EGF cDNA ORF clone, Bos taurus(cattle)

The following EGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235743 XM_024993778.1
Latest version!
Bos taurus epidermal growth factor (EGF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa90070 XM_015471508.1
Latest version!
Bos taurus epidermal growth factor (EGF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa90070 XM_015463717.1
Latest version!
Bos taurus epidermal growth factor (EGF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235743
    Clone ID Related Accession (Same CDS sequence) XM_024993778.1
    Accession Version XM_024993778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: pro-epidermal growth factor
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases frameshifts :: corrected 5 indels internal stop codons :: corrected 3 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCATT TCCTTGTCAT TGTGTTGCTG GTAGTTTTCA AATTTAGTTT TGTTAGCCTC 
    TCAGCCCTGC CGCACTTGAA CTGTCCTGAA GGCTCTCCCT CTGGAAATGG GAATTCTACT
    TGTGCAGGTC TTGTGCCCTT TTTAATTTTT TCCCATGGAA ATAGTATCTG TAGGATTGAT
    TTGGAAGGAA TGAATTATGA ACAATTGGTG GCAGATGCTG GCATCTCAGT GATCATGGAT
    TTTCATTACA ATGAAGAAAG AATCTATTGG GTTGATCTAC AAAGACAACT CTTGCAAAGA
    GTTCATTTGA ATGGCAGAAG GCAAGAGAAA GTATATGATA TAGATAAAAA TGTTTCAGGA
    ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCTA ATCAACAGGA AGGAATCATT
    ACAGTAACTG ATAGGAAAGG AAACAATTCC TGGGTTCTCT TAAGTGCCTT AAAATATCCT
    GCAAATATAG CAGTTGATCC AGTAGCAAGT TTTATGTTTT GGTCTTCAGA GGTGGCTNGC
    AGCCTTCACA GAGCAGATCT TGGTGGTATG GAAGTCAAGC TTCTGTTAGA GACATCAGAG
    AAAATAGCAG CTGTATCATT GGATATGCTT GATAAACGGC TCTTTTGGAT TCAGTACCAT
    AGAGGAGGAG GTGATCCTCG TATTTGTTCC TGTGATTATG ACGGGGGTTC TGTCCTTTTT
    AGCAAATATT CCCTGAGGCA CAATCTGCTT GCAGAGTCCT TCTTTGGAGA TTGTATCTTC
    TATTCAACAT AGAAAAAGAA GACAATTTGG ATAGCTAACA AGCACACAGG GAAGGATATG
    GTTAAAATCA CCCTCAACTC GTCATTCGTG CCACCCAGTG GAATTAAAGT GGTTCGTCCA
    CTCGTATAGC CCAAGGCAGA GGGTGATGCT TGGGCATCTG ATGTCAGTGA GTGTGCTTTT
    CGGAATCATG GCTACACAGA TGACTGTGAA AATATCCCTG GATCCTATTA CTGCACATGC
    CCTGAGGGAT ATGTCCTGCT TCCTGATGGG AAATGGTGTC ACCAGTTAAT TTCCTGCCCA
    AGAAATGTGT CTAAATGCAG TCATGACTGT GTTCTGACAT CAGACCGTCC CATATGTTCC
    TGTCCTGAAG GCTCAGTGCT TGACACAGAT GGAAAGACAT GCAGTGGCTG TTCATCACCT
    GATAATGGGG GATGTAGCCA GCCCTGCACC CCTCTCAGCC CCGTATCCTG GAAATGTGGC
    TGCCTTCCTG GGTATGACCT ACAACTGGAC AAAAAGAGCC ATAGACCTTC AGGACCACCA
    CCATTTTTGC TGTTTGCCAG TTCTCAAGAC ATTCGATACA TGCATTTTGA TGGAACAGAT
    TATGGAACCC TGGTCTACCA ACAAATAATA GGAATGGTTT TTGCCCTTGA TCATGACCCT
    GTGGAAAATA AGACTGTATG CCTGTGTTCT CTTTTGTTGA TATACTTTGC CCATACAGTC
    CTGAAGTGGA TAGAAAGAGC TAATATGGAT GCTTCCCAGC GANGAAGGCT TATCGAGGAA
    GAAGTAGGTT TACCAGAAGG TCTTGCTGTT GACTGGATTG GCCATAAACT CTACTGGACA
    GACAGAGGGA AATCTCTCAT TGAAGGGAGT GATTTAAATG GAAAGTATTG TGAAATAATC
    ATTAAGGAGG GCATCTCTCA GCCACGAGGA NATGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATATAGGAAT TAATCCACGA ATTGAAAGTT CTTCTCTTCA AGGCAGTGGC
    CGACTGGTTA TAGCTAGCTC GGATCTAGTC TGGCACAGTG GAATAGCAGT TGATTACTTA
    ACTGACAAAT TGTATTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGTC CAATCTGGAT
    GGCTCAAAAT GCCAAAGACT TCCCCAGAAG GATGTAGGTC ACCCATTTGC CGTGGCTGTG
    TTTGAGGATC ACGTGTGGTT CTCTGATTGG ACTATGCCAT CGATAACAAG GATGAACAAG
    AGGACTGGCA AAAATAGCGT ACGTCTCCCA GGCAGCATGC TGAAGCCCTC ATCTCTGCTT
    GTAGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TACATCAAAA TGGAGGCTGT
    GAACATATTT GCAAAGAGAG GTTTGAAACT GCTCAGTGTT TGTGTCATGA AGGTTTTGTG
