KCNT1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015473688.1	XP_015329174.1	potassium channel subfamily T member 1 isoform X2
XM_010810539.2	XP_010808841.2	potassium channel subfamily T member 1 isoform X5
XM_010819338.2	XP_010817640.2	potassium channel subfamily T member 1 isoform X3
XM_015465525.1	XP_015321011.1	potassium channel subfamily T member 1 isoform X4
XM_015465528.1	XP_015321014.1	potassium channel subfamily T member 1 isoform X7
XM_015465529.1	XP_015321015.1	potassium channel subfamily T member 1 isoform X8
XM_024999623.1	XP_024855391.1	potassium channel subfamily T member 1 isoform X10
XM_024999624.1	XP_024855392.1	potassium channel subfamily T member 1 isoform X11
XM_024999629.1	XP_024855397.1	potassium channel subfamily T member 1 isoform X17
XM_015473691.1	XP_015329177.1	potassium channel subfamily T member 1 isoform X7
XM_015473693.1	XP_015329179.1	potassium channel subfamily T member 1 isoform X8
XM_015465523.1	XP_015321009.1	potassium channel subfamily T member 1 isoform X1
XM_015465524.1	XP_015321010.1	potassium channel subfamily T member 1 isoform X2
XM_015465526.1	XP_015321012.1	potassium channel subfamily T member 1 isoform X6
XM_015465527.1	XP_015321013.1	potassium channel subfamily T member 1 isoform X7
XM_015473688.2	XP_015329174.2	potassium channel subfamily T member 1 isoform X2
XM_024999630.1	XP_024855398.1	potassium channel subfamily T member 1 isoform X18
XM_015473687.1	XP_015329173.1	potassium channel subfamily T member 1 isoform X1
XM_010810537.2	XP_010808839.2	potassium channel subfamily T member 1 isoform X3
XM_015473689.1	XP_015329175.1	potassium channel subfamily T member 1 isoform X4
XM_010819346.2	XP_010817648.2	potassium channel subfamily T member 1 isoform X5
XM_024999617.1	XP_024855385.1	potassium channel subfamily T member 1 isoform X1
XM_015473689.2	XP_015329175.2	potassium channel subfamily T member 1 isoform X4
XM_024999622.1	XP_024855390.1	potassium channel subfamily T member 1 isoform X9
XM_024999625.1	XP_024855393.1	potassium channel subfamily T member 1 isoform X12
XM_024999626.1	XP_024855394.1	potassium channel subfamily T member 1 isoform X13
XM_015473690.1	XP_015329176.1	potassium channel subfamily T member 1 isoform X6
XM_015473692.1	XP_015329178.1	potassium channel subfamily T member 1 isoform X7
XM_024999618.1	XP_024855386.1	potassium channel subfamily T member 1 isoform X3
XM_024999620.1	XP_024855388.1	potassium channel subfamily T member 1 isoform X7
XM_015473691.2	XP_015329177.2	potassium channel subfamily T member 1 isoform X14
XM_024999627.1	XP_024855395.1	potassium channel subfamily T member 1 isoform X15
XM_024999628.1	XP_024855396.1	potassium channel subfamily T member 1 isoform X16
XM_024999631.1	XP_024855399.1	potassium channel subfamily T member 1 isoform X19
XM_015473690.2	XP_015329176.2	potassium channel subfamily T member 1 isoform X5
XM_024999619.1	XP_024855387.1	potassium channel subfamily T member 1 isoform X6
XM_024999621.1	XP_024855389.1	potassium channel subfamily T member 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220961	XM_024999617.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220962	XM_015473688.2 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220963	XM_024999618.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220964	XM_015473689.2 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220965	XM_015473690.2 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220966	XM_024999619.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220967	XM_024999620.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220968	XM_024999621.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220969	XM_024999622.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220970	XM_024999623.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220971	XM_024999624.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220972	XM_024999625.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220973	XM_024999626.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220974	XM_015473691.2 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220975	XM_024999627.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220976	XM_024999628.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa220977	XM_024999629.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220978	XM_024999630.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa220979	XM_024999631.1 Latest version!	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa92023	XM_015473687.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa92023	XM_015465523.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa92024	XM_015473688.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa92024	XM_015465524.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa92025	XM_010819338.2 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92025	XM_010810537.2 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92026	XM_015473689.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92026	XM_015465525.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92027	XM_010810539.2 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92027	XM_010819346.2 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92028	XM_015473690.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92028	XM_015465526.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92029	XM_015473692.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92029	XM_015473691.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92029	XM_015465527.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92029	XM_015465528.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92030	XM_015473693.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa92030	XM_015465529.1 Latest version!	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

KCNT1 ( XM_024999617.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855385.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220961
Clone ID Related Accession (Same CDS sequence)	XM_024999617.1
Accession Version	XM_024999617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC 
CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC 
AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG 
AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG 
ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855385.1
CDS	658..4689
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999617.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999617.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC 
CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC 
AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG 
AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG 
ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473688.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329174.2 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220962
Clone ID Related Accession (Same CDS sequence)	XM_015473688.2
Accession Version	XM_015473688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA 
CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC 
TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG 
CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG 
GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC 
TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC 
AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG 
ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC 
GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC 
GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC 
GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG 
GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC 
GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC 
CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC 
TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC 
TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG 
GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT 
GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG 
ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC 
CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGAGG ATGTAGCAAA TTTAACAGCC 
AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG 
AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC 
ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG 
AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329174.2
CDS	658..4683
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473688.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473688.2

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA 
CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC 
TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG 
CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG 
GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC 
TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC 
AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG 
ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC 
GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC 
GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC 
GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG 
GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC 
GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC 
CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC 
TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC 
TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG 
GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT 
GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG 
ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC 
CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGAGG ATGTAGCAAA TTTAACAGCC 
AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG 
AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC 
ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG 
AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999618.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855386.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220963
Clone ID Related Accession (Same CDS sequence)	XM_024999618.1
Accession Version	XM_024999618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGTCCCAG 
GTCTCGGTGA GCGTGGAGGA CTGTGAGGAC ACTCGGGAGG CAAAGGGGCC CTGGGGTGCA 
CGGGTGGGCA CGAGCGGCGG CAGCACCCAC GGCCGGCACA CGGTGGGCAG CGACCCAGCC 
GAGCACCCGC TCCTGCGGCG CAAGAGCCTC CCGTGGGCGC GGAGGCTGAG CCGCAAGGGC 
TCCCGGCACA CGGGGAATGC GGCAGCCGAG TGGATCAGCC AACAGCGGCT CAGCCTGTAC 
CGGCGCTCCG AGAGGCAGGA GCTCTCAGAG CTGGTCAAGA ACCGCATGAA GCACCTGGGG 
CTGCCCACCA CTGGCTACGA GGATGTAGCA AATTTAACAG CCAGTGATGT CATGAATCGG 
GTAAACCTGG GATATTTGCA AGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855386.1
CDS	656..4663
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999618.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999618.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGTCCCAG 
GTCTCGGTGA GCGTGGAGGA CTGTGAGGAC ACTCGGGAGG CAAAGGGGCC CTGGGGTGCA 
CGGGTGGGCA CGAGCGGCGG CAGCACCCAC GGCCGGCACA CGGTGGGCAG CGACCCAGCC 
GAGCACCCGC TCCTGCGGCG CAAGAGCCTC CCGTGGGCGC GGAGGCTGAG CCGCAAGGGC 
TCCCGGCACA CGGGGAATGC GGCAGCCGAG TGGATCAGCC AACAGCGGCT CAGCCTGTAC 
CGGCGCTCCG AGAGGCAGGA GCTCTCAGAG CTGGTCAAGA ACCGCATGAA GCACCTGGGG 
CTGCCCACCA CTGGCTACGA GGATGTAGCA AATTTAACAG CCAGTGATGT CATGAATCGG 
GTAAACCTGG GATATTTGCA AGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473689.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329175.2 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA.

