UBR2 cDNA ORF clone, Bos taurus(cattle)

The following UBR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa32016 XM_005223448.4
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa51321 XM_010818202.2
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa229498 XM_024983620.1
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa39814 NM_001192638.1
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa32014 XM_005223446.4
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa32014 XM_010818199.3
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa32015 XM_005223447.4
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa51319 XM_010818200.3
Latest version!
Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OBa32016 XM_005223448.3 Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa32014 XM_005223446.3 Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa32014 XM_010818199.2 Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa32015 XM_005223447.3 Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa51319 XM_010818200.2 Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa32016
    Clone ID Related Accession (Same CDS sequence) XM_005223448.3 , XM_005223448.4
    Accession Version XM_005223448.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGTGTCTAAA TGTCTCAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223505.1
    CDS354..1655
    Translation

    Target ORF information:

    RefSeq Version XM_005223448.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223448.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGTGTCTAAA TGTCTCAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32016
    Clone ID Related Accession (Same CDS sequence) XM_005223448.3 , XM_005223448.4
    Accession Version XM_005223448.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223448.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGTGTCTAAA TGTCTCAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223505.1
    CDS359..1660
    Translation

    Target ORF information:

    RefSeq Version XM_005223448.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223448.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGTGTCTAAA TGTCTCAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51321
    Clone ID Related Accession (Same CDS sequence) XM_010818202.2
    Accession Version XM_010818202.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGGTG
    TCTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816504.1
    CDS348..2456
    Translation

    Target ORF information:

    RefSeq Version XM_010818202.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818202.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGGTG
    TCTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229498
    Clone ID Related Accession (Same CDS sequence) XM_024983620.1
    Accession Version XM_024983620.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGACAACAT CAGGAGGTGG AGGTTTCTGT GACTGTGGTG ATACTGAAGC TTGGAAAGAG 
    GGTCCTCACT GTCAGAAACA TGAACTTAAC ACCTATGAAA CTGAGGAAGA AGAGGATCCC
    CTTGTCCATT TATCAGAAGA TGTGATAGCA AGAACTTATA ACATTTTTGC TATTATGTTT
    CAATATGCGG TAGAGATACT AACCTGGGAA AAAGAAAGTG AATTGCCACC AGATTTAGAG
    ATGATTGAGA AGAGTGACAC CTACTATTGC ATGCTGTTTA ATGACGAGGT TCATACCTAT
    GAACAAGTTA TTTATACTCT TCAGAAAGCT GTTAACTGTA CACAAAAAGA AGCCATTGGC
    TTCGCAACTA CAGTAGATCG AGATGGACGT AGGTCTGTTC GATATGGAGA ATTCCAGTAT
    TGTGAACAAG CAAAATCAAT AATTGTGAGA AATACCAGTA GACAGACAAA GCCACTCAAA
    GTTCAAGTTA TGCATTCGTC TATTGTCGCA CATCAGAATT TTGGTTTGAA AGTGTTGTCT
    TGGCTGGGAA GTATTATTGG ATATTCAGAT GGCCTTCGCC GAATTTTATG TCAAGTTGGT
    TTACAAGAAG GGCCAGATGG TGAAAACTCT TCTCTAGTGG ATAGATTGAT GCTTAGTGAT
    TCCAAATTGT GGAAAGGTGC TAGGAGTGTG TATCATCAGT TGTTCATGAG CAGTCTACTT
    ATGGATTTGA AATACAAGAA ACTGTTTGCT GTTCGATTTG CAAAGAACTA TGAGCGTTTG
    CAGAGTGATT ATGTGACAGA TGACCACGAC AGAGAGTTTT CAATCGCAGA CTTCTCGGTT
    CAGATTTTCA CAGTCCCTTC ACTTGCTCGG ATGCTTATCA CAGAAGAAAA CCTGATGACC
    ATTATCATTA AGACTTTCAT GGATCATTTG AGACACCGAG ATGTCCAGGG CAGATTTCAG
    TTTGAACGAT ATACTGCTTT ACAAGCCTTT AAGTTTAGGA GGGTTCAGAG TCTTATTTTA
    GATCTCAAGT ATGTGTTAAT TAGCAAACCA ACTGAATGGT CAGATGGTCT GAGGCAGAAG
    TTCCTGGAAG GATTTGATGC CTTTTTGGAA TTACTCAAAT GTATGCAGGG AATGGATCCA
    ATTACACGTC AAGTAGGACA GCATATCGAA ATGGAACCAG AGTGGGAAGC AGCCTTCACA
    CTACAAATGA AATTAACACA TGTCATTTCG ATGATACAAG ACTGGTGTGC TTTAGACGAA
    AAAGTATTAA TTGAAGCTTA CAAGAAATGC CTTGCTGTAT TGACACAGTG TCATGGTGGT
    TTTACTGATG GTGAACAGCC AGTCACATTA AGCATTTGTG GACATTCAGT GGAAACTATC
    AGATACTGTG TTTCCAAAGA AAAAGTTAGC ATTCACCTCC CAGTTTCTCG CTTACTTGCA
    GGTTTGCATG TGTTGTTAAG CAAAAGTGAA GTGGCATATA AATTTCCAGA GCTCCTACCT
    CTAAGTGAAC TTAGCCCACC CATGTTGATA GAACACCCTC TTAGATGTCT TGTTTTATGT
    GCCCAAGTAC ATGCAGGAAT GTGGAGAAGA AATGGTTTCT CTCTCGTAAA CCAGATTTAT
    TACTACCACA ATGTGAAATG TAGACGTGAA ATGTTTGATA AGGATATAGT AATGCTTCAG
    ACAGGTGTCT CCATGATGGA TCCAAATCAT TTCCTGATGA TAATGCTTAG CCGCTTTGAA
    CTTTATCAGA TTTTCAGTAC CCCAGATTAT GGGAAGAAAA TTAGTTCTGA GACTCCTCAT
    AAGGATTTAG TTCAGCAGAA CAACACTCTA ATAGAAGAAA TGCTATACCT CATTATAATG
    CTTGTTGGAG AGAGATTTAG TCCTGGAGTT GGACAGGTTA ATGCTACAGA TGAGATTAAA
    CGAGAGATTA TCCATCAGTT GAGCATCAAA CCTATGGCTC ATAGTGAATT GGTAAAGTCT
    TTACCTGAAG ATGAGAACAA GGAGACTGGC ATGGAGAGTG TGATTGAAGC AGTTGCCCAT
    TTCAAGAAAC CTGGATTAAC AGGACGAGGC ATGTATGAAC TGAAACCAGA GTGTGCCAAA
    GAGTTCAATT TGTATTTCTA TCACTTTTCA AGGGCAGAGC AATCCAAGGC AGAGGAAGCT
    CAACGGAAAT TGAAAAAACA AAATAAAGAA GATACAGCAC TTCCGCCTCC AGTTTTGCCC
    CCATTCTGCC CTCTGTTTGC AAGTCTGGTT AACATTTTGC AGTCAGATGT CATGTTGTTA
    ATCATGAGGA CAGTGTTGCA GTGGACTGTA GAACATAGTG GACATATCTG GTCTGAGTCC
    ATGCTGCAAA GGGTATTACA TTTAATTGGA ATGGCACTAC AAGAAGAAAA ACAGCATTTA
    GAGAATGTCA TGGAGGAGCA TGTCATAACA TTTACCTTCA CACAGAAGAT ATCGAAACCT
    GGTGAGGCTC CAAACAACTC ACCTAGTATA CTAGCTATGC TGGAAACACT GCAGAATGCT
    CCCTACCTAG AAGTCCACAA AGATATGATT AAGTGGATAT TAAAGACTTT TAATGCTATT
    AAGAAGTTAA GGGAGAGTTC ATCCACCAGT CCTGTGGCAG AGACAGAAGG AACCATAATG
    GAAGAGAGTT CGAGAGACAA AGACAAAGCT GAGAGGAAGA GAAAAGCTGA GATTGCCAGA
    TTACGCAGAG AGAAGATCAT GGCTCAGATG TCTGAGATGC AGCGGCACTT TATTGATGAG
    AACAAAGAAC TCTTTCAGCA GACGTTAGAA CTGGATTCCT CTGTCTCTGC TGTACTTGAT
    AATGGCCCTG TGGTTTCAGA TATGACTCTT ACAGCATTGG GCCCAGCACA GACTCGGGTC
    CCTGAACAGA GACAGTGTGT CACCTGTATA TTGTGTCAGG AAGAGCAAGA GGTGAATGCA
    GAAAGCAAGG CAATGGTCCT GGCAGCATTT GTGCAGAGAT CAACTGTGTT ATCAAAAGAC
    AGAAGTAAAT TCATTCAGGA TCCAGAAAAT TATGATCCAT TATTCATGCA CCCTGATCTG
    TCTTGTGGAA TACATACTGG TAGCTGTGGG CATATTATGC ATGCACACTG TTGGCAAAGG
    TATTTTGATT CCGTTCAAGC TAAAGAACAG CGAAGGCAAC AGAGATTACG CTTACATACA
    AGCTATGATG TAGAAAATGG AGAATTCCTT TGTCCCCTTT GTGAATGCTT GAGTAATACT
    GTTATTCCTC TGCTGCTTCC TCCAAGAAAT GTTTTTCATG GCAGGTTAAA TTTTTCAGAC
    CAACCAAATC TGACTCAGTG GATTAGAACA ATATCTCAGC AAATAAAAGC ATTACAGGTT
    CTTAGGAAAG AAGAAAGTAC TCCTATTACT GCCTCATCGA AGAATTTAGA AAATATGGAT
    GAATTGCACC TTCCTGAAGG ATTTAGACCT GATTTTCATC CTAAGAACCC TTATTCTGAA
    AGCATAAAAG AAATGTTAGC AACGTTTGGA ACTGCTACTT ATAAGGTGGG ATTAAAAGTT
    CATCCCAATG AAGAGGATCC CCGTGTGCCC ATAATGTGTT GGAGTAGTTG TGCATATACC
    ATCCAAAGCA TAGAAAGAAT TTTAAGTGAT GAAGATAAAC CATTGTTTGG TCCTTTGCCT
    TGTAGACTGG ATGACTGTCT CAGGTCACTA ACAAGATTTG CTGGAGCACA TTGGACAGTG
    GTGTCACTTT CAGTGGTGCA AGGACACTTT TGTAAACTTT TTGCATCATT GGTGCCTGGT
    GACAACCACA GAGACCTTCC ATGCATATTA GATATCGACA TGTTTCATTT ATTGGTGGGC
    TTGGTCCTTG CCTTCCCTTC ACTGCAGTGT CAGGACTTTT CAGGGATCAG CCTGGGCACT
    GGGGACCTTC ACATTTTCCA TCTGGTTACC ATGGCACACA TCGTTCAGAT CTTACTTACC
    TCGTGTACAG AAAAGAGTGG CATGGATCAA GAAAATGCTG CTGGCAAAGA AGAAGAACTA
    GCAGTTCTTG CTTTGTGTAA AATGATTCAG CAGTATACAG GAAGTGCCTT GAAAGAAATG
    CCATCTGGTT GGCATCTGTG GAGGAATGTC AAAGCTGGAA TCATGCCTTT TCTGAGGTGT
    TCTGCTTTGT TTTTCCATTA CTTAAATGGA GTTCCTTCCC CACCTGAAAT TCAAGCTTCT
    GGAACAAGCC ATTTTGAACA TCTATGTAAC TATCTTTCCC TGCCAAACAA CCTTCTTTGT
    CTTTTTCAAG AAAATAAGGA GATAATGAAA TTACTGATTG AAAGTTGGTG TCATAACATC
    GAAGTTAAAA GATACCTGGA AGGTGAAAGA GACGCTATAA GTTATCCAAG AGAATCTAAC
    AAATTAATAG ACCTTCCAGA GGATTACAGC AGCCTCATTA ATCAAGCATC TAATTTCTCG
    TGCCCCAAAT CAGGTGGTGA TAAGAGCAGA GCCCCAACTA TGTGCCTTGT ATGCGGAACC
    CTGCTGTGCT CCCAGAGCTA CTGCTGCCAG ACGGAGTTGG AAGGGGAGGA CGTGGGGGCA
    TGCACTGCTC ACACCTATTC CTGTGGTTCT GGGGTGGGCA TCTTCCTGAG AGTACGAGAA
    TGTCAAGTAC TATTTTTAGC TGGCAAAACC AAAGGCTGTT TTTATTCTCC TCCTTACCTT
    GATGACTATG GGGAGACTGA CCAGGGACTC AGACGGGGAA ATCCTTTACA TTTGTGCAGA
    GAGCGATTCA AGAAGATTCA GAAGCTCTGG CACCAGCACA GTATCACAGA GGAAATTGGA
    CACGCACAGG AAGCAAACCA GACATTGGTT GGCATTGACT GGCAACACCT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839388.1
    CDS114..4967
    Translation

    Target ORF information:

    RefSeq Version XM_024983620.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGACAACAT CAGGAGGTGG AGGTTTCTGT GACTGTGGTG ATACTGAAGC TTGGAAAGAG 
    GGTCCTCACT GTCAGAAACA TGAACTTAAC ACCTATGAAA CTGAGGAAGA AGAGGATCCC
    CTTGTCCATT TATCAGAAGA TGTGATAGCA AGAACTTATA ACATTTTTGC TATTATGTTT
    CAATATGCGG TAGAGATACT AACCTGGGAA AAAGAAAGTG AATTGCCACC AGATTTAGAG
    ATGATTGAGA AGAGTGACAC CTACTATTGC ATGCTGTTTA ATGACGAGGT TCATACCTAT
    GAACAAGTTA TTTATACTCT TCAGAAAGCT GTTAACTGTA CACAAAAAGA AGCCATTGGC
    TTCGCAACTA CAGTAGATCG AGATGGACGT AGGTCTGTTC GATATGGAGA ATTCCAGTAT
    TGTGAACAAG CAAAATCAAT AATTGTGAGA AATACCAGTA GACAGACAAA GCCACTCAAA
    GTTCAAGTTA TGCATTCGTC TATTGTCGCA CATCAGAATT TTGGTTTGAA AGTGTTGTCT
    TGGCTGGGAA GTATTATTGG ATATTCAGAT GGCCTTCGCC GAATTTTATG TCAAGTTGGT
    TTACAAGAAG GGCCAGATGG TGAAAACTCT TCTCTAGTGG ATAGATTGAT GCTTAGTGAT
    TCCAAATTGT GGAAAGGTGC TAGGAGTGTG TATCATCAGT TGTTCATGAG CAGTCTACTT
    ATGGATTTGA AATACAAGAA ACTGTTTGCT GTTCGATTTG CAAAGAACTA TGAGCGTTTG
    CAGAGTGATT ATGTGACAGA TGACCACGAC AGAGAGTTTT CAATCGCAGA CTTCTCGGTT
    CAGATTTTCA CAGTCCCTTC ACTTGCTCGG ATGCTTATCA CAGAAGAAAA CCTGATGACC
    ATTATCATTA AGACTTTCAT GGATCATTTG AGACACCGAG ATGTCCAGGG CAGATTTCAG
    TTTGAACGAT ATACTGCTTT ACAAGCCTTT AAGTTTAGGA GGGTTCAGAG TCTTATTTTA
    GATCTCAAGT ATGTGTTAAT TAGCAAACCA ACTGAATGGT CAGATGGTCT GAGGCAGAAG
    TTCCTGGAAG GATTTGATGC CTTTTTGGAA TTACTCAAAT GTATGCAGGG AATGGATCCA
    ATTACACGTC AAGTAGGACA GCATATCGAA ATGGAACCAG AGTGGGAAGC AGCCTTCACA
    CTACAAATGA AATTAACACA TGTCATTTCG ATGATACAAG ACTGGTGTGC TTTAGACGAA
    AAAGTATTAA TTGAAGCTTA CAAGAAATGC CTTGCTGTAT TGACACAGTG TCATGGTGGT
    TTTACTGATG GTGAACAGCC AGTCACATTA AGCATTTGTG GACATTCAGT GGAAACTATC
    AGATACTGTG TTTCCAAAGA AAAAGTTAGC ATTCACCTCC CAGTTTCTCG CTTACTTGCA
    GGTTTGCATG TGTTGTTAAG CAAAAGTGAA GTGGCATATA AATTTCCAGA GCTCCTACCT
    CTAAGTGAAC TTAGCCCACC CATGTTGATA GAACACCCTC TTAGATGTCT TGTTTTATGT
    GCCCAAGTAC ATGCAGGAAT GTGGAGAAGA AATGGTTTCT CTCTCGTAAA CCAGATTTAT
    TACTACCACA ATGTGAAATG TAGACGTGAA ATGTTTGATA AGGATATAGT AATGCTTCAG
    ACAGGTGTCT CCATGATGGA TCCAAATCAT TTCCTGATGA TAATGCTTAG CCGCTTTGAA
    CTTTATCAGA TTTTCAGTAC CCCAGATTAT GGGAAGAAAA TTAGTTCTGA GACTCCTCAT
    AAGGATTTAG TTCAGCAGAA CAACACTCTA ATAGAAGAAA TGCTATACCT CATTATAATG
    CTTGTTGGAG AGAGATTTAG TCCTGGAGTT GGACAGGTTA ATGCTACAGA TGAGATTAAA
    CGAGAGATTA TCCATCAGTT GAGCATCAAA CCTATGGCTC ATAGTGAATT GGTAAAGTCT
    TTACCTGAAG ATGAGAACAA GGAGACTGGC ATGGAGAGTG TGATTGAAGC AGTTGCCCAT
    TTCAAGAAAC CTGGATTAAC AGGACGAGGC ATGTATGAAC TGAAACCAGA GTGTGCCAAA
    GAGTTCAATT TGTATTTCTA TCACTTTTCA AGGGCAGAGC AATCCAAGGC AGAGGAAGCT
    CAACGGAAAT TGAAAAAACA AAATAAAGAA GATACAGCAC TTCCGCCTCC AGTTTTGCCC
    CCATTCTGCC CTCTGTTTGC AAGTCTGGTT AACATTTTGC AGTCAGATGT CATGTTGTTA
    ATCATGAGGA CAGTGTTGCA GTGGACTGTA GAACATAGTG GACATATCTG GTCTGAGTCC
    ATGCTGCAAA GGGTATTACA TTTAATTGGA ATGGCACTAC AAGAAGAAAA ACAGCATTTA
    GAGAATGTCA TGGAGGAGCA TGTCATAACA TTTACCTTCA CACAGAAGAT ATCGAAACCT
    GGTGAGGCTC CAAACAACTC ACCTAGTATA CTAGCTATGC TGGAAACACT GCAGAATGCT
    CCCTACCTAG AAGTCCACAA AGATATGATT AAGTGGATAT TAAAGACTTT TAATGCTATT
    AAGAAGTTAA GGGAGAGTTC ATCCACCAGT CCTGTGGCAG AGACAGAAGG AACCATAATG
    GAAGAGAGTT CGAGAGACAA AGACAAAGCT GAGAGGAAGA GAAAAGCTGA GATTGCCAGA
    TTACGCAGAG AGAAGATCAT GGCTCAGATG TCTGAGATGC AGCGGCACTT TATTGATGAG
    AACAAAGAAC TCTTTCAGCA GACGTTAGAA CTGGATTCCT CTGTCTCTGC TGTACTTGAT
    AATGGCCCTG TGGTTTCAGA TATGACTCTT ACAGCATTGG GCCCAGCACA GACTCGGGTC
    CCTGAACAGA GACAGTGTGT CACCTGTATA TTGTGTCAGG AAGAGCAAGA GGTGAATGCA
    GAAAGCAAGG CAATGGTCCT GGCAGCATTT GTGCAGAGAT CAACTGTGTT ATCAAAAGAC
    AGAAGTAAAT TCATTCAGGA TCCAGAAAAT TATGATCCAT TATTCATGCA CCCTGATCTG
    TCTTGTGGAA TACATACTGG TAGCTGTGGG CATATTATGC ATGCACACTG TTGGCAAAGG
    TATTTTGATT CCGTTCAAGC TAAAGAACAG CGAAGGCAAC AGAGATTACG CTTACATACA
    AGCTATGATG TAGAAAATGG AGAATTCCTT TGTCCCCTTT GTGAATGCTT GAGTAATACT
    GTTATTCCTC TGCTGCTTCC TCCAAGAAAT GTTTTTCATG GCAGGTTAAA TTTTTCAGAC
    CAACCAAATC TGACTCAGTG GATTAGAACA ATATCTCAGC AAATAAAAGC ATTACAGGTT
    CTTAGGAAAG AAGAAAGTAC TCCTATTACT GCCTCATCGA AGAATTTAGA AAATATGGAT
    GAATTGCACC TTCCTGAAGG ATTTAGACCT GATTTTCATC CTAAGAACCC TTATTCTGAA
    AGCATAAAAG AAATGTTAGC AACGTTTGGA ACTGCTACTT ATAAGGTGGG ATTAAAAGTT
    CATCCCAATG AAGAGGATCC CCGTGTGCCC ATAATGTGTT GGAGTAGTTG TGCATATACC
    ATCCAAAGCA TAGAAAGAAT TTTAAGTGAT GAAGATAAAC CATTGTTTGG TCCTTTGCCT
    TGTAGACTGG ATGACTGTCT CAGGTCACTA ACAAGATTTG CTGGAGCACA TTGGACAGTG
    GTGTCACTTT CAGTGGTGCA AGGACACTTT TGTAAACTTT TTGCATCATT GGTGCCTGGT
    GACAACCACA GAGACCTTCC ATGCATATTA GATATCGACA TGTTTCATTT ATTGGTGGGC
    TTGGTCCTTG CCTTCCCTTC ACTGCAGTGT CAGGACTTTT CAGGGATCAG CCTGGGCACT
    GGGGACCTTC ACATTTTCCA TCTGGTTACC ATGGCACACA TCGTTCAGAT CTTACTTACC
    TCGTGTACAG AAAAGAGTGG CATGGATCAA GAAAATGCTG CTGGCAAAGA AGAAGAACTA
    GCAGTTCTTG CTTTGTGTAA AATGATTCAG CAGTATACAG GAAGTGCCTT GAAAGAAATG
    CCATCTGGTT GGCATCTGTG GAGGAATGTC AAAGCTGGAA TCATGCCTTT TCTGAGGTGT
    TCTGCTTTGT TTTTCCATTA CTTAAATGGA GTTCCTTCCC CACCTGAAAT TCAAGCTTCT
    GGAACAAGCC ATTTTGAACA TCTATGTAAC TATCTTTCCC TGCCAAACAA CCTTCTTTGT
    CTTTTTCAAG AAAATAAGGA GATAATGAAA TTACTGATTG AAAGTTGGTG TCATAACATC
    GAAGTTAAAA GATACCTGGA AGGTGAAAGA GACGCTATAA GTTATCCAAG AGAATCTAAC
    AAATTAATAG ACCTTCCAGA GGATTACAGC AGCCTCATTA ATCAAGCATC TAATTTCTCG
    TGCCCCAAAT CAGGTGGTGA TAAGAGCAGA GCCCCAACTA TGTGCCTTGT ATGCGGAACC
    CTGCTGTGCT CCCAGAGCTA CTGCTGCCAG ACGGAGTTGG AAGGGGAGGA CGTGGGGGCA
    TGCACTGCTC ACACCTATTC CTGTGGTTCT GGGGTGGGCA TCTTCCTGAG AGTACGAGAA
    TGTCAAGTAC TATTTTTAGC TGGCAAAACC AAAGGCTGTT TTTATTCTCC TCCTTACCTT
    GATGACTATG GGGAGACTGA CCAGGGACTC AGACGGGGAA ATCCTTTACA TTTGTGCAGA
    GAGCGATTCA AGAAGATTCA GAAGCTCTGG CACCAGCACA GTATCACAGA GGAAATTGGA
    CACGCACAGG AAGCAAACCA GACATTGGTT GGCATTGACT GGCAACACCT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39814
    Clone ID Related Accession (Same CDS sequence) NM_001192638.1
    Accession Version NM_001192638.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000023.1. On Jul 17, 2010 this sequence version replaced XM_605543.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.48416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAATTACCA GCAGTTGCAG
    AGAGATTTTA TGGAGGATGA TCACGAGCGA GCAGTGTCCG TGACTGCTCT GTCTGTCCAG
    TTCTTCACCG CACCTACTCT GGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179567.1
    CDS1..5271
    Translation

