MED14 cDNA ORF clone, Bos taurus(cattle)

The following MED14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238416 XM_024988080.1
Latest version!
Bos taurus mediator complex subunit 14 (MED14), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa38551 NM_001207031.1
Latest version!
Bos taurus mediator complex subunit 14 (MED14), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa35685 XM_005228292.4
Latest version!
Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa35686 XM_005228293.4
Latest version!
Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa35686 XM_005228293.3 Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa35685 XM_005228292.3 Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238416
    Clone ID Related Accession (Same CDS sequence) XM_024988080.1
    Accession Version XM_024988080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 14 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC
    TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG
    CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA
    GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAACCCTGGA CCCTAGTTCT
    CCATACACTA TGGTGTCACC AAGTGGCCGA GCAGGGAACT GGCCAGGGTC TCCTCAAGTG
    TCTGGCCCCT CACCAGCAAC ACGCATGCCT GGGATGTCAC CAGCCAACCC ATCACTACAT
    TCTCCAGTTC CAGATGCTTC TCATTCCCCT CGAGCTGGAA CAAGTTCCCA AACGATGCCA
    ACAAACATGC CTCCACCTCG TAAACTACCT CAGCGCTCCT GGGCAGCCTC CATCCCTACC
    ATCCTCACTC ACAGTGCCTT GAACATTTTG CTGCTGCCCT CTCCAACGCC AGGCCTCGTG
    CCCGGCCTGG CAGGTAGTTA CCTATGTTCT CCACTTGAGA GATTCCTTGG ATCAGTCATC
    ATGAGACGCC ACCTTCAAAG AATTATTCAG CAGGAGACGC TGCAGCTGAT AAATTCCAAT
    GAACCCGGGG TGATCATGTT TAAGACTGAT GCACTGAAAT GCAGAGTAGC CCTTAGTCCT
    AAAACCAACC AAACACTTCA GCTAAAAGTG ACACCCGAAA ATGCAGGACA GTGGAAACCT
    GATGAACTTC AAGTTTTGGA GAAATTCTTT GAAACAAGAG TTGCAGGACC ACCATTTAAA
    GCCAATACAT TAATAGCTTT CACCAAGCTG TTAGGAGCTC CTACACACAT CCTCAGGGAC
    TGTGTGCATA TTATGAAGCT AGAGCTGTTT CCTGACCAGG CAACACAGCT AAAATGGAAT
    GTTCAGTTTT GCCTGACGAT CCCTCCCAGT GCACCACCAA TTGCACCTCC TGGGACACCT
    GCTGTGGTGC TGAAATCCAA AATGCTATTT TTTCTTCAGC TGACTCAGAA GACATCAGTC
    CCTCCCCAAG AACCTGTTAG TATTATAGTT CCAATCATTT ATGACATGGC TTCAGGTACC
    ACCCAGCAGG CAGACATTCC CAGGCAGCAG AACTCTTCTG TTGCTGCTCC CATGATGGTC
    AGCAACATTC TGAAGAGGTT TGCAGAGATG AACCCACCAC GACAAGGTGA ATGCACAATA
    TTTGCAGCTG TTCGTGATTT AATGGCTAAT CTTACACTGC CCCCTGGTGG GCGTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843848.1
    CDS338..4537
    Translation

    Target ORF information:

    RefSeq Version XM_024988080.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC
    TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG
    CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA
    GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAACCCTGGA CCCTAGTTCT
    CCATACACTA TGGTGTCACC AAGTGGCCGA GCAGGGAACT GGCCAGGGTC TCCTCAAGTG
    TCTGGCCCCT CACCAGCAAC ACGCATGCCT GGGATGTCAC CAGCCAACCC ATCACTACAT
    TCTCCAGTTC CAGATGCTTC TCATTCCCCT CGAGCTGGAA CAAGTTCCCA AACGATGCCA
    ACAAACATGC CTCCACCTCG TAAACTACCT CAGCGCTCCT GGGCAGCCTC CATCCCTACC
    ATCCTCACTC ACAGTGCCTT GAACATTTTG CTGCTGCCCT CTCCAACGCC AGGCCTCGTG
    CCCGGCCTGG CAGGTAGTTA CCTATGTTCT CCACTTGAGA GATTCCTTGG ATCAGTCATC
    ATGAGACGCC ACCTTCAAAG AATTATTCAG CAGGAGACGC TGCAGCTGAT AAATTCCAAT
    GAACCCGGGG TGATCATGTT TAAGACTGAT GCACTGAAAT GCAGAGTAGC CCTTAGTCCT
    AAAACCAACC AAACACTTCA GCTAAAAGTG ACACCCGAAA ATGCAGGACA GTGGAAACCT
    GATGAACTTC AAGTTTTGGA GAAATTCTTT GAAACAAGAG TTGCAGGACC ACCATTTAAA
    GCCAATACAT TAATAGCTTT CACCAAGCTG TTAGGAGCTC CTACACACAT CCTCAGGGAC
    TGTGTGCATA TTATGAAGCT AGAGCTGTTT CCTGACCAGG CAACACAGCT AAAATGGAAT
    GTTCAGTTTT GCCTGACGAT CCCTCCCAGT GCACCACCAA TTGCACCTCC TGGGACACCT
    GCTGTGGTGC TGAAATCCAA AATGCTATTT TTTCTTCAGC TGACTCAGAA GACATCAGTC
    CCTCCCCAAG AACCTGTTAG TATTATAGTT CCAATCATTT ATGACATGGC TTCAGGTACC
    ACCCAGCAGG CAGACATTCC CAGGCAGCAG AACTCTTCTG TTGCTGCTCC CATGATGGTC
    AGCAACATTC TGAAGAGGTT TGCAGAGATG AACCCACCAC GACAAGGTGA ATGCACAATA
    TTTGCAGCTG TTCGTGATTT AATGGCTAAT CTTACACTGC CCCCTGGTGG GCGTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38551
    Clone ID Related Accession (Same CDS sequence) NM_001207031.1
    Accession Version NM_001207031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-02
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 14
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000030.1. On or before May 19, 2011 this sequence version replaced XR_084733.1, XR_083551.1. ##Evidence-Data-START## Transcript exon combination :: SRR5721695.5559.1, SRR5721696.3157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT
    AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA
    CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT
    TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG
    TCTCCGGGAA ATCTGCATGC TGCCAGCTCC CCGAGTGGGG CTTTGAGAGC CCCATCACCA
    GCATCATTTG TTCCAACTCC TCCCCCATCC TCGCATGGAA TCTCAATAGG ACCAGGGGCC
    AGTTTTGCTA GTCCACATGG AACCCTGGAC CCTAGTTCTC CATACACTAT GGTGTCACCA
    AGTGGCCGAG CAGGGAACTG GCCAGGGTCT CCTCAAGTGT CTGGCCCCTC ACCAGCAACA
    CGCATGCCTG GGATGTCACC AGCCAACCCA TCACTACATT CTCCAGTTCC AGATGCTTCT
    CATTCCCCTC GAGCTGGAAC AAGTTCCCAA ACGATGCCAA CAAACATGCC TCCACCTCGT
    AAACTACCTC AGCGCTCCTG GGCAGCCTCC ATCCCTACCA TCCTCACTCA CAGTGCCTTG
    AACATTTTGC TGCTGCCCTC TCCAACGCCA GGCCTCGTGC CCGGCCTGGC AGGTAGTTAC
    CTATGTTCTC CACTTGAGAG ATTCCTTGGA TCAGTCATCA TGAGACGCCA CCTTCAAAGA
    ATTATTCAGC AGGAGACGCT GCAGCTGATA AATTCCAATG AACCCGGGGT GATCATGTTT
    AAGACTGATG CACTGAAATG CAGAGTAGCC CTTAGTCCTA AAACCAACCA AACACTTCAG
    CTAAAAGTGA CACCCGAAAA TGCAGGACAG TGGAAACCTG ATGAACTTCA AGTTTTGGAG
    AAATTCTTTG AAACAAGAGT TGCAGGACCA CCATTTAAAG CCAATACATT AATAGCTTTC
    ACCAAGCTGT TAGGAGCTCC TACACACATC CTCAGGGACT GTGTGCATAT TATGAAGCTA
    GAGCTGTTTC CTGACCAGGC AACACAGCTA AAATGGAATG TTCAGTTTTG CCTGACGATC
    CCTCCCAGTG CACCACCAAT TGCACCTCCT GGGACACCTG CTGTGGTGCT GAAATCCAAA
    ATGCTATTTT TTCTTCAGCT GACTCAGAAG ACATCAGTCC CTCCCCAAGA ACCTGTTAGT
    ATTATAGTTC CAATCATTTA TGACATGGCT TCAGGTACCA CCCAGCAGGC AGACATTCCC
    AGGCAGCAGA ACTCTTCTGT TGCTGCTCCC ATGATGGTCA GCAACATTCT GAAGAGGTTT
    GCAGAGATGA ACCCACCACG ACAAGGTGAA TGCACAATAT TTGCAGCTGT TCGTGATTTA
    ATGGCTAATC TTACACTGCC CCCTGGTGGG CGTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193960.1
    CDS109..4467
    Translation