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CloneID	OBa220964
Clone ID Related Accession (Same CDS sequence)	XM_015473689.2
Accession Version	XM_015473689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329175.2
CDS	657..4625
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473689.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473689.2

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473690.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329176.2 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa220965
Clone ID Related Accession (Same CDS sequence)	XM_015473690.2
Accession Version	XM_015473690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA 
GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT 
ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329176.2
CDS	660..4598
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQGSHWPAPGSPPSSQEAPAWSTPGQVTRAPGMVEHSET

Target ORF information:

RefSeq Version	XM_015473690.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473690.2

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA 
GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT 
ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999619.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855387.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa220966
Clone ID Related Accession (Same CDS sequence)	XM_024999619.1
Accession Version	XM_024999619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG 
GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG 
GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC 
TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG 
AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA 
AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGGCC 
TCACCCCTCC TCCCCGGCCA GTCCCAGGTC TCGGTGAGCG TGGAGGACTG TGAGGACACT 
CGGGAGGCAA AGGGGCCCTG GGGTGCACGG GTGGGCACGA GCGGCGGCAG CACCCACGGC 
CGGCACACGG TGGGCAGCGA CCCAGCCGAG CACCCGCTCC TGCGGCGCAA GAGCCTCCCG 
TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC CGGCACACGG GGAATGCGGC AGCCGAGTGG 
ATCAGCCAAC AGCGGCTCAG CCTGTACCGG CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GCATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA TGTAGCAAAT 
TTAACAGCCA GTGATGTCAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA CGAGATGAAT 
GACCACCAGA ACACCCTCTC CTATGTGCTC ATCAACCCCC CGCCCGACAC GCGGCTGGAG 
CCCAATGACA TTGTATATCT CATCCGCTCC GACCCCTTGG CCCACGTGGC CAGCAGCTCC 
CAGAGCCGGA AGAGCAGCTG CAGCAACAAG CTGGCCTCCT GCAACCCTGA GACTCGGGAC 
GAGACACAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855387.1
CDS	9..3743
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999619.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999619.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG 
GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG 
GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC 
TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG 
AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA 
AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGGCC 
TCACCCCTCC TCCCCGGCCA GTCCCAGGTC TCGGTGAGCG TGGAGGACTG TGAGGACACT 
CGGGAGGCAA AGGGGCCCTG GGGTGCACGG GTGGGCACGA GCGGCGGCAG CACCCACGGC 
CGGCACACGG TGGGCAGCGA CCCAGCCGAG CACCCGCTCC TGCGGCGCAA GAGCCTCCCG 
TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC CGGCACACGG GGAATGCGGC AGCCGAGTGG 
ATCAGCCAAC AGCGGCTCAG CCTGTACCGG CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GCATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA TGTAGCAAAT 
TTAACAGCCA GTGATGTCAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA CGAGATGAAT 
GACCACCAGA ACACCCTCTC CTATGTGCTC ATCAACCCCC CGCCCGACAC GCGGCTGGAG 
CCCAATGACA TTGTATATCT CATCCGCTCC GACCCCTTGG CCCACGTGGC CAGCAGCTCC 
CAGAGCCGGA AGAGCAGCTG CAGCAACAAG CTGGCCTCCT GCAACCCTGA GACTCGGGAC 
GAGACACAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999620.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855388.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA.

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CloneID	OBa220967
Clone ID Related Accession (Same CDS sequence)	XM_024999620.1
Accession Version	XM_024999620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG 
GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG 
GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC 
TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG 
AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA 
AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGAGGATGTA GCAAATTTAA CAGCCAGTGA TGTCATGAAT 
CGGGTAAACC TGGGATATTT GCAAGACGAG ATGAATGACC ACCAGAACAC CCTCTCCTAT 
GTGCTCATCA ACCCCCCGCC CGACACGCGG CTGGAGCCCA ATGACATTGT ATATCTCATC 
CGCTCCGACC CCTTGGCCCA CGTGGCCAGC AGCTCCCAGA GCCGGAAGAG CAGCTGCAGC 
AACAAGCTGG CCTCCTGCAA CCCTGAGACT CGGGACGAGA CACAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855388.1
CDS	9..3719
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999620.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999620.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG 
GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG 
GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC 
TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG 
AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA 
AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGAGGATGTA GCAAATTTAA CAGCCAGTGA TGTCATGAAT 
CGGGTAAACC TGGGATATTT GCAAGACGAG ATGAATGACC ACCAGAACAC CCTCTCCTAT 
GTGCTCATCA ACCCCCCGCC CGACACGCGG CTGGAGCCCA ATGACATTGT ATATCTCATC 
CGCTCCGACC CCTTGGCCCA CGTGGCCAGC AGCTCCCAGA GCCGGAAGAG CAGCTGCAGC 
AACAAGCTGG CCTCCTGCAA CCCTGAGACT CGGGACGAGA CACAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999621.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855389.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa220968
Clone ID Related Accession (Same CDS sequence)	XM_024999621.1
Accession Version	XM_024999621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GTGGCCATGG ACCTCCAGAA TACGGAGTGC 
CGACCCACAC AGAGTGGCGG GGGCAGTGGG GGCAGCAAGC TGGCGCTCCC CACGGAGAAC 
GGCTCAGGCA GCCGGCGGCC CAGCATCGCG CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC 
CTGCTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGACG AGATGACGCC GTCGGAGGAT 
GAGGGGCTGT CCGTGGTGGA GTATGTGAAG GGCTACCCTC CTAACTCACC CTACATCGGC 
AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG 
GACAAGGGCT GCAAGCACAA CAGCTACGAA GATGCCAAGG CCTATGGCTT CAAGAACAAG 
CTGATCATCG TCTCAGCGGA GACGGCGGGC AACGGGCTGT ACAACTTCAT CGTGCCGCTG 
CGCGCCTACT ACCGGCCTCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT GGACAACAAG 
CCCGACCACC ACTTCCTGGA GGCCATCTGC TGCTTTCCCA TGGTCTACTA CATGGAGGGC 
TCCGTGGACA ACCTGGACAG CCTGCTGCGG TGCGGTGTCA TCTACGCGGA CAATCTGGTG 
GTGGCAGACA AGGAGAGCAC CATGAGCGCT GAGGAGGACT ACATGGCCGA CGCAAAGACC 
ATCGTCAATG TGCAGACCAT GTTCCGGCTC TTCCCCAGTC TCAGCATCAC CACCGAGCTC 
ACCCACCCGT CCAACATGCG GTTCATGCAG TTTCGCGCCA AGGACAGCTA CTCTCTGGCT 
CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC ATCAGCATGC TGGACACACT GCTGTATCAG 
TCCTTCGTGA AGGACTACAT GATCCCCATC ACCAGGCTGT TGCTGGGCTT GGACACCACG 
CCGGGCTCCG GCTACCTCTG TGCGATGAAG ATCTCGGAGG ACGACCTGTG GATCCGCACC 
TATGGCCGCC TCTTCCAGAA GCTGTGCTCC TCCAGCGCCG AGATCCCTAT CGGCATCTAC 
AGGACCCAGT GCCACGTCTT CTCCACCGAG CCCCCCGACC TCAGGGCCCA GTCCCAGGTC 
TCGGTGAGCG TGGAGGACTG TGAGGACACT CGGGAGGCAA AGGGGCCCTG GGGTGCACGG 
GTGGGCACGA GCGGCGGCAG CACCCACGGC CGGCACACGG TGGGCAGCGA CCCAGCCGAG 
CACCCGCTCC TGCGGCGCAA GAGCCTCCCG TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC 
CGGCACACGG GGAATGCGGC AGCCGAGTGG ATCAGCCAAC AGCGGCTCAG CCTGTACCGG 
CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG GTCAAGAACC GCATGAAGCA CCTGGGGCTG 
CCCACCACTG GCTACGAGGA TGTAGCAAAT TTAACAGCCA GTGATGTCAT GAATCGGGTA 
AACCTGGGAT ATTTGCAAGA CGAGATGAAT GACCACCAGA ACACCCTCTC CTATGTGCTC 
ATCAACCCCC CGCCCGACAC GCGGCTGGAG CCCAATGACA TTGTATATCT CATCCGCTCC 
GACCCCTTGG CCCACGTGGC CAGCAGCTCC CAGAGCCGGA AGAGCAGCTG CAGCAACAAG 
CTGGCCTCCT GCAACCCTGA GACTCGGGAC GAGACACAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855389.1
CDS	9..3713
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999621.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999621.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GTGGCCATGG ACCTCCAGAA TACGGAGTGC 
CGACCCACAC AGAGTGGCGG GGGCAGTGGG GGCAGCAAGC TGGCGCTCCC CACGGAGAAC 
GGCTCAGGCA GCCGGCGGCC CAGCATCGCG CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC 
CTGCTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGACG AGATGACGCC GTCGGAGGAT 
GAGGGGCTGT CCGTGGTGGA GTATGTGAAG GGCTACCCTC CTAACTCACC CTACATCGGC 
AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG 
GACAAGGGCT GCAAGCACAA CAGCTACGAA GATGCCAAGG CCTATGGCTT CAAGAACAAG 
CTGATCATCG TCTCAGCGGA GACGGCGGGC AACGGGCTGT ACAACTTCAT CGTGCCGCTG 
CGCGCCTACT ACCGGCCTCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT GGACAACAAG 
CCCGACCACC ACTTCCTGGA GGCCATCTGC TGCTTTCCCA TGGTCTACTA CATGGAGGGC 
TCCGTGGACA ACCTGGACAG CCTGCTGCGG TGCGGTGTCA TCTACGCGGA CAATCTGGTG 
GTGGCAGACA AGGAGAGCAC CATGAGCGCT GAGGAGGACT ACATGGCCGA CGCAAAGACC 
ATCGTCAATG TGCAGACCAT GTTCCGGCTC TTCCCCAGTC TCAGCATCAC CACCGAGCTC 
ACCCACCCGT CCAACATGCG GTTCATGCAG TTTCGCGCCA AGGACAGCTA CTCTCTGGCT 
CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC 
CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC ATCAGCATGC TGGACACACT GCTGTATCAG 
TCCTTCGTGA AGGACTACAT GATCCCCATC ACCAGGCTGT TGCTGGGCTT GGACACCACG 
CCGGGCTCCG GCTACCTCTG TGCGATGAAG ATCTCGGAGG ACGACCTGTG GATCCGCACC 
TATGGCCGCC TCTTCCAGAA GCTGTGCTCC TCCAGCGCCG AGATCCCTAT CGGCATCTAC 
AGGACCCAGT GCCACGTCTT CTCCACCGAG CCCCCCGACC TCAGGGCCCA GTCCCAGGTC 
TCGGTGAGCG TGGAGGACTG TGAGGACACT CGGGAGGCAA AGGGGCCCTG GGGTGCACGG 
GTGGGCACGA GCGGCGGCAG CACCCACGGC CGGCACACGG TGGGCAGCGA CCCAGCCGAG 
CACCCGCTCC TGCGGCGCAA GAGCCTCCCG TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC 
CGGCACACGG GGAATGCGGC AGCCGAGTGG ATCAGCCAAC AGCGGCTCAG CCTGTACCGG 
CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG GTCAAGAACC GCATGAAGCA CCTGGGGCTG 
CCCACCACTG GCTACGAGGA TGTAGCAAAT TTAACAGCCA GTGATGTCAT GAATCGGGTA 
AACCTGGGAT ATTTGCAAGA CGAGATGAAT GACCACCAGA ACACCCTCTC CTATGTGCTC 
ATCAACCCCC CGCCCGACAC GCGGCTGGAG CCCAATGACA TTGTATATCT CATCCGCTCC 
GACCCCTTGG CCCACGTGGC CAGCAGCTCC CAGAGCCGGA AGAGCAGCTG CAGCAACAAG 
CTGGCCTCCT GCAACCCTGA GACTCGGGAC GAGACACAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999622.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855390.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa220969
Clone ID Related Accession (Same CDS sequence)	XM_024999622.1
Accession Version	XM_024999622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC 
AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG 
CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT 
GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG 
GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG 
GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC 
GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG 
CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG 
AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG 
CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC 
TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT 
TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855390.1
CDS	9..3701
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999622.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999622.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC 
AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG 
CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT 
GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG 
GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG 
GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC 
GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG 
CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG 
AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG 
CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC 
TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT 
TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999623.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855391.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA.

