RHBDF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015458397.1	XP_015313883.1	inactive rhomboid protein 1 isoform X1
XM_010800791.2	XP_010799093.1	inactive rhomboid protein 1 isoform X1
XM_015458447.2	XP_015313933.1	inactive rhomboid protein 1 isoform X1
XM_010800791.3	XP_010799093.1	inactive rhomboid protein 1 isoform X1
NM_001105400.1	NP_001098870.1	inactive rhomboid protein 1
XM_010800790.1	XP_010799092.1	inactive rhomboid protein 1 isoform X1
XM_010800790.2	XP_010799092.1	inactive rhomboid protein 1 isoform X1
XM_024984958.1	XP_024840726.1	inactive rhomboid protein 1 isoform X2
XM_015458447.1	XP_015313933.1	inactive rhomboid protein 1 isoform X1
XM_015458497.1	XP_015313983.1	inactive rhomboid protein 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa15354	NM_001105400.1 Latest version!	Bos taurus rhomboid 5 homolog 1 (RHBDF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa15354	XM_015458397.1 Latest version!	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa15354	XM_015458447.2 Latest version!	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa15354	XM_010800790.2 Latest version!	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa15354	XM_010800791.3 Latest version!	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa88386	XM_024984958.1 Latest version!	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa88386	XM_015458497.1 Latest version!	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa15354	XM_010800791.2	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa15354	XM_010800790.1	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa15354	XM_015458447.1	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

RHBDF1 ( NM_001105400.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001098870.1 Bos taurus rhomboid 5 homolog 1 (RHBDF1), mRNA.

Price & Availability↑

CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	NM_001105400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134588.1. On Sep 25, 2007 this sequence version replaced XM_607607.3. ##Evidence-Data-START## Transcript exon combination :: BC134588.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098870.1
CDS	46..2616
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	NM_001105400.1
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (RHBDF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105400.1

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_015458397.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313883.1 Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	XM_015458397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003087167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313883.1
CDS	316..2886
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_015458397.1
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458397.1

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_010800791.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799093.1 Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA.

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CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	XM_010800791.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003087167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799093.1
CDS	3324..5894
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_010800791.2
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800791.2

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_015458447.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313933.1 Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	XM_015458447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313933.1
CDS	3202..5772
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_015458447.2
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458447.2

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_010800791.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799093.1 Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	XM_010800791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799093.1
CDS	133..2703
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_010800791.3
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800791.3

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_010800790.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799092.1 Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	XM_010800790.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003087167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799092.1
CDS	219..2789
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_010800790.1
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800790.1

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_010800790.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799092.1 Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

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CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	XM_010800790.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799092.1
CDS	219..2789
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_010800790.2
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800790.2

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_015458447.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313933.1 Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA.

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CloneID	OBa15354
Clone ID Related Accession (Same CDS sequence)	XM_015458397.1 , XM_010800791.2 , XM_015458447.2 , XM_010800791.3 , NM_001105400.1 , XM_010800790.1 , XM_010800790.2 , XM_015458447.1
Accession Version	XM_015458447.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003087167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313933.1
CDS	3208..5778
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAEPARVPSPHQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_015458447.1
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458447.1

