GRIA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRIA1
Entrez Gene ID	529618
Full Name	glutamate ionotropic receptor AMPA type subunit 1
General protein information	Preferred Names glutamate ionotropic receptor AMPA type subunit 1 Names glutamate receptor 1 ionotropic glutamate receptor AMPA 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002689318.5	XP_002689364.1	glutamate receptor 1 isoform X1
NM_001281913.1	NP_001268842.1	glutamate receptor 1 precursor
XM_002689318.6	XP_002689364.2	glutamate receptor 1 isoform X1
XM_024994316.1	XP_024850084.1	glutamate receptor 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRIA1	NP_001107655.1
Canis lupus familiaris (dog)	GRIA1	XP_546286.2
Bos taurus (cattle)	GRIA1	XP_002689365.1
Danio rerio (zebrafish)	gria1b	NP_991293.1
Rattus norvegicus (Norway rat)	Gria1	NP_113796.1
	GRIA1	XP_002804636.1
Mus musculus (house mouse)	Gria1	NP_001106796.1
Gallus gallus (chicken)	GRIA1	NP_001001774.1
Danio rerio (zebrafish)	gria1a	NP_991161.1
Pan troglodytes (chimpanzee)	GRIA1	XP_003310973.1
Xenopus tropicalis (tropical clawed frog)	gria1	XP_002941536.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility.
Cushman RA, Miles JR, Rempel LA, McDaneld TG, Kuehn LA, Chitko-McKown CG, Nonneman D, Echternkamp SE
Journal of animal science91(6)2640-6(2013 Jun)

Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate.
Sugimoto M, Sasaki S, Watanabe T, Nishimura S, Ideta A, Yamazaki M, Matsuda K, Yuzaki M, Sakimura K, Aoyagi Y, Sugimoto Y
PloS one5(11)e13817(2010 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The GRIA1 polymorphism exists in beef cows but it does not influence antral follicle numbers. The association between GnRHR genotype and age at first calving is likely not causal as this polymorphism is not functional.
Title: Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility.

Data show that GRIA1 is a critical mediator of ovulation and that GRIA1 might be a useful target for reproductive therapy.
Title: Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate.

The following GRIA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRIA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa02058	NM_001281913.1 Latest version!	Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa21693	XM_002689318.5 Latest version!	Bos taurus glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa236083	XM_002689318.6 Latest version!	Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa236084	XM_024994316.1 Latest version!	Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRIA1 ( NM_001281913.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001268842.1 Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), mRNA.

Price & Availability↑

CloneID	OBa02058
Clone ID Related Accession (Same CDS sequence)	NM_001281913.1
Accession Version	NM_001281913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	glutamate receptor 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ404792.1. On or before Aug 31, 2013 this sequence version replaced XM_002689319.3, XM_002704887.3. ##Evidence-Data-START## Transcript exon combination :: GQ404792.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GTACACCTCT GCGCTCACCT ACGATGGGGT GAAGGTGATG 
GCTGAGGCTT TCCAGAACCT GCGGAGGCAG AGAATTGATA TCTCCCGCCG GGGGAATGCT 
GGGGACTGCC TGGCTAACCC AGCTGTGCCC TGGGGCCAGG GCATCGACAT CCAGAGAGCT 
CTGCAGCAGG TGCGGTTTGA AGGCTTGACA GGAAATGTGC AATTTAATGA GAAAGGACGG 
CGGACCAACT ACACCCTCCA CGTGATTGAA ATGAAGCATG ATGGCATCCG AAAGATTGGC 
TACTGGAATG AAGATGACAA ATTCGTCCCT GCAGCCACGG ATGCTCAAGC CGGGGGGGAT 
AACTCAAGTG TCCAGAATAG AACATACATC GTCACTACAA TCCTAGAAGA TCCTTATGTG 
ATGCTCAAGA AGAATGCCAA CCAGTTTGAA GGCAATGACC GCTACGAGGG CTACTGTGTG 
GAGTTGGCGG CAGAGATTGC CAAGCATGTG GGATATTCCT ACCGTCTTGA GATTGTCAGC 
GATGGAAAAT ATGGAGCCCG TGACCCTGAC ACAAAGGCTT GGAATGGCAT GGTGGGAGAG 
CTGGTCTATG GAAGAGCAGA TGTGGCTGTT GCCCCTTTAA CTATCACCTT GGTCCGGGAA 
GAGGTGATCG ATTTCTCCAA GCCGTTTATG AGTCTGGGGA TCTCCATCAT GATAAAAAAG 
CCACAGAAGT CCAAACCGGG GGTCTTTTCC TTCCTGGATC CTTTGGCTTA TGAGATCTGG 
ATGTGCATTG TGTTCGCCTA CATTGGAGTG AGCGTCGTCC TTTTCCTGGT CAGCCGTTTC 
AGCCCCTACG AATGGCACAG CGAAGAGTTT GAGGAAGGGC GAGACCAAAC CACCAGCGAC 
CAGTCCAATG AGTTTGGGAT ATTCAACAGC TTGTGGTTCT CCCTGGGAGC CTTCATGCAG 
CAAGGATGTG ACATCTCTCC CAGGTCCTTG TCCGGCCGCA TCGTGGGCGG CGTCTGGTGG 
TTCTTCACCT TGATCATCAT CTCCTCCTAC ACAGCCAACC TGGCCGCCTT CCTGACAGTG 
GAGAGGATGG TGTCTCCCAT CGAGAGTGCT GAGGACCTAG CAAAGCAGAC AGAAATCGCT 
TACGGGACCC TAGAAGCGGG ATCTACGAAG GAGTTCTTCA GGAGGTCTAA AATCGCCGTG 
TTTGAGAAGA TGTGGACGTA CATGAAGTCA GCAGAGCCAT CCGTGTTTGT GCGGACCACA 
GAGGAGGGGA TGATCCGAGT AAGGAAATCC AAAGGCAAAT ACGCCTACCT TCTAGAGTCC 
ACCATGAATG AGTACATTGA GCAGCGGAAA CCCTGTGACA CCATGAAGGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACG CCCAAGGGGT CCGCCCTGAG AGGTCCCGTA 
AACCTAGCGG TTTTGAAACT CAGTGAGCAA GGCGTCTTAG ACAAGCTGAA AAGCAAATGG 
TGGTACGATA AAGGGGAATG TGGAAGCAAG GACTCCGGAA GTAAGGACAA GACCAGTGCT 
CTCAGCCTCA GCAATGTGGC GGGCGTGTTC TACATCCTGA TCGGAGGACT TGGACTCGCC 
ATGCTGGTTG CCTTAATTGA GTTCTGCTAC AAATCCCGTA GCGAATCCAA GCGGATGAAG 
GGTTTCTGTT TGATCCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACGCTCCCC 
CGAAACAGTG GGGCAGGAGC CAGCGGTGCC GGGAGTGGAG AGAACGGCCG GGTAGTCAGC 
CATGACTTCC CCAAGTCCAT GCAATCGATC CCCTGCATGA GCCACAGTTC AGGGATGCCC 
TTGGGAGCCA CTGGATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268842.1
CDS	71..2791
Translation	MQHIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQNLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGAGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	NM_001281913.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281913.1