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CloneID	OBa220970
Clone ID Related Accession (Same CDS sequence)	XM_024999623.1
Accession Version	XM_024999623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC 
AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG 
CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT 
GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG 
GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGTCCCA GGTCTCGGTG AGCGTGGAGG ACTGTGAGGA CACTCGGGAG 
GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC ACGAGCGGCG GCAGCACCCA CGGCCGGCAC 
ACGGTGGGCA GCGACCCAGC CGAGCACCCG CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG 
CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC ACGGGGAATG CGGCAGCCGA GTGGATCAGC 
CAACAGCGGC TCAGCCTGTA CCGGCGCTCC GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGCATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGATGTAGC AAATTTAACA 
GCCAGTGATG TCATGAATCG GGTAAACCTG GGATATTTGC AAGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855391.1
CDS	9..3677
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999623.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999623.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC 
AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG 
CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT 
GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG 
GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGTCCCA GGTCTCGGTG AGCGTGGAGG ACTGTGAGGA CACTCGGGAG 
GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC ACGAGCGGCG GCAGCACCCA CGGCCGGCAC 
ACGGTGGGCA GCGACCCAGC CGAGCACCCG CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG 
CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC ACGGGGAATG CGGCAGCCGA GTGGATCAGC 
CAACAGCGGC TCAGCCTGTA CCGGCGCTCC GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGCATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGATGTAGC AAATTTAACA 
GCCAGTGATG TCATGAATCG GGTAAACCTG GGATATTTGC AAGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999624.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855392.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa220971
Clone ID Related Accession (Same CDS sequence)	XM_024999624.1
Accession Version	XM_024999624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG 
GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG 
GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC 
TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG 
AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA 
AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855392.1
CDS	9..3656
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999624.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999624.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG 
TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG 
GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG 
GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC 
TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG 
AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA 
AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999625.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855393.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa220972
Clone ID Related Accession (Same CDS sequence)	XM_024999625.1
Accession Version	XM_024999625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC 
AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG 
CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT 
GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG 
GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG 
GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC 
GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG 
CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG 
AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG 
CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC 
TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT 
TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855393.1
CDS	31..3663
Translation	MSDLDSEVLPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999625.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999625.1

ORF Insert Sequence:

ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC 
AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG 
CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT 
GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG 
GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG 
GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC 
GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG 
CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG 
AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG 
CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC 
TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT 
TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999626.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855394.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA.

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CloneID	OBa220973
Clone ID Related Accession (Same CDS sequence)	XM_024999626.1
Accession Version	XM_024999626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGT GGCCATGGAC 
CTCCAGAATA CGGAGTGCCG ACCCACACAG AGTGGCGGGG GCAGTGGGGG CAGCAAGCTG 
GCGCTCCCCA CGGAGAACGG CTCAGGCAGC CGGCGGCCCA GCATCGCGCC GGTGCTGGAG 
GTGGCTGACA GCTCAGCCCT GCTGCCCTGC GACCTGCTGA GTGACCAGTC AGAGGACGAG 
ATGACGCCGT CGGAGGATGA GGGGCTGTCC GTGGTGGAGT ATGTGAAGGG CTACCCTCCT 
AACTCACCCT ACATCGGCAG CTCCCCAACC CTGTGCCATC TCCTGCCCGT GAAAGCCCCC 
TTCTGCTGCC TGCGGCTGGA CAAGGGCTGC AAGCACAACA GCTACGAAGA TGCCAAGGCC 
TATGGCTTCA AGAACAAGCT GATCATCGTC TCAGCGGAGA CGGCGGGCAA CGGGCTGTAC 
AACTTCATCG TGCCGCTGCG CGCCTACTAC CGGCCTCGCC GGGAGCTCAA CCCCATTGTG 
CTGCTGCTGG ACAACAAGCC CGACCACCAC TTCCTGGAGG CCATCTGCTG CTTTCCCATG 
GTCTACTACA TGGAGGGCTC CGTGGACAAC CTGGACAGCC TGCTGCGGTG CGGTGTCATC 
TACGCGGACA ATCTGGTGGT GGCAGACAAG GAGAGCACCA TGAGCGCTGA GGAGGACTAC 
ATGGCCGACG CAAAGACCAT CGTCAATGTG CAGACCATGT TCCGGCTCTT CCCCAGTCTC 
AGCATCACCA CCGAGCTCAC CCACCCGTCC AACATGCGGT TCATGCAGTT TCGCGCCAAG 
GACAGCTACT CTCTGGCTCT CTCTAAACTG GAGAAGAGGG AGCGTGAGAA CGGCTCCAAC 
CTGGCCTTCA TGTTCCGCCT GCCCTTCGCC GCCGGCCGCG TCTTCAGCAT CAGCATGCTG 
GACACACTGC TGTATCAGTC CTTCGTGAAG GACTACATGA TCCCCATCAC CAGGCTGTTG 
CTGGGCTTGG ACACCACGCC GGGCTCCGGC TACCTCTGTG CGATGAAGAT CTCGGAGGAC 
GACCTGTGGA TCCGCACCTA TGGCCGCCTC TTCCAGAAGC TGTGCTCCTC CAGCGCCGAG 
ATCCCTATCG GCATCTACAG GACCCAGTGC CACGTCTTCT CCACCGAGCC CCCCGACCTC 
AGGGCCCAGT CCCAGGTCTC GGTGAGCGTG GAGGACTGTG AGGACACTCG GGAGGCAAAG 
GGGCCCTGGG GTGCACGGGT GGGCACGAGC GGCGGCAGCA CCCACGGCCG GCACACGGTG 
GGCAGCGACC CAGCCGAGCA CCCGCTCCTG CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG 
CTGAGCCGCA AGGGCTCCCG GCACACGGGG AATGCGGCAG CCGAGTGGAT CAGCCAACAG 
CGGCTCAGCC TGTACCGGCG CTCCGAGAGG CAGGAGCTCT CAGAGCTGGT CAAGAACCGC 
ATGAAGCACC TGGGGCTGCC CACCACTGGC TACGAGGATG TAGCAAATTT AACAGCCAGT 
GATGTCATGA ATCGGGTAAA CCTGGGATAT TTGCAAGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG 
AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855394.1
CDS	30..3632
Translation	MSDLDSEVLPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999626.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999626.1

ORF Insert Sequence:

ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC 
ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGT GGCCATGGAC 
CTCCAGAATA CGGAGTGCCG ACCCACACAG AGTGGCGGGG GCAGTGGGGG CAGCAAGCTG 
GCGCTCCCCA CGGAGAACGG CTCAGGCAGC CGGCGGCCCA GCATCGCGCC GGTGCTGGAG 
GTGGCTGACA GCTCAGCCCT GCTGCCCTGC GACCTGCTGA GTGACCAGTC AGAGGACGAG 
ATGACGCCGT CGGAGGATGA GGGGCTGTCC GTGGTGGAGT ATGTGAAGGG CTACCCTCCT 
AACTCACCCT ACATCGGCAG CTCCCCAACC CTGTGCCATC TCCTGCCCGT GAAAGCCCCC 
TTCTGCTGCC TGCGGCTGGA CAAGGGCTGC AAGCACAACA GCTACGAAGA TGCCAAGGCC 
TATGGCTTCA AGAACAAGCT GATCATCGTC TCAGCGGAGA CGGCGGGCAA CGGGCTGTAC 
AACTTCATCG TGCCGCTGCG CGCCTACTAC CGGCCTCGCC GGGAGCTCAA CCCCATTGTG 
CTGCTGCTGG ACAACAAGCC CGACCACCAC TTCCTGGAGG CCATCTGCTG CTTTCCCATG 
GTCTACTACA TGGAGGGCTC CGTGGACAAC CTGGACAGCC TGCTGCGGTG CGGTGTCATC 
TACGCGGACA ATCTGGTGGT GGCAGACAAG GAGAGCACCA TGAGCGCTGA GGAGGACTAC 
ATGGCCGACG CAAAGACCAT CGTCAATGTG CAGACCATGT TCCGGCTCTT CCCCAGTCTC 
AGCATCACCA CCGAGCTCAC CCACCCGTCC AACATGCGGT TCATGCAGTT TCGCGCCAAG 
GACAGCTACT CTCTGGCTCT CTCTAAACTG GAGAAGAGGG AGCGTGAGAA CGGCTCCAAC 
CTGGCCTTCA TGTTCCGCCT GCCCTTCGCC GCCGGCCGCG TCTTCAGCAT CAGCATGCTG 
GACACACTGC TGTATCAGTC CTTCGTGAAG GACTACATGA TCCCCATCAC CAGGCTGTTG 
CTGGGCTTGG ACACCACGCC GGGCTCCGGC TACCTCTGTG CGATGAAGAT CTCGGAGGAC 
GACCTGTGGA TCCGCACCTA TGGCCGCCTC TTCCAGAAGC TGTGCTCCTC CAGCGCCGAG 
ATCCCTATCG GCATCTACAG GACCCAGTGC CACGTCTTCT CCACCGAGCC CCCCGACCTC 
AGGGCCCAGT CCCAGGTCTC GGTGAGCGTG GAGGACTGTG AGGACACTCG GGAGGCAAAG 
GGGCCCTGGG GTGCACGGGT GGGCACGAGC GGCGGCAGCA CCCACGGCCG GCACACGGTG 
GGCAGCGACC CAGCCGAGCA CCCGCTCCTG CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG 
CTGAGCCGCA AGGGCTCCCG GCACACGGGG AATGCGGCAG CCGAGTGGAT CAGCCAACAG 
CGGCTCAGCC TGTACCGGCG CTCCGAGAGG CAGGAGCTCT CAGAGCTGGT CAAGAACCGC 
ATGAAGCACC TGGGGCTGCC CACCACTGGC TACGAGGATG TAGCAAATTT AACAGCCAGT 
GATGTCATGA ATCGGGTAAA CCTGGGATAT TTGCAAGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG 
AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473691.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329177.2 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa220974
Clone ID Related Accession (Same CDS sequence)	XM_015473691.2
Accession Version	XM_015473691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
ACTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329177.2
CDS	908..4486
Translation	MPGAGGCCHCLFPRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473691.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473691.2

ORF Insert Sequence:

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
ACTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999627.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855395.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X17, mRNA.

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CloneID	OBa220975
Clone ID Related Accession (Same CDS sequence)	XM_024999627.1
Accession Version	XM_024999627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTGTTCTTCA TCAAAAACCA AAGATCCAGC 
CTGCGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC 
CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATCGGATGCT GGGGCTGCCC GAAGCAGAAT 
TACACCTTCA ATGAGTCGTC CTCCGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG CGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGTGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCTTGCT GTGCCTGGTC TTCACAGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACATCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACGCCC AAGATCTGGC CGTCCCAGCT GCTGGTGGTC 
ATCATGATCT GTGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGATT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA TGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CTGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCAAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AATAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCGGTG CACAGCATCA TTGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAATAC GGAGTGCCGA CCCACACAGA GTGGCGGGGG CAGTGGGGGC 
AGCAAGCTGG CGCTCCCCAC GGAGAACGGC TCAGGCAGCC GGCGGCCCAG CATCGCGCCG 
GTGCTGGAGG TGGCTGACAG CTCAGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGATGAG GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCAACCC TGTGCCATCT CCTGCCCGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ATGGCTTCAA GAACAAGCTG ATCATCGTCT CAGCGGAGAC GGCGGGCAAC 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGTGTCATCT ACGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGCGCTGAG 
GAGGACTACA TGGCCGACGC AAAGACCATC GTCAATGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCCGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAT GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAGTGCC ACGTCTTCTC CACCGAGCCC 
CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC 
TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855395.1
CDS	208..3783
Translation	MSRPEWSLECGYRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999627.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999627.1

ORF Insert Sequence:

ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTGTTCTTCA TCAAAAACCA AAGATCCAGC 
CTGCGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC 
CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATCGGATGCT GGGGCTGCCC GAAGCAGAAT 
TACACCTTCA ATGAGTCGTC CTCCGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG CGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGTGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCTTGCT GTGCCTGGTC TTCACAGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACATCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACGCCC AAGATCTGGC CGTCCCAGCT GCTGGTGGTC 
ATCATGATCT GTGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGATT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA TGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CTGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCAAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AATAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCGGTG CACAGCATCA TTGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAATAC GGAGTGCCGA CCCACACAGA GTGGCGGGGG CAGTGGGGGC 
AGCAAGCTGG CGCTCCCCAC GGAGAACGGC TCAGGCAGCC GGCGGCCCAG CATCGCGCCG 
GTGCTGGAGG TGGCTGACAG CTCAGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGATGAG GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCAACCC TGTGCCATCT CCTGCCCGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ATGGCTTCAA GAACAAGCTG ATCATCGTCT CAGCGGAGAC GGCGGGCAAC 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGTGTCATCT ACGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGCGCTGAG 
GAGGACTACA TGGCCGACGC AAAGACCATC GTCAATGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCCGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAT GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAGTGCC ACGTCTTCTC CACCGAGCCC 
CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC 
TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999628.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855396.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa220976
Clone ID Related Accession (Same CDS sequence)	XM_024999628.1
Accession Version	XM_024999628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855396.1
CDS	1472..5011
Translation	MVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999628.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999628.1

ORF Insert Sequence:

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999629.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855397.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa220977
Clone ID Related Accession (Same CDS sequence)	XM_024999629.1
Accession Version	XM_024999629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACATC GAGGCACCTG GCTTTACATC CCCAGGGAGA CCAGCTGGGG CTGCCCGAAG 
CAGAATTACA CCTTCAATGA GTCGTCCTCC GAGATCAACT GGGCCCCCAT CCTGTGGGTG 
GAGAGGAAGG TGGCTCTGTG GGCCATTCAG GTCATCGTGG CCATCATCAG CTTCCTGGAG 
ACCATGCTTC TCATTTACCT CAGCTACAAA GGCAACATCT GGGAGCAGAT CTTCCGCGTG 
TCCTTCATCC TGGAGATGAT CAACACGCTG CCCTTCATCA TCACGATATT CTGGCCGCCG 
CTGCGGAACC TGTTCATCCC CGTGTTTCTG AACTGCTGGC TGGCCAAGCA CGCCCTGGAG 
AACATGATCA ACGACTTCCA CCGTGCCATC CTGCGCTCGC AGTCGGCCAT GTTCAATCAG 
GTCCTCATCC TCTTCTGCAC CTTGCTGTGC CTGGTCTTCA CAGGGACCTG CGGCATCCAG 
CACCTGGAGC GCGCGGGCGA CAACCTGTCC CTCCTGACAT CCTTCTACTT CTGCATCGTC 
ACCTTCTCCA CCGTGGGCTA TGGGGACGTG ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG 
GTGGTCATCA TGATCTGTGT GGCCCTGGTG GTGCTCCCGC TGCAGTTTGA GGAACTCGTC 
TACCTGTGGA TGGAGCGGCA GAAGTCGGGG GGCAACTACA GCCGCCACCG GGCCCAGACG 
GAGAAGCATG TGATCCTGTG CGTGAGCTCC CTCAAGATCG ACCTGCTCAT GGACTTTCTG 
AACGAGTTCT ACGCGCATCC ACGATTGCAG GACTACTACG TCGTCATCCT GTGCCCCACG 
GAGATGGACA TCCAGGTTCG CAGGGTCCTG CAGATCCCAC TGTGGTCCCA GCGGGTCATC 
TACCTCCAGG GCTCCGCGCT CAAGGATCAG GACCTCATGC GGGCCAAGAT GGACAATGGG 
GAGGCCTGCT TTATTCTCAG CAGCCGGAAC GAGGTGGACC GCACCGCTGC GGACCACCAG 
ACCATACTGC GTGCCTGGGC CGTGAAGGAC TTTGCCCCAA ACTGCCCCCT CTACGTCCAG 
ATCCTCAAGC CTGAGAACAA GTTTCATGTC AAGTTTGCAG AGCACGTGGT GTGCGAGGAG 
GAGTGCAAGT ATGCCATGCT GGCCCTCAAC TGCATCTGCC CAGCCACGTC CACCCTCATC 
ACCCTGCTGG TGCACACGTC CCGCGGCCAG GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG 
CGCATGTACG GACGCTGCTC GGGCAACGAG GTCTACCACG TGCGGATGGG GGACAGCAAG 
TTCTTCCGCG AGTACGAGGG CAAGAGCTTC ACCTACGCCG CCTTCCACGC GCACAAGAAG 
TACGGCGTGT GCCTCATCGG CCTGAAGCGC GAGGACAATA AGAGCATCCT GCTGAACCCG 
GGCCCACGGC ACATCCTGGC GGCCTCCGAC ACCTGCTTCT ACATCAACAT CACCAAGGAG 
GAGAACTCGG CCTTCATCTT CAAGCAGGAG GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG 
CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG CCGGTGCACA GCATCATTGC CTCCATGGGG 
ACAGTGGCCA TGGACCTCCA GAATACGGAG TGCCGACCCA CACAGAGTGG CGGGGGCAGT 
GGGGGCAGCA AGCTGGCGCT CCCCACGGAG AACGGCTCAG GCAGCCGGCG GCCCAGCATC 
GCGCCGGTGC TGGAGGTGGC TGACAGCTCA GCCCTGCTGC CCTGCGACCT GCTGAGTGAC 
CAGTCAGAGG ACGAGATGAC GCCGTCGGAG GATGAGGGGC TGTCCGTGGT GGAGTATGTG 
AAGGGCTACC CTCCTAACTC ACCCTACATC GGCAGCTCCC CAACCCTGTG CCATCTCCTG 
CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG CTGGACAAGG GCTGCAAGCA CAACAGCTAC 
GAAGATGCCA AGGCCTATGG CTTCAAGAAC AAGCTGATCA TCGTCTCAGC GGAGACGGCG 
GGCAACGGGC TGTACAACTT CATCGTGCCG CTGCGCGCCT ACTACCGGCC TCGCCGGGAG 
CTCAACCCCA TTGTGCTGCT GCTGGACAAC AAGCCCGACC ACCACTTCCT GGAGGCCATC 
TGCTGCTTTC CCATGGTCTA CTACATGGAG GGCTCCGTGG ACAACCTGGA CAGCCTGCTG 
CGGTGCGGTG TCATCTACGC GGACAATCTG GTGGTGGCAG ACAAGGAGAG CACCATGAGC 
GCTGAGGAGG ACTACATGGC CGACGCAAAG ACCATCGTCA ATGTGCAGAC CATGTTCCGG 
CTCTTCCCCA GTCTCAGCAT CACCACCGAG CTCACCCACC CGTCCAACAT GCGGTTCATG 
CAGTTTCGCG CCAAGGACAG CTACTCTCTG GCTCTCTCTA AACTGGAGAA GAGGGAGCGT 
GAGAACGGCT CCAACCTGGC CTTCATGTTC CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC 
AGCATCAGCA TGCTGGACAC ACTGCTGTAT CAGTCCTTCG TGAAGGACTA CATGATCCCC 
ATCACCAGGC TGTTGCTGGG CTTGGACACC ACGCCGGGCT CCGGCTACCT CTGTGCGATG 
AAGATCTCGG AGGACGACCT GTGGATCCGC ACCTATGGCC GCCTCTTCCA GAAGCTGTGC 
TCCTCCAGCG CCGAGATCCC TATCGGCATC TACAGGACCC AGTGCCACGT CTTCTCCACC 
GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG 
GTGAGCGTGG AGGACTGTGA GGACACTCGG GAGGCAAAGG GGCCCTGGGG TGCACGGGTG 
GGCACGAGCG GCGGCAGCAC CCACGGCCGG CACACGGTGG GCAGCGACCC AGCCGAGCAC 
CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG 
CACACGGGGA ATGCGGCAGC CGAGTGGATC AGCCAACAGC GGCTCAGCCT GTACCGGCGC 
TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC AAGAACCGCA TGAAGCACCT GGGGCTGCCC 
ACCACTGGCT ACGAGGATGT AGCAAATTTA ACAGCCAGTG ATGTCATGAA TCGGGTAAAC 
CTGGGATATT TGCAAGACGA GATGAATGAC CACCAGAACA CCCTCTCCTA TGTGCTCATC 
AACCCCCCGC CCGACACGCG GCTGGAGCCC AATGACATTG TATATCTCAT CCGCTCCGAC 
CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG AGCCGGAAGA GCAGCTGCAG CAACAAGCTG 
GCCTCCTGCA ACCCTGAGAC TCGGGACGAG ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855397.1
CDS	139..3540
Translation	MRHRGTWLYIPRETSWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999629.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999629.1

ORF Insert Sequence:

ATGAGACATC GAGGCACCTG GCTTTACATC CCCAGGGAGA CCAGCTGGGG CTGCCCGAAG 
CAGAATTACA CCTTCAATGA GTCGTCCTCC GAGATCAACT GGGCCCCCAT CCTGTGGGTG 
GAGAGGAAGG TGGCTCTGTG GGCCATTCAG GTCATCGTGG CCATCATCAG CTTCCTGGAG 
ACCATGCTTC TCATTTACCT CAGCTACAAA GGCAACATCT GGGAGCAGAT CTTCCGCGTG 
TCCTTCATCC TGGAGATGAT CAACACGCTG CCCTTCATCA TCACGATATT CTGGCCGCCG 
CTGCGGAACC TGTTCATCCC CGTGTTTCTG AACTGCTGGC TGGCCAAGCA CGCCCTGGAG 
AACATGATCA ACGACTTCCA CCGTGCCATC CTGCGCTCGC AGTCGGCCAT GTTCAATCAG 
GTCCTCATCC TCTTCTGCAC CTTGCTGTGC CTGGTCTTCA CAGGGACCTG CGGCATCCAG 
CACCTGGAGC GCGCGGGCGA CAACCTGTCC CTCCTGACAT CCTTCTACTT CTGCATCGTC 
ACCTTCTCCA CCGTGGGCTA TGGGGACGTG ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG 
GTGGTCATCA TGATCTGTGT GGCCCTGGTG GTGCTCCCGC TGCAGTTTGA GGAACTCGTC 
TACCTGTGGA TGGAGCGGCA GAAGTCGGGG GGCAACTACA GCCGCCACCG GGCCCAGACG 
GAGAAGCATG TGATCCTGTG CGTGAGCTCC CTCAAGATCG ACCTGCTCAT GGACTTTCTG 
AACGAGTTCT ACGCGCATCC ACGATTGCAG GACTACTACG TCGTCATCCT GTGCCCCACG 
GAGATGGACA TCCAGGTTCG CAGGGTCCTG CAGATCCCAC TGTGGTCCCA GCGGGTCATC 
TACCTCCAGG GCTCCGCGCT CAAGGATCAG GACCTCATGC GGGCCAAGAT GGACAATGGG 
GAGGCCTGCT TTATTCTCAG CAGCCGGAAC GAGGTGGACC GCACCGCTGC GGACCACCAG 
ACCATACTGC GTGCCTGGGC CGTGAAGGAC TTTGCCCCAA ACTGCCCCCT CTACGTCCAG 
ATCCTCAAGC CTGAGAACAA GTTTCATGTC AAGTTTGCAG AGCACGTGGT GTGCGAGGAG 
GAGTGCAAGT ATGCCATGCT GGCCCTCAAC TGCATCTGCC CAGCCACGTC CACCCTCATC 
ACCCTGCTGG TGCACACGTC CCGCGGCCAG GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG 
CGCATGTACG GACGCTGCTC GGGCAACGAG GTCTACCACG TGCGGATGGG GGACAGCAAG 
TTCTTCCGCG AGTACGAGGG CAAGAGCTTC ACCTACGCCG CCTTCCACGC GCACAAGAAG 
TACGGCGTGT GCCTCATCGG CCTGAAGCGC GAGGACAATA AGAGCATCCT GCTGAACCCG 
GGCCCACGGC ACATCCTGGC GGCCTCCGAC ACCTGCTTCT ACATCAACAT CACCAAGGAG 
GAGAACTCGG CCTTCATCTT CAAGCAGGAG GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG 
CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG CCGGTGCACA GCATCATTGC CTCCATGGGG 
ACAGTGGCCA TGGACCTCCA GAATACGGAG TGCCGACCCA CACAGAGTGG CGGGGGCAGT 
GGGGGCAGCA AGCTGGCGCT CCCCACGGAG AACGGCTCAG GCAGCCGGCG GCCCAGCATC 
GCGCCGGTGC TGGAGGTGGC TGACAGCTCA GCCCTGCTGC CCTGCGACCT GCTGAGTGAC 
CAGTCAGAGG ACGAGATGAC GCCGTCGGAG GATGAGGGGC TGTCCGTGGT GGAGTATGTG 
AAGGGCTACC CTCCTAACTC ACCCTACATC GGCAGCTCCC CAACCCTGTG CCATCTCCTG 
CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG CTGGACAAGG GCTGCAAGCA CAACAGCTAC 
GAAGATGCCA AGGCCTATGG CTTCAAGAAC AAGCTGATCA TCGTCTCAGC GGAGACGGCG 
GGCAACGGGC TGTACAACTT CATCGTGCCG CTGCGCGCCT ACTACCGGCC TCGCCGGGAG 
CTCAACCCCA TTGTGCTGCT GCTGGACAAC AAGCCCGACC ACCACTTCCT GGAGGCCATC 
TGCTGCTTTC CCATGGTCTA CTACATGGAG GGCTCCGTGG ACAACCTGGA CAGCCTGCTG 
CGGTGCGGTG TCATCTACGC GGACAATCTG GTGGTGGCAG ACAAGGAGAG CACCATGAGC 
GCTGAGGAGG ACTACATGGC CGACGCAAAG ACCATCGTCA ATGTGCAGAC CATGTTCCGG 
CTCTTCCCCA GTCTCAGCAT CACCACCGAG CTCACCCACC CGTCCAACAT GCGGTTCATG 
CAGTTTCGCG CCAAGGACAG CTACTCTCTG GCTCTCTCTA AACTGGAGAA GAGGGAGCGT 
GAGAACGGCT CCAACCTGGC CTTCATGTTC CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC 
AGCATCAGCA TGCTGGACAC ACTGCTGTAT CAGTCCTTCG TGAAGGACTA CATGATCCCC 
ATCACCAGGC TGTTGCTGGG CTTGGACACC ACGCCGGGCT CCGGCTACCT CTGTGCGATG 
AAGATCTCGG AGGACGACCT GTGGATCCGC ACCTATGGCC GCCTCTTCCA GAAGCTGTGC 
TCCTCCAGCG CCGAGATCCC TATCGGCATC TACAGGACCC AGTGCCACGT CTTCTCCACC 
GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG 
GTGAGCGTGG AGGACTGTGA GGACACTCGG GAGGCAAAGG GGCCCTGGGG TGCACGGGTG 
GGCACGAGCG GCGGCAGCAC CCACGGCCGG CACACGGTGG GCAGCGACCC AGCCGAGCAC 
CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG 
CACACGGGGA ATGCGGCAGC CGAGTGGATC AGCCAACAGC GGCTCAGCCT GTACCGGCGC 
TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC AAGAACCGCA TGAAGCACCT GGGGCTGCCC 
ACCACTGGCT ACGAGGATGT AGCAAATTTA ACAGCCAGTG ATGTCATGAA TCGGGTAAAC 
CTGGGATATT TGCAAGACGA GATGAATGAC CACCAGAACA CCCTCTCCTA TGTGCTCATC 
AACCCCCCGC CCGACACGCG GCTGGAGCCC AATGACATTG TATATCTCAT CCGCTCCGAC 
CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG AGCCGGAAGA GCAGCTGCAG CAACAAGCTG 
GCCTCCTGCA ACCCTGAGAC TCGGGACGAG ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999630.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855398.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X20, mRNA.

