LAMA4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LAMA4
Entrez Gene ID	529670
Full Name	laminin subunit alpha 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024996681.1	XP_024852449.1	laminin subunit alpha-4 isoform X3
XM_024996678.1	XP_024852446.1	laminin subunit alpha-4 isoform X1
XM_024996679.1	XP_024852447.1	laminin subunit alpha-4 isoform X1
XM_024996680.1	XP_024852448.1	laminin subunit alpha-4 isoform X2
XM_024996682.1	XP_024852450.1	laminin subunit alpha-4 isoform X3
NM_001205965.1	NP_001192894.1	laminin subunit alpha-4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LAMA4	NP_001098676.2
	LAMA4	XP_002803983.1
Bos taurus (cattle)	LAMA4	NP_001192894.1
Danio rerio (zebrafish)	lama4	NP_001034154.1
Pan troglodytes (chimpanzee)	LAMA4	XP_518696.3
Canis lupus familiaris (dog)	LAMA4	XP_005627847.1
Rattus norvegicus (Norway rat)	Lama4	XP_003752023.1
Gallus gallus (chicken)	LAMA4	XP_004940355.1
Mus musculus (house mouse)	Lama4	NP_034811.2
Xenopus tropicalis (tropical clawed frog)	lama4	XP_002936983.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LAMA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LAMA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa237793	XM_024996682.1 Latest version!	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa237793	XM_024996681.1 Latest version!	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa237792	XM_024996680.1 Latest version!	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa01954	NM_001205965.1 Latest version!	Bos taurus laminin subunit alpha 4 (LAMA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa01954	XM_024996678.1 Latest version!	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa01954	XM_024996679.1 Latest version!	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LAMA4 ( XM_024996681.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852449.1 Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa237793
Clone ID Related Accession (Same CDS sequence)	XM_024996681.1 , XM_024996682.1
Accession Version	XM_024996681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit alpha-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGGTACA GTGCCCCTGC CGTTGTCACC CGACTGGGGC ACCTGCGCCC 
CGCGCTGCGT GTGCCACACT CGTTGTTCCC CTCCCCCGCC TTCCCTTTTC CACCCGCCTT 
GAGAGTTTCA CCTGGACGAG GTCAGTTCAG AAACTTGAAA TAAAGAGTTT TACTTTGTAC 
TTTGGATTTG GACTTTGTGA GTTGAGAGCG GAGACTTTGG AACACGTCCT TGTTCGAGCT 
GGCAAAAGAG CATGTGTCCT GAATACTCTG AGTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852449.1
CDS	238..696
Translation	MALSSAWCAVLPLWLLCGAACSRAASGDGSAFPFDIEGSSAFGRLDPPETSEPRVAPGSLPPAPKVQCPCRCHPTGAPAPRAACATLVVPLPRLPFSTRLESFTWTRSVQKLEIKSFTLYFGFGLCELRAETLEHVLVRAGKRACVLNTLSQ

Target ORF information:

RefSeq Version	XM_024996681.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996681.1

ORF Insert Sequence:

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGGTACA GTGCCCCTGC CGTTGTCACC CGACTGGGGC ACCTGCGCCC 
CGCGCTGCGT GTGCCACACT CGTTGTTCCC CTCCCCCGCC TTCCCTTTTC CACCCGCCTT 
GAGAGTTTCA CCTGGACGAG GTCAGTTCAG AAACTTGAAA TAAAGAGTTT TACTTTGTAC 
TTTGGATTTG GACTTTGTGA GTTGAGAGCG GAGACTTTGG AACACGTCCT TGTTCGAGCT 
GGCAAAAGAG CATGTGTCCT GAATACTCTG AGTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMA4 ( XM_024996682.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852450.1 Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa237793
Clone ID Related Accession (Same CDS sequence)	XM_024996681.1 , XM_024996682.1
Accession Version	XM_024996682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit alpha-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGGTACA GTGCCCCTGC CGTTGTCACC CGACTGGGGC ACCTGCGCCC 
CGCGCTGCGT GTGCCACACT CGTTGTTCCC CTCCCCCGCC TTCCCTTTTC CACCCGCCTT 
GAGAGTTTCA CCTGGACGAG GTCAGTTCAG AAACTTGAAA TAAAGAGTTT TACTTTGTAC 
TTTGGATTTG GACTTTGTGA GTTGAGAGCG GAGACTTTGG AACACGTCCT TGTTCGAGCT 
GGCAAAAGAG CATGTGTCCT GAATACTCTG AGTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852450.1
CDS	342..800
Translation	MALSSAWCAVLPLWLLCGAACSRAASGDGSAFPFDIEGSSAFGRLDPPETSEPRVAPGSLPPAPKVQCPCRCHPTGAPAPRAACATLVVPLPRLPFSTRLESFTWTRSVQKLEIKSFTLYFGFGLCELRAETLEHVLVRAGKRACVLNTLSQ

Target ORF information:

RefSeq Version	XM_024996682.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996682.1

ORF Insert Sequence:

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGGTACA GTGCCCCTGC CGTTGTCACC CGACTGGGGC ACCTGCGCCC 
CGCGCTGCGT GTGCCACACT CGTTGTTCCC CTCCCCCGCC TTCCCTTTTC CACCCGCCTT 
GAGAGTTTCA CCTGGACGAG GTCAGTTCAG AAACTTGAAA TAAAGAGTTT TACTTTGTAC 
TTTGGATTTG GACTTTGTGA GTTGAGAGCG GAGACTTTGG AACACGTCCT TGTTCGAGCT 
GGCAAAAGAG CATGTGTCCT GAATACTCTG AGTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMA4 ( XM_024996680.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852448.1 Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa237792
Clone ID Related Accession (Same CDS sequence)	XM_024996680.1
Accession Version	XM_024996680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit alpha-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCTGCGA TAAATGCATC TGGGATCTGA CTGATGACCT TCGGTTAGCA 
GCGCTCTCAA TTGAAGAAAG CAAATCCGGC CTGCTGAGTG TGTCATCTGG TGCCGCAGCT 
CATAGGCACG TGAACGAAAT CAACTCCACC ATCTACCTCC TCAAAACAAA ATTGTCAGAA 
AGAGAAAACC AGTATGTCCT AAGAAAGATA CAAATCAACA ATGCTGAAAA CACAATGAAA 
AGCCTTCAGT CTGACATGGA GGAATTAGCT GAAAGGGAAA GCCAAGCTTC AAGAAAGGTC 
CAGTTGGCTC AGAAGGAAAG CATGGATACC ATTAACCATG CAACTCAGCT TGCAGAGCAA 
GCCCACAATA TGAGGGATAA AATCCAAGAG ATCAGCAGCA AGATGCTGTA TTATGGGGAA 
GAGCAGGAAC TTAGCTCTGA GGAAATCTCC GAGAAGCTGG TGTTGGCACA GAAGATGCTT 
GAGGAGATCC GACGCCGTCA GCCGTTCCTC ACCCAACGGG AACTTGTGGA CGAGGAGGCA 
GACGAAGCCC ATGAATTGCT GAGCCAGGCT GAGAGCTGGC AGAGGCAGTA CAATGACACT 
CGCTCTCTGT TTCCTGTGGT CCTGGAGCAG CTGGACGACT ATAACGCCAA GCTGTCAGAC 
CTCCAGGAAT CACTCGACCA GGCCCTTAAC CATGTCAGGG ATGCTGAAGA CATGAACAGA 
GCCACAGCAG CCAGGCAGCG GGACCACGAG AAACAGCATG AGAGAGTAAG GGAACAGACG 
GAAGGGGTGA ACGCGTCTCT GAGGACGTCT TCCAACTCAC TGATGATGCC ACGACTGACC 
CTGTCAGAAC TTGATAGCAT AATAAAGAAT GCATCAGGGA TTTATGCCGA AATAGATGGA 
GCCAAAAATG AACTTCAAAG AAAACTATCC AACCTGAGCA ACCTCAGTCA TGATTTAGTT 
CAGGAAGCTG TTGACCATGC ACGGAACCTT CAGCAAGAGG CTGATGAGTT GAGCAGGAAT 
TTGCACAGTT CAGATATGAA TGGACTGGTA CAGAAGGCTT TGGATGCTTC AAATGTCTAT 
GAGAATATTG CCAATTACGT TAGTGAAGCC AATGAAACAG CAGAACAGGC TTTGAACATC 
ACTGATCGAA TTTATGATGC TGTGAGTGGG ATTGATACAC AGATCATTTA CCATAAAGAT 
GAGAGCGAGA ACCTCCTCAA TCAAGCCAGA GAGCTGCAAG CAAGGGCAGA TTCTAGCAGT 
GATGAGGCAG TGGTGGATAC CAGCAGGCGC GTGGGAGGAG CCCTAGCAAG GAAGAGTGCT 
CTCAGAGACA GATTAAATGA TGCCATGAAA AGACTGCAGG CCGCAGAGAG AGGTGATGCC 
CAGCAGCGCC TGGGTCAGTC CCAGCTGATC ACCGCGGAAG CTAACAAGAC CACAATGGAA 
GTCCAGGAGG CAACCGCCCC GATGGCTAGC AACCTAACCA ACTGGTCACA GAATCTCCAA 
AGTTTTGACT CTTCTGCTTA CAACACTGCC GTGGACTCTG CCAGAGATGC AGTTGGAAAC 
CTGACAGAGG TTGTCCCTCA GCTCCTGGAT CAGCTTCGTA CGGTAGAACA GAAGCGGCCT 
GCAAGCAATG TTTCCGCCAG CATCCAAAGG ATCCGAGAGC TCATTGCTCA GACCAGAAGT 
GTTGCCAGCA AGATCCAAGT CTCCATGATG TTTGATGGCC AGTCAGCAGT CGAGGTGCAC 
CCCAGAGCCA GCATGGATGA CTTAAAGACC TTTACATCTC TGAGCCTGTA CATGAAGCCT 
CCTCCTGTGA AGCAGTCGGA ACTGGCTGTG ACTGCAGATC AGTTCATCCT GTACCTCGGA 
AGCAAAAACG CCAAAAAAGA GTACATGGGT CTTGCAATCA AAAATGACAA CCTGGTGTAC 
GTTTATAATT TGGGAACTAA GGATGTAGAG ATTCCCTTGG ACTCGAAGCC TGTCAGTACC 
TGGCCTGCTT ACTTCAGCAT TGTCAAGATT GAAAGGGTAG GAAAACATGG AAAGGTGTTT 
TTAACAGTCC CAAGTCTAAG TAGCACTGCA GAGGAAAAGT TTATTAAAAA GGGAGAATTT 
GCTGGAGATG ACTCCTTGTT GGATCTGGAT CCCGAAGATA CTGTGTTTTA TGTTGGGGGC 
GTGCCATCAA ACTTCAAGCT CCCTGCTAGC TTAAACCTGC CTGGCTTTGT TGGCTGCCTG 
GAATTGGCCA CTTTGAATAA TGATGTGATC AGCTTGTACA ACTTTAAGCA CATCTATAAT 
ATGGACCCCT CCAAATCAGT GCCCTGTGCC AGAGACAAAC TGGCCTTTAC GCAGAGTCGG 
GCTGCCAGCT ATTTCTTCGA TGGCTCTAGT TATGCCATAG TGAGGGATAT CACAAGGAGA 
GGGAAGTTTG GTCAGGTGAC TCGCTTTGAC ATCGAAGTCA GAACACCAGC TGACAATGGC 
CTCGTGCTCC TCATGGTCAA TGGAAGTATG TTTTTCAGCT TGGAAATGCG CAGTGGTTAC 
CTACATGTGT TCTATGACTT TGGATTTAGC AACGGCCCTG TGCATCTTGA AGACACGTTA 
AAGAAAGCTC AAATTAATGA TGCAAAATAC CATGAGATCT CAATCATTTA CCACAATGAT 
AAGAAAATGA TTTTGGTAGT TGACAGACGA CATGTCAAGA GCATGGATAA TGAAAAGATG 
AAGATACCTT TTACAGACAT ATACATAGGT GGAGCTCCCC CAGAAATCTT ACAATCAAGG 
ACCTTAAGAG CACATCTTCC CCTAGATATC AACTTCAGAG GATGCATGAA GGGCTTCCAG 
TTCCAAAAGA AAGATTTCAA TTTATTAGAA CAGACAGAAA CCCTGGGAGT TGGCTATGGA 
TGCCCAGAAG ACTCTCTTAT ATCTCGCAGA GCATATTTCA ATGGACAGAG TTACATTGCT 
TCAAGTCAGA AAATATCTTT TTTTGATGGC TTTGAAGGAG GTTTTAATTT CCGAACATTA 
CAGCCAAATG GATTACTGTT CTATTATGCA TCAGGGTCAG ACATGTTCTC CATCTCACTG 
AATAATGGCA CCGTAATCAT GGATGTAAAG GGAATCAAGG TGCAGTCGGC AGATAAGCAG 
TACAATGATG GGCTGTCCCA CTTCATCATC ACCTCCGTCT CCCCAGCAAG GTATGAATTG 
ATAGTAGATA AAAGCAGACT TGGGAGTAAG AACCCTACCA AAGGGAAAGT GGAGCAGACC 
CAAGCAGATG AAAAGAAGTT CTACTTCGGC GGCTCACCCA TCACTCCCCA GTATGCTAAT 
TTCACCGGAT GTATAAGTAA TGCCTACTTT ACCAGGTTGG ATAGAGATGT AGAGGTCGAA 
GATTTCCAGC GGTATTCTGA AAAGGTCCAC ACTTCTCTTT ATGAGTGTCC CATTGAGTCT 
TCACCATTGT TTCTCCTTCA CAAAAAAGGA AGAAATTCCT CAAAGCCTAA AACAAATCAG 
AATAAAAAGG GAGAGAAAAG TAAAGATGCT CCTTCGTGGG ATCCTGTTGG CCTGAAATTC 
CTAGAGAGGA ATATTCCAAG AGACTCACAC TGCCAGCTTT CCAACAGCCC TAGAGCGATA 
GAGCACGCCT ATCAGTATGG AGGGACAGCC AACAGTCGTC AAGAGTTTGA ACACCTAAAA 
AGAGATTTTG GTGAAAAATC CCAGTTTTCC ATTCGTCTGA AAACACGTTC CTCCCATGGG 
ATGATTTTCT ATGTCTCAGA TCAAGAAGAG AATAACTTCA TGACTCTGTT CTTAGCCCAT 
GGCCGCTTGG TTTTCATGTT TAATGTTGGC CACAAGAAAC TGAAGATTAG AAGCCAGGAG 
AAATACAATG ATGGACTGTG GCATGATGTG ATATTTATTC GGGAAAAGAG CAGCGGCCGA 
ATGATAATTG ATGGGCTTCG AGTCTTAGAA GAAAGTCTTC CCCCTACAGG AGCTGACTGG 
AAAATCAGGG GTCCTATTTA TTTGGGAGGT GTGGCTCCTG GAAGGGCTGT GAAAAATGTC 
CAGATAAACT CAGTCTACAG CTTCAGTGGC TGCCTCAGCA ATCTTCAGCT CAATGGGGCC 
TCCATCACCT CTGCTTCTCA GACATTTAGT GTGACTCCTT GTTTCGAAGG TCCAATGGAA 
ACAGGAACAT ACTTCTCAAC AGAAGGAGGT TACGTGGTCC TAGATGAGTC TTTCAATATT 
GGATTGAAGT TTGAGATTGC ATTTGAAGTC CGTCCCAGAA GCAGTTCCGG AACCCTTGTT 
CACGGCCACA GTGTCAATGG AGAATACCTA AATGTTCACA TGAAAAATGG GCAGGTCATA 
GTGAAAGTCA ACAATGGCAT CAGAGACTTT TCCACCTCAG TGACACCCAA GCAGAGTCTC 
TGTGATGGCA GATGGCACAG AATTACAGTT ATTAGAGATT CAAATGTGGT TCAGTTGGAT 
GTAGACTCTG AAGTGAACCA CGTGGTTGGA CCCCTAAATC CAAAACCAGT TGATCACAGG 
GAGCCTGTGT TTGTTGGAGG TGTTCCAGAG TCTCTACTGA CACCACGCTT GGCCCCCGGC 
AGACCCTTCA CAGGCTGCAT CCGTCACTTT GTGATTGACG GGCGCCCGGT GAGCTTCAGT 
AAAGCAGCCC TGGTCAGCGG CGCCGTGAGC ATCAACTCCT GTCCAGCAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852448.1
CDS	198..5651
Translation	MALSSAWCAVLPLWLLCGAACSRAASGDGSAFPFDIEGSSAFGRLDPPETSEPRVAPGSLPPAPKKCDAGFFFAYSEECLPCDCNGNSNECLDGSGLCVHCQRNTTGEHCEKCLDGYIGDAIRGPPRFCQPCPCPLPHVANFAESCYRKNGAVRCICKENYAGPNCERCAPGYYGNPLLIGSTCKKCDCSGNSDPNLIFEDCDEVTGQCRNCLRNTTGFKCERCAPGYYGDARRAKNCAACNCGGGPCDSITGECLEEGFESPTGCDKCIWDLTDDLRLAALSIEESKSGLLSVSSGAAAHRHVNEINSTIYLLKTKLSERENQYVLRKIQINNAENTMKSLQSDMEELAERESQASRKVQLAQKESMDTINHATQLAEQAHNMRDKIQEISSKMLYYGEEQELSSEEISEKLVLAQKMLEEIRRRQPFLTQRELVDEEADEAHELLSQAESWQRQYNDTRSLFPVVLEQLDDYNAKLSDLQESLDQALNHVRDAEDMNRATAARQRDHEKQHERVREQTEGVNASLRTSSNSLMMPRLTLSELDSIIKNASGIYAEIDGAKNELQRKLSNLSNLSHDLVQEAVDHARNLQQEADELSRNLHSSDMNGLVQKALDASNVYENIANYVSEANETAEQALNITDRIYDAVSGIDTQIIYHKDESENLLNQARELQARADSSSDEAVVDTSRRVGGALARKSALRDRLNDAMKRLQAAERGDAQQRLGQSQLITAEANKTTMEVQEATAPMASNLTNWSQNLQSFDSSAYNTAVDSARDAVGNLTEVVPQLLDQLRTVEQKRPASNVSASIQRIRELIAQTRSVASKIQVSMMFDGQSAVEVHPRASMDDLKTFTSLSLYMKPPPVKQSELAVTADQFILYLGSKNAKKEYMGLAIKNDNLVYVYNLGTKDVEIPLDSKPVSTWPAYFSIVKIERVGKHGKVFLTVPSLSSTAEEKFIKKGEFAGDDSLLDLDPEDTVFYVGGVPSNFKLPASLNLPGFVGCLELATLNNDVISLYNFKHIYNMDPSKSVPCARDKLAFTQSRAASYFFDGSSYAIVRDITRRGKFGQVTRFDIEVRTPADNGLVLLMVNGSMFFSLEMRSGYLHVFYDFGFSNGPVHLEDTLKKAQINDAKYHEISIIYHNDKKMILVVDRRHVKSMDNEKMKIPFTDIYIGGAPPEILQSRTLRAHLPLDINFRGCMKGFQFQKKDFNLLEQTETLGVGYGCPEDSLISRRAYFNGQSYIASSQKISFFDGFEGGFNFRTLQPNGLLFYYASGSDMFSISLNNGTVIMDVKGIKVQSADKQYNDGLSHFIITSVSPARYELIVDKSRLGSKNPTKGKVEQTQADEKKFYFGGSPITPQYANFTGCISNAYFTRLDRDVEVEDFQRYSEKVHTSLYECPIESSPLFLLHKKGRNSSKPKTNQNKKGEKSKDAPSWDPVGLKFLERNIPRDSHCQLSNSPRAIEHAYQYGGTANSRQEFEHLKRDFGEKSQFSIRLKTRSSHGMIFYVSDQEENNFMTLFLAHGRLVFMFNVGHKKLKIRSQEKYNDGLWHDVIFIREKSSGRMIIDGLRVLEESLPPTGADWKIRGPIYLGGVAPGRAVKNVQINSVYSFSGCLSNLQLNGASITSASQTFSVTPCFEGPMETGTYFSTEGGYVVLDESFNIGLKFEIAFEVRPRSSSGTLVHGHSVNGEYLNVHMKNGQVIVKVNNGIRDFSTSVTPKQSLCDGRWHRITVIRDSNVVQLDVDSEVNHVVGPLNPKPVDHREPVFVGGVPESLLTPRLAPGRPFTGCIRHFVIDGRPVSFSKAALVSGAVSINSCPAA

