CRYBG1 cDNA ORF clone, Bos taurus(cattle)

The following CRYBG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYBG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa51145 XM_003586468.5
Latest version!
Bos taurus crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51145 XM_015464784.1
Latest version!
Bos taurus absent in melanoma 1 (AIM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51146 XM_003582606.4
Latest version!
Bos taurus absent in melanoma 1 (AIM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51146 XM_010808463.3
Latest version!
Bos taurus crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OBa51145 XM_003586468.4 Bos taurus absent in melanoma 1 (AIM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa51146 XM_010808463.2 Bos taurus absent in melanoma 1 (AIM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa51145
    Clone ID Related Accession (Same CDS sequence) XM_015464784.1 , XM_003586468.4 , XM_003586468.5
    Accession Version XM_015464784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product absent in melanoma 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCCACTGT CGCTGCCACC TCAGGGTGAC CACGGGGAGT CCAGTCCGTC CAGGCCCCCC 
    AAAAAGCACA CCTCTTTCCA TATCTGGCGC TCCAAGAAGA AGCAGCAGCC TCCGCGGTCT
    GACTGTGGGG TGTTCATTCC ACACCCGCCC CTGGAGCCCC TCAGAGAGGC CAGAGCTTTG
    GAGGTAGTGG ATGGAGCACA TGTGGCTGGA GAGTATGAAG AATTTACACT GCACTGTGGG
    GAGTCCCAGT TCTTTCACAC CACCTGTGAG ATGCCTGGCG CTTTACCTAC AGAGTCTGGA
    ATCTTTAAAA AGTCCAGAGC ACAACCACCA GAAGAGAACA AAAGGAAGCC AGTTTTGGGA
    AAACTTGGCA CTCTGTTCAC GGCCGGAAGG AGAAGGAACA GCAGAAATGG GTTAGAGAGT
    CCCACCAGCT CAAATACCAA ACCAGGCTCT CCCAAAGAAG TAACCTCTTC CAGACTCCCG
    GAAAGAGAGA CTGAGAAGAG TCAACCTCTG AGCGGCCAAC CAACGCCAAC AGACGCATGC
    GAGGAGGGTT CCCCACAGGA GAAGTGCCAG GAGCCGGAGG GTGAACACCC CGAGGGCTGC
    GTGCAGGCAG CCCCCCCGGA CGCCAAGCGA TCACCTGGCC GGGGCAGCCA TGCAGCAGCG
    GCTGTGCAGC AGGGCGGTGA AAGTGATTCA CCCCAATTAG AACCTCTGGA GGCAGAGGGA
    GAGACCTTCC CAGATGCCAC CGCCACTGCC GAGCAGCTGC ATTCCTCATT AGAGAACTCC
    TCCAAGCGGG AGAACGCAGA GGCGCTCATC CGCAGTCCCG GGGAGGACGC TTCGCCCGGT
    GCTGGCCGCG AGCAGGAGCC CACCCAGGGA TCGAGGGGCG TGCCGGGCTC GCCCGCCGGG
    GAGCATCCCG CCGACGGGGC CCCTCGCTTG TGTGCCCACC GAGGCTCTCC GGAGCCCCAG
    GGTGGGCTGA GCCAAGCCCC GGAGGACTCT TCAGCTCCGC CGGGCGACCC GCTCGCCGAG
    GGTGCGGAGA ACCGGGCGGG CTCAGCACCA GTCAACGTCA AAGCCGAGCC CCCGGGGCAA
    GACTGCCGAC CCCGCGAGCG CGCCCACCCA GCCAAAGTGC TGACTCTGGA CATCTACTTA
    AGTAAGACCG AGGCGGCACA GGTGGACGAG CCGGTCGTGA TCAGCCCGGG GGCGGGAGAT
    TGCGGCGATT GCGACGACAT GGAAAAGAGG TCGAGCGGCC GCAGGTCTGG GAGAAGGAGG
    CGGTCTCAGA GATCCACCGA CTCCCCCGGC GCTGACCCGC CGTTGCCCGA CTGTGAGCCC
    CGGGACGACA CGGTGTTCGA GGACGAGGTG GCGCCAGACG CGGCCGCCGA GAATGGCTGT
    GCAGAAAAGA AAGTGAAATC TCCTCCGCAG GCGGCCCCCA ATGGGGGCGT TGCCTCCGCT
    GCCGGCCTGG AGTCCAAGTC CAGCCCCAGC CCCAAAGGGC AGCTCCGAGG GGAGCCGGAG
    CGGAGCAAAC AGCCGCCGCC CGCCTCCTCC CCCACCAAGA GGAGGGGCCG GAGCCGCGTC
    CCCGAGGCTG TGCCCACCTC GCCCGCAGGC GGCCCGCGGC CTACGGCCAA GGACTCCCCT
    CTCAAGAGGG CGCCAGACCC CGTCCCCAGC CCGGCCGCCA AGGAAAGCGG GGAGGAGGCG
    GCCCGAGTCA TCCCCCGGGA GCTCACGGTT AAGAGCAGCT CGCTACTGCC TGAGATCAAG
    CCCGAGCACA AGAGGGGGCC GCTCCCCCAC CTCTTGGATG GCCGGGGAGA CGGAGGTCGA
    AGCAGAGACT TGGGCAGATC GGCTGGAGGT TCTGACGCCT TTGAGGGCTT GAAGCCCAGG
    AACCACTTCG GTGTGGGCAG ATCGACGGTG ACCACGAAAG TGACCCTCCC TGCCAGGCCC
    AAACACGTGG AACTAAATCT TAAAAACTCT AAGAATCTTG ACAGTTTGGG AAGTGAGCAT
    AATCCATTTA GCCAGCCAGT TCACAAAGGA AACACTGCCA CCAAAATCTC CTTATTTGAA
    AACAAGCGGG CAAACAGCAG CCCAAGACAC ACTGACATTC GAGGTTTGAA GAACACTCCT
    GCGTCTAATA AAACATTTGT CGAGAGGGCA AAGCTGAATT TAGCCAAAAA AGCCAAGGAA
    ATGGAGCTGC CTGAAAAGAA GGTGATGCCA AATAGTCACC AGAACGGTGT GCCGGGGAAG
    CTGTCATCTG CAGAAGCCAA AGTGATGCTT CCTGAAGAGG AGACTCCTGT TTCTCAGCTT
    TCCCAAGGTG ATAAGGTAGT TGTGCAAACA GATGCTGGCT GCCCTTCCGA ACCAGTGGTT
    GCTGCTCTGA CTCCCGTTAA GGACCAGGAG ATCTTAGGAG AGAATGACTC CAAGGCTGCT
    AACAGCAAAA GACTTGAACT TGAAAATATG ACTAATATGG CAGAAAACAC CCCTGCCATT
    CTTGATACCA AAGATCCGCC TCCAACAGCC ATACCAAACC CGCAGCCTGG ATTTTCTGAC
    TCACAGCCCC CAGCTGAGTC ATCTAATGGG CCTTCTCTGT CACTGGCTGC CCCCATTCCT
    GCAGATGATA CCAAAGACAC ATGTGCTCAA GCTCCTGTAA GCAGTTCTCT GTGCACTGAT
    CTCAGAGAGT CAGAAAGCCA TAATGGATGT ACTCTGCCTG TATCTCATCA TAATAATGAG
    ACAATGCCCC CCTCCAAACT TGCAGGAGGA AGAGAAGGAG GAGGGGAGAC AAGCCCTCCT
    TTGTCCCCAG AGCACAGCCC GCAAGCTGTG GGAGATGAGT GTCCATCCAA GGTCCTAGTC
    CAGGTCAGGT CCTTCATGCT CCCTGTGGAG AGCATGGAGG GGGTCTGCTC CCAGATCATC
    ACAGAAAGCT CTGAAGTTAG AGAAGCACAG TTGCCAAGCT GTCACAATAA TGAACTCCAA
    GTGGTTTCCG TTGCAAGTGG TGCTCCCCAG AAAGAGGAAG TACAGGGCAA TAAGGGCACA
    TCACCCAAAC ACATTCACTG CAAAGAGGAA CATATAGTAA AATCTGGCGC ACAAGTCATG
    CCGCCTGAAT CAGAAGCAAC TCTGCCTGTC CAGGCTCAAA GTGAGGACAA CGAAAAACCC
    CTGAAGGCTG AATCCACCGC CACCAGCCTC CCTGACAACA GAAGTCATTT AGAAACCCCC
    CAAAGACCCA GTCAGAGTGC TGTGAATGGC CAGGGCAGTC CTGCCAGTCT TTTGAACATC
    TCTGCTGGTA GTGATGATAG TGTATTCGAT TCTTCTTCTG ACATGGAAAA ATTCACTGAG
    ATTATAAAAA AGATGGACAG CACTGTGTGT ATGCCCCAGA AGAAAAAGAA GACCAGGCTA
    CCAAACTCCC CTGCCCCTCA CTTTGCCATG CCACCTATTC ACGAGGACAA TTTAGAAAAG
    GTGTTTGATC CCAACGTGTT TACCTTTGGT TTGGGGAAGA AAAAGGAAGG CCAGTTGGAA
    ATGTCACCAG CTTTACATTT GATGCAGAAT CTTGACACCA AATCCAAACT GAGGCCCAAA
    CGATCATCCA CTGAACAGAG TGTCCTCTTC AAGTCCTTGC AGACTCATAC TAATGGGAAA
    GATGAGCCCC TGGCAGCTCC AGAAGTCAAT GACAAAGAGA ACAAGGATGT CCAAAATGGT
    GGAGTCAAGA GATCGAGGCT AGAAAAAAGT GCACTTTTCT CAAGCTTGTT ATCTTCTCTA
    CCACAAGACA AAATCTTTTC TCCCTCTGTA ACATCAGTCA ATACCATGAC CACATCTTTC
    GGTACTGCTC ATAGCAGTTC TCTTTCTCGA CCTTCCGTGT TGCAGCCTGT GGCTGAGAGT
    GCCCCACCCT GTAGTACAGA GAAAGAACAA CCAAGTCTGC CAGCCAGTAA CTTAAAGGTC
    TTCAATTTCA ACTCGTCAGA TACGTCTCAT TCGGCATTGA AAAGTCTGAG CCCCATGGAA
    AAGTGCCCGC AAAAAGAGGA AACCAAAAAG GATCTGGATC TGCAAAGCAA CCTTCCCATG
    TCAGAAACTA AATTTTCTGA ATTTTCAAAA CTGAAGACCA ACGGTGATAC GTCTAATCAT
    ACTGAAAGTG TTCTTAAATC AAACTTGCCG AGCTATGGAA GCAGTGACAC AGACTTCACT
    GGTCTCTTCA AAGCCAGCCG GTTTGACCCA AGCATTTCTT TTTCTGGAAT GGCATTATCA
    GATACAACAA CAATTAGAGG AAGCGTCCAG AATAAAATCA ATCCCCGACC TGGAAAAGTG
    GTGATATACA GCGAGCCCGA CATCTCAGAG GCATGTATTG AAGTGTTCAG TGACGTGGAG
    GATTGTAGCA CCTGGAGCCT CTCTCCTGTG ATACTAGTAA AAGTCGTTAG AGGATGTTGG
    ATTTTGTATG AGAAACCAAA TTTTGAAGGG CACTCCATCC CCTTAGAAGA AGGAGAATTG
    GAACTCTCTG GTCTCTGGGG TATAGAAGAT ATTTTGGAGA GAAATGAGGA TGAGGCAGTG
    TCGACTAAGC CCATGGTGAT TGGTTCTATC AGACTTGTGG TCCAGGATTA CAGAGTTAGT
    CAGATTGACT TATTTACTGA ACCAGAAGGG TTAGGACTTC TAAATTCCTA CTTTGATGAT
    ACTGAAGAAA TGCAAGGGTT TGGCATAATA CAGAAGACTT GTTCCATTAA AGTACATTGG
    GGCACATGGC TGATTTATGA AGAGCCTGGA TTTCAGGGTG TCCCTTTTAT CCTGGAACCT
    GGTGAATACC CTGACTTATC CTTCTGGGAT ACAGAGGAGG CATACATTGG ATCCATGCGG
    CCACTGAAAA TGGGTGGCCG TAAAGTTGAA TTCCCTACAG ATCCTAAGGT AGTTATTTAT
    GAAAAGCCTT TCTTTGAAGG AAAATGTATG GAGCTGGAAA CAGAGATGGG TAGGTTTATC
    ATGGAAGGAG GTGAAACAGA AGAAACCACT GGAGATGAAC ATTTGTCATT TGTATCAGTG
    GGATCTATGA AAGTTCTACG AGGCATTTGG GTTGCTTATG AGAAGCCTGA CTTTACAGGT
    CATCAGTATT TGCTTGAAGA AGGAGAATAC AAGGACTGGA ATGACTGGGG AGGCTACAGT
    GGAGAACTGC AGTCTTTACG ACCTATATTA GGTGATTTTT CAAATGCTCA CATGATAATG
    TACAGTGAAA AAAACTTTGG ATCCAAAGGT TCCAGTATTG ATGTATTAGG AATTGTTGCT
    AATTTAAAGG AGACTGGATA CGGAGTGAAG ACACAGTCTA TTAATGTACT GAGTGGAGTA
    TGGGTAGCCT ATGAAAATCC TGACTTCACA GGAGAACAGT ACATACTGGA TAAAGGTTTC
    TATACCAGCT TTGAGGACTG GGGAGGCAAA AACTGTAAGA TCTCCTCTGT TCAACCCATA
    TGTTTGGATT CTTTCACTGG CCCAAGGAGA CGAAATCAGA TTCACTTGTT TTCGGAACCA
    CACTTTCAAG GTTGCAGTCA ATGTTTTGGA GAAACAACAA GTCAAATTGA TGATTCCTTC
    TCTACCAAGT CTTGTAGAGT TTTAGGAGGC AGCTGGGTTG CATATGATGG AGAAAATTTC
    TCTGGTAATC AGTACGTGTT GGAGGAAGGC CACTATCCTT CGCTGTCTGC AATGGGATGC
    CACCCTGGAG CAACTTTCAA GTCTCTTCGT TTTATAGATG TTGAATTTTC TGAGCCAACA
    ATTATTCTTT TTGAAAGAGA AGACTTCAAA GGAAAAAAGA TTGAATTTAA TACAGAAATA
    GTTAATCTCC GATCCCTAGG ATTCAACACA CACATACGCT CTGTCCAGGT GATTGGTGGC
    ATATGGGTTA CTTACGAATA TGGCAATTAC AGGGGCCGAC AGTTCCTACT GTCACCAGCG
    GAAGTGCCTA AGTGGTATGA ATTCAGTGGC TGTCACCAAA TAGGTTCTCT ACGACCTTTT
    GTTCAGAAAC GAATTTATTT TAGACTTCGA AACAAAGCAA CAGGGTTATT CATGTCAACC
    AATGGAAACC TAGAGGATCT GAAGCTTCTC AGAATACAGG TCATGGAGGA TGTCGGAGCT
    GATGACCAGA TCTGGATTTA TCAAGAAGGA TGCATCAAAT GCAGGATAGC AGAGGACTGC
    TGCCTGACCA TCTCGGGGAG CCTCGTAACC TCTGGCTCAA AGCTGGGCCT GGCGCTCGAA
    CAGAATGCTG ACAGTCAGTT CTGGAGCATG AAGCCTGACG GCAGGATCTA CAGCAAGCTT
    AAGCCAAATT TAGTTTTAGA TATCAAAGGG GGTACACAGT ATGATCAAAA TCACCTTATC
    CTTAACACTG TCAGCAAAGA GAAGTTAACA CAAGTGTGGG AAGCCATGGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320270.1
    CDS58..6414
    Translation

    Target ORF information:

    RefSeq Version XM_015464784.1
    Organism Bos taurus(cattle)
    Definition Bos taurus absent in melanoma 1 (AIM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCCACTGT CGCTGCCACC TCAGGGTGAC CACGGGGAGT CCAGTCCGTC CAGGCCCCCC 
    AAAAAGCACA CCTCTTTCCA TATCTGGCGC TCCAAGAAGA AGCAGCAGCC TCCGCGGTCT
    GACTGTGGGG TGTTCATTCC ACACCCGCCC CTGGAGCCCC TCAGAGAGGC CAGAGCTTTG
    GAGGTAGTGG ATGGAGCACA TGTGGCTGGA GAGTATGAAG AATTTACACT GCACTGTGGG
    GAGTCCCAGT TCTTTCACAC CACCTGTGAG ATGCCTGGCG CTTTACCTAC AGAGTCTGGA
    ATCTTTAAAA AGTCCAGAGC ACAACCACCA GAAGAGAACA AAAGGAAGCC AGTTTTGGGA
    AAACTTGGCA CTCTGTTCAC GGCCGGAAGG AGAAGGAACA GCAGAAATGG GTTAGAGAGT
    CCCACCAGCT CAAATACCAA ACCAGGCTCT CCCAAAGAAG TAACCTCTTC CAGACTCCCG
    GAAAGAGAGA CTGAGAAGAG TCAACCTCTG AGCGGCCAAC CAACGCCAAC AGACGCATGC
    GAGGAGGGTT CCCCACAGGA GAAGTGCCAG GAGCCGGAGG GTGAACACCC CGAGGGCTGC
    GTGCAGGCAG CCCCCCCGGA CGCCAAGCGA TCACCTGGCC GGGGCAGCCA TGCAGCAGCG
    GCTGTGCAGC AGGGCGGTGA AAGTGATTCA CCCCAATTAG AACCTCTGGA GGCAGAGGGA
    GAGACCTTCC CAGATGCCAC CGCCACTGCC GAGCAGCTGC ATTCCTCATT AGAGAACTCC
    TCCAAGCGGG AGAACGCAGA GGCGCTCATC CGCAGTCCCG GGGAGGACGC TTCGCCCGGT
    GCTGGCCGCG AGCAGGAGCC CACCCAGGGA TCGAGGGGCG TGCCGGGCTC GCCCGCCGGG
    GAGCATCCCG CCGACGGGGC CCCTCGCTTG TGTGCCCACC GAGGCTCTCC GGAGCCCCAG
    GGTGGGCTGA GCCAAGCCCC GGAGGACTCT TCAGCTCCGC CGGGCGACCC GCTCGCCGAG
    GGTGCGGAGA ACCGGGCGGG CTCAGCACCA GTCAACGTCA AAGCCGAGCC CCCGGGGCAA
    GACTGCCGAC CCCGCGAGCG CGCCCACCCA GCCAAAGTGC TGACTCTGGA CATCTACTTA
    AGTAAGACCG AGGCGGCACA GGTGGACGAG CCGGTCGTGA TCAGCCCGGG GGCGGGAGAT
    TGCGGCGATT GCGACGACAT GGAAAAGAGG TCGAGCGGCC GCAGGTCTGG GAGAAGGAGG
    CGGTCTCAGA GATCCACCGA CTCCCCCGGC GCTGACCCGC CGTTGCCCGA CTGTGAGCCC
    CGGGACGACA CGGTGTTCGA GGACGAGGTG GCGCCAGACG CGGCCGCCGA GAATGGCTGT
    GCAGAAAAGA AAGTGAAATC TCCTCCGCAG GCGGCCCCCA ATGGGGGCGT TGCCTCCGCT
    GCCGGCCTGG AGTCCAAGTC CAGCCCCAGC CCCAAAGGGC AGCTCCGAGG GGAGCCGGAG
    CGGAGCAAAC AGCCGCCGCC CGCCTCCTCC CCCACCAAGA GGAGGGGCCG GAGCCGCGTC
    CCCGAGGCTG TGCCCACCTC GCCCGCAGGC GGCCCGCGGC CTACGGCCAA GGACTCCCCT
    CTCAAGAGGG CGCCAGACCC CGTCCCCAGC CCGGCCGCCA AGGAAAGCGG GGAGGAGGCG
    GCCCGAGTCA TCCCCCGGGA GCTCACGGTT AAGAGCAGCT CGCTACTGCC TGAGATCAAG
    CCCGAGCACA AGAGGGGGCC GCTCCCCCAC CTCTTGGATG GCCGGGGAGA CGGAGGTCGA
    AGCAGAGACT TGGGCAGATC GGCTGGAGGT TCTGACGCCT TTGAGGGCTT GAAGCCCAGG
    AACCACTTCG GTGTGGGCAG ATCGACGGTG ACCACGAAAG TGACCCTCCC TGCCAGGCCC
    AAACACGTGG AACTAAATCT TAAAAACTCT AAGAATCTTG ACAGTTTGGG AAGTGAGCAT
    AATCCATTTA GCCAGCCAGT TCACAAAGGA AACACTGCCA CCAAAATCTC CTTATTTGAA
    AACAAGCGGG CAAACAGCAG CCCAAGACAC ACTGACATTC GAGGTTTGAA GAACACTCCT
    GCGTCTAATA AAACATTTGT CGAGAGGGCA AAGCTGAATT TAGCCAAAAA AGCCAAGGAA
    ATGGAGCTGC CTGAAAAGAA GGTGATGCCA AATAGTCACC AGAACGGTGT GCCGGGGAAG
    CTGTCATCTG CAGAAGCCAA AGTGATGCTT CCTGAAGAGG AGACTCCTGT TTCTCAGCTT
