CSPP1 cDNA ORF clone, Bos taurus(cattle)

The following CSPP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSPP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa222313 XM_025001589.1
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222314 XM_025001591.1
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222315 XM_025001592.1
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222316 XM_025001593.1
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa25976 NM_001193015.2
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa25976 XM_025001590.1
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa25976 XM_005215486.3
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa25976 XM_005215485.4
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50841 XM_005215479.4
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50842 XM_005215480.4
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50843 XM_005215481.3
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50844 XM_005215482.4
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50845 XM_010812038.3
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50846 XM_005215484.4
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50847 XM_005215487.4
Latest version!
Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa50844 XM_005215482.3 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa25976 XM_005215485.3 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50841 XM_005215479.3 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50845 XM_010812038.2 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50846 XM_005215484.3 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50842 XM_005215480.3 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50843 XM_005215481.2 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa25976 XM_005215486.2 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50847 XM_005215487.3 Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa222313
    Clone ID Related Accession (Same CDS sequence) XM_025001589.1
    Accession Version XM_025001589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCCTC CGGGGCGGCA GGGGGCGCCC CCGGCCGCGA GCCTCTGCCC AGCGGCCGCA 
    GTGGGGCGGG GCCACGCCCG GCCGTTGGCG AAGGGCGGGC GGAGGGCTCG GCCTGAGAGG
    CGGAGCCCGG GTGGCTTGTC ACACCGTTCC CGCGCTGGGC GACTGCCGTC GCTTCTTCGG
    ACCGCGGCTA AGAGCCTCGC TAGAACGCTG TGCTGTTCCC CGACGCCAGA CCGCTTAGCT
    GATATCTTCA AAATGGCTGA TAATTTGGAT GAATTTATTG AAGAGCGAAA AGTCAGATTG
    GCCAGAGACA AAGCAGAATT GGAAAGTGAT CCACCGTACA TGGAAATGAA GGGAAAAGCA
    TCAGAGAAGC TTTCTGAAAA CAGTAAAATA TTAATTTCCA TGGCTAAGGA AAACATACCA
    CCTAACAGTC AACAGACAAG GCGTTCTTTA GGAATTGATT ATGGATTAAG TTTACCACTT
    GGGGAAGAGT ATGAACAGAA GAAACATAAA CTGAAAGAAG AATTGCGACA AGATTACAGA
    CGCTATCTTA CTCAGGAAAG GTTGAAACTT GAACGTAACA AAGAATACAA TCAATTTCTC
    AGGGATAAGG AAGAATCCAC TGAAAAGTTC AGACAGGTAG AAAAGAGTAC TGAGCAGAAG
    AATCAAAGAA GTAAAAAACC TATCAGTCAG GTTAAACCTG ATTTACCTTC ACAAATACAA
    TCATCTTTCG AAAATTCAGA GGGTCCTAGA AAAGATGTCT TAACTCCTTC AGAGGCATAT
    GAAGAACTTC TAAACCAAAG ACGACTAGAA GAGGACAGGT ACCGAAAATT AGATGATGAA
    ATTGAATTAA GGAACAATAG AAGAGTCATT AAAAAGACTG ATGAAGAAGT GGGCGTTTCC
    AGTAAAAAAC ATCAAAGGTT TGACAGCACG ACTGACATTC CAGATAGAAG ATTTCACAGG
    TTTAATGAGG ATCTTGCTTT TGATAAACAG TCTTACAGAC CAGGCTATGA TCCAGAAGTA
    AGTGAAGACA TGGATGAGAG GTTTAGATAT GAAAGTGATT TTGATAGAAG ACTTTTGAGA
    GTTTATACAA AAAACAGGAT GTACGGGAAC AGACGAGGCA ATGAGCCTCC TATGGAGTGT
    GATGGTGATG TCACAGAACA GTCAAACATA CGAATTTCAT CTGCTGGAAA TCAAAGTGCT
    CGAGACAATG AACCATCCAA ACCTACTTAT CGAGAGACAT GCAGTCCTTT TGCGGGAATG
    CTCTTTGGGG GTGAAGATCG AGAACTTATT CAGAGAAGGA AAGAGAAATA TAGACAAGAA
    CTATTGGAAC AAATGGCTGA ACAACAGAGG AACAAGAGAC GAGAAAAAGA TTTGGATCTC
    AGGGTTGCAG CTTCTGGAGC ACAAGACCCT GAGAAATCGC CTGATAGACT AAAGCAGTTT
    AGTGTGGCAC CAAGACACTT TGAAGAGATG ATACCACCTG AGAGACCCAG AATAGCTTTC
    CAGACACCTC TCCCTCCTCT GTCCGCCCCA TCTGTCCCAC CCATCCCATC AGTTTACTCC
    ATCCCATCTC AGAGTGAAGA TGTGCACAGT GGACTCAGCA GCACTCTTGG TGACATGGTG
    CCTCCCAGAG TTGCACCTCT GCCTCCACCT CCCATGCTGC CCCCTTTGGC TGCGAACTAC
    CGAACTCCTT ACGATGACGC GTACTATTTC TATGGGGCCA GGAATACTTT GGATCCTAGT
    CTTGCTTATT ATGGTTCAGG GATGATGGGA GTACAGCCTG CTCCTGCTCC TTATGTTACA
    GCTCCTGTCA CCCACCAGCC AGTACAGCCT ATTGTGAGTA CAGTTGGACA GAATGAGCTG
    AAAATTACAA GTAATCGAGC AATAAATTCA GGATTGTTTT TTGAAGATAA ACCAAAGCCT
    TCAAAACAGT CATTTCAGTC TTACCAGGAA GCTTTGCAGC AGCAGATTCA AGAAAGACAA
    GAAAGAAGAA AGAAAGAACG TGAAGAAAAA GAAGTATATG AAGCCAAATT AGAAGCTGAA
    ATGAGAAGTT ACAACCCCTG GGGGAAAGGT GGAGGTGGTG CTCCTCTGAG AGATGACAAA
    GGAAATCTGA TAACTGATTT GAATAGGATG CACAGACAAA ATATAGATGC CTACCATAAC
    CCAGATGCAA GAGCATATGA AGATAAAAGG GCTGTTGTAT CTCTAGACCA AAATTTAGCC
    ACTTCAAATG CGGAGAACCA AGAAGATGCT GCAAATAAAA ACTCAGGTCA TATGCAATCA
    CAGAGCTCTC CTTTTGCTCG GGGAAATATT TTTAGTGAGC CTCCAACTGA ACTTCAAATT
    AAACAGCAGG AATTATACAA GAATTTTCTT CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA
    GAAGAAGCAG AGCGAGAGAG ATTAAGAATT GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA
    GAACAGAGAG CTCGAATCCA GCAAGAGTAT GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA
    GAGGAGGAGC AAAGACTGAA AAACGAAGAG CTTATTCGGT TAGCTGAAGA AAGACGAAAA
    GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA GAAAAACATA ATCTACAACT TCAGCACTAC
    TATGAAAGAG AAAACATGAT TGGGGAAGAT ACAAAGCACT TGAGACAGCC TTCTCCTGTG
    GTTCCTGCTC TTCAGAATAA GATTGCAAGC AGACTCCAAA GACCTCCTTC AGTTGACAGC
    ATTATAACTT CCTTTACTCA GGACAGTTCC ATGTCCATGG CACAATCTCC TCCGGTACCT
    GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG GAGAAAAAAA ATGTAATTAT GGAATTATCA
    GAAATGAGAA AACAGCTTCG TAGTGAAGAG CGACGTCTGC AGGGGCGGTT ACTACACATT
    GATAGTGACG ACGACATTCA TATAAGGAAA AGAGAAAGTT CCATGGATGT ATTTGATATG
    GCCAGACATC GATTACAAGC TCCTGTCAGA AGACCGTCAC CTAAGAGCTT AGACTCTGCT
    TCTTCTCAGA ATATTCATGA CTTTAAGGAG CTGAAAGATA GAGATTCAGA AACGCGAGCT
    GATCTGAAAT CTATGTACCC AGATGATCCA AGAGATAATG ACACCTTGGA GATAGAACAA
    CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA CTGAATAAAC TAAAAATGCA GGAAGTTGAC
    TTGGATGCTG TCCCAAGCAC TAAAGTACGG GACCACAGAA TGCCCAGAGA GGACACTGAT
    GATTTCTTGA AAAACTCATT ATTAGAATCT GATAGTGCTT TTATTGGTGC GCATGGCGAG
    ACGTACCCAG CCATTGAGGA TGGGGGCTTC CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA
    GAGCGCAGAA GGAACAAACT GAAAGGATTG GACTTTGATA ACAGTCACCC TGATGTGCCA
    CCAGATGGTG TCTCTTTAAA ATCTGTATCC AGTATAAATA TTGATCAGCT TAGAATGAGA
    AATGAAGAAC GAATGCGAAG ACTGAATGAA CTTCAGAATA AACCTATTAC TACAGATGAT
    GAGAGTTCAC TGGTGGACCC TGATGACATC ATGAGGCACC GGGGTGACGA CGGATCAAAC
    TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA GGCACCTCAG AAACACTGAA GCGATTCATG
    GTGGAACAGC TGAGGCAGGA GCAGCAGCAG GGTCCTGGCA AACCAGGCGC TTTCACCTGG
    CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857357.1
    CDS16..3822
    Translation

    Target ORF information:

    RefSeq Version XM_025001589.1
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCCTC CGGGGCGGCA GGGGGCGCCC CCGGCCGCGA GCCTCTGCCC AGCGGCCGCA 
    GTGGGGCGGG GCCACGCCCG GCCGTTGGCG AAGGGCGGGC GGAGGGCTCG GCCTGAGAGG
    CGGAGCCCGG GTGGCTTGTC ACACCGTTCC CGCGCTGGGC GACTGCCGTC GCTTCTTCGG
    ACCGCGGCTA AGAGCCTCGC TAGAACGCTG TGCTGTTCCC CGACGCCAGA CCGCTTAGCT
    GATATCTTCA AAATGGCTGA TAATTTGGAT GAATTTATTG AAGAGCGAAA AGTCAGATTG
    GCCAGAGACA AAGCAGAATT GGAAAGTGAT CCACCGTACA TGGAAATGAA GGGAAAAGCA
    TCAGAGAAGC TTTCTGAAAA CAGTAAAATA TTAATTTCCA TGGCTAAGGA AAACATACCA
    CCTAACAGTC AACAGACAAG GCGTTCTTTA GGAATTGATT ATGGATTAAG TTTACCACTT
    GGGGAAGAGT ATGAACAGAA GAAACATAAA CTGAAAGAAG AATTGCGACA AGATTACAGA
    CGCTATCTTA CTCAGGAAAG GTTGAAACTT GAACGTAACA AAGAATACAA TCAATTTCTC
    AGGGATAAGG AAGAATCCAC TGAAAAGTTC AGACAGGTAG AAAAGAGTAC TGAGCAGAAG
    AATCAAAGAA GTAAAAAACC TATCAGTCAG GTTAAACCTG ATTTACCTTC ACAAATACAA
    TCATCTTTCG AAAATTCAGA GGGTCCTAGA AAAGATGTCT TAACTCCTTC AGAGGCATAT
    GAAGAACTTC TAAACCAAAG ACGACTAGAA GAGGACAGGT ACCGAAAATT AGATGATGAA
    ATTGAATTAA GGAACAATAG AAGAGTCATT AAAAAGACTG ATGAAGAAGT GGGCGTTTCC
    AGTAAAAAAC ATCAAAGGTT TGACAGCACG ACTGACATTC CAGATAGAAG ATTTCACAGG
    TTTAATGAGG ATCTTGCTTT TGATAAACAG TCTTACAGAC CAGGCTATGA TCCAGAAGTA
    AGTGAAGACA TGGATGAGAG GTTTAGATAT GAAAGTGATT TTGATAGAAG ACTTTTGAGA
    GTTTATACAA AAAACAGGAT GTACGGGAAC AGACGAGGCA ATGAGCCTCC TATGGAGTGT
    GATGGTGATG TCACAGAACA GTCAAACATA CGAATTTCAT CTGCTGGAAA TCAAAGTGCT
    CGAGACAATG AACCATCCAA ACCTACTTAT CGAGAGACAT GCAGTCCTTT TGCGGGAATG
    CTCTTTGGGG GTGAAGATCG AGAACTTATT CAGAGAAGGA AAGAGAAATA TAGACAAGAA
    CTATTGGAAC AAATGGCTGA ACAACAGAGG AACAAGAGAC GAGAAAAAGA TTTGGATCTC
    AGGGTTGCAG CTTCTGGAGC ACAAGACCCT GAGAAATCGC CTGATAGACT AAAGCAGTTT
    AGTGTGGCAC CAAGACACTT TGAAGAGATG ATACCACCTG AGAGACCCAG AATAGCTTTC
    CAGACACCTC TCCCTCCTCT GTCCGCCCCA TCTGTCCCAC CCATCCCATC AGTTTACTCC
    ATCCCATCTC AGAGTGAAGA TGTGCACAGT GGACTCAGCA GCACTCTTGG TGACATGGTG
    CCTCCCAGAG TTGCACCTCT GCCTCCACCT CCCATGCTGC CCCCTTTGGC TGCGAACTAC
    CGAACTCCTT ACGATGACGC GTACTATTTC TATGGGGCCA GGAATACTTT GGATCCTAGT
    CTTGCTTATT ATGGTTCAGG GATGATGGGA GTACAGCCTG CTCCTGCTCC TTATGTTACA
    GCTCCTGTCA CCCACCAGCC AGTACAGCCT ATTGTGAGTA CAGTTGGACA GAATGAGCTG
    AAAATTACAA GTAATCGAGC AATAAATTCA GGATTGTTTT TTGAAGATAA ACCAAAGCCT
    TCAAAACAGT CATTTCAGTC TTACCAGGAA GCTTTGCAGC AGCAGATTCA AGAAAGACAA
    GAAAGAAGAA AGAAAGAACG TGAAGAAAAA GAAGTATATG AAGCCAAATT AGAAGCTGAA
    ATGAGAAGTT ACAACCCCTG GGGGAAAGGT GGAGGTGGTG CTCCTCTGAG AGATGACAAA
    GGAAATCTGA TAACTGATTT GAATAGGATG CACAGACAAA ATATAGATGC CTACCATAAC
    CCAGATGCAA GAGCATATGA AGATAAAAGG GCTGTTGTAT CTCTAGACCA AAATTTAGCC
    ACTTCAAATG CGGAGAACCA AGAAGATGCT GCAAATAAAA ACTCAGGTCA TATGCAATCA
    CAGAGCTCTC CTTTTGCTCG GGGAAATATT TTTAGTGAGC CTCCAACTGA ACTTCAAATT
    AAACAGCAGG AATTATACAA GAATTTTCTT CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA
    GAAGAAGCAG AGCGAGAGAG ATTAAGAATT GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA
    GAACAGAGAG CTCGAATCCA GCAAGAGTAT GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA
    GAGGAGGAGC AAAGACTGAA AAACGAAGAG CTTATTCGGT TAGCTGAAGA AAGACGAAAA
    GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA GAAAAACATA ATCTACAACT TCAGCACTAC
    TATGAAAGAG AAAACATGAT TGGGGAAGAT ACAAAGCACT TGAGACAGCC TTCTCCTGTG
    GTTCCTGCTC TTCAGAATAA GATTGCAAGC AGACTCCAAA GACCTCCTTC AGTTGACAGC
    ATTATAACTT CCTTTACTCA GGACAGTTCC ATGTCCATGG CACAATCTCC TCCGGTACCT
    GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG GAGAAAAAAA ATGTAATTAT GGAATTATCA
    GAAATGAGAA AACAGCTTCG TAGTGAAGAG CGACGTCTGC AGGGGCGGTT ACTACACATT
    GATAGTGACG ACGACATTCA TATAAGGAAA AGAGAAAGTT CCATGGATGT ATTTGATATG
    GCCAGACATC GATTACAAGC TCCTGTCAGA AGACCGTCAC CTAAGAGCTT AGACTCTGCT
    TCTTCTCAGA ATATTCATGA CTTTAAGGAG CTGAAAGATA GAGATTCAGA AACGCGAGCT
    GATCTGAAAT CTATGTACCC AGATGATCCA AGAGATAATG ACACCTTGGA GATAGAACAA
    CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA CTGAATAAAC TAAAAATGCA GGAAGTTGAC
    TTGGATGCTG TCCCAAGCAC TAAAGTACGG GACCACAGAA TGCCCAGAGA GGACACTGAT
    GATTTCTTGA AAAACTCATT ATTAGAATCT GATAGTGCTT TTATTGGTGC GCATGGCGAG
    ACGTACCCAG CCATTGAGGA TGGGGGCTTC CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA
    GAGCGCAGAA GGAACAAACT GAAAGGATTG GACTTTGATA ACAGTCACCC TGATGTGCCA
    CCAGATGGTG TCTCTTTAAA ATCTGTATCC AGTATAAATA TTGATCAGCT TAGAATGAGA
    AATGAAGAAC GAATGCGAAG ACTGAATGAA CTTCAGAATA AACCTATTAC TACAGATGAT
    GAGAGTTCAC TGGTGGACCC TGATGACATC ATGAGGCACC GGGGTGACGA CGGATCAAAC
    TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA GGCACCTCAG AAACACTGAA GCGATTCATG
    GTGGAACAGC TGAGGCAGGA GCAGCAGCAG GGTCCTGGCA AACCAGGCGC TTTCACCTGG
    CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222314
    Clone ID Related Accession (Same CDS sequence) XM_025001591.1
    Accession Version XM_025001591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGCCTC CGGGGCGGCA GGGGGCGCCC CCGGCCGCGA GCCTCTGCCC AGCGGCCGCA 
    GTGGGGCGGG GCCACGCCCG GCCGTTGGCG AAGGGCGGGC GGAGGGCTCG GCCTGAGAGG
    CGGAGCCCGG GTGGCTTGTC ACACCGTTCC CGCGCTGGGC GACTGCCGTC GCTTCTTCGG
    ACCGCGGCTA AGAGCCTCGC TAGAACGCTG TGCTGTTCCC CGACGCCAGA CCGCTTAGCT
    GATATCTTCA AAATGGCTGA TAATTTGGAT GAATTTATTG AAGAGCGAAA AGTCAGATTG
    GCCAGAGACA AAGCAGAATT GGAAAGTGAT CCACCGTACA TGGAAATGAA GGGAAAAGCA
    TCAGAGAAGC TTTCTGAAAA CAGTAAAATA TTAATTTCCA TGGCTAAGGA AAACATACCA
    CCTAACAGTC AACAGACAAG GCGTTCTTTA GGAATTGATT ATGGATTAAG TTTACCACTT
    GGGGAAGAGT ATGAACAGAA GAAACATAAA CTGAAAGAAG AATTGCGACA AGATTACAGA
    CGCTATCTTA CTCAGGGTAT TACACAAGCA AAAAGAAAGA AAAATTTTCT ATCCACGAGT
    GAAACAGATC CATCTACTCT GGGAGTTTCC CTTCCTATTG GTGAGAGGTT ATCTGCTAAG
    GAAAGGTTGA AACTTGAACG TAACAAAGAA TACAATCAAT TTCTCAGGGA TAAGGAAGAA
    TCCACTGAAA AGTTCAGACA GGTAGAAAAG AGTACTGAGC AGAAGAATCA AAGAAGTAAA
    AAACCTATCA GTCAGGTTAA ACCTGATTTA CCTTCACAAA TACAATCATC TTTCGAAAAT
    TCAGAGGGTC CTAGAAAAGA TGTCTTAACT CCTTCAGAGG CATATGAAGA ACTTCTAAAC
    CAAAGACGAC TAGAAGAGGA CAGGTACCGA AAATTAGATG ATGAAATTGA ATTAAGGAAC
    AATAGAAGAG TCATTAAAAA GACTGATGAA GAAGTGGGCG TTTCCAGTAA AAAACATCAA
    AGGTTTGACA GCACGACTGA CATTCCAGAT AGAAGATTTC ACAGGTTTAA TGAGGATCTT
    GCTTTTGATA AACAGTCTTA CAGACCAGGC TATGATCCAG AAGTAAGTGA AGACATGGAT
    GAGAGGTTTA GATATGAAAG TGATTTTGAT AGAAGACTTT TGAGAGTTTA TACAAAAAAC
    AGGATGTACG GGAACAGACG AGGCAATGAG CCTCCTATGG AGTGTGATGG TGATGTCACA
    GAACAGTCAA ACATACGAAT TTCATCTGCT GGAAATCAAA GTGCTCGAGA CAATGAACCA
    TCCAAACCTA CTTATCGAGA GACATGCAGT CCTTTTGCGG GAATGCTCTT TGGGGGTGAA
    GATCGAGAAC TTATTCAGAG AAGGAAAGAG AAATATAGAC AAGAACTATT GGAACAAATG
    GCTGAACAAC AGAGGAACAA GAGACGAGAA AAAGATTTGG ATCTCAGGGT TGCAGCTTCT
    GGAGCACAAG ACCCTGAGAA ATCGCCTGAT AGACTAAAGC AGTTTAGTGT GGCACCAAGA
    CACTTTGAAG AGATGATACC ACCTGAGAGA CCCAGAATAG CTTTCCAGAC ACCTCTCCCT
    CCTCTGTCCG CCCCATCTGT CCCACCCATC CCATCAGTTT ACTCCATCCC ATCTCAGAGT
    GAAGATGTGC ACAGTGGACT CAGCAGCACT CTTGGTGACA TGGTGCCTCC CAGAGTTGCA
    CCTCTGCCTC CACCTCCCAT GCTGCCCCCT TTGGCTGCGA ACTACCGAAC TCCTTACGAT
    GACGCGTACT ATTTCTATGG GGCCAGGAAT ACTTTGGATC CTAGTCTTGC TTATTATGGT
    TCAGGGATGA TGGGAGTACA GCCTGCTCCT GCTCCTTATG TTACAGCTCC TGTCACCCAC
    CAGCCAGTAC AGCCTATTGT GAGTACAGTT GGACAGAATG AGCTGAAAAT TACAAGTAAT
    CGAGCAATAA ATTCAGGATT GTTTTTTGAA GATAAACCAA AGCCTTCAAA ACAGTCATTT
    CAGTCTTACC AGGAAGCTTT GCAGCAGCAG ATTCAAGAAA GACAAGAAAG AAGAAAGAAA
    GAACGTGAAG AAAAAGAAGT ATATGAAGCC AAATTAGAAG CTGAAATGAG AAGTTACAAC
    CCCTGGGGGA AAGGTGGAGG TGGTGCTCCT CTGAGAGATG ACAAAGGAAA TCTGATAACT
    GATTTGAATA GGATGCACAG ACAAAATATA GATGCCTACC ATAACCCAGA TGCAAGAGCA
    TATGAAGATA AAAGGGCTGT TGTATCTCTA GACCAAAATT TAGCCACTTC AAATGCGGAG
    AACCAAGAAG ATGCTGCAAA TAAAAACTCA GGTCATATGC AATCACAGAG CTCTCCTTTT
    GCTCGGGGAA ATATTTTTAG TGAGCCTCCA ACTGAACTTC AAATTAAACA GCAGGAATTA
    TACAAGAATT TTCTTCGTTT TCAGATTGAG GAAAAGAAGC AAAGAGAAGA AGCAGAGCGA
    GAGAGATTAA GAATTGCTGA AGAAAAAGAA GAAAAACGGC TTGCAGAACA GAGAGCTCGA
    ATCCAGCAAG AGTATGAAGA GGAGCAGGAA AAGAAAAGGG AGAAAGAGGA GGAGCAAAGA
    CTGAAAAACG AAGAGCTTAT TCGGTTAGCT GAAGAAAGAC GAAAAGAAGC AGAAAGAAAG
    AAGAAAGAAG AAGAAGAAAA ACATAATCTA CAACTTCAGC ACTACTATGA AAGAGAAAAC
    ATGATTGGGG AAGATACAAA GCACTTGAGA CAGCCTTCTC CTGTGGTTCC TGCTCTTCAG
    AATAAGATTG CAAGCAGACT CCAAAGACCT CCTTCAGTTG ACAGCATTAT AACTTCCTTT
    ACTCAGGACA GTTCCATGTC CATGGCACAA TCTCCTCCGG TACCTGCCAG GAAAAACCAG
    CTTCGTGCAG AAGAGGAGAA AAAAAATGTA ATTATGGAAT TATCAGAAAT GAGAAAACAG
    CTTCGTAGTG AAGAGCGACG TCTGCAGGGG CGGTTACTAC ACATTGATAG TGACGACGAC
    ATTCATATAA GGAAAAGAGA AAGTTCCATG GATGTATTTG ATATGGCCAG ACATCGATTA
    CAAGCTCCTG TCAGAAGACC GTCACCTAAG AGCTTAGACT CTGCTTCTTC TCAGAATATT
    CATGACTTTA AGGAGCTGAA AGATAGAGAT TCAGAAACGC GAGCTGATCT GAAATCTATG
    TACCCAGATG ATCCAAGAGA TAATGACACC TTGGAGATAG AACAACAAGC CTTGCTAAGA
    GAGCAGCAGA AGAGACTGAA TAAACTAAAA ATGCAGGAAG TTGACTTGGA TGCTGTCCCA
    AGCACTAAAG TACGGGACCA CAGAATGCCC AGAGAGGACA CTGATGATTT CTTGAAAAAC
    TCATTATTAG AATCTGATAG TGCTTTTATT GGTGCGCATG GCGAGACGTA CCCAGCCATT
    GAGGATGGGG GCTTCCTGCT GCTATCTCCG CTGCCCTCCG CCAGAGAGCG CAGAAGGAAC
    AAACTGAAAG GATTGGACTT TGATAACAGT CACCCTGATG TGCCACCAGA TGGTGTCTCT
    TTAAAATCTG TATCCAGTAT AAATATTGAT CAGCTTAGAA TGAGAAATGA AGAACGAATG
    CGAAGACTGA ATGAACTTCA GAATAAACCT ATTACTACAG ATGATGAGAG TTCACTGGTG
    GACCCTGATG ACATCATGAG GCACCGGGGT GACGACGGAT CAAACTCTGT AGCCACTGAG
    CCCTGGCTCC GCCCAGGCAC CTCAGAAACA CTGAAGCGAT TCATGGTGGA ACAGCTGAGG
    CAGGAGCAGC AGCAGGGTCC TGGCAAACCA GGCGCTTTCA CCTGGCAGGG ACTGTCTACT
    GCACACGGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857359.1
    CDS273..4184
    Translation

    Target ORF information:

    RefSeq Version XM_025001591.1
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGCCTC CGGGGCGGCA GGGGGCGCCC CCGGCCGCGA GCCTCTGCCC AGCGGCCGCA 
    GTGGGGCGGG GCCACGCCCG GCCGTTGGCG AAGGGCGGGC GGAGGGCTCG GCCTGAGAGG
    CGGAGCCCGG GTGGCTTGTC ACACCGTTCC CGCGCTGGGC GACTGCCGTC GCTTCTTCGG
    ACCGCGGCTA AGAGCCTCGC TAGAACGCTG TGCTGTTCCC CGACGCCAGA CCGCTTAGCT
    GATATCTTCA AAATGGCTGA TAATTTGGAT GAATTTATTG AAGAGCGAAA AGTCAGATTG
    GCCAGAGACA AAGCAGAATT GGAAAGTGAT CCACCGTACA TGGAAATGAA GGGAAAAGCA
    TCAGAGAAGC TTTCTGAAAA CAGTAAAATA TTAATTTCCA TGGCTAAGGA AAACATACCA
    CCTAACAGTC AACAGACAAG GCGTTCTTTA GGAATTGATT ATGGATTAAG TTTACCACTT
    GGGGAAGAGT ATGAACAGAA GAAACATAAA CTGAAAGAAG AATTGCGACA AGATTACAGA
    CGCTATCTTA CTCAGGGTAT TACACAAGCA AAAAGAAAGA AAAATTTTCT ATCCACGAGT
    GAAACAGATC CATCTACTCT GGGAGTTTCC CTTCCTATTG GTGAGAGGTT ATCTGCTAAG
    GAAAGGTTGA AACTTGAACG TAACAAAGAA TACAATCAAT TTCTCAGGGA TAAGGAAGAA
    TCCACTGAAA AGTTCAGACA GGTAGAAAAG AGTACTGAGC AGAAGAATCA AAGAAGTAAA
    AAACCTATCA GTCAGGTTAA ACCTGATTTA CCTTCACAAA TACAATCATC TTTCGAAAAT
    TCAGAGGGTC CTAGAAAAGA TGTCTTAACT CCTTCAGAGG CATATGAAGA ACTTCTAAAC
    CAAAGACGAC TAGAAGAGGA CAGGTACCGA AAATTAGATG ATGAAATTGA ATTAAGGAAC
    AATAGAAGAG TCATTAAAAA GACTGATGAA GAAGTGGGCG TTTCCAGTAA AAAACATCAA
    AGGTTTGACA GCACGACTGA CATTCCAGAT AGAAGATTTC ACAGGTTTAA TGAGGATCTT
    GCTTTTGATA AACAGTCTTA CAGACCAGGC TATGATCCAG AAGTAAGTGA AGACATGGAT
    GAGAGGTTTA GATATGAAAG TGATTTTGAT AGAAGACTTT TGAGAGTTTA TACAAAAAAC
    AGGATGTACG GGAACAGACG AGGCAATGAG CCTCCTATGG AGTGTGATGG TGATGTCACA
    GAACAGTCAA ACATACGAAT TTCATCTGCT GGAAATCAAA GTGCTCGAGA CAATGAACCA
    TCCAAACCTA CTTATCGAGA GACATGCAGT CCTTTTGCGG GAATGCTCTT TGGGGGTGAA
    GATCGAGAAC TTATTCAGAG AAGGAAAGAG AAATATAGAC AAGAACTATT GGAACAAATG
    GCTGAACAAC AGAGGAACAA GAGACGAGAA AAAGATTTGG ATCTCAGGGT TGCAGCTTCT
    GGAGCACAAG ACCCTGAGAA ATCGCCTGAT AGACTAAAGC AGTTTAGTGT GGCACCAAGA
    CACTTTGAAG AGATGATACC ACCTGAGAGA CCCAGAATAG CTTTCCAGAC ACCTCTCCCT
    CCTCTGTCCG CCCCATCTGT CCCACCCATC CCATCAGTTT ACTCCATCCC ATCTCAGAGT
    GAAGATGTGC ACAGTGGACT CAGCAGCACT CTTGGTGACA TGGTGCCTCC CAGAGTTGCA
    CCTCTGCCTC CACCTCCCAT GCTGCCCCCT TTGGCTGCGA ACTACCGAAC TCCTTACGAT
    GACGCGTACT ATTTCTATGG GGCCAGGAAT ACTTTGGATC CTAGTCTTGC TTATTATGGT
    TCAGGGATGA TGGGAGTACA GCCTGCTCCT GCTCCTTATG TTACAGCTCC TGTCACCCAC
    CAGCCAGTAC AGCCTATTGT GAGTACAGTT GGACAGAATG AGCTGAAAAT TACAAGTAAT
    CGAGCAATAA ATTCAGGATT GTTTTTTGAA GATAAACCAA AGCCTTCAAA ACAGTCATTT
    CAGTCTTACC AGGAAGCTTT GCAGCAGCAG ATTCAAGAAA GACAAGAAAG AAGAAAGAAA
    GAACGTGAAG AAAAAGAAGT ATATGAAGCC AAATTAGAAG CTGAAATGAG AAGTTACAAC
    CCCTGGGGGA AAGGTGGAGG TGGTGCTCCT CTGAGAGATG ACAAAGGAAA TCTGATAACT
    GATTTGAATA GGATGCACAG ACAAAATATA GATGCCTACC ATAACCCAGA TGCAAGAGCA
    TATGAAGATA AAAGGGCTGT TGTATCTCTA GACCAAAATT TAGCCACTTC AAATGCGGAG
    AACCAAGAAG ATGCTGCAAA TAAAAACTCA GGTCATATGC AATCACAGAG CTCTCCTTTT
    GCTCGGGGAA ATATTTTTAG TGAGCCTCCA ACTGAACTTC AAATTAAACA GCAGGAATTA
    TACAAGAATT TTCTTCGTTT TCAGATTGAG GAAAAGAAGC AAAGAGAAGA AGCAGAGCGA
    GAGAGATTAA GAATTGCTGA AGAAAAAGAA GAAAAACGGC TTGCAGAACA GAGAGCTCGA
    ATCCAGCAAG AGTATGAAGA GGAGCAGGAA AAGAAAAGGG AGAAAGAGGA GGAGCAAAGA
    CTGAAAAACG AAGAGCTTAT TCGGTTAGCT GAAGAAAGAC GAAAAGAAGC AGAAAGAAAG
    AAGAAAGAAG AAGAAGAAAA ACATAATCTA CAACTTCAGC ACTACTATGA AAGAGAAAAC
    ATGATTGGGG AAGATACAAA GCACTTGAGA CAGCCTTCTC CTGTGGTTCC TGCTCTTCAG
    AATAAGATTG CAAGCAGACT CCAAAGACCT CCTTCAGTTG ACAGCATTAT AACTTCCTTT
    ACTCAGGACA GTTCCATGTC CATGGCACAA TCTCCTCCGG TACCTGCCAG GAAAAACCAG
    CTTCGTGCAG AAGAGGAGAA AAAAAATGTA ATTATGGAAT TATCAGAAAT GAGAAAACAG
    CTTCGTAGTG AAGAGCGACG TCTGCAGGGG CGGTTACTAC ACATTGATAG TGACGACGAC
    ATTCATATAA GGAAAAGAGA AAGTTCCATG GATGTATTTG ATATGGCCAG ACATCGATTA
    CAAGCTCCTG TCAGAAGACC GTCACCTAAG AGCTTAGACT CTGCTTCTTC TCAGAATATT
    CATGACTTTA AGGAGCTGAA AGATAGAGAT TCAGAAACGC GAGCTGATCT GAAATCTATG
    TACCCAGATG ATCCAAGAGA TAATGACACC TTGGAGATAG AACAACAAGC CTTGCTAAGA
    GAGCAGCAGA AGAGACTGAA TAAACTAAAA ATGCAGGAAG TTGACTTGGA TGCTGTCCCA
    AGCACTAAAG TACGGGACCA CAGAATGCCC AGAGAGGACA CTGATGATTT CTTGAAAAAC
    TCATTATTAG AATCTGATAG TGCTTTTATT GGTGCGCATG GCGAGACGTA CCCAGCCATT
    GAGGATGGGG GCTTCCTGCT GCTATCTCCG CTGCCCTCCG CCAGAGAGCG CAGAAGGAAC
    AAACTGAAAG GATTGGACTT TGATAACAGT CACCCTGATG TGCCACCAGA TGGTGTCTCT
    TTAAAATCTG TATCCAGTAT AAATATTGAT CAGCTTAGAA TGAGAAATGA AGAACGAATG
    CGAAGACTGA ATGAACTTCA GAATAAACCT ATTACTACAG ATGATGAGAG TTCACTGGTG
    GACCCTGATG ACATCATGAG GCACCGGGGT GACGACGGAT CAAACTCTGT AGCCACTGAG
    CCCTGGCTCC GCCCAGGCAC CTCAGAAACA CTGAAGCGAT TCATGGTGGA ACAGCTGAGG
    CAGGAGCAGC AGCAGGGTCC TGGCAAACCA GGCGCTTTCA CCTGGCAGGG ACTGTCTACT
    GCACACGGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222315
    Clone ID Related Accession (Same CDS sequence) XM_025001592.1
    Accession Version XM_025001592.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGCAGAAGA ATCAAAGAAG TAAAAAACCT ATCAGTCAGG TTAAACCTGA TTTACCTTCA
    CAAATACAAT CATCTTTCGA AAATTCAGAG GGTCCTAGAA AAGATGTCTT AACTCCTTCA
    GAGGCATATG AAGAACTTCT AAACCAAAGA CGACTAGAAG AGGACAGGTA CCGAAAATTA
    GATGATGAAA TTGAATTAAG GAACAATAGA AGAGTCATTA AAAAGACTGA TGAAGAAGTG
    GGCGTTTCCA GTAAAAAACA TCAAAGGTTT GACAGCACGA CTGACATTCC AGATAGAAGA
    TTTCACAGGT TTAATGAGGA TCTTGCTTTT GATAAACAGT CTTACAGACC AGGCTATGAT
    CCAGAAGTAA GTGAAGACAT GGATGAGAGG TTTAGATATG AAAGTGATTT TGATAGAAGA
    CTTTTGAGAG TTTATACAAA AAACAGAGTT GCACCTCTGC CTCCACCTCC CATGCTGCCC
    CCTTTGGCTG CGAACTACCG AACTCCTTAC GATGACGCGT ACTATTTCTA TGGGGCCAGG
    AATACTTTGG ATCCTAGTCT TGCTTATTAT GGTTCAGGGA TGATGGGAGT ACAGCCTGCT
    CCTGCTCCTT ATGTTACAGC TCCTGTCACC CACCAGCCAG TACAGCCTAT TGTGAGTACA
    GTTGGACAGA ATGAGCTGAA AATTACAAGT AATCGAGCAA TAAATTCAGG ATTGTTTTTT
    GAAGATAAAC CAAAGCCTTC AAAACAGTCA TTTCAGTCTT ACCAGGAAGC TTTGCAGCAG
    CAGATTCAAG AAAGACAAGA AAGAAGAAAG AAAGAACGTG AAGAAAAAGA AGTATATGAA
    GCCAAATTAG AAGCTGAAAT GAGAAGTTAC AACCCCTGGG GGAAAGGTGG AGGTGGTGCT
    CCTCTGAGAG ATGACAAAGG AAATCTGATA ACTGATTTGA ATAGGATGCA CAGACAAAAT
    ATAGATGCCT ACCATAACCC AGATGCAAGA GCATATGAAG ATAAAAGGGC TGTTGTATCT
    CTAGACCAAA ATTTAGCCAC TTCAAATGCG GAGAACCAAG AAGATGCTGC AAATAAAAAC
    TCAGGTCATA TGCAATCACA GAGCTCTCCT TTTGCTCGGG GAAATATTTT TAGTGAGCCT
    CCAACTGAAC TTCAAATTAA ACAGCAGGAA TTATACAAGA ATTTTCTTCG TTTTCAGATT
    GAGGAAAAGA AGCAAAGAGA AGAAGCAGAG CGAGAGAGAT TAAGAATTGC TGAAGAAAAA
    GAAGAAAAAC GGCTTGCAGA ACAGAGAGCT CGAATCCAGC AAGAGTATGA AGAGGAGCAG
    GAAAAGAAAA GGGAGAAAGA GGAGGAGCAA AGACTGAAAA ACGAAGAGCT TATTCGGTTA
    GCTGAAGAAA GACGAAAAGA AGCAGAAAGA AAGAAGAAAG AAGAAGAAGA AAAACATAAT
    CTACAACTTC AGCACTACTA TGAAAGAGAA AACATGATTG GGGAAGATAC AAAGCACTTG
    AGACAGCCTT CTCCTGTGGT TCCTGCTCTT CAGAATAAGA TTGCAAGCAG ACTCCAAAGA
    CCTCCTTCAG TTGACAGCAT TATAACTTCC TTTACTCAGG ACAGTTCCAT GTCCATGGCA
    CAATCTCCTC CGGTACCTGC CAGGAAAAAC CAGCTTCGTG CAGAAGAGGA GAAAAAAAAT
    GTAATTATGG AATTATCAGA AATGAGAAAA CAGCTTCGTA GTGAAGAGCG ACGTCTGCAG
    GGGCGGTTAC TACACATTGA TAGTGACGAC GACATTCATA TAAGGAAAAG AGAAAGTTCC
    ATGGATGTAT TTGATATGGC CAGACATCGA TTACAAGCTC CTGTCAGAAG ACCGTCACCT
    AAGAGCTTAG ACTCTGCTTC TTCTCAGAAT ATTCATGACT TTAAGGAGCT GAAAGATAGA
    GATTCAGAAA CGCGAGCTGA TCTGAAATCT ATGTACCCAG ATGATCCAAG AGATAATGAC
    ACCTTGGAGA TAGAACAACA AGCCTTGCTA AGAGAGCAGC AGAAGAGACT GAATAAACTA
    AAAATGCAGG AAGTTGACTT GGATGCTGTC CCAAGCACTA AAGTACGGGA CCACAGAATG
    CCCAGAGAGG ACACTGATGA TTTCTTGAAA AACTCATTAT TAGAATCTGA TAGTGCTTTT
    ATTGGTGCGC ATGGCGAGAC GTACCCAGCC ATTGAGGATG GGGGCTTCCT GCTGCTATCT
    CCGCTGCCCT CCGCCAGAGA GCGCAGAAGG AACAAACTGA AAGGATTGGA CTTTGATAAC
    AGTCACCCTG ATGTGCCACC AGATGGTGTC TCTTTAAAAT CTGTATCCAG TATAAATATT
    GATCAGCTTA GAATGAGAAA TGAAGAACGA ATGCGAAGAC TGAATGAACT TCAGAATAAA
    CCTATTACTA CAGATGATGA GAGTTCACTG GTGGACCCTG ATGACATCAT GAGGCACCGG
    GGTGACGACG GATCAAACTC TGTAGCCACT GAGCCCTGGC TCCGCCCAGG CACCTCAGAA
    ACACTGAAGC GATTCATGGT GGAACAGCTG AGGCAGGAGC AGCAGCAGGG TCCTGGCAAA
    CCAGGCGCTT TCACCTGGCA GGGACTGTCT ACTGCACACG GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857360.1
    CDS181..3285
    Translation

    Target ORF information:

    RefSeq Version XM_025001592.1
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGCAGAAGA ATCAAAGAAG TAAAAAACCT ATCAGTCAGG TTAAACCTGA TTTACCTTCA
    CAAATACAAT CATCTTTCGA AAATTCAGAG GGTCCTAGAA AAGATGTCTT AACTCCTTCA
    GAGGCATATG AAGAACTTCT AAACCAAAGA CGACTAGAAG AGGACAGGTA CCGAAAATTA
    GATGATGAAA TTGAATTAAG GAACAATAGA AGAGTCATTA AAAAGACTGA TGAAGAAGTG
    GGCGTTTCCA GTAAAAAACA TCAAAGGTTT GACAGCACGA CTGACATTCC AGATAGAAGA
    TTTCACAGGT TTAATGAGGA TCTTGCTTTT GATAAACAGT CTTACAGACC AGGCTATGAT
    CCAGAAGTAA GTGAAGACAT GGATGAGAGG TTTAGATATG AAAGTGATTT TGATAGAAGA
    CTTTTGAGAG TTTATACAAA AAACAGAGTT GCACCTCTGC CTCCACCTCC CATGCTGCCC
    CCTTTGGCTG CGAACTACCG AACTCCTTAC GATGACGCGT ACTATTTCTA TGGGGCCAGG
    AATACTTTGG ATCCTAGTCT TGCTTATTAT GGTTCAGGGA TGATGGGAGT ACAGCCTGCT
    CCTGCTCCTT ATGTTACAGC TCCTGTCACC CACCAGCCAG TACAGCCTAT TGTGAGTACA
    GTTGGACAGA ATGAGCTGAA AATTACAAGT AATCGAGCAA TAAATTCAGG ATTGTTTTTT
    GAAGATAAAC CAAAGCCTTC AAAACAGTCA TTTCAGTCTT ACCAGGAAGC TTTGCAGCAG
    CAGATTCAAG AAAGACAAGA AAGAAGAAAG AAAGAACGTG AAGAAAAAGA AGTATATGAA
    GCCAAATTAG AAGCTGAAAT GAGAAGTTAC AACCCCTGGG GGAAAGGTGG AGGTGGTGCT
    CCTCTGAGAG ATGACAAAGG AAATCTGATA ACTGATTTGA ATAGGATGCA CAGACAAAAT
    ATAGATGCCT ACCATAACCC AGATGCAAGA GCATATGAAG ATAAAAGGGC TGTTGTATCT
    CTAGACCAAA ATTTAGCCAC TTCAAATGCG GAGAACCAAG AAGATGCTGC AAATAAAAAC
    TCAGGTCATA TGCAATCACA GAGCTCTCCT TTTGCTCGGG GAAATATTTT TAGTGAGCCT
    CCAACTGAAC TTCAAATTAA ACAGCAGGAA TTATACAAGA ATTTTCTTCG TTTTCAGATT
    GAGGAAAAGA AGCAAAGAGA AGAAGCAGAG CGAGAGAGAT TAAGAATTGC TGAAGAAAAA
    GAAGAAAAAC GGCTTGCAGA ACAGAGAGCT CGAATCCAGC AAGAGTATGA AGAGGAGCAG
    GAAAAGAAAA GGGAGAAAGA GGAGGAGCAA AGACTGAAAA ACGAAGAGCT TATTCGGTTA
    GCTGAAGAAA GACGAAAAGA AGCAGAAAGA AAGAAGAAAG AAGAAGAAGA AAAACATAAT
    CTACAACTTC AGCACTACTA TGAAAGAGAA AACATGATTG GGGAAGATAC AAAGCACTTG
    AGACAGCCTT CTCCTGTGGT TCCTGCTCTT CAGAATAAGA TTGCAAGCAG ACTCCAAAGA
    CCTCCTTCAG TTGACAGCAT TATAACTTCC TTTACTCAGG ACAGTTCCAT GTCCATGGCA
    CAATCTCCTC CGGTACCTGC CAGGAAAAAC CAGCTTCGTG CAGAAGAGGA GAAAAAAAAT
    GTAATTATGG AATTATCAGA AATGAGAAAA CAGCTTCGTA GTGAAGAGCG ACGTCTGCAG
    GGGCGGTTAC TACACATTGA TAGTGACGAC GACATTCATA TAAGGAAAAG AGAAAGTTCC
    ATGGATGTAT TTGATATGGC CAGACATCGA TTACAAGCTC CTGTCAGAAG ACCGTCACCT
    AAGAGCTTAG ACTCTGCTTC TTCTCAGAAT ATTCATGACT TTAAGGAGCT GAAAGATAGA
    GATTCAGAAA CGCGAGCTGA TCTGAAATCT ATGTACCCAG ATGATCCAAG AGATAATGAC
    ACCTTGGAGA TAGAACAACA AGCCTTGCTA AGAGAGCAGC AGAAGAGACT GAATAAACTA
    AAAATGCAGG AAGTTGACTT GGATGCTGTC CCAAGCACTA AAGTACGGGA CCACAGAATG
    CCCAGAGAGG ACACTGATGA TTTCTTGAAA AACTCATTAT TAGAATCTGA TAGTGCTTTT
    ATTGGTGCGC ATGGCGAGAC GTACCCAGCC ATTGAGGATG GGGGCTTCCT GCTGCTATCT
    CCGCTGCCCT CCGCCAGAGA GCGCAGAAGG AACAAACTGA AAGGATTGGA CTTTGATAAC
    AGTCACCCTG ATGTGCCACC AGATGGTGTC TCTTTAAAAT CTGTATCCAG TATAAATATT
    GATCAGCTTA GAATGAGAAA TGAAGAACGA ATGCGAAGAC TGAATGAACT TCAGAATAAA
    CCTATTACTA CAGATGATGA GAGTTCACTG GTGGACCCTG ATGACATCAT GAGGCACCGG
    GGTGACGACG GATCAAACTC TGTAGCCACT GAGCCCTGGC TCCGCCCAGG CACCTCAGAA
    ACACTGAAGC GATTCATGGT GGAACAGCTG AGGCAGGAGC AGCAGCAGGG TCCTGGCAAA
    CCAGGCGCTT TCACCTGGCA GGGACTGTCT ACTGCACACG GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222316
    Clone ID Related Accession (Same CDS sequence) XM_025001593.1
    Accession Version XM_025001593.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCGGCCTC CGGGGCGGCA GGGGGCGCCC CCGGCCGCGA GCCTCTGCCC AGCGGCCGCA 
    GTGGGGCGGG GCCACGCCCG GCCGTTGGCG AAGGGCGGGC GGAGGGCTCG GCCTGAGAGG
    CGGAGCCCGG GTGGCTTGTC ACACCGTTCC CGCGCTGGGC GACTGCCGTC GCTTCTTCGG
    ACCGCGGCTA AGAGCCTCGC TAGAACGCTG TGCTGTTCCC CGACGCCAGA CCGCTTAGCT
    GATATCTTCA AAATGGCTGA TAATTTGGAT GAATTTATTG AAGAGCGAAA AGTCAGATTG
    GCCAGAGACA AAGCAGAATT GGAAAGTGAT CCACCGTACA TGGAAATGAA GGGAAAAGCA
    TCAGAGAAGC TTTCTGAAAA CAGTAAAATA TTAATTTCCA TGGCTAAGGA AAACATACCA
    CCTAACAGTC AACAGACAAG GCGTTCTTTA GGAATTGATT ATGGATTAAG TTTACCACTT
    GGGGAAGAGT ATGAACAGAA GAAACATAAA CTGAAAGAAG AATTGCGACA AGATTACAGA
    CGCTATCTTA CTCAGAAAAA TTTTCTATCC ACGAGTGAAA CAGATCCATC TACTCTGGGA
    GTTTCCCTTC CTATTGGTGA GAGGTTATCT GCTAAGGAAA GGTTGAAACT TGAACGTAAC
    AAAGAATACA ATCAATTTCT CAGGGATAAG GAAGAATCCA CTGAAAAGTT CAGACAGGTA
    GAAAAGAGTA CTGAGAAGAA TCAAAGAAGT AAAAAACCTA TCAGTCAGGT TAAACCTGAT
    TTACCTTCAC AAATACAATC ATCTTTCGAA AATTCAGAGG GTCCTAGAAA AGATGTCTTA
    ACTCCTTCAG AGGCATATGA AGAACTTCTA AACCAAAGAC GACTAGAAGA GGACAGGTAC
    CGAAAATTAG ATGATGAAAT TGAATTAAGG AACAATAGAA GAGTCATTAA AAAGACTGAT
    GAAGAAGTGG GCGTTTCCAG TAAAAAACAT CAAAGGTTTG ACAGCACGAC TGACATTCCA
    GATAGAAGAT TTCACAGGTT TAATGAGGAT CTTGCTTTTG ATAAACAGTC TTACAGACCA
    GGCTATGATC CAGAAGTAAG TGAAGACATG GATGAGAGGT TTAGATATGA AAGTGATTTT
    GATAGAAGAC TTTTGAGAGT TTATACAAAA AACAGAGTTG CACCTCTGCC TCCACCTCCC
    ATGCTGCCCC CTTTGGCTGC GAACTACCGA ACTCCTTACG ATGACGCGTA CTATTTCTAT
    GGGGCCAGGA ATACTTTGGA TCCTAGTCTT GCTTATTATG GTTCAGGGAT GATGGGAGTA
    CAGCCTGCTC CTGCTCCTTA TGTTACAGCT CCTGTCACCC ACCAGCCAGT ACAGCCTATT
    GTGAGTACAG TTGGACAGAA TGAGCTGAAA ATTACAAGTA ATCGAGCAAT AAATTCAGGA
    TTGTTTTTTG AAGATAAACC AAAGCCTTCA AAACAGTCAT TTCAGTCTTA CCAGGAAGCT
    TTGCAGCAGC AGATTCAAGA AAGACAAGAA AGAAGAAAGA AAGAACGTGA AGAAAAAGAA
    GTATATGAAG CCAAATTAGA AGCTGAAATG AGAAGTTACA ACCCCTGGGG GAAAGGTGGA
    GGTGGTGCTC CTCTGAGAGA TGACAAAGGA AATCTGATAA CTGATTTGAA TAGGATGCAC
    AGACAAAATA TAGATGCCTA CCATAACCCA GATGCAAGAG CATATGAAGA TAAAAGGGCT
    GTTGTATCTC TAGACCAAAA TTTAGCCACT TCAAATGCGG AGAACCAAGA AGATGCTGCA
    AATAAAAACT CAGGTCATAT GCAATCACAG AGCTCTCCTT TTGCTCGGGG AAATATTTTT
    AGTGAGCCTC CAACTGAACT TCAAATTAAA CAGCAGGAAT TATACAAGAA TTTTCTTCGT
    TTTCAGATTG AGGAAAAGAA GCAAAGAGAA GAAGCAGAGC GAGAGAGATT AAGAATTGCT
    GAAGAAAAAG AAGAAAAACG GCTTGCAGAA CAGAGAGCTC GAATCCAGCA AGAGTATGAA
    GAGGAGCAGG AAAAGAAAAG GGAGAAAGAG GAGGAGCAAA GACTGAAAAA CGAAGAGCTT
    ATTCGGTTAG CTGAAGAAAG ACGAAAAGAA GCAGAAAGAA AGAAGAAAGA AGAAGAAGAA
    AAACATAATC TACAACTTCA GCACTACTAT GAAAGAGAAA ACATGATTGG GGAAGATACA
    AAGCACTTGA GACAGCCTTC TCCTGTGGTT CCTGCTCTTC AGAATAAGAT TGCAAGCAGA
    CTCCAAAGAC CTCCTTCAGT TGACAGCATT ATAACTTCCT TTACTCAGGA CAGTTCCATG
    TCCATGGCAC AATCTCCTCC GGTACCTGCC AGGAAAAACC AGCTTCGTGC AGAAGAGGAG
    AAAAAAAATG TAATTATGGA ATTATCAGAA ATGAGAAAAC AGCTTCGTAG TGAAGAGCGA
    CGTCTGCAGG GGCGGTTACT ACACATTGAT AGTGACGACG ACATTCATAT AAGGAAAAGA
    GAAAGTTCCA TGGATGTATT TGATATGGCC AGACATCGAT TACAAGCTCC TGTCAGAAGA
    CCGTCACCTA AGAGCTTAGA CTCTGCTTCT TCTCAGAATA TTCATGACTT TAAGGAGCTG
    AAAGATAGAG ATTCAGAAAC GCGAGCTGAT CTGAAATCTA TGTACCCAGA TGATCCAAGA
    GATAATGACA CCTTGGAGAT AGAACAACAA GCCTTGCTAA GAGAGCAGCA GAAGAGACTG
    AATAAACTAA AAATGCAGGA AGTTGACTTG GATGCTGTCC CAAGCACTAA AGTACGGGAC
    CACAGAATGC CCAGAGAGGA CACTGATGAT TTCTTGAAAA ACTCATTATT AGAATCTGAT
    AGTGCTTTTA TTGGTGCGCA TGGCGAGACG TACCCAGCCA TTGAGGATGG GGGCTTCCTG
    CTGCTATCTC CGCTGCCCTC CGCCAGAGAG CGCAGAAGGA ACAAACTGAA AGGATTGGAC
    TTTGATAACA GTCACCCTGA TGTGCCACCA GATGGTGTCT CTTTAAAATC TGTATCCAGT
    ATAAATATTG ATCAGCTTAG AATGAGAAAT GAAGAACGAA TGCGAAGACT GAATGAACTT
    CAGAATAAAC CTATTACTAC AGATGATGAG AGTTCACTGG TGGACCCTGA TGACATCATG
    AGGCACCGGG GTGACGACGG ATCAAACTCT GTAGCCACTG AGCCCTGGCT CCGCCCAGGC
    ACCTCAGAAA CACTGAAGCG ATTCATGGTG GAACAGCTGA GGCAGGAGCA GCAGCAGGGT
    CCTGGCAAAC CAGGCGCTTT CACCTGGCAG GGACTGTCTA CTGCACACGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857361.1
    CDS16..3369
    Translation

    Target ORF information:

