NEURL4 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
NM_001192661.1	NP_001179590.1	neuralized-like protein 4
NM_001192661.3	NP_001179590.1	neuralized-like protein 4
NM_001192661.2	NP_001179590.1	neuralized-like protein 4
XM_015458630.1	XP_015314116.1	neuralized-like protein 4 isoform X3
XM_005220232.4	XP_005220289.1	neuralized-like protein 4 isoform X1
XM_015458630.2	XP_015314116.1	neuralized-like protein 4 isoform X3
XM_015458629.1	XP_015314115.1	neuralized-like protein 4 isoform X2
XM_015458631.1	XP_015314117.1	neuralized-like protein 4 isoform X4
XM_005220232.3	XP_005220289.1	neuralized-like protein 4 isoform X1
XM_010815925.2	XP_010814227.1	neuralized-like protein 4 isoform X5
XM_015458629.2	XP_015314115.1	neuralized-like protein 4 isoform X2
XM_015458631.2	XP_015314117.1	neuralized-like protein 4 isoform X4
XM_010815925.3	XP_010814227.1	neuralized-like protein 4 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa239128	NM_001192661.3 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226411	XM_005220232.4 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226412	XM_015458629.2 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226413	XM_015458630.2 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226414	XM_015458631.2 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226415	XM_010815925.3 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa216367	NM_001192661.2 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa40828	NM_001192661.1 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29653	XM_005220232.3 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa51581	XM_010815925.2 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa93810	XM_015458629.1 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa93811	XM_015458630.1 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa93812	XM_015458631.1 Latest version!	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

NEURL4 ( NM_001192661.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179590.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.

Price & Availability↑

CloneID	OBa239128
Clone ID Related Accession (Same CDS sequence)	NM_001192661.3
Accession Version	NM_001192661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On May 30, 2018 this sequence version replaced NM_001192661.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCGGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179590.1
CDS	1..4680
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	NM_001192661.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192661.3

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCGGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_005220232.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220289.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226411
Clone ID Related Accession (Same CDS sequence)	XM_005220232.4
Accession Version	XM_005220232.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220232.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGAAGTGTA TGCAGTAGTG GATCTGTACG GCCAGTGCGT CCAAGTGTCC 
ATCACCAATG CCACCGGCCC CATGGACAAC AGCCTGGCGA CCAGCAACTC CGCCACTGAG 
AAGTCGTTCC CCCTGCACTC CCCAGTGGCT GGCGTGGCCC ACCGATTCCA CAGCATCTGT 
GGCAAGAACA TCGCTCTGGA GGAAGACGGC ACGAGGGCAG TGCGAGCAGC CGGCTACGCC 
CATGGCCTCG TCTTCAGCAC CAAGGAGCTC AAGAGTGAGG AAATCTTCGA GGTGAAGGTG 
GAGGAGCTGG ATGAGAAGTG GGCCGGCTCC CTCCGCCTAG GGCTGACCAC GCTAGCGCCT 
GGGGACATGG GGCCCGGCGC AGGTGGGGGC CCGGGACTGC CTCCCTCCCT GCCAGAGCTC 
CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGCGACGG GCAACTCCAG 
AGGATGAACT ATGGCCGGAA CCTGGAGAGG CTGGGGGTGG GAAGCCGTGT GGGCATTCGC 
CGAGGGGCAG ACGACACAAT GCACATCCTG GTAGATGGAG AGGATATGGG TCCTGCAGCC 
ACCGGCATTG CCAAGAGTGT GTGGGCTGTG TTGGATCTAT ATGGGCCAGT GCGGAGTGTC 
TCTATTGTCA GTTCCACCCG TCTGGATGAG CCAGAAGGCA CTCAGCCTCC TTCCCCCAGC 
TCAGACACCG GCAGTGAGGG TGAGGAGGAC GACGAAGGCG AGGAGCATGG CCTGGAAGGC 
CAGAACCAAG TGGCCATTAT GCCCACAGCC CTTGAGTTCC TGGAGAACCA TGGGAAGAAT 
ATTCTCTTGT CCAATGGGAA CCGTACAGCC ACACGAGTGG CCAGCTACAA CCAGGGCATA 
GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTGCTGGTCC AGGTGCGGAT AGACTTCCTG 
AACCGGCAGT GGACATCTTC CCTTGTCCTG GGAGTCATCA CCTGCCCACC GGAGAGGCTC 
AACTTCCCTG CTTCTGCCTG TGCCCTCAAA CGGGCAGCCT GGCTGTTGCG GGGCCGAGGG 
GTCTTCCACA ATGGCCTCAA GATCTGTGAG AAATTTGGGC CGAATCTGGA CACATGTCCA 
GAAGGCACCA TCCTGGGACT GCGGCTGGAC AGCTCTGGGG GGCTGCACCT CCATATCAAC 
GGGATGGACC AGGGGGTGGC TGTGCCAGAT GTCCCCCAGC CATGCCATGC GCTCGTGGAC 
CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGCCCTG AACCAGGGGC TGCCAGTGGG 
AAAAGTGCTG GAACCCAAGG GGACATGGAG AAAGCTGACA TGGTGGATGG TATCAAGGAG 
AGTGTGTGCT GGGGCCCACC GCCCACCACT AGCCCCCTCA AAAGCTGCGA GTATCACGCC 
CTTTGCTCCC GTTTCCAGGA ACTGCTGCTG CTTCCTGAGG ACTATTTCAT GCCTCCGCCG 
AAGCGTAGCC TGTGCTACTG TGAGTCTTGC CGAAAACTTC GAGGAGATGA GGCCCACAGG 
CGCCGAGGGG AGCCCCCCCG TGAATATGCT CTGCCCTTTG GTTGGTGCAG GTTCAACCTC 
AGGGTGAACC CCCGCCTGGA GGCTGGGACA CTAACCAAGA AGTGGCACAT GGCCTACCAT 
GGAAGCAATG TGGCGGCTGT CCGGAGGGTG CTGGACCGAG GGGAGCTGGG GGCAGGCACT 
GCTTCCATCC TGAGCTGCCG GCCCTTGAAG GGAGAGCCTG GGACAGGGTT TGAGGAGCCT 
GGAGAGAACT GTGCGCCTCC CCGGGAGGAG CAGCCCCCTC CAGTGCTGCT TTCCCCCTCC 
CTCCAATATG CTGGGGCTGA GACCCTGGCA TCCAAAGTGC AATTCCGGGA CCCCAAATCC 
CAGCGGACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGTG TGCGCCCTGG CTCCTACACC 
CCGGGACCCC CTTCTGCGGC CCTCAGAGAG CCTCCCGATC CTCACTTCAG CCCAGCCGAA 
CTTGAGTGGG TGACCAAGGA GAAAGGGGCC ACGCTCCTCT ATGCCCTGCT GGTACGGGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220289.1
CDS	1..4686
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPGKEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_005220232.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220232.4

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGAAGTGTA TGCAGTAGTG GATCTGTACG GCCAGTGCGT CCAAGTGTCC 
ATCACCAATG CCACCGGCCC CATGGACAAC AGCCTGGCGA CCAGCAACTC CGCCACTGAG 
AAGTCGTTCC CCCTGCACTC CCCAGTGGCT GGCGTGGCCC ACCGATTCCA CAGCATCTGT 
GGCAAGAACA TCGCTCTGGA GGAAGACGGC ACGAGGGCAG TGCGAGCAGC CGGCTACGCC 
CATGGCCTCG TCTTCAGCAC CAAGGAGCTC AAGAGTGAGG AAATCTTCGA GGTGAAGGTG 
GAGGAGCTGG ATGAGAAGTG GGCCGGCTCC CTCCGCCTAG GGCTGACCAC GCTAGCGCCT 
GGGGACATGG GGCCCGGCGC AGGTGGGGGC CCGGGACTGC CTCCCTCCCT GCCAGAGCTC 
CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGCGACGG GCAACTCCAG 
AGGATGAACT ATGGCCGGAA CCTGGAGAGG CTGGGGGTGG GAAGCCGTGT GGGCATTCGC 
CGAGGGGCAG ACGACACAAT GCACATCCTG GTAGATGGAG AGGATATGGG TCCTGCAGCC 
ACCGGCATTG CCAAGAGTGT GTGGGCTGTG TTGGATCTAT ATGGGCCAGT GCGGAGTGTC 
TCTATTGTCA GTTCCACCCG TCTGGATGAG CCAGAAGGCA CTCAGCCTCC TTCCCCCAGC 
TCAGACACCG GCAGTGAGGG TGAGGAGGAC GACGAAGGCG AGGAGCATGG CCTGGAAGGC 
CAGAACCAAG TGGCCATTAT GCCCACAGCC CTTGAGTTCC TGGAGAACCA TGGGAAGAAT 
ATTCTCTTGT CCAATGGGAA CCGTACAGCC ACACGAGTGG CCAGCTACAA CCAGGGCATA 
GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTGCTGGTCC AGGTGCGGAT AGACTTCCTG 
AACCGGCAGT GGACATCTTC CCTTGTCCTG GGAGTCATCA CCTGCCCACC GGAGAGGCTC 
AACTTCCCTG CTTCTGCCTG TGCCCTCAAA CGGGCAGCCT GGCTGTTGCG GGGCCGAGGG 
GTCTTCCACA ATGGCCTCAA GATCTGTGAG AAATTTGGGC CGAATCTGGA CACATGTCCA 
GAAGGCACCA TCCTGGGACT GCGGCTGGAC AGCTCTGGGG GGCTGCACCT CCATATCAAC 
GGGATGGACC AGGGGGTGGC TGTGCCAGAT GTCCCCCAGC CATGCCATGC GCTCGTGGAC 
CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGCCCTG AACCAGGGGC TGCCAGTGGG 
AAAAGTGCTG GAACCCAAGG GGACATGGAG AAAGCTGACA TGGTGGATGG TATCAAGGAG 
AGTGTGTGCT GGGGCCCACC GCCCACCACT AGCCCCCTCA AAAGCTGCGA GTATCACGCC 
CTTTGCTCCC GTTTCCAGGA ACTGCTGCTG CTTCCTGAGG ACTATTTCAT GCCTCCGCCG 
AAGCGTAGCC TGTGCTACTG TGAGTCTTGC CGAAAACTTC GAGGAGATGA GGCCCACAGG 
CGCCGAGGGG AGCCCCCCCG TGAATATGCT CTGCCCTTTG GTTGGTGCAG GTTCAACCTC 
AGGGTGAACC CCCGCCTGGA GGCTGGGACA CTAACCAAGA AGTGGCACAT GGCCTACCAT 
GGAAGCAATG TGGCGGCTGT CCGGAGGGTG CTGGACCGAG GGGAGCTGGG GGCAGGCACT 
GCTTCCATCC TGAGCTGCCG GCCCTTGAAG GGAGAGCCTG GGACAGGGTT TGAGGAGCCT 
GGAGAGAACT GTGCGCCTCC CCGGGAGGAG CAGCCCCCTC CAGTGCTGCT TTCCCCCTCC 
CTCCAATATG CTGGGGCTGA GACCCTGGCA TCCAAAGTGC AATTCCGGGA CCCCAAATCC 
CAGCGGACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGTG TGCGCCCTGG CTCCTACACC 
CCGGGACCCC CTTCTGCGGC CCTCAGAGAG CCTCCCGATC CTCACTTCAG CCCAGCCGAA 
CTTGAGTGGG TGACCAAGGA GAAAGGGGCC ACGCTCCTCT ATGCCCTGCT GGTACGGGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_015458629.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314115.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.