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGCCC 
TTGAGACGCC AGGCATTCCT GCGGAGCGTG AGCATGCCGG CCGAGCCCGC CCGCGTCCCT 
TCGCCCCACC AGGAGCCCCG GCGGCCGGTG CTACAGCGCC AGATGTCCAT CACACAGACC 
ATCCGCAGGG GCACGGCCGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGCAGC CAGCGCTACG GGAAGCTGAA GCCCCAGGTC 
ATCCGCGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCGC TGACCAGCAC TGAGACGCCG 
CCTCCTCTGT ACGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCGTAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCTT GGCGGATGAT GCCGCCGACG GCCCGAGCGC CCCGCACACG 
CCCGTCACGC CGGGTGCCGC CTCCCTCTGC TCCTTCTCCA GCTCCCGCTC CGGTTTCAAC 
CGGCTCCCGC GGCGGCGCAA GCGCGAGTCG GTGGCCAAGA TGAGCTTCCG GGCAGCTGCT 
GCGCTGGTGA AGGGTCGCTC GGTTAGGGAT GGCACGTTAC GCCGCGCCCA GCGCCGAAGC 
TTCACTCCTG CCAGCTTCCT GGAAGAGGAC ACGGCTGACT TCCCTGACGA GCTGGACACG 
TCCTTCTTTG CTCGGGAAGG TGTCCTCCAC GAGGAGCTGT CCACTTACCC GGACGAGGTG 
TTCGAGTCCC CATCGGAGGC AGCGCTTAAA GACTGGGAGC GAGCCCCAGA GCAGGTGGAC 
CTCACGGGCG GCGCCCTGGA CCGCAGCGAG CTGGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGTGGCT GGCGCAAGCA GAAGGAGGGC GGCGCAGCGG CCCCGCAGCC CAAGGTGCGG 
CTGCGGCAGG AGGTGGTGAG CACGGCGGGG CAGCGGCGGG GCCAGCGCAT CGCGATGCCG 
GTGCGCAAGT TGTTTGCCAG GGAGAAGCGG CCGTACGGGC TGGGCATGGT GGGCCGGCTA 
ACCAACCGCA CTTACCGCAA GCGAATAGAC AGCTATGTCA AACGCCAGAT TGAGGACATG 
GACGACCACA GGCCCTTCTT CACCTACTGG CTGACCTTCG TGCACTCGCT CGTCACCATC 
CTAGCCGTGT GCATCTATGG CGTGGCGCCC GTGGGCTTCT CACAGCACGA GACCGTAGAC 
TCGGTGCTGC GCAACCGTGG GGTCTACGAG AATGTCAAGT ACGTGCAACA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCCCTCATT CACCTGGGTG CCAAGTTCTC GCCGTGCATG 
CGCCAGGACC CGCAGGTGCA TAGTTTCATC CATGCTGCGC GCGAGCGCGA GAAGCACTCG 
GCCTGCTGCG TGCGTAACGA CCGGTCAGGC TGCGTGCAGA CCTCGGAGGA GGAGTGCTCG 
TCCACGCTGG CAGTGTGGGT GAAGTGGCCC CTGCATCCCA GTGCCCCAGA CCTTGCTGGC 
CAAAAGAGGC GGTACGGCTC TGTCTGCCAC CAGGATCCCA GGGTGTGTGA TGAGCCTTCT 
TCTGAGGACC CCCATGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAGC 
AGTGCTGGGA ACCACACCAA CCACCCGCAC ATGGACTGCG TCATCACGGG CCGGCCCTGC 
TGCATCGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGTTACTTCC ACGAGGAGGC CACACTCTGC TCCCAGGTGC ACTGCATGGA CGACGTGTGT 
GGGCTCCTGC CCTTCCTCAA CCCCGAGGTG CCTGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGGT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACAGTC 
CTGCGAGACC TGGAGAAGCT GGCGGGCTGG CACCGCATAG CCATCATCTA CCTGCTCAGT 
GGCGTCACTG GTAACCTGGC TAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCTGGCTCGC AGTTTGGCAT CCTGGCCTGC CTCTTCGTGG AGCTCTTCCA GAGCTGGCAG 
ATTCTGGCGC GGCCCTGGCG CGCCTTCTTC AAGCTGCTGG CCGTGGTGCT CTTCCTCTTC 
ACCTTCGGCC TGCTGCCCTG GATCGACAAC TTCGCCCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTCT CCTTTGCCTT CCTGCCCTAC ATTAGCTTCG GCAAGTTCGA CCTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTTCAGCTG GTCTTCCTGG GCCTGCTGGC TGGCCTGGTG 
GTCCTCTTCT ACTTCTACCC TGTCCGCTGC GAGTGGTGTG AGTTCCTCAC CTGCATCCCC 
TTCACTGACA AGTTCTGTGA GAAGTACGAG CTGGATGCTC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_024984958.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840726.1 Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

