LAMB3 cDNA ORF clone, Bos taurus(cattle)

The following LAMB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LAMB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223617 XM_005217367.4
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_015475274.2
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_010813565.3
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_015475277.2
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_010813564.3
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa93058 XM_015475281.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa35997 NM_001081596.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_010813564.2
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475276.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475277.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475274.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_005217367.3
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475275.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475279.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475278.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475280.1
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_010813565.2
Latest version!
Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223617
    Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
    Accession Version XM_015475277.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330763.2
    CDS312..3830
    Translation

    Target ORF information:

    RefSeq Version XM_015475277.2
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475277.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223617
    Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
    Accession Version XM_010813564.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811866.2
    CDS549..4067
    Translation

    Target ORF information:

    RefSeq Version XM_010813564.3
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813564.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223617
    Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
    Accession Version XM_010813565.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811867.2
    CDS597..4115
    Translation

    Target ORF information:

    RefSeq Version XM_010813565.3
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813565.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223617
    Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
    Accession Version XM_015475274.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330760.2
    CDS1104..4622
    Translation

    Target ORF information:

    RefSeq Version XM_015475274.2
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475274.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223617
    Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
    Accession Version XM_005217367.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217367.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217424.2
    CDS554..4072
    Translation

    Target ORF information:

    RefSeq Version XM_005217367.4
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217367.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93058
    Clone ID Related Accession (Same CDS sequence) XM_015475281.1
    Accession Version XM_015475281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACGGACGG AATGGACAGA GGTGGGCTGG TGAGTCGACA
    GTGAGTGGAT TCGAGGCTGT GGCCAGCTGC ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330767.1
    CDS542..3997
    Translation

    Target ORF information:

    RefSeq Version XM_015475281.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACGGACGG AATGGACAGA GGTGGGCTGG TGAGTCGACA
    GTGAGTGGAT TCGAGGCTGT GGCCAGCTGC ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35997
    Clone ID Related Accession (Same CDS sequence) NM_001081596.1
    Accession Version NM_001081596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133303.1. On Feb 20, 2007 this sequence version replaced XM_608401.3. ##Evidence-Data-START## Transcript exon combination :: BC133303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCGGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075065.1
    CDS81..3599
    Translation

    Target ORF information:

    RefSeq Version NM_001081596.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC
    TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCGGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_015475275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330761.1
    CDS848..4366
    Translation

    Target ORF information:

    RefSeq Version XM_015475275.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_015475276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330762.1
    CDS1391..4909
    Translation

    Target ORF information:

    RefSeq Version XM_015475276.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_015475277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330763.1
    CDS416..3934
    Translation

    Target ORF information:

    RefSeq Version XM_015475277.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_015475280.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330766.1
    CDS613..4131
    Translation

    Target ORF information:

    RefSeq Version XM_015475280.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475280.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_010813565.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811867.1
    CDS1768..5286
    Translation

    Target ORF information:

    RefSeq Version XM_010813565.2
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813565.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_015475274.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330760.1
    CDS1459..4977
    Translation

    Target ORF information:

    RefSeq Version XM_015475274.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475274.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_005217367.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217367.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217424.1
    CDS165..3683
    Translation

    Target ORF information:

    RefSeq Version XM_005217367.3
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217367.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_015475279.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330765.1
    CDS696..4214
    Translation

    Target ORF information:

    RefSeq Version XM_015475279.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475279.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_010813564.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811866.1
    CDS542..4060
    Translation

    Target ORF information:

    RefSeq Version XM_010813564.2
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813564.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27520
    Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
    Accession Version XM_015475278.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product laminin subunit beta-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330764.1
    CDS445..3963
    Translation

    Target ORF information:

    RefSeq Version XM_015475278.1
    Organism Bos taurus(cattle)
    Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
    TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT
    CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC
    GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC
    CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT
    GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG
    ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC
    AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC
    CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT
    AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC
    CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC
    TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT
    GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC
    CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC
    TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG
    CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA
    CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA
    GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG
    CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG
    TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG
    TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC
    ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG
    GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC
    AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC
    TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC
    GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC
    GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC
    ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG
    GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC
    TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC
    GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG
    GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT
    GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG
    ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA
    GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG
    GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA
    GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG
    CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA
    GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG
    ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC
    TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC
    GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG
    GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC
    TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG
    ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA
    GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG
    CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC
    AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC
    AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG
    GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC
    ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG
    GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA
    GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC
    CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA
    GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA
    CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG
    GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC
    GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC
    ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.