MIDN gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005209325.3	XP_005209382.1	midnolin isoform X1
XM_002689146.5	XP_002689192.1	midnolin isoform X3
XM_005199026.3	XP_005199083.1	midnolin isoform X2
XM_866159.7	XP_871252.2	midnolin isoform X3
XM_005209326.4	XP_005209383.2	midnolin isoform X2
XM_005209326.3	XP_005209383.1	midnolin isoform X2
XM_002689146.6	XP_002689192.2	midnolin isoform X3
XM_010807280.1	XP_010805582.1	midnolin isoform X1
XM_015464254.1	XP_015319740.1	midnolin isoform X1
XM_005199025.3	XP_005199082.1	midnolin isoform X1
XM_005209325.4	XP_005209382.2	midnolin isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236651	XM_005209325.4 Latest version!	Bos taurus midnolin (MIDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa236652	XM_005209326.4 Latest version!	Bos taurus midnolin (MIDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa236653	XM_002689146.6 Latest version!	Bos taurus midnolin (MIDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa05458	XM_015464254.1 Latest version!	Bos taurus midnolin (MIDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa05458	XM_005199025.3 Latest version!	Bos taurus midnolin (MIDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa05458	XM_005209325.3 Latest version!	Bos taurus midnolin (MIDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa05458	XM_010807280.1 Latest version!	Bos taurus midnolin (MIDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa05459	XM_005199026.3 Latest version!	Bos taurus midnolin (MIDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa05459	XM_005209326.3 Latest version!	Bos taurus midnolin (MIDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa05460	XM_866159.7 Latest version!	Bos taurus midnolin (MIDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa05460	XM_002689146.5 Latest version!	Bos taurus midnolin (MIDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

MIDN ( XM_005209325.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209382.2 Bos taurus midnolin (MIDN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236651
Clone ID Related Accession (Same CDS sequence)	XM_005209325.4
Accession Version	XM_005209325.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	midnolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209325.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT ACCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209382.2
CDS	540..2030
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPTPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_005209325.4
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209325.4

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT ACCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_005209326.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209383.2 Bos taurus midnolin (MIDN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236652
Clone ID Related Accession (Same CDS sequence)	XM_005209326.4
Accession Version	XM_005209326.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	midnolin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209326.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT ACCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGA TGGACTGTTC CCCCGCTGCC 
AGCACTGGTG CCAGCCCCAC ATCCCCGACT GGGAGCAGCC CCACCTCCCG CTCCCGCAAA 
CCTGGCGCAG TCATCGAGAG CTTCGTGAAC CACGCCCCGG GGGTCTTCTC AGGGACCTTC 
TCTGGCACAC TGCACCCCAA CTGCCAGGAC AGCAGTGGGC GGCCGAGGCG GGACATCGGG 
ACCATCCTGC AGATCCTCAA TGACCTCCTA AGCGCCACAC GGCACTACCA GGGCATGCCC 
CCATCACTCA CACAGCTGCG TTGCCACGCC CAGTGCACCC CAGACCTCGG CCCCAAAACT 
ACCTCCTGTG AGAAGCTGGC GGCCGCGGCC CCGGCCTCCC TGAGCCAGGC CCGCTTGTGC 
AAGCCCCCGG GGGACCGGCT GCGGCAGACG GAGAACCGAG CCACCCGCTG TAAGGTGGAG 
CGCCTTCAGC TGCTGCTCCA GCAGAAACGG CTCCGCAGAA AGGCCCGGCG TGATGCCCGC 
GGTCCCTACC ACTGGCCGCC CAGCCGCAAG GCCGGCCGCA GCGACAGCAC TGGCAGTGGG 
GGAGGTGGCA GCCCCAGCGA GGCGGCCGGC TTGGGCCTTG ACTTCGAGGA CTCCGTCTGG 
AAGCCAGAAG TGAACCCCGA CATCAAATCA GAGTTTGTGG TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209383.2
CDS	540..2027
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPTPASSPVTAGSFRSHSASTTCPEMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_005209326.4
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209326.4

