S100A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	S100A1
Entrez Gene ID	528735
Full Name	S100 calcium binding protein A1
General protein information	Preferred Names S100 calcium binding protein A1 Names protein S100-A1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005203720.3	XP_005203777.1	protein S100-A1 isoform X2
XM_005203719.2	XP_005203776.1	protein S100-A1 isoform X1
XM_010802915.3	XP_010801217.1	protein S100-A1 isoform X3
XM_005203721.4	XP_005203778.1	protein S100-A1 isoform X3
XM_010802915.2	XP_010801217.1	protein S100-A1 isoform X3
XM_005203721.3	XP_005203778.1	protein S100-A1 isoform X3
NM_001099042.2	NP_001092512.1	protein S100-A1

Pathways from BioSystems

Homologies

Homo sapiens (human)	S100A1	NP_006262.1
	S100A1	XP_001111052.1
Canis lupus familiaris (dog)	S100A1	XP_005622873.1
Mus musculus (house mouse)	S100a1	NP_035439.1
Danio rerio (zebrafish)	s100a1	NP_001082820.1
Rattus norvegicus (Norway rat)	S100a1	NP_001007637.1
Gallus gallus (chicken)	S100A1	XP_003642677.1
Bos taurus (cattle)	S100A1	NP_001092512.1
Xenopus tropicalis (tropical clawed frog)	LOC101734724	XP_004920660.1
Pan troglodytes (chimpanzee)	S100A1	XP_513820.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Structural and motional changes induced in apo-S100A1 protein by the disulfide formation between its Cys 85 residue and beta-mercaptoethanol.
Zhukov I, Ejchart A, Bierzy?ski A
Biochemistry47(2)640-50(2008 Jan)

Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation.
Goch G, Vdovenko S, Koz?owska H, Bierzy?ski A
The FEBS journal272(10)2557-65(2005 May)

S100A1 is a novel molecular chaperone and a member of the Hsp70/Hsp90 multichaperone complex.
Okada M, Hatakeyama T, Itoh H, Tokuta N, Tokumitsu H, Kobayashi R
The Journal of biological chemistry279(6)4221-33(2004 Feb)

CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family.
Filipek A, Jastrzebska B, Nowotny M, Kuznicki J
The Journal of biological chemistry277(32)28848-52(2002 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

beta-mercaptoethanol modification of apo-S100A1 makes its structure more similar to that of holo-S100A1, so that it becomes much better adjusted for calcium coordination.
Title: Structural and motional changes induced in apo-S100A1 protein by the disulfide formation between its Cys 85 residue and beta-mercaptoethanol.

Results suggest that S100A1 can act as a linker between the calcium and redox signalling pathways.
Title: Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation.

identified S100A1, but not calmodulin or other S100 proteins, as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex (S100A1)
Title: S100A1 is a novel molecular chaperone and a member of the Hsp70/Hsp90 multichaperone complex.

The following S100A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S100A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa03301	NM_001099042.2 Latest version!	Bos taurus S100 calcium binding protein A1 (S100A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa03301	XM_005203721.4 Latest version!	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa03301	XM_010802915.3 Latest version!	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa17316	XM_005203719.2 Latest version!	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa17317	XM_005203720.3 Latest version!	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OBa03301	XM_010802915.2	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa03301	XM_005203721.3	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S100A1 ( NM_001099042.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001092512.1 Bos taurus S100 calcium binding protein A1 (S100A1), mRNA.

Price & Availability↑

CloneID	OBa03301
Clone ID Related Accession (Same CDS sequence)	XM_010802915.3 , XM_005203721.4 , XM_010802915.2 , XM_005203721.3 , NM_001099042.2
Accession Version	NM_001099042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Bos taurus(cattle)
Product	protein S100-A1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC141991.1. On Aug 30, 2012 this sequence version replaced NM_001099042.1. ##Evidence-Data-START## Transcript exon combination :: EE244617.1, DV835417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092512.1
CDS	291..575
Translation	MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDADAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Target ORF information:

RefSeq Version	NM_001099042.2
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein A1 (S100A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099042.2

ORF Insert Sequence:

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100A1 ( XM_010802915.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801217.1 Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa03301
Clone ID Related Accession (Same CDS sequence)	XM_010802915.3 , XM_005203721.4 , XM_010802915.2 , XM_005203721.3 , NM_001099042.2
Accession Version	XM_010802915.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein S100-A1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802915.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801217.1
CDS	136..420
Translation	MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDADAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Target ORF information:

RefSeq Version	XM_010802915.3
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802915.3

ORF Insert Sequence:

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100A1 ( XM_005203721.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203778.1 Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa03301
Clone ID Related Accession (Same CDS sequence)	XM_010802915.3 , XM_005203721.4 , XM_010802915.2 , XM_005203721.3 , NM_001099042.2
Accession Version	XM_005203721.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein S100-A1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203721.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203778.1
CDS	166..450
Translation	MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDADAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Target ORF information:

RefSeq Version	XM_005203721.4
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203721.4

ORF Insert Sequence:

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100A1 ( XM_010802915.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801217.1 Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa03301
Clone ID Related Accession (Same CDS sequence)	XM_010802915.3 , XM_005203721.4 , XM_010802915.2 , XM_005203721.3 , NM_001099042.2
Accession Version	XM_010802915.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein S100-A1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801217.1
CDS	126..410
Translation	MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDADAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Target ORF information:

RefSeq Version	XM_010802915.2
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802915.2

ORF Insert Sequence:

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100A1 ( XM_005203721.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203778.1 Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa03301
Clone ID Related Accession (Same CDS sequence)	XM_010802915.3 , XM_005203721.4 , XM_010802915.2 , XM_005203721.3 , NM_001099042.2
Accession Version	XM_005203721.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein S100-A1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203778.1
CDS	164..448
Translation	MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDADAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Target ORF information:

RefSeq Version	XM_005203721.3
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203721.3

ORF Insert Sequence:

ATGGGCTCTG AGCTGGAGAC GGCGATGGAG ACTCTCATCA ATGTGTTCCA CGCCCACTCG 
GGCAAAGAGG GAGACAAGTA CAAGCTGAGC AAGAAGGAGC TGAAAGAGCT GCTGCAGACG 
GAGCTCTCCG GCTTCCTGGA TGCCCAGAAG GATGCAGACG CTGTGGACAA GGTGATGAAG 
GAGCTGGATG AGAACGGAGA TGGAGAGGTG GACTTCCAGG AGTATGTGGT GCTGGTGGCT 
GCCCTCACGG TGGCGTGCAA CAACTTCTTC TGGGAGAACA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100A1 ( XM_005203719.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203776.1 Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa17316
Clone ID Related Accession (Same CDS sequence)	XM_005203719.2
Accession Version	XM_005203719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein S100-A1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203719.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCCCC TCAGTCAGGT GCACACACCT CCTCTCTGCC CGGCTGCACA CCCATTATTA 
CTGGGAGGGG AGGGTGGAGC ATGGGAGGAA GAGTCCAGAA GGCCTCTCAG TGTTCCTTCT 
TCCCAGGACC TTTCCCACCT CCCTTCCTCC TGGGCCCTGC CATTTGGGGC CCCTGCGTGG 
GGGTGGCATC CAAGGCTGGA GAAGAGGACA CAGGGCAGCC TGTTTCAGCC CAGGAGACAG 
AGGGCGCCTC TGTCCACTCC TGGCCCCTCG CCCCACTGGG TGTCTGTCTG TGAAGGGGTG 
GAGCCGGTGC GGAGGGGGCG TCGCAGGGGA AGGACAGCTG AAGACAGGTC TTCCTGCACA 
GCTCCAGGAG CCACATTTGC AGCCTCGTCT GCCTGTCCAG AACCTGCTCC CAGGCTGACA 
GTAAGCTTCG AGATGGGCTC TGAGCTGGAG ACGGCGATGG AGACTCTCAT CAATGTGTTC 
CACGCCCACT CGGGCAAAGA GGGAGACAAG TACAAGCTGA GCAAGAAGGA GCTGAAAGAG 
CTGCTGCAGA CGGAGCTCTC CGGCTTCCTG GATGCCCAGA AGGATGCAGA CGCTGTGGAC 
AAGGTGATGA AGGAGCTGGA TGAGAACGGA GATGGAGAGG TGGACTTCCA GGAGTATGTG 
GTGCTGGTGG CTGCCCTCAC GGTGGCGTGC AACAACTTCT TCTGGGAGAA CAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203776.1
CDS	418..1134
Translation	MSPLSQVHTPPLCPAAHPLLLGGEGGAWEEESRRPLSVPSSQDLSHLPSSWALPFGAPAWGWHPRLEKRTQGSLFQPRRQRAPLSTPGPSPHWVSVCEGVEPVRRGRRRGRTAEDRSSCTAPGATFAASSACPEPAPRLTVSFEMGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDADAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Target ORF information:

RefSeq Version	XM_005203719.2
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203719.2

ORF Insert Sequence:

ATGAGCCCCC TCAGTCAGGT GCACACACCT CCTCTCTGCC CGGCTGCACA CCCATTATTA 
CTGGGAGGGG AGGGTGGAGC ATGGGAGGAA GAGTCCAGAA GGCCTCTCAG TGTTCCTTCT 
TCCCAGGACC TTTCCCACCT CCCTTCCTCC TGGGCCCTGC CATTTGGGGC CCCTGCGTGG 
GGGTGGCATC CAAGGCTGGA GAAGAGGACA CAGGGCAGCC TGTTTCAGCC CAGGAGACAG 
AGGGCGCCTC TGTCCACTCC TGGCCCCTCG CCCCACTGGG TGTCTGTCTG TGAAGGGGTG 
GAGCCGGTGC GGAGGGGGCG TCGCAGGGGA AGGACAGCTG AAGACAGGTC TTCCTGCACA 
GCTCCAGGAG CCACATTTGC AGCCTCGTCT GCCTGTCCAG AACCTGCTCC CAGGCTGACA 
GTAAGCTTCG AGATGGGCTC TGAGCTGGAG ACGGCGATGG AGACTCTCAT CAATGTGTTC 
CACGCCCACT CGGGCAAAGA GGGAGACAAG TACAAGCTGA GCAAGAAGGA GCTGAAAGAG 
CTGCTGCAGA CGGAGCTCTC CGGCTTCCTG GATGCCCAGA AGGATGCAGA CGCTGTGGAC 
AAGGTGATGA AGGAGCTGGA TGAGAACGGA GATGGAGAGG TGGACTTCCA GGAGTATGTG 
GTGCTGGTGG CTGCCCTCAC GGTGGCGTGC AACAACTTCT TCTGGGAGAA CAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100A1 ( XM_005203720.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203777.1 Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X2, mRNA.

