MED14 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED14
Entrez Gene ID	529109
Full Name	mediator complex subunit 14
General protein information	Preferred Names mediator complex subunit 14 Names mediator of RNA polymerase II transcription subunit 14
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005228293.4	XP_005228350.1	mediator of RNA polymerase II transcription subunit 14 isoform X2
NM_001207031.1	NP_001193960.1	mediator of RNA polymerase II transcription subunit 14
XM_005228292.4	XP_005228349.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_024988080.1	XP_024843848.1	mediator of RNA polymerase II transcription subunit 14 isoform X3
XM_005228293.3	XP_005228350.1	mediator of RNA polymerase II transcription subunit 14 isoform X2
XM_005228292.3	XP_005228349.1	mediator of RNA polymerase II transcription subunit 14 isoform X1

Pathways from BioSystems

Homologies

	MED14	XP_001085867.1
Gallus gallus (chicken)	MED14	XP_416776.2
Xenopus tropicalis (tropical clawed frog)	med14	XP_002932839.1
Homo sapiens (human)	MED14	NP_004220.2
Bos taurus (cattle)	MED14	NP_001193960.1
Mus musculus (house mouse)	Med14	NP_001041673.1
Rattus norvegicus (Norway rat)	Med14	NP_001178656.1
Danio rerio (zebrafish)	med14	NP_997930.2
Anopheles gambiae (African malaria mosquito)	MED14_ANOGA	XP_315707.4
Drosophila melanogaster (fruit fly)	MED14	NP_612041.2
Pan troglodytes (chimpanzee)	MED14	XP_003317475.2
Canis lupus familiaris (dog)	MED14	XP_538001.2

Gene Ontology

The following MED14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238416	XM_024988080.1 Latest version!	Bos taurus mediator complex subunit 14 (MED14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa38551	NM_001207031.1 Latest version!	Bos taurus mediator complex subunit 14 (MED14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa35685	XM_005228292.4 Latest version!	Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa35686	XM_005228293.4 Latest version!	Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OBa35686	XM_005228293.3	Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa35685	XM_005228292.3	Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED14 ( XM_024988080.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843848.1 Bos taurus mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa238416
Clone ID Related Accession (Same CDS sequence)	XM_024988080.1
Accession Version	XM_024988080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC 
TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG 
CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA 
GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAACCCTGGA CCCTAGTTCT 
CCATACACTA TGGTGTCACC AAGTGGCCGA GCAGGGAACT GGCCAGGGTC TCCTCAAGTG 
TCTGGCCCCT CACCAGCAAC ACGCATGCCT GGGATGTCAC CAGCCAACCC ATCACTACAT 
TCTCCAGTTC CAGATGCTTC TCATTCCCCT CGAGCTGGAA CAAGTTCCCA AACGATGCCA 
ACAAACATGC CTCCACCTCG TAAACTACCT CAGCGCTCCT GGGCAGCCTC CATCCCTACC 
ATCCTCACTC ACAGTGCCTT GAACATTTTG CTGCTGCCCT CTCCAACGCC AGGCCTCGTG 
CCCGGCCTGG CAGGTAGTTA CCTATGTTCT CCACTTGAGA GATTCCTTGG ATCAGTCATC 
ATGAGACGCC ACCTTCAAAG AATTATTCAG CAGGAGACGC TGCAGCTGAT AAATTCCAAT 
GAACCCGGGG TGATCATGTT TAAGACTGAT GCACTGAAAT GCAGAGTAGC CCTTAGTCCT 
AAAACCAACC AAACACTTCA GCTAAAAGTG ACACCCGAAA ATGCAGGACA GTGGAAACCT 
GATGAACTTC AAGTTTTGGA GAAATTCTTT GAAACAAGAG TTGCAGGACC ACCATTTAAA 
GCCAATACAT TAATAGCTTT CACCAAGCTG TTAGGAGCTC CTACACACAT CCTCAGGGAC 
TGTGTGCATA TTATGAAGCT AGAGCTGTTT CCTGACCAGG CAACACAGCT AAAATGGAAT 
GTTCAGTTTT GCCTGACGAT CCCTCCCAGT GCACCACCAA TTGCACCTCC TGGGACACCT 
GCTGTGGTGC TGAAATCCAA AATGCTATTT TTTCTTCAGC TGACTCAGAA GACATCAGTC 
CCTCCCCAAG AACCTGTTAG TATTATAGTT CCAATCATTT ATGACATGGC TTCAGGTACC 
ACCCAGCAGG CAGACATTCC CAGGCAGCAG AACTCTTCTG TTGCTGCTCC CATGATGGTC 
AGCAACATTC TGAAGAGGTT TGCAGAGATG AACCCACCAC GACAAGGTGA ATGCACAATA 
TTTGCAGCTG TTCGTGATTT AATGGCTAAT CTTACACTGC CCCCTGGTGG GCGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843848.1
CDS	338..4537
Translation	MAPVQLENHQLVPPGGSGSGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDASKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGVNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTITSETLQLSNYSTHPIGNLSKNKLFVRLTRLPQYYIVVEMLEVPNKPTQLSYKYYFLSVNAVDRDDSPLMALLLQQFKENLQESVYRTKNGKQPRTSTKRKLSDDLCPVEPKKTKRSGEMRAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGINEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPSHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRFYRCLHFFVKRRGGPWRTQDSQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_024988080.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988080.1