    AAAGCTCCAG ATGGAAAAAT GTGTCTGGCT CTTCATGGCC ATCGGATATT GTCAGATAAT
    ATGACAAACT GTAGTGGAGC CAATCTGAGT AGTGAAGTCC TGCCACTGGA CATCTTAGCC
    GGCAGCAGAG AGCTTGACTA TAACATGGCA GAGTCTCAAC ACCTGCCAGT GGCCGAGATC
    ATGGTGTCAG ATTTGTTGAG AGCATGTAAA CAGAAGCATA TGGCTTGTGG ACCCTTTGAT
    ATAGATACAG ATTACAGATC AACATGGTGT TTCTGTTTGG AGGTTAACCT GTCCCAGAAC
    TTGACAAAAG AAGTTCTAGA ATGTTCTATA ATAAAGACTG ATGCCATGGG ATGCAGTACA
    CGGGCTGAGT GCCTTTCAAA GGGAGCAGAT GTCACATGTC AGTGTTTGAA AGGATTTGCT
    GGAGATGGAA AACAATGTTC TGATGTAGAT GGATGTGAGA TGGGTCTCTC AGTTTGCCCT
    CCCACCTCCC CCAAGTGTGT CAATACAGAA GGTGGCTATG TCTGCTGGCG CTCAGAGAGC
    TACCAGGGAG ATGGAATCCA CTGTTTGGAT ATTGATGAGT GCCGACGGGG CGTGCACAGC
    TGTGGGGAAA ATGCCACCTG AAAAAATATG GAGGGAAACC ACACTTGCAC GTGTGCTGGC
    GACTTGTCTG AGCCTGGACA GATTTGCCCT GACTCTACTC TGCTGTCTCA CCTTGGGAAG
    AATGGACACA ATTTTTTGAA AAAATGTTTC CCTGAATATA CCCCGAATTT TGAAGGGTAC
    TGCCTCAATG GTCATGTCTG TATATATTTT GGCATTGCCA ACCTGTTCTC CTGCCACTGT
    CCCATTGGCT ACCCTGGGAA GCGAGGTGAG TACATAGACT TCGATGGGTG GGATCCGCAC
    AGTGCAGGCC GTGGGCATCA GTGGAACACC AGCCCGGTGG CTGTCCGTGC GCTGGTGCTG
    GCTTTCCTGC TGCTCCTCGG GCTGTGCAGA GCTCACTACT ACTACAGGAC TCAGAAGTGG
    CTACTGAAAA ATCCAAAGAA TCCTTGTGAA GAACCGAGCA GAGATGTGAT CCGTAGCAGG
    CCTGCAGATG GCGAGGCTGG ANTGCCTTTT TGCCCCCAAC CTTGGTTCGT GGTTGTAAAG
    GAACATCAAA ACCTCAGGAA TGGGCGTCCA TCCGAGGCCC TCAAGGAAGG CCTGGCAGCA
    GATGTTGGCC AGTTTTCCCC CCTGGATCCT GGTGAAGGGG TGAAGTGTAG CTTTCATCTC
    CTCTCCGAAG GGGCATGGCC CATTGCTGAG GGGCTTGGGA CATCCTGTCC TCTCCCATCA
    GCTCACCCTT TGTGGCAACA AAGATCTCCA CACCAAATGG AGCTGACTCT GAAAACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849546.1
    CDS1..3600
    Translation

    Target ORF information:

    RefSeq Version XM_024993778.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epidermal growth factor (EGF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCATT TCCTTGTCAT TGTGTTGCTG GTAGTTTTCA AATTTAGTTT TGTTAGCCTC 
    TCAGCCCTGC CGCACTTGAA CTGTCCTGAA GGCTCTCCCT CTGGAAATGG GAATTCTACT
    TGTGCAGGTC TTGTGCCCTT TTTAATTTTT TCCCATGGAA ATAGTATCTG TAGGATTGAT
    TTGGAAGGAA TGAATTATGA ACAATTGGTG GCAGATGCTG GCATCTCAGT GATCATGGAT
    TTTCATTACA ATGAAGAAAG AATCTATTGG GTTGATCTAC AAAGACAACT CTTGCAAAGA
    GTTCATTTGA ATGGCAGAAG GCAAGAGAAA GTATATGATA TAGATAAAAA TGTTTCAGGA
    ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCTA ATCAACAGGA AGGAATCATT
    ACAGTAACTG ATAGGAAAGG AAACAATTCC TGGGTTCTCT TAAGTGCCTT AAAATATCCT
    GCAAATATAG CAGTTGATCC AGTAGCAAGT TTTATGTTTT GGTCTTCAGA GGTGGCTNGC
    AGCCTTCACA GAGCAGATCT TGGTGGTATG GAAGTCAAGC TTCTGTTAGA GACATCAGAG
    AAAATAGCAG CTGTATCATT GGATATGCTT GATAAACGGC TCTTTTGGAT TCAGTACCAT
    AGAGGAGGAG GTGATCCTCG TATTTGTTCC TGTGATTATG ACGGGGGTTC TGTCCTTTTT
    AGCAAATATT CCCTGAGGCA CAATCTGCTT GCAGAGTCCT TCTTTGGAGA TTGTATCTTC
    TATTCAACAT AGAAAAAGAA GACAATTTGG ATAGCTAACA AGCACACAGG GAAGGATATG
    GTTAAAATCA CCCTCAACTC GTCATTCGTG CCACCCAGTG GAATTAAAGT GGTTCGTCCA
    CTCGTATAGC CCAAGGCAGA GGGTGATGCT TGGGCATCTG ATGTCAGTGA GTGTGCTTTT
    CGGAATCATG GCTACACAGA TGACTGTGAA AATATCCCTG GATCCTATTA CTGCACATGC
    CCTGAGGGAT ATGTCCTGCT TCCTGATGGG AAATGGTGTC ACCAGTTAAT TTCCTGCCCA
    AGAAATGTGT CTAAATGCAG TCATGACTGT GTTCTGACAT CAGACCGTCC CATATGTTCC
    TGTCCTGAAG GCTCAGTGCT TGACACAGAT GGAAAGACAT GCAGTGGCTG TTCATCACCT
    GATAATGGGG GATGTAGCCA GCCCTGCACC CCTCTCAGCC CCGTATCCTG GAAATGTGGC
    TGCCTTCCTG GGTATGACCT ACAACTGGAC AAAAAGAGCC ATAGACCTTC AGGACCACCA
