SMG1 cDNA ORF clone, Bos taurus(cattle)

The following SMG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa37723 NM_001205915.1
Latest version!
Bos taurus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa230306 XM_024984919.1
Latest version!
Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa32978 XM_005224744.4
Latest version!
Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa32979 XM_005224745.4
Latest version!
Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OBa32978 XM_005224744.3 Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa32979 XM_005224745.3 Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa37723
    Clone ID Related Accession (Same CDS sequence) NM_001205915.1
    Accession Version NM_001205915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-29
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase SMG1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Apr 20, 2011 this sequence version replaced XM_603490.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
    CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA
    AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT
    TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT
    GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG
    TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG
    CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT
    TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA
    GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG
    CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA
    AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT
    GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA
    TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT
    GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC
    ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG
    GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA
    GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG
    TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT
    CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG
    TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC
    CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT
    CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT
    GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT
    TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA
    TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT
    TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA
    CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGGTTGTT
    GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG CTCAGTCTGA AGAATATTCC TGTTTTAGAG
    ACTGCCTATA AATTAATTTT GGGAGAAATG ACTTGTGCCC TAAACAATCT GCTACATAGT
    CTACAACTTC CTGATGCCTG TTCTGAAATA AAACATGAGG CTTTTAAGAA TCATGTTTTC
    AATGTAGACA ATGCAAAATT TGTAGTTATA TTTGACCTTA GTGCTCTGAC TACAATTGGA
    AATGCTAAAA ACTCACTAAT TGGGATGTGG GCCCTGTCTC CAACTGTGTT TGCACTTCTG
    AGTAAGAATC TGATGATTGT GCATGGCGAC CTGGCTGTTC ATTTCCCTGC CATTCAGTAT
    GCAGTGCTCT ACACATTGTA TTCTCATTGT ACCAGGCATG ACCACTTTAT ATCTAGTAGT
    CTTAGTTCAT CATCCCCGTC CTTGTTTGAT GGTGCCGTTA TTAGTACTGT AACTACAGCT
    ACAAAAAAAC ATTTCTCAAT TATCTTAAAT CTCCTGGGAA TATTGCTTAA GAAAGATAAT
    CTTAACCAGG ACACAAGGAA ACTGTTAATG ACCTGGGCTT TGGAAGTGGC TGTTTTAATG
    AAGAAGTCTG AAACATATGC ACCTTTATTC TCTCTTCCAT CTTTCCATAA ATTTTGCAAA
    GGCCTTTTAG CCAACACTCT GGTCGAAGAT GTGAATATCT GTCTGCAAGC ATGCAGCAGT
    CTACATGCAC TGTCCTCTTC CTTACCAGAT GATCTTTTAC AGAGGTGTGT TGATGTTTGC
    CGTGTTCAGC TAGTCCATAG TGGAACTCGT ATTCGACAAG CATTTGGAAA GCTATTGAAG
    TCAATCCCTT TAGATGTTGT CTTGAGCAAT AACAATCACA CAGAAATTCA AGAAATTTCT
    TTAGCATTAA GAAGTCACAT GAGTAAAGCA CCAAGTAACA CATTCCACCC ACAAGATTTC
    TCTGATGTTA TTAGTTTTAT TTTGTATGGA AACTCTCATA GAATAGGGAA GGACAATTGG
    TTAGAAAGAT TGTTCTATAG CTGCCAGAGA CTGGATAAAC GTGACCAATC AACGATTCCC
    CGCAATCTGC TAAAGACAGA TGCTGTCCTC TGGCAGTGGG CTATTTGGGA AGCAGCACAG
    TTCACTGTCC TTTCTAAGCT GAGGACCCCA CTTGGCAGAG CTCAGGATAC CTTCCAAACA
    ATTGAAGGTA TCATTAGAAG TCTTGCAGCT CACACATTAA ACCCCGATCA AGATGTTAGT
    CAGTGGACAA CTGCAGACAA TGACGAAGGC CACAGTAGCA ACCAGCTTAG ACTTGTGCTG
    CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG TTAATGTATA ATGCATATGA AGGATGTGCC
    AATGCATTAA CATCGCCTCC CAAGGTCATT AGGACTTTTT TCTATACCAA TCGCCAAACT
    TGTCAAGACT GGCTGACTCG GATCCGACTC TCCATCATGA GGGTTGGATT GTTGGCAGGC
    CAGCCTGCTG TGACAGTGAG ACACGGCTTC GATTTGCTTA CAGAAATGAA GACAAATCCG
    TCTCAGGGAA ATGAATTGGA AGTAACTATC ATGATGGTGG TAGAAGCATT GTGTGAACTT
    CATTGTCCTG AAGCTATACA GGGAATTGCT GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT
    CTTCTCTGGA TTAATTCAGT GGCCCAACAG GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG
    GAGTACCAGG AGCACCTGTG TGCCATGACA GGTGTCGATT GCTGCATCTC TAGCTTCGAC
    AAATCCGTTC TCACATTGGC TAATACTGGG CGTAATAGTG CCAGCCCCAA GCATTCTTTG
    AATGGTGAAT CCAGAAAAAC TGTGCTTTCC AAACCAACTG ATTCTTCTCC TGAGGTTATA
    AATTATTTGG GAAATAAAGC ATGTGAGTGC TACATTTCAA TAGCTGATTG GGCTGCTGTA
    CAGGAGTGGC AGAATGCTGT CCATGACTTG AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC
    CTAAAGGCTG ATTTCAACTA CATAAAATCA CTAAGCAGTT TTGAATCTGG AGAATTTATT
    GAATGCACTG AACAATTAGA ATTGTTACCA GGAGAAAATA TCAGTCTACT TGCTGGAGGA
    TCAAAAGAAA AAATAGATAT GAAAAAACTG CTTCCTAACA TGTTAAGTCC AGATCCAAGA
    GAACTTCAGA AATCCATTGA AGTTCAGTTG TTAAGAAGTT CTGTTTGTTT GGCAACTGCT
    TTAAACCACA TAGAACAAGA TCAGAAGTGG CAGTCTATTA CCGAAAATGT GGTGAAGTAC
    CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA CCCCTGAGAC TTTCTACTCT AACAGTTTCC
    CAGTCTCTGC CAGTCCTAAG TACTTTACAG CTGTATTGCT CTTCTGCTTT GGAGAACACA
    GTTTCCAACA GACTTGCCAC AGAGGACTGT CTTATTCCAC TCTTTAGTGA AGCTTTGCGT
    TCATGTAAAC AGCATGATGT CAGGCCATGG ATGCAAGCAT TAAGATACAC GATGTACCAG
    AGTCAGTTGT TGGAGAAAAT AAAAGAACAA ACAGTTCCAA TTAGAAGCCA TCTCATGGAA
    TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA AAACGGGGGA ATGTGTCACT TGCAACAAGA
    TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG GGAAAGACTA CCACTGCACA GGATTTAGTC
    CAGCACTTTA AAAAACTATC AACTCAAGGT CAAGTGGATG AAAAATGGGG GCCTGAACTT
    GACATTGAAA AAACGAAACT GCTATATACA GCAGGCCAGT CAACACATGC AATGGAGATG
    TTGAGTTCCT GTGCCATCTC TTTCTGCAAG TCTGCCAAAG CTGAGCATGC TGTTGCCAAG
    TCAATTTTGA CATTGGCTAA ATGGATCCAG GCAGAATGGA AAGAAATCTC AGGACAGCTG
    AAACAGGTTT ACAGAGCTCA GCAGCAGCAG AACTTCACGG GTCTTTCTAC TTTGTCTAAA
    AACATACTCA CTCTAATTGA ACTACCATCT GTTAATACGA TGGAGGAAGA GTATCCTCGG
    ATAGAGAGTG AATCTACAGT ACATATTGGA GTTGGGGAAC CTGACTTCAT CTTGGGACAG
    CTGTATCATC TGTCTTCAGT ACAGGCACCT GAAGTAGCCA AATCTTGGGC CGCATTGGCT
    AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG GTTGACAATG CCAGTCAGGG AGAAGGTGTT
    CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT CAGAATCTGC TCCCAGACAC CATAACCGAA
    GAAGAAAAAG AGAGAATATA TGGTATTCTT GGTCAGGCTG TGTGTCGCCC AGCGGGGATT
    CAGGATGAAG ATATAACGCT TCAGATCACA GAAAGTGAAG ATAATGAGGA AGATGACATG
    GTTGATGTTA TCTGGCGTCA GTTAATATCA AGTTGCCCAT GGCTTTCAGA ACTTGATGAA
    AGTGCAACAG AAGGAGTCAT TAAAGTGTGG AGGAAAGTTG TGGACAGAAT CTTCAGCCTG
    TATAAACTGT CCTGCAGCGC ATATTTTACT TTCCTTAAAC TCAATGCTGG GCAGATTCCT
    TTAGATGAAG ATGACCCTCG GCTCCATTTA AGCCACAGAG TGGAGCAGAG CACGGATGAC
    ATGATTGTGA TGGCCACGCT GCGGTTGCTC AGGTTGCTCG TGAAGCACGC TGGGGAGCTT
    CGGCAGTATC TGGAACATGG CTTGGAAACA ACACCTACTG CGCCTTGGAG AGGAATTATT
    CCGCAGCTTT TCTCACGCTT AAACCACCCT GAAGTGTATG TGCGCCAGAG TATTTGTAAC
    CTCCTCTGCC GCGTGGCTCA GGATTCCCCG CATCTCATAC TGTATCCTGC AATAGTGGGC
    ACCATATCGC TTAGTAGTGA GTCACAGGCT TCAGGAAATA AATTTTCCTC TGCAATTCCA
    ACTTTACTTG GCAATATTCA AGGAGAAGAA CTACTGGTTT CTGAATGTGA GGGAGGAAGT
    CCTCCTGCTT CTCAAGATAG CAATAAGGAT GAACCTAAAA GTGGACTGAA TGAAGACCAA
    GCCATGATGC AGGACTGCTA CAGCAAAATT GTAGATAAAC TGTCCTCGGC AAACCCAACT
    ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT GAGCTGCGCA GGGTCACTGT TCTCTGGGAT
    GAGCTCTGGT TGGGAGTTCT GTTACAGCAG CACATGTATG TCCTGAGACG AATTCAGCAG
    TTGGAAGACG AGGTGAAGAG AGTCCAGAAC AACAACACCT TACGCAAAGA AGAGAAAATC
    GCAATCATGC GGGAGAAGCA CACAGCTTTG ATGAAGCCCA TCGTATTTGC TTTGGAGCAC
    GTTAGGAGCA TCACAGCGGC CCCTGCAGAA ACACCTCATG AAAAGTGGTT TCAGGATAAC
    TATGGTGATG CCATTGAAAA TGCTCTAGAA AAGCTGAAAA CACCGTCAAA CCCTGCAAAG
    CCTGGAAGCA GCTGGATTCC ATTTAAAGAG ATAATGCTGA GTTTGCAACA GAGAGCACAG
    AAACGTGCAA GTTACATCTT GCGTCTTGAA GAAATCAGCC CATGGCTGGC TGCCATGACT
    AACACTGAAA TTGCTCTTCC CGGAGAAGTC TCAGCCAGAG ATACCGTCAC AATCCACAGT
    GTTGGTGGAA CCATCACAAT CTTACCTACT AAAACCAAGC CAAAGAAACT TCTCTTTCTA
    GGATCAGATG GGAAGAGCTA TCCTTATCTT TTCAAAGGAT TGGAGGATTT ACATCTGGAT
    GAGAGAATAA TGCAATTTCT GTCTATTGTA AATACCATGT TTGCAACAAT CAATCGCCAG
    GAAACACCTC GTTTCCATGC TCGCCACTAT TCTGTAACAC CACTGGGAAC TCGCTCAGGC
    CTAATCCAGT GGGTGGATGG AGCCACGCCC TTATTTGGTC TTTACAAACG ATGGCAACAA
    CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC CAAGATTCCT ACCAAACTCC TCAGAATCCT
    GGAATCGTAC CCCGTCCTAG TGAACTTTAT TATAGTAAAA TTGGTCCTGC TTTGAAAGCA
    GTTGGGCTTA GTCTGGATGT GTCCCGCCGG GATTGGCCTC TTCATGTGAT GAAGGCAGTG
    TTAGAAGAGT TAATGGAGGC CACGCCCCCA AATCTCCTTG CTAAAGAGCT TTGGTCATCA
    TGTACCACCC CTGATGAGTG GTGGAGAGTC ACACAGTCTT ATGCCAGATC TACTGCAGTC
    ATGTCTATGG TCGGATACAT AATTGGCCTT GGAGATAGAC ATCTGGATAA TGTTCTCATA
    GATATGACGA CTGGAGAGGT TGTTCACATA GATTACAATG TTTGCTTTGA AAAAGGTAAA
    AGCCTTAGAG TTCCTGAGAA AGTACCTTTT CGAATGACAC AGAACATTGA AACAGCACTG
    GGTGTAACTG GAGTAGAAGG TGTTTTTAGG CTTTCATGTG AGCAGGTTCT ACACATTATG
    CGGCGTGGCA GAGAGACTCT GCTGACGCTG CTGGAGGCCT TTGTATATGA CCCCCTGGTG
    GACTGGACAG CTGGGGGCGA GGCTGGGTTT GCTGGTGCGG TCTATGGTGG AGGGGGCCAG
    CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG ATGGAACGAG AGATTACTCG CAGCCTTTTT
    TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC TGGTTTAAAA ACAGAGATGA GATGCTGGTT
    GTACTTCCCA AGCTGGACAG CAGCTTAGAT GAATACCTGA GCTTGCAAGA GCAACTAACA
    GATGTGGAGA AACTGCAGGG CAAACTGCTG GAAGAAATTG AGTTTCTAGA AGGAGCTGAA
    GGAGTGGATC ATCCTTCTCA TACTCTGCAA CACAGGTATT CTGAACACAC TCAACTACAG
    ACTCAGCAGA GAGCTGTTCA GGAAGCAATC CAGGTGAAGC TGAATGAATT TGAACAGTGG
    ATAACACACT ATCAGGCTGC GTTCAATAAT TTGGAAGCTA CACAGCTGGC CAGTTTGCTT
    CAGGAGATAA GCACACAGAT GGACCTTGGT CCTCCCAGCT ACGTGCCGGC AACAGCCTTT
    CTGCAGAATG CTGGCCAGGC CCACTTGATT AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT
    GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG CTGCGAGGCT GTCTGGAGCA GCTGCATCAC
    TACGCAACTG TTGCTCTGCA GTATCCAAAG GCCATATTTC AGAAACATCG AATTGAGCAG
    TGGAAGACCT GGATGGAAGA GCTCATCTGT AATACCACAG TAGAACGCTG TCAGGAACTT
    TACAGGAAAT ATGAAATGCA GTATGCGCCC CAGCCACCCC CAACAGTGTG TCAGTTCATC
    ACGGCCACTG AGATGACCCT GCAGCGGTAC GCAGCCGACA TCAACAGCCG ACTTATCAGA
    CAGGTGGAAC GCTTGAAACA GGAAGCTGTC ACTGTACCGG TTTGTGAAGA TCAGTTGAAA
    GAAATTGAAC GTTGTATCAA AGTTTTCCTT CACGAGAATG GAGAGGAAGG ATCTTTGAGT
    CTAGCAAGTG TTATTATTTC TGCCCTTTGT ACTCTCACAA GGCGTAACCT AATGATGGAA
    GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG GTTGATCTGA CTTCTCGGGA TGGAGCCTGG
    TTCTTGGAGG AACTCTGCAG TATGAGCGGA AATGTCACAT GTCTGGTTCA GTTACTGAAG
    CAGTGTCATT TAGTGCCACA AGACTTAGAC ATTCCTAACC CCATGGAAGC ATCTGAGGCC
    GTTCATTTGG CTAATGGTGT ATACACCTCA CTTCAGGAAT TAAATTCAAA TTTCCGGCAA
    ATCATATTTC CAGAAGCACT GAGATGTTTA ATGAAAGGAG AATACACGTT AGAAAATATG
    CTTCATGAGC TGGACAGTCT TATTGAGCAG ACAACTGATG GTGTTCCTCT GCAGACTCTA
    GTTGAATCTC TTCAAGCCTA TTTAAGAAAT GCAGCTATGG GACTTGAGGA AGAAACACAT
    GCTCATTACA TCGATGTTGC CAGAATGCTT CATGCTCAGT ATGGTGAATT AATCCAACCA
    AGAAATGGTT CAGTTGATGA AACACCAAAA ATGTCAGCTG GCCAGATGCT TTTGGTAGCA
    TTTGATGGCA TGTTTGCCCA AGTTGAGACT GCTTTTGGCT TATTGGTTGA AAAGCTAAAT
    AAGATGGAAA TTCCTATAGC TTGGCGAAAG ATTGACATAA TACGGGAAGC CAGGAGCACT
    CAGGTCAATT TTTTTGATGA TGATAATCAC CGACAGGTGC TGGAAGAGAT CTTCTTTCTA
    AAACGACTAC AGACAATTAA GGAGTTCTTT CGGCTCTGTG GTACCTTTTC TAAAACTTTG
    TCAGGATCAA GTTCACTTGA AGATCAGAAT ACTGTAAATG GGCCTGTACA GATTGTCAAT
    GTGAAAACCC TTTTTAGAAA CTCTTGTTTC AGTGAAGACC AGATGGCCAA ACCAATAAAG
    GCATTCACAG CTGACTTTGT GCGGCAGCTC CTGATTGGAC TCCCGAACCA AGCCCTGGGC
    CTCACACTGT GCAGCTTCAT CAGTGCTCTA GGGGTAGACA TCATTGCGCA GGTGGAGGCG
    AAGGATTTTG GTGCTGAAAG CAAAGTTTCT GTTGACGATC TGTGTAAGAA GGCGGTGGAG
    CACAACATCC AGATCGGGAA GTTCTCTCAG CTGGTCATGA ACAGGGCGAC TGTGCTGGCG
    AGCTCCTACG ACACTGCCTG GAAGAAGCAC GACTTGGTGC GCAGGCTGGA GACCAGCATC
    GCCTCCTGCA AGACAAGCCT GCAGCGTGTC CAGCTGCACA TCGCCATGTT TCAGTGGCAG
    CATGAAGATC TCCTTATCAA TAGACCACAA GCCATGTCTG TCACACCTCC CCCTCGTTCT
    GCCATATTAA CCAGCATGAA AAAGAAACTC CATACTCTGA GCCAGATTGA AACTTCAATT
    GCAACAGTTC AGGAGAAACT GGCAGCACTT GAAGCAAGTA TTGAACAGCG ACTCAAATGG
    GCAGGTGGTG CCAACCCTGC ACTGGCACCT GTACTACAGG ATTTTGAAGC AACAATAGCT
    GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC
    AGCAATATCA TTCATTTTGA AAGTTTACGA ACTAGAACTG CAGAAGCCTT AAACCTGGAT
    GCTGCATTAT TTGAACTAAT CAAACGGTGT CAGCAAATGT GTTCATTTGC ATCGCAGTTT
    AACAGTTCAG TCTCTGAGTT AGAGCTTCGT TTATTACAGA GGGTGGACAC GACTCTTGAA
    CATCCTATTG GCAGCTCTGA GTGGCTTTTG TCAGCACACA AACAACTGAC CCAGGATATG
    TCTACTCAGA GAGCAATTCA GACAGAGAAA GAGCAACAAA TAGAAACAGT CTGTGAAACA
    ATTCAGAATC TGGTTGATAA TATAAAGACT GTGCTCACTG GCCATAACCG GCAGCTTGGA
    GATGTCAAAC ATCTCCTGAA AGCAATGGCC AAGGATGAAG AAGCTGCTCT GGCAGATGGT
    GAAGATGTTC CCTATGAGAA CAGTGTTAGG CAGTTCTTGG GTGAATATAA ATCATGGCAA
    GACAACATTC AGACAGTGCT GTTTACATTA GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA
    GAACATGTTG AAATGCTCCA GGAAATAACT CCCACCTTGA AAGAACTGAA AACGCAAAGT
    CAGAGTGTGT ATAATAATTT AGTGAGTTTT GCATCACCAT TAGTGACCGA TGCAACAAAT
    GAATGTTCGA GTCCAACATC GTCTGCTACT TATCAACCAT CCTTTGCTGC AGCAGTCCGA
    AGCAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCAC AGAACGCTAG AAAGCTGATT
    CAGAAAAACC TAGCTACATC AGCAGATACC CCACCGAGCA CCGTTCCGGG AACTGGCAAG
    AGTGTTGCTT GTAGTCCTAA AAAGGCCATC AGAGACCCTA AGACTGGAAA AGCTGTGCAG
    GAGAGAAACT CGTATGCGGT GAGTGTGTGG AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA
    GATGTTGATC CGAATAGGAG GATGTCAGTT GCTGAACAGG TTGACTATGT CATTAAGGAA
    GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192844.1
    CDS1..10977
    Translation

    Target ORF information:

    RefSeq Version NM_001205915.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
    CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA
    AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT
    TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT
    GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG
    TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG
    CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT
    TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA
    GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG
    CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA
    AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT
    GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA
    TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT
    GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC
    ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG
    GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA
    GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG
    TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT
    CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG
    TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC
    CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT
    CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT
    GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT
    TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA
    TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT
    TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA
    CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGGTTGTT
    GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG CTCAGTCTGA AGAATATTCC TGTTTTAGAG
    ACTGCCTATA AATTAATTTT GGGAGAAATG ACTTGTGCCC TAAACAATCT GCTACATAGT
    CTACAACTTC CTGATGCCTG TTCTGAAATA AAACATGAGG CTTTTAAGAA TCATGTTTTC
    AATGTAGACA ATGCAAAATT TGTAGTTATA TTTGACCTTA GTGCTCTGAC TACAATTGGA
    AATGCTAAAA ACTCACTAAT TGGGATGTGG GCCCTGTCTC CAACTGTGTT TGCACTTCTG
    AGTAAGAATC TGATGATTGT GCATGGCGAC CTGGCTGTTC ATTTCCCTGC CATTCAGTAT
    GCAGTGCTCT ACACATTGTA TTCTCATTGT ACCAGGCATG ACCACTTTAT ATCTAGTAGT
    CTTAGTTCAT CATCCCCGTC CTTGTTTGAT GGTGCCGTTA TTAGTACTGT AACTACAGCT
    ACAAAAAAAC ATTTCTCAAT TATCTTAAAT CTCCTGGGAA TATTGCTTAA GAAAGATAAT
    CTTAACCAGG ACACAAGGAA ACTGTTAATG ACCTGGGCTT TGGAAGTGGC TGTTTTAATG
    AAGAAGTCTG AAACATATGC ACCTTTATTC TCTCTTCCAT CTTTCCATAA ATTTTGCAAA
    GGCCTTTTAG CCAACACTCT GGTCGAAGAT GTGAATATCT GTCTGCAAGC ATGCAGCAGT
    CTACATGCAC TGTCCTCTTC CTTACCAGAT GATCTTTTAC AGAGGTGTGT TGATGTTTGC
    CGTGTTCAGC TAGTCCATAG TGGAACTCGT ATTCGACAAG CATTTGGAAA GCTATTGAAG
    TCAATCCCTT TAGATGTTGT CTTGAGCAAT AACAATCACA CAGAAATTCA AGAAATTTCT
    TTAGCATTAA GAAGTCACAT GAGTAAAGCA CCAAGTAACA CATTCCACCC ACAAGATTTC
    TCTGATGTTA TTAGTTTTAT TTTGTATGGA AACTCTCATA GAATAGGGAA GGACAATTGG
    TTAGAAAGAT TGTTCTATAG CTGCCAGAGA CTGGATAAAC GTGACCAATC AACGATTCCC
    CGCAATCTGC TAAAGACAGA TGCTGTCCTC TGGCAGTGGG CTATTTGGGA AGCAGCACAG
    TTCACTGTCC TTTCTAAGCT GAGGACCCCA CTTGGCAGAG CTCAGGATAC CTTCCAAACA
    ATTGAAGGTA TCATTAGAAG TCTTGCAGCT CACACATTAA ACCCCGATCA AGATGTTAGT
    CAGTGGACAA CTGCAGACAA TGACGAAGGC CACAGTAGCA ACCAGCTTAG ACTTGTGCTG
    CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG TTAATGTATA ATGCATATGA AGGATGTGCC
    AATGCATTAA CATCGCCTCC CAAGGTCATT AGGACTTTTT TCTATACCAA TCGCCAAACT
    TGTCAAGACT GGCTGACTCG GATCCGACTC TCCATCATGA GGGTTGGATT GTTGGCAGGC
    CAGCCTGCTG TGACAGTGAG ACACGGCTTC GATTTGCTTA CAGAAATGAA GACAAATCCG
    TCTCAGGGAA ATGAATTGGA AGTAACTATC ATGATGGTGG TAGAAGCATT GTGTGAACTT
    CATTGTCCTG AAGCTATACA GGGAATTGCT GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT
    CTTCTCTGGA TTAATTCAGT GGCCCAACAG GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG
    GAGTACCAGG AGCACCTGTG TGCCATGACA GGTGTCGATT GCTGCATCTC TAGCTTCGAC
    AAATCCGTTC TCACATTGGC TAATACTGGG CGTAATAGTG CCAGCCCCAA GCATTCTTTG
    AATGGTGAAT CCAGAAAAAC TGTGCTTTCC AAACCAACTG ATTCTTCTCC TGAGGTTATA
    AATTATTTGG GAAATAAAGC ATGTGAGTGC TACATTTCAA TAGCTGATTG GGCTGCTGTA
    CAGGAGTGGC AGAATGCTGT CCATGACTTG AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC
    CTAAAGGCTG ATTTCAACTA CATAAAATCA CTAAGCAGTT TTGAATCTGG AGAATTTATT
    GAATGCACTG AACAATTAGA ATTGTTACCA GGAGAAAATA TCAGTCTACT TGCTGGAGGA
    TCAAAAGAAA AAATAGATAT GAAAAAACTG CTTCCTAACA TGTTAAGTCC AGATCCAAGA
    GAACTTCAGA AATCCATTGA AGTTCAGTTG TTAAGAAGTT CTGTTTGTTT GGCAACTGCT
    TTAAACCACA TAGAACAAGA TCAGAAGTGG CAGTCTATTA CCGAAAATGT GGTGAAGTAC
    CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA CCCCTGAGAC TTTCTACTCT AACAGTTTCC
    CAGTCTCTGC CAGTCCTAAG TACTTTACAG CTGTATTGCT CTTCTGCTTT GGAGAACACA
    GTTTCCAACA GACTTGCCAC AGAGGACTGT CTTATTCCAC TCTTTAGTGA AGCTTTGCGT
    TCATGTAAAC AGCATGATGT CAGGCCATGG ATGCAAGCAT TAAGATACAC GATGTACCAG
    AGTCAGTTGT TGGAGAAAAT AAAAGAACAA ACAGTTCCAA TTAGAAGCCA TCTCATGGAA
    TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA AAACGGGGGA ATGTGTCACT TGCAACAAGA
    TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG GGAAAGACTA CCACTGCACA GGATTTAGTC
    CAGCACTTTA AAAAACTATC AACTCAAGGT CAAGTGGATG AAAAATGGGG GCCTGAACTT
    GACATTGAAA AAACGAAACT GCTATATACA GCAGGCCAGT CAACACATGC AATGGAGATG
    TTGAGTTCCT GTGCCATCTC TTTCTGCAAG TCTGCCAAAG CTGAGCATGC TGTTGCCAAG
    TCAATTTTGA CATTGGCTAA ATGGATCCAG GCAGAATGGA AAGAAATCTC AGGACAGCTG
    AAACAGGTTT ACAGAGCTCA GCAGCAGCAG AACTTCACGG GTCTTTCTAC TTTGTCTAAA
    AACATACTCA CTCTAATTGA ACTACCATCT GTTAATACGA TGGAGGAAGA GTATCCTCGG
    ATAGAGAGTG AATCTACAGT ACATATTGGA GTTGGGGAAC CTGACTTCAT CTTGGGACAG
    CTGTATCATC TGTCTTCAGT ACAGGCACCT GAAGTAGCCA AATCTTGGGC CGCATTGGCT
    AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG GTTGACAATG CCAGTCAGGG AGAAGGTGTT
    CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT CAGAATCTGC TCCCAGACAC CATAACCGAA
    GAAGAAAAAG AGAGAATATA TGGTATTCTT GGTCAGGCTG TGTGTCGCCC AGCGGGGATT
    CAGGATGAAG ATATAACGCT TCAGATCACA GAAAGTGAAG ATAATGAGGA AGATGACATG
    GTTGATGTTA TCTGGCGTCA GTTAATATCA AGTTGCCCAT GGCTTTCAGA ACTTGATGAA
    AGTGCAACAG AAGGAGTCAT TAAAGTGTGG AGGAAAGTTG TGGACAGAAT CTTCAGCCTG
    TATAAACTGT CCTGCAGCGC ATATTTTACT TTCCTTAAAC TCAATGCTGG GCAGATTCCT
    TTAGATGAAG ATGACCCTCG GCTCCATTTA AGCCACAGAG TGGAGCAGAG CACGGATGAC
    ATGATTGTGA TGGCCACGCT GCGGTTGCTC AGGTTGCTCG TGAAGCACGC TGGGGAGCTT
    CGGCAGTATC TGGAACATGG CTTGGAAACA ACACCTACTG CGCCTTGGAG AGGAATTATT
    CCGCAGCTTT TCTCACGCTT AAACCACCCT GAAGTGTATG TGCGCCAGAG TATTTGTAAC
    CTCCTCTGCC GCGTGGCTCA GGATTCCCCG CATCTCATAC TGTATCCTGC AATAGTGGGC
    ACCATATCGC TTAGTAGTGA GTCACAGGCT TCAGGAAATA AATTTTCCTC TGCAATTCCA
    ACTTTACTTG GCAATATTCA AGGAGAAGAA CTACTGGTTT CTGAATGTGA GGGAGGAAGT
    CCTCCTGCTT CTCAAGATAG CAATAAGGAT GAACCTAAAA GTGGACTGAA TGAAGACCAA
    GCCATGATGC AGGACTGCTA CAGCAAAATT GTAGATAAAC TGTCCTCGGC AAACCCAACT
    ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT GAGCTGCGCA GGGTCACTGT TCTCTGGGAT
    GAGCTCTGGT TGGGAGTTCT GTTACAGCAG CACATGTATG TCCTGAGACG AATTCAGCAG
    TTGGAAGACG AGGTGAAGAG AGTCCAGAAC AACAACACCT TACGCAAAGA AGAGAAAATC
    GCAATCATGC GGGAGAAGCA CACAGCTTTG ATGAAGCCCA TCGTATTTGC TTTGGAGCAC
    GTTAGGAGCA TCACAGCGGC CCCTGCAGAA ACACCTCATG AAAAGTGGTT TCAGGATAAC
    TATGGTGATG CCATTGAAAA TGCTCTAGAA AAGCTGAAAA CACCGTCAAA CCCTGCAAAG
    CCTGGAAGCA GCTGGATTCC ATTTAAAGAG ATAATGCTGA GTTTGCAACA GAGAGCACAG
    AAACGTGCAA GTTACATCTT GCGTCTTGAA GAAATCAGCC CATGGCTGGC TGCCATGACT
    AACACTGAAA TTGCTCTTCC CGGAGAAGTC TCAGCCAGAG ATACCGTCAC AATCCACAGT
    GTTGGTGGAA CCATCACAAT CTTACCTACT AAAACCAAGC CAAAGAAACT TCTCTTTCTA
    GGATCAGATG GGAAGAGCTA TCCTTATCTT TTCAAAGGAT TGGAGGATTT ACATCTGGAT
    GAGAGAATAA TGCAATTTCT GTCTATTGTA AATACCATGT TTGCAACAAT CAATCGCCAG
    GAAACACCTC GTTTCCATGC TCGCCACTAT TCTGTAACAC CACTGGGAAC TCGCTCAGGC
    CTAATCCAGT GGGTGGATGG AGCCACGCCC TTATTTGGTC TTTACAAACG ATGGCAACAA
    CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC CAAGATTCCT ACCAAACTCC TCAGAATCCT
    GGAATCGTAC CCCGTCCTAG TGAACTTTAT TATAGTAAAA TTGGTCCTGC TTTGAAAGCA
    GTTGGGCTTA GTCTGGATGT GTCCCGCCGG GATTGGCCTC TTCATGTGAT GAAGGCAGTG
    TTAGAAGAGT TAATGGAGGC CACGCCCCCA AATCTCCTTG CTAAAGAGCT TTGGTCATCA
    TGTACCACCC CTGATGAGTG GTGGAGAGTC ACACAGTCTT ATGCCAGATC TACTGCAGTC
    ATGTCTATGG TCGGATACAT AATTGGCCTT GGAGATAGAC ATCTGGATAA TGTTCTCATA
    GATATGACGA CTGGAGAGGT TGTTCACATA GATTACAATG TTTGCTTTGA AAAAGGTAAA
    AGCCTTAGAG TTCCTGAGAA AGTACCTTTT CGAATGACAC AGAACATTGA AACAGCACTG
    GGTGTAACTG GAGTAGAAGG TGTTTTTAGG CTTTCATGTG AGCAGGTTCT ACACATTATG
    CGGCGTGGCA GAGAGACTCT GCTGACGCTG CTGGAGGCCT TTGTATATGA CCCCCTGGTG
    GACTGGACAG CTGGGGGCGA GGCTGGGTTT GCTGGTGCGG TCTATGGTGG AGGGGGCCAG
    CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG ATGGAACGAG AGATTACTCG CAGCCTTTTT
    TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC TGGTTTAAAA ACAGAGATGA GATGCTGGTT
    GTACTTCCCA AGCTGGACAG CAGCTTAGAT GAATACCTGA GCTTGCAAGA GCAACTAACA
    GATGTGGAGA AACTGCAGGG CAAACTGCTG GAAGAAATTG AGTTTCTAGA AGGAGCTGAA
    GGAGTGGATC ATCCTTCTCA TACTCTGCAA CACAGGTATT CTGAACACAC TCAACTACAG
    ACTCAGCAGA GAGCTGTTCA GGAAGCAATC CAGGTGAAGC TGAATGAATT TGAACAGTGG
    ATAACACACT ATCAGGCTGC GTTCAATAAT TTGGAAGCTA CACAGCTGGC CAGTTTGCTT
    CAGGAGATAA GCACACAGAT GGACCTTGGT CCTCCCAGCT ACGTGCCGGC AACAGCCTTT
    CTGCAGAATG CTGGCCAGGC CCACTTGATT AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT
    GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG CTGCGAGGCT GTCTGGAGCA GCTGCATCAC
    TACGCAACTG TTGCTCTGCA GTATCCAAAG GCCATATTTC AGAAACATCG AATTGAGCAG
    TGGAAGACCT GGATGGAAGA GCTCATCTGT AATACCACAG TAGAACGCTG TCAGGAACTT
    TACAGGAAAT ATGAAATGCA GTATGCGCCC CAGCCACCCC CAACAGTGTG TCAGTTCATC
    ACGGCCACTG AGATGACCCT GCAGCGGTAC GCAGCCGACA TCAACAGCCG ACTTATCAGA
    CAGGTGGAAC GCTTGAAACA GGAAGCTGTC ACTGTACCGG TTTGTGAAGA TCAGTTGAAA
    GAAATTGAAC GTTGTATCAA AGTTTTCCTT CACGAGAATG GAGAGGAAGG ATCTTTGAGT
    CTAGCAAGTG TTATTATTTC TGCCCTTTGT ACTCTCACAA GGCGTAACCT AATGATGGAA
    GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG GTTGATCTGA CTTCTCGGGA TGGAGCCTGG
    TTCTTGGAGG AACTCTGCAG TATGAGCGGA AATGTCACAT GTCTGGTTCA GTTACTGAAG
    CAGTGTCATT TAGTGCCACA AGACTTAGAC ATTCCTAACC CCATGGAAGC ATCTGAGGCC
    GTTCATTTGG CTAATGGTGT ATACACCTCA CTTCAGGAAT TAAATTCAAA TTTCCGGCAA
    ATCATATTTC CAGAAGCACT GAGATGTTTA ATGAAAGGAG AATACACGTT AGAAAATATG
    CTTCATGAGC TGGACAGTCT TATTGAGCAG ACAACTGATG GTGTTCCTCT GCAGACTCTA
    GTTGAATCTC TTCAAGCCTA TTTAAGAAAT GCAGCTATGG GACTTGAGGA AGAAACACAT
    GCTCATTACA TCGATGTTGC CAGAATGCTT CATGCTCAGT ATGGTGAATT AATCCAACCA
    AGAAATGGTT CAGTTGATGA AACACCAAAA ATGTCAGCTG GCCAGATGCT TTTGGTAGCA
    TTTGATGGCA TGTTTGCCCA AGTTGAGACT GCTTTTGGCT TATTGGTTGA AAAGCTAAAT
    AAGATGGAAA TTCCTATAGC TTGGCGAAAG ATTGACATAA TACGGGAAGC CAGGAGCACT
    CAGGTCAATT TTTTTGATGA TGATAATCAC CGACAGGTGC TGGAAGAGAT CTTCTTTCTA
    AAACGACTAC AGACAATTAA GGAGTTCTTT CGGCTCTGTG GTACCTTTTC TAAAACTTTG
    TCAGGATCAA GTTCACTTGA AGATCAGAAT ACTGTAAATG GGCCTGTACA GATTGTCAAT
    GTGAAAACCC TTTTTAGAAA CTCTTGTTTC AGTGAAGACC AGATGGCCAA ACCAATAAAG
    GCATTCACAG CTGACTTTGT GCGGCAGCTC CTGATTGGAC TCCCGAACCA AGCCCTGGGC
    CTCACACTGT GCAGCTTCAT CAGTGCTCTA GGGGTAGACA TCATTGCGCA GGTGGAGGCG
    AAGGATTTTG GTGCTGAAAG CAAAGTTTCT GTTGACGATC TGTGTAAGAA GGCGGTGGAG
    CACAACATCC AGATCGGGAA GTTCTCTCAG CTGGTCATGA ACAGGGCGAC TGTGCTGGCG
    AGCTCCTACG ACACTGCCTG GAAGAAGCAC GACTTGGTGC GCAGGCTGGA GACCAGCATC
    GCCTCCTGCA AGACAAGCCT GCAGCGTGTC CAGCTGCACA TCGCCATGTT TCAGTGGCAG
    CATGAAGATC TCCTTATCAA TAGACCACAA GCCATGTCTG TCACACCTCC CCCTCGTTCT
    GCCATATTAA CCAGCATGAA AAAGAAACTC CATACTCTGA GCCAGATTGA AACTTCAATT
    GCAACAGTTC AGGAGAAACT GGCAGCACTT GAAGCAAGTA TTGAACAGCG ACTCAAATGG
    GCAGGTGGTG CCAACCCTGC ACTGGCACCT GTACTACAGG ATTTTGAAGC AACAATAGCT
    GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC
    AGCAATATCA TTCATTTTGA AAGTTTACGA ACTAGAACTG CAGAAGCCTT AAACCTGGAT
    GCTGCATTAT TTGAACTAAT CAAACGGTGT CAGCAAATGT GTTCATTTGC ATCGCAGTTT
    AACAGTTCAG TCTCTGAGTT AGAGCTTCGT TTATTACAGA GGGTGGACAC GACTCTTGAA
    CATCCTATTG GCAGCTCTGA GTGGCTTTTG TCAGCACACA AACAACTGAC CCAGGATATG
    TCTACTCAGA GAGCAATTCA GACAGAGAAA GAGCAACAAA TAGAAACAGT CTGTGAAACA
    ATTCAGAATC TGGTTGATAA TATAAAGACT GTGCTCACTG GCCATAACCG GCAGCTTGGA
    GATGTCAAAC ATCTCCTGAA AGCAATGGCC AAGGATGAAG AAGCTGCTCT GGCAGATGGT
    GAAGATGTTC CCTATGAGAA CAGTGTTAGG CAGTTCTTGG GTGAATATAA ATCATGGCAA
    GACAACATTC AGACAGTGCT GTTTACATTA GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA
    GAACATGTTG AAATGCTCCA GGAAATAACT CCCACCTTGA AAGAACTGAA AACGCAAAGT
    CAGAGTGTGT ATAATAATTT AGTGAGTTTT GCATCACCAT TAGTGACCGA TGCAACAAAT
    GAATGTTCGA GTCCAACATC GTCTGCTACT TATCAACCAT CCTTTGCTGC AGCAGTCCGA
    AGCAACACTG GCCAGAAGAC TCAGCCTGAT GTCATGTCAC AGAACGCTAG AAAGCTGATT
    CAGAAAAACC TAGCTACATC AGCAGATACC CCACCGAGCA CCGTTCCGGG AACTGGCAAG
    AGTGTTGCTT GTAGTCCTAA AAAGGCCATC AGAGACCCTA AGACTGGAAA AGCTGTGCAG
    GAGAGAAACT CGTATGCGGT GAGTGTGTGG AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA
    GATGTTGATC CGAATAGGAG GATGTCAGTT GCTGAACAGG TTGACTATGT CATTAAGGAA
    GCAACTAATC TAGATAACTT GGCTCAGCTG TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230306
    Clone ID Related Accession (Same CDS sequence) XM_024984919.1
    Accession Version XM_024984919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase SMG1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
    CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA
    AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT
    TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT
    GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG
    TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG
    CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT
    TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA
    GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG
    CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA
    AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT
    GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA
    TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT
    GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC
    ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG
    GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA
    GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG
    TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT
    CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG
    TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC
    CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT
    CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT
    GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT
    TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA
    TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT
    TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA
    CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGATGTGG
    GCCCTGTCTC CAACTGTGTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCATGGCGAC
    CTGGCTGTTC ATTTCCCTGC CATTCAGTAT GCAGTGCTCT ACACATTGTA TTCTCATTGT
    ACCAGGCATG ACCACTTTAT ATCTAGTAGT CTTAGTTCAT CATCCCCGTC CTTGTTTGAT
    GGTGCCGTTA TTAGTACTGT AACTACAGCT ACAAAAAAAC ATTTCTCAAT TATCTTAAAT
    CTCCTGGGAA TATTGCTTAA GAAAGATAAT CTTAACCAGG ACACAAGGAA ACTGTTAATG
    ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATATGC ACCTTTATTC
    TCTCTTCCAT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT GGTCGAAGAT
    GTGAATATCT GTCTGCAAGC ATGCAGCAGT CTACATGCAC TGTCCTCTTC CTTACCAGAT
    GATCTTTTAC AGAGGTGTGT TGATGTTTGC CGTGTTCAGC TAGTCCATAG TGGAACTCGT
    ATTCGACAAG CATTTGGAAA GCTATTGAAG TCAATCCCTT TAGATGTTGT CTTGAGCAAT
    AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA
    CCAAGTAACA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGA
    AACTCTCATA GAATAGGGAA GGACAATTGG TTAGAAAGAT TGTTCTATAG CTGCCAGAGA
    CTGGATAAAC GTGACCAATC AACGATTCCC CGCAATCTGC TAAAGACAGA TGCTGTCCTC
    TGGCAGTGGG CTATTTGGGA AGCAGCACAG TTCACTGTCC TTTCTAAGCT GAGGACCCCA
    CTTGGCAGAG CTCAGGATAC CTTCCAAACA ATTGAAGGTA TCATTAGAAG TCTTGCAGCT
    CACACATTAA ACCCCGATCA AGATGTTAGT CAGTGGACAA CTGCAGACAA TGACGAAGGC
    CACAGTAGCA ACCAGCTTAG ACTTGTGCTG CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG
    TTAATGTATA ATGCATATGA AGGATGTGCC AATGCATTAA CATCGCCTCC CAAGGTCATT
    AGGACTTTTT TCTATACCAA TCGCCAAACT TGTCAAGACT GGCTGACTCG GATCCGACTC
    TCCATCATGA GGGTTGGATT GTTGGCAGGC CAGCCTGCTG TGACAGTGAG ACACGGCTTC
    GATTTGCTTA CAGAAATGAA GACAAATCCG TCTCAGGGAA ATGAATTGGA AGTAACTATC
    ATGATGGTGG TAGAAGCATT GTGTGAACTT CATTGTCCTG AAGCTATACA GGGAATTGCT
    GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT CTTCTCTGGA TTAATTCAGT GGCCCAACAG
    GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG GAGTACCAGG AGCACCTGTG TGCCATGACA
    GGTGTCGATT GCTGCATCTC TAGCTTCGAC AAATCCGTTC TCACATTGGC TAATACTGGG
    CGTAATAGTG CCAGCCCCAA GCATTCTTTG AATGGTGAAT CCAGAAAAAC TGTGCTTTCC
    AAACCAACTG ATTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC
    TACATTTCAA TAGCTGATTG GGCTGCTGTA CAGGAGTGGC AGAATGCTGT CCATGACTTG
    AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC CTAAAGGCTG ATTTCAACTA CATAAAATCA
    CTAAGCAGTT TTGAATCTGG AGAATTTATT GAATGCACTG AACAATTAGA ATTGTTACCA
    GGAGAAAATA TCAGTCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTG
    CTTCCTAACA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATTGA AGTTCAGTTG
    TTAAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCACA TAGAACAAGA TCAGAAGTGG
    CAGTCTATTA CCGAAAATGT GGTGAAGTAC CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA
    CCCCTGAGAC TTTCTACTCT AACAGTTTCC CAGTCTCTGC CAGTCCTAAG TACTTTACAG
    CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTGCCAC AGAGGACTGT
    CTTATTCCAC TCTTTAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG
    ATGCAAGCAT TAAGATACAC GATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA
    ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA
    AAACGGGGGA ATGTGTCACT TGCAACAAGA TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG
    GGAAAGACTA CCACTGCACA GGATTTAGTC CAGCACTTTA AAAAACTATC AACTCAAGGT
    CAAGTGGATG AAAAATGGGG GCCTGAACTT GACATTGAAA AAACGAAACT GCTATATACA
    GCAGGCCAGT CAACACATGC AATGGAGATG TTGAGTTCCT GTGCCATCTC TTTCTGCAAG
    TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCAATTTTGA CATTGGCTAA ATGGATCCAG
    GCAGAATGGA AAGAAATCTC AGGACAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAG
    AACTTCACGG GTCTTTCTAC TTTGTCTAAA AACATACTCA CTCTAATTGA ACTACCATCT
    GTTAATACGA TGGAGGAAGA GTATCCTCGG ATAGAGAGTG AATCTACAGT ACATATTGGA
    GTTGGGGAAC CTGACTTCAT CTTGGGACAG CTGTATCATC TGTCTTCAGT ACAGGCACCT
    GAAGTAGCCA AATCTTGGGC CGCATTGGCT AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG
    GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT
    CAGAATCTGC TCCCAGACAC CATAACCGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT
    GGTCAGGCTG TGTGTCGCCC AGCGGGGATT CAGGATGAAG ATATAACGCT TCAGATCACA
    GAAAGTGAAG ATAATGAGGA AGATGACATG GTTGATGTTA TCTGGCGTCA GTTAATATCA
    AGTTGCCCAT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGAGTCAT TAAAGTGTGG
    AGGAAAGTTG TGGACAGAAT CTTCAGCCTG TATAAACTGT CCTGCAGCGC ATATTTTACT
    TTCCTTAAAC TCAATGCTGG GCAGATTCCT TTAGATGAAG ATGACCCTCG GCTCCATTTA
    AGCCACAGAG TGGAGCAGAG CACGGATGAC ATGATTGTGA TGGCCACGCT GCGGTTGCTC
    AGGTTGCTCG TGAAGCACGC TGGGGAGCTT CGGCAGTATC TGGAACATGG CTTGGAAACA
    ACACCTACTG CGCCTTGGAG AGGAATTATT CCGCAGCTTT TCTCACGCTT AAACCACCCT
    GAAGTGTATG TGCGCCAGAG TATTTGTAAC CTCCTCTGCC GCGTGGCTCA GGATTCCCCG
    CATCTCATAC TGTATCCTGC AATAGTGGGC ACCATATCGC TTAGTAGTGA GTCACAGGCT
    TCAGGAAATA AATTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA
    CTACTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CTCAAGATAG CAATAAGGAT
    GAACCTAAAA GTGGACTGAA TGAAGACCAA GCCATGATGC AGGACTGCTA CAGCAAAATT
    GTAGATAAAC TGTCCTCGGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT
    GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG
    CACATGTATG TCCTGAGACG AATTCAGCAG TTGGAAGACG AGGTGAAGAG AGTCCAGAAC
    AACAACACCT TACGCAAAGA AGAGAAAATC GCAATCATGC GGGAGAAGCA CACAGCTTTG
    ATGAAGCCCA TCGTATTTGC TTTGGAGCAC GTTAGGAGCA TCACAGCGGC CCCTGCAGAA
    ACACCTCATG AAAAGTGGTT TCAGGATAAC TATGGTGATG CCATTGAAAA TGCTCTAGAA
    AAGCTGAAAA CACCGTCAAA CCCTGCAAAG CCTGGAAGCA GCTGGATTCC ATTTAAAGAG
    ATAATGCTGA GTTTGCAACA GAGAGCACAG AAACGTGCAA GTTACATCTT GCGTCTTGAA
    GAAATCAGCC CATGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC CGGAGAAGTC
    TCAGCCAGAG ATACCGTCAC AATCCACAGT GTTGGTGGAA CCATCACAAT CTTACCTACT
    AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCAGATG GGAAGAGCTA TCCTTATCTT
    TTCAAAGGAT TGGAGGATTT ACATCTGGAT GAGAGAATAA TGCAATTTCT GTCTATTGTA
    AATACCATGT TTGCAACAAT CAATCGCCAG GAAACACCTC GTTTCCATGC TCGCCACTAT
    TCTGTAACAC CACTGGGAAC TCGCTCAGGC CTAATCCAGT GGGTGGATGG AGCCACGCCC
    TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC
    CAAGATTCCT ACCAAACTCC TCAGAATCCT GGAATCGTAC CCCGTCCTAG TGAACTTTAT
    TATAGTAAAA TTGGTCCTGC TTTGAAAGCA GTTGGGCTTA GTCTGGATGT GTCCCGCCGG
    GATTGGCCTC TTCATGTGAT GAAGGCAGTG TTAGAAGAGT TAATGGAGGC CACGCCCCCA
    AATCTCCTTG CTAAAGAGCT TTGGTCATCA TGTACCACCC CTGATGAGTG GTGGAGAGTC
    ACACAGTCTT ATGCCAGATC TACTGCAGTC ATGTCTATGG TCGGATACAT AATTGGCCTT
    GGAGATAGAC ATCTGGATAA TGTTCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA
    GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTACCTTTT
    CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGG
    CTTTCATGTG AGCAGGTTCT ACACATTATG CGGCGTGGCA GAGAGACTCT GCTGACGCTG
    CTGGAGGCCT TTGTATATGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT
    GCTGGTGCGG TCTATGGTGG AGGGGGCCAG CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG
    ATGGAACGAG AGATTACTCG CAGCCTTTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC
    TGGTTTAAAA ACAGAGATGA GATGCTGGTT GTACTTCCCA AGCTGGACAG CAGCTTAGAT
    GAATACCTGA GCTTGCAAGA GCAACTAACA GATGTGGAGA AACTGCAGGG CAAACTGCTG
    GAAGAAATTG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA
    CACAGGTATT CTGAACACAC TCAACTACAG ACTCAGCAGA GAGCTGTTCA GGAAGCAATC
    CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACACT ATCAGGCTGC GTTCAATAAT
    TTGGAAGCTA CACAGCTGGC CAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTTGGT
    CCTCCCAGCT ACGTGCCGGC AACAGCCTTT CTGCAGAATG CTGGCCAGGC CCACTTGATT
    AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG
    CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TACGCAACTG TTGCTCTGCA GTATCCAAAG
    GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA GCTCATCTGT
    AATACCACAG TAGAACGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCGCCC
    CAGCCACCCC CAACAGTGTG TCAGTTCATC ACGGCCACTG AGATGACCCT GCAGCGGTAC
    GCAGCCGACA TCAACAGCCG ACTTATCAGA CAGGTGGAAC GCTTGAAACA GGAAGCTGTC
    ACTGTACCGG TTTGTGAAGA TCAGTTGAAA GAAATTGAAC GTTGTATCAA AGTTTTCCTT
    CACGAGAATG GAGAGGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATTTC TGCCCTTTGT
    ACTCTCACAA GGCGTAACCT AATGATGGAA GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG
    GTTGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AACTCTGCAG TATGAGCGGA
    AATGTCACAT GTCTGGTTCA GTTACTGAAG CAGTGTCATT TAGTGCCACA AGACTTAGAC
    ATTCCTAACC CCATGGAAGC ATCTGAGGCC GTTCATTTGG CTAATGGTGT ATACACCTCA
    CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA
    ATGAAAGGAG AATACACGTT AGAAAATATG CTTCATGAGC TGGACAGTCT TATTGAGCAG
    ACAACTGATG GTGTTCCTCT GCAGACTCTA GTTGAATCTC TTCAAGCCTA TTTAAGAAAT
    GCAGCTATGG GACTTGAGGA AGAAACACAT GCTCATTACA TCGATGTTGC CAGAATGCTT
    CATGCTCAGT ATGGTGAATT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA
    ATGTCAGCTG GCCAGATGCT TTTGGTAGCA TTTGATGGCA TGTTTGCCCA AGTTGAGACT
    GCTTTTGGCT TATTGGTTGA AAAGCTAAAT AAGATGGAAA TTCCTATAGC TTGGCGAAAG
    ATTGACATAA TACGGGAAGC CAGGAGCACT CAGGTCAATT TTTTTGATGA TGATAATCAC
    CGACAGGTGC TGGAAGAGAT CTTCTTTCTA AAACGACTAC AGACAATTAA GGAGTTCTTT
    CGGCTCTGTG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT
    ACTGTAAATG GGCCTGTACA GATTGTCAAT GTGAAAACCC TTTTTAGAAA CTCTTGTTTC
    AGTGAAGACC AGATGGCCAA ACCAATAAAG GCATTCACAG CTGACTTTGT GCGGCAGCTC
    CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACACTGT GCAGCTTCAT CAGTGCTCTA
    GGGGTAGACA TCATTGCGCA GGTGGAGGCG AAGGATTTTG GTGCTGAAAG CAAAGTTTCT
    GTTGACGATC TGTGTAAGAA GGCGGTGGAG CACAACATCC AGATCGGGAA GTTCTCTCAG
    CTGGTCATGA ACAGGGCGAC TGTGCTGGCG AGCTCCTACG ACACTGCCTG GAAGAAGCAC
    GACTTGGTGC GCAGGCTGGA GACCAGCATC GCCTCCTGCA AGACAAGCCT GCAGCGTGTC
    CAGCTGCACA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA TAGACCACAA
    GCCATGTCTG TCACACCTCC CCCTCGTTCT GCCATATTAA CCAGCATGAA AAAGAAACTC
    CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCACTT
    GAAGCAAGTA TTGAACAGCG ACTCAAATGG GCAGGTGGTG CCAACCCTGC ACTGGCACCT
    GTACTACAGG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC
    CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC AGCAATATCA TTCATTTTGA AAGTTTACGA
    ACTAGAACTG CAGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAACTAAT CAAACGGTGT
    CAGCAAATGT GTTCATTTGC ATCGCAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT
    TTATTACAGA GGGTGGACAC GACTCTTGAA CATCCTATTG GCAGCTCTGA GTGGCTTTTG
    TCAGCACACA AACAACTGAC CCAGGATATG TCTACTCAGA GAGCAATTCA GACAGAGAAA
    GAGCAACAAA TAGAAACAGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT
    GTGCTCACTG GCCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC
    AAGGATGAAG AAGCTGCTCT GGCAGATGGT GAAGATGTTC CCTATGAGAA CAGTGTTAGG
    CAGTTCTTGG GTGAATATAA ATCATGGCAA GACAACATTC AGACAGTGCT GTTTACATTA
    GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA GAACATGTTG AAATGCTCCA GGAAATAACT
    CCCACCTTGA AAGAACTGAA AACGCAAAGT CAGAGTGTGT ATAATAATTT AGTGAGTTTT
    GCATCACCAT TAGTGACCGA TGCAACAAAT GAATGTTCGA GTCCAACATC GTCTGCTACT
    TATCAACCAT CCTTTGCTGC AGCAGTCCGA AGCAACACTG GCCAGAAGAC TCAGCCTGAT
    GTCATGTCAC AGAACGCTAG AAAGCTGATT CAGAAAAACC TAGCTACATC AGCAGATACC
    CCACCGAGCA CCGTTCCGGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCCATC
    AGAGACCCTA AGACTGGAAA AGCTGTGCAG GAGAGAAACT CGTATGCGGT GAGTGTGTGG
    AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT
    GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG
    TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840687.1
    CDS335..11041
    Translation

    Target ORF information:

    RefSeq Version XM_024984919.1
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG 
    CGGAGCTGGA ATGACTGGCA ACCCAGAACT GATAGTGCAT CAGCCGACCC AGATAATTTA
    AAATACTCTT CATCCAGAGA TAGGGGTGGG TCTTCCTCTT ATGGACTGCA GCCTTCAAAT
    TCAGCTGTGG TGTCTCGGCA AAGGCATGAT GATACCAGAA TCCATGCTGA CATACAGAAT
    GACGAAAAGG GTGGCTACAG TGTCAATGGA GGATCTGGGG AAAATACTTA TGGTCGGAAG
    TCGTTGGGGC AAGAGCTGAG GGTTAACAAT GTGACCAGCC CTGAGTTTAC CAGTATTCAG
    CATGGCAGTC GTGCGTTAGC CATCAAAGAC ATGAGGAAAT CACAGGAGCG ATCGATGTCT
    TATTCTGATG AGTCTCGACT GTCGAATCTT CTTCGGAGGA TCACCCGGGA AGATGACAGA
    GACCGAAGGC TGGCTACTGT TAAGCAGTTG AAAGAATTTA TTCAGCAACC AGAAAATAAG
    CTGGTGCTAG TTAAGCAGTT GGATAATATC TTGGCTGCTG TACATGATGT GCTTAATGAA
    AGTAGCAAAT TGCTTCAGGA GTTGAGACAG GAGGGAGCTT GTTGTCTTGG CCTTCTTTGT
    GCATCTCTGA GCTATGAGGC TGAGAAGATC TTCAAATGGA TTTTTAGCAA ATTTAGCTCA
    TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC TTATGTGCCA CCTACAAAGC ACTAGAGACT
    GTAGGAGAAA AGAAAGCCTT TTCATCTGTA ATGCAGCTTG TAATGACCAG CCTGCAGTCC
    ATTCTTGAAA ATGTGGATAC ACCAGAATTA CTTTGCAAGT GTGTTAAGTG CATTCTTTTG
    GTAGCTCGAT GTTACCCTCA TATTTTCAGC ACAAATTTTA GGGATACAGT TGATATATTA
    GTTGGATGGC ATATAGATCA TACTCAGAAG CCTTCACTTA CACAACAGGT GTCTGGGTGG
    TTGCAGAGTT TGGAGCCATT CTGGGTAGCT GACCTTGCAT TTTCTACCAC TCTTCTTGGT
    CAGTTTTTAG AGGACATGGA GGCATATGCT GAGGACCTCA GCCATGTAGC CTCTGGGGAG
    TCAGTGGATG AAGATGTTCC TCCTCCATCA GTGTCCTTAC CAAAGCTGGC AGCACTTCTC
    CGGGTATTCA GTACTGTGGT GAGAAGCATT GGGGAACGTT TCAGCCCAAT TCGAGGTCCT
    CCAATTACTG AGGCATATGT AACAGATGTT CTGTACAGAG TAATGAGATG TGTGACGGCT
    GCAAACCAAG TGTTTTTTTC TGAGGCTGTG TTGACAGCTG CTAATGAGTG TGTTGGTGTT
    TTGCTCGGCA GCTTGGATCC TAGCATGACT ATACATTGTG ACATGGTTAT TACATATGGA
    TTAGATCAAC TGGAGAATTG CCAGACTTGT GGTACAGATT ATATCATCTC AGTCTTGAAT
    TTACTCACAT TGATTGTTGA ACAGATAAAT ACAAAACTGC CATCATCATT TGTAGAAAAA
    CTGTTTATAC CATCATCTAA GCTACTATTT TTGCGTTATC ATAAAGAAAA AGAGATGTGG
    GCCCTGTCTC CAACTGTGTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCATGGCGAC
    CTGGCTGTTC ATTTCCCTGC CATTCAGTAT GCAGTGCTCT ACACATTGTA TTCTCATTGT
    ACCAGGCATG ACCACTTTAT ATCTAGTAGT CTTAGTTCAT CATCCCCGTC CTTGTTTGAT
    GGTGCCGTTA TTAGTACTGT AACTACAGCT ACAAAAAAAC ATTTCTCAAT TATCTTAAAT
    CTCCTGGGAA TATTGCTTAA GAAAGATAAT CTTAACCAGG ACACAAGGAA ACTGTTAATG
    ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATATGC ACCTTTATTC
    TCTCTTCCAT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT GGTCGAAGAT
    GTGAATATCT GTCTGCAAGC ATGCAGCAGT CTACATGCAC TGTCCTCTTC CTTACCAGAT
    GATCTTTTAC AGAGGTGTGT TGATGTTTGC CGTGTTCAGC TAGTCCATAG TGGAACTCGT
    ATTCGACAAG CATTTGGAAA GCTATTGAAG TCAATCCCTT TAGATGTTGT CTTGAGCAAT
    AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA
    CCAAGTAACA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGA
    AACTCTCATA GAATAGGGAA GGACAATTGG TTAGAAAGAT TGTTCTATAG CTGCCAGAGA
    CTGGATAAAC GTGACCAATC AACGATTCCC CGCAATCTGC TAAAGACAGA TGCTGTCCTC
    TGGCAGTGGG CTATTTGGGA AGCAGCACAG TTCACTGTCC TTTCTAAGCT GAGGACCCCA
    CTTGGCAGAG CTCAGGATAC CTTCCAAACA ATTGAAGGTA TCATTAGAAG TCTTGCAGCT
    CACACATTAA ACCCCGATCA AGATGTTAGT CAGTGGACAA CTGCAGACAA TGACGAAGGC
    CACAGTAGCA ACCAGCTTAG ACTTGTGCTG CTTCTGCAGT ATCTGGAAAA CCTGGAGAAG
    TTAATGTATA ATGCATATGA AGGATGTGCC AATGCATTAA CATCGCCTCC CAAGGTCATT
    AGGACTTTTT TCTATACCAA TCGCCAAACT TGTCAAGACT GGCTGACTCG GATCCGACTC
    TCCATCATGA GGGTTGGATT GTTGGCAGGC CAGCCTGCTG TGACAGTGAG ACACGGCTTC
    GATTTGCTTA CAGAAATGAA GACAAATCCG TCTCAGGGAA ATGAATTGGA AGTAACTATC
    ATGATGGTGG TAGAAGCATT GTGTGAACTT CATTGTCCTG AAGCTATACA GGGAATTGCT
    GTCTGGTCGT CTTCGGTTGT TGGAAAAAAT CTTCTCTGGA TTAATTCAGT GGCCCAACAG
    GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG GAGTACCAGG AGCACCTGTG TGCCATGACA
    GGTGTCGATT GCTGCATCTC TAGCTTCGAC AAATCCGTTC TCACATTGGC TAATACTGGG
    CGTAATAGTG CCAGCCCCAA GCATTCTTTG AATGGTGAAT CCAGAAAAAC TGTGCTTTCC
    AAACCAACTG ATTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC
    TACATTTCAA TAGCTGATTG GGCTGCTGTA CAGGAGTGGC AGAATGCTGT CCATGACTTG
    AAAAAGAGTA CCAGTAGTAC TTCTCTCAAC CTAAAGGCTG ATTTCAACTA CATAAAATCA
    CTAAGCAGTT TTGAATCTGG AGAATTTATT GAATGCACTG AACAATTAGA ATTGTTACCA
    GGAGAAAATA TCAGTCTACT TGCTGGAGGA TCAAAAGAAA AAATAGATAT GAAAAAACTG
    CTTCCTAACA TGTTAAGTCC AGATCCAAGA GAACTTCAGA AATCCATTGA AGTTCAGTTG
    TTAAGAAGTT CTGTTTGTTT GGCAACTGCT TTAAACCACA TAGAACAAGA TCAGAAGTGG
    CAGTCTATTA CCGAAAATGT GGTGAAGTAC CTGAAGCAAA CCTCCCGCAT AGCTGTTGGA
    CCCCTGAGAC TTTCTACTCT AACAGTTTCC CAGTCTCTGC CAGTCCTAAG TACTTTACAG
    CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCCAACA GACTTGCCAC AGAGGACTGT
    CTTATTCCAC TCTTTAGTGA AGCTTTGCGT TCATGTAAAC AGCATGATGT CAGGCCATGG
    ATGCAAGCAT TAAGATACAC GATGTACCAG AGTCAGTTGT TGGAGAAAAT AAAAGAACAA
    ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGTCTAA CAGCAGCAAA ATTTGCTAGA
    AAACGGGGGA ATGTGTCACT TGCAACAAGA TTGCTGGCAC AGTGTAGCGA GGTTCAGCTG
    GGAAAGACTA CCACTGCACA GGATTTAGTC CAGCACTTTA AAAAACTATC AACTCAAGGT
    CAAGTGGATG AAAAATGGGG GCCTGAACTT GACATTGAAA AAACGAAACT GCTATATACA
    GCAGGCCAGT CAACACATGC AATGGAGATG TTGAGTTCCT GTGCCATCTC TTTCTGCAAG
    TCTGCCAAAG CTGAGCATGC TGTTGCCAAG TCAATTTTGA CATTGGCTAA ATGGATCCAG
    GCAGAATGGA AAGAAATCTC AGGACAGCTG AAACAGGTTT ACAGAGCTCA GCAGCAGCAG
    AACTTCACGG GTCTTTCTAC TTTGTCTAAA AACATACTCA CTCTAATTGA ACTACCATCT
    GTTAATACGA TGGAGGAAGA GTATCCTCGG ATAGAGAGTG AATCTACAGT ACATATTGGA
    GTTGGGGAAC CTGACTTCAT CTTGGGACAG CTGTATCATC TGTCTTCAGT ACAGGCACCT
    GAAGTAGCCA AATCTTGGGC CGCATTGGCT AGTTGGGCTT ACAGGTGGGG CAGAAAGGTG
    GTTGACAATG CCAGTCAGGG AGAAGGTGTT CGTCTGTTGC CTAGAGAAAA ATCTGAAGTT
    CAGAATCTGC TCCCAGACAC CATAACCGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT
    GGTCAGGCTG TGTGTCGCCC AGCGGGGATT CAGGATGAAG ATATAACGCT TCAGATCACA
    GAAAGTGAAG ATAATGAGGA AGATGACATG GTTGATGTTA TCTGGCGTCA GTTAATATCA
    AGTTGCCCAT GGCTTTCAGA ACTTGATGAA AGTGCAACAG AAGGAGTCAT TAAAGTGTGG
    AGGAAAGTTG TGGACAGAAT CTTCAGCCTG TATAAACTGT CCTGCAGCGC ATATTTTACT
    TTCCTTAAAC TCAATGCTGG GCAGATTCCT TTAGATGAAG ATGACCCTCG GCTCCATTTA
    AGCCACAGAG TGGAGCAGAG CACGGATGAC ATGATTGTGA TGGCCACGCT GCGGTTGCTC
    AGGTTGCTCG TGAAGCACGC TGGGGAGCTT CGGCAGTATC TGGAACATGG CTTGGAAACA
    ACACCTACTG CGCCTTGGAG AGGAATTATT CCGCAGCTTT TCTCACGCTT AAACCACCCT
    GAAGTGTATG TGCGCCAGAG TATTTGTAAC CTCCTCTGCC GCGTGGCTCA GGATTCCCCG