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CloneID	OBa226412
Clone ID Related Accession (Same CDS sequence)	XM_015458629.2
Accession Version	XM_015458629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGTGTATGC AGTAGTGGAT CTGTACGGCC AGTGCGTCCA AGTGTCCATC 
ACCAATGCCA CCGGCCCCAT GGACAACAGC CTGGCGACCA GCAACTCCGC CACTGAGAAG 
TCGTTCCCCC TGCACTCCCC AGTGGCTGGC GTGGCCCACC GATTCCACAG CATCTGTGGC 
AAGAACATCG CTCTGGAGGA AGACGGCACG AGGGCAGTGC GAGCAGCCGG CTACGCCCAT 
GGCCTCGTCT TCAGCACCAA GGAGCTCAAG AGTGAGGAAA TCTTCGAGGT GAAGGTGGAG 
GAGCTGGATG AGAAGTGGGC CGGCTCCCTC CGCCTAGGGC TGACCACGCT AGCGCCTGGG 
GACATGGGGC CCGGCGCAGG TGGGGGCCCG GGACTGCCTC CCTCCCTGCC AGAGCTCCGG 
ACAAAGACCA CCTGGATGGT GTCCAGCTGT GAAGTGAGGC GCGACGGGCA ACTCCAGAGG 
ATGAACTATG GCCGGAACCT GGAGAGGCTG GGGGTGGGAA GCCGTGTGGG CATTCGCCGA 
GGGGCAGACG ACACAATGCA CATCCTGGTA GATGGAGAGG ATATGGGTCC TGCAGCCACC 
GGCATTGCCA AGAGTGTGTG GGCTGTGTTG GATCTATATG GGCCAGTGCG GAGTGTCTCT 
ATTGTCAGTT CCACCCGTCT GGATGAGCCA GAAGGCACTC AGCCTCCTTC CCCCAGCTCA 
GACACCGGCA GTGAGGGTGA GGAGGACGAC GAAGGCGAGG AGCATGGCCT GGAAGGCCAG 
AACCAAGTGG CCATTATGCC CACAGCCCTT GAGTTCCTGG AGAACCATGG GAAGAATATT 
CTCTTGTCCA ATGGGAACCG TACAGCCACA CGAGTGGCCA GCTACAACCA GGGCATAGTT 
GTCATCAACC AGCCCCTGGT GCCCCAGCTG CTGGTCCAGG TGCGGATAGA CTTCCTGAAC 
CGGCAGTGGA CATCTTCCCT TGTCCTGGGA GTCATCACCT GCCCACCGGA GAGGCTCAAC 
TTCCCTGCTT CTGCCTGTGC CCTCAAACGG GCAGCCTGGC TGTTGCGGGG CCGAGGGGTC 
TTCCACAATG GCCTCAAGAT CTGTGAGAAA TTTGGGCCGA ATCTGGACAC ATGTCCAGAA 
GGCACCATCC TGGGACTGCG GCTGGACAGC TCTGGGGGGC TGCACCTCCA TATCAACGGG 
ATGGACCAGG GGGTGGCTGT GCCAGATGTC CCCCAGCCAT GCCATGCGCT CGTGGACCTC 
TATGGGCAGT GTGAGCAGGT GACAATCGTG AGCCCTGAAC CAGGGGCTGC CAGTGGGAAA 
AGTGCTGGAA CCCAAGGGGA CATGGAGAAA GCTGACATGG TGGATGGTAT CAAGGAGAGT 
GTGTGCTGGG GCCCACCGCC CACCACTAGC CCCCTCAAAA GCTGCGAGTA TCACGCCCTT 
TGCTCCCGTT TCCAGGAACT GCTGCTGCTT CCTGAGGACT ATTTCATGCC TCCGCCGAAG 
CGTAGCCTGT GCTACTGTGA GTCTTGCCGA AAACTTCGAG GAGATGAGGC CCACAGGCGC 
CGAGGGGAGC CCCCCCGTGA ATATGCTCTG CCCTTTGGTT GGTGCAGGTT CAACCTCAGG 
GTGAACCCCC GCCTGGAGGC TGGGACACTA ACCAAGAAGT GGCACATGGC CTACCATGGA 
AGCAATGTGG CGGCTGTCCG GAGGGTGCTG GACCGAGGGG AGCTGGGGGC AGGCACTGCT 
TCCATCCTGA GCTGCCGGCC CTTGAAGGGA GAGCCTGGGA CAGGGTTTGA GGAGCCTGGA 
GAGAACTGTG CGCCTCCCCG GGAGGAGCAG CCCCCTCCAG TGCTGCTTTC CCCCTCCCTC 
CAATATGCTG GGGCTGAGAC CCTGGCATCC AAAGTGCAAT TCCGGGACCC CAAATCCCAG 
CGGACGCACC AGGCCCAGGT GGCGTTCCAG GTGTGTGTGC GCCCTGGCTC CTACACCCCG 
GGACCCCCTT CTGCGGCCCT CAGAGAGCCT CCCGATCCTC ACTTCAGCCC AGCCGAACTT 
GAGTGGGTGA CCAAGGAGAA AGGGGCCACG CTCCTCTATG CCCTGCTGGT ACGGGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314115.1
CDS	702..5384
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPGKVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_015458629.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458629.2

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGTGTATGC AGTAGTGGAT CTGTACGGCC AGTGCGTCCA AGTGTCCATC 
ACCAATGCCA CCGGCCCCAT GGACAACAGC CTGGCGACCA GCAACTCCGC CACTGAGAAG 
TCGTTCCCCC TGCACTCCCC AGTGGCTGGC GTGGCCCACC GATTCCACAG CATCTGTGGC 
AAGAACATCG CTCTGGAGGA AGACGGCACG AGGGCAGTGC GAGCAGCCGG CTACGCCCAT 
GGCCTCGTCT TCAGCACCAA GGAGCTCAAG AGTGAGGAAA TCTTCGAGGT GAAGGTGGAG 
GAGCTGGATG AGAAGTGGGC CGGCTCCCTC CGCCTAGGGC TGACCACGCT AGCGCCTGGG 
GACATGGGGC CCGGCGCAGG TGGGGGCCCG GGACTGCCTC CCTCCCTGCC AGAGCTCCGG 
ACAAAGACCA CCTGGATGGT GTCCAGCTGT GAAGTGAGGC GCGACGGGCA ACTCCAGAGG 
ATGAACTATG GCCGGAACCT GGAGAGGCTG GGGGTGGGAA GCCGTGTGGG CATTCGCCGA 
GGGGCAGACG ACACAATGCA CATCCTGGTA GATGGAGAGG ATATGGGTCC TGCAGCCACC 
GGCATTGCCA AGAGTGTGTG GGCTGTGTTG GATCTATATG GGCCAGTGCG GAGTGTCTCT 
ATTGTCAGTT CCACCCGTCT GGATGAGCCA GAAGGCACTC AGCCTCCTTC CCCCAGCTCA 
GACACCGGCA GTGAGGGTGA GGAGGACGAC GAAGGCGAGG AGCATGGCCT GGAAGGCCAG 
AACCAAGTGG CCATTATGCC CACAGCCCTT GAGTTCCTGG AGAACCATGG GAAGAATATT 
CTCTTGTCCA ATGGGAACCG TACAGCCACA CGAGTGGCCA GCTACAACCA GGGCATAGTT 
GTCATCAACC AGCCCCTGGT GCCCCAGCTG CTGGTCCAGG TGCGGATAGA CTTCCTGAAC 
CGGCAGTGGA CATCTTCCCT TGTCCTGGGA GTCATCACCT GCCCACCGGA GAGGCTCAAC 
TTCCCTGCTT CTGCCTGTGC CCTCAAACGG GCAGCCTGGC TGTTGCGGGG CCGAGGGGTC 
TTCCACAATG GCCTCAAGAT CTGTGAGAAA TTTGGGCCGA ATCTGGACAC ATGTCCAGAA 
GGCACCATCC TGGGACTGCG GCTGGACAGC TCTGGGGGGC TGCACCTCCA TATCAACGGG 
ATGGACCAGG GGGTGGCTGT GCCAGATGTC CCCCAGCCAT GCCATGCGCT CGTGGACCTC 
TATGGGCAGT GTGAGCAGGT GACAATCGTG AGCCCTGAAC CAGGGGCTGC CAGTGGGAAA 
AGTGCTGGAA CCCAAGGGGA CATGGAGAAA GCTGACATGG TGGATGGTAT CAAGGAGAGT 
GTGTGCTGGG GCCCACCGCC CACCACTAGC CCCCTCAAAA GCTGCGAGTA TCACGCCCTT 
TGCTCCCGTT TCCAGGAACT GCTGCTGCTT CCTGAGGACT ATTTCATGCC TCCGCCGAAG 
CGTAGCCTGT GCTACTGTGA GTCTTGCCGA AAACTTCGAG GAGATGAGGC CCACAGGCGC 
CGAGGGGAGC CCCCCCGTGA ATATGCTCTG CCCTTTGGTT GGTGCAGGTT CAACCTCAGG 
GTGAACCCCC GCCTGGAGGC TGGGACACTA ACCAAGAAGT GGCACATGGC CTACCATGGA 
AGCAATGTGG CGGCTGTCCG GAGGGTGCTG GACCGAGGGG AGCTGGGGGC AGGCACTGCT 
TCCATCCTGA GCTGCCGGCC CTTGAAGGGA GAGCCTGGGA CAGGGTTTGA GGAGCCTGGA 
GAGAACTGTG CGCCTCCCCG GGAGGAGCAG CCCCCTCCAG TGCTGCTTTC CCCCTCCCTC 
CAATATGCTG GGGCTGAGAC CCTGGCATCC AAAGTGCAAT TCCGGGACCC CAAATCCCAG 
CGGACGCACC AGGCCCAGGT GGCGTTCCAG GTGTGTGTGC GCCCTGGCTC CTACACCCCG 
GGACCCCCTT CTGCGGCCCT CAGAGAGCCT CCCGATCCTC ACTTCAGCCC AGCCGAACTT 
GAGTGGGTGA CCAAGGAGAA AGGGGCCACG CTCCTCTATG CCCTGCTGGT ACGGGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_015458630.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314116.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.

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CloneID	OBa226413
Clone ID Related Accession (Same CDS sequence)	XM_015458630.2
Accession Version	XM_015458630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGTGT ATGCAGTAGT GGATCTGTAC GGCCAGTGCG TCCAAGTGTC CATCACCAAT 
GCCACCGGCC CCATGGACAA CAGCCTGGCG ACCAGCAACT CCGCCACTGA GAAGTCGTTC 
CCCCTGCACT CCCCAGTGGC TGGCGTGGCC CACCGATTCC ACAGCATCTG TGGCAAGAAC 
ATCGCTCTGG AGGAAGACGG CACGAGGGCA GTGCGAGCAG CCGGCTACGC CCATGGCCTC 
GTCTTCAGCA CCAAGGAGCT CAAGAGTGAG GAAATCTTCG AGGTGAAGGT GGAGGAGCTG 
GATGAGAAGT GGGCCGGCTC CCTCCGCCTA GGGCTGACCA CGCTAGCGCC TGGGGACATG 
GGGCCCGGCG CAGGTGGGGG CCCGGGACTG CCTCCCTCCC TGCCAGAGCT CCGGACAAAG 
ACCACCTGGA TGGTGTCCAG CTGTGAAGTG AGGCGCGACG GGCAACTCCA GAGGATGAAC 
TATGGCCGGA ACCTGGAGAG GCTGGGGGTG GGAAGCCGTG TGGGCATTCG CCGAGGGGCA 
GACGACACAA TGCACATCCT GGTAGATGGA GAGGATATGG GTCCTGCAGC CACCGGCATT 
GCCAAGAGTG TGTGGGCTGT GTTGGATCTA TATGGGCCAG TGCGGAGTGT CTCTATTGTC 
AGTTCCACCC GTCTGGATGA GCCAGAAGGC ACTCAGCCTC CTTCCCCCAG CTCAGACACC 
GGCAGTGAGG GTGAGGAGGA CGACGAAGGC GAGGAGCATG GCCTGGAAGG CCAGAACCAA 
GTGGCCATTA TGCCCACAGC CCTTGAGTTC CTGGAGAACC ATGGGAAGAA TATTCTCTTG 
TCCAATGGGA ACCGTACAGC CACACGAGTG GCCAGCTACA ACCAGGGCAT AGTTGTCATC 
AACCAGCCCC TGGTGCCCCA GCTGCTGGTC CAGGTGCGGA TAGACTTCCT GAACCGGCAG 
TGGACATCTT CCCTTGTCCT GGGAGTCATC ACCTGCCCAC CGGAGAGGCT CAACTTCCCT 
GCTTCTGCCT GTGCCCTCAA ACGGGCAGCC TGGCTGTTGC GGGGCCGAGG GGTCTTCCAC 
AATGGCCTCA AGATCTGTGA GAAATTTGGG CCGAATCTGG ACACATGTCC AGAAGGCACC 
ATCCTGGGAC TGCGGCTGGA CAGCTCTGGG GGGCTGCACC TCCATATCAA CGGGATGGAC 
CAGGGGGTGG CTGTGCCAGA TGTCCCCCAG CCATGCCATG CGCTCGTGGA CCTCTATGGG 
CAGTGTGAGC AGGTGACAAT CGTGAGCCCT GAACCAGGGG CTGCCAGTGG GAAAAGTGCT 
GGAACCCAAG GGGACATGGA GAAAGCTGAC ATGGTGGATG GTATCAAGGA GAGTGTGTGC 
TGGGGCCCAC CGCCCACCAC TAGCCCCCTC AAAAGCTGCG AGTATCACGC CCTTTGCTCC 
CGTTTCCAGG AACTGCTGCT GCTTCCTGAG GACTATTTCA TGCCTCCGCC GAAGCGTAGC 
CTGTGCTACT GTGAGTCTTG CCGAAAACTT CGAGGAGATG AGGCCCACAG GCGCCGAGGG 
GAGCCCCCCC GTGAATATGC TCTGCCCTTT GGTTGGTGCA GGTTCAACCT CAGGGTGAAC 
CCCCGCCTGG AGGCTGGGAC ACTAACCAAG AAGTGGCACA TGGCCTACCA TGGAAGCAAT 
GTGGCGGCTG TCCGGAGGGT GCTGGACCGA GGGGAGCTGG GGGCAGGCAC TGCTTCCATC 
CTGAGCTGCC GGCCCTTGAA GGGAGAGCCT GGGACAGGGT TTGAGGAGCC TGGAGAGAAC 
TGTGCGCCTC CCCGGGAGGA GCAGCCCCCT CCAGTGCTGC TTTCCCCCTC CCTCCAATAT 
GCTGGGGCTG AGACCCTGGC ATCCAAAGTG CAATTCCGGG ACCCCAAATC CCAGCGGACG 
CACCAGGCCC AGGTGGCGTT CCAGGTGTGT GTGCGCCCTG GCTCCTACAC CCCGGGACCC 
CCTTCTGCGG CCCTCAGAGA GCCTCCCGAT CCTCACTTCA GCCCAGCCGA ACTTGAGTGG 
GTGACCAAGG AGAAAGGGGC CACGCTCCTC TATGCCCTGC TGGTACGGGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314116.1
CDS	702..5378
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_015458630.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458630.2