Target ORF information:

RefSeq Version	XM_024996680.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996680.1

ORF Insert Sequence:

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCTGCGA TAAATGCATC TGGGATCTGA CTGATGACCT TCGGTTAGCA 
GCGCTCTCAA TTGAAGAAAG CAAATCCGGC CTGCTGAGTG TGTCATCTGG TGCCGCAGCT 
CATAGGCACG TGAACGAAAT CAACTCCACC ATCTACCTCC TCAAAACAAA ATTGTCAGAA 
AGAGAAAACC AGTATGTCCT AAGAAAGATA CAAATCAACA ATGCTGAAAA CACAATGAAA 
AGCCTTCAGT CTGACATGGA GGAATTAGCT GAAAGGGAAA GCCAAGCTTC AAGAAAGGTC 
CAGTTGGCTC AGAAGGAAAG CATGGATACC ATTAACCATG CAACTCAGCT TGCAGAGCAA 
GCCCACAATA TGAGGGATAA AATCCAAGAG ATCAGCAGCA AGATGCTGTA TTATGGGGAA 
GAGCAGGAAC TTAGCTCTGA GGAAATCTCC GAGAAGCTGG TGTTGGCACA GAAGATGCTT 
GAGGAGATCC GACGCCGTCA GCCGTTCCTC ACCCAACGGG AACTTGTGGA CGAGGAGGCA 
GACGAAGCCC ATGAATTGCT GAGCCAGGCT GAGAGCTGGC AGAGGCAGTA CAATGACACT 
CGCTCTCTGT TTCCTGTGGT CCTGGAGCAG CTGGACGACT ATAACGCCAA GCTGTCAGAC 
CTCCAGGAAT CACTCGACCA GGCCCTTAAC CATGTCAGGG ATGCTGAAGA CATGAACAGA 
GCCACAGCAG CCAGGCAGCG GGACCACGAG AAACAGCATG AGAGAGTAAG GGAACAGACG 
GAAGGGGTGA ACGCGTCTCT GAGGACGTCT TCCAACTCAC TGATGATGCC ACGACTGACC 
CTGTCAGAAC TTGATAGCAT AATAAAGAAT GCATCAGGGA TTTATGCCGA AATAGATGGA 
GCCAAAAATG AACTTCAAAG AAAACTATCC AACCTGAGCA ACCTCAGTCA TGATTTAGTT 
CAGGAAGCTG TTGACCATGC ACGGAACCTT CAGCAAGAGG CTGATGAGTT GAGCAGGAAT 
TTGCACAGTT CAGATATGAA TGGACTGGTA CAGAAGGCTT TGGATGCTTC AAATGTCTAT 
GAGAATATTG CCAATTACGT TAGTGAAGCC AATGAAACAG CAGAACAGGC TTTGAACATC 
ACTGATCGAA TTTATGATGC TGTGAGTGGG ATTGATACAC AGATCATTTA CCATAAAGAT 
GAGAGCGAGA ACCTCCTCAA TCAAGCCAGA GAGCTGCAAG CAAGGGCAGA TTCTAGCAGT 
GATGAGGCAG TGGTGGATAC CAGCAGGCGC GTGGGAGGAG CCCTAGCAAG GAAGAGTGCT 
CTCAGAGACA GATTAAATGA TGCCATGAAA AGACTGCAGG CCGCAGAGAG AGGTGATGCC 
CAGCAGCGCC TGGGTCAGTC CCAGCTGATC ACCGCGGAAG CTAACAAGAC CACAATGGAA 
GTCCAGGAGG CAACCGCCCC GATGGCTAGC AACCTAACCA ACTGGTCACA GAATCTCCAA 
AGTTTTGACT CTTCTGCTTA CAACACTGCC GTGGACTCTG CCAGAGATGC AGTTGGAAAC 
CTGACAGAGG TTGTCCCTCA GCTCCTGGAT CAGCTTCGTA CGGTAGAACA GAAGCGGCCT 
GCAAGCAATG TTTCCGCCAG CATCCAAAGG ATCCGAGAGC TCATTGCTCA GACCAGAAGT 
GTTGCCAGCA AGATCCAAGT CTCCATGATG TTTGATGGCC AGTCAGCAGT CGAGGTGCAC 
CCCAGAGCCA GCATGGATGA CTTAAAGACC TTTACATCTC TGAGCCTGTA CATGAAGCCT 
CCTCCTGTGA AGCAGTCGGA ACTGGCTGTG ACTGCAGATC AGTTCATCCT GTACCTCGGA 
AGCAAAAACG CCAAAAAAGA GTACATGGGT CTTGCAATCA AAAATGACAA CCTGGTGTAC 
GTTTATAATT TGGGAACTAA GGATGTAGAG ATTCCCTTGG ACTCGAAGCC TGTCAGTACC 
TGGCCTGCTT ACTTCAGCAT TGTCAAGATT GAAAGGGTAG GAAAACATGG AAAGGTGTTT 
TTAACAGTCC CAAGTCTAAG TAGCACTGCA GAGGAAAAGT TTATTAAAAA GGGAGAATTT 
GCTGGAGATG ACTCCTTGTT GGATCTGGAT CCCGAAGATA CTGTGTTTTA TGTTGGGGGC 
GTGCCATCAA ACTTCAAGCT CCCTGCTAGC TTAAACCTGC CTGGCTTTGT TGGCTGCCTG 
GAATTGGCCA CTTTGAATAA TGATGTGATC AGCTTGTACA ACTTTAAGCA CATCTATAAT 
ATGGACCCCT CCAAATCAGT GCCCTGTGCC AGAGACAAAC TGGCCTTTAC GCAGAGTCGG 
GCTGCCAGCT ATTTCTTCGA TGGCTCTAGT TATGCCATAG TGAGGGATAT CACAAGGAGA 
GGGAAGTTTG GTCAGGTGAC TCGCTTTGAC ATCGAAGTCA GAACACCAGC TGACAATGGC 
CTCGTGCTCC TCATGGTCAA TGGAAGTATG TTTTTCAGCT TGGAAATGCG CAGTGGTTAC 
CTACATGTGT TCTATGACTT TGGATTTAGC AACGGCCCTG TGCATCTTGA AGACACGTTA 
AAGAAAGCTC AAATTAATGA TGCAAAATAC CATGAGATCT CAATCATTTA CCACAATGAT 
AAGAAAATGA TTTTGGTAGT TGACAGACGA CATGTCAAGA GCATGGATAA TGAAAAGATG 
AAGATACCTT TTACAGACAT ATACATAGGT GGAGCTCCCC CAGAAATCTT ACAATCAAGG 
ACCTTAAGAG CACATCTTCC CCTAGATATC AACTTCAGAG GATGCATGAA GGGCTTCCAG 
TTCCAAAAGA AAGATTTCAA TTTATTAGAA CAGACAGAAA CCCTGGGAGT TGGCTATGGA 
TGCCCAGAAG ACTCTCTTAT ATCTCGCAGA GCATATTTCA ATGGACAGAG TTACATTGCT 
TCAAGTCAGA AAATATCTTT TTTTGATGGC TTTGAAGGAG GTTTTAATTT CCGAACATTA 
CAGCCAAATG GATTACTGTT CTATTATGCA TCAGGGTCAG ACATGTTCTC CATCTCACTG 
AATAATGGCA CCGTAATCAT GGATGTAAAG GGAATCAAGG TGCAGTCGGC AGATAAGCAG 
TACAATGATG GGCTGTCCCA CTTCATCATC ACCTCCGTCT CCCCAGCAAG GTATGAATTG 
ATAGTAGATA AAAGCAGACT TGGGAGTAAG AACCCTACCA AAGGGAAAGT GGAGCAGACC 
CAAGCAGATG AAAAGAAGTT CTACTTCGGC GGCTCACCCA TCACTCCCCA GTATGCTAAT 
TTCACCGGAT GTATAAGTAA TGCCTACTTT ACCAGGTTGG ATAGAGATGT AGAGGTCGAA 
GATTTCCAGC GGTATTCTGA AAAGGTCCAC ACTTCTCTTT ATGAGTGTCC CATTGAGTCT 
TCACCATTGT TTCTCCTTCA CAAAAAAGGA AGAAATTCCT CAAAGCCTAA AACAAATCAG 
AATAAAAAGG GAGAGAAAAG TAAAGATGCT CCTTCGTGGG ATCCTGTTGG CCTGAAATTC 
CTAGAGAGGA ATATTCCAAG AGACTCACAC TGCCAGCTTT CCAACAGCCC TAGAGCGATA 
GAGCACGCCT ATCAGTATGG AGGGACAGCC AACAGTCGTC AAGAGTTTGA ACACCTAAAA 
AGAGATTTTG GTGAAAAATC CCAGTTTTCC ATTCGTCTGA AAACACGTTC CTCCCATGGG 
ATGATTTTCT ATGTCTCAGA TCAAGAAGAG AATAACTTCA TGACTCTGTT CTTAGCCCAT 
GGCCGCTTGG TTTTCATGTT TAATGTTGGC CACAAGAAAC TGAAGATTAG AAGCCAGGAG 
AAATACAATG ATGGACTGTG GCATGATGTG ATATTTATTC GGGAAAAGAG CAGCGGCCGA 
ATGATAATTG ATGGGCTTCG AGTCTTAGAA GAAAGTCTTC CCCCTACAGG AGCTGACTGG 
AAAATCAGGG GTCCTATTTA TTTGGGAGGT GTGGCTCCTG GAAGGGCTGT GAAAAATGTC 
CAGATAAACT CAGTCTACAG CTTCAGTGGC TGCCTCAGCA ATCTTCAGCT CAATGGGGCC 
TCCATCACCT CTGCTTCTCA GACATTTAGT GTGACTCCTT GTTTCGAAGG TCCAATGGAA 
ACAGGAACAT ACTTCTCAAC AGAAGGAGGT TACGTGGTCC TAGATGAGTC TTTCAATATT 
GGATTGAAGT TTGAGATTGC ATTTGAAGTC CGTCCCAGAA GCAGTTCCGG AACCCTTGTT 
CACGGCCACA GTGTCAATGG AGAATACCTA AATGTTCACA TGAAAAATGG GCAGGTCATA 
GTGAAAGTCA ACAATGGCAT CAGAGACTTT TCCACCTCAG TGACACCCAA GCAGAGTCTC 
TGTGATGGCA GATGGCACAG AATTACAGTT ATTAGAGATT CAAATGTGGT TCAGTTGGAT 
GTAGACTCTG AAGTGAACCA CGTGGTTGGA CCCCTAAATC CAAAACCAGT TGATCACAGG 
GAGCCTGTGT TTGTTGGAGG TGTTCCAGAG TCTCTACTGA CACCACGCTT GGCCCCCGGC 
AGACCCTTCA CAGGCTGCAT CCGTCACTTT GTGATTGACG GGCGCCCGGT GAGCTTCAGT 
AAAGCAGCCC TGGTCAGCGG CGCCGTGAGC ATCAACTCCT GTCCAGCAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMA4 ( NM_001205965.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192894.1 Bos taurus laminin subunit alpha 4 (LAMA4), mRNA.