    TCCCAAGGTG ATAAGGTAGT TGTGCAAACA GATGCTGGCT GCCCTTCCGA ACCAGTGGTT
    GCTGCTCTGA CTCCCGTTAA GGACCAGGAG ATCTTAGGAG AGAATGACTC CAAGGCTGCT
    AACAGCAAAA GACTTGAACT TGAAAATATG ACTAATATGG CAGAAAACAC CCCTGCCATT
    CTTGATACCA AAGATCCGCC TCCAACAGCC ATACCAAACC CGCAGCCTGG ATTTTCTGAC
    TCACAGCCCC CAGCTGAGTC ATCTAATGGG CCTTCTCTGT CACTGGCTGC CCCCATTCCT
    GCAGATGATA CCAAAGACAC ATGTGCTCAA GCTCCTGTAA GCAGTTCTCT GTGCACTGAT
    CTCAGAGAGT CAGAAAGCCA TAATGGATGT ACTCTGCCTG TATCTCATCA TAATAATGAG
    ACAATGCCCC CCTCCAAACT TGCAGGAGGA AGAGAAGGAG GAGGGGAGAC AAGCCCTCCT
    TTGTCCCCAG AGCACAGCCC GCAAGCTGTG GGAGATGAGT GTCCATCCAA GGTCCTAGTC
    CAGGTCAGGT CCTTCATGCT CCCTGTGGAG AGCATGGAGG GGGTCTGCTC CCAGATCATC
    ACAGAAAGCT CTGAAGTTAG AGAAGCACAG TTGCCAAGCT GTCACAATAA TGAACTCCAA
    GTGGTTTCCG TTGCAAGTGG TGCTCCCCAG AAAGAGGAAG TACAGGGCAA TAAGGGCACA
    TCACCCAAAC ACATTCACTG CAAAGAGGAA CATATAGTAA AATCTGGCGC ACAAGTCATG
    CCGCCTGAAT CAGAAGCAAC TCTGCCTGTC CAGGCTCAAA GTGAGGACAA CGAAAAACCC
    CTGAAGGCTG AATCCACCGC CACCAGCCTC CCTGACAACA GAAGTCATTT AGAAACCCCC
    CAAAGACCCA GTCAGAGTGC TGTGAATGGC CAGGGCAGTC CTGCCAGTCT TTTGAACATC
    TCTGCTGGTA GTGATGATAG TGTATTCGAT TCTTCTTCTG ACATGGAAAA ATTCACTGAG
    ATTATAAAAA AGATGGACAG CACTGTGTGT ATGCCCCAGA AGAAAAAGAA GACCAGGCTA
    CCAAACTCCC CTGCCCCTCA CTTTGCCATG CCACCTATTC ACGAGGACAA TTTAGAAAAG
    GTGTTTGATC CCAACGTGTT TACCTTTGGT TTGGGGAAGA AAAAGGAAGG CCAGTTGGAA
    ATGTCACCAG CTTTACATTT GATGCAGAAT CTTGACACCA AATCCAAACT GAGGCCCAAA
    CGATCATCCA CTGAACAGAG TGTCCTCTTC AAGTCCTTGC AGACTCATAC TAATGGGAAA
    GATGAGCCCC TGGCAGCTCC AGAAGTCAAT GACAAAGAGA ACAAGGATGT CCAAAATGGT
    GGAGTCAAGA GATCGAGGCT AGAAAAAAGT GCACTTTTCT CAAGCTTGTT ATCTTCTCTA
    CCACAAGACA AAATCTTTTC TCCCTCTGTA ACATCAGTCA ATACCATGAC CACATCTTTC
    GGTACTGCTC ATAGCAGTTC TCTTTCTCGA CCTTCCGTGT TGCAGCCTGT GGCTGAGAGT
    GCCCCACCCT GTAGTACAGA GAAAGAACAA CCAAGTCTGC CAGCCAGTAA CTTAAAGGTC
    TTCAATTTCA ACTCGTCAGA TACGTCTCAT TCGGCATTGA AAAGTCTGAG CCCCATGGAA
    AAGTGCCCGC AAAAAGAGGA AACCAAAAAG GATCTGGATC TGCAAAGCAA CCTTCCCATG
    TCAGAAACTA AATTTTCTGA ATTTTCAAAA CTGAAGACCA ACGGTGATAC GTCTAATCAT
    ACTGAAAGTG TTCTTAAATC AAACTTGCCG AGCTATGGAA GCAGTGACAC AGACTTCACT
    GGTCTCTTCA AAGCCAGCCG GTTTGACCCA AGCATTTCTT TTTCTGGAAT GGCATTATCA
    GATACAACAA CAATTAGAGG AAGCGTCCAG AATAAAATCA ATCCCCGACC TGGAAAAGTG
    GTGATATACA GCGAGCCCGA CATCTCAGAG GCATGTATTG AAGTGTTCAG TGACGTGGAG
    GATTGTAGCA CCTGGAGCCT CTCTCCTGTG ATACTAGTAA AAGTCGTTAG AGGATGTTGG
    ATTTTGTATG AGAAACCAAA TTTTGAAGGG CACTCCATCC CCTTAGAAGA AGGAGAATTG
    GAACTCTCTG GTCTCTGGGG TATAGAAGAT ATTTTGGAGA GAAATGAGGA TGAGGCAGTG
    TCGACTAAGC CCATGGTGAT TGGTTCTATC AGACTTGTGG TCCAGGATTA CAGAGTTAGT
    CAGATTGACT TATTTACTGA ACCAGAAGGG TTAGGACTTC TAAATTCCTA CTTTGATGAT
    ACTGAAGAAA TGCAAGGGTT TGGCATAATA CAGAAGACTT GTTCCATTAA AGTACATTGG
    GGCACATGGC TGATTTATGA AGAGCCTGGA TTTCAGGGTG TCCCTTTTAT CCTGGAACCT
    GGTGAATACC CTGACTTATC CTTCTGGGAT ACAGAGGAGG CATACATTGG ATCCATGCGG
    CCACTGAAAA TGGGTGGCCG TAAAGTTGAA TTCCCTACAG ATCCTAAGGT AGTTATTTAT
    GAAAAGCCTT TCTTTGAAGG AAAATGTATG GAGCTGGAAA CAGAGATGGG TAGGTTTATC
    ATGGAAGGAG GTGAAACAGA AGAAACCACT GGAGATGAAC ATTTGTCATT TGTATCAGTG
    GGATCTATGA AAGTTCTACG AGGCATTTGG GTTGCTTATG AGAAGCCTGA CTTTACAGGT
    CATCAGTATT TGCTTGAAGA AGGAGAATAC AAGGACTGGA ATGACTGGGG AGGCTACAGT
    GGAGAACTGC AGTCTTTACG ACCTATATTA GGTGATTTTT CAAATGCTCA CATGATAATG
    TACAGTGAAA AAAACTTTGG ATCCAAAGGT TCCAGTATTG ATGTATTAGG AATTGTTGCT
    AATTTAAAGG AGACTGGATA CGGAGTGAAG ACACAGTCTA TTAATGTACT GAGTGGAGTA
    TGGGTAGCCT ATGAAAATCC TGACTTCACA GGAGAACAGT ACATACTGGA TAAAGGTTTC
    TATACCAGCT TTGAGGACTG GGGAGGCAAA AACTGTAAGA TCTCCTCTGT TCAACCCATA
    TGTTTGGATT CTTTCACTGG CCCAAGGAGA CGAAATCAGA TTCACTTGTT TTCGGAACCA
    CACTTTCAAG GTTGCAGTCA ATGTTTTGGA GAAACAACAA GTCAAATTGA TGATTCCTTC
    TCTACCAAGT CTTGTAGAGT TTTAGGAGGC AGCTGGGTTG CATATGATGG AGAAAATTTC
    TCTGGTAATC AGTACGTGTT GGAGGAAGGC CACTATCCTT CGCTGTCTGC AATGGGATGC
    CACCCTGGAG CAACTTTCAA GTCTCTTCGT TTTATAGATG TTGAATTTTC TGAGCCAACA
    ATTATTCTTT TTGAAAGAGA AGACTTCAAA GGAAAAAAGA TTGAATTTAA TACAGAAATA
    GTTAATCTCC GATCCCTAGG ATTCAACACA CACATACGCT CTGTCCAGGT GATTGGTGGC
    ATATGGGTTA CTTACGAATA TGGCAATTAC AGGGGCCGAC AGTTCCTACT GTCACCAGCG
    GAAGTGCCTA AGTGGTATGA ATTCAGTGGC TGTCACCAAA TAGGTTCTCT ACGACCTTTT
    GTTCAGAAAC GAATTTATTT TAGACTTCGA AACAAAGCAA CAGGGTTATT CATGTCAACC
    AATGGAAACC TAGAGGATCT GAAGCTTCTC AGAATACAGG TCATGGAGGA TGTCGGAGCT
    GATGACCAGA TCTGGATTTA TCAAGAAGGA TGCATCAAAT GCAGGATAGC AGAGGACTGC
    TGCCTGACCA TCTCGGGGAG CCTCGTAACC TCTGGCTCAA AGCTGGGCCT GGCGCTCGAA
    CAGAATGCTG ACAGTCAGTT CTGGAGCATG AAGCCTGACG GCAGGATCTA CAGCAAGCTT
    AAGCCAAATT TAGTTTTAGA TATCAAAGGG GGTACACAGT ATGATCAAAA TCACCTTATC
    CTTAACACTG TCAGCAAAGA GAAGTTAACA CAAGTGTGGG AAGCCATGGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51145
    Clone ID Related Accession (Same CDS sequence) XM_015464784.1 , XM_003586468.4 , XM_003586468.5
    Accession Version XM_003586468.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product absent in melanoma 1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586468.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCCACTGT CGCTGCCACC TCAGGGTGAC CACGGGGAGT CCAGTCCGTC CAGGCCCCCC 
    AAAAAGCACA CCTCTTTCCA TATCTGGCGC TCCAAGAAGA AGCAGCAGCC TCCGCGGTCT
    GACTGTGGGG TGTTCATTCC ACACCCGCCC CTGGAGCCCC TCAGAGAGGC CAGAGCTTTG
    GAGGTAGTGG ATGGAGCACA TGTGGCTGGA GAGTATGAAG AATTTACACT GCACTGTGGG
    GAGTCCCAGT TCTTTCACAC CACCTGTGAG ATGCCTGGCG CTTTACCTAC AGAGTCTGGA
    ATCTTTAAAA AGTCCAGAGC ACAACCACCA GAAGAGAACA AAAGGAAGCC AGTTTTGGGA
    AAACTTGGCA CTCTGTTCAC GGCCGGAAGG AGAAGGAACA GCAGAAATGG GTTAGAGAGT
    CCCACCAGCT CAAATACCAA ACCAGGCTCT CCCAAAGAAG TAACCTCTTC CAGACTCCCG
    GAAAGAGAGA CTGAGAAGAG TCAACCTCTG AGCGGCCAAC CAACGCCAAC AGACGCATGC
    GAGGAGGGTT CCCCACAGGA GAAGTGCCAG GAGCCGGAGG GTGAACACCC CGAGGGCTGC
    GTGCAGGCAG CCCCCCCGGA CGCCAAGCGA TCACCTGGCC GGGGCAGCCA TGCAGCAGCG
    GCTGTGCAGC AGGGCGGTGA AAGTGATTCA CCCCAATTAG AACCTCTGGA GGCAGAGGGA
    GAGACCTTCC CAGATGCCAC CGCCACTGCC GAGCAGCTGC ATTCCTCATT AGAGAACTCC
    TCCAAGCGGG AGAACGCAGA GGCGCTCATC CGCAGTCCCG GGGAGGACGC TTCGCCCGGT
    GCTGGCCGCG AGCAGGAGCC CACCCAGGGA TCGAGGGGCG TGCCGGGCTC GCCCGCCGGG
    GAGCATCCCG CCGACGGGGC CCCTCGCTTG TGTGCCCACC GAGGCTCTCC GGAGCCCCAG
    GGTGGGCTGA GCCAAGCCCC GGAGGACTCT TCAGCTCCGC CGGGCGACCC GCTCGCCGAG
    GGTGCGGAGA ACCGGGCGGG CTCAGCACCA GTCAACGTCA AAGCCGAGCC CCCGGGGCAA
    GACTGCCGAC CCCGCGAGCG CGCCCACCCA GCCAAAGTGC TGACTCTGGA CATCTACTTA
    AGTAAGACCG AGGCGGCACA GGTGGACGAG CCGGTCGTGA TCAGCCCGGG GGCGGGAGAT
    TGCGGCGATT GCGACGACAT GGAAAAGAGG TCGAGCGGCC GCAGGTCTGG GAGAAGGAGG
    CGGTCTCAGA GATCCACCGA CTCCCCCGGC GCTGACCCGC CGTTGCCCGA CTGTGAGCCC
    CGGGACGACA CGGTGTTCGA GGACGAGGTG GCGCCAGACG CGGCCGCCGA GAATGGCTGT
    GCAGAAAAGA AAGTGAAATC TCCTCCGCAG GCGGCCCCCA ATGGGGGCGT TGCCTCCGCT
    GCCGGCCTGG AGTCCAAGTC CAGCCCCAGC CCCAAAGGGC AGCTCCGAGG GGAGCCGGAG
    CGGAGCAAAC AGCCGCCGCC CGCCTCCTCC CCCACCAAGA GGAGGGGCCG GAGCCGCGTC
    CCCGAGGCTG TGCCCACCTC GCCCGCAGGC GGCCCGCGGC CTACGGCCAA GGACTCCCCT
    CTCAAGAGGG CGCCAGACCC CGTCCCCAGC CCGGCCGCCA AGGAAAGCGG GGAGGAGGCG
    GCCCGAGTCA TCCCCCGGGA GCTCACGGTT AAGAGCAGCT CGCTACTGCC TGAGATCAAG
    CCCGAGCACA AGAGGGGGCC GCTCCCCCAC CTCTTGGATG GCCGGGGAGA CGGAGGTCGA
    AGCAGAGACT TGGGCAGATC GGCTGGAGGT TCTGACGCCT TTGAGGGCTT GAAGCCCAGG
    AACCACTTCG GTGTGGGCAG ATCGACGGTG ACCACGAAAG TGACCCTCCC TGCCAGGCCC
    AAACACGTGG AACTAAATCT TAAAAACTCT AAGAATCTTG ACAGTTTGGG AAGTGAGCAT
    AATCCATTTA GCCAGCCAGT TCACAAAGGA AACACTGCCA CCAAAATCTC CTTATTTGAA
    AACAAGCGGG CAAACAGCAG CCCAAGACAC ACTGACATTC GAGGTTTGAA GAACACTCCT
    GCGTCTAATA AAACATTTGT CGAGAGGGCA AAGCTGAATT TAGCCAAAAA AGCCAAGGAA
    ATGGAGCTGC CTGAAAAGAA GGTGATGCCA AATAGTCACC AGAACGGTGT GCCGGGGAAG
    CTGTCATCTG CAGAAGCCAA AGTGATGCTT CCTGAAGAGG AGACTCCTGT TTCTCAGCTT
    TCCCAAGGTG ATAAGGTAGT TGTGCAAACA GATGCTGGCT GCCCTTCCGA ACCAGTGGTT
    GCTGCTCTGA CTCCCGTTAA GGACCAGGAG ATCTTAGGAG AGAATGACTC CAAGGCTGCT
    AACAGCAAAA GACTTGAACT TGAAAATATG ACTAATATGG CAGAAAACAC CCCTGCCATT
    CTTGATACCA AAGATCCGCC TCCAACAGCC ATACCAAACC CGCAGCCTGG ATTTTCTGAC
    TCACAGCCCC CAGCTGAGTC ATCTAATGGG CCTTCTCTGT CACTGGCTGC CCCCATTCCT
    GCAGATGATA CCAAAGACAC ATGTGCTCAA GCTCCTGTAA GCAGTTCTCT GTGCACTGAT
    CTCAGAGAGT CAGAAAGCCA TAATGGATGT ACTCTGCCTG TATCTCATCA TAATAATGAG
    ACAATGCCCC CCTCCAAACT TGCAGGAGGA AGAGAAGGAG GAGGGGAGAC AAGCCCTCCT
    TTGTCCCCAG AGCACAGCCC GCAAGCTGTG GGAGATGAGT GTCCATCCAA GGTCCTAGTC
    CAGGTCAGGT CCTTCATGCT CCCTGTGGAG AGCATGGAGG GGGTCTGCTC CCAGATCATC
    ACAGAAAGCT CTGAAGTTAG AGAAGCACAG TTGCCAAGCT GTCACAATAA TGAACTCCAA
    GTGGTTTCCG TTGCAAGTGG TGCTCCCCAG AAAGAGGAAG TACAGGGCAA TAAGGGCACA
    TCACCCAAAC ACATTCACTG CAAAGAGGAA CATATAGTAA AATCTGGCGC ACAAGTCATG
    CCGCCTGAAT CAGAAGCAAC TCTGCCTGTC CAGGCTCAAA GTGAGGACAA CGAAAAACCC
    CTGAAGGCTG AATCCACCGC CACCAGCCTC CCTGACAACA GAAGTCATTT AGAAACCCCC
    CAAAGACCCA GTCAGAGTGC TGTGAATGGC CAGGGCAGTC CTGCCAGTCT TTTGAACATC
    TCTGCTGGTA GTGATGATAG TGTATTCGAT TCTTCTTCTG ACATGGAAAA ATTCACTGAG
    ATTATAAAAA AGATGGACAG CACTGTGTGT ATGCCCCAGA AGAAAAAGAA GACCAGGCTA
    CCAAACTCCC CTGCCCCTCA CTTTGCCATG CCACCTATTC ACGAGGACAA TTTAGAAAAG
    GTGTTTGATC CCAACGTGTT TACCTTTGGT TTGGGGAAGA AAAAGGAAGG CCAGTTGGAA
    ATGTCACCAG CTTTACATTT GATGCAGAAT CTTGACACCA AATCCAAACT GAGGCCCAAA
    CGATCATCCA CTGAACAGAG TGTCCTCTTC AAGTCCTTGC AGACTCATAC TAATGGGAAA
    GATGAGCCCC TGGCAGCTCC AGAAGTCAAT GACAAAGAGA ACAAGGATGT CCAAAATGGT
    GGAGTCAAGA GATCGAGGCT AGAAAAAAGT GCACTTTTCT CAAGCTTGTT ATCTTCTCTA
    CCACAAGACA AAATCTTTTC TCCCTCTGTA ACATCAGTCA ATACCATGAC CACATCTTTC
    GGTACTGCTC ATAGCAGTTC TCTTTCTCGA CCTTCCGTGT TGCAGCCTGT GGCTGAGAGT
    GCCCCACCCT GTAGTACAGA GAAAGAACAA CCAAGTCTGC CAGCCAGTAA CTTAAAGGTC
    TTCAATTTCA ACTCGTCAGA TACGTCTCAT TCGGCATTGA AAAGTCTGAG CCCCATGGAA
    AAGTGCCCGC AAAAAGAGGA AACCAAAAAG GATCTGGATC TGCAAAGCAA CCTTCCCATG
    TCAGAAACTA AATTTTCTGA ATTTTCAAAA CTGAAGACCA ACGGTGATAC GTCTAATCAT
    ACTGAAAGTG TTCTTAAATC AAACTTGCCG AGCTATGGAA GCAGTGACAC AGACTTCACT
    GGTCTCTTCA AAGCCAGCCG GTTTGACCCA AGCATTTCTT TTTCTGGAAT GGCATTATCA
    GATACAACAA CAATTAGAGG AAGCGTCCAG AATAAAATCA ATCCCCGACC TGGAAAAGTG
    GTGATATACA GCGAGCCCGA CATCTCAGAG GCATGTATTG AAGTGTTCAG TGACGTGGAG
    GATTGTAGCA CCTGGAGCCT CTCTCCTGTG ATACTAGTAA AAGTCGTTAG AGGATGTTGG
    ATTTTGTATG AGAAACCAAA TTTTGAAGGG CACTCCATCC CCTTAGAAGA AGGAGAATTG
    GAACTCTCTG GTCTCTGGGG TATAGAAGAT ATTTTGGAGA GAAATGAGGA TGAGGCAGTG
    TCGACTAAGC CCATGGTGAT TGGTTCTATC AGACTTGTGG TCCAGGATTA CAGAGTTAGT
    CAGATTGACT TATTTACTGA ACCAGAAGGG TTAGGACTTC TAAATTCCTA CTTTGATGAT
    ACTGAAGAAA TGCAAGGGTT TGGCATAATA CAGAAGACTT GTTCCATTAA AGTACATTGG
    GGCACATGGC TGATTTATGA AGAGCCTGGA TTTCAGGGTG TCCCTTTTAT CCTGGAACCT
    GGTGAATACC CTGACTTATC CTTCTGGGAT ACAGAGGAGG CATACATTGG ATCCATGCGG
    CCACTGAAAA TGGGTGGCCG TAAAGTTGAA TTCCCTACAG ATCCTAAGGT AGTTATTTAT
    GAAAAGCCTT TCTTTGAAGG AAAATGTATG GAGCTGGAAA CAGAGATGGG TAGGTTTATC
    ATGGAAGGAG GTGAAACAGA AGAAACCACT GGAGATGAAC ATTTGTCATT TGTATCAGTG
    GGATCTATGA AAGTTCTACG AGGCATTTGG GTTGCTTATG AGAAGCCTGA CTTTACAGGT
    CATCAGTATT TGCTTGAAGA AGGAGAATAC AAGGACTGGA ATGACTGGGG AGGCTACAGT
    GGAGAACTGC AGTCTTTACG ACCTATATTA GGTGATTTTT CAAATGCTCA CATGATAATG
    TACAGTGAAA AAAACTTTGG ATCCAAAGGT TCCAGTATTG ATGTATTAGG AATTGTTGCT
    AATTTAAAGG AGACTGGATA CGGAGTGAAG ACACAGTCTA TTAATGTACT GAGTGGAGTA
    TGGGTAGCCT ATGAAAATCC TGACTTCACA GGAGAACAGT ACATACTGGA TAAAGGTTTC
    TATACCAGCT TTGAGGACTG GGGAGGCAAA AACTGTAAGA TCTCCTCTGT TCAACCCATA
    TGTTTGGATT CTTTCACTGG CCCAAGGAGA CGAAATCAGA TTCACTTGTT TTCGGAACCA