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC 
TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG 
CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA 
GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAACCCTGGA CCCTAGTTCT 
CCATACACTA TGGTGTCACC AAGTGGCCGA GCAGGGAACT GGCCAGGGTC TCCTCAAGTG 
TCTGGCCCCT CACCAGCAAC ACGCATGCCT GGGATGTCAC CAGCCAACCC ATCACTACAT 
TCTCCAGTTC CAGATGCTTC TCATTCCCCT CGAGCTGGAA CAAGTTCCCA AACGATGCCA 
ACAAACATGC CTCCACCTCG TAAACTACCT CAGCGCTCCT GGGCAGCCTC CATCCCTACC 
ATCCTCACTC ACAGTGCCTT GAACATTTTG CTGCTGCCCT CTCCAACGCC AGGCCTCGTG 
CCCGGCCTGG CAGGTAGTTA CCTATGTTCT CCACTTGAGA GATTCCTTGG ATCAGTCATC 
ATGAGACGCC ACCTTCAAAG AATTATTCAG CAGGAGACGC TGCAGCTGAT AAATTCCAAT 
GAACCCGGGG TGATCATGTT TAAGACTGAT GCACTGAAAT GCAGAGTAGC CCTTAGTCCT 
AAAACCAACC AAACACTTCA GCTAAAAGTG ACACCCGAAA ATGCAGGACA GTGGAAACCT 
GATGAACTTC AAGTTTTGGA GAAATTCTTT GAAACAAGAG TTGCAGGACC ACCATTTAAA 
GCCAATACAT TAATAGCTTT CACCAAGCTG TTAGGAGCTC CTACACACAT CCTCAGGGAC 
TGTGTGCATA TTATGAAGCT AGAGCTGTTT CCTGACCAGG CAACACAGCT AAAATGGAAT 
GTTCAGTTTT GCCTGACGAT CCCTCCCAGT GCACCACCAA TTGCACCTCC TGGGACACCT 
GCTGTGGTGC TGAAATCCAA AATGCTATTT TTTCTTCAGC TGACTCAGAA GACATCAGTC 
CCTCCCCAAG AACCTGTTAG TATTATAGTT CCAATCATTT ATGACATGGC TTCAGGTACC 
ACCCAGCAGG CAGACATTCC CAGGCAGCAG AACTCTTCTG TTGCTGCTCC CATGATGGTC 
AGCAACATTC TGAAGAGGTT TGCAGAGATG AACCCACCAC GACAAGGTGA ATGCACAATA 
TTTGCAGCTG TTCGTGATTT AATGGCTAAT CTTACACTGC CCCCTGGTGG GCGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( NM_001207031.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193960.1 Bos taurus mediator complex subunit 14 (MED14), mRNA.

