COL6A3 cDNA ORF clone, Bos taurus(cattle)

The following COL6A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa03695 XM_005197963.3
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03695 XM_005204978.3
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03697 XM_005197964.3
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03697 XM_005204979.3
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03698 XM_002686571.5
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03698 XM_003582038.4
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03699 XM_005204980.3
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03699 XM_005197965.3
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa03699 XM_024990265.1
Latest version!
Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa233420 XM_024990262.1
Latest version!
Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa233421 XM_024990263.1
Latest version!
Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa233422 XM_024990264.1
Latest version!
Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa51858 XM_010827157.2
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa51858 XM_024990266.1
Latest version!
Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa51858 XM_010803869.2
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa51859 XM_010803870.2
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa51859 XM_010827158.2
Latest version!
Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa51859 XM_024990267.1
Latest version!
Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa03695
    Clone ID Related Accession (Same CDS sequence) XM_005197963.3 , XM_005204978.3
    Accession Version XM_005197963.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC
    TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT
    GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG
    CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG
    TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG
    GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC
    CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA
    GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC
    GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT
    TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC
    GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005198020.1
    CDS348..10043
    Translation

    Target ORF information:

    RefSeq Version XM_005197963.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005197963.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC
    TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT
    GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG
    CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG
    TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG
    GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC
    CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA
    GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC
    GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT
    TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC
    GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03695
    Clone ID Related Accession (Same CDS sequence) XM_005197963.3 , XM_005204978.3
    Accession Version XM_005204978.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204978.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC
    TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT
    GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG
    CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG
    TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG
    GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC
    CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA
    GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC
    GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT
    TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC
    GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205035.1
    CDS348..10043
    Translation

    Target ORF information:

    RefSeq Version XM_005204978.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204978.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC
    TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT
    GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG
    CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG
    TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG
    GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC
    CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA
    GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC
    GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT
    TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC
    GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03697
    Clone ID Related Accession (Same CDS sequence) XM_005197964.3 , XM_005204979.3
    Accession Version XM_005197964.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
    1681
    1741
    1801
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    1981
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    2641
    2701
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    8401
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    8641
    8701
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    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005198021.1
    CDS346..9441
    Translation

    Target ORF information:

    RefSeq Version XM_005197964.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005197964.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03697
    Clone ID Related Accession (Same CDS sequence) XM_005197964.3 , XM_005204979.3
    Accession Version XM_005204979.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205036.1
    CDS346..9441
    Translation

    Target ORF information:

    RefSeq Version XM_005204979.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204979.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCATAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03698
    Clone ID Related Accession (Same CDS sequence) XM_003582038.4 , XM_002686571.5
    Accession Version XM_003582038.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582038.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT
    GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT
    CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC
    GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT
    GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT
    TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC
    CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA
    GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA
    GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC
    CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT
    GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT
    TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC
    ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT
    CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG
    GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC
    GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG
    GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG
    CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT
    GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG
    TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC
    ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG
    TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC
    AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG
    GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG
    CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT
    GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA
    CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG
    CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG
    GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT
    TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG
    CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC
    CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT
    GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG
    TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG
    CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC
    TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG
    GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG
    GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA
    CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA
    CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA
    AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG
    AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG
    GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC
    CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC
    ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC
    ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC
    GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT
    GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC
    ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG
    CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG
    GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG
    TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC
    ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC
    GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG
    AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC
    CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG
    TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC
    ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC
    GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG
    CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT
    CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG
    AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC
    GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA
    TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG
    TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC
    TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT
    CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA
    ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC
    AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA
    AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA
    CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC
    ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC
    ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC
    ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC
    AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG
    CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC
    TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG
    CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG
    AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA
    CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC
    GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA
    GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG
    AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG
    TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG
    CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG
    GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG
    GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG
    GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC
    TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG
    CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA
    GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC
    AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA
    GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC
    CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA
    GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT
    GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG
    AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA
    GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG
    TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA
    GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA
    GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT
    CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG
    CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG
    GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA
    GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG
    CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA
    CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA
    GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC
    CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG
    GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG
    ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG
    GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC
    GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG
    GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG
    ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG
    GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG
    GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC
    CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC
    TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG
    GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC
    GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA
    CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG
    CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC
    TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC
    CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC
    GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG
    CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC
    TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC
    AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG
    CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC
    CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT
    CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA
    TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG
    AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG
    AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC
    AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC
    AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA
    AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA
    AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT
    GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG
    GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC
    GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG
    CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT
    CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT
    TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC
    TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA
    ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG
    TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA
    ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT
    TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC
    GCCGCGATGG GGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582086.2
    CDS346..9423
    Translation

    Target ORF information:

    RefSeq Version XM_003582038.4
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582038.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT
    GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT
    CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC
    GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT
    GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT
    TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC
    CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA
    GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA
    GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC
    CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT
    GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT
    TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC
    ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT
    CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG
    GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC
    GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG
    GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG
    CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT
    GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG
    TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC
    ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG
    TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC
    AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG
    GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG
    CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT
    GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA
    CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG
    CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG
    GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT
    TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG
    CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC
    CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT
    GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG
    TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG
    CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC
    TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG
    GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG
    GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA
    CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA
    CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA
    AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG
    AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG
    GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC
    CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC
    ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC
    ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC
    GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT
    GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC
    ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG
    CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG
    GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG
    TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC
    ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC
    GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG
    AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC
    CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG
    TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC
    ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC
    GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG
    CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT
    CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG
    AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC
    GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA
    TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG
    TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC
    TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT
    CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA
    ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC
    AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA
    AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA
    CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC
    ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC
    ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC
    ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC
    AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG
    CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC
    TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG
    CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG
    AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA
    CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC
    GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA
    GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG
    AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG
    TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG
    CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG
    GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG
    GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG
    GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC
    TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG
    CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA
    GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC
    AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA
    GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC
    CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA
    GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT
    GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG
    AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA
    GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG
    TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA
    GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA
    GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT
    CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG
    CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG
    GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA
    GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG
    CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA
    CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA
    GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC
    CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG
    GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG
    ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG
    GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC
    GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG
    GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG
    ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG
    GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG
    GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC
    CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC
    TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG
    GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC
    GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA
    CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG
    CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC
    TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC
    CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC
    GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG
    CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC
    TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC
    AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG
    CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC
    CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT
    CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA
    TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG
    AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG
    AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC
    AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC
    AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA
    AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA
    AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT
    GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG
    GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC
    GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG
    CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT
    CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT
    TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC
    TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA
    ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG
    TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA
    ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT
    TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC
    GCCGCGATGG GGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03698
    Clone ID Related Accession (Same CDS sequence) XM_003582038.4 , XM_002686571.5
    Accession Version XM_002686571.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002686571.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT
    GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT
    CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC
    GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT
    GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT
    TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC
    CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA
    GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA
    GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC
    CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT
    GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT
    TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC
    ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT
    CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG
    GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC
    GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG
    GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG
    CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT
    GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG
    TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC
    ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG
    TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC
    AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG
    GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG
    CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT
    GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA
    CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG
    CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG
    GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT
    TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG
    CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC
    CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT
    GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG
    TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG
    CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC
    TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG
    GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG
    GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA
    CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA
    CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA
    AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG
    AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG
    GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC
    CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC
    ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC
    ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC
    GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT
    GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC
    ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG
    CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG
    GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG
    TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC
    ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC
    GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG
    AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC
    CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG
    TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC
    ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC
    GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG
    CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT
    CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG
    AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC
    GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA
    TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG
    TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC
    TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT
    CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA
    ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC
    AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA
    AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA
    CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC
    ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC
    ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC
    ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC
    AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG
    CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC
    TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG
    CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG
    AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA
    CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC
    GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA
    GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG
    AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG
    TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG
    CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG
    GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG
    GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG
    GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC
    TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG
    CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA
    GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC
    AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA
    GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC
    CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA
    GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT
    GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG
    AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA
    GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG
    TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA
    GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA
    GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT
    CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG
    CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG
    GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA
    GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG
    CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA
    CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA
    GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC
    CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG
    GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG
    ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG
    GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC
    GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG
    GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG
    ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG
    GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG
    GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC
    CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC
    TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG
    GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC
    GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA
    CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG
    CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC
    TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC
    CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC
    GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG
    CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC
    TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC
    AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG
    CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC
    CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT
    CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA
    TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG
    AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG
    AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC
    AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC
    AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA
    AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA
    AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT
    GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG
    GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC
    GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG
    CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT
    CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT
    TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC
    TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA
    ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG
    TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA
    ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT
    TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC
    GCCGCGATGG GGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002686617.2
    CDS346..9423
    Translation

    Target ORF information:

    RefSeq Version XM_002686571.5
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002686571.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT
    GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT
    CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC
    GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT
    GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT
    TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC
    CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCATA
    GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA
    GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC
    CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT
    GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT
    TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC
    ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT
    CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG
    GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC
    GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG
    GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG
    CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT
    GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG
    TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC
    ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG
    TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC
    AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG
    GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG
    CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT
    GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA
    CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG
    CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG
    GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT
    TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG
    CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC
    CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT
    GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG
    TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG
    CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC
    TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG
    GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG
    GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA
    CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA
    CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA
    AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG
    AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG
    GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC
    CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC
    ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC
    ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC
    GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT
    GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC
    ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG
    CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG
    GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG
    TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC
    ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC
    GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG
    AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC
    CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG
    TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC
    ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC
    GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG
    CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT
    CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG
    AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC
    GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA
    TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG
    TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC
    TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT
    CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA
    ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC
    AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA
    AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA
    CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC
    ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC
    ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC
    ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC
    AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG
    CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC
    TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG
    CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG
    AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA
    CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC
    GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA
    GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG
    AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG
    TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG
    CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG
    GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG
    GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG
    GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC
    TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG
    CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA
    GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC
    AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA
    GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC
    CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA
    GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT
    GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG
    AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA
    GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG
    TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA
    GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA
    GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT
    CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG
    CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG
    GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA
    GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG
    CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA
    CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA
    GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC
    CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG
    GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG
    ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG
    GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC
    GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG
    GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG
    ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG
    GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG
    GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC
    CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC
    TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG
    GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC
    GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA
    CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG
    CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC
    TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC
    CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC
    GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG
    CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC
    TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC
    AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG
    CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC
    CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT
    CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA
    TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG
    AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG
    AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC
    AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC
    AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA
    AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA
    AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT
    GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG
    GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC
    GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG
    CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT
    CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT
    TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC
    TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA
    ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG
    TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA
    ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT
    TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC
    GCCGCGATGG GGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03699
    Clone ID Related Accession (Same CDS sequence) XM_005197965.3 , XM_024990265.1 , XM_005204980.3
    Accession Version XM_005197965.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC
    AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC
    CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG
    ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG
    ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC
    AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC
    CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC
    GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG
    ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG
    CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT
    CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005198022.1
    CDS343..8817
    Translation

    Target ORF information:

    RefSeq Version XM_005197965.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005197965.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC
    AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC
    CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG
    ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG
    ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC
    AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC
    CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC
    GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG
    ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG
    CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT
    CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03699
    Clone ID Related Accession (Same CDS sequence) XM_005197965.3 , XM_024990265.1 , XM_005204980.3
    Accession Version XM_024990265.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3601
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    3721
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    4021
    4081
    4141
    4201
    4261
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    4381
    4441
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    4621
    4681
    4741
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    4921
    4981
    5041
    5101
    5161
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    5281
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    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC
    AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC
    CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG
    ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG
    ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC
    AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC
    CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC
    GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG
    ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG
    CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT
    CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846033.1
    CDS276..8750
    Translation

    Target ORF information:

    RefSeq Version XM_024990265.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990265.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC
    AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC
    CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG
    ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG
    ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC
    AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC
    CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC
    GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG
    ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG
    CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT
    CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03699
    Clone ID Related Accession (Same CDS sequence) XM_005197965.3 , XM_024990265.1 , XM_005204980.3
    Accession Version XM_005204980.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC
    AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC
    CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG
    ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG
    ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC
    AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC
    CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC
    GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG
    ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG
    CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT
    CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205037.1
    CDS343..8817
    Translation

    Target ORF information:

    RefSeq Version XM_005204980.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204980.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGTTCGCGT AAACAAAAGG GATATCATCT TTCTTTTGGA TGGATCGTCC
    AACGTCGGAG AGACCAATTT CCCTTATGTG CGGGACTTTG TCATGAACCT AGTTAACAGC
    CTTGATGTTG GAAGCGACCA CATTCGGGTC GGTCTAGTGC AATTTAGTGA CACTCCGGTG
    ACGGAGTTCT CCCTAAACAC CTACCCGACC AAGTCAGAGT TGCTAGCTCA CCTGAGGCAG
    ATGCAGCTCC AGGGGGGGTC GGTCCTGAAC ACGGGCGCGG CCCTGAGCTA TGTGCATGCC
    AACCACTTCA CCGAAGCTGG CGGCAGCAGG ATCCAGGACC ACGTGCCGCA GCTGCTACTC
    CTGCTCACGG CCGGGCAGTC TGAGGACTCC TATTTGCAAG CCGCCAACGC CCTGGCGCGC
    GCGGGCATCC TGACCTTTTG TGTGGGCACT AGCCAGGCCG ATAGGGCGGA GCTTGAGGAG
    ATTGCTTTTA ACCCGGGCCT GGTGTATCTC ATGGATGATT TCAGCTCCTT GCCAGCTTTG
    CCTCAACAGC TGATTCAGCC CCTAACCACT TATGTTAGCG GAGGTGTGGA GGAGGTGCCT
    CTTGCCCAGC CAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233420
    Clone ID Related Accession (Same CDS sequence) XM_024990262.1
    Accession Version XM_024990262.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC
    TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT
    GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG
    CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG
    TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG
    GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC
    CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA
    GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC
    GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT
    TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC
    GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846030.1
    CDS278..9973
    Translation