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCGTCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGTGT ATGCAGTAGT GGATCTGTAC GGCCAGTGCG TCCAAGTGTC CATCACCAAT 
GCCACCGGCC CCATGGACAA CAGCCTGGCG ACCAGCAACT CCGCCACTGA GAAGTCGTTC 
CCCCTGCACT CCCCAGTGGC TGGCGTGGCC CACCGATTCC ACAGCATCTG TGGCAAGAAC 
ATCGCTCTGG AGGAAGACGG CACGAGGGCA GTGCGAGCAG CCGGCTACGC CCATGGCCTC 
GTCTTCAGCA CCAAGGAGCT CAAGAGTGAG GAAATCTTCG AGGTGAAGGT GGAGGAGCTG 
GATGAGAAGT GGGCCGGCTC CCTCCGCCTA GGGCTGACCA CGCTAGCGCC TGGGGACATG 
GGGCCCGGCG CAGGTGGGGG CCCGGGACTG CCTCCCTCCC TGCCAGAGCT CCGGACAAAG 
ACCACCTGGA TGGTGTCCAG CTGTGAAGTG AGGCGCGACG GGCAACTCCA GAGGATGAAC 
TATGGCCGGA ACCTGGAGAG GCTGGGGGTG GGAAGCCGTG TGGGCATTCG CCGAGGGGCA 
GACGACACAA TGCACATCCT GGTAGATGGA GAGGATATGG GTCCTGCAGC CACCGGCATT 
GCCAAGAGTG TGTGGGCTGT GTTGGATCTA TATGGGCCAG TGCGGAGTGT CTCTATTGTC 
AGTTCCACCC GTCTGGATGA GCCAGAAGGC ACTCAGCCTC CTTCCCCCAG CTCAGACACC 
GGCAGTGAGG GTGAGGAGGA CGACGAAGGC GAGGAGCATG GCCTGGAAGG CCAGAACCAA 
GTGGCCATTA TGCCCACAGC CCTTGAGTTC CTGGAGAACC ATGGGAAGAA TATTCTCTTG 
TCCAATGGGA ACCGTACAGC CACACGAGTG GCCAGCTACA ACCAGGGCAT AGTTGTCATC 
AACCAGCCCC TGGTGCCCCA GCTGCTGGTC CAGGTGCGGA TAGACTTCCT GAACCGGCAG 
TGGACATCTT CCCTTGTCCT GGGAGTCATC ACCTGCCCAC CGGAGAGGCT CAACTTCCCT 
GCTTCTGCCT GTGCCCTCAA ACGGGCAGCC TGGCTGTTGC GGGGCCGAGG GGTCTTCCAC 
AATGGCCTCA AGATCTGTGA GAAATTTGGG CCGAATCTGG ACACATGTCC AGAAGGCACC 
ATCCTGGGAC TGCGGCTGGA CAGCTCTGGG GGGCTGCACC TCCATATCAA CGGGATGGAC 
CAGGGGGTGG CTGTGCCAGA TGTCCCCCAG CCATGCCATG CGCTCGTGGA CCTCTATGGG 
CAGTGTGAGC AGGTGACAAT CGTGAGCCCT GAACCAGGGG CTGCCAGTGG GAAAAGTGCT 
GGAACCCAAG GGGACATGGA GAAAGCTGAC ATGGTGGATG GTATCAAGGA GAGTGTGTGC 
TGGGGCCCAC CGCCCACCAC TAGCCCCCTC AAAAGCTGCG AGTATCACGC CCTTTGCTCC 
CGTTTCCAGG AACTGCTGCT GCTTCCTGAG GACTATTTCA TGCCTCCGCC GAAGCGTAGC 
CTGTGCTACT GTGAGTCTTG CCGAAAACTT CGAGGAGATG AGGCCCACAG GCGCCGAGGG 
GAGCCCCCCC GTGAATATGC TCTGCCCTTT GGTTGGTGCA GGTTCAACCT CAGGGTGAAC 
CCCCGCCTGG AGGCTGGGAC ACTAACCAAG AAGTGGCACA TGGCCTACCA TGGAAGCAAT 
GTGGCGGCTG TCCGGAGGGT GCTGGACCGA GGGGAGCTGG GGGCAGGCAC TGCTTCCATC 
CTGAGCTGCC GGCCCTTGAA GGGAGAGCCT GGGACAGGGT TTGAGGAGCC TGGAGAGAAC 
TGTGCGCCTC CCCGGGAGGA GCAGCCCCCT CCAGTGCTGC TTTCCCCCTC CCTCCAATAT 
GCTGGGGCTG AGACCCTGGC ATCCAAAGTG CAATTCCGGG ACCCCAAATC CCAGCGGACG 
CACCAGGCCC AGGTGGCGTT CCAGGTGTGT GTGCGCCCTG GCTCCTACAC CCCGGGACCC 
CCTTCTGCGG CCCTCAGAGA GCCTCCCGAT CCTCACTTCA GCCCAGCCGA ACTTGAGTGG 
GTGACCAAGG AGAAAGGGGC CACGCTCCTC TATGCCCTGC TGGTACGGGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_015458631.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314117.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.

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CloneID	OBa226414
Clone ID Related Accession (Same CDS sequence)	XM_015458631.2
Accession Version	XM_015458631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC GTCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GGTAAAGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCGGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314117.1
CDS	702..5321
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPGKEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_015458631.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458631.2

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC GTCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GGTAAAGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCGGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_010815925.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814227.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA.

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CloneID	OBa226415
Clone ID Related Accession (Same CDS sequence)	XM_010815925.3
Accession Version	XM_010815925.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815925.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC GTCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GAAGTGTATG CAGTAGTGGA TCTGTACGGC CAGTGCGTCC AAGTGTCCAT CACCAATGCC 
ACCGGCCCCA TGGACAACAG CCTGGCGACC AGCAACTCCG CCACTGAGAA GTCGTTCCCC 
CTGCACTCCC CAGTGGCTGG CGTGGCCCAC CGATTCCACA GCATCTGTGG CAAGAACATC 
GCTCTGGAGG AAGACGGCAC GAGGGCAGTG CGAGCAGCCG GCTACGCCCA TGGCCTCGTC 
TTCAGCACCA AGGAGCTCAA GAGTGAGGAA ATCTTCGAGG TGAAGGTGGA GGAGCTGGAT 
GAGAAGTGGG CCGGCTCCCT CCGCCTAGGG CTGACCACGC TAGCGCCTGG GGACATGGGG 
CCCGGCGCAG GTGGGGGCCC GGGACTGCCT CCCTCCCTGC CAGAGCTCCG GACAAAGACC 
ACCTGGATGG TGTCCAGCTG TGAAGTGAGG CGCGACGGGC AACTCCAGAG GATGAACTAT 
GGCCGGAACC TGGAGAGGCT GGGGGTGGGA AGCCGTGTGG GCATTCGCCG AGGGGCAGAC 
GACACAATGC ACATCCTGGT AGATGGAGAG GATATGGGTC CTGCAGCCAC CGGCATTGCC 
AAGAGTGTGT GGGCTGTGTT GGATCTATAT GGGCCAGTGC GGAGTGTCTC TATTGTCAGT 
TCCACCCGTC TGGATGAGCC AGAAGGCACT CAGCCTCCTT CCCCCAGCTC AGACACCGGC 
AGTGAGGGTG AGGAGGACGA CGAAGGCGAG GAGCATGGCC TGGAAGGCCA GAACCAAGTG 
GCCATTATGC CCACAGCCCT TGAGTTCCTG GAGAACCATG GGAAGAATAT TCTCTTGTCC 
AATGGGAACC GTACAGCCAC ACGAGTGGCC AGCTACAACC AGGGCATAGT TGTCATCAAC 
CAGCCCCTGG TGCCCCAGCT GCTGGTCCAG GTGCGGATAG ACTTCCTGAA CCGGCAGTGG 
ACATCTTCCC TTGTCCTGGG AGTCATCACC TGCCCACCGG AGAGGCTCAA CTTCCCTGCT 
TCTGCCTGTG CCCTCAAACG GGCAGCCTGG CTGTTGCGGG GCCGAGGGGT CTTCCACAAT 
GGCCTCAAGA TCTGTGAGAA ATTTGGGCCG AATCTGGACA CATGTCCAGA AGGCACCATC 
CTGGGACTGC GGCTGGACAG CTCTGGGGGG CTGCACCTCC ATATCAACGG GATGGACCAG 
GGGGTGGCTG TGCCAGATGT CCCCCAGCCA TGCCATGCGC TCGTGGACCT CTATGGGCAG 
TGTGAGCAGG TGACAATCGT GAGCCCTGAA CCAGGGGCTG CCAGTGGGAA AAGTGCTGGA 
ACCCAAGGGG ACATGGAGAA AGCTGACATG GTGGATGGTA TCAAGGAGAG TGTGTGCTGG 
GGCCCACCGC CCACCACTAG CCCCCTCAAA AGCTGCGAGT ATCACGCCCT TTGCTCCCGT 
TTCCAGGAAC TGCTGCTGCT TCCTGAGGAC TATTTCATGC CTCCGCCGAA GCGTAGCCTG 
TGCTACTGTG AGTCTTGCCG AAAACTTCGA GGAGATGAGG CCCACAGGCG CCGAGGGGAG 
CCCCCCCGTG AATATGCTCT GCCCTTTGGT TGGTGCAGGT TCAACCTCAG GGTGAACCCC 
CGCCTGGAGG CTGGGACACT AACCAAGAAG TGGCACATGG CCTACCATGG AAGCAATGTG 
GCGGCTGTCC GGAGGGTGCT GGACCGAGGG GAGCTGGGGG CAGGCACTGC TTCCATCCTG 
AGCTGCCGGC CCTTGAAGGG AGAGCCTGGG ACAGGGTTTG AGGAGCCTGG AGAGAACTGT 
GCGCCTCCCC GGGAGGAGCA GCCCCCTCCA GTGCTGCTTT CCCCCTCCCT CCAATATGCT 
GGGGCTGAGA CCCTGGCATC CAAAGTGCAA TTCCGGGACC CCAAATCCCA GCGGACGCAC 
CAGGCCCAGG TGGCGTTCCA GGTGTGTGTG CGCCCTGGCT CCTACACCCC GGGACCCCCT 
TCTGCGGCCC TCAGAGAGCC TCCCGATCCT CACTTCAGCC CAGCCGAACT TGAGTGGGTG 
ACCAAGGAGA AAGGGGCCAC GCTCCTCTAT GCCCTGCTGG TACGGGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814227.1
CDS	1..4614
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_010815925.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815925.3