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT ACCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGA TGGACTGTTC CCCCGCTGCC 
AGCACTGGTG CCAGCCCCAC ATCCCCGACT GGGAGCAGCC CCACCTCCCG CTCCCGCAAA 
CCTGGCGCAG TCATCGAGAG CTTCGTGAAC CACGCCCCGG GGGTCTTCTC AGGGACCTTC 
TCTGGCACAC TGCACCCCAA CTGCCAGGAC AGCAGTGGGC GGCCGAGGCG GGACATCGGG 
ACCATCCTGC AGATCCTCAA TGACCTCCTA AGCGCCACAC GGCACTACCA GGGCATGCCC 
CCATCACTCA CACAGCTGCG TTGCCACGCC CAGTGCACCC CAGACCTCGG CCCCAAAACT 
ACCTCCTGTG AGAAGCTGGC GGCCGCGGCC CCGGCCTCCC TGAGCCAGGC CCGCTTGTGC 
AAGCCCCCGG GGGACCGGCT GCGGCAGACG GAGAACCGAG CCACCCGCTG TAAGGTGGAG 
CGCCTTCAGC TGCTGCTCCA GCAGAAACGG CTCCGCAGAA AGGCCCGGCG TGATGCCCGC 
GGTCCCTACC ACTGGCCGCC CAGCCGCAAG GCCGGCCGCA GCGACAGCAC TGGCAGTGGG 
GGAGGTGGCA GCCCCAGCGA GGCGGCCGGC TTGGGCCTTG ACTTCGAGGA CTCCGTCTGG 
AAGCCAGAAG TGAACCCCGA CATCAAATCA GAGTTTGTGG TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_002689146.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689192.2 Bos taurus midnolin (MIDN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa236653
Clone ID Related Accession (Same CDS sequence)	XM_002689146.6
Accession Version	XM_002689146.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	midnolin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689146.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGGTCAGT GACTTCTTGT CAGGACGCTC GCCGCTGACC 
CTGGCGCTGC GTGTGGGGGA CCACATGATG TTTGTCCAGT TGCAGCTCGC AGCCCAGCAT 
GCCCCGCTGC AGCACCGCCA CGTGCTGGCT GCTGCTGCTG CCGCCACCCG CGGGGACCCC 
AGCATGACCA CCACCCCTGT GTCCTCTCCC TGCCGGCCAG TGTCCAGTGC TGCCCGTGTC 
CCCCCAGTGC CCACCAGCCC TACCCCCGCA TCTTCACCTG TCACAGCCGG CTCTTTCCGC 
TCCCATTCAG CCTCTACCAC CTGCCCCGAG CAGATGGACT GTTCCCCCGC TGCCAGCACT 
GGTGCCAGCC CCACATCCCC GACTGGGAGC AGCCCCACCT CCCGCTCCCG CAAACCTGGC 
GCAGTCATCG AGAGCTTCGT GAACCACGCC CCGGGGGTCT TCTCAGGGAC CTTCTCTGGC 
ACACTGCACC CCAACTGCCA GGACAGCAGT GGGCGGCCGA GGCGGGACAT CGGGACCATC 
CTGCAGATCC TCAATGACCT CCTAAGCGCC ACACGGCACT ACCAGGGCAT GCCCCCATCA 
CTCACACAGC TGCGTTGCCA CGCCCAGTGC ACCCCAGACC TCGGCCCCAA AACTACCTCC 
TGTGAGAAGC TGGCGGCCGC GGCCCCGGCC TCCCTGAGCC AGGCCCGCTT GTGCAAGCCC 
CCGGGGGACC GGCTGCGGCA GACGGAGAAC CGAGCCACCC GCTGTAAGGT GGAGCGCCTT 
CAGCTGCTGC TCCAGCAGAA ACGGCTCCGC AGAAAGGCCC GGCGTGATGC CCGCGGTCCC 
TACCACTGGC CGCCCAGCCG CAAGGCCGGC CGCAGCGACA GCACTGGCAG TGGGGGAGGT 
GGCAGCCCCA GCGAGGCGGC CGGCTTGGGC CTTGACTTCG AGGACTCCGT CTGGAAGCCA 
GAAGTGAACC CCGACATCAA ATCAGAGTTT GTGGTGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689192.2
CDS	539..1900
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPTPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_002689146.6
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689146.6

