ACTN1 cDNA ORF clone, Bos taurus(cattle)

The following ACTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa44903 NM_001035351.2
Latest version!
Bos taurus actinin alpha 1 (ACTN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa23483 XM_005211976.2
Latest version!
Bos taurus actinin alpha 1 (ACTN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa23485 XM_005211978.3
Latest version!
Bos taurus actinin alpha 1 (ACTN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa23486 XM_005211979.2
Latest version!
Bos taurus actinin alpha 1 (ACTN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa50922 XM_010809468.2
Latest version!
Bos taurus actinin alpha 1 (ACTN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa50922 XM_010809468.1 Bos taurus actinin alpha 1 (ACTN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa23486 XM_005211979.1 Bos taurus actinin alpha 1 (ACTN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa23483 XM_005211976.1 Bos taurus actinin alpha 1 (ACTN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa23485 XM_005211978.2 Bos taurus actinin alpha 1 (ACTN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa44903
    Clone ID Related Accession (Same CDS sequence) NM_001035351.2
    Accession Version NM_001035351.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Bos taurus(cattle)
    Product alpha-actinin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107533.1. On Aug 30, 2012 this sequence version replaced NM_001035351.1. ##Evidence-Data-START## Transcript exon combination :: BC107533.1, EF512630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145436 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT
    GATATTGGCA ACGACCCCCA GGGAGAGGCA GAGTTTGCCC GCATCATGAG CATCGTGGAC
    CCCAACCGCC TGGGGGTGGT GACGTTCCAG GCCTTCATCG ACTTTATGTC TCGTGAGACA
    GCTGATACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC CGGGGATAAG
    AACTACATCA CGGTGGACGA GCTGCGCAGA GAGCTGCCGC CTGACCAGGC TGAGTACTGC
    ATCGCGCGAA TGGCCCCCTA CACCGGCCCC GACGCCGTGC CCGGTGCTCT GGACTACATG
    TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001030428.1
    CDS279..2957
    Translation

    Target ORF information:

    RefSeq Version NM_001035351.2
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001035351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT
    GATATTGGCA ACGACCCCCA GGGAGAGGCA GAGTTTGCCC GCATCATGAG CATCGTGGAC
    CCCAACCGCC TGGGGGTGGT GACGTTCCAG GCCTTCATCG ACTTTATGTC TCGTGAGACA
    GCTGATACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC CGGGGATAAG
    AACTACATCA CGGTGGACGA GCTGCGCAGA GAGCTGCCGC CTGACCAGGC TGAGTACTGC
    ATCGCGCGAA TGGCCCCCTA CACCGGCCCC GACGCCGTGC CCGGTGCTCT GGACTACATG
    TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23483
    Clone ID Related Accession (Same CDS sequence) XM_005211976.1 , XM_005211976.2
    Accession Version XM_005211976.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT
    GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC
    TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC
    GTGGACCCCA ACCGCCTGGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGT
    GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG
    GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCGCCTGA CCAGGCTGAG
    TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC
    TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212033.1
    CDS415..3159
    Translation

    Target ORF information:

    RefSeq Version XM_005211976.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT
    GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC
    TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC
    GTGGACCCCA ACCGCCTGGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGT
    GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG
    GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCGCCTGA CCAGGCTGAG
    TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC
    TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23483
    Clone ID Related Accession (Same CDS sequence) XM_005211976.1 , XM_005211976.2
    Accession Version XM_005211976.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT
    GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC
    TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC
    GTGGACCCCA ACCGCCTGGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGT
    GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG
    GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCGCCTGA CCAGGCTGAG
    TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC
    TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212033.1
    CDS415..3159
    Translation

    Target ORF information:

    RefSeq Version XM_005211976.2
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211976.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT
    GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC
    TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC
    GTGGACCCCA ACCGCCTGGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGT
    GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG
    GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCGCCTGA CCAGGCTGAG
    TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC
    TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23485
    Clone ID Related Accession (Same CDS sequence) XM_005211978.3 , XM_005211978.2
    Accession Version XM_005211978.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211978.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTTATCTC CATGGGTTAC
    AACATGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212035.1
    CDS336..2999
    Translation