    CACTTTCAAG GTTGCAGTCA ATGTTTTGGA GAAACAACAA GTCAAATTGA TGATTCCTTC
    TCTACCAAGT CTTGTAGAGT TTTAGGAGGC AGCTGGGTTG CATATGATGG AGAAAATTTC
    TCTGGTAATC AGTACGTGTT GGAGGAAGGC CACTATCCTT CGCTGTCTGC AATGGGATGC
    CACCCTGGAG CAACTTTCAA GTCTCTTCGT TTTATAGATG TTGAATTTTC TGAGCCAACA
    ATTATTCTTT TTGAAAGAGA AGACTTCAAA GGAAAAAAGA TTGAATTTAA TACAGAAATA
    GTTAATCTCC GATCCCTAGG ATTCAACACA CACATACGCT CTGTCCAGGT GATTGGTGGC
    ATATGGGTTA CTTACGAATA TGGCAATTAC AGGGGCCGAC AGTTCCTACT GTCACCAGCG
    GAAGTGCCTA AGTGGTATGA ATTCAGTGGC TGTCACCAAA TAGGTTCTCT ACGACCTTTT
    GTTCAGAAAC GAATTTATTT TAGACTTCGA AACAAAGCAA CAGGGTTATT CATGTCAACC
    AATGGAAACC TAGAGGATCT GAAGCTTCTC AGAATACAGG TCATGGAGGA TGTCGGAGCT
    GATGACCAGA TCTGGATTTA TCAAGAAGGA TGCATCAAAT GCAGGATAGC AGAGGACTGC
    TGCCTGACCA TCTCGGGGAG CCTCGTAACC TCTGGCTCAA AGCTGGGCCT GGCGCTCGAA
    CAGAATGCTG ACAGTCAGTT CTGGAGCATG AAGCCTGACG GCAGGATCTA CAGCAAGCTT
    AAGCCAAATT TAGTTTTAGA TATCAAAGGG GGTACACAGT ATGATCAAAA TCACCTTATC
    CTTAACACTG TCAGCAAAGA GAAGTTAACA CAAGTGTGGG AAGCCATGGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586516.3
    CDS58..6414
    Translation

    Target ORF information:

    RefSeq Version XM_003586468.4
    Organism Bos taurus(cattle)
    Definition Bos taurus absent in melanoma 1 (AIM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586468.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCCACTGT CGCTGCCACC TCAGGGTGAC CACGGGGAGT CCAGTCCGTC CAGGCCCCCC 
    AAAAAGCACA CCTCTTTCCA TATCTGGCGC TCCAAGAAGA AGCAGCAGCC TCCGCGGTCT
    GACTGTGGGG TGTTCATTCC ACACCCGCCC CTGGAGCCCC TCAGAGAGGC CAGAGCTTTG
    GAGGTAGTGG ATGGAGCACA TGTGGCTGGA GAGTATGAAG AATTTACACT GCACTGTGGG
    GAGTCCCAGT TCTTTCACAC CACCTGTGAG ATGCCTGGCG CTTTACCTAC AGAGTCTGGA
    ATCTTTAAAA AGTCCAGAGC ACAACCACCA GAAGAGAACA AAAGGAAGCC AGTTTTGGGA
    AAACTTGGCA CTCTGTTCAC GGCCGGAAGG AGAAGGAACA GCAGAAATGG GTTAGAGAGT
    CCCACCAGCT CAAATACCAA ACCAGGCTCT CCCAAAGAAG TAACCTCTTC CAGACTCCCG
    GAAAGAGAGA CTGAGAAGAG TCAACCTCTG AGCGGCCAAC CAACGCCAAC AGACGCATGC
    GAGGAGGGTT CCCCACAGGA GAAGTGCCAG GAGCCGGAGG GTGAACACCC CGAGGGCTGC
    GTGCAGGCAG CCCCCCCGGA CGCCAAGCGA TCACCTGGCC GGGGCAGCCA TGCAGCAGCG
    GCTGTGCAGC AGGGCGGTGA AAGTGATTCA CCCCAATTAG AACCTCTGGA GGCAGAGGGA
    GAGACCTTCC CAGATGCCAC CGCCACTGCC GAGCAGCTGC ATTCCTCATT AGAGAACTCC
    TCCAAGCGGG AGAACGCAGA GGCGCTCATC CGCAGTCCCG GGGAGGACGC TTCGCCCGGT
    GCTGGCCGCG AGCAGGAGCC CACCCAGGGA TCGAGGGGCG TGCCGGGCTC GCCCGCCGGG
    GAGCATCCCG CCGACGGGGC CCCTCGCTTG TGTGCCCACC GAGGCTCTCC GGAGCCCCAG
    GGTGGGCTGA GCCAAGCCCC GGAGGACTCT TCAGCTCCGC CGGGCGACCC GCTCGCCGAG
    GGTGCGGAGA ACCGGGCGGG CTCAGCACCA GTCAACGTCA AAGCCGAGCC CCCGGGGCAA
    GACTGCCGAC CCCGCGAGCG CGCCCACCCA GCCAAAGTGC TGACTCTGGA CATCTACTTA
    AGTAAGACCG AGGCGGCACA GGTGGACGAG CCGGTCGTGA TCAGCCCGGG GGCGGGAGAT
    TGCGGCGATT GCGACGACAT GGAAAAGAGG TCGAGCGGCC GCAGGTCTGG GAGAAGGAGG
    CGGTCTCAGA GATCCACCGA CTCCCCCGGC GCTGACCCGC CGTTGCCCGA CTGTGAGCCC
    CGGGACGACA CGGTGTTCGA GGACGAGGTG GCGCCAGACG CGGCCGCCGA GAATGGCTGT
    GCAGAAAAGA AAGTGAAATC TCCTCCGCAG GCGGCCCCCA ATGGGGGCGT TGCCTCCGCT
    GCCGGCCTGG AGTCCAAGTC CAGCCCCAGC CCCAAAGGGC AGCTCCGAGG GGAGCCGGAG
    CGGAGCAAAC AGCCGCCGCC CGCCTCCTCC CCCACCAAGA GGAGGGGCCG GAGCCGCGTC
    CCCGAGGCTG TGCCCACCTC GCCCGCAGGC GGCCCGCGGC CTACGGCCAA GGACTCCCCT
    CTCAAGAGGG CGCCAGACCC CGTCCCCAGC CCGGCCGCCA AGGAAAGCGG GGAGGAGGCG
    GCCCGAGTCA TCCCCCGGGA GCTCACGGTT AAGAGCAGCT CGCTACTGCC TGAGATCAAG
    CCCGAGCACA AGAGGGGGCC GCTCCCCCAC CTCTTGGATG GCCGGGGAGA CGGAGGTCGA
    AGCAGAGACT TGGGCAGATC GGCTGGAGGT TCTGACGCCT TTGAGGGCTT GAAGCCCAGG
    AACCACTTCG GTGTGGGCAG ATCGACGGTG ACCACGAAAG TGACCCTCCC TGCCAGGCCC
    AAACACGTGG AACTAAATCT TAAAAACTCT AAGAATCTTG ACAGTTTGGG AAGTGAGCAT
    AATCCATTTA GCCAGCCAGT TCACAAAGGA AACACTGCCA CCAAAATCTC CTTATTTGAA
    AACAAGCGGG CAAACAGCAG CCCAAGACAC ACTGACATTC GAGGTTTGAA GAACACTCCT
    GCGTCTAATA AAACATTTGT CGAGAGGGCA AAGCTGAATT TAGCCAAAAA AGCCAAGGAA
    ATGGAGCTGC CTGAAAAGAA GGTGATGCCA AATAGTCACC AGAACGGTGT GCCGGGGAAG
    CTGTCATCTG CAGAAGCCAA AGTGATGCTT CCTGAAGAGG AGACTCCTGT TTCTCAGCTT
    TCCCAAGGTG ATAAGGTAGT TGTGCAAACA GATGCTGGCT GCCCTTCCGA ACCAGTGGTT
    GCTGCTCTGA CTCCCGTTAA GGACCAGGAG ATCTTAGGAG AGAATGACTC CAAGGCTGCT
    AACAGCAAAA GACTTGAACT TGAAAATATG ACTAATATGG CAGAAAACAC CCCTGCCATT
    CTTGATACCA AAGATCCGCC TCCAACAGCC ATACCAAACC CGCAGCCTGG ATTTTCTGAC
    TCACAGCCCC CAGCTGAGTC ATCTAATGGG CCTTCTCTGT CACTGGCTGC CCCCATTCCT
    GCAGATGATA CCAAAGACAC ATGTGCTCAA GCTCCTGTAA GCAGTTCTCT GTGCACTGAT
    CTCAGAGAGT CAGAAAGCCA TAATGGATGT ACTCTGCCTG TATCTCATCA TAATAATGAG
    ACAATGCCCC CCTCCAAACT TGCAGGAGGA AGAGAAGGAG GAGGGGAGAC AAGCCCTCCT
    TTGTCCCCAG AGCACAGCCC GCAAGCTGTG GGAGATGAGT GTCCATCCAA GGTCCTAGTC
    CAGGTCAGGT CCTTCATGCT CCCTGTGGAG AGCATGGAGG GGGTCTGCTC CCAGATCATC
    ACAGAAAGCT CTGAAGTTAG AGAAGCACAG TTGCCAAGCT GTCACAATAA TGAACTCCAA
    GTGGTTTCCG TTGCAAGTGG TGCTCCCCAG AAAGAGGAAG TACAGGGCAA TAAGGGCACA
    TCACCCAAAC ACATTCACTG CAAAGAGGAA CATATAGTAA AATCTGGCGC ACAAGTCATG
    CCGCCTGAAT CAGAAGCAAC TCTGCCTGTC CAGGCTCAAA GTGAGGACAA CGAAAAACCC
    CTGAAGGCTG AATCCACCGC CACCAGCCTC CCTGACAACA GAAGTCATTT AGAAACCCCC
    CAAAGACCCA GTCAGAGTGC TGTGAATGGC CAGGGCAGTC CTGCCAGTCT TTTGAACATC
    TCTGCTGGTA GTGATGATAG TGTATTCGAT TCTTCTTCTG ACATGGAAAA ATTCACTGAG
    ATTATAAAAA AGATGGACAG CACTGTGTGT ATGCCCCAGA AGAAAAAGAA GACCAGGCTA
    CCAAACTCCC CTGCCCCTCA CTTTGCCATG CCACCTATTC ACGAGGACAA TTTAGAAAAG
    GTGTTTGATC CCAACGTGTT TACCTTTGGT TTGGGGAAGA AAAAGGAAGG CCAGTTGGAA
    ATGTCACCAG CTTTACATTT GATGCAGAAT CTTGACACCA AATCCAAACT GAGGCCCAAA
    CGATCATCCA CTGAACAGAG TGTCCTCTTC AAGTCCTTGC AGACTCATAC TAATGGGAAA
    GATGAGCCCC TGGCAGCTCC AGAAGTCAAT GACAAAGAGA ACAAGGATGT CCAAAATGGT
    GGAGTCAAGA GATCGAGGCT AGAAAAAAGT GCACTTTTCT CAAGCTTGTT ATCTTCTCTA
    CCACAAGACA AAATCTTTTC TCCCTCTGTA ACATCAGTCA ATACCATGAC CACATCTTTC
    GGTACTGCTC ATAGCAGTTC TCTTTCTCGA CCTTCCGTGT TGCAGCCTGT GGCTGAGAGT
    GCCCCACCCT GTAGTACAGA GAAAGAACAA CCAAGTCTGC CAGCCAGTAA CTTAAAGGTC
    TTCAATTTCA ACTCGTCAGA TACGTCTCAT TCGGCATTGA AAAGTCTGAG CCCCATGGAA
    AAGTGCCCGC AAAAAGAGGA AACCAAAAAG GATCTGGATC TGCAAAGCAA CCTTCCCATG
    TCAGAAACTA AATTTTCTGA ATTTTCAAAA CTGAAGACCA ACGGTGATAC GTCTAATCAT
    ACTGAAAGTG TTCTTAAATC AAACTTGCCG AGCTATGGAA GCAGTGACAC AGACTTCACT
    GGTCTCTTCA AAGCCAGCCG GTTTGACCCA AGCATTTCTT TTTCTGGAAT GGCATTATCA
    GATACAACAA CAATTAGAGG AAGCGTCCAG AATAAAATCA ATCCCCGACC TGGAAAAGTG
    GTGATATACA GCGAGCCCGA CATCTCAGAG GCATGTATTG AAGTGTTCAG TGACGTGGAG
    GATTGTAGCA CCTGGAGCCT CTCTCCTGTG ATACTAGTAA AAGTCGTTAG AGGATGTTGG
    ATTTTGTATG AGAAACCAAA TTTTGAAGGG CACTCCATCC CCTTAGAAGA AGGAGAATTG
    GAACTCTCTG GTCTCTGGGG TATAGAAGAT ATTTTGGAGA GAAATGAGGA TGAGGCAGTG
    TCGACTAAGC CCATGGTGAT TGGTTCTATC AGACTTGTGG TCCAGGATTA CAGAGTTAGT
    CAGATTGACT TATTTACTGA ACCAGAAGGG TTAGGACTTC TAAATTCCTA CTTTGATGAT
    ACTGAAGAAA TGCAAGGGTT TGGCATAATA CAGAAGACTT GTTCCATTAA AGTACATTGG
    GGCACATGGC TGATTTATGA AGAGCCTGGA TTTCAGGGTG TCCCTTTTAT CCTGGAACCT
    GGTGAATACC CTGACTTATC CTTCTGGGAT ACAGAGGAGG CATACATTGG ATCCATGCGG
    CCACTGAAAA TGGGTGGCCG TAAAGTTGAA TTCCCTACAG ATCCTAAGGT AGTTATTTAT
    GAAAAGCCTT TCTTTGAAGG AAAATGTATG GAGCTGGAAA CAGAGATGGG TAGGTTTATC
    ATGGAAGGAG GTGAAACAGA AGAAACCACT GGAGATGAAC ATTTGTCATT TGTATCAGTG
    GGATCTATGA AAGTTCTACG AGGCATTTGG GTTGCTTATG AGAAGCCTGA CTTTACAGGT
    CATCAGTATT TGCTTGAAGA AGGAGAATAC AAGGACTGGA ATGACTGGGG AGGCTACAGT
    GGAGAACTGC AGTCTTTACG ACCTATATTA GGTGATTTTT CAAATGCTCA CATGATAATG
    TACAGTGAAA AAAACTTTGG ATCCAAAGGT TCCAGTATTG ATGTATTAGG AATTGTTGCT
    AATTTAAAGG AGACTGGATA CGGAGTGAAG ACACAGTCTA TTAATGTACT GAGTGGAGTA
    TGGGTAGCCT ATGAAAATCC TGACTTCACA GGAGAACAGT ACATACTGGA TAAAGGTTTC
    TATACCAGCT TTGAGGACTG GGGAGGCAAA AACTGTAAGA TCTCCTCTGT TCAACCCATA
    TGTTTGGATT CTTTCACTGG CCCAAGGAGA CGAAATCAGA TTCACTTGTT TTCGGAACCA
    CACTTTCAAG GTTGCAGTCA ATGTTTTGGA GAAACAACAA GTCAAATTGA TGATTCCTTC
    TCTACCAAGT CTTGTAGAGT TTTAGGAGGC AGCTGGGTTG CATATGATGG AGAAAATTTC
    TCTGGTAATC AGTACGTGTT GGAGGAAGGC CACTATCCTT CGCTGTCTGC AATGGGATGC
    CACCCTGGAG CAACTTTCAA GTCTCTTCGT TTTATAGATG TTGAATTTTC TGAGCCAACA
    ATTATTCTTT TTGAAAGAGA AGACTTCAAA GGAAAAAAGA TTGAATTTAA TACAGAAATA
    GTTAATCTCC GATCCCTAGG ATTCAACACA CACATACGCT CTGTCCAGGT GATTGGTGGC
    ATATGGGTTA CTTACGAATA TGGCAATTAC AGGGGCCGAC AGTTCCTACT GTCACCAGCG
    GAAGTGCCTA AGTGGTATGA ATTCAGTGGC TGTCACCAAA TAGGTTCTCT ACGACCTTTT
    GTTCAGAAAC GAATTTATTT TAGACTTCGA AACAAAGCAA CAGGGTTATT CATGTCAACC
    AATGGAAACC TAGAGGATCT GAAGCTTCTC AGAATACAGG TCATGGAGGA TGTCGGAGCT
    GATGACCAGA TCTGGATTTA TCAAGAAGGA TGCATCAAAT GCAGGATAGC AGAGGACTGC
    TGCCTGACCA TCTCGGGGAG CCTCGTAACC TCTGGCTCAA AGCTGGGCCT GGCGCTCGAA
    CAGAATGCTG ACAGTCAGTT CTGGAGCATG AAGCCTGACG GCAGGATCTA CAGCAAGCTT
    AAGCCAAATT TAGTTTTAGA TATCAAAGGG GGTACACAGT ATGATCAAAA TCACCTTATC
    CTTAACACTG TCAGCAAAGA GAAGTTAACA CAAGTGTGGG AAGCCATGGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51145
    Clone ID Related Accession (Same CDS sequence) XM_015464784.1 , XM_003586468.4 , XM_003586468.5
    Accession Version XM_003586468.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta/gamma crystallin domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586468.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCCACTGT CGCTGCCACC TCAGGGTGAC CACGGGGAGT CCAGTCCGTC CAGGCCCCCC 
    AAAAAGCACA CCTCTTTCCA TATCTGGCGC TCCAAGAAGA AGCAGCAGCC TCCGCGGTCT
    GACTGTGGGG TGTTCATTCC ACACCCGCCC CTGGAGCCCC TCAGAGAGGC CAGAGCTTTG
    GAGGTAGTGG ATGGAGCACA TGTGGCTGGA GAGTATGAAG AATTTACACT GCACTGTGGG
    GAGTCCCAGT TCTTTCACAC CACCTGTGAG ATGCCTGGCG CTTTACCTAC AGAGTCTGGA
    ATCTTTAAAA AGTCCAGAGC ACAACCACCA GAAGAGAACA AAAGGAAGCC AGTTTTGGGA
    AAACTTGGCA CTCTGTTCAC GGCCGGAAGG AGAAGGAACA GCAGAAATGG GTTAGAGAGT
    CCCACCAGCT CAAATACCAA ACCAGGCTCT CCCAAAGAAG TAACCTCTTC CAGACTCCCG
    GAAAGAGAGA CTGAGAAGAG TCAACCTCTG AGCGGCCAAC CAACGCCAAC AGACGCATGC
    GAGGAGGGTT CCCCACAGGA GAAGTGCCAG GAGCCGGAGG GTGAACACCC CGAGGGCTGC
    GTGCAGGCAG CCCCCCCGGA CGCCAAGCGA TCACCTGGCC GGGGCAGCCA TGCAGCAGCG
    GCTGTGCAGC AGGGCGGTGA AAGTGATTCA CCCCAATTAG AACCTCTGGA GGCAGAGGGA
    GAGACCTTCC CAGATGCCAC CGCCACTGCC GAGCAGCTGC ATTCCTCATT AGAGAACTCC
    TCCAAGCGGG AGAACGCAGA GGCGCTCATC CGCAGTCCCG GGGAGGACGC TTCGCCCGGT
    GCTGGCCGCG AGCAGGAGCC CACCCAGGGA TCGAGGGGCG TGCCGGGCTC GCCCGCCGGG
    GAGCATCCCG CCGACGGGGC CCCTCGCTTG TGTGCCCACC GAGGCTCTCC GGAGCCCCAG
    GGTGGGCTGA GCCAAGCCCC GGAGGACTCT TCAGCTCCGC CGGGCGACCC GCTCGCCGAG
    GGTGCGGAGA ACCGGGCGGG CTCAGCACCA GTCAACGTCA AAGCCGAGCC CCCGGGGCAA
    GACTGCCGAC CCCGCGAGCG CGCCCACCCA GCCAAAGTGC TGACTCTGGA CATCTACTTA
    AGTAAGACCG AGGCGGCACA GGTGGACGAG CCGGTCGTGA TCAGCCCGGG GGCGGGAGAT
    TGCGGCGATT GCGACGACAT GGAAAAGAGG TCGAGCGGCC GCAGGTCTGG GAGAAGGAGG
    CGGTCTCAGA GATCCACCGA CTCCCCCGGC GCTGACCCGC CGTTGCCCGA CTGTGAGCCC
    CGGGACGACA CGGTGTTCGA GGACGAGGTG GCGCCAGACG CGGCCGCCGA GAATGGCTGT
    GCAGAAAAGA AAGTGAAATC TCCTCCGCAG GCGGCCCCCA ATGGGGGCGT TGCCTCCGCT
    GCCGGCCTGG AGTCCAAGTC CAGCCCCAGC CCCAAAGGGC AGCTCCGAGG GGAGCCGGAG
    CGGAGCAAAC AGCCGCCGCC CGCCTCCTCC CCCACCAAGA GGAGGGGCCG GAGCCGCGTC
    CCCGAGGCTG TGCCCACCTC GCCCGCAGGC GGCCCGCGGC CTACGGCCAA GGACTCCCCT
    CTCAAGAGGG CGCCAGACCC CGTCCCCAGC CCGGCCGCCA AGGAAAGCGG GGAGGAGGCG
    GCCCGAGTCA TCCCCCGGGA GCTCACGGTT AAGAGCAGCT CGCTACTGCC TGAGATCAAG
    CCCGAGCACA AGAGGGGGCC GCTCCCCCAC CTCTTGGATG GCCGGGGAGA CGGAGGTCGA
    AGCAGAGACT TGGGCAGATC GGCTGGAGGT TCTGACGCCT TTGAGGGCTT GAAGCCCAGG
    AACCACTTCG GTGTGGGCAG ATCGACGGTG ACCACGAAAG TGACCCTCCC TGCCAGGCCC
    AAACACGTGG AACTAAATCT TAAAAACTCT AAGAATCTTG ACAGTTTGGG AAGTGAGCAT