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGGTCAGT GACTTCTTGT CAGGACGCTC GCCGCTGACC 
CTGGCGCTGC GTGTGGGGGA CCACATGATG TTTGTCCAGT TGCAGCTCGC AGCCCAGCAT 
GCCCCGCTGC AGCACCGCCA CGTGCTGGCT GCTGCTGCTG CCGCCACCCG CGGGGACCCC 
AGCATGACCA CCACCCCTGT GTCCTCTCCC TGCCGGCCAG TGTCCAGTGC TGCCCGTGTC 
CCCCCAGTGC CCACCAGCCC TACCCCCGCA TCTTCACCTG TCACAGCCGG CTCTTTCCGC 
TCCCATTCAG CCTCTACCAC CTGCCCCGAG CAGATGGACT GTTCCCCCGC TGCCAGCACT 
GGTGCCAGCC CCACATCCCC GACTGGGAGC AGCCCCACCT CCCGCTCCCG CAAACCTGGC 
GCAGTCATCG AGAGCTTCGT GAACCACGCC CCGGGGGTCT TCTCAGGGAC CTTCTCTGGC 
ACACTGCACC CCAACTGCCA GGACAGCAGT GGGCGGCCGA GGCGGGACAT CGGGACCATC 
CTGCAGATCC TCAATGACCT CCTAAGCGCC ACACGGCACT ACCAGGGCAT GCCCCCATCA 
CTCACACAGC TGCGTTGCCA CGCCCAGTGC ACCCCAGACC TCGGCCCCAA AACTACCTCC 
TGTGAGAAGC TGGCGGCCGC GGCCCCGGCC TCCCTGAGCC AGGCCCGCTT GTGCAAGCCC 
CCGGGGGACC GGCTGCGGCA GACGGAGAAC CGAGCCACCC GCTGTAAGGT GGAGCGCCTT 
CAGCTGCTGC TCCAGCAGAA ACGGCTCCGC AGAAAGGCCC GGCGTGATGC CCGCGGTCCC 
TACCACTGGC CGCCCAGCCG CAAGGCCGGC CGCAGCGACA GCACTGGCAG TGGGGGAGGT 
GGCAGCCCCA GCGAGGCGGC CGGCTTGGGC CTTGACTTCG AGGACTCCGT CTGGAAGCCA 
GAAGTGAACC CCGACATCAA ATCAGAGTTT GTGGTGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_005209325.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209382.1 Bos taurus midnolin (MIDN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa05458
Clone ID Related Accession (Same CDS sequence)	XM_005209325.3 , XM_010807280.1 , XM_015464254.1 , XM_005199025.3
Accession Version	XM_005209325.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209382.1
CDS	507..1997
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_005209325.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209325.3

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_010807280.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805582.1 Bos taurus midnolin (MIDN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa05458
Clone ID Related Accession (Same CDS sequence)	XM_005209325.3 , XM_010807280.1 , XM_015464254.1 , XM_005199025.3
Accession Version	XM_010807280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805582.1
CDS	638..2128
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_010807280.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807280.1

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_015464254.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319740.1 Bos taurus midnolin (MIDN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa05458
Clone ID Related Accession (Same CDS sequence)	XM_005209325.3 , XM_010807280.1 , XM_015464254.1 , XM_005199025.3
Accession Version	XM_015464254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319740.1
CDS	638..2128
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_015464254.1
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464254.1

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_005199025.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005199082.1 Bos taurus midnolin (MIDN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa05458
Clone ID Related Accession (Same CDS sequence)	XM_005209325.3 , XM_010807280.1 , XM_015464254.1 , XM_005199025.3
Accession Version	XM_005199025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005199082.1
CDS	507..1997
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_005199025.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005199025.3