Price & Availability↑

CloneID	OBa38551
Clone ID Related Accession (Same CDS sequence)	NM_001207031.1
Accession Version	NM_001207031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-02
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 14
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000030.1. On or before May 19, 2011 this sequence version replaced XR_084733.1, XR_083551.1. ##Evidence-Data-START## Transcript exon combination :: SRR5721695.5559.1, SRR5721696.3157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA 
CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG 
TCTCCGGGAA ATCTGCATGC TGCCAGCTCC CCGAGTGGGG CTTTGAGAGC CCCATCACCA 
GCATCATTTG TTCCAACTCC TCCCCCATCC TCGCATGGAA TCTCAATAGG ACCAGGGGCC 
AGTTTTGCTA GTCCACATGG AACCCTGGAC CCTAGTTCTC CATACACTAT GGTGTCACCA 
AGTGGCCGAG CAGGGAACTG GCCAGGGTCT CCTCAAGTGT CTGGCCCCTC ACCAGCAACA 
CGCATGCCTG GGATGTCACC AGCCAACCCA TCACTACATT CTCCAGTTCC AGATGCTTCT 
CATTCCCCTC GAGCTGGAAC AAGTTCCCAA ACGATGCCAA CAAACATGCC TCCACCTCGT 
AAACTACCTC AGCGCTCCTG GGCAGCCTCC ATCCCTACCA TCCTCACTCA CAGTGCCTTG 
AACATTTTGC TGCTGCCCTC TCCAACGCCA GGCCTCGTGC CCGGCCTGGC AGGTAGTTAC 
CTATGTTCTC CACTTGAGAG ATTCCTTGGA TCAGTCATCA TGAGACGCCA CCTTCAAAGA 
ATTATTCAGC AGGAGACGCT GCAGCTGATA AATTCCAATG AACCCGGGGT GATCATGTTT 
AAGACTGATG CACTGAAATG CAGAGTAGCC CTTAGTCCTA AAACCAACCA AACACTTCAG 
CTAAAAGTGA CACCCGAAAA TGCAGGACAG TGGAAACCTG ATGAACTTCA AGTTTTGGAG 
AAATTCTTTG AAACAAGAGT TGCAGGACCA CCATTTAAAG CCAATACATT AATAGCTTTC 
ACCAAGCTGT TAGGAGCTCC TACACACATC CTCAGGGACT GTGTGCATAT TATGAAGCTA 
GAGCTGTTTC CTGACCAGGC AACACAGCTA AAATGGAATG TTCAGTTTTG CCTGACGATC 
CCTCCCAGTG CACCACCAAT TGCACCTCCT GGGACACCTG CTGTGGTGCT GAAATCCAAA 
ATGCTATTTT TTCTTCAGCT GACTCAGAAG ACATCAGTCC CTCCCCAAGA ACCTGTTAGT 
ATTATAGTTC CAATCATTTA TGACATGGCT TCAGGTACCA CCCAGCAGGC AGACATTCCC 
AGGCAGCAGA ACTCTTCTGT TGCTGCTCCC ATGATGGTCA GCAACATTCT GAAGAGGTTT 
GCAGAGATGA ACCCACCACG ACAAGGTGAA TGCACAATAT TTGCAGCTGT TCGTGATTTA 
ATGGCTAATC TTACACTGCC CCCTGGTGGG CGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193960.1
CDS	109..4467
Translation	MAPVQLENHQLVPPGGSGSGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDASKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGVNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTITSETLQLSNYSTHPIGNLSKNKLFVRLTRLPQYYIVVEMLEVPNKPTQLSYKYYFLSVNAVDRDDSPLMALLLQQFKENLQESVYRTKNGKQPRTSTKRKLSDDLCPVEPKKTKRSGEMRAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGINEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPSHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	NM_001207031.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 14 (MED14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001207031.1