    AATCCATTTA GCCAGCCAGT TCACAAAGGA AACACTGCCA CCAAAATCTC CTTATTTGAA
    AACAAGCGGG CAAACAGCAG CCCAAGACAC ACTGACATTC GAGGTTTGAA GAACACTCCT
    GCGTCTAATA AAACATTTGT CGAGAGGGCA AAGCTGAATT TAGCCAAAAA AGCCAAGGAA
    ATGGAGCTGC CTGAAAAGAA GGTGATGCCA AATAGTCACC AGAACGGTGT GCCGGGGAAG
    CTGTCATCTG CAGAAGCCAA AGTGATGCTT CCTGAAGAGG AGACTCCTGT TTCTCAGCTT
    TCCCAAGGTG ATAAGGTAGT TGTGCAAACA GATGCTGGCT GCCCTTCCGA ACCAGTGGTT
    GCTGCTCTGA CTCCCGTTAA GGACCAGGAG ATCTTAGGAG AGAATGACTC CAAGGCTGCT
    AACAGCAAAA GACTTGAACT TGAAAATATG ACTAATATGG CAGAAAACAC CCCTGCCATT
    CTTGATACCA AAGATCCGCC TCCAACAGCC ATACCAAACC CGCAGCCTGG ATTTTCTGAC
    TCACAGCCCC CAGCTGAGTC ATCTAATGGG CCTTCTCTGT CACTGGCTGC CCCCATTCCT
    GCAGATGATA CCAAAGACAC ATGTGCTCAA GCTCCTGTAA GCAGTTCTCT GTGCACTGAT
    CTCAGAGAGT CAGAAAGCCA TAATGGATGT ACTCTGCCTG TATCTCATCA TAATAATGAG
    ACAATGCCCC CCTCCAAACT TGCAGGAGGA AGAGAAGGAG GAGGGGAGAC AAGCCCTCCT
    TTGTCCCCAG AGCACAGCCC GCAAGCTGTG GGAGATGAGT GTCCATCCAA GGTCCTAGTC
    CAGGTCAGGT CCTTCATGCT CCCTGTGGAG AGCATGGAGG GGGTCTGCTC CCAGATCATC
    ACAGAAAGCT CTGAAGTTAG AGAAGCACAG TTGCCAAGCT GTCACAATAA TGAACTCCAA
    GTGGTTTCCG TTGCAAGTGG TGCTCCCCAG AAAGAGGAAG TACAGGGCAA TAAGGGCACA
    TCACCCAAAC ACATTCACTG CAAAGAGGAA CATATAGTAA AATCTGGCGC ACAAGTCATG
    CCGCCTGAAT CAGAAGCAAC TCTGCCTGTC CAGGCTCAAA GTGAGGACAA CGAAAAACCC
    CTGAAGGCTG AATCCACCGC CACCAGCCTC CCTGACAACA GAAGTCATTT AGAAACCCCC
    CAAAGACCCA GTCAGAGTGC TGTGAATGGC CAGGGCAGTC CTGCCAGTCT TTTGAACATC
    TCTGCTGGTA GTGATGATAG TGTATTCGAT TCTTCTTCTG ACATGGAAAA ATTCACTGAG
    ATTATAAAAA AGATGGACAG CACTGTGTGT ATGCCCCAGA AGAAAAAGAA GACCAGGCTA
    CCAAACTCCC CTGCCCCTCA CTTTGCCATG CCACCTATTC ACGAGGACAA TTTAGAAAAG
    GTGTTTGATC CCAACGTGTT TACCTTTGGT TTGGGGAAGA AAAAGGAAGG CCAGTTGGAA
    ATGTCACCAG CTTTACATTT GATGCAGAAT CTTGACACCA AATCCAAACT GAGGCCCAAA
    CGATCATCCA CTGAACAGAG TGTCCTCTTC AAGTCCTTGC AGACTCATAC TAATGGGAAA
    GATGAGCCCC TGGCAGCTCC AGAAGTCAAT GACAAAGAGA ACAAGGATGT CCAAAATGGT
    GGAGTCAAGA GATCGAGGCT AGAAAAAAGT GCACTTTTCT CAAGCTTGTT ATCTTCTCTA
    CCACAAGACA AAATCTTTTC TCCCTCTGTA ACATCAGTCA ATACCATGAC CACATCTTTC
    GGTACTGCTC ATAGCAGTTC TCTTTCTCGA CCTTCCGTGT TGCAGCCTGT GGCTGAGAGT
    GCCCCACCCT GTAGTACAGA GAAAGAACAA CCAAGTCTGC CAGCCAGTAA CTTAAAGGTC
    TTCAATTTCA ACTCGTCAGA TACGTCTCAT TCGGCATTGA AAAGTCTGAG CCCCATGGAA
    AAGTGCCCGC AAAAAGAGGA AACCAAAAAG GATCTGGATC TGCAAAGCAA CCTTCCCATG
    TCAGAAACTA AATTTTCTGA ATTTTCAAAA CTGAAGACCA ACGGTGATAC GTCTAATCAT
    ACTGAAAGTG TTCTTAAATC AAACTTGCCG AGCTATGGAA GCAGTGACAC AGACTTCACT
    GGTCTCTTCA AAGCCAGCCG GTTTGACCCA AGCATTTCTT TTTCTGGAAT GGCATTATCA
    GATACAACAA CAATTAGAGG AAGCGTCCAG AATAAAATCA ATCCCCGACC TGGAAAAGTG
    GTGATATACA GCGAGCCCGA CATCTCAGAG GCATGTATTG AAGTGTTCAG TGACGTGGAG
    GATTGTAGCA CCTGGAGCCT CTCTCCTGTG ATACTAGTAA AAGTCGTTAG AGGATGTTGG
    ATTTTGTATG AGAAACCAAA TTTTGAAGGG CACTCCATCC CCTTAGAAGA AGGAGAATTG
    GAACTCTCTG GTCTCTGGGG TATAGAAGAT ATTTTGGAGA GAAATGAGGA TGAGGCAGTG
    TCGACTAAGC CCATGGTGAT TGGTTCTATC AGACTTGTGG TCCAGGATTA CAGAGTTAGT
    CAGATTGACT TATTTACTGA ACCAGAAGGG TTAGGACTTC TAAATTCCTA CTTTGATGAT
    ACTGAAGAAA TGCAAGGGTT TGGCATAATA CAGAAGACTT GTTCCATTAA AGTACATTGG
    GGCACATGGC TGATTTATGA AGAGCCTGGA TTTCAGGGTG TCCCTTTTAT CCTGGAACCT
    GGTGAATACC CTGACTTATC CTTCTGGGAT ACAGAGGAGG CATACATTGG ATCCATGCGG
    CCACTGAAAA TGGGTGGCCG TAAAGTTGAA TTCCCTACAG ATCCTAAGGT AGTTATTTAT
    GAAAAGCCTT TCTTTGAAGG AAAATGTATG GAGCTGGAAA CAGAGATGGG TAGGTTTATC
    ATGGAAGGAG GTGAAACAGA AGAAACCACT GGAGATGAAC ATTTGTCATT TGTATCAGTG
    GGATCTATGA AAGTTCTACG AGGCATTTGG GTTGCTTATG AGAAGCCTGA CTTTACAGGT
    CATCAGTATT TGCTTGAAGA AGGAGAATAC AAGGACTGGA ATGACTGGGG AGGCTACAGT
    GGAGAACTGC AGTCTTTACG ACCTATATTA GGTGATTTTT CAAATGCTCA CATGATAATG
    TACAGTGAAA AAAACTTTGG ATCCAAAGGT TCCAGTATTG ATGTATTAGG AATTGTTGCT
    AATTTAAAGG AGACTGGATA CGGAGTGAAG ACACAGTCTA TTAATGTACT GAGTGGAGTA
    TGGGTAGCCT ATGAAAATCC TGACTTCACA GGAGAACAGT ACATACTGGA TAAAGGTTTC
    TATACCAGCT TTGAGGACTG GGGAGGCAAA AACTGTAAGA TCTCCTCTGT TCAACCCATA
    TGTTTGGATT CTTTCACTGG CCCAAGGAGA CGAAATCAGA TTCACTTGTT TTCGGAACCA
    CACTTTCAAG GTTGCAGTCA ATGTTTTGGA GAAACAACAA GTCAAATTGA TGATTCCTTC
    TCTACCAAGT CTTGTAGAGT TTTAGGAGGC AGCTGGGTTG CATATGATGG AGAAAATTTC
    TCTGGTAATC AGTACGTGTT GGAGGAAGGC CACTATCCTT CGCTGTCTGC AATGGGATGC
    CACCCTGGAG CAACTTTCAA GTCTCTTCGT TTTATAGATG TTGAATTTTC TGAGCCAACA
    ATTATTCTTT TTGAAAGAGA AGACTTCAAA GGAAAAAAGA TTGAATTTAA TACAGAAATA
    GTTAATCTCC GATCCCTAGG ATTCAACACA CACATACGCT CTGTCCAGGT GATTGGTGGC
    ATATGGGTTA CTTACGAATA TGGCAATTAC AGGGGCCGAC AGTTCCTACT GTCACCAGCG
    GAAGTGCCTA AGTGGTATGA ATTCAGTGGC TGTCACCAAA TAGGTTCTCT ACGACCTTTT
    GTTCAGAAAC GAATTTATTT TAGACTTCGA AACAAAGCAA CAGGGTTATT CATGTCAACC
    AATGGAAACC TAGAGGATCT GAAGCTTCTC AGAATACAGG TCATGGAGGA TGTCGGAGCT
    GATGACCAGA TCTGGATTTA TCAAGAAGGA TGCATCAAAT GCAGGATAGC AGAGGACTGC
    TGCCTGACCA TCTCGGGGAG CCTCGTAACC TCTGGCTCAA AGCTGGGCCT GGCGCTCGAA
    CAGAATGCTG ACAGTCAGTT CTGGAGCATG AAGCCTGACG GCAGGATCTA CAGCAAGCTT
    AAGCCAAATT TAGTTTTAGA TATCAAAGGG GGTACACAGT ATGATCAAAA TCACCTTATC
    CTTAACACTG TCAGCAAAGA GAAGTTAACA CAAGTGTGGG AAGCCATGGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586516.3
    CDS82..6438
    Translation

    Target ORF information:

    RefSeq Version XM_003586468.5
    Organism Bos taurus(cattle)
    Definition Bos taurus crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586468.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCCACTGT CGCTGCCACC TCAGGGTGAC CACGGGGAGT CCAGTCCGTC CAGGCCCCCC 
    AAAAAGCACA CCTCTTTCCA TATCTGGCGC TCCAAGAAGA AGCAGCAGCC TCCGCGGTCT
    GACTGTGGGG TGTTCATTCC ACACCCGCCC CTGGAGCCCC TCAGAGAGGC CAGAGCTTTG
    GAGGTAGTGG ATGGAGCACA TGTGGCTGGA GAGTATGAAG AATTTACACT GCACTGTGGG
    GAGTCCCAGT TCTTTCACAC CACCTGTGAG ATGCCTGGCG CTTTACCTAC AGAGTCTGGA
    ATCTTTAAAA AGTCCAGAGC ACAACCACCA GAAGAGAACA AAAGGAAGCC AGTTTTGGGA
    AAACTTGGCA CTCTGTTCAC GGCCGGAAGG AGAAGGAACA GCAGAAATGG GTTAGAGAGT
    CCCACCAGCT CAAATACCAA ACCAGGCTCT CCCAAAGAAG TAACCTCTTC CAGACTCCCG
    GAAAGAGAGA CTGAGAAGAG TCAACCTCTG AGCGGCCAAC CAACGCCAAC AGACGCATGC
    GAGGAGGGTT CCCCACAGGA GAAGTGCCAG GAGCCGGAGG GTGAACACCC CGAGGGCTGC
    GTGCAGGCAG CCCCCCCGGA CGCCAAGCGA TCACCTGGCC GGGGCAGCCA TGCAGCAGCG
    GCTGTGCAGC AGGGCGGTGA AAGTGATTCA CCCCAATTAG AACCTCTGGA GGCAGAGGGA
    GAGACCTTCC CAGATGCCAC CGCCACTGCC GAGCAGCTGC ATTCCTCATT AGAGAACTCC
    TCCAAGCGGG AGAACGCAGA GGCGCTCATC CGCAGTCCCG GGGAGGACGC TTCGCCCGGT
    GCTGGCCGCG AGCAGGAGCC CACCCAGGGA TCGAGGGGCG TGCCGGGCTC GCCCGCCGGG
    GAGCATCCCG CCGACGGGGC CCCTCGCTTG TGTGCCCACC GAGGCTCTCC GGAGCCCCAG
    GGTGGGCTGA GCCAAGCCCC GGAGGACTCT TCAGCTCCGC CGGGCGACCC GCTCGCCGAG
    GGTGCGGAGA ACCGGGCGGG CTCAGCACCA GTCAACGTCA AAGCCGAGCC CCCGGGGCAA
    GACTGCCGAC CCCGCGAGCG CGCCCACCCA GCCAAAGTGC TGACTCTGGA CATCTACTTA
    AGTAAGACCG AGGCGGCACA GGTGGACGAG CCGGTCGTGA TCAGCCCGGG GGCGGGAGAT
    TGCGGCGATT GCGACGACAT GGAAAAGAGG TCGAGCGGCC GCAGGTCTGG GAGAAGGAGG
    CGGTCTCAGA GATCCACCGA CTCCCCCGGC GCTGACCCGC CGTTGCCCGA CTGTGAGCCC
    CGGGACGACA CGGTGTTCGA GGACGAGGTG GCGCCAGACG CGGCCGCCGA GAATGGCTGT
    GCAGAAAAGA AAGTGAAATC TCCTCCGCAG GCGGCCCCCA ATGGGGGCGT TGCCTCCGCT
    GCCGGCCTGG AGTCCAAGTC CAGCCCCAGC CCCAAAGGGC AGCTCCGAGG GGAGCCGGAG
    CGGAGCAAAC AGCCGCCGCC CGCCTCCTCC CCCACCAAGA GGAGGGGCCG GAGCCGCGTC
    CCCGAGGCTG TGCCCACCTC GCCCGCAGGC GGCCCGCGGC CTACGGCCAA GGACTCCCCT
    CTCAAGAGGG CGCCAGACCC CGTCCCCAGC CCGGCCGCCA AGGAAAGCGG GGAGGAGGCG
    GCCCGAGTCA TCCCCCGGGA GCTCACGGTT AAGAGCAGCT CGCTACTGCC TGAGATCAAG
    CCCGAGCACA AGAGGGGGCC GCTCCCCCAC CTCTTGGATG GCCGGGGAGA CGGAGGTCGA
    AGCAGAGACT TGGGCAGATC GGCTGGAGGT TCTGACGCCT TTGAGGGCTT GAAGCCCAGG
    AACCACTTCG GTGTGGGCAG ATCGACGGTG ACCACGAAAG TGACCCTCCC TGCCAGGCCC
    AAACACGTGG AACTAAATCT TAAAAACTCT AAGAATCTTG ACAGTTTGGG AAGTGAGCAT
    AATCCATTTA GCCAGCCAGT TCACAAAGGA AACACTGCCA CCAAAATCTC CTTATTTGAA
    AACAAGCGGG CAAACAGCAG CCCAAGACAC ACTGACATTC GAGGTTTGAA GAACACTCCT
    GCGTCTAATA AAACATTTGT CGAGAGGGCA AAGCTGAATT TAGCCAAAAA AGCCAAGGAA
    ATGGAGCTGC CTGAAAAGAA GGTGATGCCA AATAGTCACC AGAACGGTGT GCCGGGGAAG
    CTGTCATCTG CAGAAGCCAA AGTGATGCTT CCTGAAGAGG AGACTCCTGT TTCTCAGCTT
    TCCCAAGGTG ATAAGGTAGT TGTGCAAACA GATGCTGGCT GCCCTTCCGA ACCAGTGGTT
    GCTGCTCTGA CTCCCGTTAA GGACCAGGAG ATCTTAGGAG AGAATGACTC CAAGGCTGCT
    AACAGCAAAA GACTTGAACT TGAAAATATG ACTAATATGG CAGAAAACAC CCCTGCCATT
    CTTGATACCA AAGATCCGCC TCCAACAGCC ATACCAAACC CGCAGCCTGG ATTTTCTGAC
    TCACAGCCCC CAGCTGAGTC ATCTAATGGG CCTTCTCTGT CACTGGCTGC CCCCATTCCT
    GCAGATGATA CCAAAGACAC ATGTGCTCAA GCTCCTGTAA GCAGTTCTCT GTGCACTGAT
    CTCAGAGAGT CAGAAAGCCA TAATGGATGT ACTCTGCCTG TATCTCATCA TAATAATGAG
    ACAATGCCCC CCTCCAAACT TGCAGGAGGA AGAGAAGGAG GAGGGGAGAC AAGCCCTCCT
    TTGTCCCCAG AGCACAGCCC GCAAGCTGTG GGAGATGAGT GTCCATCCAA GGTCCTAGTC
    CAGGTCAGGT CCTTCATGCT CCCTGTGGAG AGCATGGAGG GGGTCTGCTC CCAGATCATC
    ACAGAAAGCT CTGAAGTTAG AGAAGCACAG TTGCCAAGCT GTCACAATAA TGAACTCCAA
    GTGGTTTCCG TTGCAAGTGG TGCTCCCCAG AAAGAGGAAG TACAGGGCAA TAAGGGCACA
    TCACCCAAAC ACATTCACTG CAAAGAGGAA CATATAGTAA AATCTGGCGC ACAAGTCATG
    CCGCCTGAAT CAGAAGCAAC TCTGCCTGTC CAGGCTCAAA GTGAGGACAA CGAAAAACCC
    CTGAAGGCTG AATCCACCGC CACCAGCCTC CCTGACAACA GAAGTCATTT AGAAACCCCC
    CAAAGACCCA GTCAGAGTGC TGTGAATGGC CAGGGCAGTC CTGCCAGTCT TTTGAACATC
    TCTGCTGGTA GTGATGATAG TGTATTCGAT TCTTCTTCTG ACATGGAAAA ATTCACTGAG
    ATTATAAAAA AGATGGACAG CACTGTGTGT ATGCCCCAGA AGAAAAAGAA GACCAGGCTA
    CCAAACTCCC CTGCCCCTCA CTTTGCCATG CCACCTATTC ACGAGGACAA TTTAGAAAAG
    GTGTTTGATC CCAACGTGTT TACCTTTGGT TTGGGGAAGA AAAAGGAAGG CCAGTTGGAA
    ATGTCACCAG CTTTACATTT GATGCAGAAT CTTGACACCA AATCCAAACT GAGGCCCAAA
    CGATCATCCA CTGAACAGAG TGTCCTCTTC AAGTCCTTGC AGACTCATAC TAATGGGAAA
    GATGAGCCCC TGGCAGCTCC AGAAGTCAAT GACAAAGAGA ACAAGGATGT CCAAAATGGT
    GGAGTCAAGA GATCGAGGCT AGAAAAAAGT GCACTTTTCT CAAGCTTGTT ATCTTCTCTA
    CCACAAGACA AAATCTTTTC TCCCTCTGTA ACATCAGTCA ATACCATGAC CACATCTTTC
    GGTACTGCTC ATAGCAGTTC TCTTTCTCGA CCTTCCGTGT TGCAGCCTGT GGCTGAGAGT
    GCCCCACCCT GTAGTACAGA GAAAGAACAA CCAAGTCTGC CAGCCAGTAA CTTAAAGGTC
    TTCAATTTCA ACTCGTCAGA TACGTCTCAT TCGGCATTGA AAAGTCTGAG CCCCATGGAA
    AAGTGCCCGC AAAAAGAGGA AACCAAAAAG GATCTGGATC TGCAAAGCAA CCTTCCCATG
    TCAGAAACTA AATTTTCTGA ATTTTCAAAA CTGAAGACCA ACGGTGATAC GTCTAATCAT
    ACTGAAAGTG TTCTTAAATC AAACTTGCCG AGCTATGGAA GCAGTGACAC AGACTTCACT
    GGTCTCTTCA AAGCCAGCCG GTTTGACCCA AGCATTTCTT TTTCTGGAAT GGCATTATCA
    GATACAACAA CAATTAGAGG AAGCGTCCAG AATAAAATCA ATCCCCGACC TGGAAAAGTG
    GTGATATACA GCGAGCCCGA CATCTCAGAG GCATGTATTG AAGTGTTCAG TGACGTGGAG
    GATTGTAGCA CCTGGAGCCT CTCTCCTGTG ATACTAGTAA AAGTCGTTAG AGGATGTTGG
    ATTTTGTATG AGAAACCAAA TTTTGAAGGG CACTCCATCC CCTTAGAAGA AGGAGAATTG
    GAACTCTCTG GTCTCTGGGG TATAGAAGAT ATTTTGGAGA GAAATGAGGA TGAGGCAGTG
    TCGACTAAGC CCATGGTGAT TGGTTCTATC AGACTTGTGG TCCAGGATTA CAGAGTTAGT
    CAGATTGACT TATTTACTGA ACCAGAAGGG TTAGGACTTC TAAATTCCTA CTTTGATGAT
    ACTGAAGAAA TGCAAGGGTT TGGCATAATA CAGAAGACTT GTTCCATTAA AGTACATTGG
    GGCACATGGC TGATTTATGA AGAGCCTGGA TTTCAGGGTG TCCCTTTTAT CCTGGAACCT
    GGTGAATACC CTGACTTATC CTTCTGGGAT ACAGAGGAGG CATACATTGG ATCCATGCGG
    CCACTGAAAA TGGGTGGCCG TAAAGTTGAA TTCCCTACAG ATCCTAAGGT AGTTATTTAT
    GAAAAGCCTT TCTTTGAAGG AAAATGTATG GAGCTGGAAA CAGAGATGGG TAGGTTTATC
    ATGGAAGGAG GTGAAACAGA AGAAACCACT GGAGATGAAC ATTTGTCATT TGTATCAGTG
    GGATCTATGA AAGTTCTACG AGGCATTTGG GTTGCTTATG AGAAGCCTGA CTTTACAGGT
    CATCAGTATT TGCTTGAAGA AGGAGAATAC AAGGACTGGA ATGACTGGGG AGGCTACAGT
    GGAGAACTGC AGTCTTTACG ACCTATATTA GGTGATTTTT CAAATGCTCA CATGATAATG
    TACAGTGAAA AAAACTTTGG ATCCAAAGGT TCCAGTATTG ATGTATTAGG AATTGTTGCT