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGC AGATGGACTG TTCCCCCGCT 
GCCAGCACTG GTGCCAGCCC CACATCCCCG ACTGGGAGCA GCCCCACCTC CCGCTCCCGC 
AAACCTGGCG CAGTCATCGA GAGCTTCGTG AACCACGCCC CGGGGGTCTT CTCAGGGACC 
TTCTCTGGCA CACTGCACCC CAACTGCCAG GACAGCAGTG GGCGGCCGAG GCGGGACATC 
GGGACCATCC TGCAGATCCT CAATGACCTC CTAAGCGCCA CACGGCACTA CCAGGGCATG 
CCCCCATCAC TCACACAGCT GCGTTGCCAC GCCCAGTGCA CCCCAGACCT CGGCCCCAAA 
ACTACCTCCT GTGAGAAGCT GGCGGCCGCG GCCCCGGCCT CCCTGAGCCA GGCCCGCTTG 
TGCAAGCCCC CGGGGGACCG GCTGCGGCAG ACGGAGAACC GAGCCACCCG CTGTAAGGTG 
GAGCGCCTTC AGCTGCTGCT CCAGCAGAAA CGGCTCCGCA GAAAGGCCCG GCGTGATGCC 
CGCGGTCCCT ACCACTGGCC GCCCAGCCGC AAGGCCGGCC GCAGCGACAG CACTGGCAGT 
GGGGGAGGTG GCAGCCCCAG CGAGGCGGCC GGCTTGGGCC TTGACTTCGA GGACTCCGTC 
TGGAAGCCAG AAGTGAACCC CGACATCAAA TCAGAGTTTG TGGTGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_005199026.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005199083.1 Bos taurus midnolin (MIDN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa05459
Clone ID Related Accession (Same CDS sequence)	XM_005199026.3 , XM_005209326.3
Accession Version	XM_005199026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199026.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGA TGGACTGTTC CCCCGCTGCC 
AGCACTGGTG CCAGCCCCAC ATCCCCGACT GGGAGCAGCC CCACCTCCCG CTCCCGCAAA 
CCTGGCGCAG TCATCGAGAG CTTCGTGAAC CACGCCCCGG GGGTCTTCTC AGGGACCTTC 
TCTGGCACAC TGCACCCCAA CTGCCAGGAC AGCAGTGGGC GGCCGAGGCG GGACATCGGG 
ACCATCCTGC AGATCCTCAA TGACCTCCTA AGCGCCACAC GGCACTACCA GGGCATGCCC 
CCATCACTCA CACAGCTGCG TTGCCACGCC CAGTGCACCC CAGACCTCGG CCCCAAAACT 
ACCTCCTGTG AGAAGCTGGC GGCCGCGGCC CCGGCCTCCC TGAGCCAGGC CCGCTTGTGC 
AAGCCCCCGG GGGACCGGCT GCGGCAGACG GAGAACCGAG CCACCCGCTG TAAGGTGGAG 
CGCCTTCAGC TGCTGCTCCA GCAGAAACGG CTCCGCAGAA AGGCCCGGCG TGATGCCCGC 
GGTCCCTACC ACTGGCCGCC CAGCCGCAAG GCCGGCCGCA GCGACAGCAC TGGCAGTGGG 
GGAGGTGGCA GCCCCAGCGA GGCGGCCGGC TTGGGCCTTG ACTTCGAGGA CTCCGTCTGG 
AAGCCAGAAG TGAACCCCGA CATCAAATCA GAGTTTGTGG TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005199083.1
CDS	513..2000
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_005199026.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005199026.3