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CloneID	OBa220978
Clone ID Related Accession (Same CDS sequence)	XM_024999630.1
Accession Version	XM_024999630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GACTCAGGTT CCTGGTGGCA TCATCGTTTC AATATCCGCG 
GCACCTGTGA CAGTGACGTC GACCAGGGCC CAGCCTGCCC GTTCAGTTCT GCTCCCTCCA 
GACACGTGGT TCTCAGAAGG GCTCGTCCAC GTGGGTTCCC ATGTCTCGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855398.1
CDS	659..3892
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKTQVPGGIIVSISAAPVTVTSTRAQPARSVLLPPDTWFSEGLVHVGSHVSL

Target ORF information:

RefSeq Version	XM_024999630.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999630.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GACTCAGGTT CCTGGTGGCA TCATCGTTTC AATATCCGCG 
GCACCTGTGA CAGTGACGTC GACCAGGGCC CAGCCTGCCC GTTCAGTTCT GCTCCCTCCA 
GACACGTGGT TCTCAGAAGG GCTCGTCCAC GTGGGTTCCC ATGTCTCGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_024999631.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855399.1 Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa220979
Clone ID Related Accession (Same CDS sequence)	XM_024999631.1
Accession Version	XM_024999631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTTC ACGACGGGGT GCGCAGGGGT CCAGGAGATG TCTACAGCGG GGTGCAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCAGCCC 
GGCGCCGAGG ACGGCGGCCG CGGGGACGGC GCGCGCCTGG ACACCGCCGG CCTCAAGATG 
AGCGACCTGG ACTCGGAGGT GCTGCCCTTG CCTCCGCGGT ACCGCTTCCG GGACCTGCTG 
CTGGGCGACC AGTCCTTCCA GAACGACGAC AGGGTCCAGG TGGAGTTCTA CGTCAACGAG 
AACACCTTCA AGGAGAGGCT TAAGCTGTTC TTCATCAAAA ACCAAAGATC CAGCCTGCGG 
ATCCGGCTGT TCAACTTCTC CCTGAAGCTG CTGACCTGCC TGCTGTACAT TGTCCGCGTC 
CTGCTGGACG ACCCAGCCCT GGGGATCGGA TGCTGGGGCT GCCCGAAGCA GAATTACACC 
TTCAATGAGT CGTCCTCCGA GATCAACTGG GCCCCCATCC TGTGGGTGGA GAGGAAGGTG 
GCTCTGTGGG CCATTCAGGT CATCGTGGCC ATCATCAGCT TCCTGGAGAC CATGCTTCTC 
ATTTACCTCA GCTACAAAGG CAACATCTGG GAGCAGATCT TCCGCGTGTC CTTCATCCTG 
GAGATGATCA ACACGCTGCC CTTCATCATC ACGATATTCT GGCCGCCGCT GCGGAACCTG 
TTCATCCCCG TGTTTCTGAA CTGCTGGCTG GCCAAGCACG CCCTGGAGAA CATGATCAAC 
GACTTCCACC GTGCCATCCT GCGCTCGCAG TCGGCCATGT TCAATCAGGT CCTCATCCTC 
TTCTGCACCT TGCTGTGCCT GGTCTTCACA GGGACCTGCG GCATCCAGCA CCTGGAGCGC 
GCGGGCGACA ACCTGTCCCT CCTGACATCC TTCTACTTCT GCATCGTCAC CTTCTCCACC 
GTGGGCTATG GGGACGTGAC GCCCAAGATC TGGCCGTCCC AGCTGCTGGT GGTCATCATG 
ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG CAGTTTGAGG AACTCGTCTA CCTGTGGATG 
GAGCGGCAGA AGTCGGGGGG CAACTACAGC CGCCACCGGG CCCAGACGGA GAAGCATGTG 
ATCCTGTGCG TGAGCTCCCT CAAGATCGAC CTGCTCATGG ACTTTCTGAA CGAGTTCTAC 
GCGCATCCAC GATTGCAGGA CTACTACGTC GTCATCCTGT GCCCCACGGA GATGGACATC 
CAGGTTCGCA GGGTCCTGCA GATCCCACTG TGGTCCCAGC GGGTCATCTA CCTCCAGGGC 
TCCGCGCTCA AGGATCAGGA CCTCATGCGG GCCAAGATGG ACAATGGGGA GGCCTGCTTT 
ATTCTCAGCA GCCGGAACGA GGTGGACCGC ACCGCTGCGG ACCACCAGAC CATACTGCGT 
GCCTGGGCCG TGAAGGACTT TGCCCCAAAC TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT 
GAGAACAAGT TTCATGTCAA GTTTGCAGAG CACGTGGTGT GCGAGGAGGA GTGCAAGTAT 
GCCATGCTGG CCCTCAACTG CATCTGCCCA GCCACGTCCA CCCTCATCAC CCTGCTGGTG 
CACACGTCCC GCGGCCAGGA AGGCCAGGAC TCGCCAGAGC AGTGGCAGCG CATGTACGGA 
CGCTGCTCGG GCAACGAGGT CTACCACGTG CGGATGGGGG ACAGCAAGTT CTTCCGCGAG 
TACGAGGGCA AGAGCTTCAC CTACGCCGCC TTCCACGCGC ACAAGAAGTA CGGCGTGTGC 
CTCATCGGCC TGAAGCGCGA GGACAATAAG AGCATCCTGC TGAACCCGGG CCCACGGCAC 
ATCCTGGCGG CCTCCGACAC CTGCTTCTAC ATCAACATCA CCAAGGAGGA GAACTCGGCC 
TTCATCTTCA AGCAGGAGGA AAAGCAGAAG AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC 
GAGGGCTCAT CCCGCCTGCC GGTGCACAGC ATCATTGCCT CCATGGGGAC AGTGGCCATG 
GACCTCCAGA ATACGGAGTG CCGACCCACA CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG 
CTGGCGCTCC CCACGGAGAA CGGCTCAGGC AGCCGGCGGC CCAGCATCGC GCCGGTGCTG 
GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAC 
GAGATGACGC CGTCGGAGGA TGAGGGGCTG TCCGTGGTGG AGTATGTGAA GGGCTACCCT 
CCTAACTCAC CCTACATCGG CAGCTCCCCA ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC 
CCCTTCTGCT GCCTGCGGCT GGACAAGGGC TGCAAGCACA ACAGCTACGA AGATGCCAAG 
GCCTATGGCT TCAAGAACAA GCTGATCATC GTCTCAGCGG AGACGGCGGG CAACGGGCTG 
TACAACTTCA TCGTGCCGCT GCGCGCCTAC TACCGGCCTC GCCGGGAGCT CAACCCCATT 
GTGCTGCTGC TGGACAACAA GCCCGACCAC CACTTCCTGG AGGCCATCTG CTGCTTTCCC 
ATGGTCTACT ACATGGAGGG CTCCGTGGAC AACCTGGACA GCCTGCTGCG GTGCGGTGTC 
ATCTACGCGG ACAATCTGGT GGTGGCAGAC AAGGAGAGCA CCATGAGCGC TGAGGAGGAC 
TACATGGCCG ACGCAAAGAC CATCGTCAAT GTGCAGACCA TGTTCCGGCT CTTCCCCAGT 
CTCAGCATCA CCACCGAGCT CACCCACCCG TCCAACATGC GGTTCATGCA GTTTCGCGCC 
AAGGACAGCT ACTCTCTGGC TCTCTCTAAA CTGGAGAAGA GGGAGCGTGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCCTTC GCCGCCGGCC GCGTCTTCAG CATCAGCATG 
CTGGACACAC TGCTGTATCA GTCCTTCGTG AAGGACTACA TGATCCCCAT CACCAGGCTG 
TTGCTGGGCT TGGACACCAC GCCGGGCTCC GGCTACCTCT GTGCGATGAA GATCTCGGAG 
GACGACCTGT GGATCCGCAC CTATGGCCGC CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC 
GAGATCCCTA TCGGCATCTA CAGGACCCAG TGCCACGTCT TCTCCACCGA GCCCCCCGAC 
CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG 
AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855399.1
CDS	25..3747
Translation	MPLHDGVRRGPGDVYSGVQGGGHTNRTFVFDDGQCAPRQPGAEDGGRGDGARLDTAGLKMSDLDSEVLPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_024999631.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999631.1