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CloneID	OBa17317
Clone ID Related Accession (Same CDS sequence)	XM_005203720.3
Accession Version	XM_005203720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein S100-A1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCCCC TCAGTCAGGT GCACACACCT CCTCTCTGCC CGGCTGCACA CCCATTATTA 
CTGGGAGGGG AGGGTGGAGC ATGGGAGGAA GAGTCCAGAA GGCCTCTCAG TGTTCCTTCT 
TCCCAGGACC TTTCCCACCT CCCTTCCTCC TGGGCCCTGC CATTTGGGGC CCCTGCGTGG 
GGGTGGCATC CAAGGCTGGA GAAGAGGACA CAGGGCAGCC TGTTTCAGCC CAGGAGACAG 
AGGGCGCCTC TGTCCACTCC TGGCCCCTCG CCCCACTGGG TGTCTGTCTG TGAAGGGGTG 
GAGCCGGTGC GGAGGGGGCG TCGCAGGGGA AGGACAGCTG AAGACAGGTC TTCCTGCACA 
GCTCCAGGAG CCACATTTGC AGCCTCGTCT GCCTGTCCAG AACCTGCTCC CAGGCTGACA 
GCCCAGAAGG ATGCAGACGC TGTGGACAAG GTGATGAAGG AGCTGGATGA GAACGGAGAT 
GGAGAGGTGG ACTTCCAGGA GTATGTGGTG CTGGTGGCTG CCCTCACGGT GGCGTGCAAC 
AACTTCTTCT GGGAGAACAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203777.1
CDS	418..981
Translation	MSPLSQVHTPPLCPAAHPLLLGGEGGAWEEESRRPLSVPSSQDLSHLPSSWALPFGAPAWGWHPRLEKRTQGSLFQPRRQRAPLSTPGPSPHWVSVCEGVEPVRRGRRRGRTAEDRSSCTAPGATFAASSACPEPAPRLTAQKDADAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

Target ORF information:

RefSeq Version	XM_005203720.3
Organism	Bos taurus(cattle)
Definition	Bos taurus S100 calcium binding protein A1 (S100A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203720.3

ORF Insert Sequence:

ATGAGCCCCC TCAGTCAGGT GCACACACCT CCTCTCTGCC CGGCTGCACA CCCATTATTA 
CTGGGAGGGG AGGGTGGAGC ATGGGAGGAA GAGTCCAGAA GGCCTCTCAG TGTTCCTTCT 
TCCCAGGACC TTTCCCACCT CCCTTCCTCC TGGGCCCTGC CATTTGGGGC CCCTGCGTGG 
GGGTGGCATC CAAGGCTGGA GAAGAGGACA CAGGGCAGCC TGTTTCAGCC CAGGAGACAG 
AGGGCGCCTC TGTCCACTCC TGGCCCCTCG CCCCACTGGG TGTCTGTCTG TGAAGGGGTG 
GAGCCGGTGC GGAGGGGGCG TCGCAGGGGA AGGACAGCTG AAGACAGGTC TTCCTGCACA 
GCTCCAGGAG CCACATTTGC AGCCTCGTCT GCCTGTCCAG AACCTGCTCC CAGGCTGACA 
GCCCAGAAGG ATGCAGACGC TGTGGACAAG GTGATGAAGG AGCTGGATGA GAACGGAGAT 
GGAGAGGTGG ACTTCCAGGA GTATGTGGTG CTGGTGGCTG CCCTCACGGT GGCGTGCAAC 
AACTTCTTCT GGGAGAACAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Structural and motional changes induced in apo-S100A1 protein by the disulfide formation between its Cys 85 residue and beta-mercaptoethanol.
Biochemistry47(2)640-50(2008 Jan)
Zhukov I,Ejchart A,Bierzy?ski A

Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation.
The FEBS journal272(10)2557-65(2005 May)
Goch G,Vdovenko S,Koz?owska H,Bierzy?ski A

S100A1 is a novel molecular chaperone and a member of the Hsp70/Hsp90 multichaperone complex.
The Journal of biological chemistry279(6)4221-33(2004 Feb)
Okada M,Hatakeyama T,Itoh H,Tokuta N,Tokumitsu H,Kobayashi R

CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family.
The Journal of biological chemistry277(32)28848-52(2002 Aug)
Filipek A,Jastrzebska B,Nowotny M,Kuznicki J

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S100A1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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