    AATTTAAAGG AGACTGGATA CGGAGTGAAG ACACAGTCTA TTAATGTACT GAGTGGAGTA
    TGGGTAGCCT ATGAAAATCC TGACTTCACA GGAGAACAGT ACATACTGGA TAAAGGTTTC
    TATACCAGCT TTGAGGACTG GGGAGGCAAA AACTGTAAGA TCTCCTCTGT TCAACCCATA
    TGTTTGGATT CTTTCACTGG CCCAAGGAGA CGAAATCAGA TTCACTTGTT TTCGGAACCA
    CACTTTCAAG GTTGCAGTCA ATGTTTTGGA GAAACAACAA GTCAAATTGA TGATTCCTTC
    TCTACCAAGT CTTGTAGAGT TTTAGGAGGC AGCTGGGTTG CATATGATGG AGAAAATTTC
    TCTGGTAATC AGTACGTGTT GGAGGAAGGC CACTATCCTT CGCTGTCTGC AATGGGATGC
    CACCCTGGAG CAACTTTCAA GTCTCTTCGT TTTATAGATG TTGAATTTTC TGAGCCAACA
    ATTATTCTTT TTGAAAGAGA AGACTTCAAA GGAAAAAAGA TTGAATTTAA TACAGAAATA
    GTTAATCTCC GATCCCTAGG ATTCAACACA CACATACGCT CTGTCCAGGT GATTGGTGGC
    ATATGGGTTA CTTACGAATA TGGCAATTAC AGGGGCCGAC AGTTCCTACT GTCACCAGCG
    GAAGTGCCTA AGTGGTATGA ATTCAGTGGC TGTCACCAAA TAGGTTCTCT ACGACCTTTT
    GTTCAGAAAC GAATTTATTT TAGACTTCGA AACAAAGCAA CAGGGTTATT CATGTCAACC
    AATGGAAACC TAGAGGATCT GAAGCTTCTC AGAATACAGG TCATGGAGGA TGTCGGAGCT
    GATGACCAGA TCTGGATTTA TCAAGAAGGA TGCATCAAAT GCAGGATAGC AGAGGACTGC
    TGCCTGACCA TCTCGGGGAG CCTCGTAACC TCTGGCTCAA AGCTGGGCCT GGCGCTCGAA
    CAGAATGCTG ACAGTCAGTT CTGGAGCATG AAGCCTGACG GCAGGATCTA CAGCAAGCTT
    AAGCCAAATT TAGTTTTAGA TATCAAAGGG GGTACACAGT ATGATCAAAA TCACCTTATC
    CTTAACACTG TCAGCAAAGA GAAGTTAACA CAAGTGTGGG AAGCCATGGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51146
    Clone ID Related Accession (Same CDS sequence) XM_010808463.2 , XM_003582606.4 , XM_010808463.3
    Accession Version XM_010808463.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product absent in melanoma 1 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCCAGAG CACAACCACC AGAAGAGAAC AAAAGGAAGC CAGTTTTGGG AAAACTTGGC 
    ACTCTGTTCA CGGCCGGAAG GAGAAGGAAC AGCAGAAATG GGTTAGAGAG TCCCACCAGC
    TCAAATACCA AACCAGGCTC TCCCAAAGAA GTAACCTCTT CCAGACTCCC GGAAAGAGAG
    ACTGAGAAGA GTCAACCTCT GAGCGGCCAA CCAACGCCAA CAGACGCATG CGAGGAGGGT
    TCCCCACAGG AGAAGTGCCA GGAGCCGGAG GGTGAACACC CCGAGGGCTG CGTGCAGGCA
    GCCCCCCCGG ACGCCAAGCG ATCACCTGGC CGGGGCAGCC ATGCAGCAGC GGCTGTGCAG
    CAGGGCGGTG AAAGTGATTC ACCCCAATTA GAACCTCTGG AGGCAGAGGG AGAGACCTTC
    CCAGATGCCA CCGCCACTGC CGAGCAGCTG CATTCCTCAT TAGAGAACTC CTCCAAGCGG
    GAGAACGCAG AGGCGCTCAT CCGCAGTCCC GGGGAGGACG CTTCGCCCGG TGCTGGCCGC
    GAGCAGGAGC CCACCCAGGG ATCGAGGGGC GTGCCGGGCT CGCCCGCCGG GGAGCATCCC
    GCCGACGGGG CCCCTCGCTT GTGTGCCCAC CGAGGCTCTC CGGAGCCCCA GGGTGGGCTG
    AGCCAAGCCC CGGAGGACTC TTCAGCTCCG CCGGGCGACC CGCTCGCCGA GGGTGCGGAG
    AACCGGGCGG GCTCAGCACC AGTCAACGTC AAAGCCGAGC CCCCGGGGCA AGACTGCCGA
    CCCCGCGAGC GCGCCCACCC AGCCAAAGTG CTGACTCTGG ACATCTACTT AAGTAAGACC
    GAGGCGGCAC AGGTGGACGA GCCGGTCGTG ATCAGCCCGG GGGCGGGAGA TTGCGGCGAT
    TGCGACGACA TGGAAAAGAG GTCGAGCGGC CGCAGGTCTG GGAGAAGGAG GCGGTCTCAG
    AGATCCACCG ACTCCCCCGG CGCTGACCCG CCGTTGCCCG ACTGTGAGCC CCGGGACGAC
    ACGGTGTTCG AGGACGAGGT GGCGCCAGAC GCGGCCGCCG AGAATGGCTG TGCAGAAAAG
    AAAGTGAAAT CTCCTCCGCA GGCGGCCCCC AATGGGGGCG TTGCCTCCGC TGCCGGCCTG
    GAGTCCAAGT CCAGCCCCAG CCCCAAAGGG CAGCTCCGAG GGGAGCCGGA GCGGAGCAAA
    CAGCCGCCGC CCGCCTCCTC CCCCACCAAG AGGAGGGGCC GGAGCCGCGT CCCCGAGGCT
    GTGCCCACCT CGCCCGCAGG CGGCCCGCGG CCTACGGCCA AGGACTCCCC TCTCAAGAGG
    GCGCCAGACC CCGTCCCCAG CCCGGCCGCC AAGGAAAGCG GGGAGGAGGC GGCCCGAGTC
    ATCCCCCGGG AGCTCACGGT TAAGAGCAGC TCGCTACTGC CTGAGATCAA GCCCGAGCAC
    AAGAGGGGGC CGCTCCCCCA CCTCTTGGAT GGCCGGGGAG ACGGAGGTCG AAGCAGAGAC
    TTGGGCAGAT CGGCTGGAGG TTCTGACGCC TTTGAGGGCT TGAAGCCCAG GAACCACTTC
    GGTGTGGGCA GATCGACGGT GACCACGAAA GTGACCCTCC CTGCCAGGCC CAAACACGTG
    GAACTAAATC TTAAAAACTC TAAGAATCTT GACAGTTTGG GAAGTGAGCA TAATCCATTT
    AGCCAGCCAG TTCACAAAGG AAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAGCGG
    GCAAACAGCA GCCCAAGACA CACTGACATT CGAGGTTTGA AGAACACTCC TGCGTCTAAT
    AAAACATTTG TCGAGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAGGA AATGGAGCTG
    CCTGAAAAGA AGGTGATGCC AAATAGTCAC CAGAACGGTG TGCCGGGGAA GCTGTCATCT
    GCAGAAGCCA AAGTGATGCT TCCTGAAGAG GAGACTCCTG TTTCTCAGCT TTCCCAAGGT
    GATAAGGTAG TTGTGCAAAC AGATGCTGGC TGCCCTTCCG AACCAGTGGT TGCTGCTCTG
    ACTCCCGTTA AGGACCAGGA GATCTTAGGA GAGAATGACT CCAAGGCTGC TAACAGCAAA
    AGACTTGAAC TTGAAAATAT GACTAATATG GCAGAAAACA CCCCTGCCAT TCTTGATACC
    AAAGATCCGC CTCCAACAGC CATACCAAAC CCGCAGCCTG GATTTTCTGA CTCACAGCCC
    CCAGCTGAGT CATCTAATGG GCCTTCTCTG TCACTGGCTG CCCCCATTCC TGCAGATGAT
    ACCAAAGACA CATGTGCTCA AGCTCCTGTA AGCAGTTCTC TGTGCACTGA TCTCAGAGAG
    TCAGAAAGCC ATAATGGATG TACTCTGCCT GTATCTCATC ATAATAATGA GACAATGCCC
    CCCTCCAAAC TTGCAGGAGG AAGAGAAGGA GGAGGGGAGA CAAGCCCTCC TTTGTCCCCA
    GAGCACAGCC CGCAAGCTGT GGGAGATGAG TGTCCATCCA AGGTCCTAGT CCAGGTCAGG
    TCCTTCATGC TCCCTGTGGA GAGCATGGAG GGGGTCTGCT CCCAGATCAT CACAGAAAGC
    TCTGAAGTTA GAGAAGCACA GTTGCCAAGC TGTCACAATA ATGAACTCCA AGTGGTTTCC
    GTTGCAAGTG GTGCTCCCCA GAAAGAGGAA GTACAGGGCA ATAAGGGCAC ATCACCCAAA
    CACATTCACT GCAAAGAGGA ACATATAGTA AAATCTGGCG CACAAGTCAT GCCGCCTGAA
    TCAGAAGCAA CTCTGCCTGT CCAGGCTCAA AGTGAGGACA ACGAAAAACC CCTGAAGGCT
    GAATCCACCG CCACCAGCCT CCCTGACAAC AGAAGTCATT TAGAAACCCC CCAAAGACCC
    AGTCAGAGTG CTGTGAATGG CCAGGGCAGT CCTGCCAGTC TTTTGAACAT CTCTGCTGGT
    AGTGATGATA GTGTATTCGA TTCTTCTTCT GACATGGAAA AATTCACTGA GATTATAAAA
    AAGATGGACA GCACTGTGTG TATGCCCCAG AAGAAAAAGA AGACCAGGCT ACCAAACTCC
    CCTGCCCCTC ACTTTGCCAT GCCACCTATT CACGAGGACA ATTTAGAAAA GGTGTTTGAT
    CCCAACGTGT TTACCTTTGG TTTGGGGAAG AAAAAGGAAG GCCAGTTGGA AATGTCACCA
    GCTTTACATT TGATGCAGAA TCTTGACACC AAATCCAAAC TGAGGCCCAA ACGATCATCC
    ACTGAACAGA GTGTCCTCTT CAAGTCCTTG CAGACTCATA CTAATGGGAA AGATGAGCCC
    CTGGCAGCTC CAGAAGTCAA TGACAAAGAG AACAAGGATG TCCAAAATGG TGGAGTCAAG
    AGATCGAGGC TAGAAAAAAG TGCACTTTTC TCAAGCTTGT TATCTTCTCT ACCACAAGAC
    AAAATCTTTT CTCCCTCTGT AACATCAGTC AATACCATGA CCACATCTTT CGGTACTGCT
    CATAGCAGTT CTCTTTCTCG ACCTTCCGTG TTGCAGCCTG TGGCTGAGAG TGCCCCACCC
    TGTAGTACAG AGAAAGAACA ACCAAGTCTG CCAGCCAGTA ACTTAAAGGT CTTCAATTTC
    AACTCGTCAG ATACGTCTCA TTCGGCATTG AAAAGTCTGA GCCCCATGGA AAAGTGCCCG
    CAAAAAGAGG AAACCAAAAA GGATCTGGAT CTGCAAAGCA ACCTTCCCAT GTCAGAAACT
    AAATTTTCTG AATTTTCAAA ACTGAAGACC AACGGTGATA CGTCTAATCA TACTGAAAGT
    GTTCTTAAAT CAAACTTGCC GAGCTATGGA AGCAGTGACA CAGACTTCAC TGGTCTCTTC
    AAAGCCAGCC GGTTTGACCC AAGCATTTCT TTTTCTGGAA TGGCATTATC AGATACAACA
    ACAATTAGAG GAAGCGTCCA GAATAAAATC AATCCCCGAC CTGGAAAAGT GGTGATATAC
    AGCGAGCCCG ACATCTCAGA GGCATGTATT GAAGTGTTCA GTGACGTGGA GGATTGTAGC
    ACCTGGAGCC TCTCTCCTGT GATACTAGTA AAAGTCGTTA GAGGATGTTG GATTTTGTAT
    GAGAAACCAA ATTTTGAAGG GCACTCCATC CCCTTAGAAG AAGGAGAATT GGAACTCTCT
    GGTCTCTGGG GTATAGAAGA TATTTTGGAG AGAAATGAGG ATGAGGCAGT GTCGACTAAG
    CCCATGGTGA TTGGTTCTAT CAGACTTGTG GTCCAGGATT ACAGAGTTAG TCAGATTGAC
    TTATTTACTG AACCAGAAGG GTTAGGACTT CTAAATTCCT ACTTTGATGA TACTGAAGAA
    ATGCAAGGGT TTGGCATAAT ACAGAAGACT TGTTCCATTA AAGTACATTG GGGCACATGG
    CTGATTTATG AAGAGCCTGG ATTTCAGGGT GTCCCTTTTA TCCTGGAACC TGGTGAATAC
    CCTGACTTAT CCTTCTGGGA TACAGAGGAG GCATACATTG GATCCATGCG GCCACTGAAA
    ATGGGTGGCC GTAAAGTTGA ATTCCCTACA GATCCTAAGG TAGTTATTTA TGAAAAGCCT
    TTCTTTGAAG GAAAATGTAT GGAGCTGGAA ACAGAGATGG GTAGGTTTAT CATGGAAGGA
    GGTGAAACAG AAGAAACCAC TGGAGATGAA CATTTGTCAT TTGTATCAGT GGGATCTATG
    AAAGTTCTAC GAGGCATTTG GGTTGCTTAT GAGAAGCCTG ACTTTACAGG TCATCAGTAT
    TTGCTTGAAG AAGGAGAATA CAAGGACTGG AATGACTGGG GAGGCTACAG TGGAGAACTG
    CAGTCTTTAC GACCTATATT AGGTGATTTT TCAAATGCTC ACATGATAAT GTACAGTGAA
    AAAAACTTTG GATCCAAAGG TTCCAGTATT GATGTATTAG GAATTGTTGC TAATTTAAAG
    GAGACTGGAT ACGGAGTGAA GACACAGTCT ATTAATGTAC TGAGTGGAGT ATGGGTAGCC
    TATGAAAATC CTGACTTCAC AGGAGAACAG TACATACTGG ATAAAGGTTT CTATACCAGC
    TTTGAGGACT GGGGAGGCAA AAACTGTAAG ATCTCCTCTG TTCAACCCAT ATGTTTGGAT
    TCTTTCACTG GCCCAAGGAG ACGAAATCAG ATTCACTTGT TTTCGGAACC ACACTTTCAA
    GGTTGCAGTC AATGTTTTGG AGAAACAACA AGTCAAATTG ATGATTCCTT CTCTACCAAG
    TCTTGTAGAG TTTTAGGAGG CAGCTGGGTT GCATATGATG GAGAAAATTT CTCTGGTAAT
    CAGTACGTGT TGGAGGAAGG CCACTATCCT TCGCTGTCTG CAATGGGATG CCACCCTGGA
    GCAACTTTCA AGTCTCTTCG TTTTATAGAT GTTGAATTTT CTGAGCCAAC AATTATTCTT
    TTTGAAAGAG AAGACTTCAA AGGAAAAAAG ATTGAATTTA ATACAGAAAT AGTTAATCTC
    CGATCCCTAG GATTCAACAC ACACATACGC TCTGTCCAGG TGATTGGTGG CATATGGGTT
    ACTTACGAAT ATGGCAATTA CAGGGGCCGA CAGTTCCTAC TGTCACCAGC GGAAGTGCCT
    AAGTGGTATG AATTCAGTGG CTGTCACCAA ATAGGTTCTC TACGACCTTT TGTTCAGAAA
    CGAATTTATT TTAGACTTCG AAACAAAGCA ACAGGGTTAT TCATGTCAAC CAATGGAAAC
    CTAGAGGATC TGAAGCTTCT CAGAATACAG GTCATGGAGG ATGTCGGAGC TGATGACCAG
    ATCTGGATTT ATCAAGAAGG ATGCATCAAA TGCAGGATAG CAGAGGACTG CTGCCTGACC
    ATCTCGGGGA GCCTCGTAAC CTCTGGCTCA AAGCTGGGCC TGGCGCTCGA ACAGAATGCT
    GACAGTCAGT TCTGGAGCAT GAAGCCTGAC GGCAGGATCT ACAGCAAGCT TAAGCCAAAT
    TTAGTTTTAG ATATCAAAGG GGGTACACAG TATGATCAAA ATCACCTTAT CCTTAACACT
    GTCAGCAAAG AGAAGTTAAC ACAAGTGTGG GAAGCCATGG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806765.1
    CDS107..6154
    Translation

    Target ORF information:

    RefSeq Version XM_010808463.2
    Organism Bos taurus(cattle)
    Definition Bos taurus absent in melanoma 1 (AIM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808463.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCCAGAG CACAACCACC AGAAGAGAAC AAAAGGAAGC CAGTTTTGGG AAAACTTGGC 
    ACTCTGTTCA CGGCCGGAAG GAGAAGGAAC AGCAGAAATG GGTTAGAGAG TCCCACCAGC
    TCAAATACCA AACCAGGCTC TCCCAAAGAA GTAACCTCTT CCAGACTCCC GGAAAGAGAG
    ACTGAGAAGA GTCAACCTCT GAGCGGCCAA CCAACGCCAA CAGACGCATG CGAGGAGGGT
    TCCCCACAGG AGAAGTGCCA GGAGCCGGAG GGTGAACACC CCGAGGGCTG CGTGCAGGCA
    GCCCCCCCGG ACGCCAAGCG ATCACCTGGC CGGGGCAGCC ATGCAGCAGC GGCTGTGCAG
    CAGGGCGGTG AAAGTGATTC ACCCCAATTA GAACCTCTGG AGGCAGAGGG AGAGACCTTC
    CCAGATGCCA CCGCCACTGC CGAGCAGCTG CATTCCTCAT TAGAGAACTC CTCCAAGCGG
    GAGAACGCAG AGGCGCTCAT CCGCAGTCCC GGGGAGGACG CTTCGCCCGG TGCTGGCCGC
    GAGCAGGAGC CCACCCAGGG ATCGAGGGGC GTGCCGGGCT CGCCCGCCGG GGAGCATCCC
    GCCGACGGGG CCCCTCGCTT GTGTGCCCAC CGAGGCTCTC CGGAGCCCCA GGGTGGGCTG
    AGCCAAGCCC CGGAGGACTC TTCAGCTCCG CCGGGCGACC CGCTCGCCGA GGGTGCGGAG
    AACCGGGCGG GCTCAGCACC AGTCAACGTC AAAGCCGAGC CCCCGGGGCA AGACTGCCGA
    CCCCGCGAGC GCGCCCACCC AGCCAAAGTG CTGACTCTGG ACATCTACTT AAGTAAGACC
    GAGGCGGCAC AGGTGGACGA GCCGGTCGTG ATCAGCCCGG GGGCGGGAGA TTGCGGCGAT
    TGCGACGACA TGGAAAAGAG GTCGAGCGGC CGCAGGTCTG GGAGAAGGAG GCGGTCTCAG
    AGATCCACCG ACTCCCCCGG CGCTGACCCG CCGTTGCCCG ACTGTGAGCC CCGGGACGAC
    ACGGTGTTCG AGGACGAGGT GGCGCCAGAC GCGGCCGCCG AGAATGGCTG TGCAGAAAAG
    AAAGTGAAAT CTCCTCCGCA GGCGGCCCCC AATGGGGGCG TTGCCTCCGC TGCCGGCCTG
    GAGTCCAAGT CCAGCCCCAG CCCCAAAGGG CAGCTCCGAG GGGAGCCGGA GCGGAGCAAA
    CAGCCGCCGC CCGCCTCCTC CCCCACCAAG AGGAGGGGCC GGAGCCGCGT CCCCGAGGCT
    GTGCCCACCT CGCCCGCAGG CGGCCCGCGG CCTACGGCCA AGGACTCCCC TCTCAAGAGG
    GCGCCAGACC CCGTCCCCAG CCCGGCCGCC AAGGAAAGCG GGGAGGAGGC GGCCCGAGTC
    ATCCCCCGGG AGCTCACGGT TAAGAGCAGC TCGCTACTGC CTGAGATCAA GCCCGAGCAC
    AAGAGGGGGC CGCTCCCCCA CCTCTTGGAT GGCCGGGGAG ACGGAGGTCG AAGCAGAGAC
    TTGGGCAGAT CGGCTGGAGG TTCTGACGCC TTTGAGGGCT TGAAGCCCAG GAACCACTTC
    GGTGTGGGCA GATCGACGGT GACCACGAAA GTGACCCTCC CTGCCAGGCC CAAACACGTG
    GAACTAAATC TTAAAAACTC TAAGAATCTT GACAGTTTGG GAAGTGAGCA TAATCCATTT
    AGCCAGCCAG TTCACAAAGG AAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAGCGG
    GCAAACAGCA GCCCAAGACA CACTGACATT CGAGGTTTGA AGAACACTCC TGCGTCTAAT
    AAAACATTTG TCGAGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAGGA AATGGAGCTG
    CCTGAAAAGA AGGTGATGCC AAATAGTCAC CAGAACGGTG TGCCGGGGAA GCTGTCATCT
    GCAGAAGCCA AAGTGATGCT TCCTGAAGAG GAGACTCCTG TTTCTCAGCT TTCCCAAGGT
    GATAAGGTAG TTGTGCAAAC AGATGCTGGC TGCCCTTCCG AACCAGTGGT TGCTGCTCTG