    RefSeq Version XM_025001593.1
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001593.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCGGCCTC CGGGGCGGCA GGGGGCGCCC CCGGCCGCGA GCCTCTGCCC AGCGGCCGCA 
    GTGGGGCGGG GCCACGCCCG GCCGTTGGCG AAGGGCGGGC GGAGGGCTCG GCCTGAGAGG
    CGGAGCCCGG GTGGCTTGTC ACACCGTTCC CGCGCTGGGC GACTGCCGTC GCTTCTTCGG
    ACCGCGGCTA AGAGCCTCGC TAGAACGCTG TGCTGTTCCC CGACGCCAGA CCGCTTAGCT
    GATATCTTCA AAATGGCTGA TAATTTGGAT GAATTTATTG AAGAGCGAAA AGTCAGATTG
    GCCAGAGACA AAGCAGAATT GGAAAGTGAT CCACCGTACA TGGAAATGAA GGGAAAAGCA
    TCAGAGAAGC TTTCTGAAAA CAGTAAAATA TTAATTTCCA TGGCTAAGGA AAACATACCA
    CCTAACAGTC AACAGACAAG GCGTTCTTTA GGAATTGATT ATGGATTAAG TTTACCACTT
    GGGGAAGAGT ATGAACAGAA GAAACATAAA CTGAAAGAAG AATTGCGACA AGATTACAGA
    CGCTATCTTA CTCAGAAAAA TTTTCTATCC ACGAGTGAAA CAGATCCATC TACTCTGGGA
    GTTTCCCTTC CTATTGGTGA GAGGTTATCT GCTAAGGAAA GGTTGAAACT TGAACGTAAC
    AAAGAATACA ATCAATTTCT CAGGGATAAG GAAGAATCCA CTGAAAAGTT CAGACAGGTA
    GAAAAGAGTA CTGAGAAGAA TCAAAGAAGT AAAAAACCTA TCAGTCAGGT TAAACCTGAT
    TTACCTTCAC AAATACAATC ATCTTTCGAA AATTCAGAGG GTCCTAGAAA AGATGTCTTA
    ACTCCTTCAG AGGCATATGA AGAACTTCTA AACCAAAGAC GACTAGAAGA GGACAGGTAC
    CGAAAATTAG ATGATGAAAT TGAATTAAGG AACAATAGAA GAGTCATTAA AAAGACTGAT
    GAAGAAGTGG GCGTTTCCAG TAAAAAACAT CAAAGGTTTG ACAGCACGAC TGACATTCCA
    GATAGAAGAT TTCACAGGTT TAATGAGGAT CTTGCTTTTG ATAAACAGTC TTACAGACCA
    GGCTATGATC CAGAAGTAAG TGAAGACATG GATGAGAGGT TTAGATATGA AAGTGATTTT
    GATAGAAGAC TTTTGAGAGT TTATACAAAA AACAGAGTTG CACCTCTGCC TCCACCTCCC
    ATGCTGCCCC CTTTGGCTGC GAACTACCGA ACTCCTTACG ATGACGCGTA CTATTTCTAT
    GGGGCCAGGA ATACTTTGGA TCCTAGTCTT GCTTATTATG GTTCAGGGAT GATGGGAGTA
    CAGCCTGCTC CTGCTCCTTA TGTTACAGCT CCTGTCACCC ACCAGCCAGT ACAGCCTATT
    GTGAGTACAG TTGGACAGAA TGAGCTGAAA ATTACAAGTA ATCGAGCAAT AAATTCAGGA
    TTGTTTTTTG AAGATAAACC AAAGCCTTCA AAACAGTCAT TTCAGTCTTA CCAGGAAGCT
    TTGCAGCAGC AGATTCAAGA AAGACAAGAA AGAAGAAAGA AAGAACGTGA AGAAAAAGAA
    GTATATGAAG CCAAATTAGA AGCTGAAATG AGAAGTTACA ACCCCTGGGG GAAAGGTGGA
    GGTGGTGCTC CTCTGAGAGA TGACAAAGGA AATCTGATAA CTGATTTGAA TAGGATGCAC
    AGACAAAATA TAGATGCCTA CCATAACCCA GATGCAAGAG CATATGAAGA TAAAAGGGCT
    GTTGTATCTC TAGACCAAAA TTTAGCCACT TCAAATGCGG AGAACCAAGA AGATGCTGCA
    AATAAAAACT CAGGTCATAT GCAATCACAG AGCTCTCCTT TTGCTCGGGG AAATATTTTT
    AGTGAGCCTC CAACTGAACT TCAAATTAAA CAGCAGGAAT TATACAAGAA TTTTCTTCGT
    TTTCAGATTG AGGAAAAGAA GCAAAGAGAA GAAGCAGAGC GAGAGAGATT AAGAATTGCT
    GAAGAAAAAG AAGAAAAACG GCTTGCAGAA CAGAGAGCTC GAATCCAGCA AGAGTATGAA
    GAGGAGCAGG AAAAGAAAAG GGAGAAAGAG GAGGAGCAAA GACTGAAAAA CGAAGAGCTT
    ATTCGGTTAG CTGAAGAAAG ACGAAAAGAA GCAGAAAGAA AGAAGAAAGA AGAAGAAGAA
    AAACATAATC TACAACTTCA GCACTACTAT GAAAGAGAAA ACATGATTGG GGAAGATACA
    AAGCACTTGA GACAGCCTTC TCCTGTGGTT CCTGCTCTTC AGAATAAGAT TGCAAGCAGA
    CTCCAAAGAC CTCCTTCAGT TGACAGCATT ATAACTTCCT TTACTCAGGA CAGTTCCATG
    TCCATGGCAC AATCTCCTCC GGTACCTGCC AGGAAAAACC AGCTTCGTGC AGAAGAGGAG
    AAAAAAAATG TAATTATGGA ATTATCAGAA ATGAGAAAAC AGCTTCGTAG TGAAGAGCGA
    CGTCTGCAGG GGCGGTTACT ACACATTGAT AGTGACGACG ACATTCATAT AAGGAAAAGA
    GAAAGTTCCA TGGATGTATT TGATATGGCC AGACATCGAT TACAAGCTCC TGTCAGAAGA
    CCGTCACCTA AGAGCTTAGA CTCTGCTTCT TCTCAGAATA TTCATGACTT TAAGGAGCTG
    AAAGATAGAG ATTCAGAAAC GCGAGCTGAT CTGAAATCTA TGTACCCAGA TGATCCAAGA
    GATAATGACA CCTTGGAGAT AGAACAACAA GCCTTGCTAA GAGAGCAGCA GAAGAGACTG
    AATAAACTAA AAATGCAGGA AGTTGACTTG GATGCTGTCC CAAGCACTAA AGTACGGGAC
    CACAGAATGC CCAGAGAGGA CACTGATGAT TTCTTGAAAA ACTCATTATT AGAATCTGAT
    AGTGCTTTTA TTGGTGCGCA TGGCGAGACG TACCCAGCCA TTGAGGATGG GGGCTTCCTG
    CTGCTATCTC CGCTGCCCTC CGCCAGAGAG CGCAGAAGGA ACAAACTGAA AGGATTGGAC
    TTTGATAACA GTCACCCTGA TGTGCCACCA GATGGTGTCT CTTTAAAATC TGTATCCAGT
    ATAAATATTG ATCAGCTTAG AATGAGAAAT GAAGAACGAA TGCGAAGACT GAATGAACTT
    CAGAATAAAC CTATTACTAC AGATGATGAG AGTTCACTGG TGGACCCTGA TGACATCATG
    AGGCACCGGG GTGACGACGG ATCAAACTCT GTAGCCACTG AGCCCTGGCT CCGCCCAGGC
    ACCTCAGAAA CACTGAAGCG ATTCATGGTG GAACAGCTGA GGCAGGAGCA GCAGCAGGGT
    CCTGGCAAAC CAGGCGCTTT CACCTGGCAG GGACTGTCTA CTGCACACGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25976
    Clone ID Related Accession (Same CDS sequence) XM_025001590.1 , XM_005215485.3 , NM_001193015.2 , XM_005215485.4 , XM_005215486.2 , XM_005215486.3
    Accession Version NM_001193015.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000014.1. On Apr 21, 2011 this sequence version replaced NM_001193015.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145420, SAMN03145422 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179944.2
    CDS76..3735
    Translation

    Target ORF information:

    RefSeq Version NM_001193015.2
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25976
    Clone ID Related Accession (Same CDS sequence) XM_025001590.1 , XM_005215485.3 , NM_001193015.2 , XM_005215485.4 , XM_005215486.2 , XM_005215486.3
    Accession Version XM_025001590.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857358.1
    CDS30..3689
    Translation

    Target ORF information:

    RefSeq Version XM_025001590.1
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001590.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25976
    Clone ID Related Accession (Same CDS sequence) XM_025001590.1 , XM_005215485.3 , NM_001193015.2 , XM_005215485.4 , XM_005215486.2 , XM_005215486.3
    Accession Version XM_005215485.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215542.1
    CDS60..3719
    Translation

    Target ORF information:

    RefSeq Version XM_005215485.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215485.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25976
    Clone ID Related Accession (Same CDS sequence) XM_025001590.1 , XM_005215485.3 , NM_001193015.2 , XM_005215485.4 , XM_005215486.2 , XM_005215486.3
    Accession Version XM_005215485.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215542.1
    CDS65..3724
    Translation

    Target ORF information:

    RefSeq Version XM_005215485.4
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215485.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25976
    Clone ID Related Accession (Same CDS sequence) XM_025001590.1 , XM_005215485.3 , NM_001193015.2 , XM_005215485.4 , XM_005215486.2 , XM_005215486.3
    Accession Version XM_005215486.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215543.1
    CDS102..3761
    Translation

    Target ORF information:

    RefSeq Version XM_005215486.2
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215486.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25976
    Clone ID Related Accession (Same CDS sequence) XM_025001590.1 , XM_005215485.3 , NM_001193015.2 , XM_005215485.4 , XM_005215486.2 , XM_005215486.3
    Accession Version XM_005215486.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215486.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215543.1
    CDS144..3803
    Translation

    Target ORF information:

    RefSeq Version XM_005215486.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215486.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGATTC AGAAACGCGA GCTGATCTGA AATCTATGTA CCCAGATGAT
    CCAAGAGATA ATGACACCTT GGAGATAGAA CAACAAGCCT TGCTAAGAGA GCAGCAGAAG
    AGACTGAATA AACTAAAAAT GCAGGAAGTT GACTTGGATG CTGTCCCAAG CACTAAAGTA
    CGGGACCACA GAATGCCCAG AGAGGACACT GATGATTTCT TGAAAAACTC ATTATTAGAA
    TCTGATAGTG CTTTTATTGG TGCGCATGGC GAGACGTACC CAGCCATTGA GGATGGGGGC
    TTCCTGCTGC TATCTCCGCT GCCCTCCGCC AGAGAGCGCA GAAGGAACAA ACTGAAAGGA
    TTGGACTTTG ATAACAGTCA CCCTGATGTG CCACCAGATG GTGTCTCTTT AAAATCTGTA
    TCCAGTATAA ATATTGATCA GCTTAGAATG AGAAATGAAG AACGAATGCG AAGACTGAAT
    GAACTTCAGA ATAAACCTAT TACTACAGAT GATGAGAGTT CACTGGTGGA CCCTGATGAC
    ATCATGAGGC ACCGGGGTGA CGACGGATCA AACTCTGTAG CCACTGAGCC CTGGCTCCGC
    CCAGGCACCT CAGAAACACT GAAGCGATTC ATGGTGGAAC AGCTGAGGCA GGAGCAGCAG
    CAGGGTCCTG GCAAACCAGG CGCTTTCACC TGGCAGGGAC TGTCTACTGC ACACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50841
    Clone ID Related Accession (Same CDS sequence) XM_005215479.4 , XM_005215479.3
    Accession Version XM_005215479.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215479.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGAAG AATCAAAGAA GTAAAAAACC TATCAGTCAG
    GTTAAACCTG ATTTACCTTC ACAAATACAA TCATCTTTCG AAAATTCAGA GGGTCCTAGA
    AAAGATGTCT TAACTCCTTC AGAGGCATAT GAAGAACTTC TAAACCAAAG ACGACTAGAA
    GAGGACAGGT ACCGAAAATT AGATGATGAA ATTGAATTAA GGAACAATAG AAGAGTCATT
    AAAAAGACTG ATGAAGAAGT GGGCGTTTCC AGTAAAAAAC ATCAAAGGTT TGACAGCACG
    ACTGACATTC CAGATAGAAG ATTTCACAGG TTTAATGAGG ATCTTGCTTT TGATAAACAG
    TCTTACAGAC CAGGCTATGA TCCAGAAGTA AGTGAAGACA TGGATGAGAG GTTTAGATAT
    GAAAGTGATT TTGATAGAAG ACTTTTGAGA GTTTATACAA AAAACAGGAT GTACGGGAAC
    AGACGAGGCA ATGAGCCTCC TATGGAGTGT GATGGTGATG TCACAGAACA GTCAAACATA
    CGAATTTCAT CTGCTGGAAA TCAAAGTGCT CGAGACAATG AACCATCCAA ACCTACTTAT
    CGAGAGACAT GCAGTCCTTT TGCGGGAATG CTCTTTGGGG GTGAAGATCG AGAACTTATT
    CAGAGAAGGA AAGAGAAATA TAGACAAGAA CTATTGGAAC AAATGGCTGA ACAACAGAGG
    AACAAGAGAC GAGAAAAAGA TTTGGATCTC AGGGTTGCAG CTTCTGGAGC ACAAGACCCT
    GAGAAATCGC CTGATAGACT AAAGCAGTTT AGTGTGGCAC CAAGACACTT TGAAGAGATG
    ATACCACCTG AGAGACCCAG AATAGCTTTC CAGACACCTC TCCCTCCTCT GTCCGCCCCA
    TCTGTCCCAC CCATCCCATC AGTTTACTCC ATCCCATCTC AGAGTGAAGA TGTGCACAGT
    GGACTCAGCA GCACTCTTGG TGACATGGTG CCTCCCAGAG TTGCACCTCT GCCTCCACCT
    CCCATGCTGC CCCCTTTGGC TGCGAACTAC CGAACTCCTT ACGATGACGC GTACTATTTC
    TATGGGGCCA GGAATACTTT GGATCCTAGT CTTGCTTATT ATGGTTCAGG GATGATGGGA
    GTACAGCCTG CTCCTGCTCC TTATGTTACA GCTCCTGTCA CCCACCAGCC AGTACAGCCT
    ATTGTGAGTA CAGTTGGACA GAATGAGCTG AAAATTACAA GTAATCGAGC AATAAATTCA
    GGATTGTTTT TTGAAGATAA ACCAAAGCCT TCAAAACAGT CATTTCAGTC TTACCAGGAA
    GCTTTGCAGC AGCAGATTCA AGAAAGACAA GAAAGAAGAA AGAAAGAACG TGAAGAAAAA
    GAAGTATATG AAGCCAAATT AGAAGCTGAA ATGAGAAGTT ACAACCCCTG GGGGAAAGGT
    GGAGGTGGTG CTCCTCTGAG AGATGACAAA GGAAATCTGA TAACTGATTT GAATAGGATG
    CACAGACAAA ATATAGATGC CTACCATAAC CCAGATGCAA GAGCATATGA AGATAAAAGG
    GCTGTTGTAT CTCTAGACCA AAATTTAGCC ACTTCAAATG CGGAGAACCA AGAAGATGCT
    GCAAATAAAA ACTCAGGTCA TATGCAATCA CAGAGCTCTC CTTTTGCTCG GGGAAATATT
    TTTAGTGAGC CTCCAACTGA ACTTCAAATT AAACAGCAGG AATTATACAA GAATTTTCTT
    CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA GAAGAAGCAG AGCGAGAGAG ATTAAGAATT
    GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA GAACAGAGAG CTCGAATCCA GCAAGAGTAT
    GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA GAGGAGGAGC AAAGACTGAA AAACGAAGAG
    CTTATTCGGT TAGCTGAAGA AAGACGAAAA GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA
    GAAAAACATA ATCTACAACT TCAGCACTAC TATGAAAGAG AAAACATGAT TGGGGAAGAT
    ACAAAGCACT TGAGACAGCC TTCTCCTGTG GTTCCTGCTC TTCAGAATAA GATTGCAAGC
    AGACTCCAAA GACCTCCTTC AGTTGACAGC ATTATAACTT CCTTTACTCA GGACAGTTCC
    ATGTCCATGG CACAATCTCC TCCGGTACCT GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG
    GAGAAAAAAA ATGTAATTAT GGAATTATCA GAAATGAGAA AACAGCTTCG TAGTGAAGAG
    CGACGTCTGC AGGGGCGGTT ACTACACATT GATAGTGACG ACGACATTCA TATAAGGAAA
    AGAGAAAGTT CCATGGATGT ATTTGATATG GCCAGACATC GATTACAAGC TCCTGTCAGA
    AGACCGTCAC CTAAGAGCTT AGACTCTGCT TCTTCTCAGA ATATTCATGA CTTTAAGGAG
    CTGAAAGATA GAGATTCAGA AACGCGAGCT GATCTGAAAT CTATGTACCC AGATGATCCA
    AGAGATAATG ACACCTTGGA GATAGAACAA CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA
    CTGAATAAAC TAAAAATGCA GGAAGTTGAC TTGGATGCTG TCCCAAGCAC TAAAGTACGG
    GACCACAGAA TGCCCAGAGA GGACACTGAT GATTTCTTGA AAAACTCATT ATTAGAATCT
    GATAGTGCTT TTATTGGTGC GCATGGCGAG ACGTACCCAG CCATTGAGGA TGGGGGCTTC
    CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA GAGCGCAGAA GGAACAAACT GAAAGGATTG
    GACTTTGATA ACAGTCACCC TGATGTGCCA CCAGATGGTG TCTCTTTAAA ATCTGTATCC
    AGTATAAATA TTGATCAGCT TAGAATGAGA AATGAAGAAC GAATGCGAAG ACTGAATGAA
    CTTCAGAATA AACCTATTAC TACAGATGAT GAGAGTTCAC TGGTGGACCC TGATGACATC
    ATGAGGCACC GGGGTGACGA CGGATCAAAC TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA
    GGCACCTCAG AAACACTGAA GCGATTCATG GTGGAACAGC TGAGGCAGGA GCAGCAGCAG
    GGTCCTGGCA AACCAGGCGC TTTCACCTGG CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215536.2
    CDS182..3838
    Translation

    Target ORF information:

    RefSeq Version XM_005215479.4
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215479.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGAAG AATCAAAGAA GTAAAAAACC TATCAGTCAG
    GTTAAACCTG ATTTACCTTC ACAAATACAA TCATCTTTCG AAAATTCAGA GGGTCCTAGA
    AAAGATGTCT TAACTCCTTC AGAGGCATAT GAAGAACTTC TAAACCAAAG ACGACTAGAA
    GAGGACAGGT ACCGAAAATT AGATGATGAA ATTGAATTAA GGAACAATAG AAGAGTCATT
    AAAAAGACTG ATGAAGAAGT GGGCGTTTCC AGTAAAAAAC ATCAAAGGTT TGACAGCACG
    ACTGACATTC CAGATAGAAG ATTTCACAGG TTTAATGAGG ATCTTGCTTT TGATAAACAG
    TCTTACAGAC CAGGCTATGA TCCAGAAGTA AGTGAAGACA TGGATGAGAG GTTTAGATAT
    GAAAGTGATT TTGATAGAAG ACTTTTGAGA GTTTATACAA AAAACAGGAT GTACGGGAAC
    AGACGAGGCA ATGAGCCTCC TATGGAGTGT GATGGTGATG TCACAGAACA GTCAAACATA
    CGAATTTCAT CTGCTGGAAA TCAAAGTGCT CGAGACAATG AACCATCCAA ACCTACTTAT
    CGAGAGACAT GCAGTCCTTT TGCGGGAATG CTCTTTGGGG GTGAAGATCG AGAACTTATT
    CAGAGAAGGA AAGAGAAATA TAGACAAGAA CTATTGGAAC AAATGGCTGA ACAACAGAGG
    AACAAGAGAC GAGAAAAAGA TTTGGATCTC AGGGTTGCAG CTTCTGGAGC ACAAGACCCT
    GAGAAATCGC CTGATAGACT AAAGCAGTTT AGTGTGGCAC CAAGACACTT TGAAGAGATG
    ATACCACCTG AGAGACCCAG AATAGCTTTC CAGACACCTC TCCCTCCTCT GTCCGCCCCA
    TCTGTCCCAC CCATCCCATC AGTTTACTCC ATCCCATCTC AGAGTGAAGA TGTGCACAGT
    GGACTCAGCA GCACTCTTGG TGACATGGTG CCTCCCAGAG TTGCACCTCT GCCTCCACCT
    CCCATGCTGC CCCCTTTGGC TGCGAACTAC CGAACTCCTT ACGATGACGC GTACTATTTC
    TATGGGGCCA GGAATACTTT GGATCCTAGT CTTGCTTATT ATGGTTCAGG GATGATGGGA
    GTACAGCCTG CTCCTGCTCC TTATGTTACA GCTCCTGTCA CCCACCAGCC AGTACAGCCT
    ATTGTGAGTA CAGTTGGACA GAATGAGCTG AAAATTACAA GTAATCGAGC AATAAATTCA
    GGATTGTTTT TTGAAGATAA ACCAAAGCCT TCAAAACAGT CATTTCAGTC TTACCAGGAA
    GCTTTGCAGC AGCAGATTCA AGAAAGACAA GAAAGAAGAA AGAAAGAACG TGAAGAAAAA
    GAAGTATATG AAGCCAAATT AGAAGCTGAA ATGAGAAGTT ACAACCCCTG GGGGAAAGGT
    GGAGGTGGTG CTCCTCTGAG AGATGACAAA GGAAATCTGA TAACTGATTT GAATAGGATG
    CACAGACAAA ATATAGATGC CTACCATAAC CCAGATGCAA GAGCATATGA AGATAAAAGG
    GCTGTTGTAT CTCTAGACCA AAATTTAGCC ACTTCAAATG CGGAGAACCA AGAAGATGCT
    GCAAATAAAA ACTCAGGTCA TATGCAATCA CAGAGCTCTC CTTTTGCTCG GGGAAATATT
    TTTAGTGAGC CTCCAACTGA ACTTCAAATT AAACAGCAGG AATTATACAA GAATTTTCTT
    CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA GAAGAAGCAG AGCGAGAGAG ATTAAGAATT
    GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA GAACAGAGAG CTCGAATCCA GCAAGAGTAT
    GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA GAGGAGGAGC AAAGACTGAA AAACGAAGAG
    CTTATTCGGT TAGCTGAAGA AAGACGAAAA GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA
    GAAAAACATA ATCTACAACT TCAGCACTAC TATGAAAGAG AAAACATGAT TGGGGAAGAT
    ACAAAGCACT TGAGACAGCC TTCTCCTGTG GTTCCTGCTC TTCAGAATAA GATTGCAAGC
    AGACTCCAAA GACCTCCTTC AGTTGACAGC ATTATAACTT CCTTTACTCA GGACAGTTCC
    ATGTCCATGG CACAATCTCC TCCGGTACCT GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG
    GAGAAAAAAA ATGTAATTAT GGAATTATCA GAAATGAGAA AACAGCTTCG TAGTGAAGAG
    CGACGTCTGC AGGGGCGGTT ACTACACATT GATAGTGACG ACGACATTCA TATAAGGAAA
    AGAGAAAGTT CCATGGATGT ATTTGATATG GCCAGACATC GATTACAAGC TCCTGTCAGA
    AGACCGTCAC CTAAGAGCTT AGACTCTGCT TCTTCTCAGA ATATTCATGA CTTTAAGGAG
    CTGAAAGATA GAGATTCAGA AACGCGAGCT GATCTGAAAT CTATGTACCC AGATGATCCA
    AGAGATAATG ACACCTTGGA GATAGAACAA CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA
    CTGAATAAAC TAAAAATGCA GGAAGTTGAC TTGGATGCTG TCCCAAGCAC TAAAGTACGG
    GACCACAGAA TGCCCAGAGA GGACACTGAT GATTTCTTGA AAAACTCATT ATTAGAATCT
    GATAGTGCTT TTATTGGTGC GCATGGCGAG ACGTACCCAG CCATTGAGGA TGGGGGCTTC
    CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA GAGCGCAGAA GGAACAAACT GAAAGGATTG
    GACTTTGATA ACAGTCACCC TGATGTGCCA CCAGATGGTG TCTCTTTAAA ATCTGTATCC
    AGTATAAATA TTGATCAGCT TAGAATGAGA AATGAAGAAC GAATGCGAAG ACTGAATGAA
    CTTCAGAATA AACCTATTAC TACAGATGAT GAGAGTTCAC TGGTGGACCC TGATGACATC
    ATGAGGCACC GGGGTGACGA CGGATCAAAC TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA
    GGCACCTCAG AAACACTGAA GCGATTCATG GTGGAACAGC TGAGGCAGGA GCAGCAGCAG
    GGTCCTGGCA AACCAGGCGC TTTCACCTGG CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50841
    Clone ID Related Accession (Same CDS sequence) XM_005215479.4 , XM_005215479.3
    Accession Version XM_005215479.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGAAG AATCAAAGAA GTAAAAAACC TATCAGTCAG
    GTTAAACCTG ATTTACCTTC ACAAATACAA TCATCTTTCG AAAATTCAGA GGGTCCTAGA
    AAAGATGTCT TAACTCCTTC AGAGGCATAT GAAGAACTTC TAAACCAAAG ACGACTAGAA
    GAGGACAGGT ACCGAAAATT AGATGATGAA ATTGAATTAA GGAACAATAG AAGAGTCATT
    AAAAAGACTG ATGAAGAAGT GGGCGTTTCC AGTAAAAAAC ATCAAAGGTT TGACAGCACG
    ACTGACATTC CAGATAGAAG ATTTCACAGG TTTAATGAGG ATCTTGCTTT TGATAAACAG
    TCTTACAGAC CAGGCTATGA TCCAGAAGTA AGTGAAGACA TGGATGAGAG GTTTAGATAT
    GAAAGTGATT TTGATAGAAG ACTTTTGAGA GTTTATACAA AAAACAGGAT GTACGGGAAC
    AGACGAGGCA ATGAGCCTCC TATGGAGTGT GATGGTGATG TCACAGAACA GTCAAACATA
    CGAATTTCAT CTGCTGGAAA TCAAAGTGCT CGAGACAATG AACCATCCAA ACCTACTTAT
    CGAGAGACAT GCAGTCCTTT TGCGGGAATG CTCTTTGGGG GTGAAGATCG AGAACTTATT
    CAGAGAAGGA AAGAGAAATA TAGACAAGAA CTATTGGAAC AAATGGCTGA ACAACAGAGG
    AACAAGAGAC GAGAAAAAGA TTTGGATCTC AGGGTTGCAG CTTCTGGAGC ACAAGACCCT
    GAGAAATCGC CTGATAGACT AAAGCAGTTT AGTGTGGCAC CAAGACACTT TGAAGAGATG
    ATACCACCTG AGAGACCCAG AATAGCTTTC CAGACACCTC TCCCTCCTCT GTCCGCCCCA
    TCTGTCCCAC CCATCCCATC AGTTTACTCC ATCCCATCTC AGAGTGAAGA TGTGCACAGT
    GGACTCAGCA GCACTCTTGG TGACATGGTG CCTCCCAGAG TTGCACCTCT GCCTCCACCT
    CCCATGCTGC CCCCTTTGGC TGCGAACTAC CGAACTCCTT ACGATGACGC GTACTATTTC
    TATGGGGCCA GGAATACTTT GGATCCTAGT CTTGCTTATT ATGGTTCAGG GATGATGGGA
    GTACAGCCTG CTCCTGCTCC TTATGTTACA GCTCCTGTCA CCCACCAGCC AGTACAGCCT
    ATTGTGAGTA CAGTTGGACA GAATGAGCTG AAAATTACAA GTAATCGAGC AATAAATTCA
    GGATTGTTTT TTGAAGATAA ACCAAAGCCT TCAAAACAGT CATTTCAGTC TTACCAGGAA
    GCTTTGCAGC AGCAGATTCA AGAAAGACAA GAAAGAAGAA AGAAAGAACG TGAAGAAAAA
    GAAGTATATG AAGCCAAATT AGAAGCTGAA ATGAGAAGTT ACAACCCCTG GGGGAAAGGT
    GGAGGTGGTG CTCCTCTGAG AGATGACAAA GGAAATCTGA TAACTGATTT GAATAGGATG
    CACAGACAAA ATATAGATGC CTACCATAAC CCAGATGCAA GAGCATATGA AGATAAAAGG
    GCTGTTGTAT CTCTAGACCA AAATTTAGCC ACTTCAAATG CGGAGAACCA AGAAGATGCT
    GCAAATAAAA ACTCAGGTCA TATGCAATCA CAGAGCTCTC CTTTTGCTCG GGGAAATATT
    TTTAGTGAGC CTCCAACTGA ACTTCAAATT AAACAGCAGG AATTATACAA GAATTTTCTT
    CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA GAAGAAGCAG AGCGAGAGAG ATTAAGAATT
    GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA GAACAGAGAG CTCGAATCCA GCAAGAGTAT
    GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA GAGGAGGAGC AAAGACTGAA AAACGAAGAG
    CTTATTCGGT TAGCTGAAGA AAGACGAAAA GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA
    GAAAAACATA ATCTACAACT TCAGCACTAC TATGAAAGAG AAAACATGAT TGGGGAAGAT
    ACAAAGCACT TGAGACAGCC TTCTCCTGTG GTTCCTGCTC TTCAGAATAA GATTGCAAGC
    AGACTCCAAA GACCTCCTTC AGTTGACAGC ATTATAACTT CCTTTACTCA GGACAGTTCC
    ATGTCCATGG CACAATCTCC TCCGGTACCT GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG
    GAGAAAAAAA ATGTAATTAT GGAATTATCA GAAATGAGAA AACAGCTTCG TAGTGAAGAG
    CGACGTCTGC AGGGGCGGTT ACTACACATT GATAGTGACG ACGACATTCA TATAAGGAAA
    AGAGAAAGTT CCATGGATGT ATTTGATATG GCCAGACATC GATTACAAGC TCCTGTCAGA
    AGACCGTCAC CTAAGAGCTT AGACTCTGCT TCTTCTCAGA ATATTCATGA CTTTAAGGAG
    CTGAAAGATA GAGATTCAGA AACGCGAGCT GATCTGAAAT CTATGTACCC AGATGATCCA
    AGAGATAATG ACACCTTGGA GATAGAACAA CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA
    CTGAATAAAC TAAAAATGCA GGAAGTTGAC TTGGATGCTG TCCCAAGCAC TAAAGTACGG
    GACCACAGAA TGCCCAGAGA GGACACTGAT GATTTCTTGA AAAACTCATT ATTAGAATCT
    GATAGTGCTT TTATTGGTGC GCATGGCGAG ACGTACCCAG CCATTGAGGA TGGGGGCTTC
    CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA GAGCGCAGAA GGAACAAACT GAAAGGATTG
    GACTTTGATA ACAGTCACCC TGATGTGCCA CCAGATGGTG TCTCTTTAAA ATCTGTATCC
    AGTATAAATA TTGATCAGCT TAGAATGAGA AATGAAGAAC GAATGCGAAG ACTGAATGAA
    CTTCAGAATA AACCTATTAC TACAGATGAT GAGAGTTCAC TGGTGGACCC TGATGACATC
    ATGAGGCACC GGGGTGACGA CGGATCAAAC TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA
    GGCACCTCAG AAACACTGAA GCGATTCATG GTGGAACAGC TGAGGCAGGA GCAGCAGCAG
    GGTCCTGGCA AACCAGGCGC TTTCACCTGG CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215536.2
    CDS114..3770
    Translation