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA 
CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG 
TCTCCGGGAA ATCTGCATGC TGCCAGCTCC CCGAGTGGGG CTTTGAGAGC CCCATCACCA 
GCATCATTTG TTCCAACTCC TCCCCCATCC TCGCATGGAA TCTCAATAGG ACCAGGGGCC 
AGTTTTGCTA GTCCACATGG AACCCTGGAC CCTAGTTCTC CATACACTAT GGTGTCACCA 
AGTGGCCGAG CAGGGAACTG GCCAGGGTCT CCTCAAGTGT CTGGCCCCTC ACCAGCAACA 
CGCATGCCTG GGATGTCACC AGCCAACCCA TCACTACATT CTCCAGTTCC AGATGCTTCT 
CATTCCCCTC GAGCTGGAAC AAGTTCCCAA ACGATGCCAA CAAACATGCC TCCACCTCGT 
AAACTACCTC AGCGCTCCTG GGCAGCCTCC ATCCCTACCA TCCTCACTCA CAGTGCCTTG 
AACATTTTGC TGCTGCCCTC TCCAACGCCA GGCCTCGTGC CCGGCCTGGC AGGTAGTTAC 
CTATGTTCTC CACTTGAGAG ATTCCTTGGA TCAGTCATCA TGAGACGCCA CCTTCAAAGA 
ATTATTCAGC AGGAGACGCT GCAGCTGATA AATTCCAATG AACCCGGGGT GATCATGTTT 
AAGACTGATG CACTGAAATG CAGAGTAGCC CTTAGTCCTA AAACCAACCA AACACTTCAG 
CTAAAAGTGA CACCCGAAAA TGCAGGACAG TGGAAACCTG ATGAACTTCA AGTTTTGGAG 
AAATTCTTTG AAACAAGAGT TGCAGGACCA CCATTTAAAG CCAATACATT AATAGCTTTC 
ACCAAGCTGT TAGGAGCTCC TACACACATC CTCAGGGACT GTGTGCATAT TATGAAGCTA 
GAGCTGTTTC CTGACCAGGC AACACAGCTA AAATGGAATG TTCAGTTTTG CCTGACGATC 
CCTCCCAGTG CACCACCAAT TGCACCTCCT GGGACACCTG CTGTGGTGCT GAAATCCAAA 
ATGCTATTTT TTCTTCAGCT GACTCAGAAG ACATCAGTCC CTCCCCAAGA ACCTGTTAGT 
ATTATAGTTC CAATCATTTA TGACATGGCT TCAGGTACCA CCCAGCAGGC AGACATTCCC 
AGGCAGCAGA ACTCTTCTGT TGCTGCTCCC ATGATGGTCA GCAACATTCT GAAGAGGTTT 
GCAGAGATGA ACCCACCACG ACAAGGTGAA TGCACAATAT TTGCAGCTGT TCGTGATTTA 
ATGGCTAATC TTACACTGCC CCCTGGTGGG CGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_005228292.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228349.1 Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

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CloneID	OBa35685
Clone ID Related Accession (Same CDS sequence)	XM_005228292.4 , XM_005228292.3
Accession Version	XM_005228292.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228292.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC 
TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG 
CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA 
GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC 
TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA 
TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG 
GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG 
TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC 
CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC 
CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC 
TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG 
CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT 
GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG 
ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA 
GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA 
CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA 
CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC 
ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG 
CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT 
CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG 
AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG 
GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT 
CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT 
GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT 
GGGCGTCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228349.1
CDS	339..4670
Translation	MAPVQLENHQLVPPGGSGSGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDASKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGVNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTITSETLQLSNYSTHPIGNLSKNKLFVRLTRLPQYYIVVEMLEVPNKPTQLSYKYYFLSVNAVDRDDSPLMALLLQQFKENLQESVYRTKNGKQPRTSTKRKLSDDLCPVEPKKTKRSGEMRAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGINEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPSHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRFYRCLHFFVKRRGGPWRTQDSQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_005228292.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228292.4

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC 
TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG 
CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA 
GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC 
TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA 
TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG 
GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG 
TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC 
CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC 
CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC 
TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG 
CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT 
GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG 
ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA 
GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA 
CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA 
CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC 
ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG 
CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT 
CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG 
AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG 
GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT 
CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT 
GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT 
GGGCGTCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_005228292.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228349.1 Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

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CloneID	OBa35685
Clone ID Related Accession (Same CDS sequence)	XM_005228292.4 , XM_005228292.3
Accession Version	XM_005228292.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104743.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228292.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC 
TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG 
CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA 
GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC 
TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA 
TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG 
GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG 
TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC 
CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC 
CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC 
TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG 
CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT 
GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG 
ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA 
GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA 
CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA 
CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC 
ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG 
CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT 
CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG 
AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG 
GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT 
CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT 
GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT 
GGGCGTCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228349.1
CDS	329..4660
Translation	MAPVQLENHQLVPPGGSGSGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDASKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGVNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTITSETLQLSNYSTHPIGNLSKNKLFVRLTRLPQYYIVVEMLEVPNKPTQLSYKYYFLSVNAVDRDDSPLMALLLQQFKENLQESVYRTKNGKQPRTSTKRKLSDDLCPVEPKKTKRSGEMRAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGINEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPSHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRFYRCLHFFVKRRGGPWRTQDSQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_005228292.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228292.3