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CloneID	OBa01954
Clone ID Related Accession (Same CDS sequence)	XM_024996678.1 , XM_024996679.1 , NM_001205965.1
Accession Version	NM_001205965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	laminin subunit alpha-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000009.1. On Apr 20, 2011 this sequence version replaced XM_608123.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5765710.1999.1, SRR5721694.1555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCACGGA CTGCCCAACC ATAAGCTGCG ATAAATGCAT CTGGGATCTG 
ACTGATGACC TTCGGTTAGC AGCGCTCTCA ATTGAAGAAA GCAAATCCGG CCTGCTGAGT 
GTGTCATCTG GTGCCGCAGC TCATAGGCAC GTGAACGAAA TCAACTCCAC CATCTACCTC 
CTCAAAACAA AATTGTCAGA AAGAGAAAAC CAGTATGTCC TAAGAAAGAT ACAAATCAAC 
AATGCTGAAA ACACAATGAA AAGCCTTCAG TCTGACATGG AGGAATTAGC TGAAAGGGAA 
AGCCAAGCTT CAAGAAAGGT CCAGTTGGCT CAGAAGGAAA GCATGGATAC CATTAACCAT 
GCAACTCAGC TTGCAGAGCA AGCCCACAAT ATGAGGGATA AAATCCAAGA GATCAGCAGC 
AAGATGCTGT ATTATGGGGA AGAGCAGGAA CTTAGCTCTG AGGAAATCTC CGAGAAGCTG 
GTGTTGGCAC AGAAGATGCT TGAGGAGATC CGACGCCGTC AGCCGTTCCT CACCCAACGG 
GAACTTGTGG ACGAGGAGGC AGACGAAGCC CATGAATTGC TGAGCCAGGC TGAGAGCTGG 
CAGAGGCAGT ACAATGACAC TCGCTCTCTG TTTCCTGTGG TCCTGGAGCA GCTGGACGAC 
TATAACGCCA AGCTGTCAGA CCTCCAGGAA TCACTCGACC AGGCCCTTAA CCATGTCAGG 
GATGCTGAAG ACATGAACAG AGCCACAGCA GCCAGGCAGC GGGACCACGA GAAACAGCAT 
GAGAGAGTAA GGGAACAGAC GGAAGGGGTG AACGCGTCTC TGAGGACGTC TTCCAACTCA 
CTGATGATGC CACGACTGAC CCTGTCAGAA CTTGATAGCA TAATAAAGAA TGCATCAGGG 
ATTTATGCCG AAATAGATGG AGCCAAAAAT GAACTTCAAA GAAAACTATC CAACCTGAGC 
AACCTCAGTC ATGATTTAGT TCAGGAAGCT GTTGACCATG CACGGAACCT TCAGCAAGAG 
GCTGATGAGT TGAGCAGGAA TTTGCACAGT TCAGATATGA ATGGACTGGT ACAGAAGGCT 
TTGGATGCTT CAAATGTCTA TGAGAATATT GCCAATTACG TTAGTGAAGC CAATGAAACA 
GCAGAACAGG CTTTGAACAT CACTGATCGA ATTTATGATG CTGTGAGTGG GATTGATACA 
CAGATCATTT ACCATAAAGA TGAGAGCGAG AACCTCCTCA ATCAAGCCAG AGAGCTGCAA 
GCAAGGGCAG ATTCTAGCAG TGATGAGGCA GTGGTGGATA CCAGCAGGCG CGTGGGAGGA 
GCCCTAGCAA GGAAGAGTGC TCTCAGAGAC AGATTAAATG ATGCCATGAA AAGACTGCAG 
GCCGCAGAGA GAGGTGATGC CCAGCAGCGC CTGGGTCAGT CCCAGCTGAT CACCGCGGAA 
GCTAACAAGA CCACAATGGA AGTCCAGGAG GCAACCGCCC CGATGGCTAG CAACCTAACC 
AACTGGTCAC AGAATCTCCA AAGTTTTGAC TCTTCTGCTT ACAACACTGC CGTGGACTCT 
GCCAGAGATG CAGTTGGAAA CCTGACAGAG GTTGTCCCTC AGCTCCTGGA TCAGCTTCGT 
ACGGTAGAAC AGAAGCGGCC TGCAAGCAAT GTTTCCGCCA GCATCCAAAG GATCCGAGAG 
CTCATTGCTC AGACCAGAAG TGTTGCCAGC AAGATCCAAG TCTCCATGAT GTTTGATGGC 
CAGTCAGCAG TCGAGGTGCA CCCCAGAGCC AGCATGGATG ACTTAAAGAC CTTTACATCT 
CTGAGCCTGT ACATGAAGCC TCCTCCTGTG AAGCAGTCGG AACTGGCTGT GACTGCAGAT 
CAGTTCATCC TGTACCTCGG AAGCAAAAAC GCCAAAAAAG AGTACATGGG TCTTGCAATC 
AAAAATGACA ACCTGGTGTA CGTTTATAAT TTGGGAACTA AGGATGTAGA GATTCCCTTG 
GACTCGAAGC CTGTCAGTAC CTGGCCTGCT TACTTCAGCA TTGTCAAGAT TGAAAGGGTA 
GGAAAACATG GAAAGGTGTT TTTAACAGTC CCAAGTCTAA GTAGCACTGC AGAGGAAAAG 
TTTATTAAAA AGGGAGAATT TGCTGGAGAT GACTCCTTGT TGGATCTGGA TCCCGAAGAT 
ACTGTGTTTT ATGTTGGGGG CGTGCCATCA AACTTCAAGC TCCCTGCTAG CTTAAACCTG 
CCTGGCTTTG TTGGCTGCCT GGAATTGGCC ACTTTGAATA ATGATGTGAT CAGCTTGTAC 
AACTTTAAGC ACATCTATAA TATGGACCCC TCCAAATCAG TGCCCTGTGC CAGAGACAAA 
CTGGCCTTTA CGCAGAGTCG GGCTGCCAGC TATTTCTTCG ATGGCTCTAG TTATGCCATA 
GTGAGGGATA TCACAAGGAG AGGGAAGTTT GGTCAGGTGA CTCGCTTTGA CATCGAAGTC 
AGAACACCAG CTGACAATGG CCTCGTGCTC CTCATGGTCA ATGGAAGTAT GTTTTTCAGC 
TTGGAAATGC GCAGTGGTTA CCTACATGTG TTCTATGACT TTGGATTTAG CAACGGCCCT 
GTGCATCTTG AAGACACGTT AAAGAAAGCT CAAATTAATG ATGCAAAATA CCATGAGATC 
TCAATCATTT ACCACAATGA TAAGAAAATG ATTTTGGTAG TTGACAGACG ACATGTCAAG 
AGCATGGATA ATGAAAAGAT GAAGATACCT TTTACAGACA TATACATAGG TGGAGCTCCC 
CCAGAAATCT TACAATCAAG GACCTTAAGA GCACATCTTC CCCTAGATAT CAACTTCAGA 
GGATGCATGA AGGGCTTCCA GTTCCAAAAG AAAGATTTCA ATTTATTAGA ACAGACAGAA 
ACCCTGGGAG TTGGCTATGG ATGCCCAGAA GACTCTCTTA TATCTCGCAG AGCATATTTC 
AATGGACAGA GTTACATTGC TTCAAGTCAG AAAATATCTT TTTTTGATGG CTTTGAAGGA 
GGTTTTAATT TCCGAACATT ACAGCCAAAT GGATTACTGT TCTATTATGC ATCAGGGTCA 
GACATGTTCT CCATCTCACT GAATAATGGC ACCGTAATCA TGGATGTAAA GGGAATCAAG 
GTGCAGTCGG CAGATAAGCA GTACAATGAT GGGCTGTCCC ACTTCATCAT CACCTCCGTC 
TCCCCAGCAA GGTATGAATT GATAGTAGAT AAAAGCAGAC TTGGGAGTAA GAACCCTACC 
AAAGGGAAAG TGGAGCAGAC CCAAGCAGAT GAAAAGAAGT TCTACTTCGG CGGCTCACCC 
ATCACTCCCC AGTATGCTAA TTTCACCGGA TGTATAAGTA ATGCCTACTT TACCAGGTTG 
GATAGAGATG TAGAGGTCGA AGATTTCCAG CGGTATTCTG AAAAGGTCCA CACTTCTCTT 
TATGAGTGTC CCATTGAGTC TTCACCATTG TTTCTCCTTC ACAAAAAAGG AAGAAATTCC 
TCAAAGCCTA AAACAAATCA GAATAAAAAG GGAGAGAAAA GTAAAGATGC TCCTTCGTGG 
GATCCTGTTG GCCTGAAATT CCTAGAGAGG AATATTCCAA GAGACTCACA CTGCCAGCTT 
TCCAACAGCC CTAGAGCGAT AGAGCACGCC TATCAGTATG GAGGGACAGC CAACAGTCGT 
CAAGAGTTTG AACACCTAAA AAGAGATTTT GGTGAAAAAT CCCAGTTTTC CATTCGTCTG 
AAAACACGTT CCTCCCATGG GATGATTTTC TATGTCTCAG ATCAAGAAGA GAATAACTTC 
ATGACTCTGT TCTTAGCCCA TGGCCGCTTG GTTTTCATGT TTAATGTTGG CCACAAGAAA 
CTGAAGATTA GAAGCCAGGA GAAATACAAT GATGGACTGT GGCATGATGT GATATTTATT 
CGGGAAAAGA GCAGCGGCCG AATGATAATT GATGGGCTTC GAGTCTTAGA AGAAAGTCTT 
CCCCCTACAG GAGCTGACTG GAAAATCAGG GGTCCTATTT ATTTGGGAGG TGTGGCTCCT 
GGAAGGGCTG TGAAAAATGT CCAGATAAAC TCAGTCTACA GCTTCAGTGG CTGCCTCAGC 
AATCTTCAGC TCAATGGGGC CTCCATCACC TCTGCTTCTC AGACATTTAG TGTGACTCCT 
TGTTTCGAAG GTCCAATGGA AACAGGAACA TACTTCTCAA CAGAAGGAGG TTACGTGGTC 
CTAGATGAGT CTTTCAATAT TGGATTGAAG TTTGAGATTG CATTTGAAGT CCGTCCCAGA 
AGCAGTTCCG GAACCCTTGT TCACGGCCAC AGTGTCAATG GAGAATACCT AAATGTTCAC 
ATGAAAAATG GGCAGGTCAT AGTGAAAGTC AACAATGGCA TCAGAGACTT TTCCACCTCA 
GTGACACCCA AGCAGAGTCT CTGTGATGGC AGATGGCACA GAATTACAGT TATTAGAGAT 
TCAAATGTGG TTCAGTTGGA TGTAGACTCT GAAGTGAACC ACGTGGTTGG ACCCCTAAAT 
CCAAAACCAG TTGATCACAG GGAGCCTGTG TTTGTTGGAG GTGTTCCAGA GTCTCTACTG 
ACACCACGCT TGGCCCCCGG CAGACCCTTC ACAGGCTGCA TCCGTCACTT TGTGATTGAC 
GGGCGCCCGG TGAGCTTCAG TAAAGCAGCC CTGGTCAGCG GCGCCGTGAG CATCAACTCC 
TGTCCAGCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192894.1
CDS	457..5931
Translation	MALSSAWCAVLPLWLLCGAACSRAASGDGSAFPFDIEGSSAFGRLDPPETSEPRVAPGSLPPAPKKCDAGFFFAYSEECLPCDCNGNSNECLDGSGLCVHCQRNTTGEHCEKCLDGYIGDAIRGPPRFCQPCPCPLPHVANFAESCYRKNGAVRCICKENYAGPNCERCAPGYYGNPLLIGSTCKKCDCSGNSDPNLIFEDCDEVTGQCRNCLRNTTGFKCERCAPGYYGDARRAKNCAACNCGGGPCDSITGECLEEGFESPTGTDCPTISCDKCIWDLTDDLRLAALSIEESKSGLLSVSSGAAAHRHVNEINSTIYLLKTKLSERENQYVLRKIQINNAENTMKSLQSDMEELAERESQASRKVQLAQKESMDTINHATQLAEQAHNMRDKIQEISSKMLYYGEEQELSSEEISEKLVLAQKMLEEIRRRQPFLTQRELVDEEADEAHELLSQAESWQRQYNDTRSLFPVVLEQLDDYNAKLSDLQESLDQALNHVRDAEDMNRATAARQRDHEKQHERVREQTEGVNASLRTSSNSLMMPRLTLSELDSIIKNASGIYAEIDGAKNELQRKLSNLSNLSHDLVQEAVDHARNLQQEADELSRNLHSSDMNGLVQKALDASNVYENIANYVSEANETAEQALNITDRIYDAVSGIDTQIIYHKDESENLLNQARELQARADSSSDEAVVDTSRRVGGALARKSALRDRLNDAMKRLQAAERGDAQQRLGQSQLITAEANKTTMEVQEATAPMASNLTNWSQNLQSFDSSAYNTAVDSARDAVGNLTEVVPQLLDQLRTVEQKRPASNVSASIQRIRELIAQTRSVASKIQVSMMFDGQSAVEVHPRASMDDLKTFTSLSLYMKPPPVKQSELAVTADQFILYLGSKNAKKEYMGLAIKNDNLVYVYNLGTKDVEIPLDSKPVSTWPAYFSIVKIERVGKHGKVFLTVPSLSSTAEEKFIKKGEFAGDDSLLDLDPEDTVFYVGGVPSNFKLPASLNLPGFVGCLELATLNNDVISLYNFKHIYNMDPSKSVPCARDKLAFTQSRAASYFFDGSSYAIVRDITRRGKFGQVTRFDIEVRTPADNGLVLLMVNGSMFFSLEMRSGYLHVFYDFGFSNGPVHLEDTLKKAQINDAKYHEISIIYHNDKKMILVVDRRHVKSMDNEKMKIPFTDIYIGGAPPEILQSRTLRAHLPLDINFRGCMKGFQFQKKDFNLLEQTETLGVGYGCPEDSLISRRAYFNGQSYIASSQKISFFDGFEGGFNFRTLQPNGLLFYYASGSDMFSISLNNGTVIMDVKGIKVQSADKQYNDGLSHFIITSVSPARYELIVDKSRLGSKNPTKGKVEQTQADEKKFYFGGSPITPQYANFTGCISNAYFTRLDRDVEVEDFQRYSEKVHTSLYECPIESSPLFLLHKKGRNSSKPKTNQNKKGEKSKDAPSWDPVGLKFLERNIPRDSHCQLSNSPRAIEHAYQYGGTANSRQEFEHLKRDFGEKSQFSIRLKTRSSHGMIFYVSDQEENNFMTLFLAHGRLVFMFNVGHKKLKIRSQEKYNDGLWHDVIFIREKSSGRMIIDGLRVLEESLPPTGADWKIRGPIYLGGVAPGRAVKNVQINSVYSFSGCLSNLQLNGASITSASQTFSVTPCFEGPMETGTYFSTEGGYVVLDESFNIGLKFEIAFEVRPRSSSGTLVHGHSVNGEYLNVHMKNGQVIVKVNNGIRDFSTSVTPKQSLCDGRWHRITVIRDSNVVQLDVDSEVNHVVGPLNPKPVDHREPVFVGGVPESLLTPRLAPGRPFTGCIRHFVIDGRPVSFSKAALVSGAVSINSCPAA