    Target ORF information:

    RefSeq Version XM_024990262.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990262.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGTTCGCG TAAACAAAAG GGATATCATC
    TTTCTTTTGG ATGGATCGTC CAACGTCGGA GAGACCAATT TCCCTTATGT GCGGGACTTT
    GTCATGAACC TAGTTAACAG CCTTGATGTT GGAAGCGACC ACATTCGGGT CGGTCTAGTG
    CAATTTAGTG ACACTCCGGT GACGGAGTTC TCCCTAAACA CCTACCCGAC CAAGTCAGAG
    TTGCTAGCTC ACCTGAGGCA GATGCAGCTC CAGGGGGGGT CGGTCCTGAA CACGGGCGCG
    GCCCTGAGCT ATGTGCATGC CAACCACTTC ACCGAAGCTG GCGGCAGCAG GATCCAGGAC
    CACGTGCCGC AGCTGCTACT CCTGCTCACG GCCGGGCAGT CTGAGGACTC CTATTTGCAA
    GCCGCCAACG CCCTGGCGCG CGCGGGCATC CTGACCTTTT GTGTGGGCAC TAGCCAGGCC
    GATAGGGCGG AGCTTGAGGA GATTGCTTTT AACCCGGGCC TGGTGTATCT CATGGATGAT
    TTCAGCTCCT TGCCAGCTTT GCCTCAACAG CTGATTCAGC CCCTAACCAC TTATGTTAGC
    GGAGGTGTGG AGGAGGTGCC TCTTGCCCAG CCAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233421
    Clone ID Related Accession (Same CDS sequence) XM_024990263.1
    Accession Version XM_024990263.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846031.1
    CDS277..9372
    Translation

    Target ORF information:

    RefSeq Version XM_024990263.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAT GTCAAAAATG GTGCCGCTGC CGATATAATA
    TTTCTAGTGG ATTCCTCTTG GAGCATTGGC AAGGAACATT TCCAACTTGT TCGAGAGTTT
    CTATATGATG TTATAGAATC TTTAGCAGTG GGAGACAATG ATTTCCGTTT TGCTCTGGTC
    CAGTTCAACG GCAACCCACA TACCGAGTTC CTGTTCAATA CGTATCGTTC TAAACAAGAA
    GTCCTCTCCC ATGTTTCCAA CATGTCTTAT ATTGGGGGCA GCAATCAGAC TGGAAAAGGA
    TTAGCATACG TAATGCAGAA CCACCTCACT GAGGCTGCCG GAAGCCGGGC CAGTGACGGA
    GTCCCTCAGG TTATCGTAGT GTTAACCCAT GGACACTCGG AGGACGGCCT TGCTCTGCCC
    TCAGCGGAAC TTAAGTCTGC TGATGTTAAC GTGTTTGCAA TTGGAGTTGA GGATGCAGAT
    GAAGCAGCCT TAAAAGAAAT AGCAAGTGAA CCGCTCAATA TGCATGTTTT CAACCTAGAG
    AATTATACCT CACTTCATGA CATAGTAGGA AACTTAGTGG CCTGTGTGCG TTCATCCATG
    GCTCCAGAAA GGGCTGGAGG CACAGAGATC CCCAAAGACA TCACAGCACA AGACTCTGCT
    GACATTATTT TCCTTATTGA TGGATCAAAC AACACCGGAA GTGTCAATTT CGCAGTCATT
    CTCGACTTCC TTGTAAATCT CCTTGAGAGA CTGTCAATTG GAACTCAGCA GATCCGAGTG
    GGGGTGGTGC AGTATAGCGA TGAGCCCAGA ACCATGTTCT CCTTGAACAG CTACTCCACC
    AAGGCTCAGG TTCTGGATGC AGTGAAGGCC CTGGGGTTCA TTGGTGGAGA GCTGGCCAAT
    GTTGGCCTTG CCCTGGATTT TGTGGTGGAG AATCACTTCA CCCGGGCGGG GGGCAGCCGA
    GCAGAGGAAG GGGTTCCCCA GGTGCTGGTC CTCATAAGTG CTGGGCCTTC TAGTGATGAG
    ATCCGAGACG GGGTCGTAGC ACTGAAGCAG GCTAGCGTTT TCTCGTTTGG CCTCGGAGCC
    CAGGCCGCCT CCAAGGCAGA GCTTCAGCAC ATAGCTACCA ATGACAACCT GGTGTTTACT
    GTCCCGGAAT TCCGTAGCCT TGGGGACGTC CAGGAACAAT TACTGCCGTA CATTGTTGGC
    GTGGCCCAAA GGCACATTGT CTTGCAACCG CCAACCATTG TCACCCAAGT CATTGAAGTC
    AACAAGAGGG ACATAGTTTT CCTGGTGGAT GGCTCTTCCA AACTGGGACT GACCAACTTC
    AATGCAATCC GCGACTTCAT TGCCAAGGTC ATCCAGAGGC TGGAGATCAG ACAGGATCTT
    ATCCAGGTGG CAGTGGCTCA GTATGCAGAC ACAGTGAGGC CAGAGTTTTA TTTCAATACC
    TACCCCAGCA AGAGGGAGGT CATAAACGCC GTGCGGAAAA TGAAGGCTTT GGATGGCTCA
    GCCTTGTACA CGGGCTCCGC TCTGGATTTT GTTCGGAACA ACCTGTTCAC GGAGGCGGCC
    GGCTACCGGG CTGCCGAGGG GGTCCCTAAG CTTCTGGTGC TGGTTACAGG CGGTAAGTCC
    CTAGATGCGG TCAGCCAGCC CGCCCAGGAG CTGAAGAGGA GCGGCATCCT GGCCTTCGCC
    GTCGGGAACA AGGTGGCTGA CCAGGCTGAA CTGGAAGAGA TCGCTTTCGA TTCCTCCCTG
    GTGTTCACTG CCACCGAGTT CCGACCCGCC CCTTTGCAGG GCGTGCTGCC CGGCTTGCTC
    GGGCCTCTCA GGACCCTCAC TGGAACCACT GAAGAGAGCA AGCGAGACAT CCTGTTCCTT
    TTCGACGGCT CAGCCAACCT CATGGGCCAG TTCACTGCCG CCCGTGACTT TCTCTACAAG
    GTCATCGACG AGCTGGATGT GAAACCTGAG GGGACCCGGG TTGCAGTGGC TCAGTTCAGT
    GATGATGTCA AGGTGGAGTC CCGTTTTGAC CAGCACCAGA ATAAGCCTGA GATCCTGAAT
    CTCGTGAAGA GGATGAAGCT CAAGACAGGC AAAGCCCTCA ACCTGGGCTA CGCCTTGGAT
    TACGCACAGA GGTACATCTT TGTGAAGTCC GCTGGAAGCC GGATTGAGGA TGGAGTGCTT
    CAGTTCCTGG TGCTGCTGGT GGCGGGAAAG TCATCGGACC GGGTGGATAC ACCCGCACTC
    AACCTGAAGC AGAGCGGGGT GGTGCCCTTC ATCCTCCAGG CGAAAAACGC GGACCCTGCT
    GAGTTGGAGC TGATCGTACC ATCCCCAGCC TTTATTTTGG TTGCGGAGTC CCTTCCTAAG
    ATTGGAGACC TGCAACCACA GATTGTGAAC CTCTTAAAAT CAGTGCAAAA TGGGGCACCG
    GCACCAGTTT CAGTTGAAAA GGATGTGGTG TTCCTGATTG ATGGCTCCGA GGGTGTCAGG
    AGCGGCTTCC CACTGCTGAA GGAGTTCGTC CAGAGAGTGG TGGAGAGCCT GGACGTGGGA
    CCAGACCGGG TGCGTGTGGC CGTGGTGCAG TACAGCGACC GGACCAGACC TGAGTTCTAC
    CTGAATTCCT ACATGGACCA GCAGAGTGTG GTCGGAGCCA TCCGTGGGCT GACCCTGCTG
    GGCGGGCCGG CCCCCAACAC AGGGGCTGCC CTGGAGTTCG TCCTGAGGAA CATCCTGGTG
    GGCTCTGCTG GGAGCCGCAT AGCAGAAGGT GTCCCCCAGC TCCTGATTGT CCTCACGGCC
    GACAGGTCTG GGGATGACGT GAGGGGCCCC TCTGTGGTCC TGAGGAGGGG AGGGGCCGTG
    CCCATCGGCA TCGGCATTGG GAACGCTGAC ATCACCGAGA TGCAGACCCT CTCCTTCGTC
    CCGGACTTCG CCGTGGTCAT CCCCACCTTC CGGCAGCTGG GGACCATCCA ACAGGTCATC
    TCGGAGAGAG TGACCCAGCT CAGCCGCGAG GAACTGAGCA GGCTGCAGGC GTCTGTCACC
    CCCTTGACGA CTCCGGTGGT CAGCAGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC
    CAGAGTGCTG GCCCCGAGTT CCAGTACATC CGAACGCTCA TCGAGAGGCT GGTGGACTAC
    CTGGATGTGG GCTTCGACAC GACCCGGGTG GCGGTCATCC AGTTCAGCGA TGACCCCAGG
    GTGGAGTTCC TGTTGAACGT CCACTCCAGC AAGGACGAGG TGCAGAACGC CGTGCGGCGG
    CTGCGGCCCA AAGGTGGGAG GCAGGTCAAC GTTGGGGGTG CCCTGGAGTA CGTGGCCAGG
    AACATCTTCA AGAGGCCCCT GGGGAGCCGG ATTGAGGAGG GTGTCCCTCA GTTCCTGGTC
    CTCATTTCCT CCGGGAAGTC GGATGATGAG GTGGAGGACT CGGCCATAGA GCTCAAGCAG
    TTTGGCGTGG CGCCACTCAC GATCGCCAGG AACGTGGACC AGGAAGAGCT GGTGAAGATC
    TCGCTGAGCC CCGAGTACGT GTTCTCAGTG AACACCTTCC GGGAGCTCCC CAGCCTGGAG
    CAGAAACTGC TGACACCGCT CACCACCCTG ACTGCCGGGC AGATACAGCA GCTCCTGGCC
    AGCACGCGCT ACCCGCCTCC AGCTGTGGAG AGCGATGCTG CGGACATTGT CTTCCTCATC
    GACAGCTCTG ATGGCGTGAA GCCGGATGGC ATCGCACACA TCCGGGACTT TGTTATCAGG
    ATCGTGCGGA GACTCAACGT CGGTCCCAAC AAAGTGAGAA TTGGGGTCCT GCAGTTCAGC
    AACGACGTCT TCCCAGAATT TCAACTAAAG ACCTATAAAT CCCAGGCCAG CGTGCTGGAT
    GCCATACGTC GCCTGAGGTT CAAAGGGGGG TCTCCTCTCA ACACTGGCAA GGCTCTGGAA
    TTCGTGGCGA GGAATTACTT TGTCAAGTCT GCCGGGAGCC GGATAGAAGA TGGGGTGCCC
    CAACACCTGG TTCTATTTCT GGGTGGAAAA TCCCAGGATG ATATTTCCAG GTATTCCCAG
    GTGATAAAAT CTGCAGGAAT TGCGAGCTTA GGGGTTGGAG ACCGAAATAT TGACAGAACA
    GAGCTGCAGA CCATCACCAG CGATCCCAGG CTTGTCTTCA CGGTGCGGGA GTTCAGAGAC
    CTCCCCAGCA TAGAAGAAAG GATGGTGAAT TCCTTTGGGT CCTCCGGGGT CACCCCTGCA
    CCTCCGGGAG TGGACACACC CTCTCCTTCA AGGCCAGAGA AGAAGAAAGC CGACATTGTG
    TTCCTGCTGG ATGGTTCCAT CAATTTCCGG AGAGACAGTT TCCAGGAAGT GCTCCGTTTT
    GTGTCAGAAA TTGTGGACAC GGTTTATGAA GGGGGCGACT CCATCCAGGT GGGGCTGGTC
    CAATACAACT CTGACCCCAC CGACGAGTTC TTCCTGAAGG ACTTCCCCAC CAAGCAGCAG
    ATTATCGACG CCATAAACAA GGTGGTCTAC AAAGGGGGGA GGCACGCGAA CACCAAGGTG
    GGCCTGGAGC ACCTGCGGCG GAACCACTTC GTGCCCGAGG CGGGCAGCCG CCTGGACCAG
    AGGGTCCCGC AGATCGCCTT TGTGATCACG GGGGGCAAGT CGGTGGAGGA CGCTCAGGAG
    GCAAGCATGG CGCTCACGCA GAGAGGCGTC AAGGTGTTTG CGGTGGGCGT AAGGAACATC
    GACTCGGAGG AGGTTGGGAA GATAGCGTCC AACAGCGCCA CGGCCTTCCG GGTGGGGAAC
    GTCCAGGAGC TGTCGGAACT GAGCGAGCAG GTTTTGGAAA CTCTGCACGA TGCAATGCAT
    GAGACCTTAT GTCCAGGCGT GACTGACGTT TCCAAAGCCT GTAACTTGGA TGTGATTCTG
    GGCTTCGATG GTTCAGGAGA TCAGAATGTC TTTGTGGCTC AGAAGGGCCT CGAGCCCAAG
    GTGGACACCA TCCTGAGGAG AATCAGTCAG ATGCAGAAGA TCAGCTGCAG CGGCAGCCAG
    CTGCCCACCG TGCGGGTGTC TGTGGTGGCC CTCACGCCCT CGGGCCCCGT TGAGGCCTTT
    GACTTTGCTG AGTACCAGCC GGAGCTGTTT GAGAAGTTCC AGAACATGCG TGCCCAGCAC
    CCCTACGTCC TCACTGCGGA CACACTGAAG CTCTACCAGA ACAAGTTCCG GCAGGCCTCG
    ATGGACAACG TGAAGGTGGT CATTCATTTT ACCGATGGAG TGGATGGGGA TCTGGCTGAT
    TTACAAAGAG CATCAGAGGA ACTCCGACAA GAAGGTGTCC GAGCTCTGAT CTTGGTGGGC
    CTCGAACGCG TCGCCAACTT GGAGCAGCTC ATGCAGCTGG AGTTCGGGCG AGGGTTCACG
    TACAACCGGC CCCTGAGGCT GAACTTGCTG GACCTGGACT ACGAACTAGC CGAGCAGCTT
    GACAACATTG CTGAGAAAGC CTGCTGTGGG ATTCCCTGCA AATGCTCTGG ACAAAGAGGA
    GACCGAGGGC CCATAGGCAG CATCGGGCCA AAGGGCATTC CTGGAGAAGA TGGCTACCGA
    GGTTATCCTG GTGACGAAGG TGGACCTGGT GAACGCGGTC CTCCTGGCGT GAACGGCACT
    CAAGGTTTCC AGGGCTGCCC CGGGCAGAGA GGAGTCAAGG GCTCTCGTGG ATTCCCGGGA
    GAGAAGGGTG AATTAGGAGA AATTGGACTG GATGGTCTTG ATGGTGAAGA TGGCGACAAA
    GGGCTGCCCG GTACTTCTGG AGAGAAAGGG AGTCCAGGAA GGAGGGGCGA CAAAGGACCC
    AAAGGCGACA AAGGGGAGAG AGGCGATGTC GGGATCAGAG GCGACCCGGG CAATTCAGGA
    CAGGACAGCC AGCAGAGAGG AGCCAAAGGA GAAACCGGAG ACATCGGCCC CGTGGGTCTC
    CCTGGGAGAG ACGGGGTGTC TGGAGGGCCT GGAGAACCCG GGAAGAGCGG TGGCTTTGGC
    CGAAGGGGAC CAGCGGGAGC TAAGGGCAAC CAGGGCGGCC CCGGCCAGCA GGGCCCTGTA
    GGAGAGCAGG GGACCAGAGG CGCACAGGGT CCGCCTGGTC CCACCGGTCC TCCAGGCCTG
    ATTGGTGAAC AGGGCATTCC CGGACCTCGG GGAGGCGGGG GAACCGCAGG TGCTCCTGGA
    GAACGCGGCA GAACCGGGCC CCTGGGAAGA AAGGGTGAGC CCGGAGATCC AGGACCTAAG
    GGGAGCATCG GGAACCGGGG TCCTCGTGGG GAGACGGGAG ATGATGGGCG AGATGGAGTT
    GGCAGCGAAG GTCGCATAGG CAAGAAGGGA GAAAGAGGAT TCCCTGGATA CCCAGGTCCA
    AAGGGCAATC CTGGTGAGCC AGGGACAGAC GGACCACCGG GACCCAAAGG CATCAGAGGC
    CGAAGGGGAA ACTCGGGACC TCCAGGGATA GCTGGACAGA AGGGAGACCC CGGTTACCCA
    GGGCCATCTG GTTACAAAGG CAGCAGAGGC GACTCAATGG ACCAATGTGC GCTGGTCCAG
    AGCATCAAAG ATAAATGCCC TTGCTGTTAC GGGCCCCTGG AGTGCCCCGT CTTCCCGACG
    GAGCTGGCTT TCGCCTTGGA CACCTCAGAG GGGGTCACCC AGGACAGGTT CAGCCAGATG
    AGGGAGGTGG TCCTCAAGAT TCTGGACGAC CTGACCATCG CCGAGAGCAA CTGCCCGAGG
    GGGGCCCGCG TGGCCGTGGT CACTTACAAC AACGAGGTTA CCACGGAGAT CCGCTTTGCC
    GACTCCAAGA AGAAGTCCGT CCTCCTGGAC AAGATCAAGA ACCTGCAGGT GTCGCTGACC
    TCCAAGCAGC AGAGCCTGGA AACCGCCATG TCCTTTGTGG CCAGAAACAC GTTTAAGCGT
    GTGCGGAGCG GGTTCCTGAT GAGGAAGGTG GCGGTGTTCT TCAGCAACAA GCCCACGAGG
    GCGACCCCGC AGCTCCGGGA GGCTGTGCTC AAGCTCTCAG ATGCTGGGAT CACACCCTTG
    TTCCTCACAA GCCAGGAGGA CCGGCAGCTG ATCAATGCCC TACAGATCAA TAACACAGCG
    GTGGGACATG CCCTTGTCCT TCCTGCTGGC GGAGACCTCA CAGACTTCTT GAAGAATGTC
    CTGACGTGTC ACGTCTGCTT GGACATCTGC AACATTGACC CATCCTGTGG ATTCGGCAGC
    TGGAGGCCTT CCTTCAGGGA CCGGAGGGCA GCAGGCAGTG ATGTGGACAT CGACATGGCT
    TTCATCTTAG ATAGCTCCGA GTCCACCACC CCGTTCCAGT TCAACGAGAT GAGGAAGTAC
    ATCGAGTACC TGGTCCGACA GCTGGATGTG AGCCCAGACC CCAAGGCCTC CCAGCACTTT
    GCCCGCGTGG CGGTGGTGCA GCATGCGCCC TACGAGGCGG TGGACAACGC CAGTGTGCCG
    CCTGTGAAGG TGGAGTTCTC CCTGACCGAC TACGGCTCCA AAGAGAAGCT GCTGGCCTTC
    CTTGGCAGCA GGATGACCCA GCTGCAGGGG ACCAGGGCTT TGGGCCGCGC CATCGACTAC
    ACCATTGAGA ACATCTTCGA AAGTGCTCCC AACCCCCGGG ACCTGAAGAT CGTGGTGCTG
    ATGCTGACGG GCGAGGTGCA GAAGCAGCAG CTGGAGGAGG CCCAGAGAGC CATCCTACAG
    GCCAAGTGCA AAGGCTACTT CTTCGTGATC CTGGGCATTG GCAGGAAGGT GAATGTCAAG
    GAGCTGTACA GCTCTGCCAG CGAGCCCAAC GACGTCTTCT TCAAGCTAGT GGACAAGTCG
    ACCGAGCTCA ACGAGGAGCC CCTAATGCGC TTCGGGAGGC TGCTGCCATC CTTCGTCAGC
    AGTAAAGATG CTTTCTACCT GTCCCCAGAT ATCAGGAAAC AGTGTGATTG GTTCCAAGCA
    GACCAACCGG CAAAGAATCT CGTGCAGTTT GGTTACAAAC AAATAAATGT TCCTAATAAC
    GTTACTTCAA GTCCTACATC TAAACTGGTG ACCACAACGA AGCCGGTGAT CACCACCACC
    AAACCGATCA CAGTGGTGAA CCTGCCAGCC TCAAAGCCAG CTGCCACGAG ACCTGTGGAT
    GAGAGACCCG CAGCTGGGAG ATCCGTGGCC ACCAAGATGG ACAGTGCCAG GCCCGTGGCC
    ACCAAGACCG AGGCCACCAA ACCCGTGGCC ACCAAGACCG AGGCCACCAA GACTGAAGCT
    GCCAAACCTG TGGTCACCAA GGCTGAGGCT GCAAAACCCA CGGCCACCAA GACTGAAGCC
    ACTAAGACCG AGGCTGCAAA GCTCATGGCC ACCAAGACCG AGGCCACCAA ACCTGTGGCT
    GCCAGGCCCG AGGCGGCAAA GACAGCCACA GCCAGACCTG CAGCAGCCGC GAAGCCTGCA
    GCAGCCAAAC CTGCCCCTGC CAGACCCCCC ACTGCTGCCA GATCCATGGC CACGAGGTCC
    GAGGCCCCCA GGCCCCAGGC AGTCAAACTG GCTGCCAGCA GGCCCGGCGC TGCTAAGCCC