    ACTCCCGTTA AGGACCAGGA GATCTTAGGA GAGAATGACT CCAAGGCTGC TAACAGCAAA
    AGACTTGAAC TTGAAAATAT GACTAATATG GCAGAAAACA CCCCTGCCAT TCTTGATACC
    AAAGATCCGC CTCCAACAGC CATACCAAAC CCGCAGCCTG GATTTTCTGA CTCACAGCCC
    CCAGCTGAGT CATCTAATGG GCCTTCTCTG TCACTGGCTG CCCCCATTCC TGCAGATGAT
    ACCAAAGACA CATGTGCTCA AGCTCCTGTA AGCAGTTCTC TGTGCACTGA TCTCAGAGAG
    TCAGAAAGCC ATAATGGATG TACTCTGCCT GTATCTCATC ATAATAATGA GACAATGCCC
    CCCTCCAAAC TTGCAGGAGG AAGAGAAGGA GGAGGGGAGA CAAGCCCTCC TTTGTCCCCA
    GAGCACAGCC CGCAAGCTGT GGGAGATGAG TGTCCATCCA AGGTCCTAGT CCAGGTCAGG
    TCCTTCATGC TCCCTGTGGA GAGCATGGAG GGGGTCTGCT CCCAGATCAT CACAGAAAGC
    TCTGAAGTTA GAGAAGCACA GTTGCCAAGC TGTCACAATA ATGAACTCCA AGTGGTTTCC
    GTTGCAAGTG GTGCTCCCCA GAAAGAGGAA GTACAGGGCA ATAAGGGCAC ATCACCCAAA
    CACATTCACT GCAAAGAGGA ACATATAGTA AAATCTGGCG CACAAGTCAT GCCGCCTGAA
    TCAGAAGCAA CTCTGCCTGT CCAGGCTCAA AGTGAGGACA ACGAAAAACC CCTGAAGGCT
    GAATCCACCG CCACCAGCCT CCCTGACAAC AGAAGTCATT TAGAAACCCC CCAAAGACCC
    AGTCAGAGTG CTGTGAATGG CCAGGGCAGT CCTGCCAGTC TTTTGAACAT CTCTGCTGGT
    AGTGATGATA GTGTATTCGA TTCTTCTTCT GACATGGAAA AATTCACTGA GATTATAAAA
    AAGATGGACA GCACTGTGTG TATGCCCCAG AAGAAAAAGA AGACCAGGCT ACCAAACTCC
    CCTGCCCCTC ACTTTGCCAT GCCACCTATT CACGAGGACA ATTTAGAAAA GGTGTTTGAT
    CCCAACGTGT TTACCTTTGG TTTGGGGAAG AAAAAGGAAG GCCAGTTGGA AATGTCACCA
    GCTTTACATT TGATGCAGAA TCTTGACACC AAATCCAAAC TGAGGCCCAA ACGATCATCC
    ACTGAACAGA GTGTCCTCTT CAAGTCCTTG CAGACTCATA CTAATGGGAA AGATGAGCCC
    CTGGCAGCTC CAGAAGTCAA TGACAAAGAG AACAAGGATG TCCAAAATGG TGGAGTCAAG
    AGATCGAGGC TAGAAAAAAG TGCACTTTTC TCAAGCTTGT TATCTTCTCT ACCACAAGAC
    AAAATCTTTT CTCCCTCTGT AACATCAGTC AATACCATGA CCACATCTTT CGGTACTGCT
    CATAGCAGTT CTCTTTCTCG ACCTTCCGTG TTGCAGCCTG TGGCTGAGAG TGCCCCACCC
    TGTAGTACAG AGAAAGAACA ACCAAGTCTG CCAGCCAGTA ACTTAAAGGT CTTCAATTTC
    AACTCGTCAG ATACGTCTCA TTCGGCATTG AAAAGTCTGA GCCCCATGGA AAAGTGCCCG
    CAAAAAGAGG AAACCAAAAA GGATCTGGAT CTGCAAAGCA ACCTTCCCAT GTCAGAAACT
    AAATTTTCTG AATTTTCAAA ACTGAAGACC AACGGTGATA CGTCTAATCA TACTGAAAGT
    GTTCTTAAAT CAAACTTGCC GAGCTATGGA AGCAGTGACA CAGACTTCAC TGGTCTCTTC
    AAAGCCAGCC GGTTTGACCC AAGCATTTCT TTTTCTGGAA TGGCATTATC AGATACAACA
    ACAATTAGAG GAAGCGTCCA GAATAAAATC AATCCCCGAC CTGGAAAAGT GGTGATATAC
    AGCGAGCCCG ACATCTCAGA GGCATGTATT GAAGTGTTCA GTGACGTGGA GGATTGTAGC
    ACCTGGAGCC TCTCTCCTGT GATACTAGTA AAAGTCGTTA GAGGATGTTG GATTTTGTAT
    GAGAAACCAA ATTTTGAAGG GCACTCCATC CCCTTAGAAG AAGGAGAATT GGAACTCTCT
    GGTCTCTGGG GTATAGAAGA TATTTTGGAG AGAAATGAGG ATGAGGCAGT GTCGACTAAG
    CCCATGGTGA TTGGTTCTAT CAGACTTGTG GTCCAGGATT ACAGAGTTAG TCAGATTGAC
    TTATTTACTG AACCAGAAGG GTTAGGACTT CTAAATTCCT ACTTTGATGA TACTGAAGAA
    ATGCAAGGGT TTGGCATAAT ACAGAAGACT TGTTCCATTA AAGTACATTG GGGCACATGG
    CTGATTTATG AAGAGCCTGG ATTTCAGGGT GTCCCTTTTA TCCTGGAACC TGGTGAATAC
    CCTGACTTAT CCTTCTGGGA TACAGAGGAG GCATACATTG GATCCATGCG GCCACTGAAA
    ATGGGTGGCC GTAAAGTTGA ATTCCCTACA GATCCTAAGG TAGTTATTTA TGAAAAGCCT
    TTCTTTGAAG GAAAATGTAT GGAGCTGGAA ACAGAGATGG GTAGGTTTAT CATGGAAGGA
    GGTGAAACAG AAGAAACCAC TGGAGATGAA CATTTGTCAT TTGTATCAGT GGGATCTATG
    AAAGTTCTAC GAGGCATTTG GGTTGCTTAT GAGAAGCCTG ACTTTACAGG TCATCAGTAT
    TTGCTTGAAG AAGGAGAATA CAAGGACTGG AATGACTGGG GAGGCTACAG TGGAGAACTG
    CAGTCTTTAC GACCTATATT AGGTGATTTT TCAAATGCTC ACATGATAAT GTACAGTGAA
    AAAAACTTTG GATCCAAAGG TTCCAGTATT GATGTATTAG GAATTGTTGC TAATTTAAAG
    GAGACTGGAT ACGGAGTGAA GACACAGTCT ATTAATGTAC TGAGTGGAGT ATGGGTAGCC
    TATGAAAATC CTGACTTCAC AGGAGAACAG TACATACTGG ATAAAGGTTT CTATACCAGC
    TTTGAGGACT GGGGAGGCAA AAACTGTAAG ATCTCCTCTG TTCAACCCAT ATGTTTGGAT
    TCTTTCACTG GCCCAAGGAG ACGAAATCAG ATTCACTTGT TTTCGGAACC ACACTTTCAA
    GGTTGCAGTC AATGTTTTGG AGAAACAACA AGTCAAATTG ATGATTCCTT CTCTACCAAG
    TCTTGTAGAG TTTTAGGAGG CAGCTGGGTT GCATATGATG GAGAAAATTT CTCTGGTAAT
    CAGTACGTGT TGGAGGAAGG CCACTATCCT TCGCTGTCTG CAATGGGATG CCACCCTGGA
    GCAACTTTCA AGTCTCTTCG TTTTATAGAT GTTGAATTTT CTGAGCCAAC AATTATTCTT
    TTTGAAAGAG AAGACTTCAA AGGAAAAAAG ATTGAATTTA ATACAGAAAT AGTTAATCTC
    CGATCCCTAG GATTCAACAC ACACATACGC TCTGTCCAGG TGATTGGTGG CATATGGGTT
    ACTTACGAAT ATGGCAATTA CAGGGGCCGA CAGTTCCTAC TGTCACCAGC GGAAGTGCCT
    AAGTGGTATG AATTCAGTGG CTGTCACCAA ATAGGTTCTC TACGACCTTT TGTTCAGAAA
    CGAATTTATT TTAGACTTCG AAACAAAGCA ACAGGGTTAT TCATGTCAAC CAATGGAAAC
    CTAGAGGATC TGAAGCTTCT CAGAATACAG GTCATGGAGG ATGTCGGAGC TGATGACCAG
    ATCTGGATTT ATCAAGAAGG ATGCATCAAA TGCAGGATAG CAGAGGACTG CTGCCTGACC
    ATCTCGGGGA GCCTCGTAAC CTCTGGCTCA AAGCTGGGCC TGGCGCTCGA ACAGAATGCT
    GACAGTCAGT TCTGGAGCAT GAAGCCTGAC GGCAGGATCT ACAGCAAGCT TAAGCCAAAT
    TTAGTTTTAG ATATCAAAGG GGGTACACAG TATGATCAAA ATCACCTTAT CCTTAACACT
    GTCAGCAAAG AGAAGTTAAC ACAAGTGTGG GAAGCCATGG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51146
    Clone ID Related Accession (Same CDS sequence) XM_010808463.2 , XM_003582606.4 , XM_010808463.3
    Accession Version XM_003582606.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product absent in melanoma 1 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582606.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCCAGAG CACAACCACC AGAAGAGAAC AAAAGGAAGC CAGTTTTGGG AAAACTTGGC 
    ACTCTGTTCA CGGCCGGAAG GAGAAGGAAC AGCAGAAATG GGTTAGAGAG TCCCACCAGC
    TCAAATACCA AACCAGGCTC TCCCAAAGAA GTAACCTCTT CCAGACTCCC GGAAAGAGAG
    ACTGAGAAGA GTCAACCTCT GAGCGGCCAA CCAACGCCAA CAGACGCATG CGAGGAGGGT
    TCCCCACAGG AGAAGTGCCA GGAGCCGGAG GGTGAACACC CCGAGGGCTG CGTGCAGGCA
    GCCCCCCCGG ACGCCAAGCG ATCACCTGGC CGGGGCAGCC ATGCAGCAGC GGCTGTGCAG
    CAGGGCGGTG AAAGTGATTC ACCCCAATTA GAACCTCTGG AGGCAGAGGG AGAGACCTTC
    CCAGATGCCA CCGCCACTGC CGAGCAGCTG CATTCCTCAT TAGAGAACTC CTCCAAGCGG
    GAGAACGCAG AGGCGCTCAT CCGCAGTCCC GGGGAGGACG CTTCGCCCGG TGCTGGCCGC
    GAGCAGGAGC CCACCCAGGG ATCGAGGGGC GTGCCGGGCT CGCCCGCCGG GGAGCATCCC
    GCCGACGGGG CCCCTCGCTT GTGTGCCCAC CGAGGCTCTC CGGAGCCCCA GGGTGGGCTG
    AGCCAAGCCC CGGAGGACTC TTCAGCTCCG CCGGGCGACC CGCTCGCCGA GGGTGCGGAG
    AACCGGGCGG GCTCAGCACC AGTCAACGTC AAAGCCGAGC CCCCGGGGCA AGACTGCCGA
    CCCCGCGAGC GCGCCCACCC AGCCAAAGTG CTGACTCTGG ACATCTACTT AAGTAAGACC
    GAGGCGGCAC AGGTGGACGA GCCGGTCGTG ATCAGCCCGG GGGCGGGAGA TTGCGGCGAT
    TGCGACGACA TGGAAAAGAG GTCGAGCGGC CGCAGGTCTG GGAGAAGGAG GCGGTCTCAG
    AGATCCACCG ACTCCCCCGG CGCTGACCCG CCGTTGCCCG ACTGTGAGCC CCGGGACGAC
    ACGGTGTTCG AGGACGAGGT GGCGCCAGAC GCGGCCGCCG AGAATGGCTG TGCAGAAAAG
    AAAGTGAAAT CTCCTCCGCA GGCGGCCCCC AATGGGGGCG TTGCCTCCGC TGCCGGCCTG
    GAGTCCAAGT CCAGCCCCAG CCCCAAAGGG CAGCTCCGAG GGGAGCCGGA GCGGAGCAAA
    CAGCCGCCGC CCGCCTCCTC CCCCACCAAG AGGAGGGGCC GGAGCCGCGT CCCCGAGGCT
    GTGCCCACCT CGCCCGCAGG CGGCCCGCGG CCTACGGCCA AGGACTCCCC TCTCAAGAGG
    GCGCCAGACC CCGTCCCCAG CCCGGCCGCC AAGGAAAGCG GGGAGGAGGC GGCCCGAGTC
    ATCCCCCGGG AGCTCACGGT TAAGAGCAGC TCGCTACTGC CTGAGATCAA GCCCGAGCAC
    AAGAGGGGGC CGCTCCCCCA CCTCTTGGAT GGCCGGGGAG ACGGAGGTCG AAGCAGAGAC
    TTGGGCAGAT CGGCTGGAGG TTCTGACGCC TTTGAGGGCT TGAAGCCCAG GAACCACTTC
    GGTGTGGGCA GATCGACGGT GACCACGAAA GTGACCCTCC CTGCCAGGCC CAAACACGTG
    GAACTAAATC TTAAAAACTC TAAGAATCTT GACAGTTTGG GAAGTGAGCA TAATCCATTT
    AGCCAGCCAG TTCACAAAGG AAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAGCGG
    GCAAACAGCA GCCCAAGACA CACTGACATT CGAGGTTTGA AGAACACTCC TGCGTCTAAT
    AAAACATTTG TCGAGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAGGA AATGGAGCTG
    CCTGAAAAGA AGGTGATGCC AAATAGTCAC CAGAACGGTG TGCCGGGGAA GCTGTCATCT
    GCAGAAGCCA AAGTGATGCT TCCTGAAGAG GAGACTCCTG TTTCTCAGCT TTCCCAAGGT
    GATAAGGTAG TTGTGCAAAC AGATGCTGGC TGCCCTTCCG AACCAGTGGT TGCTGCTCTG
    ACTCCCGTTA AGGACCAGGA GATCTTAGGA GAGAATGACT CCAAGGCTGC TAACAGCAAA
    AGACTTGAAC TTGAAAATAT GACTAATATG GCAGAAAACA CCCCTGCCAT TCTTGATACC
    AAAGATCCGC CTCCAACAGC CATACCAAAC CCGCAGCCTG GATTTTCTGA CTCACAGCCC
    CCAGCTGAGT CATCTAATGG GCCTTCTCTG TCACTGGCTG CCCCCATTCC TGCAGATGAT
    ACCAAAGACA CATGTGCTCA AGCTCCTGTA AGCAGTTCTC TGTGCACTGA TCTCAGAGAG
    TCAGAAAGCC ATAATGGATG TACTCTGCCT GTATCTCATC ATAATAATGA GACAATGCCC
    CCCTCCAAAC TTGCAGGAGG AAGAGAAGGA GGAGGGGAGA CAAGCCCTCC TTTGTCCCCA
    GAGCACAGCC CGCAAGCTGT GGGAGATGAG TGTCCATCCA AGGTCCTAGT CCAGGTCAGG
    TCCTTCATGC TCCCTGTGGA GAGCATGGAG GGGGTCTGCT CCCAGATCAT CACAGAAAGC
    TCTGAAGTTA GAGAAGCACA GTTGCCAAGC TGTCACAATA ATGAACTCCA AGTGGTTTCC
    GTTGCAAGTG GTGCTCCCCA GAAAGAGGAA GTACAGGGCA ATAAGGGCAC ATCACCCAAA
    CACATTCACT GCAAAGAGGA ACATATAGTA AAATCTGGCG CACAAGTCAT GCCGCCTGAA
    TCAGAAGCAA CTCTGCCTGT CCAGGCTCAA AGTGAGGACA ACGAAAAACC CCTGAAGGCT
    GAATCCACCG CCACCAGCCT CCCTGACAAC AGAAGTCATT TAGAAACCCC CCAAAGACCC
    AGTCAGAGTG CTGTGAATGG CCAGGGCAGT CCTGCCAGTC TTTTGAACAT CTCTGCTGGT
    AGTGATGATA GTGTATTCGA TTCTTCTTCT GACATGGAAA AATTCACTGA GATTATAAAA
    AAGATGGACA GCACTGTGTG TATGCCCCAG AAGAAAAAGA AGACCAGGCT ACCAAACTCC
    CCTGCCCCTC ACTTTGCCAT GCCACCTATT CACGAGGACA ATTTAGAAAA GGTGTTTGAT
    CCCAACGTGT TTACCTTTGG TTTGGGGAAG AAAAAGGAAG GCCAGTTGGA AATGTCACCA
    GCTTTACATT TGATGCAGAA TCTTGACACC AAATCCAAAC TGAGGCCCAA ACGATCATCC
    ACTGAACAGA GTGTCCTCTT CAAGTCCTTG CAGACTCATA CTAATGGGAA AGATGAGCCC
    CTGGCAGCTC CAGAAGTCAA TGACAAAGAG AACAAGGATG TCCAAAATGG TGGAGTCAAG
    AGATCGAGGC TAGAAAAAAG TGCACTTTTC TCAAGCTTGT TATCTTCTCT ACCACAAGAC
    AAAATCTTTT CTCCCTCTGT AACATCAGTC AATACCATGA CCACATCTTT CGGTACTGCT
    CATAGCAGTT CTCTTTCTCG ACCTTCCGTG TTGCAGCCTG TGGCTGAGAG TGCCCCACCC
    TGTAGTACAG AGAAAGAACA ACCAAGTCTG CCAGCCAGTA ACTTAAAGGT CTTCAATTTC
    AACTCGTCAG ATACGTCTCA TTCGGCATTG AAAAGTCTGA GCCCCATGGA AAAGTGCCCG
    CAAAAAGAGG AAACCAAAAA GGATCTGGAT CTGCAAAGCA ACCTTCCCAT GTCAGAAACT
    AAATTTTCTG AATTTTCAAA ACTGAAGACC AACGGTGATA CGTCTAATCA TACTGAAAGT
    GTTCTTAAAT CAAACTTGCC GAGCTATGGA AGCAGTGACA CAGACTTCAC TGGTCTCTTC
    AAAGCCAGCC GGTTTGACCC AAGCATTTCT TTTTCTGGAA TGGCATTATC AGATACAACA
    ACAATTAGAG GAAGCGTCCA GAATAAAATC AATCCCCGAC CTGGAAAAGT GGTGATATAC
    AGCGAGCCCG ACATCTCAGA GGCATGTATT GAAGTGTTCA GTGACGTGGA GGATTGTAGC
    ACCTGGAGCC TCTCTCCTGT GATACTAGTA AAAGTCGTTA GAGGATGTTG GATTTTGTAT
    GAGAAACCAA ATTTTGAAGG GCACTCCATC CCCTTAGAAG AAGGAGAATT GGAACTCTCT
    GGTCTCTGGG GTATAGAAGA TATTTTGGAG AGAAATGAGG ATGAGGCAGT GTCGACTAAG
    CCCATGGTGA TTGGTTCTAT CAGACTTGTG GTCCAGGATT ACAGAGTTAG TCAGATTGAC
    TTATTTACTG AACCAGAAGG GTTAGGACTT CTAAATTCCT ACTTTGATGA TACTGAAGAA
    ATGCAAGGGT TTGGCATAAT ACAGAAGACT TGTTCCATTA AAGTACATTG GGGCACATGG
    CTGATTTATG AAGAGCCTGG ATTTCAGGGT GTCCCTTTTA TCCTGGAACC TGGTGAATAC
    CCTGACTTAT CCTTCTGGGA TACAGAGGAG GCATACATTG GATCCATGCG GCCACTGAAA
    ATGGGTGGCC GTAAAGTTGA ATTCCCTACA GATCCTAAGG TAGTTATTTA TGAAAAGCCT
    TTCTTTGAAG GAAAATGTAT GGAGCTGGAA ACAGAGATGG GTAGGTTTAT CATGGAAGGA
    GGTGAAACAG AAGAAACCAC TGGAGATGAA CATTTGTCAT TTGTATCAGT GGGATCTATG
    AAAGTTCTAC GAGGCATTTG GGTTGCTTAT GAGAAGCCTG ACTTTACAGG TCATCAGTAT
    TTGCTTGAAG AAGGAGAATA CAAGGACTGG AATGACTGGG GAGGCTACAG TGGAGAACTG
    CAGTCTTTAC GACCTATATT AGGTGATTTT TCAAATGCTC ACATGATAAT GTACAGTGAA
    AAAAACTTTG GATCCAAAGG TTCCAGTATT GATGTATTAG GAATTGTTGC TAATTTAAAG
    GAGACTGGAT ACGGAGTGAA GACACAGTCT ATTAATGTAC TGAGTGGAGT ATGGGTAGCC
    TATGAAAATC CTGACTTCAC AGGAGAACAG TACATACTGG ATAAAGGTTT CTATACCAGC
    TTTGAGGACT GGGGAGGCAA AAACTGTAAG ATCTCCTCTG TTCAACCCAT ATGTTTGGAT
    TCTTTCACTG GCCCAAGGAG ACGAAATCAG ATTCACTTGT TTTCGGAACC ACACTTTCAA
    GGTTGCAGTC AATGTTTTGG AGAAACAACA AGTCAAATTG ATGATTCCTT CTCTACCAAG
    TCTTGTAGAG TTTTAGGAGG CAGCTGGGTT GCATATGATG GAGAAAATTT CTCTGGTAAT
    CAGTACGTGT TGGAGGAAGG CCACTATCCT TCGCTGTCTG CAATGGGATG CCACCCTGGA
    GCAACTTTCA AGTCTCTTCG TTTTATAGAT GTTGAATTTT CTGAGCCAAC AATTATTCTT
    TTTGAAAGAG AAGACTTCAA AGGAAAAAAG ATTGAATTTA ATACAGAAAT AGTTAATCTC
    CGATCCCTAG GATTCAACAC ACACATACGC TCTGTCCAGG TGATTGGTGG CATATGGGTT
