KCNT1 cDNA ORF clone, Bos taurus(cattle)

The following KCNT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220961 XM_024999617.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220962 XM_015473688.2
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220963 XM_024999618.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220964 XM_015473689.2
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220965 XM_015473690.2
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220966 XM_024999619.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220967 XM_024999620.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220968 XM_024999621.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220969 XM_024999622.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220970 XM_024999623.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220971 XM_024999624.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220972 XM_024999625.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220973 XM_024999626.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220974 XM_015473691.2
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220975 XM_024999627.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220976 XM_024999628.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220977 XM_024999629.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220978 XM_024999630.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa220979 XM_024999631.1
Latest version!
Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa92023 XM_015473687.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92023 XM_015465523.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92024 XM_015473688.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92024 XM_015465524.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92025 XM_010819338.2
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92025 XM_010810537.2
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92026 XM_015473689.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92026 XM_015465525.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92027 XM_010810539.2
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92027 XM_010819346.2
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92028 XM_015473690.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92028 XM_015465526.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92029 XM_015473692.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92029 XM_015473691.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92029 XM_015465527.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92029 XM_015465528.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92030 XM_015473693.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92030 XM_015465529.1
Latest version!
Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220961
    Clone ID Related Accession (Same CDS sequence) XM_024999617.1
    Accession Version XM_024999617.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC
    CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC
    AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG
    AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG
    ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855385.1
    CDS658..4689
    Translation

    Target ORF information:

    RefSeq Version XM_024999617.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999617.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC
    CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC
    AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG
    AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG
    ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220962
    Clone ID Related Accession (Same CDS sequence) XM_015473688.2
    Accession Version XM_015473688.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4026bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA
    CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC
    TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG
    CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG
    GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC
    TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC
    AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG
    ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC
    GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC
    GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC
    GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG
    GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC
    GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC
    CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC
    TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG
    CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC
    TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG
    GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT
    GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG
    ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC
    CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGAGG ATGTAGCAAA TTTAACAGCC
    AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG
    AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC
    ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG
    AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329174.2
    CDS658..4683
    Translation

    Target ORF information:

    RefSeq Version XM_015473688.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473688.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA
    CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC
    TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG
    CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG
    GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC
    TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC
    AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG
    ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC
    GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC
    GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC
    GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG
    GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC
    GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC
    CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC
    TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG
    CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC
    TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG
    GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT
    GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG
    ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC
    CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGAGG ATGTAGCAAA TTTAACAGCC
    AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG
    AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC
    ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG
    AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220963
    Clone ID Related Accession (Same CDS sequence) XM_024999618.1
    Accession Version XM_024999618.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGTCCCAG
    GTCTCGGTGA GCGTGGAGGA CTGTGAGGAC ACTCGGGAGG CAAAGGGGCC CTGGGGTGCA
    CGGGTGGGCA CGAGCGGCGG CAGCACCCAC GGCCGGCACA CGGTGGGCAG CGACCCAGCC
    GAGCACCCGC TCCTGCGGCG CAAGAGCCTC CCGTGGGCGC GGAGGCTGAG CCGCAAGGGC
    TCCCGGCACA CGGGGAATGC GGCAGCCGAG TGGATCAGCC AACAGCGGCT CAGCCTGTAC
    CGGCGCTCCG AGAGGCAGGA GCTCTCAGAG CTGGTCAAGA ACCGCATGAA GCACCTGGGG
    CTGCCCACCA CTGGCTACGA GGATGTAGCA AATTTAACAG CCAGTGATGT CATGAATCGG
    GTAAACCTGG GATATTTGCA AGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG
    CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC
    TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC
    AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855386.1
    CDS656..4663
    Translation

    Target ORF information:

    RefSeq Version XM_024999618.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999618.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGTCCCAG
    GTCTCGGTGA GCGTGGAGGA CTGTGAGGAC ACTCGGGAGG CAAAGGGGCC CTGGGGTGCA
    CGGGTGGGCA CGAGCGGCGG CAGCACCCAC GGCCGGCACA CGGTGGGCAG CGACCCAGCC
    GAGCACCCGC TCCTGCGGCG CAAGAGCCTC CCGTGGGCGC GGAGGCTGAG CCGCAAGGGC
    TCCCGGCACA CGGGGAATGC GGCAGCCGAG TGGATCAGCC AACAGCGGCT CAGCCTGTAC
    CGGCGCTCCG AGAGGCAGGA GCTCTCAGAG CTGGTCAAGA ACCGCATGAA GCACCTGGGG
    CTGCCCACCA CTGGCTACGA GGATGTAGCA AATTTAACAG CCAGTGATGT CATGAATCGG
    GTAAACCTGG GATATTTGCA AGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG
    CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC
    TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC
    AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220964
    Clone ID Related Accession (Same CDS sequence) XM_015473689.2
    Accession Version XM_015473689.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329175.2
    CDS657..4625
    Translation

    Target ORF information:

    RefSeq Version XM_015473689.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473689.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220965
    Clone ID Related Accession (Same CDS sequence) XM_015473690.2
    Accession Version XM_015473690.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA
    GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT
    ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329176.2
    CDS660..4598
    Translation

    Target ORF information:

    RefSeq Version XM_015473690.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473690.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCACTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA
    GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT
    ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220966
    Clone ID Related Accession (Same CDS sequence) XM_024999619.1
    Accession Version XM_024999619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG
    GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG
    GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC
    TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG
    GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC
    ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG
    CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG
    AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG
    CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC
    AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG
    GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT
    CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA
    AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC
    GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT
    CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG
    TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG
    TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC
    ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC
    CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC
    ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGGCC
    TCACCCCTCC TCCCCGGCCA GTCCCAGGTC TCGGTGAGCG TGGAGGACTG TGAGGACACT
    CGGGAGGCAA AGGGGCCCTG GGGTGCACGG GTGGGCACGA GCGGCGGCAG CACCCACGGC
    CGGCACACGG TGGGCAGCGA CCCAGCCGAG CACCCGCTCC TGCGGCGCAA GAGCCTCCCG
    TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC CGGCACACGG GGAATGCGGC AGCCGAGTGG
    ATCAGCCAAC AGCGGCTCAG CCTGTACCGG CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG
    GTCAAGAACC GCATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA TGTAGCAAAT
    TTAACAGCCA GTGATGTCAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA CGAGATGAAT
    GACCACCAGA ACACCCTCTC CTATGTGCTC ATCAACCCCC CGCCCGACAC GCGGCTGGAG
    CCCAATGACA TTGTATATCT CATCCGCTCC GACCCCTTGG CCCACGTGGC CAGCAGCTCC
    CAGAGCCGGA AGAGCAGCTG CAGCAACAAG CTGGCCTCCT GCAACCCTGA GACTCGGGAC
    GAGACACAGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855387.1
    CDS9..3743
    Translation

    Target ORF information:

    RefSeq Version XM_024999619.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG
    GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG
    GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC
    TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG
    GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC
    ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG
    CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG
    AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG
    CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC
    AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG
    GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT
    CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA
    AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC
    GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT
    CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG
    TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG
    TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC
    ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC
    CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC
    ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGGCC
    TCACCCCTCC TCCCCGGCCA GTCCCAGGTC TCGGTGAGCG TGGAGGACTG TGAGGACACT
    CGGGAGGCAA AGGGGCCCTG GGGTGCACGG GTGGGCACGA GCGGCGGCAG CACCCACGGC
    CGGCACACGG TGGGCAGCGA CCCAGCCGAG CACCCGCTCC TGCGGCGCAA GAGCCTCCCG
    TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC CGGCACACGG GGAATGCGGC AGCCGAGTGG
    ATCAGCCAAC AGCGGCTCAG CCTGTACCGG CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG
    GTCAAGAACC GCATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA TGTAGCAAAT
    TTAACAGCCA GTGATGTCAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA CGAGATGAAT
    GACCACCAGA ACACCCTCTC CTATGTGCTC ATCAACCCCC CGCCCGACAC GCGGCTGGAG
    CCCAATGACA TTGTATATCT CATCCGCTCC GACCCCTTGG CCCACGTGGC CAGCAGCTCC
    CAGAGCCGGA AGAGCAGCTG CAGCAACAAG CTGGCCTCCT GCAACCCTGA GACTCGGGAC
    GAGACACAGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220967
    Clone ID Related Accession (Same CDS sequence) XM_024999620.1
    Accession Version XM_024999620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG
    GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG
    GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC
    TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG
    GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC
    ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG
    CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG
    AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG
    CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC
    AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG
    GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT
    CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA
    AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC
    GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT
    CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG
    TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG
    TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC
    ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC
    CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC
    ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC
    CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT
    GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA
    GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG
    GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG
    TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG
    GGGCTGCCCA CCACTGGCTA CGAGGATGTA GCAAATTTAA CAGCCAGTGA TGTCATGAAT
    CGGGTAAACC TGGGATATTT GCAAGACGAG ATGAATGACC ACCAGAACAC CCTCTCCTAT
    GTGCTCATCA ACCCCCCGCC CGACACGCGG CTGGAGCCCA ATGACATTGT ATATCTCATC
    CGCTCCGACC CCTTGGCCCA CGTGGCCAGC AGCTCCCAGA GCCGGAAGAG CAGCTGCAGC
    AACAAGCTGG CCTCCTGCAA CCCTGAGACT CGGGACGAGA CACAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855388.1
    CDS9..3719
    Translation

    Target ORF information:

    RefSeq Version XM_024999620.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG
    GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG
    GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC
    TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG
    GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC
    ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG
    CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG
    AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG
    CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC
    AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG
    GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT
    CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA
    AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC
    GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT
    CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG
    TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG
    TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC
    ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC
    CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC
    ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC
    CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT
    GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA
    GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG
    GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG
    TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG
    GGGCTGCCCA CCACTGGCTA CGAGGATGTA GCAAATTTAA CAGCCAGTGA TGTCATGAAT
    CGGGTAAACC TGGGATATTT GCAAGACGAG ATGAATGACC ACCAGAACAC CCTCTCCTAT
    GTGCTCATCA ACCCCCCGCC CGACACGCGG CTGGAGCCCA ATGACATTGT ATATCTCATC
    CGCTCCGACC CCTTGGCCCA CGTGGCCAGC AGCTCCCAGA GCCGGAAGAG CAGCTGCAGC
    AACAAGCTGG CCTCCTGCAA CCCTGAGACT CGGGACGAGA CACAGCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220968
    Clone ID Related Accession (Same CDS sequence) XM_024999621.1
    Accession Version XM_024999621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GTGGCCATGG ACCTCCAGAA TACGGAGTGC
    CGACCCACAC AGAGTGGCGG GGGCAGTGGG GGCAGCAAGC TGGCGCTCCC CACGGAGAAC
    GGCTCAGGCA GCCGGCGGCC CAGCATCGCG CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC
    CTGCTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGACG AGATGACGCC GTCGGAGGAT
    GAGGGGCTGT CCGTGGTGGA GTATGTGAAG GGCTACCCTC CTAACTCACC CTACATCGGC
    AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG
    GACAAGGGCT GCAAGCACAA CAGCTACGAA GATGCCAAGG CCTATGGCTT CAAGAACAAG
    CTGATCATCG TCTCAGCGGA GACGGCGGGC AACGGGCTGT ACAACTTCAT CGTGCCGCTG
    CGCGCCTACT ACCGGCCTCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT GGACAACAAG
    CCCGACCACC ACTTCCTGGA GGCCATCTGC TGCTTTCCCA TGGTCTACTA CATGGAGGGC
    TCCGTGGACA ACCTGGACAG CCTGCTGCGG TGCGGTGTCA TCTACGCGGA CAATCTGGTG
    GTGGCAGACA AGGAGAGCAC CATGAGCGCT GAGGAGGACT ACATGGCCGA CGCAAAGACC
    ATCGTCAATG TGCAGACCAT GTTCCGGCTC TTCCCCAGTC TCAGCATCAC CACCGAGCTC
    ACCCACCCGT CCAACATGCG GTTCATGCAG TTTCGCGCCA AGGACAGCTA CTCTCTGGCT
    CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC
    CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC ATCAGCATGC TGGACACACT GCTGTATCAG
    TCCTTCGTGA AGGACTACAT GATCCCCATC ACCAGGCTGT TGCTGGGCTT GGACACCACG
    CCGGGCTCCG GCTACCTCTG TGCGATGAAG ATCTCGGAGG ACGACCTGTG GATCCGCACC
    TATGGCCGCC TCTTCCAGAA GCTGTGCTCC TCCAGCGCCG AGATCCCTAT CGGCATCTAC
    AGGACCCAGT GCCACGTCTT CTCCACCGAG CCCCCCGACC TCAGGGCCCA GTCCCAGGTC
    TCGGTGAGCG TGGAGGACTG TGAGGACACT CGGGAGGCAA AGGGGCCCTG GGGTGCACGG
    GTGGGCACGA GCGGCGGCAG CACCCACGGC CGGCACACGG TGGGCAGCGA CCCAGCCGAG
    CACCCGCTCC TGCGGCGCAA GAGCCTCCCG TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC
    CGGCACACGG GGAATGCGGC AGCCGAGTGG ATCAGCCAAC AGCGGCTCAG CCTGTACCGG
    CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG GTCAAGAACC GCATGAAGCA CCTGGGGCTG
    CCCACCACTG GCTACGAGGA TGTAGCAAAT TTAACAGCCA GTGATGTCAT GAATCGGGTA
    AACCTGGGAT ATTTGCAAGA CGAGATGAAT GACCACCAGA ACACCCTCTC CTATGTGCTC
    ATCAACCCCC CGCCCGACAC GCGGCTGGAG CCCAATGACA TTGTATATCT CATCCGCTCC
    GACCCCTTGG CCCACGTGGC CAGCAGCTCC CAGAGCCGGA AGAGCAGCTG CAGCAACAAG
    CTGGCCTCCT GCAACCCTGA GACTCGGGAC GAGACACAGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855389.1
    CDS9..3713
    Translation

