCACAGAC ACCCTCATCA AGACCTTTGT GCGCTCACAG
    ACTGCCCAGG TGCATGATGG GAAAGGTATT AGGTTTACTG CTAATGAGGA CATTCAGCCG
    GAAAAGGGGT CCGGTGTGGA TGATCCTGTG GGAGAAGTCT CTATTCAGGT GGACTTGTTT
    ACACATCCTG GAACTGGGGA GCACAAGGTC ACAGTGAAAG TGGTGGCTGC CAATGACCTC
    AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC TTTGTCGAAG TCACCATGGT TGGCCCTCAC
    CAAAGTGATA AAAAGAGGAA GTTCACAACC AAGTCAAAAA GTAACAACTG GGCTCCCAAG
    TACAACGAGA CGTTTCACTT CCTCCTGGGA AATGAAGAGG GGCCAGAAGC CTATGAGTTG
    CAGATATGCG TGAAGGATTA CTGCTTTGCC CGGGAGGACC GTGTGCTGGG GCTGGCTGTA
    ATGCCTCTGA GGGATGTGGC AGCCAAGGGC AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT
    AAGATCCACA TGGATGAGAC AGGCGTGACC ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT
    GACGAAGTGG CCCGGGAGTT TGTGAAGCTC AAATCAGAGT CTCGTTCTAT GGAGGAGGGG
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91274
    Clone ID Related Accession (Same CDS sequence) XM_015472525.1
    Accession Version XM_015472525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACAGTTA AAAGAGCCAA ATTCCAGGGT TCACCAGACA AATTTAACAC ATATGTGACC 
    CTGAAAGTGC AAAATGTGAA GAGTACAACT GTAGCAGTTC GTGGCGATCA ACCTTCCTGG
    GAACAAGATT TTATGTTTGA GATTAGTCGC CTGGACCTGG GTCTCAGTGT GGAAGTATGG
    AACAAAGGAC TGATCTGGGA CACCATGGTG GGGACTGTGT GGATTGCACT GAAGACGATT
    CGTCAGTCGG ATGAGGAAGG GCCTGGGGAA TGGTCCACGT TGGAGGCAGA GACATTAATG
    AAAGATGATG AAATCTGTGG AACTAAAAAC CCAACTCCCC ATAAAATTTT GCTTGATACA
    AGATTTGAGT TGCCTTTTGA TATCCCAGAA GAGGAAGCTA GATATTGGAC CTACAGATTG
    GAGCAAATGA ATGCCTTGGG ACCTGACAAT GAGTATTCTA GTCAAGAAGA AAGCCAGAGG
    AAGCAATTGC CTACTGCTGC CGCCCAGTGT TCTTTTGAAG ATCCTGACAG TGCCGTGGAT
    GACCGAGATA GTGATTATCG CAGTGAGACC AGCAATAGCA TCCCGCCTCC TTACCATACA
    ACTGCCCAGC CCAACGCTTC TGTGCACCAG TTCCCAGTGC CTGTGCGATT GCCACAGCAG
    CTGCTGCTTC AGGGCAGTTC CCGGGACTCT TGCAATGACT CTGTGCAAAG TTATGACCTT
    GAGTATCCAG AGCGGCGGGC CCTCAGCCCC ACCAGCAGTA GTAGGTATGG CTCCTCCTGT
    AACGTGAGTC AAGGAAGCTC TCAGTTAAGT GAACTGGACC AGTGTCATGA ACAAGATGAT
    GACCGTCGGG AGAGGGACTC CATTCATTCC TGCCACAGCT CCAGCAGCCT CTCCAGGGAT
    GGCCAAGTGG GTTTTGGAGA ACAAGAGAAA GCCTTGGAGG TGACAGGTGA AGAGGAGAAG
    AGGGGAGTGT GTGAACCCAA GGAGATGAAA GAAGATGCCA TGGCCCATCC ACCCCCAGAT
    ATGGTGCTAC ACAAAGACCT TGTCCGAAGC CCCCAGGAGA GTTTTCCTGA GGAGACTGCA
    TCTTCGCCAT TTACCCAAGC CAGAGCGCAC TGGATCCGAG CAGTTACCAA GGTTCGACTC
    CAGCTGCAGG AGAAGGAAAG CGAGGAGCCC TTCAGGTTGT GCTCTGGCCC TTTGGGGCCC
    CTTTGTTGTT GGTTCTCTCC CCTCCCTCCT GAGTACAATA TTCCAGATGG TGGTGATCCC
    TGTATGCCCC AGTGGCTTCC AGAAGGGCCA GCTGGAGGGC TCTATGGCAT TGACAGCATG
    CCGGATCTAC GCAGAAAGAA GCCACTGCCG CTTGTCAGCG ACCTGGCTAT GTCACTGGTC
    CAGTCCCGGA AAGCAGGCAT TACTTCTGCA ATGGCTACAC GCACCTCTCT CAAAGATGAA
    GAGCTGAAAT CACATGTGTA TAAGAAAACC CTGCAGGCCT TAATCTATCC CATCTCGTGC
    ACCACACCCC ACAACTTTGA GGTGTGGTCA GCCACCACCC CCACCTACTG CTATGAGTGT
    GAAGGCCTGC TCTGGGGCAT TGCCCGGCAG GGCATGCGCT GCAGCGAGTG TGGAGTCAAG
    TGCCATGAGA AGTGCCAGGA CCTACTCAAT GCTGACTGCC TGCAGCGGGC TGCAGAAAAG
    AGCTCTAAAC ATGGAGCGGA GGACCGGACC CAGAACATCA TCATGGCCAT GAAGGACCGC
    ATGAAGATCC GAGAGCGGAA TAAGCCAGAG ATCTTTGAAG TTATCCGGGA TGTCTTCAAT
    GTGAGCAAAG TCGCCCATGT GCAACAAATG AAAACAGTGA AGCAGAGTGT GTTGGATGGC
    ACCTCCAAAT GGTCAGCCAA AATCACCATC ACTGTGGTGT GTGCCCAGGG CCTACAAGCC
    AAGGACAAGA CAGGATCCAG TGACCCTTAT GTGACTGTGC AAGTGGGCAA AACCAAGAAG
    CGTACCAAGA CCATTTTTGG AAACCTGAAT CCTGTTTGGG AGGAGAAGTT CCATTTTGAG
    TGCCACAACT CTTCTGACCG CATTAAGGTA CGTGTGTGGG ATGAGGATGA TGACATCAAG
    TCACGAGTAA AGCAGCGGCT AAAGCGAGAG TCTGATGATT TCCTCGGCCA AACCATCATT
    GAGGTTCGAA CACTGAGTGG CGAAATGGAC GTCTGGTACA ACTTGGAGAA GAGGACAGAC
    AAGTCAGCCG TCTCGGGGGC CATCCGACTG CAAATCAGCG TGGAGATCAA GGGAGAAGAG
    AAGGTAGCCC CATATCATGT GCAGTACACG TGTCTCCATG AGAACCTCTT CCATTACCTC
    ACGGACATTC AGGGCAGCGG AGGAGTCCAG ATCCCTGAGG CTCGAGGAGA TGATGCATGG
    AAGGTGTACT TTGATGAGAC TGCCCAGGAA ATTGTGGATG AATTTGCCAT GCGCTATGGC
    ATTGAGTCCA TATATCAGGC CATGACGCAT TTTGCATGTT TGTCATCCAA GTACATGTGT
    CCTGGTGTGC CAGCAGTGAT GAGCACCTTA CTGGCCAACA TCAATGCCTA CTATGCCCAC
    ACAACTGCCT CCACCAATGT CTCTGCATCT GATCGTTTTG CAGCCTCCAA CTTTGGGAAA
    GAGAGATTTG TGAAACTTCT GGACCAGCTG CACAACTCAC TGAGGATTGA CCTCTCTACC
    TACAGGAATA ATTTCCCTGC TGGGAGCCCT GAGCGACTTC AGGACTTAAA GTCCACAGTG
    GATTTGCTGA CCAGCATTAC TTTCTTCCGA ATGAAGGTGC AAGAACTTCA GAGCCCTCCA
    AGAGCCAGCC AGGTAGTAAA AGATTGTGTG AAGGCCTGTT TGAACTCAAC ATATGAGTAT
    ATCTTCAACA ACTGCCATGA CTTGTACAGC CGCCAGTACC AGCTGAATCA GGAGCTGCCT
    CCAGAAGAAC AGGGGCCCAG CATTAGGAAC CTGGATTTCT GGCCCAAACT CATCACACTC
    ATTGTGTCAA TCATAGAGGA AGATAAGAAT TCCTACACCC TCGTTCTGAA CCAGTTTCCC
    CAGGAGTTGA ATGTGGGAAA AGTCAGCGCA GAAGTGATGT GGCAACTCTT TGCCCAAGAC
    ATGAAATATG CACTGGAGGA GCATGAGAAA GACAGGCTGT GTAAGAGTGC TGACTACATG
    AACCTGCACT TCAAGGTGAA GTGGCTCCAC AATGAATACG TGCAGGATCT GCCTGCCCTC
    CAGGGCCAGG TGCCCGAATA CCCAGCGTGG TTTGAGCAGT TTGTCCTGCA GTGGCTGGAC
    GAGAATGAGG ATGTGTCCTT GGAATTCCTG CGTGGGGCCT TGGAACGAGA TAAGAAGGAT