    GTGGTGAAGG CACCTCGCGA GATCCATGTG TCTGAGGTCA CAGAGAACAG CGCCAAGCTG
    CACTGGGAGA GGCCCGAGCC CCCCAGCCCT TACTTATACA ACCTCACCGT CACCTCGGCC
    CACGATCAGG CCGTGGTTCT GAAGCAGAAC CTCACTGTCA CGGACCGCGT CATCGGGGGC
    CTGCTCCCCG GGCAGACTTA CCACGTTACG GTGACCTGCT CCCTGAGGTC TCAGGTCAGA
    GCCATCTACC AAGGCAGCTT CAGCACGAAG AAAATTCAGC CTCCACCTCC ACAAACAGAG
    CGATCAGCCT CTAGTGCAAC CATCAACCTA GTGGTGAGCG CAGACCGGTT GGCTGGGAGT
    AAAGCAGATA TATGTAAGTT GCCAAAGGAC GGCGGAACTT GCAGAGAGTT TGTATTAAAG
    TGGTACTATG ATTCCGTAAC TGAAAACTGC GCCCGATTCT GGTATGGAGG CTGCGGTGGA
    AACGAAAACA GATTTAATTC GCAGGATGAA TGTGAAAAGG TTTGCCCTCC TGTGCCCATC
    AAGCAGCCCG GAGTTATCGC CGCGATGGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa233422
    Clone ID Related Accession (Same CDS sequence) XM_024990264.1
    Accession Version XM_024990264.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT
    GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT
    CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC
    GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT
    GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT
    TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC
    CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCGTA
    GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA
    GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC
    CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT
    GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT
    TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC
    ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT
    CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG
    GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC
    GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG
    GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG
    CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT
    GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG
    TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC
    ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG
    TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC
    AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG
    GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG
    CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT
    GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA
    CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG
    CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG
    GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT
    TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG
    CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC
    CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT
    GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG
    TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG
    CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC
    TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG
    GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG
    GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA
    CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA
    CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA
    AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG
    AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG
    GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC
    CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC
    ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC
    ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC
    GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT
    GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC
    ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG
    CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG
    GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG
    TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC
    ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC
    GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG
    AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC
    CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG
    TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC
    ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC
    GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG
    CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT
    CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG
    AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC
    GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA
    TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG
    TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC
    TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT
    CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA
    ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC
    AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA
    AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA
    CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC
    ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC
    ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC
    ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC
    AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG
    CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC
    TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG
    CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG
    AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA
    CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC
    GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA
    GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG
    AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG
    TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG
    CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG
    GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG
    GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG
    GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC
    TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG
    CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA
    GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC
    AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA
    GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC
    CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA
    GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT
    GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG
    AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA
    GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG
    TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA
    GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA
    GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT
    CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG
    CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG
    GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA
    GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG
    CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA
    CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA
    GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC
    CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG
    GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG
    ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG
    GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC
    GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG
    GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG
    ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG
    GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG
    GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC
    CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC
    TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG
    GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC
    GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA
    CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG
    CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC
    TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC
    CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC
    GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG
    CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC
    TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC
    AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG
    CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC
    CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT
    CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA
    TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG
    AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG
    AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC
    AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC
    AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA
    AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA
    AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT
    GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG
    GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC
    GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG
    CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT
    CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT
    TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC
    TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA
    ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG
    TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA
    ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT
    TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC
    GCCGCGATGG GGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846032.1
    CDS277..9354
    Translation

    Target ORF information:

    RefSeq Version XM_024990264.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGCA CAAGACTCTG CTGACATTAT TTTCCTTATT
    GATGGATCAA ACAACACCGG AAGTGTCAAT TTCGCAGTCA TTCTCGACTT CCTTGTAAAT
    CTCCTTGAGA GACTGTCAAT TGGAACTCAG CAGATCCGAG TGGGGGTGGT GCAGTATAGC
    GATGAGCCCA GAACCATGTT CTCCTTGAAC AGCTACTCCA CCAAGGCTCA GGTTCTGGAT
    GCAGTGAAGG CCCTGGGGTT CATTGGTGGA GAGCTGGCCA ATGTTGGCCT TGCCCTGGAT
    TTTGTGGTGG AGAATCACTT CACCCGGGCG GGGGGCAGCC GAGCAGAGGA AGGGGTTCCC
    CAGGTGCTGG TCCTCATAAG TGCTGGGCCT TCTAGTGATG AGATCCGAGA CGGGGTCGTA
    GCACTGAAGC AGGCTAGCGT TTTCTCGTTT GGCCTCGGAG CCCAGGCCGC CTCCAAGGCA
    GAGCTTCAGC ACATAGCTAC CAATGACAAC CTGGTGTTTA CTGTCCCGGA ATTCCGTAGC
    CTTGGGGACG TCCAGGAACA ATTACTGCCG TACATTGTTG GCGTGGCCCA AAGGCACATT
    GTCTTGCAAC CGCCAACCAT TGTCACCCAA GTCATTGAAG TCAACAAGAG GGACATAGTT
    TTCCTGGTGG ATGGCTCTTC CAAACTGGGA CTGACCAACT TCAATGCAAT CCGCGACTTC
    ATTGCCAAGG TCATCCAGAG GCTGGAGATC AGACAGGATC TTATCCAGGT GGCAGTGGCT
    CAGTATGCAG ACACAGTGAG GCCAGAGTTT TATTTCAATA CCTACCCCAG CAAGAGGGAG
    GTCATAAACG CCGTGCGGAA AATGAAGGCT TTGGATGGCT CAGCCTTGTA CACGGGCTCC
    GCTCTGGATT TTGTTCGGAA CAACCTGTTC ACGGAGGCGG CCGGCTACCG GGCTGCCGAG
    GGGGTCCCTA AGCTTCTGGT GCTGGTTACA GGCGGTAAGT CCCTAGATGC GGTCAGCCAG
    CCCGCCCAGG AGCTGAAGAG GAGCGGCATC CTGGCCTTCG CCGTCGGGAA CAAGGTGGCT
    GACCAGGCTG AACTGGAAGA GATCGCTTTC GATTCCTCCC TGGTGTTCAC TGCCACCGAG
    TTCCGACCCG CCCCTTTGCA GGGCGTGCTG CCCGGCTTGC TCGGGCCTCT CAGGACCCTC
    ACTGGAACCA CTGAAGTTCG CGTAAACAAA AGGGATATCA TCTTTCTTTT GGATGGATCG
    TCCAACGTCG GAGAGACCAA TTTCCCTTAT GTGCGGGACT TTGTCATGAA CCTAGTTAAC
    AGCCTTGATG TTGGAAGCGA CCACATTCGG GTCGGTCTAG TGCAATTTAG TGACACTCCG
    GTGACGGAGT TCTCCCTAAA CACCTACCCG ACCAAGTCAG AGTTGCTAGC TCACCTGAGG
    CAGATGCAGC TCCAGGGGGG GTCGGTCCTG AACACGGGCG CGGCCCTGAG CTATGTGCAT
    GCCAACCACT TCACCGAAGC TGGCGGCAGC AGGATCCAGG ACCACGTGCC GCAGCTGCTA
    CTCCTGCTCA CGGCCGGGCA GTCTGAGGAC TCCTATTTGC AAGCCGCCAA CGCCCTGGCG
    CGCGCGGGCA TCCTGACCTT TTGTGTGGGC ACTAGCCAGG CCGATAGGGC GGAGCTTGAG
    GAGATTGCTT TTAACCCGGG CCTGGTGTAT CTCATGGATG ATTTCAGCTC CTTGCCAGCT
    TTGCCTCAAC AGCTGATTCA GCCCCTAACC ACTTATGTTA GCGGAGGTGT GGAGGAGGTG
    CCTCTTGCCC AGCCAGAGAG CAAGCGAGAC ATCCTGTTCC TTTTCGACGG CTCAGCCAAC
    CTCATGGGCC AGTTCACTGC CGCCCGTGAC TTTCTCTACA AGGTCATCGA CGAGCTGGAT
    GTGAAACCTG AGGGGACCCG GGTTGCAGTG GCTCAGTTCA GTGATGATGT CAAGGTGGAG
    TCCCGTTTTG ACCAGCACCA GAATAAGCCT GAGATCCTGA ATCTCGTGAA GAGGATGAAG
    CTCAAGACAG GCAAAGCCCT CAACCTGGGC TACGCCTTGG ATTACGCACA GAGGTACATC
    TTTGTGAAGT CCGCTGGAAG CCGGATTGAG GATGGAGTGC TTCAGTTCCT GGTGCTGCTG
    GTGGCGGGAA AGTCATCGGA CCGGGTGGAT ACACCCGCAC TCAACCTGAA GCAGAGCGGG
    GTGGTGCCCT TCATCCTCCA GGCGAAAAAC GCGGACCCTG CTGAGTTGGA GCTGATCGTA
    CCATCCCCAG CCTTTATTTT GGTTGCGGAG TCCCTTCCTA AGATTGGAGA CCTGCAACCA
    CAGATTGTGA ACCTCTTAAA ATCAGTGCAA AATGGGGCAC CGGCACCAGT TTCAGTTGAA
    AAGGATGTGG TGTTCCTGAT TGATGGCTCC GAGGGTGTCA GGAGCGGCTT CCCACTGCTG
    AAGGAGTTCG TCCAGAGAGT GGTGGAGAGC CTGGACGTGG GACCAGACCG GGTGCGTGTG
    GCCGTGGTGC AGTACAGCGA CCGGACCAGA CCTGAGTTCT ACCTGAATTC CTACATGGAC
    CAGCAGAGTG TGGTCGGAGC CATCCGTGGG CTGACCCTGC TGGGCGGGCC GGCCCCCAAC
    ACAGGGGCTG CCCTGGAGTT CGTCCTGAGG AACATCCTGG TGGGCTCTGC TGGGAGCCGC
    ATAGCAGAAG GTGTCCCCCA GCTCCTGATT GTCCTCACGG CCGACAGGTC TGGGGATGAC
    GTGAGGGGCC CCTCTGTGGT CCTGAGGAGG GGAGGGGCCG TGCCCATCGG CATCGGCATT
    GGGAACGCTG ACATCACCGA GATGCAGACC CTCTCCTTCG TCCCGGACTT CGCCGTGGTC
    ATCCCCACCT TCCGGCAGCT GGGGACCATC CAACAGGTCA TCTCGGAGAG AGTGACCCAG
    CTCAGCCGCG AGGAACTGAG CAGGCTGCAG GCGTCTGTCA CCCCCTTGAC GACTCCGGTG
    GTCAGCAGCA AGAGGGACGT GGTCTTTCTC ATCGATGGGT CCCAGAGTGC TGGCCCCGAG
    TTCCAGTACA TCCGAACGCT CATCGAGAGG CTGGTGGACT ACCTGGATGT GGGCTTCGAC
    ACGACCCGGG TGGCGGTCAT CCAGTTCAGC GATGACCCCA GGGTGGAGTT CCTGTTGAAC
    GTCCACTCCA GCAAGGACGA GGTGCAGAAC GCCGTGCGGC GGCTGCGGCC CAAAGGTGGG
    AGGCAGGTCA ACGTTGGGGG TGCCCTGGAG TACGTGGCCA GGAACATCTT CAAGAGGCCC
    CTGGGGAGCC GGATTGAGGA GGGTGTCCCT CAGTTCCTGG TCCTCATTTC CTCCGGGAAG
    TCGGATGATG AGGTGGAGGA CTCGGCCATA GAGCTCAAGC AGTTTGGCGT GGCGCCACTC
    ACGATCGCCA GGAACGTGGA CCAGGAAGAG CTGGTGAAGA TCTCGCTGAG CCCCGAGTAC
    GTGTTCTCAG TGAACACCTT CCGGGAGCTC CCCAGCCTGG AGCAGAAACT GCTGACACCG
    CTCACCACCC TGACTGCCGG GCAGATACAG CAGCTCCTGG CCAGCACGCG CTACCCGCCT
    CCAGCTGTGG AGAGCGATGC TGCGGACATT GTCTTCCTCA TCGACAGCTC TGATGGCGTG
    AAGCCGGATG GCATCGCACA CATCCGGGAC TTTGTTATCA GGATCGTGCG GAGACTCAAC
    GTCGGTCCCA ACAAAGTGAG AATTGGGGTC CTGCAGTTCA GCAACGACGT CTTCCCAGAA
    TTTCAACTAA AGACCTATAA ATCCCAGGCC AGCGTGCTGG ATGCCATACG TCGCCTGAGG
    TTCAAAGGGG GGTCTCCTCT CAACACTGGC AAGGCTCTGG AATTCGTGGC GAGGAATTAC
    TTTGTCAAGT CTGCCGGGAG CCGGATAGAA GATGGGGTGC CCCAACACCT GGTTCTATTT
    CTGGGTGGAA AATCCCAGGA TGATATTTCC AGGTATTCCC AGGTGATAAA ATCTGCAGGA
    ATTGCGAGCT TAGGGGTTGG AGACCGAAAT ATTGACAGAA CAGAGCTGCA GACCATCACC
    AGCGATCCCA GGCTTGTCTT CACGGTGCGG GAGTTCAGAG ACCTCCCCAG CATAGAAGAA
    AGGATGGTGA ATTCCTTTGG GTCCTCCGGG GTCACCCCTG CACCTCCGGG AGTGGACACA
    CCCTCTCCTT CAAGGCCAGA GAAGAAGAAA GCCGACATTG TGTTCCTGCT GGATGGTTCC
    ATCAATTTCC GGAGAGACAG TTTCCAGGAA GTGCTCCGTT TTGTGTCAGA AATTGTGGAC
    ACGGTTTATG AAGGGGGCGA CTCCATCCAG GTGGGGCTGG TCCAATACAA CTCTGACCCC
    ACCGACGAGT TCTTCCTGAA GGACTTCCCC ACCAAGCAGC AGATTATCGA CGCCATAAAC
    AAGGTGGTCT ACAAAGGGGG GAGGCACGCG AACACCAAGG TGGGCCTGGA GCACCTGCGG
    CGGAACCACT TCGTGCCCGA GGCGGGCAGC CGCCTGGACC AGAGGGTCCC GCAGATCGCC
    TTTGTGATCA CGGGGGGCAA GTCGGTGGAG GACGCTCAGG AGGCAAGCAT GGCGCTCACG
    CAGAGAGGCG TCAAGGTGTT TGCGGTGGGC GTAAGGAACA TCGACTCGGA GGAGGTTGGG
    AAGATAGCGT CCAACAGCGC CACGGCCTTC CGGGTGGGGA ACGTCCAGGA GCTGTCGGAA
    CTGAGCGAGC AGGTTTTGGA AACTCTGCAC GATGCAATGC ATGAGACCTT ATGTCCAGGC
    GTGACTGACG TTTCCAAAGC CTGTAACTTG GATGTGATTC TGGGCTTCGA TGGTTCAGGA
    GATCAGAATG TCTTTGTGGC TCAGAAGGGC CTCGAGCCCA AGGTGGACAC CATCCTGAGG
    AGAATCAGTC AGATGCAGAA GATCAGCTGC AGCGGCAGCC AGCTGCCCAC CGTGCGGGTG
    TCTGTGGTGG CCCTCACGCC CTCGGGCCCC GTTGAGGCCT TTGACTTTGC TGAGTACCAG
    CCGGAGCTGT TTGAGAAGTT CCAGAACATG CGTGCCCAGC ACCCCTACGT CCTCACTGCG
    GACACACTGA AGCTCTACCA GAACAAGTTC CGGCAGGCCT CGATGGACAA CGTGAAGGTG
    GTCATTCATT TTACCGATGG AGTGGATGGG GATCTGGCTG ATTTACAAAG AGCATCAGAG
    GAACTCCGAC AAGAAGGTGT CCGAGCTCTG ATCTTGGTGG GCCTCGAACG CGTCGCCAAC
    TTGGAGCAGC TCATGCAGCT GGAGTTCGGG CGAGGGTTCA CGTACAACCG GCCCCTGAGG
    CTGAACTTGC TGGACCTGGA CTACGAACTA GCCGAGCAGC TTGACAACAT TGCTGAGAAA
    GCCTGCTGTG GGATTCCCTG CAAATGCTCT GGACAAAGAG GAGACCGAGG GCCCATAGGC
    AGCATCGGGC CAAAGGGCAT TCCTGGAGAA GATGGCTACC GAGGTTATCC TGGTGACGAA
    GGTGGACCTG GTGAACGCGG TCCTCCTGGC GTGAACGGCA CTCAAGGTTT CCAGGGCTGC
    CCCGGGCAGA GAGGAGTCAA GGGCTCTCGT GGATTCCCGG GAGAGAAGGG TGAATTAGGA
    GAAATTGGAC TGGATGGTCT TGATGGTGAA GATGGCGACA AAGGGCTGCC CGGTACTTCT
    GGAGAGAAAG GGAGTCCAGG AAGGAGGGGC GACAAAGGAC CCAAAGGCGA CAAAGGGGAG
    AGAGGCGATG TCGGGATCAG AGGCGACCCG GGCAATTCAG GACAGGACAG CCAGCAGAGA
    GGAGCCAAAG GAGAAACCGG AGACATCGGC CCCGTGGGTC TCCCTGGGAG AGACGGGGTG
    TCTGGAGGGC CTGGAGAACC CGGGAAGAGC GGTGGCTTTG GCCGAAGGGG ACCAGCGGGA
    GCTAAGGGCA ACCAGGGCGG CCCCGGCCAG CAGGGCCCTG TAGGAGAGCA GGGGACCAGA
    GGCGCACAGG GTCCGCCTGG TCCCACCGGT CCTCCAGGCC TGATTGGTGA ACAGGGCATT
    CCCGGACCTC GGGGAGGCGG GGGAACCGCA GGTGCTCCTG GAGAACGCGG CAGAACCGGG
    CCCCTGGGAA GAAAGGGTGA GCCCGGAGAT CCAGGACCTA AGGGGAGCAT CGGGAACCGG
    GGTCCTCGTG GGGAGACGGG AGATGATGGG CGAGATGGAG TTGGCAGCGA AGGTCGCATA
    GGCAAGAAGG GAGAAAGAGG ATTCCCTGGA TACCCAGGTC CAAAGGGCAA TCCTGGTGAG
    CCAGGGACAG ACGGACCACC GGGACCCAAA GGCATCAGAG GCCGAAGGGG AAACTCGGGA
    CCTCCAGGGA TAGCTGGACA GAAGGGAGAC CCCGGTTACC CAGGGCCATC TGGTTACAAA
    GGCAGCAGAG GCGACTCAAT GGACCAATGT GCGCTGGTCC AGAGCATCAA AGATAAATGC
    CCTTGCTGTT ACGGGCCCCT GGAGTGCCCC GTCTTCCCGA CGGAGCTGGC TTTCGCCTTG
    GACACCTCAG AGGGGGTCAC CCAGGACAGG TTCAGCCAGA TGAGGGAGGT GGTCCTCAAG
    ATTCTGGACG ACCTGACCAT CGCCGAGAGC AACTGCCCGA GGGGGGCCCG CGTGGCCGTG
    GTCACTTACA ACAACGAGGT TACCACGGAG ATCCGCTTTG CCGACTCCAA GAAGAAGTCC
    GTCCTCCTGG ACAAGATCAA GAACCTGCAG GTGTCGCTGA CCTCCAAGCA GCAGAGCCTG
    GAAACCGCCA TGTCCTTTGT GGCCAGAAAC ACGTTTAAGC GTGTGCGGAG CGGGTTCCTG
    ATGAGGAAGG TGGCGGTGTT CTTCAGCAAC AAGCCCACGA GGGCGACCCC GCAGCTCCGG
    GAGGCTGTGC TCAAGCTCTC AGATGCTGGG ATCACACCCT TGTTCCTCAC AAGCCAGGAG
    GACCGGCAGC TGATCAATGC CCTACAGATC AATAACACAG CGGTGGGACA TGCCCTTGTC
    CTTCCTGCTG GCGGAGACCT CACAGACTTC TTGAAGAATG TCCTGACGTG TCACGTCTGC
    TTGGACATCT GCAACATTGA CCCATCCTGT GGATTCGGCA GCTGGAGGCC TTCCTTCAGG
    GACCGGAGGG CAGCAGGCAG TGATGTGGAC ATCGACATGG CTTTCATCTT AGATAGCTCC
    GAGTCCACCA CCCCGTTCCA GTTCAACGAG ATGAGGAAGT ACATCGAGTA CCTGGTCCGA
    CAGCTGGATG TGAGCCCAGA CCCCAAGGCC TCCCAGCACT TTGCCCGCGT GGCGGTGGTG
    CAGCATGCGC CCTACGAGGC GGTGGACAAC GCCAGTGTGC CGCCTGTGAA GGTGGAGTTC
    TCCCTGACCG ACTACGGCTC CAAAGAGAAG CTGCTGGCCT TCCTTGGCAG CAGGATGACC
    CAGCTGCAGG GGACCAGGGC TTTGGGCCGC GCCATCGACT ACACCATTGA GAACATCTTC
    GAAAGTGCTC CCAACCCCCG GGACCTGAAG ATCGTGGTGC TGATGCTGAC GGGCGAGGTG
    CAGAAGCAGC AGCTGGAGGA GGCCCAGAGA GCCATCCTAC AGGCCAAGTG CAAAGGCTAC
    TTCTTCGTGA TCCTGGGCAT TGGCAGGAAG GTGAATGTCA AGGAGCTGTA CAGCTCTGCC
    AGCGAGCCCA ACGACGTCTT CTTCAAGCTA GTGGACAAGT CGACCGAGCT CAACGAGGAG
    CCCCTAATGC GCTTCGGGAG GCTGCTGCCA TCCTTCGTCA GCAGTAAAGA TGCTTTCTAC
    CTGTCCCCAG ATATCAGGAA ACAGTGTGAT TGGTTCCAAG CAGACCAACC GGCAAAGAAT
    CTCGTGCAGT TTGGTTACAA ACAAATAAAT GTTCCTAATA ACGTTACTTC AAGTCCTACA
    TCTAAACTGG TGACCACAAC GAAGCCGGTG ATCACCACCA CCAAACCGAT CACAGTGGTG
    AACCTGCCAG CCTCAAAGCC AGCTGCCACG AGACCTGTGG ATGAGAGACC CGCAGCTGGG
    AGATCCGTGG CCACCAAGAT GGACAGTGCC AGGCCCGTGG CCACCAAGAC CGAGGCCACC
    AAACCCGTGG CCACCAAGAC CGAGGCCACC AAGACTGAAG CTGCCAAACC TGTGGTCACC
    AAGGCTGAGG CTGCAAAACC CACGGCCACC AAGACTGAAG CCACTAAGAC CGAGGCTGCA
    AAGCTCATGG CCACCAAGAC CGAGGCCACC AAACCTGTGG CTGCCAGGCC CGAGGCGGCA
    AAGACAGCCA CAGCCAGACC TGCAGCAGCC GCGAAGCCTG CAGCAGCCAA ACCTGCCCCT
    GCCAGACCCC CCACTGCTGC CAGATCCATG GCCACGAGGT CCGAGGCCCC CAGGCCCCAG
    GCAGTCAAAC TGGCTGCCAG CAGGCCCGGC GCTGCTAAGC CCGTGGTGAA GGCACCTCGC
    GAGATCCATG TGTCTGAGGT CACAGAGAAC AGCGCCAAGC TGCACTGGGA GAGGCCCGAG
    CCCCCCAGCC CTTACTTATA CAACCTCACC GTCACCTCGG CCCACGATCA GGCCGTGGTT
    CTGAAGCAGA ACCTCACTGT CACGGACCGC GTCATCGGGG GCCTGCTCCC CGGGCAGACT
    TACCACGTTA CGGTGACCTG CTCCCTGAGG TCTCAGGTCA GAGCCATCTA CCAAGGCAGC
    TTCAGCACGA AGAAAATTCA GCCTCCACCT CCACAAACAG AGCGATCAGC CTCTAGTGCA
    ACCATCAACC TAGTGGTGAG CGCAGACCGG TTGGCTGGGA GTAAAGCAGA TATATGTAAG
    TTGCCAAAGG ACGGCGGAAC TTGCAGAGAG TTTGTATTAA AGTGGTACTA TGATTCCGTA
    ACTGAAAACT GCGCCCGATT CTGGTATGGA GGCTGCGGTG GAAACGAAAA CAGATTTAAT
    TCGCAGGATG AATGTGAAAA GGTTTGCCCT CCTGTGCCCA TCAAGCAGCC CGGAGTTATC
    GCCGCGATGG GGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51858
    Clone ID Related Accession (Same CDS sequence) XM_010803869.2 , XM_010827157.2 , XM_024990266.1
    Accession Version XM_010803869.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802171.1
    CDS340..8214
    Translation

