VWA8 cDNA ORF clone, Bos taurus(cattle)

The following VWA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VWA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221224 XM_025000014.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221225 XM_025000015.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221226 XM_025000016.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221226 XM_025000017.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221227 XM_025000018.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa221228 XM_025000019.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa221229 XM_025000020.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa24744 XM_015465667.1
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa24744 XM_002691823.4
Latest version!
Bos taurus von Willebrand factor A domain containing 8 (VWA8), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221224
    Clone ID Related Accession (Same CDS sequence) XM_025000014.1
    Accession Version XM_025000014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5700bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACG TCGCTGGACA CTCTGGAGAT
    GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA
    GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA
    GAGGGGACAG AACATGCCAT CAAGGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC
    ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCCGCCAA GTTTGCTCAG
    ATCCTGACAA GCAGCCCTCA AGTAAACGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT
    CAAGCAGCCC GGCTTCAGAG AACTCTACCA GCAGGCCGGT CTTTTGTTGC CATGGACACC
    AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855782.1
    CDS140..5839
    Translation

    Target ORF information:

    RefSeq Version XM_025000014.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACG TCGCTGGACA CTCTGGAGAT
    GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA
    GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA
    GAGGGGACAG AACATGCCAT CAAGGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC
    ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCCGCCAA GTTTGCTCAG
    ATCCTGACAA GCAGCCCTCA AGTAAACGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT
    CAAGCAGCCC GGCTTCAGAG AACTCTACCA GCAGGCCGGT CTTTTGTTGC CATGGACACC
    AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221225
    Clone ID Related Accession (Same CDS sequence) XM_025000015.1
    Accession Version XM_025000015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGGGGATCT TCCCAACCGA GGGATGGAAC
    CTGCGTCTCC TGCATCGGCA GGCGGATTCT TTACCGCTGA GCCACCCAGG AAGCCGCACG
    TTGGAGTGGG GTGGGGAGGG GCAGAAACAG GCAGATAGAA GCACGGCAGG CGTCTCCCCA
    GGTTTCTCAG GGCCACCATC CCCACCCCAG CACCCCTCAC CCACCCATCC ATGCCTGCTG
    CATCACCAAC CAGGTCGCCT ATCACGGTTC TCAGCGTTGC CTTGCCTTGG CTCACAGGGA
    GGGTCACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855783.1
    CDS70..5604
    Translation

    Target ORF information:

    RefSeq Version XM_025000015.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGGGGATCT TCCCAACCGA GGGATGGAAC
    CTGCGTCTCC TGCATCGGCA GGCGGATTCT TTACCGCTGA GCCACCCAGG AAGCCGCACG
    TTGGAGTGGG GTGGGGAGGG GCAGAAACAG GCAGATAGAA GCACGGCAGG CGTCTCCCCA
    GGTTTCTCAG GGCCACCATC CCCACCCCAG CACCCCTCAC CCACCCATCC ATGCCTGCTG
    CATCACCAAC CAGGTCGCCT ATCACGGTTC TCAGCGTTGC CTTGCCTTGG CTCACAGGGA
    GGGTCACACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221226
    Clone ID Related Accession (Same CDS sequence) XM_025000016.1 , XM_025000017.1
    Accession Version XM_025000016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCAGAAGG ATCTTTTGGG GCAAGACGTC TTTCTAATAG GACCTCCTGG GCCTCTTCGA 
    CGCTCTATTG CTATGCAGTA CTTGGAGCTG ACCAAAAAGG AGGCTGAATA CATTGCCTTG
    TCAAGAGACA CCACCGAAAC CGACCTCAAA CAGCGGCGAG AGATCCGGGC GGGCACGGCT
    TTTTACATCG ATCAGTGTGC AGTTCGTGCA GCCACAGAAG GTAGAACACT CATCCTGGAA
    GGCTTGGAAA AGGCGGAGAG GAACGTCCTG CCGGTTTTGA ACAACTTGCT GGAGAACAGG
    GAGATGCAGC TTGAGGATGG ACGCTTCCTG ATGTCTGCCG AGCGTTACGA CAAACTTCTC
    CAAGATCATA CCAAAACAGA ATTGGATGCT TGGAAGATTG TCCGAGTTAA TGAAAATTTC
    CGAGTCATTG CCTTGGGCTT GCCAGTGCCC AGGTACTCTG GGAATCCATT AGATCCCCCA
    CTCCGGTCTC GATTTCAAGC CAGAGATATT TATTATCTTC CCTTCAAGGA CCAACTTAAA
    CTGTTATATT CAGTTGGAGC CAATGTTCCT ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT
    GCCACCACTC TCTGTTCCCA AGAATCTTCT ACCCTTGGAC TTCCAGATTT TCCTTTAGAT
    AGTTTACCAG CTGCAGTTCA AATCCTGGAC TCCTTTCCTA TGATGTCAAT CCAACACGCG
    CTCCAGTGGC TTTACCCGTA CACTGTGTTA CTGGGCCATG AAGGAAAGAT GGCTGTGCAA
    GGGGTTTTAA AACGCTTTGC ACTCGAGGAT TCAAGAAGAT CTGTGCTTCC TGAGAAGATT
    GTGAGAGTGG AAAGGACGAC CGAAAACAAT GTCTTCCAAG CTTCTGTGAC CATCCGGATT
    GCAGAAAAAG AGGTGACCAT CAAGGTACCA GCTGGGACTA GGCCATTAAG TCAGCCTTGT
    ACATCGGACC GTTTTATACA AACCTTGAGC CATAAACAAC TACTTGCTGA AATGATGCAG
    TCTCACATGG TTAAGGACAT ATGCTTAATT GGAGGAAAAG GTTGTGGAAA AACAGTCATC
    GCTAAGAACT TTGCAGACAC CTTGGGATAC AATATAGAAC CTATCATGCT GTATCAGGAT
    ATGACAGCAC GTGATCTGCT ACAGCAGAGG TACACGCTTC CCAATGGAGA CACTGCCTGG
    CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG GAAGGAAAGC TGGTTCTCCT GGATGGCATT
    CACAGAGTCA ATGCCGGGAC ACTTGCTGTG TTACAGAGGC TGATCCATGA TCGGGAGCTA
    AGCCTCTATG ATGGCTCCAG GCTGCTGAGA GAAGACAGGT ACTTGCGCTT GAAGGAGGAG
    CTGCAAATGT CTGATGAGCA GCTGCAGCAG AGATCGATTT TTCCTGTTCA CCCCTCCTTC
    AGAATCATCG CCTTGGCAGA ACCCCCTGTT ATTGGAAGCA CGACACAGCA GTGGCTGGGG
    CCAGAATTCT TAACCATGTT CTTTTTCCAT TACATGAAAC CACTTGTCAA AAGTGAAGAA
    ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT ATGCCTCCGG AAGCTTTGGA CAAATTGTTG
    TCATTTACAC ACAAACTCAG AGAGACACAA GATCCAACAG CGCAGTCACT GGCGGCGTCC
    CTTTCTACCA GACAGCTGTT GAGGATTTCT CGTCGGCTGT CACAGTACCC TAATGAGAAC
    CTTCACAATG CTGTCACCAA AGCCTGTCTT TCCAGGTTTT TACCAAGCCT TGCAAGGTCA
    GCATTAGAAA AAAATCTGGC AGATGCTGCA ATAGAAATAA CCACTGATAA TAGTCCAGAG
    TCTGAACTGG AGGATTACAA ATGTGAACTC TCTTCTGGAT CTCTGAGGAT TGGCACGGTT
    AGTGCACCAA TCTATAATGC CCACGAGAAA ATGAAAGTGC CTGATGTTTT GTTCTATGAC
    AACATCCAGC ACATGATAGT GATGGAAGAT ATGCTCAAAG ACTTCCTTCT TGGAGAACAC
    CTGTTATTGG TCGGAAACCA GGGTGTAGGA AAAAACAAGA TTGTTGATAG ATTCCTTCAC
    CTGCTCAACA GACCCCGAGA GTATATCCAG CTGCACAGGG ACACCACAGT CCAAACACTT
    ACTCTTCAGC CTTCTGTTAA AGATGGACTT ATTGTATATG AAGACTCACC TTTGGTTAAA
    GCAGTAAAAT TGGGTCATGT TCTGGTAGTA GATGAGGCAG ACAAGGCTCC AACAAATGTC
    ACCTGTATTT TAAAAACTCT GGTAGAAAAT GGAGAAATGA TTCTAGCAGA TGGACGACGC
    ATTGTTGCAA ATGCTGCACT AGTCAATGGA AGAGAAAATG TTGTAGTGAT TCATCCTGAT
    TTTAGAATGA TTGTTCTGGC CAACAGACCT GGATTTCCTT TCCTAGGCAA TGATTTCTTC
    GGTACCTTAG GTGATATTTT TAGCTGCCAT GCAGTGGATA ATCCCAAACC CTACTCGGAA
    CTCAGGATGC TCAGGCAGTA CGGCCCAAAT GTGCCCGAGC CCATCCTTCA GAAGCTCGTC
    GCTGCCTTTG GAGAGCTGAG GAATTTAGCT GACCAGGGTA TTATTAACTA CCCTTATTCT
    ACCAGAGAAG TTGTCAACAT AGTCAAGCAT TTGCAGAAAT TTCCCACTGA AGGTCTCTCC
    AGTGTGGTTA GGAACGTGTT TGACTTTGAT TCCTACAACA ACGACATGAG GGAGATTTTG
    ACTAACACTT TGCACAAATA CGGAATACCA GTTGGAGCAA AACCATCCAA TGTGCAGCTG
    GCGAAGGAGT TGCCTCTGCC GGAGCAGACC TTCATGGGCT ACTGGACAAT TGGTCAGTCA
    AGAAATGGAA TGCAAAAACT GCTGTGTCCA GCAGAAACTC ATCATATAGA CGTTAAGGGT
    CCAGCACTCA TACACATACA GGAGTATCCA ATAGAAAGAC ATGAAGGAAG ATCCCAAACC
    TTTACCGAAG AATGTAGCTC CTGGAGGTTA CCATTGGATG AAATGAACTT AATCTGTGAT
    GTCGCTACAG CACATGAAAA TGAGGAAAAT ACTCTCTATG TAGTTGCATG CAATCCCGTG
    TCCTTATACT TTATGAATAT GACTGGGAAA AGCGGCCACT TTGTGGACCT TTTCGACATC
    TTCCCGAGAA CGGCCAGTGG AATCTGGCAC CCATTTGTGA CGGTGGCACC GCTGGGAAAC
    CCCCTAAAGG GTCAAGTGGT TCTCCACGAG CAGCAGAGCA ATGTCATCCT GTTGCTGGAT
    CCCGCTAGCC AAGCCCTTCG CCGTCTCATC CTACCTTCAG AAGAATTTAC AATGAAGAAA
    ACTTCCTGGT GGAACAAGGA GGAAGCTGAA ACTTACAAAA TGTGTAAAGA GTTTTCACAC
    AAAAACTGGC TAGTGTTCTA CAAAGAAAAA GGGAACACCC TCGCTGTGCT GGATGTTCTA
    GAAGGGCGAA CTCACACCAT CTCGCTTCCC ATCAACCTCC AGACGGTTTT TCTCGTAGCA
    GAAGACAAAT GGCTTCTGGT GGAGAGTAAA ACAAATCAGA AATATCTTTT AACTACACCT
    GCACACATCG AATCTAAGGA CAGTGGGGTT TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT
    CCTAGCCCAG GGTTTGGAGT CATGCAAGAA ACAGACCGCA GCATACCTCA TACAATTTCA
    AGTGATCAAC TGTCATCTGA AAATCTGAGT TCAGCTGTGG GACAAAAGAT TTCCTCTCCA
    AACCGAATTC TCTCAGATAA GAATAGTTAT GCTACAGTTG TTGTTGGTTT CCCAGATCTC
    ATGTCACCTA GTGAAGTTTA TTCTTGGAAA AGGCCATCAT CTTTGAGTAA ATCAAGAGTC
    ACTGATACAA CATTCTATGG AGGAAAGAAG AAAAGTGGAA CCGCGAAACA GAGCAATTGT
    GTGACTCTTT TGGATACTAA TCAGATAGTC AGGATTTTGC CCCCAGGAGA AGTCCCTCTA
    CAAGACATCT ACCCAAAAGA TGTTACCCCT CCACAGACAG CTGGGTATAT AGAAGTTACC
    GATCTCCAGT CCAAGAGACT TCGATACATC CCAATTCCCA GGTCAGACTC TCTCTCACCA
    TATACCACAT GGCTGTCTAC TATCTCGGAC ACAGACGCAC TGCTGGCCGA GTGGGGCAGA
    GGTGGCGTTG TCACTGTTGA TATGGGAGGC CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG
    CACCTGCAGC GGTCACTCAT GGAATGGAGA AACATGATTG GAGAAGGTGA CAGACACATG
    CAGATAACAA TCGAAAGAGA GAGCGGTGAA GACGTGAGTT CCCCCAAACA CGGGAAGGAG
    GACCCAGACA ACATGCCACA TGTGGGTGGT AACACTTGGG CTGGCGGCAC AGGTGGAAGA
    GACACCGCAG GCCTGGGCGG CAAGGGAGGT CCTTACCGGC TGGATGCAGG CCACACGGTC
    TACCAGGTCT CTGAGGCCGA GAAAGACGCT GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA
    GAGATGGGCC AGAGAGCGTT TCAACAGAGG CTAAAGGAGA TCCAAATGAG TGAATATGAT
    GCTGCCACCT ATGAAAGGTT TTCAGGGGCT GTTCGACGAC AGGTGCACTC CCTCCGAATT
    ATCCTGGATA ATTTACAGGC TAAAGGTAAA GAAAGACAGT GGCTAAGACA CCAGGCTACT
    GGGGAGCTAG ATGATGCCAA GATCATTGAT GGGCTGACTG GAGAAAAAGC CATCTACAAA
    CGCCGGGGTG AGCTGGAGCC ACAGATGGGC AGCCCCCAAC AGAAACCCAA GCGCCTGCGC
    CTGGTAGTAG ACGTGTCGGG CAGCATGTAC CGCTTCAACG GGGTGGATGG CCGGCTCGAG
    CGCTCGATGG AAGCTGTGTG CATGGTCATG GAGGCCTTCG AGAACTATGA GGAGAAGTTT
    AAGTATGACG TCGCTGGACA CTCTGGAGAT GGATACAACA TTGGTCTAGT TCCAATTAAC
    AAAATCCCCA AGGACAATAA GCAAAGACTA GAAATTCTCA AGACGATGCA CGCCCACGCT
    CAGTTCTGCA TGAGTGGGGA CCACACCTTA GAGGGGACAG AACATGCCAT CAAGGAAATC
    GTCAAAGAAG AAGCTGATGA GTACTTCGTC ATTGTCTTAA GTGATGCCAA CCTGTCTCGC
    TATGGAATAC ACCCCGCCAA GTTTGCTCAG ATCCTGACAA GCAGCCCTCA AGTAAACGCT
    TTCGCCATCT TTATTGGATC TTTAGGCGAT CAAGCAGCCC GGCTTCAGAG AACTCTACCA
    GCAGGCCGGT CTTTTGTTGC CATGGACACC AAGGACATCC CTCAGATTTT ACAACAGATT
    TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855784.1
    CDS412..5841
    Translation

    Target ORF information:

    RefSeq Version XM_025000016.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCAGAAGG ATCTTTTGGG GCAAGACGTC TTTCTAATAG GACCTCCTGG GCCTCTTCGA 
    CGCTCTATTG CTATGCAGTA CTTGGAGCTG ACCAAAAAGG AGGCTGAATA CATTGCCTTG
    TCAAGAGACA CCACCGAAAC CGACCTCAAA CAGCGGCGAG AGATCCGGGC GGGCACGGCT
    TTTTACATCG ATCAGTGTGC AGTTCGTGCA GCCACAGAAG GTAGAACACT CATCCTGGAA
    GGCTTGGAAA AGGCGGAGAG GAACGTCCTG CCGGTTTTGA ACAACTTGCT GGAGAACAGG
    GAGATGCAGC TTGAGGATGG ACGCTTCCTG ATGTCTGCCG AGCGTTACGA CAAACTTCTC
    CAAGATCATA CCAAAACAGA ATTGGATGCT TGGAAGATTG TCCGAGTTAA TGAAAATTTC
    CGAGTCATTG CCTTGGGCTT GCCAGTGCCC AGGTACTCTG GGAATCCATT AGATCCCCCA
    CTCCGGTCTC GATTTCAAGC CAGAGATATT TATTATCTTC CCTTCAAGGA CCAACTTAAA
    CTGTTATATT CAGTTGGAGC CAATGTTCCT ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT
    GCCACCACTC TCTGTTCCCA AGAATCTTCT ACCCTTGGAC TTCCAGATTT TCCTTTAGAT
    AGTTTACCAG CTGCAGTTCA AATCCTGGAC TCCTTTCCTA TGATGTCAAT CCAACACGCG
    CTCCAGTGGC TTTACCCGTA CACTGTGTTA CTGGGCCATG AAGGAAAGAT GGCTGTGCAA
    GGGGTTTTAA AACGCTTTGC ACTCGAGGAT TCAAGAAGAT CTGTGCTTCC TGAGAAGATT
    GTGAGAGTGG AAAGGACGAC CGAAAACAAT GTCTTCCAAG CTTCTGTGAC CATCCGGATT
    GCAGAAAAAG AGGTGACCAT CAAGGTACCA GCTGGGACTA GGCCATTAAG TCAGCCTTGT
    ACATCGGACC GTTTTATACA AACCTTGAGC CATAAACAAC TACTTGCTGA AATGATGCAG
    TCTCACATGG TTAAGGACAT ATGCTTAATT GGAGGAAAAG GTTGTGGAAA AACAGTCATC
    GCTAAGAACT TTGCAGACAC CTTGGGATAC AATATAGAAC CTATCATGCT GTATCAGGAT
    ATGACAGCAC GTGATCTGCT ACAGCAGAGG TACACGCTTC CCAATGGAGA CACTGCCTGG
    CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG GAAGGAAAGC TGGTTCTCCT GGATGGCATT
    CACAGAGTCA ATGCCGGGAC ACTTGCTGTG TTACAGAGGC TGATCCATGA TCGGGAGCTA
    AGCCTCTATG ATGGCTCCAG GCTGCTGAGA GAAGACAGGT ACTTGCGCTT GAAGGAGGAG
    CTGCAAATGT CTGATGAGCA GCTGCAGCAG AGATCGATTT TTCCTGTTCA CCCCTCCTTC
    AGAATCATCG CCTTGGCAGA ACCCCCTGTT ATTGGAAGCA CGACACAGCA GTGGCTGGGG
    CCAGAATTCT TAACCATGTT CTTTTTCCAT TACATGAAAC CACTTGTCAA AAGTGAAGAA
    ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT ATGCCTCCGG AAGCTTTGGA CAAATTGTTG
    TCATTTACAC ACAAACTCAG AGAGACACAA GATCCAACAG CGCAGTCACT GGCGGCGTCC
    CTTTCTACCA GACAGCTGTT GAGGATTTCT CGTCGGCTGT CACAGTACCC TAATGAGAAC
    CTTCACAATG CTGTCACCAA AGCCTGTCTT TCCAGGTTTT TACCAAGCCT TGCAAGGTCA
    GCATTAGAAA AAAATCTGGC AGATGCTGCA ATAGAAATAA CCACTGATAA TAGTCCAGAG
    TCTGAACTGG AGGATTACAA ATGTGAACTC TCTTCTGGAT CTCTGAGGAT TGGCACGGTT
    AGTGCACCAA TCTATAATGC CCACGAGAAA ATGAAAGTGC CTGATGTTTT GTTCTATGAC
    AACATCCAGC ACATGATAGT GATGGAAGAT ATGCTCAAAG ACTTCCTTCT TGGAGAACAC
    CTGTTATTGG TCGGAAACCA GGGTGTAGGA AAAAACAAGA TTGTTGATAG ATTCCTTCAC
    CTGCTCAACA GACCCCGAGA GTATATCCAG CTGCACAGGG ACACCACAGT CCAAACACTT
    ACTCTTCAGC CTTCTGTTAA AGATGGACTT ATTGTATATG AAGACTCACC TTTGGTTAAA
    GCAGTAAAAT TGGGTCATGT TCTGGTAGTA GATGAGGCAG ACAAGGCTCC AACAAATGTC
    ACCTGTATTT TAAAAACTCT GGTAGAAAAT GGAGAAATGA TTCTAGCAGA TGGACGACGC
    ATTGTTGCAA ATGCTGCACT AGTCAATGGA AGAGAAAATG TTGTAGTGAT TCATCCTGAT
    TTTAGAATGA TTGTTCTGGC CAACAGACCT GGATTTCCTT TCCTAGGCAA TGATTTCTTC
    GGTACCTTAG GTGATATTTT TAGCTGCCAT GCAGTGGATA ATCCCAAACC CTACTCGGAA
    CTCAGGATGC TCAGGCAGTA CGGCCCAAAT GTGCCCGAGC CCATCCTTCA GAAGCTCGTC
    GCTGCCTTTG GAGAGCTGAG GAATTTAGCT GACCAGGGTA TTATTAACTA CCCTTATTCT
    ACCAGAGAAG TTGTCAACAT AGTCAAGCAT TTGCAGAAAT TTCCCACTGA AGGTCTCTCC
    AGTGTGGTTA GGAACGTGTT TGACTTTGAT TCCTACAACA ACGACATGAG GGAGATTTTG
    ACTAACACTT TGCACAAATA CGGAATACCA GTTGGAGCAA AACCATCCAA TGTGCAGCTG
    GCGAAGGAGT TGCCTCTGCC GGAGCAGACC TTCATGGGCT ACTGGACAAT TGGTCAGTCA
    AGAAATGGAA TGCAAAAACT GCTGTGTCCA GCAGAAACTC ATCATATAGA CGTTAAGGGT
    CCAGCACTCA TACACATACA GGAGTATCCA ATAGAAAGAC ATGAAGGAAG ATCCCAAACC
    TTTACCGAAG AATGTAGCTC CTGGAGGTTA CCATTGGATG AAATGAACTT AATCTGTGAT
    GTCGCTACAG CACATGAAAA TGAGGAAAAT ACTCTCTATG TAGTTGCATG CAATCCCGTG
    TCCTTATACT TTATGAATAT GACTGGGAAA AGCGGCCACT TTGTGGACCT TTTCGACATC
    TTCCCGAGAA CGGCCAGTGG AATCTGGCAC CCATTTGTGA CGGTGGCACC GCTGGGAAAC
    CCCCTAAAGG GTCAAGTGGT TCTCCACGAG CAGCAGAGCA ATGTCATCCT GTTGCTGGAT
    CCCGCTAGCC AAGCCCTTCG CCGTCTCATC CTACCTTCAG AAGAATTTAC AATGAAGAAA
    ACTTCCTGGT GGAACAAGGA GGAAGCTGAA ACTTACAAAA TGTGTAAAGA GTTTTCACAC
    AAAAACTGGC TAGTGTTCTA CAAAGAAAAA GGGAACACCC TCGCTGTGCT GGATGTTCTA
    GAAGGGCGAA CTCACACCAT CTCGCTTCCC ATCAACCTCC AGACGGTTTT TCTCGTAGCA
    GAAGACAAAT GGCTTCTGGT GGAGAGTAAA ACAAATCAGA AATATCTTTT AACTACACCT
    GCACACATCG AATCTAAGGA CAGTGGGGTT TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT
    CCTAGCCCAG GGTTTGGAGT CATGCAAGAA ACAGACCGCA GCATACCTCA TACAATTTCA
    AGTGATCAAC TGTCATCTGA AAATCTGAGT TCAGCTGTGG GACAAAAGAT TTCCTCTCCA
    AACCGAATTC TCTCAGATAA GAATAGTTAT GCTACAGTTG TTGTTGGTTT CCCAGATCTC
    ATGTCACCTA GTGAAGTTTA TTCTTGGAAA AGGCCATCAT CTTTGAGTAA ATCAAGAGTC
    ACTGATACAA CATTCTATGG AGGAAAGAAG AAAAGTGGAA CCGCGAAACA GAGCAATTGT
    GTGACTCTTT TGGATACTAA TCAGATAGTC AGGATTTTGC CCCCAGGAGA AGTCCCTCTA
    CAAGACATCT ACCCAAAAGA TGTTACCCCT CCACAGACAG CTGGGTATAT AGAAGTTACC
    GATCTCCAGT CCAAGAGACT TCGATACATC CCAATTCCCA GGTCAGACTC TCTCTCACCA
    TATACCACAT GGCTGTCTAC TATCTCGGAC ACAGACGCAC TGCTGGCCGA GTGGGGCAGA
    GGTGGCGTTG TCACTGTTGA TATGGGAGGC CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG
    CACCTGCAGC GGTCACTCAT GGAATGGAGA AACATGATTG GAGAAGGTGA CAGACACATG
    CAGATAACAA TCGAAAGAGA GAGCGGTGAA GACGTGAGTT CCCCCAAACA CGGGAAGGAG
    GACCCAGACA ACATGCCACA TGTGGGTGGT AACACTTGGG CTGGCGGCAC AGGTGGAAGA
    GACACCGCAG GCCTGGGCGG CAAGGGAGGT CCTTACCGGC TGGATGCAGG CCACACGGTC
    TACCAGGTCT CTGAGGCCGA GAAAGACGCT GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA
    GAGATGGGCC AGAGAGCGTT TCAACAGAGG CTAAAGGAGA TCCAAATGAG TGAATATGAT
    GCTGCCACCT ATGAAAGGTT TTCAGGGGCT GTTCGACGAC AGGTGCACTC CCTCCGAATT
    ATCCTGGATA ATTTACAGGC TAAAGGTAAA GAAAGACAGT GGCTAAGACA CCAGGCTACT
    GGGGAGCTAG ATGATGCCAA GATCATTGAT GGGCTGACTG GAGAAAAAGC CATCTACAAA
    CGCCGGGGTG AGCTGGAGCC ACAGATGGGC AGCCCCCAAC AGAAACCCAA GCGCCTGCGC
    CTGGTAGTAG ACGTGTCGGG CAGCATGTAC CGCTTCAACG GGGTGGATGG CCGGCTCGAG
    CGCTCGATGG AAGCTGTGTG CATGGTCATG GAGGCCTTCG AGAACTATGA GGAGAAGTTT
    AAGTATGACG TCGCTGGACA CTCTGGAGAT GGATACAACA TTGGTCTAGT TCCAATTAAC
    AAAATCCCCA AGGACAATAA GCAAAGACTA GAAATTCTCA AGACGATGCA CGCCCACGCT
    CAGTTCTGCA TGAGTGGGGA CCACACCTTA GAGGGGACAG AACATGCCAT CAAGGAAATC
    GTCAAAGAAG AAGCTGATGA GTACTTCGTC ATTGTCTTAA GTGATGCCAA CCTGTCTCGC
    TATGGAATAC ACCCCGCCAA GTTTGCTCAG ATCCTGACAA GCAGCCCTCA AGTAAACGCT
    TTCGCCATCT TTATTGGATC TTTAGGCGAT CAAGCAGCCC GGCTTCAGAG AACTCTACCA
    GCAGGCCGGT CTTTTGTTGC CATGGACACC AAGGACATCC CTCAGATTTT ACAACAGATT
    TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221226
    Clone ID Related Accession (Same CDS sequence) XM_025000016.1 , XM_025000017.1
    Accession Version XM_025000017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCAGAAGG ATCTTTTGGG GCAAGACGTC TTTCTAATAG GACCTCCTGG GCCTCTTCGA 
    CGCTCTATTG CTATGCAGTA CTTGGAGCTG ACCAAAAAGG AGGCTGAATA CATTGCCTTG
    TCAAGAGACA CCACCGAAAC CGACCTCAAA CAGCGGCGAG AGATCCGGGC GGGCACGGCT
    TTTTACATCG ATCAGTGTGC AGTTCGTGCA GCCACAGAAG GTAGAACACT CATCCTGGAA
    GGCTTGGAAA AGGCGGAGAG GAACGTCCTG CCGGTTTTGA ACAACTTGCT GGAGAACAGG
    GAGATGCAGC TTGAGGATGG ACGCTTCCTG ATGTCTGCCG AGCGTTACGA CAAACTTCTC
    CAAGATCATA CCAAAACAGA ATTGGATGCT TGGAAGATTG TCCGAGTTAA TGAAAATTTC
    CGAGTCATTG CCTTGGGCTT GCCAGTGCCC AGGTACTCTG GGAATCCATT AGATCCCCCA
    CTCCGGTCTC GATTTCAAGC CAGAGATATT TATTATCTTC CCTTCAAGGA CCAACTTAAA
    CTGTTATATT CAGTTGGAGC CAATGTTCCT ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT
    GCCACCACTC TCTGTTCCCA AGAATCTTCT ACCCTTGGAC TTCCAGATTT TCCTTTAGAT
    AGTTTACCAG CTGCAGTTCA AATCCTGGAC TCCTTTCCTA TGATGTCAAT CCAACACGCG
    CTCCAGTGGC TTTACCCGTA CACTGTGTTA CTGGGCCATG AAGGAAAGAT GGCTGTGCAA
    GGGGTTTTAA AACGCTTTGC ACTCGAGGAT TCAAGAAGAT CTGTGCTTCC TGAGAAGATT
    GTGAGAGTGG AAAGGACGAC CGAAAACAAT GTCTTCCAAG CTTCTGTGAC CATCCGGATT
    GCAGAAAAAG AGGTGACCAT CAAGGTACCA GCTGGGACTA GGCCATTAAG TCAGCCTTGT
    ACATCGGACC GTTTTATACA AACCTTGAGC CATAAACAAC TACTTGCTGA AATGATGCAG
    TCTCACATGG TTAAGGACAT ATGCTTAATT GGAGGAAAAG GTTGTGGAAA AACAGTCATC
    GCTAAGAACT TTGCAGACAC CTTGGGATAC AATATAGAAC CTATCATGCT GTATCAGGAT
    ATGACAGCAC GTGATCTGCT ACAGCAGAGG TACACGCTTC CCAATGGAGA CACTGCCTGG
    CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG GAAGGAAAGC TGGTTCTCCT GGATGGCATT
    CACAGAGTCA ATGCCGGGAC ACTTGCTGTG TTACAGAGGC TGATCCATGA TCGGGAGCTA
    AGCCTCTATG ATGGCTCCAG GCTGCTGAGA GAAGACAGGT ACTTGCGCTT GAAGGAGGAG
    CTGCAAATGT CTGATGAGCA GCTGCAGCAG AGATCGATTT TTCCTGTTCA CCCCTCCTTC
    AGAATCATCG CCTTGGCAGA ACCCCCTGTT ATTGGAAGCA CGACACAGCA GTGGCTGGGG
    CCAGAATTCT TAACCATGTT CTTTTTCCAT TACATGAAAC CACTTGTCAA AAGTGAAGAA
    ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT ATGCCTCCGG AAGCTTTGGA CAAATTGTTG
    TCATTTACAC ACAAACTCAG AGAGACACAA GATCCAACAG CGCAGTCACT GGCGGCGTCC
    CTTTCTACCA GACAGCTGTT GAGGATTTCT CGTCGGCTGT CACAGTACCC TAATGAGAAC