    Target ORF information:

    RefSeq Version NM_001207031.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 14 (MED14), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001207031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT
    AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA
    CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT
    TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG
    TCTCCGGGAA ATCTGCATGC TGCCAGCTCC CCGAGTGGGG CTTTGAGAGC CCCATCACCA
    GCATCATTTG TTCCAACTCC TCCCCCATCC TCGCATGGAA TCTCAATAGG ACCAGGGGCC
    AGTTTTGCTA GTCCACATGG AACCCTGGAC CCTAGTTCTC CATACACTAT GGTGTCACCA
    AGTGGCCGAG CAGGGAACTG GCCAGGGTCT CCTCAAGTGT CTGGCCCCTC ACCAGCAACA
    CGCATGCCTG GGATGTCACC AGCCAACCCA TCACTACATT CTCCAGTTCC AGATGCTTCT
    CATTCCCCTC GAGCTGGAAC AAGTTCCCAA ACGATGCCAA CAAACATGCC TCCACCTCGT
    AAACTACCTC AGCGCTCCTG GGCAGCCTCC ATCCCTACCA TCCTCACTCA CAGTGCCTTG
    AACATTTTGC TGCTGCCCTC TCCAACGCCA GGCCTCGTGC CCGGCCTGGC AGGTAGTTAC
    CTATGTTCTC CACTTGAGAG ATTCCTTGGA TCAGTCATCA TGAGACGCCA CCTTCAAAGA
    ATTATTCAGC AGGAGACGCT GCAGCTGATA AATTCCAATG AACCCGGGGT GATCATGTTT
    AAGACTGATG CACTGAAATG CAGAGTAGCC CTTAGTCCTA AAACCAACCA AACACTTCAG
    CTAAAAGTGA CACCCGAAAA TGCAGGACAG TGGAAACCTG ATGAACTTCA AGTTTTGGAG
    AAATTCTTTG AAACAAGAGT TGCAGGACCA CCATTTAAAG CCAATACATT AATAGCTTTC
    ACCAAGCTGT TAGGAGCTCC TACACACATC CTCAGGGACT GTGTGCATAT TATGAAGCTA
    GAGCTGTTTC CTGACCAGGC AACACAGCTA AAATGGAATG TTCAGTTTTG CCTGACGATC
    CCTCCCAGTG CACCACCAAT TGCACCTCCT GGGACACCTG CTGTGGTGCT GAAATCCAAA
    ATGCTATTTT TTCTTCAGCT GACTCAGAAG ACATCAGTCC CTCCCCAAGA ACCTGTTAGT
    ATTATAGTTC CAATCATTTA TGACATGGCT TCAGGTACCA CCCAGCAGGC AGACATTCCC
    AGGCAGCAGA ACTCTTCTGT TGCTGCTCCC ATGATGGTCA GCAACATTCT GAAGAGGTTT
    GCAGAGATGA ACCCACCACG ACAAGGTGAA TGCACAATAT TTGCAGCTGT TCGTGATTTA
    ATGGCTAATC TTACACTGCC CCCTGGTGGG CGTCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35685
    Clone ID Related Accession (Same CDS sequence) XM_005228292.4 , XM_005228292.3
    Accession Version XM_005228292.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 14 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228292.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC
    TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG
    CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA
    GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC
    TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA
    TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG
    GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG
    TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC
    CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC
    CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC
    TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG
    CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT
    GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG
    ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA
    GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA
    CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA
    CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC
    ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG
    CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT
    CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG
    AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG
    GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT
    CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT
    GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT
    GGGCGTCCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228349.1
    CDS339..4670
    Translation