    Target ORF information:

    RefSeq Version XM_005215479.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215479.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGAAG AATCAAAGAA GTAAAAAACC TATCAGTCAG
    GTTAAACCTG ATTTACCTTC ACAAATACAA TCATCTTTCG AAAATTCAGA GGGTCCTAGA
    AAAGATGTCT TAACTCCTTC AGAGGCATAT GAAGAACTTC TAAACCAAAG ACGACTAGAA
    GAGGACAGGT ACCGAAAATT AGATGATGAA ATTGAATTAA GGAACAATAG AAGAGTCATT
    AAAAAGACTG ATGAAGAAGT GGGCGTTTCC AGTAAAAAAC ATCAAAGGTT TGACAGCACG
    ACTGACATTC CAGATAGAAG ATTTCACAGG TTTAATGAGG ATCTTGCTTT TGATAAACAG
    TCTTACAGAC CAGGCTATGA TCCAGAAGTA AGTGAAGACA TGGATGAGAG GTTTAGATAT
    GAAAGTGATT TTGATAGAAG ACTTTTGAGA GTTTATACAA AAAACAGGAT GTACGGGAAC
    AGACGAGGCA ATGAGCCTCC TATGGAGTGT GATGGTGATG TCACAGAACA GTCAAACATA
    CGAATTTCAT CTGCTGGAAA TCAAAGTGCT CGAGACAATG AACCATCCAA ACCTACTTAT
    CGAGAGACAT GCAGTCCTTT TGCGGGAATG CTCTTTGGGG GTGAAGATCG AGAACTTATT
    CAGAGAAGGA AAGAGAAATA TAGACAAGAA CTATTGGAAC AAATGGCTGA ACAACAGAGG
    AACAAGAGAC GAGAAAAAGA TTTGGATCTC AGGGTTGCAG CTTCTGGAGC ACAAGACCCT
    GAGAAATCGC CTGATAGACT AAAGCAGTTT AGTGTGGCAC CAAGACACTT TGAAGAGATG
    ATACCACCTG AGAGACCCAG AATAGCTTTC CAGACACCTC TCCCTCCTCT GTCCGCCCCA
    TCTGTCCCAC CCATCCCATC AGTTTACTCC ATCCCATCTC AGAGTGAAGA TGTGCACAGT
    GGACTCAGCA GCACTCTTGG TGACATGGTG CCTCCCAGAG TTGCACCTCT GCCTCCACCT
    CCCATGCTGC CCCCTTTGGC TGCGAACTAC CGAACTCCTT ACGATGACGC GTACTATTTC
    TATGGGGCCA GGAATACTTT GGATCCTAGT CTTGCTTATT ATGGTTCAGG GATGATGGGA
    GTACAGCCTG CTCCTGCTCC TTATGTTACA GCTCCTGTCA CCCACCAGCC AGTACAGCCT
    ATTGTGAGTA CAGTTGGACA GAATGAGCTG AAAATTACAA GTAATCGAGC AATAAATTCA
    GGATTGTTTT TTGAAGATAA ACCAAAGCCT TCAAAACAGT CATTTCAGTC TTACCAGGAA
    GCTTTGCAGC AGCAGATTCA AGAAAGACAA GAAAGAAGAA AGAAAGAACG TGAAGAAAAA
    GAAGTATATG AAGCCAAATT AGAAGCTGAA ATGAGAAGTT ACAACCCCTG GGGGAAAGGT
    GGAGGTGGTG CTCCTCTGAG AGATGACAAA GGAAATCTGA TAACTGATTT GAATAGGATG
    CACAGACAAA ATATAGATGC CTACCATAAC CCAGATGCAA GAGCATATGA AGATAAAAGG
    GCTGTTGTAT CTCTAGACCA AAATTTAGCC ACTTCAAATG CGGAGAACCA AGAAGATGCT
    GCAAATAAAA ACTCAGGTCA TATGCAATCA CAGAGCTCTC CTTTTGCTCG GGGAAATATT
    TTTAGTGAGC CTCCAACTGA ACTTCAAATT AAACAGCAGG AATTATACAA GAATTTTCTT
    CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA GAAGAAGCAG AGCGAGAGAG ATTAAGAATT
    GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA GAACAGAGAG CTCGAATCCA GCAAGAGTAT
    GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA GAGGAGGAGC AAAGACTGAA AAACGAAGAG
    CTTATTCGGT TAGCTGAAGA AAGACGAAAA GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA
    GAAAAACATA ATCTACAACT TCAGCACTAC TATGAAAGAG AAAACATGAT TGGGGAAGAT
    ACAAAGCACT TGAGACAGCC TTCTCCTGTG GTTCCTGCTC TTCAGAATAA GATTGCAAGC
    AGACTCCAAA GACCTCCTTC AGTTGACAGC ATTATAACTT CCTTTACTCA GGACAGTTCC
    ATGTCCATGG CACAATCTCC TCCGGTACCT GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG
    GAGAAAAAAA ATGTAATTAT GGAATTATCA GAAATGAGAA AACAGCTTCG TAGTGAAGAG
    CGACGTCTGC AGGGGCGGTT ACTACACATT GATAGTGACG ACGACATTCA TATAAGGAAA
    AGAGAAAGTT CCATGGATGT ATTTGATATG GCCAGACATC GATTACAAGC TCCTGTCAGA
    AGACCGTCAC CTAAGAGCTT AGACTCTGCT TCTTCTCAGA ATATTCATGA CTTTAAGGAG
    CTGAAAGATA GAGATTCAGA AACGCGAGCT GATCTGAAAT CTATGTACCC AGATGATCCA
    AGAGATAATG ACACCTTGGA GATAGAACAA CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA
    CTGAATAAAC TAAAAATGCA GGAAGTTGAC TTGGATGCTG TCCCAAGCAC TAAAGTACGG
    GACCACAGAA TGCCCAGAGA GGACACTGAT GATTTCTTGA AAAACTCATT ATTAGAATCT
    GATAGTGCTT TTATTGGTGC GCATGGCGAG ACGTACCCAG CCATTGAGGA TGGGGGCTTC
    CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA GAGCGCAGAA GGAACAAACT GAAAGGATTG
    GACTTTGATA ACAGTCACCC TGATGTGCCA CCAGATGGTG TCTCTTTAAA ATCTGTATCC
    AGTATAAATA TTGATCAGCT TAGAATGAGA AATGAAGAAC GAATGCGAAG ACTGAATGAA
    CTTCAGAATA AACCTATTAC TACAGATGAT GAGAGTTCAC TGGTGGACCC TGATGACATC
    ATGAGGCACC GGGGTGACGA CGGATCAAAC TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA
    GGCACCTCAG AAACACTGAA GCGATTCATG GTGGAACAGC TGAGGCAGGA GCAGCAGCAG
    GGTCCTGGCA AACCAGGCGC TTTCACCTGG CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50842
    Clone ID Related Accession (Same CDS sequence) XM_005215480.3 , XM_005215480.4
    Accession Version XM_005215480.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGCAGAAGA ATCAAAGAAG TAAAAAACCT ATCAGTCAGG TTAAACCTGA TTTACCTTCA
    CAAATACAAT CATCTTTCGA AAATTCAGAG GGTCCTAGAA AAGATGTCTT AACTCCTTCA
    GAGGCATATG AAGAACTTCT AAACCAAAGA CGACTAGAAG AGGACAGGTA CCGAAAATTA
    GATGATGAAA TTGAATTAAG GAACAATAGA AGAGTCATTA AAAAGACTGA TGAAGAAGTG
    GGCGTTTCCA GTAAAAAACA TCAAAGGTTT GACAGCACGA CTGACATTCC AGATAGAAGA
    TTTCACAGGT TTAATGAGGA TCTTGCTTTT GATAAACAGT CTTACAGACC AGGCTATGAT
    CCAGAAGTAA GTGAAGACAT GGATGAGAGG TTTAGATATG AAAGTGATTT TGATAGAAGA
    CTTTTGAGAG TTTATACAAA AAACAGGATG TACGGGAACA GACGAGGCAA TGAGCCTCCT
    ATGGAGTGTG ATGGTGATGT CACAGAACAG TCAAACATAC GAATTTCATC TGCTGGAAAT
    CAAAGTGCTC GAGACAATGA ACCATCCAAA CCTACTTATC GAGAGACATG CAGTCCTTTT
    GCGGGAATGC TCTTTGGGGG TGAAGATCGA GAACTTATTC AGAGAAGGAA AGAGAAATAT
    AGACAAGAAC TATTGGAACA AATGGCTGAA CAACAGAGGA ACAAGAGACG AGAAAAAGAT
    TTGGATCTCA GGGTTGCAGC TTCTGGAGCA CAAGACCCTG AGAAATCGCC TGATAGACTA
    AAGCAGTTTA GTGTGGCACC AAGACACTTT GAAGAGATGA TACCACCTGA GAGACCCAGA
    ATAGCTTTCC AGACACCTCT CCCTCCTCTG TCCGCCCCAT CTGTCCCACC CATCCCATCA
    GTTTACTCCA TCCCATCTCA GAGTGAAGAT GTGCACAGTG GACTCAGCAG CACTCTTGGT
    GACATGGTGC CTCCCAGAGT TGCACCTCTG CCTCCACCTC CCATGCTGCC CCCTTTGGCT
    GCGAACTACC GAACTCCTTA CGATGACGCG TACTATTTCT ATGGGGCCAG GAATACTTTG
    GATCCTAGTC TTGCTTATTA TGGTTCAGGG ATGATGGGAG TACAGCCTGC TCCTGCTCCT
    TATGTTACAG CTCCTGTCAC CCACCAGCCA GTACAGCCTA TTGTGAGTAC AGTTGGACAG
    AATGAGCTGA AAATTACAAG TAATCGAGCA ATAAATTCAG GATTGTTTTT TGAAGATAAA
    CCAAAGCCTT CAAAACAGTC ATTTCAGTCT TACCAGGAAG CTTTGCAGCA GCAGATTCAA
    GAAAGACAAG AAAGAAGAAA GAAAGAACGT GAAGAAAAAG AAGTATATGA AGCCAAATTA
    GAAGCTGAAA TGAGAAGTTA CAACCCCTGG GGGAAAGGTG GAGGTGGTGC TCCTCTGAGA
    GATGACAAAG GAAATCTGAT AACTGATTTG AATAGGATGC ACAGACAAAA TATAGATGCC
    TACCATAACC CAGATGCAAG AGCATATGAA GATAAAAGGG CTGTTGTATC TCTAGACCAA
    AATTTAGCCA CTTCAAATGC GGAGAACCAA GAAGATGCTG CAAATAAAAA CTCAGGTCAT
    ATGCAATCAC AGAGCTCTCC TTTTGCTCGG GGAAATATTT TTAGTGAGCC TCCAACTGAA
    CTTCAAATTA AACAGCAGGA ATTATACAAG AATTTTCTTC GTTTTCAGAT TGAGGAAAAG
    AAGCAAAGAG AAGAAGCAGA GCGAGAGAGA TTAAGAATTG CTGAAGAAAA AGAAGAAAAA
    CGGCTTGCAG AACAGAGAGC TCGAATCCAG CAAGAGTATG AAGAGGAGCA GGAAAAGAAA
    AGGGAGAAAG AGGAGGAGCA AAGACTGAAA AACGAAGAGC TTATTCGGTT AGCTGAAGAA
    AGACGAAAAG AAGCAGAAAG AAAGAAGAAA GAAGAAGAAG AAAAACATAA TCTACAACTT
    CAGCACTACT ATGAAAGAGA AAACATGATT GGGGAAGATA CAAAGCACTT GAGACAGCCT
    TCTCCTGTGG TTCCTGCTCT TCAGAATAAG ATTGCAAGCA GACTCCAAAG ACCTCCTTCA
    GTTGACAGCA TTATAACTTC CTTTACTCAG GACAGTTCCA TGTCCATGGC ACAATCTCCT
    CCGGTACCTG CCAGGAAAAA CCAGCTTCGT GCAGAAGAGG AGAAAAAAAA TGTAATTATG
    GAATTATCAG AAATGAGAAA ACAGCTTCGT AGTGAAGAGC GACGTCTGCA GGGGCGGTTA
    CTACACATTG ATAGTGACGA CGACATTCAT ATAAGGAAAA GAGAAAGTTC CATGGATGTA
    TTTGATATGG CCAGACATCG ATTACAAGCT CCTGTCAGAA GACCGTCACC TAAGAGCTTA
    GACTCTGCTT CTTCTCAGAA TATTCATGAC TTTAAGGAGC TGAAAGATAG AGATTCAGAA
    ACGCGAGCTG ATCTGAAATC TATGTACCCA GATGATCCAA GAGATAATGA CACCTTGGAG
    ATAGAACAAC AAGCCTTGCT AAGAGAGCAG CAGAAGAGAC TGAATAAACT AAAAATGCAG
    GAAGTTGACT TGGATGCTGT CCCAAGCACT AAAGTACGGG ACCACAGAAT GCCCAGAGAG
    GACACTGATG ATTTCTTGAA AAACTCATTA TTAGAATCTG ATAGTGCTTT TATTGGTGCG
    CATGGCGAGA CGTACCCAGC CATTGAGGAT GGGGGCTTCC TGCTGCTATC TCCGCTGCCC
    TCCGCCAGAG AGCGCAGAAG GAACAAACTG AAAGGATTGG ACTTTGATAA CAGTCACCCT
    GATGTGCCAC CAGATGGTGT CTCTTTAAAA TCTGTATCCA GTATAAATAT TGATCAGCTT
    AGAATGAGAA ATGAAGAACG AATGCGAAGA CTGAATGAAC TTCAGAATAA ACCTATTACT
    ACAGATGATG AGAGTTCACT GGTGGACCCT GATGACATCA TGAGGCACCG GGGTGACGAC
    GGATCAAACT CTGTAGCCAC TGAGCCCTGG CTCCGCCCAG GCACCTCAGA AACACTGAAG
    CGATTCATGG TGGAACAGCT GAGGCAGGAG CAGCAGCAGG GTCCTGGCAA ACCAGGCGCT
    TTCACCTGGC AGGGACTGTC TACTGCACAC GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215537.2
    CDS114..3749
    Translation

    Target ORF information:

    RefSeq Version XM_005215480.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215480.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGCAGAAGA ATCAAAGAAG TAAAAAACCT ATCAGTCAGG TTAAACCTGA TTTACCTTCA
    CAAATACAAT CATCTTTCGA AAATTCAGAG GGTCCTAGAA AAGATGTCTT AACTCCTTCA
    GAGGCATATG AAGAACTTCT AAACCAAAGA CGACTAGAAG AGGACAGGTA CCGAAAATTA
    GATGATGAAA TTGAATTAAG GAACAATAGA AGAGTCATTA AAAAGACTGA TGAAGAAGTG
    GGCGTTTCCA GTAAAAAACA TCAAAGGTTT GACAGCACGA CTGACATTCC AGATAGAAGA
    TTTCACAGGT TTAATGAGGA TCTTGCTTTT GATAAACAGT CTTACAGACC AGGCTATGAT
    CCAGAAGTAA GTGAAGACAT GGATGAGAGG TTTAGATATG AAAGTGATTT TGATAGAAGA
    CTTTTGAGAG TTTATACAAA AAACAGGATG TACGGGAACA GACGAGGCAA TGAGCCTCCT
    ATGGAGTGTG ATGGTGATGT CACAGAACAG TCAAACATAC GAATTTCATC TGCTGGAAAT
    CAAAGTGCTC GAGACAATGA ACCATCCAAA CCTACTTATC GAGAGACATG CAGTCCTTTT
    GCGGGAATGC TCTTTGGGGG TGAAGATCGA GAACTTATTC AGAGAAGGAA AGAGAAATAT
    AGACAAGAAC TATTGGAACA AATGGCTGAA CAACAGAGGA ACAAGAGACG AGAAAAAGAT
    TTGGATCTCA GGGTTGCAGC TTCTGGAGCA CAAGACCCTG AGAAATCGCC TGATAGACTA
    AAGCAGTTTA GTGTGGCACC AAGACACTTT GAAGAGATGA TACCACCTGA GAGACCCAGA
    ATAGCTTTCC AGACACCTCT CCCTCCTCTG TCCGCCCCAT CTGTCCCACC CATCCCATCA
    GTTTACTCCA TCCCATCTCA GAGTGAAGAT GTGCACAGTG GACTCAGCAG CACTCTTGGT
    GACATGGTGC CTCCCAGAGT TGCACCTCTG CCTCCACCTC CCATGCTGCC CCCTTTGGCT
    GCGAACTACC GAACTCCTTA CGATGACGCG TACTATTTCT ATGGGGCCAG GAATACTTTG
    GATCCTAGTC TTGCTTATTA TGGTTCAGGG ATGATGGGAG TACAGCCTGC TCCTGCTCCT
    TATGTTACAG CTCCTGTCAC CCACCAGCCA GTACAGCCTA TTGTGAGTAC AGTTGGACAG
    AATGAGCTGA AAATTACAAG TAATCGAGCA ATAAATTCAG GATTGTTTTT TGAAGATAAA
    CCAAAGCCTT CAAAACAGTC ATTTCAGTCT TACCAGGAAG CTTTGCAGCA GCAGATTCAA
    GAAAGACAAG AAAGAAGAAA GAAAGAACGT GAAGAAAAAG AAGTATATGA AGCCAAATTA
    GAAGCTGAAA TGAGAAGTTA CAACCCCTGG GGGAAAGGTG GAGGTGGTGC TCCTCTGAGA
    GATGACAAAG GAAATCTGAT AACTGATTTG AATAGGATGC ACAGACAAAA TATAGATGCC
    TACCATAACC CAGATGCAAG AGCATATGAA GATAAAAGGG CTGTTGTATC TCTAGACCAA
    AATTTAGCCA CTTCAAATGC GGAGAACCAA GAAGATGCTG CAAATAAAAA CTCAGGTCAT
    ATGCAATCAC AGAGCTCTCC TTTTGCTCGG GGAAATATTT TTAGTGAGCC TCCAACTGAA
    CTTCAAATTA AACAGCAGGA ATTATACAAG AATTTTCTTC GTTTTCAGAT TGAGGAAAAG
    AAGCAAAGAG AAGAAGCAGA GCGAGAGAGA TTAAGAATTG CTGAAGAAAA AGAAGAAAAA
    CGGCTTGCAG AACAGAGAGC TCGAATCCAG CAAGAGTATG AAGAGGAGCA GGAAAAGAAA
    AGGGAGAAAG AGGAGGAGCA AAGACTGAAA AACGAAGAGC TTATTCGGTT AGCTGAAGAA
    AGACGAAAAG AAGCAGAAAG AAAGAAGAAA GAAGAAGAAG AAAAACATAA TCTACAACTT
    CAGCACTACT ATGAAAGAGA AAACATGATT GGGGAAGATA CAAAGCACTT GAGACAGCCT
    TCTCCTGTGG TTCCTGCTCT TCAGAATAAG ATTGCAAGCA GACTCCAAAG ACCTCCTTCA
    GTTGACAGCA TTATAACTTC CTTTACTCAG GACAGTTCCA TGTCCATGGC ACAATCTCCT
    CCGGTACCTG CCAGGAAAAA CCAGCTTCGT GCAGAAGAGG AGAAAAAAAA TGTAATTATG
    GAATTATCAG AAATGAGAAA ACAGCTTCGT AGTGAAGAGC GACGTCTGCA GGGGCGGTTA
    CTACACATTG ATAGTGACGA CGACATTCAT ATAAGGAAAA GAGAAAGTTC CATGGATGTA
    TTTGATATGG CCAGACATCG ATTACAAGCT CCTGTCAGAA GACCGTCACC TAAGAGCTTA
    GACTCTGCTT CTTCTCAGAA TATTCATGAC TTTAAGGAGC TGAAAGATAG AGATTCAGAA
    ACGCGAGCTG ATCTGAAATC TATGTACCCA GATGATCCAA GAGATAATGA CACCTTGGAG
    ATAGAACAAC AAGCCTTGCT AAGAGAGCAG CAGAAGAGAC TGAATAAACT AAAAATGCAG
    GAAGTTGACT TGGATGCTGT CCCAAGCACT AAAGTACGGG ACCACAGAAT GCCCAGAGAG
    GACACTGATG ATTTCTTGAA AAACTCATTA TTAGAATCTG ATAGTGCTTT TATTGGTGCG
    CATGGCGAGA CGTACCCAGC CATTGAGGAT GGGGGCTTCC TGCTGCTATC TCCGCTGCCC
    TCCGCCAGAG AGCGCAGAAG GAACAAACTG AAAGGATTGG ACTTTGATAA CAGTCACCCT
    GATGTGCCAC CAGATGGTGT CTCTTTAAAA TCTGTATCCA GTATAAATAT TGATCAGCTT
    AGAATGAGAA ATGAAGAACG AATGCGAAGA CTGAATGAAC TTCAGAATAA ACCTATTACT
    ACAGATGATG AGAGTTCACT GGTGGACCCT GATGACATCA TGAGGCACCG GGGTGACGAC
    GGATCAAACT CTGTAGCCAC TGAGCCCTGG CTCCGCCCAG GCACCTCAGA AACACTGAAG
    CGATTCATGG TGGAACAGCT GAGGCAGGAG CAGCAGCAGG GTCCTGGCAA ACCAGGCGCT
    TTCACCTGGC AGGGACTGTC TACTGCACAC GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50842
    Clone ID Related Accession (Same CDS sequence) XM_005215480.3 , XM_005215480.4
    Accession Version XM_005215480.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215480.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGCAGAAGA ATCAAAGAAG TAAAAAACCT ATCAGTCAGG TTAAACCTGA TTTACCTTCA
    CAAATACAAT CATCTTTCGA AAATTCAGAG GGTCCTAGAA AAGATGTCTT AACTCCTTCA
    GAGGCATATG AAGAACTTCT AAACCAAAGA CGACTAGAAG AGGACAGGTA CCGAAAATTA
    GATGATGAAA TTGAATTAAG GAACAATAGA AGAGTCATTA AAAAGACTGA TGAAGAAGTG
    GGCGTTTCCA GTAAAAAACA TCAAAGGTTT GACAGCACGA CTGACATTCC AGATAGAAGA
    TTTCACAGGT TTAATGAGGA TCTTGCTTTT GATAAACAGT CTTACAGACC AGGCTATGAT
    CCAGAAGTAA GTGAAGACAT GGATGAGAGG TTTAGATATG AAAGTGATTT TGATAGAAGA
    CTTTTGAGAG TTTATACAAA AAACAGGATG TACGGGAACA GACGAGGCAA TGAGCCTCCT
    ATGGAGTGTG ATGGTGATGT CACAGAACAG TCAAACATAC GAATTTCATC TGCTGGAAAT
    CAAAGTGCTC GAGACAATGA ACCATCCAAA CCTACTTATC GAGAGACATG CAGTCCTTTT
    GCGGGAATGC TCTTTGGGGG TGAAGATCGA GAACTTATTC AGAGAAGGAA AGAGAAATAT
    AGACAAGAAC TATTGGAACA AATGGCTGAA CAACAGAGGA ACAAGAGACG AGAAAAAGAT
    TTGGATCTCA GGGTTGCAGC TTCTGGAGCA CAAGACCCTG AGAAATCGCC TGATAGACTA
    AAGCAGTTTA GTGTGGCACC AAGACACTTT GAAGAGATGA TACCACCTGA GAGACCCAGA
    ATAGCTTTCC AGACACCTCT CCCTCCTCTG TCCGCCCCAT CTGTCCCACC CATCCCATCA
    GTTTACTCCA TCCCATCTCA GAGTGAAGAT GTGCACAGTG GACTCAGCAG CACTCTTGGT
    GACATGGTGC CTCCCAGAGT TGCACCTCTG CCTCCACCTC CCATGCTGCC CCCTTTGGCT
    GCGAACTACC GAACTCCTTA CGATGACGCG TACTATTTCT ATGGGGCCAG GAATACTTTG
    GATCCTAGTC TTGCTTATTA TGGTTCAGGG ATGATGGGAG TACAGCCTGC TCCTGCTCCT
    TATGTTACAG CTCCTGTCAC CCACCAGCCA GTACAGCCTA TTGTGAGTAC AGTTGGACAG
    AATGAGCTGA AAATTACAAG TAATCGAGCA ATAAATTCAG GATTGTTTTT TGAAGATAAA
    CCAAAGCCTT CAAAACAGTC ATTTCAGTCT TACCAGGAAG CTTTGCAGCA GCAGATTCAA
    GAAAGACAAG AAAGAAGAAA GAAAGAACGT GAAGAAAAAG AAGTATATGA AGCCAAATTA
    GAAGCTGAAA TGAGAAGTTA CAACCCCTGG GGGAAAGGTG GAGGTGGTGC TCCTCTGAGA
    GATGACAAAG GAAATCTGAT AACTGATTTG AATAGGATGC ACAGACAAAA TATAGATGCC
    TACCATAACC CAGATGCAAG AGCATATGAA GATAAAAGGG CTGTTGTATC TCTAGACCAA
    AATTTAGCCA CTTCAAATGC GGAGAACCAA GAAGATGCTG CAAATAAAAA CTCAGGTCAT
    ATGCAATCAC AGAGCTCTCC TTTTGCTCGG GGAAATATTT TTAGTGAGCC TCCAACTGAA
    CTTCAAATTA AACAGCAGGA ATTATACAAG AATTTTCTTC GTTTTCAGAT TGAGGAAAAG
    AAGCAAAGAG AAGAAGCAGA GCGAGAGAGA TTAAGAATTG CTGAAGAAAA AGAAGAAAAA
    CGGCTTGCAG AACAGAGAGC TCGAATCCAG CAAGAGTATG AAGAGGAGCA GGAAAAGAAA
    AGGGAGAAAG AGGAGGAGCA AAGACTGAAA AACGAAGAGC TTATTCGGTT AGCTGAAGAA
    AGACGAAAAG AAGCAGAAAG AAAGAAGAAA GAAGAAGAAG AAAAACATAA TCTACAACTT
    CAGCACTACT ATGAAAGAGA AAACATGATT GGGGAAGATA CAAAGCACTT GAGACAGCCT
    TCTCCTGTGG TTCCTGCTCT TCAGAATAAG ATTGCAAGCA GACTCCAAAG ACCTCCTTCA
    GTTGACAGCA TTATAACTTC CTTTACTCAG GACAGTTCCA TGTCCATGGC ACAATCTCCT
    CCGGTACCTG CCAGGAAAAA CCAGCTTCGT GCAGAAGAGG AGAAAAAAAA TGTAATTATG
    GAATTATCAG AAATGAGAAA ACAGCTTCGT AGTGAAGAGC GACGTCTGCA GGGGCGGTTA
    CTACACATTG ATAGTGACGA CGACATTCAT ATAAGGAAAA GAGAAAGTTC CATGGATGTA
    TTTGATATGG CCAGACATCG ATTACAAGCT CCTGTCAGAA GACCGTCACC TAAGAGCTTA
    GACTCTGCTT CTTCTCAGAA TATTCATGAC TTTAAGGAGC TGAAAGATAG AGATTCAGAA
    ACGCGAGCTG ATCTGAAATC TATGTACCCA GATGATCCAA GAGATAATGA CACCTTGGAG
    ATAGAACAAC AAGCCTTGCT AAGAGAGCAG CAGAAGAGAC TGAATAAACT AAAAATGCAG
    GAAGTTGACT TGGATGCTGT CCCAAGCACT AAAGTACGGG ACCACAGAAT GCCCAGAGAG
    GACACTGATG ATTTCTTGAA AAACTCATTA TTAGAATCTG ATAGTGCTTT TATTGGTGCG
    CATGGCGAGA CGTACCCAGC CATTGAGGAT GGGGGCTTCC TGCTGCTATC TCCGCTGCCC
    TCCGCCAGAG AGCGCAGAAG GAACAAACTG AAAGGATTGG ACTTTGATAA CAGTCACCCT
    GATGTGCCAC CAGATGGTGT CTCTTTAAAA TCTGTATCCA GTATAAATAT TGATCAGCTT
    AGAATGAGAA ATGAAGAACG AATGCGAAGA CTGAATGAAC TTCAGAATAA ACCTATTACT
    ACAGATGATG AGAGTTCACT GGTGGACCCT GATGACATCA TGAGGCACCG GGGTGACGAC
    GGATCAAACT CTGTAGCCAC TGAGCCCTGG CTCCGCCCAG GCACCTCAGA AACACTGAAG
    CGATTCATGG TGGAACAGCT GAGGCAGGAG CAGCAGCAGG GTCCTGGCAA ACCAGGCGCT
    TTCACCTGGC AGGGACTGTC TACTGCACAC GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215537.2
    CDS182..3817
    Translation