    ACTTACGAAT ATGGCAATTA CAGGGGCCGA CAGTTCCTAC TGTCACCAGC GGAAGTGCCT
    AAGTGGTATG AATTCAGTGG CTGTCACCAA ATAGGTTCTC TACGACCTTT TGTTCAGAAA
    CGAATTTATT TTAGACTTCG AAACAAAGCA ACAGGGTTAT TCATGTCAAC CAATGGAAAC
    CTAGAGGATC TGAAGCTTCT CAGAATACAG GTCATGGAGG ATGTCGGAGC TGATGACCAG
    ATCTGGATTT ATCAAGAAGG ATGCATCAAA TGCAGGATAG CAGAGGACTG CTGCCTGACC
    ATCTCGGGGA GCCTCGTAAC CTCTGGCTCA AAGCTGGGCC TGGCGCTCGA ACAGAATGCT
    GACAGTCAGT TCTGGAGCAT GAAGCCTGAC GGCAGGATCT ACAGCAAGCT TAAGCCAAAT
    TTAGTTTTAG ATATCAAAGG GGGTACACAG TATGATCAAA ATCACCTTAT CCTTAACACT
    GTCAGCAAAG AGAAGTTAAC ACAAGTGTGG GAAGCCATGG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582654.3
    CDS107..6154
    Translation

    Target ORF information:

    RefSeq Version XM_003582606.4
    Organism Bos taurus(cattle)
    Definition Bos taurus absent in melanoma 1 (AIM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582606.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCCAGAG CACAACCACC AGAAGAGAAC AAAAGGAAGC CAGTTTTGGG AAAACTTGGC 
    ACTCTGTTCA CGGCCGGAAG GAGAAGGAAC AGCAGAAATG GGTTAGAGAG TCCCACCAGC
    TCAAATACCA AACCAGGCTC TCCCAAAGAA GTAACCTCTT CCAGACTCCC GGAAAGAGAG
    ACTGAGAAGA GTCAACCTCT GAGCGGCCAA CCAACGCCAA CAGACGCATG CGAGGAGGGT
    TCCCCACAGG AGAAGTGCCA GGAGCCGGAG GGTGAACACC CCGAGGGCTG CGTGCAGGCA
    GCCCCCCCGG ACGCCAAGCG ATCACCTGGC CGGGGCAGCC ATGCAGCAGC GGCTGTGCAG
    CAGGGCGGTG AAAGTGATTC ACCCCAATTA GAACCTCTGG AGGCAGAGGG AGAGACCTTC
    CCAGATGCCA CCGCCACTGC CGAGCAGCTG CATTCCTCAT TAGAGAACTC CTCCAAGCGG
    GAGAACGCAG AGGCGCTCAT CCGCAGTCCC GGGGAGGACG CTTCGCCCGG TGCTGGCCGC
    GAGCAGGAGC CCACCCAGGG ATCGAGGGGC GTGCCGGGCT CGCCCGCCGG GGAGCATCCC
    GCCGACGGGG CCCCTCGCTT GTGTGCCCAC CGAGGCTCTC CGGAGCCCCA GGGTGGGCTG
    AGCCAAGCCC CGGAGGACTC TTCAGCTCCG CCGGGCGACC CGCTCGCCGA GGGTGCGGAG
    AACCGGGCGG GCTCAGCACC AGTCAACGTC AAAGCCGAGC CCCCGGGGCA AGACTGCCGA
    CCCCGCGAGC GCGCCCACCC AGCCAAAGTG CTGACTCTGG ACATCTACTT AAGTAAGACC
    GAGGCGGCAC AGGTGGACGA GCCGGTCGTG ATCAGCCCGG GGGCGGGAGA TTGCGGCGAT
    TGCGACGACA TGGAAAAGAG GTCGAGCGGC CGCAGGTCTG GGAGAAGGAG GCGGTCTCAG
    AGATCCACCG ACTCCCCCGG CGCTGACCCG CCGTTGCCCG ACTGTGAGCC CCGGGACGAC
    ACGGTGTTCG AGGACGAGGT GGCGCCAGAC GCGGCCGCCG AGAATGGCTG TGCAGAAAAG
    AAAGTGAAAT CTCCTCCGCA GGCGGCCCCC AATGGGGGCG TTGCCTCCGC TGCCGGCCTG
    GAGTCCAAGT CCAGCCCCAG CCCCAAAGGG CAGCTCCGAG GGGAGCCGGA GCGGAGCAAA
    CAGCCGCCGC CCGCCTCCTC CCCCACCAAG AGGAGGGGCC GGAGCCGCGT CCCCGAGGCT
    GTGCCCACCT CGCCCGCAGG CGGCCCGCGG CCTACGGCCA AGGACTCCCC TCTCAAGAGG
    GCGCCAGACC CCGTCCCCAG CCCGGCCGCC AAGGAAAGCG GGGAGGAGGC GGCCCGAGTC
    ATCCCCCGGG AGCTCACGGT TAAGAGCAGC TCGCTACTGC CTGAGATCAA GCCCGAGCAC
    AAGAGGGGGC CGCTCCCCCA CCTCTTGGAT GGCCGGGGAG ACGGAGGTCG AAGCAGAGAC
    TTGGGCAGAT CGGCTGGAGG TTCTGACGCC TTTGAGGGCT TGAAGCCCAG GAACCACTTC
    GGTGTGGGCA GATCGACGGT GACCACGAAA GTGACCCTCC CTGCCAGGCC CAAACACGTG
    GAACTAAATC TTAAAAACTC TAAGAATCTT GACAGTTTGG GAAGTGAGCA TAATCCATTT
    AGCCAGCCAG TTCACAAAGG AAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAGCGG
    GCAAACAGCA GCCCAAGACA CACTGACATT CGAGGTTTGA AGAACACTCC TGCGTCTAAT
    AAAACATTTG TCGAGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAGGA AATGGAGCTG
    CCTGAAAAGA AGGTGATGCC AAATAGTCAC CAGAACGGTG TGCCGGGGAA GCTGTCATCT
    GCAGAAGCCA AAGTGATGCT TCCTGAAGAG GAGACTCCTG TTTCTCAGCT TTCCCAAGGT
    GATAAGGTAG TTGTGCAAAC AGATGCTGGC TGCCCTTCCG AACCAGTGGT TGCTGCTCTG
    ACTCCCGTTA AGGACCAGGA GATCTTAGGA GAGAATGACT CCAAGGCTGC TAACAGCAAA
    AGACTTGAAC TTGAAAATAT GACTAATATG GCAGAAAACA CCCCTGCCAT TCTTGATACC
    AAAGATCCGC CTCCAACAGC CATACCAAAC CCGCAGCCTG GATTTTCTGA CTCACAGCCC
    CCAGCTGAGT CATCTAATGG GCCTTCTCTG TCACTGGCTG CCCCCATTCC TGCAGATGAT
    ACCAAAGACA CATGTGCTCA AGCTCCTGTA AGCAGTTCTC TGTGCACTGA TCTCAGAGAG
    TCAGAAAGCC ATAATGGATG TACTCTGCCT GTATCTCATC ATAATAATGA GACAATGCCC
    CCCTCCAAAC TTGCAGGAGG AAGAGAAGGA GGAGGGGAGA CAAGCCCTCC TTTGTCCCCA
    GAGCACAGCC CGCAAGCTGT GGGAGATGAG TGTCCATCCA AGGTCCTAGT CCAGGTCAGG
    TCCTTCATGC TCCCTGTGGA GAGCATGGAG GGGGTCTGCT CCCAGATCAT CACAGAAAGC
    TCTGAAGTTA GAGAAGCACA GTTGCCAAGC TGTCACAATA ATGAACTCCA AGTGGTTTCC
    GTTGCAAGTG GTGCTCCCCA GAAAGAGGAA GTACAGGGCA ATAAGGGCAC ATCACCCAAA
    CACATTCACT GCAAAGAGGA ACATATAGTA AAATCTGGCG CACAAGTCAT GCCGCCTGAA
    TCAGAAGCAA CTCTGCCTGT CCAGGCTCAA AGTGAGGACA ACGAAAAACC CCTGAAGGCT
    GAATCCACCG CCACCAGCCT CCCTGACAAC AGAAGTCATT TAGAAACCCC CCAAAGACCC
    AGTCAGAGTG CTGTGAATGG CCAGGGCAGT CCTGCCAGTC TTTTGAACAT CTCTGCTGGT
    AGTGATGATA GTGTATTCGA TTCTTCTTCT GACATGGAAA AATTCACTGA GATTATAAAA
    AAGATGGACA GCACTGTGTG TATGCCCCAG AAGAAAAAGA AGACCAGGCT ACCAAACTCC
    CCTGCCCCTC ACTTTGCCAT GCCACCTATT CACGAGGACA ATTTAGAAAA GGTGTTTGAT
    CCCAACGTGT TTACCTTTGG TTTGGGGAAG AAAAAGGAAG GCCAGTTGGA AATGTCACCA
    GCTTTACATT TGATGCAGAA TCTTGACACC AAATCCAAAC TGAGGCCCAA ACGATCATCC
    ACTGAACAGA GTGTCCTCTT CAAGTCCTTG CAGACTCATA CTAATGGGAA AGATGAGCCC
    CTGGCAGCTC CAGAAGTCAA TGACAAAGAG AACAAGGATG TCCAAAATGG TGGAGTCAAG
    AGATCGAGGC TAGAAAAAAG TGCACTTTTC TCAAGCTTGT TATCTTCTCT ACCACAAGAC
    AAAATCTTTT CTCCCTCTGT AACATCAGTC AATACCATGA CCACATCTTT CGGTACTGCT
    CATAGCAGTT CTCTTTCTCG ACCTTCCGTG TTGCAGCCTG TGGCTGAGAG TGCCCCACCC
    TGTAGTACAG AGAAAGAACA ACCAAGTCTG CCAGCCAGTA ACTTAAAGGT CTTCAATTTC
    AACTCGTCAG ATACGTCTCA TTCGGCATTG AAAAGTCTGA GCCCCATGGA AAAGTGCCCG
    CAAAAAGAGG AAACCAAAAA GGATCTGGAT CTGCAAAGCA ACCTTCCCAT GTCAGAAACT
    AAATTTTCTG AATTTTCAAA ACTGAAGACC AACGGTGATA CGTCTAATCA TACTGAAAGT
    GTTCTTAAAT CAAACTTGCC GAGCTATGGA AGCAGTGACA CAGACTTCAC TGGTCTCTTC
    AAAGCCAGCC GGTTTGACCC AAGCATTTCT TTTTCTGGAA TGGCATTATC AGATACAACA
    ACAATTAGAG GAAGCGTCCA GAATAAAATC AATCCCCGAC CTGGAAAAGT GGTGATATAC
    AGCGAGCCCG ACATCTCAGA GGCATGTATT GAAGTGTTCA GTGACGTGGA GGATTGTAGC
    ACCTGGAGCC TCTCTCCTGT GATACTAGTA AAAGTCGTTA GAGGATGTTG GATTTTGTAT
    GAGAAACCAA ATTTTGAAGG GCACTCCATC CCCTTAGAAG AAGGAGAATT GGAACTCTCT
    GGTCTCTGGG GTATAGAAGA TATTTTGGAG AGAAATGAGG ATGAGGCAGT GTCGACTAAG
    CCCATGGTGA TTGGTTCTAT CAGACTTGTG GTCCAGGATT ACAGAGTTAG TCAGATTGAC
    TTATTTACTG AACCAGAAGG GTTAGGACTT CTAAATTCCT ACTTTGATGA TACTGAAGAA
    ATGCAAGGGT TTGGCATAAT ACAGAAGACT TGTTCCATTA AAGTACATTG GGGCACATGG
    CTGATTTATG AAGAGCCTGG ATTTCAGGGT GTCCCTTTTA TCCTGGAACC TGGTGAATAC
    CCTGACTTAT CCTTCTGGGA TACAGAGGAG GCATACATTG GATCCATGCG GCCACTGAAA
    ATGGGTGGCC GTAAAGTTGA ATTCCCTACA GATCCTAAGG TAGTTATTTA TGAAAAGCCT
    TTCTTTGAAG GAAAATGTAT GGAGCTGGAA ACAGAGATGG GTAGGTTTAT CATGGAAGGA
    GGTGAAACAG AAGAAACCAC TGGAGATGAA CATTTGTCAT TTGTATCAGT GGGATCTATG
    AAAGTTCTAC GAGGCATTTG GGTTGCTTAT GAGAAGCCTG ACTTTACAGG TCATCAGTAT
    TTGCTTGAAG AAGGAGAATA CAAGGACTGG AATGACTGGG GAGGCTACAG TGGAGAACTG
    CAGTCTTTAC GACCTATATT AGGTGATTTT TCAAATGCTC ACATGATAAT GTACAGTGAA
    AAAAACTTTG GATCCAAAGG TTCCAGTATT GATGTATTAG GAATTGTTGC TAATTTAAAG
    GAGACTGGAT ACGGAGTGAA GACACAGTCT ATTAATGTAC TGAGTGGAGT ATGGGTAGCC
    TATGAAAATC CTGACTTCAC AGGAGAACAG TACATACTGG ATAAAGGTTT CTATACCAGC
    TTTGAGGACT GGGGAGGCAA AAACTGTAAG ATCTCCTCTG TTCAACCCAT ATGTTTGGAT
    TCTTTCACTG GCCCAAGGAG ACGAAATCAG ATTCACTTGT TTTCGGAACC ACACTTTCAA
    GGTTGCAGTC AATGTTTTGG AGAAACAACA AGTCAAATTG ATGATTCCTT CTCTACCAAG
    TCTTGTAGAG TTTTAGGAGG CAGCTGGGTT GCATATGATG GAGAAAATTT CTCTGGTAAT
    CAGTACGTGT TGGAGGAAGG CCACTATCCT TCGCTGTCTG CAATGGGATG CCACCCTGGA
    GCAACTTTCA AGTCTCTTCG TTTTATAGAT GTTGAATTTT CTGAGCCAAC AATTATTCTT
    TTTGAAAGAG AAGACTTCAA AGGAAAAAAG ATTGAATTTA ATACAGAAAT AGTTAATCTC
    CGATCCCTAG GATTCAACAC ACACATACGC TCTGTCCAGG TGATTGGTGG CATATGGGTT
    ACTTACGAAT ATGGCAATTA CAGGGGCCGA CAGTTCCTAC TGTCACCAGC GGAAGTGCCT
    AAGTGGTATG AATTCAGTGG CTGTCACCAA ATAGGTTCTC TACGACCTTT TGTTCAGAAA
    CGAATTTATT TTAGACTTCG AAACAAAGCA ACAGGGTTAT TCATGTCAAC CAATGGAAAC
    CTAGAGGATC TGAAGCTTCT CAGAATACAG GTCATGGAGG ATGTCGGAGC TGATGACCAG
    ATCTGGATTT ATCAAGAAGG ATGCATCAAA TGCAGGATAG CAGAGGACTG CTGCCTGACC
    ATCTCGGGGA GCCTCGTAAC CTCTGGCTCA AAGCTGGGCC TGGCGCTCGA ACAGAATGCT
    GACAGTCAGT TCTGGAGCAT GAAGCCTGAC GGCAGGATCT ACAGCAAGCT TAAGCCAAAT
    TTAGTTTTAG ATATCAAAGG GGGTACACAG TATGATCAAA ATCACCTTAT CCTTAACACT
    GTCAGCAAAG AGAAGTTAAC ACAAGTGTGG GAAGCCATGG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51146
    Clone ID Related Accession (Same CDS sequence) XM_010808463.2 , XM_003582606.4 , XM_010808463.3
    Accession Version XM_010808463.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta/gamma crystallin domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808463.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCCAGAG CACAACCACC AGAAGAGAAC AAAAGGAAGC CAGTTTTGGG AAAACTTGGC 
    ACTCTGTTCA CGGCCGGAAG GAGAAGGAAC AGCAGAAATG GGTTAGAGAG TCCCACCAGC
    TCAAATACCA AACCAGGCTC TCCCAAAGAA GTAACCTCTT CCAGACTCCC GGAAAGAGAG
    ACTGAGAAGA GTCAACCTCT GAGCGGCCAA CCAACGCCAA CAGACGCATG CGAGGAGGGT
    TCCCCACAGG AGAAGTGCCA GGAGCCGGAG GGTGAACACC CCGAGGGCTG CGTGCAGGCA
    GCCCCCCCGG ACGCCAAGCG ATCACCTGGC CGGGGCAGCC ATGCAGCAGC GGCTGTGCAG
    CAGGGCGGTG AAAGTGATTC ACCCCAATTA GAACCTCTGG AGGCAGAGGG AGAGACCTTC
    CCAGATGCCA CCGCCACTGC CGAGCAGCTG CATTCCTCAT TAGAGAACTC CTCCAAGCGG
    GAGAACGCAG AGGCGCTCAT CCGCAGTCCC GGGGAGGACG CTTCGCCCGG TGCTGGCCGC
    GAGCAGGAGC CCACCCAGGG ATCGAGGGGC GTGCCGGGCT CGCCCGCCGG GGAGCATCCC
    GCCGACGGGG CCCCTCGCTT GTGTGCCCAC CGAGGCTCTC CGGAGCCCCA GGGTGGGCTG
    AGCCAAGCCC CGGAGGACTC TTCAGCTCCG CCGGGCGACC CGCTCGCCGA GGGTGCGGAG
    AACCGGGCGG GCTCAGCACC AGTCAACGTC AAAGCCGAGC CCCCGGGGCA AGACTGCCGA
    CCCCGCGAGC GCGCCCACCC AGCCAAAGTG CTGACTCTGG ACATCTACTT AAGTAAGACC
    GAGGCGGCAC AGGTGGACGA GCCGGTCGTG ATCAGCCCGG GGGCGGGAGA TTGCGGCGAT
    TGCGACGACA TGGAAAAGAG GTCGAGCGGC CGCAGGTCTG GGAGAAGGAG GCGGTCTCAG
    AGATCCACCG ACTCCCCCGG CGCTGACCCG CCGTTGCCCG ACTGTGAGCC CCGGGACGAC
    ACGGTGTTCG AGGACGAGGT GGCGCCAGAC GCGGCCGCCG AGAATGGCTG TGCAGAAAAG
    AAAGTGAAAT CTCCTCCGCA GGCGGCCCCC AATGGGGGCG TTGCCTCCGC TGCCGGCCTG
    GAGTCCAAGT CCAGCCCCAG CCCCAAAGGG CAGCTCCGAG GGGAGCCGGA GCGGAGCAAA
    CAGCCGCCGC CCGCCTCCTC CCCCACCAAG AGGAGGGGCC GGAGCCGCGT CCCCGAGGCT
    GTGCCCACCT CGCCCGCAGG CGGCCCGCGG CCTACGGCCA AGGACTCCCC TCTCAAGAGG
    GCGCCAGACC CCGTCCCCAG CCCGGCCGCC AAGGAAAGCG GGGAGGAGGC GGCCCGAGTC
    ATCCCCCGGG AGCTCACGGT TAAGAGCAGC TCGCTACTGC CTGAGATCAA GCCCGAGCAC
    AAGAGGGGGC CGCTCCCCCA CCTCTTGGAT GGCCGGGGAG ACGGAGGTCG AAGCAGAGAC
    TTGGGCAGAT CGGCTGGAGG TTCTGACGCC TTTGAGGGCT TGAAGCCCAG GAACCACTTC
    GGTGTGGGCA GATCGACGGT GACCACGAAA GTGACCCTCC CTGCCAGGCC CAAACACGTG
    GAACTAAATC TTAAAAACTC TAAGAATCTT GACAGTTTGG GAAGTGAGCA TAATCCATTT
    AGCCAGCCAG TTCACAAAGG AAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAGCGG
    GCAAACAGCA GCCCAAGACA CACTGACATT CGAGGTTTGA AGAACACTCC TGCGTCTAAT
    AAAACATTTG TCGAGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAGGA AATGGAGCTG
    CCTGAAAAGA AGGTGATGCC AAATAGTCAC CAGAACGGTG TGCCGGGGAA GCTGTCATCT
    GCAGAAGCCA AAGTGATGCT TCCTGAAGAG GAGACTCCTG TTTCTCAGCT TTCCCAAGGT
    GATAAGGTAG TTGTGCAAAC AGATGCTGGC TGCCCTTCCG AACCAGTGGT TGCTGCTCTG
    ACTCCCGTTA AGGACCAGGA GATCTTAGGA GAGAATGACT CCAAGGCTGC TAACAGCAAA
    AGACTTGAAC TTGAAAATAT GACTAATATG GCAGAAAACA CCCCTGCCAT TCTTGATACC
    AAAGATCCGC CTCCAACAGC CATACCAAAC CCGCAGCCTG GATTTTCTGA CTCACAGCCC
    CCAGCTGAGT CATCTAATGG GCCTTCTCTG TCACTGGCTG CCCCCATTCC TGCAGATGAT
    ACCAAAGACA CATGTGCTCA AGCTCCTGTA AGCAGTTCTC TGTGCACTGA TCTCAGAGAG
    TCAGAAAGCC ATAATGGATG TACTCTGCCT GTATCTCATC ATAATAATGA GACAATGCCC
    CCCTCCAAAC TTGCAGGAGG AAGAGAAGGA GGAGGGGAGA CAAGCCCTCC TTTGTCCCCA
    GAGCACAGCC CGCAAGCTGT GGGAGATGAG TGTCCATCCA AGGTCCTAGT CCAGGTCAGG
    TCCTTCATGC TCCCTGTGGA GAGCATGGAG GGGGTCTGCT CCCAGATCAT CACAGAAAGC