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC GTCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GAAGTGTATG CAGTAGTGGA TCTGTACGGC CAGTGCGTCC AAGTGTCCAT CACCAATGCC 
ACCGGCCCCA TGGACAACAG CCTGGCGACC AGCAACTCCG CCACTGAGAA GTCGTTCCCC 
CTGCACTCCC CAGTGGCTGG CGTGGCCCAC CGATTCCACA GCATCTGTGG CAAGAACATC 
GCTCTGGAGG AAGACGGCAC GAGGGCAGTG CGAGCAGCCG GCTACGCCCA TGGCCTCGTC 
TTCAGCACCA AGGAGCTCAA GAGTGAGGAA ATCTTCGAGG TGAAGGTGGA GGAGCTGGAT 
GAGAAGTGGG CCGGCTCCCT CCGCCTAGGG CTGACCACGC TAGCGCCTGG GGACATGGGG 
CCCGGCGCAG GTGGGGGCCC GGGACTGCCT CCCTCCCTGC CAGAGCTCCG GACAAAGACC 
ACCTGGATGG TGTCCAGCTG TGAAGTGAGG CGCGACGGGC AACTCCAGAG GATGAACTAT 
GGCCGGAACC TGGAGAGGCT GGGGGTGGGA AGCCGTGTGG GCATTCGCCG AGGGGCAGAC 
GACACAATGC ACATCCTGGT AGATGGAGAG GATATGGGTC CTGCAGCCAC CGGCATTGCC 
AAGAGTGTGT GGGCTGTGTT GGATCTATAT GGGCCAGTGC GGAGTGTCTC TATTGTCAGT 
TCCACCCGTC TGGATGAGCC AGAAGGCACT CAGCCTCCTT CCCCCAGCTC AGACACCGGC 
AGTGAGGGTG AGGAGGACGA CGAAGGCGAG GAGCATGGCC TGGAAGGCCA GAACCAAGTG 
GCCATTATGC CCACAGCCCT TGAGTTCCTG GAGAACCATG GGAAGAATAT TCTCTTGTCC 
AATGGGAACC GTACAGCCAC ACGAGTGGCC AGCTACAACC AGGGCATAGT TGTCATCAAC 
CAGCCCCTGG TGCCCCAGCT GCTGGTCCAG GTGCGGATAG ACTTCCTGAA CCGGCAGTGG 
ACATCTTCCC TTGTCCTGGG AGTCATCACC TGCCCACCGG AGAGGCTCAA CTTCCCTGCT 
TCTGCCTGTG CCCTCAAACG GGCAGCCTGG CTGTTGCGGG GCCGAGGGGT CTTCCACAAT 
GGCCTCAAGA TCTGTGAGAA ATTTGGGCCG AATCTGGACA CATGTCCAGA AGGCACCATC 
CTGGGACTGC GGCTGGACAG CTCTGGGGGG CTGCACCTCC ATATCAACGG GATGGACCAG 
GGGGTGGCTG TGCCAGATGT CCCCCAGCCA TGCCATGCGC TCGTGGACCT CTATGGGCAG 
TGTGAGCAGG TGACAATCGT GAGCCCTGAA CCAGGGGCTG CCAGTGGGAA AAGTGCTGGA 
ACCCAAGGGG ACATGGAGAA AGCTGACATG GTGGATGGTA TCAAGGAGAG TGTGTGCTGG 
GGCCCACCGC CCACCACTAG CCCCCTCAAA AGCTGCGAGT ATCACGCCCT TTGCTCCCGT 
TTCCAGGAAC TGCTGCTGCT TCCTGAGGAC TATTTCATGC CTCCGCCGAA GCGTAGCCTG 
TGCTACTGTG AGTCTTGCCG AAAACTTCGA GGAGATGAGG CCCACAGGCG CCGAGGGGAG 
CCCCCCCGTG AATATGCTCT GCCCTTTGGT TGGTGCAGGT TCAACCTCAG GGTGAACCCC 
CGCCTGGAGG CTGGGACACT AACCAAGAAG TGGCACATGG CCTACCATGG AAGCAATGTG 
GCGGCTGTCC GGAGGGTGCT GGACCGAGGG GAGCTGGGGG CAGGCACTGC TTCCATCCTG 
AGCTGCCGGC CCTTGAAGGG AGAGCCTGGG ACAGGGTTTG AGGAGCCTGG AGAGAACTGT 
GCGCCTCCCC GGGAGGAGCA GCCCCCTCCA GTGCTGCTTT CCCCCTCCCT CCAATATGCT 
GGGGCTGAGA CCCTGGCATC CAAAGTGCAA TTCCGGGACC CCAAATCCCA GCGGACGCAC 
CAGGCCCAGG TGGCGTTCCA GGTGTGTGTG CGCCCTGGCT CCTACACCCC GGGACCCCCT 
TCTGCGGCCC TCAGAGAGCC TCCCGATCCT CACTTCAGCC CAGCCGAACT TGAGTGGGTG 
ACCAAGGAGA AAGGGGCCAC GCTCCTCTAT GCCCTGCTGG TACGGGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( NM_001192661.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179590.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.

Price & Availability↑

CloneID	OBa216367
Clone ID Related Accession (Same CDS sequence)	NM_001192661.2
Accession Version	NM_001192661.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02048768.1. On Jun 30, 2017 this sequence version replaced NM_001192661.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCAGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179590.1
CDS	1..4680
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	NM_001192661.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192661.2

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCAGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( NM_001192661.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179590.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.

Price & Availability↑

CloneID	OBa40828
Clone ID Related Accession (Same CDS sequence)	NM_001192661.1
Accession Version	NM_001192661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	neuralized-like protein 4
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03014643.1. On Jul 17, 2010 this sequence version replaced XM_606908.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCGGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179590.1
CDS	1..4680
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	NM_001192661.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192661.1

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCGGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_005220232.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220289.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.

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CloneID	OBa29653
Clone ID Related Accession (Same CDS sequence)	XM_005220232.3
Accession Version	XM_005220232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGAAGTGTA TGCAGTAGTG GATCTGTACG GCCAGTGCGT CCAAGTGTCC 
ATCACCAATG CCACCGGCCC CATGGACAAC AGCCTGGCGA CCAGCAACTC CGCCACTGAG 
AAGTCGTTCC CCCTGCACTC CCCAGTGGCT GGCGTGGCCC ACCGATTCCA CAGCATCTGT 
GGCAAGAACA TCGCTCTGGA GGAAGACGGC ACGAGGGCAG TGCGAGCAGC CGGCTACGCC 
CATGGCCTCG TCTTCAGCAC CAAGGAGCTC AAGAGTGAGG AAATCTTCGA GGTGAAGGTG 
GAGGAGCTGG ATGAGAAGTG GGCCGGCTCC CTCCGCCTAG GGCTGACCAC GCTAGCGCCT 
GGGGACATGG GGCCCGGCGC AGGTGGGGGC CCGGGACTGC CTCCCTCCCT GCCAGAGCTC 
CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGCGACGG GCAACTCCAG 
AGGATGAACT ATGGCCGGAA CCTGGAGAGG CTGGGGGTGG GAAGCCGTGT GGGCATTCGC 
CGAGGGGCAG ACGACACAAT GCACATCCTG GTAGATGGAG AGGATATGGG TCCTGCAGCC 
ACCGGCATTG CCAAGAGTGT GTGGGCTGTG TTGGATCTAT ATGGGCCAGT GCGGAGTGTC 
TCTATTGTCA GTTCCACCCG TCTGGATGAG CCAGAAGGCA CTCAGCCTCC TTCCCCCAGC 
TCAGACACCG GCAGTGAGGG TGAGGAGGAC GACGAAGGCG AGGAGCATGG CCTGGAAGGC 
CAGAACCAAG TGGCCATTAT GCCCACAGCC CTTGAGTTCC TGGAGAACCA TGGGAAGAAT 
ATTCTCTTGT CCAATGGGAA CCGTACAGCC ACACGAGTGG CCAGCTACAA CCAGGGCATA 
GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTGCTGGTCC AGGTGCGGAT AGACTTCCTG 
AACCGGCAGT GGACATCTTC CCTTGTCCTG GGAGTCATCA CCTGCCCACC AGAGAGGCTC 
AACTTCCCTG CTTCTGCCTG TGCCCTCAAA CGGGCAGCCT GGCTGTTGCG GGGCCGAGGG 
GTCTTCCACA ATGGCCTCAA GATCTGTGAG AAATTTGGGC CGAATCTGGA CACATGTCCA 
GAAGGCACCA TCCTGGGACT GCGGCTGGAC AGCTCTGGGG GGCTGCACCT CCATATCAAC 
GGGATGGACC AGGGGGTGGC TGTGCCAGAT GTCCCCCAGC CATGCCATGC GCTCGTGGAC 
CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGCCCTG AACCAGGGGC TGCCAGTGGG 
AAAAGTGCTG GAACCCAAGG GGACATGGAG AAAGCTGACA TGGTGGATGG TATCAAGGAG 
AGTGTGTGCT GGGGCCCACC GCCCACCACT AGCCCCCTCA AAAGCTGCGA GTATCACGCC 
CTTTGCTCCC GTTTCCAGGA ACTGCTGCTG CTTCCTGAGG ACTATTTCAT GCCTCCGCCG 
AAGCGTAGCC TGTGCTACTG TGAGTCTTGC CGAAAACTTC GAGGAGATGA GGCCCACAGG 
CGCCGAGGGG AGCCCCCCCG TGAATATGCT CTGCCCTTTG GTTGGTGCAG GTTCAACCTC 
AGGGTGAACC CCCGCCTGGA GGCTGGGACA CTAACCAAGA AGTGGCACAT GGCCTACCAT 
GGAAGCAATG TGGCGGCTGT CCGGAGGGTG CTGGACCGAG GGGAGCTGGG GGCAGGCACT 
GCTTCCATCC TGAGCTGCCG GCCCTTGAAG GGAGAGCCTG GGACAGGGTT TGAGGAGCCT 
GGAGAGAACT GTGCGCCTCC CCGGGAGGAG CAGCCCCCTC CAGTGCTGCT TTCCCCCTCC 
CTCCAATATG CTGGGGCTGA GACCCTGGCA TCCAAAGTGC AATTCCGGGA CCCCAAATCC 
CAGCGGACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGTG TGCGCCCTGG CTCCTACACC 
CCGGGACCCC CTTCTGCGGC CCTCAGAGAG CCTCCCGATC CTCACTTCAG CCCAGCCGAA 
CTTGAGTGGG TGACCAAGGA GAAAGGGGCC ACGCTCCTCT ATGCCCTGCT GGTACGGGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220289.1
CDS	1..4686
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPGKEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_005220232.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220232.3

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGAAGTGTA TGCAGTAGTG GATCTGTACG GCCAGTGCGT CCAAGTGTCC 
ATCACCAATG CCACCGGCCC CATGGACAAC AGCCTGGCGA CCAGCAACTC CGCCACTGAG 
AAGTCGTTCC CCCTGCACTC CCCAGTGGCT GGCGTGGCCC ACCGATTCCA CAGCATCTGT 
GGCAAGAACA TCGCTCTGGA GGAAGACGGC ACGAGGGCAG TGCGAGCAGC CGGCTACGCC 
CATGGCCTCG TCTTCAGCAC CAAGGAGCTC AAGAGTGAGG AAATCTTCGA GGTGAAGGTG 
GAGGAGCTGG ATGAGAAGTG GGCCGGCTCC CTCCGCCTAG GGCTGACCAC GCTAGCGCCT 
GGGGACATGG GGCCCGGCGC AGGTGGGGGC CCGGGACTGC CTCCCTCCCT GCCAGAGCTC 
CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGCGACGG GCAACTCCAG 
AGGATGAACT ATGGCCGGAA CCTGGAGAGG CTGGGGGTGG GAAGCCGTGT GGGCATTCGC 
CGAGGGGCAG ACGACACAAT GCACATCCTG GTAGATGGAG AGGATATGGG TCCTGCAGCC 
ACCGGCATTG CCAAGAGTGT GTGGGCTGTG TTGGATCTAT ATGGGCCAGT GCGGAGTGTC 
TCTATTGTCA GTTCCACCCG TCTGGATGAG CCAGAAGGCA CTCAGCCTCC TTCCCCCAGC 
TCAGACACCG GCAGTGAGGG TGAGGAGGAC GACGAAGGCG AGGAGCATGG CCTGGAAGGC 
CAGAACCAAG TGGCCATTAT GCCCACAGCC CTTGAGTTCC TGGAGAACCA TGGGAAGAAT 
ATTCTCTTGT CCAATGGGAA CCGTACAGCC ACACGAGTGG CCAGCTACAA CCAGGGCATA 
GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTGCTGGTCC AGGTGCGGAT AGACTTCCTG 
AACCGGCAGT GGACATCTTC CCTTGTCCTG GGAGTCATCA CCTGCCCACC AGAGAGGCTC 
AACTTCCCTG CTTCTGCCTG TGCCCTCAAA CGGGCAGCCT GGCTGTTGCG GGGCCGAGGG 
GTCTTCCACA ATGGCCTCAA GATCTGTGAG AAATTTGGGC CGAATCTGGA CACATGTCCA 
GAAGGCACCA TCCTGGGACT GCGGCTGGAC AGCTCTGGGG GGCTGCACCT CCATATCAAC 
GGGATGGACC AGGGGGTGGC TGTGCCAGAT GTCCCCCAGC CATGCCATGC GCTCGTGGAC 
CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGCCCTG AACCAGGGGC TGCCAGTGGG 
AAAAGTGCTG GAACCCAAGG GGACATGGAG AAAGCTGACA TGGTGGATGG TATCAAGGAG 
AGTGTGTGCT GGGGCCCACC GCCCACCACT AGCCCCCTCA AAAGCTGCGA GTATCACGCC 
CTTTGCTCCC GTTTCCAGGA ACTGCTGCTG CTTCCTGAGG ACTATTTCAT GCCTCCGCCG 
AAGCGTAGCC TGTGCTACTG TGAGTCTTGC CGAAAACTTC GAGGAGATGA GGCCCACAGG 
CGCCGAGGGG AGCCCCCCCG TGAATATGCT CTGCCCTTTG GTTGGTGCAG GTTCAACCTC 
AGGGTGAACC CCCGCCTGGA GGCTGGGACA CTAACCAAGA AGTGGCACAT GGCCTACCAT 
GGAAGCAATG TGGCGGCTGT CCGGAGGGTG CTGGACCGAG GGGAGCTGGG GGCAGGCACT 
GCTTCCATCC TGAGCTGCCG GCCCTTGAAG GGAGAGCCTG GGACAGGGTT TGAGGAGCCT 
GGAGAGAACT GTGCGCCTCC CCGGGAGGAG CAGCCCCCTC CAGTGCTGCT TTCCCCCTCC 
CTCCAATATG CTGGGGCTGA GACCCTGGCA TCCAAAGTGC AATTCCGGGA CCCCAAATCC 
CAGCGGACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGTG TGCGCCCTGG CTCCTACACC 
CCGGGACCCC CTTCTGCGGC CCTCAGAGAG CCTCCCGATC CTCACTTCAG CCCAGCCGAA 
CTTGAGTGGG TGACCAAGGA GAAAGGGGCC ACGCTCCTCT ATGCCCTGCT GGTACGGGTG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_010815925.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814227.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA.