    GGGTTCCAGC AGACATCAGA GCACGCGCTC TTCTCTTGCT CTGTGGTGGA CGTCTTCACA
    CAGCTCAATC AGAGCTTTGA GATCATCCGG AAATTGGAAT GCCCAGACCC CAACATCCTT
    GCCCACTACA TGAGGAGATT TTCTAAGACC ATCGGGAAGG TGCTCATGGA ATATGCAGAC
    ATCTTATCAA AGAACTTCCC AGCTTACTGC ACAAAGGAGA AAATGCCTTG CATCCTGATG
    AACAACATGC AGCAGCTGAG GGTGCAGCTG GAGAAGATGT TTGAGGCCAT GGGAGGCAAG
    GAGCTGGACC CTGAAACTGC AGACAGTCTG AAGGAGCTTC AGGTGAAACT GAATACAGTT
    TTGGATGAGC TCAGCATGGT GTTTGGAAAC AGTTTCCAGG TGCGGATTGA TGAGTGCGTG
    CGACAAATGG CCGACATCCT GAGCCAGGTG CGGGGCCCAG GGAACGCATC CCCCAACGCA
    CGGGCCTCGG TGACTCAGGA TGCAGACAGT GTGCTCCGGC CTCTCATGGA CTTCCTGGAC
    GGCAACCTCA CACTTTTTGC CACTGTGTGT GAGAAGACGG TCCTGAAGCG GGTACTGAAG
    GAACTCTGGC GGGTGGTAAT GAACACAATG GAAAGGATGA TTGTTCTGCC TCCACTTACT
    GACCAGACGG GCACCCAGCT GATCTTCACT GCTGCCAAGG AGCTGAGCCA ACTTTCCAAA
    CTCAAGGACC ACATGGTGCG AGAGGAAACA CGGAATCTGA CTCCAAAGCA GTGTGCCGTC
    CTTGACCTCG CCCTGGACAC CATCAAGCAA TACTTTCACG CCGGAGGCAA CGGGCTGAAG
    AAAACCTTCC TGGAGAAGAG CCCAGATCTG CAGTCCCTGC GCTATGCCCT ATCTCTGTAC
    ACACAGACCA CAGACACCCT CATCAAGACC TTTGTGCGCT CACAGACTGC CCAGGTGCAT
    GATGGGAAAG GTATTAGGTT TACTGCTAAT GAGGACATTC AGCCGGAAAA GGGGTCCGGT
    GTGGATGATC CTGTGGGAGA AGTCTCTATT CAGGTGGACT TGTTTACACA TCCTGGAACT
    GGGGAGCACA AGGTCACAGT GAAAGTGGTG GCTGCCAATG ACCTCAAGTG GCAGACAGCA
    GGTATGTTCC GGCCCTTTGT CGAAGTCACC ATGGTTGGCC CTCACCAAAG TGATAAAAAG
    AGGAAGTTCA CAACCAAGTC AAAAAGTAAC AACTGGGCTC CCAAGTACAA CGAGACGTTT
    CACTTCCTCC TGGGAAATGA AGAGGGGCCA GAAGCCTATG AGTTGCAGAT ATGCGTGAAG
    GATTACTGCT TTGCCCGGGA GGACCGTGTG CTGGGGCTGG CTGTAATGCC TCTGAGGGAT
    GTGGCAGCCA AGGGCAGCTG TGCTTGCTGG TGCCCCTTGG GCCGTAAGAT CCACATGGAT
    GAGACAGGCG TGACCATTCT CCGGATTCTG TCTCAGAGGA GCAATGACGA AGTGGCCCGG
    GAGTTTGTGA AGCTCAAATC AGAGTCTCGT TCTATGGAGG AGGGGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328011.1
    CDS86..4996
    Translation

    Target ORF information:

    RefSeq Version XM_015472525.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACAGTTA AAAGAGCCAA ATTCCAGGGT TCACCAGACA AATTTAACAC ATATGTGACC 
    CTGAAAGTGC AAAATGTGAA GAGTACAACT GTAGCAGTTC GTGGCGATCA ACCTTCCTGG
    GAACAAGATT TTATGTTTGA GATTAGTCGC CTGGACCTGG GTCTCAGTGT GGAAGTATGG
    AACAAAGGAC TGATCTGGGA CACCATGGTG GGGACTGTGT GGATTGCACT GAAGACGATT
    CGTCAGTCGG ATGAGGAAGG GCCTGGGGAA TGGTCCACGT TGGAGGCAGA GACATTAATG
    AAAGATGATG AAATCTGTGG AACTAAAAAC CCAACTCCCC ATAAAATTTT GCTTGATACA
    AGATTTGAGT TGCCTTTTGA TATCCCAGAA GAGGAAGCTA GATATTGGAC CTACAGATTG
    GAGCAAATGA ATGCCTTGGG ACCTGACAAT GAGTATTCTA GTCAAGAAGA AAGCCAGAGG
    AAGCAATTGC CTACTGCTGC CGCCCAGTGT TCTTTTGAAG ATCCTGACAG TGCCGTGGAT
    GACCGAGATA GTGATTATCG CAGTGAGACC AGCAATAGCA TCCCGCCTCC TTACCATACA
    ACTGCCCAGC CCAACGCTTC TGTGCACCAG TTCCCAGTGC CTGTGCGATT GCCACAGCAG
    CTGCTGCTTC AGGGCAGTTC CCGGGACTCT TGCAATGACT CTGTGCAAAG TTATGACCTT
    GAGTATCCAG AGCGGCGGGC CCTCAGCCCC ACCAGCAGTA GTAGGTATGG CTCCTCCTGT
    AACGTGAGTC AAGGAAGCTC TCAGTTAAGT GAACTGGACC AGTGTCATGA ACAAGATGAT
    GACCGTCGGG AGAGGGACTC CATTCATTCC TGCCACAGCT CCAGCAGCCT CTCCAGGGAT
    GGCCAAGTGG GTTTTGGAGA ACAAGAGAAA GCCTTGGAGG TGACAGGTGA AGAGGAGAAG
    AGGGGAGTGT GTGAACCCAA GGAGATGAAA GAAGATGCCA TGGCCCATCC ACCCCCAGAT
    ATGGTGCTAC ACAAAGACCT TGTCCGAAGC CCCCAGGAGA GTTTTCCTGA GGAGACTGCA
    TCTTCGCCAT TTACCCAAGC CAGAGCGCAC TGGATCCGAG CAGTTACCAA GGTTCGACTC
    CAGCTGCAGG AGAAGGAAAG CGAGGAGCCC TTCAGGTTGT GCTCTGGCCC TTTGGGGCCC
    CTTTGTTGTT GGTTCTCTCC CCTCCCTCCT GAGTACAATA TTCCAGATGG TGGTGATCCC
    TGTATGCCCC AGTGGCTTCC AGAAGGGCCA GCTGGAGGGC TCTATGGCAT TGACAGCATG
    CCGGATCTAC GCAGAAAGAA GCCACTGCCG CTTGTCAGCG ACCTGGCTAT GTCACTGGTC
    CAGTCCCGGA AAGCAGGCAT TACTTCTGCA ATGGCTACAC GCACCTCTCT CAAAGATGAA
    GAGCTGAAAT CACATGTGTA TAAGAAAACC CTGCAGGCCT TAATCTATCC CATCTCGTGC
    ACCACACCCC ACAACTTTGA GGTGTGGTCA GCCACCACCC CCACCTACTG CTATGAGTGT
    GAAGGCCTGC TCTGGGGCAT TGCCCGGCAG GGCATGCGCT GCAGCGAGTG TGGAGTCAAG
    TGCCATGAGA AGTGCCAGGA CCTACTCAAT GCTGACTGCC TGCAGCGGGC TGCAGAAAAG
    AGCTCTAAAC ATGGAGCGGA GGACCGGACC CAGAACATCA TCATGGCCAT GAAGGACCGC
    ATGAAGATCC GAGAGCGGAA TAAGCCAGAG ATCTTTGAAG TTATCCGGGA TGTCTTCAAT
    GTGAGCAAAG TCGCCCATGT GCAACAAATG AAAACAGTGA AGCAGAGTGT GTTGGATGGC
    ACCTCCAAAT GGTCAGCCAA AATCACCATC ACTGTGGTGT GTGCCCAGGG CCTACAAGCC
    AAGGACAAGA CAGGATCCAG TGACCCTTAT GTGACTGTGC AAGTGGGCAA AACCAAGAAG
    CGTACCAAGA CCATTTTTGG AAACCTGAAT CCTGTTTGGG AGGAGAAGTT CCATTTTGAG
    TGCCACAACT CTTCTGACCG CATTAAGGTA CGTGTGTGGG ATGAGGATGA TGACATCAAG
    TCACGAGTAA AGCAGCGGCT AAAGCGAGAG TCTGATGATT TCCTCGGCCA AACCATCATT
    GAGGTTCGAA CACTGAGTGG CGAAATGGAC GTCTGGTACA ACTTGGAGAA GAGGACAGAC
    AAGTCAGCCG TCTCGGGGGC CATCCGACTG CAAATCAGCG TGGAGATCAA GGGAGAAGAG
    AAGGTAGCCC CATATCATGT GCAGTACACG TGTCTCCATG AGAACCTCTT CCATTACCTC
    ACGGACATTC AGGGCAGCGG AGGAGTCCAG ATCCCTGAGG CTCGAGGAGA TGATGCATGG
    AAGGTGTACT TTGATGAGAC TGCCCAGGAA ATTGTGGATG AATTTGCCAT GCGCTATGGC
    ATTGAGTCCA TATATCAGGC CATGACGCAT TTTGCATGTT TGTCATCCAA GTACATGTGT