    Target ORF information:

    RefSeq Version XM_005211978.3
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211978.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTTATCTC CATGGGTTAC
    AACATGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23485
    Clone ID Related Accession (Same CDS sequence) XM_005211978.3 , XM_005211978.2
    Accession Version XM_005211978.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTTATCTC CATGGGTTAC
    AACATGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212035.1
    CDS1..2664
    Translation

    Target ORF information:

    RefSeq Version XM_005211978.2
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211978.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTTATCTC CATGGGTTAC
    AACATGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23486
    Clone ID Related Accession (Same CDS sequence) XM_005211979.2 , XM_005211979.1
    Accession Version XM_005211979.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC
    ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTGGGGGTG
    GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGTGAGA CAGCTGATAC AGATACAGCA
    GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC
    GAGCTGCGCA GAGAGCTGCC GCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC
    TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG
    TACGGCGAGA GTGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212036.1
    CDS415..3075
    Translation

    Target ORF information:

    RefSeq Version XM_005211979.2
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211979.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC
    ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTGGGGGTG
    GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGTGAGA CAGCTGATAC AGATACAGCA
    GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC
    GAGCTGCGCA GAGAGCTGCC GCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC
    TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG
    TACGGCGAGA GTGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23486
    Clone ID Related Accession (Same CDS sequence) XM_005211979.2 , XM_005211979.1
    Accession Version XM_005211979.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC
    ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTGGGGGTG
    GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGTGAGA CAGCTGATAC AGATACAGCA
    GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC
    GAGCTGCGCA GAGAGCTGCC GCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC
    TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG
    TACGGCGAGA GTGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212036.1
    CDS415..3075
    Translation

    Target ORF information:

    RefSeq Version XM_005211979.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
    CGAGACCTGC TCTTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG
    TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG
    GACGGCCTGA AGCTGATGCT ACTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC
    GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCCT GGACTTCATA
    GCCAGCAAGG GGGTCAAGCT GGTGTCCATA GGAGCTGAAG AAATCGTGGA TGGGAATGTG
    AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCT
    GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT
    TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT
    GCCTTGATCC ACCGACACCG GCCTGAGCTG ATCGACTATG GGAAGCTGCG GAAGGATGAC
    CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG
    ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCGG ACGAGAAAGC CATCATGACT
    TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT
    CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG
    AAGCTGGCCA GCGACCTGCT GGAGTGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG
    GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG
    CGCCTTCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG
    CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCCGA GGGCAGGATG
    GTCTCGGACA TCAACAACGC ATGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG
    TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGG
    CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG
    GATTACGAGA CGGCTACCCT CTCGGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT
    GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG
    CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT
    GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG
    GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCTGCACCC
    TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT CGTGCACACC
    ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAGGCCAC CCTCCCCGAT
    GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG
    ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC
    AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTGACGGAG
    GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC TCAGGCCAAT
    GTCATCGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG
    CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ATGAGAAGAG CATCGTCAAC
    TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TCATCCAGGA GGCGCTCATC
    TTCGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC
    CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC
    AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG
    AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC
    ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTGGGGGTG
    GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGTGAGA CAGCTGATAC AGATACAGCA
    GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC
    GAGCTGCGCA GAGAGCTGCC GCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC
    TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG
    TACGGCGAGA GTGACCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50922
    Clone ID Related Accession (Same CDS sequence) XM_010809468.2 , XM_010809468.1
    Accession Version XM_010809468.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACTGC TGGAGGTCAT CTCAGGTGAA CGTTTGGCCA AGCCCGAGAG AGGCAAAATG 
    AGGGTGCACA AGATCTCCAA TGTCAACAAG GCCCTGGACT TCATAGCCAG CAAGGGGGTC
    AAGCTGGTGT CCATAGGAGC TGAAGAAATC GTGGATGGGA ATGTGAAAAT GACCCTGGGC
    ATGATCTGGA CCATCATCCT CCGCTTTGCC ATCCAGGACA TCTCTGTGGA AGAGACTTCA
    GCGAAGGAAG GGCTTCTCTT GTGGTGTCAG AGAAAGACAG CCCCTTACAA GAATGTCAAC
    ATCCAGAACT TCCACATAAG CTGGAAGGAC GGCCTCGGCT TCTGTGCCTT GATCCACCGA
    CACCGGCCTG AGCTGATCGA CTATGGGAAG CTGCGGAAGG ATGACCCACT GACAAACTTG
    AACACAGCTT TCGATGTAGC GGAGAAGTAC CTGGACATTC CCAAGATGTT GGATGCGGAA
    GACATCGTCG GAACCGCCCG CCCGGACGAG AAAGCCATCA TGACTTACGT GTCCAGCTTC
    TACCACGCCT TCTCTGGAGC CCAGAAGGCC GAGACAGCAG CCAATCGCAT CTGCAAGGTG
    CTGGCCGTCA ACCAGGAGAA CGAGCAGCTC ATGGAGGACT ACGAGAAGCT GGCCAGCGAC
    CTGCTGGAGT GGATCCGCCG CACCATCCCA TGGCTGGAGA ACCGGGCACC CGAGAACACC
    ATGCACGCCA TGCAGCAGAA GCTGGAGGAC TTCCGGGACT ACCGGCGCCT TCACAAGCCG
    CCCAAGGTGC AGGAGAAGTG CCAGCTGGAG ATCAACTTCA ACACGCTGCA GACCAAGCTG
    CGCCTCAGCA ACCGGCCCGC CTTCATGCCC TCCGAGGGCA GGATGGTCTC GGACATCAAC
    AACGCATGGG GCTGCCTGGA GCAGGCGGAG AAGGGCTATG AGGAGTGGCT GCTGAATGAG
    ATCCGGAGGC TGGAGCGGCT CGACCACCTG GCAGAGAAGT TCCGGCAGAA GGCCTCCATC
    CACGAGGCCT GGACGGACGG CAAAGAGGCC ATGCTGCGGC AGAAGGATTA CGAGACGGCT
    ACCCTCTCGG AGATCAAGGC CCTGCTCAAG AAGCACGAGG CCTTTGAGAG TGACCTGGCT
    GCCCACCAGG ACCGCGTGGA GCAGATTGCC GCCATTGCCC AGGAGCTCAA TGAGCTGGAC
    TATTACGACT CACCCAGTGT CAACGCCCGG TGCCAAAAGA TCTGTGACCA GTGGGACAAT
    CTAGGGGCCC TAACCCAGAA GCGAAGGGAA GCTCTGGAGC GCACGGAGAA ACTGCTGGAG
    ACCATTGACC AACTGTACTT GGAGTACGCT AAGCGGGCTG CACCCTTCAA CAACTGGATG
    GAGGGGGCCA TGGAGGACCT GCAGGACACC TTCATCGTGC ACACCATCGA GGAGATCCAG
    GGACTGACCA CAGCCCATGA GCAGTTCAAG GCCACCCTCC CCGATGCCGA CAAGGAGCGC
    CTGGCTATCC TGGGCATCCA CAACGAGGTG TCCAAGATTG TCCAGACCTA CCACGTCAAC
    ATGGCAGGCA CCAACCCCTA CACAACCATC ACGCCTCAGG AGATCAATGG CAAATGGGAC
    CATGTGCGGC AGCTGGTGCC ACGGAGGGAC CAGGCCCTGA CGGAGGAGCA CGCCCGCCAG
    CAGCACAACG AAAGGCTACG CAAGCAGTTT GGGGCTCAGG CCAATGTCAT CGGGCCCTGG
    ATCCAGACCA AGATGGAGGA GATTGGAAGG ATCTCCATTG AGATGCACGG GACCCTGGAG
    GACCAGCTCA ACCACCTGCG GCAATATGAG AAGAGCATCG TCAACTACAA GCCCAAGATC
    GACCAGCTGG AGGGCGACCA CCAGCTCATC CAGGAGGCGC TCATCTTCGA CAACAAGCAC
    ACCAACTACA CCATGGAGCA CATCCGTGTG GGCTGGGAGC AGCTCCTAAC CACCATCGCC
    AGGACCATCA ATGAGGTGGA GAACCAGATC CTGACCAGGG ATGCCAAGGG CATCAGCCAG
    GAGCAGATGA ATGAGTTCCG GGCCTCCTTC AACCACTTTG ACCGGGATCA CTCCGGCACG
    CTGGGTCCCG AGGAGTTCAA AGCCTGCCTC ATCAGCTTGG GTTATGATAT TGGCAACGAC
    CCCCAGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807770.1
    CDS187..2670
    Translation