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGA TGGACTGTTC CCCCGCTGCC 
AGCACTGGTG CCAGCCCCAC ATCCCCGACT GGGAGCAGCC CCACCTCCCG CTCCCGCAAA 
CCTGGCGCAG TCATCGAGAG CTTCGTGAAC CACGCCCCGG GGGTCTTCTC AGGGACCTTC 
TCTGGCACAC TGCACCCCAA CTGCCAGGAC AGCAGTGGGC GGCCGAGGCG GGACATCGGG 
ACCATCCTGC AGATCCTCAA TGACCTCCTA AGCGCCACAC GGCACTACCA GGGCATGCCC 
CCATCACTCA CACAGCTGCG TTGCCACGCC CAGTGCACCC CAGACCTCGG CCCCAAAACT 
ACCTCCTGTG AGAAGCTGGC GGCCGCGGCC CCGGCCTCCC TGAGCCAGGC CCGCTTGTGC 
AAGCCCCCGG GGGACCGGCT GCGGCAGACG GAGAACCGAG CCACCCGCTG TAAGGTGGAG 
CGCCTTCAGC TGCTGCTCCA GCAGAAACGG CTCCGCAGAA AGGCCCGGCG TGATGCCCGC 
GGTCCCTACC ACTGGCCGCC CAGCCGCAAG GCCGGCCGCA GCGACAGCAC TGGCAGTGGG 
GGAGGTGGCA GCCCCAGCGA GGCGGCCGGC TTGGGCCTTG ACTTCGAGGA CTCCGTCTGG 
AAGCCAGAAG TGAACCCCGA CATCAAATCA GAGTTTGTGG TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_005209326.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209383.1 Bos taurus midnolin (MIDN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa05459
Clone ID Related Accession (Same CDS sequence)	XM_005199026.3 , XM_005209326.3
Accession Version	XM_005209326.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGA TGGACTGTTC CCCCGCTGCC 
AGCACTGGTG CCAGCCCCAC ATCCCCGACT GGGAGCAGCC CCACCTCCCG CTCCCGCAAA 
CCTGGCGCAG TCATCGAGAG CTTCGTGAAC CACGCCCCGG GGGTCTTCTC AGGGACCTTC 
TCTGGCACAC TGCACCCCAA CTGCCAGGAC AGCAGTGGGC GGCCGAGGCG GGACATCGGG 
ACCATCCTGC AGATCCTCAA TGACCTCCTA AGCGCCACAC GGCACTACCA GGGCATGCCC 
CCATCACTCA CACAGCTGCG TTGCCACGCC CAGTGCACCC CAGACCTCGG CCCCAAAACT 
ACCTCCTGTG AGAAGCTGGC GGCCGCGGCC CCGGCCTCCC TGAGCCAGGC CCGCTTGTGC 
AAGCCCCCGG GGGACCGGCT GCGGCAGACG GAGAACCGAG CCACCCGCTG TAAGGTGGAG 
CGCCTTCAGC TGCTGCTCCA GCAGAAACGG CTCCGCAGAA AGGCCCGGCG TGATGCCCGC 
GGTCCCTACC ACTGGCCGCC CAGCCGCAAG GCCGGCCGCA GCGACAGCAC TGGCAGTGGG 
GGAGGTGGCA GCCCCAGCGA GGCGGCCGGC TTGGGCCTTG ACTTCGAGGA CTCCGTCTGG 
AAGCCAGAAG TGAACCCCGA CATCAAATCA GAGTTTGTGG TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209383.1
CDS	513..2000
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPAAPDLGRAGGGGFRKYRFILFKHPWHRQGPQNPQRGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_005209326.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209326.3