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CloneID	OBa88386
Clone ID Related Accession (Same CDS sequence)	XM_024984958.1 , XM_015458497.1
Accession Version	XM_024984958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGGAG 
CCCCGGCGGC CGGTGCTACA GCGCCAGATG TCCATCACAC AGACCATCCG CAGGGGCACG 
GCCGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GCAGCCAGCG CTACGGGAAG CTGAAGCCCC AGGTCATCCG CGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCGCTGACC AGCACTGAGA CGCCGCCTCC TCTGTACGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC GTAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCTTGGCGG ATGATGCCGC CGACGGCCCG AGCGCCCCGC ACACGCCCGT CACGCCGGGT 
GCCGCCTCCC TCTGCTCCTT CTCCAGCTCC CGCTCCGGTT TCAACCGGCT CCCGCGGCGG 
CGCAAGCGCG AGTCGGTGGC CAAGATGAGC TTCCGGGCAG CTGCTGCGCT GGTGAAGGGT 
CGCTCGGTTA GGGATGGCAC GTTACGCCGC GCCCAGCGCC GAAGCTTCAC TCCTGCCAGC 
TTCCTGGAAG AGGACACGGC TGACTTCCCT GACGAGCTGG ACACGTCCTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GCTGTCCACT TACCCGGACG AGGTGTTCGA GTCCCCATCG 
GAGGCAGCGC TTAAAGACTG GGAGCGAGCC CCAGAGCAGG TGGACCTCAC GGGCGGCGCC 
CTGGACCGCA GCGAGCTGGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG TGGCTGGCGC 
AAGCAGAAGG AGGGCGGCGC AGCGGCCCCG CAGCCCAAGG TGCGGCTGCG GCAGGAGGTG 
GTGAGCACGG CGGGGCAGCG GCGGGGCCAG CGCATCGCGA TGCCGGTGCG CAAGTTGTTT 
GCCAGGGAGA AGCGGCCGTA CGGGCTGGGC ATGGTGGGCC GGCTAACCAA CCGCACTTAC 
CGCAAGCGAA TAGACAGCTA TGTCAAACGC CAGATTGAGG ACATGGACGA CCACAGGCCC 
TTCTTCACCT ACTGGCTGAC CTTCGTGCAC TCGCTCGTCA CCATCCTAGC CGTGTGCATC 
TATGGCGTGG CGCCCGTGGG CTTCTCACAG CACGAGACCG TAGACTCGGT GCTGCGCAAC 
CGTGGGGTCT ACGAGAATGT CAAGTACGTG CAACAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCCC TCATTCACCT GGGTGCCAAG TTCTCGCCGT GCATGCGCCA GGACCCGCAG 
GTGCATAGTT TCATCCATGC TGCGCGCGAG CGCGAGAAGC ACTCGGCCTG CTGCGTGCGT 
AACGACCGGT CAGGCTGCGT GCAGACCTCG GAGGAGGAGT GCTCGTCCAC GCTGGCAGTG 
TGGGTGAAGT GGCCCCTGCA TCCCAGTGCC CCAGACCTTG CTGGCCAAAA GAGGCGGTAC 
GGCTCTGTCT GCCACCAGGA TCCCAGGGTG TGTGATGAGC CTTCTTCTGA GGACCCCCAT 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGTGC TGGGAACCAC 
ACCAACCACC CGCACATGGA CTGCGTCATC ACGGGCCGGC CCTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGTTA CTTCCACGAG 
GAGGCCACAC TCTGCTCCCA GGTGCACTGC ATGGACGACG TGTGTGGGCT CCTGCCCTTC 
CTCAACCCCG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGGTCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CAGTCCTGCG AGACCTGGAG 
AAGCTGGCGG GCTGGCACCG CATAGCCATC ATCTACCTGC TCAGTGGCGT CACTGGTAAC 
CTGGCTAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCTGG CTCGCAGTTT 
GGCATCCTGG CCTGCCTCTT CGTGGAGCTC TTCCAGAGCT GGCAGATTCT GGCGCGGCCC 
TGGCGCGCCT TCTTCAAGCT GCTGGCCGTG GTGCTCTTCC TCTTCACCTT CGGCCTGCTG 
CCCTGGATCG ACAACTTCGC CCACATCTCA GGCTTCATCA GCGGCCTCTT CCTCTCCTTT 
GCCTTCCTGC CCTACATTAG CTTCGGCAAG TTCGACCTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTTC AGCTGGTCTT CCTGGGCCTG CTGGCTGGCC TGGTGGTCCT CTTCTACTTC 
TACCCTGTCC GCTGCGAGTG GTGTGAGTTC CTCACCTGCA TCCCCTTCAC TGACAAGTTC 
TGTGAGAAGT ACGAGCTGGA TGCTCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840726.1
CDS	215..2710
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_024984958.1
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984958.1