Target ORF information:

RefSeq Version	NM_001205965.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit alpha 4 (LAMA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205965.1

ORF Insert Sequence:

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCACGGA CTGCCCAACC ATAAGCTGCG ATAAATGCAT CTGGGATCTG 
ACTGATGACC TTCGGTTAGC AGCGCTCTCA ATTGAAGAAA GCAAATCCGG CCTGCTGAGT 
GTGTCATCTG GTGCCGCAGC TCATAGGCAC GTGAACGAAA TCAACTCCAC CATCTACCTC 
CTCAAAACAA AATTGTCAGA AAGAGAAAAC CAGTATGTCC TAAGAAAGAT ACAAATCAAC 
AATGCTGAAA ACACAATGAA AAGCCTTCAG TCTGACATGG AGGAATTAGC TGAAAGGGAA 
AGCCAAGCTT CAAGAAAGGT CCAGTTGGCT CAGAAGGAAA GCATGGATAC CATTAACCAT 
GCAACTCAGC TTGCAGAGCA AGCCCACAAT ATGAGGGATA AAATCCAAGA GATCAGCAGC 
AAGATGCTGT ATTATGGGGA AGAGCAGGAA CTTAGCTCTG AGGAAATCTC CGAGAAGCTG 
GTGTTGGCAC AGAAGATGCT TGAGGAGATC CGACGCCGTC AGCCGTTCCT CACCCAACGG 
GAACTTGTGG ACGAGGAGGC AGACGAAGCC CATGAATTGC TGAGCCAGGC TGAGAGCTGG 
CAGAGGCAGT ACAATGACAC TCGCTCTCTG TTTCCTGTGG TCCTGGAGCA GCTGGACGAC 
TATAACGCCA AGCTGTCAGA CCTCCAGGAA TCACTCGACC AGGCCCTTAA CCATGTCAGG 
GATGCTGAAG ACATGAACAG AGCCACAGCA GCCAGGCAGC GGGACCACGA GAAACAGCAT 
GAGAGAGTAA GGGAACAGAC GGAAGGGGTG AACGCGTCTC TGAGGACGTC TTCCAACTCA 
CTGATGATGC CACGACTGAC CCTGTCAGAA CTTGATAGCA TAATAAAGAA TGCATCAGGG 
ATTTATGCCG AAATAGATGG AGCCAAAAAT GAACTTCAAA GAAAACTATC CAACCTGAGC 
AACCTCAGTC ATGATTTAGT TCAGGAAGCT GTTGACCATG CACGGAACCT TCAGCAAGAG 
GCTGATGAGT TGAGCAGGAA TTTGCACAGT TCAGATATGA ATGGACTGGT ACAGAAGGCT 
TTGGATGCTT CAAATGTCTA TGAGAATATT GCCAATTACG TTAGTGAAGC CAATGAAACA 
GCAGAACAGG CTTTGAACAT CACTGATCGA ATTTATGATG CTGTGAGTGG GATTGATACA 
CAGATCATTT ACCATAAAGA TGAGAGCGAG AACCTCCTCA ATCAAGCCAG AGAGCTGCAA 
GCAAGGGCAG ATTCTAGCAG TGATGAGGCA GTGGTGGATA CCAGCAGGCG CGTGGGAGGA 
GCCCTAGCAA GGAAGAGTGC TCTCAGAGAC AGATTAAATG ATGCCATGAA AAGACTGCAG 
GCCGCAGAGA GAGGTGATGC CCAGCAGCGC CTGGGTCAGT CCCAGCTGAT CACCGCGGAA 
GCTAACAAGA CCACAATGGA AGTCCAGGAG GCAACCGCCC CGATGGCTAG CAACCTAACC 
AACTGGTCAC AGAATCTCCA AAGTTTTGAC TCTTCTGCTT ACAACACTGC CGTGGACTCT 
GCCAGAGATG CAGTTGGAAA CCTGACAGAG GTTGTCCCTC AGCTCCTGGA TCAGCTTCGT 
ACGGTAGAAC AGAAGCGGCC TGCAAGCAAT GTTTCCGCCA GCATCCAAAG GATCCGAGAG 
CTCATTGCTC AGACCAGAAG TGTTGCCAGC AAGATCCAAG TCTCCATGAT GTTTGATGGC 
CAGTCAGCAG TCGAGGTGCA CCCCAGAGCC AGCATGGATG ACTTAAAGAC CTTTACATCT 
CTGAGCCTGT ACATGAAGCC TCCTCCTGTG AAGCAGTCGG AACTGGCTGT GACTGCAGAT 
CAGTTCATCC TGTACCTCGG AAGCAAAAAC GCCAAAAAAG AGTACATGGG TCTTGCAATC 
AAAAATGACA ACCTGGTGTA CGTTTATAAT TTGGGAACTA AGGATGTAGA GATTCCCTTG 
GACTCGAAGC CTGTCAGTAC CTGGCCTGCT TACTTCAGCA TTGTCAAGAT TGAAAGGGTA 
GGAAAACATG GAAAGGTGTT TTTAACAGTC CCAAGTCTAA GTAGCACTGC AGAGGAAAAG 
TTTATTAAAA AGGGAGAATT TGCTGGAGAT GACTCCTTGT TGGATCTGGA TCCCGAAGAT 
ACTGTGTTTT ATGTTGGGGG CGTGCCATCA AACTTCAAGC TCCCTGCTAG CTTAAACCTG 
CCTGGCTTTG TTGGCTGCCT GGAATTGGCC ACTTTGAATA ATGATGTGAT CAGCTTGTAC 
AACTTTAAGC ACATCTATAA TATGGACCCC TCCAAATCAG TGCCCTGTGC CAGAGACAAA 
CTGGCCTTTA CGCAGAGTCG GGCTGCCAGC TATTTCTTCG ATGGCTCTAG TTATGCCATA 
GTGAGGGATA TCACAAGGAG AGGGAAGTTT GGTCAGGTGA CTCGCTTTGA CATCGAAGTC 
AGAACACCAG CTGACAATGG CCTCGTGCTC CTCATGGTCA ATGGAAGTAT GTTTTTCAGC 
TTGGAAATGC GCAGTGGTTA CCTACATGTG TTCTATGACT TTGGATTTAG CAACGGCCCT 
GTGCATCTTG AAGACACGTT AAAGAAAGCT CAAATTAATG ATGCAAAATA CCATGAGATC 
TCAATCATTT ACCACAATGA TAAGAAAATG ATTTTGGTAG TTGACAGACG ACATGTCAAG 
AGCATGGATA ATGAAAAGAT GAAGATACCT TTTACAGACA TATACATAGG TGGAGCTCCC 
CCAGAAATCT TACAATCAAG GACCTTAAGA GCACATCTTC CCCTAGATAT CAACTTCAGA 
GGATGCATGA AGGGCTTCCA GTTCCAAAAG AAAGATTTCA ATTTATTAGA ACAGACAGAA 
ACCCTGGGAG TTGGCTATGG ATGCCCAGAA GACTCTCTTA TATCTCGCAG AGCATATTTC 
AATGGACAGA GTTACATTGC TTCAAGTCAG AAAATATCTT TTTTTGATGG CTTTGAAGGA 
GGTTTTAATT TCCGAACATT ACAGCCAAAT GGATTACTGT TCTATTATGC ATCAGGGTCA 
GACATGTTCT CCATCTCACT GAATAATGGC ACCGTAATCA TGGATGTAAA GGGAATCAAG 
GTGCAGTCGG CAGATAAGCA GTACAATGAT GGGCTGTCCC ACTTCATCAT CACCTCCGTC 
TCCCCAGCAA GGTATGAATT GATAGTAGAT AAAAGCAGAC TTGGGAGTAA GAACCCTACC 
AAAGGGAAAG TGGAGCAGAC CCAAGCAGAT GAAAAGAAGT TCTACTTCGG CGGCTCACCC 
ATCACTCCCC AGTATGCTAA TTTCACCGGA TGTATAAGTA ATGCCTACTT TACCAGGTTG 
GATAGAGATG TAGAGGTCGA AGATTTCCAG CGGTATTCTG AAAAGGTCCA CACTTCTCTT 
TATGAGTGTC CCATTGAGTC TTCACCATTG TTTCTCCTTC ACAAAAAAGG AAGAAATTCC 
TCAAAGCCTA AAACAAATCA GAATAAAAAG GGAGAGAAAA GTAAAGATGC TCCTTCGTGG 
GATCCTGTTG GCCTGAAATT CCTAGAGAGG AATATTCCAA GAGACTCACA CTGCCAGCTT 
TCCAACAGCC CTAGAGCGAT AGAGCACGCC TATCAGTATG GAGGGACAGC CAACAGTCGT 
CAAGAGTTTG AACACCTAAA AAGAGATTTT GGTGAAAAAT CCCAGTTTTC CATTCGTCTG 
AAAACACGTT CCTCCCATGG GATGATTTTC TATGTCTCAG ATCAAGAAGA GAATAACTTC 
ATGACTCTGT TCTTAGCCCA TGGCCGCTTG GTTTTCATGT TTAATGTTGG CCACAAGAAA 
CTGAAGATTA GAAGCCAGGA GAAATACAAT GATGGACTGT GGCATGATGT GATATTTATT 
CGGGAAAAGA GCAGCGGCCG AATGATAATT GATGGGCTTC GAGTCTTAGA AGAAAGTCTT 
CCCCCTACAG GAGCTGACTG GAAAATCAGG GGTCCTATTT ATTTGGGAGG TGTGGCTCCT 
GGAAGGGCTG TGAAAAATGT CCAGATAAAC TCAGTCTACA GCTTCAGTGG CTGCCTCAGC 
AATCTTCAGC TCAATGGGGC CTCCATCACC TCTGCTTCTC AGACATTTAG TGTGACTCCT 
TGTTTCGAAG GTCCAATGGA AACAGGAACA TACTTCTCAA CAGAAGGAGG TTACGTGGTC 
CTAGATGAGT CTTTCAATAT TGGATTGAAG TTTGAGATTG CATTTGAAGT CCGTCCCAGA 
AGCAGTTCCG GAACCCTTGT TCACGGCCAC AGTGTCAATG GAGAATACCT AAATGTTCAC 
ATGAAAAATG GGCAGGTCAT AGTGAAAGTC AACAATGGCA TCAGAGACTT TTCCACCTCA 
GTGACACCCA AGCAGAGTCT CTGTGATGGC AGATGGCACA GAATTACAGT TATTAGAGAT 
TCAAATGTGG TTCAGTTGGA TGTAGACTCT GAAGTGAACC ACGTGGTTGG ACCCCTAAAT 
CCAAAACCAG TTGATCACAG GGAGCCTGTG TTTGTTGGAG GTGTTCCAGA GTCTCTACTG 
ACACCACGCT TGGCCCCCGG CAGACCCTTC ACAGGCTGCA TCCGTCACTT TGTGATTGAC 
GGGCGCCCGG TGAGCTTCAG TAAAGCAGCC CTGGTCAGCG GCGCCGTGAG CATCAACTCC 
TGTCCAGCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMA4 ( XM_024996678.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852446.1 Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X1, mRNA.