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGCCCCCA GCGGCTCCCG ACCTGGGCCG GGCTGGCGGA 
GGCGGCTTCC GGAAATACCG ATTCATTTTA TTTAAGCATC CGTGGCACCG ACAGGGACCC 
CAGAACCCAC AGAGGGGCGG CGAGAGGCCC CAGGTCAGTG ACTTCTTGTC AGGACGCTCG 
CCGCTGACCC TGGCGCTGCG TGTGGGGGAC CACATGATGT TTGTCCAGTT GCAGCTCGCA 
GCCCAGCATG CCCCGCTGCA GCACCGCCAC GTGCTGGCTG CTGCTGCTGC CGCCACCCGC 
GGGGACCCCA GCATGACCAC CACCCCTGTG TCCTCTCCCT GCCGGCCAGT GTCCAGTGCT 
GCCCGTGTCC CCCCAGTGCC CACCAGCCCT GCCCCCGCAT CTTCACCTGT CACAGCCGGC 
TCTTTCCGCT CCCATTCAGC CTCTACCACC TGCCCCGAGA TGGACTGTTC CCCCGCTGCC 
AGCACTGGTG CCAGCCCCAC ATCCCCGACT GGGAGCAGCC CCACCTCCCG CTCCCGCAAA 
CCTGGCGCAG TCATCGAGAG CTTCGTGAAC CACGCCCCGG GGGTCTTCTC AGGGACCTTC 
TCTGGCACAC TGCACCCCAA CTGCCAGGAC AGCAGTGGGC GGCCGAGGCG GGACATCGGG 
ACCATCCTGC AGATCCTCAA TGACCTCCTA AGCGCCACAC GGCACTACCA GGGCATGCCC 
CCATCACTCA CACAGCTGCG TTGCCACGCC CAGTGCACCC CAGACCTCGG CCCCAAAACT 
ACCTCCTGTG AGAAGCTGGC GGCCGCGGCC CCGGCCTCCC TGAGCCAGGC CCGCTTGTGC 
AAGCCCCCGG GGGACCGGCT GCGGCAGACG GAGAACCGAG CCACCCGCTG TAAGGTGGAG 
CGCCTTCAGC TGCTGCTCCA GCAGAAACGG CTCCGCAGAA AGGCCCGGCG TGATGCCCGC 
GGTCCCTACC ACTGGCCGCC CAGCCGCAAG GCCGGCCGCA GCGACAGCAC TGGCAGTGGG 
GGAGGTGGCA GCCCCAGCGA GGCGGCCGGC TTGGGCCTTG ACTTCGAGGA CTCCGTCTGG 
AAGCCAGAAG TGAACCCCGA CATCAAATCA GAGTTTGTGG TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_002689146.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689192.1 Bos taurus midnolin (MIDN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa05460
Clone ID Related Accession (Same CDS sequence)	XM_002689146.5 , XM_866159.7
Accession Version	XM_002689146.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689146.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGGTCAGT GACTTCTTGT CAGGACGCTC GCCGCTGACC 
CTGGCGCTGC GTGTGGGGGA CCACATGATG TTTGTCCAGT TGCAGCTCGC AGCCCAGCAT 
GCCCCGCTGC AGCACCGCCA CGTGCTGGCT GCTGCTGCTG CCGCCACCCG CGGGGACCCC 
AGCATGACCA CCACCCCTGT GTCCTCTCCC TGCCGGCCAG TGTCCAGTGC TGCCCGTGTC 
CCCCCAGTGC CCACCAGCCC TGCCCCCGCA TCTTCACCTG TCACAGCCGG CTCTTTCCGC 
TCCCATTCAG CCTCTACCAC CTGCCCCGAG CAGATGGACT GTTCCCCCGC TGCCAGCACT 
GGTGCCAGCC CCACATCCCC GACTGGGAGC AGCCCCACCT CCCGCTCCCG CAAACCTGGC 
GCAGTCATCG AGAGCTTCGT GAACCACGCC CCGGGGGTCT TCTCAGGGAC CTTCTCTGGC 
ACACTGCACC CCAACTGCCA GGACAGCAGT GGGCGGCCGA GGCGGGACAT CGGGACCATC 
CTGCAGATCC TCAATGACCT CCTAAGCGCC ACACGGCACT ACCAGGGCAT GCCCCCATCA 
CTCACACAGC TGCGTTGCCA CGCCCAGTGC ACCCCAGACC TCGGCCCCAA AACTACCTCC 
TGTGAGAAGC TGGCGGCCGC GGCCCCGGCC TCCCTGAGCC AGGCCCGCTT GTGCAAGCCC 
CCGGGGGACC GGCTGCGGCA GACGGAGAAC CGAGCCACCC GCTGTAAGGT GGAGCGCCTT 
CAGCTGCTGC TCCAGCAGAA ACGGCTCCGC AGAAAGGCCC GGCGTGATGC CCGCGGTCCC 
TACCACTGGC CGCCCAGCCG CAAGGCCGGC CGCAGCGACA GCACTGGCAG TGGGGGAGGT 
GGCAGCCCCA GCGAGGCGGC CGGCTTGGGC CTTGACTTCG AGGACTCCGT CTGGAAGCCA 
GAAGTGAACC CCGACATCAA ATCAGAGTTT GTGGTGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689192.1
CDS	503..1864
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_002689146.5
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689146.5

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGGTCAGT GACTTCTTGT CAGGACGCTC GCCGCTGACC 
CTGGCGCTGC GTGTGGGGGA CCACATGATG TTTGTCCAGT TGCAGCTCGC AGCCCAGCAT 
GCCCCGCTGC AGCACCGCCA CGTGCTGGCT GCTGCTGCTG CCGCCACCCG CGGGGACCCC 
AGCATGACCA CCACCCCTGT GTCCTCTCCC TGCCGGCCAG TGTCCAGTGC TGCCCGTGTC 
CCCCCAGTGC CCACCAGCCC TGCCCCCGCA TCTTCACCTG TCACAGCCGG CTCTTTCCGC 
TCCCATTCAG CCTCTACCAC CTGCCCCGAG CAGATGGACT GTTCCCCCGC TGCCAGCACT 
GGTGCCAGCC CCACATCCCC GACTGGGAGC AGCCCCACCT CCCGCTCCCG CAAACCTGGC 
GCAGTCATCG AGAGCTTCGT GAACCACGCC CCGGGGGTCT TCTCAGGGAC CTTCTCTGGC 
ACACTGCACC CCAACTGCCA GGACAGCAGT GGGCGGCCGA GGCGGGACAT CGGGACCATC 
CTGCAGATCC TCAATGACCT CCTAAGCGCC ACACGGCACT ACCAGGGCAT GCCCCCATCA 
CTCACACAGC TGCGTTGCCA CGCCCAGTGC ACCCCAGACC TCGGCCCCAA AACTACCTCC 
TGTGAGAAGC TGGCGGCCGC GGCCCCGGCC TCCCTGAGCC AGGCCCGCTT GTGCAAGCCC 
CCGGGGGACC GGCTGCGGCA GACGGAGAAC CGAGCCACCC GCTGTAAGGT GGAGCGCCTT 
CAGCTGCTGC TCCAGCAGAA ACGGCTCCGC AGAAAGGCCC GGCGTGATGC CCGCGGTCCC 
TACCACTGGC CGCCCAGCCG CAAGGCCGGC CGCAGCGACA GCACTGGCAG TGGGGGAGGT 
GGCAGCCCCA GCGAGGCGGC CGGCTTGGGC CTTGACTTCG AGGACTCCGT CTGGAAGCCA 
GAAGTGAACC CCGACATCAA ATCAGAGTTT GTGGTGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIDN ( XM_866159.7 ) cDNA ORF clone, Bos taurus(cattle) -> XP_871252.2 Bos taurus midnolin (MIDN), transcript variant X4, mRNA.