    Target ORF information:

    RefSeq Version XM_005215480.4
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215480.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGCAGAAGA ATCAAAGAAG TAAAAAACCT ATCAGTCAGG TTAAACCTGA TTTACCTTCA
    CAAATACAAT CATCTTTCGA AAATTCAGAG GGTCCTAGAA AAGATGTCTT AACTCCTTCA
    GAGGCATATG AAGAACTTCT AAACCAAAGA CGACTAGAAG AGGACAGGTA CCGAAAATTA
    GATGATGAAA TTGAATTAAG GAACAATAGA AGAGTCATTA AAAAGACTGA TGAAGAAGTG
    GGCGTTTCCA GTAAAAAACA TCAAAGGTTT GACAGCACGA CTGACATTCC AGATAGAAGA
    TTTCACAGGT TTAATGAGGA TCTTGCTTTT GATAAACAGT CTTACAGACC AGGCTATGAT
    CCAGAAGTAA GTGAAGACAT GGATGAGAGG TTTAGATATG AAAGTGATTT TGATAGAAGA
    CTTTTGAGAG TTTATACAAA AAACAGGATG TACGGGAACA GACGAGGCAA TGAGCCTCCT
    ATGGAGTGTG ATGGTGATGT CACAGAACAG TCAAACATAC GAATTTCATC TGCTGGAAAT
    CAAAGTGCTC GAGACAATGA ACCATCCAAA CCTACTTATC GAGAGACATG CAGTCCTTTT
    GCGGGAATGC TCTTTGGGGG TGAAGATCGA GAACTTATTC AGAGAAGGAA AGAGAAATAT
    AGACAAGAAC TATTGGAACA AATGGCTGAA CAACAGAGGA ACAAGAGACG AGAAAAAGAT
    TTGGATCTCA GGGTTGCAGC TTCTGGAGCA CAAGACCCTG AGAAATCGCC TGATAGACTA
    AAGCAGTTTA GTGTGGCACC AAGACACTTT GAAGAGATGA TACCACCTGA GAGACCCAGA
    ATAGCTTTCC AGACACCTCT CCCTCCTCTG TCCGCCCCAT CTGTCCCACC CATCCCATCA
    GTTTACTCCA TCCCATCTCA GAGTGAAGAT GTGCACAGTG GACTCAGCAG CACTCTTGGT
    GACATGGTGC CTCCCAGAGT TGCACCTCTG CCTCCACCTC CCATGCTGCC CCCTTTGGCT
    GCGAACTACC GAACTCCTTA CGATGACGCG TACTATTTCT ATGGGGCCAG GAATACTTTG
    GATCCTAGTC TTGCTTATTA TGGTTCAGGG ATGATGGGAG TACAGCCTGC TCCTGCTCCT
    TATGTTACAG CTCCTGTCAC CCACCAGCCA GTACAGCCTA TTGTGAGTAC AGTTGGACAG
    AATGAGCTGA AAATTACAAG TAATCGAGCA ATAAATTCAG GATTGTTTTT TGAAGATAAA
    CCAAAGCCTT CAAAACAGTC ATTTCAGTCT TACCAGGAAG CTTTGCAGCA GCAGATTCAA
    GAAAGACAAG AAAGAAGAAA GAAAGAACGT GAAGAAAAAG AAGTATATGA AGCCAAATTA
    GAAGCTGAAA TGAGAAGTTA CAACCCCTGG GGGAAAGGTG GAGGTGGTGC TCCTCTGAGA
    GATGACAAAG GAAATCTGAT AACTGATTTG AATAGGATGC ACAGACAAAA TATAGATGCC
    TACCATAACC CAGATGCAAG AGCATATGAA GATAAAAGGG CTGTTGTATC TCTAGACCAA
    AATTTAGCCA CTTCAAATGC GGAGAACCAA GAAGATGCTG CAAATAAAAA CTCAGGTCAT
    ATGCAATCAC AGAGCTCTCC TTTTGCTCGG GGAAATATTT TTAGTGAGCC TCCAACTGAA
    CTTCAAATTA AACAGCAGGA ATTATACAAG AATTTTCTTC GTTTTCAGAT TGAGGAAAAG
    AAGCAAAGAG AAGAAGCAGA GCGAGAGAGA TTAAGAATTG CTGAAGAAAA AGAAGAAAAA
    CGGCTTGCAG AACAGAGAGC TCGAATCCAG CAAGAGTATG AAGAGGAGCA GGAAAAGAAA
    AGGGAGAAAG AGGAGGAGCA AAGACTGAAA AACGAAGAGC TTATTCGGTT AGCTGAAGAA
    AGACGAAAAG AAGCAGAAAG AAAGAAGAAA GAAGAAGAAG AAAAACATAA TCTACAACTT
    CAGCACTACT ATGAAAGAGA AAACATGATT GGGGAAGATA CAAAGCACTT GAGACAGCCT
    TCTCCTGTGG TTCCTGCTCT TCAGAATAAG ATTGCAAGCA GACTCCAAAG ACCTCCTTCA
    GTTGACAGCA TTATAACTTC CTTTACTCAG GACAGTTCCA TGTCCATGGC ACAATCTCCT
    CCGGTACCTG CCAGGAAAAA CCAGCTTCGT GCAGAAGAGG AGAAAAAAAA TGTAATTATG
    GAATTATCAG AAATGAGAAA ACAGCTTCGT AGTGAAGAGC GACGTCTGCA GGGGCGGTTA
    CTACACATTG ATAGTGACGA CGACATTCAT ATAAGGAAAA GAGAAAGTTC CATGGATGTA
    TTTGATATGG CCAGACATCG ATTACAAGCT CCTGTCAGAA GACCGTCACC TAAGAGCTTA
    GACTCTGCTT CTTCTCAGAA TATTCATGAC TTTAAGGAGC TGAAAGATAG AGATTCAGAA
    ACGCGAGCTG ATCTGAAATC TATGTACCCA GATGATCCAA GAGATAATGA CACCTTGGAG
    ATAGAACAAC AAGCCTTGCT AAGAGAGCAG CAGAAGAGAC TGAATAAACT AAAAATGCAG
    GAAGTTGACT TGGATGCTGT CCCAAGCACT AAAGTACGGG ACCACAGAAT GCCCAGAGAG
    GACACTGATG ATTTCTTGAA AAACTCATTA TTAGAATCTG ATAGTGCTTT TATTGGTGCG
    CATGGCGAGA CGTACCCAGC CATTGAGGAT GGGGGCTTCC TGCTGCTATC TCCGCTGCCC
    TCCGCCAGAG AGCGCAGAAG GAACAAACTG AAAGGATTGG ACTTTGATAA CAGTCACCCT
    GATGTGCCAC CAGATGGTGT CTCTTTAAAA TCTGTATCCA GTATAAATAT TGATCAGCTT
    AGAATGAGAA ATGAAGAACG AATGCGAAGA CTGAATGAAC TTCAGAATAA ACCTATTACT
    ACAGATGATG AGAGTTCACT GGTGGACCCT GATGACATCA TGAGGCACCG GGGTGACGAC
    GGATCAAACT CTGTAGCCAC TGAGCCCTGG CTCCGCCCAG GCACCTCAGA AACACTGAAG
    CGATTCATGG TGGAACAGCT GAGGCAGGAG CAGCAGCAGG GTCCTGGCAA ACCAGGCGCT
    TTCACCTGGC AGGGACTGTC TACTGCACAC GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50843
    Clone ID Related Accession (Same CDS sequence) XM_005215481.2 , XM_005215481.3
    Accession Version XM_005215481.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005215481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGAAGAATC AAAGAAGTAA AAAACCTATC AGTCAGGTTA AACCTGATTT ACCTTCACAA
    ATACAATCAT CTTTCGAAAA TTCAGAGGGT CCTAGAAAAG ATGTCTTAAC TCCTTCAGAG
    GCATATGAAG AACTTCTAAA CCAAAGACGA CTAGAAGAGG ACAGGTACCG AAAATTAGAT
    GATGAAATTG AATTAAGGAA CAATAGAAGA GTCATTAAAA AGACTGATGA AGAAGTGGGC
    GTTTCCAGTA AAAAACATCA AAGGTTTGAC AGCACGACTG ACATTCCAGA TAGAAGATTT
    CACAGGTTTA ATGAGGATCT TGCTTTTGAT AAACAGTCTT ACAGACCAGG CTATGATCCA
    GAAGTAAGTG AAGACATGGA TGAGAGGTTT AGATATGAAA GTGATTTTGA TAGAAGACTT
    TTGAGAGTTT ATACAAAAAA CAGGATGTAC GGGAACAGAC GAGGCAATGA GCCTCCTATG
    GAGTGTGATG GTGATGTCAC AGAACAGTCA AACATACGAA TTTCATCTGC TGGAAATCAA
    AGTGCTCGAG ACAATGAACC ATCCAAACCT ACTTATCGAG AGACATGCAG TCCTTTTGCG
    GGAATGCTCT TTGGGGGTGA AGATCGAGAA CTTATTCAGA GAAGGAAAGA GAAATATAGA
    CAAGAACTAT TGGAACAAAT GGCTGAACAA CAGAGGAACA AGAGACGAGA AAAAGATTTG
    GATCTCAGGG TTGCAGCTTC TGGAGCACAA GACCCTGAGA AATCGCCTGA TAGACTAAAG
    CAGTTTAGTG TGGCACCAAG ACACTTTGAA GAGATGATAC CACCTGAGAG ACCCAGAATA
    GCTTTCCAGA CACCTCTCCC TCCTCTGTCC GCCCCATCTG TCCCACCCAT CCCATCAGTT
    TACTCCATCC CATCTCAGAG TGAAGATGTG CACAGTGGAC TCAGCAGCAC TCTTGGTGAC
    ATGGTGCCTC CCAGAGTTGC ACCTCTGCCT CCACCTCCCA TGCTGCCCCC TTTGGCTGCG
    AACTACCGAA CTCCTTACGA TGACGCGTAC TATTTCTATG GGGCCAGGAA TACTTTGGAT
    CCTAGTCTTG CTTATTATGG TTCAGGGATG ATGGGAGTAC AGCCTGCTCC TGCTCCTTAT
    GTTACAGCTC CTGTCACCCA CCAGCCAGTA CAGCCTATTG TGAGTACAGT TGGACAGAAT
    GAGCTGAAAA TTACAAGTAA TCGAGCAATA AATTCAGGAT TGTTTTTTGA AGATAAACCA
    AAGCCTTCAA AACAGTCATT TCAGTCTTAC CAGGAAGCTT TGCAGCAGCA GATTCAAGAA
    AGACAAGAAA GAAGAAAGAA AGAACGTGAA GAAAAAGAAG TATATGAAGC CAAATTAGAA
    GCTGAAATGA GAAGTTACAA CCCCTGGGGG AAAGGTGGAG GTGGTGCTCC TCTGAGAGAT
    GACAAAGGAA ATCTGATAAC TGATTTGAAT AGGATGCACA GACAAAATAT AGATGCCTAC
    CATAACCCAG ATGCAAGAGC ATATGAAGAT AAAAGGGCTG TTGTATCTCT AGACCAAAAT
    TTAGCCACTT CAAATGCGGA GAACCAAGAA GATGCTGCAA ATAAAAACTC AGGTCATATG
    CAATCACAGA GCTCTCCTTT TGCTCGGGGA AATATTTTTA GTGAGCCTCC AACTGAACTT
    CAAATTAAAC AGCAGGAATT ATACAAGAAT TTTCTTCGTT TTCAGATTGA GGAAAAGAAG
    CAAAGAGAAG AAGCAGAGCG AGAGAGATTA AGAATTGCTG AAGAAAAAGA AGAAAAACGG
    CTTGCAGAAC AGAGAGCTCG AATCCAGCAA GAGTATGAAG AGGAGCAGGA AAAGAAAAGG
    GAGAAAGAGG AGGAGCAAAG ACTGAAAAAC GAAGAGCTTA TTCGGTTAGC TGAAGAAAGA
    CGAAAAGAAG CAGAAAGAAA GAAGAAAGAA GAAGAAGAAA AACATAATCT ACAACTTCAG
    CACTACTATG AAAGAGAAAA CATGATTGGG GAAGATACAA AGCACTTGAG ACAGCCTTCT
    CCTGTGGTTC CTGCTCTTCA GAATAAGATT GCAAGCAGAC TCCAAAGACC TCCTTCAGTT
    GACAGCATTA TAACTTCCTT TACTCAGGAC AGTTCCATGT CCATGGCACA ATCTCCTCCG
    GTACCTGCCA GGAAAAACCA GCTTCGTGCA GAAGAGGAGA AAAAAAATGT AATTATGGAA
    TTATCAGAAA TGAGAAAACA GCTTCGTAGT GAAGAGCGAC GTCTGCAGGG GCGGTTACTA
    CACATTGATA GTGACGACGA CATTCATATA AGGAAAAGAG AAAGTTCCAT GGATGTATTT
    GATATGGCCA GACATCGATT ACAAGCTCCT GTCAGAAGAC CGTCACCTAA GAGCTTAGAC
    TCTGCTTCTT CTCAGAATAT TCATGACTTT AAGGAGCTGA AAGATAGAGA TTCAGAAACG
    CGAGCTGATC TGAAATCTAT GTACCCAGAT GATCCAAGAG ATAATGACAC CTTGGAGATA
    GAACAACAAG CCTTGCTAAG AGAGCAGCAG AAGAGACTGA ATAAACTAAA AATGCAGGAA
    GTTGACTTGG ATGCTGTCCC AAGCACTAAA GTACGGGACC ACAGAATGCC CAGAGAGGAC
    ACTGATGATT TCTTGAAAAA CTCATTATTA GAATCTGATA GTGCTTTTAT TGGTGCGCAT
    GGCGAGACGT ACCCAGCCAT TGAGGATGGG GGCTTCCTGC TGCTATCTCC GCTGCCCTCC
    GCCAGAGAGC GCAGAAGGAA CAAACTGAAA GGATTGGACT TTGATAACAG TCACCCTGAT
    GTGCCACCAG ATGGTGTCTC TTTAAAATCT GTATCCAGTA TAAATATTGA TCAGCTTAGA
    ATGAGAAATG AAGAACGAAT GCGAAGACTG AATGAACTTC AGAATAAACC TATTACTACA
    GATGATGAGA GTTCACTGGT GGACCCTGAT GACATCATGA GGCACCGGGG TGACGACGGA
    TCAAACTCTG TAGCCACTGA GCCCTGGCTC CGCCCAGGCA CCTCAGAAAC ACTGAAGCGA
    TTCATGGTGG AACAGCTGAG GCAGGAGCAG CAGCAGGGTC CTGGCAAACC AGGCGCTTTC
    ACCTGGCAGG GACTGTCTAC TGCACACGGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215538.2
    CDS133..3765
    Translation

    Target ORF information:

    RefSeq Version XM_005215481.2
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGAAGAATC AAAGAAGTAA AAAACCTATC AGTCAGGTTA AACCTGATTT ACCTTCACAA
    ATACAATCAT CTTTCGAAAA TTCAGAGGGT CCTAGAAAAG ATGTCTTAAC TCCTTCAGAG
    GCATATGAAG AACTTCTAAA CCAAAGACGA CTAGAAGAGG ACAGGTACCG AAAATTAGAT
    GATGAAATTG AATTAAGGAA CAATAGAAGA GTCATTAAAA AGACTGATGA AGAAGTGGGC
    GTTTCCAGTA AAAAACATCA AAGGTTTGAC AGCACGACTG ACATTCCAGA TAGAAGATTT
    CACAGGTTTA ATGAGGATCT TGCTTTTGAT AAACAGTCTT ACAGACCAGG CTATGATCCA
    GAAGTAAGTG AAGACATGGA TGAGAGGTTT AGATATGAAA GTGATTTTGA TAGAAGACTT
    TTGAGAGTTT ATACAAAAAA CAGGATGTAC GGGAACAGAC GAGGCAATGA GCCTCCTATG
    GAGTGTGATG GTGATGTCAC AGAACAGTCA AACATACGAA TTTCATCTGC TGGAAATCAA
    AGTGCTCGAG ACAATGAACC ATCCAAACCT ACTTATCGAG AGACATGCAG TCCTTTTGCG
    GGAATGCTCT TTGGGGGTGA AGATCGAGAA CTTATTCAGA GAAGGAAAGA GAAATATAGA
    CAAGAACTAT TGGAACAAAT GGCTGAACAA CAGAGGAACA AGAGACGAGA AAAAGATTTG
    GATCTCAGGG TTGCAGCTTC TGGAGCACAA GACCCTGAGA AATCGCCTGA TAGACTAAAG
    CAGTTTAGTG TGGCACCAAG ACACTTTGAA GAGATGATAC CACCTGAGAG ACCCAGAATA
    GCTTTCCAGA CACCTCTCCC TCCTCTGTCC GCCCCATCTG TCCCACCCAT CCCATCAGTT
    TACTCCATCC CATCTCAGAG TGAAGATGTG CACAGTGGAC TCAGCAGCAC TCTTGGTGAC
    ATGGTGCCTC CCAGAGTTGC ACCTCTGCCT CCACCTCCCA TGCTGCCCCC TTTGGCTGCG
    AACTACCGAA CTCCTTACGA TGACGCGTAC TATTTCTATG GGGCCAGGAA TACTTTGGAT
    CCTAGTCTTG CTTATTATGG TTCAGGGATG ATGGGAGTAC AGCCTGCTCC TGCTCCTTAT
    GTTACAGCTC CTGTCACCCA CCAGCCAGTA CAGCCTATTG TGAGTACAGT TGGACAGAAT
    GAGCTGAAAA TTACAAGTAA TCGAGCAATA AATTCAGGAT TGTTTTTTGA AGATAAACCA
    AAGCCTTCAA AACAGTCATT TCAGTCTTAC CAGGAAGCTT TGCAGCAGCA GATTCAAGAA
    AGACAAGAAA GAAGAAAGAA AGAACGTGAA GAAAAAGAAG TATATGAAGC CAAATTAGAA
    GCTGAAATGA GAAGTTACAA CCCCTGGGGG AAAGGTGGAG GTGGTGCTCC TCTGAGAGAT
    GACAAAGGAA ATCTGATAAC TGATTTGAAT AGGATGCACA GACAAAATAT AGATGCCTAC
    CATAACCCAG ATGCAAGAGC ATATGAAGAT AAAAGGGCTG TTGTATCTCT AGACCAAAAT
    TTAGCCACTT CAAATGCGGA GAACCAAGAA GATGCTGCAA ATAAAAACTC AGGTCATATG
    CAATCACAGA GCTCTCCTTT TGCTCGGGGA AATATTTTTA GTGAGCCTCC AACTGAACTT
    CAAATTAAAC AGCAGGAATT ATACAAGAAT TTTCTTCGTT TTCAGATTGA GGAAAAGAAG
    CAAAGAGAAG AAGCAGAGCG AGAGAGATTA AGAATTGCTG AAGAAAAAGA AGAAAAACGG
    CTTGCAGAAC AGAGAGCTCG AATCCAGCAA GAGTATGAAG AGGAGCAGGA AAAGAAAAGG
    GAGAAAGAGG AGGAGCAAAG ACTGAAAAAC GAAGAGCTTA TTCGGTTAGC TGAAGAAAGA
    CGAAAAGAAG CAGAAAGAAA GAAGAAAGAA GAAGAAGAAA AACATAATCT ACAACTTCAG
    CACTACTATG AAAGAGAAAA CATGATTGGG GAAGATACAA AGCACTTGAG ACAGCCTTCT
    CCTGTGGTTC CTGCTCTTCA GAATAAGATT GCAAGCAGAC TCCAAAGACC TCCTTCAGTT
    GACAGCATTA TAACTTCCTT TACTCAGGAC AGTTCCATGT CCATGGCACA ATCTCCTCCG
    GTACCTGCCA GGAAAAACCA GCTTCGTGCA GAAGAGGAGA AAAAAAATGT AATTATGGAA
    TTATCAGAAA TGAGAAAACA GCTTCGTAGT GAAGAGCGAC GTCTGCAGGG GCGGTTACTA
    CACATTGATA GTGACGACGA CATTCATATA AGGAAAAGAG AAAGTTCCAT GGATGTATTT
    GATATGGCCA GACATCGATT ACAAGCTCCT GTCAGAAGAC CGTCACCTAA GAGCTTAGAC
    TCTGCTTCTT CTCAGAATAT TCATGACTTT AAGGAGCTGA AAGATAGAGA TTCAGAAACG
    CGAGCTGATC TGAAATCTAT GTACCCAGAT GATCCAAGAG ATAATGACAC CTTGGAGATA
    GAACAACAAG CCTTGCTAAG AGAGCAGCAG AAGAGACTGA ATAAACTAAA AATGCAGGAA
    GTTGACTTGG ATGCTGTCCC AAGCACTAAA GTACGGGACC ACAGAATGCC CAGAGAGGAC
    ACTGATGATT TCTTGAAAAA CTCATTATTA GAATCTGATA GTGCTTTTAT TGGTGCGCAT
    GGCGAGACGT ACCCAGCCAT TGAGGATGGG GGCTTCCTGC TGCTATCTCC GCTGCCCTCC
    GCCAGAGAGC GCAGAAGGAA CAAACTGAAA GGATTGGACT TTGATAACAG TCACCCTGAT
    GTGCCACCAG ATGGTGTCTC TTTAAAATCT GTATCCAGTA TAAATATTGA TCAGCTTAGA
    ATGAGAAATG AAGAACGAAT GCGAAGACTG AATGAACTTC AGAATAAACC TATTACTACA
    GATGATGAGA GTTCACTGGT GGACCCTGAT GACATCATGA GGCACCGGGG TGACGACGGA
    TCAAACTCTG TAGCCACTGA GCCCTGGCTC CGCCCAGGCA CCTCAGAAAC ACTGAAGCGA
    TTCATGGTGG AACAGCTGAG GCAGGAGCAG CAGCAGGGTC CTGGCAAACC AGGCGCTTTC
    ACCTGGCAGG GACTGTCTAC TGCACACGGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50843
    Clone ID Related Accession (Same CDS sequence) XM_005215481.2 , XM_005215481.3
    Accession Version XM_005215481.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215481.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGAAGAATC AAAGAAGTAA AAAACCTATC AGTCAGGTTA AACCTGATTT ACCTTCACAA
    ATACAATCAT CTTTCGAAAA TTCAGAGGGT CCTAGAAAAG ATGTCTTAAC TCCTTCAGAG
    GCATATGAAG AACTTCTAAA CCAAAGACGA CTAGAAGAGG ACAGGTACCG AAAATTAGAT
    GATGAAATTG AATTAAGGAA CAATAGAAGA GTCATTAAAA AGACTGATGA AGAAGTGGGC
    GTTTCCAGTA AAAAACATCA AAGGTTTGAC AGCACGACTG ACATTCCAGA TAGAAGATTT
    CACAGGTTTA ATGAGGATCT TGCTTTTGAT AAACAGTCTT ACAGACCAGG CTATGATCCA
    GAAGTAAGTG AAGACATGGA TGAGAGGTTT AGATATGAAA GTGATTTTGA TAGAAGACTT
    TTGAGAGTTT ATACAAAAAA CAGGATGTAC GGGAACAGAC GAGGCAATGA GCCTCCTATG
    GAGTGTGATG GTGATGTCAC AGAACAGTCA AACATACGAA TTTCATCTGC TGGAAATCAA
    AGTGCTCGAG ACAATGAACC ATCCAAACCT ACTTATCGAG AGACATGCAG TCCTTTTGCG
    GGAATGCTCT TTGGGGGTGA AGATCGAGAA CTTATTCAGA GAAGGAAAGA GAAATATAGA
    CAAGAACTAT TGGAACAAAT GGCTGAACAA CAGAGGAACA AGAGACGAGA AAAAGATTTG
    GATCTCAGGG TTGCAGCTTC TGGAGCACAA GACCCTGAGA AATCGCCTGA TAGACTAAAG
    CAGTTTAGTG TGGCACCAAG ACACTTTGAA GAGATGATAC CACCTGAGAG ACCCAGAATA
    GCTTTCCAGA CACCTCTCCC TCCTCTGTCC GCCCCATCTG TCCCACCCAT CCCATCAGTT
    TACTCCATCC CATCTCAGAG TGAAGATGTG CACAGTGGAC TCAGCAGCAC TCTTGGTGAC
    ATGGTGCCTC CCAGAGTTGC ACCTCTGCCT CCACCTCCCA TGCTGCCCCC TTTGGCTGCG
    AACTACCGAA CTCCTTACGA TGACGCGTAC TATTTCTATG GGGCCAGGAA TACTTTGGAT
    CCTAGTCTTG CTTATTATGG TTCAGGGATG ATGGGAGTAC AGCCTGCTCC TGCTCCTTAT
    GTTACAGCTC CTGTCACCCA CCAGCCAGTA CAGCCTATTG TGAGTACAGT TGGACAGAAT
    GAGCTGAAAA TTACAAGTAA TCGAGCAATA AATTCAGGAT TGTTTTTTGA AGATAAACCA
    AAGCCTTCAA AACAGTCATT TCAGTCTTAC CAGGAAGCTT TGCAGCAGCA GATTCAAGAA
    AGACAAGAAA GAAGAAAGAA AGAACGTGAA GAAAAAGAAG TATATGAAGC CAAATTAGAA
    GCTGAAATGA GAAGTTACAA CCCCTGGGGG AAAGGTGGAG GTGGTGCTCC TCTGAGAGAT
    GACAAAGGAA ATCTGATAAC TGATTTGAAT AGGATGCACA GACAAAATAT AGATGCCTAC
    CATAACCCAG ATGCAAGAGC ATATGAAGAT AAAAGGGCTG TTGTATCTCT AGACCAAAAT
    TTAGCCACTT CAAATGCGGA GAACCAAGAA GATGCTGCAA ATAAAAACTC AGGTCATATG
    CAATCACAGA GCTCTCCTTT TGCTCGGGGA AATATTTTTA GTGAGCCTCC AACTGAACTT
    CAAATTAAAC AGCAGGAATT ATACAAGAAT TTTCTTCGTT TTCAGATTGA GGAAAAGAAG
    CAAAGAGAAG AAGCAGAGCG AGAGAGATTA AGAATTGCTG AAGAAAAAGA AGAAAAACGG
    CTTGCAGAAC AGAGAGCTCG AATCCAGCAA GAGTATGAAG AGGAGCAGGA AAAGAAAAGG
    GAGAAAGAGG AGGAGCAAAG ACTGAAAAAC GAAGAGCTTA TTCGGTTAGC TGAAGAAAGA
    CGAAAAGAAG CAGAAAGAAA GAAGAAAGAA GAAGAAGAAA AACATAATCT ACAACTTCAG
    CACTACTATG AAAGAGAAAA CATGATTGGG GAAGATACAA AGCACTTGAG ACAGCCTTCT
    CCTGTGGTTC CTGCTCTTCA GAATAAGATT GCAAGCAGAC TCCAAAGACC TCCTTCAGTT
    GACAGCATTA TAACTTCCTT TACTCAGGAC AGTTCCATGT CCATGGCACA ATCTCCTCCG
    GTACCTGCCA GGAAAAACCA GCTTCGTGCA GAAGAGGAGA AAAAAAATGT AATTATGGAA
    TTATCAGAAA TGAGAAAACA GCTTCGTAGT GAAGAGCGAC GTCTGCAGGG GCGGTTACTA
    CACATTGATA GTGACGACGA CATTCATATA AGGAAAAGAG AAAGTTCCAT GGATGTATTT
    GATATGGCCA GACATCGATT ACAAGCTCCT GTCAGAAGAC CGTCACCTAA GAGCTTAGAC
    TCTGCTTCTT CTCAGAATAT TCATGACTTT AAGGAGCTGA AAGATAGAGA TTCAGAAACG
    CGAGCTGATC TGAAATCTAT GTACCCAGAT GATCCAAGAG ATAATGACAC CTTGGAGATA
    GAACAACAAG CCTTGCTAAG AGAGCAGCAG AAGAGACTGA ATAAACTAAA AATGCAGGAA
    GTTGACTTGG ATGCTGTCCC AAGCACTAAA GTACGGGACC ACAGAATGCC CAGAGAGGAC
    ACTGATGATT TCTTGAAAAA CTCATTATTA GAATCTGATA GTGCTTTTAT TGGTGCGCAT
    GGCGAGACGT ACCCAGCCAT TGAGGATGGG GGCTTCCTGC TGCTATCTCC GCTGCCCTCC
    GCCAGAGAGC GCAGAAGGAA CAAACTGAAA GGATTGGACT TTGATAACAG TCACCCTGAT
    GTGCCACCAG ATGGTGTCTC TTTAAAATCT GTATCCAGTA TAAATATTGA TCAGCTTAGA
    ATGAGAAATG AAGAACGAAT GCGAAGACTG AATGAACTTC AGAATAAACC TATTACTACA
    GATGATGAGA GTTCACTGGT GGACCCTGAT GACATCATGA GGCACCGGGG TGACGACGGA
    TCAAACTCTG TAGCCACTGA GCCCTGGCTC CGCCCAGGCA CCTCAGAAAC ACTGAAGCGA
    TTCATGGTGG AACAGCTGAG GCAGGAGCAG CAGCAGGGTC CTGGCAAACC AGGCGCTTTC
    ACCTGGCAGG GACTGTCTAC TGCACACGGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215538.2
    CDS182..3814
    Translation