    CCTGGTGTGC CAGCAGTGAT GAGCACCTTA CTGGCCAACA TCAATGCCTA CTATGCCCAC
    ACAACTGCCT CCACCAATGT CTCTGCATCT GATCGTTTTG CAGCCTCCAA CTTTGGGAAA
    GAGAGATTTG TGAAACTTCT GGACCAGCTG CACAACTCAC TGAGGATTGA CCTCTCTACC
    TACAGGAATA ATTTCCCTGC TGGGAGCCCT GAGCGACTTC AGGACTTAAA GTCCACAGTG
    GATTTGCTGA CCAGCATTAC TTTCTTCCGA ATGAAGGTGC AAGAACTTCA GAGCCCTCCA
    AGAGCCAGCC AGGTAGTAAA AGATTGTGTG AAGGCCTGTT TGAACTCAAC ATATGAGTAT
    ATCTTCAACA ACTGCCATGA CTTGTACAGC CGCCAGTACC AGCTGAATCA GGAGCTGCCT
    CCAGAAGAAC AGGGGCCCAG CATTAGGAAC CTGGATTTCT GGCCCAAACT CATCACACTC
    ATTGTGTCAA TCATAGAGGA AGATAAGAAT TCCTACACCC TCGTTCTGAA CCAGTTTCCC
    CAGGAGTTGA ATGTGGGAAA AGTCAGCGCA GAAGTGATGT GGCAACTCTT TGCCCAAGAC
    ATGAAATATG CACTGGAGGA GCATGAGAAA GACAGGCTGT GTAAGAGTGC TGACTACATG
    AACCTGCACT TCAAGGTGAA GTGGCTCCAC AATGAATACG TGCAGGATCT GCCTGCCCTC
    CAGGGCCAGG TGCCCGAATA CCCAGCGTGG TTTGAGCAGT TTGTCCTGCA GTGGCTGGAC
    GAGAATGAGG ATGTGTCCTT GGAATTCCTG CGTGGGGCCT TGGAACGAGA TAAGAAGGAT
    GGGTTCCAGC AGACATCAGA GCACGCGCTC TTCTCTTGCT CTGTGGTGGA CGTCTTCACA
    CAGCTCAATC AGAGCTTTGA GATCATCCGG AAATTGGAAT GCCCAGACCC CAACATCCTT
    GCCCACTACA TGAGGAGATT TTCTAAGACC ATCGGGAAGG TGCTCATGGA ATATGCAGAC
    ATCTTATCAA AGAACTTCCC AGCTTACTGC ACAAAGGAGA AAATGCCTTG CATCCTGATG
    AACAACATGC AGCAGCTGAG GGTGCAGCTG GAGAAGATGT TTGAGGCCAT GGGAGGCAAG
    GAGCTGGACC CTGAAACTGC AGACAGTCTG AAGGAGCTTC AGGTGAAACT GAATACAGTT
    TTGGATGAGC TCAGCATGGT GTTTGGAAAC AGTTTCCAGG TGCGGATTGA TGAGTGCGTG
    CGACAAATGG CCGACATCCT GAGCCAGGTG CGGGGCCCAG GGAACGCATC CCCCAACGCA
    CGGGCCTCGG TGACTCAGGA TGCAGACAGT GTGCTCCGGC CTCTCATGGA CTTCCTGGAC
    GGCAACCTCA CACTTTTTGC CACTGTGTGT GAGAAGACGG TCCTGAAGCG GGTACTGAAG
    GAACTCTGGC GGGTGGTAAT GAACACAATG GAAAGGATGA TTGTTCTGCC TCCACTTACT
    GACCAGACGG GCACCCAGCT GATCTTCACT GCTGCCAAGG AGCTGAGCCA ACTTTCCAAA
    CTCAAGGACC ACATGGTGCG AGAGGAAACA CGGAATCTGA CTCCAAAGCA GTGTGCCGTC
    CTTGACCTCG CCCTGGACAC CATCAAGCAA TACTTTCACG CCGGAGGCAA CGGGCTGAAG
    AAAACCTTCC TGGAGAAGAG CCCAGATCTG CAGTCCCTGC GCTATGCCCT ATCTCTGTAC
    ACACAGACCA CAGACACCCT CATCAAGACC TTTGTGCGCT CACAGACTGC CCAGGTGCAT
    GATGGGAAAG GTATTAGGTT TACTGCTAAT GAGGACATTC AGCCGGAAAA GGGGTCCGGT
    GTGGATGATC CTGTGGGAGA AGTCTCTATT CAGGTGGACT TGTTTACACA TCCTGGAACT
    GGGGAGCACA AGGTCACAGT GAAAGTGGTG GCTGCCAATG ACCTCAAGTG GCAGACAGCA
    GGTATGTTCC GGCCCTTTGT CGAAGTCACC ATGGTTGGCC CTCACCAAAG TGATAAAAAG
    AGGAAGTTCA CAACCAAGTC AAAAAGTAAC AACTGGGCTC CCAAGTACAA CGAGACGTTT
    CACTTCCTCC TGGGAAATGA AGAGGGGCCA GAAGCCTATG AGTTGCAGAT ATGCGTGAAG
    GATTACTGCT TTGCCCGGGA GGACCGTGTG CTGGGGCTGG CTGTAATGCC TCTGAGGGAT
    GTGGCAGCCA AGGGCAGCTG TGCTTGCTGG TGCCCCTTGG GCCGTAAGAT CCACATGGAT
    GAGACAGGCG TGACCATTCT CCGGATTCTG TCTCAGAGGA GCAATGACGA AGTGGCCCGG
    GAGTTTGTGA AGCTCAAATC AGAGTCTCGT TCTATGGAGG AGGGGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91275
    Clone ID Related Accession (Same CDS sequence) XM_015472526.1
    Accession Version XM_015472526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGGCTATGT CACTGGTCCA GTCCCGGAAA
    GCAGGCATTA CTTCTGCAAT GGCTACACGC ACCTCTCTCA AAGATGAAGA GCTGAAATCA
    CATGTGTATA AGAAAACCCT GCAGGCCTTA ATCTATCCCA TCTCGTGCAC CACACCCCAC
    AACTTTGAGG TGTGGTCAGC CACCACCCCC ACCTACTGCT ATGAGTGTGA AGGCCTGCTC
    TGGGGCATTG CCCGGCAGGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG
    TGCCAGGACC TACTCAATGC TGACTGCCTG CAGCGGGCTG CAGAAAAGAG CTCTAAACAT
    GGAGCGGAGG ACCGGACCCA GAACATCATC ATGGCCATGA AGGACCGCAT GAAGATCCGA
    GAGCGGAATA AGCCAGAGAT CTTTGAAGTT ATCCGGGATG TCTTCAATGT GAGCAAAGTC
    GCCCATGTGC AACAAATGAA AACAGTGAAG CAGAGTGTGT TGGATGGCAC CTCCAAATGG
    TCAGCCAAAA TCACCATCAC TGTGGTGTGT GCCCAGGGCC TACAAGCCAA GGACAAGACA
    GGATCCAGTG ACCCTTATGT GACTGTGCAA GTGGGCAAAA CCAAGAAGCG TACCAAGACC
    ATTTTTGGAA ACCTGAATCC TGTTTGGGAG GAGAAGTTCC ATTTTGAGTG CCACAACTCT
    TCTGACCGCA TTAAGGTACG TGTGTGGGAT GAGGATGATG ACATCAAGTC ACGAGTAAAG
    CAGCGGCTAA AGCGAGAGTC TGATGATTTC CTCGGCCAAA CCATCATTGA GGTTCGAACA
    CTGAGTGGCG AAATGGACGT CTGGTACAAC TTGGAGAAGA GGACAGACAA GTCAGCCGTC
    TCGGGGGCCA TCCGACTGCA AATCAGCGTG GAGATCAAGG GAGAAGAGAA GGTAGCCCCA
    TATCATGTGC AGTACACGTG TCTCCATGAG AACCTCTTCC ATTACCTCAC GGACATTCAG
    GGCAGCGGAG GAGTCCAGAT CCCTGAGGCT CGAGGAGATG ATGCATGGAA GGTGTACTTT
    GATGAGACTG CCCAGGAAAT TGTGGATGAA TTTGCCATGC GCTATGGCAT TGAGTCCATA
    TATCAGGCCA TGACGCATTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTGCCA
    GCAGTGATGA GCACCTTACT GGCCAACATC AATGCCTACT ATGCCCACAC AACTGCCTCC
    ACCAATGTCT CTGCATCTGA TCGTTTTGCA GCCTCCAACT TTGGGAAAGA GAGATTTGTG
    AAACTTCTGG ACCAGCTGCA CAACTCACTG AGGATTGACC TCTCTACCTA CAGGAATAAT
    TTCCCTGCTG GGAGCCCTGA GCGACTTCAG GACTTAAAGT CCACAGTGGA TTTGCTGACC
    AGCATTACTT TCTTCCGAAT GAAGGTGCAA GAACTTCAGA GCCCTCCAAG AGCCAGCCAG
    GTAGTAAAAG ATTGTGTGAA GGCCTGTTTG AACTCAACAT ATGAGTATAT CTTCAACAAC
    TGCCATGACT TGTACAGCCG CCAGTACCAG CTGAATCAGG AGCTGCCTCC AGAAGAACAG
    GGGCCCAGCA TTAGGAACCT GGATTTCTGG CCCAAACTCA TCACACTCAT TGTGTCAATC