    CCATTTTTGC TGTTTGCCAG TTCTCAAGAC ATTCGATACA TGCATTTTGA TGGAACAGAT
    TATGGAACCC TGGTCTACCA ACAAATAATA GGAATGGTTT TTGCCCTTGA TCATGACCCT
    GTGGAAAATA AGACTGTATG CCTGTGTTCT CTTTTGTTGA TATACTTTGC CCATACAGTC
    CTGAAGTGGA TAGAAAGAGC TAATATGGAT GCTTCCCAGC GANGAAGGCT TATCGAGGAA
    GAAGTAGGTT TACCAGAAGG TCTTGCTGTT GACTGGATTG GCCATAAACT CTACTGGACA
    GACAGAGGGA AATCTCTCAT TGAAGGGAGT GATTTAAATG GAAAGTATTG TGAAATAATC
    ATTAAGGAGG GCATCTCTCA GCCACGAGGA NATGCTGTTC ATCCAATGGC CAAGAGATTA
    TTCTGGACTG ATATAGGAAT TAATCCACGA ATTGAAAGTT CTTCTCTTCA AGGCAGTGGC
    CGACTGGTTA TAGCTAGCTC GGATCTAGTC TGGCACAGTG GAATAGCAGT TGATTACTTA
    ACTGACAAAT TGTATTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGTC CAATCTGGAT
    GGCTCAAAAT GCCAAAGACT TCCCCAGAAG GATGTAGGTC ACCCATTTGC CGTGGCTGTG
    TTTGAGGATC ACGTGTGGTT CTCTGATTGG ACTATGCCAT CGATAACAAG GATGAACAAG
    AGGACTGGCA AAAATAGCGT ACGTCTCCCA GGCAGCATGC TGAAGCCCTC ATCTCTGCTT
    GTAGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TACATCAAAA TGGAGGCTGT
    GAACATATTT GCAAAGAGAG GTTTGAAACT GCTCAGTGTT TGTGTCATGA AGGTTTTGTG
    AAAGCTCCAG ATGGAAAAAT GTGTCTGGCT CTTCATGGCC ATCGGATATT GTCAGATAAT
    ATGACAAACT GTAGTGGAGC CAATCTGAGT AGTGAAGTCC TGCCACTGGA CATCTTAGCC
    GGCAGCAGAG AGCTTGACTA TAACATGGCA GAGTCTCAAC ACCTGCCAGT GGCCGAGATC
    ATGGTGTCAG ATTTGTTGAG AGCATGTAAA CAGAAGCATA TGGCTTGTGG ACCCTTTGAT
    ATAGATACAG ATTACAGATC AACATGGTGT TTCTGTTTGG AGGTTAACCT GTCCCAGAAC
    TTGACAAAAG AAGTTCTAGA ATGTTCTATA ATAAAGACTG ATGCCATGGG ATGCAGTACA
    CGGGCTGAGT GCCTTTCAAA GGGAGCAGAT GTCACATGTC AGTGTTTGAA AGGATTTGCT
    GGAGATGGAA AACAATGTTC TGATGTAGAT GGATGTGAGA TGGGTCTCTC AGTTTGCCCT
    CCCACCTCCC CCAAGTGTGT CAATACAGAA GGTGGCTATG TCTGCTGGCG CTCAGAGAGC
    TACCAGGGAG ATGGAATCCA CTGTTTGGAT ATTGATGAGT GCCGACGGGG CGTGCACAGC
    TGTGGGGAAA ATGCCACCTG AAAAAATATG GAGGGAAACC ACACTTGCAC GTGTGCTGGC
    GACTTGTCTG AGCCTGGACA GATTTGCCCT GACTCTACTC TGCTGTCTCA CCTTGGGAAG
    AATGGACACA ATTTTTTGAA AAAATGTTTC CCTGAATATA CCCCGAATTT TGAAGGGTAC
    TGCCTCAATG GTCATGTCTG TATATATTTT GGCATTGCCA ACCTGTTCTC CTGCCACTGT
    CCCATTGGCT ACCCTGGGAA GCGAGGTGAG TACATAGACT TCGATGGGTG GGATCCGCAC
    AGTGCAGGCC GTGGGCATCA GTGGAACACC AGCCCGGTGG CTGTCCGTGC GCTGGTGCTG
    GCTTTCCTGC TGCTCCTCGG GCTGTGCAGA GCTCACTACT ACTACAGGAC TCAGAAGTGG
    CTACTGAAAA ATCCAAAGAA TCCTTGTGAA GAACCGAGCA GAGATGTGAT CCGTAGCAGG
    CCTGCAGATG GCGAGGCTGG ANTGCCTTTT TGCCCCCAAC CTTGGTTCGT GGTTGTAAAG
    GAACATCAAA ACCTCAGGAA TGGGCGTCCA TCCGAGGCCC TCAAGGAAGG CCTGGCAGCA
    GATGTTGGCC AGTTTTCCCC CCTGGATCCT GGTGAAGGGG TGAAGTGTAG CTTTCATCTC
    CTCTCCGAAG GGGCATGGCC CATTGCTGAG GGGCTTGGGA CATCCTGTCC TCTCCCATCA
    GCTCACCCTT TGTGGCAACA AAGATCTCCA CACCAAATGG AGCTGACTCT GAAAACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90070
    Clone ID Related Accession (Same CDS sequence) XM_015463717.1 , XM_015471508.1
    Accession Version XM_015463717.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: pro-epidermal growth factor
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases frameshifts :: corrected 6 indels internal stop codons :: corrected 3 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCATT TCCTTGTCAT TGTGTTGCTG GTAGTTTTCA AATTTAGTTT TGTTAGCCTC 
    TCAGCCCTGC CGCACTTGAA CTGTCCTGAA GGCTCTCCCT CTGGAAATGG GAATTCTACT
    TGTGCAGGTC TTGTGCCCTT TTTAATTTTT TCCCATGGAA ATAGTATCTG TAGGATTGAT
    TTGGAAGGAA TGAATTATGA ACAATTGGTG GCAGATGCTG GCATCTCAGT GATCATGGAT
    TTTCATTACA ATGAAGAAAG AATCTATTGG GTTGATCTAN NCAGACAACT CTTGCAAAGA