    Target ORF information:

    RefSeq Version XM_010809468.2
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809468.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACTGC TGGAGGTCAT CTCAGGTGAA CGTTTGGCCA AGCCCGAGAG AGGCAAAATG 
    AGGGTGCACA AGATCTCCAA TGTCAACAAG GCCCTGGACT TCATAGCCAG CAAGGGGGTC
    AAGCTGGTGT CCATAGGAGC TGAAGAAATC GTGGATGGGA ATGTGAAAAT GACCCTGGGC
    ATGATCTGGA CCATCATCCT CCGCTTTGCC ATCCAGGACA TCTCTGTGGA AGAGACTTCA
    GCGAAGGAAG GGCTTCTCTT GTGGTGTCAG AGAAAGACAG CCCCTTACAA GAATGTCAAC
    ATCCAGAACT TCCACATAAG CTGGAAGGAC GGCCTCGGCT TCTGTGCCTT GATCCACCGA
    CACCGGCCTG AGCTGATCGA CTATGGGAAG CTGCGGAAGG ATGACCCACT GACAAACTTG
    AACACAGCTT TCGATGTAGC GGAGAAGTAC CTGGACATTC CCAAGATGTT GGATGCGGAA
    GACATCGTCG GAACCGCCCG CCCGGACGAG AAAGCCATCA TGACTTACGT GTCCAGCTTC
    TACCACGCCT TCTCTGGAGC CCAGAAGGCC GAGACAGCAG CCAATCGCAT CTGCAAGGTG
    CTGGCCGTCA ACCAGGAGAA CGAGCAGCTC ATGGAGGACT ACGAGAAGCT GGCCAGCGAC
    CTGCTGGAGT GGATCCGCCG CACCATCCCA TGGCTGGAGA ACCGGGCACC CGAGAACACC
    ATGCACGCCA TGCAGCAGAA GCTGGAGGAC TTCCGGGACT ACCGGCGCCT TCACAAGCCG
    CCCAAGGTGC AGGAGAAGTG CCAGCTGGAG ATCAACTTCA ACACGCTGCA GACCAAGCTG
    CGCCTCAGCA ACCGGCCCGC CTTCATGCCC TCCGAGGGCA GGATGGTCTC GGACATCAAC
    AACGCATGGG GCTGCCTGGA GCAGGCGGAG AAGGGCTATG AGGAGTGGCT GCTGAATGAG
    ATCCGGAGGC TGGAGCGGCT CGACCACCTG GCAGAGAAGT TCCGGCAGAA GGCCTCCATC
    CACGAGGCCT GGACGGACGG CAAAGAGGCC ATGCTGCGGC AGAAGGATTA CGAGACGGCT
    ACCCTCTCGG AGATCAAGGC CCTGCTCAAG AAGCACGAGG CCTTTGAGAG TGACCTGGCT
    GCCCACCAGG ACCGCGTGGA GCAGATTGCC GCCATTGCCC AGGAGCTCAA TGAGCTGGAC
    TATTACGACT CACCCAGTGT CAACGCCCGG TGCCAAAAGA TCTGTGACCA GTGGGACAAT
    CTAGGGGCCC TAACCCAGAA GCGAAGGGAA GCTCTGGAGC GCACGGAGAA ACTGCTGGAG
    ACCATTGACC AACTGTACTT GGAGTACGCT AAGCGGGCTG CACCCTTCAA CAACTGGATG
    GAGGGGGCCA TGGAGGACCT GCAGGACACC TTCATCGTGC ACACCATCGA GGAGATCCAG
    GGACTGACCA CAGCCCATGA GCAGTTCAAG GCCACCCTCC CCGATGCCGA CAAGGAGCGC
    CTGGCTATCC TGGGCATCCA CAACGAGGTG TCCAAGATTG TCCAGACCTA CCACGTCAAC
    ATGGCAGGCA CCAACCCCTA CACAACCATC ACGCCTCAGG AGATCAATGG CAAATGGGAC
    CATGTGCGGC AGCTGGTGCC ACGGAGGGAC CAGGCCCTGA CGGAGGAGCA CGCCCGCCAG
    CAGCACAACG AAAGGCTACG CAAGCAGTTT GGGGCTCAGG CCAATGTCAT CGGGCCCTGG
    ATCCAGACCA AGATGGAGGA GATTGGAAGG ATCTCCATTG AGATGCACGG GACCCTGGAG
    GACCAGCTCA ACCACCTGCG GCAATATGAG AAGAGCATCG TCAACTACAA GCCCAAGATC
    GACCAGCTGG AGGGCGACCA CCAGCTCATC CAGGAGGCGC TCATCTTCGA CAACAAGCAC
    ACCAACTACA CCATGGAGCA CATCCGTGTG GGCTGGGAGC AGCTCCTAAC CACCATCGCC
    AGGACCATCA ATGAGGTGGA GAACCAGATC CTGACCAGGG ATGCCAAGGG CATCAGCCAG
    GAGCAGATGA ATGAGTTCCG GGCCTCCTTC AACCACTTTG ACCGGGATCA CTCCGGCACG
    CTGGGTCCCG AGGAGTTCAA AGCCTGCCTC ATCAGCTTGG GTTATGATAT TGGCAACGAC
    CCCCAGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50922
    Clone ID Related Accession (Same CDS sequence) XM_010809468.2 , XM_010809468.1