    Target ORF information:

    RefSeq Version XM_005215481.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215481.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGAAAAATT TTCTATCCAC GAGTGAAACA GATCCATCTA CTCTGGGAGT TTCCCTTCCT
    ATTGGTGAGA GGTTATCTGC TAAGGAAAGG TTGAAACTTG AACGTAACAA AGAATACAAT
    CAATTTCTCA GGGATAAGGA AGAATCCACT GAAAAGTTCA GACAGGTAGA AAAGAGTACT
    GAGAAGAATC AAAGAAGTAA AAAACCTATC AGTCAGGTTA AACCTGATTT ACCTTCACAA
    ATACAATCAT CTTTCGAAAA TTCAGAGGGT CCTAGAAAAG ATGTCTTAAC TCCTTCAGAG
    GCATATGAAG AACTTCTAAA CCAAAGACGA CTAGAAGAGG ACAGGTACCG AAAATTAGAT
    GATGAAATTG AATTAAGGAA CAATAGAAGA GTCATTAAAA AGACTGATGA AGAAGTGGGC
    GTTTCCAGTA AAAAACATCA AAGGTTTGAC AGCACGACTG ACATTCCAGA TAGAAGATTT
    CACAGGTTTA ATGAGGATCT TGCTTTTGAT AAACAGTCTT ACAGACCAGG CTATGATCCA
    GAAGTAAGTG AAGACATGGA TGAGAGGTTT AGATATGAAA GTGATTTTGA TAGAAGACTT
    TTGAGAGTTT ATACAAAAAA CAGGATGTAC GGGAACAGAC GAGGCAATGA GCCTCCTATG
    GAGTGTGATG GTGATGTCAC AGAACAGTCA AACATACGAA TTTCATCTGC TGGAAATCAA
    AGTGCTCGAG ACAATGAACC ATCCAAACCT ACTTATCGAG AGACATGCAG TCCTTTTGCG
    GGAATGCTCT TTGGGGGTGA AGATCGAGAA CTTATTCAGA GAAGGAAAGA GAAATATAGA
    CAAGAACTAT TGGAACAAAT GGCTGAACAA CAGAGGAACA AGAGACGAGA AAAAGATTTG
    GATCTCAGGG TTGCAGCTTC TGGAGCACAA GACCCTGAGA AATCGCCTGA TAGACTAAAG
    CAGTTTAGTG TGGCACCAAG ACACTTTGAA GAGATGATAC CACCTGAGAG ACCCAGAATA
    GCTTTCCAGA CACCTCTCCC TCCTCTGTCC GCCCCATCTG TCCCACCCAT CCCATCAGTT
    TACTCCATCC CATCTCAGAG TGAAGATGTG CACAGTGGAC TCAGCAGCAC TCTTGGTGAC
    ATGGTGCCTC CCAGAGTTGC ACCTCTGCCT CCACCTCCCA TGCTGCCCCC TTTGGCTGCG
    AACTACCGAA CTCCTTACGA TGACGCGTAC TATTTCTATG GGGCCAGGAA TACTTTGGAT
    CCTAGTCTTG CTTATTATGG TTCAGGGATG ATGGGAGTAC AGCCTGCTCC TGCTCCTTAT
    GTTACAGCTC CTGTCACCCA CCAGCCAGTA CAGCCTATTG TGAGTACAGT TGGACAGAAT
    GAGCTGAAAA TTACAAGTAA TCGAGCAATA AATTCAGGAT TGTTTTTTGA AGATAAACCA
    AAGCCTTCAA AACAGTCATT TCAGTCTTAC CAGGAAGCTT TGCAGCAGCA GATTCAAGAA
    AGACAAGAAA GAAGAAAGAA AGAACGTGAA GAAAAAGAAG TATATGAAGC CAAATTAGAA
    GCTGAAATGA GAAGTTACAA CCCCTGGGGG AAAGGTGGAG GTGGTGCTCC TCTGAGAGAT
    GACAAAGGAA ATCTGATAAC TGATTTGAAT AGGATGCACA GACAAAATAT AGATGCCTAC
    CATAACCCAG ATGCAAGAGC ATATGAAGAT AAAAGGGCTG TTGTATCTCT AGACCAAAAT
    TTAGCCACTT CAAATGCGGA GAACCAAGAA GATGCTGCAA ATAAAAACTC AGGTCATATG
    CAATCACAGA GCTCTCCTTT TGCTCGGGGA AATATTTTTA GTGAGCCTCC AACTGAACTT
    CAAATTAAAC AGCAGGAATT ATACAAGAAT TTTCTTCGTT TTCAGATTGA GGAAAAGAAG
    CAAAGAGAAG AAGCAGAGCG AGAGAGATTA AGAATTGCTG AAGAAAAAGA AGAAAAACGG
    CTTGCAGAAC AGAGAGCTCG AATCCAGCAA GAGTATGAAG AGGAGCAGGA AAAGAAAAGG
    GAGAAAGAGG AGGAGCAAAG ACTGAAAAAC GAAGAGCTTA TTCGGTTAGC TGAAGAAAGA
    CGAAAAGAAG CAGAAAGAAA GAAGAAAGAA GAAGAAGAAA AACATAATCT ACAACTTCAG
    CACTACTATG AAAGAGAAAA CATGATTGGG GAAGATACAA AGCACTTGAG ACAGCCTTCT
    CCTGTGGTTC CTGCTCTTCA GAATAAGATT GCAAGCAGAC TCCAAAGACC TCCTTCAGTT
    GACAGCATTA TAACTTCCTT TACTCAGGAC AGTTCCATGT CCATGGCACA ATCTCCTCCG
    GTACCTGCCA GGAAAAACCA GCTTCGTGCA GAAGAGGAGA AAAAAAATGT AATTATGGAA
    TTATCAGAAA TGAGAAAACA GCTTCGTAGT GAAGAGCGAC GTCTGCAGGG GCGGTTACTA
    CACATTGATA GTGACGACGA CATTCATATA AGGAAAAGAG AAAGTTCCAT GGATGTATTT
    GATATGGCCA GACATCGATT ACAAGCTCCT GTCAGAAGAC CGTCACCTAA GAGCTTAGAC
    TCTGCTTCTT CTCAGAATAT TCATGACTTT AAGGAGCTGA AAGATAGAGA TTCAGAAACG
    CGAGCTGATC TGAAATCTAT GTACCCAGAT GATCCAAGAG ATAATGACAC CTTGGAGATA
    GAACAACAAG CCTTGCTAAG AGAGCAGCAG AAGAGACTGA ATAAACTAAA AATGCAGGAA
    GTTGACTTGG ATGCTGTCCC AAGCACTAAA GTACGGGACC ACAGAATGCC CAGAGAGGAC
    ACTGATGATT TCTTGAAAAA CTCATTATTA GAATCTGATA GTGCTTTTAT TGGTGCGCAT
    GGCGAGACGT ACCCAGCCAT TGAGGATGGG GGCTTCCTGC TGCTATCTCC GCTGCCCTCC
    GCCAGAGAGC GCAGAAGGAA CAAACTGAAA GGATTGGACT TTGATAACAG TCACCCTGAT
    GTGCCACCAG ATGGTGTCTC TTTAAAATCT GTATCCAGTA TAAATATTGA TCAGCTTAGA
    ATGAGAAATG AAGAACGAAT GCGAAGACTG AATGAACTTC AGAATAAACC TATTACTACA
    GATGATGAGA GTTCACTGGT GGACCCTGAT GACATCATGA GGCACCGGGG TGACGACGGA
    TCAAACTCTG TAGCCACTGA GCCCTGGCTC CGCCCAGGCA CCTCAGAAAC ACTGAAGCGA
    TTCATGGTGG AACAGCTGAG GCAGGAGCAG CAGCAGGGTC CTGGCAAACC AGGCGCTTTC
    ACCTGGCAGG GACTGTCTAC TGCACACGGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50844
    Clone ID Related Accession (Same CDS sequence) XM_005215482.3 , XM_005215482.4
    Accession Version XM_005215482.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215482.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG TGCTCGAGAC
    AATGAACCAT CCAAACCTAC TTATCGAGAG ACATGCAGTC CTTTTGCGGG AATGCTCTTT
    GGGGGTGAAG ATCGAGAACT TATTCAGAGA AGGAAAGAGA AATATAGACA AGAACTATTG
    GAACAAATGG CTGAACAACA GAGGAACAAG AGACGAGAAA AAGATTTGGA TCTCAGGGTT
    GCAGCTTCTG GAGCACAAGA CCCTGAGAAA TCGCCTGATA GACTAAAGCA GTTTAGTGTG
    GCACCAAGAC ACTTTGAAGA GATGATACCA CCTGAGAGAC CCAGAATAGC TTTCCAGACA
    CCTCTCCCTC CTCTGTCCGC CCCATCTGTC CCACCCATCC CATCAGTTTA CTCCATCCCA
    TCTCAGAGTG AAGATGTGCA CAGTGGACTC AGCAGCACTC TTGGTGACAT GGTGCCTCCC
    AGAGTTGCAC CTCTGCCTCC ACCTCCCATG CTGCCCCCTT TGGCTGCGAA CTACCGAACT
    CCTTACGATG ACGCGTACTA TTTCTATGGG GCCAGGAATA CTTTGGATCC TAGTCTTGCT
    TATTATGGTT CAGGGATGAT GGGAGTACAG CCTGCTCCTG CTCCTTATGT TACAGCTCCT
    GTCACCCACC AGCCAGTACA GCCTATTGTG AGTACAGTTG GACAGAATGA GCTGAAAATT
    ACAAGTAATC GAGCAATAAA TTCAGGATTG TTTTTTGAAG ATAAACCAAA GCCTTCAAAA
    CAGTCATTTC AGTCTTACCA GGAAGCTTTG CAGCAGCAGA TTCAAGAAAG ACAAGAAAGA
    AGAAAGAAAG AACGTGAAGA AAAAGAAGTA TATGAAGCCA AATTAGAAGC TGAAATGAGA
    AGTTACAACC CCTGGGGGAA AGGTGGAGGT GGTGCTCCTC TGAGAGATGA CAAAGGAAAT
    CTGATAACTG ATTTGAATAG GATGCACAGA CAAAATATAG ATGCCTACCA TAACCCAGAT
    GCAAGAGCAT ATGAAGATAA AAGGGCTGTT GTATCTCTAG ACCAAAATTT AGCCACTTCA
    AATGCGGAGA ACCAAGAAGA TGCTGCAAAT AAAAACTCAG GTCATATGCA ATCACAGAGC
    TCTCCTTTTG CTCGGGGAAA TATTTTTAGT GAGCCTCCAA CTGAACTTCA AATTAAACAG
    CAGGAATTAT ACAAGAATTT TCTTCGTTTT CAGATTGAGG AAAAGAAGCA AAGAGAAGAA
    GCAGAGCGAG AGAGATTAAG AATTGCTGAA GAAAAAGAAG AAAAACGGCT TGCAGAACAG
    AGAGCTCGAA TCCAGCAAGA GTATGAAGAG GAGCAGGAAA AGAAAAGGGA GAAAGAGGAG
    GAGCAAAGAC TGAAAAACGA AGAGCTTATT CGGTTAGCTG AAGAAAGACG AAAAGAAGCA
    GAAAGAAAGA AGAAAGAAGA AGAAGAAAAA CATAATCTAC AACTTCAGCA CTACTATGAA
    AGAGAAAACA TGATTGGGGA AGATACAAAG CACTTGAGAC AGCCTTCTCC TGTGGTTCCT
    GCTCTTCAGA ATAAGATTGC AAGCAGACTC CAAAGACCTC CTTCAGTTGA CAGCATTATA
    ACTTCCTTTA CTCAGGACAG TTCCATGTCC ATGGCACAAT CTCCTCCGGT ACCTGCCAGG
    AAAAACCAGC TTCGTGCAGA AGAGGAGAAA AAAAATGTAA TTATGGAATT ATCAGAAATG
    AGAAAACAGC TTCGTAGTGA AGAGCGACGT CTGCAGGGGC GGTTACTACA CATTGATAGT
    GACGACGACA TTCATATAAG GAAAAGAGAA AGTTCCATGG ATGTATTTGA TATGGCCAGA
    CATCGATTAC AAGCTCCTGT CAGAAGACCG TCACCTAAGA GCTTAGACTC TGCTTCTTCT
    CAGAATATTC ATGACTTTAA GGAGCTGAAA GATAGAGATT CAGAAACGCG AGCTGATCTG
    AAATCTATGT ACCCAGATGA TCCAAGAGAT AATGACACCT TGGAGATAGA ACAACAAGCC
    TTGCTAAGAG AGCAGCAGAA GAGACTGAAT AAACTAAAAA TGCAGGAAGT TGACTTGGAT
    GCTGTCCCAA GCACTAAAGT ACGGGACCAC AGAATGCCCA GAGAGGACAC TGATGATTTC
    TTGAAAAACT CATTATTAGA ATCTGATAGT GCTTTTATTG GTGCGCATGG CGAGACGTAC
    CCAGCCATTG AGGATGGGGG CTTCCTGCTG CTATCTCCGC TGCCCTCCGC CAGAGAGCGC
    AGAAGGAACA AACTGAAAGG ATTGGACTTT GATAACAGTC ACCCTGATGT GCCACCAGAT
    GGTGTCTCTT TAAAATCTGT ATCCAGTATA AATATTGATC AGCTTAGAAT GAGAAATGAA
    GAACGAATGC GAAGACTGAA TGAACTTCAG AATAAACCTA TTACTACAGA TGATGAGAGT
    TCACTGGTGG ACCCTGATGA CATCATGAGG CACCGGGGTG ACGACGGATC AAACTCTGTA
    GCCACTGAGC CCTGGCTCCG CCCAGGCACC TCAGAAACAC TGAAGCGATT CATGGTGGAA
    CAGCTGAGGC AGGAGCAGCA GCAGGGTCCT GGCAAACCAG GCGCTTTCAC CTGGCAGGGA
    CTGTCTACTG CACACGGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215539.2
    CDS114..3674
    Translation

    Target ORF information:

    RefSeq Version XM_005215482.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215482.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG TGCTCGAGAC
    AATGAACCAT CCAAACCTAC TTATCGAGAG ACATGCAGTC CTTTTGCGGG AATGCTCTTT
    GGGGGTGAAG ATCGAGAACT TATTCAGAGA AGGAAAGAGA AATATAGACA AGAACTATTG
    GAACAAATGG CTGAACAACA GAGGAACAAG AGACGAGAAA AAGATTTGGA TCTCAGGGTT
    GCAGCTTCTG GAGCACAAGA CCCTGAGAAA TCGCCTGATA GACTAAAGCA GTTTAGTGTG
    GCACCAAGAC ACTTTGAAGA GATGATACCA CCTGAGAGAC CCAGAATAGC TTTCCAGACA
    CCTCTCCCTC CTCTGTCCGC CCCATCTGTC CCACCCATCC CATCAGTTTA CTCCATCCCA
    TCTCAGAGTG AAGATGTGCA CAGTGGACTC AGCAGCACTC TTGGTGACAT GGTGCCTCCC
    AGAGTTGCAC CTCTGCCTCC ACCTCCCATG CTGCCCCCTT TGGCTGCGAA CTACCGAACT
    CCTTACGATG ACGCGTACTA TTTCTATGGG GCCAGGAATA CTTTGGATCC TAGTCTTGCT
    TATTATGGTT CAGGGATGAT GGGAGTACAG CCTGCTCCTG CTCCTTATGT TACAGCTCCT
    GTCACCCACC AGCCAGTACA GCCTATTGTG AGTACAGTTG GACAGAATGA GCTGAAAATT
    ACAAGTAATC GAGCAATAAA TTCAGGATTG TTTTTTGAAG ATAAACCAAA GCCTTCAAAA
    CAGTCATTTC AGTCTTACCA GGAAGCTTTG CAGCAGCAGA TTCAAGAAAG ACAAGAAAGA
    AGAAAGAAAG AACGTGAAGA AAAAGAAGTA TATGAAGCCA AATTAGAAGC TGAAATGAGA
    AGTTACAACC CCTGGGGGAA AGGTGGAGGT GGTGCTCCTC TGAGAGATGA CAAAGGAAAT
    CTGATAACTG ATTTGAATAG GATGCACAGA CAAAATATAG ATGCCTACCA TAACCCAGAT
    GCAAGAGCAT ATGAAGATAA AAGGGCTGTT GTATCTCTAG ACCAAAATTT AGCCACTTCA
    AATGCGGAGA ACCAAGAAGA TGCTGCAAAT AAAAACTCAG GTCATATGCA ATCACAGAGC
    TCTCCTTTTG CTCGGGGAAA TATTTTTAGT GAGCCTCCAA CTGAACTTCA AATTAAACAG
    CAGGAATTAT ACAAGAATTT TCTTCGTTTT CAGATTGAGG AAAAGAAGCA AAGAGAAGAA
    GCAGAGCGAG AGAGATTAAG AATTGCTGAA GAAAAAGAAG AAAAACGGCT TGCAGAACAG
    AGAGCTCGAA TCCAGCAAGA GTATGAAGAG GAGCAGGAAA AGAAAAGGGA GAAAGAGGAG
    GAGCAAAGAC TGAAAAACGA AGAGCTTATT CGGTTAGCTG AAGAAAGACG AAAAGAAGCA
    GAAAGAAAGA AGAAAGAAGA AGAAGAAAAA CATAATCTAC AACTTCAGCA CTACTATGAA
    AGAGAAAACA TGATTGGGGA AGATACAAAG CACTTGAGAC AGCCTTCTCC TGTGGTTCCT
    GCTCTTCAGA ATAAGATTGC AAGCAGACTC CAAAGACCTC CTTCAGTTGA CAGCATTATA
    ACTTCCTTTA CTCAGGACAG TTCCATGTCC ATGGCACAAT CTCCTCCGGT ACCTGCCAGG
    AAAAACCAGC TTCGTGCAGA AGAGGAGAAA AAAAATGTAA TTATGGAATT ATCAGAAATG
    AGAAAACAGC TTCGTAGTGA AGAGCGACGT CTGCAGGGGC GGTTACTACA CATTGATAGT
    GACGACGACA TTCATATAAG GAAAAGAGAA AGTTCCATGG ATGTATTTGA TATGGCCAGA
    CATCGATTAC AAGCTCCTGT CAGAAGACCG TCACCTAAGA GCTTAGACTC TGCTTCTTCT
    CAGAATATTC ATGACTTTAA GGAGCTGAAA GATAGAGATT CAGAAACGCG AGCTGATCTG
    AAATCTATGT ACCCAGATGA TCCAAGAGAT AATGACACCT TGGAGATAGA ACAACAAGCC
    TTGCTAAGAG AGCAGCAGAA GAGACTGAAT AAACTAAAAA TGCAGGAAGT TGACTTGGAT
    GCTGTCCCAA GCACTAAAGT ACGGGACCAC AGAATGCCCA GAGAGGACAC TGATGATTTC
    TTGAAAAACT CATTATTAGA ATCTGATAGT GCTTTTATTG GTGCGCATGG CGAGACGTAC
    CCAGCCATTG AGGATGGGGG CTTCCTGCTG CTATCTCCGC TGCCCTCCGC CAGAGAGCGC
    AGAAGGAACA AACTGAAAGG ATTGGACTTT GATAACAGTC ACCCTGATGT GCCACCAGAT
    GGTGTCTCTT TAAAATCTGT ATCCAGTATA AATATTGATC AGCTTAGAAT GAGAAATGAA
    GAACGAATGC GAAGACTGAA TGAACTTCAG AATAAACCTA TTACTACAGA TGATGAGAGT
    TCACTGGTGG ACCCTGATGA CATCATGAGG CACCGGGGTG ACGACGGATC AAACTCTGTA
    GCCACTGAGC CCTGGCTCCG CCCAGGCACC TCAGAAACAC TGAAGCGATT CATGGTGGAA
    CAGCTGAGGC AGGAGCAGCA GCAGGGTCCT GGCAAACCAG GCGCTTTCAC CTGGCAGGGA
    CTGTCTACTG CACACGGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50844
    Clone ID Related Accession (Same CDS sequence) XM_005215482.3 , XM_005215482.4
    Accession Version XM_005215482.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215482.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG TGCTCGAGAC
    AATGAACCAT CCAAACCTAC TTATCGAGAG ACATGCAGTC CTTTTGCGGG AATGCTCTTT
    GGGGGTGAAG ATCGAGAACT TATTCAGAGA AGGAAAGAGA AATATAGACA AGAACTATTG
    GAACAAATGG CTGAACAACA GAGGAACAAG AGACGAGAAA AAGATTTGGA TCTCAGGGTT
    GCAGCTTCTG GAGCACAAGA CCCTGAGAAA TCGCCTGATA GACTAAAGCA GTTTAGTGTG
    GCACCAAGAC ACTTTGAAGA GATGATACCA CCTGAGAGAC CCAGAATAGC TTTCCAGACA
    CCTCTCCCTC CTCTGTCCGC CCCATCTGTC CCACCCATCC CATCAGTTTA CTCCATCCCA
    TCTCAGAGTG AAGATGTGCA CAGTGGACTC AGCAGCACTC TTGGTGACAT GGTGCCTCCC
    AGAGTTGCAC CTCTGCCTCC ACCTCCCATG CTGCCCCCTT TGGCTGCGAA CTACCGAACT
    CCTTACGATG ACGCGTACTA TTTCTATGGG GCCAGGAATA CTTTGGATCC TAGTCTTGCT
    TATTATGGTT CAGGGATGAT GGGAGTACAG CCTGCTCCTG CTCCTTATGT TACAGCTCCT
    GTCACCCACC AGCCAGTACA GCCTATTGTG AGTACAGTTG GACAGAATGA GCTGAAAATT
    ACAAGTAATC GAGCAATAAA TTCAGGATTG TTTTTTGAAG ATAAACCAAA GCCTTCAAAA
    CAGTCATTTC AGTCTTACCA GGAAGCTTTG CAGCAGCAGA TTCAAGAAAG ACAAGAAAGA
    AGAAAGAAAG AACGTGAAGA AAAAGAAGTA TATGAAGCCA AATTAGAAGC TGAAATGAGA
    AGTTACAACC CCTGGGGGAA AGGTGGAGGT GGTGCTCCTC TGAGAGATGA CAAAGGAAAT
    CTGATAACTG ATTTGAATAG GATGCACAGA CAAAATATAG ATGCCTACCA TAACCCAGAT
    GCAAGAGCAT ATGAAGATAA AAGGGCTGTT GTATCTCTAG ACCAAAATTT AGCCACTTCA
    AATGCGGAGA ACCAAGAAGA TGCTGCAAAT AAAAACTCAG GTCATATGCA ATCACAGAGC
    TCTCCTTTTG CTCGGGGAAA TATTTTTAGT GAGCCTCCAA CTGAACTTCA AATTAAACAG
    CAGGAATTAT ACAAGAATTT TCTTCGTTTT CAGATTGAGG AAAAGAAGCA AAGAGAAGAA
    GCAGAGCGAG AGAGATTAAG AATTGCTGAA GAAAAAGAAG AAAAACGGCT TGCAGAACAG
    AGAGCTCGAA TCCAGCAAGA GTATGAAGAG GAGCAGGAAA AGAAAAGGGA GAAAGAGGAG
    GAGCAAAGAC TGAAAAACGA AGAGCTTATT CGGTTAGCTG AAGAAAGACG AAAAGAAGCA
    GAAAGAAAGA AGAAAGAAGA AGAAGAAAAA CATAATCTAC AACTTCAGCA CTACTATGAA
    AGAGAAAACA TGATTGGGGA AGATACAAAG CACTTGAGAC AGCCTTCTCC TGTGGTTCCT
    GCTCTTCAGA ATAAGATTGC AAGCAGACTC CAAAGACCTC CTTCAGTTGA CAGCATTATA
    ACTTCCTTTA CTCAGGACAG TTCCATGTCC ATGGCACAAT CTCCTCCGGT ACCTGCCAGG
    AAAAACCAGC TTCGTGCAGA AGAGGAGAAA AAAAATGTAA TTATGGAATT ATCAGAAATG
    AGAAAACAGC TTCGTAGTGA AGAGCGACGT CTGCAGGGGC GGTTACTACA CATTGATAGT
    GACGACGACA TTCATATAAG GAAAAGAGAA AGTTCCATGG ATGTATTTGA TATGGCCAGA
    CATCGATTAC AAGCTCCTGT CAGAAGACCG TCACCTAAGA GCTTAGACTC TGCTTCTTCT
    CAGAATATTC ATGACTTTAA GGAGCTGAAA GATAGAGATT CAGAAACGCG AGCTGATCTG
    AAATCTATGT ACCCAGATGA TCCAAGAGAT AATGACACCT TGGAGATAGA ACAACAAGCC
    TTGCTAAGAG AGCAGCAGAA GAGACTGAAT AAACTAAAAA TGCAGGAAGT TGACTTGGAT
    GCTGTCCCAA GCACTAAAGT ACGGGACCAC AGAATGCCCA GAGAGGACAC TGATGATTTC
    TTGAAAAACT CATTATTAGA ATCTGATAGT GCTTTTATTG GTGCGCATGG CGAGACGTAC
    CCAGCCATTG AGGATGGGGG CTTCCTGCTG CTATCTCCGC TGCCCTCCGC CAGAGAGCGC
    AGAAGGAACA AACTGAAAGG ATTGGACTTT GATAACAGTC ACCCTGATGT GCCACCAGAT
    GGTGTCTCTT TAAAATCTGT ATCCAGTATA AATATTGATC AGCTTAGAAT GAGAAATGAA
    GAACGAATGC GAAGACTGAA TGAACTTCAG AATAAACCTA TTACTACAGA TGATGAGAGT
    TCACTGGTGG ACCCTGATGA CATCATGAGG CACCGGGGTG ACGACGGATC AAACTCTGTA
    GCCACTGAGC CCTGGCTCCG CCCAGGCACC TCAGAAACAC TGAAGCGATT CATGGTGGAA
    CAGCTGAGGC AGGAGCAGCA GCAGGGTCCT GGCAAACCAG GCGCTTTCAC CTGGCAGGGA
    CTGTCTACTG CACACGGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215539.2
    CDS182..3742
    Translation