    CATCTCATAC TGTATCCTGC AATAGTGGGC ACCATATCGC TTAGTAGTGA GTCACAGGCT
    TCAGGAAATA AATTTTCCTC TGCAATTCCA ACTTTACTTG GCAATATTCA AGGAGAAGAA
    CTACTGGTTT CTGAATGTGA GGGAGGAAGT CCTCCTGCTT CTCAAGATAG CAATAAGGAT
    GAACCTAAAA GTGGACTGAA TGAAGACCAA GCCATGATGC AGGACTGCTA CAGCAAAATT
    GTAGATAAAC TGTCCTCGGC AAACCCAACT ATGGTGTTAC AGGTTCAGAT GCTTGTGGCT
    GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAG
    CACATGTATG TCCTGAGACG AATTCAGCAG TTGGAAGACG AGGTGAAGAG AGTCCAGAAC
    AACAACACCT TACGCAAAGA AGAGAAAATC GCAATCATGC GGGAGAAGCA CACAGCTTTG
    ATGAAGCCCA TCGTATTTGC TTTGGAGCAC GTTAGGAGCA TCACAGCGGC CCCTGCAGAA
    ACACCTCATG AAAAGTGGTT TCAGGATAAC TATGGTGATG CCATTGAAAA TGCTCTAGAA
    AAGCTGAAAA CACCGTCAAA CCCTGCAAAG CCTGGAAGCA GCTGGATTCC ATTTAAAGAG
    ATAATGCTGA GTTTGCAACA GAGAGCACAG AAACGTGCAA GTTACATCTT GCGTCTTGAA
    GAAATCAGCC CATGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC CGGAGAAGTC
    TCAGCCAGAG ATACCGTCAC AATCCACAGT GTTGGTGGAA CCATCACAAT CTTACCTACT
    AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGATCAGATG GGAAGAGCTA TCCTTATCTT
    TTCAAAGGAT TGGAGGATTT ACATCTGGAT GAGAGAATAA TGCAATTTCT GTCTATTGTA
    AATACCATGT TTGCAACAAT CAATCGCCAG GAAACACCTC GTTTCCATGC TCGCCACTAT
    TCTGTAACAC CACTGGGAAC TCGCTCAGGC CTAATCCAGT GGGTGGATGG AGCCACGCCC
    TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCCG CCTTGCAAGC ACAAAAGGCC
    CAAGATTCCT ACCAAACTCC TCAGAATCCT GGAATCGTAC CCCGTCCTAG TGAACTTTAT
    TATAGTAAAA TTGGTCCTGC TTTGAAAGCA GTTGGGCTTA GTCTGGATGT GTCCCGCCGG
    GATTGGCCTC TTCATGTGAT GAAGGCAGTG TTAGAAGAGT TAATGGAGGC CACGCCCCCA
    AATCTCCTTG CTAAAGAGCT TTGGTCATCA TGTACCACCC CTGATGAGTG GTGGAGAGTC
    ACACAGTCTT ATGCCAGATC TACTGCAGTC ATGTCTATGG TCGGATACAT AATTGGCCTT
    GGAGATAGAC ATCTGGATAA TGTTCTCATA GATATGACGA CTGGAGAGGT TGTTCACATA
    GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTACCTTTT
    CGAATGACAC AGAACATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGG
    CTTTCATGTG AGCAGGTTCT ACACATTATG CGGCGTGGCA GAGAGACTCT GCTGACGCTG
    CTGGAGGCCT TTGTATATGA CCCCCTGGTG GACTGGACAG CTGGGGGCGA GGCTGGGTTT
    GCTGGTGCGG TCTATGGTGG AGGGGGCCAG CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG
    ATGGAACGAG AGATTACTCG CAGCCTTTTT TCTTCGAGAG TAGCTGAAAT TAAGGTGAAC
    TGGTTTAAAA ACAGAGATGA GATGCTGGTT GTACTTCCCA AGCTGGACAG CAGCTTAGAT
    GAATACCTGA GCTTGCAAGA GCAACTAACA GATGTGGAGA AACTGCAGGG CAAACTGCTG
    GAAGAAATTG AGTTTCTAGA AGGAGCTGAA GGAGTGGATC ATCCTTCTCA TACTCTGCAA
    CACAGGTATT CTGAACACAC TCAACTACAG ACTCAGCAGA GAGCTGTTCA GGAAGCAATC
    CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACACT ATCAGGCTGC GTTCAATAAT
    TTGGAAGCTA CACAGCTGGC CAGTTTGCTT CAGGAGATAA GCACACAGAT GGACCTTGGT
    CCTCCCAGCT ACGTGCCGGC AACAGCCTTT CTGCAGAATG CTGGCCAGGC CCACTTGATT
    AGCCAGTGTG AGCAGCTAGA AGGGGAGGTT GGTGCCCTCC TGCAGCAGAG GCGCTCGGTG
    CTGCGAGGCT GTCTGGAGCA GCTGCATCAC TACGCAACTG TTGCTCTGCA GTATCCAAAG
    GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA GCTCATCTGT
    AATACCACAG TAGAACGCTG TCAGGAACTT TACAGGAAAT ATGAAATGCA GTATGCGCCC
    CAGCCACCCC CAACAGTGTG TCAGTTCATC ACGGCCACTG AGATGACCCT GCAGCGGTAC
    GCAGCCGACA TCAACAGCCG ACTTATCAGA CAGGTGGAAC GCTTGAAACA GGAAGCTGTC
    ACTGTACCGG TTTGTGAAGA TCAGTTGAAA GAAATTGAAC GTTGTATCAA AGTTTTCCTT
    CACGAGAATG GAGAGGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATTTC TGCCCTTTGT
    ACTCTCACAA GGCGTAACCT AATGATGGAA GGTGCAGCGT CGAGTGCTGG AGAGCAGCTG
    GTTGATCTGA CTTCTCGGGA TGGAGCCTGG TTCTTGGAGG AACTCTGCAG TATGAGCGGA
    AATGTCACAT GTCTGGTTCA GTTACTGAAG CAGTGTCATT TAGTGCCACA AGACTTAGAC
    ATTCCTAACC CCATGGAAGC ATCTGAGGCC GTTCATTTGG CTAATGGTGT ATACACCTCA
    CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA
    ATGAAAGGAG AATACACGTT AGAAAATATG CTTCATGAGC TGGACAGTCT TATTGAGCAG
    ACAACTGATG GTGTTCCTCT GCAGACTCTA GTTGAATCTC TTCAAGCCTA TTTAAGAAAT
    GCAGCTATGG GACTTGAGGA AGAAACACAT GCTCATTACA TCGATGTTGC CAGAATGCTT
    CATGCTCAGT ATGGTGAATT AATCCAACCA AGAAATGGTT CAGTTGATGA AACACCAAAA
    ATGTCAGCTG GCCAGATGCT TTTGGTAGCA TTTGATGGCA TGTTTGCCCA AGTTGAGACT
    GCTTTTGGCT TATTGGTTGA AAAGCTAAAT AAGATGGAAA TTCCTATAGC TTGGCGAAAG
    ATTGACATAA TACGGGAAGC CAGGAGCACT CAGGTCAATT TTTTTGATGA TGATAATCAC
    CGACAGGTGC TGGAAGAGAT CTTCTTTCTA AAACGACTAC AGACAATTAA GGAGTTCTTT
    CGGCTCTGTG GTACCTTTTC TAAAACTTTG TCAGGATCAA GTTCACTTGA AGATCAGAAT
    ACTGTAAATG GGCCTGTACA GATTGTCAAT GTGAAAACCC TTTTTAGAAA CTCTTGTTTC
    AGTGAAGACC AGATGGCCAA ACCAATAAAG GCATTCACAG CTGACTTTGT GCGGCAGCTC
    CTGATTGGAC TCCCGAACCA AGCCCTGGGC CTCACACTGT GCAGCTTCAT CAGTGCTCTA
    GGGGTAGACA TCATTGCGCA GGTGGAGGCG AAGGATTTTG GTGCTGAAAG CAAAGTTTCT
    GTTGACGATC TGTGTAAGAA GGCGGTGGAG CACAACATCC AGATCGGGAA GTTCTCTCAG
    CTGGTCATGA ACAGGGCGAC TGTGCTGGCG AGCTCCTACG ACACTGCCTG GAAGAAGCAC
    GACTTGGTGC GCAGGCTGGA GACCAGCATC GCCTCCTGCA AGACAAGCCT GCAGCGTGTC
    CAGCTGCACA TCGCCATGTT TCAGTGGCAG CATGAAGATC TCCTTATCAA TAGACCACAA
    GCCATGTCTG TCACACCTCC CCCTCGTTCT GCCATATTAA CCAGCATGAA AAAGAAACTC
    CATACTCTGA GCCAGATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT GGCAGCACTT
    GAAGCAAGTA TTGAACAGCG ACTCAAATGG GCAGGTGGTG CCAACCCTGC ACTGGCACCT
    GTACTACAGG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAGGAGAGC
    CAAAGAGCAA GTCAGGTCAC TTTCCTCTGC AGCAATATCA TTCATTTTGA AAGTTTACGA
    ACTAGAACTG CAGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAACTAAT CAAACGGTGT
    CAGCAAATGT GTTCATTTGC ATCGCAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT
    TTATTACAGA GGGTGGACAC GACTCTTGAA CATCCTATTG GCAGCTCTGA GTGGCTTTTG
    TCAGCACACA AACAACTGAC CCAGGATATG TCTACTCAGA GAGCAATTCA GACAGAGAAA
    GAGCAACAAA TAGAAACAGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT
    GTGCTCACTG GCCATAACCG GCAGCTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCC
    AAGGATGAAG AAGCTGCTCT GGCAGATGGT GAAGATGTTC CCTATGAGAA CAGTGTTAGG
    CAGTTCTTGG GTGAATATAA ATCATGGCAA GACAACATTC AGACAGTGCT GTTTACATTA
    GTCCAAGCCA TGGGTCAGGT TCGAAGTCAA GAACATGTTG AAATGCTCCA GGAAATAACT
    CCCACCTTGA AAGAACTGAA AACGCAAAGT CAGAGTGTGT ATAATAATTT AGTGAGTTTT
    GCATCACCAT TAGTGACCGA TGCAACAAAT GAATGTTCGA GTCCAACATC GTCTGCTACT
    TATCAACCAT CCTTTGCTGC AGCAGTCCGA AGCAACACTG GCCAGAAGAC TCAGCCTGAT
    GTCATGTCAC AGAACGCTAG AAAGCTGATT CAGAAAAACC TAGCTACATC AGCAGATACC
    CCACCGAGCA CCGTTCCGGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCCATC
    AGAGACCCTA AGACTGGAAA AGCTGTGCAG GAGAGAAACT CGTATGCGGT GAGTGTGTGG
    AAGAGAGTGA AAGCCAAGCT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT
    GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG
    TATGAAGGTT GGACAGCCTG GGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32978
    Clone ID Related Accession (Same CDS sequence) XM_005224744.4 , XM_005224744.3
    Accession Version XM_005224744.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase SMG1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224744.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC
    AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA
    TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT
    TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA
    CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC
    TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT
    CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG
    TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT
    GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG
    CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC
    GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG
    GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT
    CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT
    ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA
    CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT
    CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC
    AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA
    TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG
    CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG
    TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC
    GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT
    AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA
    ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG
    AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA
    AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT
    AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT
    ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG
    AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT
    CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA
    GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG
    ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG
    GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC
    CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC
    ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT
    ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG
    GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG
    GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT
    GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC
    CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA
    GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT
    AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC
    AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG
    CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT
    ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC
    ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA
    AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT
    AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT
    AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC
    ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG
    CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT
    CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG
    AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG
    CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC
    AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC
    AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC
    ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA
    ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT
    TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT
    AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC
    CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG
    TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT
    AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA
    TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG
    GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA
    ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA
    GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC
    AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC
    ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA
    CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC
    GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA
    CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG
    AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC
    ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC
    ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT
    AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT
    GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT
    ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT
    CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC
    ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC
    TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA
    GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC
    GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG
    CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG
    TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA
    AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA
    CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA
    GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA
    CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA
    CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC
    TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA
    CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA
    CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG
    AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC
    CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA
    GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224801.1
    CDS147..11087
    Translation

    Target ORF information:

    RefSeq Version XM_005224744.4
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224744.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC
    AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA
    TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT
    TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA
    CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC
    TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT
    CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG
    TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT
    GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG
    CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC
    GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG
    GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT
    CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT
    ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA
    CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT
    CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC
    AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA
    TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG
    CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG
    TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC
    GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT
    AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA
    ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG
    AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA
    AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT
    AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT
    ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG
    AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT
    CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA
    GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG
    ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG
    GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC
    CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC
    ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT
    ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG
    GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG
    GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT
    GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC
    CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA
    GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT
    AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC
    AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG
    CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT
    ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC
    ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA
    AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT
    AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT
    AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC
    ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG
    CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT
    CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG
    AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG
    CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC
    AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC
    AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC
    ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA
    ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT
    TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT
    AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC
    CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG
    TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT
    AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA
    TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG
    GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA
    ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA
    GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC
    AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC
    ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA
    CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC
    GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA
    CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG
    AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC
    ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC
    ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT
    AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT
    GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT
    ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT
    CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC
    ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC
    TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA
    GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC
    GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG
    CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG
    TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA
    AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA
    CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA
    GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA
    CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA
    CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC
    TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA
    CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA
    CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG
    AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC
    CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA
    GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32978
    Clone ID Related Accession (Same CDS sequence) XM_005224744.4 , XM_005224744.3
    Accession Version XM_005224744.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase SMG1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
    3661
    3721
    3781
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    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    7021
    7081
    7141
    7201
    7261
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    7381
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    7501
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    7741
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC
    AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA
    TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT
    TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA
    CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC
    TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT
    CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG
    TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT
    GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG
    CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC
    GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG
    GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT
    CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT
    ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA
    CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT
    CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC
    AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA
    TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG
    CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG
    TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC
    GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT
    AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA
    ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG
    AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA
    AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT
    AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT
    ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG
    AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT
    CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA
    GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG
    ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG
    GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC
    CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC
    ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT
    ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG
    GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG
    GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT
    GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC
    CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA
    GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT
    AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC
    AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG
    CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT
    ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC
    ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA
    AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT
    AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT
    AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC
    ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG
    CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT
    CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG
    AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG
    CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC
    AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC
    AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC
    ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA
    ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT
    TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT
    AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC
    CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG
    TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT
    AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA
    TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG
    GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA
    ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA
    GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC
    AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC
    ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA
    CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC
    GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA
    CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG
    AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC
    ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC
    ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT
    AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT
    GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT
    ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT
    CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC
    ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC
    TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA
    GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC
    GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG
    CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG
    TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA
    AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA
    CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA
    GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA
    CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA
    CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC
    TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA
    CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA
    CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG
    AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC
    CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA
    GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224801.1
    CDS114..11054
    Translation

    Target ORF information:

    RefSeq Version XM_005224744.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224744.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGCAAAAAC ATCACAAAGG CTCCATATTA TACAGTATGC GATTTTTGAG AACTGATAGT 
    GCATCAGCCG ACCCAGATAA TTTAAAATAC TCTTCATCCA GAGATAGGGG TGGGTCTTCC
    TCTTATGGAC TGCAGCCTTC AAATTCAGCT GTGGTGTCTC GGCAAAGGCA TGATGATACC
    AGAATCCATG CTGACATACA GAATGACGAA AAGGGTGGCT ACAGTGTCAA TGGAGGATCT
    GGGGAAAATA CTTATGGTCG GAAGTCGTTG GGGCAAGAGC TGAGGGTTAA CAATGTGACC
    AGCCCTGAGT TTACCAGTAT TCAGCATGGC AGTCGTGCGT TAGCCATCAA AGACATGAGG
    AAATCACAGG AGCGATCGAT GTCTTATTCT GATGAGTCTC GACTGTCGAA TCTTCTTCGG
    AGGATCACCC GGGAAGATGA CAGAGACCGA AGGCTGGCTA CTGTTAAGCA GTTGAAAGAA
    TTTATTCAGC AACCAGAAAA TAAGCTGGTG CTAGTTAAGC AGTTGGATAA TATCTTGGCT
    GCTGTACATG ATGTGCTTAA TGAAAGTAGC AAATTGCTTC AGGAGTTGAG ACAGGAGGGA
    GCTTGTTGTC TTGGCCTTCT TTGTGCATCT CTGAGCTATG AGGCTGAGAA GATCTTCAAA
    TGGATTTTTA GCAAATTTAG CTCATCTGCA AAAGATGAAG TTAAACTCCT CTACTTATGT
    GCCACCTACA AAGCACTAGA GACTGTAGGA GAAAAGAAAG CCTTTTCATC TGTAATGCAG
    CTTGTAATGA CCAGCCTGCA GTCCATTCTT GAAAATGTGG ATACACCAGA ATTACTTTGC
    AAGTGTGTTA AGTGCATTCT TTTGGTAGCT CGATGTTACC CTCATATTTT CAGCACAAAT
    TTTAGGGATA CAGTTGATAT ATTAGTTGGA TGGCATATAG ATCATACTCA GAAGCCTTCA
    CTTACACAAC AGGTGTCTGG GTGGTTGCAG AGTTTGGAGC CATTCTGGGT AGCTGACCTT
    GCATTTTCTA CCACTCTTCT TGGTCAGTTT TTAGAGGACA TGGAGGCATA TGCTGAGGAC
    CTCAGCCATG TAGCCTCTGG GGAGTCAGTG GATGAAGATG TTCCTCCTCC ATCAGTGTCC
    TTACCAAAGC TGGCAGCACT TCTCCGGGTA TTCAGTACTG TGGTGAGAAG CATTGGGGAA
    CGTTTCAGCC CAATTCGAGG TCCTCCAATT ACTGAGGCAT ATGTAACAGA TGTTCTGTAC
    AGAGTAATGA GATGTGTGAC GGCTGCAAAC CAAGTGTTTT TTTCTGAGGC TGTGTTGACA
    GCTGCTAATG AGTGTGTTGG TGTTTTGCTC GGCAGCTTGG ATCCTAGCAT GACTATACAT
    TGTGACATGG TTATTACATA TGGATTAGAT CAACTGGAGA ATTGCCAGAC TTGTGGTACA
    GATTATATCA TCTCAGTCTT GAATTTACTC ACATTGATTG TTGAACAGAT AAATACAAAA
    CTGCCATCAT CATTTGTAGA AAAACTGTTT ATACCATCAT CTAAGCTACT ATTTTTGCGT
    TATCATAAAG AAAAAGAGGT TGTTGCTGTA GCCCATGCTG TTTATCAAGC AGTGCTCAGT
    CTGAAGAATA TTCCTGTTTT AGAGACTGCC TATAAATTAA TTTTGGGAGA AATGACTTGT
    GCCCTAAACA ATCTGCTACA TAGTCTACAA CTTCCTGATG CCTGTTCTGA AATAAAACAT
    GAGGCTTTTA AGAATCATGT TTTCAATGTA GACAATGCAA AATTTGTAGT TATATTTGAC
    CTTAGTGCTC TGACTACAAT TGGAAATGCT AAAAACTCAC TAATTGGGAT GTGGGCCCTG
    TCTCCAACTG TGTTTGCACT TCTGAGTAAG AATCTGATGA TTGTGCATGG CGACCTGGCT
    GTTCATTTCC CTGCCATTCA GTATGCAGTG CTCTACACAT TGTATTCTCA TTGTACCAGG
    CATGACCACT TTATATCTAG TAGTCTTAGT TCATCATCCC CGTCCTTGTT TGATGGTGCC
    GTTATTAGTA CTGTAACTAC AGCTACAAAA AAACATTTCT CAATTATCTT AAATCTCCTG
    GGAATATTGC TTAAGAAAGA TAATCTTAAC CAGGACACAA GGAAACTGTT AATGACCTGG
    GCTTTGGAAG TGGCTGTTTT AATGAAGAAG TCTGAAACAT ATGCACCTTT ATTCTCTCTT
    CCATCTTTCC ATAAATTTTG CAAAGGCCTT TTAGCCAACA CTCTGGTCGA AGATGTGAAT
    ATCTGTCTGC AAGCATGCAG CAGTCTACAT GCACTGTCCT CTTCCTTACC AGATGATCTT
    TTACAGAGGT GTGTTGATGT TTGCCGTGTT CAGCTAGTCC ATAGTGGAAC TCGTATTCGA
    CAAGCATTTG GAAAGCTATT GAAGTCAATC CCTTTAGATG TTGTCTTGAG CAATAACAAT
    CACACAGAAA TTCAAGAAAT TTCTTTAGCA TTAAGAAGTC ACATGAGTAA AGCACCAAGT
    AACACATTCC ACCCACAAGA TTTCTCTGAT GTTATTAGTT TTATTTTGTA TGGAAACTCT
    CATAGAATAG GGAAGGACAA TTGGTTAGAA AGATTGTTCT ATAGCTGCCA GAGACTGGAT
    AAACGTGACC AATCAACGAT TCCCCGCAAT CTGCTAAAGA CAGATGCTGT CCTCTGGCAG
    TGGGCTATTT GGGAAGCAGC ACAGTTCACT GTCCTTTCTA AGCTGAGGAC CCCACTTGGC
    AGAGCTCAGG ATACCTTCCA AACAATTGAA GGTATCATTA GAAGTCTTGC AGCTCACACA
    TTAAACCCCG ATCAAGATGT TAGTCAGTGG ACAACTGCAG ACAATGACGA AGGCCACAGT
    AGCAACCAGC TTAGACTTGT GCTGCTTCTG CAGTATCTGG AAAACCTGGA GAAGTTAATG
    TATAATGCAT ATGAAGGATG TGCCAATGCA TTAACATCGC CTCCCAAGGT CATTAGGACT
    TTTTTCTATA CCAATCGCCA AACTTGTCAA GACTGGCTGA CTCGGATCCG ACTCTCCATC
    ATGAGGGTTG GATTGTTGGC AGGCCAGCCT GCTGTGACAG TGAGACACGG CTTCGATTTG
    CTTACAGAAA TGAAGACAAA TCCGTCTCAG GGAAATGAAT TGGAAGTAAC TATCATGATG
    GTGGTAGAAG CATTGTGTGA ACTTCATTGT CCTGAAGCTA TACAGGGAAT TGCTGTCTGG
    TCGTCTTCGG TTGTTGGAAA AAATCTTCTC TGGATTAATT CAGTGGCCCA ACAGGCTGAA
    GGGAGGTTTG AAAAGGCCTC TGTGGAGTAC CAGGAGCACC TGTGTGCCAT GACAGGTGTC
    GATTGCTGCA TCTCTAGCTT CGACAAATCC GTTCTCACAT TGGCTAATAC TGGGCGTAAT
    AGTGCCAGCC CCAAGCATTC TTTGAATGGT GAATCCAGAA AAACTGTGCT TTCCAAACCA
    ACTGATTCTT CTCCTGAGGT TATAAATTAT TTGGGAAATA AAGCATGTGA GTGCTACATT
    TCAATAGCTG ATTGGGCTGC TGTACAGGAG TGGCAGAATG CTGTCCATGA CTTGAAAAAG
    AGTACCAGTA GTACTTCTCT CAACCTAAAG GCTGATTTCA ACTACATAAA ATCACTAAGC
    AGTTTTGAAT CTGGAGAATT TATTGAATGC ACTGAACAAT TAGAATTGTT ACCAGGAGAA
    AATATCAGTC TACTTGCTGG AGGATCAAAA GAAAAAATAG ATATGAAAAA ACTGCTTCCT
    AACATGTTAA GTCCAGATCC AAGAGAACTT CAGAAATCCA TTGAAGTTCA GTTGTTAAGA
    AGTTCTGTTT GTTTGGCAAC TGCTTTAAAC CACATAGAAC AAGATCAGAA GTGGCAGTCT
    ATTACCGAAA ATGTGGTGAA GTACCTGAAG CAAACCTCCC GCATAGCTGT TGGACCCCTG
    AGACTTTCTA CTCTAACAGT TTCCCAGTCT CTGCCAGTCC TAAGTACTTT ACAGCTGTAT
    TGCTCTTCTG CTTTGGAGAA CACAGTTTCC AACAGACTTG CCACAGAGGA CTGTCTTATT
    CCACTCTTTA GTGAAGCTTT GCGTTCATGT AAACAGCATG ATGTCAGGCC ATGGATGCAA
    GCATTAAGAT ACACGATGTA CCAGAGTCAG TTGTTGGAGA AAATAAAAGA ACAAACAGTT
    CCAATTAGAA GCCATCTCAT GGAATTAGGT CTAACAGCAG CAAAATTTGC TAGAAAACGG
    GGGAATGTGT CACTTGCAAC AAGATTGCTG GCACAGTGTA GCGAGGTTCA GCTGGGAAAG
    ACTACCACTG CACAGGATTT AGTCCAGCAC TTTAAAAAAC TATCAACTCA AGGTCAAGTG
    GATGAAAAAT GGGGGCCTGA ACTTGACATT GAAAAAACGA AACTGCTATA TACAGCAGGC
    CAGTCAACAC ATGCAATGGA GATGTTGAGT TCCTGTGCCA TCTCTTTCTG CAAGTCTGCC
    AAAGCTGAGC ATGCTGTTGC CAAGTCAATT TTGACATTGG CTAAATGGAT CCAGGCAGAA
    TGGAAAGAAA TCTCAGGACA GCTGAAACAG GTTTACAGAG CTCAGCAGCA GCAGAACTTC
    ACGGGTCTTT CTACTTTGTC TAAAAACATA CTCACTCTAA TTGAACTACC ATCTGTTAAT
    ACGATGGAGG AAGAGTATCC TCGGATAGAG AGTGAATCTA CAGTACATAT TGGAGTTGGG
    GAACCTGACT TCATCTTGGG ACAGCTGTAT CATCTGTCTT CAGTACAGGC ACCTGAAGTA
    GCCAAATCTT GGGCCGCATT GGCTAGTTGG GCTTACAGGT GGGGCAGAAA GGTGGTTGAC
    AATGCCAGTC AGGGAGAAGG TGTTCGTCTG TTGCCTAGAG AAAAATCTGA AGTTCAGAAT
    CTGCTCCCAG ACACCATAAC CGAAGAAGAA AAAGAGAGAA TATATGGTAT TCTTGGTCAG
    GCTGTGTGTC GCCCAGCGGG GATTCAGGAT GAAGATATAA CGCTTCAGAT CACAGAAAGT
    GAAGATAATG AGGAAGATGA CATGGTTGAT GTTATCTGGC GTCAGTTAAT ATCAAGTTGC
    CCATGGCTTT CAGAACTTGA TGAAAGTGCA ACAGAAGGAG TCATTAAAGT GTGGAGGAAA
    GTTGTGGACA GAATCTTCAG CCTGTATAAA CTGTCCTGCA GCGCATATTT TACTTTCCTT
    AAACTCAATG CTGGGCAGAT TCCTTTAGAT GAAGATGACC CTCGGCTCCA TTTAAGCCAC