    ATAGAGGAAG ATAAGAATTC CTACACCCTC GTTCTGAACC AGTTTCCCCA GGAGTTGAAT
    GTGGGAAAAG TCAGCGCAGA AGTGATGTGG CAACTCTTTG CCCAAGACAT GAAATATGCA
    CTGGAGGAGC ATGAGAAAGA CAGGCTGTGT AAGAGTGCTG ACTACATGAA CCTGCACTTC
    AAGGTGAAGT GGCTCCACAA TGAATACGTG CAGGATCTGC CTGCCCTCCA GGGCCAGGTG
    CCCGAATACC CAGCGTGGTT TGAGCAGTTT GTCCTGCAGT GGCTGGACGA GAATGAGGAT
    GTGTCCTTGG AATTCCTGCG TGGGGCCTTG GAACGAGATA AGAAGGATGG GTTCCAGCAG
    ACATCAGAGC ACGCGCTCTT CTCTTGCTCT GTGGTGGACG TCTTCACACA GCTCAATCAG
    AGCTTTGAGA TCATCCGGAA ATTGGAATGC CCAGACCCCA ACATCCTTGC CCACTACATG
    AGGAGATTTT CTAAGACCAT CGGGAAGGTG CTCATGGAAT ATGCAGACAT CTTATCAAAG
    AACTTCCCAG CTTACTGCAC AAAGGAGAAA ATGCCTTGCA TCCTGATGAA CAACATGCAG
    CAGCTGAGGG TGCAGCTGGA GAAGATGTTT GAGGCCATGG GAGGCAAGGA GCTGGACCCT
    GAAACTGCAG ACAGTCTGAA GGAGCTTCAG GTGAAACTGA ATACAGTTTT GGATGAGCTC
    AGCATGGTGT TTGGAAACAG TTTCCAGGTG CGGATTGATG AGTGCGTGCG ACAAATGGCC
    GACATCCTGA GCCAGGTGCG GGGCCCAGGG AACGCATCCC CCAACGCACG GGCCTCGGTG
    ACTCAGGATG CAGACAGTGT GCTCCGGCCT CTCATGGACT TCCTGGACGG CAACCTCACA
    CTTTTTGCCA CTGTGTGTGA GAAGACGGTC CTGAAGCGGG TACTGAAGGA ACTCTGGCGG
    GTGGTAATGA ACACAATGGA AAGGATGATT GTTCTGCCTC CACTTACTGA CCAGACGGGC
    ACCCAGCTGA TCTTCACTGC TGCCAAGGAG CTGAGCCAAC TTTCCAAACT CAAGGACCAC
    ATGGTGCGAG AGGAAACACG GAATCTGACT CCAAAGCAGT GTGCCGTCCT TGACCTCGCC
    CTGGACACCA TCAAGCAATA CTTTCACGCC GGAGGCAACG GGCTGAAGAA AACCTTCCTG
    GAGAAGAGCC CAGATCTGCA GTCCCTGCGC TATGCCCTAT CTCTGTACAC ACAGACCACA
    GACACCCTCA TCAAGACCTT TGTGCGCTCA CAGACTGCCC AGGTGCATGA TGGGAAAGGT
    ATTAGGTTTA CTGCTAATGA GGACATTCAG CCGGAAAAGG GGTCCGGTGT GGATGATCCT
    GTGGGAGAAG TCTCTATTCA GGTGGACTTG TTTACACATC CTGGAACTGG GGAGCACAAG
    GTCACAGTGA AAGTGGTGGC TGCCAATGAC CTCAAGTGGC AGACAGCAGG TATGTTCCGG
    CCCTTTGTCG AAGTCACCAT GGTTGGCCCT CACCAAAGTG ATAAAAAGAG GAAGTTCACA
    ACCAAGTCAA AAAGTAACAA CTGGGCTCCC AAGTACAACG AGACGTTTCA CTTCCTCCTG
    GGAAATGAAG AGGGGCCAGA AGCCTATGAG TTGCAGATAT GCGTGAAGGA TTACTGCTTT
    GCCCGGGAGG ACCGTGTGCT GGGGCTGGCT GTAATGCCTC TGAGGGATGT GGCAGCCAAG
    GGCAGCTGTG CTTGCTGGTG CCCCTTGGGC CGTAAGATCC ACATGGATGA GACAGGCGTG
    ACCATTCTCC GGATTCTGTC TCAGAGGAGC AATGACGAAG TGGCCCGGGA GTTTGTGAAG
    CTCAAATCAG AGTCTCGTTC TATGGAGGAG GGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328012.1
    CDS331..5169
    Translation

    Target ORF information:

    RefSeq Version XM_015472526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGGCTATGT CACTGGTCCA GTCCCGGAAA
    GCAGGCATTA CTTCTGCAAT GGCTACACGC ACCTCTCTCA AAGATGAAGA GCTGAAATCA
    CATGTGTATA AGAAAACCCT GCAGGCCTTA ATCTATCCCA TCTCGTGCAC CACACCCCAC
    AACTTTGAGG TGTGGTCAGC CACCACCCCC ACCTACTGCT ATGAGTGTGA AGGCCTGCTC
    TGGGGCATTG CCCGGCAGGG CATGCGCTGC AGCGAGTGTG GAGTCAAGTG CCATGAGAAG
    TGCCAGGACC TACTCAATGC TGACTGCCTG CAGCGGGCTG CAGAAAAGAG CTCTAAACAT
    GGAGCGGAGG ACCGGACCCA GAACATCATC ATGGCCATGA AGGACCGCAT GAAGATCCGA
    GAGCGGAATA AGCCAGAGAT CTTTGAAGTT ATCCGGGATG TCTTCAATGT GAGCAAAGTC
    GCCCATGTGC AACAAATGAA AACAGTGAAG CAGAGTGTGT TGGATGGCAC CTCCAAATGG
    TCAGCCAAAA TCACCATCAC TGTGGTGTGT GCCCAGGGCC TACAAGCCAA GGACAAGACA
    GGATCCAGTG ACCCTTATGT GACTGTGCAA GTGGGCAAAA CCAAGAAGCG TACCAAGACC
    ATTTTTGGAA ACCTGAATCC TGTTTGGGAG GAGAAGTTCC ATTTTGAGTG CCACAACTCT
    TCTGACCGCA TTAAGGTACG TGTGTGGGAT GAGGATGATG ACATCAAGTC ACGAGTAAAG
    CAGCGGCTAA AGCGAGAGTC TGATGATTTC CTCGGCCAAA CCATCATTGA GGTTCGAACA
    CTGAGTGGCG AAATGGACGT CTGGTACAAC TTGGAGAAGA GGACAGACAA GTCAGCCGTC
    TCGGGGGCCA TCCGACTGCA AATCAGCGTG GAGATCAAGG GAGAAGAGAA GGTAGCCCCA
    TATCATGTGC AGTACACGTG TCTCCATGAG AACCTCTTCC ATTACCTCAC GGACATTCAG
    GGCAGCGGAG GAGTCCAGAT CCCTGAGGCT CGAGGAGATG ATGCATGGAA GGTGTACTTT
    GATGAGACTG CCCAGGAAAT TGTGGATGAA TTTGCCATGC GCTATGGCAT TGAGTCCATA
    TATCAGGCCA TGACGCATTT TGCATGTTTG TCATCCAAGT ACATGTGTCC TGGTGTGCCA
    GCAGTGATGA GCACCTTACT GGCCAACATC AATGCCTACT ATGCCCACAC AACTGCCTCC
    ACCAATGTCT CTGCATCTGA TCGTTTTGCA GCCTCCAACT TTGGGAAAGA GAGATTTGTG
    AAACTTCTGG ACCAGCTGCA CAACTCACTG AGGATTGACC TCTCTACCTA CAGGAATAAT
    TTCCCTGCTG GGAGCCCTGA GCGACTTCAG GACTTAAAGT CCACAGTGGA TTTGCTGACC
    AGCATTACTT TCTTCCGAAT GAAGGTGCAA GAACTTCAGA GCCCTCCAAG AGCCAGCCAG
    GTAGTAAAAG ATTGTGTGAA GGCCTGTTTG AACTCAACAT ATGAGTATAT CTTCAACAAC
    TGCCATGACT TGTACAGCCG CCAGTACCAG CTGAATCAGG AGCTGCCTCC AGAAGAACAG
    GGGCCCAGCA TTAGGAACCT GGATTTCTGG CCCAAACTCA TCACACTCAT TGTGTCAATC
    ATAGAGGAAG ATAAGAATTC CTACACCCTC GTTCTGAACC AGTTTCCCCA GGAGTTGAAT
    GTGGGAAAAG TCAGCGCAGA AGTGATGTGG CAACTCTTTG CCCAAGACAT GAAATATGCA
    CTGGAGGAGC ATGAGAAAGA CAGGCTGTGT AAGAGTGCTG ACTACATGAA CCTGCACTTC
    AAGGTGAAGT GGCTCCACAA TGAATACGTG CAGGATCTGC CTGCCCTCCA GGGCCAGGTG
    CCCGAATACC CAGCGTGGTT TGAGCAGTTT GTCCTGCAGT GGCTGGACGA GAATGAGGAT
    GTGTCCTTGG AATTCCTGCG TGGGGCCTTG GAACGAGATA AGAAGGATGG GTTCCAGCAG
    ACATCAGAGC ACGCGCTCTT CTCTTGCTCT GTGGTGGACG TCTTCACACA GCTCAATCAG
    AGCTTTGAGA TCATCCGGAA ATTGGAATGC CCAGACCCCA ACATCCTTGC CCACTACATG
    AGGAGATTTT CTAAGACCAT CGGGAAGGTG CTCATGGAAT ATGCAGACAT CTTATCAAAG