    Accession Version XM_010809468.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-actinin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104283.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACTGC TGGAGGTCAT CTCAGGTGAA CGTTTGGCCA AGCCCGAGAG AGGCAAAATG 
    AGGGTGCACA AGATCTCCAA TGTCAACAAG GCCCTGGACT TCATAGCCAG CAAGGGGGTC
    AAGCTGGTGT CCATAGGAGC TGAAGAAATC GTGGATGGGA ATGTGAAAAT GACCCTGGGC
    ATGATCTGGA CCATCATCCT CCGCTTTGCC ATCCAGGACA TCTCTGTGGA AGAGACTTCA
    GCGAAGGAAG GGCTTCTCTT GTGGTGTCAG AGAAAGACAG CCCCTTACAA GAATGTCAAC
    ATCCAGAACT TCCACATAAG CTGGAAGGAC GGCCTCGGCT TCTGTGCCTT GATCCACCGA
    CACCGGCCTG AGCTGATCGA CTATGGGAAG CTGCGGAAGG ATGACCCACT GACAAACTTG
    AACACAGCTT TCGATGTAGC GGAGAAGTAC CTGGACATTC CCAAGATGTT GGATGCGGAA
    GACATCGTCG GAACCGCCCG CCCGGACGAG AAAGCCATCA TGACTTACGT GTCCAGCTTC
    TACCACGCCT TCTCTGGAGC CCAGAAGGCC GAGACAGCAG CCAATCGCAT CTGCAAGGTG
    CTGGCCGTCA ACCAGGAGAA CGAGCAGCTC ATGGAGGACT ACGAGAAGCT GGCCAGCGAC
    CTGCTGGAGT GGATCCGCCG CACCATCCCA TGGCTGGAGA ACCGGGCACC CGAGAACACC
    ATGCACGCCA TGCAGCAGAA GCTGGAGGAC TTCCGGGACT ACCGGCGCCT TCACAAGCCG
    CCCAAGGTGC AGGAGAAGTG CCAGCTGGAG ATCAACTTCA ACACGCTGCA GACCAAGCTG
    CGCCTCAGCA ACCGGCCCGC CTTCATGCCC TCCGAGGGCA GGATGGTCTC GGACATCAAC
    AACGCATGGG GCTGCCTGGA GCAGGCGGAG AAGGGCTATG AGGAGTGGCT GCTGAATGAG
    ATCCGGAGGC TGGAGCGGCT CGACCACCTG GCAGAGAAGT TCCGGCAGAA GGCCTCCATC
    CACGAGGCCT GGACGGACGG CAAAGAGGCC ATGCTGCGGC AGAAGGATTA CGAGACGGCT
    ACCCTCTCGG AGATCAAGGC CCTGCTCAAG AAGCACGAGG CCTTTGAGAG TGACCTGGCT
    GCCCACCAGG ACCGCGTGGA GCAGATTGCC GCCATTGCCC AGGAGCTCAA TGAGCTGGAC
    TATTACGACT CACCCAGTGT CAACGCCCGG TGCCAAAAGA TCTGTGACCA GTGGGACAAT
    CTAGGGGCCC TAACCCAGAA GCGAAGGGAA GCTCTGGAGC GCACGGAGAA ACTGCTGGAG
    ACCATTGACC AACTGTACTT GGAGTACGCT AAGCGGGCTG CACCCTTCAA CAACTGGATG
    GAGGGGGCCA TGGAGGACCT GCAGGACACC TTCATCGTGC ACACCATCGA GGAGATCCAG
    GGACTGACCA CAGCCCATGA GCAGTTCAAG GCCACCCTCC CCGATGCCGA CAAGGAGCGC
    CTGGCTATCC TGGGCATCCA CAACGAGGTG TCCAAGATTG TCCAGACCTA CCACGTCAAC
    ATGGCAGGCA CCAACCCCTA CACAACCATC ACGCCTCAGG AGATCAATGG CAAATGGGAC
    CATGTGCGGC AGCTGGTGCC ACGGAGGGAC CAGGCCCTGA CGGAGGAGCA CGCCCGCCAG
    CAGCACAACG AAAGGCTACG CAAGCAGTTT GGGGCTCAGG CCAATGTCAT CGGGCCCTGG
    ATCCAGACCA AGATGGAGGA GATTGGAAGG ATCTCCATTG AGATGCACGG GACCCTGGAG
    GACCAGCTCA ACCACCTGCG GCAATATGAG AAGAGCATCG TCAACTACAA GCCCAAGATC
    GACCAGCTGG AGGGCGACCA CCAGCTCATC CAGGAGGCGC TCATCTTCGA CAACAAGCAC
    ACCAACTACA CCATGGAGCA CATCCGTGTG GGCTGGGAGC AGCTCCTAAC CACCATCGCC
    AGGACCATCA ATGAGGTGGA GAACCAGATC CTGACCAGGG ATGCCAAGGG CATCAGCCAG
    GAGCAGATGA ATGAGTTCCG GGCCTCCTTC AACCACTTTG ACCGGGATCA CTCCGGCACG
    CTGGGTCCCG AGGAGTTCAA AGCCTGCCTC ATCAGCTTGG GTTATGATAT TGGCAACGAC
    CCCCAGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807770.1
    CDS187..2670
    Translation