    CTTCACAATG CTGTCACCAA AGCCTGTCTT TCCAGGTTTT TACCAAGCCT TGCAAGGTCA
    GCATTAGAAA AAAATCTGGC AGATGCTGCA ATAGAAATAA CCACTGATAA TAGTCCAGAG
    TCTGAACTGG AGGATTACAA ATGTGAACTC TCTTCTGGAT CTCTGAGGAT TGGCACGGTT
    AGTGCACCAA TCTATAATGC CCACGAGAAA ATGAAAGTGC CTGATGTTTT GTTCTATGAC
    AACATCCAGC ACATGATAGT GATGGAAGAT ATGCTCAAAG ACTTCCTTCT TGGAGAACAC
    CTGTTATTGG TCGGAAACCA GGGTGTAGGA AAAAACAAGA TTGTTGATAG ATTCCTTCAC
    CTGCTCAACA GACCCCGAGA GTATATCCAG CTGCACAGGG ACACCACAGT CCAAACACTT
    ACTCTTCAGC CTTCTGTTAA AGATGGACTT ATTGTATATG AAGACTCACC TTTGGTTAAA
    GCAGTAAAAT TGGGTCATGT TCTGGTAGTA GATGAGGCAG ACAAGGCTCC AACAAATGTC
    ACCTGTATTT TAAAAACTCT GGTAGAAAAT GGAGAAATGA TTCTAGCAGA TGGACGACGC
    ATTGTTGCAA ATGCTGCACT AGTCAATGGA AGAGAAAATG TTGTAGTGAT TCATCCTGAT
    TTTAGAATGA TTGTTCTGGC CAACAGACCT GGATTTCCTT TCCTAGGCAA TGATTTCTTC
    GGTACCTTAG GTGATATTTT TAGCTGCCAT GCAGTGGATA ATCCCAAACC CTACTCGGAA
    CTCAGGATGC TCAGGCAGTA CGGCCCAAAT GTGCCCGAGC CCATCCTTCA GAAGCTCGTC
    GCTGCCTTTG GAGAGCTGAG GAATTTAGCT GACCAGGGTA TTATTAACTA CCCTTATTCT
    ACCAGAGAAG TTGTCAACAT AGTCAAGCAT TTGCAGAAAT TTCCCACTGA AGGTCTCTCC
    AGTGTGGTTA GGAACGTGTT TGACTTTGAT TCCTACAACA ACGACATGAG GGAGATTTTG
    ACTAACACTT TGCACAAATA CGGAATACCA GTTGGAGCAA AACCATCCAA TGTGCAGCTG
    GCGAAGGAGT TGCCTCTGCC GGAGCAGACC TTCATGGGCT ACTGGACAAT TGGTCAGTCA
    AGAAATGGAA TGCAAAAACT GCTGTGTCCA GCAGAAACTC ATCATATAGA CGTTAAGGGT
    CCAGCACTCA TACACATACA GGAGTATCCA ATAGAAAGAC ATGAAGGAAG ATCCCAAACC
    TTTACCGAAG AATGTAGCTC CTGGAGGTTA CCATTGGATG AAATGAACTT AATCTGTGAT
    GTCGCTACAG CACATGAAAA TGAGGAAAAT ACTCTCTATG TAGTTGCATG CAATCCCGTG
    TCCTTATACT TTATGAATAT GACTGGGAAA AGCGGCCACT TTGTGGACCT TTTCGACATC
    TTCCCGAGAA CGGCCAGTGG AATCTGGCAC CCATTTGTGA CGGTGGCACC GCTGGGAAAC
    CCCCTAAAGG GTCAAGTGGT TCTCCACGAG CAGCAGAGCA ATGTCATCCT GTTGCTGGAT
    CCCGCTAGCC AAGCCCTTCG CCGTCTCATC CTACCTTCAG AAGAATTTAC AATGAAGAAA
    ACTTCCTGGT GGAACAAGGA GGAAGCTGAA ACTTACAAAA TGTGTAAAGA GTTTTCACAC
    AAAAACTGGC TAGTGTTCTA CAAAGAAAAA GGGAACACCC TCGCTGTGCT GGATGTTCTA
    GAAGGGCGAA CTCACACCAT CTCGCTTCCC ATCAACCTCC AGACGGTTTT TCTCGTAGCA
    GAAGACAAAT GGCTTCTGGT GGAGAGTAAA ACAAATCAGA AATATCTTTT AACTACACCT
    GCACACATCG AATCTAAGGA CAGTGGGGTT TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT
    CCTAGCCCAG GGTTTGGAGT CATGCAAGAA ACAGACCGCA GCATACCTCA TACAATTTCA
    AGTGATCAAC TGTCATCTGA AAATCTGAGT TCAGCTGTGG GACAAAAGAT TTCCTCTCCA
    AACCGAATTC TCTCAGATAA GAATAGTTAT GCTACAGTTG TTGTTGGTTT CCCAGATCTC
    ATGTCACCTA GTGAAGTTTA TTCTTGGAAA AGGCCATCAT CTTTGAGTAA ATCAAGAGTC
    ACTGATACAA CATTCTATGG AGGAAAGAAG AAAAGTGGAA CCGCGAAACA GAGCAATTGT
    GTGACTCTTT TGGATACTAA TCAGATAGTC AGGATTTTGC CCCCAGGAGA AGTCCCTCTA
    CAAGACATCT ACCCAAAAGA TGTTACCCCT CCACAGACAG CTGGGTATAT AGAAGTTACC
    GATCTCCAGT CCAAGAGACT TCGATACATC CCAATTCCCA GGTCAGACTC TCTCTCACCA
    TATACCACAT GGCTGTCTAC TATCTCGGAC ACAGACGCAC TGCTGGCCGA GTGGGGCAGA
    GGTGGCGTTG TCACTGTTGA TATGGGAGGC CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG
    CACCTGCAGC GGTCACTCAT GGAATGGAGA AACATGATTG GAGAAGGTGA CAGACACATG
    CAGATAACAA TCGAAAGAGA GAGCGGTGAA GACGTGAGTT CCCCCAAACA CGGGAAGGAG
    GACCCAGACA ACATGCCACA TGTGGGTGGT AACACTTGGG CTGGCGGCAC AGGTGGAAGA
    GACACCGCAG GCCTGGGCGG CAAGGGAGGT CCTTACCGGC TGGATGCAGG CCACACGGTC
    TACCAGGTCT CTGAGGCCGA GAAAGACGCT GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA
    GAGATGGGCC AGAGAGCGTT TCAACAGAGG CTAAAGGAGA TCCAAATGAG TGAATATGAT
    GCTGCCACCT ATGAAAGGTT TTCAGGGGCT GTTCGACGAC AGGTGCACTC CCTCCGAATT
    ATCCTGGATA ATTTACAGGC TAAAGGTAAA GAAAGACAGT GGCTAAGACA CCAGGCTACT
    GGGGAGCTAG ATGATGCCAA GATCATTGAT GGGCTGACTG GAGAAAAAGC CATCTACAAA
    CGCCGGGGTG AGCTGGAGCC ACAGATGGGC AGCCCCCAAC AGAAACCCAA GCGCCTGCGC
    CTGGTAGTAG ACGTGTCGGG CAGCATGTAC CGCTTCAACG GGGTGGATGG CCGGCTCGAG
    CGCTCGATGG AAGCTGTGTG CATGGTCATG GAGGCCTTCG AGAACTATGA GGAGAAGTTT
    AAGTATGACG TCGCTGGACA CTCTGGAGAT GGATACAACA TTGGTCTAGT TCCAATTAAC
    AAAATCCCCA AGGACAATAA GCAAAGACTA GAAATTCTCA AGACGATGCA CGCCCACGCT
    CAGTTCTGCA TGAGTGGGGA CCACACCTTA GAGGGGACAG AACATGCCAT CAAGGAAATC
    GTCAAAGAAG AAGCTGATGA GTACTTCGTC ATTGTCTTAA GTGATGCCAA CCTGTCTCGC
    TATGGAATAC ACCCCGCCAA GTTTGCTCAG ATCCTGACAA GCAGCCCTCA AGTAAACGCT
    TTCGCCATCT TTATTGGATC TTTAGGCGAT CAAGCAGCCC GGCTTCAGAG AACTCTACCA
    GCAGGCCGGT CTTTTGTTGC CATGGACACC AAGGACATCC CTCAGATTTT ACAACAGATT
    TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855785.1
    CDS323..5752
    Translation