    Target ORF information:

    RefSeq Version XM_024999621.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GTGGCCATGG ACCTCCAGAA TACGGAGTGC
    CGACCCACAC AGAGTGGCGG GGGCAGTGGG GGCAGCAAGC TGGCGCTCCC CACGGAGAAC
    GGCTCAGGCA GCCGGCGGCC CAGCATCGCG CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC
    CTGCTGCCCT GCGACCTGCT GAGTGACCAG TCAGAGGACG AGATGACGCC GTCGGAGGAT
    GAGGGGCTGT CCGTGGTGGA GTATGTGAAG GGCTACCCTC CTAACTCACC CTACATCGGC
    AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG
    GACAAGGGCT GCAAGCACAA CAGCTACGAA GATGCCAAGG CCTATGGCTT CAAGAACAAG
    CTGATCATCG TCTCAGCGGA GACGGCGGGC AACGGGCTGT ACAACTTCAT CGTGCCGCTG
    CGCGCCTACT ACCGGCCTCG CCGGGAGCTC AACCCCATTG TGCTGCTGCT GGACAACAAG
    CCCGACCACC ACTTCCTGGA GGCCATCTGC TGCTTTCCCA TGGTCTACTA CATGGAGGGC
    TCCGTGGACA ACCTGGACAG CCTGCTGCGG TGCGGTGTCA TCTACGCGGA CAATCTGGTG
    GTGGCAGACA AGGAGAGCAC CATGAGCGCT GAGGAGGACT ACATGGCCGA CGCAAAGACC
    ATCGTCAATG TGCAGACCAT GTTCCGGCTC TTCCCCAGTC TCAGCATCAC CACCGAGCTC
    ACCCACCCGT CCAACATGCG GTTCATGCAG TTTCGCGCCA AGGACAGCTA CTCTCTGGCT
    CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG AACGGCTCCA ACCTGGCCTT CATGTTCCGC
    CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC ATCAGCATGC TGGACACACT GCTGTATCAG
    TCCTTCGTGA AGGACTACAT GATCCCCATC ACCAGGCTGT TGCTGGGCTT GGACACCACG
    CCGGGCTCCG GCTACCTCTG TGCGATGAAG ATCTCGGAGG ACGACCTGTG GATCCGCACC
    TATGGCCGCC TCTTCCAGAA GCTGTGCTCC TCCAGCGCCG AGATCCCTAT CGGCATCTAC
    AGGACCCAGT GCCACGTCTT CTCCACCGAG CCCCCCGACC TCAGGGCCCA GTCCCAGGTC
    TCGGTGAGCG TGGAGGACTG TGAGGACACT CGGGAGGCAA AGGGGCCCTG GGGTGCACGG
    GTGGGCACGA GCGGCGGCAG CACCCACGGC CGGCACACGG TGGGCAGCGA CCCAGCCGAG
    CACCCGCTCC TGCGGCGCAA GAGCCTCCCG TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC
    CGGCACACGG GGAATGCGGC AGCCGAGTGG ATCAGCCAAC AGCGGCTCAG CCTGTACCGG
    CGCTCCGAGA GGCAGGAGCT CTCAGAGCTG GTCAAGAACC GCATGAAGCA CCTGGGGCTG
    CCCACCACTG GCTACGAGGA TGTAGCAAAT TTAACAGCCA GTGATGTCAT GAATCGGGTA
    AACCTGGGAT ATTTGCAAGA CGAGATGAAT GACCACCAGA ACACCCTCTC CTATGTGCTC
    ATCAACCCCC CGCCCGACAC GCGGCTGGAG CCCAATGACA TTGTATATCT CATCCGCTCC
    GACCCCTTGG CCCACGTGGC CAGCAGCTCC CAGAGCCGGA AGAGCAGCTG CAGCAACAAG
    CTGGCCTCCT GCAACCCTGA GACTCGGGAC GAGACACAGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220969
    Clone ID Related Accession (Same CDS sequence) XM_024999622.1
    Accession Version XM_024999622.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC
    ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC
    AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG
    CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG
    GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC
    CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA
    GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC
    AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG
    CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC
    ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT
    CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT
    GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG
    GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC
    AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC
    GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC
    TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC
    ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG
    CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG
    GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC
    GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC
    GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG
    GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC
    GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG
    CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG
    AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG
    CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC
    TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT
    TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855390.1
    CDS9..3701
    Translation

    Target ORF information:

    RefSeq Version XM_024999622.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999622.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC
    ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC
    AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG
    CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG
    GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC
    CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA
    GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC
    AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG
    CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC
    ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT
    CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT
    GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG
    GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC
    AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC
    GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC
    TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC
    ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG
    CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG
    GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC
    GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC
    GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG
    GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC
    GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG
    CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG
    AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG
    CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC
    TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT
    TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220970
    Clone ID Related Accession (Same CDS sequence) XM_024999623.1
    Accession Version XM_024999623.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC
    ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC
    AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG
    CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG
    GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC
    CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA
    GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC
    AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG
    CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC
    ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT
    CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT
    GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG
    GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC
    AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC
    GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC
    TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC
    ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG
    CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG
    GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC
    GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC
    GACCTCAGGG CCCAGTCCCA GGTCTCGGTG AGCGTGGAGG ACTGTGAGGA CACTCGGGAG
    GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC ACGAGCGGCG GCAGCACCCA CGGCCGGCAC
    ACGGTGGGCA GCGACCCAGC CGAGCACCCG CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG
    CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC ACGGGGAATG CGGCAGCCGA GTGGATCAGC
    CAACAGCGGC TCAGCCTGTA CCGGCGCTCC GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG
    AACCGCATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGATGTAGC AAATTTAACA
    GCCAGTGATG TCATGAATCG GGTAAACCTG GGATATTTGC AAGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855391.1
    CDS9..3677
    Translation

    Target ORF information:

    RefSeq Version XM_024999623.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999623.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC
    ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC
    AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG
    CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG
    GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC
    CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA
    GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC
    AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG
    CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC
    ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT
    CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT
    GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG
    GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC
    AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC
    GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC
    TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC
    ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG
    CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG
    GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC
    GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC
    GACCTCAGGG CCCAGTCCCA GGTCTCGGTG AGCGTGGAGG ACTGTGAGGA CACTCGGGAG
    GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC ACGAGCGGCG GCAGCACCCA CGGCCGGCAC
    ACGGTGGGCA GCGACCCAGC CGAGCACCCG CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG
    CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC ACGGGGAATG CGGCAGCCGA GTGGATCAGC
    CAACAGCGGC TCAGCCTGTA CCGGCGCTCC GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG
    AACCGCATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGATGTAGC AAATTTAACA
    GCCAGTGATG TCATGAATCG GGTAAACCTG GGATATTTGC AAGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220971
    Clone ID Related Accession (Same CDS sequence) XM_024999624.1
    Accession Version XM_024999624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG
    GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG
    GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC
    TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG
    GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC
    ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG
    CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG
    AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG
    CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC
    AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG
    GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT
    CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA
    AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC
    GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT
    CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG
    TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG
    TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC
    ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC
    CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC
    ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC
    CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT
    GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA
    GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG
    GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG
    TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG
    GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG
    CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC
    TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC
    AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855392.1
    CDS9..3656
    Translation

    Target ORF information:

    RefSeq Version XM_024999624.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
    CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC
    GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG
    GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG
    AGGCTTAAGC TGTTCTTCAT CAAAAACCAA AGATCCAGCC TGCGGATCCG GCTGTTCAAC
    TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA
    GCCCTGGGGA TCGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC
    TCCGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT
    CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC
    AAAGGCAACA TCTGGGAGCA GATCTTCCGC GTGTCCTTCA TCCTGGAGAT GATCAACACG
    CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT
    CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGTGCC
    ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCTTGCTG
    TGCCTGGTCT TCACAGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG
    TCCCTCCTGA CATCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC
    GTGACGCCCA AGATCTGGCC GTCCCAGCTG CTGGTGGTCA TCATGATCTG TGTGGCCCTG
    GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG
    GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC
    TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGATTG
    CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC
    CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT
    CAGGACCTCA TGCGGGCCAA GATGGACAAT GGGGAGGCCT GCTTTATTCT CAGCAGCCGG
    AACGAGGTGG ACCGCACCGC TGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG
    GACTTTGCCC CAAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT
    GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC
    AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC
    CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC
    GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC
    TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG
    CGCGAGGACA ATAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC
    GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG
    GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC
    CTGCCGGTGC ACAGCATCAT TGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAATACG
    GAGTGCCGAC CCACACAGAG TGGCGGGGGC AGTGGGGGCA GCAAGCTGGC GCTCCCCACG
    GAGAACGGCT CAGGCAGCCG GCGGCCCAGC ATCGCGCCGG TGCTGGAGGT GGCTGACAGC
    TCAGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG
    GAGGATGAGG GGCTGTCCGT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC
    ATCGGCAGCT CCCCAACCCT GTGCCATCTC CTGCCCGTGA AAGCCCCCTT CTGCTGCCTG
    CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA TGGCTTCAAG
    AACAAGCTGA TCATCGTCTC AGCGGAGACG GCGGGCAACG GGCTGTACAA CTTCATCGTG
    CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC
    AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG
    GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GTGTCATCTA CGCGGACAAT
    CTGGTGGTGG CAGACAAGGA GAGCACCATG AGCGCTGAGG AGGACTACAT GGCCGACGCA
    AAGACCATCG TCAATGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC
    GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT
    CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG
    TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG
    TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC
    ACCACGCCGG GCTCCGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC
    CGCACCTATG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC
    ATCTACAGGA CCCAGTGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC
    CAGGTCTCGG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT
    GCACGGGTGG GCACGAGCGG CGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA
    GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG
    GGCTCCCGGC ACACGGGGAA TGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG
    TACCGGCGCT CCGAGAGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG
    GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG
    CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC
    TCCGACCCCT TGGCCCACGT GGCCAGCAGC TCCCAGAGCC GGAAGAGCAG CTGCAGCAAC
    AAGCTGGCCT CCTGCAACCC TGAGACTCGG GACGAGACAC AGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220972
    Clone ID Related Accession (Same CDS sequence) XM_024999625.1
    Accession Version XM_024999625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
    CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC
    ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC
    AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG
    CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG
    GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC
    CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA
    GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC
    AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG
    CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC
    ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT
    CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT
    GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG
    GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC
    AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC
    GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC
    TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC
    ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG
    CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG
    GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC
    GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC
    GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG
    GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC
    GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG
    CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG
    AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG
    CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC
    TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT
    TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855393.1
    CDS31..3663
    Translation

    Target ORF information:

    RefSeq Version XM_024999625.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
    CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGG GACAGTGGCC
    ATGGACCTCC AGAATACGGA GTGCCGACCC ACACAGAGTG GCGGGGGCAG TGGGGGCAGC
    AAGCTGGCGC TCCCCACGGA GAACGGCTCA GGCAGCCGGC GGCCCAGCAT CGCGCCGGTG
    CTGGAGGTGG CTGACAGCTC AGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG
    GACGAGATGA CGCCGTCGGA GGATGAGGGG CTGTCCGTGG TGGAGTATGT GAAGGGCTAC
    CCTCCTAACT CACCCTACAT CGGCAGCTCC CCAACCCTGT GCCATCTCCT GCCCGTGAAA
    GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC
    AAGGCCTATG GCTTCAAGAA CAAGCTGATC ATCGTCTCAG CGGAGACGGC GGGCAACGGG
    CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC
    ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT
    CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGT
    GTCATCTACG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG CGCTGAGGAG
    GACTACATGG CCGACGCAAA GACCATCGTC AATGTGCAGA CCATGTTCCG GCTCTTCCCC
    AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC
    GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC
    TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC
    ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG
    CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCCGGCTACC TCTGTGCGAT GAAGATCTCG
    GAGGACGACC TGTGGATCCG CACCTATGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC
    GCCGAGATCC CTATCGGCAT CTACAGGACC CAGTGCCACG TCTTCTCCAC CGAGCCCCCC
    GACCTCAGGG CCCAGGCCTC ACCCCTCCTC CCCGGCCAGT CCCAGGTCTC GGTGAGCGTG
    GAGGACTGTG AGGACACTCG GGAGGCAAAG GGGCCCTGGG GTGCACGGGT GGGCACGAGC
    GGCGGCAGCA CCCACGGCCG GCACACGGTG GGCAGCGACC CAGCCGAGCA CCCGCTCCTG
    CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG CTGAGCCGCA AGGGCTCCCG GCACACGGGG
    AATGCGGCAG CCGAGTGGAT CAGCCAACAG CGGCTCAGCC TGTACCGGCG CTCCGAGAGG
    CAGGAGCTCT CAGAGCTGGT CAAGAACCGC ATGAAGCACC TGGGGCTGCC CACCACTGGC
    TACGAGGATG TAGCAAATTT AACAGCCAGT GATGTCATGA ATCGGGTAAA CCTGGGATAT
    TTGCAAGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220973
    Clone ID Related Accession (Same CDS sequence) XM_024999626.1
    Accession Version XM_024999626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
    CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGT GGCCATGGAC
    CTCCAGAATA CGGAGTGCCG ACCCACACAG AGTGGCGGGG GCAGTGGGGG CAGCAAGCTG
    GCGCTCCCCA CGGAGAACGG CTCAGGCAGC CGGCGGCCCA GCATCGCGCC GGTGCTGGAG
    GTGGCTGACA GCTCAGCCCT GCTGCCCTGC GACCTGCTGA GTGACCAGTC AGAGGACGAG
    ATGACGCCGT CGGAGGATGA GGGGCTGTCC GTGGTGGAGT ATGTGAAGGG CTACCCTCCT
    AACTCACCCT ACATCGGCAG CTCCCCAACC CTGTGCCATC TCCTGCCCGT GAAAGCCCCC
    TTCTGCTGCC TGCGGCTGGA CAAGGGCTGC AAGCACAACA GCTACGAAGA TGCCAAGGCC
    TATGGCTTCA AGAACAAGCT GATCATCGTC TCAGCGGAGA CGGCGGGCAA CGGGCTGTAC
    AACTTCATCG TGCCGCTGCG CGCCTACTAC CGGCCTCGCC GGGAGCTCAA CCCCATTGTG
    CTGCTGCTGG ACAACAAGCC CGACCACCAC TTCCTGGAGG CCATCTGCTG CTTTCCCATG
    GTCTACTACA TGGAGGGCTC CGTGGACAAC CTGGACAGCC TGCTGCGGTG CGGTGTCATC
    TACGCGGACA ATCTGGTGGT GGCAGACAAG GAGAGCACCA TGAGCGCTGA GGAGGACTAC
    ATGGCCGACG CAAAGACCAT CGTCAATGTG CAGACCATGT TCCGGCTCTT CCCCAGTCTC
    AGCATCACCA CCGAGCTCAC CCACCCGTCC AACATGCGGT TCATGCAGTT TCGCGCCAAG
    GACAGCTACT CTCTGGCTCT CTCTAAACTG GAGAAGAGGG AGCGTGAGAA CGGCTCCAAC
    CTGGCCTTCA TGTTCCGCCT GCCCTTCGCC GCCGGCCGCG TCTTCAGCAT CAGCATGCTG
    GACACACTGC TGTATCAGTC CTTCGTGAAG GACTACATGA TCCCCATCAC CAGGCTGTTG
    CTGGGCTTGG ACACCACGCC GGGCTCCGGC TACCTCTGTG CGATGAAGAT CTCGGAGGAC
    GACCTGTGGA TCCGCACCTA TGGCCGCCTC TTCCAGAAGC TGTGCTCCTC CAGCGCCGAG
    ATCCCTATCG GCATCTACAG GACCCAGTGC CACGTCTTCT CCACCGAGCC CCCCGACCTC
    AGGGCCCAGT CCCAGGTCTC GGTGAGCGTG GAGGACTGTG AGGACACTCG GGAGGCAAAG
    GGGCCCTGGG GTGCACGGGT GGGCACGAGC GGCGGCAGCA CCCACGGCCG GCACACGGTG
    GGCAGCGACC CAGCCGAGCA CCCGCTCCTG CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG
    CTGAGCCGCA AGGGCTCCCG GCACACGGGG AATGCGGCAG CCGAGTGGAT CAGCCAACAG
    CGGCTCAGCC TGTACCGGCG CTCCGAGAGG CAGGAGCTCT CAGAGCTGGT CAAGAACCGC
    ATGAAGCACC TGGGGCTGCC CACCACTGGC TACGAGGATG TAGCAAATTT AACAGCCAGT
    GATGTCATGA ATCGGGTAAA CCTGGGATAT TTGCAAGACG AGATGAATGA CCACCAGAAC
    ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT
    GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG
    AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855394.1
    CDS30..3632
    Translation

    Target ORF information:

    RefSeq Version XM_024999626.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
    CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC
    GAGAACACCT TCAAGGAGAG GCTTAAGCTG TTCTTCATCA AAAACCAAAG ATCCAGCCTG
    CGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC
    GTCCTGCTGG ACGACCCAGC CCTGGGGATC GGATGCTGGG GCTGCCCGAA GCAGAATTAC
    ACCTTCAATG AGTCGTCCTC CGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG
    GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT
    CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGCGT GTCCTTCATC
    CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC
    CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC
    AACGACTTCC ACCGTGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC
    CTCTTCTGCA CCTTGCTGTG CCTGGTCTTC ACAGGGACCT GCGGCATCCA GCACCTGGAG
    CGCGCGGGCG ACAACCTGTC CCTCCTGACA TCCTTCTACT TCTGCATCGT CACCTTCTCC
    ACCGTGGGCT ATGGGGACGT GACGCCCAAG ATCTGGCCGT CCCAGCTGCT GGTGGTCATC
    ATGATCTGTG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG
    ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT
    GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC
    TACGCGCATC CACGATTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC
    ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG
    GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAATGG GGAGGCCTGC
    TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCTG CGGACCACCA GACCATACTG
    CGTGCCTGGG CCGTGAAGGA CTTTGCCCCA AACTGCCCCC TCTACGTCCA GATCCTCAAG
    CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG
    TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG
    GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC
    GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC
    GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG
    TGCCTCATCG GCCTGAAGCG CGAGGACAAT AAGAGCATCC TGCTGAACCC GGGCCCACGG
    CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG
    GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG
    TACGAGGGCT CATCCCGCCT GCCGGTGCAC AGCATCATTG CCTCCATGGT GGCCATGGAC
    CTCCAGAATA CGGAGTGCCG ACCCACACAG AGTGGCGGGG GCAGTGGGGG CAGCAAGCTG
    GCGCTCCCCA CGGAGAACGG CTCAGGCAGC CGGCGGCCCA GCATCGCGCC GGTGCTGGAG
    GTGGCTGACA GCTCAGCCCT GCTGCCCTGC GACCTGCTGA GTGACCAGTC AGAGGACGAG
    ATGACGCCGT CGGAGGATGA GGGGCTGTCC GTGGTGGAGT ATGTGAAGGG CTACCCTCCT
    AACTCACCCT ACATCGGCAG CTCCCCAACC CTGTGCCATC TCCTGCCCGT GAAAGCCCCC
    TTCTGCTGCC TGCGGCTGGA CAAGGGCTGC AAGCACAACA GCTACGAAGA TGCCAAGGCC
    TATGGCTTCA AGAACAAGCT GATCATCGTC TCAGCGGAGA CGGCGGGCAA CGGGCTGTAC
    AACTTCATCG TGCCGCTGCG CGCCTACTAC CGGCCTCGCC GGGAGCTCAA CCCCATTGTG
    CTGCTGCTGG ACAACAAGCC CGACCACCAC TTCCTGGAGG CCATCTGCTG CTTTCCCATG
    GTCTACTACA TGGAGGGCTC CGTGGACAAC CTGGACAGCC TGCTGCGGTG CGGTGTCATC
    TACGCGGACA ATCTGGTGGT GGCAGACAAG GAGAGCACCA TGAGCGCTGA GGAGGACTAC
    ATGGCCGACG CAAAGACCAT CGTCAATGTG CAGACCATGT TCCGGCTCTT CCCCAGTCTC
    AGCATCACCA CCGAGCTCAC CCACCCGTCC AACATGCGGT TCATGCAGTT TCGCGCCAAG
    GACAGCTACT CTCTGGCTCT CTCTAAACTG GAGAAGAGGG AGCGTGAGAA CGGCTCCAAC
    CTGGCCTTCA TGTTCCGCCT GCCCTTCGCC GCCGGCCGCG TCTTCAGCAT CAGCATGCTG
    GACACACTGC TGTATCAGTC CTTCGTGAAG GACTACATGA TCCCCATCAC CAGGCTGTTG
    CTGGGCTTGG ACACCACGCC GGGCTCCGGC TACCTCTGTG CGATGAAGAT CTCGGAGGAC
    GACCTGTGGA TCCGCACCTA TGGCCGCCTC TTCCAGAAGC TGTGCTCCTC CAGCGCCGAG
    ATCCCTATCG GCATCTACAG GACCCAGTGC CACGTCTTCT CCACCGAGCC CCCCGACCTC
    AGGGCCCAGT CCCAGGTCTC GGTGAGCGTG GAGGACTGTG AGGACACTCG GGAGGCAAAG
    GGGCCCTGGG GTGCACGGGT GGGCACGAGC GGCGGCAGCA CCCACGGCCG GCACACGGTG
    GGCAGCGACC CAGCCGAGCA CCCGCTCCTG CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG
    CTGAGCCGCA AGGGCTCCCG GCACACGGGG AATGCGGCAG CCGAGTGGAT CAGCCAACAG
    CGGCTCAGCC TGTACCGGCG CTCCGAGAGG CAGGAGCTCT CAGAGCTGGT CAAGAACCGC
    ATGAAGCACC TGGGGCTGCC CACCACTGGC TACGAGGATG TAGCAAATTT AACAGCCAGT
    GATGTCATGA ATCGGGTAAA CCTGGGATAT TTGCAAGACG AGATGAATGA CCACCAGAAC
    ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT
    GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG
    AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220974
    Clone ID Related Accession (Same CDS sequence) XM_015473691.2
    Accession Version XM_015473691.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    ACTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329177.2
    CDS908..4486
    Translation

    Target ORF information:

    RefSeq Version XM_015473691.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473691.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    ACTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220975
    Clone ID Related Accession (Same CDS sequence) XM_024999627.1
    Accession Version XM_024999627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
    AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTGTTCTTCA TCAAAAACCA AAGATCCAGC
    CTGCGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC
    CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATCGGATGCT GGGGCTGCCC GAAGCAGAAT
    TACACCTTCA ATGAGTCGTC CTCCGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG
    AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG
    CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG CGTGTCCTTC
    ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG
    AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG
    ATCAACGACT TCCACCGTGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC
    ATCCTCTTCT GCACCTTGCT GTGCCTGGTC TTCACAGGGA CCTGCGGCAT CCAGCACCTG
    GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACATCCTTCT ACTTCTGCAT CGTCACCTTC
    TCCACCGTGG GCTATGGGGA CGTGACGCCC AAGATCTGGC CGTCCCAGCT GCTGGTGGTC
    ATCATGATCT GTGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG
    TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG
    CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG
    TTCTACGCGC ATCCACGATT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG
    GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC
    CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA TGGGGAGGCC
    TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CTGCGGACCA CCAGACCATA
    CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCAAACTGCC CCCTCTACGT CCAGATCCTC
    AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC
    AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG
    CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG
    TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC
    CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC
    GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AATAAGAGCA TCCTGCTGAA CCCGGGCCCA
    CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC
    TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG
    CTGTACGAGG GCTCATCCCG CCTGCCGGTG CACAGCATCA TTGCCTCCAT GGGGACAGTG
    GCCATGGACC TCCAGAATAC GGAGTGCCGA CCCACACAGA GTGGCGGGGG CAGTGGGGGC
    AGCAAGCTGG CGCTCCCCAC GGAGAACGGC TCAGGCAGCC GGCGGCCCAG CATCGCGCCG
    GTGCTGGAGG TGGCTGACAG CTCAGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA
    GAGGACGAGA TGACGCCGTC GGAGGATGAG GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC
    TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCAACCC TGTGCCATCT CCTGCCCGTG
    AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT
    GCCAAGGCCT ATGGCTTCAA GAACAAGCTG ATCATCGTCT CAGCGGAGAC GGCGGGCAAC
    GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC
    CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC
    TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC
    GGTGTCATCT ACGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGCGCTGAG
    GAGGACTACA TGGCCGACGC AAAGACCATC GTCAATGTGC AGACCATGTT CCGGCTCTTC
    CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT
    CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC
    GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC
    AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC
    AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCCGGCT ACCTCTGTGC GATGAAGATC
    TCGGAGGACG ACCTGTGGAT CCGCACCTAT GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC
    AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAGTGCC ACGTCTTCTC CACCGAGCCC
    CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT
    GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA
    TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC
    CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG
    GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC
    TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855395.1
    CDS208..3783
    Translation

    Target ORF information:

    RefSeq Version XM_024999627.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
    AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTGTTCTTCA TCAAAAACCA AAGATCCAGC
    CTGCGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC
    CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATCGGATGCT GGGGCTGCCC GAAGCAGAAT
    TACACCTTCA ATGAGTCGTC CTCCGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG
    AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG
    CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG CGTGTCCTTC
    ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG
    AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG
    ATCAACGACT TCCACCGTGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC
    ATCCTCTTCT GCACCTTGCT GTGCCTGGTC TTCACAGGGA CCTGCGGCAT CCAGCACCTG
    GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACATCCTTCT ACTTCTGCAT CGTCACCTTC
    TCCACCGTGG GCTATGGGGA CGTGACGCCC AAGATCTGGC CGTCCCAGCT GCTGGTGGTC
    ATCATGATCT GTGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG
    TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG
    CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG
    TTCTACGCGC ATCCACGATT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG
    GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC
    CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA TGGGGAGGCC
    TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CTGCGGACCA CCAGACCATA
    CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCAAACTGCC CCCTCTACGT CCAGATCCTC
    AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC
    AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG
    CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG
    TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC
    CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC
    GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AATAAGAGCA TCCTGCTGAA CCCGGGCCCA
    CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC
    TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG
    CTGTACGAGG GCTCATCCCG CCTGCCGGTG CACAGCATCA TTGCCTCCAT GGGGACAGTG
    GCCATGGACC TCCAGAATAC GGAGTGCCGA CCCACACAGA GTGGCGGGGG CAGTGGGGGC
    AGCAAGCTGG CGCTCCCCAC GGAGAACGGC TCAGGCAGCC GGCGGCCCAG CATCGCGCCG
    GTGCTGGAGG TGGCTGACAG CTCAGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA
    GAGGACGAGA TGACGCCGTC GGAGGATGAG GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC
    TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCAACCC TGTGCCATCT CCTGCCCGTG
    AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT
    GCCAAGGCCT ATGGCTTCAA GAACAAGCTG ATCATCGTCT CAGCGGAGAC GGCGGGCAAC
    GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC
    CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC
    TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC
    GGTGTCATCT ACGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGCGCTGAG
    GAGGACTACA TGGCCGACGC AAAGACCATC GTCAATGTGC AGACCATGTT CCGGCTCTTC
    CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT
    CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC
    GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC
    AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC
    AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCCGGCT ACCTCTGTGC GATGAAGATC
    TCGGAGGACG ACCTGTGGAT CCGCACCTAT GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC
    AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAGTGCC ACGTCTTCTC CACCGAGCCC
    CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT
    GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA
    TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC
    CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG
    GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC
    TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220976
    Clone ID Related Accession (Same CDS sequence) XM_024999628.1
    Accession Version XM_024999628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC
    GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG
    CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC
    AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG
    AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG
    CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG
    AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT
    GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC
    CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA
    TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC
    AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855396.1
    CDS1472..5011
    Translation

    Target ORF information:

    RefSeq Version XM_024999628.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC
    GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG
    CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC
    AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG
    AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG
    CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG
    AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT
    GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC
    CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA
    TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC
    AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220977
    Clone ID Related Accession (Same CDS sequence) XM_024999629.1
    Accession Version XM_024999629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGACATC GAGGCACCTG GCTTTACATC CCCAGGGAGA CCAGCTGGGG CTGCCCGAAG 
    CAGAATTACA CCTTCAATGA GTCGTCCTCC GAGATCAACT GGGCCCCCAT CCTGTGGGTG
    GAGAGGAAGG TGGCTCTGTG GGCCATTCAG GTCATCGTGG CCATCATCAG CTTCCTGGAG
    ACCATGCTTC TCATTTACCT CAGCTACAAA GGCAACATCT GGGAGCAGAT CTTCCGCGTG
    TCCTTCATCC TGGAGATGAT CAACACGCTG CCCTTCATCA TCACGATATT CTGGCCGCCG
    CTGCGGAACC TGTTCATCCC CGTGTTTCTG AACTGCTGGC TGGCCAAGCA CGCCCTGGAG
    AACATGATCA ACGACTTCCA CCGTGCCATC CTGCGCTCGC AGTCGGCCAT GTTCAATCAG
    GTCCTCATCC TCTTCTGCAC CTTGCTGTGC CTGGTCTTCA CAGGGACCTG CGGCATCCAG
    CACCTGGAGC GCGCGGGCGA CAACCTGTCC CTCCTGACAT CCTTCTACTT CTGCATCGTC
    ACCTTCTCCA CCGTGGGCTA TGGGGACGTG ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG
    GTGGTCATCA TGATCTGTGT GGCCCTGGTG GTGCTCCCGC TGCAGTTTGA GGAACTCGTC
    TACCTGTGGA TGGAGCGGCA GAAGTCGGGG GGCAACTACA GCCGCCACCG GGCCCAGACG
    GAGAAGCATG TGATCCTGTG CGTGAGCTCC CTCAAGATCG ACCTGCTCAT GGACTTTCTG
    AACGAGTTCT ACGCGCATCC ACGATTGCAG GACTACTACG TCGTCATCCT GTGCCCCACG
    GAGATGGACA TCCAGGTTCG CAGGGTCCTG CAGATCCCAC TGTGGTCCCA GCGGGTCATC
    TACCTCCAGG GCTCCGCGCT CAAGGATCAG GACCTCATGC GGGCCAAGAT GGACAATGGG
    GAGGCCTGCT TTATTCTCAG CAGCCGGAAC GAGGTGGACC GCACCGCTGC GGACCACCAG
    ACCATACTGC GTGCCTGGGC CGTGAAGGAC TTTGCCCCAA ACTGCCCCCT CTACGTCCAG
    ATCCTCAAGC CTGAGAACAA GTTTCATGTC AAGTTTGCAG AGCACGTGGT GTGCGAGGAG
    GAGTGCAAGT ATGCCATGCT GGCCCTCAAC TGCATCTGCC CAGCCACGTC CACCCTCATC
    ACCCTGCTGG TGCACACGTC CCGCGGCCAG GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG
    CGCATGTACG GACGCTGCTC GGGCAACGAG GTCTACCACG TGCGGATGGG GGACAGCAAG
    TTCTTCCGCG AGTACGAGGG CAAGAGCTTC ACCTACGCCG CCTTCCACGC GCACAAGAAG
    TACGGCGTGT GCCTCATCGG CCTGAAGCGC GAGGACAATA AGAGCATCCT GCTGAACCCG
    GGCCCACGGC ACATCCTGGC GGCCTCCGAC ACCTGCTTCT ACATCAACAT CACCAAGGAG
    GAGAACTCGG CCTTCATCTT CAAGCAGGAG GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG
    CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG CCGGTGCACA GCATCATTGC CTCCATGGGG
    ACAGTGGCCA TGGACCTCCA GAATACGGAG TGCCGACCCA CACAGAGTGG CGGGGGCAGT
    GGGGGCAGCA AGCTGGCGCT CCCCACGGAG AACGGCTCAG GCAGCCGGCG GCCCAGCATC
    GCGCCGGTGC TGGAGGTGGC TGACAGCTCA GCCCTGCTGC CCTGCGACCT GCTGAGTGAC
    CAGTCAGAGG ACGAGATGAC GCCGTCGGAG GATGAGGGGC TGTCCGTGGT GGAGTATGTG
    AAGGGCTACC CTCCTAACTC ACCCTACATC GGCAGCTCCC CAACCCTGTG CCATCTCCTG
    CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG CTGGACAAGG GCTGCAAGCA CAACAGCTAC
    GAAGATGCCA AGGCCTATGG CTTCAAGAAC AAGCTGATCA TCGTCTCAGC GGAGACGGCG
    GGCAACGGGC TGTACAACTT CATCGTGCCG CTGCGCGCCT ACTACCGGCC TCGCCGGGAG
    CTCAACCCCA TTGTGCTGCT GCTGGACAAC AAGCCCGACC ACCACTTCCT GGAGGCCATC
    TGCTGCTTTC CCATGGTCTA CTACATGGAG GGCTCCGTGG ACAACCTGGA CAGCCTGCTG
    CGGTGCGGTG TCATCTACGC GGACAATCTG GTGGTGGCAG ACAAGGAGAG CACCATGAGC
    GCTGAGGAGG ACTACATGGC CGACGCAAAG ACCATCGTCA ATGTGCAGAC CATGTTCCGG
    CTCTTCCCCA GTCTCAGCAT CACCACCGAG CTCACCCACC CGTCCAACAT GCGGTTCATG
    CAGTTTCGCG CCAAGGACAG CTACTCTCTG GCTCTCTCTA AACTGGAGAA GAGGGAGCGT
    GAGAACGGCT CCAACCTGGC CTTCATGTTC CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC
    AGCATCAGCA TGCTGGACAC ACTGCTGTAT CAGTCCTTCG TGAAGGACTA CATGATCCCC
    ATCACCAGGC TGTTGCTGGG CTTGGACACC ACGCCGGGCT CCGGCTACCT CTGTGCGATG
    AAGATCTCGG AGGACGACCT GTGGATCCGC ACCTATGGCC GCCTCTTCCA GAAGCTGTGC
    TCCTCCAGCG CCGAGATCCC TATCGGCATC TACAGGACCC AGTGCCACGT CTTCTCCACC
    GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG
    GTGAGCGTGG AGGACTGTGA GGACACTCGG GAGGCAAAGG GGCCCTGGGG TGCACGGGTG
    GGCACGAGCG GCGGCAGCAC CCACGGCCGG CACACGGTGG GCAGCGACCC AGCCGAGCAC
    CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG
    CACACGGGGA ATGCGGCAGC CGAGTGGATC AGCCAACAGC GGCTCAGCCT GTACCGGCGC
    TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC AAGAACCGCA TGAAGCACCT GGGGCTGCCC
    ACCACTGGCT ACGAGGATGT AGCAAATTTA ACAGCCAGTG ATGTCATGAA TCGGGTAAAC
    CTGGGATATT TGCAAGACGA GATGAATGAC CACCAGAACA CCCTCTCCTA TGTGCTCATC
    AACCCCCCGC CCGACACGCG GCTGGAGCCC AATGACATTG TATATCTCAT CCGCTCCGAC
    CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG AGCCGGAAGA GCAGCTGCAG CAACAAGCTG
    GCCTCCTGCA ACCCTGAGAC TCGGGACGAG ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855397.1
    CDS139..3540
    Translation

    Target ORF information:

    RefSeq Version XM_024999629.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGACATC GAGGCACCTG GCTTTACATC CCCAGGGAGA CCAGCTGGGG CTGCCCGAAG 
    CAGAATTACA CCTTCAATGA GTCGTCCTCC GAGATCAACT GGGCCCCCAT CCTGTGGGTG
    GAGAGGAAGG TGGCTCTGTG GGCCATTCAG GTCATCGTGG CCATCATCAG CTTCCTGGAG
    ACCATGCTTC TCATTTACCT CAGCTACAAA GGCAACATCT GGGAGCAGAT CTTCCGCGTG
    TCCTTCATCC TGGAGATGAT CAACACGCTG CCCTTCATCA TCACGATATT CTGGCCGCCG
    CTGCGGAACC TGTTCATCCC CGTGTTTCTG AACTGCTGGC TGGCCAAGCA CGCCCTGGAG
    AACATGATCA ACGACTTCCA CCGTGCCATC CTGCGCTCGC AGTCGGCCAT GTTCAATCAG
    GTCCTCATCC TCTTCTGCAC CTTGCTGTGC CTGGTCTTCA CAGGGACCTG CGGCATCCAG
    CACCTGGAGC GCGCGGGCGA CAACCTGTCC CTCCTGACAT CCTTCTACTT CTGCATCGTC
    ACCTTCTCCA CCGTGGGCTA TGGGGACGTG ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG
    GTGGTCATCA TGATCTGTGT GGCCCTGGTG GTGCTCCCGC TGCAGTTTGA GGAACTCGTC
    TACCTGTGGA TGGAGCGGCA GAAGTCGGGG GGCAACTACA GCCGCCACCG GGCCCAGACG
    GAGAAGCATG TGATCCTGTG CGTGAGCTCC CTCAAGATCG ACCTGCTCAT GGACTTTCTG
    AACGAGTTCT ACGCGCATCC ACGATTGCAG GACTACTACG TCGTCATCCT GTGCCCCACG
    GAGATGGACA TCCAGGTTCG CAGGGTCCTG CAGATCCCAC TGTGGTCCCA GCGGGTCATC
    TACCTCCAGG GCTCCGCGCT CAAGGATCAG GACCTCATGC GGGCCAAGAT GGACAATGGG
    GAGGCCTGCT TTATTCTCAG CAGCCGGAAC GAGGTGGACC GCACCGCTGC GGACCACCAG
    ACCATACTGC GTGCCTGGGC CGTGAAGGAC TTTGCCCCAA ACTGCCCCCT CTACGTCCAG
    ATCCTCAAGC CTGAGAACAA GTTTCATGTC AAGTTTGCAG AGCACGTGGT GTGCGAGGAG
    GAGTGCAAGT ATGCCATGCT GGCCCTCAAC TGCATCTGCC CAGCCACGTC CACCCTCATC
    ACCCTGCTGG TGCACACGTC CCGCGGCCAG GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG
    CGCATGTACG GACGCTGCTC GGGCAACGAG GTCTACCACG TGCGGATGGG GGACAGCAAG
    TTCTTCCGCG AGTACGAGGG CAAGAGCTTC ACCTACGCCG CCTTCCACGC GCACAAGAAG
    TACGGCGTGT GCCTCATCGG CCTGAAGCGC GAGGACAATA AGAGCATCCT GCTGAACCCG
    GGCCCACGGC ACATCCTGGC GGCCTCCGAC ACCTGCTTCT ACATCAACAT CACCAAGGAG
    GAGAACTCGG CCTTCATCTT CAAGCAGGAG GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG
    CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG CCGGTGCACA GCATCATTGC CTCCATGGGG
    ACAGTGGCCA TGGACCTCCA GAATACGGAG TGCCGACCCA CACAGAGTGG CGGGGGCAGT
    GGGGGCAGCA AGCTGGCGCT CCCCACGGAG AACGGCTCAG GCAGCCGGCG GCCCAGCATC
    GCGCCGGTGC TGGAGGTGGC TGACAGCTCA GCCCTGCTGC CCTGCGACCT GCTGAGTGAC
    CAGTCAGAGG ACGAGATGAC GCCGTCGGAG GATGAGGGGC TGTCCGTGGT GGAGTATGTG
    AAGGGCTACC CTCCTAACTC ACCCTACATC GGCAGCTCCC CAACCCTGTG CCATCTCCTG
    CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG CTGGACAAGG GCTGCAAGCA CAACAGCTAC
    GAAGATGCCA AGGCCTATGG CTTCAAGAAC AAGCTGATCA TCGTCTCAGC GGAGACGGCG
    GGCAACGGGC TGTACAACTT CATCGTGCCG CTGCGCGCCT ACTACCGGCC TCGCCGGGAG
    CTCAACCCCA TTGTGCTGCT GCTGGACAAC AAGCCCGACC ACCACTTCCT GGAGGCCATC
    TGCTGCTTTC CCATGGTCTA CTACATGGAG GGCTCCGTGG ACAACCTGGA CAGCCTGCTG
    CGGTGCGGTG TCATCTACGC GGACAATCTG GTGGTGGCAG ACAAGGAGAG CACCATGAGC
    GCTGAGGAGG ACTACATGGC CGACGCAAAG ACCATCGTCA ATGTGCAGAC CATGTTCCGG
    CTCTTCCCCA GTCTCAGCAT CACCACCGAG CTCACCCACC CGTCCAACAT GCGGTTCATG
    CAGTTTCGCG CCAAGGACAG CTACTCTCTG GCTCTCTCTA AACTGGAGAA GAGGGAGCGT
    GAGAACGGCT CCAACCTGGC CTTCATGTTC CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC
    AGCATCAGCA TGCTGGACAC ACTGCTGTAT CAGTCCTTCG TGAAGGACTA CATGATCCCC
    ATCACCAGGC TGTTGCTGGG CTTGGACACC ACGCCGGGCT CCGGCTACCT CTGTGCGATG
    AAGATCTCGG AGGACGACCT GTGGATCCGC ACCTATGGCC GCCTCTTCCA GAAGCTGTGC
    TCCTCCAGCG CCGAGATCCC TATCGGCATC TACAGGACCC AGTGCCACGT CTTCTCCACC
    GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG
    GTGAGCGTGG AGGACTGTGA GGACACTCGG GAGGCAAAGG GGCCCTGGGG TGCACGGGTG
    GGCACGAGCG GCGGCAGCAC CCACGGCCGG CACACGGTGG GCAGCGACCC AGCCGAGCAC
    CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG
    CACACGGGGA ATGCGGCAGC CGAGTGGATC AGCCAACAGC GGCTCAGCCT GTACCGGCGC
    TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC AAGAACCGCA TGAAGCACCT GGGGCTGCCC
    ACCACTGGCT ACGAGGATGT AGCAAATTTA ACAGCCAGTG ATGTCATGAA TCGGGTAAAC
    CTGGGATATT TGCAAGACGA GATGAATGAC CACCAGAACA CCCTCTCCTA TGTGCTCATC
    AACCCCCCGC CCGACACGCG GCTGGAGCCC AATGACATTG TATATCTCAT CCGCTCCGAC
    CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG AGCCGGAAGA GCAGCTGCAG CAACAAGCTG
    GCCTCCTGCA ACCCTGAGAC TCGGGACGAG ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220978
    Clone ID Related Accession (Same CDS sequence) XM_024999630.1
    Accession Version XM_024999630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GACTCAGGTT CCTGGTGGCA TCATCGTTTC AATATCCGCG
    GCACCTGTGA CAGTGACGTC GACCAGGGCC CAGCCTGCCC GTTCAGTTCT GCTCCCTCCA
    GACACGTGGT TCTCAGAAGG GCTCGTCCAC GTGGGTTCCC ATGTCTCGCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855398.1
    CDS659..3892
    Translation

    Target ORF information:

    RefSeq Version XM_024999630.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GACTCAGGTT CCTGGTGGCA TCATCGTTTC AATATCCGCG
    GCACCTGTGA CAGTGACGTC GACCAGGGCC CAGCCTGCCC GTTCAGTTCT GCTCCCTCCA
    GACACGTGGT TCTCAGAAGG GCTCGTCCAC GTGGGTTCCC ATGTCTCGCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220979
    Clone ID Related Accession (Same CDS sequence) XM_024999631.1
    Accession Version XM_024999631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCGCTTC ACGACGGGGT GCGCAGGGGT CCAGGAGATG TCTACAGCGG GGTGCAAGGC 
    GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCAGCCC
    GGCGCCGAGG ACGGCGGCCG CGGGGACGGC GCGCGCCTGG ACACCGCCGG CCTCAAGATG
    AGCGACCTGG ACTCGGAGGT GCTGCCCTTG CCTCCGCGGT ACCGCTTCCG GGACCTGCTG
    CTGGGCGACC AGTCCTTCCA GAACGACGAC AGGGTCCAGG TGGAGTTCTA CGTCAACGAG
    AACACCTTCA AGGAGAGGCT TAAGCTGTTC TTCATCAAAA ACCAAAGATC CAGCCTGCGG
    ATCCGGCTGT TCAACTTCTC CCTGAAGCTG CTGACCTGCC TGCTGTACAT TGTCCGCGTC
    CTGCTGGACG ACCCAGCCCT GGGGATCGGA TGCTGGGGCT GCCCGAAGCA GAATTACACC
    TTCAATGAGT CGTCCTCCGA GATCAACTGG GCCCCCATCC TGTGGGTGGA GAGGAAGGTG
    GCTCTGTGGG CCATTCAGGT CATCGTGGCC ATCATCAGCT TCCTGGAGAC CATGCTTCTC
    ATTTACCTCA GCTACAAAGG CAACATCTGG GAGCAGATCT TCCGCGTGTC CTTCATCCTG
    GAGATGATCA ACACGCTGCC CTTCATCATC ACGATATTCT GGCCGCCGCT GCGGAACCTG
    TTCATCCCCG TGTTTCTGAA CTGCTGGCTG GCCAAGCACG CCCTGGAGAA CATGATCAAC
    GACTTCCACC GTGCCATCCT GCGCTCGCAG TCGGCCATGT TCAATCAGGT CCTCATCCTC
    TTCTGCACCT TGCTGTGCCT GGTCTTCACA GGGACCTGCG GCATCCAGCA CCTGGAGCGC
    GCGGGCGACA ACCTGTCCCT CCTGACATCC TTCTACTTCT GCATCGTCAC CTTCTCCACC
    GTGGGCTATG GGGACGTGAC GCCCAAGATC TGGCCGTCCC AGCTGCTGGT GGTCATCATG
    ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG CAGTTTGAGG AACTCGTCTA CCTGTGGATG
    GAGCGGCAGA AGTCGGGGGG CAACTACAGC CGCCACCGGG CCCAGACGGA GAAGCATGTG
    ATCCTGTGCG TGAGCTCCCT CAAGATCGAC CTGCTCATGG ACTTTCTGAA CGAGTTCTAC
    GCGCATCCAC GATTGCAGGA CTACTACGTC GTCATCCTGT GCCCCACGGA GATGGACATC
    CAGGTTCGCA GGGTCCTGCA GATCCCACTG TGGTCCCAGC GGGTCATCTA CCTCCAGGGC
    TCCGCGCTCA AGGATCAGGA CCTCATGCGG GCCAAGATGG ACAATGGGGA GGCCTGCTTT
    ATTCTCAGCA GCCGGAACGA GGTGGACCGC ACCGCTGCGG ACCACCAGAC CATACTGCGT
    GCCTGGGCCG TGAAGGACTT TGCCCCAAAC TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT
    GAGAACAAGT TTCATGTCAA GTTTGCAGAG CACGTGGTGT GCGAGGAGGA GTGCAAGTAT
    GCCATGCTGG CCCTCAACTG CATCTGCCCA GCCACGTCCA CCCTCATCAC CCTGCTGGTG
    CACACGTCCC GCGGCCAGGA AGGCCAGGAC TCGCCAGAGC AGTGGCAGCG CATGTACGGA
    CGCTGCTCGG GCAACGAGGT CTACCACGTG CGGATGGGGG ACAGCAAGTT CTTCCGCGAG
    TACGAGGGCA AGAGCTTCAC CTACGCCGCC TTCCACGCGC ACAAGAAGTA CGGCGTGTGC
    CTCATCGGCC TGAAGCGCGA GGACAATAAG AGCATCCTGC TGAACCCGGG CCCACGGCAC
    ATCCTGGCGG CCTCCGACAC CTGCTTCTAC ATCAACATCA CCAAGGAGGA GAACTCGGCC
    TTCATCTTCA AGCAGGAGGA AAAGCAGAAG AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC
    GAGGGCTCAT CCCGCCTGCC GGTGCACAGC ATCATTGCCT CCATGGGGAC AGTGGCCATG
    GACCTCCAGA ATACGGAGTG CCGACCCACA CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG
    CTGGCGCTCC CCACGGAGAA CGGCTCAGGC AGCCGGCGGC CCAGCATCGC GCCGGTGCTG
    GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAC
    GAGATGACGC CGTCGGAGGA TGAGGGGCTG TCCGTGGTGG AGTATGTGAA GGGCTACCCT
    CCTAACTCAC CCTACATCGG CAGCTCCCCA ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC
    CCCTTCTGCT GCCTGCGGCT GGACAAGGGC TGCAAGCACA ACAGCTACGA AGATGCCAAG
    GCCTATGGCT TCAAGAACAA GCTGATCATC GTCTCAGCGG AGACGGCGGG CAACGGGCTG
    TACAACTTCA TCGTGCCGCT GCGCGCCTAC TACCGGCCTC GCCGGGAGCT CAACCCCATT
    GTGCTGCTGC TGGACAACAA GCCCGACCAC CACTTCCTGG AGGCCATCTG CTGCTTTCCC
    ATGGTCTACT ACATGGAGGG CTCCGTGGAC AACCTGGACA GCCTGCTGCG GTGCGGTGTC
    ATCTACGCGG ACAATCTGGT GGTGGCAGAC AAGGAGAGCA CCATGAGCGC TGAGGAGGAC
    TACATGGCCG ACGCAAAGAC CATCGTCAAT GTGCAGACCA TGTTCCGGCT CTTCCCCAGT
    CTCAGCATCA CCACCGAGCT CACCCACCCG TCCAACATGC GGTTCATGCA GTTTCGCGCC
    AAGGACAGCT ACTCTCTGGC TCTCTCTAAA CTGGAGAAGA GGGAGCGTGA GAACGGCTCC
    AACCTGGCCT TCATGTTCCG CCTGCCCTTC GCCGCCGGCC GCGTCTTCAG CATCAGCATG
    CTGGACACAC TGCTGTATCA GTCCTTCGTG AAGGACTACA TGATCCCCAT CACCAGGCTG
    TTGCTGGGCT TGGACACCAC GCCGGGCTCC GGCTACCTCT GTGCGATGAA GATCTCGGAG
    GACGACCTGT GGATCCGCAC CTATGGCCGC CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC
    GAGATCCCTA TCGGCATCTA CAGGACCCAG TGCCACGTCT TCTCCACCGA GCCCCCCGAC
    CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGACG AGATGAATGA CCACCAGAAC
    ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT
    GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG
    AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855399.1
    CDS25..3747
    Translation

    Target ORF information:

    RefSeq Version XM_024999631.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCGCTTC ACGACGGGGT GCGCAGGGGT CCAGGAGATG TCTACAGCGG GGTGCAAGGC 
    GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCAGCCC
    GGCGCCGAGG ACGGCGGCCG CGGGGACGGC GCGCGCCTGG ACACCGCCGG CCTCAAGATG
    AGCGACCTGG ACTCGGAGGT GCTGCCCTTG CCTCCGCGGT ACCGCTTCCG GGACCTGCTG
    CTGGGCGACC AGTCCTTCCA GAACGACGAC AGGGTCCAGG TGGAGTTCTA CGTCAACGAG
    AACACCTTCA AGGAGAGGCT TAAGCTGTTC TTCATCAAAA ACCAAAGATC CAGCCTGCGG
    ATCCGGCTGT TCAACTTCTC CCTGAAGCTG CTGACCTGCC TGCTGTACAT TGTCCGCGTC
    CTGCTGGACG ACCCAGCCCT GGGGATCGGA TGCTGGGGCT GCCCGAAGCA GAATTACACC
    TTCAATGAGT CGTCCTCCGA GATCAACTGG GCCCCCATCC TGTGGGTGGA GAGGAAGGTG
    GCTCTGTGGG CCATTCAGGT CATCGTGGCC ATCATCAGCT TCCTGGAGAC CATGCTTCTC
    ATTTACCTCA GCTACAAAGG CAACATCTGG GAGCAGATCT TCCGCGTGTC CTTCATCCTG
    GAGATGATCA ACACGCTGCC CTTCATCATC ACGATATTCT GGCCGCCGCT GCGGAACCTG
    TTCATCCCCG TGTTTCTGAA CTGCTGGCTG GCCAAGCACG CCCTGGAGAA CATGATCAAC
    GACTTCCACC GTGCCATCCT GCGCTCGCAG TCGGCCATGT TCAATCAGGT CCTCATCCTC
    TTCTGCACCT TGCTGTGCCT GGTCTTCACA GGGACCTGCG GCATCCAGCA CCTGGAGCGC
    GCGGGCGACA ACCTGTCCCT CCTGACATCC TTCTACTTCT GCATCGTCAC CTTCTCCACC
    GTGGGCTATG GGGACGTGAC GCCCAAGATC TGGCCGTCCC AGCTGCTGGT GGTCATCATG
    ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG CAGTTTGAGG AACTCGTCTA CCTGTGGATG
    GAGCGGCAGA AGTCGGGGGG CAACTACAGC CGCCACCGGG CCCAGACGGA GAAGCATGTG
    ATCCTGTGCG TGAGCTCCCT CAAGATCGAC CTGCTCATGG ACTTTCTGAA CGAGTTCTAC
    GCGCATCCAC GATTGCAGGA CTACTACGTC GTCATCCTGT GCCCCACGGA GATGGACATC
    CAGGTTCGCA GGGTCCTGCA GATCCCACTG TGGTCCCAGC GGGTCATCTA CCTCCAGGGC
    TCCGCGCTCA AGGATCAGGA CCTCATGCGG GCCAAGATGG ACAATGGGGA GGCCTGCTTT
    ATTCTCAGCA GCCGGAACGA GGTGGACCGC ACCGCTGCGG ACCACCAGAC CATACTGCGT
    GCCTGGGCCG TGAAGGACTT TGCCCCAAAC TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT
    GAGAACAAGT TTCATGTCAA GTTTGCAGAG CACGTGGTGT GCGAGGAGGA GTGCAAGTAT
    GCCATGCTGG CCCTCAACTG CATCTGCCCA GCCACGTCCA CCCTCATCAC CCTGCTGGTG
    CACACGTCCC GCGGCCAGGA AGGCCAGGAC TCGCCAGAGC AGTGGCAGCG CATGTACGGA
    CGCTGCTCGG GCAACGAGGT CTACCACGTG CGGATGGGGG ACAGCAAGTT CTTCCGCGAG
    TACGAGGGCA AGAGCTTCAC CTACGCCGCC TTCCACGCGC ACAAGAAGTA CGGCGTGTGC
    CTCATCGGCC TGAAGCGCGA GGACAATAAG AGCATCCTGC TGAACCCGGG CCCACGGCAC
    ATCCTGGCGG CCTCCGACAC CTGCTTCTAC ATCAACATCA CCAAGGAGGA GAACTCGGCC
    TTCATCTTCA AGCAGGAGGA AAAGCAGAAG AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC
    GAGGGCTCAT CCCGCCTGCC GGTGCACAGC ATCATTGCCT CCATGGGGAC AGTGGCCATG
    GACCTCCAGA ATACGGAGTG CCGACCCACA CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG
    CTGGCGCTCC CCACGGAGAA CGGCTCAGGC AGCCGGCGGC CCAGCATCGC GCCGGTGCTG
    GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAC
    GAGATGACGC CGTCGGAGGA TGAGGGGCTG TCCGTGGTGG AGTATGTGAA GGGCTACCCT
    CCTAACTCAC CCTACATCGG CAGCTCCCCA ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC
    CCCTTCTGCT GCCTGCGGCT GGACAAGGGC TGCAAGCACA ACAGCTACGA AGATGCCAAG
    GCCTATGGCT TCAAGAACAA GCTGATCATC GTCTCAGCGG AGACGGCGGG CAACGGGCTG
    TACAACTTCA TCGTGCCGCT GCGCGCCTAC TACCGGCCTC GCCGGGAGCT CAACCCCATT
    GTGCTGCTGC TGGACAACAA GCCCGACCAC CACTTCCTGG AGGCCATCTG CTGCTTTCCC
    ATGGTCTACT ACATGGAGGG CTCCGTGGAC AACCTGGACA GCCTGCTGCG GTGCGGTGTC
    ATCTACGCGG ACAATCTGGT GGTGGCAGAC AAGGAGAGCA CCATGAGCGC TGAGGAGGAC
    TACATGGCCG ACGCAAAGAC CATCGTCAAT GTGCAGACCA TGTTCCGGCT CTTCCCCAGT
    CTCAGCATCA CCACCGAGCT CACCCACCCG TCCAACATGC GGTTCATGCA GTTTCGCGCC
    AAGGACAGCT ACTCTCTGGC TCTCTCTAAA CTGGAGAAGA GGGAGCGTGA GAACGGCTCC
    AACCTGGCCT TCATGTTCCG CCTGCCCTTC GCCGCCGGCC GCGTCTTCAG CATCAGCATG
    CTGGACACAC TGCTGTATCA GTCCTTCGTG AAGGACTACA TGATCCCCAT CACCAGGCTG
    TTGCTGGGCT TGGACACCAC GCCGGGCTCC GGCTACCTCT GTGCGATGAA GATCTCGGAG
    GACGACCTGT GGATCCGCAC CTATGGCCGC CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC
    GAGATCCCTA TCGGCATCTA CAGGACCCAG TGCCACGTCT TCTCCACCGA GCCCCCCGAC
    CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGACG AGATGAATGA CCACCAGAAC
    ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT
    GTATATCTCA TCCGCTCCGA CCCCTTGGCC CACGTGGCCA GCAGCTCCCA GAGCCGGAAG
    AGCAGCTGCA GCAACAAGCT GGCCTCCTGC AACCCTGAGA CTCGGGACGA GACACAGCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92023
    Clone ID Related Accession (Same CDS sequence) XM_015465523.1 , XM_015473687.1
    Accession Version XM_015465523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC
    CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC
    AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG
    AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG
    ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321009.1
    CDS104..4135
    Translation

    Target ORF information:

    RefSeq Version XM_015465523.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC
    CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC
    AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG
    AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG
    ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92023
    Clone ID Related Accession (Same CDS sequence) XM_015465523.1 , XM_015473687.1
    Accession Version XM_015473687.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC
    CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC
    AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG
    AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG
    ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329173.1
    CDS104..4135
    Translation

    Target ORF information:

    RefSeq Version XM_015473687.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473687.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGACGA GATGAATGAC
    CACCAGAACA CCCTCTCCTA TGTGCTCATC AACCCCCCGC CCGACACGCG GCTGGAGCCC
    AATGACATTG TATATCTCAT CCGCTCCGAC CCCTTGGCCC ACGTGGCCAG CAGCTCCCAG
    AGCCGGAAGA GCAGCTGCAG CAACAAGCTG GCCTCCTGCA ACCCTGAGAC TCGGGACGAG
    ACACAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92024
    Clone ID Related Accession (Same CDS sequence) XM_015473688.1 , XM_015465524.1
    Accession Version XM_015473688.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4026bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA
    CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC
    TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG
    CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG
    GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC
    TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC
    AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG
    ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC
    GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC
    GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC
    GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG
    GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC
    GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC
    CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC
    TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG
    CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC
    TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG
    GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT
    GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG
    ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC
    CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC
    AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG
    AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC
    ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG
    AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329174.1
    CDS104..4129
    Translation

    Target ORF information:

    RefSeq Version XM_015473688.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473688.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA
    CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC
    TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG
    CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG
    GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC
    TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC
    AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG
    ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC
    GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC
    GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC
    GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG
    GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC
    GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC
    CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC
    TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG
    CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC
    TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG
    GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT
    GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG
    ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC
    CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC
    AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG
    AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC
    ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG
    AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92024
    Clone ID Related Accession (Same CDS sequence) XM_015473688.1 , XM_015465524.1
    Accession Version XM_015465524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4026bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA
    CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC
    TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG
    CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG
    GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC
    TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC
    AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG
    ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC
    GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC
    GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC
    GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG
    GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC
    GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC
    CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC
    TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG
    CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC
    TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG
    GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT
    GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG
    ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC
    CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC
    AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG
    AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC
    ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG
    AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321010.1
    CDS104..4129
    Translation

    Target ORF information:

    RefSeq Version XM_015465524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGTG GCCATGGACC TCCAGAATAC GGAGTGCCGA
    CCCACACAGA GTGGCGGGGG CAGTGGGGGC AGCAAGCTGG CGCTCCCCAC GGAGAACGGC
    TCAGGCAGCC GGCGGCCCAG CATCGCGCCG GTGCTGGAGG TGGCTGACAG CTCAGCCCTG
    CTGCCCTGCG ACCTGCTGAG TGACCAGTCA GAGGACGAGA TGACGCCGTC GGAGGATGAG
    GGGCTGTCCG TGGTGGAGTA TGTGAAGGGC TACCCTCCTA ACTCACCCTA CATCGGCAGC
    TCCCCAACCC TGTGCCATCT CCTGCCCGTG AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC
    AAGGGCTGCA AGCACAACAG CTACGAAGAT GCCAAGGCCT ATGGCTTCAA GAACAAGCTG
    ATCATCGTCT CAGCGGAGAC GGCGGGCAAC GGGCTGTACA ACTTCATCGT GCCGCTGCGC
    GCCTACTACC GGCCTCGCCG GGAGCTCAAC CCCATTGTGC TGCTGCTGGA CAACAAGCCC
    GACCACCACT TCCTGGAGGC CATCTGCTGC TTTCCCATGG TCTACTACAT GGAGGGCTCC
    GTGGACAACC TGGACAGCCT GCTGCGGTGC GGTGTCATCT ACGCGGACAA TCTGGTGGTG
    GCAGACAAGG AGAGCACCAT GAGCGCTGAG GAGGACTACA TGGCCGACGC AAAGACCATC
    GTCAATGTGC AGACCATGTT CCGGCTCTTC CCCAGTCTCA GCATCACCAC CGAGCTCACC
    CACCCGTCCA ACATGCGGTT CATGCAGTTT CGCGCCAAGG ACAGCTACTC TCTGGCTCTC
    TCTAAACTGG AGAAGAGGGA GCGTGAGAAC GGCTCCAACC TGGCCTTCAT GTTCCGCCTG
    CCCTTCGCCG CCGGCCGCGT CTTCAGCATC AGCATGCTGG ACACACTGCT GTATCAGTCC
    TTCGTGAAGG ACTACATGAT CCCCATCACC AGGCTGTTGC TGGGCTTGGA CACCACGCCG
    GGCTCCGGCT ACCTCTGTGC GATGAAGATC TCGGAGGACG ACCTGTGGAT CCGCACCTAT
    GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC AGCGCCGAGA TCCCTATCGG CATCTACAGG
    ACCCAGTGCC ACGTCTTCTC CACCGAGCCC CCCGACCTCA GGGCCCAGGC CTCACCCCTC
    CTCCCCGGCC AGTCCCAGGT CTCGGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA
    AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGCGGCA GCACCCACGG CCGGCACACG
    GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG
    AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAATGCGG CAGCCGAGTG GATCAGCCAA
    CAGCGGCTCA GCCTGTACCG GCGCTCCGAG AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC
    CGCATGAAGC ACCTGGGGCT GCCCACCAGT GGCTACGAGG ATGTAGCAAA TTTAACAGCC
    AGTGATGTCA TGAATCGGGT AAACCTGGGA TATTTGCAAG ACGAGATGAA TGACCACCAG
    AACACCCTCT CCTATGTGCT CATCAACCCC CCGCCCGACA CGCGGCTGGA GCCCAATGAC
    ATTGTATATC TCATCCGCTC CGACCCCTTG GCCCACGTGG CCAGCAGCTC CCAGAGCCGG
    AAGAGCAGCT GCAGCAACAA GCTGGCCTCC TGCAACCCTG AGACTCGGGA CGAGACACAG
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92025
    Clone ID Related Accession (Same CDS sequence) XM_010819338.2 , XM_010810537.2
    Accession Version XM_010819338.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA
    TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC
    CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG
    GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC
    TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817640.2
    CDS82..3837
    Translation