    Target ORF information:

    RefSeq Version NM_001192638.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAATTACCA GCAGTTGCAG
    AGAGATTTTA TGGAGGATGA TCACGAGCGA GCAGTGTCCG TGACTGCTCT GTCTGTCCAG
    TTCTTCACCG CACCTACTCT GGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32014
    Clone ID Related Accession (Same CDS sequence) XM_005223446.4 , XM_005223446.3 , XM_010818199.3 , XM_010818199.2
    Accession Version XM_005223446.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223446.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223503.1
    CDS345..5615
    Translation

    Target ORF information:

    RefSeq Version XM_005223446.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223446.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32014
    Clone ID Related Accession (Same CDS sequence) XM_005223446.4 , XM_005223446.3 , XM_010818199.3 , XM_010818199.2
    Accession Version XM_005223446.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223503.1
    CDS330..5600
    Translation

    Target ORF information:

    RefSeq Version XM_005223446.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223446.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32014
    Clone ID Related Accession (Same CDS sequence) XM_005223446.4 , XM_005223446.3 , XM_010818199.3 , XM_010818199.2
    Accession Version XM_010818199.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816501.1
    CDS279..5549
    Translation

    Target ORF information:

    RefSeq Version XM_010818199.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818199.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32014
    Clone ID Related Accession (Same CDS sequence) XM_005223446.4 , XM_005223446.3 , XM_010818199.3 , XM_010818199.2
    Accession Version XM_010818199.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816501.1
    CDS233..5503
    Translation

    Target ORF information:

    RefSeq Version XM_010818199.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818199.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTGGTGTCA TAACATCGAA
    GTTAAAAGAT ACCTGGAAGG TGAAAGAGAC GCTATAAGTT ATCCAAGAGA ATCTAACAAA
    TTAATAGACC TTCCAGAGGA TTACAGCAGC CTCATTAATC AAGCATCTAA TTTCTCGTGC
    CCCAAATCAG GTGGTGATAA GAGCAGAGCC CCAACTATGT GCCTTGTATG CGGAACCCTG
    CTGTGCTCCC AGAGCTACTG CTGCCAGACG GAGTTGGAAG GGGAGGACGT GGGGGCATGC
    ACTGCTCACA CCTATTCCTG TGGTTCTGGG GTGGGCATCT TCCTGAGAGT ACGAGAATGT
    CAAGTACTAT TTTTAGCTGG CAAAACCAAA GGCTGTTTTT ATTCTCCTCC TTACCTTGAT
    GACTATGGGG AGACTGACCA GGGACTCAGA CGGGGAAATC CTTTACATTT GTGCAGAGAG
    CGATTCAAGA AGATTCAGAA GCTCTGGCAC CAGCACAGTA TCACAGAGGA AATTGGACAC
    GCACAGGAAG CAAACCAGAC ATTGGTTGGC ATTGACTGGC AACACCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32015
    Clone ID Related Accession (Same CDS sequence) XM_005223447.4 , XM_005223447.3
    Accession Version XM_005223447.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223447.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGGT TAAATTTTTC AGACCAACCA
    AATCTGACTC AGTGGATTAG AACAATATCT CAGCAAATAA AAGCATTACA GGTTCTTAGG
    AAAGAAGAAA GTACTCCTAT TACTGCCTCA TCGAAGAATT TAGAAAATAT GGATGAATTG
    CACCTTCCTG AAGGATTTAG ACCTGATTTT CATCCTAAGA ACCCTTATTC TGAAAGCATA
    AAAGAAATGT TAGCAACGTT TGGAACTGCT ACTTATAAGG TGGGATTAAA AGTTCATCCC
    AATGAAGAGG ATCCCCGTGT GCCCATAATG TGTTGGAGTA GTTGTGCATA TACCATCCAA
    AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTGT TTGGTCCTTT GCCTTGTAGA
    CTGGATGACT GTCTCAGGTC ACTAACAAGA TTTGCTGGAG CACATTGGAC AGTGGTGTCA
    CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC TGGTGACAAC
    CACAGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTC
    CTTGCCTTCC CTTCACTGCA GTGTCAGGAC TTTTCAGGGA TCAGCCTGGG CACTGGGGAC
    CTTCACATTT TCCATCTGGT TACCATGGCA CACATCGTTC AGATCTTACT TACCTCGTGT
    ACAGAAAAGA GTGGCATGGA TCAAGAAAAT GCTGCTGGCA AAGAAGAAGA ACTAGCAGTT
    CTTGCTTTGT GTAAAATGAT TCAGCAGTAT ACAGGAAGTG CCTTGAAAGA AATGCCATCT
    GGTTGGCATC TGTGGAGGAA TGTCAAAGCT GGAATCATGC CTTTTCTGAG GTGTTCTGCT
    TTGTTTTTCC ATTACTTAAA TGGAGTTCCT TCCCCACCTG AAATTCAAGC TTCTGGAACA
    AGCCATTTTG AACATCTATG TAACTATCTT TCCCTGCCAA ACAACCTTCT TTGTCTTTTT
    CAAGAAAATA AGGAGATAAT GAAATTACTG ATTGAAAGTT GGTGTCATAA CATCGAAGTT
    AAAAGATACC TGGAAGGTGA AAGAGACGCT ATAAGTTATC CAAGAGAATC TAACAAATTA
    ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAATCAAG CATCTAATTT CTCGTGCCCC
    AAATCAGGTG GTGATAAGAG CAGAGCCCCA ACTATGTGCC TTGTATGCGG AACCCTGCTG
    TGCTCCCAGA GCTACTGCTG CCAGACGGAG TTGGAAGGGG AGGACGTGGG GGCATGCACT
    GCTCACACCT ATTCCTGTGG TTCTGGGGTG GGCATCTTCC TGAGAGTACG AGAATGTCAA
    GTACTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC
    TATGGGGAGA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAGAGAGCGA
    TTCAAGAAGA TTCAGAAGCT CTGGCACCAG CACAGTATCA CAGAGGAAAT TGGACACGCA
    CAGGAAGCAA ACCAGACATT GGTTGGCATT GACTGGCAAC ACCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223504.1
    CDS345..5612
    Translation

    Target ORF information:

    RefSeq Version XM_005223447.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223447.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGGT TAAATTTTTC AGACCAACCA
    AATCTGACTC AGTGGATTAG AACAATATCT CAGCAAATAA AAGCATTACA GGTTCTTAGG
    AAAGAAGAAA GTACTCCTAT TACTGCCTCA TCGAAGAATT TAGAAAATAT GGATGAATTG
    CACCTTCCTG AAGGATTTAG ACCTGATTTT CATCCTAAGA ACCCTTATTC TGAAAGCATA
    AAAGAAATGT TAGCAACGTT TGGAACTGCT ACTTATAAGG TGGGATTAAA AGTTCATCCC
    AATGAAGAGG ATCCCCGTGT GCCCATAATG TGTTGGAGTA GTTGTGCATA TACCATCCAA
    AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTGT TTGGTCCTTT GCCTTGTAGA
    CTGGATGACT GTCTCAGGTC ACTAACAAGA TTTGCTGGAG CACATTGGAC AGTGGTGTCA
    CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC TGGTGACAAC
    CACAGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTC
    CTTGCCTTCC CTTCACTGCA GTGTCAGGAC TTTTCAGGGA TCAGCCTGGG CACTGGGGAC
    CTTCACATTT TCCATCTGGT TACCATGGCA CACATCGTTC AGATCTTACT TACCTCGTGT
    ACAGAAAAGA GTGGCATGGA TCAAGAAAAT GCTGCTGGCA AAGAAGAAGA ACTAGCAGTT
    CTTGCTTTGT GTAAAATGAT TCAGCAGTAT ACAGGAAGTG CCTTGAAAGA AATGCCATCT
    GGTTGGCATC TGTGGAGGAA TGTCAAAGCT GGAATCATGC CTTTTCTGAG GTGTTCTGCT
    TTGTTTTTCC ATTACTTAAA TGGAGTTCCT TCCCCACCTG AAATTCAAGC TTCTGGAACA
    AGCCATTTTG AACATCTATG TAACTATCTT TCCCTGCCAA ACAACCTTCT TTGTCTTTTT
    CAAGAAAATA AGGAGATAAT GAAATTACTG ATTGAAAGTT GGTGTCATAA CATCGAAGTT
    AAAAGATACC TGGAAGGTGA AAGAGACGCT ATAAGTTATC CAAGAGAATC TAACAAATTA
    ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAATCAAG CATCTAATTT CTCGTGCCCC
    AAATCAGGTG GTGATAAGAG CAGAGCCCCA ACTATGTGCC TTGTATGCGG AACCCTGCTG
    TGCTCCCAGA GCTACTGCTG CCAGACGGAG TTGGAAGGGG AGGACGTGGG GGCATGCACT
    GCTCACACCT ATTCCTGTGG TTCTGGGGTG GGCATCTTCC TGAGAGTACG AGAATGTCAA
    GTACTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC
    TATGGGGAGA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAGAGAGCGA
    TTCAAGAAGA TTCAGAAGCT CTGGCACCAG CACAGTATCA CAGAGGAAAT TGGACACGCA
    CAGGAAGCAA ACCAGACATT GGTTGGCATT GACTGGCAAC ACCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32015
    Clone ID Related Accession (Same CDS sequence) XM_005223447.4 , XM_005223447.3
    Accession Version XM_005223447.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGGT TAAATTTTTC AGACCAACCA
    AATCTGACTC AGTGGATTAG AACAATATCT CAGCAAATAA AAGCATTACA GGTTCTTAGG
    AAAGAAGAAA GTACTCCTAT TACTGCCTCA TCGAAGAATT TAGAAAATAT GGATGAATTG
    CACCTTCCTG AAGGATTTAG ACCTGATTTT CATCCTAAGA ACCCTTATTC TGAAAGCATA
    AAAGAAATGT TAGCAACGTT TGGAACTGCT ACTTATAAGG TGGGATTAAA AGTTCATCCC
    AATGAAGAGG ATCCCCGTGT GCCCATAATG TGTTGGAGTA GTTGTGCATA TACCATCCAA
    AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTGT TTGGTCCTTT GCCTTGTAGA
    CTGGATGACT GTCTCAGGTC ACTAACAAGA TTTGCTGGAG CACATTGGAC AGTGGTGTCA
    CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC TGGTGACAAC
    CACAGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTC
    CTTGCCTTCC CTTCACTGCA GTGTCAGGAC TTTTCAGGGA TCAGCCTGGG CACTGGGGAC
    CTTCACATTT TCCATCTGGT TACCATGGCA CACATCGTTC AGATCTTACT TACCTCGTGT
    ACAGAAAAGA GTGGCATGGA TCAAGAAAAT GCTGCTGGCA AAGAAGAAGA ACTAGCAGTT
    CTTGCTTTGT GTAAAATGAT TCAGCAGTAT ACAGGAAGTG CCTTGAAAGA AATGCCATCT
    GGTTGGCATC TGTGGAGGAA TGTCAAAGCT GGAATCATGC CTTTTCTGAG GTGTTCTGCT
    TTGTTTTTCC ATTACTTAAA TGGAGTTCCT TCCCCACCTG AAATTCAAGC TTCTGGAACA
    AGCCATTTTG AACATCTATG TAACTATCTT TCCCTGCCAA ACAACCTTCT TTGTCTTTTT
    CAAGAAAATA AGGAGATAAT GAAATTACTG ATTGAAAGTT GGTGTCATAA CATCGAAGTT
    AAAAGATACC TGGAAGGTGA AAGAGACGCT ATAAGTTATC CAAGAGAATC TAACAAATTA
    ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAATCAAG CATCTAATTT CTCGTGCCCC
    AAATCAGGTG GTGATAAGAG CAGAGCCCCA ACTATGTGCC TTGTATGCGG AACCCTGCTG
    TGCTCCCAGA GCTACTGCTG CCAGACGGAG TTGGAAGGGG AGGACGTGGG GGCATGCACT
    GCTCACACCT ATTCCTGTGG TTCTGGGGTG GGCATCTTCC TGAGAGTACG AGAATGTCAA
    GTACTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC
    TATGGGGAGA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAGAGAGCGA
    TTCAAGAAGA TTCAGAAGCT CTGGCACCAG CACAGTATCA CAGAGGAAAT TGGACACGCA
    CAGGAAGCAA ACCAGACATT GGTTGGCATT GACTGGCAAC ACCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223504.1
    CDS330..5597
    Translation