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CloneID	OBa01954
Clone ID Related Accession (Same CDS sequence)	XM_024996678.1 , XM_024996679.1 , NM_001205965.1
Accession Version	XM_024996678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCACGGA CTGCCCAACC ATAAGCTGCG ATAAATGCAT CTGGGATCTG 
ACTGATGACC TTCGGTTAGC AGCGCTCTCA ATTGAAGAAA GCAAATCCGG CCTGCTGAGT 
GTGTCATCTG GTGCCGCAGC TCATAGGCAC GTGAACGAAA TCAACTCCAC CATCTACCTC 
CTCAAAACAA AATTGTCAGA AAGAGAAAAC CAGTATGTCC TAAGAAAGAT ACAAATCAAC 
AATGCTGAAA ACACAATGAA AAGCCTTCAG TCTGACATGG AGGAATTAGC TGAAAGGGAA 
AGCCAAGCTT CAAGAAAGGT CCAGTTGGCT CAGAAGGAAA GCATGGATAC CATTAACCAT 
GCAACTCAGC TTGCAGAGCA AGCCCACAAT ATGAGGGATA AAATCCAAGA GATCAGCAGC 
AAGATGCTGT ATTATGGGGA AGAGCAGGAA CTTAGCTCTG AGGAAATCTC CGAGAAGCTG 
GTGTTGGCAC AGAAGATGCT TGAGGAGATC CGACGCCGTC AGCCGTTCCT CACCCAACGG 
GAACTTGTGG ACGAGGAGGC AGACGAAGCC CATGAATTGC TGAGCCAGGC TGAGAGCTGG 
CAGAGGCAGT ACAATGACAC TCGCTCTCTG TTTCCTGTGG TCCTGGAGCA GCTGGACGAC 
TATAACGCCA AGCTGTCAGA CCTCCAGGAA TCACTCGACC AGGCCCTTAA CCATGTCAGG 
GATGCTGAAG ACATGAACAG AGCCACAGCA GCCAGGCAGC GGGACCACGA GAAACAGCAT 
GAGAGAGTAA GGGAACAGAC GGAAGGGGTG AACGCGTCTC TGAGGACGTC TTCCAACTCA 
CTGATGATGC CACGACTGAC CCTGTCAGAA CTTGATAGCA TAATAAAGAA TGCATCAGGG 
ATTTATGCCG AAATAGATGG AGCCAAAAAT GAACTTCAAA GAAAACTATC CAACCTGAGC 
AACCTCAGTC ATGATTTAGT TCAGGAAGCT GTTGACCATG CACGGAACCT TCAGCAAGAG 
GCTGATGAGT TGAGCAGGAA TTTGCACAGT TCAGATATGA ATGGACTGGT ACAGAAGGCT 
TTGGATGCTT CAAATGTCTA TGAGAATATT GCCAATTACG TTAGTGAAGC CAATGAAACA 
GCAGAACAGG CTTTGAACAT CACTGATCGA ATTTATGATG CTGTGAGTGG GATTGATACA 
CAGATCATTT ACCATAAAGA TGAGAGCGAG AACCTCCTCA ATCAAGCCAG AGAGCTGCAA 
GCAAGGGCAG ATTCTAGCAG TGATGAGGCA GTGGTGGATA CCAGCAGGCG CGTGGGAGGA 
GCCCTAGCAA GGAAGAGTGC TCTCAGAGAC AGATTAAATG ATGCCATGAA AAGACTGCAG 
GCCGCAGAGA GAGGTGATGC CCAGCAGCGC CTGGGTCAGT CCCAGCTGAT CACCGCGGAA 
GCTAACAAGA CCACAATGGA AGTCCAGGAG GCAACCGCCC CGATGGCTAG CAACCTAACC 
AACTGGTCAC AGAATCTCCA AAGTTTTGAC TCTTCTGCTT ACAACACTGC CGTGGACTCT 
GCCAGAGATG CAGTTGGAAA CCTGACAGAG GTTGTCCCTC AGCTCCTGGA TCAGCTTCGT 
ACGGTAGAAC AGAAGCGGCC TGCAAGCAAT GTTTCCGCCA GCATCCAAAG GATCCGAGAG 
CTCATTGCTC AGACCAGAAG TGTTGCCAGC AAGATCCAAG TCTCCATGAT GTTTGATGGC 
CAGTCAGCAG TCGAGGTGCA CCCCAGAGCC AGCATGGATG ACTTAAAGAC CTTTACATCT 
CTGAGCCTGT ACATGAAGCC TCCTCCTGTG AAGCAGTCGG AACTGGCTGT GACTGCAGAT 
CAGTTCATCC TGTACCTCGG AAGCAAAAAC GCCAAAAAAG AGTACATGGG TCTTGCAATC 
AAAAATGACA ACCTGGTGTA CGTTTATAAT TTGGGAACTA AGGATGTAGA GATTCCCTTG 
GACTCGAAGC CTGTCAGTAC CTGGCCTGCT TACTTCAGCA TTGTCAAGAT TGAAAGGGTA 
GGAAAACATG GAAAGGTGTT TTTAACAGTC CCAAGTCTAA GTAGCACTGC AGAGGAAAAG 
TTTATTAAAA AGGGAGAATT TGCTGGAGAT GACTCCTTGT TGGATCTGGA TCCCGAAGAT 
ACTGTGTTTT ATGTTGGGGG CGTGCCATCA AACTTCAAGC TCCCTGCTAG CTTAAACCTG 
CCTGGCTTTG TTGGCTGCCT GGAATTGGCC ACTTTGAATA ATGATGTGAT CAGCTTGTAC 
AACTTTAAGC ACATCTATAA TATGGACCCC TCCAAATCAG TGCCCTGTGC CAGAGACAAA 
CTGGCCTTTA CGCAGAGTCG GGCTGCCAGC TATTTCTTCG ATGGCTCTAG TTATGCCATA 
GTGAGGGATA TCACAAGGAG AGGGAAGTTT GGTCAGGTGA CTCGCTTTGA CATCGAAGTC 
AGAACACCAG CTGACAATGG CCTCGTGCTC CTCATGGTCA ATGGAAGTAT GTTTTTCAGC 
TTGGAAATGC GCAGTGGTTA CCTACATGTG TTCTATGACT TTGGATTTAG CAACGGCCCT 
GTGCATCTTG AAGACACGTT AAAGAAAGCT CAAATTAATG ATGCAAAATA CCATGAGATC 
TCAATCATTT ACCACAATGA TAAGAAAATG ATTTTGGTAG TTGACAGACG ACATGTCAAG 
AGCATGGATA ATGAAAAGAT GAAGATACCT TTTACAGACA TATACATAGG TGGAGCTCCC 
CCAGAAATCT TACAATCAAG GACCTTAAGA GCACATCTTC CCCTAGATAT CAACTTCAGA 
GGATGCATGA AGGGCTTCCA GTTCCAAAAG AAAGATTTCA ATTTATTAGA ACAGACAGAA 
ACCCTGGGAG TTGGCTATGG ATGCCCAGAA GACTCTCTTA TATCTCGCAG AGCATATTTC 
AATGGACAGA GTTACATTGC TTCAAGTCAG AAAATATCTT TTTTTGATGG CTTTGAAGGA 
GGTTTTAATT TCCGAACATT ACAGCCAAAT GGATTACTGT TCTATTATGC ATCAGGGTCA 
GACATGTTCT CCATCTCACT GAATAATGGC ACCGTAATCA TGGATGTAAA GGGAATCAAG 
GTGCAGTCGG CAGATAAGCA GTACAATGAT GGGCTGTCCC ACTTCATCAT CACCTCCGTC 
TCCCCAGCAA GGTATGAATT GATAGTAGAT AAAAGCAGAC TTGGGAGTAA GAACCCTACC 
AAAGGGAAAG TGGAGCAGAC CCAAGCAGAT GAAAAGAAGT TCTACTTCGG CGGCTCACCC 
ATCACTCCCC AGTATGCTAA TTTCACCGGA TGTATAAGTA ATGCCTACTT TACCAGGTTG 
GATAGAGATG TAGAGGTCGA AGATTTCCAG CGGTATTCTG AAAAGGTCCA CACTTCTCTT 
TATGAGTGTC CCATTGAGTC TTCACCATTG TTTCTCCTTC ACAAAAAAGG AAGAAATTCC 
TCAAAGCCTA AAACAAATCA GAATAAAAAG GGAGAGAAAA GTAAAGATGC TCCTTCGTGG 
GATCCTGTTG GCCTGAAATT CCTAGAGAGG AATATTCCAA GAGACTCACA CTGCCAGCTT 
TCCAACAGCC CTAGAGCGAT AGAGCACGCC TATCAGTATG GAGGGACAGC CAACAGTCGT 
CAAGAGTTTG AACACCTAAA AAGAGATTTT GGTGAAAAAT CCCAGTTTTC CATTCGTCTG 
AAAACACGTT CCTCCCATGG GATGATTTTC TATGTCTCAG ATCAAGAAGA GAATAACTTC 
ATGACTCTGT TCTTAGCCCA TGGCCGCTTG GTTTTCATGT TTAATGTTGG CCACAAGAAA 
CTGAAGATTA GAAGCCAGGA GAAATACAAT GATGGACTGT GGCATGATGT GATATTTATT 
CGGGAAAAGA GCAGCGGCCG AATGATAATT GATGGGCTTC GAGTCTTAGA AGAAAGTCTT 
CCCCCTACAG GAGCTGACTG GAAAATCAGG GGTCCTATTT ATTTGGGAGG TGTGGCTCCT 
GGAAGGGCTG TGAAAAATGT CCAGATAAAC TCAGTCTACA GCTTCAGTGG CTGCCTCAGC 
AATCTTCAGC TCAATGGGGC CTCCATCACC TCTGCTTCTC AGACATTTAG TGTGACTCCT 
TGTTTCGAAG GTCCAATGGA AACAGGAACA TACTTCTCAA CAGAAGGAGG TTACGTGGTC 
CTAGATGAGT CTTTCAATAT TGGATTGAAG TTTGAGATTG CATTTGAAGT CCGTCCCAGA 
AGCAGTTCCG GAACCCTTGT TCACGGCCAC AGTGTCAATG GAGAATACCT AAATGTTCAC 
ATGAAAAATG GGCAGGTCAT AGTGAAAGTC AACAATGGCA TCAGAGACTT TTCCACCTCA 
GTGACACCCA AGCAGAGTCT CTGTGATGGC AGATGGCACA GAATTACAGT TATTAGAGAT 
TCAAATGTGG TTCAGTTGGA TGTAGACTCT GAAGTGAACC ACGTGGTTGG ACCCCTAAAT 
CCAAAACCAG TTGATCACAG GGAGCCTGTG TTTGTTGGAG GTGTTCCAGA GTCTCTACTG 
ACACCACGCT TGGCCCCCGG CAGACCCTTC ACAGGCTGCA TCCGTCACTT TGTGATTGAC 
GGGCGCCCGG TGAGCTTCAG TAAAGCAGCC CTGGTCAGCG GCGCCGTGAG CATCAACTCC 
TGTCCAGCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852446.1
CDS	314..5788
Translation	MALSSAWCAVLPLWLLCGAACSRAASGDGSAFPFDIEGSSAFGRLDPPETSEPRVAPGSLPPAPKKCDAGFFFAYSEECLPCDCNGNSNECLDGSGLCVHCQRNTTGEHCEKCLDGYIGDAIRGPPRFCQPCPCPLPHVANFAESCYRKNGAVRCICKENYAGPNCERCAPGYYGNPLLIGSTCKKCDCSGNSDPNLIFEDCDEVTGQCRNCLRNTTGFKCERCAPGYYGDARRAKNCAACNCGGGPCDSITGECLEEGFESPTGTDCPTISCDKCIWDLTDDLRLAALSIEESKSGLLSVSSGAAAHRHVNEINSTIYLLKTKLSERENQYVLRKIQINNAENTMKSLQSDMEELAERESQASRKVQLAQKESMDTINHATQLAEQAHNMRDKIQEISSKMLYYGEEQELSSEEISEKLVLAQKMLEEIRRRQPFLTQRELVDEEADEAHELLSQAESWQRQYNDTRSLFPVVLEQLDDYNAKLSDLQESLDQALNHVRDAEDMNRATAARQRDHEKQHERVREQTEGVNASLRTSSNSLMMPRLTLSELDSIIKNASGIYAEIDGAKNELQRKLSNLSNLSHDLVQEAVDHARNLQQEADELSRNLHSSDMNGLVQKALDASNVYENIANYVSEANETAEQALNITDRIYDAVSGIDTQIIYHKDESENLLNQARELQARADSSSDEAVVDTSRRVGGALARKSALRDRLNDAMKRLQAAERGDAQQRLGQSQLITAEANKTTMEVQEATAPMASNLTNWSQNLQSFDSSAYNTAVDSARDAVGNLTEVVPQLLDQLRTVEQKRPASNVSASIQRIRELIAQTRSVASKIQVSMMFDGQSAVEVHPRASMDDLKTFTSLSLYMKPPPVKQSELAVTADQFILYLGSKNAKKEYMGLAIKNDNLVYVYNLGTKDVEIPLDSKPVSTWPAYFSIVKIERVGKHGKVFLTVPSLSSTAEEKFIKKGEFAGDDSLLDLDPEDTVFYVGGVPSNFKLPASLNLPGFVGCLELATLNNDVISLYNFKHIYNMDPSKSVPCARDKLAFTQSRAASYFFDGSSYAIVRDITRRGKFGQVTRFDIEVRTPADNGLVLLMVNGSMFFSLEMRSGYLHVFYDFGFSNGPVHLEDTLKKAQINDAKYHEISIIYHNDKKMILVVDRRHVKSMDNEKMKIPFTDIYIGGAPPEILQSRTLRAHLPLDINFRGCMKGFQFQKKDFNLLEQTETLGVGYGCPEDSLISRRAYFNGQSYIASSQKISFFDGFEGGFNFRTLQPNGLLFYYASGSDMFSISLNNGTVIMDVKGIKVQSADKQYNDGLSHFIITSVSPARYELIVDKSRLGSKNPTKGKVEQTQADEKKFYFGGSPITPQYANFTGCISNAYFTRLDRDVEVEDFQRYSEKVHTSLYECPIESSPLFLLHKKGRNSSKPKTNQNKKGEKSKDAPSWDPVGLKFLERNIPRDSHCQLSNSPRAIEHAYQYGGTANSRQEFEHLKRDFGEKSQFSIRLKTRSSHGMIFYVSDQEENNFMTLFLAHGRLVFMFNVGHKKLKIRSQEKYNDGLWHDVIFIREKSSGRMIIDGLRVLEESLPPTGADWKIRGPIYLGGVAPGRAVKNVQINSVYSFSGCLSNLQLNGASITSASQTFSVTPCFEGPMETGTYFSTEGGYVVLDESFNIGLKFEIAFEVRPRSSSGTLVHGHSVNGEYLNVHMKNGQVIVKVNNGIRDFSTSVTPKQSLCDGRWHRITVIRDSNVVQLDVDSEVNHVVGPLNPKPVDHREPVFVGGVPESLLTPRLAPGRPFTGCIRHFVIDGRPVSFSKAALVSGAVSINSCPAA