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTTCTACAG GTGTCTGCAC 
TTCTTTGTTA AAAGGCGAGG AGGTCCTTGG AGGACACAAG ACAGCCAGCC ATTTCCAAAG 
CAGCCAGGAT CATCAGGTGC TTATCCTCTT ACTTCACCCC CTACATCTTA CCACAGCACA 
GTCAACCAGT CTCCCTCTAT GATGCATACA CAGTCTCCGG GAAATCTGCA TGCTGCCAGC 
TCCCCGAGTG GGGCTTTGAG AGCCCCATCA CCAGCATCAT TTGTTCCAAC TCCTCCCCCA 
TCCTCGCATG GAATCTCAAT AGGACCAGGG GCCAGTTTTG CTAGTCCACA TGGAACCCTG 
GACCCTAGTT CTCCATACAC TATGGTGTCA CCAAGTGGCC GAGCAGGGAA CTGGCCAGGG 
TCTCCTCAAG TGTCTGGCCC CTCACCAGCA ACACGCATGC CTGGGATGTC ACCAGCCAAC 
CCATCACTAC ATTCTCCAGT TCCAGATGCT TCTCATTCCC CTCGAGCTGG AACAAGTTCC 
CAAACGATGC CAACAAACAT GCCTCCACCT CGTAAACTAC CTCAGCGCTC CTGGGCAGCC 
TCCATCCCTA CCATCCTCAC TCACAGTGCC TTGAACATTT TGCTGCTGCC CTCTCCAACG 
CCAGGCCTCG TGCCCGGCCT GGCAGGTAGT TACCTATGTT CTCCACTTGA GAGATTCCTT 
GGATCAGTCA TCATGAGACG CCACCTTCAA AGAATTATTC AGCAGGAGAC GCTGCAGCTG 
ATAAATTCCA ATGAACCCGG GGTGATCATG TTTAAGACTG ATGCACTGAA ATGCAGAGTA 
GCCCTTAGTC CTAAAACCAA CCAAACACTT CAGCTAAAAG TGACACCCGA AAATGCAGGA 
CAGTGGAAAC CTGATGAACT TCAAGTTTTG GAGAAATTCT TTGAAACAAG AGTTGCAGGA 
CCACCATTTA AAGCCAATAC ATTAATAGCT TTCACCAAGC TGTTAGGAGC TCCTACACAC 
ATCCTCAGGG ACTGTGTGCA TATTATGAAG CTAGAGCTGT TTCCTGACCA GGCAACACAG 
CTAAAATGGA ATGTTCAGTT TTGCCTGACG ATCCCTCCCA GTGCACCACC AATTGCACCT 
CCTGGGACAC CTGCTGTGGT GCTGAAATCC AAAATGCTAT TTTTTCTTCA GCTGACTCAG 
AAGACATCAG TCCCTCCCCA AGAACCTGTT AGTATTATAG TTCCAATCAT TTATGACATG 
GCTTCAGGTA CCACCCAGCA GGCAGACATT CCCAGGCAGC AGAACTCTTC TGTTGCTGCT 
CCCATGATGG TCAGCAACAT TCTGAAGAGG TTTGCAGAGA TGAACCCACC ACGACAAGGT 
GAATGCACAA TATTTGCAGC TGTTCGTGAT TTAATGGCTA ATCTTACACT GCCCCCTGGT 
GGGCGTCCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_005228293.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228350.1 Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35686
Clone ID Related Accession (Same CDS sequence)	XM_005228293.4 , XM_005228293.3
Accession Version	XM_005228293.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228293.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA 
CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG 
TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA 
GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG 
ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA 
GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG 
CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG 
CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA 
CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG 
GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA 
CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA 
CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA 
ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA 
GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT 
GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA 
CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT 
CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA 
ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC 
TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC 
CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT 
ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228350.1
CDS	311..4537
Translation	MAPVQLENHQLVPPGGSGSGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDASKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGVNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTITSETLQLSNYSTHPIGNLSKNKLFVRLTRLPQYYIVVEMLEVPNKPTQLSYKYYFLSVNAVDRDDSPLMALLLQQFKENLQESVYRTKNGKQPRTSTKRKLSDDLCPVEPKKTKRSGEMRAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGINEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPSHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_005228293.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228293.4