    AACTTCCCAG CTTACTGCAC AAAGGAGAAA ATGCCTTGCA TCCTGATGAA CAACATGCAG
    CAGCTGAGGG TGCAGCTGGA GAAGATGTTT GAGGCCATGG GAGGCAAGGA GCTGGACCCT
    GAAACTGCAG ACAGTCTGAA GGAGCTTCAG GTGAAACTGA ATACAGTTTT GGATGAGCTC
    AGCATGGTGT TTGGAAACAG TTTCCAGGTG CGGATTGATG AGTGCGTGCG ACAAATGGCC
    GACATCCTGA GCCAGGTGCG GGGCCCAGGG AACGCATCCC CCAACGCACG GGCCTCGGTG
    ACTCAGGATG CAGACAGTGT GCTCCGGCCT CTCATGGACT TCCTGGACGG CAACCTCACA
    CTTTTTGCCA CTGTGTGTGA GAAGACGGTC CTGAAGCGGG TACTGAAGGA ACTCTGGCGG
    GTGGTAATGA ACACAATGGA AAGGATGATT GTTCTGCCTC CACTTACTGA CCAGACGGGC
    ACCCAGCTGA TCTTCACTGC TGCCAAGGAG CTGAGCCAAC TTTCCAAACT CAAGGACCAC
    ATGGTGCGAG AGGAAACACG GAATCTGACT CCAAAGCAGT GTGCCGTCCT TGACCTCGCC
    CTGGACACCA TCAAGCAATA CTTTCACGCC GGAGGCAACG GGCTGAAGAA AACCTTCCTG
    GAGAAGAGCC CAGATCTGCA GTCCCTGCGC TATGCCCTAT CTCTGTACAC ACAGACCACA
    GACACCCTCA TCAAGACCTT TGTGCGCTCA CAGACTGCCC AGGTGCATGA TGGGAAAGGT
    ATTAGGTTTA CTGCTAATGA GGACATTCAG CCGGAAAAGG GGTCCGGTGT GGATGATCCT
    GTGGGAGAAG TCTCTATTCA GGTGGACTTG TTTACACATC CTGGAACTGG GGAGCACAAG
    GTCACAGTGA AAGTGGTGGC TGCCAATGAC CTCAAGTGGC AGACAGCAGG TATGTTCCGG
    CCCTTTGTCG AAGTCACCAT GGTTGGCCCT CACCAAAGTG ATAAAAAGAG GAAGTTCACA
    ACCAAGTCAA AAAGTAACAA CTGGGCTCCC AAGTACAACG AGACGTTTCA CTTCCTCCTG
    GGAAATGAAG AGGGGCCAGA AGCCTATGAG TTGCAGATAT GCGTGAAGGA TTACTGCTTT
    GCCCGGGAGG ACCGTGTGCT GGGGCTGGCT GTAATGCCTC TGAGGGATGT GGCAGCCAAG
    GGCAGCTGTG CTTGCTGGTG CCCCTTGGGC CGTAAGATCC ACATGGATGA GACAGGCGTG
    ACCATTCTCC GGATTCTGTC TCAGAGGAGC AATGACGAAG TGGCCCGGGA GTTTGTGAAG
    CTCAAATCAG AGTCTCGTTC TATGGAGGAG GGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01673
    Clone ID Related Accession (Same CDS sequence) NM_001193239.1
    Accession Version NM_001193239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(Cattle)
    Product protein unc-13 homolog B
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03001375.1, AAFC03123148.1, AAFC03001376.1, AAFC03018168.1 and AAFC03018169.1. On Jul 17, 2010 this sequence version replaced XM_877667.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC TCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC GTGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180168.1
    CDS336..5111
    Translation

    Target ORF information:

    RefSeq Version NM_001193239.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC TCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGGGT CCGGTGTGGA TGATCCTGTG
    GGAGAAGTCT CTATTCAGGT GGACTTGTTT ACACATCCTG GAACTGGGGA GCACAAGGTC
    ACAGTGAAAG TGGTGGCTGC CAATGACCTC AAGTGGCAGA CAGCAGGTAT GTTCCGGCCC
    TTTGTCGAAG TCACCATGGT TGGCCCTCAC CAAAGTGATA AAAAGAGGAA GTTCACAACC
    AAGTCAAAAA GTAACAACTG GGCTCCCAAG TACAACGAGA CGTTTCACTT CCTCCTGGGA
    AATGAAGAGG GGCCAGAAGC CTATGAGTTG CAGATATGCG TGAAGGATTA CTGCTTTGCC
    CGGGAGGACC GTGTGCTGGG GCTGGCTGTA ATGCCTCTGA GGGATGTGGC AGCCAAGGGC
    AGCTGTGCTT GCTGGTGCCC CTTGGGCCGT AAGATCCACA TGGATGAGAC AGGCGTGACC
    ATTCTCCGGA TTCTGTCTCA GAGGAGCAAT GACGAAGTGG CCCGGGAGTT TGTGAAGCTC
    AAATCAGAGT CTCGTTCTAT GGAGGAGGGG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22042
    Clone ID Related Accession (Same CDS sequence) XM_015472522.1
    Accession Version XM_015472522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GAGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGACAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA
    GTCAGCGCAG AAGTGATGTG GCAACTCTTT GCCCAAGACA TGAAATATGC ACTGGAGGAG
    CATGAGAAAG ACAGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG
    TGGCTCCACA ATGAATACGT GCAGGATCTG CCTGCCCTCC AGGGCCAGGT GCCCGAATAC
    CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG TGGCTGGACG AGAATGAGGA TGTGTCCTTG
    GAATTCCTGC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG
    CACGCGCTCT TCTCTTGCTC TGTGGTGGAC GTCTTCACAC AGCTCAATCA GAGCTTTGAG
    ATCATCCGGA AATTGGAATG CCCAGACCCC AACATCCTTG CCCACTACAT GAGGAGATTT
    TCTAAGACCA TCGGGAAGGT GCTCATGGAA TATGCAGACA TCTTATCAAA GAACTTCCCA
    GCTTACTGCA CAAAGGAGAA AATGCCTTGC ATCCTGATGA ACAACATGCA GCAGCTGAGG
    GTGCAGCTGG AGAAGATGTT TGAGGCCATG GGAGGCAAGG AGCTGGACCC TGAAACTGCA
    GACAGTCTGA AGGAGCTTCA GGTGAAACTG AATACAGTTT TGGATGAGCT CAGCATGGTG
    TTTGGAAACA GTTTCCAGGT GCGGATTGAT GAGTGCGTGC GACAAATGGC CGACATCCTG
    AGCCAGGTGC GGGGCCCAGG GAACGCATCC CCCAACGCAC GGGCCTCGGT GACTCAGGAT
    GCAGACAGTG TGCTCCGGCC TCTCATGGAC TTCCTGGACG GCAACCTCAC ACTTTTTGCC
    ACTGTGTGTG AGAAGACGGT CCTGAAGCGG GTACTGAAGG AACTCTGGCG GGTGGTAATG
    AACACAATGG AAAGGATGAT TGTTCTGCCT CCACTTACTG ACCAGACGGG CACCCAGCTG
    ATCTTCACTG CTGCCAAGGA GCTGAGCCAA CTTTCCAAAC TCAAGGACCA CATGGTGCGA
    GAGGAAACAC GGAATCTGAC TCCAAAGCAG TGTGCCGTCC TTGACCTCGC CCTGGACACC
    ATCAAGCAAT ACTTTCACGC CGGAGGCAAC GGGCTGAAGA AAACCTTCCT GGAGAAGAGC
    CCAGATCTGC AGTCCCTGCG CTATGCCCTA TCTCTGTACA CACAGACCAC AGACACCCTC
    ATCAAGACCT TTGTGCGCTC ACAGACTGCC CAGGTGCATG ATGGGAAAGG TATTAGGTTT
    ACTGCTAATG AGGACATTCA GCCGGAAAAG GGGTCCGGTG TGGATGATCC TGTGGGAGAA