    GTTCATTTGA ATGGGAGAAG GCAAGAGAAA GTATATGATA TAGATAAAAA TGTTTCAGGA
    ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCTA ATCAACAGGA AGGAATCATT
    ACAGTAACTG ATAGGAAAGG AAACAATTCC TGGGTTCTCT TAAGTGCCTT AAAATATCCT
    GCAAATATAG CAGTTGATCC AGTAGCAAGT TTTATGTTTT GGTCTTCAGA GGTGGCTNGC
    AGCCTTCACA GAGCAGATCT TGGTGGTATG GAAGTCAAGC TTCTGTTAGA GACATCAGAG
    AAAATAGCAG CTGTATCATT GGATATGCTT GATAAACGGC TCTTTTGGAT TCAGTACCAT
    AGAGGAGGAG GTGATCCTCG TATTTGTTCC TGTGATTATG ACGGGGGTTC TGTCCTTTTT
    AGCAAATATT CCCTGAGGCA CAATCTGCTT GCAGAGTCCT TCTTTGGAGA TTGTATCTTC
    TATTCAACAT AGAAAAAGAA GACAATTTGG ATAGCTAACA AGCACACAGG GAAGGATATG
    GTTAAAATCA CCCTCAACTC GTCATTCGTG CCACCCAGTG GAATTAAAGT GGTTCGTCCA
    CTCGTATAGC CCAAGGCAGA GGGTGATGCT TGGGCATCTG ATGTCAGTGA GTGTGCTTTT
    CGGAATCATG GCTACACAGA TGACTGTGAA AATATCCCTG GATCCTATTA CTGCACATGC
    CCTGAGGGAT ATGTCCTGCT TCCTGATGGG AAATGGTGTC ACCAGTTAAT TTCCTGCCCA
    AGAAATGTGT CTAAATGCAG TCATGACTGT GTTCTGACAT CAGACCGTCC CATATGTTCC
    TGTCCTGAAG GCTCAGTGCT TGACACAGAT GGAAAGACAT GCAGTGGCTG TTCATCACCT
    GATAATGGGG GATGTAGCCA GCCCTGCACC CCTCTCAGCC CCGTATCCTG GAAATGTGGC
    TGCCTTCCTG GGTATGACCT ACAACTGGAC AAAAAGAGCC ATAGACCTTC AGGACCACCA
    CCATTTTTGC TGTTTGCCAG TTCTCAAGAC ATTCGATACA TGCATTTTGA TGGAACAGAT
    TATGGAACCC TGGTCTACCA ACAAATAATA GGAATGGTTT TTGCCCTTGA TCATGACCCT
    GTGGAAAATA AGATATACTT TGCCCATACA GTCCTGAAGT GGATAGAAAG AGCTAATATG
    GATGCTTCCC AGCGANGAAG GCTTATCGAG GAAGAAGTAG GTTTACCAGA AGGTCTTGCT
    GTTGACTGGA TTGGCCATAA ACTCTACTGG ACAGACAGAG GGAAATCTCT CATTGAAGGG
    AGTGATTTAA ATGGAAAGTA TTGTGAAATA ATCATTAAGG AGGGCATCTC TCAGCCACGA
    GGANATGCTG TTCATCCAAT GGCCAAGAGA TTATTCTGGA CTGATATAGG AATTAATCCA
    CGAATTGAAA GTTCTTCTCT TCAAGGCAGT GGCCGACTGG TTATAGCTAG CTCGGATCTA
    GTCTGGCACA GTGGAATAGC AGTTGATTAC TTAACTGACA AGTTGTATTG GTGCGATGCC
    AAGCAGTCTG TGATTGAAAT GTCCAATCTG GATGGCTCAA AATGCCAAAG ACTTCCCCAG
    AAGGATGTAG GTCACCCATT TGCCGTGGCT GTGTTTGAGG ATCACGTGTG GTTCTCTGAT
    TGGACTATGC CATCGATAAC AAGGATGAAC AAGAGGACTG GCAAAAATAG CGTACGTCTC
    CCAGGCAGCA TGCTGAAGCC CTCATCTCTG CTTGTAGTTC ATCCATTGGC AAAACCAGGA
    GCAGATCCCT GCTTACATCA AAATGGAGGC TGTGAACATA TTTGCAAAGA GAGGTTTGAA
    ACTGCTCAGT GTTTGTGTCA TGAAGGTTTT GTGAAAGCTC CAGATGGAAA AATGTGTCTG
    GCTCTTCATG GCCATCGGAT ATTGTCAGAT AATATGACAA ACTGTAGTGG AGCCAATCTG
    AGTAGTGAAG TCCTGCCACT GGACATCTTA GCCGGCAGCA GAGAGCTTGA CTATAACATG
    GCAGAGTCTC AACACCTGCC AGTGGCCGAG ATCATGGTGT CAGATTTGTT GAGAGCATGT
    AAACAGAAGC ATATGGCTTG TGGACCCTTT GATATAGATA CAGATTACAG ATCAACATGG
    TGTTTCTGTT TGGAGGTTAA CCTGTCCCAG AACTTGACAA AAGAAGTTCT AGAATGTTCT
    ATAATAAAGA CTGATGCCAT GGGATGCAGT ACACGGGCTG AGTGCCTTTC AAAGGGAGCA
    GATGTCACAT GTCAGTGTTT GAAAGGATTT GCTGGAGATG GAAAACAATG TTCTGATGTA
    GATGGATGTG AGATGGGTCT CTCAGTTTGC CCTCCCACCT CCCCCAAGTG TGTCAATACA
    GAAGGTGGCT ATGTCTGCTG GCGCTCAGAG AGCTACCAGG GAGATGGAAT CCACTGTTTG
    GATATTGATG AGTGCCGACG GGGCGTGCAC AGCTGTGGGG AAAATGCCAC CTGAAAAAAT
    ATGGAGGGAA ACCACACTTG CACGTGTGCT GGCGACTTGT CTGAGCCTGG ACAGATTTGC
    CCTGACTCTA CTCTGCTGTC TCACCTTGGG AAGAATGGAC ACAATTTTTT GAAAAAATGT
    TTCCCTGAAT ATACCCCGAA TTTTGAAGGG TACTGCCTCA ATGGTCATGT CTGTATATAT
    TTTGGCATTG CCAACCTGTT CTCCTGCCAC TGTCCCATTG GCTACCCTGG GAAGCGAGGT
    GAGTACATAG ACTTCGATGG GTGGGATCCG CACAGTGCAG GCCGTGGGCA TCAGTGGAAC
    ACCAGCCCGG TGGCTGTCCG TGCGCTGGTG CTGGCTTTCC TGCTGCTCCT CGGGCTGTGC
    AGAGCTCACT ACTACTACAG GACTCAGAAG TGGCTACTGA AAAATCCAAA GAATCCTTGT
    GAAGAACCGA GCAGAGATGT GATCCGTAGC AGGCCTGCAG ATGGCGAGGC TGGANTGCCT