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA 
CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG 
TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA 
GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG 
ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA 
GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG 
CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG 
CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA 
CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG 
GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA 
CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA 
CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA 
ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA 
GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT 
GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA 
CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT 
CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA 
ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC 
TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC 
CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT 
ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_005228293.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228350.1 Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35686
Clone ID Related Accession (Same CDS sequence)	XM_005228293.4 , XM_005228293.3
Accession Version	XM_005228293.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104743.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA 
CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG 
TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA 
GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG 
ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA 
GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG 
CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG 
CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA 
CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG 
GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA 
CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA 
CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA 
ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA 
GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT 
GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA 
CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT 
CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA 
ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC 
TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC 
CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT 
ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228350.1
CDS	329..4555
Translation	MAPVQLENHQLVPPGGSGSGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDASKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGVNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTITSETLQLSNYSTHPIGNLSKNKLFVRLTRLPQYYIVVEMLEVPNKPTQLSYKYYFLSVNAVDRDDSPLMALLLQQFKENLQESVYRTKNGKQPRTSTKRKLSDDLCPVEPKKTKRSGEMRAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGINEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPSHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_005228293.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228293.3

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCAG CGGCAGCGGC 
GGCCCCGCGT CAGCCCCGGC GCCGCCTCCT CCCGGAGCCG CCGTGGCAGC GGCCGCTGCG 
GCCGCGGCTA GCCCTGGCTA CCGGCTGAGC ACCCTCATCG AATTTCTGCT GCACCGGGCC 
TACTCGGAGC TCATGGTGCT GACGGACCTG CTGCCAAGGA AATCTGATGT GGAAAGGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGCCAACTCT TTGTTCGACT TTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAAGTG GAAAAATGTG CGATGATCTC AAGCTTCTTA 
GATCAGCAAG CCATCCTGTT TGTGGACACT GCGGATCGCC TGGCCTCGTT AGCTAGAGAC 
GCTCTGGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCAT ATGCTATTGA TGTGCTGACT 
ACTGGGTCTT ATCCACGGCT GCCAACCTGC ATCAGAGATA AAATTATTCC TCCAGACCCA 
ATTACCAAAA TTGAGAAGCA AGCTACACTT CACCAGCTGA ACCAGATTCT TAGACATCGG 
CTTGTAACCA CGGATCTTCC TCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGAGTG 