ORF Insert Sequence:

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GTACACCTCT GCGCTCACCT ACGATGGGGT GAAGGTGATG 
GCTGAGGCTT TCCAGAACCT GCGGAGGCAG AGAATTGATA TCTCCCGCCG GGGGAATGCT 
GGGGACTGCC TGGCTAACCC AGCTGTGCCC TGGGGCCAGG GCATCGACAT CCAGAGAGCT 
CTGCAGCAGG TGCGGTTTGA AGGCTTGACA GGAAATGTGC AATTTAATGA GAAAGGACGG 
CGGACCAACT ACACCCTCCA CGTGATTGAA ATGAAGCATG ATGGCATCCG AAAGATTGGC 
TACTGGAATG AAGATGACAA ATTCGTCCCT GCAGCCACGG ATGCTCAAGC CGGGGGGGAT 
AACTCAAGTG TCCAGAATAG AACATACATC GTCACTACAA TCCTAGAAGA TCCTTATGTG 
ATGCTCAAGA AGAATGCCAA CCAGTTTGAA GGCAATGACC GCTACGAGGG CTACTGTGTG 
GAGTTGGCGG CAGAGATTGC CAAGCATGTG GGATATTCCT ACCGTCTTGA GATTGTCAGC 
GATGGAAAAT ATGGAGCCCG TGACCCTGAC ACAAAGGCTT GGAATGGCAT GGTGGGAGAG 
CTGGTCTATG GAAGAGCAGA TGTGGCTGTT GCCCCTTTAA CTATCACCTT GGTCCGGGAA 
GAGGTGATCG ATTTCTCCAA GCCGTTTATG AGTCTGGGGA TCTCCATCAT GATAAAAAAG 
CCACAGAAGT CCAAACCGGG GGTCTTTTCC TTCCTGGATC CTTTGGCTTA TGAGATCTGG 
ATGTGCATTG TGTTCGCCTA CATTGGAGTG AGCGTCGTCC TTTTCCTGGT CAGCCGTTTC 
AGCCCCTACG AATGGCACAG CGAAGAGTTT GAGGAAGGGC GAGACCAAAC CACCAGCGAC 
CAGTCCAATG AGTTTGGGAT ATTCAACAGC TTGTGGTTCT CCCTGGGAGC CTTCATGCAG 
CAAGGATGTG ACATCTCTCC CAGGTCCTTG TCCGGCCGCA TCGTGGGCGG CGTCTGGTGG 
TTCTTCACCT TGATCATCAT CTCCTCCTAC ACAGCCAACC TGGCCGCCTT CCTGACAGTG 
GAGAGGATGG TGTCTCCCAT CGAGAGTGCT GAGGACCTAG CAAAGCAGAC AGAAATCGCT 
TACGGGACCC TAGAAGCGGG ATCTACGAAG GAGTTCTTCA GGAGGTCTAA AATCGCCGTG 
TTTGAGAAGA TGTGGACGTA CATGAAGTCA GCAGAGCCAT CCGTGTTTGT GCGGACCACA 
GAGGAGGGGA TGATCCGAGT AAGGAAATCC AAAGGCAAAT ACGCCTACCT TCTAGAGTCC 
ACCATGAATG AGTACATTGA GCAGCGGAAA CCCTGTGACA CCATGAAGGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACG CCCAAGGGGT CCGCCCTGAG AGGTCCCGTA 
AACCTAGCGG TTTTGAAACT CAGTGAGCAA GGCGTCTTAG ACAAGCTGAA AAGCAAATGG 
TGGTACGATA AAGGGGAATG TGGAAGCAAG GACTCCGGAA GTAAGGACAA GACCAGTGCT 
CTCAGCCTCA GCAATGTGGC GGGCGTGTTC TACATCCTGA TCGGAGGACT TGGACTCGCC 
ATGCTGGTTG CCTTAATTGA GTTCTGCTAC AAATCCCGTA GCGAATCCAA GCGGATGAAG 
GGTTTCTGTT TGATCCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACGCTCCCC 
CGAAACAGTG GGGCAGGAGC CAGCGGTGCC GGGAGTGGAG AGAACGGCCG GGTAGTCAGC 
CATGACTTCC CCAAGTCCAT GCAATCGATC CCCTGCATGA GCCACAGTTC AGGGATGCCC 
TTGGGAGCCA CTGGATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIA1 ( XM_002689318.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689364.1 Bos taurus glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21693
Clone ID Related Accession (Same CDS sequence)	XM_002689318.5