    GTCTCTATTC AGGTGGACTT GTTTACACAT CCTGGAACTG GGGAGCACAA GGTCACAGTG
    AAAGTGGTGG CTGCCAATGA CCTCAAGTGG CAGACAGCAG GTATGTTCCG GCCCTTTGTC
    GAAGTCACCA TGGTTGGCCC TCACCAAAGT GATAAAAAGA GGAAGTTCAC AACCAAGTCA
    AAAAGTAACA ACTGGGCTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA
    GAGGGGCCAG AAGCCTATGA GTTGCAGATA TGCGTGAAGG ATTACTGCTT TGCCCGGGAG
    GACCGTGTGC TGGGGCTGGC TGTAATGCCT CTGAGGGATG TGGCAGCCAA GGGCAGCTGT
    GCTTGCTGGT GCCCCTTGGG CCGTAAGATC CACATGGATG AGACAGGCGT GACCATTCTC
    CGGATTCTGT CTCAGAGGAG CAATGACGAA GTGGCCCGGG AGTTTGTGAA GCTCAAATCA
    GAGTCTCGTT CTATGGAGGA GGGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328008.1
    CDS378..6347
    Translation

    Target ORF information:

    RefSeq Version XM_015472522.1
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGAAGCGAT TGTTGCAAGA ATCAGAGGAA GAAATCATGG TCACCTTGGG ATCCTCCTCC 
    AGGCTTTCCC CAGATAAAGC TAAGGCAGAG ACTCTGTGTG GCATCAAGAG CAAGTCCTCA
    GGCCCTGCTG GAACCTTGCC AGAGGACAGC TTCCTGCCCC CTCACTGTGG GCCTGTAGCC
    TCCGCCATTG GTGGGGATAC AGATGGGGGT CTGGCCCAGC ATTGCAGCTT TGGGCAGCAG
    GCCAGCAGCC AGCTTCCTCT GAGCTCCACA GCCTGTGTTT CAGGGTCTGC CTCTCAGGAC
    CTCTCATCTG CCAGCATTAC CAGAAGCTGT CCAGAGCCCT CAGAGAGGGA TCAAGCCAAG
    CCCCTTTTGT CCAGAGGCCT TGGCCAAGGC TGTCGCCATG AACAGCAAGA ACCTTTGGGG
    GATGTAGTGG ACTATATCAT CAGAGAACTG CAGGGCATCA GCCGTCTCCA AACTGAGATT
    GCTGAGTTGC GGCAGCACTT GAGCCAAGTC CGAGGTTCGG TGGATGAGGT CTCTAGCTGT
    GTAGACTCAG TTCTGAGTGA AATAGAAGGC TTACATGTGG GCAGCAGTTC CCTGGCCAAA
    GGGTGTGTGG GGGGAAAGGC CCAGGAACCC CATGTGGACA GACCCAGCGA GGAAGCCATT
    CTGTATCTGT ATGGACTTCC TGAGCAAGAT GGGGAAAATA CCATGGAGCT GGCTCACAAC
    TTCCTGGCCA AGCACATGTG TGTTAATGGC ATGCAGTGCA ATAGGTTCAT TAAAGAGGCT
    TACCGAGCTG GCAGAGCCCC AGCTCCCAGG CCTACTGTTG TGAAGCTTGC CCATCTAGAG
    CATAGGGACC TCATATTGCA GAAATCCATC CTCTTGCAGA GTTTAGGGGT CCGGATCACC
    ACCAGAGAAG AGCCAAGCCA GCCACCATGC TCTAAGAATC CCCCAAAGGA GTCTCTCTCA
    TCTTTGGAAC AACAACTCCA GCATCGTAAT TTGAATTCTT TACACCTGGA TCAGCCAGTT
    TTTCAGGTGG AAACGGGGGA CAGAGGACCA ATAACAGGAG CACATCAAAT GAGGGCTCAG
    AATCAACATA GAGAACCCCA GACCCCTGAA CAGCAAGGCC TTTGCTTCCC ACCCAAGAAT
    GATTTGGCAA AGCCAAGTAA TGAGTCTAAG CAAGGGGGTG AAGTCAGAGG AACATTAGGA
    AACCCCCAGG TTCTCAGGGG CTCCTGCAGT GAATTGACAG GAAGAGAGGC TTCTTTATCC
    ACCCTCCATC AATGTGAGGA ACCTTCCCTA GTGTTAATCT CGAAAGAGGA GGATTCTGGG
    AAATCCCAGG TTTTCAAACA GTGTAACCAA GGACTTGAAG TATATAAAAC AGCAAAAACA
    GAAAACAACT GCAGCGACAA AAGTGAGACT GGCAGTTGCC TATCACTGTC TGGGTTGCTG
    AAGACAGAGG ACAAACTCCT GGCCTGTGAA CCTGGGTTGG ATATTCTGAG CGCAAAGCAG
    CTAGAGGACC TCTTAACAGA TAAGTCCAGA AGGTTTGCAA CTCTGAGCTG TGACAGCCTG
    ATGGAGGAAA TCAGCATTGG GCCCGAGACT TTCAATGACA TGGTTCATAT TGACTTGAAT
    GAGGAGGAGG AACATTCTGC TCAGGTCCTC AAAGATGTCT TGGAAAAGTC CTCTCGCCTG
    GGTGGCTCGC AGGAGGATGA AGATGTAGAG ATCAAGTTTT ACACGAGTAA ACTGGGCCGA
    GCAATTGACC ACTTTCGTTC AGCTTTGCAG GGAGTGTTTC AGAAGCTGGA GAACAGTGGG
    TCCATCACCC CTGAGGACCT TGAAAGCATT GAGAGTGGCT CCCAGTCCGA GAACAGTGAT
    AGACTTCTTG GCACAGTGAG TTCAGGGGGT GCTCAGGATT TCTCACTGGG GAGCCCTGGG
    AGTCAGGGGA GTGAGAGCAT ACTCAGCGTG GTGTCTGGTG GAGTGGGCAT CTCTGCCCAG
    GGAGACCAGA CCTCTCAGGA TCCCAGCAAC TCCTCTCTGG CTTCCAGTAA CTTGCCTCTT
    GCATATTCAT CGCCAGGGTT TGCTCTGGCT CCGTGTCTGG GAAGTGAAAC TTGTTCCAGG
    CCTGAATCTC CCAAACAGGG CAGACTAAGC CTAGAGCAAG TGTGCACAGA GACAATCTAC
    CTCAACAGAT GCATCAGCAA TTTTAAAGAT GTTCTAAGAG AGAAAAGACT GAGGCAGAAA
    AAACTTATAC ACGAGCTAGT ACAGAAGACA GACCATCTCT CAGTAGAAGA CACGCACCCA
    GAAGGAAAGC GAGGAGCCCT TCAGATTCCA GATGGTGGTG ATCCCTGTAT GCCCCAGTGG
    CTTCCAGAAG GGCCAGCTGG AGGGCTCTAT GGCATTGACA GCATGCCGGA TCTACGCAGA
    AAGAAGCCAC TGCCGCTTGT CAGCGACCTG TCACTGGTCC AGTCCCGGAA AGCAGGCATT
    ACTTCTGCAA TGGCTACACG CACCTCTCTC AAAGATGAAG AGCTGAAATC ACATGTGTAT
    AAGAAAACCC TGCAGGCCTT AATCTATCCC ATCTCGTGCA CCACACCCCA CAACTTTGAG
    GTGTGGTCAG CCACCACCCC CACCTACTGC TATGAGTGTG AAGGCCTGCT CTGGGGCATT
    GCCCGGCAGG GCATGCGCTG CAGCGAGTGT GGAGTCAAGT GCCATGAGAA GTGCCAGGAC
    CTACTCAATG CTGACTGCCT GCAGCGGGCT GCAGAAAAGA GCTCTAAACA TGGAGCGGAG
    GACCGGACCC AGAACATCAT CATGGCCATG AAGGACCGCA TGAAGATCCG AGAGCGGAAT
    AAGCCAGAGA TCTTTGAAGT TATCCGGGAT GTCTTCAATG TGAGCAAAGT CGCCCATGTG
    CAACAAATGA AAACAGTGAA GCAGAGTGTG TTGGATGGCA CCTCCAAATG GTCAGCCAAA
    ATCACCATCA CTGTGGTGTG TGCCCAGGGC CTACAAGCCA AGGACAAGAC AGGATCCAGT
    GACCCTTATG TGACTGTGCA AGTGGGCAAA ACCAAGAAGC GTACCAAGAC CATTTTTGGA
    AACCTGAATC CTGTTTGGGA GGAGAAGTTC CATTTTGAGT GCCACAACTC TTCTGACCGC
    ATTAAGGTAC GTGTGTGGGA TGAGGATGAT GACATCAAGT CACGAGTAAA GCAGCGGCTA
    AAGCGAGAGT CTGATGATTT CCTCGGCCAA ACCATCATTG AGGTTCGAAC ACTGAGTGGC
    GAAATGGACG TCTGGTACAA CTTGGAGAAG AGGACAGACA AGTCAGCCGT CTCGGGGGCC
    ATCCGACTGC AAATCAGCGT GGAGATCAAG GGAGAAGAGA AGGTAGCCCC ATATCATGTG
    CAGTACACGT GTCTCCATGA GAACCTCTTC CATTACCTCA CGGACATTCA GGGCAGCGGA
    GGAGTCCAGA TCCCTGAGGC TCGAGGAGAT GATGCATGGA AGGTGTACTT TGATGAGACT
    GCCCAGGAAA TTGTGGATGA ATTTGCCATG CGCTATGGCA TTGAGTCCAT ATATCAGGCC
    ATGACGCATT TTGCATGTTT GTCATCCAAG TACATGTGTC CTGGTGTGCC AGCAGTGATG