    TTTTGCCCCC AACCTTGGTT CGTGGTTGTA AAGGAACATC AAAACCTCAG GAATGGGCGT
    CCATCCGAGG CCCTCAAGGA AGGCCTGGCA GCAGATGTTG GCCAGTTTTC CCCCCTGGAT
    CCTGGACCAG GGGTGAAGTG TAGCTTTCAT CTCCTCTCCG AAGGGGCATG GCCCATTGCT
    GAGGGGCTTG GGACATCCTG TCCTCTCCCA TCAGCTCACC CTTTGTGGCA ACAAAGATCT
    CCACACCAAA TGGAGCTGAC TCTGAAAACT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319203.1
    CDS1..3573
    Translation

    Target ORF information:

    RefSeq Version XM_015463717.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epidermal growth factor (EGF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCATT TCCTTGTCAT TGTGTTGCTG GTAGTTTTCA AATTTAGTTT TGTTAGCCTC 
    TCAGCCCTGC CGCACTTGAA CTGTCCTGAA GGCTCTCCCT CTGGAAATGG GAATTCTACT
    TGTGCAGGTC TTGTGCCCTT TTTAATTTTT TCCCATGGAA ATAGTATCTG TAGGATTGAT
    TTGGAAGGAA TGAATTATGA ACAATTGGTG GCAGATGCTG GCATCTCAGT GATCATGGAT
    TTTCATTACA ATGAAGAAAG AATCTATTGG GTTGATCTAN NCAGACAACT CTTGCAAAGA
    GTTCATTTGA ATGGGAGAAG GCAAGAGAAA GTATATGATA TAGATAAAAA TGTTTCAGGA
    ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCTA ATCAACAGGA AGGAATCATT
    ACAGTAACTG ATAGGAAAGG AAACAATTCC TGGGTTCTCT TAAGTGCCTT AAAATATCCT
    GCAAATATAG CAGTTGATCC AGTAGCAAGT TTTATGTTTT GGTCTTCAGA GGTGGCTNGC
    AGCCTTCACA GAGCAGATCT TGGTGGTATG GAAGTCAAGC TTCTGTTAGA GACATCAGAG
    AAAATAGCAG CTGTATCATT GGATATGCTT GATAAACGGC TCTTTTGGAT TCAGTACCAT
    AGAGGAGGAG GTGATCCTCG TATTTGTTCC TGTGATTATG ACGGGGGTTC TGTCCTTTTT
    AGCAAATATT CCCTGAGGCA CAATCTGCTT GCAGAGTCCT TCTTTGGAGA TTGTATCTTC
    TATTCAACAT AGAAAAAGAA GACAATTTGG ATAGCTAACA AGCACACAGG GAAGGATATG
    GTTAAAATCA CCCTCAACTC GTCATTCGTG CCACCCAGTG GAATTAAAGT GGTTCGTCCA
    CTCGTATAGC CCAAGGCAGA GGGTGATGCT TGGGCATCTG ATGTCAGTGA GTGTGCTTTT
    CGGAATCATG GCTACACAGA TGACTGTGAA AATATCCCTG GATCCTATTA CTGCACATGC
    CCTGAGGGAT ATGTCCTGCT TCCTGATGGG AAATGGTGTC ACCAGTTAAT TTCCTGCCCA
    AGAAATGTGT CTAAATGCAG TCATGACTGT GTTCTGACAT CAGACCGTCC CATATGTTCC
    TGTCCTGAAG GCTCAGTGCT TGACACAGAT GGAAAGACAT GCAGTGGCTG TTCATCACCT
    GATAATGGGG GATGTAGCCA GCCCTGCACC CCTCTCAGCC CCGTATCCTG GAAATGTGGC
    TGCCTTCCTG GGTATGACCT ACAACTGGAC AAAAAGAGCC ATAGACCTTC AGGACCACCA
    CCATTTTTGC TGTTTGCCAG TTCTCAAGAC ATTCGATACA TGCATTTTGA TGGAACAGAT
    TATGGAACCC TGGTCTACCA ACAAATAATA GGAATGGTTT TTGCCCTTGA TCATGACCCT
    GTGGAAAATA AGATATACTT TGCCCATACA GTCCTGAAGT GGATAGAAAG AGCTAATATG
    GATGCTTCCC AGCGANGAAG GCTTATCGAG GAAGAAGTAG GTTTACCAGA AGGTCTTGCT
    GTTGACTGGA TTGGCCATAA ACTCTACTGG ACAGACAGAG GGAAATCTCT CATTGAAGGG
    AGTGATTTAA ATGGAAAGTA TTGTGAAATA ATCATTAAGG AGGGCATCTC TCAGCCACGA
    GGANATGCTG TTCATCCAAT GGCCAAGAGA TTATTCTGGA CTGATATAGG AATTAATCCA
    CGAATTGAAA GTTCTTCTCT TCAAGGCAGT GGCCGACTGG TTATAGCTAG CTCGGATCTA
    GTCTGGCACA GTGGAATAGC AGTTGATTAC TTAACTGACA AGTTGTATTG GTGCGATGCC
    AAGCAGTCTG TGATTGAAAT GTCCAATCTG GATGGCTCAA AATGCCAAAG ACTTCCCCAG
    AAGGATGTAG GTCACCCATT TGCCGTGGCT GTGTTTGAGG ATCACGTGTG GTTCTCTGAT
    TGGACTATGC CATCGATAAC AAGGATGAAC AAGAGGACTG GCAAAAATAG CGTACGTCTC
    CCAGGCAGCA TGCTGAAGCC CTCATCTCTG CTTGTAGTTC ATCCATTGGC AAAACCAGGA
    GCAGATCCCT GCTTACATCA AAATGGAGGC TGTGAACATA TTTGCAAAGA GAGGTTTGAA
    ACTGCTCAGT GTTTGTGTCA TGAAGGTTTT GTGAAAGCTC CAGATGGAAA AATGTGTCTG
    GCTCTTCATG GCCATCGGAT ATTGTCAGAT AATATGACAA ACTGTAGTGG AGCCAATCTG
    AGTAGTGAAG TCCTGCCACT GGACATCTTA GCCGGCAGCA GAGAGCTTGA CTATAACATG
    GCAGAGTCTC AACACCTGCC AGTGGCCGAG ATCATGGTGT CAGATTTGTT GAGAGCATGT
    AAACAGAAGC ATATGGCTTG TGGACCCTTT GATATAGATA CAGATTACAG ATCAACATGG