    Target ORF information:

    RefSeq Version XM_005215482.4
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215482.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG TGCTCGAGAC
    AATGAACCAT CCAAACCTAC TTATCGAGAG ACATGCAGTC CTTTTGCGGG AATGCTCTTT
    GGGGGTGAAG ATCGAGAACT TATTCAGAGA AGGAAAGAGA AATATAGACA AGAACTATTG
    GAACAAATGG CTGAACAACA GAGGAACAAG AGACGAGAAA AAGATTTGGA TCTCAGGGTT
    GCAGCTTCTG GAGCACAAGA CCCTGAGAAA TCGCCTGATA GACTAAAGCA GTTTAGTGTG
    GCACCAAGAC ACTTTGAAGA GATGATACCA CCTGAGAGAC CCAGAATAGC TTTCCAGACA
    CCTCTCCCTC CTCTGTCCGC CCCATCTGTC CCACCCATCC CATCAGTTTA CTCCATCCCA
    TCTCAGAGTG AAGATGTGCA CAGTGGACTC AGCAGCACTC TTGGTGACAT GGTGCCTCCC
    AGAGTTGCAC CTCTGCCTCC ACCTCCCATG CTGCCCCCTT TGGCTGCGAA CTACCGAACT
    CCTTACGATG ACGCGTACTA TTTCTATGGG GCCAGGAATA CTTTGGATCC TAGTCTTGCT
    TATTATGGTT CAGGGATGAT GGGAGTACAG CCTGCTCCTG CTCCTTATGT TACAGCTCCT
    GTCACCCACC AGCCAGTACA GCCTATTGTG AGTACAGTTG GACAGAATGA GCTGAAAATT
    ACAAGTAATC GAGCAATAAA TTCAGGATTG TTTTTTGAAG ATAAACCAAA GCCTTCAAAA
    CAGTCATTTC AGTCTTACCA GGAAGCTTTG CAGCAGCAGA TTCAAGAAAG ACAAGAAAGA
    AGAAAGAAAG AACGTGAAGA AAAAGAAGTA TATGAAGCCA AATTAGAAGC TGAAATGAGA
    AGTTACAACC CCTGGGGGAA AGGTGGAGGT GGTGCTCCTC TGAGAGATGA CAAAGGAAAT
    CTGATAACTG ATTTGAATAG GATGCACAGA CAAAATATAG ATGCCTACCA TAACCCAGAT
    GCAAGAGCAT ATGAAGATAA AAGGGCTGTT GTATCTCTAG ACCAAAATTT AGCCACTTCA
    AATGCGGAGA ACCAAGAAGA TGCTGCAAAT AAAAACTCAG GTCATATGCA ATCACAGAGC
    TCTCCTTTTG CTCGGGGAAA TATTTTTAGT GAGCCTCCAA CTGAACTTCA AATTAAACAG
    CAGGAATTAT ACAAGAATTT TCTTCGTTTT CAGATTGAGG AAAAGAAGCA AAGAGAAGAA
    GCAGAGCGAG AGAGATTAAG AATTGCTGAA GAAAAAGAAG AAAAACGGCT TGCAGAACAG
    AGAGCTCGAA TCCAGCAAGA GTATGAAGAG GAGCAGGAAA AGAAAAGGGA GAAAGAGGAG
    GAGCAAAGAC TGAAAAACGA AGAGCTTATT CGGTTAGCTG AAGAAAGACG AAAAGAAGCA
    GAAAGAAAGA AGAAAGAAGA AGAAGAAAAA CATAATCTAC AACTTCAGCA CTACTATGAA
    AGAGAAAACA TGATTGGGGA AGATACAAAG CACTTGAGAC AGCCTTCTCC TGTGGTTCCT
    GCTCTTCAGA ATAAGATTGC AAGCAGACTC CAAAGACCTC CTTCAGTTGA CAGCATTATA
    ACTTCCTTTA CTCAGGACAG TTCCATGTCC ATGGCACAAT CTCCTCCGGT ACCTGCCAGG
    AAAAACCAGC TTCGTGCAGA AGAGGAGAAA AAAAATGTAA TTATGGAATT ATCAGAAATG
    AGAAAACAGC TTCGTAGTGA AGAGCGACGT CTGCAGGGGC GGTTACTACA CATTGATAGT
    GACGACGACA TTCATATAAG GAAAAGAGAA AGTTCCATGG ATGTATTTGA TATGGCCAGA
    CATCGATTAC AAGCTCCTGT CAGAAGACCG TCACCTAAGA GCTTAGACTC TGCTTCTTCT
    CAGAATATTC ATGACTTTAA GGAGCTGAAA GATAGAGATT CAGAAACGCG AGCTGATCTG
    AAATCTATGT ACCCAGATGA TCCAAGAGAT AATGACACCT TGGAGATAGA ACAACAAGCC
    TTGCTAAGAG AGCAGCAGAA GAGACTGAAT AAACTAAAAA TGCAGGAAGT TGACTTGGAT
    GCTGTCCCAA GCACTAAAGT ACGGGACCAC AGAATGCCCA GAGAGGACAC TGATGATTTC
    TTGAAAAACT CATTATTAGA ATCTGATAGT GCTTTTATTG GTGCGCATGG CGAGACGTAC
    CCAGCCATTG AGGATGGGGG CTTCCTGCTG CTATCTCCGC TGCCCTCCGC CAGAGAGCGC
    AGAAGGAACA AACTGAAAGG ATTGGACTTT GATAACAGTC ACCCTGATGT GCCACCAGAT
    GGTGTCTCTT TAAAATCTGT ATCCAGTATA AATATTGATC AGCTTAGAAT GAGAAATGAA
    GAACGAATGC GAAGACTGAA TGAACTTCAG AATAAACCTA TTACTACAGA TGATGAGAGT
    TCACTGGTGG ACCCTGATGA CATCATGAGG CACCGGGGTG ACGACGGATC AAACTCTGTA
    GCCACTGAGC CCTGGCTCCG CCCAGGCACC TCAGAAACAC TGAAGCGATT CATGGTGGAA
    CAGCTGAGGC AGGAGCAGCA GCAGGGTCCT GGCAAACCAG GCGCTTTCAC CTGGCAGGGA
    CTGTCTACTG CACACGGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50845
    Clone ID Related Accession (Same CDS sequence) XM_010812038.3 , XM_010812038.2
    Accession Version XM_010812038.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGTTGA CTTGGATGCT GTCCCAAGCA CTAAAGTACG GGACCACAGA
    ATGCCCAGAG AGGACACTGA TGATTTCTTG AAAAACTCAT TATTAGAATC TGATAGTGCT
    TTTATTGGTG CGCATGGCGA GACGTACCCA GCCATTGAGG ATGGGGGCTT CCTGCTGCTA
    TCTCCGCTGC CCTCCGCCAG AGAGCGCAGA AGGAACAAAC TGAAAGGATT GGACTTTGAT
    AACAGTCACC CTGATGTGCC ACCAGATGGT GTCTCTTTAA AATCTGTATC CAGTATAAAT
    ATTGATCAGC TTAGAATGAG AAATGAAGAA CGAATGCGAA GACTGAATGA ACTTCAGAAT
    AAACCTATTA CTACAGATGA TGAGAGTTCA CTGGTGGACC CTGATGACAT CATGAGGCAC
    CGGGGTGACG ACGGATCAAA CTCTGTAGCC ACTGAGCCCT GGCTCCGCCC AGGCACCTCA
    GAAACACTGA AGCGATTCAT GGTGGAACAG CTGAGGCAGG AGCAGCAGCA GGGTCCTGGC
    AAACCAGGCG CTTTCACCTG GCAGGGACTG TCTACTGCAC ACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810340.1
    CDS183..3710
    Translation

    Target ORF information:

    RefSeq Version XM_010812038.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812038.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGTTGA CTTGGATGCT GTCCCAAGCA CTAAAGTACG GGACCACAGA
    ATGCCCAGAG AGGACACTGA TGATTTCTTG AAAAACTCAT TATTAGAATC TGATAGTGCT
    TTTATTGGTG CGCATGGCGA GACGTACCCA GCCATTGAGG ATGGGGGCTT CCTGCTGCTA
    TCTCCGCTGC CCTCCGCCAG AGAGCGCAGA AGGAACAAAC TGAAAGGATT GGACTTTGAT
    AACAGTCACC CTGATGTGCC ACCAGATGGT GTCTCTTTAA AATCTGTATC CAGTATAAAT
    ATTGATCAGC TTAGAATGAG AAATGAAGAA CGAATGCGAA GACTGAATGA ACTTCAGAAT
    AAACCTATTA CTACAGATGA TGAGAGTTCA CTGGTGGACC CTGATGACAT CATGAGGCAC
    CGGGGTGACG ACGGATCAAA CTCTGTAGCC ACTGAGCCCT GGCTCCGCCC AGGCACCTCA
    GAAACACTGA AGCGATTCAT GGTGGAACAG CTGAGGCAGG AGCAGCAGCA GGGTCCTGGC
    AAACCAGGCG CTTTCACCTG GCAGGGACTG TCTACTGCAC ACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50845
    Clone ID Related Accession (Same CDS sequence) XM_010812038.3 , XM_010812038.2
    Accession Version XM_010812038.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGTTGA CTTGGATGCT GTCCCAAGCA CTAAAGTACG GGACCACAGA
    ATGCCCAGAG AGGACACTGA TGATTTCTTG AAAAACTCAT TATTAGAATC TGATAGTGCT
    TTTATTGGTG CGCATGGCGA GACGTACCCA GCCATTGAGG ATGGGGGCTT CCTGCTGCTA
    TCTCCGCTGC CCTCCGCCAG AGAGCGCAGA AGGAACAAAC TGAAAGGATT GGACTTTGAT
    AACAGTCACC CTGATGTGCC ACCAGATGGT GTCTCTTTAA AATCTGTATC CAGTATAAAT
    ATTGATCAGC TTAGAATGAG AAATGAAGAA CGAATGCGAA GACTGAATGA ACTTCAGAAT
    AAACCTATTA CTACAGATGA TGAGAGTTCA CTGGTGGACC CTGATGACAT CATGAGGCAC
    CGGGGTGACG ACGGATCAAA CTCTGTAGCC ACTGAGCCCT GGCTCCGCCC AGGCACCTCA
    GAAACACTGA AGCGATTCAT GGTGGAACAG CTGAGGCAGG AGCAGCAGCA GGGTCCTGGC
    AAACCAGGCG CTTTCACCTG GCAGGGACTG TCTACTGCAC ACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810340.1
    CDS114..3641
    Translation

    Target ORF information:

    RefSeq Version XM_010812038.2
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812038.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAACTGA TTTGAATAGG
    ATGCACAGAC AAAATATAGA TGCCTACCAT AACCCAGATG CAAGAGCATA TGAAGATAAA
    AGGGCTGTTG TATCTCTAGA CCAAAATTTA GCCACTTCAA ATGCGGAGAA CCAAGAAGAT
    GCTGCAAATA AAAACTCAGG TCATATGCAA TCACAGAGCT CTCCTTTTGC TCGGGGAAAT
    ATTTTTAGTG AGCCTCCAAC TGAACTTCAA ATTAAACAGC AGGAATTATA CAAGAATTTT
    CTTCGTTTTC AGATTGAGGA AAAGAAGCAA AGAGAAGAAG CAGAGCGAGA GAGATTAAGA
    ATTGCTGAAG AAAAAGAAGA AAAACGGCTT GCAGAACAGA GAGCTCGAAT CCAGCAAGAG
    TATGAAGAGG AGCAGGAAAA GAAAAGGGAG AAAGAGGAGG AGCAAAGACT GAAAAACGAA
    GAGCTTATTC GGTTAGCTGA AGAAAGACGA AAAGAAGCAG AAAGAAAGAA GAAAGAAGAA
    GAAGAAAAAC ATAATCTACA ACTTCAGCAC TACTATGAAA GAGAAAACAT GATTGGGGAA
    GATACAAAGC ACTTGAGACA GCCTTCTCCT GTGGTTCCTG CTCTTCAGAA TAAGATTGCA
    AGCAGACTCC AAAGACCTCC TTCAGTTGAC AGCATTATAA CTTCCTTTAC TCAGGACAGT
    TCCATGTCCA TGGCACAATC TCCTCCGGTA CCTGCCAGGA AAAACCAGCT TCGTGCAGAA
    GAGGAGAAAA AAAATGTAAT TATGGAATTA TCAGAAATGA GAAAACAGCT TCGTAGTGAA
    GAGCGACGTC TGCAGGGGCG GTTACTACAC ATTGATAGTG ACGACGACAT TCATATAAGG
    AAAAGAGAAA GTTCCATGGA TGTATTTGAT ATGGCCAGAC ATCGATTACA AGCTCCTGTC
    AGAAGACCGT CACCTAAGAG CTTAGACTCT GCTTCTTCTC AGAATATTCA TGACTTTAAG
    GAGCTGAAAG ATAGAGTTGA CTTGGATGCT GTCCCAAGCA CTAAAGTACG GGACCACAGA
    ATGCCCAGAG AGGACACTGA TGATTTCTTG AAAAACTCAT TATTAGAATC TGATAGTGCT
    TTTATTGGTG CGCATGGCGA GACGTACCCA GCCATTGAGG ATGGGGGCTT CCTGCTGCTA
    TCTCCGCTGC CCTCCGCCAG AGAGCGCAGA AGGAACAAAC TGAAAGGATT GGACTTTGAT
    AACAGTCACC CTGATGTGCC ACCAGATGGT GTCTCTTTAA AATCTGTATC CAGTATAAAT
    ATTGATCAGC TTAGAATGAG AAATGAAGAA CGAATGCGAA GACTGAATGA ACTTCAGAAT
    AAACCTATTA CTACAGATGA TGAGAGTTCA CTGGTGGACC CTGATGACAT CATGAGGCAC
    CGGGGTGACG ACGGATCAAA CTCTGTAGCC ACTGAGCCCT GGCTCCGCCC AGGCACCTCA
    GAAACACTGA AGCGATTCAT GGTGGAACAG CTGAGGCAGG AGCAGCAGCA GGGTCCTGGC
    AAACCAGGCG CTTTCACCTG GCAGGGACTG TCTACTGCAC ACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50846
    Clone ID Related Accession (Same CDS sequence) XM_005215484.4 , XM_005215484.3
    Accession Version XM_005215484.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215484.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAAGTCA TATGCAATCA
    CAGAGCTCTC CTTTTGCTCG GGGAAATATT TTTAGTGAGC CTCCAACTGA ACTTCAAATT
    AAACAGCAGG AATTATACAA GAATTTTCTT CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA
    GAAGAAGCAG AGCGAGAGAG ATTAAGAATT GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA
    GAACAGAGAG CTCGAATCCA GCAAGAGTAT GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA
    GAGGAGGAGC AAAGACTGAA AAACGAAGAG CTTATTCGGT TAGCTGAAGA AAGACGAAAA
    GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA GAAAAACATA ATCTACAACT TCAGCACTAC
    TATGAAAGAG AAAACATGAT TGGGGAAGAT ACAAAGCACT TGAGACAGCC TTCTCCTGTG
    GTTCCTGCTC TTCAGAATAA GATTGCAAGC AGACTCCAAA GACCTCCTTC AGTTGACAGC
    ATTATAACTT CCTTTACTCA GGACAGTTCC ATGTCCATGG CACAATCTCC TCCGGTACCT
    GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG GAGAAAAAAA ATGTAATTAT GGAATTATCA
    GAAATGAGAA AACAGCTTCG TAGTGAAGAG CGACGTCTGC AGGGGCGGTT ACTACACATT
    GATAGTGACG ACGACATTCA TATAAGGAAA AGAGAAAGTT CCATGGATGT ATTTGATATG
    GCCAGACATC GATTACAAGC TCCTGTCAGA AGACCGTCAC CTAAGAGCTT AGACTCTGCT
    TCTTCTCAGA ATATTCATGA CTTTAAGGAG CTGAAAGATA GAGATTCAGA AACGCGAGCT
    GATCTGAAAT CTATGTACCC AGATGATCCA AGAGATAATG ACACCTTGGA GATAGAACAA
    CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA CTGAATAAAC TAAAAATGCA GGAAGTTGAC
    TTGGATGCTG TCCCAAGCAC TAAAGTACGG GACCACAGAA TGCCCAGAGA GGACACTGAT
    GATTTCTTGA AAAACTCATT ATTAGAATCT GATAGTGCTT TTATTGGTGC GCATGGCGAG
    ACGTACCCAG CCATTGAGGA TGGGGGCTTC CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA
    GAGCGCAGAA GGAACAAACT GAAAGGATTG GACTTTGATA ACAGTCACCC TGATGTGCCA
    CCAGATGGTG TCTCTTTAAA ATCTGTATCC AGTATAAATA TTGATCAGCT TAGAATGAGA
    AATGAAGAAC GAATGCGAAG ACTGAATGAA CTTCAGAATA AACCTATTAC TACAGATGAT
    GAGAGTTCAC TGGTGGACCC TGATGACATC ATGAGGCACC GGGGTGACGA CGGATCAAAC
    TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA GGCACCTCAG AAACACTGAA GCGATTCATG
    GTGGAACAGC TGAGGCAGGA GCAGCAGCAG GGTCCTGGCA AACCAGGCGC TTTCACCTGG
    CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215541.2
    CDS183..3689
    Translation

    Target ORF information:

    RefSeq Version XM_005215484.4
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215484.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAAGTCA TATGCAATCA
    CAGAGCTCTC CTTTTGCTCG GGGAAATATT TTTAGTGAGC CTCCAACTGA ACTTCAAATT
    AAACAGCAGG AATTATACAA GAATTTTCTT CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA
    GAAGAAGCAG AGCGAGAGAG ATTAAGAATT GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA
    GAACAGAGAG CTCGAATCCA GCAAGAGTAT GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA
    GAGGAGGAGC AAAGACTGAA AAACGAAGAG CTTATTCGGT TAGCTGAAGA AAGACGAAAA
    GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA GAAAAACATA ATCTACAACT TCAGCACTAC
    TATGAAAGAG AAAACATGAT TGGGGAAGAT ACAAAGCACT TGAGACAGCC TTCTCCTGTG
    GTTCCTGCTC TTCAGAATAA GATTGCAAGC AGACTCCAAA GACCTCCTTC AGTTGACAGC
    ATTATAACTT CCTTTACTCA GGACAGTTCC ATGTCCATGG CACAATCTCC TCCGGTACCT
    GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG GAGAAAAAAA ATGTAATTAT GGAATTATCA
    GAAATGAGAA AACAGCTTCG TAGTGAAGAG CGACGTCTGC AGGGGCGGTT ACTACACATT
    GATAGTGACG ACGACATTCA TATAAGGAAA AGAGAAAGTT CCATGGATGT ATTTGATATG
    GCCAGACATC GATTACAAGC TCCTGTCAGA AGACCGTCAC CTAAGAGCTT AGACTCTGCT
    TCTTCTCAGA ATATTCATGA CTTTAAGGAG CTGAAAGATA GAGATTCAGA AACGCGAGCT
    GATCTGAAAT CTATGTACCC AGATGATCCA AGAGATAATG ACACCTTGGA GATAGAACAA
    CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA CTGAATAAAC TAAAAATGCA GGAAGTTGAC
    TTGGATGCTG TCCCAAGCAC TAAAGTACGG GACCACAGAA TGCCCAGAGA GGACACTGAT
    GATTTCTTGA AAAACTCATT ATTAGAATCT GATAGTGCTT TTATTGGTGC GCATGGCGAG
    ACGTACCCAG CCATTGAGGA TGGGGGCTTC CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA
    GAGCGCAGAA GGAACAAACT GAAAGGATTG GACTTTGATA ACAGTCACCC TGATGTGCCA
    CCAGATGGTG TCTCTTTAAA ATCTGTATCC AGTATAAATA TTGATCAGCT TAGAATGAGA
    AATGAAGAAC GAATGCGAAG ACTGAATGAA CTTCAGAATA AACCTATTAC TACAGATGAT
    GAGAGTTCAC TGGTGGACCC TGATGACATC ATGAGGCACC GGGGTGACGA CGGATCAAAC
    TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA GGCACCTCAG AAACACTGAA GCGATTCATG
    GTGGAACAGC TGAGGCAGGA GCAGCAGCAG GGTCCTGGCA AACCAGGCGC TTTCACCTGG
    CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50846
    Clone ID Related Accession (Same CDS sequence) XM_005215484.4 , XM_005215484.3
    Accession Version XM_005215484.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAAGTCA TATGCAATCA
    CAGAGCTCTC CTTTTGCTCG GGGAAATATT TTTAGTGAGC CTCCAACTGA ACTTCAAATT
    AAACAGCAGG AATTATACAA GAATTTTCTT CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA
    GAAGAAGCAG AGCGAGAGAG ATTAAGAATT GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA
    GAACAGAGAG CTCGAATCCA GCAAGAGTAT GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA
    GAGGAGGAGC AAAGACTGAA AAACGAAGAG CTTATTCGGT TAGCTGAAGA AAGACGAAAA
    GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA GAAAAACATA ATCTACAACT TCAGCACTAC
    TATGAAAGAG AAAACATGAT TGGGGAAGAT ACAAAGCACT TGAGACAGCC TTCTCCTGTG
    GTTCCTGCTC TTCAGAATAA GATTGCAAGC AGACTCCAAA GACCTCCTTC AGTTGACAGC
    ATTATAACTT CCTTTACTCA GGACAGTTCC ATGTCCATGG CACAATCTCC TCCGGTACCT
    GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG GAGAAAAAAA ATGTAATTAT GGAATTATCA
    GAAATGAGAA AACAGCTTCG TAGTGAAGAG CGACGTCTGC AGGGGCGGTT ACTACACATT
    GATAGTGACG ACGACATTCA TATAAGGAAA AGAGAAAGTT CCATGGATGT ATTTGATATG
    GCCAGACATC GATTACAAGC TCCTGTCAGA AGACCGTCAC CTAAGAGCTT AGACTCTGCT
    TCTTCTCAGA ATATTCATGA CTTTAAGGAG CTGAAAGATA GAGATTCAGA AACGCGAGCT
    GATCTGAAAT CTATGTACCC AGATGATCCA AGAGATAATG ACACCTTGGA GATAGAACAA
    CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA CTGAATAAAC TAAAAATGCA GGAAGTTGAC
    TTGGATGCTG TCCCAAGCAC TAAAGTACGG GACCACAGAA TGCCCAGAGA GGACACTGAT
    GATTTCTTGA AAAACTCATT ATTAGAATCT GATAGTGCTT TTATTGGTGC GCATGGCGAG
    ACGTACCCAG CCATTGAGGA TGGGGGCTTC CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA
    GAGCGCAGAA GGAACAAACT GAAAGGATTG GACTTTGATA ACAGTCACCC TGATGTGCCA
    CCAGATGGTG TCTCTTTAAA ATCTGTATCC AGTATAAATA TTGATCAGCT TAGAATGAGA
    AATGAAGAAC GAATGCGAAG ACTGAATGAA CTTCAGAATA AACCTATTAC TACAGATGAT
    GAGAGTTCAC TGGTGGACCC TGATGACATC ATGAGGCACC GGGGTGACGA CGGATCAAAC
    TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA GGCACCTCAG AAACACTGAA GCGATTCATG
    GTGGAACAGC TGAGGCAGGA GCAGCAGCAG GGTCCTGGCA AACCAGGCGC TTTCACCTGG
    CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215541.2
    CDS114..3620
    Translation

    Target ORF information:

    RefSeq Version XM_005215484.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215484.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG GATGTACGGG
    AACAGACGAG GCAATGAGCC TCCTATGGAG TGTGATGGTG ATGTCACAGA ACAGTCAAAC
    ATACGAATTT CATCTGCTGG AAATCAAAGT GCTCGAGACA ATGAACCATC CAAACCTACT
    TATCGAGAGA CATGCAGTCC TTTTGCGGGA ATGCTCTTTG GGGGTGAAGA TCGAGAACTT
    ATTCAGAGAA GGAAAGAGAA ATATAGACAA GAACTATTGG AACAAATGGC TGAACAACAG
    AGGAACAAGA GACGAGAAAA AGATTTGGAT CTCAGGGTTG CAGCTTCTGG AGCACAAGAC
    CCTGAGAAAT CGCCTGATAG ACTAAAGCAG TTTAGTGTGG CACCAAGACA CTTTGAAGAG
    ATGATACCAC CTGAGAGACC CAGAATAGCT TTCCAGACAC CTCTCCCTCC TCTGTCCGCC
    CCATCTGTCC CACCCATCCC ATCAGTTTAC TCCATCCCAT CTCAGAGTGA AGATGTGCAC
    AGTGGACTCA GCAGCACTCT TGGTGACATG GTGCCTCCCA GAGTTGCACC TCTGCCTCCA
    CCTCCCATGC TGCCCCCTTT GGCTGCGAAC TACCGAACTC CTTACGATGA CGCGTACTAT
    TTCTATGGGG CCAGGAATAC TTTGGATCCT AGTCTTGCTT ATTATGGTTC AGGGATGATG
    GGAGTACAGC CTGCTCCTGC TCCTTATGTT ACAGCTCCTG TCACCCACCA GCCAGTACAG
    CCTATTGTGA GTACAGTTGG ACAGAATGAG CTGAAAATTA CAAGTAATCG AGCAATAAAT
    TCAGGATTGT TTTTTGAAGA TAAACCAAAG CCTTCAAAAC AGTCATTTCA GTCTTACCAG
    GAAGCTTTGC AGCAGCAGAT TCAAGAAAGA CAAGAAAGAA GAAAGAAAGA ACGTGAAGAA
    AAAGAAGTAT ATGAAGCCAA ATTAGAAGCT GAAATGAGAA GTTACAACCC CTGGGGGAAA
    GGTGGAGGTG GTGCTCCTCT GAGAGATGAC AAAGGAAATC TGATAAGTCA TATGCAATCA
    CAGAGCTCTC CTTTTGCTCG GGGAAATATT TTTAGTGAGC CTCCAACTGA ACTTCAAATT
    AAACAGCAGG AATTATACAA GAATTTTCTT CGTTTTCAGA TTGAGGAAAA GAAGCAAAGA
    GAAGAAGCAG AGCGAGAGAG ATTAAGAATT GCTGAAGAAA AAGAAGAAAA ACGGCTTGCA
    GAACAGAGAG CTCGAATCCA GCAAGAGTAT GAAGAGGAGC AGGAAAAGAA AAGGGAGAAA
    GAGGAGGAGC AAAGACTGAA AAACGAAGAG CTTATTCGGT TAGCTGAAGA AAGACGAAAA
    GAAGCAGAAA GAAAGAAGAA AGAAGAAGAA GAAAAACATA ATCTACAACT TCAGCACTAC
    TATGAAAGAG AAAACATGAT TGGGGAAGAT ACAAAGCACT TGAGACAGCC TTCTCCTGTG
    GTTCCTGCTC TTCAGAATAA GATTGCAAGC AGACTCCAAA GACCTCCTTC AGTTGACAGC
    ATTATAACTT CCTTTACTCA GGACAGTTCC ATGTCCATGG CACAATCTCC TCCGGTACCT
    GCCAGGAAAA ACCAGCTTCG TGCAGAAGAG GAGAAAAAAA ATGTAATTAT GGAATTATCA
    GAAATGAGAA AACAGCTTCG TAGTGAAGAG CGACGTCTGC AGGGGCGGTT ACTACACATT
    GATAGTGACG ACGACATTCA TATAAGGAAA AGAGAAAGTT CCATGGATGT ATTTGATATG
    GCCAGACATC GATTACAAGC TCCTGTCAGA AGACCGTCAC CTAAGAGCTT AGACTCTGCT
    TCTTCTCAGA ATATTCATGA CTTTAAGGAG CTGAAAGATA GAGATTCAGA AACGCGAGCT
    GATCTGAAAT CTATGTACCC AGATGATCCA AGAGATAATG ACACCTTGGA GATAGAACAA
    CAAGCCTTGC TAAGAGAGCA GCAGAAGAGA CTGAATAAAC TAAAAATGCA GGAAGTTGAC
    TTGGATGCTG TCCCAAGCAC TAAAGTACGG GACCACAGAA TGCCCAGAGA GGACACTGAT
    GATTTCTTGA AAAACTCATT ATTAGAATCT GATAGTGCTT TTATTGGTGC GCATGGCGAG
    ACGTACCCAG CCATTGAGGA TGGGGGCTTC CTGCTGCTAT CTCCGCTGCC CTCCGCCAGA
    GAGCGCAGAA GGAACAAACT GAAAGGATTG GACTTTGATA ACAGTCACCC TGATGTGCCA
    CCAGATGGTG TCTCTTTAAA ATCTGTATCC AGTATAAATA TTGATCAGCT TAGAATGAGA
    AATGAAGAAC GAATGCGAAG ACTGAATGAA CTTCAGAATA AACCTATTAC TACAGATGAT
    GAGAGTTCAC TGGTGGACCC TGATGACATC ATGAGGCACC GGGGTGACGA CGGATCAAAC
    TCTGTAGCCA CTGAGCCCTG GCTCCGCCCA GGCACCTCAG AAACACTGAA GCGATTCATG
    GTGGAACAGC TGAGGCAGGA GCAGCAGCAG GGTCCTGGCA AACCAGGCGC TTTCACCTGG
    CAGGGACTGT CTACTGCACA CGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50847
    Clone ID Related Accession (Same CDS sequence) XM_005215487.3 , XM_005215487.4
    Accession Version XM_005215487.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104396.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG AGTTGCACCT
    CTGCCTCCAC CTCCCATGCT GCCCCCTTTG GCTGCGAACT ACCGAACTCC TTACGATGAC
    GCGTACTATT TCTATGGGGC CAGGAATACT TTGGATCCTA GTCTTGCTTA TTATGGTTCA
    GGGATGATGG GAGTACAGCC TGCTCCTGCT CCTTATGTTA CAGCTCCTGT CACCCACCAG
    CCAGTACAGC CTATTGTGAG TACAGTTGGA CAGAATGAGC TGAAAATTAC AAGTAATCGA
    GCAATAAATT CAGGATTGTT TTTTGAAGAT AAACCAAAGC CTTCAAAACA GTCATTTCAG
    TCTTACCAGG AAGCTTTGCA GCAGCAGATT CAAGAAAGAC AAGAAAGAAG AAAGAAAGAA
    CGTGAAGAAA AAGAAGTATA TGAAGCCAAA TTAGAAGCTG AAATGAGAAG TTACAACCCC
    TGGGGGAAAG GTGGAGGTGG TGCTCCTCTG AGAGATGACA AAGGAAATCT GATAACTGAT
    TTGAATAGGA TGCACAGACA AAATATAGAT GCCTACCATA ACCCAGATGC AAGAGCATAT
    GAAGATAAAA GGGCTGTTGT ATCTCTAGAC CAAAATTTAG CCACTTCAAA TGCGGAGAAC
    CAAGAAGATG CTGCAAATAA AAACTCAGGT CATATGCAAT CACAGAGCTC TCCTTTTGCT
    CGGGGAAATA TTTTTAGTGA GCCTCCAACT GAACTTCAAA TTAAACAGCA GGAATTATAC
    AAGAATTTTC TTCGTTTTCA GATTGAGGAA AAGAAGCAAA GAGAAGAAGC AGAGCGAGAG
    AGATTAAGAA TTGCTGAAGA AAAAGAAGAA AAACGGCTTG CAGAACAGAG AGCTCGAATC
    CAGCAAGAGT ATGAAGAGGA GCAGGAAAAG AAAAGGGAGA AAGAGGAGGA GCAAAGACTG
    AAAAACGAAG AGCTTATTCG GTTAGCTGAA GAAAGACGAA AAGAAGCAGA AAGAAAGAAG
    AAAGAAGAAG AAGAAAAACA TAATCTACAA CTTCAGCACT ACTATGAAAG AGAAAACATG
    ATTGGGGAAG ATACAAAGCA CTTGAGACAG CCTTCTCCTG TGGTTCCTGC TCTTCAGAAT
    AAGATTGCAA GCAGACTCCA AAGACCTCCT TCAGTTGACA GCATTATAAC TTCCTTTACT
    CAGGACAGTT CCATGTCCAT GGCACAATCT CCTCCGGTAC CTGCCAGGAA AAACCAGCTT
    CGTGCAGAAG AGGAGAAAAA AAATGTAATT ATGGAATTAT CAGAAATGAG AAAACAGCTT
    CGTAGTGAAG AGCGACGTCT GCAGGGGCGG TTACTACACA TTGATAGTGA CGACGACATT
    CATATAAGGA AAAGAGAAAG TTCCATGGAT GTATTTGATA TGGCCAGACA TCGATTACAA
    GCTCCTGTCA GAAGACCGTC ACCTAAGAGC TTAGACTCTG CTTCTTCTCA GAATATTCAT
    GACTTTAAGG AGCTGAAAGA TAGAGATTCA GAAACGCGAG CTGATCTGAA ATCTATGTAC
    CCAGATGATC CAAGAGATAA TGACACCTTG GAGATAGAAC AACAAGCCTT GCTAAGAGAG
    CAGCAGAAGA GACTGAATAA ACTAAAAATG CAGGAAGTTG ACTTGGATGC TGTCCCAAGC
    ACTAAAGTAC GGGACCACAG AATGCCCAGA GAGGACACTG ATGATTTCTT GAAAAACTCA
    TTATTAGAAT CTGATAGTGC TTTTATTGGT GCGCATGGCG AGACGTACCC AGCCATTGAG
    GATGGGGGCT TCCTGCTGCT ATCTCCGCTG CCCTCCGCCA GAGAGCGCAG AAGGAACAAA
    CTGAAAGGAT TGGACTTTGA TAACAGTCAC CCTGATGTGC CACCAGATGG TGTCTCTTTA
    AAATCTGTAT CCAGTATAAA TATTGATCAG CTTAGAATGA GAAATGAAGA ACGAATGCGA
    AGACTGAATG AACTTCAGAA TAAACCTATT ACTACAGATG ATGAGAGTTC ACTGGTGGAC
    CCTGATGACA TCATGAGGCA CCGGGGTGAC GACGGATCAA ACTCTGTAGC CACTGAGCCC
    TGGCTCCGCC CAGGCACCTC AGAAACACTG AAGCGATTCA TGGTGGAACA GCTGAGGCAG
    GAGCAGCAGC AGGGTCCTGG CAAACCAGGC GCTTTCACCT GGCAGGGACT GTCTACTGCA
    CACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215544.2
    CDS114..3242
    Translation

    Target ORF information:

    RefSeq Version XM_005215487.3
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215487.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG AGTTGCACCT
    CTGCCTCCAC CTCCCATGCT GCCCCCTTTG GCTGCGAACT ACCGAACTCC TTACGATGAC
    GCGTACTATT TCTATGGGGC CAGGAATACT TTGGATCCTA GTCTTGCTTA TTATGGTTCA
    GGGATGATGG GAGTACAGCC TGCTCCTGCT CCTTATGTTA CAGCTCCTGT CACCCACCAG
    CCAGTACAGC CTATTGTGAG TACAGTTGGA CAGAATGAGC TGAAAATTAC AAGTAATCGA
    GCAATAAATT CAGGATTGTT TTTTGAAGAT AAACCAAAGC CTTCAAAACA GTCATTTCAG
    TCTTACCAGG AAGCTTTGCA GCAGCAGATT CAAGAAAGAC AAGAAAGAAG AAAGAAAGAA
    CGTGAAGAAA AAGAAGTATA TGAAGCCAAA TTAGAAGCTG AAATGAGAAG TTACAACCCC
    TGGGGGAAAG GTGGAGGTGG TGCTCCTCTG AGAGATGACA AAGGAAATCT GATAACTGAT
    TTGAATAGGA TGCACAGACA AAATATAGAT GCCTACCATA ACCCAGATGC AAGAGCATAT
    GAAGATAAAA GGGCTGTTGT ATCTCTAGAC CAAAATTTAG CCACTTCAAA TGCGGAGAAC
    CAAGAAGATG CTGCAAATAA AAACTCAGGT CATATGCAAT CACAGAGCTC TCCTTTTGCT
    CGGGGAAATA TTTTTAGTGA GCCTCCAACT GAACTTCAAA TTAAACAGCA GGAATTATAC
    AAGAATTTTC TTCGTTTTCA GATTGAGGAA AAGAAGCAAA GAGAAGAAGC AGAGCGAGAG
    AGATTAAGAA TTGCTGAAGA AAAAGAAGAA AAACGGCTTG CAGAACAGAG AGCTCGAATC
    CAGCAAGAGT ATGAAGAGGA GCAGGAAAAG AAAAGGGAGA AAGAGGAGGA GCAAAGACTG
    AAAAACGAAG AGCTTATTCG GTTAGCTGAA GAAAGACGAA AAGAAGCAGA AAGAAAGAAG
    AAAGAAGAAG AAGAAAAACA TAATCTACAA CTTCAGCACT ACTATGAAAG AGAAAACATG
    ATTGGGGAAG ATACAAAGCA CTTGAGACAG CCTTCTCCTG TGGTTCCTGC TCTTCAGAAT
    AAGATTGCAA GCAGACTCCA AAGACCTCCT TCAGTTGACA GCATTATAAC TTCCTTTACT
    CAGGACAGTT CCATGTCCAT GGCACAATCT CCTCCGGTAC CTGCCAGGAA AAACCAGCTT
    CGTGCAGAAG AGGAGAAAAA AAATGTAATT ATGGAATTAT CAGAAATGAG AAAACAGCTT
    CGTAGTGAAG AGCGACGTCT GCAGGGGCGG TTACTACACA TTGATAGTGA CGACGACATT
    CATATAAGGA AAAGAGAAAG TTCCATGGAT GTATTTGATA TGGCCAGACA TCGATTACAA
    GCTCCTGTCA GAAGACCGTC ACCTAAGAGC TTAGACTCTG CTTCTTCTCA GAATATTCAT
    GACTTTAAGG AGCTGAAAGA TAGAGATTCA GAAACGCGAG CTGATCTGAA ATCTATGTAC
    CCAGATGATC CAAGAGATAA TGACACCTTG GAGATAGAAC AACAAGCCTT GCTAAGAGAG
    CAGCAGAAGA GACTGAATAA ACTAAAAATG CAGGAAGTTG ACTTGGATGC TGTCCCAAGC
    ACTAAAGTAC GGGACCACAG AATGCCCAGA GAGGACACTG ATGATTTCTT GAAAAACTCA
    TTATTAGAAT CTGATAGTGC TTTTATTGGT GCGCATGGCG AGACGTACCC AGCCATTGAG
    GATGGGGGCT TCCTGCTGCT ATCTCCGCTG CCCTCCGCCA GAGAGCGCAG AAGGAACAAA
    CTGAAAGGAT TGGACTTTGA TAACAGTCAC CCTGATGTGC CACCAGATGG TGTCTCTTTA
    AAATCTGTAT CCAGTATAAA TATTGATCAG CTTAGAATGA GAAATGAAGA ACGAATGCGA
    AGACTGAATG AACTTCAGAA TAAACCTATT ACTACAGATG ATGAGAGTTC ACTGGTGGAC
    CCTGATGACA TCATGAGGCA CCGGGGTGAC GACGGATCAA ACTCTGTAGC CACTGAGCCC
    TGGCTCCGCC CAGGCACCTC AGAAACACTG AAGCGATTCA TGGTGGAACA GCTGAGGCAG
    GAGCAGCAGC AGGGTCCTGG CAAACCAGGC GCTTTCACCT GGCAGGGACT GTCTACTGCA
    CACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50847
    Clone ID Related Accession (Same CDS sequence) XM_005215487.3 , XM_005215487.4
    Accession Version XM_005215487.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product centrosome and spindle pole-associated protein 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215487.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG AGTTGCACCT
    CTGCCTCCAC CTCCCATGCT GCCCCCTTTG GCTGCGAACT ACCGAACTCC TTACGATGAC
    GCGTACTATT TCTATGGGGC CAGGAATACT TTGGATCCTA GTCTTGCTTA TTATGGTTCA
    GGGATGATGG GAGTACAGCC TGCTCCTGCT CCTTATGTTA CAGCTCCTGT CACCCACCAG
    CCAGTACAGC CTATTGTGAG TACAGTTGGA CAGAATGAGC TGAAAATTAC AAGTAATCGA
    GCAATAAATT CAGGATTGTT TTTTGAAGAT AAACCAAAGC CTTCAAAACA GTCATTTCAG
    TCTTACCAGG AAGCTTTGCA GCAGCAGATT CAAGAAAGAC AAGAAAGAAG AAAGAAAGAA
    CGTGAAGAAA AAGAAGTATA TGAAGCCAAA TTAGAAGCTG AAATGAGAAG TTACAACCCC
    TGGGGGAAAG GTGGAGGTGG TGCTCCTCTG AGAGATGACA AAGGAAATCT GATAACTGAT
    TTGAATAGGA TGCACAGACA AAATATAGAT GCCTACCATA ACCCAGATGC AAGAGCATAT
    GAAGATAAAA GGGCTGTTGT ATCTCTAGAC CAAAATTTAG CCACTTCAAA TGCGGAGAAC
    CAAGAAGATG CTGCAAATAA AAACTCAGGT CATATGCAAT CACAGAGCTC TCCTTTTGCT
    CGGGGAAATA TTTTTAGTGA GCCTCCAACT GAACTTCAAA TTAAACAGCA GGAATTATAC
    AAGAATTTTC TTCGTTTTCA GATTGAGGAA AAGAAGCAAA GAGAAGAAGC AGAGCGAGAG
    AGATTAAGAA TTGCTGAAGA AAAAGAAGAA AAACGGCTTG CAGAACAGAG AGCTCGAATC
    CAGCAAGAGT ATGAAGAGGA GCAGGAAAAG AAAAGGGAGA AAGAGGAGGA GCAAAGACTG
    AAAAACGAAG AGCTTATTCG GTTAGCTGAA GAAAGACGAA AAGAAGCAGA AAGAAAGAAG
    AAAGAAGAAG AAGAAAAACA TAATCTACAA CTTCAGCACT ACTATGAAAG AGAAAACATG
    ATTGGGGAAG ATACAAAGCA CTTGAGACAG CCTTCTCCTG TGGTTCCTGC TCTTCAGAAT
    AAGATTGCAA GCAGACTCCA AAGACCTCCT TCAGTTGACA GCATTATAAC TTCCTTTACT
    CAGGACAGTT CCATGTCCAT GGCACAATCT CCTCCGGTAC CTGCCAGGAA AAACCAGCTT
    CGTGCAGAAG AGGAGAAAAA AAATGTAATT ATGGAATTAT CAGAAATGAG AAAACAGCTT
    CGTAGTGAAG AGCGACGTCT GCAGGGGCGG TTACTACACA TTGATAGTGA CGACGACATT
    CATATAAGGA AAAGAGAAAG TTCCATGGAT GTATTTGATA TGGCCAGACA TCGATTACAA
    GCTCCTGTCA GAAGACCGTC ACCTAAGAGC TTAGACTCTG CTTCTTCTCA GAATATTCAT
    GACTTTAAGG AGCTGAAAGA TAGAGATTCA GAAACGCGAG CTGATCTGAA ATCTATGTAC
    CCAGATGATC CAAGAGATAA TGACACCTTG GAGATAGAAC AACAAGCCTT GCTAAGAGAG
    CAGCAGAAGA GACTGAATAA ACTAAAAATG CAGGAAGTTG ACTTGGATGC TGTCCCAAGC
    ACTAAAGTAC GGGACCACAG AATGCCCAGA GAGGACACTG ATGATTTCTT GAAAAACTCA
    TTATTAGAAT CTGATAGTGC TTTTATTGGT GCGCATGGCG AGACGTACCC AGCCATTGAG
    GATGGGGGCT TCCTGCTGCT ATCTCCGCTG CCCTCCGCCA GAGAGCGCAG AAGGAACAAA
    CTGAAAGGAT TGGACTTTGA TAACAGTCAC CCTGATGTGC CACCAGATGG TGTCTCTTTA
    AAATCTGTAT CCAGTATAAA TATTGATCAG CTTAGAATGA GAAATGAAGA ACGAATGCGA
    AGACTGAATG AACTTCAGAA TAAACCTATT ACTACAGATG ATGAGAGTTC ACTGGTGGAC
    CCTGATGACA TCATGAGGCA CCGGGGTGAC GACGGATCAA ACTCTGTAGC CACTGAGCCC
    TGGCTCCGCC CAGGCACCTC AGAAACACTG AAGCGATTCA TGGTGGAACA GCTGAGGCAG
    GAGCAGCAGC AGGGTCCTGG CAAACCAGGC GCTTTCACCT GGCAGGGACT GTCTACTGCA
    CACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215544.2
    CDS181..3309
    Translation

    Target ORF information:

    RefSeq Version XM_005215487.4
    Organism Bos taurus(cattle)
    Definition Bos taurus centrosome and spindle pole associated protein 1 (CSPP1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215487.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTGATA ATTTGGATGA ATTTATTGAA GAGCGAAAAG TCAGATTGGC CAGAGACAAA 
    GCAGAATTGG AAAGTGATCC ACCGTACATG GAAATGAAGG GAAAAGCATC AGAGAAGCTT
    TCTGAAAACA GTAAAATATT AATTTCCATG GCTAAGGAAA ACATACCACC TAACAGTCAA
    CAGACAAGGC GTTCTTTAGG AATTGATTAT GGATTAAGTT TACCACTTGG GGAAGAGTAT
    GAACAGAAGA AACATAAACT GAAAGAAGAA TTGCGACAAG ATTACAGACG CTATCTTACT
    CAGGGTATTA CACAAGCAAA AAGAAAGAAA AATTTTCTAT CCACGAGTGA AACAGATCCA
    TCTACTCTGG GAGTTTCCCT TCCTATTGGT GAGAGGTTAT CTGCTAAGGA AAGGTTGAAA
    CTTGAACGTA ACAAAGAATA CAATCAATTT CTCAGGGATA AGGAAGAATC CACTGAAAAG
    TTCAGACAGG TAGAAAAGAG TACTGAGCAG AAGAATCAAA GAAGTAAAAA ACCTATCAGT
    CAGGTTAAAC CTGATTTACC TTCACAAATA CAATCATCTT TCGAAAATTC AGAGGGTCCT
    AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC TTCTAAACCA AAGACGACTA
    GAAGAGGACA GGTACCGAAA ATTAGATGAT GAAATTGAAT TAAGGAACAA TAGAAGAGTC
    ATTAAAAAGA CTGATGAAGA AGTGGGCGTT TCCAGTAAAA AACATCAAAG GTTTGACAGC
    ACGACTGACA TTCCAGATAG AAGATTTCAC AGGTTTAATG AGGATCTTGC TTTTGATAAA
    CAGTCTTACA GACCAGGCTA TGATCCAGAA GTAAGTGAAG ACATGGATGA GAGGTTTAGA
    TATGAAAGTG ATTTTGATAG AAGACTTTTG AGAGTTTATA CAAAAAACAG AGTTGCACCT
    CTGCCTCCAC CTCCCATGCT GCCCCCTTTG GCTGCGAACT ACCGAACTCC TTACGATGAC
    GCGTACTATT TCTATGGGGC CAGGAATACT TTGGATCCTA GTCTTGCTTA TTATGGTTCA
    GGGATGATGG GAGTACAGCC TGCTCCTGCT CCTTATGTTA CAGCTCCTGT CACCCACCAG
    CCAGTACAGC CTATTGTGAG TACAGTTGGA CAGAATGAGC TGAAAATTAC AAGTAATCGA
    GCAATAAATT CAGGATTGTT TTTTGAAGAT AAACCAAAGC CTTCAAAACA GTCATTTCAG
    TCTTACCAGG AAGCTTTGCA GCAGCAGATT CAAGAAAGAC AAGAAAGAAG AAAGAAAGAA
    CGTGAAGAAA AAGAAGTATA TGAAGCCAAA TTAGAAGCTG AAATGAGAAG TTACAACCCC
    TGGGGGAAAG GTGGAGGTGG TGCTCCTCTG AGAGATGACA AAGGAAATCT GATAACTGAT
    TTGAATAGGA TGCACAGACA AAATATAGAT GCCTACCATA ACCCAGATGC AAGAGCATAT
    GAAGATAAAA GGGCTGTTGT ATCTCTAGAC CAAAATTTAG CCACTTCAAA TGCGGAGAAC
    CAAGAAGATG CTGCAAATAA AAACTCAGGT CATATGCAAT CACAGAGCTC TCCTTTTGCT
    CGGGGAAATA TTTTTAGTGA GCCTCCAACT GAACTTCAAA TTAAACAGCA GGAATTATAC
    AAGAATTTTC TTCGTTTTCA GATTGAGGAA AAGAAGCAAA GAGAAGAAGC AGAGCGAGAG
    AGATTAAGAA TTGCTGAAGA AAAAGAAGAA AAACGGCTTG CAGAACAGAG AGCTCGAATC
    CAGCAAGAGT ATGAAGAGGA GCAGGAAAAG AAAAGGGAGA AAGAGGAGGA GCAAAGACTG
    AAAAACGAAG AGCTTATTCG GTTAGCTGAA GAAAGACGAA AAGAAGCAGA AAGAAAGAAG
    AAAGAAGAAG AAGAAAAACA TAATCTACAA CTTCAGCACT ACTATGAAAG AGAAAACATG
    ATTGGGGAAG ATACAAAGCA CTTGAGACAG CCTTCTCCTG TGGTTCCTGC TCTTCAGAAT
    AAGATTGCAA GCAGACTCCA AAGACCTCCT TCAGTTGACA GCATTATAAC TTCCTTTACT
    CAGGACAGTT CCATGTCCAT GGCACAATCT CCTCCGGTAC CTGCCAGGAA AAACCAGCTT
    CGTGCAGAAG AGGAGAAAAA AAATGTAATT ATGGAATTAT CAGAAATGAG AAAACAGCTT
    CGTAGTGAAG AGCGACGTCT GCAGGGGCGG TTACTACACA TTGATAGTGA CGACGACATT
    CATATAAGGA AAAGAGAAAG TTCCATGGAT GTATTTGATA TGGCCAGACA TCGATTACAA
    GCTCCTGTCA GAAGACCGTC ACCTAAGAGC TTAGACTCTG CTTCTTCTCA GAATATTCAT
    GACTTTAAGG AGCTGAAAGA TAGAGATTCA GAAACGCGAG CTGATCTGAA ATCTATGTAC
    CCAGATGATC CAAGAGATAA TGACACCTTG GAGATAGAAC AACAAGCCTT GCTAAGAGAG
    CAGCAGAAGA GACTGAATAA ACTAAAAATG CAGGAAGTTG ACTTGGATGC TGTCCCAAGC
    ACTAAAGTAC GGGACCACAG AATGCCCAGA GAGGACACTG ATGATTTCTT GAAAAACTCA
    TTATTAGAAT CTGATAGTGC TTTTATTGGT GCGCATGGCG AGACGTACCC AGCCATTGAG
    GATGGGGGCT TCCTGCTGCT ATCTCCGCTG CCCTCCGCCA GAGAGCGCAG AAGGAACAAA
    CTGAAAGGAT TGGACTTTGA TAACAGTCAC CCTGATGTGC CACCAGATGG TGTCTCTTTA
    AAATCTGTAT CCAGTATAAA TATTGATCAG CTTAGAATGA GAAATGAAGA ACGAATGCGA
    AGACTGAATG AACTTCAGAA TAAACCTATT ACTACAGATG ATGAGAGTTC ACTGGTGGAC
    CCTGATGACA TCATGAGGCA CCGGGGTGAC GACGGATCAA ACTCTGTAGC CACTGAGCCC
    TGGCTCCGCC CAGGCACCTC AGAAACACTG AAGCGATTCA TGGTGGAACA GCTGAGGCAG
    GAGCAGCAGC AGGGTCCTGG CAAACCAGGC GCTTTCACCT GGCAGGGACT GTCTACTGCA
    CACGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.