    AGCACCTTAC TGGCCAACAT CAATGCCTAC TATGCCCACA CAACTGCCTC CACCAATGTC
    TCTGCATCTG ATCGTTTTGC AGCCTCCAAC TTTGGGAAAG AGAGATTTGT GAAACTTCTG
    GACCAGCTGC ACAACTCACT GAGGATTGAC CTCTCTACCT ACAGGAATAA TTTCCCTGCT
    GGGAGCCCTG AGCGACTTCA GGACTTAAAG TCCACAGTGG ATTTGCTGAC CAGCATTACT
    TTCTTCCGAA TGAAGGTGCA AGAACTTCAG AGCCCTCCAA GAGCCAGCCA GGTAGTAAAA
    GATTGTGTGA AGGCCTGTTT GAACTCAACA TATGAGTATA TCTTCAACAA CTGCCATGAC
    TTGTACAGCC GCCAGTACCA GCTGAATCAG GAGCTGCCTC CAGAAGAACA GGGGCCCAGC
    ATTAGGAACC TGGATTTCTG GCCCAAACTC ATCACACTCA TTGTGTCAAT CATAGAGGAA
    GATAAGAATT CCTACACCCT CGTTCTGAAC CAGTTTCCCC AGGAGTTGAA TGTGGGAAAA
    GTCAGCGCAG AAGTGATGTG GCAACTCTTT GCCCAAGACA TGAAATATGC ACTGGAGGAG
    CATGAGAAAG ACAGGCTGTG TAAGAGTGCT GACTACATGA ACCTGCACTT CAAGGTGAAG
    TGGCTCCACA ATGAATACGT GCAGGATCTG CCTGCCCTCC AGGGCCAGGT GCCCGAATAC
    CCAGCGTGGT TTGAGCAGTT TGTCCTGCAG TGGCTGGACG AGAATGAGGA TGTGTCCTTG
    GAATTCCTGC GTGGGGCCTT GGAACGAGAT AAGAAGGATG GGTTCCAGCA GACATCAGAG
    CACGCGCTCT TCTCTTGCTC TGTGGTGGAC GTCTTCACAC AGCTCAATCA GAGCTTTGAG
    ATCATCCGGA AATTGGAATG CCCAGACCCC AACATCCTTG CCCACTACAT GAGGAGATTT
    TCTAAGACCA TCGGGAAGGT GCTCATGGAA TATGCAGACA TCTTATCAAA GAACTTCCCA
    GCTTACTGCA CAAAGGAGAA AATGCCTTGC ATCCTGATGA ACAACATGCA GCAGCTGAGG
    GTGCAGCTGG AGAAGATGTT TGAGGCCATG GGAGGCAAGG AGCTGGACCC TGAAACTGCA
    GACAGTCTGA AGGAGCTTCA GGTGAAACTG AATACAGTTT TGGATGAGCT CAGCATGGTG
    TTTGGAAACA GTTTCCAGGT GCGGATTGAT GAGTGCGTGC GACAAATGGC CGACATCCTG
    AGCCAGGTGC GGGGCCCAGG GAACGCATCC CCCAACGCAC GGGCCTCGGT GACTCAGGAT
    GCAGACAGTG TGCTCCGGCC TCTCATGGAC TTCCTGGACG GCAACCTCAC ACTTTTTGCC
    ACTGTGTGTG AGAAGACGGT CCTGAAGCGG GTACTGAAGG AACTCTGGCG GGTGGTAATG
    AACACAATGG AAAGGATGAT TGTTCTGCCT CCACTTACTG ACCAGACGGG CACCCAGCTG
    ATCTTCACTG CTGCCAAGGA GCTGAGCCAA CTTTCCAAAC TCAAGGACCA CATGGTGCGA
    GAGGAAACAC GGAATCTGAC TCCAAAGCAG TGTGCCGTCC TTGACCTCGC CCTGGACACC
    ATCAAGCAAT ACTTTCACGC CGGAGGCAAC GGGCTGAAGA AAACCTTCCT GGAGAAGAGC
    CCAGATCTGC AGTCCCTGCG CTATGCCCTA TCTCTGTACA CACAGACCAC AGACACCCTC
    ATCAAGACCT TTGTGCGCTC ACAGACTGCC CAGGTGCATG ATGGGAAAGG TATTAGGTTT
    ACTGCTAATG AGGACATTCA GCCGGAAAAG GGGTCCGGTG TGGATGATCC TGTGGGAGAA
    GTCTCTATTC AGGTGGACTT GTTTACACAT CCTGGAACTG GGGAGCACAA GGTCACAGTG
    AAAGTGGTGG CTGCCAATGA CCTCAAGTGG CAGACAGCAG GTATGTTCCG GCCCTTTGTC
    GAAGTCACCA TGGTTGGCCC TCACCAAAGT GATAAAAAGA GGAAGTTCAC AACCAAGTCA
    AAAAGTAACA ACTGGGCTCC CAAGTACAAC GAGACGTTTC ACTTCCTCCT GGGAAATGAA
    GAGGGGCCAG AAGCCTATGA GTTGCAGATA TGCGTGAAGG ATTACTGCTT TGCCCGGGAG
    GACCGTGTGC TGGGGCTGGC TGTAATGCCT CTGAGGGATG TGGCAGCCAA GGGCAGCTGT
    GCTTGCTGGT GCCCCTTGGG CCGTAAGATC CACATGGATG AGACAGGCGT GACCATTCTC
    CGGATTCTGT CTCAGAGGAG CAATGACGAA GTGGCCCGGG AGTTTGTGAA GCTCAAATCA
    GAGTCTCGTT CTATGGAGGA GGGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22045
    Clone ID Related Accession (Same CDS sequence) XM_005210089.2
    Accession Version XM_005210089.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein unc-13 homolog B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005210089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGTGC ATGATGGGAA AGGTATTAGG
    TTTACTGCTA ATGAGGACAT TCAGCCGGAA AAGGGGTCCG GTGTGGATGA TCCTGTGGGA
    GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA
    GTGAAAGTGG TGGCTGCCAA TGACCTCAAG TGGCAGACAG CAGGTATGTT CCGGCCCTTT
    GTCGAAGTCA CCATGGTTGG CCCTCACCAA AGTGATAAAA AGAGGAAGTT CACAACCAAG
    TCAAAAAGTA ACAACTGGGC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT
    GAAGAGGGGC CAGAAGCCTA TGAGTTGCAG ATATGCGTGA AGGATTACTG CTTTGCCCGG
    GAGGACCGTG TGCTGGGGCT GGCTGTAATG CCTCTGAGGG ATGTGGCAGC CAAGGGCAGC
    TGTGCTTGCT GGTGCCCCTT GGGCCGTAAG ATCCACATGG ATGAGACAGG CGTGACCATT
    CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAAGTGGCCC GGGAGTTTGT GAAGCTCAAA
    TCAGAGTCTC GTTCTATGGA GGAGGGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210146.1
    CDS331..5163
    Translation

    Target ORF information:

    RefSeq Version XM_005210089.2
    Organism Bos taurus(cattle)
    Definition Bos taurus unc-13 homolog B (C. elegans) (UNC13B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210089.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCGCTGC TCTGCGTGCG CGTTAAAAGA GCCAAATTCC AGGGTTCACC AGACAAATTT 
    AACACATATG TGACCCTGAA AGTGCAAAAT GTGAAGAGTA CAACTGTAGC AGTTCGTGGC
    GATCAACCTT CCTGGGAACA AGATTTTATG TTTGAGATTA GTCGCCTGGA CCTGGGTCTC
    AGTGTGGAAG TATGGAACAA AGGACTGATC TGGGACACCA TGGTGGGGAC TGTGTGGATT
    GCACTGAAGA CGATTCGTCA GTCGGATGAG GAAGGGCCTG GGGAATGGTC CACGTTGGAG
    GCAGAGACAT TAATGAAAGA TGATGAAATC TGTGGAACTA AAAACCCAAC TCCCCATAAA
    ATTTTGCTTG ATACAAGATT TGAGTTGCCT TTTGATATCC CAGAAGAGGA AGCTAGATAT
    TGGACCTACA GATTGGAGCA AATGAATGCC TTGGGACCTG ACAATGAGTA TTCTAGTCAA
    GAAGAAAGCC AGAGGAAGCA ATTGCCTACT GCTGCCGCCC AGTGTTCTTT TGAAGATCCT
    GACAGTGCCG TGGATGACCG AGATAGTGAT TATCGCAGTG AGACCAGCAA TAGCATCCCG
    CCTCCTTACC ATACAACTGC CCAGCCCAAC GCTTCTGTGC ACCAGTTCCC AGTGCCTGTG