    Target ORF information:

    RefSeq Version XM_010803869.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803869.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51858
    Clone ID Related Accession (Same CDS sequence) XM_010803869.2 , XM_010827157.2 , XM_024990266.1
    Accession Version XM_010827157.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825459.1
    CDS340..8214
    Translation

    Target ORF information:

    RefSeq Version XM_010827157.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010827157.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51858
    Clone ID Related Accession (Same CDS sequence) XM_010803869.2 , XM_010827157.2 , XM_024990266.1
    Accession Version XM_024990266.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846034.1
    CDS276..8150
    Translation

    Target ORF information:

    RefSeq Version XM_024990266.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGTC ATTGAAGTCA ACAAGAGGGA CATAGTTTTC
    CTGGTGGATG GCTCTTCCAA ACTGGGACTG ACCAACTTCA ATGCAATCCG CGACTTCATT
    GCCAAGGTCA TCCAGAGGCT GGAGATCAGA CAGGATCTTA TCCAGGTGGC AGTGGCTCAG
    TATGCAGACA CAGTGAGGCC AGAGTTTTAT TTCAATACCT ACCCCAGCAA GAGGGAGGTC
    ATAAACGCCG TGCGGAAAAT GAAGGCTTTG GATGGCTCAG CCTTGTACAC GGGCTCCGCT
    CTGGATTTTG TTCGGAACAA CCTGTTCACG GAGGCGGCCG GCTACCGGGC TGCCGAGGGG
    GTCCCTAAGC TTCTGGTGCT GGTTACAGGC GGTAAGTCCC TAGATGCGGT CAGCCAGCCC
    GCCCAGGAGC TGAAGAGGAG CGGCATCCTG GCCTTCGCCG TCGGGAACAA GGTGGCTGAC
    CAGGCTGAAC TGGAAGAGAT CGCTTTCGAT TCCTCCCTGG TGTTCACTGC CACCGAGTTC
    CGACCCGCCC CTTTGCAGGG CGTGCTGCCC GGCTTGCTCG GGCCTCTCAG GACCCTCACT
    GGAACCACTG AAGAGAGCAA GCGAGACATC CTGTTCCTTT TCGACGGCTC AGCCAACCTC
    ATGGGCCAGT TCACTGCCGC CCGTGACTTT CTCTACAAGG TCATCGACGA GCTGGATGTG
    AAACCTGAGG GGACCCGGGT TGCAGTGGCT CAGTTCAGTG ATGATGTCAA GGTGGAGTCC
    CGTTTTGACC AGCACCAGAA TAAGCCTGAG ATCCTGAATC TCGTGAAGAG GATGAAGCTC
    AAGACAGGCA AAGCCCTCAA CCTGGGCTAC GCCTTGGATT ACGCACAGAG GTACATCTTT
    GTGAAGTCCG CTGGAAGCCG GATTGAGGAT GGAGTGCTTC AGTTCCTGGT GCTGCTGGTG
    GCGGGAAAGT CATCGGACCG GGTGGATACA CCCGCACTCA ACCTGAAGCA GAGCGGGGTG
    GTGCCCTTCA TCCTCCAGGC GAAAAACGCG GACCCTGCTG AGTTGGAGCT GATCGTACCA
    TCCCCAGCCT TTATTTTGGT TGCGGAGTCC CTTCCTAAGA TTGGAGACCT GCAACCACAG
    ATTGTGAACC TCTTAAAATC AGTGCAAAAT GGGGCACCGG CACCAGTTTC AGTTGAAAAG
    GATGTGGTGT TCCTGATTGA TGGCTCCGAG GGTGTCAGGA GCGGCTTCCC ACTGCTGAAG
    GAGTTCGTCC AGAGAGTGGT GGAGAGCCTG GACGTGGGAC CAGACCGGGT GCGTGTGGCC
    GTGGTGCAGT ACAGCGACCG GACCAGACCT GAGTTCTACC TGAATTCCTA CATGGACCAG
    CAGAGTGTGG TCGGAGCCAT CCGTGGGCTG ACCCTGCTGG GCGGGCCGGC CCCCAACACA
    GGGGCTGCCC TGGAGTTCGT CCTGAGGAAC ATCCTGGTGG GCTCTGCTGG GAGCCGCATA
    GCAGAAGGTG TCCCCCAGCT CCTGATTGTC CTCACGGCCG ACAGGTCTGG GGATGACGTG
    AGGGGCCCCT CTGTGGTCCT GAGGAGGGGA GGGGCCGTGC CCATCGGCAT CGGCATTGGG
    AACGCTGACA TCACCGAGAT GCAGACCCTC TCCTTCGTCC CGGACTTCGC CGTGGTCATC
    CCCACCTTCC GGCAGCTGGG GACCATCCAA CAGGTCATCT CGGAGAGAGT GACCCAGCTC
    AGCCGCGAGG AACTGAGCAG GCTGCAGGCG TCTGTCACCC CCTTGACGAC TCCGGTGGTC
    AGCAGCAAGA GGGACGTGGT CTTTCTCATC GATGGGTCCC AGAGTGCTGG CCCCGAGTTC
    CAGTACATCC GAACGCTCAT CGAGAGGCTG GTGGACTACC TGGATGTGGG CTTCGACACG
    ACCCGGGTGG CGGTCATCCA GTTCAGCGAT GACCCCAGGG TGGAGTTCCT GTTGAACGTC
    CACTCCAGCA AGGACGAGGT GCAGAACGCC GTGCGGCGGC TGCGGCCCAA AGGTGGGAGG
    CAGGTCAACG TTGGGGGTGC CCTGGAGTAC GTGGCCAGGA ACATCTTCAA GAGGCCCCTG
    GGGAGCCGGA TTGAGGAGGG TGTCCCTCAG TTCCTGGTCC TCATTTCCTC CGGGAAGTCG
    GATGATGAGG TGGAGGACTC GGCCATAGAG CTCAAGCAGT TTGGCGTGGC GCCACTCACG
    ATCGCCAGGA ACGTGGACCA GGAAGAGCTG GTGAAGATCT CGCTGAGCCC CGAGTACGTG
    TTCTCAGTGA ACACCTTCCG GGAGCTCCCC AGCCTGGAGC AGAAACTGCT GACACCGCTC
    ACCACCCTGA CTGCCGGGCA GATACAGCAG CTCCTGGCCA GCACGCGCTA CCCGCCTCCA
    GCTGTGGAGA GCGATGCTGC GGACATTGTC TTCCTCATCG ACAGCTCTGA TGGCGTGAAG
    CCGGATGGCA TCGCACACAT CCGGGACTTT GTTATCAGGA TCGTGCGGAG ACTCAACGTC
    GGTCCCAACA AAGTGAGAAT TGGGGTCCTG CAGTTCAGCA ACGACGTCTT CCCAGAATTT
    CAACTAAAGA CCTATAAATC CCAGGCCAGC GTGCTGGATG CCATACGTCG CCTGAGGTTC
    AAAGGGGGGT CTCCTCTCAA CACTGGCAAG GCTCTGGAAT TCGTGGCGAG GAATTACTTT
    GTCAAGTCTG CCGGGAGCCG GATAGAAGAT GGGGTGCCCC AACACCTGGT TCTATTTCTG
    GGTGGAAAAT CCCAGGATGA TATTTCCAGG TATTCCCAGG TGATAAAATC TGCAGGAATT
    GCGAGCTTAG GGGTTGGAGA CCGAAATATT GACAGAACAG AGCTGCAGAC CATCACCAGC
    GATCCCAGGC TTGTCTTCAC GGTGCGGGAG TTCAGAGACC TCCCCAGCAT AGAAGAAAGG
    ATGGTGAATT CCTTTGGGTC CTCCGGGGTC ACCCCTGCAC CTCCGGGAGT GGACACACCC
    TCTCCTTCAA GGCCAGAGAA GAAGAAAGCC GACATTGTGT TCCTGCTGGA TGGTTCCATC
    AATTTCCGGA GAGACAGTTT CCAGGAAGTG CTCCGTTTTG TGTCAGAAAT TGTGGACACG
    GTTTATGAAG GGGGCGACTC CATCCAGGTG GGGCTGGTCC AATACAACTC TGACCCCACC
    GACGAGTTCT TCCTGAAGGA CTTCCCCACC AAGCAGCAGA TTATCGACGC CATAAACAAG
    GTGGTCTACA AAGGGGGGAG GCACGCGAAC ACCAAGGTGG GCCTGGAGCA CCTGCGGCGG
    AACCACTTCG TGCCCGAGGC GGGCAGCCGC CTGGACCAGA GGGTCCCGCA GATCGCCTTT
    GTGATCACGG GGGGCAAGTC GGTGGAGGAC GCTCAGGAGG CAAGCATGGC GCTCACGCAG
    AGAGGCGTCA AGGTGTTTGC GGTGGGCGTA AGGAACATCG ACTCGGAGGA GGTTGGGAAG
    ATAGCGTCCA ACAGCGCCAC GGCCTTCCGG GTGGGGAACG TCCAGGAGCT GTCGGAACTG
    AGCGAGCAGG TTTTGGAAAC TCTGCACGAT GCAATGCATG AGACCTTATG TCCAGGCGTG
    ACTGACGTTT CCAAAGCCTG TAACTTGGAT GTGATTCTGG GCTTCGATGG TTCAGGAGAT
    CAGAATGTCT TTGTGGCTCA GAAGGGCCTC GAGCCCAAGG TGGACACCAT CCTGAGGAGA
    ATCAGTCAGA TGCAGAAGAT CAGCTGCAGC GGCAGCCAGC TGCCCACCGT GCGGGTGTCT
    GTGGTGGCCC TCACGCCCTC GGGCCCCGTT GAGGCCTTTG ACTTTGCTGA GTACCAGCCG
    GAGCTGTTTG AGAAGTTCCA GAACATGCGT GCCCAGCACC CCTACGTCCT CACTGCGGAC
    ACACTGAAGC TCTACCAGAA CAAGTTCCGG CAGGCCTCGA TGGACAACGT GAAGGTGGTC
    ATTCATTTTA CCGATGGAGT GGATGGGGAT CTGGCTGATT TACAAAGAGC ATCAGAGGAA
    CTCCGACAAG AAGGTGTCCG AGCTCTGATC TTGGTGGGCC TCGAACGCGT CGCCAACTTG
    GAGCAGCTCA TGCAGCTGGA GTTCGGGCGA GGGTTCACGT ACAACCGGCC CCTGAGGCTG
    AACTTGCTGG ACCTGGACTA CGAACTAGCC GAGCAGCTTG ACAACATTGC TGAGAAAGCC
    TGCTGTGGGA TTCCCTGCAA ATGCTCTGGA CAAAGAGGAG ACCGAGGGCC CATAGGCAGC
    ATCGGGCCAA AGGGCATTCC TGGAGAAGAT GGCTACCGAG GTTATCCTGG TGACGAAGGT
    GGACCTGGTG AACGCGGTCC TCCTGGCGTG AACGGCACTC AAGGTTTCCA GGGCTGCCCC
    GGGCAGAGAG GAGTCAAGGG CTCTCGTGGA TTCCCGGGAG AGAAGGGTGA ATTAGGAGAA
    ATTGGACTGG ATGGTCTTGA TGGTGAAGAT GGCGACAAAG GGCTGCCCGG TACTTCTGGA
    GAGAAAGGGA GTCCAGGAAG GAGGGGCGAC AAAGGACCCA AAGGCGACAA AGGGGAGAGA
    GGCGATGTCG GGATCAGAGG CGACCCGGGC AATTCAGGAC AGGACAGCCA GCAGAGAGGA
    GCCAAAGGAG AAACCGGAGA CATCGGCCCC GTGGGTCTCC CTGGGAGAGA CGGGGTGTCT
    GGAGGGCCTG GAGAACCCGG GAAGAGCGGT GGCTTTGGCC GAAGGGGACC AGCGGGAGCT
    AAGGGCAACC AGGGCGGCCC CGGCCAGCAG GGCCCTGTAG GAGAGCAGGG GACCAGAGGC
    GCACAGGGTC CGCCTGGTCC CACCGGTCCT CCAGGCCTGA TTGGTGAACA GGGCATTCCC
    GGACCTCGGG GAGGCGGGGG AACCGCAGGT GCTCCTGGAG AACGCGGCAG AACCGGGCCC
    CTGGGAAGAA AGGGTGAGCC CGGAGATCCA GGACCTAAGG GGAGCATCGG GAACCGGGGT
    CCTCGTGGGG AGACGGGAGA TGATGGGCGA GATGGAGTTG GCAGCGAAGG TCGCATAGGC
    AAGAAGGGAG AAAGAGGATT CCCTGGATAC CCAGGTCCAA AGGGCAATCC TGGTGAGCCA
    GGGACAGACG GACCACCGGG ACCCAAAGGC ATCAGAGGCC GAAGGGGAAA CTCGGGACCT
    CCAGGGATAG CTGGACAGAA GGGAGACCCC GGTTACCCAG GGCCATCTGG TTACAAAGGC
    AGCAGAGGCG ACTCAATGGA CCAATGTGCG CTGGTCCAGA GCATCAAAGA TAAATGCCCT
    TGCTGTTACG GGCCCCTGGA GTGCCCCGTC TTCCCGACGG AGCTGGCTTT CGCCTTGGAC
    ACCTCAGAGG GGGTCACCCA GGACAGGTTC AGCCAGATGA GGGAGGTGGT CCTCAAGATT
    CTGGACGACC TGACCATCGC CGAGAGCAAC TGCCCGAGGG GGGCCCGCGT GGCCGTGGTC
    ACTTACAACA ACGAGGTTAC CACGGAGATC CGCTTTGCCG ACTCCAAGAA GAAGTCCGTC
    CTCCTGGACA AGATCAAGAA CCTGCAGGTG TCGCTGACCT CCAAGCAGCA GAGCCTGGAA
    ACCGCCATGT CCTTTGTGGC CAGAAACACG TTTAAGCGTG TGCGGAGCGG GTTCCTGATG
    AGGAAGGTGG CGGTGTTCTT CAGCAACAAG CCCACGAGGG CGACCCCGCA GCTCCGGGAG
    GCTGTGCTCA AGCTCTCAGA TGCTGGGATC ACACCCTTGT TCCTCACAAG CCAGGAGGAC
    CGGCAGCTGA TCAATGCCCT ACAGATCAAT AACACAGCGG TGGGACATGC CCTTGTCCTT
    CCTGCTGGCG GAGACCTCAC AGACTTCTTG AAGAATGTCC TGACGTGTCA CGTCTGCTTG
    GACATCTGCA ACATTGACCC ATCCTGTGGA TTCGGCAGCT GGAGGCCTTC CTTCAGGGAC
    CGGAGGGCAG CAGGCAGTGA TGTGGACATC GACATGGCTT TCATCTTAGA TAGCTCCGAG
    TCCACCACCC CGTTCCAGTT CAACGAGATG AGGAAGTACA TCGAGTACCT GGTCCGACAG
    CTGGATGTGA GCCCAGACCC CAAGGCCTCC CAGCACTTTG CCCGCGTGGC GGTGGTGCAG
    CATGCGCCCT ACGAGGCGGT GGACAACGCC AGTGTGCCGC CTGTGAAGGT GGAGTTCTCC
    CTGACCGACT ACGGCTCCAA AGAGAAGCTG CTGGCCTTCC TTGGCAGCAG GATGACCCAG
    CTGCAGGGGA CCAGGGCTTT GGGCCGCGCC ATCGACTACA CCATTGAGAA CATCTTCGAA
    AGTGCTCCCA ACCCCCGGGA CCTGAAGATC GTGGTGCTGA TGCTGACGGG CGAGGTGCAG
    AAGCAGCAGC TGGAGGAGGC CCAGAGAGCC ATCCTACAGG CCAAGTGCAA AGGCTACTTC
    TTCGTGATCC TGGGCATTGG CAGGAAGGTG AATGTCAAGG AGCTGTACAG CTCTGCCAGC
    GAGCCCAACG ACGTCTTCTT CAAGCTAGTG GACAAGTCGA CCGAGCTCAA CGAGGAGCCC
    CTAATGCGCT TCGGGAGGCT GCTGCCATCC TTCGTCAGCA GTAAAGATGC TTTCTACCTG
    TCCCCAGATA TCAGGAAACA GTGTGATTGG TTCCAAGCAG ACCAACCGGC AAAGAATCTC
    GTGCAGTTTG GTTACAAACA AATAAATGTT CCTAATAACG TTACTTCAAG TCCTACATCT
    AAACTGGTGA CCACAACGAA GCCGGTGATC ACCACCACCA AACCGATCAC AGTGGTGAAC
    CTGCCAGCCT CAAAGCCAGC TGCCACGAGA CCTGTGGATG AGAGACCCGC AGCTGGGAGA
    TCCGTGGCCA CCAAGATGGA CAGTGCCAGG CCCGTGGCCA CCAAGACCGA GGCCACCAAA
    CCCGTGGCCA CCAAGACCGA GGCCACCAAG ACTGAAGCTG CCAAACCTGT GGTCACCAAG
    GCTGAGGCTG CAAAACCCAC GGCCACCAAG ACTGAAGCCA CTAAGACCGA GGCTGCAAAG
    CTCATGGCCA CCAAGACCGA GGCCACCAAA CCTGTGGCTG CCAGGCCCGA GGCGGCAAAG
    ACAGCCACAG CCAGACCTGC AGCAGCCGCG AAGCCTGCAG CAGCCAAACC TGCCCCTGCC
    AGACCCCCCA CTGCTGCCAG ATCCATGGCC ACGAGGTCCG AGGCCCCCAG GCCCCAGGCA
    GTCAAACTGG CTGCCAGCAG GCCCGGCGCT GCTAAGCCCG TGGTGAAGGC ACCTCGCGAG
    ATCCATGTGT CTGAGGTCAC AGAGAACAGC GCCAAGCTGC ACTGGGAGAG GCCCGAGCCC
    CCCAGCCCTT ACTTATACAA CCTCACCGTC ACCTCGGCCC ACGATCAGGC CGTGGTTCTG
    AAGCAGAACC TCACTGTCAC GGACCGCGTC ATCGGGGGCC TGCTCCCCGG GCAGACTTAC
    CACGTTACGG TGACCTGCTC CCTGAGGTCT CAGGTCAGAG CCATCTACCA AGGCAGCTTC
    AGCACGAAGA AAATTCAGCC TCCACCTCCA CAAACAGAGC GATCAGCCTC TAGTGCAACC
    ATCAACCTAG TGGTGAGCGC AGACCGGTTG GCTGGGAGTA AAGCAGATAT ATGTAAGTTG
    CCAAAGGACG GCGGAACTTG CAGAGAGTTT GTATTAAAGT GGTACTATGA TTCCGTAACT
    GAAAACTGCG CCCGATTCTG GTATGGAGGC TGCGGTGGAA ACGAAAACAG ATTTAATTCG
    CAGGATGAAT GTGAAAAGGT TTGCCCTCCT GTGCCCATCA AGCAGCCCGG AGTTATCGCC
    GCGATGGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51859
    Clone ID Related Accession (Same CDS sequence) XM_010803870.2 , XM_010827158.2 , XM_024990267.1
    Accession Version XM_010803870.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC
    GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC
    GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT
    GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG
    AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA
    CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC
    CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG
    AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG
    GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA
    GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA
    GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC
    TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC
    CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT
    TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG
    CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT
    GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG
    TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC
    GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC
    TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG
    AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG
    ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT
    GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT
    GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG
    TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG
    CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC
    TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT
    TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC
    GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG
    AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA
    CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG
    CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC
    TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG
    CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC
    GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA
    CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG
    GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC
    CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA
    AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG
    CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC
    AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG
    GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG
    CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA
    GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC
    AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC
    GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG
    GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC
    CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC
    ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG
    GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG
    GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC
    TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC
    GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC
    ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC
    ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT
    GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC
    GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC
    AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA
    AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA
    CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC
    CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC
    ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA
    GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT
    CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT
    TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG
    GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG
    CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC
    GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC
    AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG
    AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG
    GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG
    CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT
    GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC
    GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC
    ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC
    GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC
    AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG
    GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA
    TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC
    AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG
    GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG
    GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC
    CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC
    AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG
    CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG
    AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC
    CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC
    ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA
    CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG
    TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG
    CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC
    TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG
    TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC
    GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG
    AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC
    AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT
    GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG
    ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG
    TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG
    TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG
    CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA
    GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA
    CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT
    TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG
    ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA
    CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG
    ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA
    CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG
    ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG
    CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC
    AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC
    CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG
    AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG
    GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT
    CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC
    CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC
    TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA
    GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA
    GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC
    TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA
    AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG
    CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802172.1
    CDS338..7627
    Translation