ORF Insert Sequence:

ATGCCGCTTC ACGACGGGGT GCGCAGGGGT CCAGGAGATG TCTACAGCGG GGTGCAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCAGCCC 
GGCGCCGAGG ACGGCGGCCG CGGGGACGGC GCGCGCCTGG ACACCGCCGG CCTCAAGATG 
AGCGACCTGG ACTCGGAGGT GCTGCCCTTG CCTCCGCGGT ACCGCTTCCG GGACCTGCTG 
CTGGGCGACC AGTCCTTCCA GAACGACGAC AGGGTCCAGG TGGAGTTCTA CGTCAACGAG 
AACACCTTCA AGGAGAGGCT TAAGCTGTTC TTCATCAAAA ACCAAAGATC CAGCCTGCGG 
ATCCGGCTGT TCAACTTCTC CCTGAAGCTG CTGACCTGCC TGCTGTACAT TGTCCGCGTC 
CTGCTGGACG ACCCAGCCCT GGGGATCGGA TGCTGGGGCT GCCCGAAGCA GAATTACACC 
TTCAATGAGT CGTCCTCCGA GATCAACTGG GCCCCCATCC TGTGGGTGGA GAGGAAGGTG 
GCTCTGTGGG CCATTCAGGT CATCGTGGCC ATCATCAGCT TCCTGGAGAC CATGCTTCTC 
ATTTACCTCA GCTACAAAGG CAACATCTGG GAGCAGATCT TCCGCGTGTC CTTCATCCTG 
GAGATGATCA ACACGCTGCC CTTCATCATC ACGATATTCT GGCCGCCGCT GCGGAACCTG 
TTCATCCCCG TGTTTCTGAA CTGCTGGCTG GCCAAGCACG CCCTGGAGAA CATGATCAAC 
GACTTCCACC GTGCCATCCT GCGCTCGCAG TCGGCCATGT TCAATCAGGT CCTCATCCTC 
TTCTGCACCT TGCTGTGCCT GGTCTTCACA GGGACCTGCG GCATCCAGCA CCTGGAGCGC 
GCGGGCGACA ACCTGTCCCT CCTGACATCC TTCTACTTCT GCATCGTCAC CTTCTCCACC 
GTGGGCTATG GGGACGTGAC GCCCAAGATC TGGCCGTCCC AGCTGCTGGT GGTCATCATG 
ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG CAGTTTGAGG AACTCGTCTA CCTGTGGATG 
GAGCGGCAGA AGTCGGGGGG CAACTACAGC CGCCACCGGG CCCAGACGGA GAAGCATGTG 
ATCCTGTGCG TGAGCTCCCT CAAGATCGAC CTGCTCATGG ACTTTCTGAA CGAGTTCTAC 
GCGCATCCAC GATTGCAGGA CTACTACGTC GTCATCCTGT GCCCCACGGA GATGGACATC 
CAGGTTCGCA GGGTCCTGCA GATCCCACTG TGGTCCCAGC GGGTCATCTA CCTCCAGGGC 
TCCGCGCTCA AGGATCAGGA CCTCATGCGG GCCAAGATGG ACAATGGGGA GGCCTGCTTT 
ATTCTCAGCA GCCGGAACGA GGTGGACCGC ACCGCTGCGG ACCACCAGAC CATACTGCGT 
GCCTGGGCCG TGAAGGACTT TGCCCCAAAC TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT 
GAGAACAAGT TTCATGTCAA GTTTGCAGAG CACGTGGTGT GCGAGGAGGA GTGCAAGTAT 
GCCATGCTGG CCCTCAACTG CATCTGCCCA GCCACGTCCA CCCTCATCAC CCTGCTGGTG 
CACACGTCCC GCGGCCAGGA AGGCCAGGAC TCGCCAGAGC AGTGGCAGCG CATGTACGGA 
CGCTGCTCGG GCAACGAGGT CTACCACGTG CGGATGGGGG ACAGCAAGTT CTTCCGCGAG 
TACGAGGGCA AGAGCTTCAC CTACGCCGCC TTCCACGCGC ACAAGAAGTA CGGCGTGTGC 
CTCATCGGCC TGAAGCGCGA GGACAATAAG AGCATCCTGC TGAACCCGGG CCCACGGCAC 
ATCCTGGCGG CCTCCGACAC CTGCTTCTAC ATCAACATCA CCAAGGAGGA GAACTCGGCC 
TTCATCTTCA AGCAGGAGGA AAAGCAGAAG AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC 
GAGGGCTCAT CCCGCCTGCC GGTGCACAGC ATCATTGCCT CCATGGGGAC AGTGGCCATG 
GACCTCCAGA ATACGGAGTG CCGACCCACA CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG 
CTGGCGCTCC CCACGGAGAA CGGCTCAGGC AGCCGGCGGC CCAGCATCGC GCCGGTGCTG 
GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAC 
GAGATGACGC CGTCGGAGGA TGAGGGGCTG TCCGTGGTGG AGTATGTGAA GGGCTACCCT 
CCTAACTCAC CCTACATCGG CAGCTCCCCA ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC 
CCCTTCTGCT GCCTGCGGCT GGACAAGGGC TGCAAGCACA ACAGCTACGA AGATGCCAAG 
GCCTATGGCT TCAAGAACAA GCTGATCATC GTCTCAGCGG AGACGGCGGG CAACGGGCTG 
TACAACTTCA TCGTGCCGCT GCGCGCCTAC TACCGGCCTC GCCGGGAGCT CAACCCCATT 
GTGCTGCTGC TGGACAACAA GCCCGACCAC CACTTCCTGG AGGCCATCTG CTGCTTTCCC 
ATGGTCTACT ACATGGAGGG CTCCGTGGAC AACCTGGACA GCCTGCTGCG GTGCGGTGTC 
ATCTACGCGG ACAATCTGGT GGTGGCAGAC AAGGAGAGCA CCATGAGCGC TGAGGAGGAC 
TACATGGCCG ACGCAAAGAC CATCGTCAAT GTGCAGACCA TGTTCCGGCT CTTCCCCAGT 
CTCAGCATCA CCACCGAGCT CACCCACCCG TCCAACATGC GGTTCATGCA GTTTCGCGCC 
AAGGACAGCT ACTCTCTGGC TCTCTCTAAA CTGGAGAAGA GGGAGCGTGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCCTTC GCCGCCGGCC GCGTCTTCAG CATCAGCATG 
CTGGACACAC TGCTGTATCA GTCCTTCGTG AAGGACTACA TGATCCCCAT CACCAGGCTG 
TTGCTGGGCT TGGACACCAC GCCGGGCTCC GGCTACCTCT GTGCGATGAA GATCTCGGAG 
GACGACCTGT GGATCCGCAC CTATGGCCGC CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC 
GAGATCCCTA TCGGCATCTA CAGGACCCAG TGCCACGTCT TCTCCACCGA GCCCCCCGAC 
CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG 
AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015465523.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321009.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92023
Clone ID Related Accession (Same CDS sequence)	XM_015465523.1 , XM_015473687.1
Accession Version	XM_015465523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC 
CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC 
AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG 
AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG 
ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321009.1
CDS	104..4135
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015465523.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465523.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC 
CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC 
AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG 
AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG 
ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473687.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329173.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