AAGTTTCGAG TTGAAGGAGA ATTTGAAGCC ACCTTGACTG TGATGGGAGA TGACCCTGAT 
GTTCCGTGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ATAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAGATCAAC TTTATCCATC AACTGGTACA ATCTAGGCTC 
TTTGCTGATG AGAAACCTCT TCAGGACATG TACAACTGCC TGCATTCTTT CTGCTTATCA 
CTTCAGTTAG AAGTGCTACA TTCTCAAACT CTAATGTTAA TCCGAGAGCG GTGGGGAGAC 
CTTGTACAGG TGGAAAGGTA TCATGCAGGA AAATGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACAGCA TCTGTTCACA AAGTTACAAT TAAAATTGAT 
GAGAATGATG CCTCCAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC GGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GATCATTTAT CAATAGAAAA ACTCCTGATT 
GACAGTGTCC ATGCACGAGC TCATCAGAAG CTTCAGGAAC TGAAGGCCAT TCTTAGAGGC 
GTCAATGCTA ATGAAAACTC TTCCATAGAG ACTGCACTTC CAGCTCTTGT TGTTCCCATC 
TTGGAGCCCT GTGGTAATTC AGAGTGTCTA CACATTTTTG TCGATTTGCA TTCTGGAATG 
TTCCAGTTGA TGCTTTATGG ACTTGACCAA GCCACACTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGGAT CATTCCTTGG ATTCAACAAC TTAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACACCTG CCTACAATAA CCAGTGAAAC ATTGCAGCTG 
TCCAATTATT CAACTCATCC CATAGGAAAC CTGTCTAAGA ATAAGCTGTT TGTAAGACTT 
ACCCGTCTTC CACAATACTA CATTGTTGTA GAGATGTTAG AAGTTCCCAA TAAACCCACA 
CAACTGTCGT ACAAGTACTA CTTCTTGTCT GTGAATGCAG TGGATCGTGA CGACAGCCCT 
CTCATGGCCC TCTTGCTGCA GCAGTTTAAG GAAAACCTCC AGGAATCAGT TTATCGTACA 
AAAAATGGGA AACAGCCTAG AACCAGTACC AAGCGCAAGT TGTCTGATGA TTTGTGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGCGTG CCTTCAATAA AGTTCTAGCT 
CACTTCGTTG CTATGTGTGA TACAAACATG CCATTTGTAG GACTTCGGTT GGAGTTGTCC 
AATCTGGAGA TTCCACATCA AGGAGTGCAA ATGGAAGGTG ATGGCTTCAG CCACGCGATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT ATAAATGAGG AAACCCAAAA GGCTCTGGAC 
CGCTCTCTCC TTGACTGCAC TTTCCGATTA CAAGGTAGAA ATAACCGCAC GTGGGTGGCA 
GAGCTAGTGT TTGCAAATTG TCCCCTTAAT GGCACTTCTA CCAGGGAACA AGGACCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGAAAAGTG 
GTTGAAATGT TCCTTAATGA TTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCCAGT CATCTAAACA TTTTCTCAGA AGTTCGTGTT 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGTTC AATTAGTATC 
CAATGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTGGG TCCAAACTCA 
GGATGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACA 
CCAAATGTGG TTCAGTTATT ACAGGTATTG TTTGATACTC AGGCACCGTT GAATGCCATC 
AACAAACTGC CCACTGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCCTACCAG 
TGCTTCTCCA TCCTGCCACA GTCGTCCACC CACATCCGAC TGGCCTTCAG GAACATGTAT 
TGCATTGATA TATACTGCCG AAGTCGAGGT GTTGTGGCAA TACGGGACGG GGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTTGAAGGT TTTTATCCTG CACCAGGACT CAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATCTCACA ACTCCAACCA 
CCGCCACAGC AACAGCCATT TCCAAAGCAG CCAGGATCAT CAGGTGCTTA TCCTCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTC AACCAGTCTC CCTCTATGAT GCATACACAG 
TCTCCGGGAA CCCTGGACCC TAGTTCTCCA TACACTATGG TGTCACCAAG TGGCCGAGCA 
GGGAACTGGC CAGGGTCTCC TCAAGTGTCT GGCCCCTCAC CAGCAACACG CATGCCTGGG 
ATGTCACCAG CCAACCCATC ACTACATTCT CCAGTTCCAG ATGCTTCTCA TTCCCCTCGA 
GCTGGAACAA GTTCCCAAAC GATGCCAACA AACATGCCTC CACCTCGTAA ACTACCTCAG 
CGCTCCTGGG CAGCCTCCAT CCCTACCATC CTCACTCACA GTGCCTTGAA CATTTTGCTG 
CTGCCCTCTC CAACGCCAGG CCTCGTGCCC GGCCTGGCAG GTAGTTACCT ATGTTCTCCA 
CTTGAGAGAT TCCTTGGATC AGTCATCATG AGACGCCACC TTCAAAGAAT TATTCAGCAG 
GAGACGCTGC AGCTGATAAA TTCCAATGAA CCCGGGGTGA TCATGTTTAA GACTGATGCA 
CTGAAATGCA GAGTAGCCCT TAGTCCTAAA ACCAACCAAA CACTTCAGCT AAAAGTGACA 
CCCGAAAATG CAGGACAGTG GAAACCTGAT GAACTTCAAG TTTTGGAGAA ATTCTTTGAA 
ACAAGAGTTG CAGGACCACC ATTTAAAGCC AATACATTAA TAGCTTTCAC CAAGCTGTTA 
GGAGCTCCTA CACACATCCT CAGGGACTGT GTGCATATTA TGAAGCTAGA GCTGTTTCCT 
GACCAGGCAA CACAGCTAAA ATGGAATGTT CAGTTTTGCC TGACGATCCC TCCCAGTGCA 
CCACCAATTG CACCTCCTGG GACACCTGCT GTGGTGCTGA AATCCAAAAT GCTATTTTTT 
CTTCAGCTGA CTCAGAAGAC ATCAGTCCCT CCCCAAGAAC CTGTTAGTAT TATAGTTCCA 
ATCATTTATG ACATGGCTTC AGGTACCACC CAGCAGGCAG ACATTCCCAG GCAGCAGAAC 
TCTTCTGTTG CTGCTCCCAT GATGGTCAGC AACATTCTGA AGAGGTTTGC AGAGATGAAC 
CCACCACGAC AAGGTGAATG CACAATATTT GCAGCTGTTC GTGATTTAAT GGCTAATCTT 
ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

MED14 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報