    Target ORF information:

    RefSeq Version XM_010803870.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803870.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC
    GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC
    GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT
    GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG
    AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA
    CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC
    CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG
    AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG
    GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA
    GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA
    GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC
    TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC
    CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT
    TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG
    CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT
    GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG
    TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC
    GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC
    TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG
    AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG
    ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT
    GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT
    GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG
    TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG
    CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC
    TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT
    TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC
    GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG
    AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA
    CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG
    CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC
    TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG
    CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC
    GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA
    CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG
    GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC
    CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA
    AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG
    CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC
    AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG
    GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG
    CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA
    GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC
    AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC
    GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG
    GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC
    CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC
    ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG
    GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG
    GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC
    TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC
    GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC
    ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC
    ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT
    GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC
    GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC
    AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA
    AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA
    CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC
    CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC
    ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA
    GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT
    CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT
    TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG
    GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG
    CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC
    GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC
    AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG
    AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG
    GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG
    CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT
    GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC
    GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC
    ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC
    GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC
    AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG
    GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA
    TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC
    AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG
    GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG
    GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC
    CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC
    AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG
    CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG
    AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC
    CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC
    ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA
    CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG
    TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG
    CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC
    TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG
    TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC
    GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG
    AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC
    AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT
    GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG
    ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG
    TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG
    TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG
    CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA
    GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA
    CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT
    TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG
    ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA
    CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG
    ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA
    CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG
    ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG
    CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC
    AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC
    CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG
    AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG
    GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT
    CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC
    CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC
    TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA
    GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA
    GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC
    TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA
    AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG
    CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51859
    Clone ID Related Accession (Same CDS sequence) XM_010803870.2 , XM_010827158.2 , XM_024990267.1
    Accession Version XM_010827158.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644673.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC
    GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC
    GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT
    GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG
    AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA
    CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC
    CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG
    AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG
    GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA
    GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA
    GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC
    TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC
    CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT
    TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG
    CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT
    GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG
    TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC
    GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC
    TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG
    AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG
    ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT
    GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT
    GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG
    TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG
    CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC
    TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT
    TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC
    GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG
    AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA
    CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG
    CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC
    TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG
    CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC
    GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA
    CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG
    GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC
    CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA
    AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG
    CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC
    AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG
    GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG
    CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA
    GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC
    AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC
    GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG
    GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC
    CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC
    ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG
    GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG
    GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC
    TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC
    GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC
    ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC
    ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT
    GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC
    GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC
    AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA
    AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA
    CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC
    CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC
    ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA
    GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT
    CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT
    TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG
    GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG
    CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC
    GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC
    AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG
    AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG
    GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG
    CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT
    GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC
    GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC
    ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC
    GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC
    AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG
    GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA
    TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC
    AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG
    GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG
    GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC
    CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC
    AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG
    CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG
    AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC
    CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC
    ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA
    CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG
    TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG
    CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC
    TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG
    TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC
    GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG
    AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC
    AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT
    GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG
    ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG
    TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG
    TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG
    CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA
    GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA
    CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT
    TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG
    ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA
    CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG
    ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA
    CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG
    ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG
    CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC
    AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC
    CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG
    AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG
    GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT
    CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC
    CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC
    TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA
    GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA
    GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC
    TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA
    AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG
    CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825460.1
    CDS338..7627
    Translation

    Target ORF information:

    RefSeq Version XM_010827158.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 (COL6A3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010827158.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC
    GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC
    GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT
    GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG
    AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA
    CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC
    CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG
    AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG
    GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA
    GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA
    GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC
    TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC
    CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT
    TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG
    CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT
    GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG
    TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC
    GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC
    TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG
    AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG
    ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT
    GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT
    GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG
    TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG
    CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC
    TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT
    TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC
    GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG
    AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA
    CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG
    CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC
    TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG
    CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC
    GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA
    CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG
    GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC
    CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA
    AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG
    CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC
    AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG
    GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG
    CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA
    GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC
    AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC
    GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG
    GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC
    CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC
    ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG
    GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG
    GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC
    TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC
    GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC
    ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC
    ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT
    GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC
    GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC
    AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA
    AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA
    CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC
    CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC
    ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA
    GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT
    CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT
    TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG
    GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG
    CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC
    GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC
    AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG
    AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG
    GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG
    CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT
    GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC
    GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC
    ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC
    GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC
    AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG
    GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA
    TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC
    AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG
    GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG
    GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC
    CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC
    AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG
    CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG
    AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC
    CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC
    ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA
    CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG
    TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG
    CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC
    TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG
    TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC
    GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG
    AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC
    AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT
    GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG
    ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG
    TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG
    TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG
    CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA
    GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA
    CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT
    TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG
    ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA
    CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG
    ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA
    CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG
    ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG
    CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC
    AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC
    CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG
    AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG
    GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT
    CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC
    CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC
    TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA
    GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA
    GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC
    TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA
    AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG
    CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51859
    Clone ID Related Accession (Same CDS sequence) XM_010803870.2 , XM_010827158.2 , XM_024990267.1
    Accession Version XM_024990267.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-3(VI) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC
    GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC
    GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT
    GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG
    AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA
    CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC
    CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG
    AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG
    GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA
    GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA
    GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC
    TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC
    CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT
    TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG
    CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT
    GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG
    TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC
    GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC
    TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG
    AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG
    ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT
    GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT
    GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG
    TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG
    CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC
    TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT
    TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC
    GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG
    AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA
    CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG
    CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC
    TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG
    CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC
    GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA
    CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG
    GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC
    CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA
    AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG
    CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC
    AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG
    GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG
    CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA
    GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC
    AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC
    GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG
    GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC
    CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC
    ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG
    GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG
    GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC
    TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC
    GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC
    ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC
    ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT
    GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC
    GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC
    AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA
    AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA
    CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC
    CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC
    ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA
    GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT
    CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT
    TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG
    GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG
    CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC
    GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC
    AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG
    AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG
    GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG
    CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT
    GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC
    GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC
    ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC
    GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC
    AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG
    GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA
    TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC
    AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG
    GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG
    GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC
    CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC
    AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG
    CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG
    AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC
    CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC
    ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA
    CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG
    TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG
    CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC
    TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG
    TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC
    GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG
    AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC
    AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT
    GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG
    ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG
    TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG
    TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG
    CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA
    GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA
    CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT
    TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG
    ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA
    CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG
    ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA
    CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG
    ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG
    CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC
    AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC
    CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG
    AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG
    GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT
    CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC
    CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC
    TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA
    GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA
    GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC
    TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA
    AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG
    CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846035.1
    CDS275..7564
    Translation

    Target ORF information:

    RefSeq Version XM_024990267.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type VI alpha 3 chain (COL6A3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990267.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGGAAGC ATCGGCATTT GCCCTTAGTG GCCATTCTTT GCCTCTTTTT CTCAGGCTTC 
    TCCTTTACTC GTGGTCAACA ACAGCCAGAG AGCAAGCGAG ACATCCTGTT CCTTTTCGAC
    GGCTCAGCCA ACCTCATGGG CCAGTTCACT GCCGCCCGTG ACTTTCTCTA CAAGGTCATC
    GACGAGCTGG ATGTGAAACC TGAGGGGACC CGGGTTGCAG TGGCTCAGTT CAGTGATGAT
    GTCAAGGTGG AGTCCCGTTT TGACCAGCAC CAGAATAAGC CTGAGATCCT GAATCTCGTG
    AAGAGGATGA AGCTCAAGAC AGGCAAAGCC CTCAACCTGG GCTACGCCTT GGATTACGCA
    CAGAGGTACA TCTTTGTGAA GTCCGCTGGA AGCCGGATTG AGGATGGAGT GCTTCAGTTC
    CTGGTGCTGC TGGTGGCGGG AAAGTCATCG GACCGGGTGG ATACACCCGC ACTCAACCTG
    AAGCAGAGCG GGGTGGTGCC CTTCATCCTC CAGGCGAAAA ACGCGGACCC TGCTGAGTTG
    GAGCTGATCG TACCATCCCC AGCCTTTATT TTGGTTGCGG AGTCCCTTCC TAAGATTGGA
    GACCTGCAAC CACAGATTGT GAACCTCTTA AAATCAGTGC AAAATGGGGC ACCGGCACCA
    GTTTCAGTTG AAAAGGATGT GGTGTTCCTG ATTGATGGCT CCGAGGGTGT CAGGAGCGGC
    TTCCCACTGC TGAAGGAGTT CGTCCAGAGA GTGGTGGAGA GCCTGGACGT GGGACCAGAC
    CGGGTGCGTG TGGCCGTGGT GCAGTACAGC GACCGGACCA GACCTGAGTT CTACCTGAAT
    TCCTACATGG ACCAGCAGAG TGTGGTCGGA GCCATCCGTG GGCTGACCCT GCTGGGCGGG
    CCGGCCCCCA ACACAGGGGC TGCCCTGGAG TTCGTCCTGA GGAACATCCT GGTGGGCTCT
    GCTGGGAGCC GCATAGCAGA AGGTGTCCCC CAGCTCCTGA TTGTCCTCAC GGCCGACAGG
    TCTGGGGATG ACGTGAGGGG CCCCTCTGTG GTCCTGAGGA GGGGAGGGGC CGTGCCCATC
    GGCATCGGCA TTGGGAACGC TGACATCACC GAGATGCAGA CCCTCTCCTT CGTCCCGGAC
    TTCGCCGTGG TCATCCCCAC CTTCCGGCAG CTGGGGACCA TCCAACAGGT CATCTCGGAG
    AGAGTGACCC AGCTCAGCCG CGAGGAACTG AGCAGGCTGC AGGCGTCTGT CACCCCCTTG
    ACGACTCCGG TGGTCAGCAG CAAGAGGGAC GTGGTCTTTC TCATCGATGG GTCCCAGAGT
    GCTGGCCCCG AGTTCCAGTA CATCCGAACG CTCATCGAGA GGCTGGTGGA CTACCTGGAT
    GTGGGCTTCG ACACGACCCG GGTGGCGGTC ATCCAGTTCA GCGATGACCC CAGGGTGGAG
    TTCCTGTTGA ACGTCCACTC CAGCAAGGAC GAGGTGCAGA ACGCCGTGCG GCGGCTGCGG
    CCCAAAGGTG GGAGGCAGGT CAACGTTGGG GGTGCCCTGG AGTACGTGGC CAGGAACATC
    TTCAAGAGGC CCCTGGGGAG CCGGATTGAG GAGGGTGTCC CTCAGTTCCT GGTCCTCATT
    TCCTCCGGGA AGTCGGATGA TGAGGTGGAG GACTCGGCCA TAGAGCTCAA GCAGTTTGGC
    GTGGCGCCAC TCACGATCGC CAGGAACGTG GACCAGGAAG AGCTGGTGAA GATCTCGCTG
    AGCCCCGAGT ACGTGTTCTC AGTGAACACC TTCCGGGAGC TCCCCAGCCT GGAGCAGAAA
    CTGCTGACAC CGCTCACCAC CCTGACTGCC GGGCAGATAC AGCAGCTCCT GGCCAGCACG
    CGCTACCCGC CTCCAGCTGT GGAGAGCGAT GCTGCGGACA TTGTCTTCCT CATCGACAGC
    TCTGATGGCG TGAAGCCGGA TGGCATCGCA CACATCCGGG ACTTTGTTAT CAGGATCGTG
    CGGAGACTCA ACGTCGGTCC CAACAAAGTG AGAATTGGGG TCCTGCAGTT CAGCAACGAC
    GTCTTCCCAG AATTTCAACT AAAGACCTAT AAATCCCAGG CCAGCGTGCT GGATGCCATA
    CGTCGCCTGA GGTTCAAAGG GGGGTCTCCT CTCAACACTG GCAAGGCTCT GGAATTCGTG
    GCGAGGAATT ACTTTGTCAA GTCTGCCGGG AGCCGGATAG AAGATGGGGT GCCCCAACAC
    CTGGTTCTAT TTCTGGGTGG AAAATCCCAG GATGATATTT CCAGGTATTC CCAGGTGATA
    AAATCTGCAG GAATTGCGAG CTTAGGGGTT GGAGACCGAA ATATTGACAG AACAGAGCTG
    CAGACCATCA CCAGCGATCC CAGGCTTGTC TTCACGGTGC GGGAGTTCAG AGACCTCCCC
    AGCATAGAAG AAAGGATGGT GAATTCCTTT GGGTCCTCCG GGGTCACCCC TGCACCTCCG
    GGAGTGGACA CACCCTCTCC TTCAAGGCCA GAGAAGAAGA AAGCCGACAT TGTGTTCCTG
    CTGGATGGTT CCATCAATTT CCGGAGAGAC AGTTTCCAGG AAGTGCTCCG TTTTGTGTCA
    GAAATTGTGG ACACGGTTTA TGAAGGGGGC GACTCCATCC AGGTGGGGCT GGTCCAATAC
    AACTCTGACC CCACCGACGA GTTCTTCCTG AAGGACTTCC CCACCAAGCA GCAGATTATC
    GACGCCATAA ACAAGGTGGT CTACAAAGGG GGGAGGCACG CGAACACCAA GGTGGGCCTG
    GAGCACCTGC GGCGGAACCA CTTCGTGCCC GAGGCGGGCA GCCGCCTGGA CCAGAGGGTC
    CCGCAGATCG CCTTTGTGAT CACGGGGGGC AAGTCGGTGG AGGACGCTCA GGAGGCAAGC
    ATGGCGCTCA CGCAGAGAGG CGTCAAGGTG TTTGCGGTGG GCGTAAGGAA CATCGACTCG
    GAGGAGGTTG GGAAGATAGC GTCCAACAGC GCCACGGCCT TCCGGGTGGG GAACGTCCAG
    GAGCTGTCGG AACTGAGCGA GCAGGTTTTG GAAACTCTGC ACGATGCAAT GCATGAGACC
    TTATGTCCAG GCGTGACTGA CGTTTCCAAA GCCTGTAACT TGGATGTGAT TCTGGGCTTC
    GATGGTTCAG GAGATCAGAA TGTCTTTGTG GCTCAGAAGG GCCTCGAGCC CAAGGTGGAC
    ACCATCCTGA GGAGAATCAG TCAGATGCAG AAGATCAGCT GCAGCGGCAG CCAGCTGCCC
    ACCGTGCGGG TGTCTGTGGT GGCCCTCACG CCCTCGGGCC CCGTTGAGGC CTTTGACTTT
    GCTGAGTACC AGCCGGAGCT GTTTGAGAAG TTCCAGAACA TGCGTGCCCA GCACCCCTAC
    GTCCTCACTG CGGACACACT GAAGCTCTAC CAGAACAAGT TCCGGCAGGC CTCGATGGAC
    AACGTGAAGG TGGTCATTCA TTTTACCGAT GGAGTGGATG GGGATCTGGC TGATTTACAA
    AGAGCATCAG AGGAACTCCG ACAAGAAGGT GTCCGAGCTC TGATCTTGGT GGGCCTCGAA
    CGCGTCGCCA ACTTGGAGCA GCTCATGCAG CTGGAGTTCG GGCGAGGGTT CACGTACAAC
    CGGCCCCTGA GGCTGAACTT GCTGGACCTG GACTACGAAC TAGCCGAGCA GCTTGACAAC
    ATTGCTGAGA AAGCCTGCTG TGGGATTCCC TGCAAATGCT CTGGACAAAG AGGAGACCGA
    GGGCCCATAG GCAGCATCGG GCCAAAGGGC ATTCCTGGAG AAGATGGCTA CCGAGGTTAT
    CCTGGTGACG AAGGTGGACC TGGTGAACGC GGTCCTCCTG GCGTGAACGG CACTCAAGGT
    TTCCAGGGCT GCCCCGGGCA GAGAGGAGTC AAGGGCTCTC GTGGATTCCC GGGAGAGAAG
    GGTGAATTAG GAGAAATTGG ACTGGATGGT CTTGATGGTG AAGATGGCGA CAAAGGGCTG
    CCCGGTACTT CTGGAGAGAA AGGGAGTCCA GGAAGGAGGG GCGACAAAGG ACCCAAAGGC
    GACAAAGGGG AGAGAGGCGA TGTCGGGATC AGAGGCGACC CGGGCAATTC AGGACAGGAC
    AGCCAGCAGA GAGGAGCCAA AGGAGAAACC GGAGACATCG GCCCCGTGGG TCTCCCTGGG
    AGAGACGGGG TGTCTGGAGG GCCTGGAGAA CCCGGGAAGA GCGGTGGCTT TGGCCGAAGG
    GGACCAGCGG GAGCTAAGGG CAACCAGGGC GGCCCCGGCC AGCAGGGCCC TGTAGGAGAG
    CAGGGGACCA GAGGCGCACA GGGTCCGCCT GGTCCCACCG GTCCTCCAGG CCTGATTGGT
    GAACAGGGCA TTCCCGGACC TCGGGGAGGC GGGGGAACCG CAGGTGCTCC TGGAGAACGC
    GGCAGAACCG GGCCCCTGGG AAGAAAGGGT GAGCCCGGAG ATCCAGGACC TAAGGGGAGC
    ATCGGGAACC GGGGTCCTCG TGGGGAGACG GGAGATGATG GGCGAGATGG AGTTGGCAGC
    GAAGGTCGCA TAGGCAAGAA GGGAGAAAGA GGATTCCCTG GATACCCAGG TCCAAAGGGC
    AATCCTGGTG AGCCAGGGAC AGACGGACCA CCGGGACCCA AAGGCATCAG AGGCCGAAGG
    GGAAACTCGG GACCTCCAGG GATAGCTGGA CAGAAGGGAG ACCCCGGTTA CCCAGGGCCA
    TCTGGTTACA AAGGCAGCAG AGGCGACTCA ATGGACCAAT GTGCGCTGGT CCAGAGCATC
    AAAGATAAAT GCCCTTGCTG TTACGGGCCC CTGGAGTGCC CCGTCTTCCC GACGGAGCTG
    GCTTTCGCCT TGGACACCTC AGAGGGGGTC ACCCAGGACA GGTTCAGCCA GATGAGGGAG
    GTGGTCCTCA AGATTCTGGA CGACCTGACC ATCGCCGAGA GCAACTGCCC GAGGGGGGCC
    CGCGTGGCCG TGGTCACTTA CAACAACGAG GTTACCACGG AGATCCGCTT TGCCGACTCC
    AAGAAGAAGT CCGTCCTCCT GGACAAGATC AAGAACCTGC AGGTGTCGCT GACCTCCAAG
    CAGCAGAGCC TGGAAACCGC CATGTCCTTT GTGGCCAGAA ACACGTTTAA GCGTGTGCGG
    AGCGGGTTCC TGATGAGGAA GGTGGCGGTG TTCTTCAGCA ACAAGCCCAC GAGGGCGACC
    CCGCAGCTCC GGGAGGCTGT GCTCAAGCTC TCAGATGCTG GGATCACACC CTTGTTCCTC
    ACAAGCCAGG AGGACCGGCA GCTGATCAAT GCCCTACAGA TCAATAACAC AGCGGTGGGA
    CATGCCCTTG TCCTTCCTGC TGGCGGAGAC CTCACAGACT TCTTGAAGAA TGTCCTGACG
    TGTCACGTCT GCTTGGACAT CTGCAACATT GACCCATCCT GTGGATTCGG CAGCTGGAGG
    CCTTCCTTCA GGGACCGGAG GGCAGCAGGC AGTGATGTGG ACATCGACAT GGCTTTCATC
    TTAGATAGCT CCGAGTCCAC CACCCCGTTC CAGTTCAACG AGATGAGGAA GTACATCGAG
    TACCTGGTCC GACAGCTGGA TGTGAGCCCA GACCCCAAGG CCTCCCAGCA CTTTGCCCGC
    GTGGCGGTGG TGCAGCATGC GCCCTACGAG GCGGTGGACA ACGCCAGTGT GCCGCCTGTG
    AAGGTGGAGT TCTCCCTGAC CGACTACGGC TCCAAAGAGA AGCTGCTGGC CTTCCTTGGC
    AGCAGGATGA CCCAGCTGCA GGGGACCAGG GCTTTGGGCC GCGCCATCGA CTACACCATT
    GAGAACATCT TCGAAAGTGC TCCCAACCCC CGGGACCTGA AGATCGTGGT GCTGATGCTG
    ACGGGCGAGG TGCAGAAGCA GCAGCTGGAG GAGGCCCAGA GAGCCATCCT ACAGGCCAAG
    TGCAAAGGCT ACTTCTTCGT GATCCTGGGC ATTGGCAGGA AGGTGAATGT CAAGGAGCTG
    TACAGCTCTG CCAGCGAGCC CAACGACGTC TTCTTCAAGC TAGTGGACAA GTCGACCGAG
    CTCAACGAGG AGCCCCTAAT GCGCTTCGGG AGGCTGCTGC CATCCTTCGT CAGCAGTAAA
    GATGCTTTCT ACCTGTCCCC AGATATCAGG AAACAGTGTG ATTGGTTCCA AGCAGACCAA
    CCGGCAAAGA ATCTCGTGCA GTTTGGTTAC AAACAAATAA ATGTTCCTAA TAACGTTACT
    TCAAGTCCTA CATCTAAACT GGTGACCACA ACGAAGCCGG TGATCACCAC CACCAAACCG
    ATCACAGTGG TGAACCTGCC AGCCTCAAAG CCAGCTGCCA CGAGACCTGT GGATGAGAGA
    CCCGCAGCTG GGAGATCCGT GGCCACCAAG ATGGACAGTG CCAGGCCCGT GGCCACCAAG
    ACCGAGGCCA CCAAACCCGT GGCCACCAAG ACCGAGGCCA CCAAGACTGA AGCTGCCAAA
    CCTGTGGTCA CCAAGGCTGA GGCTGCAAAA CCCACGGCCA CCAAGACTGA AGCCACTAAG
    ACCGAGGCTG CAAAGCTCAT GGCCACCAAG ACCGAGGCCA CCAAACCTGT GGCTGCCAGG
    CCCGAGGCGG CAAAGACAGC CACAGCCAGA CCTGCAGCAG CCGCGAAGCC TGCAGCAGCC
    AAACCTGCCC CTGCCAGACC CCCCACTGCT GCCAGATCCA TGGCCACGAG GTCCGAGGCC
    CCCAGGCCCC AGGCAGTCAA ACTGGCTGCC AGCAGGCCCG GCGCTGCTAA GCCCGTGGTG
    AAGGCACCTC GCGAGATCCA TGTGTCTGAG GTCACAGAGA ACAGCGCCAA GCTGCACTGG
    GAGAGGCCCG AGCCCCCCAG CCCTTACTTA TACAACCTCA CCGTCACCTC GGCCCACGAT
    CAGGCCGTGG TTCTGAAGCA GAACCTCACT GTCACGGACC GCGTCATCGG GGGCCTGCTC
    CCCGGGCAGA CTTACCACGT TACGGTGACC TGCTCCCTGA GGTCTCAGGT CAGAGCCATC
    TACCAAGGCA GCTTCAGCAC GAAGAAAATT CAGCCTCCAC CTCCACAAAC AGAGCGATCA
    GCCTCTAGTG CAACCATCAA CCTAGTGGTG AGCGCAGACC GGTTGGCTGG GAGTAAAGCA
    GATATATGTA AGTTGCCAAA GGACGGCGGA ACTTGCAGAG AGTTTGTATT AAAGTGGTAC
    TATGATTCCG TAACTGAAAA CTGCGCCCGA TTCTGGTATG GAGGCTGCGG TGGAAACGAA
    AACAGATTTA ATTCGCAGGA TGAATGTGAA AAGGTTTGCC CTCCTGTGCC CATCAAGCAG
    CCCGGAGTTA TCGCCGCGAT GGGGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.