    Target ORF information:

    RefSeq Version XM_005223447.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223447.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGGT TAAATTTTTC AGACCAACCA
    AATCTGACTC AGTGGATTAG AACAATATCT CAGCAAATAA AAGCATTACA GGTTCTTAGG
    AAAGAAGAAA GTACTCCTAT TACTGCCTCA TCGAAGAATT TAGAAAATAT GGATGAATTG
    CACCTTCCTG AAGGATTTAG ACCTGATTTT CATCCTAAGA ACCCTTATTC TGAAAGCATA
    AAAGAAATGT TAGCAACGTT TGGAACTGCT ACTTATAAGG TGGGATTAAA AGTTCATCCC
    AATGAAGAGG ATCCCCGTGT GCCCATAATG TGTTGGAGTA GTTGTGCATA TACCATCCAA
    AGCATAGAAA GAATTTTAAG TGATGAAGAT AAACCATTGT TTGGTCCTTT GCCTTGTAGA
    CTGGATGACT GTCTCAGGTC ACTAACAAGA TTTGCTGGAG CACATTGGAC AGTGGTGTCA
    CTTTCAGTGG TGCAAGGACA CTTTTGTAAA CTTTTTGCAT CATTGGTGCC TGGTGACAAC
    CACAGAGACC TTCCATGCAT ATTAGATATC GACATGTTTC ATTTATTGGT GGGCTTGGTC
    CTTGCCTTCC CTTCACTGCA GTGTCAGGAC TTTTCAGGGA TCAGCCTGGG CACTGGGGAC
    CTTCACATTT TCCATCTGGT TACCATGGCA CACATCGTTC AGATCTTACT TACCTCGTGT
    ACAGAAAAGA GTGGCATGGA TCAAGAAAAT GCTGCTGGCA AAGAAGAAGA ACTAGCAGTT
    CTTGCTTTGT GTAAAATGAT TCAGCAGTAT ACAGGAAGTG CCTTGAAAGA AATGCCATCT
    GGTTGGCATC TGTGGAGGAA TGTCAAAGCT GGAATCATGC CTTTTCTGAG GTGTTCTGCT
    TTGTTTTTCC ATTACTTAAA TGGAGTTCCT TCCCCACCTG AAATTCAAGC TTCTGGAACA
    AGCCATTTTG AACATCTATG TAACTATCTT TCCCTGCCAA ACAACCTTCT TTGTCTTTTT
    CAAGAAAATA AGGAGATAAT GAAATTACTG ATTGAAAGTT GGTGTCATAA CATCGAAGTT
    AAAAGATACC TGGAAGGTGA AAGAGACGCT ATAAGTTATC CAAGAGAATC TAACAAATTA
    ATAGACCTTC CAGAGGATTA CAGCAGCCTC ATTAATCAAG CATCTAATTT CTCGTGCCCC
    AAATCAGGTG GTGATAAGAG CAGAGCCCCA ACTATGTGCC TTGTATGCGG AACCCTGCTG
    TGCTCCCAGA GCTACTGCTG CCAGACGGAG TTGGAAGGGG AGGACGTGGG GGCATGCACT
    GCTCACACCT ATTCCTGTGG TTCTGGGGTG GGCATCTTCC TGAGAGTACG AGAATGTCAA
    GTACTATTTT TAGCTGGCAA AACCAAAGGC TGTTTTTATT CTCCTCCTTA CCTTGATGAC
    TATGGGGAGA CTGACCAGGG ACTCAGACGG GGAAATCCTT TACATTTGTG CAGAGAGCGA
    TTCAAGAAGA TTCAGAAGCT CTGGCACCAG CACAGTATCA CAGAGGAAAT TGGACACGCA
    CAGGAAGCAA ACCAGACATT GGTTGGCATT GACTGGCAAC ACCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51319
    Clone ID Related Accession (Same CDS sequence) XM_010818200.2 , XM_010818200.3
    Accession Version XM_010818200.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTATCCAAG AGAATCTAAC
    AAATTAATAG ACCTTCCAGA GGATTACAGC AGCCTCATTA ATCAAGCATC TAATTTCTCG
    TGCCCCAAAT CAGGTGGTGA TAAGAGCAGA GCCCCAACTA TGTGCCTTGT ATGCGGAACC
    CTGCTGTGCT CCCAGAGCTA CTGCTGCCAG ACGGAGTTGG AAGGGGAGGA CGTGGGGGCA
    TGCACTGCTC ACACCTATTC CTGTGGTTCT GGGGTGGGCA TCTTCCTGAG AGTACGAGAA
    TGTCAAGTAC TATTTTTAGC TGGCAAAACC AAAGGCTGTT TTTATTCTCC TCCTTACCTT
    GATGACTATG GGGAGACTGA CCAGGGACTC AGACGGGGAA ATCCTTTACA TTTGTGCAGA
    GAGCGATTCA AGAAGATTCA GAAGCTCTGG CACCAGCACA GTATCACAGA GGAAATTGGA
    CACGCACAGG AAGCAAACCA GACATTGGTT GGCATTGACT GGCAACACCT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816502.1
    CDS331..5544
    Translation

    Target ORF information:

    RefSeq Version XM_010818200.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTATCCAAG AGAATCTAAC
    AAATTAATAG ACCTTCCAGA GGATTACAGC AGCCTCATTA ATCAAGCATC TAATTTCTCG
    TGCCCCAAAT CAGGTGGTGA TAAGAGCAGA GCCCCAACTA TGTGCCTTGT ATGCGGAACC
    CTGCTGTGCT CCCAGAGCTA CTGCTGCCAG ACGGAGTTGG AAGGGGAGGA CGTGGGGGCA
    TGCACTGCTC ACACCTATTC CTGTGGTTCT GGGGTGGGCA TCTTCCTGAG AGTACGAGAA
    TGTCAAGTAC TATTTTTAGC TGGCAAAACC AAAGGCTGTT TTTATTCTCC TCCTTACCTT
    GATGACTATG GGGAGACTGA CCAGGGACTC AGACGGGGAA ATCCTTTACA TTTGTGCAGA
    GAGCGATTCA AGAAGATTCA GAAGCTCTGG CACCAGCACA GTATCACAGA GGAAATTGGA
    CACGCACAGG AAGCAAACCA GACATTGGTT GGCATTGACT GGCAACACCT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51319
    Clone ID Related Accession (Same CDS sequence) XM_010818200.2 , XM_010818200.3
    Accession Version XM_010818200.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase UBR2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTATCCAAG AGAATCTAAC
    AAATTAATAG ACCTTCCAGA GGATTACAGC AGCCTCATTA ATCAAGCATC TAATTTCTCG
    TGCCCCAAAT CAGGTGGTGA TAAGAGCAGA GCCCCAACTA TGTGCCTTGT ATGCGGAACC
    CTGCTGTGCT CCCAGAGCTA CTGCTGCCAG ACGGAGTTGG AAGGGGAGGA CGTGGGGGCA
    TGCACTGCTC ACACCTATTC CTGTGGTTCT GGGGTGGGCA TCTTCCTGAG AGTACGAGAA
    TGTCAAGTAC TATTTTTAGC TGGCAAAACC AAAGGCTGTT TTTATTCTCC TCCTTACCTT
    GATGACTATG GGGAGACTGA CCAGGGACTC AGACGGGGAA ATCCTTTACA TTTGTGCAGA
    GAGCGATTCA AGAAGATTCA GAAGCTCTGG CACCAGCACA GTATCACAGA GGAAATTGGA
    CACGCACAGG AAGCAAACCA GACATTGGTT GGCATTGACT GGCAACACCT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816502.1
    CDS346..5559
    Translation

    Target ORF information:

    RefSeq Version XM_010818200.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818200.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGTCGG AGCTGGAGCC TGAGGTGCAG GCCCTCGACC GGAGTTTGCT GGAATGTTCA 
    GCTGAGGAGA CCGCGGGGAA ATGGCTGCAA GCAACTGACC TCACTAGAGA AGTATACCAG
    CATTTGGCCC ACTATGTACC CAAAATCTAC TGCAGGGGTC CCAACCCTTT TCCACAGAAA
    GAAGACATGT TGGCACATCA AGTTTTGTTG GGACCAATGG AATGGTACCT TTGTGGTGAA
    GATCCTGAGT TGGGATTTTC CAAACTTGAA CAAACAAATA AACCTTCTCA TCTTTGTGGT
    CGTGTTTTTA AAGTGGGAGA ACCTACATAC TCTTGCAGAG ACTGTGCAGT TGATCCAACT
    TGTGTTTTAT GCATGGAGTG CTTTTTGGGA AGTATTCACA GAGACCACCG ATATAGGATG
    ACAACATCAG GAGGTGGAGG TTTCTGTGAC TGTGGTGATA CTGAAGCTTG GAAAGAGGGT
    CCTCACTGTC AGAAACATGA ACTTAACACC TATGAAACTG AGGAAGAAGA GGATCCCCTT
    GTCCATTTAT CAGAAGATGT GATAGCAAGA ACTTATAACA TTTTTGCTAT TATGTTTCAA
    TATGCGGTAG AGATACTAAC CTGGGAAAAA GAAAGTGAAT TGCCACCAGA TTTAGAGATG
    ATTGAGAAGA GTGACACCTA CTATTGCATG CTGTTTAATG ACGAGGTTCA TACCTATGAA
    CAAGTTATTT ATACTCTTCA GAAAGCTGTT AACTGTACAC AAAAAGAAGC CATTGGCTTC
    GCAACTACAG TAGATCGAGA TGGACGTAGG TCTGTTCGAT ATGGAGAATT CCAGTATTGT
    GAACAAGCAA AATCAATAAT TGTGAGAAAT ACCAGTAGAC AGACAAAGCC ACTCAAAGTT
    CAAGTTATGC ATTCGTCTAT TGTCGCACAT CAGAATTTTG GTTTGAAAGT GTTGTCTTGG
    CTGGGAAGTA TTATTGGATA TTCAGATGGC CTTCGCCGAA TTTTATGTCA AGTTGGTTTA
    CAAGAAGGGC CAGATGGTGA AAACTCTTCT CTAGTGGATA GATTGATGCT TAGTGATTCC
    AAATTGTGGA AAGGTGCTAG GAGTGTGTAT CATCAGTTGT TCATGAGCAG TCTACTTATG
    GATTTGAAAT ACAAGAAACT GTTTGCTGTT CGATTTGCAA AGAACTATGA GCGTTTGCAG
    AGTGATTATG TGACAGATGA CCACGACAGA GAGTTTTCAA TCGCAGACTT CTCGGTTCAG
    ATTTTCACAG TCCCTTCACT TGCTCGGATG CTTATCACAG AAGAAAACCT GATGACCATT
    ATCATTAAGA CTTTCATGGA TCATTTGAGA CACCGAGATG TCCAGGGCAG ATTTCAGTTT
    GAACGATATA CTGCTTTACA AGCCTTTAAG TTTAGGAGGG TTCAGAGTCT TATTTTAGAT
    CTCAAGTATG TGTTAATTAG CAAACCAACT GAATGGTCAG ATGGTCTGAG GCAGAAGTTC
    CTGGAAGGAT TTGATGCCTT TTTGGAATTA CTCAAATGTA TGCAGGGAAT GGATCCAATT
    ACACGTCAAG TAGGACAGCA TATCGAAATG GAACCAGAGT GGGAAGCAGC CTTCACACTA
    CAAATGAAAT TAACACATGT CATTTCGATG ATACAAGACT GGTGTGCTTT AGACGAAAAA
    GTATTAATTG AAGCTTACAA GAAATGCCTT GCTGTATTGA CACAGTGTCA TGGTGGTTTT
    ACTGATGGTG AACAGCCAGT CACATTAAGC ATTTGTGGAC ATTCAGTGGA AACTATCAGA
    TACTGTGTTT CCAAAGAAAA AGTTAGCATT CACCTCCCAG TTTCTCGCTT ACTTGCAGGT
    TTGCATGTGT TGTTAAGCAA AAGTGAAGTG GCATATAAAT TTCCAGAGCT CCTACCTCTA
    AGTGAACTTA GCCCACCCAT GTTGATAGAA CACCCTCTTA GATGTCTTGT TTTATGTGCC
    CAAGTACATG CAGGAATGTG GAGAAGAAAT GGTTTCTCTC TCGTAAACCA GATTTATTAC
    TACCACAATG TGAAATGTAG ACGTGAAATG TTTGATAAGG ATATAGTAAT GCTTCAGACA
    GGTGTCTCCA TGATGGATCC AAATCATTTC CTGATGATAA TGCTTAGCCG CTTTGAACTT
    TATCAGATTT TCAGTACCCC AGATTATGGG AAGAAAATTA GTTCTGAGAC TCCTCATAAG
    GATTTAGTTC AGCAGAACAA CACTCTAATA GAAGAAATGC TATACCTCAT TATAATGCTT
    GTTGGAGAGA GATTTAGTCC TGGAGTTGGA CAGGTTAATG CTACAGATGA GATTAAACGA
    GAGATTATCC ATCAGTTGAG CATCAAACCT ATGGCTCATA GTGAATTGGT AAAGTCTTTA
    CCTGAAGATG AGAACAAGGA GACTGGCATG GAGAGTGTGA TTGAAGCAGT TGCCCATTTC
    AAGAAACCTG GATTAACAGG ACGAGGCATG TATGAACTGA AACCAGAGTG TGCCAAAGAG
    TTCAATTTGT ATTTCTATCA CTTTTCAAGG GCAGAGCAAT CCAAGGCAGA GGAAGCTCAA
    CGGAAATTGA AAAAACAAAA TAAAGAAGAT ACAGCACTTC CGCCTCCAGT TTTGCCCCCA
    TTCTGCCCTC TGTTTGCAAG TCTGGTTAAC ATTTTGCAGT CAGATGTCAT GTTGTTAATC
    ATGAGGACAG TGTTGCAGTG GACTGTAGAA CATAGTGGAC ATATCTGGTC TGAGTCCATG
    CTGCAAAGGG TATTACATTT AATTGGAATG GCACTACAAG AAGAAAAACA GCATTTAGAG
    AATGTCATGG AGGAGCATGT CATAACATTT ACCTTCACAC AGAAGATATC GAAACCTGGT
    GAGGCTCCAA ACAACTCACC TAGTATACTA GCTATGCTGG AAACACTGCA GAATGCTCCC
    TACCTAGAAG TCCACAAAGA TATGATTAAG TGGATATTAA AGACTTTTAA TGCTATTAAG
    AAGTTAAGGG AGAGTTCATC CACCAGTCCT GTGGCAGAGA CAGAAGGAAC CATAATGGAA
    GAGAGTTCGA GAGACAAAGA CAAAGCTGAG AGGAAGAGAA AAGCTGAGAT TGCCAGATTA
    CGCAGAGAGA AGATCATGGC TCAGATGTCT GAGATGCAGC GGCACTTTAT TGATGAGAAC
    AAAGAACTCT TTCAGCAGAC GTTAGAACTG GATTCCTCTG TCTCTGCTGT ACTTGATAAT
    GGCCCTGTGG TTTCAGATAT GACTCTTACA GCATTGGGCC CAGCACAGAC TCGGGTCCCT
    GAACAGAGAC AGTGTGTCAC CTGTATATTG TGTCAGGAAG AGCAAGAGGT GAATGCAGAA
    AGCAAGGCAA TGGTCCTGGC AGCATTTGTG CAGAGATCAA CTGTGTTATC AAAAGACAGA
    AGTAAATTCA TTCAGGATCC AGAAAATTAT GATCCATTAT TCATGCACCC TGATCTGTCT
    TGTGGAATAC ATACTGGTAG CTGTGGGCAT ATTATGCATG CACACTGTTG GCAAAGGTAT
    TTTGATTCCG TTCAAGCTAA AGAACAGCGA AGGCAACAGA GATTACGCTT ACATACAAGC
    TATGATGTAG AAAATGGAGA ATTCCTTTGT CCCCTTTGTG AATGCTTGAG TAATACTGTT
    ATTCCTCTGC TGCTTCCTCC AAGAAATGTT TTTCATGGCA GGTTAAATTT TTCAGACCAA
    CCAAATCTGA CTCAGTGGAT TAGAACAATA TCTCAGCAAA TAAAAGCATT ACAGGTTCTT
    AGGAAAGAAG AAAGTACTCC TATTACTGCC TCATCGAAGA ATTTAGAAAA TATGGATGAA
    TTGCACCTTC CTGAAGGATT TAGACCTGAT TTTCATCCTA AGAACCCTTA TTCTGAAAGC
    ATAAAAGAAA TGTTAGCAAC GTTTGGAACT GCTACTTATA AGGTGGGATT AAAAGTTCAT
    CCCAATGAAG AGGATCCCCG TGTGCCCATA ATGTGTTGGA GTAGTTGTGC ATATACCATC
    CAAAGCATAG AAAGAATTTT AAGTGATGAA GATAAACCAT TGTTTGGTCC TTTGCCTTGT
    AGACTGGATG ACTGTCTCAG GTCACTAACA AGATTTGCTG GAGCACATTG GACAGTGGTG
    TCACTTTCAG TGGTGCAAGG ACACTTTTGT AAACTTTTTG CATCATTGGT GCCTGGTGAC
    AACCACAGAG ACCTTCCATG CATATTAGAT ATCGACATGT TTCATTTATT GGTGGGCTTG
    GTCCTTGCCT TCCCTTCACT GCAGTGTCAG GACTTTTCAG GGATCAGCCT GGGCACTGGG
    GACCTTCACA TTTTCCATCT GGTTACCATG GCACACATCG TTCAGATCTT ACTTACCTCG
    TGTACAGAAA AGAGTGGCAT GGATCAAGAA AATGCTGCTG GCAAAGAAGA AGAACTAGCA
    GTTCTTGCTT TGTGTAAAAT GATTCAGCAG TATACAGGAA GTGCCTTGAA AGAAATGCCA
    TCTGGTTGGC ATCTGTGGAG GAATGTCAAA GCTGGAATCA TGCCTTTTCT GAGGTGTTCT
    GCTTTGTTTT TCCATTACTT AAATGGAGTT CCTTCCCCAC CTGAAATTCA AGCTTCTGGA
    ACAAGCCATT TTGAACATCT ATGTAACTAT CTTTCCCTGC CAAACAACCT TCTTTGTCTT
    TTTCAAGAAA ATAAGGAGAT AATGAAATTA CTGATTGAAA GTTATCCAAG AGAATCTAAC
    AAATTAATAG ACCTTCCAGA GGATTACAGC AGCCTCATTA ATCAAGCATC TAATTTCTCG
    TGCCCCAAAT CAGGTGGTGA TAAGAGCAGA GCCCCAACTA TGTGCCTTGT ATGCGGAACC
    CTGCTGTGCT CCCAGAGCTA CTGCTGCCAG ACGGAGTTGG AAGGGGAGGA CGTGGGGGCA
    TGCACTGCTC ACACCTATTC CTGTGGTTCT GGGGTGGGCA TCTTCCTGAG AGTACGAGAA
    TGTCAAGTAC TATTTTTAGC TGGCAAAACC AAAGGCTGTT TTTATTCTCC TCCTTACCTT
    GATGACTATG GGGAGACTGA CCAGGGACTC AGACGGGGAA ATCCTTTACA TTTGTGCAGA
    GAGCGATTCA AGAAGATTCA GAAGCTCTGG CACCAGCACA GTATCACAGA GGAAATTGGA
    CACGCACAGG AAGCAAACCA GACATTGGTT GGCATTGACT GGCAACACCT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.