    Target ORF information:

    RefSeq Version XM_010819338.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819338.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA
    TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC
    CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG
    GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC
    TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92025
    Clone ID Related Accession (Same CDS sequence) XM_010819338.2 , XM_010810537.2
    Accession Version XM_010810537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA
    TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC
    CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG
    GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC
    TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808839.2
    CDS82..3837
    Translation

    Target ORF information:

    RefSeq Version XM_010810537.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA
    TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC
    CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG
    GCCCACGTGG CCAGCAGCTC CCAGAGCCGG AAGAGCAGCT GCAGCAACAA GCTGGCCTCC
    TGCAACCCTG AGACTCGGGA CGAGACACAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92026
    Clone ID Related Accession (Same CDS sequence) XM_015465525.1 , XM_015473689.1
    Accession Version XM_015465525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321011.1
    CDS104..4072
    Translation

    Target ORF information:

    RefSeq Version XM_015465525.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92026
    Clone ID Related Accession (Same CDS sequence) XM_015465525.1 , XM_015473689.1
    Accession Version XM_015473689.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329175.1
    CDS104..4072
    Translation

    Target ORF information:

    RefSeq Version XM_015473689.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473689.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGACGAGAT GAATGACCAC
    CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT
    GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCACG TGGCCAGCAG CTCCCAGAGC
    CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC TCCTGCAACC CTGAGACTCG GGACGAGACA
    CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92027
    Clone ID Related Accession (Same CDS sequence) XM_010810539.2 , XM_010819346.2
    Accession Version XM_010810539.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808841.2
    CDS82..3774
    Translation

    Target ORF information:

    RefSeq Version XM_010810539.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810539.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92027
    Clone ID Related Accession (Same CDS sequence) XM_010810539.2 , XM_010819346.2
    Accession Version XM_010819346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817648.2
    CDS82..3774
    Translation

    Target ORF information:

    RefSeq Version XM_010819346.2
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTACCCAC ACTGCCGTGG GCGCTGTGCT GCTTCTGGGG CATCTGCGGG CCAGGCCCCA 
    CCAGGGGGTG TGCGCATGGG CGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC
    TGTGTCCGGG AGCCCAGGCT CTCCCATCTG TCCATCCGTC CGCTGTCTGT GTTGATGTGT
    CCGCTCCATT CCGCCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT
    CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCGGTGAGC
    GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG
    AGCGGCGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC
    CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG
    GGGAATGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCCGAG
    AGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCAGT
    GGCTACGACG AGATGAATGA CCACCAGAAC ACCCTCTCCT ATGTGCTCAT CAACCCCCCG
    CCCGACACGC GGCTGGAGCC CAATGACATT GTATATCTCA TCCGCTCCGA CCCCTTGGCC
    CACGTGGCCA GCAGCTCCCA GAGCCGGAAG AGCAGCTGCA GCAACAAGCT GGCCTCCTGC
    AACCCTGAGA CTCGGGACGA GACACAGCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92028
    Clone ID Related Accession (Same CDS sequence) XM_015465526.1 , XM_015473690.1
    Accession Version XM_015465526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA
    GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT
    ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321012.1
    CDS104..4042
    Translation

    Target ORF information:

    RefSeq Version XM_015465526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA
    GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT
    ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92028
    Clone ID Related Accession (Same CDS sequence) XM_015465526.1 , XM_015473690.1
    Accession Version XM_015473690.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA
    GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT
    ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329176.1
    CDS104..4042
    Translation

    Target ORF information:

    RefSeq Version XM_015473690.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCCAGCT GGTGGCCCCT GTGTGTGGGG TGCCTCTCGC TGCCATCAGC TTCCTGGGTG 
    TCGAGGGGGG CTCACCTGCT CCTTCCTGGA CCCTGGGGTC ACACGAGGCC TGGGGCAGTC
    TCAGTGCAGA GCAGAGCTGT GGGCGCTCCT GAACCTGCTG TCCAGCCACT CGCTTGGCTG
    CAGGCGGGGG CCCGTCCTGC GTGCCATCTG CTGGAATCGT CACCCTGTCC CACCGGGGTG
    GGCACGGGGT GCATGTACCC ACACTGCCGT GGGCGCTGTG CTGCTTCTGG GGCATCTGCG
    GGCCAGGCCC CACCAGGGGG TGTGCGCATG GGCGTGCCCT GCTCCCCTCG GCCCCTGCCC
    GAGTCCTCCC TCTGTGTCCG GGAGCCCAGG CTCTCCCATC TGTCCATCCG TCCGCTGTCT
    GTGTTGATGT GTCCGCTCCA TTCCGCCCTC AGGCTTCATG TGGAAGAGTT CAGCCTGGAC
    TCCTCTGTGT CTCAGGTCCA GGTGGAGTTC TACGTCAACG AGAACACCTT CAAGGAGAGG
    CTTAAGCTGT TCTTCATCAA AAACCAAAGA TCCAGCCTGC GGATCCGGCT GTTCAACTTC
    TCCCTGAAGC TGCTGACCTG CCTGCTGTAC ATTGTCCGCG TCCTGCTGGA CGACCCAGCC
    CTGGGGATCG GATGCTGGGG CTGCCCGAAG CAGAATTACA CCTTCAATGA GTCGTCCTCC
    GAGATCAACT GGGCCCCCAT CCTGTGGGTG GAGAGGAAGG TGGCTCTGTG GGCCATTCAG
    GTCATCGTGG CCATCATCAG CTTCCTGGAG ACCATGCTTC TCATTTACCT CAGCTACAAA
    GGCAACATCT GGGAGCAGAT CTTCCGCGTG TCCTTCATCC TGGAGATGAT CAACACGCTG
    CCCTTCATCA TCACGATATT CTGGCCGCCG CTGCGGAACC TGTTCATCCC CGTGTTTCTG
    AACTGCTGGC TGGCCAAGCA CGCCCTGGAG AACATGATCA ACGACTTCCA CCGTGCCATC
    CTGCGCTCGC AGTCGGCCAT GTTCAATCAG GTCCTCATCC TCTTCTGCAC CTTGCTGTGC
    CTGGTCTTCA CAGGGACCTG CGGCATCCAG CACCTGGAGC GCGCGGGCGA CAACCTGTCC
    CTCCTGACAT CCTTCTACTT CTGCATCGTC ACCTTCTCCA CCGTGGGCTA TGGGGACGTG
    ACGCCCAAGA TCTGGCCGTC CCAGCTGCTG GTGGTCATCA TGATCTGTGT GGCCCTGGTG
    GTGCTCCCGC TGCAGTTTGA GGAACTCGTC TACCTGTGGA TGGAGCGGCA GAAGTCGGGG
    GGCAACTACA GCCGCCACCG GGCCCAGACG GAGAAGCATG TGATCCTGTG CGTGAGCTCC
    CTCAAGATCG ACCTGCTCAT GGACTTTCTG AACGAGTTCT ACGCGCATCC ACGATTGCAG
    GACTACTACG TCGTCATCCT GTGCCCCACG GAGATGGACA TCCAGGTTCG CAGGGTCCTG
    CAGATCCCAC TGTGGTCCCA GCGGGTCATC TACCTCCAGG GCTCCGCGCT CAAGGATCAG
    GACCTCATGC GGGCCAAGAT GGACAATGGG GAGGCCTGCT TTATTCTCAG CAGCCGGAAC
    GAGGTGGACC GCACCGCTGC GGACCACCAG ACCATACTGC GTGCCTGGGC CGTGAAGGAC
    TTTGCCCCAA ACTGCCCCCT CTACGTCCAG ATCCTCAAGC CTGAGAACAA GTTTCATGTC
    AAGTTTGCAG AGCACGTGGT GTGCGAGGAG GAGTGCAAGT ATGCCATGCT GGCCCTCAAC
    TGCATCTGCC CAGCCACGTC CACCCTCATC ACCCTGCTGG TGCACACGTC CCGCGGCCAG
    GAAGGCCAGG ACTCGCCAGA GCAGTGGCAG CGCATGTACG GACGCTGCTC GGGCAACGAG
    GTCTACCACG TGCGGATGGG GGACAGCAAG TTCTTCCGCG AGTACGAGGG CAAGAGCTTC
    ACCTACGCCG CCTTCCACGC GCACAAGAAG TACGGCGTGT GCCTCATCGG CCTGAAGCGC
    GAGGACAATA AGAGCATCCT GCTGAACCCG GGCCCACGGC ACATCCTGGC GGCCTCCGAC
    ACCTGCTTCT ACATCAACAT CACCAAGGAG GAGAACTCGG CCTTCATCTT CAAGCAGGAG
    GAAAAGCAGA AGAGGAAGGG CTTCGCGGGG CAGGGGCTGT ACGAGGGCTC ATCCCGCCTG
    CCGGTGCACA GCATCATTGC CTCCATGGGG ACAGTGGCCA TGGACCTCCA GAATACGGAG
    TGCCGACCCA CACAGAGTGG CGGGGGCAGT GGGGGCAGCA AGCTGGCGCT CCCCACGGAG
    AACGGCTCAG GCAGCCGGCG GCCCAGCATC GCGCCGGTGC TGGAGGTGGC TGACAGCTCA
    GCCCTGCTGC CCTGCGACCT GCTGAGTGAC CAGTCAGAGG ACGAGATGAC GCCGTCGGAG
    GATGAGGGGC TGTCCGTGGT GGAGTATGTG AAGGGCTACC CTCCTAACTC ACCCTACATC
    GGCAGCTCCC CAACCCTGTG CCATCTCCTG CCCGTGAAAG CCCCCTTCTG CTGCCTGCGG
    CTGGACAAGG GCTGCAAGCA CAACAGCTAC GAAGATGCCA AGGCCTATGG CTTCAAGAAC
    AAGCTGATCA TCGTCTCAGC GGAGACGGCG GGCAACGGGC TGTACAACTT CATCGTGCCG
    CTGCGCGCCT ACTACCGGCC TCGCCGGGAG CTCAACCCCA TTGTGCTGCT GCTGGACAAC
    AAGCCCGACC ACCACTTCCT GGAGGCCATC TGCTGCTTTC CCATGGTCTA CTACATGGAG
    GGCTCCGTGG ACAACCTGGA CAGCCTGCTG CGGTGCGGTG TCATCTACGC GGACAATCTG
    GTGGTGGCAG ACAAGGAGAG CACCATGAGC GCTGAGGAGG ACTACATGGC CGACGCAAAG
    ACCATCGTCA ATGTGCAGAC CATGTTCCGG CTCTTCCCCA GTCTCAGCAT CACCACCGAG
    CTCACCCACC CGTCCAACAT GCGGTTCATG CAGTTTCGCG CCAAGGACAG CTACTCTCTG
    GCTCTCTCTA AACTGGAGAA GAGGGAGCGT GAGAACGGCT CCAACCTGGC CTTCATGTTC
    CGCCTGCCCT TCGCCGCCGG CCGCGTCTTC AGCATCAGCA TGCTGGACAC ACTGCTGTAT
    CAGTCCTTCG TGAAGGACTA CATGATCCCC ATCACCAGGC TGTTGCTGGG CTTGGACACC
    ACGCCGGGCT CCGGCTACCT CTGTGCGATG AAGATCTCGG AGGACGACCT GTGGATCCGC
    ACCTATGGCC GCCTCTTCCA GAAGCTGTGC TCCTCCAGCG CCGAGATCCC TATCGGCATC
    TACAGGACCC AGTGCCACGT CTTCTCCACC GAGCCCCCCG ACCTCAGGGC CCAGGCCTCA
    CCCCTCCTCC CCGGCCAGTC CCAGGTCTCG GTGAGCGTGG AGGACTGTGA GGACACTCGG
    GAGGCAAAGG GGCCCTGGGG TGCACGGGTG GGCACGAGCG GCGGCAGCAC CCACGGCCGG
    CACACGGTGG GCAGCGACCC AGCCGAGCAC CCGCTCCTGC GGCGCAAGAG CCTCCCGTGG
    GCGCGGAGGC TGAGCCGCAA GGGCTCCCGG CACACGGGGA ATGCGGCAGC CGAGTGGATC
    AGCCAACAGC GGCTCAGCCT GTACCGGCGC TCCGAGAGGC AGGAGCTCTC AGAGCTGGTC
    AAGAACCGCA TGAAGCACCT GGGGCTGCCC ACCAGTGGCT ACGAGGATGT AGCAAATTTA
    ACAGCCAGTG ATGTCATGAA TCGGGTAAAC CTGGGATATT TGCAAGGCAG CCACTGGCCA
    GCCCCTGGGA GCCCACCCAG CTCTCAAGAA GCACCGGCAT GGAGCACCCC AGGCCAGGTT
    ACCCGCGCCC CCGGCATGGT GGAGCACAGT GAGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92029
    Clone ID Related Accession (Same CDS sequence) XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
    Accession Version XM_015465528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321014.1
    CDS147..3725
    Translation