Accession Version	XM_002689318.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glutamate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689318.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GTACACCTCT GCGCTCACCT ACGATGGGGT GAAGGTGATG 
GCTGAGGCTT TCCAGAACCT GCGGAGGCAG AGAATTGATA TCTCCCGCCG GGGGAATGCT 
GGGGACTGCC TGGCTAACCC AGCTGTGCCC TGGGGCCAGG GCATCGACAT CCAGAGAGCT 
CTGCAGCAGG TGCGGTTTGA AGGCTTGACA GGAAATGTGC AATTTAATGA GAAAGGACGG 
CGGACCAACT ACACCCTCCA CGTGATTGAA ATGAAGCATG ATGGCATCCG AAAGATTGGC 
TACTGGAATG AAGATGACAA ATTCGTCCCT GCAGCCACGG ATGCTCAAGC CGGGGGGGAT 
AACTCAAGTG TCCAGAATAG AACATACATC GTCACTACAA TCCTAGAAGA TCCTTATGTG 
ATGCTCAAGA AGAATGCCAA CCAGTTTGAA GGCAATGACC GCTACGAGGG CTACTGTGTG 
GAGTTGGCGG CAGAGATTGC CAAGCATGTG GGATATTCCT ACCGTCTTGA GATTGTCAGC 
GATGGAAAAT ATGGAGCCCG TGACCCTGAC ACAAAGGCTT GGAATGGCAT GGTGGGAGAG 
CTGGTCTATG GAAGAGCAGA TGTGGCTGTT GCCCCTTTAA CTATCACCTT GGTCCGGGAA 
GAGGTGATCG ATTTCTCCAA GCCGTTTATG AGTCTGGGGA TCTCCATCAT GATAAAAAAG 
CCACAGAAGT CCAAACCGGG GGTCTTTTCC TTCCTGGATC CTTTGGCTTA TGAGATCTGG 
ATGTGCATTG TGTTCGCCTA CATTGGAGTG AGCGTCGTCC TTTTCCTGGT CAGCCGTTTC 
AGCCCCTACG AATGGCACAG CGAAGAGTTT GAGGAAGGGC GAGACCAAAC CACCAGCGAC 
CAGTCCAATG AGTTTGGGAT ATTCAACAGC TTGTGGTTCT CCCTGGGAGC CTTCATGCAG 
CAAGGATGTG ACATCTCTCC CAGGTCCTTG TCCGGCCGCA TCGTGGGCGG CGTCTGGTGG 
TTCTTCACCT TGATCATCAT CTCCTCCTAC ACAGCCAACC TGGCCGCCTT CCTGACAGTG 
GAGAGGATGG TGTCTCCCAT CGAGAGTGCT GAGGACCTAG CAAAGCAGAC AGAAATCGCT 
TACGGGACCC TAGAAGCGGG ATCTACGAAG GAGTTCTTCA GGAGGTCTAA AATCGCCGTG 
TTTGAGAAGA TGTGGACGTA CATGAAGTCA GCAGAGCCAT CCGTGTTTGT GCGGACCACA 
GAGGAGGGGA TGATCCGAGT AAGGAAATCC AAAGGCAAAT ACGCCTACCT TCTAGAGTCC 
ACCATGAATG AGTACATTGA GCAGCGGAAA CCCTGTGACA CCATGAAGGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACG CCCAAGGGGT CCGCCCTGAG AAATCCAGTA 
AACCTGGCAG TGTTAAAACT GAACGAGCAG GGGCTTTTGG ACAAATTGAA AAACAAATGG 
TGGTACGACA AGGGCGAGTG CGGCAGCGGG GGAGGTGATT CCAAGGACAA GACCAGTGCT 
CTCAGCCTCA GCAATGTGGC GGGCGTGTTC TACATCCTGA TCGGAGGACT TGGACTCGCC 
ATGCTGGTTG CCTTAATTGA GTTCTGCTAC AAATCCCGTA GCGAATCCAA GCGGATGAAG 
GGTTTCTGTT TGATCCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACGCTCCCC 
CGAAACAGTG GGGCAGGAGC CAGCGGTGCC GGGAGTGGAG AGAACGGCCG GGTAGTCAGC 
CATGACTTCC CCAAGTCCAT GCAATCGATC CCCTGCATGA GCCACAGTTC AGGGATGCCC 
TTGGGAGCCA CTGGATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689364.1
CDS	315..3035
Translation	MQHIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQNLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGAGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	XM_002689318.5
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689318.5