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGGAG 
CCCCGGCGGC CGGTGCTACA GCGCCAGATG TCCATCACAC AGACCATCCG CAGGGGCACG 
GCCGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GCAGCCAGCG CTACGGGAAG CTGAAGCCCC AGGTCATCCG CGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCGCTGACC AGCACTGAGA CGCCGCCTCC TCTGTACGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC GTAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCTTGGCGG ATGATGCCGC CGACGGCCCG AGCGCCCCGC ACACGCCCGT CACGCCGGGT 
GCCGCCTCCC TCTGCTCCTT CTCCAGCTCC CGCTCCGGTT TCAACCGGCT CCCGCGGCGG 
CGCAAGCGCG AGTCGGTGGC CAAGATGAGC TTCCGGGCAG CTGCTGCGCT GGTGAAGGGT 
CGCTCGGTTA GGGATGGCAC GTTACGCCGC GCCCAGCGCC GAAGCTTCAC TCCTGCCAGC 
TTCCTGGAAG AGGACACGGC TGACTTCCCT GACGAGCTGG ACACGTCCTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GCTGTCCACT TACCCGGACG AGGTGTTCGA GTCCCCATCG 
GAGGCAGCGC TTAAAGACTG GGAGCGAGCC CCAGAGCAGG TGGACCTCAC GGGCGGCGCC 
CTGGACCGCA GCGAGCTGGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG TGGCTGGCGC 
AAGCAGAAGG AGGGCGGCGC AGCGGCCCCG CAGCCCAAGG TGCGGCTGCG GCAGGAGGTG 
GTGAGCACGG CGGGGCAGCG GCGGGGCCAG CGCATCGCGA TGCCGGTGCG CAAGTTGTTT 
GCCAGGGAGA AGCGGCCGTA CGGGCTGGGC ATGGTGGGCC GGCTAACCAA CCGCACTTAC 
CGCAAGCGAA TAGACAGCTA TGTCAAACGC CAGATTGAGG ACATGGACGA CCACAGGCCC 
TTCTTCACCT ACTGGCTGAC CTTCGTGCAC TCGCTCGTCA CCATCCTAGC CGTGTGCATC 
TATGGCGTGG CGCCCGTGGG CTTCTCACAG CACGAGACCG TAGACTCGGT GCTGCGCAAC 
CGTGGGGTCT ACGAGAATGT CAAGTACGTG CAACAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCCC TCATTCACCT GGGTGCCAAG TTCTCGCCGT GCATGCGCCA GGACCCGCAG 
GTGCATAGTT TCATCCATGC TGCGCGCGAG CGCGAGAAGC ACTCGGCCTG CTGCGTGCGT 
AACGACCGGT CAGGCTGCGT GCAGACCTCG GAGGAGGAGT GCTCGTCCAC GCTGGCAGTG 
TGGGTGAAGT GGCCCCTGCA TCCCAGTGCC CCAGACCTTG CTGGCCAAAA GAGGCGGTAC 
GGCTCTGTCT GCCACCAGGA TCCCAGGGTG TGTGATGAGC CTTCTTCTGA GGACCCCCAT 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGTGC TGGGAACCAC 
ACCAACCACC CGCACATGGA CTGCGTCATC ACGGGCCGGC CCTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGTTA CTTCCACGAG 
GAGGCCACAC TCTGCTCCCA GGTGCACTGC ATGGACGACG TGTGTGGGCT CCTGCCCTTC 
CTCAACCCCG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGGTCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CAGTCCTGCG AGACCTGGAG 
AAGCTGGCGG GCTGGCACCG CATAGCCATC ATCTACCTGC TCAGTGGCGT CACTGGTAAC 
CTGGCTAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCTGG CTCGCAGTTT 
GGCATCCTGG CCTGCCTCTT CGTGGAGCTC TTCCAGAGCT GGCAGATTCT GGCGCGGCCC 
TGGCGCGCCT TCTTCAAGCT GCTGGCCGTG GTGCTCTTCC TCTTCACCTT CGGCCTGCTG 
CCCTGGATCG ACAACTTCGC CCACATCTCA GGCTTCATCA GCGGCCTCTT CCTCTCCTTT 
GCCTTCCTGC CCTACATTAG CTTCGGCAAG TTCGACCTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTTC AGCTGGTCTT CCTGGGCCTG CTGGCTGGCC TGGTGGTCCT CTTCTACTTC 
TACCCTGTCC GCTGCGAGTG GTGTGAGTTC CTCACCTGCA TCCCCTTCAC TGACAAGTTC 
TGTGAGAAGT ACGAGCTGGA TGCTCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_015458497.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313983.1 Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA.