    TGTTTCTGTT TGGAGGTTAA CCTGTCCCAG AACTTGACAA AAGAAGTTCT AGAATGTTCT
    ATAATAAAGA CTGATGCCAT GGGATGCAGT ACACGGGCTG AGTGCCTTTC AAAGGGAGCA
    GATGTCACAT GTCAGTGTTT GAAAGGATTT GCTGGAGATG GAAAACAATG TTCTGATGTA
    GATGGATGTG AGATGGGTCT CTCAGTTTGC CCTCCCACCT CCCCCAAGTG TGTCAATACA
    GAAGGTGGCT ATGTCTGCTG GCGCTCAGAG AGCTACCAGG GAGATGGAAT CCACTGTTTG
    GATATTGATG AGTGCCGACG GGGCGTGCAC AGCTGTGGGG AAAATGCCAC CTGAAAAAAT
    ATGGAGGGAA ACCACACTTG CACGTGTGCT GGCGACTTGT CTGAGCCTGG ACAGATTTGC
    CCTGACTCTA CTCTGCTGTC TCACCTTGGG AAGAATGGAC ACAATTTTTT GAAAAAATGT
    TTCCCTGAAT ATACCCCGAA TTTTGAAGGG TACTGCCTCA ATGGTCATGT CTGTATATAT
    TTTGGCATTG CCAACCTGTT CTCCTGCCAC TGTCCCATTG GCTACCCTGG GAAGCGAGGT
    GAGTACATAG ACTTCGATGG GTGGGATCCG CACAGTGCAG GCCGTGGGCA TCAGTGGAAC
    ACCAGCCCGG TGGCTGTCCG TGCGCTGGTG CTGGCTTTCC TGCTGCTCCT CGGGCTGTGC
    AGAGCTCACT ACTACTACAG GACTCAGAAG TGGCTACTGA AAAATCCAAA GAATCCTTGT
    GAAGAACCGA GCAGAGATGT GATCCGTAGC AGGCCTGCAG ATGGCGAGGC TGGANTGCCT
    TTTTGCCCCC AACCTTGGTT CGTGGTTGTA AAGGAACATC AAAACCTCAG GAATGGGCGT
    CCATCCGAGG CCCTCAAGGA AGGCCTGGCA GCAGATGTTG GCCAGTTTTC CCCCCTGGAT
    CCTGGACCAG GGGTGAAGTG TAGCTTTCAT CTCCTCTCCG AAGGGGCATG GCCCATTGCT
    GAGGGGCTTG GGACATCCTG TCCTCTCCCA TCAGCTCACC CTTTGTGGCA ACAAAGATCT
    CCACACCAAA TGGAGCTGAC TCTGAAAACT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90070
    Clone ID Related Accession (Same CDS sequence) XM_015463717.1 , XM_015471508.1
    Accession Version XM_015471508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: pro-epidermal growth factor
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases frameshifts :: corrected 6 indels internal stop codons :: corrected 3 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCATT TCCTTGTCAT TGTGTTGCTG GTAGTTTTCA AATTTAGTTT TGTTAGCCTC 
    TCAGCCCTGC CGCACTTGAA CTGTCCTGAA GGCTCTCCCT CTGGAAATGG GAATTCTACT
    TGTGCAGGTC TTGTGCCCTT TTTAATTTTT TCCCATGGAA ATAGTATCTG TAGGATTGAT
    TTGGAAGGAA TGAATTATGA ACAATTGGTG GCAGATGCTG GCATCTCAGT GATCATGGAT
    TTTCATTACA ATGAAGAAAG AATCTATTGG GTTGATCTAN NCAGACAACT CTTGCAAAGA
    GTTCATTTGA ATGGGAGAAG GCAAGAGAAA GTATATGATA TAGATAAAAA TGTTTCAGGA
    ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCTA ATCAACAGGA AGGAATCATT
    ACAGTAACTG ATAGGAAAGG AAACAATTCC TGGGTTCTCT TAAGTGCCTT AAAATATCCT
    GCAAATATAG CAGTTGATCC AGTAGCAAGT TTTATGTTTT GGTCTTCAGA GGTGGCTNGC
    AGCCTTCACA GAGCAGATCT TGGTGGTATG GAAGTCAAGC TTCTGTTAGA GACATCAGAG
    AAAATAGCAG CTGTATCATT GGATATGCTT GATAAACGGC TCTTTTGGAT TCAGTACCAT
    AGAGGAGGAG GTGATCCTCG TATTTGTTCC TGTGATTATG ACGGGGGTTC TGTCCTTTTT
    AGCAAATATT CCCTGAGGCA CAATCTGCTT GCAGAGTCCT TCTTTGGAGA TTGTATCTTC
    TATTCAACAT AGAAAAAGAA GACAATTTGG ATAGCTAACA AGCACACAGG GAAGGATATG
    GTTAAAATCA CCCTCAACTC GTCATTCGTG CCACCCAGTG GAATTAAAGT GGTTCGTCCA
    CTCGTATAGC CCAAGGCAGA GGGTGATGCT TGGGCATCTG ATGTCAGTGA GTGTGCTTTT
    CGGAATCATG GCTACACAGA TGACTGTGAA AATATCCCTG GATCCTATTA CTGCACATGC
    CCTGAGGGAT ATGTCCTGCT TCCTGATGGG AAATGGTGTC ACCAGTTAAT TTCCTGCCCA
    AGAAATGTGT CTAAATGCAG TCATGACTGT GTTCTGACAT CAGACCGTCC CATATGTTCC
    TGTCCTGAAG GCTCAGTGCT TGACACAGAT GGAAAGACAT GCAGTGGCTG TTCATCACCT
    GATAATGGGG GATGTAGCCA GCCCTGCACC CCTCTCAGCC CCGTATCCTG GAAATGTGGC
    TGCCTTCCTG GGTATGACCT ACAACTGGAC AAAAAGAGCC ATAGACCTTC AGGACCACCA
    CCATTTTTGC TGTTTGCCAG TTCTCAAGAC ATTCGATACA TGCATTTTGA TGGAACAGAT
    TATGGAACCC TGGTCTACCA ACAAATAATA GGAATGGTTT TTGCCCTTGA TCATGACCCT