    Target ORF information:

    RefSeq Version XM_025000017.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCAGAAGG ATCTTTTGGG GCAAGACGTC TTTCTAATAG GACCTCCTGG GCCTCTTCGA 
    CGCTCTATTG CTATGCAGTA CTTGGAGCTG ACCAAAAAGG AGGCTGAATA CATTGCCTTG
    TCAAGAGACA CCACCGAAAC CGACCTCAAA CAGCGGCGAG AGATCCGGGC GGGCACGGCT
    TTTTACATCG ATCAGTGTGC AGTTCGTGCA GCCACAGAAG GTAGAACACT CATCCTGGAA
    GGCTTGGAAA AGGCGGAGAG GAACGTCCTG CCGGTTTTGA ACAACTTGCT GGAGAACAGG
    GAGATGCAGC TTGAGGATGG ACGCTTCCTG ATGTCTGCCG AGCGTTACGA CAAACTTCTC
    CAAGATCATA CCAAAACAGA ATTGGATGCT TGGAAGATTG TCCGAGTTAA TGAAAATTTC
    CGAGTCATTG CCTTGGGCTT GCCAGTGCCC AGGTACTCTG GGAATCCATT AGATCCCCCA
    CTCCGGTCTC GATTTCAAGC CAGAGATATT TATTATCTTC CCTTCAAGGA CCAACTTAAA
    CTGTTATATT CAGTTGGAGC CAATGTTCCT ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT
    GCCACCACTC TCTGTTCCCA AGAATCTTCT ACCCTTGGAC TTCCAGATTT TCCTTTAGAT
    AGTTTACCAG CTGCAGTTCA AATCCTGGAC TCCTTTCCTA TGATGTCAAT CCAACACGCG
    CTCCAGTGGC TTTACCCGTA CACTGTGTTA CTGGGCCATG AAGGAAAGAT GGCTGTGCAA
    GGGGTTTTAA AACGCTTTGC ACTCGAGGAT TCAAGAAGAT CTGTGCTTCC TGAGAAGATT
    GTGAGAGTGG AAAGGACGAC CGAAAACAAT GTCTTCCAAG CTTCTGTGAC CATCCGGATT
    GCAGAAAAAG AGGTGACCAT CAAGGTACCA GCTGGGACTA GGCCATTAAG TCAGCCTTGT
    ACATCGGACC GTTTTATACA AACCTTGAGC CATAAACAAC TACTTGCTGA AATGATGCAG
    TCTCACATGG TTAAGGACAT ATGCTTAATT GGAGGAAAAG GTTGTGGAAA AACAGTCATC
    GCTAAGAACT TTGCAGACAC CTTGGGATAC AATATAGAAC CTATCATGCT GTATCAGGAT
    ATGACAGCAC GTGATCTGCT ACAGCAGAGG TACACGCTTC CCAATGGAGA CACTGCCTGG
    CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG GAAGGAAAGC TGGTTCTCCT GGATGGCATT
    CACAGAGTCA ATGCCGGGAC ACTTGCTGTG TTACAGAGGC TGATCCATGA TCGGGAGCTA
    AGCCTCTATG ATGGCTCCAG GCTGCTGAGA GAAGACAGGT ACTTGCGCTT GAAGGAGGAG
    CTGCAAATGT CTGATGAGCA GCTGCAGCAG AGATCGATTT TTCCTGTTCA CCCCTCCTTC
    AGAATCATCG CCTTGGCAGA ACCCCCTGTT ATTGGAAGCA CGACACAGCA GTGGCTGGGG
    CCAGAATTCT TAACCATGTT CTTTTTCCAT TACATGAAAC CACTTGTCAA AAGTGAAGAA
    ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT ATGCCTCCGG AAGCTTTGGA CAAATTGTTG
    TCATTTACAC ACAAACTCAG AGAGACACAA GATCCAACAG CGCAGTCACT GGCGGCGTCC
    CTTTCTACCA GACAGCTGTT GAGGATTTCT CGTCGGCTGT CACAGTACCC TAATGAGAAC
    CTTCACAATG CTGTCACCAA AGCCTGTCTT TCCAGGTTTT TACCAAGCCT TGCAAGGTCA
    GCATTAGAAA AAAATCTGGC AGATGCTGCA ATAGAAATAA CCACTGATAA TAGTCCAGAG
    TCTGAACTGG AGGATTACAA ATGTGAACTC TCTTCTGGAT CTCTGAGGAT TGGCACGGTT
    AGTGCACCAA TCTATAATGC CCACGAGAAA ATGAAAGTGC CTGATGTTTT GTTCTATGAC
    AACATCCAGC ACATGATAGT GATGGAAGAT ATGCTCAAAG ACTTCCTTCT TGGAGAACAC
    CTGTTATTGG TCGGAAACCA GGGTGTAGGA AAAAACAAGA TTGTTGATAG ATTCCTTCAC
    CTGCTCAACA GACCCCGAGA GTATATCCAG CTGCACAGGG ACACCACAGT CCAAACACTT
    ACTCTTCAGC CTTCTGTTAA AGATGGACTT ATTGTATATG AAGACTCACC TTTGGTTAAA
    GCAGTAAAAT TGGGTCATGT TCTGGTAGTA GATGAGGCAG ACAAGGCTCC AACAAATGTC
    ACCTGTATTT TAAAAACTCT GGTAGAAAAT GGAGAAATGA TTCTAGCAGA TGGACGACGC
    ATTGTTGCAA ATGCTGCACT AGTCAATGGA AGAGAAAATG TTGTAGTGAT TCATCCTGAT
    TTTAGAATGA TTGTTCTGGC CAACAGACCT GGATTTCCTT TCCTAGGCAA TGATTTCTTC
    GGTACCTTAG GTGATATTTT TAGCTGCCAT GCAGTGGATA ATCCCAAACC CTACTCGGAA
    CTCAGGATGC TCAGGCAGTA CGGCCCAAAT GTGCCCGAGC CCATCCTTCA GAAGCTCGTC
    GCTGCCTTTG GAGAGCTGAG GAATTTAGCT GACCAGGGTA TTATTAACTA CCCTTATTCT
    ACCAGAGAAG TTGTCAACAT AGTCAAGCAT TTGCAGAAAT TTCCCACTGA AGGTCTCTCC
    AGTGTGGTTA GGAACGTGTT TGACTTTGAT TCCTACAACA ACGACATGAG GGAGATTTTG
    ACTAACACTT TGCACAAATA CGGAATACCA GTTGGAGCAA AACCATCCAA TGTGCAGCTG
    GCGAAGGAGT TGCCTCTGCC GGAGCAGACC TTCATGGGCT ACTGGACAAT TGGTCAGTCA
    AGAAATGGAA TGCAAAAACT GCTGTGTCCA GCAGAAACTC ATCATATAGA CGTTAAGGGT
    CCAGCACTCA TACACATACA GGAGTATCCA ATAGAAAGAC ATGAAGGAAG ATCCCAAACC
    TTTACCGAAG AATGTAGCTC CTGGAGGTTA CCATTGGATG AAATGAACTT AATCTGTGAT
    GTCGCTACAG CACATGAAAA TGAGGAAAAT ACTCTCTATG TAGTTGCATG CAATCCCGTG
    TCCTTATACT TTATGAATAT GACTGGGAAA AGCGGCCACT TTGTGGACCT TTTCGACATC
    TTCCCGAGAA CGGCCAGTGG AATCTGGCAC CCATTTGTGA CGGTGGCACC GCTGGGAAAC
    CCCCTAAAGG GTCAAGTGGT TCTCCACGAG CAGCAGAGCA ATGTCATCCT GTTGCTGGAT
    CCCGCTAGCC AAGCCCTTCG CCGTCTCATC CTACCTTCAG AAGAATTTAC AATGAAGAAA
    ACTTCCTGGT GGAACAAGGA GGAAGCTGAA ACTTACAAAA TGTGTAAAGA GTTTTCACAC
    AAAAACTGGC TAGTGTTCTA CAAAGAAAAA GGGAACACCC TCGCTGTGCT GGATGTTCTA
    GAAGGGCGAA CTCACACCAT CTCGCTTCCC ATCAACCTCC AGACGGTTTT TCTCGTAGCA
    GAAGACAAAT GGCTTCTGGT GGAGAGTAAA ACAAATCAGA AATATCTTTT AACTACACCT
    GCACACATCG AATCTAAGGA CAGTGGGGTT TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT
    CCTAGCCCAG GGTTTGGAGT CATGCAAGAA ACAGACCGCA GCATACCTCA TACAATTTCA
    AGTGATCAAC TGTCATCTGA AAATCTGAGT TCAGCTGTGG GACAAAAGAT TTCCTCTCCA
    AACCGAATTC TCTCAGATAA GAATAGTTAT GCTACAGTTG TTGTTGGTTT CCCAGATCTC
    ATGTCACCTA GTGAAGTTTA TTCTTGGAAA AGGCCATCAT CTTTGAGTAA ATCAAGAGTC
    ACTGATACAA CATTCTATGG AGGAAAGAAG AAAAGTGGAA CCGCGAAACA GAGCAATTGT
    GTGACTCTTT TGGATACTAA TCAGATAGTC AGGATTTTGC CCCCAGGAGA AGTCCCTCTA
    CAAGACATCT ACCCAAAAGA TGTTACCCCT CCACAGACAG CTGGGTATAT AGAAGTTACC
    GATCTCCAGT CCAAGAGACT TCGATACATC CCAATTCCCA GGTCAGACTC TCTCTCACCA
    TATACCACAT GGCTGTCTAC TATCTCGGAC ACAGACGCAC TGCTGGCCGA GTGGGGCAGA
    GGTGGCGTTG TCACTGTTGA TATGGGAGGC CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG
    CACCTGCAGC GGTCACTCAT GGAATGGAGA AACATGATTG GAGAAGGTGA CAGACACATG
    CAGATAACAA TCGAAAGAGA GAGCGGTGAA GACGTGAGTT CCCCCAAACA CGGGAAGGAG
    GACCCAGACA ACATGCCACA TGTGGGTGGT AACACTTGGG CTGGCGGCAC AGGTGGAAGA
    GACACCGCAG GCCTGGGCGG CAAGGGAGGT CCTTACCGGC TGGATGCAGG CCACACGGTC
    TACCAGGTCT CTGAGGCCGA GAAAGACGCT GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA
    GAGATGGGCC AGAGAGCGTT TCAACAGAGG CTAAAGGAGA TCCAAATGAG TGAATATGAT
    GCTGCCACCT ATGAAAGGTT TTCAGGGGCT GTTCGACGAC AGGTGCACTC CCTCCGAATT
    ATCCTGGATA ATTTACAGGC TAAAGGTAAA GAAAGACAGT GGCTAAGACA CCAGGCTACT
    GGGGAGCTAG ATGATGCCAA GATCATTGAT GGGCTGACTG GAGAAAAAGC CATCTACAAA
    CGCCGGGGTG AGCTGGAGCC ACAGATGGGC AGCCCCCAAC AGAAACCCAA GCGCCTGCGC
    CTGGTAGTAG ACGTGTCGGG CAGCATGTAC CGCTTCAACG GGGTGGATGG CCGGCTCGAG
    CGCTCGATGG AAGCTGTGTG CATGGTCATG GAGGCCTTCG AGAACTATGA GGAGAAGTTT
    AAGTATGACG TCGCTGGACA CTCTGGAGAT GGATACAACA TTGGTCTAGT TCCAATTAAC
    AAAATCCCCA AGGACAATAA GCAAAGACTA GAAATTCTCA AGACGATGCA CGCCCACGCT
    CAGTTCTGCA TGAGTGGGGA CCACACCTTA GAGGGGACAG AACATGCCAT CAAGGAAATC
    GTCAAAGAAG AAGCTGATGA GTACTTCGTC ATTGTCTTAA GTGATGCCAA CCTGTCTCGC
    TATGGAATAC ACCCCGCCAA GTTTGCTCAG ATCCTGACAA GCAGCCCTCA AGTAAACGCT
    TTCGCCATCT TTATTGGATC TTTAGGCGAT CAAGCAGCCC GGCTTCAGAG AACTCTACCA
    GCAGGCCGGT CTTTTGTTGC CATGGACACC AAGGACATCC CTCAGATTTT ACAACAGATT
    TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221227
    Clone ID Related Accession (Same CDS sequence) XM_025000018.1
    Accession Version XM_025000018.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGAATCTCC TGGGCCTGAC TCAACGCCAT
    CTGAAAATGG AAGTGGGTAC TCTCAGCAAA CTATTCATAG AAACGGAAGA GAACTTCCTC
    AGCCTGAGAA AAGGCATCTG TAGCCTGACA GCTGGCACCA TACTTCATGG TGAAAGGCTG
    AGTGCTTTCC CTCTAAGACT GGGACTAAGA CAGGGACGAC CACAGCCGTG CACCCGTCAC
    TCCTGTTCAG CATCAGACTG GAAGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855786.1
    CDS71..4900
    Translation