    Target ORF information:

    RefSeq Version XM_015465528.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92029
    Clone ID Related Accession (Same CDS sequence) XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
    Accession Version XM_015473691.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329177.1
    CDS119..3697
    Translation

    Target ORF information:

    RefSeq Version XM_015473691.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92029
    Clone ID Related Accession (Same CDS sequence) XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
    Accession Version XM_015465527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321013.1
    CDS119..3697
    Translation

    Target ORF information:

    RefSeq Version XM_015465527.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92029
    Clone ID Related Accession (Same CDS sequence) XM_015465528.1 , XM_015473691.1 , XM_015465527.1 , XM_015473692.1
    Accession Version XM_015473692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329178.1
    CDS147..3725
    Translation

    Target ORF information:

    RefSeq Version XM_015473692.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCTGGGG CAGGCGGTTG CTGTCACTGC CTGTTCCCCA GGGTCCAGGT GGAGTTCTAC 
    GTCAACGAGA ACACCTTCAA GGAGAGGCTT AAGCTGTTCT TCATCAAAAA CCAAAGATCC
    AGCCTGCGGA TCCGGCTGTT CAACTTCTCC CTGAAGCTGC TGACCTGCCT GCTGTACATT
    GTCCGCGTCC TGCTGGACGA CCCAGCCCTG GGGATCGGAT GCTGGGGCTG CCCGAAGCAG
    AATTACACCT TCAATGAGTC GTCCTCCGAG ATCAACTGGG CCCCCATCCT GTGGGTGGAG
    AGGAAGGTGG CTCTGTGGGC CATTCAGGTC ATCGTGGCCA TCATCAGCTT CCTGGAGACC
    ATGCTTCTCA TTTACCTCAG CTACAAAGGC AACATCTGGG AGCAGATCTT CCGCGTGTCC
    TTCATCCTGG AGATGATCAA CACGCTGCCC TTCATCATCA CGATATTCTG GCCGCCGCTG
    CGGAACCTGT TCATCCCCGT GTTTCTGAAC TGCTGGCTGG CCAAGCACGC CCTGGAGAAC
    ATGATCAACG ACTTCCACCG TGCCATCCTG CGCTCGCAGT CGGCCATGTT CAATCAGGTC
    CTCATCCTCT TCTGCACCTT GCTGTGCCTG GTCTTCACAG GGACCTGCGG CATCCAGCAC
    CTGGAGCGCG CGGGCGACAA CCTGTCCCTC CTGACATCCT TCTACTTCTG CATCGTCACC
    TTCTCCACCG TGGGCTATGG GGACGTGACG CCCAAGATCT GGCCGTCCCA GCTGCTGGTG
    GTCATCATGA TCTGTGTGGC CCTGGTGGTG CTCCCGCTGC AGTTTGAGGA ACTCGTCTAC
    CTGTGGATGG AGCGGCAGAA GTCGGGGGGC AACTACAGCC GCCACCGGGC CCAGACGGAG
    AAGCATGTGA TCCTGTGCGT GAGCTCCCTC AAGATCGACC TGCTCATGGA CTTTCTGAAC
    GAGTTCTACG CGCATCCACG ATTGCAGGAC TACTACGTCG TCATCCTGTG CCCCACGGAG
    ATGGACATCC AGGTTCGCAG GGTCCTGCAG ATCCCACTGT GGTCCCAGCG GGTCATCTAC
    CTCCAGGGCT CCGCGCTCAA GGATCAGGAC CTCATGCGGG CCAAGATGGA CAATGGGGAG
    GCCTGCTTTA TTCTCAGCAG CCGGAACGAG GTGGACCGCA CCGCTGCGGA CCACCAGACC
    ATACTGCGTG CCTGGGCCGT GAAGGACTTT GCCCCAAACT GCCCCCTCTA CGTCCAGATC
    CTCAAGCCTG AGAACAAGTT TCATGTCAAG TTTGCAGAGC ACGTGGTGTG CGAGGAGGAG
    TGCAAGTATG CCATGCTGGC CCTCAACTGC ATCTGCCCAG CCACGTCCAC CCTCATCACC
    CTGCTGGTGC ACACGTCCCG CGGCCAGGAA GGCCAGGACT CGCCAGAGCA GTGGCAGCGC
    ATGTACGGAC GCTGCTCGGG CAACGAGGTC TACCACGTGC GGATGGGGGA CAGCAAGTTC
    TTCCGCGAGT ACGAGGGCAA GAGCTTCACC TACGCCGCCT TCCACGCGCA CAAGAAGTAC
    GGCGTGTGCC TCATCGGCCT GAAGCGCGAG GACAATAAGA GCATCCTGCT GAACCCGGGC
    CCACGGCACA TCCTGGCGGC CTCCGACACC TGCTTCTACA TCAACATCAC CAAGGAGGAG
    AACTCGGCCT TCATCTTCAA GCAGGAGGAA AAGCAGAAGA GGAAGGGCTT CGCGGGGCAG
    GGGCTGTACG AGGGCTCATC CCGCCTGCCG GTGCACAGCA TCATTGCCTC CATGGGGACA
    GTGGCCATGG ACCTCCAGAA TACGGAGTGC CGACCCACAC AGAGTGGCGG GGGCAGTGGG
    GGCAGCAAGC TGGCGCTCCC CACGGAGAAC GGCTCAGGCA GCCGGCGGCC CAGCATCGCG
    CCGGTGCTGG AGGTGGCTGA CAGCTCAGCC CTGCTGCCCT GCGACCTGCT GAGTGACCAG
    TCAGAGGACG AGATGACGCC GTCGGAGGAT GAGGGGCTGT CCGTGGTGGA GTATGTGAAG
    GGCTACCCTC CTAACTCACC CTACATCGGC AGCTCCCCAA CCCTGTGCCA TCTCCTGCCC
    GTGAAAGCCC CCTTCTGCTG CCTGCGGCTG GACAAGGGCT GCAAGCACAA CAGCTACGAA
    GATGCCAAGG CCTATGGCTT CAAGAACAAG CTGATCATCG TCTCAGCGGA GACGGCGGGC
    AACGGGCTGT ACAACTTCAT CGTGCCGCTG CGCGCCTACT ACCGGCCTCG CCGGGAGCTC
    AACCCCATTG TGCTGCTGCT GGACAACAAG CCCGACCACC ACTTCCTGGA GGCCATCTGC
    TGCTTTCCCA TGGTCTACTA CATGGAGGGC TCCGTGGACA ACCTGGACAG CCTGCTGCGG
    TGCGGTGTCA TCTACGCGGA CAATCTGGTG GTGGCAGACA AGGAGAGCAC CATGAGCGCT
    GAGGAGGACT ACATGGCCGA CGCAAAGACC ATCGTCAATG TGCAGACCAT GTTCCGGCTC
    TTCCCCAGTC TCAGCATCAC CACCGAGCTC ACCCACCCGT CCAACATGCG GTTCATGCAG
    TTTCGCGCCA AGGACAGCTA CTCTCTGGCT CTCTCTAAAC TGGAGAAGAG GGAGCGTGAG
    AACGGCTCCA ACCTGGCCTT CATGTTCCGC CTGCCCTTCG CCGCCGGCCG CGTCTTCAGC
    ATCAGCATGC TGGACACACT GCTGTATCAG TCCTTCGTGA AGGACTACAT GATCCCCATC
    ACCAGGCTGT TGCTGGGCTT GGACACCACG CCGGGCTCCG GCTACCTCTG TGCGATGAAG
    ATCTCGGAGG ACGACCTGTG GATCCGCACC TATGGCCGCC TCTTCCAGAA GCTGTGCTCC
    TCCAGCGCCG AGATCCCTAT CGGCATCTAC AGGACCCAGT GCCACGTCTT CTCCACCGAG
    CCCCCCGACC TCAGGGCCCA GGCCTCACCC CTCCTCCCCG GCCAGTCCCA GGTCTCGGTG
    AGCGTGGAGG ACTGTGAGGA CACTCGGGAG GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC
    ACGAGCGGCG GCAGCACCCA CGGCCGGCAC ACGGTGGGCA GCGACCCAGC CGAGCACCCG
    CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC
    ACGGGGAATG CGGCAGCCGA GTGGATCAGC CAACAGCGGC TCAGCCTGTA CCGGCGCTCC
    GAGAGGCAGG AGCTCTCAGA GCTGGTCAAG AACCGCATGA AGCACCTGGG GCTGCCCACC
    AGTGGCTACG AGGATGTAGC AAATTTAACA GCCAGTGATG TCATGAATCG GGTAAACCTG
    GGATATTTGC AAGACGAGAT GAATGACCAC CAGAACACCC TCTCCTATGT GCTCATCAAC
    CCCCCGCCCG ACACGCGGCT GGAGCCCAAT GACATTGTAT ATCTCATCCG CTCCGACCCC
    TTGGCCCACG TGGCCAGCAG CTCCCAGAGC CGGAAGAGCA GCTGCAGCAA CAAGCTGGCC
    TCCTGCAACC CTGAGACTCG GGACGAGACA CAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92030
    Clone ID Related Accession (Same CDS sequence) XM_015465529.1 , XM_015473693.1
    Accession Version XM_015465529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC
    GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG
    CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC
    AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG
    AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG
    CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG
    AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT
    GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC
    CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA
    TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC
    AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321015.1
    CDS1588..5127
    Translation

    Target ORF information:

    RefSeq Version XM_015465529.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC
    GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG
    CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC
    AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG
    AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG
    CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG
    AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT
    GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC
    CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA
    TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC
    AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92030
    Clone ID Related Accession (Same CDS sequence) XM_015465529.1 , XM_015473693.1
    Accession Version XM_015473693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product potassium channel subfamily T member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC
    GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG
    CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC
    AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG
    AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG
    CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG
    AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT
    GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC
    CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA
    TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC
    AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329179.1
    CDS1588..5127
    Translation

    Target ORF information:

    RefSeq Version XM_015473693.1
    Organism Bos taurus(cattle)
    Definition Bos taurus potassium channel, sodium activated subfamily T, member 1 (KCNT1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTGTTC 
    TTCATCAAAA ACCAAAGATC CAGCCTGCGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG
    CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATCGGA
    TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCCGA GATCAACTGG
    GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC
    ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG
    GAGCAGATCT TCCGCGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC
    ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG
    GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GTGCCATCCT GCGCTCGCAG
    TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCT TGCTGTGCCT GGTCTTCACA
    GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACATCC
    TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC GCCCAAGATC
    TGGCCGTCCC AGCTGCTGGT GGTCATCATG ATCTGTGTGG CCCTGGTGGT GCTCCCGCTG
    CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC
    CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC
    CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GATTGCAGGA CTACTACGTC
    GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG
    TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG
    GCCAAGATGG ACAATGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC
    ACCGCTGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCAAAC
    TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG
    CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA
    GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC
    TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG
    CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC
    TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAATAAG
    AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC
    ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG
    AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC GGTGCACAGC
    ATCATTGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ATACGGAGTG CCGACCCACA
    CAGAGTGGCG GGGGCAGTGG GGGCAGCAAG CTGGCGCTCC CCACGGAGAA CGGCTCAGGC
    AGCCGGCGGC CCAGCATCGC GCCGGTGCTG GAGGTGGCTG ACAGCTCAGC CCTGCTGCCC
    TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA TGAGGGGCTG
    TCCGTGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCA
    ACCCTGTGCC ATCTCCTGCC CGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC
    TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTATGGCT TCAAGAACAA GCTGATCATC
    GTCTCAGCGG AGACGGCGGG CAACGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC
    TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC
    CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC
    AACCTGGACA GCCTGCTGCG GTGCGGTGTC ATCTACGCGG ACAATCTGGT GGTGGCAGAC
    AAGGAGAGCA CCATGAGCGC TGAGGAGGAC TACATGGCCG ACGCAAAGAC CATCGTCAAT
    GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG
    TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA
    CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC
    GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG
    AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCC
    GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTATGGCCGC
    CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAG
    TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC
    GGCCAGTCCC AGGTCTCGGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG
    CCCTGGGGTG CACGGGTGGG CACGAGCGGC GGCAGCACCC ACGGCCGGCA CACGGTGGGC
    AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG
    AGCCGCAAGG GCTCCCGGCA CACGGGGAAT GCGGCAGCCG AGTGGATCAG CCAACAGCGG
    CTCAGCCTGT ACCGGCGCTC CGAGAGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG
    AAGCACCTGG GGCTGCCCAC CAGTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT
    GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC
    CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA
    TATCTCATCC GCTCCGACCC CTTGGCCCAC GTGGCCAGCA GCTCCCAGAG CCGGAAGAGC
    AGCTGCAGCA ACAAGCTGGC CTCCTGCAAC CCTGAGACTC GGGACGAGAC ACAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.