    TCTGAAGTTA GAGAAGCACA GTTGCCAAGC TGTCACAATA ATGAACTCCA AGTGGTTTCC
    GTTGCAAGTG GTGCTCCCCA GAAAGAGGAA GTACAGGGCA ATAAGGGCAC ATCACCCAAA
    CACATTCACT GCAAAGAGGA ACATATAGTA AAATCTGGCG CACAAGTCAT GCCGCCTGAA
    TCAGAAGCAA CTCTGCCTGT CCAGGCTCAA AGTGAGGACA ACGAAAAACC CCTGAAGGCT
    GAATCCACCG CCACCAGCCT CCCTGACAAC AGAAGTCATT TAGAAACCCC CCAAAGACCC
    AGTCAGAGTG CTGTGAATGG CCAGGGCAGT CCTGCCAGTC TTTTGAACAT CTCTGCTGGT
    AGTGATGATA GTGTATTCGA TTCTTCTTCT GACATGGAAA AATTCACTGA GATTATAAAA
    AAGATGGACA GCACTGTGTG TATGCCCCAG AAGAAAAAGA AGACCAGGCT ACCAAACTCC
    CCTGCCCCTC ACTTTGCCAT GCCACCTATT CACGAGGACA ATTTAGAAAA GGTGTTTGAT
    CCCAACGTGT TTACCTTTGG TTTGGGGAAG AAAAAGGAAG GCCAGTTGGA AATGTCACCA
    GCTTTACATT TGATGCAGAA TCTTGACACC AAATCCAAAC TGAGGCCCAA ACGATCATCC
    ACTGAACAGA GTGTCCTCTT CAAGTCCTTG CAGACTCATA CTAATGGGAA AGATGAGCCC
    CTGGCAGCTC CAGAAGTCAA TGACAAAGAG AACAAGGATG TCCAAAATGG TGGAGTCAAG
    AGATCGAGGC TAGAAAAAAG TGCACTTTTC TCAAGCTTGT TATCTTCTCT ACCACAAGAC
    AAAATCTTTT CTCCCTCTGT AACATCAGTC AATACCATGA CCACATCTTT CGGTACTGCT
    CATAGCAGTT CTCTTTCTCG ACCTTCCGTG TTGCAGCCTG TGGCTGAGAG TGCCCCACCC
    TGTAGTACAG AGAAAGAACA ACCAAGTCTG CCAGCCAGTA ACTTAAAGGT CTTCAATTTC
    AACTCGTCAG ATACGTCTCA TTCGGCATTG AAAAGTCTGA GCCCCATGGA AAAGTGCCCG
    CAAAAAGAGG AAACCAAAAA GGATCTGGAT CTGCAAAGCA ACCTTCCCAT GTCAGAAACT
    AAATTTTCTG AATTTTCAAA ACTGAAGACC AACGGTGATA CGTCTAATCA TACTGAAAGT
    GTTCTTAAAT CAAACTTGCC GAGCTATGGA AGCAGTGACA CAGACTTCAC TGGTCTCTTC
    AAAGCCAGCC GGTTTGACCC AAGCATTTCT TTTTCTGGAA TGGCATTATC AGATACAACA
    ACAATTAGAG GAAGCGTCCA GAATAAAATC AATCCCCGAC CTGGAAAAGT GGTGATATAC
    AGCGAGCCCG ACATCTCAGA GGCATGTATT GAAGTGTTCA GTGACGTGGA GGATTGTAGC
    ACCTGGAGCC TCTCTCCTGT GATACTAGTA AAAGTCGTTA GAGGATGTTG GATTTTGTAT
    GAGAAACCAA ATTTTGAAGG GCACTCCATC CCCTTAGAAG AAGGAGAATT GGAACTCTCT
    GGTCTCTGGG GTATAGAAGA TATTTTGGAG AGAAATGAGG ATGAGGCAGT GTCGACTAAG
    CCCATGGTGA TTGGTTCTAT CAGACTTGTG GTCCAGGATT ACAGAGTTAG TCAGATTGAC
    TTATTTACTG AACCAGAAGG GTTAGGACTT CTAAATTCCT ACTTTGATGA TACTGAAGAA
    ATGCAAGGGT TTGGCATAAT ACAGAAGACT TGTTCCATTA AAGTACATTG GGGCACATGG
    CTGATTTATG AAGAGCCTGG ATTTCAGGGT GTCCCTTTTA TCCTGGAACC TGGTGAATAC
    CCTGACTTAT CCTTCTGGGA TACAGAGGAG GCATACATTG GATCCATGCG GCCACTGAAA
    ATGGGTGGCC GTAAAGTTGA ATTCCCTACA GATCCTAAGG TAGTTATTTA TGAAAAGCCT
    TTCTTTGAAG GAAAATGTAT GGAGCTGGAA ACAGAGATGG GTAGGTTTAT CATGGAAGGA
    GGTGAAACAG AAGAAACCAC TGGAGATGAA CATTTGTCAT TTGTATCAGT GGGATCTATG
    AAAGTTCTAC GAGGCATTTG GGTTGCTTAT GAGAAGCCTG ACTTTACAGG TCATCAGTAT
    TTGCTTGAAG AAGGAGAATA CAAGGACTGG AATGACTGGG GAGGCTACAG TGGAGAACTG
    CAGTCTTTAC GACCTATATT AGGTGATTTT TCAAATGCTC ACATGATAAT GTACAGTGAA
    AAAAACTTTG GATCCAAAGG TTCCAGTATT GATGTATTAG GAATTGTTGC TAATTTAAAG
    GAGACTGGAT ACGGAGTGAA GACACAGTCT ATTAATGTAC TGAGTGGAGT ATGGGTAGCC
    TATGAAAATC CTGACTTCAC AGGAGAACAG TACATACTGG ATAAAGGTTT CTATACCAGC
    TTTGAGGACT GGGGAGGCAA AAACTGTAAG ATCTCCTCTG TTCAACCCAT ATGTTTGGAT
    TCTTTCACTG GCCCAAGGAG ACGAAATCAG ATTCACTTGT TTTCGGAACC ACACTTTCAA
    GGTTGCAGTC AATGTTTTGG AGAAACAACA AGTCAAATTG ATGATTCCTT CTCTACCAAG
    TCTTGTAGAG TTTTAGGAGG CAGCTGGGTT GCATATGATG GAGAAAATTT CTCTGGTAAT
    CAGTACGTGT TGGAGGAAGG CCACTATCCT TCGCTGTCTG CAATGGGATG CCACCCTGGA
    GCAACTTTCA AGTCTCTTCG TTTTATAGAT GTTGAATTTT CTGAGCCAAC AATTATTCTT
    TTTGAAAGAG AAGACTTCAA AGGAAAAAAG ATTGAATTTA ATACAGAAAT AGTTAATCTC
    CGATCCCTAG GATTCAACAC ACACATACGC TCTGTCCAGG TGATTGGTGG CATATGGGTT
    ACTTACGAAT ATGGCAATTA CAGGGGCCGA CAGTTCCTAC TGTCACCAGC GGAAGTGCCT
    AAGTGGTATG AATTCAGTGG CTGTCACCAA ATAGGTTCTC TACGACCTTT TGTTCAGAAA
    CGAATTTATT TTAGACTTCG AAACAAAGCA ACAGGGTTAT TCATGTCAAC CAATGGAAAC
    CTAGAGGATC TGAAGCTTCT CAGAATACAG GTCATGGAGG ATGTCGGAGC TGATGACCAG
    ATCTGGATTT ATCAAGAAGG ATGCATCAAA TGCAGGATAG CAGAGGACTG CTGCCTGACC
    ATCTCGGGGA GCCTCGTAAC CTCTGGCTCA AAGCTGGGCC TGGCGCTCGA ACAGAATGCT
    GACAGTCAGT TCTGGAGCAT GAAGCCTGAC GGCAGGATCT ACAGCAAGCT TAAGCCAAAT
    TTAGTTTTAG ATATCAAAGG GGGTACACAG TATGATCAAA ATCACCTTAT CCTTAACACT
    GTCAGCAAAG AGAAGTTAAC ACAAGTGTGG GAAGCCATGG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806765.1
    CDS140..6187
    Translation

    Target ORF information:

    RefSeq Version XM_010808463.3
    Organism Bos taurus(cattle)
    Definition Bos taurus crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808463.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGTCCAGAG CACAACCACC AGAAGAGAAC AAAAGGAAGC CAGTTTTGGG AAAACTTGGC 
    ACTCTGTTCA CGGCCGGAAG GAGAAGGAAC AGCAGAAATG GGTTAGAGAG TCCCACCAGC
    TCAAATACCA AACCAGGCTC TCCCAAAGAA GTAACCTCTT CCAGACTCCC GGAAAGAGAG
    ACTGAGAAGA GTCAACCTCT GAGCGGCCAA CCAACGCCAA CAGACGCATG CGAGGAGGGT
    TCCCCACAGG AGAAGTGCCA GGAGCCGGAG GGTGAACACC CCGAGGGCTG CGTGCAGGCA
    GCCCCCCCGG ACGCCAAGCG ATCACCTGGC CGGGGCAGCC ATGCAGCAGC GGCTGTGCAG
    CAGGGCGGTG AAAGTGATTC ACCCCAATTA GAACCTCTGG AGGCAGAGGG AGAGACCTTC
    CCAGATGCCA CCGCCACTGC CGAGCAGCTG CATTCCTCAT TAGAGAACTC CTCCAAGCGG
    GAGAACGCAG AGGCGCTCAT CCGCAGTCCC GGGGAGGACG CTTCGCCCGG TGCTGGCCGC
    GAGCAGGAGC CCACCCAGGG ATCGAGGGGC GTGCCGGGCT CGCCCGCCGG GGAGCATCCC
    GCCGACGGGG CCCCTCGCTT GTGTGCCCAC CGAGGCTCTC CGGAGCCCCA GGGTGGGCTG
    AGCCAAGCCC CGGAGGACTC TTCAGCTCCG CCGGGCGACC CGCTCGCCGA GGGTGCGGAG
    AACCGGGCGG GCTCAGCACC AGTCAACGTC AAAGCCGAGC CCCCGGGGCA AGACTGCCGA
    CCCCGCGAGC GCGCCCACCC AGCCAAAGTG CTGACTCTGG ACATCTACTT AAGTAAGACC
    GAGGCGGCAC AGGTGGACGA GCCGGTCGTG ATCAGCCCGG GGGCGGGAGA TTGCGGCGAT
    TGCGACGACA TGGAAAAGAG GTCGAGCGGC CGCAGGTCTG GGAGAAGGAG GCGGTCTCAG
    AGATCCACCG ACTCCCCCGG CGCTGACCCG CCGTTGCCCG ACTGTGAGCC CCGGGACGAC
    ACGGTGTTCG AGGACGAGGT GGCGCCAGAC GCGGCCGCCG AGAATGGCTG TGCAGAAAAG
    AAAGTGAAAT CTCCTCCGCA GGCGGCCCCC AATGGGGGCG TTGCCTCCGC TGCCGGCCTG
    GAGTCCAAGT CCAGCCCCAG CCCCAAAGGG CAGCTCCGAG GGGAGCCGGA GCGGAGCAAA
    CAGCCGCCGC CCGCCTCCTC CCCCACCAAG AGGAGGGGCC GGAGCCGCGT CCCCGAGGCT
    GTGCCCACCT CGCCCGCAGG CGGCCCGCGG CCTACGGCCA AGGACTCCCC TCTCAAGAGG
    GCGCCAGACC CCGTCCCCAG CCCGGCCGCC AAGGAAAGCG GGGAGGAGGC GGCCCGAGTC
    ATCCCCCGGG AGCTCACGGT TAAGAGCAGC TCGCTACTGC CTGAGATCAA GCCCGAGCAC
    AAGAGGGGGC CGCTCCCCCA CCTCTTGGAT GGCCGGGGAG ACGGAGGTCG AAGCAGAGAC
    TTGGGCAGAT CGGCTGGAGG TTCTGACGCC TTTGAGGGCT TGAAGCCCAG GAACCACTTC
    GGTGTGGGCA GATCGACGGT GACCACGAAA GTGACCCTCC CTGCCAGGCC CAAACACGTG
    GAACTAAATC TTAAAAACTC TAAGAATCTT GACAGTTTGG GAAGTGAGCA TAATCCATTT
    AGCCAGCCAG TTCACAAAGG AAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAGCGG
    GCAAACAGCA GCCCAAGACA CACTGACATT CGAGGTTTGA AGAACACTCC TGCGTCTAAT
    AAAACATTTG TCGAGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAGGA AATGGAGCTG
    CCTGAAAAGA AGGTGATGCC AAATAGTCAC CAGAACGGTG TGCCGGGGAA GCTGTCATCT
    GCAGAAGCCA AAGTGATGCT TCCTGAAGAG GAGACTCCTG TTTCTCAGCT TTCCCAAGGT
    GATAAGGTAG TTGTGCAAAC AGATGCTGGC TGCCCTTCCG AACCAGTGGT TGCTGCTCTG
    ACTCCCGTTA AGGACCAGGA GATCTTAGGA GAGAATGACT CCAAGGCTGC TAACAGCAAA
    AGACTTGAAC TTGAAAATAT GACTAATATG GCAGAAAACA CCCCTGCCAT TCTTGATACC
    AAAGATCCGC CTCCAACAGC CATACCAAAC CCGCAGCCTG GATTTTCTGA CTCACAGCCC
    CCAGCTGAGT CATCTAATGG GCCTTCTCTG TCACTGGCTG CCCCCATTCC TGCAGATGAT
    ACCAAAGACA CATGTGCTCA AGCTCCTGTA AGCAGTTCTC TGTGCACTGA TCTCAGAGAG
    TCAGAAAGCC ATAATGGATG TACTCTGCCT GTATCTCATC ATAATAATGA GACAATGCCC
    CCCTCCAAAC TTGCAGGAGG AAGAGAAGGA GGAGGGGAGA CAAGCCCTCC TTTGTCCCCA
    GAGCACAGCC CGCAAGCTGT GGGAGATGAG TGTCCATCCA AGGTCCTAGT CCAGGTCAGG
    TCCTTCATGC TCCCTGTGGA GAGCATGGAG GGGGTCTGCT CCCAGATCAT CACAGAAAGC
    TCTGAAGTTA GAGAAGCACA GTTGCCAAGC TGTCACAATA ATGAACTCCA AGTGGTTTCC
    GTTGCAAGTG GTGCTCCCCA GAAAGAGGAA GTACAGGGCA ATAAGGGCAC ATCACCCAAA
    CACATTCACT GCAAAGAGGA ACATATAGTA AAATCTGGCG CACAAGTCAT GCCGCCTGAA
    TCAGAAGCAA CTCTGCCTGT CCAGGCTCAA AGTGAGGACA ACGAAAAACC CCTGAAGGCT
    GAATCCACCG CCACCAGCCT CCCTGACAAC AGAAGTCATT TAGAAACCCC CCAAAGACCC
    AGTCAGAGTG CTGTGAATGG CCAGGGCAGT CCTGCCAGTC TTTTGAACAT CTCTGCTGGT
    AGTGATGATA GTGTATTCGA TTCTTCTTCT GACATGGAAA AATTCACTGA GATTATAAAA
    AAGATGGACA GCACTGTGTG TATGCCCCAG AAGAAAAAGA AGACCAGGCT ACCAAACTCC
    CCTGCCCCTC ACTTTGCCAT GCCACCTATT CACGAGGACA ATTTAGAAAA GGTGTTTGAT
    CCCAACGTGT TTACCTTTGG TTTGGGGAAG AAAAAGGAAG GCCAGTTGGA AATGTCACCA
    GCTTTACATT TGATGCAGAA TCTTGACACC AAATCCAAAC TGAGGCCCAA ACGATCATCC
    ACTGAACAGA GTGTCCTCTT CAAGTCCTTG CAGACTCATA CTAATGGGAA AGATGAGCCC
    CTGGCAGCTC CAGAAGTCAA TGACAAAGAG AACAAGGATG TCCAAAATGG TGGAGTCAAG
    AGATCGAGGC TAGAAAAAAG TGCACTTTTC TCAAGCTTGT TATCTTCTCT ACCACAAGAC
    AAAATCTTTT CTCCCTCTGT AACATCAGTC AATACCATGA CCACATCTTT CGGTACTGCT
    CATAGCAGTT CTCTTTCTCG ACCTTCCGTG TTGCAGCCTG TGGCTGAGAG TGCCCCACCC
    TGTAGTACAG AGAAAGAACA ACCAAGTCTG CCAGCCAGTA ACTTAAAGGT CTTCAATTTC
    AACTCGTCAG ATACGTCTCA TTCGGCATTG AAAAGTCTGA GCCCCATGGA AAAGTGCCCG
    CAAAAAGAGG AAACCAAAAA GGATCTGGAT CTGCAAAGCA ACCTTCCCAT GTCAGAAACT
    AAATTTTCTG AATTTTCAAA ACTGAAGACC AACGGTGATA CGTCTAATCA TACTGAAAGT
    GTTCTTAAAT CAAACTTGCC GAGCTATGGA AGCAGTGACA CAGACTTCAC TGGTCTCTTC
    AAAGCCAGCC GGTTTGACCC AAGCATTTCT TTTTCTGGAA TGGCATTATC AGATACAACA
    ACAATTAGAG GAAGCGTCCA GAATAAAATC AATCCCCGAC CTGGAAAAGT GGTGATATAC
    AGCGAGCCCG ACATCTCAGA GGCATGTATT GAAGTGTTCA GTGACGTGGA GGATTGTAGC
    ACCTGGAGCC TCTCTCCTGT GATACTAGTA AAAGTCGTTA GAGGATGTTG GATTTTGTAT
    GAGAAACCAA ATTTTGAAGG GCACTCCATC CCCTTAGAAG AAGGAGAATT GGAACTCTCT
    GGTCTCTGGG GTATAGAAGA TATTTTGGAG AGAAATGAGG ATGAGGCAGT GTCGACTAAG
    CCCATGGTGA TTGGTTCTAT CAGACTTGTG GTCCAGGATT ACAGAGTTAG TCAGATTGAC
    TTATTTACTG AACCAGAAGG GTTAGGACTT CTAAATTCCT ACTTTGATGA TACTGAAGAA
    ATGCAAGGGT TTGGCATAAT ACAGAAGACT TGTTCCATTA AAGTACATTG GGGCACATGG
    CTGATTTATG AAGAGCCTGG ATTTCAGGGT GTCCCTTTTA TCCTGGAACC TGGTGAATAC
    CCTGACTTAT CCTTCTGGGA TACAGAGGAG GCATACATTG GATCCATGCG GCCACTGAAA
    ATGGGTGGCC GTAAAGTTGA ATTCCCTACA GATCCTAAGG TAGTTATTTA TGAAAAGCCT
    TTCTTTGAAG GAAAATGTAT GGAGCTGGAA ACAGAGATGG GTAGGTTTAT CATGGAAGGA
    GGTGAAACAG AAGAAACCAC TGGAGATGAA CATTTGTCAT TTGTATCAGT GGGATCTATG
    AAAGTTCTAC GAGGCATTTG GGTTGCTTAT GAGAAGCCTG ACTTTACAGG TCATCAGTAT
    TTGCTTGAAG AAGGAGAATA CAAGGACTGG AATGACTGGG GAGGCTACAG TGGAGAACTG
    CAGTCTTTAC GACCTATATT AGGTGATTTT TCAAATGCTC ACATGATAAT GTACAGTGAA
    AAAAACTTTG GATCCAAAGG TTCCAGTATT GATGTATTAG GAATTGTTGC TAATTTAAAG
    GAGACTGGAT ACGGAGTGAA GACACAGTCT ATTAATGTAC TGAGTGGAGT ATGGGTAGCC
    TATGAAAATC CTGACTTCAC AGGAGAACAG TACATACTGG ATAAAGGTTT CTATACCAGC
    TTTGAGGACT GGGGAGGCAA AAACTGTAAG ATCTCCTCTG TTCAACCCAT ATGTTTGGAT
    TCTTTCACTG GCCCAAGGAG ACGAAATCAG ATTCACTTGT TTTCGGAACC ACACTTTCAA
    GGTTGCAGTC AATGTTTTGG AGAAACAACA AGTCAAATTG ATGATTCCTT CTCTACCAAG
    TCTTGTAGAG TTTTAGGAGG CAGCTGGGTT GCATATGATG GAGAAAATTT CTCTGGTAAT
    CAGTACGTGT TGGAGGAAGG CCACTATCCT TCGCTGTCTG CAATGGGATG CCACCCTGGA
    GCAACTTTCA AGTCTCTTCG TTTTATAGAT GTTGAATTTT CTGAGCCAAC AATTATTCTT
    TTTGAAAGAG AAGACTTCAA AGGAAAAAAG ATTGAATTTA ATACAGAAAT AGTTAATCTC
    CGATCCCTAG GATTCAACAC ACACATACGC TCTGTCCAGG TGATTGGTGG CATATGGGTT
    ACTTACGAAT ATGGCAATTA CAGGGGCCGA CAGTTCCTAC TGTCACCAGC GGAAGTGCCT
    AAGTGGTATG AATTCAGTGG CTGTCACCAA ATAGGTTCTC TACGACCTTT TGTTCAGAAA
    CGAATTTATT TTAGACTTCG AAACAAAGCA ACAGGGTTAT TCATGTCAAC CAATGGAAAC
    CTAGAGGATC TGAAGCTTCT CAGAATACAG GTCATGGAGG ATGTCGGAGC TGATGACCAG
    ATCTGGATTT ATCAAGAAGG ATGCATCAAA TGCAGGATAG CAGAGGACTG CTGCCTGACC
    ATCTCGGGGA GCCTCGTAAC CTCTGGCTCA AAGCTGGGCC TGGCGCTCGA ACAGAATGCT
    GACAGTCAGT TCTGGAGCAT GAAGCCTGAC GGCAGGATCT ACAGCAAGCT TAAGCCAAAT
    TTAGTTTTAG ATATCAAAGG GGGTACACAG TATGATCAAA ATCACCTTAT CCTTAACACT
    GTCAGCAAAG AGAAGTTAAC ACAAGTGTGG GAAGCCATGG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.