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CloneID	OBa51581
Clone ID Related Accession (Same CDS sequence)	XM_010815925.2
Accession Version	XM_010815925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC ATCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GAAGTGTATG CAGTAGTGGA TCTGTACGGC CAGTGCGTCC AAGTGTCCAT CACCAATGCC 
ACCGGCCCCA TGGACAACAG CCTGGCGACC AGCAACTCCG CCACTGAGAA GTCGTTCCCC 
CTGCACTCCC CAGTGGCTGG CGTGGCCCAC CGATTCCACA GCATCTGTGG CAAGAACATC 
GCTCTGGAGG AAGACGGCAC GAGGGCAGTG CGAGCAGCCG GCTACGCCCA TGGCCTCGTC 
TTCAGCACCA AGGAGCTCAA GAGTGAGGAA ATCTTCGAGG TGAAGGTGGA GGAGCTGGAT 
GAGAAGTGGG CCGGCTCCCT CCGCCTAGGG CTGACCACGC TAGCGCCTGG GGACATGGGG 
CCCGGCGCAG GTGGGGGCCC GGGACTGCCT CCCTCCCTGC CAGAGCTCCG GACAAAGACC 
ACCTGGATGG TGTCCAGCTG TGAAGTGAGG CGCGACGGGC AACTCCAGAG GATGAACTAT 
GGCCGGAACC TGGAGAGGCT GGGGGTGGGA AGCCGTGTGG GCATTCGCCG AGGGGCAGAC 
GACACAATGC ACATCCTGGT AGATGGAGAG GATATGGGTC CTGCAGCCAC CGGCATTGCC 
AAGAGTGTGT GGGCTGTGTT GGATCTATAT GGGCCAGTGC GGAGTGTCTC TATTGTCAGT 
TCCACCCGTC TGGATGAGCC AGAAGGCACT CAGCCTCCTT CCCCCAGCTC AGACACCGGC 
AGTGAGGGTG AGGAGGACGA CGAAGGCGAG GAGCATGGCC TGGAAGGCCA GAACCAAGTG 
GCCATTATGC CCACAGCCCT TGAGTTCCTG GAGAACCATG GGAAGAATAT TCTCTTGTCC 
AATGGGAACC GTACAGCCAC ACGAGTGGCC AGCTACAACC AGGGCATAGT TGTCATCAAC 
CAGCCCCTGG TGCCCCAGCT GCTGGTCCAG GTGCGGATAG ACTTCCTGAA CCGGCAGTGG 
ACATCTTCCC TTGTCCTGGG AGTCATCACC TGCCCACCAG AGAGGCTCAA CTTCCCTGCT 
TCTGCCTGTG CCCTCAAACG GGCAGCCTGG CTGTTGCGGG GCCGAGGGGT CTTCCACAAT 
GGCCTCAAGA TCTGTGAGAA ATTTGGGCCG AATCTGGACA CATGTCCAGA AGGCACCATC 
CTGGGACTGC GGCTGGACAG CTCTGGGGGG CTGCACCTCC ATATCAACGG GATGGACCAG 
GGGGTGGCTG TGCCAGATGT CCCCCAGCCA TGCCATGCGC TCGTGGACCT CTATGGGCAG 
TGTGAGCAGG TGACAATCGT GAGCCCTGAA CCAGGGGCTG CCAGTGGGAA AAGTGCTGGA 
ACCCAAGGGG ACATGGAGAA AGCTGACATG GTGGATGGTA TCAAGGAGAG TGTGTGCTGG 
GGCCCACCGC CCACCACTAG CCCCCTCAAA AGCTGCGAGT ATCACGCCCT TTGCTCCCGT 
TTCCAGGAAC TGCTGCTGCT TCCTGAGGAC TATTTCATGC CTCCGCCGAA GCGTAGCCTG 
TGCTACTGTG AGTCTTGCCG AAAACTTCGA GGAGATGAGG CCCACAGGCG CCGAGGGGAG 
CCCCCCCGTG AATATGCTCT GCCCTTTGGT TGGTGCAGGT TCAACCTCAG GGTGAACCCC 
CGCCTGGAGG CTGGGACACT AACCAAGAAG TGGCACATGG CCTACCATGG AAGCAATGTG 
GCGGCTGTCC GGAGGGTGCT GGACCGAGGG GAGCTGGGGG CAGGCACTGC TTCCATCCTG 
AGCTGCCGGC CCTTGAAGGG AGAGCCTGGG ACAGGGTTTG AGGAGCCTGG AGAGAACTGT 
GCGCCTCCCC GGGAGGAGCA GCCCCCTCCA GTGCTGCTTT CCCCCTCCCT CCAATATGCT 
GGGGCTGAGA CCCTGGCATC CAAAGTGCAA TTCCGGGACC CCAAATCCCA GCGGACGCAC 
CAGGCCCAGG TGGCGTTCCA GGTGTGTGTG CGCCCTGGCT CCTACACCCC GGGACCCCCT 
TCTGCGGCCC TCAGAGAGCC TCCCGATCCT CACTTCAGCC CAGCCGAACT TGAGTGGGTG 
ACCAAGGAGA AAGGGGCCAC GCTCCTCTAT GCCCTGCTGG TACGGGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814227.1
CDS	1..4614
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_010815925.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815925.2

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC ATCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GAAGTGTATG CAGTAGTGGA TCTGTACGGC CAGTGCGTCC AAGTGTCCAT CACCAATGCC 
ACCGGCCCCA TGGACAACAG CCTGGCGACC AGCAACTCCG CCACTGAGAA GTCGTTCCCC 
CTGCACTCCC CAGTGGCTGG CGTGGCCCAC CGATTCCACA GCATCTGTGG CAAGAACATC 
GCTCTGGAGG AAGACGGCAC GAGGGCAGTG CGAGCAGCCG GCTACGCCCA TGGCCTCGTC 
TTCAGCACCA AGGAGCTCAA GAGTGAGGAA ATCTTCGAGG TGAAGGTGGA GGAGCTGGAT 
GAGAAGTGGG CCGGCTCCCT CCGCCTAGGG CTGACCACGC TAGCGCCTGG GGACATGGGG 
CCCGGCGCAG GTGGGGGCCC GGGACTGCCT CCCTCCCTGC CAGAGCTCCG GACAAAGACC 
ACCTGGATGG TGTCCAGCTG TGAAGTGAGG CGCGACGGGC AACTCCAGAG GATGAACTAT 
GGCCGGAACC TGGAGAGGCT GGGGGTGGGA AGCCGTGTGG GCATTCGCCG AGGGGCAGAC 
GACACAATGC ACATCCTGGT AGATGGAGAG GATATGGGTC CTGCAGCCAC CGGCATTGCC 
AAGAGTGTGT GGGCTGTGTT GGATCTATAT GGGCCAGTGC GGAGTGTCTC TATTGTCAGT 
TCCACCCGTC TGGATGAGCC AGAAGGCACT CAGCCTCCTT CCCCCAGCTC AGACACCGGC 
AGTGAGGGTG AGGAGGACGA CGAAGGCGAG GAGCATGGCC TGGAAGGCCA GAACCAAGTG 
GCCATTATGC CCACAGCCCT TGAGTTCCTG GAGAACCATG GGAAGAATAT TCTCTTGTCC 
AATGGGAACC GTACAGCCAC ACGAGTGGCC AGCTACAACC AGGGCATAGT TGTCATCAAC 
CAGCCCCTGG TGCCCCAGCT GCTGGTCCAG GTGCGGATAG ACTTCCTGAA CCGGCAGTGG 
ACATCTTCCC TTGTCCTGGG AGTCATCACC TGCCCACCAG AGAGGCTCAA CTTCCCTGCT 
TCTGCCTGTG CCCTCAAACG GGCAGCCTGG CTGTTGCGGG GCCGAGGGGT CTTCCACAAT 
GGCCTCAAGA TCTGTGAGAA ATTTGGGCCG AATCTGGACA CATGTCCAGA AGGCACCATC 
CTGGGACTGC GGCTGGACAG CTCTGGGGGG CTGCACCTCC ATATCAACGG GATGGACCAG 
GGGGTGGCTG TGCCAGATGT CCCCCAGCCA TGCCATGCGC TCGTGGACCT CTATGGGCAG 
TGTGAGCAGG TGACAATCGT GAGCCCTGAA CCAGGGGCTG CCAGTGGGAA AAGTGCTGGA 
ACCCAAGGGG ACATGGAGAA AGCTGACATG GTGGATGGTA TCAAGGAGAG TGTGTGCTGG 
GGCCCACCGC CCACCACTAG CCCCCTCAAA AGCTGCGAGT ATCACGCCCT TTGCTCCCGT 
TTCCAGGAAC TGCTGCTGCT TCCTGAGGAC TATTTCATGC CTCCGCCGAA GCGTAGCCTG 
TGCTACTGTG AGTCTTGCCG AAAACTTCGA GGAGATGAGG CCCACAGGCG CCGAGGGGAG 
CCCCCCCGTG AATATGCTCT GCCCTTTGGT TGGTGCAGGT TCAACCTCAG GGTGAACCCC 
CGCCTGGAGG CTGGGACACT AACCAAGAAG TGGCACATGG CCTACCATGG AAGCAATGTG 
GCGGCTGTCC GGAGGGTGCT GGACCGAGGG GAGCTGGGGG CAGGCACTGC TTCCATCCTG 
AGCTGCCGGC CCTTGAAGGG AGAGCCTGGG ACAGGGTTTG AGGAGCCTGG AGAGAACTGT 
GCGCCTCCCC GGGAGGAGCA GCCCCCTCCA GTGCTGCTTT CCCCCTCCCT CCAATATGCT 
GGGGCTGAGA CCCTGGCATC CAAAGTGCAA TTCCGGGACC CCAAATCCCA GCGGACGCAC 
CAGGCCCAGG TGGCGTTCCA GGTGTGTGTG CGCCCTGGCT CCTACACCCC GGGACCCCCT 
TCTGCGGCCC TCAGAGAGCC TCCCGATCCT CACTTCAGCC CAGCCGAACT TGAGTGGGTG 
ACCAAGGAGA AAGGGGCCAC GCTCCTCTAT GCCCTGCTGG TACGGGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_015458629.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314115.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.