    GTGGAAAATA AGATATACTT TGCCCATACA GTCCTGAAGT GGATAGAAAG AGCTAATATG
    GATGCTTCCC AGCGANGAAG GCTTATCGAG GAAGAAGTAG GTTTACCAGA AGGTCTTGCT
    GTTGACTGGA TTGGCCATAA ACTCTACTGG ACAGACAGAG GGAAATCTCT CATTGAAGGG
    AGTGATTTAA ATGGAAAGTA TTGTGAAATA ATCATTAAGG AGGGCATCTC TCAGCCACGA
    GGANATGCTG TTCATCCAAT GGCCAAGAGA TTATTCTGGA CTGATATAGG AATTAATCCA
    CGAATTGAAA GTTCTTCTCT TCAAGGCAGT GGCCGACTGG TTATAGCTAG CTCGGATCTA
    GTCTGGCACA GTGGAATAGC AGTTGATTAC TTAACTGACA AGTTGTATTG GTGCGATGCC
    AAGCAGTCTG TGATTGAAAT GTCCAATCTG GATGGCTCAA AATGCCAAAG ACTTCCCCAG
    AAGGATGTAG GTCACCCATT TGCCGTGGCT GTGTTTGAGG ATCACGTGTG GTTCTCTGAT
    TGGACTATGC CATCGATAAC AAGGATGAAC AAGAGGACTG GCAAAAATAG CGTACGTCTC
    CCAGGCAGCA TGCTGAAGCC CTCATCTCTG CTTGTAGTTC ATCCATTGGC AAAACCAGGA
    GCAGATCCCT GCTTACATCA AAATGGAGGC TGTGAACATA TTTGCAAAGA GAGGTTTGAA
    ACTGCTCAGT GTTTGTGTCA TGAAGGTTTT GTGAAAGCTC CAGATGGAAA AATGTGTCTG
    GCTCTTCATG GCCATCGGAT ATTGTCAGAT AATATGACAA ACTGTAGTGG AGCCAATCTG
    AGTAGTGAAG TCCTGCCACT GGACATCTTA GCCGGCAGCA GAGAGCTTGA CTATAACATG
    GCAGAGTCTC AACACCTGCC AGTGGCCGAG ATCATGGTGT CAGATTTGTT GAGAGCATGT
    AAACAGAAGC ATATGGCTTG TGGACCCTTT GATATAGATA CAGATTACAG ATCAACATGG
    TGTTTCTGTT TGGAGGTTAA CCTGTCCCAG AACTTGACAA AAGAAGTTCT AGAATGTTCT
    ATAATAAAGA CTGATGCCAT GGGATGCAGT ACACGGGCTG AGTGCCTTTC AAAGGGAGCA
    GATGTCACAT GTCAGTGTTT GAAAGGATTT GCTGGAGATG GAAAACAATG TTCTGATGTA
    GATGGATGTG AGATGGGTCT CTCAGTTTGC CCTCCCACCT CCCCCAAGTG TGTCAATACA
    GAAGGTGGCT ATGTCTGCTG GCGCTCAGAG AGCTACCAGG GAGATGGAAT CCACTGTTTG
    GATATTGATG AGTGCCGACG GGGCGTGCAC AGCTGTGGGG AAAATGCCAC CTGAAAAAAT
    ATGGAGGGAA ACCACACTTG CACGTGTGCT GGCGACTTGT CTGAGCCTGG ACAGATTTGC
    CCTGACTCTA CTCTGCTGTC TCACCTTGGG AAGAATGGAC ACAATTTTTT GAAAAAATGT
    TTCCCTGAAT ATACCCCGAA TTTTGAAGGG TACTGCCTCA ATGGTCATGT CTGTATATAT
    TTTGGCATTG CCAACCTGTT CTCCTGCCAC TGTCCCATTG GCTACCCTGG GAAGCGAGGT
    GAGTACATAG ACTTCGATGG GTGGGATCCG CACAGTGCAG GCCGTGGGCA TCAGTGGAAC
    ACCAGCCCGG TGGCTGTCCG TGCGCTGGTG CTGGCTTTCC TGCTGCTCCT CGGGCTGTGC
    AGAGCTCACT ACTACTACAG GACTCAGAAG TGGCTACTGA AAAATCCAAA GAATCCTTGT
    GAAGAACCGA GCAGAGATGT GATCCGTAGC AGGCCTGCAG ATGGCGAGGC TGGANTGCCT
    TTTTGCCCCC AACCTTGGTT CGTGGTTGTA AAGGAACATC AAAACCTCAG GAATGGGCGT
    CCATCCGAGG CCCTCAAGGA AGGCCTGGCA GCAGATGTTG GCCAGTTTTC CCCCCTGGAT
    CCTGGACCAG GGGTGAAGTG TAGCTTTCAT CTCCTCTCCG AAGGGGCATG GCCCATTGCT
    GAGGGGCTTG GGACATCCTG TCCTCTCCCA TCAGCTCACC CTTTGTGGCA ACAAAGATCT
    CCACACCAAA TGGAGCTGAC TCTGAAAACT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326994.1
    CDS1..3573
    Translation

    Target ORF information:

    RefSeq Version XM_015471508.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epidermal growth factor (EGF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTGCATT TCCTTGTCAT TGTGTTGCTG GTAGTTTTCA AATTTAGTTT TGTTAGCCTC 
    TCAGCCCTGC CGCACTTGAA CTGTCCTGAA GGCTCTCCCT CTGGAAATGG GAATTCTACT
    TGTGCAGGTC TTGTGCCCTT TTTAATTTTT TCCCATGGAA ATAGTATCTG TAGGATTGAT
    TTGGAAGGAA TGAATTATGA ACAATTGGTG GCAGATGCTG GCATCTCAGT GATCATGGAT
    TTTCATTACA ATGAAGAAAG AATCTATTGG GTTGATCTAN NCAGACAACT CTTGCAAAGA
    GTTCATTTGA ATGGGAGAAG GCAAGAGAAA GTATATGATA TAGATAAAAA TGTTTCAGGA
    ATGGCAATAA ATTGGATCAA TGAAGAACTT ATTTGGTCTA ATCAACAGGA AGGAATCATT
    ACAGTAACTG ATAGGAAAGG AAACAATTCC TGGGTTCTCT TAAGTGCCTT AAAATATCCT
    GCAAATATAG CAGTTGATCC AGTAGCAAGT TTTATGTTTT GGTCTTCAGA GGTGGCTNGC
    AGCCTTCACA GAGCAGATCT TGGTGGTATG GAAGTCAAGC TTCTGTTAGA GACATCAGAG
    AAAATAGCAG CTGTATCATT GGATATGCTT GATAAACGGC TCTTTTGGAT TCAGTACCAT