    CGATTGCCAC AGCAGCTGCT GCTTCAGGGC AGTTCCCGGG ACTCTTGCAA TGACTCTGTG
    CAAAGTTATG ACCTTGAGTA TCCAGAGCGG CGGGCCCTCA GCCCCACCAG CAGTAGTAGG
    TATGGCTCCT CCTGTAACGT GAGTCAAGGA AGCTCTCAGT TAAGTGAACT GGACCAGTGT
    CATGAACAAG ATGATGACCG TCGGGAGAGG GACTCCATTC ATTCCTGCCA CAGCTCCAGC
    AGCCTCTCCA GGGATGGCCA AGTGGGTTTT GGAGAACAAG AGAAAGCCTT GGAGGTGACA
    GGTGAAGAGG AGAAGAGGGG AGTGTGTGAA CCCAAGGAGA TGAAAGAAGA TGCCATGGCC
    CATCCACCCC CAGATATGGT GCTACACAAA GACCTTGTCC GAAGCCCCCA GGAGAGTTTT
    CCTGAGGAGA CTGCATCTTC GCCATTTACC CAAGCCAGAG CGCACTGGAT CCGAGCAGTT
    ACCAAGGTTC GACTCCAGCT GCAGGAGATT CCAGATGGTG GTGATCCCTG TATGCCCCAG
    TGGCTTCCAG AAGGGCCAGC TGGAGGGCTC TATGGCATTG ACAGCATGCC GGATCTACGC
    AGAAAGAAGC CACTGCCGCT TGTCAGCGAC CTGTCACTGG TCCAGTCCCG GAAAGCAGGC
    ATTACTTCTG CAATGGCTAC ACGCACCTCT CTCAAAGATG AAGAGCTGAA ATCACATGTG
    TATAAGAAAA CCCTGCAGGC CTTAATCTAT CCCATCTCGT GCACCACACC CCACAACTTT
    GAGGTGTGGT CAGCCACCAC CCCCACCTAC TGCTATGAGT GTGAAGGCCT GCTCTGGGGC
    ATTGCCCGGC AGGGCATGCG CTGCAGCGAG TGTGGAGTCA AGTGCCATGA GAAGTGCCAG
    GACCTACTCA ATGCTGACTG CCTGCAGCGG GCTGCAGAAA AGAGCTCTAA ACATGGAGCG
    GAGGACCGGA CCCAGAACAT CATCATGGCC ATGAAGGACC GCATGAAGAT CCGAGAGCGG
    AATAAGCCAG AGATCTTTGA AGTTATCCGG GATGTCTTCA ATGTGAGCAA AGTCGCCCAT
    GTGCAACAAA TGAAAACAGT GAAGCAGAGT GTGTTGGATG GCACCTCCAA ATGGTCAGCC
    AAAATCACCA TCACTGTGGT GTGTGCCCAG GGCCTACAAG CCAAGGACAA GACAGGATCC
    AGTGACCCTT ATGTGACTGT GCAAGTGGGC AAAACCAAGA AGCGTACCAA GACCATTTTT
    GGAAACCTGA ATCCTGTTTG GGAGGAGAAG TTCCATTTTG AGTGCCACAA CTCTTCTGAC
    CGCATTAAGG TACGTGTGTG GGATGAGGAT GATGACATCA AGTCACGAGT AAAGCAGCGG
    CTAAAGCGAG AGTCTGATGA TTTCCTCGGC CAAACCATCA TTGAGGTTCG AACACTGAGT
    GGCGAAATGG ACGTCTGGTA CAACTTGGAG AAGAGGACAG ACAAGTCAGC CGTCTCGGGG
    GCCATCCGAC TGCAAATCAG CGTGGAGATC AAGGGAGAAG AGAAGGTAGC CCCATATCAT
    GTGCAGTACA CGTGTCTCCA TGAGAACCTC TTCCATTACC TCACGGACAT TCAGGGCAGC
    GGAGGAGTCC AGATCCCTGA GGCTCGAGGA GATGATGCAT GGAAGGTGTA CTTTGATGAG
    ACTGCCCAGG AAATTGTGGA TGAATTTGCC ATGCGCTATG GCATTGAGTC CATATATCAG
    GCCATGACGC ATTTTGCATG TTTGTCATCC AAGTACATGT GTCCTGGTGT GCCAGCAGTG
    ATGAGCACCT TACTGGCCAA CATCAATGCC TACTATGCCC ACACAACTGC CTCCACCAAT
    GTCTCTGCAT CTGATCGTTT TGCAGCCTCC AACTTTGGGA AAGAGAGATT TGTGAAACTT
    CTGGACCAGC TGCACAACTC ACTGAGGATT GACCTCTCTA CCTACAGGAA TAATTTCCCT
    GCTGGGAGCC CTGAGCGACT TCAGGACTTA AAGTCCACAG TGGATTTGCT GACCAGCATT
    ACTTTCTTCC GAATGAAGGT GCAAGAACTT CAGAGCCCTC CAAGAGCCAG CCAGGTAGTA
    AAAGATTGTG TGAAGGCCTG TTTGAACTCA ACATATGAGT ATATCTTCAA CAACTGCCAT
    GACTTGTACA GCCGCCAGTA CCAGCTGAAT CAGGAGCTGC CTCCAGAAGA ACAGGGGCCC
    AGCATTAGGA ACCTGGATTT CTGGCCCAAA CTCATCACAC TCATTGTGTC AATCATAGAG
    GAAGATAAGA ATTCCTACAC CCTCGTTCTG AACCAGTTTC CCCAGGAGTT GAATGTGGGA
    AAAGTCAGCG CAGAAGTGAT GTGGCAACTC TTTGCCCAAG ACATGAAATA TGCACTGGAG
    GAGCATGAGA AAGACAGGCT GTGTAAGAGT GCTGACTACA TGAACCTGCA CTTCAAGGTG
    AAGTGGCTCC ACAATGAATA CGTGCAGGAT CTGCCTGCCC TCCAGGGCCA GGTGCCCGAA
    TACCCAGCGT GGTTTGAGCA GTTTGTCCTG CAGTGGCTGG ACGAGAATGA GGATGTGTCC
    TTGGAATTCC TGCGTGGGGC CTTGGAACGA GATAAGAAGG ATGGGTTCCA GCAGACATCA
    GAGCACGCGC TCTTCTCTTG CTCTGTGGTG GACGTCTTCA CACAGCTCAA TCAGAGCTTT
    GAGATCATCC GGAAATTGGA ATGCCCAGAC CCCAACATCC TTGCCCACTA CATGAGGAGA
    TTTTCTAAGA CCATCGGGAA GGTGCTCATG GAATATGCAG ACATCTTATC AAAGAACTTC
    CCAGCTTACT GCACAAAGGA GAAAATGCCT TGCATCCTGA TGAACAACAT GCAGCAGCTG
    AGGGTGCAGC TGGAGAAGAT GTTTGAGGCC ATGGGAGGCA AGGAGCTGGA CCCTGAAACT
    GCAGACAGTC TGAAGGAGCT TCAGGTGAAA CTGAATACAG TTTTGGATGA GCTCAGCATG
    GTGTTTGGAA ACAGTTTCCA GGTGCGGATT GATGAGTGCG TGCGACAAAT GGCCGACATC
    CTGAGCCAGG TGCGGGGCCC AGGGAACGCA TCCCCCAACG CACGGGCCTC GGTGACTCAG
    GATGCAGACA GTGTGCTCCG GCCTCTCATG GACTTCCTGG ACGGCAACCT CACACTTTTT
    GCCACTGTGT GTGAGAAGAC GGTCCTGAAG CGGGTACTGA AGGAACTCTG GCGGGTGGTA
    ATGAACACAA TGGAAAGGAT GATTGTTCTG CCTCCACTTA CTGACCAGAC GGGCACCCAG
    CTGATCTTCA CTGCTGCCAA GGAGCTGAGC CAACTTTCCA AACTCAAGGA CCACATGGTG
    CGAGAGGAAA CACGGAATCT GACTCCAAAG CAGTGTGCCG TCCTTGACCT CGCCCTGGAC
    ACCATCAAGC AATACTTTCA CGCCGGAGGC AACGGGCTGA AGAAAACCTT CCTGGAGAAG
    AGCCCAGATC TGCAGTCCCT GCGCTATGCC CTATCTCTGT ACACACAGAC CACAGACACC
    CTCATCAAGA CCTTTGTGCG CTCACAGACT GCCCAGGTGC ATGATGGGAA AGGTATTAGG
    TTTACTGCTA ATGAGGACAT TCAGCCGGAA AAGGGGTCCG GTGTGGATGA TCCTGTGGGA
    GAAGTCTCTA TTCAGGTGGA CTTGTTTACA CATCCTGGAA CTGGGGAGCA CAAGGTCACA
    GTGAAAGTGG TGGCTGCCAA TGACCTCAAG TGGCAGACAG CAGGTATGTT CCGGCCCTTT
    GTCGAAGTCA CCATGGTTGG CCCTCACCAA AGTGATAAAA AGAGGAAGTT CACAACCAAG
    TCAAAAAGTA ACAACTGGGC TCCCAAGTAC AACGAGACGT TTCACTTCCT CCTGGGAAAT
    GAAGAGGGGC CAGAAGCCTA TGAGTTGCAG ATATGCGTGA AGGATTACTG CTTTGCCCGG
    GAGGACCGTG TGCTGGGGCT GGCTGTAATG CCTCTGAGGG ATGTGGCAGC CAAGGGCAGC
    TGTGCTTGCT GGTGCCCCTT GGGCCGTAAG ATCCACATGG ATGAGACAGG CGTGACCATT
    CTCCGGATTC TGTCTCAGAG GAGCAATGAC GAAGTGGCCC GGGAGTTTGT GAAGCTCAAA
    TCAGAGTCTC GTTCTATGGA GGAGGGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.