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CloneID	OBa88386
Clone ID Related Accession (Same CDS sequence)	XM_024984958.1 , XM_015458497.1
Accession Version	XM_015458497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003087167.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGGAG 
CCCCGGCGGC CGGTGCTACA GCGCCAGATG TCCATCACAC AGACCATCCG CAGGGGCACG 
GCCGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GCAGCCAGCG CTACGGGAAG CTGAAGCCCC AGGTCATCCG CGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCGCTGACC AGCACTGAGA CGCCGCCTCC TCTGTACGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC GTAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCTTGGCGG ATGATGCCGC CGACGGCCCG AGCGCCCCGC ACACGCCCGT CACGCCGGGT 
GCCGCCTCCC TCTGCTCCTT CTCCAGCTCC CGCTCCGGTT TCAACCGGCT CCCGCGGCGG 
CGCAAGCGCG AGTCGGTGGC CAAGATGAGC TTCCGGGCAG CTGCTGCGCT GGTGAAGGGT 
CGCTCGGTTA GGGATGGCAC GTTACGCCGC GCCCAGCGCC GAAGCTTCAC TCCTGCCAGC 
TTCCTGGAAG AGGACACGGC TGACTTCCCT GACGAGCTGG ACACGTCCTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GCTGTCCACT TACCCGGACG AGGTGTTCGA GTCCCCATCG 
GAGGCAGCGC TTAAAGACTG GGAGCGAGCC CCAGAGCAGG TGGACCTCAC GGGCGGCGCC 
CTGGACCGCA GCGAGCTGGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG TGGCTGGCGC 
AAGCAGAAGG AGGGCGGCGC AGCGGCCCCG CAGCCCAAGG TGCGGCTGCG GCAGGAGGTG 
GTGAGCACGG CGGGGCAGCG GCGGGGCCAG CGCATCGCGA TGCCGGTGCG CAAGTTGTTT 
GCCAGGGAGA AGCGGCCGTA CGGGCTGGGC ATGGTGGGCC GGCTAACCAA CCGCACTTAC 
CGCAAGCGAA TAGACAGCTA TGTCAAACGC CAGATTGAGG ACATGGACGA CCACAGGCCC 
TTCTTCACCT ACTGGCTGAC CTTCGTGCAC TCGCTCGTCA CCATCCTAGC CGTGTGCATC 
TATGGCGTGG CGCCCGTGGG CTTCTCACAG CACGAGACCG TAGACTCGGT GCTGCGCAAC 
CGTGGGGTCT ACGAGAATGT CAAGTACGTG CAACAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCCC TCATTCACCT GGGTGCCAAG TTCTCGCCGT GCATGCGCCA GGACCCGCAG 
GTGCATAGTT TCATCCATGC TGCGCGCGAG CGCGAGAAGC ACTCGGCCTG CTGCGTGCGT 
AACGACCGGT CAGGCTGCGT GCAGACCTCG GAGGAGGAGT GCTCGTCCAC GCTGGCAGTG 
TGGGTGAAGT GGCCCCTGCA TCCCAGTGCC CCAGACCTTG CTGGCCAAAA GAGGCGGTAC 
GGCTCTGTCT GCCACCAGGA TCCCAGGGTG TGTGATGAGC CTTCTTCTGA GGACCCCCAT 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGTGC TGGGAACCAC 
ACCAACCACC CGCACATGGA CTGCGTCATC ACGGGCCGGC CCTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGTTA CTTCCACGAG 
GAGGCCACAC TCTGCTCCCA GGTGCACTGC ATGGACGACG TGTGTGGGCT CCTGCCCTTC 
CTCAACCCCG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGGTCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CAGTCCTGCG AGACCTGGAG 
AAGCTGGCGG GCTGGCACCG CATAGCCATC ATCTACCTGC TCAGTGGCGT CACTGGTAAC 
CTGGCTAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCTGG CTCGCAGTTT 
GGCATCCTGG CCTGCCTCTT CGTGGAGCTC TTCCAGAGCT GGCAGATTCT GGCGCGGCCC 
TGGCGCGCCT TCTTCAAGCT GCTGGCCGTG GTGCTCTTCC TCTTCACCTT CGGCCTGCTG 
CCCTGGATCG ACAACTTCGC CCACATCTCA GGCTTCATCA GCGGCCTCTT CCTCTCCTTT 
GCCTTCCTGC CCTACATTAG CTTCGGCAAG TTCGACCTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTTC AGCTGGTCTT CCTGGGCCTG CTGGCTGGCC TGGTGGTCCT CTTCTACTTC 
TACCCTGTCC GCTGCGAGTG GTGTGAGTTC CTCACCTGCA TCCCCTTCAC TGACAAGTTC 
TGTGAGAAGT ACGAGCTGGA TGCTCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313983.1
CDS	316..2811
Translation	MGEARRDSSSSLQHKKPPWLKLDIPAVVPPAAEEPSFLQEPRRPVLQRQMSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIVDPLARGRAFRLADDAADGPSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRAQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWERAPEQVDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAAPQPKVRLRQEVVSTAGQRRGQRIAMPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGVAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIHAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGQKRRYGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKSSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGVLHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQLVFLGLLAGLVVLFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_015458497.1
Organism	Bos taurus(cattle)
Definition	Bos taurus rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458497.1