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CloneID	OBa93810
Clone ID Related Accession (Same CDS sequence)	XM_015458629.1
Accession Version	XM_015458629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGTGTATGC AGTAGTGGAT CTGTACGGCC AGTGCGTCCA AGTGTCCATC 
ACCAATGCCA CCGGCCCCAT GGACAACAGC CTGGCGACCA GCAACTCCGC CACTGAGAAG 
TCGTTCCCCC TGCACTCCCC AGTGGCTGGC GTGGCCCACC GATTCCACAG CATCTGTGGC 
AAGAACATCG CTCTGGAGGA AGACGGCACG AGGGCAGTGC GAGCAGCCGG CTACGCCCAT 
GGCCTCGTCT TCAGCACCAA GGAGCTCAAG AGTGAGGAAA TCTTCGAGGT GAAGGTGGAG 
GAGCTGGATG AGAAGTGGGC CGGCTCCCTC CGCCTAGGGC TGACCACGCT AGCGCCTGGG 
GACATGGGGC CCGGCGCAGG TGGGGGCCCG GGACTGCCTC CCTCCCTGCC AGAGCTCCGG 
ACAAAGACCA CCTGGATGGT GTCCAGCTGT GAAGTGAGGC GCGACGGGCA ACTCCAGAGG 
ATGAACTATG GCCGGAACCT GGAGAGGCTG GGGGTGGGAA GCCGTGTGGG CATTCGCCGA 
GGGGCAGACG ACACAATGCA CATCCTGGTA GATGGAGAGG ATATGGGTCC TGCAGCCACC 
GGCATTGCCA AGAGTGTGTG GGCTGTGTTG GATCTATATG GGCCAGTGCG GAGTGTCTCT 
ATTGTCAGTT CCACCCGTCT GGATGAGCCA GAAGGCACTC AGCCTCCTTC CCCCAGCTCA 
GACACCGGCA GTGAGGGTGA GGAGGACGAC GAAGGCGAGG AGCATGGCCT GGAAGGCCAG 
AACCAAGTGG CCATTATGCC CACAGCCCTT GAGTTCCTGG AGAACCATGG GAAGAATATT 
CTCTTGTCCA ATGGGAACCG TACAGCCACA CGAGTGGCCA GCTACAACCA GGGCATAGTT 
GTCATCAACC AGCCCCTGGT GCCCCAGCTG CTGGTCCAGG TGCGGATAGA CTTCCTGAAC 
CGGCAGTGGA CATCTTCCCT TGTCCTGGGA GTCATCACCT GCCCACCAGA GAGGCTCAAC 
TTCCCTGCTT CTGCCTGTGC CCTCAAACGG GCAGCCTGGC TGTTGCGGGG CCGAGGGGTC 
TTCCACAATG GCCTCAAGAT CTGTGAGAAA TTTGGGCCGA ATCTGGACAC ATGTCCAGAA 
GGCACCATCC TGGGACTGCG GCTGGACAGC TCTGGGGGGC TGCACCTCCA TATCAACGGG 
ATGGACCAGG GGGTGGCTGT GCCAGATGTC CCCCAGCCAT GCCATGCGCT CGTGGACCTC 
TATGGGCAGT GTGAGCAGGT GACAATCGTG AGCCCTGAAC CAGGGGCTGC CAGTGGGAAA 
AGTGCTGGAA CCCAAGGGGA CATGGAGAAA GCTGACATGG TGGATGGTAT CAAGGAGAGT 
GTGTGCTGGG GCCCACCGCC CACCACTAGC CCCCTCAAAA GCTGCGAGTA TCACGCCCTT 
TGCTCCCGTT TCCAGGAACT GCTGCTGCTT CCTGAGGACT ATTTCATGCC TCCGCCGAAG 
CGTAGCCTGT GCTACTGTGA GTCTTGCCGA AAACTTCGAG GAGATGAGGC CCACAGGCGC 
CGAGGGGAGC CCCCCCGTGA ATATGCTCTG CCCTTTGGTT GGTGCAGGTT CAACCTCAGG 
GTGAACCCCC GCCTGGAGGC TGGGACACTA ACCAAGAAGT GGCACATGGC CTACCATGGA 
AGCAATGTGG CGGCTGTCCG GAGGGTGCTG GACCGAGGGG AGCTGGGGGC AGGCACTGCT 
TCCATCCTGA GCTGCCGGCC CTTGAAGGGA GAGCCTGGGA CAGGGTTTGA GGAGCCTGGA 
GAGAACTGTG CGCCTCCCCG GGAGGAGCAG CCCCCTCCAG TGCTGCTTTC CCCCTCCCTC 
CAATATGCTG GGGCTGAGAC CCTGGCATCC AAAGTGCAAT TCCGGGACCC CAAATCCCAG 
CGGACGCACC AGGCCCAGGT GGCGTTCCAG GTGTGTGTGC GCCCTGGCTC CTACACCCCG 
GGACCCCCTT CTGCGGCCCT CAGAGAGCCT CCCGATCCTC ACTTCAGCCC AGCCGAACTT 
GAGTGGGTGA CCAAGGAGAA AGGGGCCACG CTCCTCTATG CCCTGCTGGT ACGGGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314115.1
CDS	265..4947
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPGKVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_015458629.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458629.1

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGGTA AAGTGTATGC AGTAGTGGAT CTGTACGGCC AGTGCGTCCA AGTGTCCATC 
ACCAATGCCA CCGGCCCCAT GGACAACAGC CTGGCGACCA GCAACTCCGC CACTGAGAAG 
TCGTTCCCCC TGCACTCCCC AGTGGCTGGC GTGGCCCACC GATTCCACAG CATCTGTGGC 
AAGAACATCG CTCTGGAGGA AGACGGCACG AGGGCAGTGC GAGCAGCCGG CTACGCCCAT 
GGCCTCGTCT TCAGCACCAA GGAGCTCAAG AGTGAGGAAA TCTTCGAGGT GAAGGTGGAG 
GAGCTGGATG AGAAGTGGGC CGGCTCCCTC CGCCTAGGGC TGACCACGCT AGCGCCTGGG 
GACATGGGGC CCGGCGCAGG TGGGGGCCCG GGACTGCCTC CCTCCCTGCC AGAGCTCCGG 
ACAAAGACCA CCTGGATGGT GTCCAGCTGT GAAGTGAGGC GCGACGGGCA ACTCCAGAGG 
ATGAACTATG GCCGGAACCT GGAGAGGCTG GGGGTGGGAA GCCGTGTGGG CATTCGCCGA 
GGGGCAGACG ACACAATGCA CATCCTGGTA GATGGAGAGG ATATGGGTCC TGCAGCCACC 
GGCATTGCCA AGAGTGTGTG GGCTGTGTTG GATCTATATG GGCCAGTGCG GAGTGTCTCT 
ATTGTCAGTT CCACCCGTCT GGATGAGCCA GAAGGCACTC AGCCTCCTTC CCCCAGCTCA 
GACACCGGCA GTGAGGGTGA GGAGGACGAC GAAGGCGAGG AGCATGGCCT GGAAGGCCAG 
AACCAAGTGG CCATTATGCC CACAGCCCTT GAGTTCCTGG AGAACCATGG GAAGAATATT 
CTCTTGTCCA ATGGGAACCG TACAGCCACA CGAGTGGCCA GCTACAACCA GGGCATAGTT 
GTCATCAACC AGCCCCTGGT GCCCCAGCTG CTGGTCCAGG TGCGGATAGA CTTCCTGAAC 
CGGCAGTGGA CATCTTCCCT TGTCCTGGGA GTCATCACCT GCCCACCAGA GAGGCTCAAC 
TTCCCTGCTT CTGCCTGTGC CCTCAAACGG GCAGCCTGGC TGTTGCGGGG CCGAGGGGTC 
TTCCACAATG GCCTCAAGAT CTGTGAGAAA TTTGGGCCGA ATCTGGACAC ATGTCCAGAA 
GGCACCATCC TGGGACTGCG GCTGGACAGC TCTGGGGGGC TGCACCTCCA TATCAACGGG 
ATGGACCAGG GGGTGGCTGT GCCAGATGTC CCCCAGCCAT GCCATGCGCT CGTGGACCTC 
TATGGGCAGT GTGAGCAGGT GACAATCGTG AGCCCTGAAC CAGGGGCTGC CAGTGGGAAA 
AGTGCTGGAA CCCAAGGGGA CATGGAGAAA GCTGACATGG TGGATGGTAT CAAGGAGAGT 
GTGTGCTGGG GCCCACCGCC CACCACTAGC CCCCTCAAAA GCTGCGAGTA TCACGCCCTT 
TGCTCCCGTT TCCAGGAACT GCTGCTGCTT CCTGAGGACT ATTTCATGCC TCCGCCGAAG 
CGTAGCCTGT GCTACTGTGA GTCTTGCCGA AAACTTCGAG GAGATGAGGC CCACAGGCGC 
CGAGGGGAGC CCCCCCGTGA ATATGCTCTG CCCTTTGGTT GGTGCAGGTT CAACCTCAGG 
GTGAACCCCC GCCTGGAGGC TGGGACACTA ACCAAGAAGT GGCACATGGC CTACCATGGA 
AGCAATGTGG CGGCTGTCCG GAGGGTGCTG GACCGAGGGG AGCTGGGGGC AGGCACTGCT 
TCCATCCTGA GCTGCCGGCC CTTGAAGGGA GAGCCTGGGA CAGGGTTTGA GGAGCCTGGA 
GAGAACTGTG CGCCTCCCCG GGAGGAGCAG CCCCCTCCAG TGCTGCTTTC CCCCTCCCTC 
CAATATGCTG GGGCTGAGAC CCTGGCATCC AAAGTGCAAT TCCGGGACCC CAAATCCCAG 
CGGACGCACC AGGCCCAGGT GGCGTTCCAG GTGTGTGTGC GCCCTGGCTC CTACACCCCG 
GGACCCCCTT CTGCGGCCCT CAGAGAGCCT CCCGATCCTC ACTTCAGCCC AGCCGAACTT 
GAGTGGGTGA CCAAGGAGAA AGGGGCCACG CTCCTCTATG CCCTGCTGGT ACGGGTGGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_015458630.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314116.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa93811
Clone ID Related Accession (Same CDS sequence)	XM_015458630.1
Accession Version	XM_015458630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGTGT ATGCAGTAGT GGATCTGTAC GGCCAGTGCG TCCAAGTGTC CATCACCAAT 
GCCACCGGCC CCATGGACAA CAGCCTGGCG ACCAGCAACT CCGCCACTGA GAAGTCGTTC 
CCCCTGCACT CCCCAGTGGC TGGCGTGGCC CACCGATTCC ACAGCATCTG TGGCAAGAAC 
ATCGCTCTGG AGGAAGACGG CACGAGGGCA GTGCGAGCAG CCGGCTACGC CCATGGCCTC 
GTCTTCAGCA CCAAGGAGCT CAAGAGTGAG GAAATCTTCG AGGTGAAGGT GGAGGAGCTG 
GATGAGAAGT GGGCCGGCTC CCTCCGCCTA GGGCTGACCA CGCTAGCGCC TGGGGACATG 
GGGCCCGGCG CAGGTGGGGG CCCGGGACTG CCTCCCTCCC TGCCAGAGCT CCGGACAAAG 
ACCACCTGGA TGGTGTCCAG CTGTGAAGTG AGGCGCGACG GGCAACTCCA GAGGATGAAC 
TATGGCCGGA ACCTGGAGAG GCTGGGGGTG GGAAGCCGTG TGGGCATTCG CCGAGGGGCA 
GACGACACAA TGCACATCCT GGTAGATGGA GAGGATATGG GTCCTGCAGC CACCGGCATT 
GCCAAGAGTG TGTGGGCTGT GTTGGATCTA TATGGGCCAG TGCGGAGTGT CTCTATTGTC 
AGTTCCACCC GTCTGGATGA GCCAGAAGGC ACTCAGCCTC CTTCCCCCAG CTCAGACACC 
GGCAGTGAGG GTGAGGAGGA CGACGAAGGC GAGGAGCATG GCCTGGAAGG CCAGAACCAA 
GTGGCCATTA TGCCCACAGC CCTTGAGTTC CTGGAGAACC ATGGGAAGAA TATTCTCTTG 
TCCAATGGGA ACCGTACAGC CACACGAGTG GCCAGCTACA ACCAGGGCAT AGTTGTCATC 
AACCAGCCCC TGGTGCCCCA GCTGCTGGTC CAGGTGCGGA TAGACTTCCT GAACCGGCAG 
TGGACATCTT CCCTTGTCCT GGGAGTCATC ACCTGCCCAC CAGAGAGGCT CAACTTCCCT 
GCTTCTGCCT GTGCCCTCAA ACGGGCAGCC TGGCTGTTGC GGGGCCGAGG GGTCTTCCAC 
AATGGCCTCA AGATCTGTGA GAAATTTGGG CCGAATCTGG ACACATGTCC AGAAGGCACC 
ATCCTGGGAC TGCGGCTGGA CAGCTCTGGG GGGCTGCACC TCCATATCAA CGGGATGGAC 
CAGGGGGTGG CTGTGCCAGA TGTCCCCCAG CCATGCCATG CGCTCGTGGA CCTCTATGGG 
CAGTGTGAGC AGGTGACAAT CGTGAGCCCT GAACCAGGGG CTGCCAGTGG GAAAAGTGCT 
GGAACCCAAG GGGACATGGA GAAAGCTGAC ATGGTGGATG GTATCAAGGA GAGTGTGTGC 
TGGGGCCCAC CGCCCACCAC TAGCCCCCTC AAAAGCTGCG AGTATCACGC CCTTTGCTCC 
CGTTTCCAGG AACTGCTGCT GCTTCCTGAG GACTATTTCA TGCCTCCGCC GAAGCGTAGC 
CTGTGCTACT GTGAGTCTTG CCGAAAACTT CGAGGAGATG AGGCCCACAG GCGCCGAGGG 
GAGCCCCCCC GTGAATATGC TCTGCCCTTT GGTTGGTGCA GGTTCAACCT CAGGGTGAAC 
CCCCGCCTGG AGGCTGGGAC ACTAACCAAG AAGTGGCACA TGGCCTACCA TGGAAGCAAT 
GTGGCGGCTG TCCGGAGGGT GCTGGACCGA GGGGAGCTGG GGGCAGGCAC TGCTTCCATC 
CTGAGCTGCC GGCCCTTGAA GGGAGAGCCT GGGACAGGGT TTGAGGAGCC TGGAGAGAAC 
TGTGCGCCTC CCCGGGAGGA GCAGCCCCCT CCAGTGCTGC TTTCCCCCTC CCTCCAATAT 
GCTGGGGCTG AGACCCTGGC ATCCAAAGTG CAATTCCGGG ACCCCAAATC CCAGCGGACG 
CACCAGGCCC AGGTGGCGTT CCAGGTGTGT GTGCGCCCTG GCTCCTACAC CCCGGGACCC 
CCTTCTGCGG CCCTCAGAGA GCCTCCCGAT CCTCACTTCA GCCCAGCCGA ACTTGAGTGG 
GTGACCAAGG AGAAAGGGGC CACGCTCCTC TATGCCCTGC TGGTACGGGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314116.1
CDS	265..4941
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEAFMVSPAQARPETFPNSLESHNDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_015458630.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458630.1