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CloneID	OBa05460
Clone ID Related Accession (Same CDS sequence)	XM_002689146.5 , XM_866159.7
Accession Version	XM_866159.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midnolin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_866159.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGGTCAGT GACTTCTTGT CAGGACGCTC GCCGCTGACC 
CTGGCGCTGC GTGTGGGGGA CCACATGATG TTTGTCCAGT TGCAGCTCGC AGCCCAGCAT 
GCCCCGCTGC AGCACCGCCA CGTGCTGGCT GCTGCTGCTG CCGCCACCCG CGGGGACCCC 
AGCATGACCA CCACCCCTGT GTCCTCTCCC TGCCGGCCAG TGTCCAGTGC TGCCCGTGTC 
CCCCCAGTGC CCACCAGCCC TGCCCCCGCA TCTTCACCTG TCACAGCCGG CTCTTTCCGC 
TCCCATTCAG CCTCTACCAC CTGCCCCGAG CAGATGGACT GTTCCCCCGC TGCCAGCACT 
GGTGCCAGCC CCACATCCCC GACTGGGAGC AGCCCCACCT CCCGCTCCCG CAAACCTGGC 
GCAGTCATCG AGAGCTTCGT GAACCACGCC CCGGGGGTCT TCTCAGGGAC CTTCTCTGGC 
ACACTGCACC CCAACTGCCA GGACAGCAGT GGGCGGCCGA GGCGGGACAT CGGGACCATC 
CTGCAGATCC TCAATGACCT CCTAAGCGCC ACACGGCACT ACCAGGGCAT GCCCCCATCA 
CTCACACAGC TGCGTTGCCA CGCCCAGTGC ACCCCAGACC TCGGCCCCAA AACTACCTCC 
TGTGAGAAGC TGGCGGCCGC GGCCCCGGCC TCCCTGAGCC AGGCCCGCTT GTGCAAGCCC 
CCGGGGGACC GGCTGCGGCA GACGGAGAAC CGAGCCACCC GCTGTAAGGT GGAGCGCCTT 
CAGCTGCTGC TCCAGCAGAA ACGGCTCCGC AGAAAGGCCC GGCGTGATGC CCGCGGTCCC 
TACCACTGGC CGCCCAGCCG CAAGGCCGGC CGCAGCGACA GCACTGGCAG TGGGGGAGGT 
GGCAGCCCCA GCGAGGCGGC CGGCTTGGGC CTTGACTTCG AGGACTCCGT CTGGAAGCCA 
GAAGTGAACC CCGACATCAA ATCAGAGTTT GTGGTGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_871252.2
CDS	503..1864
Translation	MEPQPGGARSCRRGAPGGACELSPTAETAPMSLAIHSTTGTRYELSVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAATRGDPSMTTTPVSSPCRPVSSAARVPPVPTSPAPASSPVTAGSFRSHSASTTCPEQMDCSPAASTGASPTSPTGSSPTSRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCTPDLGPKTTSCEKLAAAAPASLSQARLCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWPPSRKAGRSDSTGSGGGGSPSEAAGLGLDFEDSVWKPEVNPDIKSEFVVA