ORF Insert Sequence:

ATGGGCGAGG CCCGCAGGGA CAGCAGCAGC AGCCTGCAGC ACAAGAAGCC GCCGTGGCTG 
AAGCTGGACA TCCCGGCCGT GGTGCCCCCG GCAGCAGAGG AGCCCAGCTT CCTGCAGGAG 
CCCCGGCGGC CGGTGCTACA GCGCCAGATG TCCATCACAC AGACCATCCG CAGGGGCACG 
GCCGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GCAGCCAGCG CTACGGGAAG CTGAAGCCCC AGGTCATCCG CGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCGCTGACC AGCACTGAGA CGCCGCCTCC TCTGTACGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC GTAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCTTGGCGG ATGATGCCGC CGACGGCCCG AGCGCCCCGC ACACGCCCGT CACGCCGGGT 
GCCGCCTCCC TCTGCTCCTT CTCCAGCTCC CGCTCCGGTT TCAACCGGCT CCCGCGGCGG 
CGCAAGCGCG AGTCGGTGGC CAAGATGAGC TTCCGGGCAG CTGCTGCGCT GGTGAAGGGT 
CGCTCGGTTA GGGATGGCAC GTTACGCCGC GCCCAGCGCC GAAGCTTCAC TCCTGCCAGC 
TTCCTGGAAG AGGACACGGC TGACTTCCCT GACGAGCTGG ACACGTCCTT CTTTGCTCGG 
GAAGGTGTCC TCCACGAGGA GCTGTCCACT TACCCGGACG AGGTGTTCGA GTCCCCATCG 
GAGGCAGCGC TTAAAGACTG GGAGCGAGCC CCAGAGCAGG TGGACCTCAC GGGCGGCGCC 
CTGGACCGCA GCGAGCTGGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG TGGCTGGCGC 
AAGCAGAAGG AGGGCGGCGC AGCGGCCCCG CAGCCCAAGG TGCGGCTGCG GCAGGAGGTG 
GTGAGCACGG CGGGGCAGCG GCGGGGCCAG CGCATCGCGA TGCCGGTGCG CAAGTTGTTT 
GCCAGGGAGA AGCGGCCGTA CGGGCTGGGC ATGGTGGGCC GGCTAACCAA CCGCACTTAC 
CGCAAGCGAA TAGACAGCTA TGTCAAACGC CAGATTGAGG ACATGGACGA CCACAGGCCC 
TTCTTCACCT ACTGGCTGAC CTTCGTGCAC TCGCTCGTCA CCATCCTAGC CGTGTGCATC 
TATGGCGTGG CGCCCGTGGG CTTCTCACAG CACGAGACCG TAGACTCGGT GCTGCGCAAC 
CGTGGGGTCT ACGAGAATGT CAAGTACGTG CAACAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCCC TCATTCACCT GGGTGCCAAG TTCTCGCCGT GCATGCGCCA GGACCCGCAG 
GTGCATAGTT TCATCCATGC TGCGCGCGAG CGCGAGAAGC ACTCGGCCTG CTGCGTGCGT 
AACGACCGGT CAGGCTGCGT GCAGACCTCG GAGGAGGAGT GCTCGTCCAC GCTGGCAGTG 
TGGGTGAAGT GGCCCCTGCA TCCCAGTGCC CCAGACCTTG CTGGCCAAAA GAGGCGGTAC 