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGCCT TCATGGTATC CCCAGCGCAG GCCCGACCGG AGACGTTTCC TAACAGCCTT 
GAGTCGCATA ATGACTTTGC CGGCATGGAG CTCTCCGAGG TGGTGAGCAA TGCCATCCTG 
TCTGCGTACA ACGGGGGGCT CCTGAATGTG AACCTGAGCT CCCCCCCAGC AGGGGAAGCA 
CCAGGGTCTA GCGGTGCTGC CACCTCGCCC ATTGTCACCT CCAACGATGC TCTCCTTTTC 
CATGAGAAGT GTGGAACCCT CATCAAGCTC AGCAACAATA ATAAGACCGC TGAGCGCCGC 
CGGCCCCTGG ATGAATTCAA CAACGGGGTT GTCATGACCA ATCGCCCGCT CCGGGACAAT 
GAGATGTTTG AGATCCGCAT CGATAAGCTC GTAGATAAGT GGTCAGGCTC CATTGAGATT 
GGTGTCACCA CCCACAACCC CAACAATCTG GAGTACCCAG CCACCATGAC CAACCTGCAG 
TCAGGTACCA TCATGATGAG CGGCTGTGGT ATCCTGACCA ATGGCAAGGG CACCCGCCGG 
GAGTACTGTG AATTCAGCCT GGATGAGCTG CAGGAGGGTG ATCACATTGG TCTCACGAGG 
AAGTCCAACT CTGCCCTACA CTTCTTCATT AATGGCATTG ATCAGGGTGT GGCGACCCCC 
TTGACACCCC CAGTGGTGTA CGGTGTGGTG GACTTGTATG GGATGGCCGT GAAGGTGACC 
ATCGTCCACA ATAACAACCA CAGTGACCGC CTTCGCCGGA ACAATGCCAT CCTGCGGGCT 
CTGTCCCCTG AGGGTGCTCT CCGCCGGGCT GCTCCTACAA CCCAGGCAGA ACCTGAGCGC 
CTGCTCTTCC ACCCCAACTG TGGGCAGAAG GCAGCCATCA CCCACGAGGG ACGCACTGCC 
CTGAGGCCCC ATGCCACCGA CGACTTCAAT CATGGTGTGG TGCTGAGCAG CAGAGCCCTG 
CGGGATGGAG AGGTGTTCCA GGTGCGCATC GACAAGATGG TGGACAAGTG GGCTGGCTCC 
ATTGAGATTG GCGTCACCAC CCACAACCCT GCCTACCTCC AGTTGCCCTC CACCATGACC 
AACTTGCGCT CTGGGACCTG GATGATGACA GGGAATGGGG TGATGCACAA CGGGACGACC 
ATCTTGGACG AATACGGGCA CAACCTGGAC CGACTCAAGG CAGGGGACAC GGTGGGCGTT 
GTGCGGCGGG AGGACGGGAC TCTCCACTTC TTCGTCAATG GCATGACTCA GGGTCCCGCT 
GCCTGGAATG TGCCCCCAGG AGTCTATGCT GTAGTGGATC TCTATGGCCA GGCGGCCCAG 
GCCACCATTG TGGACGACGT GGAGGTGCCC CAAGTCCCTG AACCACTCCC TGAGGGGAAC 
AACCAGGTGT CTCCAAGCTC CCCATCATCT GGGGCTGGGG GCTCGGACCT CCGCTTCCAC 
CAGCTGCATG GCAGCAATGC GGTCATCACC AACGGGGGCC GTACTGCGCT CCGCCACAAT 
TGCCGCAGCG AGTTCAACGA TGCCATTGTC ATCTCCAACC GAGCCCTGAG AGATGGCGAG 
CTGTTTGAAA TCGTCATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA 
GTGACCGCTA TTCGGCCGGA GGACCTTGAA TTCCCCAACA CTATGACAGA CATTGACTAT 
GACACATGGA TGCTGAGCGG CACAGCCATC ATGCAAGATG GGAACACCAT GCGCAACAAC 
TACGGGTGTG ATCTTGACGC GCTGGGCACT GGCGCCCGCA TCGGTATGAT GCGCACGGCC 
AAGGGCGACC TGCACTACTT CATCAATGGC CAGGACCAAG GCGCTGCGTG CTCAGGCTTG 
CCTCCCGTGT ATGCAGTAGT GGATCTGTAC GGCCAGTGCG TCCAAGTGTC CATCACCAAT 
GCCACCGGCC CCATGGACAA CAGCCTGGCG ACCAGCAACT CCGCCACTGA GAAGTCGTTC 
CCCCTGCACT CCCCAGTGGC TGGCGTGGCC CACCGATTCC ACAGCATCTG TGGCAAGAAC 
ATCGCTCTGG AGGAAGACGG CACGAGGGCA GTGCGAGCAG CCGGCTACGC CCATGGCCTC 
GTCTTCAGCA CCAAGGAGCT CAAGAGTGAG GAAATCTTCG AGGTGAAGGT GGAGGAGCTG 
GATGAGAAGT GGGCCGGCTC CCTCCGCCTA GGGCTGACCA CGCTAGCGCC TGGGGACATG 
GGGCCCGGCG CAGGTGGGGG CCCGGGACTG CCTCCCTCCC TGCCAGAGCT CCGGACAAAG 
ACCACCTGGA TGGTGTCCAG CTGTGAAGTG AGGCGCGACG GGCAACTCCA GAGGATGAAC 
TATGGCCGGA ACCTGGAGAG GCTGGGGGTG GGAAGCCGTG TGGGCATTCG CCGAGGGGCA 
GACGACACAA TGCACATCCT GGTAGATGGA GAGGATATGG GTCCTGCAGC CACCGGCATT 
GCCAAGAGTG TGTGGGCTGT GTTGGATCTA TATGGGCCAG TGCGGAGTGT CTCTATTGTC 
AGTTCCACCC GTCTGGATGA GCCAGAAGGC ACTCAGCCTC CTTCCCCCAG CTCAGACACC 
GGCAGTGAGG GTGAGGAGGA CGACGAAGGC GAGGAGCATG GCCTGGAAGG CCAGAACCAA 
GTGGCCATTA TGCCCACAGC CCTTGAGTTC CTGGAGAACC ATGGGAAGAA TATTCTCTTG 
TCCAATGGGA ACCGTACAGC CACACGAGTG GCCAGCTACA ACCAGGGCAT AGTTGTCATC 
AACCAGCCCC TGGTGCCCCA GCTGCTGGTC CAGGTGCGGA TAGACTTCCT GAACCGGCAG 
TGGACATCTT CCCTTGTCCT GGGAGTCATC ACCTGCCCAC CAGAGAGGCT CAACTTCCCT 
GCTTCTGCCT GTGCCCTCAA ACGGGCAGCC TGGCTGTTGC GGGGCCGAGG GGTCTTCCAC 
AATGGCCTCA AGATCTGTGA GAAATTTGGG CCGAATCTGG ACACATGTCC AGAAGGCACC 
ATCCTGGGAC TGCGGCTGGA CAGCTCTGGG GGGCTGCACC TCCATATCAA CGGGATGGAC 
CAGGGGGTGG CTGTGCCAGA TGTCCCCCAG CCATGCCATG CGCTCGTGGA CCTCTATGGG 
CAGTGTGAGC AGGTGACAAT CGTGAGCCCT GAACCAGGGG CTGCCAGTGG GAAAAGTGCT 
GGAACCCAAG GGGACATGGA GAAAGCTGAC ATGGTGGATG GTATCAAGGA GAGTGTGTGC 
TGGGGCCCAC CGCCCACCAC TAGCCCCCTC AAAAGCTGCG AGTATCACGC CCTTTGCTCC 
CGTTTCCAGG AACTGCTGCT GCTTCCTGAG GACTATTTCA TGCCTCCGCC GAAGCGTAGC 
CTGTGCTACT GTGAGTCTTG CCGAAAACTT CGAGGAGATG AGGCCCACAG GCGCCGAGGG 
GAGCCCCCCC GTGAATATGC TCTGCCCTTT GGTTGGTGCA GGTTCAACCT CAGGGTGAAC 
CCCCGCCTGG AGGCTGGGAC ACTAACCAAG AAGTGGCACA TGGCCTACCA TGGAAGCAAT 
GTGGCGGCTG TCCGGAGGGT GCTGGACCGA GGGGAGCTGG GGGCAGGCAC TGCTTCCATC 
CTGAGCTGCC GGCCCTTGAA GGGAGAGCCT GGGACAGGGT TTGAGGAGCC TGGAGAGAAC 
TGTGCGCCTC CCCGGGAGGA GCAGCCCCCT CCAGTGCTGC TTTCCCCCTC CCTCCAATAT 
GCTGGGGCTG AGACCCTGGC ATCCAAAGTG CAATTCCGGG ACCCCAAATC CCAGCGGACG 
CACCAGGCCC AGGTGGCGTT CCAGGTGTGT GTGCGCCCTG GCTCCTACAC CCCGGGACCC 
CCTTCTGCGG CCCTCAGAGA GCCTCCCGAT CCTCACTTCA GCCCAGCCGA ACTTGAGTGG 
GTGACCAAGG AGAAAGGGGC CACGCTCCTC TATGCCCTGC TGGTACGGGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEURL4 ( XM_015458631.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314117.1 Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa93812
Clone ID Related Accession (Same CDS sequence)	XM_015458631.1
Accession Version	XM_015458631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neuralized-like protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC ATCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GGTAAAGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCAGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314117.1
CDS	264..4883
Translation	MAAGSGGNGGSGGGPGPGPGGGGGPGGSGPGPGSGGSLGSSGELHPRTGRLVSLSACGRTARRQQPGQEFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPNVLDFPSSATGLKGGSWVVSGCSVLRDGRSVLEEYGQDLDQLGEGDRVGVERTAAGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPPEPGFSPPTPSPTPPLEPSAPAEDSALAEQGTSGDEDFAGMELSEVVSNAILSAYNGGLLNVNLSSPPAGEAPGSSGAATSPIVTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNNGVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNNLEYPATMTNLQSGTIMMSGCGILTNGKGTRREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHSDRLRRNNAILRALSPEGALRRAAPTTQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSRALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPQVPEPLPEGNNQVSPSSPSSGAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQKMVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMRTAKGDLHYFINGQDQGAACSGLPPGKEVYAVVDLYGQCVQVSITNATGPMDNSLATSNSATEKSFPLHSPVAGVAHRFHSICGKNIALEEDGTRAVRAAGYAHGLVFSTKELKSEEIFEVKVEELDEKWAGSLRLGLTTLAPGDMGPGAGGGPGLPPSLPELRTKTTWMVSSCEVRRDGQLQRMNYGRNLERLGVGSRVGIRRGADDTMHILVDGEDMGPAATGIAKSVWAVLDLYGPVRSVSIVSSTRLDEPEGTQPPSPSSDTGSEGEEDDEGEEHGLEGQNQVAIMPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVINQPLVPQLLVQVRIDFLNRQWTSSLVLGVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHINGMDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPEPGAASGKSAGTQGDMEKADMVDGIKESVCWGPPPTTSPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNLRVNPRLEAGTLTKKWHMAYHGSNVAAVRRVLDRGELGAGTASILSCRPLKGEPGTGFEEPGENCAPPREEQPPPVLLSPSLQYAGAETLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALREPPDPHFSPAELEWVTKEKGATLLYALLVRVE