Target ORF information:

RefSeq Version	XM_866159.7
Organism	Bos taurus(cattle)
Definition	Bos taurus midnolin (MIDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_866159.7

ORF Insert Sequence:

ATGGAGCCGC AGCCCGGCGG CGCCCGGAGC TGCCGGCGCG GGGCCCCCGG CGGCGCCTGC 
GAGCTGAGCC CGACGGCCGA GACGGCGCCC ATGAGCCTGG CCATCCACAG CACGACAGGC 
ACCCGCTACG AGCTGTCGGT GCCCCCCGAC GAGACGGTGG AGGGGCTGCG CAAGCGGCTG 
TCCCAGCGCC TCAAAGTGCC CAAGGAGCGC CTGGCGCTGC TCCACAAAGA CACCCGGCTC 
AGTTCGGGGA AGCTGCAGGA GTTCGGCGTG GGGGATGGCA GCAAGCTGAC GCTCGTGCCC 
ACCGTGGAGG CGGGCCTCAT GTCTCAGGCC TCCAGGCCAG AACAATCTGT GATGCAGGCC 
CTGGAGAGCT TGACAGAGAC CCAGGTCAGT GACTTCTTGT CAGGACGCTC GCCGCTGACC 
CTGGCGCTGC GTGTGGGGGA CCACATGATG TTTGTCCAGT TGCAGCTCGC AGCCCAGCAT 
GCCCCGCTGC AGCACCGCCA CGTGCTGGCT GCTGCTGCTG CCGCCACCCG CGGGGACCCC 
AGCATGACCA CCACCCCTGT GTCCTCTCCC TGCCGGCCAG TGTCCAGTGC TGCCCGTGTC 
CCCCCAGTGC CCACCAGCCC TGCCCCCGCA TCTTCACCTG TCACAGCCGG CTCTTTCCGC 
TCCCATTCAG CCTCTACCAC CTGCCCCGAG CAGATGGACT GTTCCCCCGC TGCCAGCACT 
GGTGCCAGCC CCACATCCCC GACTGGGAGC AGCCCCACCT CCCGCTCCCG CAAACCTGGC 
GCAGTCATCG AGAGCTTCGT GAACCACGCC CCGGGGGTCT TCTCAGGGAC CTTCTCTGGC 
ACACTGCACC CCAACTGCCA GGACAGCAGT GGGCGGCCGA GGCGGGACAT CGGGACCATC 
CTGCAGATCC TCAATGACCT CCTAAGCGCC ACACGGCACT ACCAGGGCAT GCCCCCATCA 
CTCACACAGC TGCGTTGCCA CGCCCAGTGC ACCCCAGACC TCGGCCCCAA AACTACCTCC 
TGTGAGAAGC TGGCGGCCGC GGCCCCGGCC TCCCTGAGCC AGGCCCGCTT GTGCAAGCCC 
CCGGGGGACC GGCTGCGGCA GACGGAGAAC CGAGCCACCC GCTGTAAGGT GGAGCGCCTT 
CAGCTGCTGC TCCAGCAGAA ACGGCTCCGC AGAAAGGCCC GGCGTGATGC CCGCGGTCCC 
TACCACTGGC CGCCCAGCCG CAAGGCCGGC CGCAGCGACA GCACTGGCAG TGGGGGAGGT 
GGCAGCCCCA GCGAGGCGGC CGGCTTGGGC CTTGACTTCG AGGACTCCGT CTGGAAGCCA 
GAAGTGAACC CCGACATCAA ATCAGAGTTT GTGGTGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MIDN
Entrez Gene ID	523615
Full Name	midnolin
General protein information	Preferred Names midnolin Names midnolin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Xenopus tropicalis (tropical clawed frog)	midn	NP_001015832.1
Bos taurus (cattle)	MIDN	XP_005209382.1
Mus musculus (house mouse)	Midn	NP_067540.1
Danio rerio (zebrafish)	midn	NP_996935.1
Homo sapiens (human)	MIDN	NP_796375.3
	LOC721215	XP_001117294.2
Canis lupus familiaris (dog)	MIDN	XP_005633205.1
Pan troglodytes (chimpanzee)	MIDN	XP_001172401.3
Rattus norvegicus (Norway rat)	Midn	NP_001178506.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MIDN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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