Target ORF information:

RefSeq Version	XM_024996678.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996678.1

ORF Insert Sequence:

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCACGGA CTGCCCAACC ATAAGCTGCG ATAAATGCAT CTGGGATCTG 
ACTGATGACC TTCGGTTAGC AGCGCTCTCA ATTGAAGAAA GCAAATCCGG CCTGCTGAGT 
GTGTCATCTG GTGCCGCAGC TCATAGGCAC GTGAACGAAA TCAACTCCAC CATCTACCTC 
CTCAAAACAA AATTGTCAGA AAGAGAAAAC CAGTATGTCC TAAGAAAGAT ACAAATCAAC 
AATGCTGAAA ACACAATGAA AAGCCTTCAG TCTGACATGG AGGAATTAGC TGAAAGGGAA 
AGCCAAGCTT CAAGAAAGGT CCAGTTGGCT CAGAAGGAAA GCATGGATAC CATTAACCAT 
GCAACTCAGC TTGCAGAGCA AGCCCACAAT ATGAGGGATA AAATCCAAGA GATCAGCAGC 
AAGATGCTGT ATTATGGGGA AGAGCAGGAA CTTAGCTCTG AGGAAATCTC CGAGAAGCTG 
GTGTTGGCAC AGAAGATGCT TGAGGAGATC CGACGCCGTC AGCCGTTCCT CACCCAACGG 
GAACTTGTGG ACGAGGAGGC AGACGAAGCC CATGAATTGC TGAGCCAGGC TGAGAGCTGG 
CAGAGGCAGT ACAATGACAC TCGCTCTCTG TTTCCTGTGG TCCTGGAGCA GCTGGACGAC 
TATAACGCCA AGCTGTCAGA CCTCCAGGAA TCACTCGACC AGGCCCTTAA CCATGTCAGG 
GATGCTGAAG ACATGAACAG AGCCACAGCA GCCAGGCAGC GGGACCACGA GAAACAGCAT 
GAGAGAGTAA GGGAACAGAC GGAAGGGGTG AACGCGTCTC TGAGGACGTC TTCCAACTCA 
CTGATGATGC CACGACTGAC CCTGTCAGAA CTTGATAGCA TAATAAAGAA TGCATCAGGG 
ATTTATGCCG AAATAGATGG AGCCAAAAAT GAACTTCAAA GAAAACTATC CAACCTGAGC 
AACCTCAGTC ATGATTTAGT TCAGGAAGCT GTTGACCATG CACGGAACCT TCAGCAAGAG 
GCTGATGAGT TGAGCAGGAA TTTGCACAGT TCAGATATGA ATGGACTGGT ACAGAAGGCT 
TTGGATGCTT CAAATGTCTA TGAGAATATT GCCAATTACG TTAGTGAAGC CAATGAAACA 
GCAGAACAGG CTTTGAACAT CACTGATCGA ATTTATGATG CTGTGAGTGG GATTGATACA 
CAGATCATTT ACCATAAAGA TGAGAGCGAG AACCTCCTCA ATCAAGCCAG AGAGCTGCAA 
GCAAGGGCAG ATTCTAGCAG TGATGAGGCA GTGGTGGATA CCAGCAGGCG CGTGGGAGGA 
GCCCTAGCAA GGAAGAGTGC TCTCAGAGAC AGATTAAATG ATGCCATGAA AAGACTGCAG 
GCCGCAGAGA GAGGTGATGC CCAGCAGCGC CTGGGTCAGT CCCAGCTGAT CACCGCGGAA 
GCTAACAAGA CCACAATGGA AGTCCAGGAG GCAACCGCCC CGATGGCTAG CAACCTAACC 
AACTGGTCAC AGAATCTCCA AAGTTTTGAC TCTTCTGCTT ACAACACTGC CGTGGACTCT 
GCCAGAGATG CAGTTGGAAA CCTGACAGAG GTTGTCCCTC AGCTCCTGGA TCAGCTTCGT 
ACGGTAGAAC AGAAGCGGCC TGCAAGCAAT GTTTCCGCCA GCATCCAAAG GATCCGAGAG 
CTCATTGCTC AGACCAGAAG TGTTGCCAGC AAGATCCAAG TCTCCATGAT GTTTGATGGC 
CAGTCAGCAG TCGAGGTGCA CCCCAGAGCC AGCATGGATG ACTTAAAGAC CTTTACATCT 
CTGAGCCTGT ACATGAAGCC TCCTCCTGTG AAGCAGTCGG AACTGGCTGT GACTGCAGAT 
CAGTTCATCC TGTACCTCGG AAGCAAAAAC GCCAAAAAAG AGTACATGGG TCTTGCAATC 
AAAAATGACA ACCTGGTGTA CGTTTATAAT TTGGGAACTA AGGATGTAGA GATTCCCTTG 
GACTCGAAGC CTGTCAGTAC CTGGCCTGCT TACTTCAGCA TTGTCAAGAT TGAAAGGGTA 
GGAAAACATG GAAAGGTGTT TTTAACAGTC CCAAGTCTAA GTAGCACTGC AGAGGAAAAG 
TTTATTAAAA AGGGAGAATT TGCTGGAGAT GACTCCTTGT TGGATCTGGA TCCCGAAGAT 
ACTGTGTTTT ATGTTGGGGG CGTGCCATCA AACTTCAAGC TCCCTGCTAG CTTAAACCTG 
CCTGGCTTTG TTGGCTGCCT GGAATTGGCC ACTTTGAATA ATGATGTGAT CAGCTTGTAC 
AACTTTAAGC ACATCTATAA TATGGACCCC TCCAAATCAG TGCCCTGTGC CAGAGACAAA 
CTGGCCTTTA CGCAGAGTCG GGCTGCCAGC TATTTCTTCG ATGGCTCTAG TTATGCCATA 
GTGAGGGATA TCACAAGGAG AGGGAAGTTT GGTCAGGTGA CTCGCTTTGA CATCGAAGTC 
AGAACACCAG CTGACAATGG CCTCGTGCTC CTCATGGTCA ATGGAAGTAT GTTTTTCAGC 
TTGGAAATGC GCAGTGGTTA CCTACATGTG TTCTATGACT TTGGATTTAG CAACGGCCCT 
GTGCATCTTG AAGACACGTT AAAGAAAGCT CAAATTAATG ATGCAAAATA CCATGAGATC 
TCAATCATTT ACCACAATGA TAAGAAAATG ATTTTGGTAG TTGACAGACG ACATGTCAAG 
AGCATGGATA ATGAAAAGAT GAAGATACCT TTTACAGACA TATACATAGG TGGAGCTCCC 
CCAGAAATCT TACAATCAAG GACCTTAAGA GCACATCTTC CCCTAGATAT CAACTTCAGA 
GGATGCATGA AGGGCTTCCA GTTCCAAAAG AAAGATTTCA ATTTATTAGA ACAGACAGAA 
ACCCTGGGAG TTGGCTATGG ATGCCCAGAA GACTCTCTTA TATCTCGCAG AGCATATTTC 
AATGGACAGA GTTACATTGC TTCAAGTCAG AAAATATCTT TTTTTGATGG CTTTGAAGGA 
GGTTTTAATT TCCGAACATT ACAGCCAAAT GGATTACTGT TCTATTATGC ATCAGGGTCA 
GACATGTTCT CCATCTCACT GAATAATGGC ACCGTAATCA TGGATGTAAA GGGAATCAAG 
GTGCAGTCGG CAGATAAGCA GTACAATGAT GGGCTGTCCC ACTTCATCAT CACCTCCGTC 
TCCCCAGCAA GGTATGAATT GATAGTAGAT AAAAGCAGAC TTGGGAGTAA GAACCCTACC 
AAAGGGAAAG TGGAGCAGAC CCAAGCAGAT GAAAAGAAGT TCTACTTCGG CGGCTCACCC 
ATCACTCCCC AGTATGCTAA TTTCACCGGA TGTATAAGTA ATGCCTACTT TACCAGGTTG 
GATAGAGATG TAGAGGTCGA AGATTTCCAG CGGTATTCTG AAAAGGTCCA CACTTCTCTT 
TATGAGTGTC CCATTGAGTC TTCACCATTG TTTCTCCTTC ACAAAAAAGG AAGAAATTCC 
TCAAAGCCTA AAACAAATCA GAATAAAAAG GGAGAGAAAA GTAAAGATGC TCCTTCGTGG 
GATCCTGTTG GCCTGAAATT CCTAGAGAGG AATATTCCAA GAGACTCACA CTGCCAGCTT 
TCCAACAGCC CTAGAGCGAT AGAGCACGCC TATCAGTATG GAGGGACAGC CAACAGTCGT 
CAAGAGTTTG AACACCTAAA AAGAGATTTT GGTGAAAAAT CCCAGTTTTC CATTCGTCTG 
AAAACACGTT CCTCCCATGG GATGATTTTC TATGTCTCAG ATCAAGAAGA GAATAACTTC 
ATGACTCTGT TCTTAGCCCA TGGCCGCTTG GTTTTCATGT TTAATGTTGG CCACAAGAAA 
CTGAAGATTA GAAGCCAGGA GAAATACAAT GATGGACTGT GGCATGATGT GATATTTATT 
CGGGAAAAGA GCAGCGGCCG AATGATAATT GATGGGCTTC GAGTCTTAGA AGAAAGTCTT 
CCCCCTACAG GAGCTGACTG GAAAATCAGG GGTCCTATTT ATTTGGGAGG TGTGGCTCCT 
GGAAGGGCTG TGAAAAATGT CCAGATAAAC TCAGTCTACA GCTTCAGTGG CTGCCTCAGC 
AATCTTCAGC TCAATGGGGC CTCCATCACC TCTGCTTCTC AGACATTTAG TGTGACTCCT 
TGTTTCGAAG GTCCAATGGA AACAGGAACA TACTTCTCAA CAGAAGGAGG TTACGTGGTC 
CTAGATGAGT CTTTCAATAT TGGATTGAAG TTTGAGATTG CATTTGAAGT CCGTCCCAGA 
AGCAGTTCCG GAACCCTTGT TCACGGCCAC AGTGTCAATG GAGAATACCT AAATGTTCAC 
ATGAAAAATG GGCAGGTCAT AGTGAAAGTC AACAATGGCA TCAGAGACTT TTCCACCTCA 
GTGACACCCA AGCAGAGTCT CTGTGATGGC AGATGGCACA GAATTACAGT TATTAGAGAT 
TCAAATGTGG TTCAGTTGGA TGTAGACTCT GAAGTGAACC ACGTGGTTGG ACCCCTAAAT 
CCAAAACCAG TTGATCACAG GGAGCCTGTG TTTGTTGGAG GTGTTCCAGA GTCTCTACTG 
ACACCACGCT TGGCCCCCGG CAGACCCTTC ACAGGCTGCA TCCGTCACTT TGTGATTGAC 
GGGCGCCCGG TGAGCTTCAG TAAAGCAGCC CTGGTCAGCG GCGCCGTGAG CATCAACTCC 
TGTCCAGCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMA4 ( XM_024996679.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852447.1 Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X2, mRNA.