    AGAGGAGGAG GTGATCCTCG TATTTGTTCC TGTGATTATG ACGGGGGTTC TGTCCTTTTT
    AGCAAATATT CCCTGAGGCA CAATCTGCTT GCAGAGTCCT TCTTTGGAGA TTGTATCTTC
    TATTCAACAT AGAAAAAGAA GACAATTTGG ATAGCTAACA AGCACACAGG GAAGGATATG
    GTTAAAATCA CCCTCAACTC GTCATTCGTG CCACCCAGTG GAATTAAAGT GGTTCGTCCA
    CTCGTATAGC CCAAGGCAGA GGGTGATGCT TGGGCATCTG ATGTCAGTGA GTGTGCTTTT
    CGGAATCATG GCTACACAGA TGACTGTGAA AATATCCCTG GATCCTATTA CTGCACATGC
    CCTGAGGGAT ATGTCCTGCT TCCTGATGGG AAATGGTGTC ACCAGTTAAT TTCCTGCCCA
    AGAAATGTGT CTAAATGCAG TCATGACTGT GTTCTGACAT CAGACCGTCC CATATGTTCC
    TGTCCTGAAG GCTCAGTGCT TGACACAGAT GGAAAGACAT GCAGTGGCTG TTCATCACCT
    GATAATGGGG GATGTAGCCA GCCCTGCACC CCTCTCAGCC CCGTATCCTG GAAATGTGGC
    TGCCTTCCTG GGTATGACCT ACAACTGGAC AAAAAGAGCC ATAGACCTTC AGGACCACCA
    CCATTTTTGC TGTTTGCCAG TTCTCAAGAC ATTCGATACA TGCATTTTGA TGGAACAGAT
    TATGGAACCC TGGTCTACCA ACAAATAATA GGAATGGTTT TTGCCCTTGA TCATGACCCT
    GTGGAAAATA AGATATACTT TGCCCATACA GTCCTGAAGT GGATAGAAAG AGCTAATATG
    GATGCTTCCC AGCGANGAAG GCTTATCGAG GAAGAAGTAG GTTTACCAGA AGGTCTTGCT
    GTTGACTGGA TTGGCCATAA ACTCTACTGG ACAGACAGAG GGAAATCTCT CATTGAAGGG
    AGTGATTTAA ATGGAAAGTA TTGTGAAATA ATCATTAAGG AGGGCATCTC TCAGCCACGA
    GGANATGCTG TTCATCCAAT GGCCAAGAGA TTATTCTGGA CTGATATAGG AATTAATCCA
    CGAATTGAAA GTTCTTCTCT TCAAGGCAGT GGCCGACTGG TTATAGCTAG CTCGGATCTA
    GTCTGGCACA GTGGAATAGC AGTTGATTAC TTAACTGACA AGTTGTATTG GTGCGATGCC
    AAGCAGTCTG TGATTGAAAT GTCCAATCTG GATGGCTCAA AATGCCAAAG ACTTCCCCAG
    AAGGATGTAG GTCACCCATT TGCCGTGGCT GTGTTTGAGG ATCACGTGTG GTTCTCTGAT
    TGGACTATGC CATCGATAAC AAGGATGAAC AAGAGGACTG GCAAAAATAG CGTACGTCTC
    CCAGGCAGCA TGCTGAAGCC CTCATCTCTG CTTGTAGTTC ATCCATTGGC AAAACCAGGA
    GCAGATCCCT GCTTACATCA AAATGGAGGC TGTGAACATA TTTGCAAAGA GAGGTTTGAA
    ACTGCTCAGT GTTTGTGTCA TGAAGGTTTT GTGAAAGCTC CAGATGGAAA AATGTGTCTG
    GCTCTTCATG GCCATCGGAT ATTGTCAGAT AATATGACAA ACTGTAGTGG AGCCAATCTG
    AGTAGTGAAG TCCTGCCACT GGACATCTTA GCCGGCAGCA GAGAGCTTGA CTATAACATG
    GCAGAGTCTC AACACCTGCC AGTGGCCGAG ATCATGGTGT CAGATTTGTT GAGAGCATGT
    AAACAGAAGC ATATGGCTTG TGGACCCTTT GATATAGATA CAGATTACAG ATCAACATGG
    TGTTTCTGTT TGGAGGTTAA CCTGTCCCAG AACTTGACAA AAGAAGTTCT AGAATGTTCT
    ATAATAAAGA CTGATGCCAT GGGATGCAGT ACACGGGCTG AGTGCCTTTC AAAGGGAGCA
    GATGTCACAT GTCAGTGTTT GAAAGGATTT GCTGGAGATG GAAAACAATG TTCTGATGTA
    GATGGATGTG AGATGGGTCT CTCAGTTTGC CCTCCCACCT CCCCCAAGTG TGTCAATACA
    GAAGGTGGCT ATGTCTGCTG GCGCTCAGAG AGCTACCAGG GAGATGGAAT CCACTGTTTG
    GATATTGATG AGTGCCGACG GGGCGTGCAC AGCTGTGGGG AAAATGCCAC CTGAAAAAAT
    ATGGAGGGAA ACCACACTTG CACGTGTGCT GGCGACTTGT CTGAGCCTGG ACAGATTTGC
    CCTGACTCTA CTCTGCTGTC TCACCTTGGG AAGAATGGAC ACAATTTTTT GAAAAAATGT
    TTCCCTGAAT ATACCCCGAA TTTTGAAGGG TACTGCCTCA ATGGTCATGT CTGTATATAT
    TTTGGCATTG CCAACCTGTT CTCCTGCCAC TGTCCCATTG GCTACCCTGG GAAGCGAGGT
    GAGTACATAG ACTTCGATGG GTGGGATCCG CACAGTGCAG GCCGTGGGCA TCAGTGGAAC
    ACCAGCCCGG TGGCTGTCCG TGCGCTGGTG CTGGCTTTCC TGCTGCTCCT CGGGCTGTGC
    AGAGCTCACT ACTACTACAG GACTCAGAAG TGGCTACTGA AAAATCCAAA GAATCCTTGT
    GAAGAACCGA GCAGAGATGT GATCCGTAGC AGGCCTGCAG ATGGCGAGGC TGGANTGCCT
    TTTTGCCCCC AACCTTGGTT CGTGGTTGTA AAGGAACATC AAAACCTCAG GAATGGGCGT
    CCATCCGAGG CCCTCAAGGA AGGCCTGGCA GCAGATGTTG GCCAGTTTTC CCCCCTGGAT
    CCTGGACCAG GGGTGAAGTG TAGCTTTCAT CTCCTCTCCG AAGGGGCATG GCCCATTGCT
    GAGGGGCTTG GGACATCCTG TCCTCTCCCA TCAGCTCACC CTTTGTGGCA ACAAAGATCT
    CCACACCAAA TGGAGCTGAC TCTGAAAACT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.