    AGAGTGGAGC AGAGCACGGA TGACATGATT GTGATGGCCA CGCTGCGGTT GCTCAGGTTG
    CTCGTGAAGC ACGCTGGGGA GCTTCGGCAG TATCTGGAAC ATGGCTTGGA AACAACACCT
    ACTGCGCCTT GGAGAGGAAT TATTCCGCAG CTTTTCTCAC GCTTAAACCA CCCTGAAGTG
    TATGTGCGCC AGAGTATTTG TAACCTCCTC TGCCGCGTGG CTCAGGATTC CCCGCATCTC
    ATACTGTATC CTGCAATAGT GGGCACCATA TCGCTTAGTA GTGAGTCACA GGCTTCAGGA
    AATAAATTTT CCTCTGCAAT TCCAACTTTA CTTGGCAATA TTCAAGGAGA AGAACTACTG
    GTTTCTGAAT GTGAGGGAGG AAGTCCTCCT GCTTCTCAAG ATAGCAATAA GGATGAACCT
    AAAAGTGGAC TGAATGAAGA CCAAGCCATG ATGCAGGACT GCTACAGCAA AATTGTAGAT
    AAACTGTCCT CGGCAAACCC AACTATGGTG TTACAGGTTC AGATGCTTGT GGCTGAGCTG
    CGCAGGGTCA CTGTTCTCTG GGATGAGCTC TGGTTGGGAG TTCTGTTACA GCAGCACATG
    TATGTCCTGA GACGAATTCA GCAGTTGGAA GACGAGGTGA AGAGAGTCCA GAACAACAAC
    ACCTTACGCA AAGAAGAGAA AATCGCAATC ATGCGGGAGA AGCACACAGC TTTGATGAAG
    CCCATCGTAT TTGCTTTGGA GCACGTTAGG AGCATCACAG CGGCCCCTGC AGAAACACCT
    CATGAAAAGT GGTTTCAGGA TAACTATGGT GATGCCATTG AAAATGCTCT AGAAAAGCTG
    AAAACACCGT CAAACCCTGC AAAGCCTGGA AGCAGCTGGA TTCCATTTAA AGAGATAATG
    CTGAGTTTGC AACAGAGAGC ACAGAAACGT GCAAGTTACA TCTTGCGTCT TGAAGAAATC
    AGCCCATGGC TGGCTGCCAT GACTAACACT GAAATTGCTC TTCCCGGAGA AGTCTCAGCC
    AGAGATACCG TCACAATCCA CAGTGTTGGT GGAACCATCA CAATCTTACC TACTAAAACC
    AAGCCAAAGA AACTTCTCTT TCTAGGATCA GATGGGAAGA GCTATCCTTA TCTTTTCAAA
    GGATTGGAGG ATTTACATCT GGATGAGAGA ATAATGCAAT TTCTGTCTAT TGTAAATACC
    ATGTTTGCAA CAATCAATCG CCAGGAAACA CCTCGTTTCC ATGCTCGCCA CTATTCTGTA
    ACACCACTGG GAACTCGCTC AGGCCTAATC CAGTGGGTGG ATGGAGCCAC GCCCTTATTT
    GGTCTTTACA AACGATGGCA ACAACGGGAA GCCGCCTTGC AAGCACAAAA GGCCCAAGAT
    TCCTACCAAA CTCCTCAGAA TCCTGGAATC GTACCCCGTC CTAGTGAACT TTATTATAGT
    AAAATTGGTC CTGCTTTGAA AGCAGTTGGG CTTAGTCTGG ATGTGTCCCG CCGGGATTGG
    CCTCTTCATG TGATGAAGGC AGTGTTAGAA GAGTTAATGG AGGCCACGCC CCCAAATCTC
    CTTGCTAAAG AGCTTTGGTC ATCATGTACC ACCCCTGATG AGTGGTGGAG AGTCACACAG
    TCTTATGCCA GATCTACTGC AGTCATGTCT ATGGTCGGAT ACATAATTGG CCTTGGAGAT
    AGACATCTGG ATAATGTTCT CATAGATATG ACGACTGGAG AGGTTGTTCA CATAGATTAC
    AATGTTTGCT TTGAAAAAGG TAAAAGCCTT AGAGTTCCTG AGAAAGTACC TTTTCGAATG
    ACACAGAACA TTGAAACAGC ACTGGGTGTA ACTGGAGTAG AAGGTGTTTT TAGGCTTTCA
    TGTGAGCAGG TTCTACACAT TATGCGGCGT GGCAGAGAGA CTCTGCTGAC GCTGCTGGAG
    GCCTTTGTAT ATGACCCCCT GGTGGACTGG ACAGCTGGGG GCGAGGCTGG GTTTGCTGGT
    GCGGTCTATG GTGGAGGGGG CCAGCAGGCT GAGAGCAAGC AGAGCAAGAG AGAGATGGAA
    CGAGAGATTA CTCGCAGCCT TTTTTCTTCG AGAGTAGCTG AAATTAAGGT GAACTGGTTT
    AAAAACAGAG ATGAGATGCT GGTTGTACTT CCCAAGCTGG ACAGCAGCTT AGATGAATAC
    CTGAGCTTGC AAGAGCAACT AACAGATGTG GAGAAACTGC AGGGCAAACT GCTGGAAGAA
    ATTGAGTTTC TAGAAGGAGC TGAAGGAGTG GATCATCCTT CTCATACTCT GCAACACAGG
    TATTCTGAAC ACACTCAACT ACAGACTCAG CAGAGAGCTG TTCAGGAAGC AATCCAGGTG
    AAGCTGAATG AATTTGAACA GTGGATAACA CACTATCAGG CTGCGTTCAA TAATTTGGAA
    GCTACACAGC TGGCCAGTTT GCTTCAGGAG ATAAGCACAC AGATGGACCT TGGTCCTCCC
    AGCTACGTGC CGGCAACAGC CTTTCTGCAG AATGCTGGCC AGGCCCACTT GATTAGCCAG
    TGTGAGCAGC TAGAAGGGGA GGTTGGTGCC CTCCTGCAGC AGAGGCGCTC GGTGCTGCGA
    GGCTGTCTGG AGCAGCTGCA TCACTACGCA ACTGTTGCTC TGCAGTATCC AAAGGCCATA
    TTTCAGAAAC ATCGAATTGA GCAGTGGAAG ACCTGGATGG AAGAGCTCAT CTGTAATACC
    ACAGTAGAAC GCTGTCAGGA ACTTTACAGG AAATATGAAA TGCAGTATGC GCCCCAGCCA
    CCCCCAACAG TGTGTCAGTT CATCACGGCC ACTGAGATGA CCCTGCAGCG GTACGCAGCC
    GACATCAACA GCCGACTTAT CAGACAGGTG GAACGCTTGA AACAGGAAGC TGTCACTGTA
    CCGGTTTGTG AAGATCAGTT GAAAGAAATT GAACGTTGTA TCAAAGTTTT CCTTCACGAG
    AATGGAGAGG AAGGATCTTT GAGTCTAGCA AGTGTTATTA TTTCTGCCCT TTGTACTCTC
    ACAAGGCGTA ACCTAATGAT GGAAGGTGCA GCGTCGAGTG CTGGAGAGCA GCTGGTTGAT
    CTGACTTCTC GGGATGGAGC CTGGTTCTTG GAGGAACTCT GCAGTATGAG CGGAAATGTC
    ACATGTCTGG TTCAGTTACT GAAGCAGTGT CATTTAGTGC CACAAGACTT AGACATTCCT
    AACCCCATGG AAGCATCTGA GGCCGTTCAT TTGGCTAATG GTGTATACAC CTCACTTCAG
    GAATTAAATT CAAATTTCCG GCAAATCATA TTTCCAGAAG CACTGAGATG TTTAATGAAA
    GGAGAATACA CGTTAGAAAA TATGCTTCAT GAGCTGGACA GTCTTATTGA GCAGACAACT
    GATGGTGTTC CTCTGCAGAC TCTAGTTGAA TCTCTTCAAG CCTATTTAAG AAATGCAGCT
    ATGGGACTTG AGGAAGAAAC ACATGCTCAT TACATCGATG TTGCCAGAAT GCTTCATGCT
    CAGTATGGTG AATTAATCCA ACCAAGAAAT GGTTCAGTTG ATGAAACACC AAAAATGTCA
    GCTGGCCAGA TGCTTTTGGT AGCATTTGAT GGCATGTTTG CCCAAGTTGA GACTGCTTTT
    GGCTTATTGG TTGAAAAGCT AAATAAGATG GAAATTCCTA TAGCTTGGCG AAAGATTGAC
    ATAATACGGG AAGCCAGGAG CACTCAGGTC AATTTTTTTG ATGATGATAA TCACCGACAG
    GTGCTGGAAG AGATCTTCTT TCTAAAACGA CTACAGACAA TTAAGGAGTT CTTTCGGCTC
    TGTGGTACCT TTTCTAAAAC TTTGTCAGGA TCAAGTTCAC TTGAAGATCA GAATACTGTA
    AATGGGCCTG TACAGATTGT CAATGTGAAA ACCCTTTTTA GAAACTCTTG TTTCAGTGAA
    GACCAGATGG CCAAACCAAT AAAGGCATTC ACAGCTGACT TTGTGCGGCA GCTCCTGATT
    GGACTCCCGA ACCAAGCCCT GGGCCTCACA CTGTGCAGCT TCATCAGTGC TCTAGGGGTA
    GACATCATTG CGCAGGTGGA GGCGAAGGAT TTTGGTGCTG AAAGCAAAGT TTCTGTTGAC
    GATCTGTGTA AGAAGGCGGT GGAGCACAAC ATCCAGATCG GGAAGTTCTC TCAGCTGGTC
    ATGAACAGGG CGACTGTGCT GGCGAGCTCC TACGACACTG CCTGGAAGAA GCACGACTTG
    GTGCGCAGGC TGGAGACCAG CATCGCCTCC TGCAAGACAA GCCTGCAGCG TGTCCAGCTG
    CACATCGCCA TGTTTCAGTG GCAGCATGAA GATCTCCTTA TCAATAGACC ACAAGCCATG
    TCTGTCACAC CTCCCCCTCG TTCTGCCATA TTAACCAGCA TGAAAAAGAA ACTCCATACT
    CTGAGCCAGA TTGAAACTTC AATTGCAACA GTTCAGGAGA AACTGGCAGC ACTTGAAGCA
    AGTATTGAAC AGCGACTCAA ATGGGCAGGT GGTGCCAACC CTGCACTGGC ACCTGTACTA
    CAGGATTTTG AAGCAACAAT AGCTGAAAGA AGAAATCTTG TCCTTAAGGA GAGCCAAAGA
    GCAAGTCAGG TCACTTTCCT CTGCAGCAAT ATCATTCATT TTGAAAGTTT ACGAACTAGA
    ACTGCAGAAG CCTTAAACCT GGATGCTGCA TTATTTGAAC TAATCAAACG GTGTCAGCAA
    ATGTGTTCAT TTGCATCGCA GTTTAACAGT TCAGTCTCTG AGTTAGAGCT TCGTTTATTA
    CAGAGGGTGG ACACGACTCT TGAACATCCT ATTGGCAGCT CTGAGTGGCT TTTGTCAGCA
    CACAAACAAC TGACCCAGGA TATGTCTACT CAGAGAGCAA TTCAGACAGA GAAAGAGCAA
    CAAATAGAAA CAGTCTGTGA AACAATTCAG AATCTGGTTG ATAATATAAA GACTGTGCTC
    ACTGGCCATA ACCGGCAGCT TGGAGATGTC AAACATCTCC TGAAAGCAAT GGCCAAGGAT
    GAAGAAGCTG CTCTGGCAGA TGGTGAAGAT GTTCCCTATG AGAACAGTGT TAGGCAGTTC
    TTGGGTGAAT ATAAATCATG GCAAGACAAC ATTCAGACAG TGCTGTTTAC ATTAGTCCAA
    GCCATGGGTC AGGTTCGAAG TCAAGAACAT GTTGAAATGC TCCAGGAAAT AACTCCCACC
    TTGAAAGAAC TGAAAACGCA AAGTCAGAGT GTGTATAATA ATTTAGTGAG TTTTGCATCA
    CCATTAGTGA CCGATGCAAC AAATGAATGT TCGAGTCCAA CATCGTCTGC TACTTATCAA
    CCATCCTTTG CTGCAGCAGT CCGAAGCAAC ACTGGCCAGA AGACTCAGCC TGATGTCATG
    TCACAGAACG CTAGAAAGCT GATTCAGAAA AACCTAGCTA CATCAGCAGA TACCCCACCG
    AGCACCGTTC CGGGAACTGG CAAGAGTGTT GCTTGTAGTC CTAAAAAGGC CATCAGAGAC
    CCTAAGACTG GAAAAGCTGT GCAGGAGAGA AACTCGTATG CGGTGAGTGT GTGGAAGAGA
    GTGAAAGCCA AGCTAGAGGG CCGAGATGTT GATCCGAATA GGAGGATGTC AGTTGCTGAA
    CAGGTTGACT ATGTCATTAA GGAAGCAACT AATCTAGATA ACTTGGCTCA GCTGTATGAA
    GGTTGGACAG CCTGGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32979
    Clone ID Related Accession (Same CDS sequence) XM_005224745.3 , XM_005224745.4
    Accession Version XM_005224745.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase SMG1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
    GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT
    CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG
    TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC
    TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG
    TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT
    TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC
    GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA
    CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA
    ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG
    AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT
    AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG
    TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC
    CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT
    GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG
    ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT
    GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC
    GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG
    GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG
    GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC
    CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC
    GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA
    ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA
    ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC
    CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC
    ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT
    ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG
    CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT
    GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT
    GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA
    AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG
    TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA
    AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT
    CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG
    AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT
    GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT
    TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG
    GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG
    CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA
    GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT
    GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT
    CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT
    CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA
    TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC
    TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG
    ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG
    CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA
    CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT
    ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG
    GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA
    AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT
    GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT
    ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG
    ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA
    ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA
    GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT
    TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC
    CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG
    TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG
    GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC
    CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT
    TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA
    CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA
    GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT
    AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC
    AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT
    GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA
    CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC
    CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA
    GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT
    GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT
    CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT
    AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT
    CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA
    GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA
    CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT
    TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT
    ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC
    CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC
    ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA
    AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA
    CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC
    CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG
    CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG
    AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT
    GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT
    CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA
    CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT
    TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG
    ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT
    GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG
    AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG
    GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC
    TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA
    CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT
    CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA
    GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC
    AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC
    CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC
    TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC
    TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT
    CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC
    ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT
    GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG
    CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA
    GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT
    TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC
    CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG
    CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG
    AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC
    TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC
    ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA
    TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT
    GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA
    AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA
    ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA
    CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT
    GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG
    CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT
    AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG
    TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT
    CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT
    TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG
    CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT
    GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA
    GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC
    TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG
    AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG
    CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA
    CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG
    AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA
    GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG
    GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG
    GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT
    TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA
    CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG
    CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG
    CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA
    GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA
    AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA
    ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT
    GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT
    ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA
    ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG
    AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT
    GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG
    CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA
    GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG
    GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT
    GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG
    TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT
    CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224802.1
    CDS25..9117
    Translation

    Target ORF information:

    RefSeq Version XM_005224745.3
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG1 phosphatidylinositol 3-kinase-related kinase (SMG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224745.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
    GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT
    CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG
    TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC
    TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG
    TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT
    TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC
    GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA
    CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA
    ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG
    AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT
    AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG
    TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC
    CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT
    GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG
    ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT
    GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC
    GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG
    GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG
    GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC
    CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC
    GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA
    ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA
    ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC
    CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC
    ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT
    ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG
    CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT
    GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT
    GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA
    AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG
    TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA
    AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT
    CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG
    AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT
    GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT
    TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG
    GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG
    CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA
    GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT
    GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT
    CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT
    CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA
    TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC
    TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG
    ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG
    CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA
    CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT
    ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG
    GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA
    AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT
    GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT
    ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG
    ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA
    ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA
    GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT
    TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC
    CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG
    TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG
    GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC
    CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT
    TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA
    CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA
    GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT
    AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC
    AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT
    GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA
    CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC
    CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA
    GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT
    GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT
    CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT
    AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT
    CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA
    GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA
    CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT
    TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT
    ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC
    CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC
    ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA
    AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA
    CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC
    CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG
    CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG
    AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT
    GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT
    CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA
    CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT
    TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG
    ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT
    GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG
    AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG
    GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC
    TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA
    CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT
    CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA
    GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC
    AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC
    CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC
    TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC
    TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT
    CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC
    ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT
    GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG
    CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA
    GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT
    TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC
    CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG
    CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG
    AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC
    TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC
    ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA
    TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT
    GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA
    AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA
    ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA
    CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT
    GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG
    CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT
    AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG
    TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT
    CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT
    TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG
    CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT
    GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA
    GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC
    TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG
    AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG
    CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA
    CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG
    AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA
    GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG
    GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG
    GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT
    TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA
    CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG
    CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG
    CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA
    GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA
    AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA
    ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT
    GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT
    ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA
    ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG
    AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT
    GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG
    CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA
    GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG
    GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT
    GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG
    TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT
    CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32979
    Clone ID Related Accession (Same CDS sequence) XM_005224745.3 , XM_005224745.4
    Accession Version XM_005224745.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase SMG1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224745.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
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    2821
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    2941
    3001
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    3121
    3181
    3241
    3301
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    3421
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    4141
    4201
    4261
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    4381
    4441
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    4681
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    4981
    5041
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    5221
    5281
    5341
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    5521
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    5641
    5701
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    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
    GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT
    CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG
    TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC
    TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG
    TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT
    TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC
    GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA
    CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA
    ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG
    AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT
    AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG
    TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC
    CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT
    GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG
    ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT
    GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC
    GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG
    GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG
    GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC
    CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC
    GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA
    ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA
    ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC
    CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC
    ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT
    ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG
    CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT
    GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT
    GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA
    AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG
    TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA
    AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT
    CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG
    AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT
    GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT
    TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG
    GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG
    CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA
    GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT
    GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT
    CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT
    CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA
    TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC
    TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG
    ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG
    CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA
    CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT
    ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG
    GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA
    AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT
    GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT
    ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG
    ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA
    ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA
    GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT
    TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC
    CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG
    TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG
    GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC
    CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT
    TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA
    CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA
    GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT
    AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC
    AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT
    GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA
    CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC
    CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA
    GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT
    GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT
    CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT
    AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT
    CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA
    GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA
    CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT
    TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT
    ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC
    CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC
    ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA
    AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA
    CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC
    CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG
    CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG
    AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT
    GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT
    CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA
    CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT
    TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG
    ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT
    GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG
    AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG
    GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC
    TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA
    CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT
    CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA
    GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC
    AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC
    CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC
    TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC
    TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT
    CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC
    ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT
    GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG
    CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA
    GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT
    TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC
    CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG
    CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG
    AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC
    TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC
    ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA
    TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT
    GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA
    AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA
    ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA
    CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT
    GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG
    CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT
    AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG
    TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT
    CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT
    TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG
    CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT
    GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA
    GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC
    TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG
    AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG
    CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA
    CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG
    AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA
    GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG
    GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG
    GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT
    TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA
    CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG
    CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG
    CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA
    GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA
    AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA
    ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT
    GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT
    ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA
    ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG
    AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT
    GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG
    CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA
    GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG
    GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT
    GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG
    TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT
    CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224802.1
    CDS39..9131
    Translation

    Target ORF information:

    RefSeq Version XM_005224745.4
    Organism Bos taurus(cattle)
    Definition Bos taurus SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224745.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGTGGGCCC TGTCTCCAAC TGTGTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAT 
    GGCGACCTGG CTGTTCATTT CCCTGCCATT CAGTATGCAG TGCTCTACAC ATTGTATTCT
    CATTGTACCA GGCATGACCA CTTTATATCT AGTAGTCTTA GTTCATCATC CCCGTCCTTG
    TTTGATGGTG CCGTTATTAG TACTGTAACT ACAGCTACAA AAAAACATTT CTCAATTATC
    TTAAATCTCC TGGGAATATT GCTTAAGAAA GATAATCTTA ACCAGGACAC AAGGAAACTG
    TTAATGACCT GGGCTTTGGA AGTGGCTGTT TTAATGAAGA AGTCTGAAAC ATATGCACCT
    TTATTCTCTC TTCCATCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTGGTC
    GAAGATGTGA ATATCTGTCT GCAAGCATGC AGCAGTCTAC ATGCACTGTC CTCTTCCTTA
    CCAGATGATC TTTTACAGAG GTGTGTTGAT GTTTGCCGTG TTCAGCTAGT CCATAGTGGA
    ACTCGTATTC GACAAGCATT TGGAAAGCTA TTGAAGTCAA TCCCTTTAGA TGTTGTCTTG
    AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT
    AAAGCACCAA GTAACACATT CCACCCACAA GATTTCTCTG ATGTTATTAG TTTTATTTTG
    TATGGAAACT CTCATAGAAT AGGGAAGGAC AATTGGTTAG AAAGATTGTT CTATAGCTGC
    CAGAGACTGG ATAAACGTGA CCAATCAACG ATTCCCCGCA ATCTGCTAAA GACAGATGCT
    GTCCTCTGGC AGTGGGCTAT TTGGGAAGCA GCACAGTTCA CTGTCCTTTC TAAGCTGAGG
    ACCCCACTTG GCAGAGCTCA GGATACCTTC CAAACAATTG AAGGTATCAT TAGAAGTCTT
    GCAGCTCACA CATTAAACCC CGATCAAGAT GTTAGTCAGT GGACAACTGC AGACAATGAC
    GAAGGCCACA GTAGCAACCA GCTTAGACTT GTGCTGCTTC TGCAGTATCT GGAAAACCTG
    GAGAAGTTAA TGTATAATGC ATATGAAGGA TGTGCCAATG CATTAACATC GCCTCCCAAG
    GTCATTAGGA CTTTTTTCTA TACCAATCGC CAAACTTGTC AAGACTGGCT GACTCGGATC
    CGACTCTCCA TCATGAGGGT TGGATTGTTG GCAGGCCAGC CTGCTGTGAC AGTGAGACAC
    GGCTTCGATT TGCTTACAGA AATGAAGACA AATCCGTCTC AGGGAAATGA ATTGGAAGTA
    ACTATCATGA TGGTGGTAGA AGCATTGTGT GAACTTCATT GTCCTGAAGC TATACAGGGA
    ATTGCTGTCT GGTCGTCTTC GGTTGTTGGA AAAAATCTTC TCTGGATTAA TTCAGTGGCC
    CAACAGGCTG AAGGGAGGTT TGAAAAGGCC TCTGTGGAGT ACCAGGAGCA CCTGTGTGCC
    ATGACAGGTG TCGATTGCTG CATCTCTAGC TTCGACAAAT CCGTTCTCAC ATTGGCTAAT
    ACTGGGCGTA ATAGTGCCAG CCCCAAGCAT TCTTTGAATG GTGAATCCAG AAAAACTGTG
    CTTTCCAAAC CAACTGATTC TTCTCCTGAG GTTATAAATT ATTTGGGAAA TAAAGCATGT
    GAGTGCTACA TTTCAATAGC TGATTGGGCT GCTGTACAGG AGTGGCAGAA TGCTGTCCAT
    GACTTGAAAA AGAGTACCAG TAGTACTTCT CTCAACCTAA AGGCTGATTT CAACTACATA
    AAATCACTAA GCAGTTTTGA ATCTGGAGAA TTTATTGAAT GCACTGAACA ATTAGAATTG
    TTACCAGGAG AAAATATCAG TCTACTTGCT GGAGGATCAA AAGAAAAAAT AGATATGAAA
    AAACTGCTTC CTAACATGTT AAGTCCAGAT CCAAGAGAAC TTCAGAAATC CATTGAAGTT
    CAGTTGTTAA GAAGTTCTGT TTGTTTGGCA ACTGCTTTAA ACCACATAGA ACAAGATCAG
    AAGTGGCAGT CTATTACCGA AAATGTGGTG AAGTACCTGA AGCAAACCTC CCGCATAGCT
    GTTGGACCCC TGAGACTTTC TACTCTAACA GTTTCCCAGT CTCTGCCAGT CCTAAGTACT
    TTACAGCTGT ATTGCTCTTC TGCTTTGGAG AACACAGTTT CCAACAGACT TGCCACAGAG
    GACTGTCTTA TTCCACTCTT TAGTGAAGCT TTGCGTTCAT GTAAACAGCA TGATGTCAGG
    CCATGGATGC AAGCATTAAG ATACACGATG TACCAGAGTC AGTTGTTGGA GAAAATAAAA
    GAACAAACAG TTCCAATTAG AAGCCATCTC ATGGAATTAG GTCTAACAGC AGCAAAATTT
    GCTAGAAAAC GGGGGAATGT GTCACTTGCA ACAAGATTGC TGGCACAGTG TAGCGAGGTT
    CAGCTGGGAA AGACTACCAC TGCACAGGAT TTAGTCCAGC ACTTTAAAAA ACTATCAACT
    CAAGGTCAAG TGGATGAAAA ATGGGGGCCT GAACTTGACA TTGAAAAAAC GAAACTGCTA
    TATACAGCAG GCCAGTCAAC ACATGCAATG GAGATGTTGA GTTCCTGTGC CATCTCTTTC
    TGCAAGTCTG CCAAAGCTGA GCATGCTGTT GCCAAGTCAA TTTTGACATT GGCTAAATGG
    ATCCAGGCAG AATGGAAAGA AATCTCAGGA CAGCTGAAAC AGGTTTACAG AGCTCAGCAG
    CAGCAGAACT TCACGGGTCT TTCTACTTTG TCTAAAAACA TACTCACTCT AATTGAACTA
    CCATCTGTTA ATACGATGGA GGAAGAGTAT CCTCGGATAG AGAGTGAATC TACAGTACAT
    ATTGGAGTTG GGGAACCTGA CTTCATCTTG GGACAGCTGT ATCATCTGTC TTCAGTACAG
    GCACCTGAAG TAGCCAAATC TTGGGCCGCA TTGGCTAGTT GGGCTTACAG GTGGGGCAGA
    AAGGTGGTTG ACAATGCCAG TCAGGGAGAA GGTGTTCGTC TGTTGCCTAG AGAAAAATCT
    GAAGTTCAGA ATCTGCTCCC AGACACCATA ACCGAAGAAG AAAAAGAGAG AATATATGGT
    ATTCTTGGTC AGGCTGTGTG TCGCCCAGCG GGGATTCAGG ATGAAGATAT AACGCTTCAG
    ATCACAGAAA GTGAAGATAA TGAGGAAGAT GACATGGTTG ATGTTATCTG GCGTCAGTTA
    ATATCAAGTT GCCCATGGCT TTCAGAACTT GATGAAAGTG CAACAGAAGG AGTCATTAAA
    GTGTGGAGGA AAGTTGTGGA CAGAATCTTC AGCCTGTATA AACTGTCCTG CAGCGCATAT
    TTTACTTTCC TTAAACTCAA TGCTGGGCAG ATTCCTTTAG ATGAAGATGA CCCTCGGCTC
    CATTTAAGCC ACAGAGTGGA GCAGAGCACG GATGACATGA TTGTGATGGC CACGCTGCGG
    TTGCTCAGGT TGCTCGTGAA GCACGCTGGG GAGCTTCGGC AGTATCTGGA ACATGGCTTG
    GAAACAACAC CTACTGCGCC TTGGAGAGGA ATTATTCCGC AGCTTTTCTC ACGCTTAAAC
    CACCCTGAAG TGTATGTGCG CCAGAGTATT TGTAACCTCC TCTGCCGCGT GGCTCAGGAT
    TCCCCGCATC TCATACTGTA TCCTGCAATA GTGGGCACCA TATCGCTTAG TAGTGAGTCA
    CAGGCTTCAG GAAATAAATT TTCCTCTGCA ATTCCAACTT TACTTGGCAA TATTCAAGGA
    GAAGAACTAC TGGTTTCTGA ATGTGAGGGA GGAAGTCCTC CTGCTTCTCA AGATAGCAAT
    AAGGATGAAC CTAAAAGTGG ACTGAATGAA GACCAAGCCA TGATGCAGGA CTGCTACAGC
    AAAATTGTAG ATAAACTGTC CTCGGCAAAC CCAACTATGG TGTTACAGGT TCAGATGCTT
    GTGGCTGAGC TGCGCAGGGT CACTGTTCTC TGGGATGAGC TCTGGTTGGG AGTTCTGTTA
    CAGCAGCACA TGTATGTCCT GAGACGAATT CAGCAGTTGG AAGACGAGGT GAAGAGAGTC
    CAGAACAACA ACACCTTACG CAAAGAAGAG AAAATCGCAA TCATGCGGGA GAAGCACACA
    GCTTTGATGA AGCCCATCGT ATTTGCTTTG GAGCACGTTA GGAGCATCAC AGCGGCCCCT
    GCAGAAACAC CTCATGAAAA GTGGTTTCAG GATAACTATG GTGATGCCAT TGAAAATGCT
    CTAGAAAAGC TGAAAACACC GTCAAACCCT GCAAAGCCTG GAAGCAGCTG GATTCCATTT
    AAAGAGATAA TGCTGAGTTT GCAACAGAGA GCACAGAAAC GTGCAAGTTA CATCTTGCGT
    CTTGAAGAAA TCAGCCCATG GCTGGCTGCC ATGACTAACA CTGAAATTGC TCTTCCCGGA
    GAAGTCTCAG CCAGAGATAC CGTCACAATC CACAGTGTTG GTGGAACCAT CACAATCTTA
    CCTACTAAAA CCAAGCCAAA GAAACTTCTC TTTCTAGGAT CAGATGGGAA GAGCTATCCT
    TATCTTTTCA AAGGATTGGA GGATTTACAT CTGGATGAGA GAATAATGCA ATTTCTGTCT
    ATTGTAAATA CCATGTTTGC AACAATCAAT CGCCAGGAAA CACCTCGTTT CCATGCTCGC
    CACTATTCTG TAACACCACT GGGAACTCGC TCAGGCCTAA TCCAGTGGGT GGATGGAGCC
    ACGCCCTTAT TTGGTCTTTA CAAACGATGG CAACAACGGG AAGCCGCCTT GCAAGCACAA
    AAGGCCCAAG ATTCCTACCA AACTCCTCAG AATCCTGGAA TCGTACCCCG TCCTAGTGAA
    CTTTATTATA GTAAAATTGG TCCTGCTTTG AAAGCAGTTG GGCTTAGTCT GGATGTGTCC
    CGCCGGGATT GGCCTCTTCA TGTGATGAAG GCAGTGTTAG AAGAGTTAAT GGAGGCCACG
    CCCCCAAATC TCCTTGCTAA AGAGCTTTGG TCATCATGTA CCACCCCTGA TGAGTGGTGG
    AGAGTCACAC AGTCTTATGC CAGATCTACT GCAGTCATGT CTATGGTCGG ATACATAATT
    GGCCTTGGAG ATAGACATCT GGATAATGTT CTCATAGATA TGACGACTGG AGAGGTTGTT
    CACATAGATT ACAATGTTTG CTTTGAAAAA GGTAAAAGCC TTAGAGTTCC TGAGAAAGTA
    CCTTTTCGAA TGACACAGAA CATTGAAACA GCACTGGGTG TAACTGGAGT AGAAGGTGTT
    TTTAGGCTTT CATGTGAGCA GGTTCTACAC ATTATGCGGC GTGGCAGAGA GACTCTGCTG
    ACGCTGCTGG AGGCCTTTGT ATATGACCCC CTGGTGGACT GGACAGCTGG GGGCGAGGCT
    GGGTTTGCTG GTGCGGTCTA TGGTGGAGGG GGCCAGCAGG CTGAGAGCAA GCAGAGCAAG
    AGAGAGATGG AACGAGAGAT TACTCGCAGC CTTTTTTCTT CGAGAGTAGC TGAAATTAAG
    GTGAACTGGT TTAAAAACAG AGATGAGATG CTGGTTGTAC TTCCCAAGCT GGACAGCAGC
    TTAGATGAAT ACCTGAGCTT GCAAGAGCAA CTAACAGATG TGGAGAAACT GCAGGGCAAA
    CTGCTGGAAG AAATTGAGTT TCTAGAAGGA GCTGAAGGAG TGGATCATCC TTCTCATACT
    CTGCAACACA GGTATTCTGA ACACACTCAA CTACAGACTC AGCAGAGAGC TGTTCAGGAA
    GCAATCCAGG TGAAGCTGAA TGAATTTGAA CAGTGGATAA CACACTATCA GGCTGCGTTC
    AATAATTTGG AAGCTACACA GCTGGCCAGT TTGCTTCAGG AGATAAGCAC ACAGATGGAC
    CTTGGTCCTC CCAGCTACGT GCCGGCAACA GCCTTTCTGC AGAATGCTGG CCAGGCCCAC
    TTGATTAGCC AGTGTGAGCA GCTAGAAGGG GAGGTTGGTG CCCTCCTGCA GCAGAGGCGC
    TCGGTGCTGC GAGGCTGTCT GGAGCAGCTG CATCACTACG CAACTGTTGC TCTGCAGTAT
    CCAAAGGCCA TATTTCAGAA ACATCGAATT GAGCAGTGGA AGACCTGGAT GGAAGAGCTC
    ATCTGTAATA CCACAGTAGA ACGCTGTCAG GAACTTTACA GGAAATATGA AATGCAGTAT
    GCGCCCCAGC CACCCCCAAC AGTGTGTCAG TTCATCACGG CCACTGAGAT GACCCTGCAG
    CGGTACGCAG CCGACATCAA CAGCCGACTT ATCAGACAGG TGGAACGCTT GAAACAGGAA
    GCTGTCACTG TACCGGTTTG TGAAGATCAG TTGAAAGAAA TTGAACGTTG TATCAAAGTT
    TTCCTTCACG AGAATGGAGA GGAAGGATCT TTGAGTCTAG CAAGTGTTAT TATTTCTGCC
    CTTTGTACTC TCACAAGGCG TAACCTAATG ATGGAAGGTG CAGCGTCGAG TGCTGGAGAG
    CAGCTGGTTG ATCTGACTTC TCGGGATGGA GCCTGGTTCT TGGAGGAACT CTGCAGTATG
    AGCGGAAATG TCACATGTCT GGTTCAGTTA CTGAAGCAGT GTCATTTAGT GCCACAAGAC
    TTAGACATTC CTAACCCCAT GGAAGCATCT GAGGCCGTTC ATTTGGCTAA TGGTGTATAC
    ACCTCACTTC AGGAATTAAA TTCAAATTTC CGGCAAATCA TATTTCCAGA AGCACTGAGA
    TGTTTAATGA AAGGAGAATA CACGTTAGAA AATATGCTTC ATGAGCTGGA CAGTCTTATT
    GAGCAGACAA CTGATGGTGT TCCTCTGCAG ACTCTAGTTG AATCTCTTCA AGCCTATTTA
    AGAAATGCAG CTATGGGACT TGAGGAAGAA ACACATGCTC ATTACATCGA TGTTGCCAGA
    ATGCTTCATG CTCAGTATGG TGAATTAATC CAACCAAGAA ATGGTTCAGT TGATGAAACA
    CCAAAAATGT CAGCTGGCCA GATGCTTTTG GTAGCATTTG ATGGCATGTT TGCCCAAGTT
    GAGACTGCTT TTGGCTTATT GGTTGAAAAG CTAAATAAGA TGGAAATTCC TATAGCTTGG
    CGAAAGATTG ACATAATACG GGAAGCCAGG AGCACTCAGG TCAATTTTTT TGATGATGAT
    AATCACCGAC AGGTGCTGGA AGAGATCTTC TTTCTAAAAC GACTACAGAC AATTAAGGAG
    TTCTTTCGGC TCTGTGGTAC CTTTTCTAAA ACTTTGTCAG GATCAAGTTC ACTTGAAGAT
    CAGAATACTG TAAATGGGCC TGTACAGATT GTCAATGTGA AAACCCTTTT TAGAAACTCT
    TGTTTCAGTG AAGACCAGAT GGCCAAACCA ATAAAGGCAT TCACAGCTGA CTTTGTGCGG
    CAGCTCCTGA TTGGACTCCC GAACCAAGCC CTGGGCCTCA CACTGTGCAG CTTCATCAGT
    GCTCTAGGGG TAGACATCAT TGCGCAGGTG GAGGCGAAGG ATTTTGGTGC TGAAAGCAAA
    GTTTCTGTTG ACGATCTGTG TAAGAAGGCG GTGGAGCACA ACATCCAGAT CGGGAAGTTC
    TCTCAGCTGG TCATGAACAG GGCGACTGTG CTGGCGAGCT CCTACGACAC TGCCTGGAAG
    AAGCACGACT TGGTGCGCAG GCTGGAGACC AGCATCGCCT CCTGCAAGAC AAGCCTGCAG
    CGTGTCCAGC TGCACATCGC CATGTTTCAG TGGCAGCATG AAGATCTCCT TATCAATAGA
    CCACAAGCCA TGTCTGTCAC ACCTCCCCCT CGTTCTGCCA TATTAACCAG CATGAAAAAG
    AAACTCCATA CTCTGAGCCA GATTGAAACT TCAATTGCAA CAGTTCAGGA GAAACTGGCA
    GCACTTGAAG CAAGTATTGA ACAGCGACTC AAATGGGCAG GTGGTGCCAA CCCTGCACTG
    GCACCTGTAC TACAGGATTT TGAAGCAACA ATAGCTGAAA GAAGAAATCT TGTCCTTAAG
    GAGAGCCAAA GAGCAAGTCA GGTCACTTTC CTCTGCAGCA ATATCATTCA TTTTGAAAGT
    TTACGAACTA GAACTGCAGA AGCCTTAAAC CTGGATGCTG CATTATTTGA ACTAATCAAA
    CGGTGTCAGC AAATGTGTTC ATTTGCATCG CAGTTTAACA GTTCAGTCTC TGAGTTAGAG
    CTTCGTTTAT TACAGAGGGT GGACACGACT CTTGAACATC CTATTGGCAG CTCTGAGTGG
    CTTTTGTCAG CACACAAACA ACTGACCCAG GATATGTCTA CTCAGAGAGC AATTCAGACA
    GAGAAAGAGC AACAAATAGA AACAGTCTGT GAAACAATTC AGAATCTGGT TGATAATATA
    AAGACTGTGC TCACTGGCCA TAACCGGCAG CTTGGAGATG TCAAACATCT CCTGAAAGCA
    ATGGCCAAGG ATGAAGAAGC TGCTCTGGCA GATGGTGAAG ATGTTCCCTA TGAGAACAGT
    GTTAGGCAGT TCTTGGGTGA ATATAAATCA TGGCAAGACA ACATTCAGAC AGTGCTGTTT
    ACATTAGTCC AAGCCATGGG TCAGGTTCGA AGTCAAGAAC ATGTTGAAAT GCTCCAGGAA
    ATAACTCCCA CCTTGAAAGA ACTGAAAACG CAAAGTCAGA GTGTGTATAA TAATTTAGTG
    AGTTTTGCAT CACCATTAGT GACCGATGCA ACAAATGAAT GTTCGAGTCC AACATCGTCT
    GCTACTTATC AACCATCCTT TGCTGCAGCA GTCCGAAGCA ACACTGGCCA GAAGACTCAG
    CCTGATGTCA TGTCACAGAA CGCTAGAAAG CTGATTCAGA AAAACCTAGC TACATCAGCA
    GATACCCCAC CGAGCACCGT TCCGGGAACT GGCAAGAGTG TTGCTTGTAG TCCTAAAAAG
    GCCATCAGAG ACCCTAAGAC TGGAAAAGCT GTGCAGGAGA GAAACTCGTA TGCGGTGAGT
    GTGTGGAAGA GAGTGAAAGC CAAGCTAGAG GGCCGAGATG TTGATCCGAA TAGGAGGATG
    TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA CTAATCTAGA TAACTTGGCT
    CAGCTGTATG AAGGTTGGAC AGCCTGGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.