ORF Insert Sequence:

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GTACACCTCT GCGCTCACCT ACGATGGGGT GAAGGTGATG 
GCTGAGGCTT TCCAGAACCT GCGGAGGCAG AGAATTGATA TCTCCCGCCG GGGGAATGCT 
GGGGACTGCC TGGCTAACCC AGCTGTGCCC TGGGGCCAGG GCATCGACAT CCAGAGAGCT 
CTGCAGCAGG TGCGGTTTGA AGGCTTGACA GGAAATGTGC AATTTAATGA GAAAGGACGG 
CGGACCAACT ACACCCTCCA CGTGATTGAA ATGAAGCATG ATGGCATCCG AAAGATTGGC 
TACTGGAATG AAGATGACAA ATTCGTCCCT GCAGCCACGG ATGCTCAAGC CGGGGGGGAT 
AACTCAAGTG TCCAGAATAG AACATACATC GTCACTACAA TCCTAGAAGA TCCTTATGTG 
ATGCTCAAGA AGAATGCCAA CCAGTTTGAA GGCAATGACC GCTACGAGGG CTACTGTGTG 
GAGTTGGCGG CAGAGATTGC CAAGCATGTG GGATATTCCT ACCGTCTTGA GATTGTCAGC 
GATGGAAAAT ATGGAGCCCG TGACCCTGAC ACAAAGGCTT GGAATGGCAT GGTGGGAGAG 
CTGGTCTATG GAAGAGCAGA TGTGGCTGTT GCCCCTTTAA CTATCACCTT GGTCCGGGAA 
GAGGTGATCG ATTTCTCCAA GCCGTTTATG AGTCTGGGGA TCTCCATCAT GATAAAAAAG 
CCACAGAAGT CCAAACCGGG GGTCTTTTCC TTCCTGGATC CTTTGGCTTA TGAGATCTGG 
ATGTGCATTG TGTTCGCCTA CATTGGAGTG AGCGTCGTCC TTTTCCTGGT CAGCCGTTTC 
AGCCCCTACG AATGGCACAG CGAAGAGTTT GAGGAAGGGC GAGACCAAAC CACCAGCGAC 
CAGTCCAATG AGTTTGGGAT ATTCAACAGC TTGTGGTTCT CCCTGGGAGC CTTCATGCAG 
CAAGGATGTG ACATCTCTCC CAGGTCCTTG TCCGGCCGCA TCGTGGGCGG CGTCTGGTGG 
TTCTTCACCT TGATCATCAT CTCCTCCTAC ACAGCCAACC TGGCCGCCTT CCTGACAGTG 
GAGAGGATGG TGTCTCCCAT CGAGAGTGCT GAGGACCTAG CAAAGCAGAC AGAAATCGCT 
TACGGGACCC TAGAAGCGGG ATCTACGAAG GAGTTCTTCA GGAGGTCTAA AATCGCCGTG 
TTTGAGAAGA TGTGGACGTA CATGAAGTCA GCAGAGCCAT CCGTGTTTGT GCGGACCACA 
GAGGAGGGGA TGATCCGAGT AAGGAAATCC AAAGGCAAAT ACGCCTACCT TCTAGAGTCC 
ACCATGAATG AGTACATTGA GCAGCGGAAA CCCTGTGACA CCATGAAGGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCAACG CCCAAGGGGT CCGCCCTGAG AAATCCAGTA 
AACCTGGCAG TGTTAAAACT GAACGAGCAG GGGCTTTTGG ACAAATTGAA AAACAAATGG 
TGGTACGACA AGGGCGAGTG CGGCAGCGGG GGAGGTGATT CCAAGGACAA GACCAGTGCT 
CTCAGCCTCA GCAATGTGGC GGGCGTGTTC TACATCCTGA TCGGAGGACT TGGACTCGCC 
ATGCTGGTTG CCTTAATTGA GTTCTGCTAC AAATCCCGTA GCGAATCCAA GCGGATGAAG 
GGTTTCTGTT TGATCCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACGCTCCCC 
CGAAACAGTG GGGCAGGAGC CAGCGGTGCC GGGAGTGGAG AGAACGGCCG GGTAGTCAGC 
CATGACTTCC CCAAGTCCAT GCAATCGATC CCCTGCATGA GCCACAGTTC AGGGATGCCC 
TTGGGAGCCA CTGGATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIA1 ( XM_002689318.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689364.2 Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236083
Clone ID Related Accession (Same CDS sequence)	XM_002689318.6