    Target ORF information:

    RefSeq Version XM_010809468.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809468.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTACTGC TGGAGGTCAT CTCAGGTGAA CGTTTGGCCA AGCCCGAGAG AGGCAAAATG 
    AGGGTGCACA AGATCTCCAA TGTCAACAAG GCCCTGGACT TCATAGCCAG CAAGGGGGTC
    AAGCTGGTGT CCATAGGAGC TGAAGAAATC GTGGATGGGA ATGTGAAAAT GACCCTGGGC
    ATGATCTGGA CCATCATCCT CCGCTTTGCC ATCCAGGACA TCTCTGTGGA AGAGACTTCA
    GCGAAGGAAG GGCTTCTCTT GTGGTGTCAG AGAAAGACAG CCCCTTACAA GAATGTCAAC
    ATCCAGAACT TCCACATAAG CTGGAAGGAC GGCCTCGGCT TCTGTGCCTT GATCCACCGA
    CACCGGCCTG AGCTGATCGA CTATGGGAAG CTGCGGAAGG ATGACCCACT GACAAACTTG
    AACACAGCTT TCGATGTAGC GGAGAAGTAC CTGGACATTC CCAAGATGTT GGATGCGGAA
    GACATCGTCG GAACCGCCCG CCCGGACGAG AAAGCCATCA TGACTTACGT GTCCAGCTTC
    TACCACGCCT TCTCTGGAGC CCAGAAGGCC GAGACAGCAG CCAATCGCAT CTGCAAGGTG
    CTGGCCGTCA ACCAGGAGAA CGAGCAGCTC ATGGAGGACT ACGAGAAGCT GGCCAGCGAC
    CTGCTGGAGT GGATCCGCCG CACCATCCCA TGGCTGGAGA ACCGGGCACC CGAGAACACC
    ATGCACGCCA TGCAGCAGAA GCTGGAGGAC TTCCGGGACT ACCGGCGCCT TCACAAGCCG
    CCCAAGGTGC AGGAGAAGTG CCAGCTGGAG ATCAACTTCA ACACGCTGCA GACCAAGCTG
    CGCCTCAGCA ACCGGCCCGC CTTCATGCCC TCCGAGGGCA GGATGGTCTC GGACATCAAC
    AACGCATGGG GCTGCCTGGA GCAGGCGGAG AAGGGCTATG AGGAGTGGCT GCTGAATGAG
    ATCCGGAGGC TGGAGCGGCT CGACCACCTG GCAGAGAAGT TCCGGCAGAA GGCCTCCATC
    CACGAGGCCT GGACGGACGG CAAAGAGGCC ATGCTGCGGC AGAAGGATTA CGAGACGGCT
    ACCCTCTCGG AGATCAAGGC CCTGCTCAAG AAGCACGAGG CCTTTGAGAG TGACCTGGCT
    GCCCACCAGG ACCGCGTGGA GCAGATTGCC GCCATTGCCC AGGAGCTCAA TGAGCTGGAC
    TATTACGACT CACCCAGTGT CAACGCCCGG TGCCAAAAGA TCTGTGACCA GTGGGACAAT
    CTAGGGGCCC TAACCCAGAA GCGAAGGGAA GCTCTGGAGC GCACGGAGAA ACTGCTGGAG
    ACCATTGACC AACTGTACTT GGAGTACGCT AAGCGGGCTG CACCCTTCAA CAACTGGATG
    GAGGGGGCCA TGGAGGACCT GCAGGACACC TTCATCGTGC ACACCATCGA GGAGATCCAG
    GGACTGACCA CAGCCCATGA GCAGTTCAAG GCCACCCTCC CCGATGCCGA CAAGGAGCGC
    CTGGCTATCC TGGGCATCCA CAACGAGGTG TCCAAGATTG TCCAGACCTA CCACGTCAAC
    ATGGCAGGCA CCAACCCCTA CACAACCATC ACGCCTCAGG AGATCAATGG CAAATGGGAC
    CATGTGCGGC AGCTGGTGCC ACGGAGGGAC CAGGCCCTGA CGGAGGAGCA CGCCCGCCAG
    CAGCACAACG AAAGGCTACG CAAGCAGTTT GGGGCTCAGG CCAATGTCAT CGGGCCCTGG
    ATCCAGACCA AGATGGAGGA GATTGGAAGG ATCTCCATTG AGATGCACGG GACCCTGGAG
    GACCAGCTCA ACCACCTGCG GCAATATGAG AAGAGCATCG TCAACTACAA GCCCAAGATC
    GACCAGCTGG AGGGCGACCA CCAGCTCATC CAGGAGGCGC TCATCTTCGA CAACAAGCAC
    ACCAACTACA CCATGGAGCA CATCCGTGTG GGCTGGGAGC AGCTCCTAAC CACCATCGCC
    AGGACCATCA ATGAGGTGGA GAACCAGATC CTGACCAGGG ATGCCAAGGG CATCAGCCAG
    GAGCAGATGA ATGAGTTCCG GGCCTCCTTC AACCACTTTG ACCGGGATCA CTCCGGCACG
    CTGGGTCCCG AGGAGTTCAA AGCCTGCCTC ATCAGCTTGG GTTATGATAT TGGCAACGAC
    CCCCAGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTGGGG
    GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGTG AGACAGCTGA TACAGATACA
    GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG
    GACGAGCTGC GCAGAGAGCT GCCGCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC
    CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG
    CTGTACGGCG AGAGTGACCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.