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CloneID	OBa92023
Clone ID Related Accession (Same CDS sequence)	XM_015465523.1 , XM_015473687.1
Accession Version	XM_015473687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC 
CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC 
AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG 
AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG 
ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329173.1
CDS	104..4135
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473687.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473687.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC 
CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC 
AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG 
AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG 
ACACAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473688.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329174.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92024
Clone ID Related Accession (Same CDS sequence)	XM_015473688.1 , XM_015465524.1
Accession Version	XM_015473688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA 
CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC 
TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG 
CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG 
GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC 
TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC 
AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG 
ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC 
GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC 
GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC 
GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG 
GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC 
GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC 
CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC 
TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC 
TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG 
GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT 
GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG 
ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC 
CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC 
AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG 
AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC 
ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG 
AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329174.1
CDS	104..4129
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473688.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473688.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA 
CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC 
TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG 
CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG 
GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC 
TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC 
AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG 
ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC 
GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC 
GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC 
GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG 
GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC 
GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC 
CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC 
TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC 
TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG 
GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT 
GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG 
ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC 
CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC 
AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG 
AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC 
ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG 
AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015465524.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321010.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92024
Clone ID Related Accession (Same CDS sequence)	XM_015473688.1 , XM_015465524.1
Accession Version	XM_015465524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA 
CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC 
TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG 
CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG 
GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC 
TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC 
AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG 
ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC 
GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC 
GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC 
GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG 
GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC 
GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC 
CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC 
TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC 
TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG 
GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT 
GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG 
ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC 
CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC 
AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG 
AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC 
ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG 
AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321010.1
CDS	104..4129
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015465524.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465524.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA 
CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC 
TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG 
CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG 
GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC 
TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC 
AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG 
ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC 
GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC 
GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC 
GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG 
GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC 
GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC 
CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC 
TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG 
CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC 
TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG 
GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT 
GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG 
ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC 
CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC 
AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG 
AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC 
ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG 
AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_010819338.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817640.2 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

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CloneID	OBa92025
Clone ID Related Accession (Same CDS sequence)	XM_010819338.2 , XM_010810537.2
Accession Version	XM_010819338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC 
TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817640.2
CDS	82..3837
Translation	MYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_010819338.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819338.2

ORF Insert Sequence:

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC 
TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_010810537.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808839.2 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa92025
Clone ID Related Accession (Same CDS sequence)	XM_010819338.2 , XM_010810537.2
Accession Version	XM_010810537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC 
TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808839.2
CDS	82..3837
Translation	MYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_010810537.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810537.2

ORF Insert Sequence:

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC 
TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015465525.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321011.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa92026
Clone ID Related Accession (Same CDS sequence)	XM_015465525.1 , XM_015473689.1
Accession Version	XM_015465525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321011.1
CDS	104..4072
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015465525.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465525.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473689.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329175.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

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CloneID	OBa92026
Clone ID Related Accession (Same CDS sequence)	XM_015465525.1 , XM_015473689.1
Accession Version	XM_015473689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329175.1
CDS	104..4072
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473689.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473689.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC 
CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_010810539.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808841.2 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa92027
Clone ID Related Accession (Same CDS sequence)	XM_010810539.2 , XM_010819346.2
Accession Version	XM_010810539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808841.2
CDS	82..3774
Translation	MYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_010810539.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810539.2

ORF Insert Sequence:

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_010819346.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817648.2 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa92027
Clone ID Related Accession (Same CDS sequence)	XM_010810539.2 , XM_010819346.2
Accession Version	XM_010819346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817648.2
CDS	82..3774
Translation	MYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_010819346.2
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819346.2

ORF Insert Sequence:

ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT 
CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG 
AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT 
GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG 
CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC 
CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC 
AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015465526.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321012.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.

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CloneID	OBa92028
Clone ID Related Accession (Same CDS sequence)	XM_015465526.1 , XM_015473690.1
Accession Version	XM_015465526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA 
GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT 
ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321012.1
CDS	104..4042
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQGSHWPAPGSPPSSQEAPAWSTPGQVTRAPGMVEHSET

Target ORF information:

RefSeq Version	XM_015465526.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465526.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA 
GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT 
ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473690.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329176.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa92028
Clone ID Related Accession (Same CDS sequence)	XM_015465526.1 , XM_015473690.1
Accession Version	XM_015473690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA 
GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT 
ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329176.1
CDS	104..4042
Translation	MSSWWPLCVGCLSLPSASWVSRGAHLLLPGPWGHTRPGAVSVQSRAVGAPEPAVQPLAWLQAGARPACHLLESSPCPTGVGTGCMYPHCRGRCAASGASAGQAPPGGVRMGVPCSPRPLPESSLCVREPRLSHLSIRPLSVLMCPLHSALRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQGSHWPAPGSPPSSQEAPAWSTPGQVTRAPGMVEHSET

Target ORF information:

RefSeq Version	XM_015473690.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473690.1

ORF Insert Sequence:

ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC 
TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG 
CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG 
GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG 
GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC 
GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT 
GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC 
TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG 
CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC 
TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC 
CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC 
GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG 
GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA 
GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG 
CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG 
AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC 
CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC 
CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC 
CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG 
ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG 
GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG 
GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC 
CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG 
GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG 
CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG 
GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC 
GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC 
TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC 
AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC 
TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG 
GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG 
GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC 
ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC 
GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC 
ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG 
GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG 
CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG 
TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG 
AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA 
GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG 
GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC 
GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG 
CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC 
AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG 
CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC 
AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG 
GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG 
GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG 
ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG 
CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG 
GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC 
CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT 
CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC 
ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC 
ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC 
TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA 
CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG 
GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG 
CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG 
GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC 
AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC 
AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA 
ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA 
GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT 
ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015465528.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321014.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa92029
Clone ID Related Accession (Same CDS sequence)	XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
Accession Version	XM_015465528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321014.1
CDS	147..3725
Translation	MPGAGGCCHCLFPRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015465528.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465528.1

ORF Insert Sequence:

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473691.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329177.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa92029
Clone ID Related Accession (Same CDS sequence)	XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
Accession Version	XM_015473691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329177.1
CDS	119..3697
Translation	MPGAGGCCHCLFPRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473691.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473691.1

ORF Insert Sequence:

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015465527.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321013.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa92029
Clone ID Related Accession (Same CDS sequence)	XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
Accession Version	XM_015465527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321013.1
CDS	119..3697
Translation	MPGAGGCCHCLFPRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015465527.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465527.1

ORF Insert Sequence:

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473692.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329178.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.

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CloneID	OBa92029
Clone ID Related Accession (Same CDS sequence)	XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
Accession Version	XM_015473692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329178.1
CDS	147..3725
Translation	MPGAGGCCHCLFPRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473692.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473692.1

ORF Insert Sequence:

ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC 
AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT 
GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG 
AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG 
AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC 
ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC 
TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG 
CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC 
ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC 
CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC 
CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC 
TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG 
GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC 
CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG 
AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC 
GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG 
ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC 
CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG 
GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC 
ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC 
CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG 
TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC 
CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC 
ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC 
TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC 
GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC 
CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG 
AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG 
GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA 
GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG 
GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG 
CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG 
TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG 
GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC 
GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA 
GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC 
AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC 
AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC 
TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG 
TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT 
GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC 
TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG 
TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG 
AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC 
ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC 
ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG 
ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC 
TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG 
CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG 
AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC 
ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG 
CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC 
ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC 
GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC 
AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG 
GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC 
CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC 
TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC 
TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015465529.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321015.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa92030
Clone ID Related Accession (Same CDS sequence)	XM_015465529.1 , XM_015473693.1
Accession Version	XM_015465529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321015.1
CDS	1588..5127
Translation	MVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015465529.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465529.1

ORF Insert Sequence:

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_015473693.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329179.1 Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa92030
Clone ID Related Accession (Same CDS sequence)	XM_015465529.1 , XM_015473693.1
Accession Version	XM_015473693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	potassium channel subfamily T member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329179.1
CDS	1588..5127
Translation	MVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGNAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTSGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_015473693.1
Organism	Bos taurus(cattle)
Definition	Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473693.1

ORF Insert Sequence:

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC 
TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC 
ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA 
CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC 
AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG 
TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA 
ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC 
GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KCNT1
Entrez Gene ID	529468
Full Name	potassium sodium-activated channel subfamily T member 1
General protein information	Preferred Names potassium sodium-activated channel subfamily T member 1 Names potassium channel subfamily T member 1 potassium channel, subfamily T, member 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	KCNT1	NP_065873.2
Caenorhabditis elegans (roundworm)	slo-2	NP_001129924.1
Xenopus tropicalis (tropical clawed frog)	kcnt2	NP_001163984.2
	LOC721830	XP_001118025.2
Mus musculus (house mouse)	Kcnt1	NP_780671.2
Pan troglodytes (chimpanzee)	KCNT1	XP_003312453.2
Canis lupus familiaris (dog)	KCNT1	XP_005625191.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007585	XP_001687885.2
Drosophila melanogaster (fruit fly)	CG42732	NP_001097259.2
Bos taurus (cattle)	KCNT1	XP_002691660.3
Rattus norvegicus (Norway rat)	Kcnt1	NP_068625.1
Gallus gallus (chicken)	KCNT1	NP_989893.1
Danio rerio (zebrafish)	kcnt1	XP_001920632.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KCNT1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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