Accession Version	XM_002689318.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glutamate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689318.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GTACACCTCT GCGCTCACCT ACGATGGGGT GAAGGTGATG 
GCTGAGGCTT TCCAGAGCCT GCGGAGGCAG AGAATTGATA TCTCCCGCCG GGGGAATGCT 
GGGGACTGCC TGGCTAACCC AGCTGTGCCC TGGGGCCAGG GCATCGACAT CCAGAGAGCT 
CTGCAGCAGG TGCGGTTTGA AGGCTTGACA GGAAATGTGC AATTTAATGA GAAAGGACGG 
CGGACCAACT ACACCCTCCA CGTGATTGAA ATGAAGCATG ATGGCATCCG AAAGATTGGC 
TACTGGAATG AAGATGACAA ATTCGTCCCT GCAGCCACGG ATGCTCAAGC CGGGGGGGAT 
AACTCAAGTG TCCAGAATAG AACATACATC GTCACTACAA TCCTAGAAGA TCCTTATGTG 
ATGCTCAAGA AGAATGCCAA CCAGTTTGAA GGCAATGACC GCTACGAGGG CTACTGTGTG 
GAGTTGGCGG CAGAGATTGC CAAGCATGTG GGATATTCCT ACCGTCTTGA GATTGTCAGC 
GATGGAAAAT ATGGAGCCCG TGACCCTGAC ACAAAGGCTT GGAATGGCAT GGTGGGAGAG 
CTGGTCTATG GAAGAGCAGA TGTGGCTGTT GCCCCTTTAA CTATCACCTT GGTCCGGGAA 
GAGGTGATCG ATTTCTCCAA GCCGTTTATG AGTCTGGGGA TCTCCATCAT GATAAAAAAG 
CCACAGAAGT CCAAACCGGG GGTCTTTTCC TTCCTGGATC CTTTGGCTTA TGAGATCTGG 
ATGTGCATTG TGTTCGCCTA CATTGGAGTG AGCGTCGTCC TTTTCCTGGT CAGCCGTTTC 
AGCCCCTACG AATGGCACAG CGAAGAGTTT GAGGAAGGGC GAGACCAAAC CACCAGCGAC 
CAGTCCAATG AGTTTGGGAT ATTCAACAGC TTGTGGTTCT CCCTGGGAGC CTTCATGCAG 
CAAGGATGTG ACATCTCTCC CAGGTCCTTG TCCGGCCGCA TCGTGGGCGG CGTCTGGTGG 
TTCTTCACCT TGATCATCAT CTCCTCCTAC ACAGCCAACC TGGCCGCCTT CCTGACAGTG 
GAGAGGATGG TGTCTCCCAT CGAGAGTGCT GAGGACCTAG CAAAGCAGAC AGAAATCGCT 
TACGGGACCC TAGAAGCGGG ATCTACGAAG GAGTTCTTCA GGAGGTCTAA AATCGCCGTG 
TTTGAGAAGA TGTGGACGTA CATGAAGTCA GCAGAGCCAT CCGTGTTTGT GCGGACCACA 
GAGGAGGGGA TGATCCGAGT AAGGAAATCC AAAGGCAAAT ACGCCTACCT TCTAGAGTCC 
ACCATGAATG AGTACATTGA GCAGCGGAAA CCCTGCGACA CCATGAAGGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCGACG CCCAAGGGGT CCGCCCTGAG AAATCCAGTA 
AACCTGGCAG TGTTAAAACT GAACGAGCAG GGGCTTTTGG ACAAATTGAA AAACAAATGG 
TGGTACGACA AGGGCGAGTG CGGCAGCGGG GGAGGTGATT CCAAGGACAA GACCAGTGCT 
CTCAGCCTCA GCAATGTGGC GGGCGTGTTC TACATCCTGA TCGGAGGACT TGGACTCGCC 
ATGCTGGTTG CCTTAATTGA GTTCTGCTAC AAATCCCGTA GCGAATCCAA GCGGATGAAG 
GGTTTCTGTT TGATCCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACGCTCCCC 
CGAAACAGTG GGGCAGGAGC CAGCGGTGCC GGGAGTGGAG AGAACGGCCG GGTAGTCAGC 
CATGACTTCC CCAAGTCCAT GCAATCGATC CCCTGCATGA GCCACAGTTC AGGGATGCCC 
TTGGGAGCCA CTGGATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689364.2
CDS	310..3030
Translation	MQHIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGAGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	XM_002689318.6
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689318.6

ORF Insert Sequence:

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GTACACCTCT GCGCTCACCT ACGATGGGGT GAAGGTGATG 
GCTGAGGCTT TCCAGAGCCT GCGGAGGCAG AGAATTGATA TCTCCCGCCG GGGGAATGCT 
GGGGACTGCC TGGCTAACCC AGCTGTGCCC TGGGGCCAGG GCATCGACAT CCAGAGAGCT 
CTGCAGCAGG TGCGGTTTGA AGGCTTGACA GGAAATGTGC AATTTAATGA GAAAGGACGG 
CGGACCAACT ACACCCTCCA CGTGATTGAA ATGAAGCATG ATGGCATCCG AAAGATTGGC 
TACTGGAATG AAGATGACAA ATTCGTCCCT GCAGCCACGG ATGCTCAAGC CGGGGGGGAT 
AACTCAAGTG TCCAGAATAG AACATACATC GTCACTACAA TCCTAGAAGA TCCTTATGTG 
ATGCTCAAGA AGAATGCCAA CCAGTTTGAA GGCAATGACC GCTACGAGGG CTACTGTGTG 
GAGTTGGCGG CAGAGATTGC CAAGCATGTG GGATATTCCT ACCGTCTTGA GATTGTCAGC 
GATGGAAAAT ATGGAGCCCG TGACCCTGAC ACAAAGGCTT GGAATGGCAT GGTGGGAGAG 
CTGGTCTATG GAAGAGCAGA TGTGGCTGTT GCCCCTTTAA CTATCACCTT GGTCCGGGAA 
GAGGTGATCG ATTTCTCCAA GCCGTTTATG AGTCTGGGGA TCTCCATCAT GATAAAAAAG 
CCACAGAAGT CCAAACCGGG GGTCTTTTCC TTCCTGGATC CTTTGGCTTA TGAGATCTGG 
ATGTGCATTG TGTTCGCCTA CATTGGAGTG AGCGTCGTCC TTTTCCTGGT CAGCCGTTTC 
AGCCCCTACG AATGGCACAG CGAAGAGTTT GAGGAAGGGC GAGACCAAAC CACCAGCGAC 
CAGTCCAATG AGTTTGGGAT ATTCAACAGC TTGTGGTTCT CCCTGGGAGC CTTCATGCAG 
CAAGGATGTG ACATCTCTCC CAGGTCCTTG TCCGGCCGCA TCGTGGGCGG CGTCTGGTGG 
TTCTTCACCT TGATCATCAT CTCCTCCTAC ACAGCCAACC TGGCCGCCTT CCTGACAGTG 
GAGAGGATGG TGTCTCCCAT CGAGAGTGCT GAGGACCTAG CAAAGCAGAC AGAAATCGCT 
TACGGGACCC TAGAAGCGGG ATCTACGAAG GAGTTCTTCA GGAGGTCTAA AATCGCCGTG 
TTTGAGAAGA TGTGGACGTA CATGAAGTCA GCAGAGCCAT CCGTGTTTGT GCGGACCACA 
GAGGAGGGGA TGATCCGAGT AAGGAAATCC AAAGGCAAAT ACGCCTACCT TCTAGAGTCC 
ACCATGAATG AGTACATTGA GCAGCGGAAA CCCTGCGACA CCATGAAGGT GGGAGGTAAC 
TTGGATTCCA AAGGCTATGG CATTGCGACG CCCAAGGGGT CCGCCCTGAG AAATCCAGTA 
AACCTGGCAG TGTTAAAACT GAACGAGCAG GGGCTTTTGG ACAAATTGAA AAACAAATGG 
TGGTACGACA AGGGCGAGTG CGGCAGCGGG GGAGGTGATT CCAAGGACAA GACCAGTGCT 
CTCAGCCTCA GCAATGTGGC GGGCGTGTTC TACATCCTGA TCGGAGGACT TGGACTCGCC 
ATGCTGGTTG CCTTAATTGA GTTCTGCTAC AAATCCCGTA GCGAATCCAA GCGGATGAAG 
GGTTTCTGTT TGATCCCACA GCAATCCATC AATGAAGCCA TACGGACATC GACGCTCCCC 
CGAAACAGTG GGGCAGGAGC CAGCGGTGCC GGGAGTGGAG AGAACGGCCG GGTAGTCAGC 
CATGACTTCC CCAAGTCCAT GCAATCGATC CCCTGCATGA GCCACAGTTC AGGGATGCCC 
TTGGGAGCCA CTGGATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRIA1 ( XM_024994316.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850084.1 Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236084