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CloneID	OBa01954
Clone ID Related Accession (Same CDS sequence)	XM_024996678.1 , XM_024996679.1 , NM_001205965.1
Accession Version	XM_024996679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCACGGA CTGCCCAACC ATAAGCTGCG ATAAATGCAT CTGGGATCTG 
ACTGATGACC TTCGGTTAGC AGCGCTCTCA ATTGAAGAAA GCAAATCCGG CCTGCTGAGT 
GTGTCATCTG GTGCCGCAGC TCATAGGCAC GTGAACGAAA TCAACTCCAC CATCTACCTC 
CTCAAAACAA AATTGTCAGA AAGAGAAAAC CAGTATGTCC TAAGAAAGAT ACAAATCAAC 
AATGCTGAAA ACACAATGAA AAGCCTTCAG TCTGACATGG AGGAATTAGC TGAAAGGGAA 
AGCCAAGCTT CAAGAAAGGT CCAGTTGGCT CAGAAGGAAA GCATGGATAC CATTAACCAT 
GCAACTCAGC TTGCAGAGCA AGCCCACAAT ATGAGGGATA AAATCCAAGA GATCAGCAGC 
AAGATGCTGT ATTATGGGGA AGAGCAGGAA CTTAGCTCTG AGGAAATCTC CGAGAAGCTG 
GTGTTGGCAC AGAAGATGCT TGAGGAGATC CGACGCCGTC AGCCGTTCCT CACCCAACGG 
GAACTTGTGG ACGAGGAGGC AGACGAAGCC CATGAATTGC TGAGCCAGGC TGAGAGCTGG 
CAGAGGCAGT ACAATGACAC TCGCTCTCTG TTTCCTGTGG TCCTGGAGCA GCTGGACGAC 
TATAACGCCA AGCTGTCAGA CCTCCAGGAA TCACTCGACC AGGCCCTTAA CCATGTCAGG 
GATGCTGAAG ACATGAACAG AGCCACAGCA GCCAGGCAGC GGGACCACGA GAAACAGCAT 
GAGAGAGTAA GGGAACAGAC GGAAGGGGTG AACGCGTCTC TGAGGACGTC TTCCAACTCA 
CTGATGATGC CACGACTGAC CCTGTCAGAA CTTGATAGCA TAATAAAGAA TGCATCAGGG 
ATTTATGCCG AAATAGATGG AGCCAAAAAT GAACTTCAAA GAAAACTATC CAACCTGAGC 
AACCTCAGTC ATGATTTAGT TCAGGAAGCT GTTGACCATG CACGGAACCT TCAGCAAGAG 
GCTGATGAGT TGAGCAGGAA TTTGCACAGT TCAGATATGA ATGGACTGGT ACAGAAGGCT 
TTGGATGCTT CAAATGTCTA TGAGAATATT GCCAATTACG TTAGTGAAGC CAATGAAACA 
GCAGAACAGG CTTTGAACAT CACTGATCGA ATTTATGATG CTGTGAGTGG GATTGATACA 
CAGATCATTT ACCATAAAGA TGAGAGCGAG AACCTCCTCA ATCAAGCCAG AGAGCTGCAA 
GCAAGGGCAG ATTCTAGCAG TGATGAGGCA GTGGTGGATA CCAGCAGGCG CGTGGGAGGA 
GCCCTAGCAA GGAAGAGTGC TCTCAGAGAC AGATTAAATG ATGCCATGAA AAGACTGCAG 
GCCGCAGAGA GAGGTGATGC CCAGCAGCGC CTGGGTCAGT CCCAGCTGAT CACCGCGGAA 
GCTAACAAGA CCACAATGGA AGTCCAGGAG GCAACCGCCC CGATGGCTAG CAACCTAACC 
AACTGGTCAC AGAATCTCCA AAGTTTTGAC TCTTCTGCTT ACAACACTGC CGTGGACTCT 
GCCAGAGATG CAGTTGGAAA CCTGACAGAG GTTGTCCCTC AGCTCCTGGA TCAGCTTCGT 
ACGGTAGAAC AGAAGCGGCC TGCAAGCAAT GTTTCCGCCA GCATCCAAAG GATCCGAGAG 
CTCATTGCTC AGACCAGAAG TGTTGCCAGC AAGATCCAAG TCTCCATGAT GTTTGATGGC 
CAGTCAGCAG TCGAGGTGCA CCCCAGAGCC AGCATGGATG ACTTAAAGAC CTTTACATCT 
CTGAGCCTGT ACATGAAGCC TCCTCCTGTG AAGCAGTCGG AACTGGCTGT GACTGCAGAT 
CAGTTCATCC TGTACCTCGG AAGCAAAAAC GCCAAAAAAG AGTACATGGG TCTTGCAATC 
AAAAATGACA ACCTGGTGTA CGTTTATAAT TTGGGAACTA AGGATGTAGA GATTCCCTTG 
GACTCGAAGC CTGTCAGTAC CTGGCCTGCT TACTTCAGCA TTGTCAAGAT TGAAAGGGTA 
GGAAAACATG GAAAGGTGTT TTTAACAGTC CCAAGTCTAA GTAGCACTGC AGAGGAAAAG 
TTTATTAAAA AGGGAGAATT TGCTGGAGAT GACTCCTTGT TGGATCTGGA TCCCGAAGAT 
ACTGTGTTTT ATGTTGGGGG CGTGCCATCA AACTTCAAGC TCCCTGCTAG CTTAAACCTG 
CCTGGCTTTG TTGGCTGCCT GGAATTGGCC ACTTTGAATA ATGATGTGAT CAGCTTGTAC 
AACTTTAAGC ACATCTATAA TATGGACCCC TCCAAATCAG TGCCCTGTGC CAGAGACAAA 
CTGGCCTTTA CGCAGAGTCG GGCTGCCAGC TATTTCTTCG ATGGCTCTAG TTATGCCATA 
GTGAGGGATA TCACAAGGAG AGGGAAGTTT GGTCAGGTGA CTCGCTTTGA CATCGAAGTC 
AGAACACCAG CTGACAATGG CCTCGTGCTC CTCATGGTCA ATGGAAGTAT GTTTTTCAGC 
TTGGAAATGC GCAGTGGTTA CCTACATGTG TTCTATGACT TTGGATTTAG CAACGGCCCT 
GTGCATCTTG AAGACACGTT AAAGAAAGCT CAAATTAATG ATGCAAAATA CCATGAGATC 
TCAATCATTT ACCACAATGA TAAGAAAATG ATTTTGGTAG TTGACAGACG ACATGTCAAG 
AGCATGGATA ATGAAAAGAT GAAGATACCT TTTACAGACA TATACATAGG TGGAGCTCCC 
CCAGAAATCT TACAATCAAG GACCTTAAGA GCACATCTTC CCCTAGATAT CAACTTCAGA 
GGATGCATGA AGGGCTTCCA GTTCCAAAAG AAAGATTTCA ATTTATTAGA ACAGACAGAA 
ACCCTGGGAG TTGGCTATGG ATGCCCAGAA GACTCTCTTA TATCTCGCAG AGCATATTTC 
AATGGACAGA GTTACATTGC TTCAAGTCAG AAAATATCTT TTTTTGATGG CTTTGAAGGA 
GGTTTTAATT TCCGAACATT ACAGCCAAAT GGATTACTGT TCTATTATGC ATCAGGGTCA 
GACATGTTCT CCATCTCACT GAATAATGGC ACCGTAATCA TGGATGTAAA GGGAATCAAG 
GTGCAGTCGG CAGATAAGCA GTACAATGAT GGGCTGTCCC ACTTCATCAT CACCTCCGTC 
TCCCCAGCAA GGTATGAATT GATAGTAGAT AAAAGCAGAC TTGGGAGTAA GAACCCTACC 
AAAGGGAAAG TGGAGCAGAC CCAAGCAGAT GAAAAGAAGT TCTACTTCGG CGGCTCACCC 
ATCACTCCCC AGTATGCTAA TTTCACCGGA TGTATAAGTA ATGCCTACTT TACCAGGTTG 
GATAGAGATG TAGAGGTCGA AGATTTCCAG CGGTATTCTG AAAAGGTCCA CACTTCTCTT 
TATGAGTGTC CCATTGAGTC TTCACCATTG TTTCTCCTTC ACAAAAAAGG AAGAAATTCC 
TCAAAGCCTA AAACAAATCA GAATAAAAAG GGAGAGAAAA GTAAAGATGC TCCTTCGTGG 
GATCCTGTTG GCCTGAAATT CCTAGAGAGG AATATTCCAA GAGACTCACA CTGCCAGCTT 
TCCAACAGCC CTAGAGCGAT AGAGCACGCC TATCAGTATG GAGGGACAGC CAACAGTCGT 
CAAGAGTTTG AACACCTAAA AAGAGATTTT GGTGAAAAAT CCCAGTTTTC CATTCGTCTG 
AAAACACGTT CCTCCCATGG GATGATTTTC TATGTCTCAG ATCAAGAAGA GAATAACTTC 
ATGACTCTGT TCTTAGCCCA TGGCCGCTTG GTTTTCATGT TTAATGTTGG CCACAAGAAA 
CTGAAGATTA GAAGCCAGGA GAAATACAAT GATGGACTGT GGCATGATGT GATATTTATT 
CGGGAAAAGA GCAGCGGCCG AATGATAATT GATGGGCTTC GAGTCTTAGA AGAAAGTCTT 
CCCCCTACAG GAGCTGACTG GAAAATCAGG GGTCCTATTT ATTTGGGAGG TGTGGCTCCT 
GGAAGGGCTG TGAAAAATGT CCAGATAAAC TCAGTCTACA GCTTCAGTGG CTGCCTCAGC 
AATCTTCAGC TCAATGGGGC CTCCATCACC TCTGCTTCTC AGACATTTAG TGTGACTCCT 
TGTTTCGAAG GTCCAATGGA AACAGGAACA TACTTCTCAA CAGAAGGAGG TTACGTGGTC 
CTAGATGAGT CTTTCAATAT TGGATTGAAG TTTGAGATTG CATTTGAAGT CCGTCCCAGA 
AGCAGTTCCG GAACCCTTGT TCACGGCCAC AGTGTCAATG GAGAATACCT AAATGTTCAC 
ATGAAAAATG GGCAGGTCAT AGTGAAAGTC AACAATGGCA TCAGAGACTT TTCCACCTCA 
GTGACACCCA AGCAGAGTCT CTGTGATGGC AGATGGCACA GAATTACAGT TATTAGAGAT 
TCAAATGTGG TTCAGTTGGA TGTAGACTCT GAAGTGAACC ACGTGGTTGG ACCCCTAAAT 
CCAAAACCAG TTGATCACAG GGAGCCTGTG TTTGTTGGAG GTGTTCCAGA GTCTCTACTG 
ACACCACGCT TGGCCCCCGG CAGACCCTTC ACAGGCTGCA TCCGTCACTT TGTGATTGAC 
GGGCGCCCGG TGAGCTTCAG TAAAGCAGCC CTGGTCAGCG GCGCCGTGAG CATCAACTCC 
TGTCCAGCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852447.1
CDS	310..5784
Translation	MALSSAWCAVLPLWLLCGAACSRAASGDGSAFPFDIEGSSAFGRLDPPETSEPRVAPGSLPPAPKKCDAGFFFAYSEECLPCDCNGNSNECLDGSGLCVHCQRNTTGEHCEKCLDGYIGDAIRGPPRFCQPCPCPLPHVANFAESCYRKNGAVRCICKENYAGPNCERCAPGYYGNPLLIGSTCKKCDCSGNSDPNLIFEDCDEVTGQCRNCLRNTTGFKCERCAPGYYGDARRAKNCAACNCGGGPCDSITGECLEEGFESPTGTDCPTISCDKCIWDLTDDLRLAALSIEESKSGLLSVSSGAAAHRHVNEINSTIYLLKTKLSERENQYVLRKIQINNAENTMKSLQSDMEELAERESQASRKVQLAQKESMDTINHATQLAEQAHNMRDKIQEISSKMLYYGEEQELSSEEISEKLVLAQKMLEEIRRRQPFLTQRELVDEEADEAHELLSQAESWQRQYNDTRSLFPVVLEQLDDYNAKLSDLQESLDQALNHVRDAEDMNRATAARQRDHEKQHERVREQTEGVNASLRTSSNSLMMPRLTLSELDSIIKNASGIYAEIDGAKNELQRKLSNLSNLSHDLVQEAVDHARNLQQEADELSRNLHSSDMNGLVQKALDASNVYENIANYVSEANETAEQALNITDRIYDAVSGIDTQIIYHKDESENLLNQARELQARADSSSDEAVVDTSRRVGGALARKSALRDRLNDAMKRLQAAERGDAQQRLGQSQLITAEANKTTMEVQEATAPMASNLTNWSQNLQSFDSSAYNTAVDSARDAVGNLTEVVPQLLDQLRTVEQKRPASNVSASIQRIRELIAQTRSVASKIQVSMMFDGQSAVEVHPRASMDDLKTFTSLSLYMKPPPVKQSELAVTADQFILYLGSKNAKKEYMGLAIKNDNLVYVYNLGTKDVEIPLDSKPVSTWPAYFSIVKIERVGKHGKVFLTVPSLSSTAEEKFIKKGEFAGDDSLLDLDPEDTVFYVGGVPSNFKLPASLNLPGFVGCLELATLNNDVISLYNFKHIYNMDPSKSVPCARDKLAFTQSRAASYFFDGSSYAIVRDITRRGKFGQVTRFDIEVRTPADNGLVLLMVNGSMFFSLEMRSGYLHVFYDFGFSNGPVHLEDTLKKAQINDAKYHEISIIYHNDKKMILVVDRRHVKSMDNEKMKIPFTDIYIGGAPPEILQSRTLRAHLPLDINFRGCMKGFQFQKKDFNLLEQTETLGVGYGCPEDSLISRRAYFNGQSYIASSQKISFFDGFEGGFNFRTLQPNGLLFYYASGSDMFSISLNNGTVIMDVKGIKVQSADKQYNDGLSHFIITSVSPARYELIVDKSRLGSKNPTKGKVEQTQADEKKFYFGGSPITPQYANFTGCISNAYFTRLDRDVEVEDFQRYSEKVHTSLYECPIESSPLFLLHKKGRNSSKPKTNQNKKGEKSKDAPSWDPVGLKFLERNIPRDSHCQLSNSPRAIEHAYQYGGTANSRQEFEHLKRDFGEKSQFSIRLKTRSSHGMIFYVSDQEENNFMTLFLAHGRLVFMFNVGHKKLKIRSQEKYNDGLWHDVIFIREKSSGRMIIDGLRVLEESLPPTGADWKIRGPIYLGGVAPGRAVKNVQINSVYSFSGCLSNLQLNGASITSASQTFSVTPCFEGPMETGTYFSTEGGYVVLDESFNIGLKFEIAFEVRPRSSSGTLVHGHSVNGEYLNVHMKNGQVIVKVNNGIRDFSTSVTPKQSLCDGRWHRITVIRDSNVVQLDVDSEVNHVVGPLNPKPVDHREPVFVGGVPESLLTPRLAPGRPFTGCIRHFVIDGRPVSFSKAALVSGAVSINSCPAA