GGCTCTGTCT GCCACCAGGA TCCCAGGGTG TGTGATGAGC CTTCTTCTGA GGACCCCCAT 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAGCAGTGC TGGGAACCAC 
ACCAACCACC CGCACATGGA CTGCGTCATC ACGGGCCGGC CCTGCTGCAT CGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGTTA CTTCCACGAG 
GAGGCCACAC TCTGCTCCCA GGTGCACTGC ATGGACGACG TGTGTGGGCT CCTGCCCTTC 
CTCAACCCCG AGGTGCCTGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGGTCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CAGTCCTGCG AGACCTGGAG 
AAGCTGGCGG GCTGGCACCG CATAGCCATC ATCTACCTGC TCAGTGGCGT CACTGGTAAC 
CTGGCTAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCTGG CTCGCAGTTT 
GGCATCCTGG CCTGCCTCTT CGTGGAGCTC TTCCAGAGCT GGCAGATTCT GGCGCGGCCC 
TGGCGCGCCT TCTTCAAGCT GCTGGCCGTG GTGCTCTTCC TCTTCACCTT CGGCCTGCTG 
CCCTGGATCG ACAACTTCGC CCACATCTCA GGCTTCATCA GCGGCCTCTT CCTCTCCTTT 
GCCTTCCTGC CCTACATTAG CTTCGGCAAG TTCGACCTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTTC AGCTGGTCTT CCTGGGCCTG CTGGCTGGCC TGGTGGTCCT CTTCTACTTC 
TACCCTGTCC GCTGCGAGTG GTGTGAGTTC CTCACCTGCA TCCCCTTCAC TGACAAGTTC 
TGTGAGAAGT ACGAGCTGGA TGCTCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RHBDF1
Entrez Gene ID	529167
Full Name	rhomboid 5 homolog 1
General protein information	Preferred Names rhomboid 5 homolog 1 Names inactive rhomboid protein 1 Rhomboid family member 1 iRhom1 rhomboid family 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	RHBDF1	XP_510701.3
Rattus norvegicus (Norway rat)	Rhbdf1	NP_001025205.1
Danio rerio (zebrafish)	rhbdf1	NP_001002228.1
Xenopus tropicalis (tropical clawed frog)	rhbdf1	XP_004917998.1
Homo sapiens (human)	RHBDF1	NP_071895.3
Gallus gallus (chicken)	RHBDF1	XP_004945406.1
Canis lupus familiaris (dog)	RHBDF1	XP_005621827.1
Bos taurus (cattle)	RHBDF1	NP_001098870.1
Mus musculus (house mouse)	Rhbdf1	NP_034247.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RHBDF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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