Clone ID Related Accession (Same CDS sequence)	XM_024994316.1
Accession Version	XM_024994316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glutamate receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GGTGCGGTTT GAAGGCTTGA CAGGAAATGT GCAATTTAAT 
GAGAAAGGAC GGCGGACCAA CTACACCCTC CACGTGATTG AAATGAAGCA TGATGGCATC 
CGAAAGATTG GCTACTGGAA TGAAGATGAC AAATTCGTCC CTGCAGCCAC GGATGCTCAA 
GCCGGGGGGG ATAACTCAAG TGTCCAGAAT AGAACATACA TCGTCACTAC AATCCTAGAA 
GATCCTTATG TGATGCTCAA GAAGAATGCC AACCAGTTTG AAGGCAATGA CCGCTACGAG 
GGCTACTGTG TGGAGTTGGC GGCAGAGATT GCCAAGCATG TGGGATATTC CTACCGTCTT 
GAGATTGTCA GCGATGGAAA ATATGGAGCC CGTGACCCTG ACACAAAGGC TTGGAATGGC 
ATGGTGGGAG AGCTGGTCTA TGGAAGAGCA GATGTGGCTG TTGCCCCTTT AACTATCACC 
TTGGTCCGGG AAGAGGTGAT CGATTTCTCC AAGCCGTTTA TGAGTCTGGG GATCTCCATC 
ATGATAAAAA AGCCACAGAA GTCCAAACCG GGGGTCTTTT CCTTCCTGGA TCCTTTGGCT 
TATGAGATCT GGATGTGCAT TGTGTTCGCC TACATTGGAG TGAGCGTCGT CCTTTTCCTG 
GTCAGCCGTT TCAGCCCCTA CGAATGGCAC AGCGAAGAGT TTGAGGAAGG GCGAGACCAA 
ACCACCAGCG ACCAGTCCAA TGAGTTTGGG ATATTCAACA GCTTGTGGTT CTCCCTGGGA 
GCCTTCATGC AGCAAGGATG TGACATCTCT CCCAGGTCCT TGTCCGGCCG CATCGTGGGC 
GGCGTCTGGT GGTTCTTCAC CTTGATCATC ATCTCCTCCT ACACAGCCAA CCTGGCCGCC 
TTCCTGACAG TGGAGAGGAT GGTGTCTCCC ATCGAGAGTG CTGAGGACCT AGCAAAGCAG 
ACAGAAATCG CTTACGGGAC CCTAGAAGCG GGATCTACGA AGGAGTTCTT CAGGAGGTCT 
AAAATCGCCG TGTTTGAGAA GATGTGGACG TACATGAAGT CAGCAGAGCC ATCCGTGTTT 
GTGCGGACCA CAGAGGAGGG GATGATCCGA GTAAGGAAAT CCAAAGGCAA ATACGCCTAC 
CTTCTAGAGT CCACCATGAA TGAGTACATT GAGCAGCGGA AACCCTGCGA CACCATGAAG 
GTGGGAGGTA ACTTGGATTC CAAAGGCTAT GGCATTGCGA CGCCCAAGGG GTCCGCCCTG 
AGAGGTCCCG TAAACCTAGC GGTTTTGAAA CTCAGTGAGC AAGGCGTCTT AGACAAGCTG 
AAAAGCAAAT GGTGGTACGA TAAAGGGGAA TGTGGAAGCA AGGACTCCGG AAGTAAGGAC 
AAGACCAGTG CTCTCAGCCT CAGCAATGTG GCGGGCGTGT TCTACATCCT GATCGGAGGA 
CTTGGACTCG CCATGCTGGT TGCCTTAATT GAGTTCTGCT ACAAATCCCG TAGCGAATCC 
AAGCGGATGA AGGGTTTCTG TTTGATCCCA CAGCAATCCA TCAATGAAGC CATACGGACA 
TCGACGCTCC CCCGAAACAG TGGGGCAGGA GCCAGCGGTG CCGGGAGTGG AGAGAACGGC 
CGGGTAGTCA GCCATGACTT CCCCAAGTCC ATGCAATCGA TCCCCTGCAT GAGCCACAGT 
TCAGGGATGC CCTTGGGAGC CACTGGATTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850084.1
CDS	310..2862
Translation	MQHIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGAGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL

Target ORF information:

RefSeq Version	XM_024994316.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate ionotropic receptor AMPA type subunit 1 (GRIA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994316.1

ORF Insert Sequence:

ATGCAGCACA TTTTTGCCTT CTTCTGCACC GGTTTCCTAG GCGCGGTAGT AGGTGCCAAT 
TTCCCCAACA ATATCCAGAT CGGGGGATTA TTTCCAAACC AGCAGTCACA GGAACATGCT 
GCTTTTAGAT TTGCTTTGTC ACAACTCACA GAGCCCCCAA AGTTGCTCCC CCAGATTGAT 
ATTGTGAACA TCAGCGACAG CTTTGAGATG ACCTACAGAT TCTGTTCCCA GTTCTCTAAA 
GGAGTCTATG CCATCTTTGG GTTTTATGAA CGGAGGACTG TCAACATGCT GACCTCCTTC 
TGCGGGGCCC TCCACGTCTG CTTCATTACA CCAAGCTTTC CCGTTGACAC ATCCAACCAG 
TTTGTCCTCC AGCTGCGCCC CGAGCTGCAG GATGCCCTCA TCAGTATCAT CGACCATTAC 
AAGTGGCAAA AATTTGTCTA CATTTATGAT GCTGACCGGG GTCTGTCCGT CTTGCAGAAA 
GTCCTGGACA CAGCTGCTGA GAAGAACTGG CAGGTGACAG CCGTGAACAT TTTGACAACC 
ACGGAGGAGG GTTACCGGAT GCTCTTCCAG GACCTGGAGA AGAAAAAGGA GCGGCTGGTG 
GTGGTGGACT GTGAATCAGA ACGCCTCAAT GCTATCCTGG GCCAGATTGT AAAGCTGGAG 
AAGAATGGCA TTGGCTACCA CTATATCCTT GCAAATCTGG GTTTCATGGA CATTGACTTA 
AACAAATTCA AGGAGAGTGG AGCAAACGTA ACAGGTTTCC AGTTGGTGAA CTACACAGAC 
ACCATCCCGG CCAAGATCAT GCAACAGTGG AAGAACAGTG ATGCTCGGGA CCACACCCGG 
GTGGACTGGA AGAGACCTAA GGTGCGGTTT GAAGGCTTGA CAGGAAATGT GCAATTTAAT 
GAGAAAGGAC GGCGGACCAA CTACACCCTC CACGTGATTG AAATGAAGCA TGATGGCATC 
CGAAAGATTG GCTACTGGAA TGAAGATGAC AAATTCGTCC CTGCAGCCAC GGATGCTCAA 
GCCGGGGGGG ATAACTCAAG TGTCCAGAAT AGAACATACA TCGTCACTAC AATCCTAGAA 
GATCCTTATG TGATGCTCAA GAAGAATGCC AACCAGTTTG AAGGCAATGA CCGCTACGAG 
GGCTACTGTG TGGAGTTGGC GGCAGAGATT GCCAAGCATG TGGGATATTC CTACCGTCTT 
GAGATTGTCA GCGATGGAAA ATATGGAGCC CGTGACCCTG ACACAAAGGC TTGGAATGGC 
ATGGTGGGAG AGCTGGTCTA TGGAAGAGCA GATGTGGCTG TTGCCCCTTT AACTATCACC 
TTGGTCCGGG AAGAGGTGAT CGATTTCTCC AAGCCGTTTA TGAGTCTGGG GATCTCCATC 
ATGATAAAAA AGCCACAGAA GTCCAAACCG GGGGTCTTTT CCTTCCTGGA TCCTTTGGCT 
TATGAGATCT GGATGTGCAT TGTGTTCGCC TACATTGGAG TGAGCGTCGT CCTTTTCCTG 
GTCAGCCGTT TCAGCCCCTA CGAATGGCAC AGCGAAGAGT TTGAGGAAGG GCGAGACCAA 
ACCACCAGCG ACCAGTCCAA TGAGTTTGGG ATATTCAACA GCTTGTGGTT CTCCCTGGGA 
GCCTTCATGC AGCAAGGATG TGACATCTCT CCCAGGTCCT TGTCCGGCCG CATCGTGGGC 
GGCGTCTGGT GGTTCTTCAC CTTGATCATC ATCTCCTCCT ACACAGCCAA CCTGGCCGCC 
TTCCTGACAG TGGAGAGGAT GGTGTCTCCC ATCGAGAGTG CTGAGGACCT AGCAAAGCAG 
ACAGAAATCG CTTACGGGAC CCTAGAAGCG GGATCTACGA AGGAGTTCTT CAGGAGGTCT 
AAAATCGCCG TGTTTGAGAA GATGTGGACG TACATGAAGT CAGCAGAGCC ATCCGTGTTT 
GTGCGGACCA CAGAGGAGGG GATGATCCGA GTAAGGAAAT CCAAAGGCAA ATACGCCTAC 
CTTCTAGAGT CCACCATGAA TGAGTACATT GAGCAGCGGA AACCCTGCGA CACCATGAAG 
GTGGGAGGTA ACTTGGATTC CAAAGGCTAT GGCATTGCGA CGCCCAAGGG GTCCGCCCTG 
AGAGGTCCCG TAAACCTAGC GGTTTTGAAA CTCAGTGAGC AAGGCGTCTT AGACAAGCTG 
AAAAGCAAAT GGTGGTACGA TAAAGGGGAA TGTGGAAGCA AGGACTCCGG AAGTAAGGAC 
AAGACCAGTG CTCTCAGCCT CAGCAATGTG GCGGGCGTGT TCTACATCCT GATCGGAGGA 
CTTGGACTCG CCATGCTGGT TGCCTTAATT GAGTTCTGCT ACAAATCCCG TAGCGAATCC 
AAGCGGATGA AGGGTTTCTG TTTGATCCCA CAGCAATCCA TCAATGAAGC CATACGGACA 
TCGACGCTCC CCCGAAACAG TGGGGCAGGA GCCAGCGGTG CCGGGAGTGG AGAGAACGGC 
CGGGTAGTCA GCCATGACTT CCCCAAGTCC ATGCAATCGA TCCCCTGCAT GAGCCACAGT 
TCAGGGATGC CCTTGGGAGC CACTGGATTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility.
Journal of animal science91(6)2640-6(2013 Jun)
Cushman RA,Miles JR,Rempel LA,McDaneld TG,Kuehn LA,Chitko-McKown CG,Nonneman D,Echternkamp SE

Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate.
PloS one5(11)e13817(2010 Nov)
Sugimoto M,Sasaki S,Watanabe T,Nishimura S,Ideta A,Yamazaki M,Matsuda K,Yuzaki M,Sakimura K,Aoyagi Y,Sugimoto Y

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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GRIA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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