Target ORF information:

RefSeq Version	XM_024996679.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit alpha 4 (LAMA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996679.1

ORF Insert Sequence:

ATGGCTTTGA GCTCAGCCTG GTGCGCAGTC CTGCCCCTGT GGCTCCTCTG CGGCGCCGCC 
TGCTCCCGCG CCGCGTCTGG GGACGGCAGC GCCTTCCCTT TTGACATCGA AGGGAGCTCA 
GCGTTCGGCC GACTAGACCC GCCTGAGACC AGCGAGCCCC GCGTGGCTCC CGGAAGCCTG 
CCGCCTGCTC CCAAGAAATG TGACGCTGGA TTCTTCTTTG CCTACTCGGA AGAATGTCTG 
CCCTGTGACT GTAATGGTAA TTCCAACGAG TGCTTGGACG GCTCAGGACT CTGTGTGCAC 
TGCCAGCGGA ACACAACAGG CGAGCACTGT GAGAAATGTC TAGATGGTTA TATTGGAGAT 
GCCATCAGGG GGCCCCCCCG GTTCTGCCAG CCATGCCCCT GCCCCCTGCC TCATGTTGCC 
AATTTTGCAG AGTCCTGCTA TAGGAAAAAC GGAGCTGTTC GGTGTATTTG TAAAGAAAAC 
TATGCTGGAC CTAATTGTGA AAGATGTGCT CCTGGTTACT ATGGAAACCC CTTGCTGATC 
GGAAGCACTT GTAAGAAATG TGACTGCAGT GGGAATTCAG ATCCTAATCT GATCTTTGAA 
GATTGTGATG AGGTCACTGG CCAGTGTAGG AATTGCTTAC GCAACACCAC TGGATTCAAG 
TGTGAACGGT GCGCGCCTGG GTACTATGGA GATGCGAGGA GAGCCAAGAA CTGTGCAGCG 
TGCAATTGTG GGGGAGGCCC ATGTGACAGC ATAACCGGAG AGTGCTTGGA AGAAGGTTTT 
GAGTCCCCTA CAGGCACGGA CTGCCCAACC ATAAGCTGCG ATAAATGCAT CTGGGATCTG 
ACTGATGACC TTCGGTTAGC AGCGCTCTCA ATTGAAGAAA GCAAATCCGG CCTGCTGAGT 
GTGTCATCTG GTGCCGCAGC TCATAGGCAC GTGAACGAAA TCAACTCCAC CATCTACCTC 
CTCAAAACAA AATTGTCAGA AAGAGAAAAC CAGTATGTCC TAAGAAAGAT ACAAATCAAC 
AATGCTGAAA ACACAATGAA AAGCCTTCAG TCTGACATGG AGGAATTAGC TGAAAGGGAA 
AGCCAAGCTT CAAGAAAGGT CCAGTTGGCT CAGAAGGAAA GCATGGATAC CATTAACCAT 
GCAACTCAGC TTGCAGAGCA AGCCCACAAT ATGAGGGATA AAATCCAAGA GATCAGCAGC 
AAGATGCTGT ATTATGGGGA AGAGCAGGAA CTTAGCTCTG AGGAAATCTC CGAGAAGCTG 
GTGTTGGCAC AGAAGATGCT TGAGGAGATC CGACGCCGTC AGCCGTTCCT CACCCAACGG 
GAACTTGTGG ACGAGGAGGC AGACGAAGCC CATGAATTGC TGAGCCAGGC TGAGAGCTGG 
CAGAGGCAGT ACAATGACAC TCGCTCTCTG TTTCCTGTGG TCCTGGAGCA GCTGGACGAC 
TATAACGCCA AGCTGTCAGA CCTCCAGGAA TCACTCGACC AGGCCCTTAA CCATGTCAGG 
GATGCTGAAG ACATGAACAG AGCCACAGCA GCCAGGCAGC GGGACCACGA GAAACAGCAT 
GAGAGAGTAA GGGAACAGAC GGAAGGGGTG AACGCGTCTC TGAGGACGTC TTCCAACTCA 
CTGATGATGC CACGACTGAC CCTGTCAGAA CTTGATAGCA TAATAAAGAA TGCATCAGGG 
ATTTATGCCG AAATAGATGG AGCCAAAAAT GAACTTCAAA GAAAACTATC CAACCTGAGC 
AACCTCAGTC ATGATTTAGT TCAGGAAGCT GTTGACCATG CACGGAACCT TCAGCAAGAG 
GCTGATGAGT TGAGCAGGAA TTTGCACAGT TCAGATATGA ATGGACTGGT ACAGAAGGCT 
TTGGATGCTT CAAATGTCTA TGAGAATATT GCCAATTACG TTAGTGAAGC CAATGAAACA 
GCAGAACAGG CTTTGAACAT CACTGATCGA ATTTATGATG CTGTGAGTGG GATTGATACA 
CAGATCATTT ACCATAAAGA TGAGAGCGAG AACCTCCTCA ATCAAGCCAG AGAGCTGCAA 
GCAAGGGCAG ATTCTAGCAG TGATGAGGCA GTGGTGGATA CCAGCAGGCG CGTGGGAGGA 
GCCCTAGCAA GGAAGAGTGC TCTCAGAGAC AGATTAAATG ATGCCATGAA AAGACTGCAG 
GCCGCAGAGA GAGGTGATGC CCAGCAGCGC CTGGGTCAGT CCCAGCTGAT CACCGCGGAA 
GCTAACAAGA CCACAATGGA AGTCCAGGAG GCAACCGCCC CGATGGCTAG CAACCTAACC 
AACTGGTCAC AGAATCTCCA AAGTTTTGAC TCTTCTGCTT ACAACACTGC CGTGGACTCT 
GCCAGAGATG CAGTTGGAAA CCTGACAGAG GTTGTCCCTC AGCTCCTGGA TCAGCTTCGT 
ACGGTAGAAC AGAAGCGGCC TGCAAGCAAT GTTTCCGCCA GCATCCAAAG GATCCGAGAG 
CTCATTGCTC AGACCAGAAG TGTTGCCAGC AAGATCCAAG TCTCCATGAT GTTTGATGGC 
CAGTCAGCAG TCGAGGTGCA CCCCAGAGCC AGCATGGATG ACTTAAAGAC CTTTACATCT 
CTGAGCCTGT ACATGAAGCC TCCTCCTGTG AAGCAGTCGG AACTGGCTGT GACTGCAGAT 
CAGTTCATCC TGTACCTCGG AAGCAAAAAC GCCAAAAAAG AGTACATGGG TCTTGCAATC 
AAAAATGACA ACCTGGTGTA CGTTTATAAT TTGGGAACTA AGGATGTAGA GATTCCCTTG 
GACTCGAAGC CTGTCAGTAC CTGGCCTGCT TACTTCAGCA TTGTCAAGAT TGAAAGGGTA 
GGAAAACATG GAAAGGTGTT TTTAACAGTC CCAAGTCTAA GTAGCACTGC AGAGGAAAAG 
TTTATTAAAA AGGGAGAATT TGCTGGAGAT GACTCCTTGT TGGATCTGGA TCCCGAAGAT 
ACTGTGTTTT ATGTTGGGGG CGTGCCATCA AACTTCAAGC TCCCTGCTAG CTTAAACCTG 
CCTGGCTTTG TTGGCTGCCT GGAATTGGCC ACTTTGAATA ATGATGTGAT CAGCTTGTAC 
AACTTTAAGC ACATCTATAA TATGGACCCC TCCAAATCAG TGCCCTGTGC CAGAGACAAA 
CTGGCCTTTA CGCAGAGTCG GGCTGCCAGC TATTTCTTCG ATGGCTCTAG TTATGCCATA 
GTGAGGGATA TCACAAGGAG AGGGAAGTTT GGTCAGGTGA CTCGCTTTGA CATCGAAGTC 
AGAACACCAG CTGACAATGG CCTCGTGCTC CTCATGGTCA ATGGAAGTAT GTTTTTCAGC 
TTGGAAATGC GCAGTGGTTA CCTACATGTG TTCTATGACT TTGGATTTAG CAACGGCCCT 
GTGCATCTTG AAGACACGTT AAAGAAAGCT CAAATTAATG ATGCAAAATA CCATGAGATC 
TCAATCATTT ACCACAATGA TAAGAAAATG ATTTTGGTAG TTGACAGACG ACATGTCAAG 
AGCATGGATA ATGAAAAGAT GAAGATACCT TTTACAGACA TATACATAGG TGGAGCTCCC 
CCAGAAATCT TACAATCAAG GACCTTAAGA GCACATCTTC CCCTAGATAT CAACTTCAGA 
GGATGCATGA AGGGCTTCCA GTTCCAAAAG AAAGATTTCA ATTTATTAGA ACAGACAGAA 
ACCCTGGGAG TTGGCTATGG ATGCCCAGAA GACTCTCTTA TATCTCGCAG AGCATATTTC 
AATGGACAGA GTTACATTGC TTCAAGTCAG AAAATATCTT TTTTTGATGG CTTTGAAGGA 
GGTTTTAATT TCCGAACATT ACAGCCAAAT GGATTACTGT TCTATTATGC ATCAGGGTCA 
GACATGTTCT CCATCTCACT GAATAATGGC ACCGTAATCA TGGATGTAAA GGGAATCAAG 
GTGCAGTCGG CAGATAAGCA GTACAATGAT GGGCTGTCCC ACTTCATCAT CACCTCCGTC 
TCCCCAGCAA GGTATGAATT GATAGTAGAT AAAAGCAGAC TTGGGAGTAA GAACCCTACC 
AAAGGGAAAG TGGAGCAGAC CCAAGCAGAT GAAAAGAAGT TCTACTTCGG CGGCTCACCC 
ATCACTCCCC AGTATGCTAA TTTCACCGGA TGTATAAGTA ATGCCTACTT TACCAGGTTG 
GATAGAGATG TAGAGGTCGA AGATTTCCAG CGGTATTCTG AAAAGGTCCA CACTTCTCTT 
TATGAGTGTC CCATTGAGTC TTCACCATTG TTTCTCCTTC ACAAAAAAGG AAGAAATTCC 
TCAAAGCCTA AAACAAATCA GAATAAAAAG GGAGAGAAAA GTAAAGATGC TCCTTCGTGG 
GATCCTGTTG GCCTGAAATT CCTAGAGAGG AATATTCCAA GAGACTCACA CTGCCAGCTT 
TCCAACAGCC CTAGAGCGAT AGAGCACGCC TATCAGTATG GAGGGACAGC CAACAGTCGT 
CAAGAGTTTG AACACCTAAA AAGAGATTTT GGTGAAAAAT CCCAGTTTTC CATTCGTCTG 
AAAACACGTT CCTCCCATGG GATGATTTTC TATGTCTCAG ATCAAGAAGA GAATAACTTC 
ATGACTCTGT TCTTAGCCCA TGGCCGCTTG GTTTTCATGT TTAATGTTGG CCACAAGAAA 
CTGAAGATTA GAAGCCAGGA GAAATACAAT GATGGACTGT GGCATGATGT GATATTTATT 
CGGGAAAAGA GCAGCGGCCG AATGATAATT GATGGGCTTC GAGTCTTAGA AGAAAGTCTT 
CCCCCTACAG GAGCTGACTG GAAAATCAGG GGTCCTATTT ATTTGGGAGG TGTGGCTCCT 
GGAAGGGCTG TGAAAAATGT CCAGATAAAC TCAGTCTACA GCTTCAGTGG CTGCCTCAGC 
AATCTTCAGC TCAATGGGGC CTCCATCACC TCTGCTTCTC AGACATTTAG TGTGACTCCT 
TGTTTCGAAG GTCCAATGGA AACAGGAACA TACTTCTCAA CAGAAGGAGG TTACGTGGTC 
CTAGATGAGT CTTTCAATAT TGGATTGAAG TTTGAGATTG CATTTGAAGT CCGTCCCAGA 
AGCAGTTCCG GAACCCTTGT TCACGGCCAC AGTGTCAATG GAGAATACCT AAATGTTCAC 
ATGAAAAATG GGCAGGTCAT AGTGAAAGTC AACAATGGCA TCAGAGACTT TTCCACCTCA 
GTGACACCCA AGCAGAGTCT CTGTGATGGC AGATGGCACA GAATTACAGT TATTAGAGAT 
TCAAATGTGG TTCAGTTGGA TGTAGACTCT GAAGTGAACC ACGTGGTTGG ACCCCTAAAT 
CCAAAACCAG TTGATCACAG GGAGCCTGTG TTTGTTGGAG GTGTTCCAGA GTCTCTACTG 
ACACCACGCT TGGCCCCCGG CAGACCCTTC ACAGGCTGCA TCCGTCACTT TGTGATTGAC 
GGGCGCCCGG TGAGCTTCAG TAAAGCAGCC CTGGTCAGCG GCGCCGTGAG CATCAACTCC 
TGTCCAGCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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LAMA4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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