    Target ORF information:

    RefSeq Version XM_005228292.4
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228292.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC
    TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG
    CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA
    GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC
    TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA
    TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG
    GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG
    TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC
    CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC
    CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC
    TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG
    CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT
    GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG
    ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA
    GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA
    CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA
    CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC
    ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG
    CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT
    CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG
    AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG
    GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT
    CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT
    GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT
    GGGCGTCCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35685
    Clone ID Related Accession (Same CDS sequence) XM_005228292.4 , XM_005228292.3
    Accession Version XM_005228292.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 14 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104743.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228292.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC
    TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG
    CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA
    GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC
    TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA
    TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG
    GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG
    TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC
    CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC
    CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC
    TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG
    CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT
    GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG
    ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA
    GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA
    CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA
    CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC
    ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG
    CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT
    CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG
    AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG
    GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT
    CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT
    GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT
    GGGCGTCCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228349.1
    CDS329..4660
    Translation

    Target ORF information:

    RefSeq Version XM_005228292.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228292.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC
    TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG
    CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA
    GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC
    TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA
    TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG
    GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG
    TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC
    CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC
    CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC
    TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG
    CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT
    GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG
    ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA
    GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA
    CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA
    CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC
    ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG
    CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT
    CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG
    AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG
    GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT
    CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT
    GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT
    GGGCGTCCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35686
    Clone ID Related Accession (Same CDS sequence) XM_005228293.4 , XM_005228293.3
    Accession Version XM_005228293.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 14 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228293.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT
    AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA
    CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT
    TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG
    TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA
    GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG
    ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA
    GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG
    CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG
    CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA
    CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG
    GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA
    CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA
    CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA
    ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA
    GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT
    GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA
    CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT
    CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA
    ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC
    TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC
    CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT
    ACACTGCCCC CTGGTGGGCG TCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228350.1
    CDS311..4537
    Translation

    Target ORF information:

    RefSeq Version XM_005228293.4
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228293.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT
    AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA
    CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT
    TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG
    TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA
    GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG
    ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA
    GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG
    CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG
    CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA
    CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG
    GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA
    CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA
    CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA
    ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA
    GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT
    GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA
    CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT
    CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA
    ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC
    TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC
    CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT
    ACACTGCCCC CTGGTGGGCG TCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35686
    Clone ID Related Accession (Same CDS sequence) XM_005228293.4 , XM_005228293.3
    Accession Version XM_005228293.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mediator of RNA polymerase II transcription subunit 14 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104743.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT
    AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA
    CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT
    TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG
    TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA
    GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG
    ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA
    GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG
    CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG
    CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA
    CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG
    GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA
    CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA
    CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA
    ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA
    GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT
    GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA
    CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT
    CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA
    ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC
    TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC
    CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT
    ACACTGCCCC CTGGTGGGCG TCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228350.1
    CDS329..4555
    Translation

    Target ORF information:

    RefSeq Version XM_005228293.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228293.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
    GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG
    GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC
    TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA
    ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA
    GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA
    GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC
    GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT
    ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA
    ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG
    CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG
    AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT
    GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG
    CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC
    TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA
    CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC
    CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA
    CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT
    GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT
    TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT
    GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC
    GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC
    TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG
    TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG
    AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA
    CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG
    TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT
    ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA
    CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT
    CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA
    AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA
    GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT
    CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC
    AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT
    CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC
    CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA
    GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC
    CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG
    GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA
    TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT
    TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC
    CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA
    GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA
    CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC
    AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG
    TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT
    TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT
    CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC
    CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT
    AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA
    CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT
    TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG
    TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA
    GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG
    ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA
    GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG
    CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG
    CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA
    CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG
    GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA
    CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA
    CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA
    ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA
    GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT
    GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA
    CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT
    CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA
    ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC
    TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC
    CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT
    ACACTGCCCC CTGGTGGGCG TCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.