    Target ORF information:

    RefSeq Version XM_025000018.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGAATCTCC TGGGCCTGAC TCAACGCCAT
    CTGAAAATGG AAGTGGGTAC TCTCAGCAAA CTATTCATAG AAACGGAAGA GAACTTCCTC
    AGCCTGAGAA AAGGCATCTG TAGCCTGACA GCTGGCACCA TACTTCATGG TGAAAGGCTG
    AGTGCTTTCC CTCTAAGACT GGGACTAAGA CAGGGACGAC CACAGCCGTG CACCCGTCAC
    TCCTGTTCAG CATCAGACTG GAAGTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221228
    Clone ID Related Accession (Same CDS sequence) XM_025000019.1
    Accession Version XM_025000019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGGTGGAAG AGACACCGCA GGCCTGGGCG
    GCAAGGGAGG TCCTTACCGG CTGGATGCAG GCCACACGGT CTACCAGGTC TCTGAGGCCG
    AGAAAGACGC TGTTCCCGAG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855787.1
    CDS70..4776
    Translation

    Target ORF information:

    RefSeq Version XM_025000019.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGGTGGAAG AGACACCGCA GGCCTGGGCG
    GCAAGGGAGG TCCTTACCGG CTGGATGCAG GCCACACGGT CTACCAGGTC TCTGAGGCCG
    AGAAAGACGC TGTTCCCGAG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221229
    Clone ID Related Accession (Same CDS sequence) XM_025000020.1
    Accession Version XM_025000020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGGAACTCC GAGGAAGCAA CGCGCTCCTT
    CATCAGTCTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855788.1
    CDS70..4704
    Translation

    Target ORF information:

    RefSeq Version XM_025000020.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAAAG TTTCTCAGCT TTTGTCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT
    ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAC
    TCCTTTCCTA TGATGTCAAT CCAACACGCG CTCCAGTGGC TTTACCCGTA CACTGTGTTA
    CTGGGCCATG AAGGAAAGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGC ACTCGAGGAT
    TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGACGAC CGAAAACAAT
    GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGGAACTCC GAGGAAGCAA CGCGCTCCTT
    CATCAGTCTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24744
    Clone ID Related Accession (Same CDS sequence) XM_002691823.4 , XM_015465667.1
    Accession Version XM_002691823.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691823.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAACC AGCTGCTGCA GGTCTTTGAA AGAAGACTGC CCTTGAGTTC CTGGAGCCAG
    CTTGGCTCCC AGGGTGGGAG AGCAGCTAGT GCCTGGGAAC ATGTGGCCCC GGGGACAACA
    CTAACCAGGG ACCGATGGCG CTGGAGTGTG AAAGCCCTAG CTCGCCTACC TCAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACG TCGCTGGACA CTCTGGAGAT
    GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA
    GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA
    GAGGGGACAG AACATGCCAT CAAGGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC
    ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCCGCCAA GTTTGCTCAG
    ATCCTGACAA GCAGCCCTCA AGTAAACGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT
    CAAGCAGCCC GGCTTCAGAG AACTCTACCA GCAGGCCGGT CTTTTGTTGC CATGGACACC
    AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691869.1
    CDS43..5562
    Translation

    Target ORF information:

    RefSeq Version XM_002691823.4
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691823.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAACC AGCTGCTGCA GGTCTTTGAA AGAAGACTGC CCTTGAGTTC CTGGAGCCAG
    CTTGGCTCCC AGGGTGGGAG AGCAGCTAGT GCCTGGGAAC ATGTGGCCCC GGGGACAACA
    CTAACCAGGG ACCGATGGCG CTGGAGTGTG AAAGCCCTAG CTCGCCTACC TCAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACG TCGCTGGACA CTCTGGAGAT
    GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA
    GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA
    GAGGGGACAG AACATGCCAT CAAGGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC
    ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCCGCCAA GTTTGCTCAG
    ATCCTGACAA GCAGCCCTCA AGTAAACGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT
    CAAGCAGCCC GGCTTCAGAG AACTCTACCA GCAGGCCGGT CTTTTGTTGC CATGGACACC
    AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24744
    Clone ID Related Accession (Same CDS sequence) XM_002691823.4 , XM_015465667.1
    Accession Version XM_015465667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product von Willebrand factor A domain-containing protein 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAACC AGCTGCTGCA GGTCTTTGAA AGAAGACTGC CCTTGAGTTC CTGGAGCCAG
    CTTGGCTCCC AGGGTGGGAG AGCAGCTAGT GCCTGGGAAC ATGTGGCCCC GGGGACAACA
    CTAACCAGGG ACCGATGGCG CTGGAGTGTG AAAGCCCTAG CTCGCCTACC TCAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACG TCGCTGGACA CTCTGGAGAT
    GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA
    GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA
    GAGGGGACAG AACATGCCAT CAAGGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC
    ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCCGCCAA GTTTGCTCAG
    ATCCTGACAA GCAGCCCTCA AGTAAACGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT
    CAAGCAGCCC GGCTTCAGAG AACTCTACCA GCAGGCCGGT CTTTTGTTGC CATGGACACC
    AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321153.1
    CDS43..5562
    Translation

    Target ORF information:

    RefSeq Version XM_015465667.1
    Organism Bos taurus(cattle)
    Definition Bos taurus von Willebrand factor A domain containing 8 (VWA8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCAGTCGC GGCTCCTGCT TCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCCTG 
    CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGATGC CAGGCTTGTG
    CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG
    TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATATCAGA CTCTCTGGCT
    CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC
    TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CTTGGAGCTG
    ACCAAAAAGG AGGCTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA
    CAGCGGCGAG AGATCCGGGC GGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA
    GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG
    CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG
    ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT
    TGGAAGATTG TCCGAGTTAA TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC
    AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT
    TATTATCTTC CCTTCAAGGA CCAACTTAAA CTGTTATATT CAGTTGGAGC CAATGTTCCT
    ACTGAAAACC AGCTGCTGCA GGTCTTTGAA AGAAGACTGC CCTTGAGTTC CTGGAGCCAG
    CTTGGCTCCC AGGGTGGGAG AGCAGCTAGT GCCTGGGAAC ATGTGGCCCC GGGGACAACA
    CTAACCAGGG ACCGATGGCG CTGGAGTGTG AAAGCCCTAG CTCGCCTACC TCAGGTACCA
    GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC
    CATAAACAAC TACTTGCTGA AATGATGCAG TCTCACATGG TTAAGGACAT ATGCTTAATT
    GGAGGAAAAG GTTGTGGAAA AACAGTCATC GCTAAGAACT TTGCAGACAC CTTGGGATAC
    AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCAC GTGATCTGCT ACAGCAGAGG
    TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG
    GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC ACTTGCTGTG
    TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTCTATG ATGGCTCCAG GCTGCTGAGA
    GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG
    AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT
    ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT
    TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT
    ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACAC ACAAACTCAG AGAGACACAA
    GATCCAACAG CGCAGTCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT
    CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT
    TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA
    ATAGAAATAA CCACTGATAA TAGTCCAGAG TCTGAACTGG AGGATTACAA ATGTGAACTC
    TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA
    ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT
    ATGCTCAAAG ACTTCCTTCT TGGAGAACAC CTGTTATTGG TCGGAAACCA GGGTGTAGGA
    AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG
    CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT
    ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA
    GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT GGTAGAAAAT
    GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA
    AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT
    GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT
    GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT
    GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAGCTGAG GAATTTAGCT
    GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT
    TTGCAGAAAT TTCCCACTGA AGGTCTCTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT
    TCCTACAACA ACGACATGAG GGAGATTTTG ACTAACACTT TGCACAAATA CGGAATACCA
    GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC
    TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA
    GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA
    ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA
    CCATTGGATG AAATGAACTT AATCTGTGAT GTCGCTACAG CACATGAAAA TGAGGAAAAT
    ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAA
    AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG AATCTGGCAC
    CCATTTGTGA CGGTGGCACC GCTGGGAAAC CCCCTAAAGG GTCAAGTGGT TCTCCACGAG
    CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC
    CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA
    ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA
    GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC
    ATCAACCTCC AGACGGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA
    ACAAATCAGA AATATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT
    TGCCAGTTGT ATGTGTTGCA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA
    ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT
    TCAGCTGTGG GACAAAAGAT TTCCTCTCCA AACCGAATTC TCTCAGATAA GAATAGTTAT
    GCTACAGTTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA
    AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG
    AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACTAA TCAGATAGTC
    AGGATTTTGC CCCCAGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT
    CCACAGACAG CTGGGTATAT AGAAGTTACC GATCTCCAGT CCAAGAGACT TCGATACATC
    CCAATTCCCA GGTCAGACTC TCTCTCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC
    ACAGACGCAC TGCTGGCCGA GTGGGGCAGA GGTGGCGTTG TCACTGTTGA TATGGGAGGC
    CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA
    AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA
    GACGTGAGTT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT
    AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACCGCAG GCCTGGGCGG CAAGGGAGGT
    CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTGAGGCCGA GAAAGACGCT
    GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG
    CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT
    GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA
    GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT
    GGGCTGACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAGATGGGC
    AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTAGTAG ACGTGTCGGG CAGCATGTAC
    CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCGATGG AAGCTGTGTG CATGGTCATG
    GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACG TCGCTGGACA CTCTGGAGAT
    GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA
    GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA
    GAGGGGACAG AACATGCCAT CAAGGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC
    ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCCGCCAA GTTTGCTCAG
    ATCCTGACAA GCAGCCCTCA AGTAAACGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT
    CAAGCAGCCC GGCTTCAGAG AACTCTACCA GCAGGCCGGT CTTTTGTTGC CATGGACACC
    AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.