Target ORF information:

RefSeq Version	XM_015458631.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458631.1

ORF Insert Sequence:

ATGGCGGCGG GGTCGGGTGG GAATGGGGGC TCCGGGGGAG GCCCCGGGCC GGGGCCGGGT 
GGGGGTGGTG GCCCCGGCGG GAGCGGCCCA GGGCCGGGGT CCGGCGGGAG TCTGGGCAGC 
AGCGGGGAGC TGCATCCGCG CACCGGGCGC TTGGTGAGCT TGTCGGCCTG TGGGCGTACG 
GCGCGGCGGC AGCAGCCAGG CCAGGAGTTT AACCACGGGC TGGTGTTGAG TCGGGAACCC 
TTGCGGGATG GACGCGTCTT CACCGTCCGC ATCGACCGCA AGGTCAACTC CTGGAGTGGC 
TCCATTGAGA TTGGGGTGAC GGCACTGGAC CCCAATGTGC TGGACTTCCC AAGCAGCGCT 
ACAGGGCTGA AGGGGGGCTC GTGGGTAGTG TCGGGCTGCT CGGTGCTGAG GGATGGACGG 
TCTGTGCTGG AGGAGTATGG GCAGGACCTT GACCAGCTTG GCGAAGGGGA CCGTGTGGGC 
GTGGAGCGCA CGGCTGCCGG GGAGCTGCGG CTCTGGGTGA ATGGGCGGGA TTGCGGCGTG 
GCTGCCACGG GCCTTCCGGC TCGTGTCTGG GCCGTCGTGG ACCTTTACGG CAAGTGCACC 
CAGATCACTG TGCTCCCCCC TGAGCCGGGC TTCAGCCCCC CTACTCCCAG CCCCACGCCT 
CCCCTTGAGC CTTCCGCCCC CGCTGAAGAT TCTGCCTTGG CTGAACAAGG GACCTCTGGG 
GATGAAGACT TTGCCGGCAT GGAGCTCTCC GAGGTGGTGA GCAATGCCAT CCTGTCTGCG 
TACAACGGGG GGCTCCTGAA TGTGAACCTG AGCTCCCCCC CAGCAGGGGA AGCACCAGGG 
TCTAGCGGTG CTGCCACCTC GCCCATTGTC ACCTCCAACG ATGCTCTCCT TTTCCATGAG 
AAGTGTGGAA CCCTCATCAA GCTCAGCAAC AATAATAAGA CCGCTGAGCG CCGCCGGCCC 
CTGGATGAAT TCAACAACGG GGTTGTCATG ACCAATCGCC CGCTCCGGGA CAATGAGATG 
TTTGAGATCC GCATCGATAA GCTCGTAGAT AAGTGGTCAG GCTCCATTGA GATTGGTGTC 
ACCACCCACA ACCCCAACAA TCTGGAGTAC CCAGCCACCA TGACCAACCT GCAGTCAGGT 
ACCATCATGA TGAGCGGCTG TGGTATCCTG ACCAATGGCA AGGGCACCCG CCGGGAGTAC 
TGTGAATTCA GCCTGGATGA GCTGCAGGAG GGTGATCACA TTGGTCTCAC GAGGAAGTCC 
AACTCTGCCC TACACTTCTT CATTAATGGC ATTGATCAGG GTGTGGCGAC CCCCTTGACA 
CCCCCAGTGG TGTACGGTGT GGTGGACTTG TATGGGATGG CCGTGAAGGT GACCATCGTC 
CACAATAACA ACCACAGTGA CCGCCTTCGC CGGAACAATG CCATCCTGCG GGCTCTGTCC 
CCTGAGGGTG CTCTCCGCCG GGCTGCTCCT ACAACCCAGG CAGAACCTGA GCGCCTGCTC 
TTCCACCCCA ACTGTGGGCA GAAGGCAGCC ATCACCCACG AGGGACGCAC TGCCCTGAGG 
CCCCATGCCA CCGACGACTT CAATCATGGT GTGGTGCTGA GCAGCAGAGC CCTGCGGGAT 
GGAGAGGTGT TCCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG 
ATTGGCGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG 
CGCTCTGGGA CCTGGATGAT GACAGGGAAT GGGGTGATGC ACAACGGGAC GACCATCTTG 
GACGAATACG GGCACAACCT GGACCGACTC AAGGCAGGGG ACACGGTGGG CGTTGTGCGG 
CGGGAGGACG GGACTCTCCA CTTCTTCGTC AATGGCATGA CTCAGGGTCC CGCTGCCTGG 
AATGTGCCCC CAGGAGTCTA TGCTGTAGTG GATCTCTATG GCCAGGCGGC CCAGGCCACC 
ATTGTGGACG ACGTGGAGGT GCCCCAAGTC CCTGAACCAC TCCCTGAGGG GAACAACCAG 
GTGTCTCCAA GCTCCCCATC ATCTGGGGCT GGGGGCTCGG ACCTCCGCTT CCACCAGCTG 
CATGGCAGCA ATGCGGTCAT CACCAACGGG GGCCGTACTG CGCTCCGCCA CAATTGCCGC 
AGCGAGTTCA ACGATGCCAT TGTCATCTCC AACCGAGCCC TGAGAGATGG CGAGCTGTTT 
GAAATCGTCA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACC 
GCTATTCGGC CGGAGGACCT TGAATTCCCC AACACTATGA CAGACATTGA CTATGACACA 
TGGATGCTGA GCGGCACAGC CATCATGCAA GATGGGAACA CCATGCGCAA CAACTACGGG 
TGTGATCTTG ACGCGCTGGG CACTGGCGCC CGCATCGGTA TGATGCGCAC GGCCAAGGGC 
GACCTGCACT ACTTCATCAA TGGCCAGGAC CAAGGCGCTG CGTGCTCAGG CTTGCCTCCC 
GGTAAAGAAG TGTATGCAGT AGTGGATCTG TACGGCCAGT GCGTCCAAGT GTCCATCACC 
AATGCCACCG GCCCCATGGA CAACAGCCTG GCGACCAGCA ACTCCGCCAC TGAGAAGTCG 
TTCCCCCTGC ACTCCCCAGT GGCTGGCGTG GCCCACCGAT TCCACAGCAT CTGTGGCAAG 
AACATCGCTC TGGAGGAAGA CGGCACGAGG GCAGTGCGAG CAGCCGGCTA CGCCCATGGC 
CTCGTCTTCA GCACCAAGGA GCTCAAGAGT GAGGAAATCT TCGAGGTGAA GGTGGAGGAG 
CTGGATGAGA AGTGGGCCGG CTCCCTCCGC CTAGGGCTGA CCACGCTAGC GCCTGGGGAC 
ATGGGGCCCG GCGCAGGTGG GGGCCCGGGA CTGCCTCCCT CCCTGCCAGA GCTCCGGACA 
AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGCG ACGGGCAACT CCAGAGGATG 
AACTATGGCC GGAACCTGGA GAGGCTGGGG GTGGGAAGCC GTGTGGGCAT TCGCCGAGGG 
GCAGACGACA CAATGCACAT CCTGGTAGAT GGAGAGGATA TGGGTCCTGC AGCCACCGGC 
ATTGCCAAGA GTGTGTGGGC TGTGTTGGAT CTATATGGGC CAGTGCGGAG TGTCTCTATT 
GTCAGTTCCA CCCGTCTGGA TGAGCCAGAA GGCACTCAGC CTCCTTCCCC CAGCTCAGAC 
ACCGGCAGTG AGGGTGAGGA GGACGACGAA GGCGAGGAGC ATGGCCTGGA AGGCCAGAAC 
CAAGTGGCCA TTATGCCCAC AGCCCTTGAG TTCCTGGAGA ACCATGGGAA GAATATTCTC 
TTGTCCAATG GGAACCGTAC AGCCACACGA GTGGCCAGCT ACAACCAGGG CATAGTTGTC 
ATCAACCAGC CCCTGGTGCC CCAGCTGCTG GTCCAGGTGC GGATAGACTT CCTGAACCGG 
CAGTGGACAT CTTCCCTTGT CCTGGGAGTC ATCACCTGCC CACCAGAGAG GCTCAACTTC 
CCTGCTTCTG CCTGTGCCCT CAAACGGGCA GCCTGGCTGT TGCGGGGCCG AGGGGTCTTC 
CACAATGGCC TCAAGATCTG TGAGAAATTT GGGCCGAATC TGGACACATG TCCAGAAGGC 
ACCATCCTGG GACTGCGGCT GGACAGCTCT GGGGGGCTGC ACCTCCATAT CAACGGGATG 
GACCAGGGGG TGGCTGTGCC AGATGTCCCC CAGCCATGCC ATGCGCTCGT GGACCTCTAT 
GGGCAGTGTG AGCAGGTGAC AATCGTGAGC CCTGAACCAG GGGCTGCCAG TGGGAAAAGT 
GCTGGAACCC AAGGGGACAT GGAGAAAGCT GACATGGTGG ATGGTATCAA GGAGAGTGTG 
TGCTGGGGCC CACCGCCCAC CACTAGCCCC CTCAAAAGCT GCGAGTATCA CGCCCTTTGC 
TCCCGTTTCC AGGAACTGCT GCTGCTTCCT GAGGACTATT TCATGCCTCC GCCGAAGCGT 
AGCCTGTGCT ACTGTGAGTC TTGCCGAAAA CTTCGAGGAG ATGAGGCCCA CAGGCGCCGA 
GGGGAGCCCC CCCGTGAATA TGCTCTGCCC TTTGGTTGGT GCAGGTTCAA CCTCAGGGTG 
AACCCCCGCC TGGAGGCTGG GACACTAACC AAGAAGTGGC ACATGGCCTA CCATGGAAGC 
AATGTGGCGG CTGTCCGGAG GGTGCTGGAC CGAGGGGAGC TGGGGGCAGG CACTGCTTCC 
ATCCTGAGCT GCCGGCCCTT GAAGGGAGAG CCTGGGACAG GGTTTGAGGA GCCTGGAGAG 
AACTGTGCGC CTCCCCGGGA GGAGCAGCCC CCTCCAGTGC TGCTTTCCCC CTCCCTCCAA 
TATGCTGGGG CTGAGACCCT GGCATCCAAA GTGCAATTCC GGGACCCCAA ATCCCAGCGG 
ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGTGTGCGCC CTGGCTCCTA CACCCCGGGA 
CCCCCTTCTG CGGCCCTCAG AGAGCCTCCC GATCCTCACT TCAGCCCAGC CGAACTTGAG 
TGGGTGACCA AGGAGAAAGG GGCCACGCTC CTCTATGCCC TGCTGGTACG GGTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEURL4
Entrez Gene ID	528485
Full Name	neuralized E3 ubiquitin protein ligase 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	NEURL4	XP_003315396.1
Mus musculus (house mouse)	Neurl4	NP_001013432.1
Canis lupus familiaris (dog)	NEURL4	XP_005620031.1
Danio rerio (zebrafish)	neurl4	XP_682914.5
Drosophila melanogaster (fruit fly)	blue	NP_648688.2
Homo sapiens (human)	NEURL4	NP_115818.2
	LOC714906	XP_002808159.1
Bos taurus (cattle)	NEURL4	NP_001179590.1
Rattus norvegicus (Norway rat)	Neurl4	NP_001100483.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011965	XP_320568.4
Xenopus tropicalis (tropical clawed frog)	neurl4	NP_001090721.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEURL4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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